LOCUS       DAOTAC010000001        25374 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_1602_length_25374_cov_4.939018, whole genome shotgun sequence.
ACCESSION   DAOTAC010000001 DAOTAC010000000
VERSION     DAOTAC010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 25374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25374
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1602_length_25374_cov_4.939018"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1980
                     /locus_tag="PLM74_00005"
     CDS             <1..1980
                     /locus_tag="PLM74_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HON41497.1"
                     /translation="NIIVMVDEAHRTQYDILAMNMRNALPNAAFIAFTGTPLMAGEER
                     TREVFGDYVSVYNFAQSVEDSATVPLYYENRIPRLQLTNEALNDDLNELLDSAALDEE
                     QEKKLEREFARQYHLITRDDRLEAIAEDIVAHFMGRGDMGKAMVVSVDKATAVRMYDK
                     VGKYWRQYLDELRAEYRRSSGQKRDTLAERVKFMEETDMAVVVSSAQNEVEDFAAKGL
                     DILTHRKRMINEQLDTKFKNPDDPLRIVFVCAMWITGFDVPCLSTIYLDKPMKNHTLM
                     QTIARANRVFKDKTNGLIVDYIGIFRDLQRALAIYGTAQGGAAGEGDMPIKDKDELVG
                     ALGTALEEAEKHCRRFNVDPEVILASQGNECAALIADAADSLLETEESRKEYMSLAGD
                     AIKLYKAILPDPRASEFQARVTLLAVLVARIRSLAPQTDISQVMEGVNALLDDSIATD
                     GYRISDPVSVVDLSQIDFEALRKKFDEGRKRTIAEQLKGAINSKLQKMVRLNKSRTDF
                     ADKFQKLIDDYNAACLAGNPDIDDFFGKLVEFTKELDAEERRGIAEQLSEEELAMFDI
                     LTKPHINLDEKEKSQVKQVARDLLETLKTSKLVLDWRKRQQSRAQVRQAIDIKLDELP
                     RCYTKEIYRQKCDDVFRHVYDSYYGAGQSIYSQAG"
     gene            2089..2859
                     /locus_tag="PLM74_00010"
     CDS             2089..2859
                     /locus_tag="PLM74_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HON41498.1"
                     /translation="MTVKREFPILEFDPASPAKIQPSNIIRKRNVPKHCVITFFGDVI
                     GRMLSEGRLSKIAEFHTATVILPIYRTEYSGKPIGIVQGYLGAAGSGGQLEELIAMGF
                     ERFIVCGAAGVLQKGVQVGHLVVPYSAIRDEGLSYHYLEPSREVECSKQALKCITDYL
                     EINRIPFIKAKTWTTDAFYRETEDKIALRISEGCVTVEMEAAAFFAVSKFRNVVLGQI
                     LHGGDDLSGIEWDARNWASREDIRANLVDLSIAICLEL"
     gene            3071..4801
                     /locus_tag="PLM74_00015"
     CDS             3071..4801
                     /locus_tag="PLM74_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013921436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON41499.1"
                     /translation="MKRLAVYMKPYIVFFLVAPVFKAIEASAELLQPKIMTTTVNAGI
                     IAGDVSVIWRNGLLMLGVALVGIAGGVGACWFAAKSSQGFGADLRRAVFQKVQSFSFS
                     DLDRFSTGSLVTRLTNDITQLENTSLMMQRIMVRAPLLCIGAIIMTISIQPRLAVALV
                     SMAVALLCAVLFIMGRAFPLFGQVQQCLDAVNTTMQENLAGMRTVKSFVRAGFEVERF
                     DRVNERLYNTSVAAGRVMAFSQPTVYFGMNIAALIVLWRGGRLVAGREMEVGQIMAVM
                     QYITQILFSLSMLGFLLINLVRARASALRVIEVLEVEPKIRSSPEARRDACVTRGEVE
                     FRNVTFRYPGAVGDPVLSGVSFHLRPGETLGVLGGTGSGKSTLVHLIPRLYDVTNGQL
                     LVDGVDVRDYVLESLRAGIGMVLQDTVLFSGTIEDNLRWGAPDASCESLQKAASQAQA
                     TQFIDKMPLGYGSPIGQRGAGLSGGQRQRLSIARALVKNPSILILDDATSAVDTRTEV
                     ALRQALREERRAATTIIIAQRVTSVMGADRILVLENGRVSAIGRHEELLESCPAYREL
                     CRLQLGTEAV"
     gene            4803..6701
                     /locus_tag="PLM74_00020"
     CDS             4803..6701
                     /locus_tag="PLM74_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009675830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON41500.1"
                     /translation="MSGSTPREGSDRRPGEFGPQVGSQLGPRFGSRASRLGPVSKPKD
                     IGAVLAHLWVYLRQQRRTLALVFAATLVSSLVSIFGPRLIGTAIDPYVLDGDLLGLSR
                     ILILLSAMYASSSLASWVQQTCMVGLSNRALREMRHDLFAKVQALPLSFFDSATHGDL
                     MSRLANDVDTVGMALGQPIVQLFSSVVSISGTLIIMFSMSVWMALTVLAMVPVTLLVT
                     TKVAGWSRKLYRRRQEALGEINGYIEEMVTGARVVKVFGREDDACAAFNDKSERLRDI
                     AYRTELVSGMFMPAMHLLDNLSFAVVALIGALLAANGIIHLGIVASFIIYSRQFMQPV
                     RDLSNQWNTFQSGLAGAERVFEIMDTESEADAPGANDLRLPVSGHVEFERVSFGYKPG
                     VPVLTDISLEAKPGQTIALVGATGSGKTTIISLLSRFYDIEEGHIYVDGVDVREIRRE
                     SLRRALGIVLQDSQLFSGTVRENIRYGRPDASDADVEKAAELALADPFIRRMPEGYDS
                     VLTADGGNLSHGQRQLLCIARAVLADPAILILDEATSSVDTRTELRIQQAILRLMRGR
                     TSFVIAHRLSTIQGADQILVIDHGRIVERGAHDELLARGGAYARLHESQFHVAAQPAM
                     GHGIFESV"
     gene            complement(7166..8752)
                     /locus_tag="PLM74_00025"
     CDS             complement(7166..8752)
                     /locus_tag="PLM74_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013920931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON41501.1"
                     /translation="MGSTSGPRIKHVKMRQITKTFPGVRANDCVDLDINSGEVLALLG
                     ENGAGKTTLMKVLYGLYRPDSGQILVNGEAVTINSPRDAIALGIGMVHQHFMLVPSLT
                     VVENVVLGLQSARGPLLDLDTASQRIVELGDEYGFRIRPDAPVWQLSVGEQQRVEIIK
                     ALYRGAELLILDEPTAVLTPQEAGELIAILRGMAQAGKSLVFISHKMNEVMAVSDRVT
                     VLRAGAVVGTVPTSQASPESLATMMVGRKIEAAKRTGEASHGQPVLELDQVWADADNG
                     LPALKGLSLAVREGEILGLAGVSGNGQRELAEIMTGLRRVSRGKVRLRGHDLTGASPA
                     AMIDAGLGYVPEDRLSFGIIQSFSVEENLILKDHHRPPFARWIFLQNDVIRNWSRKTV
                     ETFDVRTPSVETRAGSLSGGNIQKVILARDITRKPAVLLACYPTRGLDIAATEYVHRR
                     LLDVRDSGGGILLISEELDEILNLSDRIAVIYEGKIVAVVEADSVSLEELGLLMAGAN
                     RPAGADADANADNAALSLQG"
     gene            complement(8827..9894)
                     /locus_tag="PLM74_00030"
     CDS             complement(8827..9894)
                     /locus_tag="PLM74_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009620533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HON41502.1"
                     /translation="MSSSTAAAVDAQIRQEVSTVRKNRIVLVAFLALATLMVFGAGYG
                     YGAPAKKIRIAMLMASTIDDMAWSQSMYEGLKAVQKEMGADKLEFVTSERMGNPVDAG
                     AAIRQYADQGFDIIIAHGAQYQSVLMELAPDFPKTTFAYGTGFTTAPNIFAYDPQAQE
                     GAYLLGMVAALMSKTGVVGIVGPVESGDAIKYNYGFQQGVAAGNPNVKVRIAYTGSFG
                     DIVGAGELAKVHMDAKADVLTGSSQQAVGAARAVAERSGVYWMSTDMDQASLAPDHVL
                     AAQVYHWEKVVKDMINYRAQGILGGKHLTLTFADGTLELRFNPKLTNKIPKAVLDRVE
                     KAKQDIISGKLKVELPKAGGK"
     gene            complement(9922..10854)
                     /locus_tag="PLM74_00035"
     CDS             complement(9922..10854)
                     /locus_tag="PLM74_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HON41503.1"
                     /translation="MATISSQGIIVGILATGIRLAAPFLYASLGEMFAQRSGVFNLGL
                     EGIMMMGAFAGFMTSLGTGSPWLGVLAAIGVGAALGLLMAFISVTMQAEQGISGIGLH
                     MFCWGLSGLLFRVYLGFITSCKGFGPWNIPVLSKIPFLGPVLFQHNWMVYFSLLLVPV
                     SWFVLYRTTWGLKVRAVGTTPQAADTLGVNVNAIRYQCVVLGGAMAGLAGAFLTVGQA
                     NMYADNITAGRGFIAVALVYFGRWSPVGILLGSLLFSMMNALQLWIQVLGISFPYEFA
                     VILPYVMTIAALAVAVRRVWAPTALGKPFRREAG"
     gene            complement(10856..11992)
                     /locus_tag="PLM74_00040"
     CDS             complement(10856..11992)
                     /locus_tag="PLM74_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014904365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HON41504.1"
                     /translation="MKKRTGTSDSLLQAGVLVGALVLALLVGAGLILLARANPITAYK
                     AMFTGPVSGKYGITESLVKATPLLLVGLGIVISFRSGILNIGGEGQMVAGSLAGAAVA
                     LAFPSWPAIVLLPMTLIAGVLAGALWGGIAGWLKARLSVNEILSTVMLNQIAFQLNLY
                     LIRGPMIDPQEVAYGTGCPQTALLPRSIWLPRIIRGTRFHWGLVIAIAFAILVYIFLW
                     RTTIGYRMRASGAGPQAAKAAGIRVENYQILAMAFAGGFAGLAGIVEVLGVHHRVLEG
                     LSGGYGFSGIVVALFGRLHPLGCIPASILFGALTLGADMMQRAVQIPAAIVMAIQGLV
                     VLFVVGSDLLIRRPDLLRSRRMSGGSAGVAGKQGMARQGKGVSN"
     gene            12306..13856
                     /locus_tag="PLM74_00045"
     CDS             12306..13856
                     /locus_tag="PLM74_00045"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HON41505.1"
                     /translation="MSSLERSISILKDAFPEAFTEGRIDFDILRGLLGVCVDDRHERY
                     RLTWNGKGRARRIAQAPSVGALQPSPEESVDWDTTQNLLIEGDNLEVLKLLLNEYQGK
                     IKMIYIDPPYNTGNDFIYRDDFRDSVASYLQLTGQADASGRLLRADAETSGRYHTDWL
                     NMMYPRLKLARNLLCEDGVIFISIGDNEVANLRIICDELFGEGSCLGCVARVAKKTSN
                     KGTHFAPSKDYVLVYACRAECVAPFMDVVDPAYASRFRERDGRGRYATVGLYQAALDP
                     RPNQRYWIACPDGSFAIPPGEVFPDEVADGAQVKPASRRDRVWRWSRESYLAKKHLLV
                     FKKSARSPLVTPDGSPSKWNVYTKYYLEDRLNDGIRPRDYLDDITNDLGTAALKSLGL
                     DDFFEFAKPPELVRRLMQWLGGRDGIYLDFFAGSGTTAQAVLEQNAEDGGSRRFILVQ
                     LPEPCPHDSVAAQNGYNTIFDICKERIRRVIARLRADEAHGTALDLGYRVFKLRGEAV
                     EDQVRPRS"
     gene            13828..16116
                     /locus_tag="PLM74_00050"
     CDS             13828..16116
                     /locus_tag="PLM74_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005224766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HON41506.1"
                     /translation="MKIKFDPDLDYQRDAIDSVVQIFAGQEDCRADFAGDLVIGNRLR
                     LSEEEILKNVRAIQLCNGLALSDTVSGMNFTVEMETGTGKTYVYLRTIFELNREYGFL
                     KFIIVVPSIAIKEGVAKSLQMVEEHMRGLYDNVRFDHFIYDPASLSQVRNFATSDYIQ
                     IMIINIDAFRKSFVDPEKEHRANIIHRSHDRMMGARPIEFIQGTSPIVIIDEPQSVDT
                     TAKSAEAIASLHPLCTLRYSATHVERHNMMYRLDPVDAYQRKLVKQVEVASVEASHGP
                     SRPYVRLLGVDNTRGSITARIELDALHRGGAVRRVARSVRCGDDLQEVSGSRDVYTGY
                     IVENICCERGNEHIMLARWPEAVRLNQPIGDLDDGEYKRIQIRTTIREHLDKELMLRP
                     RGVKVLSLFFIDRVANYRWYDEDGNPRKGKYAQMFEEEYVKAMGDVKYRSLPEGADPA
                     AAAESVHNGYFAIDRRRDAAGRDMFRESRGDGVAQADESAYQLIMRDKEKLASFESGL
                     KFIFSHSALREGWDNPNVFQICTLNDTVSAMKKRQEIGRGLRLAVNQDGERVYGFDVN
                     TLTVVANQSYAEFVRQLQSEIEEDTGVRLGEARRLNVKNAAERRQVGPGGSNGVGTSP
                     EPGPKQECNTGYRVTFDTDELIRRCAGEIRRMPAIDHTGPMIRKARLGMGRGGIGAGD
                     VRVSAEIHEAHGHPLPDIVTQLQNDTSLTRRTIVKILVQSGRLEDFKAGPQVFIEKVS
                     AIIRRRVGLCIQEGGTHAGTGS"
     gene            16097..17968
                     /locus_tag="PLM74_00055"
     CDS             16097..17968
                     /locus_tag="PLM74_00055"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HON41507.1"
                     /translation="MQGLDPSIGQSLGSERYSFTWNGKRRAGLIAQAPPDGTLRPCPE
                     QSVNWDTTRNLFIEGDNLEVLKLLQKSYHKKVKMIYIDPPYNTGKDFVYRDDFTDSIQ
                     NYLRLTGQVDEAGRRLSANPETCGRYHTNWLNMMYPRMRLARNLLRDDGVIAISIDDH
                     ELANLRKMCDEVFGEENFCGVFIWEKKKKPLFLDANMGTVTEYIICFAKNRAQSPPFG
                     AGAAREGKKYPFNNAGNGVQTLTFPAGSVRFGFGDQVVAAQDMSEGSIFTELLDDVAI
                     VGGRNANAFRLRGEWRYSQQKLDEFVQEGADIVISKIPFRPNYVSRSGELKKTSNLLS
                     YRTNGVPTYEDATQEIRALFGADVMSYPKPSGLMKYLVRAMTGEGDIVMDFFAGSGST
                     AHGVMLQNSEDGYNRQYILVQLPEPLKPDDAESGPAFEFCRAHGLSPTIAEIGKERIR
                     RAATAAAAGAVERGALGLGPEFGPGLGAGRGSGLDLGFRVFRLDSSNLWPWDSDPRNL
                     EESLLQAVERIKDDRQEADVLYELVIQYGLDLAASVERREIDGKAVYIVGEGELVVCL
                     ADHISSRLALGIARLRAELAAAQMQVVLRDAGFDSDADRINTIQVLRGAGVESAKTL"
     gene            18627..21287
                     /locus_tag="PLM74_00060"
     CDS             18627..21287
                     /locus_tag="PLM74_00060"
                     /inference="COORDINATES: protein motif:HMM:NF028468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE3 domain-containing protein"
                     /protein_id="HON41508.1"
                     /translation="MRDVETQTVSAALLPVGKSGVSRVRSPDKVSWPSHVYVGILVWA
                     LIVPVGWVVLGSSTPALAFSFETASIFFAVLAFFTAWHTYGYSRSSYLLFIGMTQLIV
                     ALLDSFSLISSPVLRLSIELGIQHQMQFWAAARTIHAFSMFLAPRYARLRRPSIRMLA
                     GSALLAIALVAAIVWLPGFPELATVDGALTPDMVVINYLILGVLALSVIGQTLSDVRD
                     RVLRELMTASLAVAVVSQASFTVAGPITNYMTAGAGLFGHIAKALSYWLVYEAVVIGG
                     YEHPLARLRDGLRHVRATRRVTLSSVGDGAITTNKDGKVTLLNPEASRITGWTEHDAL
                     GVDIDSVFRLSLMGAGSSAGRRSRSTCLTESVVYAKDASIRYVEYTSSPIHGPDGGDL
                     GEVIVFREISERVQKDELIKTSMEKYRALFEHMPNGVAVYRSVDGGKDFVFVEFNRAG
                     ELIEGIDAASVVGRRLTEVFPGLAKMGLMEALQRVWLTGHSERLSPAYCENGPRSGWR
                     ENWIYRVPSGEVVAVFQDVTERVQAEQRVEYLTVHDALTGLYNRRHLEQVVSQLDVEA
                     VVPVSVVFGDANGLKLANDAFGHEAGDRLLVNIAKVLQACSRSDDIIVRMGGDEFVVI
                     MPGVREGEAAEAVVRMRRAFDEAPADPVRVSLALGSSTRSTPSVSLREVLADAEKHAY
                     RQKLAESKSARGSLMKSLLRTLAVKTPETEDHVLRMRELALELGRNLGLPDSTLDDLA
                     VLTLIHDIGMVGIPDSILTKVDDPLSHDEQEALKKHPEIGYRVAQASIELAHVADGIL
                     SHHENWDGTGYPQGLKGEEIPLIARIVAIVDAYDSMTAGRSYKAARSKKEAIDEIRKL
                     AGARFDPDLAEAFARVISGHEGFRFGTIER"
     gene            21357..22556
                     /locus_tag="PLM74_00065"
     CDS             21357..22556
                     /locus_tag="PLM74_00065"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HON41509.1"
                     /translation="MVPDRSLAWLAILTFVVSFSSGMTGPVFPLHAQALGATYEQIGM
                     VSAAGSLIHAGFASRAGRLADQLGHDLMFLLSSLTIVLSTTAYFFSRTLLMAGLGKAL
                     DSLFIASYWPALESASHSSGSAAGHSLGVVYTVYPMATFAASAATGWLAGRLGYRASF
                     AAALVGGVAAVVGVSIGLSRRRRLDAGYGDATVRVGRRLRARGERSKGNVRLLPGSGL
                     RFAAALTACFTYCVLVGEAFTFVPLLAEARGLRVELVGLLVGVFWLGRTATSLPAGML
                     ADRVGVDVVLIPAFIVGGIGSAVVAWHAAPVVMFVGVTMMGLCAGAVAPVAMVLGAQC
                     VDPGSHGWALGLCETFCGIGFISTGLVGGVLAGRCGLAVPFAAGAAVCTAVALGLALA
                     SRRGRRS"
     gene            22688..23041
                     /locus_tag="PLM74_00070"
     CDS             22688..23041
                     /locus_tag="PLM74_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41510.1"
                     /translation="MNGWMCPTSPYGGYGAYPYRRWRDPEGEYEWEERHMLREIYRGM
                     HHLLREEAEEAHKIDEIYHLCHDIARMVAGQRPMEMPETVMPPMPMVPPMAPSPVVAP
                     GPMYPCPSRPPMGVI"
     gene            23379..24800
                     /locus_tag="PLM74_00075"
     CDS             23379..24800
                     /locus_tag="PLM74_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HON41511.1"
                     /translation="MISSVDIVKPLVNQALAYVSKDPVANLPKILKIGEKIAGKESHK
                     AQVRDVTRVLTESDGNWRELAIRLLTETHPNIMKSIGVNFFVNASLVGIPKQYKISEE
                     LGVQVPYAILMDPTEKCNLRCTGCWAGDYQRVRELDYEVMDRVCREAEELGIYLIVVS
                     GGEPMVAKDKLLRLAESHPNQLFHPFTNGTLIDEDFVSEMVRLGNIAPALSVEGLEQE
                     TDNRRGKGVYKKIMHAMDLMREAGCIFGFSACYARNNVDVIASDEFIDTMVDKGCTFG
                     WFFTYVPVGSEPNLDLMATPEQRAKMYDAVRSFRNTKPIFLVDFWNDGEFSLGCIAGG
                     RRYLHINAAGDVEPCAFIHYATDNINDVSLKQALASPLMRAYQKRQPFNENMLRPCPL
                     IDNPEKLVEIIQESGARSTQLGEHAVEPEVLADKIQKEYTQHWAARADELWQSNPHPF
                     HDRACEFAKKEEAERRERQASRV"
     gene            complement(24885..>25374)
                     /locus_tag="PLM74_00080"
     CDS             complement(24885..>25374)
                     /locus_tag="PLM74_00080"
                     /inference="COORDINATES: protein motif:HMM:NF015042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41512.1"
                     /translation="FAKAAAAVGVGIHERPALGAPGPIVSPDGTIIGEHMGTAMYTVG
                     QRRGLGQLQSKHDGPMYVIEIRPESNTIVAGPREYLYSTEFRAEQVNFIPFDVLEDEM
                     EAQVQIRYKSAPAKALLTPAADGNVRVKFASPQRAITPGQSGVFYSGDMLLGGGIIAS
                     ST"
BASE COUNT         5295 a         7038 c         7845 g         5196 t
ORIGIN      
        1 aacatcattg tcatggtgga cgaagcccac aggacgcagt atgacatcct ggcaatgaac
       61 atgagaaatg ccctgcccaa cgccgccttc atcgctttca ccggcacacc gctcatggct
      121 ggcgaagaac gcacccgcga ggtcttcggc gattacgtga gcgtatacaa cttcgctcag
      181 tccgttgagg atagcgccac tgtccccctg tactatgaga accgcatacc caggctccag
      241 ctgaccaacg aggcgctcaa cgacgaccta aatgaactgc ttgacagtgc tgctctcgat
      301 gaggaacaag agaagaagct ggagcgggag ttcgcccgcc agtaccacct gatcaccagg
      361 gatgacaggc tcgaagctat cgcagaggac atagtggccc acttcatggg cagaggcgat
      421 atgggcaagg ccatggtggt gtcagtagac aaggctaccg ctgtgcggat gtacgataaa
      481 gtgggcaagt actggcggca gtatctggat gaactcaggg ccgaataccg gcgctcatca
      541 ggccaaaagc gcgatacgct ggcggagcgg gtcaagttca tggaggagac agacatggcc
      601 gtcgtggtgt cgagcgctca gaacgaagta gaggacttcg ccgccaaggg cctggacatc
      661 ttgactcacc gtaagcgcat gatcaatgag caactggaca ccaagttcaa gaaccctgac
      721 gatccgctgc gcatcgtatt cgtgtgcgct atgtggatca ccggctttga cgtgccatgc
      781 ctgtctacca tctacctaga caagccgatg aagaaccaca cgttaatgca gacgattgcc
      841 agggccaaca gagtgttcaa agacaagacc aatggcctca ttgtggacta cataggcatc
      901 ttccgagatc tccagcgggc gctcgctatc tacggcaccg cgcagggcgg cgctgccggc
      961 gaaggggaca tgccaatcaa ggacaaggat gaactcgtag gggcgcttgg gacggctctg
     1021 gaagaggccg agaaacactg ccgaagattc aacgtggacc cggaggtcat cctcgcctcg
     1081 cagggcaacg aatgtgcggc gttgatcgca gatgcggcag actctctgct cgagaccgag
     1141 gaatccagga aggaatacat gtcgcttgcc ggcgatgcca tcaagctgta caaggccata
     1201 ctgcctgacc caagggcatc cgaattccag gccagagtca cgcttcttgc tgtactcgta
     1261 gccagaattc gctcgttagc ccctcaaacc gacatttcgc aggtcatgga aggggtcaat
     1321 gcgttgcttg acgactccat agccaccgac ggctaccgga ttagcgatcc tgtgtcagta
     1381 gtcgacctga gccagatcga tttcgaggct ctcaggaaga agttcgatga gggacggaaa
     1441 cggaccatcg ctgaacaact caaaggggcc atcaacagca aattgcagaa gatggttcgg
     1501 ctcaataagt cccgtacgga ttttgcggac aaattccaga agctcataga cgactacaat
     1561 gccgcatgcc tcgcaggaaa tcccgacatc gacgacttct tcggcaagct tgtggagttc
     1621 accaaagagc tggacgcgga ggagagacgc ggcatcgccg agcagctatc agaagaggag
     1681 ctcgcgatgt tcgacatctt gaccaagcct catataaacc ttgacgagaa ggagaagagc
     1741 caggtcaagc aggtagctcg ggatcttctc gaaaccctta agacaagcaa gctcgtgctg
     1801 gattggcgca agcgtcagca atccagggcc caggttaggc aggccataga catcaagctc
     1861 gacgagcttc cgagatgcta caccaaagag atctacaggc agaagtgtga tgacgtcttc
     1921 cgccacgtgt acgactcgta ctacggagct gggcagagca tatatagcca ggctgggtag
     1981 cgtgggttgc gctggagacc ggcgcgagcc tgcatccggg tccttcttgc cacagtgcgg
     2041 gtacaatgta gcagtacaca agatctggct gccgaggagg agatcacaat gaccgtaaag
     2101 agggaattcc ccatattgga gtttgacccc gcctcgccgg cgaagatcca gccttccaac
     2161 atcatcagaa aacgtaatgt gccaaagcat tgcgtgatta cgtttttcgg tgacgttatt
     2221 gggagaatgc ttagtgaggg aagactaagc aaaatcgcgg agtttcatac agcaacagtc
     2281 atattgccta tctatcgcac cgagtatagc ggaaaaccta tcgggattgt gcagggttat
     2341 ctgggagcag caggttccgg cgggcaactt gaagagctaa tcgccatggg atttgagaga
     2401 tttatcgtat gtggtgctgc cggggtattg caaaagggcg ttcaagtagg tcatctggtt
     2461 gttccatatt cagcaatcag ggacgaaggc ctttcatatc actacctaga gccgtctagg
     2521 gaagtcgaat gtagcaagca agcgctgaaa tgcattaccg attatctgga gatcaaccgc
     2581 attccattca tcaaagccaa aacgtggact accgatgcgt tttaccgtga aacagaagac
     2641 aaaatagcct tgcgcatttc tgagggatgc gtgactgtag aaatggaagc cgcagcattt
     2701 ttcgcagtat cgaaattcag aaatgttgtg ctcggacaga ttcttcatgg cggagatgat
     2761 ctgagtggca ttgaatggga tgcaaggaat tgggcgagta gggaagacat cagggccaat
     2821 ctagtcgatt tgtccatagc catttgcctg gagctataga tcacctgata gccgtgaagg
     2881 gagaaaagcc atcgatcaag attggcggcc gggcacactc actcatgctc cacgggctgc
     2941 atagggacgg tcgcggactc gcgtacacac gattaacttc ccccctcata cgtgctacat
     3001 tatcaatgta tctatgattc tggcctgctc aaacagcttg tgctgccagt agccccgggg
     3061 ggccgtctat gtgaaaagac ttgcggtata catgaagcct tacattgtct tctttcttgt
     3121 tgcgcctgtc tttaaggcca ttgaggcatc ggctgaactg cttcagccca agatcatgac
     3181 cactacggtg aatgcgggca tcatagcagg cgatgttagt gtcatctggc gaaatgggct
     3241 gcttatgctg ggtgtcgcgc tggtggggat tgcgggcggc gtcggtgcat gttggtttgc
     3301 cgccaagtcg tcgcaggggt tcggcgctga tctgcggcgt gcggtgtttc aaaaggttca
     3361 gagcttctcc ttttctgacc tcgaccggtt ctccacgggc tcactcgtca cacgtctcac
     3421 caatgacatc acccagttgg agaatacgtc cctcatgatg cagcggatca tggttagggc
     3481 gcccctactc tgcataggcg cgatcatcat gaccatctcg atccagcctc gtcttgctgt
     3541 ggcgcttgtg tcaatggcag tggcgctgct atgtgctgtt ctttttatta tgggccgggc
     3601 gtttcccctg ttcggccagg tgcagcagtg cctggacgct gtcaatacta cgatgcagga
     3661 gaaccttgcc gggatgcgta cggtcaaatc gttcgtccga gccggcttcg aagtcgagcg
     3721 cttcgaccgc gtcaatgagc ggctctacaa cacgagtgtc gctgcgggca gggtgatggc
     3781 atttagccag cctacggtgt atttcggtat gaacattgcg gccctgatag tcctgtggcg
     3841 gggcggccgc ctggttgccg gccgcgagat ggaagtcggc cagatcatgg cggtcatgca
     3901 gtacatcact cagattctct tctccctgtc gatgcttgga ttcctcctga tcaacctggt
     3961 ccgggcccgc gcgtcggccc ttcgcgtcat cgaagtcctc gaagtagagc ccaagatacg
     4021 gagcagcccg gaggcccgcc gcgatgcgtg tgtcacccgg ggtgaggtgg agtttcgcaa
     4081 tgtcacgttt aggtacccag gcgccgtcgg agatccggtc ctcagcggtg tctcgtttca
     4141 tctacgcccc ggcgagaccc ttggcgtgct cggcggaacc ggctcgggca agtcgacact
     4201 tgtccactta ataccgcgtc tgtacgatgt aacgaatggc cagctcctgg tggacggggt
     4261 agatgtgcgc gattacgtcc tggagagtct gcgggccggc ataggcatgg tgctgcagga
     4321 cacggtgctc ttctccggga cgatagagga caacttgcgg tggggagcgc ctgacgcgtc
     4381 ctgcgaaagc cttcaaaagg ccgcctcaca ggcacaggcc acgcagttca tcgacaagat
     4441 gccgctggga tatggaagcc cgatagggca gcggggagcc gggctctcag gcggccagcg
     4501 ccaacgccta tccatcgccc gggcgctggt gaagaacccg tccatactga tactcgacga
     4561 tgccaccagc gcagtcgata cccgcactga agtagcattg cgccaggcac tccgcgaaga
     4621 gcggcgggcg gcgacgacaa tcattatagc ccagcgcgtg accagcgtga tgggtgcaga
     4681 ccggatactg gtgctcgaaa acgggcgggt ctccgccata ggccgccacg aggagttgct
     4741 tgagtcctgc cccgcgtatc gcgaactgtg ccgacttcag ctgggaacgg aggcggtgtg
     4801 agatgagcgg gagcacacct agggaaggct ctgatcggcg ccccggcgaa tttggcccgc
     4861 aagtaggctc ccagctaggc cccaggtttg gttcccgagc cagccgcctc ggacctgtat
     4921 cgaaacctaa ggacattggg gccgtcctgg ctcacctgtg ggtttacctg aggcagcaac
     4981 gacgcactct ggcgctggtg tttgcggcta cattagtcag ctcgctagta tcgatatttg
     5041 ggcctcgtct tattggaacc gccattgatc cgtacgtcct ggacggcgat cttctagggc
     5101 tgagccggat actcatcctc ctttctgcga tgtacgcgtc gagctctctc gcgtcttggg
     5161 tccagcagac ctgcatggtg gggttgtcga accgagccct ccgcgagatg cggcacgacc
     5221 tgttcgccaa ggtacaggca cttcccctgt cttttttcga cagcgcaacc catggcgatc
     5281 tcatgagccg gctcgccaac gacgtcgata ctgtgggcat ggcgctaggc cagcccatcg
     5341 ttcagctctt ttcgtcggtg gtaagcatct cgggaacgct gatcattatg ttctcgatga
     5401 gcgtgtggat ggcactgact gtgctggcaa tggtcccggt gactttgcta gtcaccacca
     5461 aggtggcggg ctggtcaagg aagctctaca gacgccgtca ggaggctctg ggcgagatca
     5521 acggatacat cgaagagatg gtgacaggcg cccgggtcgt caaggtattc ggccgtgagg
     5581 acgacgcatg cgccgcattc aatgacaaga gcgagcggtt acgggacatc gcctacagga
     5641 ctgaactcgt gtccggcatg ttcatgccag ctatgcacct gctcgacaat ttgagtttcg
     5701 cagtggtggc tctcattgga gcactgctgg cagcgaacgg gatcattcat ctcgggatcg
     5761 tcgcgtcctt cataatatac tcgcgccagt tcatgcaacc cgtccgggat ttatcgaacc
     5821 agtggaacac ctttcagtcc ggcctggcag gcgcggagcg cgtgttcgag atcatggata
     5881 ccgagtccga agccgacgcc ccgggcgcaa acgacctgcg tctgccggtt tcgggccacg
     5941 tcgagtttga acgcgtatcg tttggctata agccgggcgt gcctgtactg accgacatat
     6001 cactggaggc caaaccgggt cagaccatcg cgctggtggg cgccacaggc tcaggaaaga
     6061 caaccatcat cagcttgctg tcacgtttct acgacataga agaaggacac atatacgtag
     6121 acggcgtgga tgtccgggaa atccgcaggg aatccctgcg ccgcgctctc ggcattgttc
     6181 tgcaggactc ccagctcttt tcggggaccg tgcgggagaa catacgctac gggcgcccgg
     6241 acgcctccga cgccgatgtc gaaaaagccg cggagctggc gcttgcggat ccgttcattc
     6301 gtcgaatgcc tgaaggctac gattctgtgc tcaccgccga cggaggcaat ctcagccacg
     6361 gacagcgcca gttgctctgc atcgcccgcg cagtgctggc agacccggcc atactcatac
     6421 tcgacgaagc tacctcgagt gtggacaccc ggaccgaact gaggatacag caagccatcc
     6481 tccggttgat gcgtggccgc acgagtttcg tcatcgcaca ccgactgtcc accattcaag
     6541 gcgccgacca gatactcgtc atcgaccacg gccgcatagt cgagcgcggc gcccatgacg
     6601 agttgctggc caggggcggc gcatacgccc ggctccatga aagccagttc cacgtcgctg
     6661 cgcagcccgc aatgggtcat ggcatatttg agtcagtgta gcgacacaag ccgcggcact
     6721 tgctgagcag ggctttgcgc gaccgtatgg aaaatcgtac ggcctttcca ccagcctgat
     6781 ggaaaaccag acggttcggc acggccgggc caaatcgggg gaggattggg gatcgcaggg
     6841 ccctagggtg gcggttcgcc acggcctgct atcaaggcgc cgtgtcctta tgccggttct
     6901 agctgcgcat tcgataatca ctaatagtct ggaaaagagg agccctactg tcggttgcgt
     6961 tcggagaagc ccatgtgttc agaaccgttg ggtttcccat acggtctcgc aagaagcggt
     7021 gcggaaaacc aacaggtggt ggggctggct ggcaaacaca ggggaactgc aggtacagcc
     7081 cgcctttgcc agatgaactg ccatgattcg aggagactgc gaggtacttg aaggcaggat
     7141 ccgtgaggag cttgacaaca tcggatcatc cctgaagact gagggcggcg ttgtcagcat
     7201 tggcgtcggc gtcggcgccg gcgggcctat tcgcccctgc catgagcagc ccgagttcct
     7261 ccagcgagac gctgtcggcc tccacgacgg cgactatttt gccttcgtag atcacagcta
     7321 tcctatcaga caagttgagg atttcgtcta gttcctccga aatgaggagt atcccgcctc
     7381 ccgagtcgcg cacgtcgagc aggcgtcggt ggacgtactc ggtggccgcg atatccaggc
     7441 cgcgcgtggg atagcacgct agcaacacag caggcttccg cgttatgtcg cgggctagga
     7501 taaccttctg gatgttgcct cccgataggc tgccggcgcg cgtttctacg ctgggggtcc
     7561 tgacatcaaa cgtctccacg gtcttgcgcg accagttccg gatcacgtcg ttctgcagga
     7621 aaatccagcg ggcgaatgga ggcctatggt ggtctttgag aatcagattc tcctctaccg
     7681 aaaacgactg gatgatgccg aaactgagcc tatcttccgg tacgtagccg agaccggcgt
     7741 cgatcatcgc tgccggcgaa gcgccggtaa ggtcatggcc gcgcaggcgc actttgccgc
     7801 gactcacccg gcgaaggccg gtcatgatct cagccagttc tctctggcca ttgccagaca
     7861 ctccggcaag ccccaggatc tcaccttccc gcaccgcaag cgataggccc ttcagtgcag
     7921 ggaggccatt gtcggcgtcg gcccagactt ggtccagttc caacacgggt tgcccgtgcg
     7981 aggcttctcc agtgcgcttg gcagcttcaa tcttgcgtcc taccatcatc gtcgccaagg
     8041 actccgggct ggcctgtgag gtcggcacgg ttccgaccac agcgcctgcc cggagcactg
     8101 ttacgcggtc gcttaccgcc atgacttcgt tcatcttgtg gcttatgaag accagtgact
     8161 tcccggcctg ggccattccg cggagtatgg ctatgagctc gcccgcctcc tgcggcgtca
     8221 gcaccgcagt gggttcgtct aggatgagca actcggcgcc gcggtacagc gctttgatga
     8281 tctctacgcg ctgttgctcg cccaccgaca actgccatac gggcgcgtcg ggcctgattc
     8341 tgaaaccgta ttcgtcgccc agctccacaa tgcgctgtga cgcggtgtcg agatcgagaa
     8401 gggggcctct cgccgattgc aggccgagca cgacgttttc gaccacagtg aggctgggga
     8461 cgagcatgaa gtgctggtgt accatgccga tgcccagggc tatagcatca cgcggagagt
     8521 tgatcgtcac tgcctctccg ttgaccagta tctgcccgga gtcgggtcga tacagcccgt
     8581 atagcacttt catcaacgtg gtcttgcccg cgccgttctc gcctagaagc gcaagcacct
     8641 caccggagtt gatatcgagg tcaacgcaat cgttggcccg aaccccgggg aaggtcttcg
     8701 ttatctggcg catcttcacg tgtttgatgc gaggccccga tgtcgatccc atgaatatcg
     8761 cactcctcaa ccgcttgctg ggctactaga accgcctgct gagctactgc aaccgcttac
     8821 cggatattac ttgcctccag ccttgggcag ttccactttg agcttgccgg agatgatgtc
     8881 ttgcttggcc ttctctacgc ggtcaagcac ggccttgggt atcttgtttg ttagtttcgg
     8941 gttgaaacgc agctccaggg taccgtcggc aaaggtcagc gtaaggtgtt ttccgcccag
     9001 gattccctgg gcacgatagt tgatcatatc cttgacgact ttctcccagt ggtacacctg
     9061 cgccgcgaga acatgatcgg gcgcgaggga agcctggtcc atgtcggtgg acatccagta
     9121 cacgccggac ctttcggcca ctgcgcgagc ggctcccact gcctgctgag acgatcccgt
     9181 cagcacgtcg gctttggcgt ccatgtggac cttagctagc tcgccggcgc cgactatgtc
     9241 gccgaaagaa ccggtgtagg ctatgcgcac tttcacattg ggattccccg cagccacgcc
     9301 ttgctggaag ccgtagttat acttgatcgc atcacccgac tccaccgggc cgacgattcc
     9361 cacaacgcct gtctttgaca tcaaggcggc gaccatgccg agaagatacg cgccctcctg
     9421 ggcctggggg tcgtatgcga aaatgttggg cgcggtggtg aacccggttc cataggcgaa
     9481 agtggtcttg gggaagtcgg gagcaagttc catgagcacg ctctgatact gagcgccatg
     9541 agcgataatg atgtcgaatc cctggtcagc atattgccgg attgcggcgc ccgcgtccac
     9601 agggttgccc atccgttcgc tcgtaacaaa ctccagcttg tcggcgccca tctccttctg
     9661 gacagccttg agtccttcgt acatcgactg gctccatgcc atgtcgtcga tggtgctcgc
     9721 catgagcatg gcgatgcgga tcttcttagc gggggcgccg tagccgtagc ccgccccgaa
     9781 gaccatgagt gtcgccaaag ctagaaacgc tacgagaact atgcggtttt tcctcacagt
     9841 agacacctcc tgacgtattt gtgcgtcgac cgccgcggct gtcgacgacg acaccgcctg
     9901 atccgcccca acctcgatcg gctagccagc ttcccttcgg aacggcttgc cgagggcggt
     9961 tggggcccac actcgcctga cagctactgc cagagctgcg atggtcatga catatggcag
    10021 tatcactgcg aactcgtatg ggaaactgat gccgagcacc tgaatccata attggagtgc
    10081 gttcatcatg ctgaagagga gcgagccaag aaggatgccc acagggctcc acctgccgaa
    10141 atacaccagg gcgacggcga tgaaaccgcg gcctgcggtg atgttatcgg cgtacatgtt
    10201 agcctgacca acagtcagga aggccccggc aagcccggcc atggcgcctc ccagcaccac
    10261 acactgatat cggatggcgt tgacgttgac gcccaatgtg tcggcggctt gcggtgtggt
    10321 gcccactgcg cgcaccttga gcccccaggt tgtgcggtac agcacaaacc aggataccgg
    10381 aacgagcaac aacgagaagt agaccatcca gttgtgctgg aacagcacag ggccgagaaa
    10441 cgggatcttc gacagtaccg ggatgttcca tgggccgaaa cccttacatg atgtgatgaa
    10501 cccgagatac actcggaaaa gcagccctga caggccccag cagaacatgt gtaggccaat
    10561 gccggagatg ccttgttcgg cctgcatggt aacgctgatg aaggccatga gcaggcctaa
    10621 tgcagctccg actccgattg cggccagcac gccgagccac ggactgccgg tgcccagcga
    10681 ggtcatgaat cctgcgaagg ctcccatcat catgatgcct tcgagcccga ggttaaacac
    10741 cccggagcgc tgcgcgaaca tttcgcccag cgacgcatag aggaatggtg cagcgagcct
    10801 gatcccggta gcaagtatcc cgacgattat cccttggctt gagatagtgg ccatatcagt
    10861 tgctcacccc cttgccctgc cttgccatac cctgctttcc cgcaacgcct gctgagccgc
    10921 ctgacatccg gcggctacgg agtagatcgg gcctgcggat gagcaagtcg cttccgacca
    10981 cgaatagcac aactagcccc tgtatggcca tgacgatggc ggccggtatc tgcaccgccc
    11041 tctgcatcat gtcggcgccc agagtgagcg cgccgaagag tatggaagct gggatgcagc
    11101 ctaggggatg aaggcgcccg aacagcgcga ccacgattcc gctgaagccg taaccgccgg
    11161 agagcccttc gaggaccctg tgatgcacgc caagcacttc tacgattcca gccagccccg
    11221 cgaagcctcc cgcaaaggcc atggcaagta tttggtagtt ttcaaccctg attccagcgg
    11281 cttttgcagc ctgagggccg gcgcctgagg cgcgcatccg gtagccgatt gtggtgcgcc
    11341 aaaggaagat gtagaccagt atcgcgaagg cgatggcgat caccagtccc cagtgaaacc
    11401 gtgtgccgcg aatgatccgg ggaagccaga tcgatctggg cagcagagct gtttggggac
    11461 acccggtgcc gtaggccact tcctgtgggt cgatcatcgg accgcggatc aggtagaggt
    11521 tgagttggaa ggcgatttgg ttgagcatta cggtgctgag tatctcgttc accgaaagcc
    11581 gggccttgag ccagccggca attccgcccc ataatgcccc cgcgagcaca cccgcgatta
    11641 gcgtcatggg cagcagcact attgcaggcc aactcggaaa agccagggca acagctgcgc
    11701 ctgccagcga accggcgacc atctgcccct cgcccccgat gttgaggatt ccgctcctga
    11761 acgagatgac gattccgagg cctaccagga gaagtggtgt agcctttacc aggctttcgg
    11821 tgatgccgta tttgccgctg accggcccgg tgaacatcgc tttgtatgca gtgatgggat
    11881 tcgcgcgagc cagcaggatc agccccgctc caaccaagag tgccagaacc agagccccga
    11941 ccagcacgcc agcttgtaga agggagtcgg acgtgcccgt acgtttcttc atgacacctg
    12001 ctcctttaga taacacgcgt cgattccaat ggcagaagat atatcatatg aatagaggat
    12061 tctaggcaag gcacacttat cctgccttga gcacaactaa tagatacgaa ggaaatccat
    12121 tgtttacggc ccatgcaggt aatcctgctt tgtcgcgggg tcttgcactt gcacgggaga
    12181 gcatcgccgg cattcataca gaggcccagg aaatgcaagt tcaactatga tactcttatg
    12241 ctgagcgctg cgaggtttac ggtatcggca ggggccgagt ggattccaag acaggtgggt
    12301 cgtcgatgag ttctttggaa cgtagtatca gcatactcaa ggatgctttc ccggaggcat
    12361 tcaccgaggg aaggatcgat ttcgatattc tgcgcgggtt gcttggcgtt tgtgtggatg
    12421 atcggcacga gcgctatcgc ctcacctgga acggcaaggg ccgtgcccga cgcattgccc
    12481 aggccccttc ggtcggggcg ctccagccga gccctgagga gtcggtcgat tgggatacga
    12541 ctcagaatct cctgatcgaa ggggacaacc tggaggttct gaagctactg ctcaacgagt
    12601 atcaggggaa gatcaagatg atctacatcg accctccata caacacgggc aacgatttca
    12661 tataccgcga cgacttccgc gacagcgtcg caagctatct ccagctgacc ggacaggccg
    12721 acgctagtgg gcgcctgctg cgagccgacg cggaaacctc gggcaggtac catactgact
    12781 ggctgaacat gatgtacccg cgcctcaagc tcgcgcgcaa cttgctttgc gaggacggtg
    12841 ttatcttcat ctccatcggc gacaatgaag tggcgaacct caggattatc tgcgatgagt
    12901 tgttcgggga gggcagctgc ctaggctgtg tggctcgcgt ggccaagaag acgtcgaaca
    12961 agggcactca ctttgcgccg tccaaagact atgtgctggt ctacgcctgc agagccgaat
    13021 gtgtcgctcc tttcatggac gtagtagacc ctgcgtacgc ttcgcgtttc agggagcgcg
    13081 acgggcgagg tcggtacgcc acggtcggtc tgtaccaggc ggctctcgac ccgcgtccca
    13141 atcagcgata ctggatcgcc tgccccgacg ggtcgttcgc catccctccc ggcgaagtct
    13201 ttcctgatga agttgccgat ggggctcagg taaagccggc aagtcgccgc gaccgggtgt
    13261 ggcgctggag ccgcgagtcg taccttgcga agaagcatct tctggtcttc aagaagtccg
    13321 ctcgtagccc tctcgtgacg cccgacgggt cgccgtcgaa atggaatgtc tacaccaaat
    13381 actatcttga ggacagactg aatgacggga ttcgcccccg cgactacctt gacgacatca
    13441 ctaacgatct gggcacggcc gccctcaaga gcctggggct cgacgatttc ttcgagtttg
    13501 ccaaacctcc ggagctggtg cggcggctga tgcagtggct gggcggacgc gatggcattt
    13561 acctcgactt cttcgcaggt tcaggaacga cggcccaggc ggtcctggag cagaatgcgg
    13621 aggatggcgg cagccgtcgg ttcatcctgg ttcaattgcc tgagccctgc cctcatgact
    13681 cagtggcagc ccagaatggt tacaacacaa tcttcgacat ctgcaaagag cgcatccgac
    13741 gggtgatcgc acgcctgcgc gccgacgagg cgcatggcac cgcgctcgac ttgggatacc
    13801 gcgttttcaa gctgcgaggt gaagcagttg aagatcaagt tcgacccaga tcttgactat
    13861 cagcgcgatg cgatcgactc ggttgttcag atctttgcgg gtcaggaaga ctgccgggcc
    13921 gacttcgcgg gcgatctggt catcgggaac cgtctccgct tatcagagga agagatactg
    13981 aagaatgtga gggccattca gctttgcaat gggctagctc tgtctgatac ggtttccggc
    14041 atgaacttca ctgtggagat ggagacgggc accggcaaga catatgtcta cttgaggacc
    14101 attttcgagc tcaaccggga atacgggttt ctcaaattca taattgtcgt gccgtctatc
    14161 gcgatcaagg aaggggtcgc gaagtcgctc cagatggtcg aggagcatat gcgcgggctg
    14221 tatgataacg ttcggtttga ccacttcatc tacgatcccg ccagcctcag tcaggtgcgg
    14281 aacttcgcca cgagcgacta catacagatc atgatcatca acatcgatgc attccgcaag
    14341 agcttcgtcg acccagaaaa agagcatagg gccaacatca tccatcgttc gcacgaccgg
    14401 atgatgggcg ctcgcccaat cgagttcatc cagggcacga gcccgatcgt catcattgat
    14461 gaaccgcaga gcgtagatac gacggccaaa agcgctgagg ccatagcgtc gcttcatccg
    14521 ctgtgcaccc tgcggtactc cgccacccac gtggagaggc acaacatgat gtacaggttg
    14581 gatccggtcg atgcgtacca gcgcaagctg gtcaagcagg ttgaggtggc aagtgtcgag
    14641 gcaagccacg ggccgagcag gccgtatgtg aggctactgg gtgtagacaa cacaagaggt
    14701 tcgatcactg ccaggattga gctggatgcg ctgcacaggg gtggcgcggt caggcgcgtg
    14761 gccagatccg tccgctgcgg cgacgacctg caggaggtct cgggcagtcg ggatgtctac
    14821 accggctaca tcgtcgagaa tatctgctgt gagaggggta atgagcacat catgctcgcc
    14881 cgatggcctg aggctgtgcg gctgaaccag cctatcggtg atctagacga tggcgagtac
    14941 aagcgcattc agatacggac taccatccgg gagcacctgg acaaggaatt gatgctccgg
    15001 ccaaggggcg tcaaggtgct cagcttgttc tttatcgatc gggtcgccaa ctaccgttgg
    15061 tacgacgagg acggcaatcc gcgtaagggc aagtatgcgc agatgttcga ggaggagtac
    15121 gtaaaggcga tgggcgacgt taagtaccgt tcgctgccgg aaggggctga cccggcggct
    15181 gcggctgaga gcgtgcacaa cgggtatttc gccatcgaca ggagaaggga cgcggcaggc
    15241 cgggatatgt tcagggagtc gcggggcgat ggtgttgcgc aggccgacga gagcgcgtac
    15301 cagctgatca tgagggacaa ggagaagctg gcaagcttcg agtctgggct gaagttcatc
    15361 ttctctcact cagctctcag ggagggctgg gataatccca acgtgtttca gatctgcacg
    15421 ctgaacgaca cggtctctgc gatgaagaaa aggcaggaga tagggcgcgg tctgcgcctt
    15481 gccgtgaacc aggacggcga gcgggtttac ggcttcgatg tgaacacctt gactgtcgtg
    15541 gccaaccagt catatgcgga gttcgtccgg cagctgcaga gcgagatcga agaggataca
    15601 ggggtccgac tgggcgaagc gcgtcggctc aatgtcaaga acgcggccga acggaggcaa
    15661 gtcggaccgg gcgggtcgaa tggtgtcggc acaagcccag agccggggcc gaagcaggag
    15721 tgcaacactg ggtatcgtgt gacattcgat acagatgagc tgattaggcg gtgcgccggc
    15781 gaaatccggc ggatgccggc gatagatcac acgggaccta tgatacggaa agcgcgactc
    15841 gggatgggtc gcggcgggat cggggcagga gacgttcgcg tcagcgccga gatacatgag
    15901 gctcatggtc atccgttgcc cgacatagtg acccagttgc agaacgatac ctccctaacc
    15961 cggcgcacga ttgtgaaaat cctggtgcaa agtggaaggc ttgaggactt caaggccggc
    16021 ccgcaggtgt tcatagagaa ggtgtcggcg attatccggc gccgggtggg gctgtgcatt
    16081 caggagggcg ggacacatgc agggactgga tcctagtatc ggccaaagcc tggggtcgga
    16141 gagatacagc ttcacctgga acggcaagcg ccgcgccgga cttattgctc aagcgccgcc
    16201 cgacggcacg ctccgtcctt gccccgagca gtcagtcaat tgggatacga cgcgcaacct
    16261 cttcatcgaa ggtgacaacc tggaagtcct caagttgctg cagaagtctt atcacaagaa
    16321 agtgaagatg atctacatcg acccgcctta caatacgggt aaggattttg tgtaccgcga
    16381 tgacttcaca gacagtatcc aaaactactt gagattgacg gggcaggtgg atgaggcggg
    16441 gcgtcgcctc tcggccaacc ctgaaacatg cggccgctat catactaact ggctgaacat
    16501 gatgtatccc cggatgaggt tggcgagaaa cctcctgcga gacgacgggg tgatcgccat
    16561 ttccattgat gaccatgaac tggccaacct gcggaagatg tgtgacgagg tgttcggcga
    16621 ggagaatttc tgcggtgtgt tcatctggga gaagaagaag aagcctttgt ttctggacgc
    16681 caacatgggc actgtgaccg agtacataat ctgcttcgcc aaaaaccggg cacagtcgcc
    16741 gccctttggt gccggcgctg cccgggaggg gaagaagtac ccgttcaaca atgcgggcaa
    16801 cggcgttcag accctgacgt ttccggccgg gagtgtgcgg ttcggcttcg gtgatcaggt
    16861 ggtggcggct caggacatgt cggagggcag catcttcact gaactgctgg atgatgtggc
    16921 gattgtgggc gggcggaacg ccaatgcatt ccgcctgagg ggcgagtggc gatactccca
    16981 gcagaagctg gacgagtttg tgcaggaagg cgcagacatc gtcataagca agatcccgtt
    17041 caggccaaac tatgtcagcc gttcgggcga gctgaagaag acttcgaatc tgctgtcata
    17101 ccgcaccaac ggcgtgccca cgtatgagga tgcgacgcag gagatccgag cccttttcgg
    17161 ggccgatgtg atgagctacc ccaagcccag cgggttgatg aagtaccttg tacgtgctat
    17221 gacgggcgaa ggcgacatcg tcatggactt cttcgcggga tccgggagca ccgcacacgg
    17281 cgtcatgctc caaaacagcg aagatggcta caatcgccag tacatactgg tgcaactgcc
    17341 ggaacctctg aagcctgatg atgccgagag cgggccggcc ttcgagttct gccgggcaca
    17401 cggcttgagc cctacaatcg ccgaaatcgg aaaggagaga atccgacggg ccgccacggc
    17461 cgccgcggcg ggcgcggtgg agcggggggc ccttgggctt ggccctgagt ttggtcctgg
    17521 tcttggcgct ggccgtggct caggtcttga cctggggttt agagtcttca ggcttgacag
    17581 cagcaacctt tggccgtggg actcggaccc ccggaatctc gaggagtccc tgctgcaggc
    17641 ggtggagcgg atcaaggacg atcgacaaga ggcagacgtc ctatacgagt tggtgatcca
    17701 gtacgggctg gatctcgcgg cgtccgtcga aaggcgggag atcgacggca aggcagtcta
    17761 tatagtgggt gagggcgaac tggtggtatg ccttgccgat catatcagct cgagacttgc
    17821 tctcggcatt gcgcgactcc gcgcggagct tgcggcggcc cagatgcagg tggtgctcag
    17881 ggacgcaggc ttcgacagcg atgccgacag aatcaacacc atacaggttc tgcgcggggc
    17941 gggggttgaa agcgcaaaga cgctgtgatt cactggccat tgcgtccgag catatcctgc
    18001 tattgtcgac caactcctac cgaggtgtcc atgatgtgtc gacgagaggt gggtgaaaat
    18061 gcaggatttc tcggattccg caaagtacca gtgcgggcac ccgatggcca agatccttgc
    18121 cgaccactgt gatccttgcg gccgtcccgt tgccgtggaa ttctcatgcg gcgactgttc
    18181 caagaaggct cttaacgcca aggtaaagga tatttgcgcc gggttgctta tcctgacgcc
    18241 cttcgacgca gggggcatcg acatcaaggt attctgtgga ggcgcgctgg tagagagaga
    18301 gacggcgcag gtggtggtca ttcctctgga acgggtgtgc gctgtggagg tgggggctcg
    18361 cgaggtggac ggatgtcctc cgtgtcctcc ctgcccgccg ccatgttctg cgtgtccacc
    18421 atgtccaccg tgtccgcctt gccctccctg tccgccatgc ccttcgaacg ggcgacctcc
    18481 gacgaccggg ccggtgagca ctgggccgtc ctaacggctg aaggccgcgc ggagctccag
    18541 gtgtagttcg ggcttcgggc ggccttgaca atgtgcaggg cgctgtgact acatgcacat
    18601 attgtacaag ttgtacaagg tttttggtga gagatgtaga aacacagaca gtgtctgcag
    18661 cgcttctgcc tgtgggcaaa tcgggggtat cgagggtgcg cagtccagac aaggtttcat
    18721 ggccttcaca cgtgtatgtg ggaatactgg tgtgggcgtt gatcgtgccg gtgggctggg
    18781 ttgttttggg aagttccacg ccggcgctgg cgttttcgtt tgagacggcg tccattttct
    18841 ttgctgtgct cgctttcttt accgcatggc acacttacgg gtactctcgt tcgagctact
    18901 tgctgttcat aggcatgacg cagctgatag tggctttact cgactccttc agcctcatat
    18961 ccagtcccgt gctcaggctc agcattgaac tggggatcca gcatcagatg cagttttggg
    19021 ctgcggcgcg gacgatccac gctttttcca tgttcctggc gcccagatac gcccggcttc
    19081 gccgcccgag tattcgcatg ctggctggtt ccgcgttatt ggctattgcg ctggtagctg
    19141 cgatagtgtg gctgcccggg ttccctgagc tcgcgacggt ggatggcgcg ttgactcccg
    19201 acatggttgt gatcaactac cttatccttg gggtccttgc tctcagtgtg atcggccaga
    19261 ctctgtcgga cgtccgtgat cgggtgctgc gcgagctgat gaccgcgtca ttggcagtcg
    19321 ctgtggtgtc gcaagcctcg tttactgttg ccgggcctat caccaactac atgactgcag
    19381 gcgcgggcct tttcggccac attgcgaagg cgctatcata ctggcttgtg tatgaggccg
    19441 tggtcatcgg gggctatgag catcctcttg cccgcctgcg cgatggcctg agacacgttc
    19501 gggcgacgcg gcgggtcacg ttgtctagcg tgggcgacgg ggcgatcacc accaacaaag
    19561 acgggaaggt cacgctgctt aatcccgagg catcgaggat tacgggatgg acagagcacg
    19621 atgctttggg agtcgacatt gacagcgtgt ttcggctgtc gcttatgggg gcaggttcat
    19681 cggcggggcg gagatctcgc agcacttgtc tcaccgagtc ggttgtctat gccaaggatg
    19741 ccagcatcag atacgtggag tacacttcct cgccaatcca cgggccggac ggcggcgatc
    19801 tgggcgaagt catagtcttc agggagattt cggaacgtgt gcagaaagat gaactcatca
    19861 agacaagcat ggagaaatac cgtgcgctgt ttgagcacat gcccaatgga gtcgccgtgt
    19921 atcgctcagt tgacggaggc aaggatttcg tgttcgtgga gttcaaccgg gcaggcgaac
    19981 tcatcgaagg catcgatgcg gcgagcgttg tggggaggcg cctgactgag gtgttccccg
    20041 gattggcgaa aatgggcctg atggaagcgt tgcagagggt gtggcttaca ggacattcgg
    20101 aaaggctctc acctgcctac tgcgagaatg gacctcgctc cgggtggagg gagaactgga
    20161 tataccgcgt accgtcggga gaggtggtcg ccgttttcca ggacgtcacc gagcgtgtcc
    20221 aggccgaaca gagggttgag taccttaccg tccacgatgc cttgacaggc ctgtacaacc
    20281 gtcgtcacct ggaacaggtc gtcagccagt tggacgtaga ggctgtagtg ccggtgagtg
    20341 tggtgttcgg agacgccaac gggctcaagc ttgcaaacga tgccttcggc catgaggccg
    20401 gcgacaggct gctggtcaac atcgctaagg tgctgcaagc gtgtagtcgg agcgatgaca
    20461 tcatcgtgag gatgggcggc gatgagttcg tagtcatcat gcctggggtg cgtgagggtg
    20521 aagccgccga ggctgtcgtg cggatgaggc gcgccttcga tgaggctccc gcagatcctg
    20581 tgcgggtaag cttggccctg ggatcctcca ctcgctccac cccgtcggtc tctcttcgcg
    20641 aagtgcttgc cgacgctgag aagcacgctt accggcagaa actggccgaa agcaagagcg
    20701 cgcgggggtc tctcatgaaa tcgctcctta gaactttggc tgtgaagacg cctgagactg
    20761 aggatcatgt gttgcgcatg cgcgagcttg cgctggagct cgggcgcaac ctcggcttgc
    20821 ccgacagcac tctggacgac cttgcagtgt tgactctgat tcatgatatc ggcatggtgg
    20881 ggattccaga cagcatcctg acaaaggtcg acgatccgct ctctcatgat gagcaagagg
    20941 cgctgaagaa gcatccggaa atcggctaca gggtggcaca ggcctcaata gagctggccc
    21001 acgtggccga tggaatactg tcacaccacg agaactggga tggcacggga tatccgcagg
    21061 gcctcaaggg tgaggagatt ccgctgattg cacggattgt ggccatagtc gacgcgtacg
    21121 atagcatgac ggcaggccga agctacaagg cggctagatc taagaaggag gccattgatg
    21181 agatccggaa gcttgcggga gcgcgcttcg accccgatct ggcagaggcc ttcgccaggg
    21241 tcatcagcgg tcacgagggt tttcgcttcg gcacaattga acgatagcgg acttctgccg
    21301 acgatgggtg tgatgataag atagggtatc acactagttg aaggcggtgc tggtaggtgg
    21361 tacctgaccg gagtctcgcg tggcttgcga tactaacctt cgtagtcagc ttcagctccg
    21421 ggatgacagg cccggtcttt ccccttcacg cccaggctct gggcgccacg tatgagcaga
    21481 tcggcatggt atcggccgca ggttcgctga tccatgcggg cttcgcttcc cgtgctggcc
    21541 gacttgccga ccagctcggg cacgacctca tgttcctcct gtcttcgctg acaatagtcc
    21601 tttcgacgac ggcctacttc ttctcccgga cgctgctcat ggctgggctg ggcaaggccc
    21661 tggattcgtt gttcatagcg tcgtactggc cggctttgga gagcgcgtcg cactcgtcag
    21721 gcagcgccgc ggggcattcg ctcggcgtgg tgtacacggt ctaccctatg gccaccttcg
    21781 ccgccagcgc tgccactggc tggctcgcgg gtcggctggg ctaccgcgcg tctttcgctg
    21841 ctgcgctagt gggcggggtg gcggcggtgg tcggcgtatc aatcgggctg agcaggcgcc
    21901 gcagactcga cgcggggtat ggcgatgcga ccgttcgggt cggtcgccgg ctgcgcgcgc
    21961 gtggagagcg atcaaaaggg aacgtccgac tgctgcccgg ttcagggctg cggttcgccg
    22021 cggcgctcac cgcgtgtttc acgtactgcg tgcttgtggg agaggcgttt actttcgtgc
    22081 cgctgctggc ggaggcaaga gggcttcggg ttgaactggt cggcctgctg gtaggggtct
    22141 tctggctggg caggactgcc acaagtctgc ccgcgggcat gctggcggat cgcgtcggcg
    22201 ttgatgttgt gctcatacct gctttcatag tgggcggcat cggctcagcc gtagtggcat
    22261 ggcacgcggc gcccgttgtc atgtttgtcg gagtaacgat gatgggtttg tgcgctgggg
    22321 cggtagcgcc tgtagcgatg gttctggggg cacagtgtgt cgacccggga tcacacggct
    22381 gggccctcgg gctgtgtgaa accttctgtg gcattgggtt catctccacc gggcttgtgg
    22441 gaggcgtgct tgccgggcgg tgtgggctgg cagttccttt tgctgcaggg gcggcagtgt
    22501 gcactgctgt ggcgctgggg ctggctctgg cgtctaggcg cggcagacgg agctgacatg
    22561 ccggtccgtt ggcccagtgc aacacggcat tatgacgcga atgggagaac cctgtccgaa
    22621 cttgcgtcat acgatgcttc tagggacagt tagctcccca taatgccatg agaaggtggg
    22681 cagtacagtg aacggatgga tgtgtcctac tagcccgtac ggcggatacg gagcgtaccc
    22741 ctacaggcgc tggcgcgatc ctgaaggcga gtacgagtgg gaagagcgcc atatgctgcg
    22801 cgagatctac cggggtatgc atcatctgct gagggaagag gccgaagagg cccacaagat
    22861 cgacgagata taccatttgt gccacgatat tgcgaggatg gtggccggcc aaagacccat
    22921 ggagatgccg gagacggtca tgcctccgat gccgatggtc ccaccgatgg cgccgtcacc
    22981 tgtggtcgcg cctggtccga tgtatccgtg ccccagcagg cctccaatgg gagtcatcta
    23041 gcggatcaag gcgacgcgac acgattcgac gagactggac gagacagggc ggggcggggc
    23101 cccgcctttt gttctatctg atgtcaggaa gttggcgtgt cctacataat gctcgctcta
    23161 tgtcagtata attgcgtgag gttgaattta ctaagccgag aaggattccg caatcttgtg
    23221 tggaatatct actagaatat gatggggtgc cgcacagctc catcgtggac aacgacagat
    23281 atgaactatg gatgacgaat ggacgtctgc ggaagggggc gcgttcatgt cttgatcgct
    23341 gaagtctgac gaaattcgcg gaatggaggg attaggaaat gatatcgagt gtggatatcg
    23401 tcaaacccct cgtcaatcag gcgctggcct atgttagcaa ggaccctgtc gcaaacttgc
    23461 ccaagattct gaagataggc gagaagatag cgggcaagga gagccacaag gcccaggttc
    23521 gcgatgtgac gagagtgctg acggaatccg acggcaactg gcgcgagctg gccatcaggc
    23581 ttctcactga aactcacccc aacataatga agtctattgg agtcaacttc ttcgtcaacg
    23641 ctagcctagt cggtatccct aagcagtaca agatctctga ggaactcggc gtgcaagtgc
    23701 cctacgctat tctcatggac ccgaccgaaa aatgcaatct aagatgtacc ggctgttggg
    23761 ccggcgacta ccagcgtgtt cgtgaactag actatgaagt gatggacagg gtctgccgcg
    23821 aagctgaaga actgggcatt tatcttatcg ttgtatccgg cggtgaaccc atggttgcaa
    23881 aggacaagct gctcaggctg gccgagtccc acccgaatca gttgttccat ccatttacca
    23941 acggtacgct gatagacgag gatttcgtgt cggaaatggt ccgattgggc aatatcgcgc
    24001 cggcgctctc agtggagggc ctggaacagg agaccgacaa taggcgtggc aagggcgtgt
    24061 acaagaagat catgcacgcc atggacttga tgcgcgaggc cgggtgcata ttcgggttct
    24121 ctgcctgtta tgcccggaac aacgtggacg tgatagccag cgacgagttc atcgacacca
    24181 tggtcgacaa gggctgcacg tttggctggt tcttcacgta cgtacctgta ggctcagagc
    24241 ctaatttgga cctgatggcg acgcctgaac agagggccaa gatgtatgat gctgtgcgga
    24301 gtttcagaaa caccaagccc atcttcctcg tggacttctg gaacgatggc gagttctctc
    24361 tcggatgcat agccggcggt cgcaggtacc tgcacatcaa tgcagccggc gatgtcgagc
    24421 cctgtgcgtt tatccattac gctaccgaca atatcaacga tgtcagcctc aagcaggcgc
    24481 tggcttcgcc gctgatgcgg gcataccaga agagacagcc tttcaacgag aacatgttga
    24541 ggccgtgccc gctcatcgac aatcctgaga agctggtgga gataatccaa gaatctggcg
    24601 cccgctcgac ccagcttggc gagcatgcgg tcgagcctga ggtactggcc gacaagatcc
    24661 agaaggaata cacccagcac tgggcagccc gtgcagacga gctgtggcag tccaacccgc
    24721 accccttcca cgacagagca tgtgagtttg cgaagaagga ggaagcggag aggagagaga
    24781 ggcaggccag cagggtgtag aacagtcgga gacccgaaac agacgagaca gacgaaacga
    24841 gagcccgtgc agacgcgcgg gctcttgttt aggtgcaccc ggtgtcacgt cgaacttgcg
    24901 atgatgccac cgccgaggag catgtctccg gaatagaaca ctccggactg acccggggtt
    24961 atggcgcgct ggggcgaagc gaacttcacg cgaacattgc cgtctgcggc cggcgtcaac
    25021 agggctttcg cgggcgccga tttgtacctt atctgtacct gcgcctccat ctcgtcttcc
    25081 agcacatcga acgggatgaa gttcacctgt tcagcccgga actcggtgct gtaaagatac
    25141 tctcttggcc ccgcaacgat ggtattcgat tccggcctga tctcgatgac gtacatgggc
    25201 ccgtcatgtt tgctctgcag ttgccccagc ccacgccgct ggcccacggt atacattgcg
    25261 gttcccatat gctcgccgat gatcgtgccg tcaggagaca cgataggacc gggagcccct
    25321 agcgcagggc gttcgtggat gccaacaccg accgcagctg cagctttggc gaag
//
LOCUS       DAOTAC010000002        22841 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_1858_length_22841_cov_4.993724, whole genome shotgun sequence.
ACCESSION   DAOTAC010000002 DAOTAC010000000
VERSION     DAOTAC010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 22841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22841
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1858_length_22841_cov_4.993724"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..2190
                     /gene="fusA"
                     /locus_tag="PLM74_00085"
     CDS             103..2190
                     /gene="fusA"
                     /locus_tag="PLM74_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HON41513.1"
                     /translation="MQGELPLENIRNIGIMAHIDAGKTTTTERILFYTGRVHRLGEVD
                     AGTATMDWMVQEQERGITITSAATTCEWRDCQINIIDTPGHVDFTVEVERALRVLDGA
                     VAIFDAVEGVQPQSETVWRQADRYEVPRIAFINKMDRVGANFDNAVAMMEEMLGATVA
                     PVQMPIGAEDQFRGMIDLINRKAVVYLDDLGLSRQVTEVPEDLQEKAEQARERLVERV
                     AENDDEAMELYVAGEEIPSEVLVAGLRRATLSAKLTPVLCGSSFRNKGVQSLLDAIVD
                     YLPSPVDMPPVVGVDLSQAKERVTRKPSDSEPFCALAFKIATDPYVGKLAYFRVYSGI
                     AKAGSVIYNASKGKRERLGRILRMHANHREELSVISTGDIAAAVGLRDTTTGDTLSDE
                     VSPLLLERMEFPEPVIDVAVEPRSKNDEDRLTMSLVKLAEEDPTFRIHTDPESGQTII
                     SGMGELHLEIIVDRLLREFNVQANVGRPQVAYRETVTDSVKAEGRFVRQTGGRGQYGH
                     VVLEVEPGLPGSGLVFENKIVGGAIPKEFIPAVEHGIREAAGTGTLAGYPVVDVAVAL
                     VDGSYHEVDSSEISFKIAGSLGFKEAMEAAKPALLEPIMKVEIVTPEQYVGDLIADMG
                     ARRGKVTSLENAGTNQMIRVQVPLAEMFGYATSLRSLSQGRANHTMHFLRYEQVPPHR
                     AEEIVRRYRGVLS"
     gene            2215..3417
                     /gene="tuf"
                     /locus_tag="PLM74_00090"
     CDS             2215..3417
                     /gene="tuf"
                     /locus_tag="PLM74_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012268992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HON41514.1"
                     /translation="MAKQKFERTKPHLNIGTIGHVDHGKTTLTSAITNVLAKAGLASF
                     MDFDKIDKAPEEKARGITISTSHVEYESPKRHYAHVDCPGHADYIKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLARQVGVPSIVVALNKVDMVDDPELLELVELEVRDLLSE
                     YEFPGDDIPVVPLSALKAGECGCGKQDCQWCGPIWKLIEACDEYFPEPARETDKPFLM
                     PIEDVFTITGRGTVVTGRVERGVVKVGEEVQIVGLRPDTKKTVVTGVEMFRKLLDQGQ
                     AGDNIGCLLRGIDRDEVERGQVLAKPGSITPHTKFTGQVYVLTKEEGGRHTPFFNNYR
                     PQFYFRTTDVTGSIQMPEGTEMVMPGDNTTMTITLITPIAMEPGLRFAIREGGHTVGA
                     GAVISIIE"
     gene            3620..3928
                     /gene="rpsJ"
                     /locus_tag="PLM74_00095"
     CDS             3620..3928
                     /gene="rpsJ"
                     /locus_tag="PLM74_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HON41515.1"
                     /translation="MASQRIRIKLKAFDHKILDQSAERIVDTAKRMGSEVSGPVPLPT
                     ERNVYTVLRSVHVDKDSREQFEMRTHKRLIDILEPTPKTIDALMRLDLPAGVDIEIKL
                     "
     gene            3951..4586
                     /gene="rplC"
                     /locus_tag="PLM74_00100"
     CDS             3951..4586
                     /gene="rplC"
                     /locus_tag="PLM74_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HON41516.1"
                     /translation="MGRAILGRKIGMTQLFESDGRVHPVTVIEAGPCYVVQKKSQARD
                     GYEAIQVGFGDMRERLATRASAGHYARSRVKPKKYLREFRVESSDSFELGQEIRVDQW
                     SSGDLVDVVGTSRGRGFAGTIKRWNFHRGPMSHGSMYHRRVGSLGATAPARVFKGRKM
                     PGRLGGERTTTLGLTVVRVDPERNLIMVKGAVPGARGGLVTIRDSVKAKKE"
     gene            4600..5214
                     /gene="rplD"
                     /locus_tag="PLM74_00105"
     CDS             4600..5214
                     /gene="rplD"
                     /locus_tag="PLM74_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010896318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HON41517.1"
                     /translation="MPKAPLFDVKGNRLGEVELPDAVFGIEPNEYAVHEAVTLQLASR
                     RSGTHDTKTISEVAGGGRKPWRQKGRARQGSRRSPLWRGGGVVFGPTPRKYGYSMPKK
                     VRRLALLSALSARAQGDAVFVVDSLELPEPSTKAMAALFDAIGVEKPLVVTVARDEIV
                     EKSTRNIPNAMALGVAGLNVYDVVNHQALIIAREAVAKAEEALA"
     gene            5214..5501
                     /gene="rplW"
                     /locus_tag="PLM74_00110"
     CDS             5214..5501
                     /gene="rplW"
                     /locus_tag="PLM74_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006791157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HON41518.1"
                     /translation="MVHPQDIIIKPIITEKSTAMGEDSKYVFKVKNDANKVEIAKAVE
                     ALFKVTVVSVNTITVPGKLKRQGRSQGLTPQWKKAIVTLKDGDRIPLFEGA"
     gene            5529..6359
                     /gene="rplB"
                     /locus_tag="PLM74_00115"
     CDS             5529..6359
                     /gene="rplB"
                     /locus_tag="PLM74_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HON41519.1"
                     /translation="MGIKAFKPTSPGRRQMTVTAFEEITRSKPEKSLVAPLRRSGGRN
                     AEGRTTSRFRGGGHKRAYRIIDFKRDKDGVPGRIASIEYDPNRSARIALVHYFDGEKR
                     YILAPLGLKVGDVIESGPNADIKPGNALPLINIPVGTVVHAIELTAGRGAQMARSAGA
                     SAQIMAREGDYVTLRLPSSEMRMVRAECRATIGQVGNVEHENVTIGKAGRKRWMGRRP
                     HNRGVAMNPVDHPHGGGEGRAPVGMKSPVTPWGVPTLGYRTRRAKASDRLIVRRRGSK
                     "
     gene            6391..6675
                     /gene="rpsS"
                     /locus_tag="PLM74_00120"
     CDS             6391..6675
                     /gene="rpsS"
                     /locus_tag="PLM74_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005398638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HON41520.1"
                     /translation="MGRSLKKGPYVYPALADKVEKLNQAGKKEIIKTWSRASMVVPDM
                     LGHTIAVHDGRRHVPIYIVEDMVGHRLGEFAPTRTFRGHRQSDKTTRMKK"
     gene            6693..7031
                     /gene="rplV"
                     /locus_tag="PLM74_00125"
     CDS             6693..7031
                     /gene="rplV"
                     /locus_tag="PLM74_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HON41521.1"
                     /translation="MEAKAVARHVRMSPRKVRRVVDMVRGKSVDEALTILKYAPQIAA
                     EPVSKVVASAAANAENNLDLTRDDLYIAQAFVDAGPSLKRVLPRQRGMADRILKRTSH
                     ITVVVREREG"
     gene            7035..7703
                     /gene="rpsC"
                     /locus_tag="PLM74_00130"
     CDS             7035..7703
                     /gene="rpsC"
                     /locus_tag="PLM74_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HON41522.1"
                     /translation="MGQKVNPIGLRLGINRTWDSTWYADKKKYGELLHEDLKLRKYIK
                     NRLYAAGISRVQIERAANRVRVTIHTARPGMVIGRGGAEAEALRRDLEKITGKQVSVN
                     ILEVKHPETDAQLIAENIASQLERRISFRRAMKQAMMRAERLGAQGIRMRTSGRLGGA
                     EIARTEGYSSGKLPLHTLRAAIDYGFAEAKTTYGKLGVKVWVYKGDVLPSRAGGVRRE
                     GGAQ"
     gene            7704..8138
                     /gene="rplP"
                     /locus_tag="PLM74_00135"
     CDS             7704..8138
                     /gene="rplP"
                     /locus_tag="PLM74_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012268993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HON41523.1"
                     /translation="MLMPKKVKYRKQQRGRMKGIATRGNQLSLGEFGLQAIEPGWVTS
                     RQIEAARIAMTRYTKRGGNVWIRIFPDKPVTKKPAETRMGSGKGAPEYWVSVVKPGRV
                     MFEIGGIPEEVAREALRLAGHKLPIKTKFISKEEAGGEADEG"
     gene            8128..8331
                     /gene="rpmC"
                     /locus_tag="PLM74_00140"
     CDS             8128..8331
                     /gene="rpmC"
                     /locus_tag="PLM74_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HON41524.1"
                     /translation="MKAKELKKMSPEELDRRLVGLKEELFNLRFQLATNQLENPHRIT
                     DVKHDIARVKTVIRERELGAVRR"
     gene            8347..8607
                     /gene="rpsQ"
                     /locus_tag="PLM74_00145"
     CDS             8347..8607
                     /gene="rpsQ"
                     /locus_tag="PLM74_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013290159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HON41525.1"
                     /translation="MESARTQRKERFGVVVSDAMDKTRVVALERTFKDTRYGKTVRRT
                     NKVKIHDENNESRLGDKVRIMETRPISREKRWRLVEIVQKAK"
     gene            8639..9007
                     /gene="rplN"
                     /locus_tag="PLM74_00150"
     CDS             8639..9007
                     /gene="rplN"
                     /locus_tag="PLM74_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HON41526.1"
                     /translation="MIQPQTMLNVADNTGAKKVMCFRVLGGSERRYANVGDIIIATVK
                     EATPGGVVKKGEIVRAVVVRTNKELRRPDGSYIRFDENAAVIIDAQNNPRGTRIFGPV
                     ARELRDRSFMKIISLAPEVL"
     gene            9047..9376
                     /gene="rplX"
                     /locus_tag="PLM74_00155"
     CDS             9047..9376
                     /gene="rplX"
                     /locus_tag="PLM74_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014451917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HON41527.1"
                     /translation="MTAVVKKVKKGDTVMVLTGKDSGRRGKVLRVLPSEQKVLVENLN
                     MVKRHTKPTNTNPQGGIVDRPSPMPVGKVMVVCGKCNKPTRVGMKAKESGKFVRVCRK
                     CGAGLDK"
     gene            9427..9978
                     /gene="rplE"
                     /locus_tag="PLM74_00160"
     CDS             9427..9978
                     /gene="rplE"
                     /locus_tag="PLM74_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HON41528.1"
                     /translation="MEKTPRLKEKYENEVVPALEQKFGYKNIMEVPKVHKVVVSMGVS
                     DAVQDPKTLDSAANDMALISGQKPIITRAKKSIAAFKLRAGVPIGCKVTLRGARMYYF
                     LDKLFNAALPRIRDFRGVSGDSFDGRGNYTLGLKEQLVFPEIEYDKVDKIRGMDITIV
                     TSARTDEEGLELLRLMGLPVRTE"
     gene            9991..10176
                     /locus_tag="PLM74_00165"
     CDS             9991..10176
                     /locus_tag="PLM74_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012939249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HON41529.1"
                     /translation="MARKGLVETWKRGPKYRVREYNRCSICGRPRAYMRKFKMCRICF
                     RTLASRGEIPGVTKASW"
     gene            10211..10609
                     /gene="rpsH"
                     /locus_tag="PLM74_00170"
     CDS             10211..10609
                     /gene="rpsH"
                     /locus_tag="PLM74_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HON41530.1"
                     /translation="MVLTDPIADMLTRIRNANTVNHEVVEIPASRIKEELARIMKNEG
                     YIRDFEIGRGEKQNVIKISMKYGPNRQKVIRGLKRISKPGLRVYVKKDEIPKVLNGLG
                     VAVISTSQGLMTDRQAREAGLGGEVICYMW"
     gene            10621..11169
                     /gene="rplF"
                     /locus_tag="PLM74_00175"
     CDS             10621..11169
                     /gene="rplF"
                     /locus_tag="PLM74_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015908141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HON41531.1"
                     /translation="MSRIGRKPVAVPHGVEVHLDGRHITVKGPKGTVARELPREVTVE
                     VAEGTINVSRPSDDRIARSMHGLGRTLIANMVDGVTRGFEKSLEIVGTGYRASKQGKN
                     LILQVGYSHPVEIVPAEGIEIEVPAPNKITVRGIDKELVGQTAANIRAVRQPEVYHGK
                     GIRYAGERVALKAGKTGKAGGK"
     gene            11205..11573
                     /gene="rplR"
                     /locus_tag="PLM74_00180"
     CDS             11205..11573
                     /gene="rplR"
                     /locus_tag="PLM74_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HON41532.1"
                     /translation="MYNRVDRKQALARRHMRIRKHVVGTPERPRLCVFRSHAHIYVQI
                     VDDTVGRTLVSVSTTEPEIRAAVGYGGNIEAAKQVGRVAAERALGKGISQVVFDRGGN
                     IYHGRVAALAEAARESGLEF"
     gene            11590..12090
                     /gene="rpsE"
                     /locus_tag="PLM74_00185"
     CDS             11590..12090
                     /gene="rpsE"
                     /locus_tag="PLM74_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HON41533.1"
                     /translation="MARMNLEGTDLKEKVVSINPVAKTTKGGRTRSFRALVVVGDEKG
                     RVGAGLGKAYEVSEAIKKAVDDAKKNMMDVPMVGTTVPHEVTATFGAGCVMLKPAGPG
                     TGVIAGGPVRAVLELAGYRDILTKSLGSNNPLNMVNATIEGLKSMRTAQDVAQIRGKS
                     VEELAK"
     gene            12102..12287
                     /gene="rpmD"
                     /locus_tag="PLM74_00190"
     CDS             12102..12287
                     /gene="rpmD"
                     /locus_tag="PLM74_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014194602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HON41534.1"
                     /translation="MAKTLAITWKKSAIGEPEDQRATVRSLGLHKLGQTVEHDDNPVI
                     RGMVHKVRHLVEVREID"
     gene            12301..12744
                     /gene="rplO"
                     /locus_tag="PLM74_00195"
     CDS             12301..12744
                     /gene="rplO"
                     /locus_tag="PLM74_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HON41535.1"
                     /translation="MRLQDLRPPFGAKKNVKRVGRGTGSGHGKTAGRGSKGQLSRSGG
                     GKGAGFEGGQTPIYRRLPKRGFNNKFKVEYAVVNVGDLERVFESGDEVDGEALKEKGL
                     VKGFGVRVKVLGDGEITKSLAVRAHKFSDSAVSKIKAAGGSAEVI"
     gene            12745..14007
                     /gene="secY"
                     /locus_tag="PLM74_00200"
     CDS             12745..14007
                     /gene="secY"
                     /locus_tag="PLM74_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HON41536.1"
                     /translation="MLGALINAWRVPDLRKKILYTALLLFIYRLGSFIPVPGVNTASF
                     VAEFQRLAGETGVGLLSLLDMFSGGGLSRATVFALNVGPYITASIIIELLAVVIPALE
                     ELKKEGPEGRKKMSQYTRYGAVALALIQGYSYSVMFGTAVRPGVLWKLLIVLSMTAGA
                     AFTMWLGDKISEKGIGNGISLIIFAGILSRFPAQLQGVVGMLKQGIGNIALTLVYGVM
                     GLALIVAVIATTEAQRKIPVQYPKRVVGRRVYGGQSTHMPIRLNQAGVIPVIFASSVL
                     AFPQTIGQFIPAISASVNRFIYNAWVYNILYVVLVILFTYFYTGITFNPVEVADNMKK
                     YGGFIPGFRPGRPTAEYLDRVLTRLTFVGAIFLAAIALLPNITGKLTSGSISFGGTGL
                     IIVVGVALDTMKQIEAQLIMRQYEGFLK"
     gene            14034..14684
                     /locus_tag="PLM74_00205"
     CDS             14034..14684
                     /locus_tag="PLM74_00205"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009740507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HON41537.1"
                     /translation="MRLVLMGPPGAGKGTQAVGLSQEYGVPHISTGDMFRAAVAAGTQ
                     LGLAARRYMDAGQLVPDEITLGIVRERLTQPDAARGFVLDGFPRTVPQASGLREVLRG
                     SNLQLDAVVLIDVPDAELVKRAVGRRVCRECGRVWHVEFNPPPGDTCSCGGELIQRDD
                     DRAETVVKRLGAYHEQTEPLKGYYSNLGLLLQVDGTGGPESVRKRIADALGRCNSR"
     gene            14681..15427
                     /gene="map"
                     /locus_tag="PLM74_00210"
     CDS             14681..15427
                     /gene="map"
                     /locus_tag="PLM74_00210"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HON41538.1"
                     /translation="MIILKTPDEIVRMRRAGALVGRVLEVLRGSVRPGISTARLDRIA
                     ENAIREGDGIPSFKGYRGFPASICVSVNDEIIHGIPGDRILVEGDFVSMDVGAIVDGY
                     QGDAAVTLGVGAVSPEAERLMRVTRESLAAGIANARPGLRLGDIGSAVQALVESNGFS
                     VVRDFVGHGIGRKMHEEPQIPNFGRPGEGIRLRAGMVLAIEPMVNAGGHEVRILEDGW
                     TACTMDGSLSAHFEHTVAVTLDGPEVLTLP"
     gene            15451..15750
                     /locus_tag="PLM74_00215"
     CDS             15451..15750
                     /locus_tag="PLM74_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing RNA-binding protein"
                     /protein_id="HON41539.1"
                     /translation="MSSDGPSLGRLVVSKMGRDKGRLYIVVGLSGDRYAYIADGVTHR
                     VSSPKRKNNAHLIALEQVDEGIRSRLASGLPLSDTQVVTALRVYRPHQRKEEHAQ"
     gene            15750..15968
                     /gene="infA"
                     /locus_tag="PLM74_00220"
     CDS             15750..15968
                     /gene="infA"
                     /locus_tag="PLM74_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013985292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HON41540.1"
                     /translation="MAKEDCVEVEGTVVEPLPNAMFRVELENGHKVLAHVSGKMRMKF
                     IRILPGDRVTIQLSPYDLTRGRIVWRYK"
     gene            15980..16093
                     /gene="rpmJ"
                     /locus_tag="PLM74_00225"
     CDS             15980..16093
                     /gene="rpmJ"
                     /locus_tag="PLM74_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002344960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HON41541.1"
                     /translation="MKVRPSVKKMCDKCKIVKRKGRVRVICENPTHKQRQG"
     gene            16109..16480
                     /gene="rpsM"
                     /locus_tag="PLM74_00230"
     CDS             16109..16480
                     /gene="rpsM"
                     /locus_tag="PLM74_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HON41542.1"
                     /translation="MARIAGVDLPRDKRIEIALTYIYGIGLTSSKQILSEIGIAPDTR
                     VRDLTEDEIAKLRETIDRSYKVEGDLHREEAQNAKRLMEIGSYRGLRHRRGLPVRGQR
                     TKTNARTRKGPKKTVGGRKKS"
     gene            16496..16885
                     /gene="rpsK"
                     /locus_tag="PLM74_00235"
     CDS             16496..16885
                     /gene="rpsK"
                     /locus_tag="PLM74_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019908251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HON41543.1"
                     /translation="MAKRQARPRRRERKNIESGVAHIKSSFNNTLITITDPSGAVVSW
                     SSSGAMGFKGSRKGTPFAAQQAADVAARAAMEHGMRTIEVLVKGPGGGREAAVRSLQA
                     AGLEVSMIKDVTPIPHNGCRPPKRRRV"
     gene            16911..17534
                     /gene="rpsD"
                     /locus_tag="PLM74_00240"
     CDS             16911..17534
                     /gene="rpsD"
                     /locus_tag="PLM74_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HON41544.1"
                     /translation="MSRYTGPTCRLCRREGTKLFLKGDRCYSEKCSVGRRAYPPGQHG
                     QGRKKASEYGTQLREKQKLRRIYGIHEAQFARYFDMAERRRGITGENLLAILETRIDN
                     VVYRLGMADSRAQARQLVRHGHFAVNGKKVSVPSYEMRPGDEIAVRTGSRNLIHFKGI
                     AEGRVPSVPAWLSLGEDRLSGRVLSVPTRDQVDLDIQEHLIVELYSK"
     gene            17572..18555
                     /locus_tag="PLM74_00245"
     CDS             17572..18555
                     /locus_tag="PLM74_00245"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HON41545.1"
                     /translation="MIESPRPRIDCQEDGDRYGRFVVEPLERGYGITLGNSLRRILLS
                     SLPGAAIASVRIDGVLHEFSTIPGIYEDVTDIILNLKGLLVKLHGDTPRVIRLNAEGP
                     GDVTAADFEADAEVEILNPELHIATLGTVEQVNPATGRQEETGARLSLEALVEPGRGY
                     MPAEKNKRPDQPIGVIPMDSIFTPVERVNYTVENARVGGVTDYDRLVLEVWTYGTMRP
                     KEAVSSAAGMMTDLLRLFVNLSQEEEPEEPEAQPVQDNRERVMEMTIEELDLSVRSYN
                     CLKRAGINSVEELTRKTEEDMMKVRNLGRKSLEEVKQKLETLGLSLRQPEE"
     gene            18592..18969
                     /gene="rplQ"
                     /locus_tag="PLM74_00250"
     CDS             18592..18969
                     /gene="rplQ"
                     /locus_tag="PLM74_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010498832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HON41546.1"
                     /translation="MRQGKLGRTASARRALFRSSVTNLFVHDRIITTESKAKEMRPIA
                     EKLITWAKKGDLHHRRLAAQYILEPEALQRLFDEIGPEYVERPGGYTRIVKLGPRRGD
                     AAPLAVIELVGRGVGEKKAKAKK"
     gene            18981..19841
                     /locus_tag="PLM74_00255"
     CDS             18981..19841
                     /locus_tag="PLM74_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041587365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HON41547.1"
                     /translation="MKPVIRVENVSYTYDGGGPGEVEALLDVSLEVAGGELVAVIGHN
                     GSGKSTLAKHMNAIFVPTRGDVYVLGMNTRDESRLWDIRQTVGMVFQNPDNQIVATTV
                     EEDVAFGPENLGVPPAEIRRRVDEALAIVDMTGYEKSAPHMLSGGQKQRVAIAGVIAM
                     RPKCIVLDEPTAMLDPVGRSEVLATVRRLAAEEGIAVVHITHFMNEAVLADRVIVMEG
                     GRIALEGTPREVFSQPATLRRLDLDVPQVTELAYELRAAGAPLPRCPLTVAELVEDIA
                     ALVGKGAGLS"
     gene            19838..20785
                     /locus_tag="PLM74_00260"
     CDS             19838..20785
                     /locus_tag="PLM74_00260"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HON41548.1"
                     /translation="MSDTIIEVRDVCYTYMRGTPLARQALANVNVSIERGELVAIIGR
                     TGSGKSTLIQHFNGLLKPDSGSVVVGGRDIWRKGVALRDIRRKVGLVFQYPEHQLFEE
                     TVAADIAFAPRNLAMSEAEVDEAVREAMRVVDLDYDALCARSPFELSGGQKRRVAIAG
                     VLAMRPEVLVLDEPTAGMDPRGREEVLAEIRQLHETHGYTIVLVSHSMEDVARFAERL
                     IVMDGGAVVMEGSPREVFRNREDLVRMGLGVPQVTGLMAELAARGLPYAEDVITVEEA
                     LHTLLPALLGAPSASASASASAGAGAGTRAGNGEGEVGE"
     gene            20782..21576
                     /locus_tag="PLM74_00265"
     CDS             20782..21576
                     /locus_tag="PLM74_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HON41549.1"
                     /translation="MNISSVAIGQYIPGESLVHRLDPRTKIALSLVVVVALFIVKSAW
                     AYAALGALIIGTVAAAHVPLKMVLRGLRPLLFVMVLTFGLHLFMDPGEPIFRIGKIVA
                     TDKGLAQGLITVARLSLLIMATSIVTLTTSPIAFTDGLESLLKPLSFVGVPAHELAMM
                     MTIALRFIPTLLEETDKIMKAQMARGADFETGNIAKRARAMIPILVPLFVSAFRRADE
                     LAMAMEARCYRGGQGRTRLRQLRLGSLDVAALVLVTVYAVAVVAVL"
     gene            21578..22525
                     /gene="truA"
                     /locus_tag="PLM74_00270"
     CDS             21578..22525
                     /gene="truA"
                     /locus_tag="PLM74_00270"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HON41550.1"
                     /translation="MRRIGLVLEYVGTEYQGFQVQAGEAGAATDEAIGGPGPETARAA
                     QSSGAGRAMGIGRTGRTVQGTVEAAIARITGEQCRITGAGRTDAGVHALGQVASFDTC
                     SRIPGDRFAPALNTALPPDVRVLRSFQAPDGFNARYDAIAKVYRYIIVPSRIVGGARP
                     GPEAQLDPGAQPGGAILAGRALLVRRPLDMQAMAQASGCLVGQHDFRAFASTGSSAKT
                     SVRTIRRLEVGPIRFEILGLDAVAVEIEADGFLYKMVRTIVGALLGVGTGRADVGSVE
                     TLLASGDRSKGPATAPPDGLYLVRVIYPEPVATLVSGAM"
BASE COUNT         5020 a         5951 c         7753 g         4117 t
ORIGIN      
        1 cgtgaagaca tgcacaagat ggccgaggcg aacaaggcgt ttgcgcacta caggtggtaa
       61 gcccgctgtc cgggctatcg gggcgggaag gagggaagag atatgcaagg tgagttgcct
      121 cttgagaata tcaggaacat agggataatg gcgcacatcg atgccggaaa aacgactact
      181 actgagcgta ttctcttcta taccggccgc gtccaccgtc tgggcgaggt cgatgccggc
      241 acggccacga tggactggat ggttcaggag caggaacggg gcatcaccat tacttcggcc
      301 gctacgactt gcgaatggcg cgactgtcag ataaacatca tcgacacgcc cgggcacgtt
      361 gacttcactg tggaggtcga aagggctctc agggtgctcg acggcgcggt agcgattttc
      421 gacgctgtcg agggtgtgca acctcagtcg gagactgtat ggcgtcaggc ggatcggtat
      481 gaggtacccc ggatcgcttt catcaacaag atggatcgag tcggagccaa cttcgacaat
      541 gccgttgcga tgatggagga aatgctgggc gccaccgtcg ctccggtcca gatgccaatc
      601 ggcgccgagg accagttccg cgggatgatc gacctcatca acaggaaggc cgttgtatac
      661 ctcgacgatc tggggctttc gaggcaggtc acggaagttc ccgaggatct gcaagaaaag
      721 gcggagcagg cgcgcgagcg gttggtcgag cgagttgccg agaatgatga tgaggccatg
      781 gagctgtatg tggcgggcga ggagatcccc agcgaagtgc tggtcgcggg cctgcgtagg
      841 gcgacgctgt cggcgaagct cacgccggtg ctttgcggct cgtctttcag gaacaaggga
      901 gttcagtcgc tgctagacgc gatcgttgac tacttgccgt cgccggttga catgcctccg
      961 gttgtggggg tagatctttc gcaagcgaag gaacgggtca caaggaagcc ctccgactcg
     1021 gaacctttct gcgctctggc gttcaagatc gctactgacc cgtacgttgg caagctggcg
     1081 tattttcggg tttactctgg aatcgccaaa gcgggcagtg tgatctacaa cgcctcgaag
     1141 ggcaagcgcg aaaggctggg ccgcatcctc aggatgcacg ccaatcaccg ggaggaactg
     1201 agcgtgatct ccacaggcga catagcggca gctgtggggc tgagagatac caccaccgga
     1261 gataccctga gcgacgaggt cagcccgttg cttctggagc gcatggagtt tcccgagccg
     1321 gtcatagatg ttgctgtgga gcctaggagc aagaatgatg aagaccgcct caccatgtcc
     1381 ttagtgaaac tggctgagga ggatcccaca ttccgcatcc acacagatcc ggagagcggc
     1441 cagacgatca tctccggcat gggtgagctt catcttgaga tcatcgtcga caggctgctc
     1501 agggagttca atgtacaggc caacgtgggc aggcctcagg tggcctacag ggagactgtc
     1561 actgattcgg tgaaggcaga gggcagattc gtgaggcaga caggcgggcg cggtcagtac
     1621 ggccacgtcg tgttggaagt cgagccaggg cttcctggat cgggcctggt gttcgaaaac
     1681 aagatcgtgg gcggcgcgat ccccaaagag ttcatacccg ctgtcgagca cggcatccgc
     1741 gaggcggccg gtaccggaac cctagccgga tatcctgtgg tcgacgtggc ggtggcactt
     1801 gtggatggtt catatcacga ggtggattcc tcagagatct ccttcaagat cgctggctca
     1861 ctcggcttca aggaggccat ggaggcggca aagcccgcac tcctcgagcc catcatgaag
     1921 gttgagatag tcacgcctga gcagtacgtg ggcgacctca tcgccgacat gggagcgcgg
     1981 cgcgggaagg tgacttccct ggagaacgcg gggacgaacc agatgattcg cgtacaggtt
     2041 ccacttgccg agatgtttgg gtatgccact tcgctgcggt cgctttcgca gggaagggcc
     2101 aaccacacaa tgcattttct caggtacgag caggtccccc cgcacagggc ggaggagatc
     2161 gtgcggcggt accggggagt tttgtcctga gaacacaagg gaggattgaa agaaatggcc
     2221 aagcagaagt ttgagaggac caagccccac ctcaacatag ggacgatcgg gcacgtcgac
     2281 catggcaaga ctactctcac atcagccatc accaacgtgc tcgccaaggc cgggcttgcc
     2341 agtttcatgg acttcgacaa gatcgataag gcgcctgagg agaaggcccg tggaatcacc
     2401 atctccacat cgcacgtgga gtatgagagc ccgaagaggc actatgccca cgtcgactgc
     2461 cctggccacg ccgactacat caagaacatg attaccggtg cggctcagat ggacggagcg
     2521 attctcgtgg tgtcggccgc cgacggcccg atgccccaga cccgtgagca catcctgctc
     2581 gcccgtcagg ttggcgtgcc ctcgatcgtg gttgccctga acaaggtgga catggtcgac
     2641 gaccccgagc tgctcgagct ggtcgagctt gaggtgcggg atctgctttc cgagtatgag
     2701 ttccccggcg atgatatccc agtcgtgccg ctatcggccc tgaaggccgg cgagtgcggg
     2761 tgcggcaagc aggattgcca gtggtgcgga ccgatctgga agcttatcga agcgtgcgac
     2821 gagtactttc cggagccggc gcgtgagact gacaagccgt tcctcatgcc tatcgaggac
     2881 gtgttcacca tcacggggcg cggcacggtc gtcaccggca gggtggagcg cggcgtcgtg
     2941 aaggtaggag aagaggttca gatcgtgggg ctcaggcctg ataccaagaa gacggtcgta
     3001 accggcgttg agatgttccg gaagctgctc gaccagggcc aggccggcga caacatcggc
     3061 tgcctgctcc gcggcatcga ccgcgatgag gtggagcgcg gccaggtgct ggcgaaaccc
     3121 gggtccatta cgccgcacac caagttcact gggcaggtat acgtgctgac gaaggaagag
     3181 ggcgggcggc atacaccgtt cttcaacaac tataggccgc agttctactt caggactacc
     3241 gacgtcaccg gcagcattca gatgcctgag ggcaccgaga tggtgatgcc cggcgacaac
     3301 accacgatga cgatcaccct gatcactccg atcgccatgg agcccggtct gcgcttcgcc
     3361 attcgcgagg gcggccacac tgttggcgct ggagcggtca tatcgattat tgagtagctg
     3421 attgatccgc gtgccttacg aggccgcgac ccgcgggggc gggtcgtccc gcccccgctt
     3481 gaacggcaag gcgccgacat gcaaagcgcg agtacgtaag cgatgaagcg ggagattgtc
     3541 ctggccaatc ccgatggcgg gagtaaactt ccgtggagta tgtccgacaa agcaatcggg
     3601 cgatcaggag ggatattgcg tggcgagtca gagaatccgg ataaagctta aggcttttga
     3661 ccacaagatc ttggaccagt cagctgaacg catagtcgac acggccaagc gaatggggtc
     3721 cgaggtgtcg ggcccggtgc cgcttccgac agagaggaac gtctatacgg ttctccggtc
     3781 ggttcacgtt gacaaagact ccagagagca gttcgaaatg aggacgcaca agaggctcat
     3841 cgacatccta gagcccactc cgaagactat cgacgctctt atgcgcctgg acctgcctgc
     3901 tggagtggac atagagatca agctctagat gacgatgggg ggtgtaaggc atgggaagag
     3961 ccatactcgg cagaaagatt gggatgactc agctgttcga aagtgacggc agggtacatc
     4021 cagtcaccgt tatagaggcc gggccttgct acgtggtgca gaagaagagc caggcccgag
     4081 atggctacga ggccatccaa gtcggtttcg gcgacatgcg cgaacggctg gccacccggg
     4141 ccagtgccgg gcattacgcg cgcagcaggg tgaagccgaa gaagtatctc agggagttcc
     4201 gggtggagtc gtccgattcc ttcgagcttg gtcaggagat ccgtgttgac cagtggtcgt
     4261 cgggtgacct cgtggatgtg gtcggcacct ctcgcggtcg cgggtttgcg ggcaccatca
     4321 agcgatggaa ctttcatcgg ggccccatga gccatggttc catgtaccat cggcgtgtag
     4381 gatcacttgg ggcaaccgcg ccggccagag tgttcaaagg ccgcaagatg ccgggcaggt
     4441 tgggcgggga gcggacaact accctgggcc tgaccgtggt aagggtggat ccggagcgga
     4501 acttgatcat ggtgaagggc gctgtgcccg gagccagggg cgggcttgtg acgatccggg
     4561 actccgtgaa ggctaagaag gagtaaggag gcgtaggaga tgcctaaagc accactgttc
     4621 gatgtaaaag gcaataggct cggagaggtg gagcttcctg acgccgtctt cggaatagag
     4681 cccaacgagt acgcggtaca cgaggctgtc acgcttcagc tcgccagcag aaggtcgggc
     4741 actcacgata cgaagaccat ctcagaggtg gcgggcggcg ggcgcaagcc gtggcgtcag
     4801 aagggcagag cgagacaagg ttcgcgcagg tctccgctgt ggcgcggggg tggcgtggtg
     4861 ttcgggccca ccccgaggaa gtacggctat tcgatgccga agaaggtccg acggctagcc
     4921 ctgctctcgg cgttgtcggc gagagcccag ggggatgcgg tattcgtagt ggattcgctc
     4981 gagcttccgg agcccagcac aaaggcgatg gcggcgctgt ttgacgccat tggagtggag
     5041 aagccgctgg tagtgacggt cgcgagagac gagatcgtgg agaagtccac caggaacatt
     5101 ccgaatgcaa tggctctggg cgtcgccggg cttaacgtgt atgatgtggt gaaccatcaa
     5161 gcgcttatca tagcgcgcga agcggtcgcc aaagcagagg aggccttggc gtaatggtac
     5221 accctcagga tatcatcata aagccgatca taactgagaa gagcacagct atgggcgaag
     5281 acagcaagta cgtcttcaag gtgaagaacg atgccaacaa ggttgaaatc gccaaggccg
     5341 tcgaggccct gttcaaggtg actgtggttt ctgtcaatac gatcacagtg cccggcaagc
     5401 tcaagaggca ggggcggtcg caggggctga cgccccagtg gaaaaaggca atcgtcacgc
     5461 tgaaagacgg cgatcggatt ccgctgtttg agggcgccta aagcgacgca gcaaaggagg
     5521 tatgtgggat gggaatcaag gcatttaagc caacatcgcc ggggcgcagg caaatgacag
     5581 taactgcttt cgaggagatc acgcgctcca agcccgagaa gtccctcgtc gctccgctgc
     5641 gcaggtctgg agggcgcaat gcggagggcc ggactacatc gagatttcgt ggcggaggcc
     5701 acaagagggc atacaggatc atcgatttca agcgcgacaa ggatggagtg ccgggcagga
     5761 ttgcatcgat cgagtacgat cctaaccggt cggccagaat cgctctggta cactacttcg
     5821 acggggagaa gcgctacatt ctggcgcctt tggggctcaa ggtcggcgat gtgatcgagt
     5881 caggaccgaa cgccgacatc aagccgggca atgcgctgcc tctgatcaac attccggtgg
     5941 gcacagtggt gcacgccata gagctgacag ccggcagggg cgctcagatg gcgagatccg
     6001 ccggagctag cgcacagatc atggcgaggg aaggcgacta tgttactctg cgtctgcctt
     6061 catcggagat gagaatggtg cgcgccgagt gtagggcgac gataggtcag gttggcaatg
     6121 tcgagcacga gaacgtcacc atcggcaagg ccggacggaa gagatggatg ggcaggcgtc
     6181 ctcataaccg tggcgtggca atgaaccctg tggatcaccc gcacggcgga ggcgagggca
     6241 gggcgccggt aggaatgaag agcccggtga cgccgtgggg cgtgcctacc ctgggataca
     6301 ggacgcgcag ggcgaaggct tccgataggc tcatcgtccg gcgccggggt tcgaagtgag
     6361 caagggttct gaaaggaggc gcgccgattc atgggcaggt cgctgaagaa gggaccgtac
     6421 gtttacccgg ccctagctga taaggtggag aagctaaacc aggccggcaa gaaagagatt
     6481 atcaagacgt ggtcaagagc gtccatggtc gtcccggata tgctggggca cacaatagcc
     6541 gtgcatgatg gccgcaggca cgttccgatt tatatcgtgg aagacatggt cggccaccgg
     6601 ctcggagagt ttgcgccgac gcggacattc cgcgggcaca ggcagtccga caagaccacg
     6661 cgtatgaaga agtagaagga ggtcaggcac agttggaagc gaaagcagtt gcgcggcacg
     6721 tgcgcatgtc gccgcggaag gtccgcagag tagtcgacat ggtgcgcggc aagagcgttg
     6781 acgaggccct gaccattctg aaatacgcac cgcagatcgc agccgaacca gtgagcaagg
     6841 tggttgcgtc tgcggcggcg aatgccgaga acaacctgga tctgacgaga gacgacctct
     6901 acattgccca ggcatttgtg gatgcaggcc cttcgctcaa gagggtcctg ccaaggcagc
     6961 ggggcatggc cgacagaata ctgaagcgca cgagccacat cacggtcgtc gtcagagaga
     7021 gggagggtta gagcgtgggg cagaaggtca acccgattgg cttgaggctt gggatcaatc
     7081 gcacctggga ttccacgtgg tacgctgata agaagaagta cggcgagctt ctgcacgaag
     7141 acttgaagct caggaagtat atcaagaata ggctgtacgc cgcgggcata tccagggtcc
     7201 agatcgagcg ggctgccaac agagtgcgtg taaccattca cactgcacgg cccggaatgg
     7261 tcatcgggcg gggcggcgcc gaggcagagg cgctccgtcg cgaccttgag aagatcaccg
     7321 gcaagcaggt gtcggtgaac atcctcgagg taaagcaccc cgaaacagat gcgcagctca
     7381 tcgcggagaa catcgcgagc cagctcgaga ggagaatatc cttcaggcgg gccatgaaac
     7441 aggcgatgat gcgcgccgaa aggctcggag cgcagggcat ccggatgaga accagcggac
     7501 gcctgggagg cgctgagatc gcacgcacgg aagggtatag ctccggaaag ctccctcttc
     7561 atacgctcag ggccgccatc gactatggct tcgcggaggc caagacgacc tacgggaagc
     7621 tgggagtcaa ggtctgggtc tataagggtg atgttctccc atctagggcc ggcggagttc
     7681 ggcgggaggg aggggctcag taaatgttga tgcccaagaa ggtcaagtat cgaaagcagc
     7741 agagaggtcg aatgaagggc atcgctacgc gcggcaacca gctgtcgctg ggcgagttcg
     7801 gcttgcaggc gatagagccg ggctgggtga cctctcggca gatagaggcg gcgcgtattg
     7861 cgatgacgcg atacaccaag cgcggcggta atgtgtggat caggatattc cctgacaagc
     7921 ccgttaccaa gaagcctgcg gagacccgta tgggcagcgg aaaaggcgcg cccgagtact
     7981 gggtgtcggt ggtcaagccg ggcagggtca tgtttgagat cggcggcatt cctgaagaag
     8041 tcgcacgtga ggccctgagg ctggccgggc ataagctgcc catcaagacc aagtttatct
     8101 ccaaggaaga agcgggtggt gaagccgatg aaggctaagg agctcaagaa gatgtcgcct
     8161 gaggagctcg atcggaggct tgtaggcctc aaggaggagc tcttcaacct caggtttcag
     8221 ctcgccacga atcagctcga gaacccgcac agaataaccg atgtcaagca cgacatcgcc
     8281 agggtcaaga ctgtgataag ggagcgcgag ctaggcgccg taaggcgata gagaggaggc
     8341 caggttatgg aaagcgcgag aacccaacgc aaggagaggt tcggcgtcgt cgtgagcgat
     8401 gcgatggata agactcgcgt ggttgccctt gagcgcacct tcaaggacac ccgttacggg
     8461 aagacggtaa ggcgcacgaa caaggtgaag atccacgacg agaacaacga gagcaggctc
     8521 ggcgacaagg tccgcattat ggagacccgg ccgatctcac gcgagaagcg gtggcggctt
     8581 gtggagatag tgcagaaggc caagtaaacg gggccccgag cgaaaggagg aacactctgt
     8641 gattcagcct cagaccatgc tcaacgtggc tgacaataca ggcgccaaga aggtcatgtg
     8701 ctttcgagtg cttggcggca gcgaaaggcg ctacgctaac gtaggcgaca tcattatcgc
     8761 cacagtcaaa gaggctacgc ccggaggcgt tgttaagaag ggcgaaatcg tcagagctgt
     8821 tgttgtgcgg accaacaagg agctaaggcg gcccgatggc tcgtacatca ggtttgacga
     8881 gaacgctgcg gtcataatcg atgcccagaa caacccgcgt ggaacccgaa tcttcgggcc
     8941 cgtggcgcgc gagctcagag accgcagctt catgaagatc atctcactcg ctccagaggt
     9001 cctctaggcc tcgggcccat cccagaagta gggaggtgtg atcagagtga cggcagtagt
     9061 taagaaggtc aagaagggcg atacggttat ggttctcaca ggaaaagact ctggtcggcg
     9121 cggcaaggta cttcgtgtcc tgccgagcga acagaaggtc ctggtcgaga atttgaatat
     9181 ggttaagcgg cacaccaagc ccacgaatac gaacccgcag ggcggcatcg tcgacaggcc
     9241 gtctcccatg cctgtcggca aggtgatggt ggtgtgcggc aagtgcaaca agcctaccag
     9301 ggtaggaatg aaagccaagg agtccggcaa gttcgtgcgg gtttgcagga agtgcggggc
     9361 cgggctcgac aagtaggaca cggaacacag gtaggacagg gagaggcctg agagggggtc
     9421 aagtttatgg agaagactcc gcggctcaaa gagaagtacg agaatgaagt cgtgcctgcg
     9481 ctcgagcaga agttcggata caagaacatc atggaggtcc cgaaggttca caaggtcgtc
     9541 gtgagcatgg gcgtatctga cgccgttcag gaccccaaga cgctcgattc cgcggctaac
     9601 gatatggccc tgatcagcgg gcagaagccg attatcacgc gcgccaagaa gtcgattgca
     9661 gcgttcaagc tgcgtgcggg cgttcccatc ggctgcaagg ttactctgcg cggagcaagg
     9721 atgtattact tcctggataa gttgttcaat gcagctcttc ccaggattcg tgactttcgg
     9781 ggagtatcgg gcgattcctt cgacgggcgg ggcaactaca cgctcggcct aaaggaacag
     9841 cttgtgttcc cggaaatcga atacgacaag gtcgacaaga ttcgcggcat ggacattacc
     9901 attgtgacgt ccgcccgcac tgacgaggaa ggccttgagc tcctcaggct catgggtctc
     9961 cccgtgagaa ctgagtaagg agggggagaa atggcgcgca aagggcttgt ggagacgtgg
    10021 aagagagggc ctaagtacag agtcagagag tacaaccggt gctccatttg cgggaggccg
    10081 cgagcataca tgcggaagtt caagatgtgc aggatctgct tccgaaccct cgcttcaagg
    10141 ggagaaatcc ccggcgtgac caaggcgagt tggtgacggc agggtacccc acgagaggag
    10201 gtagaggacg atggtgttga ctgacccgat cgcggatatg cttacgcgaa tacgcaacgc
    10261 gaacaccgtg aatcatgagg tcgtcgaaat accggcgtct cggataaagg aagagctggc
    10321 ccggattatg aagaacgagg gctatatccg cgactttgag atcggtcggg gcgagaagca
    10381 gaacgtgatc aagatctcga tgaagtacgg gcccaatcgg cagaaggtta ttcgcggctt
    10441 gaagcgcatc agcaagccgg gcctgagagt ttatgtcaag aaggacgaga ttccgaaggt
    10501 actaaacggc ctgggagtgg ccgttatatc cacgtcgcag gggctgatga ctgaccgcca
    10561 ggcgagggaa gccggtcttg gcggcgaggt tatctgctac atgtggtagg tggtgtagct
    10621 atgtcaagaa tcggtaggaa gcctgtagca gtccctcatg gtgtggaagt ccatctggat
    10681 gggcgtcaca ttaccgtgaa gggccccaaa gggaccgtcg ctcgcgaact ccctcgagag
    10741 gtgacggtcg aggtggcaga gggtacgatc aatgtgagcc gtccgtccga cgatcggatc
    10801 gcccggtcca tgcacggtct gggcaggact ctgattgcca acatggtaga cggagtgacc
    10861 cgtggctttg aaaagtccct cgagatcgtg ggcaccgggt acagagcgtc gaagcagggc
    10921 aagaacttga tccttcaggt gggttactct cacccggtgg agatcgtgcc ggcggagggc
    10981 atcgaaatcg aagttccggc gcccaacaag atcacggtac gcggcatcga caaggaactg
    11041 gtggggcaga ccgcagcgaa tatacgcgcg gtcaggcagc ccgaggtgta tcatggcaaa
    11101 gggatcagat acgcgggcga acgcgtggcg ctaaaggctg gcaagaccgg caaggcgggc
    11161 ggcaagtaac tccgctctat gctcgatgaa aggagcgact cgtagtgtac aacagggttg
    11221 acaggaaaca ggccctcgcc cgccggcaca tgcgcatccg aaagcacgtt gtgggcacgc
    11281 ctgagcggcc gaggctctgc gtgttcagaa gccacgccca tatttacgtg cagatagtcg
    11341 acgacacggt gggccgcacc ctagtgtcgg tgtcgacgac tgagcccgag atccgggcgg
    11401 ctgtgggata cggcgggaat attgaggccg caaagcaagt aggtcgggtg gctgccgaga
    11461 gggcccttgg caaaggcata agccaggtcg tgttcgaccg cggcggaaac atataccatg
    11521 gcagagtggc ggcgctggcg gaagctgcgc gcgagtccgg cctagagttt tgacggggga
    11581 gggcagaaga tggcaaggat gaatcttgag ggaacggacc tcaaggagaa agtggtaagc
    11641 atcaaccccg tggccaagac gactaagggc ggccgcacca ggagcttccg cgcgttagtg
    11701 gtcgtgggag atgagaaagg ccgtgtcggg gctgggcttg gaaaggctta cgaggtgtcg
    11761 gaggcgatca agaaggctgt tgacgacgcc aagaagaaca tgatggacgt gcccatggtg
    11821 ggcactaccg ttccacacga ggtcaccgcg acattcggag ccggatgtgt tatgcttaag
    11881 cccgcgggcc ctggaacagg tgttattgcc ggcgggcccg tccgcgccgt attggagctt
    11941 gcgggttaca gggatatact gaccaagtct ctcgggtcga ataacccgtt gaacatggtc
    12001 aatgcgacga ttgagggcct gaagtccatg cgcacagcgc aggatgtggc ccagatacgc
    12061 ggcaagtccg ttgaggagct tgcgaagtag gaggggttcg gatggctaag acacttgcca
    12121 tcacgtggaa gaaaagcgcc atcggcgaac cggaagacca gagggcgaca gttcggtccc
    12181 tgggcctcca caagctgggc cagacggtcg agcatgatga caacccggtc atacggggca
    12241 tggtgcacaa agtgcgccac ctcgtggaag tccgggagat cgactgagag gggtgcaccg
    12301 atgaggctac aggatttgcg ccccccattc ggcgccaaga agaacgtcaa gagggtaggc
    12361 cggggcacgg gttccggtca cgggaagact gccggccgcg gcagcaaggg ccagctctca
    12421 cgatccggag ggggcaaggg cgccgggttt gagggcggtc agaccccaat ctacaggcgt
    12481 ttgccgaagc ggggtttcaa taacaagttc aaggtggagt acgctgtagt gaatgttggc
    12541 gaccttgagc gtgtattcga gtcgggcgat gaggtcgacg gtgaggcgct caaggagaag
    12601 ggtctcgtca agggatttgg tgtgcgtgta aaggtcttgg gggatggtga gataaccaag
    12661 tccctggccg ttcgcgccca caagttcagc gatagcgccg tctcaaagat caaggcagct
    12721 ggaggctccg cggaggtgat ataggtgctg ggagcgctga tcaacgcttg gagggtgccg
    12781 gatcttcgta agaagatcct gtacacggct ttgttgctgt tcatctacag gctcgggtcg
    12841 tttatacctg ttcctggagt gaatacggca agtttcgtgg cggagtttca gaggctggcc
    12901 ggcgagaccg gagtcggcct tctgagcctc ctcgacatgt tctctggcgg cggactgtct
    12961 agagcgacgg tatttgcgct gaacgtagga ccatacatca cggcttcgat catcatcgag
    13021 ctgctcgcgg tggtcatccc ggccctggaa gaactgaaga aggaagggcc ggagggccgc
    13081 aagaagatgt cgcagtacac caggtacgga gcagtcgcgc ttgcgcttat ccaggggtac
    13141 agctactctg tgatgttcgg tacggctgtg aggccgggcg tcttgtggaa gcttctcatt
    13201 gtgcttagca tgacggccgg ggccgcgttc acgatgtggc ttggcgacaa gatctcggag
    13261 aaaggaatcg gcaacggaat ctccttgatc atcttcgccg gcatactgtc gcgcttcccc
    13321 gcacagctgc agggcgtagt aggaatgttg aagcagggca taggcaacat agcgctcacc
    13381 ttggtatatg gcgttatggg cctagctctg atagtggcgg tcatcgccac caccgaagcg
    13441 cagcgcaaga taccggtcca gtaccccaag cgagttgtgg gccggcgcgt ttacggcggg
    13501 cagagcaccc acatgccgat acggctgaac caggccggcg tgataccggt gatattcgca
    13561 tcctcggttc ttgcgttccc gcagaccata ggccagttca ttccggccat atcagccagt
    13621 gtgaacaggt ttatctacaa tgcgtgggtg tataatatct tgtacgtcgt tttggtgatc
    13681 ctcttcacct acttctacac cggcataacg ttcaacccgg tggaagtggc cgacaacatg
    13741 aagaagtacg gagggttcat ccccggattt cggccgggca ggcccacggc cgagtatctc
    13801 gaccgtgtgt tgaccaggct caccttcgtg ggcgccatct tcctcgcggc tattgctctg
    13861 ttgccgaaca ttacgggtaa gctcacgtcg ggcagcatct ctttcggcgg caccgggctg
    13921 atcatcgtgg tgggcgttgc gctcgatacg atgaagcaga tagaggcgca gctgatcatg
    13981 aggcagtacg agggcttcct gaagtagaag tatgaagtgg gagtgcacgc atgatgcggt
    14041 tggttctcat ggggccgccc ggcgcgggca agggcaccca ggcggttggg ctttcgcagg
    14101 aatacggcgt cccgcatatc tctacgggcg acatgttcag ggcggctgtg gccgccggga
    14161 cccaattggg gctcgcggcg cgtcggtaca tggatgccgg ccagcttgtt cccgatgaga
    14221 tcaccctggg gatagtccgg gagaggttga cacagccaga tgcagctcgc gggtttgttc
    14281 tcgacggttt tccgcgaacg gttccgcagg caagcgggct gcgggaggtc ctgcgcggta
    14341 gcaaccttca gctggatgcg gtggttctta tcgacgtgcc cgacgccgag ctggtgaagc
    14401 gtgcggtggg gcgtcgggtg tgtcgcgagt gtgggcgggt atggcatgtg gagttcaacc
    14461 cccctcccgg cgacacatgt tcctgcggcg gcgagctgat tcagagagat gacgaccgtg
    14521 ccgaaacggt cgtgaagagg ctcggcgcgt accatgaaca gacggagccg ctcaagggtt
    14581 attactcgaa cttagggctg cttctgcagg tcgacgggac gggtggcccc gaatcggttc
    14641 gcaagcgcat cgcagatgcg ctcggaaggt gcaatagcag atgatcattc tgaagactcc
    14701 ggacgagatc gtcaggatga ggcgcgcggg cgcgctcgtc ggacgtgttc tggaagtact
    14761 ccgaggaagc gtgcggcctg gtatcagcac agcccggctc gacaggatag ccgaaaacgc
    14821 aatacgcgag ggggatggca tcccgtcatt taagggatat cgcggattcc ccgcctcgat
    14881 ctgcgtttcg gtcaacgatg agatcatcca cggtattccg ggagaccgca ttctggtcga
    14941 gggcgatttc gttagtatgg atgtgggcgc catagtcgat gggtaccaag gggatgctgc
    15001 cgtgacgctt ggcgttggtg cggtgtcgcc cgaggcagag cggctgatgc gggttaccag
    15061 ggagtcgctg gcggccggca tagcgaatgc gcggccgggc cttcgtcttg gagatattgg
    15121 cagcgctgtt caggcgcttg tagagagtaa cgggttttcc gtggtaagag acttcgtagg
    15181 gcacggaatc gggcggaaga tgcacgagga accccagatc ccgaatttcg ggcgcccggg
    15241 cgaggggatc aggcttaggg cagggatggt acttgccatc gaaccgatgg tcaatgcggg
    15301 tgggcacgag gttcggatac tcgaagatgg atggaccgcg tgcaccatgg acggatctct
    15361 gtcggcccat tttgagcata cggttgccgt gactctggat ggtcccgagg ttctgactct
    15421 cccgtaacac gcgaggaagg tgcggaggct gtgtcttccg atggtccgtc actcggacga
    15481 ttagtagtgt cgaaaatggg aagagacaaa ggaaggctct acattgtggt aggcctttca
    15541 ggagaccggt acgcatacat cgctgacggt gtcacgcata gggtgtcgag tccgaagcga
    15601 aagaacaatg ctcatctgat tgctctggag caggtggatg agggcatcag gtcgaggctc
    15661 gcatcgggcc tccctctatc cgatactcag gttgttacgg cgctgagagt ttacagaccc
    15721 caccagagaa aggaggagca tgctcagtaa tggccaagga agattgcgtc gaagtcgagg
    15781 gcacagtggt cgagcccctg ccaaacgcga tgttcagagt ggagctcgag aacggccata
    15841 aggtcctggc ccatgtgtcc ggcaaaatgc ggatgaagtt catcaggatt ctgcccggcg
    15901 atcgggtcac catccagctg tcgccttacg atctcacccg tggcaggatc gtgtggagat
    15961 acaagtagga gggtttgaca tgaaggtaag accgtccgtt aagaagatgt gcgacaaatg
    16021 caagatagtc aagagaaaag gccgtgtgcg ggttatatgc gaaaacccaa cgcacaagca
    16081 gcggcagggc taggaggtgt agcggcagat ggcgcgaatc gcaggcgtag accttccaag
    16141 ggacaagcgg atagagattg cccttaccta tatatatgga atcggcctga cgtcgtccaa
    16201 gcagatccta agcgagatcg ggatagcccc cgacacgcgc gtgagggacc ttactgagga
    16261 cgagatcgca aagctccgcg agacgatcga tcgcagctac aaggtggaag gcgacctcca
    16321 tcgcgaagag gcccagaacg ccaagaggct catggagatc gggagctacc ggggccttag
    16381 gcaccgtcgt ggccttcccg tgaggggcca gcggaccaag actaacgcac ggacccgcaa
    16441 agggcccaag aagacggttg gcggccgtaa gaagagctaa gggggaggat cgctgatggc
    16501 aaagagacag gcaaggccga ggaggcgcga acgcaagaac attgagtctg gcgtggcgca
    16561 tatcaagtcc agtttcaaca acacccttat tacgattacc gacccttcag gcgcagtcgt
    16621 ctcgtggtcg tcgtcgggcg ccatgggctt caaggggtcc cgaaagggca ctcccttcgc
    16681 agctcagcag gcagcggatg tggcggcgcg cgcggccatg gagcatggaa tgcgtaccat
    16741 cgaggttctg gttaaggggc cggggggagg acgcgaagcc gcggtcaggt cgctgcaggc
    16801 ggcagggctg gaggtcagca tgatcaagga tgtaacgccc atcccccaca acgggtgcag
    16861 gccgcccaag agacggcgcg tgtagccagg agcgaacagg aggtgctgaa atgtcgagat
    16921 atacgggacc tacgtgccgg ctctgccgtc gtgaagggac gaagcttttc cttaagggag
    16981 accggtgtta tagcgaaaag tgctctgtag gccggagggc gtacccgccc ggtcagcatg
    17041 ggcaggggcg caagaaggct tcagaatacg ggactcagct tcgggagaaa cagaagctgc
    17101 gaaggatata tgggatacac gaggctcagt tcgcacgcta cttcgacatg gcggagcgca
    17161 ggcgcggaat caccggcgag aacctgctcg ccatcttgga gacgcgcatc gacaacgtcg
    17221 tgtacaggct gggcatggcg gattccaggg ctcaggccag gcagttggtg cggcatggac
    17281 atttcgcggt gaacggcaag aaggtatccg tgccttcgta cgagatgcgg ccgggcgacg
    17341 agattgcggt gaggaccgga agccgtaact tgatccactt caaggggatc gccgaaggga
    17401 gagttcccag cgttccggcg tggctgtcgc ttggagaaga ccgactgagc ggcagagtgt
    17461 tgtcggttcc aacgcgagat caggtcgacc tggacatcca ggagcatctc atcgtcgagc
    17521 tgtactcgaa gtaatgctgc cccagatgag aaggagggtg ctgagcgcgc tatgatcgaa
    17581 tctccaaggc ctagaatcga ttgccaggaa gatggcgacc gatatggcag gtttgtcgta
    17641 gagccccttg agagaggata cggcataacc ctgggtaatt cactgcgcag gatccttctg
    17701 tcgtcgctac ctggggctgc gatcgcctcg gttaggatcg acggggtgct gcacgaattc
    17761 tccactatcc ctgggatcta cgaggatgtt accgatatca ttctgaacct caaaggcctt
    17821 ctcgtgaagc ttcacggtga cacccctagg gtcatcaggc tgaatgctga aggcccggga
    17881 gatgtgactg ccgccgactt tgaagccgac gcagaagtgg agatcctcaa tccagagttg
    17941 catatcgcca ctttgggcac tgtcgagcag gtcaacccgg caaccggcag gcaggaggag
    18001 accggcgcga ggctatcgct ggaggcgctg gtggagccgg gcaggggtta catgccggct
    18061 gagaagaaca agcgacccga ccagcccata ggcgtgatac cgatggattc catattcacg
    18121 cccgtcgagc gggtgaacta tacggtggaa aacgcgcgcg tgggcggcgt cactgactac
    18181 gacaggctcg tgctcgaagt gtggacctac ggcaccatga ggccgaaaga agcagtgagc
    18241 agcgcagccg gcatgatgac agaccttctg aggctattcg tcaatctctc acaggaggaa
    18301 gaacccgaag agcccgaggc gcagccggtc caggacaacc gggagcgcgt gatggaaatg
    18361 accatcgagg aacttgatct gtcggtgcga tcttacaact gcctcaagcg agccggcata
    18421 aacagcgtcg aggaactgac ccgcaagact gaagaagaca tgatgaaggt caggaatctc
    18481 ggccgcaaat ccctcgaaga agtcaagcag aagctggaga cgcttgggct gtcgcttcgg
    18541 cagcctgagg agtagtatta cagacggtta ggactaagga ggagagcgca agtgaggcaa
    18601 ggcaaactcg gccgcacggc gtctgcaagg cgcgcccttt tccgcagctc ggtgacgaac
    18661 ctgttcgtac atgacaggat catcaccact gagtcgaaag cgaaagagat gcgccccatt
    18721 gctgagaagc ttataacatg ggcaaaaaag ggcgaccttc atcatagacg tcttgcggcg
    18781 cagtacattc tggagccgga ggcgctgcag aggttgtttg atgagatcgg acccgagtac
    18841 gttgagagac ccggtggata cactcgcatt gtgaaactcg gacctaggcg gggagacgct
    18901 gcgcccctcg cagtgattga gctggtcggt agaggcgtcg gcgagaagaa agccaaggct
    18961 aagaagtgac gtgatagccc atgaagcctg tgattcgcgt cgagaacgtg tcatacacgt
    19021 atgacggcgg cggcccgggc gaagtggaag ctcttctcga cgtgagcctg gaagtcgcgg
    19081 gcggcgagtt agtggcggta atcgggcata acggctccgg aaagtccacc ctcgcaaaac
    19141 atatgaacgc cattttcgtg cccacacgcg gcgatgtcta tgtgctcggg atgaacacac
    19201 gtgatgaatc ccggctgtgg gacatccgcc agacggtggg catggtgttc cagaacccgg
    19261 acaaccagat agtggccact accgtcgagg aggatgtggc gttcgggccc gagaacctcg
    19321 gagtgccgcc tgccgagatc aggcggaggg tcgacgaggc tctcgcaatt gtcgatatga
    19381 ccggctacga gaagtccgct ccccacatgc tctcgggagg gcagaaacag cgcgttgcca
    19441 tcgccggcgt catcgcaatg aggccgaaat gcatagtcct cgatgagccc acagccatgc
    19501 ttgacccggt gggcagaagc gaagtgctgg ctaccgtgcg gcgcttagcg gcggaagagg
    19561 gcatagcggt cgtgcacatt actcacttca tgaacgaggc ggtcctggcc gacagggtca
    19621 tagtcatgga aggtgggcgc atcgcgctgg agggaacgcc gcgagaggtg ttttcacaac
    19681 cggcgacgtt gaggcgcctt gacctggacg ttccgcaggt aaccgagctc gcctacgaac
    19741 tcagggcggc cggggcgcct ctgccgcgat gcccgctcac agtggctgag ctggttgagg
    19801 atatcgcggc actcgtcggc aagggggccg ggctgagttg agcgacacga taatcgaggt
    19861 gcgcgacgtc tgctacacat acatgcgggg cacgcccctg gccagacagg cgcttgccaa
    19921 tgtgaacgtg tcaatagaac ggggcgaact ggtggccata atcgggcgga ccggttccgg
    19981 gaaatccacg ctcatacagc acttcaacgg gctgctcaag cccgattccg ggagcgtggt
    20041 ggtgggaggg cgcgacatat ggcgcaaggg cgttgcgctg cgcgatatta ggcgtaaagt
    20101 cgggctggta ttccagtatc cagaacacca gctgttcgag gagacggtgg ccgccgacat
    20161 cgccttcgcg ccacgcaacc tcgcgatgag tgaagccgag gtggacgagg ctgttcgcga
    20221 ggccatgcgc gtggtggacc tcgattatga tgccctgtgc gcgaggtctc ccttcgagct
    20281 ttcgggcggt cagaagcgta gagtagcgat cgccggcgta ctggctatgc gtcctgaggt
    20341 gctggtgctg gacgagccca ccgccgggat ggatccgcgt gggcgcgaag aagtgctggc
    20401 cgagatcagg caactccatg agacgcacgg ctacacaatc gtgctggtat cccacagcat
    20461 ggaggacgtg gctcgcttcg ccgagaggct aattgtcatg gacggtggcg ccgtcgtaat
    20521 ggagggctcg ccccgcgagg tgtttcgaaa tcgtgaagac ctcgtccgaa tgggcctagg
    20581 tgtgccccag gtcactggcc tgatggcgga gcttgcagcg agaggcttgc cctacgctga
    20641 agatgtgatc actgtggagg aagccttgca caccctgttg ccggcactgc ttggggcacc
    20701 gagtgcaagt gcgagtgcga gtgcgagtgc gggtgcgggt gcgggtacgc gcgcaggcaa
    20761 cggcgaaggg gaggtgggcg agtgaacatc tcttctgttg cgataggcca gtatatcccg
    20821 ggtgagtcac tcgtgcatcg gctggatccc cgcaccaaga tcgcgctgtc gctggtagtg
    20881 gtggtcgcgc tgttcatagt gaagagcgcg tgggcatacg cggcgctcgg ggcgctcatc
    20941 atcggaacag tggcggcagc gcacgtaccg ttgaaaatgg ttctcagggg actcaggcct
    21001 ctgctgtttg tcatggtgct cacattcggc cttcatctct tcatggatcc cggagagccg
    21061 attttccgaa taggcaagat tgttgcgacc gacaagggcc tggcccaggg gctcataact
    21121 gtggctcgtt tgtcgctgct cataatggcg acatcaattg ttaccctgac cacgtcgccg
    21181 attgcattca cggacgggct cgaaagcttg ctaaagccgc tctcattcgt gggtgtgccc
    21241 gctcatgagc tggccatgat gatgactatt gcgctccgct tcatcccgac tcttctggag
    21301 gagacggaca agatcatgaa ggcgcagatg gcgcgaggag cggactttga gaccggcaac
    21361 atcgccaaac gcgcccgggc aatgatcccg atacttgtgc ccctgttcgt cagcgcattc
    21421 agacgcgccg acgagctggc catggcgatg gaggccagat gttatcgggg cgggcagggc
    21481 aggaccaggc tgaggcagct caggctgggc tcgctggacg tggccgctct ggtgcttgtc
    21541 accgtgtatg ctgtagcggt tgtggcggtg ttgtgagatg cgccgtatcg gcctagtgct
    21601 agagtatgtg ggcacagaat accagggctt tcaggtacag gcgggcgagg cgggcgccgc
    21661 gacggatgag gccattggcg gtccgggccc agaaacagcg cgagcagctc agtcgtcagg
    21721 ggccggtcgg gcaatgggga tcgggcggac tgggcggact gtacagggca cggtagaagc
    21781 cgcgatcgcg cgcatcacgg gcgaacaatg caggataacc ggcgcgggac gcaccgatgc
    21841 cggagtgcac gccctgggcc aggtggccag cttcgacact tgttcgcgca taccgggcga
    21901 taggtttgcg cccgcgctca acaccgcgtt gccgccggat gtgagggtac tcagatcgtt
    21961 tcaggcgccg gacggcttca acgcacgata cgatgccatc gccaaggtgt atcggtatat
    22021 catcgtgccg agccggatcg tgggaggggc aagaccgggc ccggaggcgc agttggaccc
    22081 tggagcccag ccaggcggcg cgattttggc cggaagggct ttactggttc ggcgccctct
    22141 ggatatgcag gccatggcgc aggcatccgg gtgcctggtt gggcaacacg atttccgcgc
    22201 atttgcgtcg acaggttcgt ctgccaaaac cagcgtacgg acgatccgtc gcctggaggt
    22261 tgggcctatt cggttcgaga tcctggggct agacgcggtg gcggtggaga tcgaagcgga
    22321 tggattcctg tacaagatgg ttcgcaccat agtaggcgcc ttactcgggg tgggtacagg
    22381 ccgggccgac gtggggtctg tcgaaacact gctcgcctcg ggcgacagat ccaagggccc
    22441 cgcgactgct ccgcctgacg gactatatct ggtgagggtg atctaccccg agccagtggc
    22501 gacgctggtg tcaggggcaa tgtagaacta tggcggggct cgtacgttgc ctggcgagga
    22561 gccgatcccg gcgggatccc cgaacctgtg ccctgtgggc cagggtttcc gaggggcctg
    22621 ggcaaaccga cacaggatgg cccaaaaagg tgtcccgtag ggtacaggat gcccccgccg
    22681 accttggcgc gggcagacgg gccgagggcg atcgtttcgg cccacgcctt ctagctgcgg
    22741 tgttcgcacc tgcgcatttc actgcgcggc gcgtcgggcg agggcctccg cgaacctgtg
    22801 ccctgtgggc cagggtttcc gaggggcctg ggtaaaccga c
//
LOCUS       DAOTAC010000003        20923 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_2071_length_20923_cov_4.629624, whole genome shotgun sequence.
ACCESSION   DAOTAC010000003 DAOTAC010000000
VERSION     DAOTAC010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 20923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20923
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2071_length_20923_cov_4.629624"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="PLM74_00275"
     CDS             <1..685
                     /locus_tag="PLM74_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON41551.1"
                     /translation="AYGSVEAVHGVSLAVEQGQIATILGANGAGKTTTLRAISGLIRP
                     RSGSILLGDADISQIPAHEIVNRGVAHVPEGRRVFPTLTVEENLKLGAYKHRGHPDKV
                     SSNTARVYKLFPRLEERRMQLAGTLSGGEQQMLAIGRGLMPDPDILLLDEPSLGLAPK
                     LVQEIFRVVREINGQGVTILLVEQNARLALRISNHAYVLETGRIVLSGPADELRRDAR
                     VKKAYLGEK"
     gene            complement(781..2514)
                     /locus_tag="PLM74_00280"
     CDS             complement(781..2514)
                     /locus_tag="PLM74_00280"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HON41552.1"
                     /translation="MGSHDAAMQDRASVWMPLFVGLQAAFYLVILPRGMARLSAVLDA
                     SLPAMPLPAVSTAIGTIATVFGVYVAIRAFLVLSYVGKNWPGGHTLHIVDRHIYTFVR
                     HPLFWGYTLFWIGVGLWMRSPGRVILAAALGLAFAAWAKLVEEPRLASAFGERYEDYR
                     RSVPGLIPRWAALIADAEELDIVALLAVFLVRLLASFLWRIRAVGVENIPTEGPVVFA
                     SNHMSIADPYAIGLFVNRMIHYVTADEAFRSPVLRWFLRANRAIPKRKWGRDISAIRA
                     MRNHLKAGEAVGIFPQGQYNWDGGCNIVSDEVYRVLHYLGAPVVPITSLGAHECWPAW
                     SAGPAFCEWEVRFFPPVNPCDYESVSEFRREIESKMFSLVGHPPVPRRALSSHKGITM
                     VAWGCMSCGGAATLKEIKSGLECSKCGARWTVTRDLRIVDEKTGRSMTESEYRSALIQ
                     KLRAGEMEDAPDGVFNLSRTAHVWRLGPSSGVTDLGIGTLRLASGALTFSGPEGTAGV
                     EVPISDINFSFLDADAHLVVSEPGGAYELDIKDDSTLRWEDYLMAARGLTIRRWPTAD
                     EIRARSRERHR"
     gene            complement(2501..4333)
                     /locus_tag="PLM74_00285"
     CDS             complement(2501..4333)
                     /locus_tag="PLM74_00285"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HON41553.1"
                     /translation="MFNLKPNSAPNSSGIGSGAGNAARAMAPLALLAWAALFLAALPW
                     AMLHLAPRLSPYVPDTPLEMPRAIVGTCVCLLGAYVAWRGAVVLWFAGKGWPRSEPRY
                     LVSKCIYRFVLHPMYWGYTMFWIGLAIWTGSVALLAEALMLGVVFVSWSLAVEQPRLR
                     RRYGAAYEEYRRQTPMVLPVWKALYYDLRELPNTMLTLTMLGSALCRGLWNVRAEGEE
                     FIPKDGPVMVVCNHVNLADPLLLGMYFTRCIHFVASDELFRHPVTRWFFRVFGAMPKR
                     RWSRDIASIRSILRRLDSGDAVGIFPEGQRNWDGGPVIVGDEVYRLLHHLDVPILCAT
                     LIGGHEAWPRWSRLPGLSDVVVRFCEPIHPGQFSSVRDFRKAVEQRIFGFACEAPMPR
                     RALALHKGITSVLWGCIRCGGDMTLRETREGLECSKCGAAWAITPALELESRQSPERG
                     PRPQPGAQSGGQIRMLPREYHAALVERLYEGRMDGALGRDFRIGCSATGYRIESTRRL
                     KPLGQGTLKLSCAALVFEAGSGQAAGEERAEPLVIALDSIDFTYLNLANHLVVVGPHG
                     AVQFKVEGDSPVRWEDYVSAARGATIRQWKVREISYWRRKDGFS"
     gene            complement(4407..5471)
                     /locus_tag="PLM74_00290"
     CDS             complement(4407..5471)
                     /locus_tag="PLM74_00290"
                     /inference="COORDINATES: protein motif:HMM:NF025115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4163 domain-containing protein"
                     /protein_id="HON41554.1"
                     /translation="MLSRPSGKAILLLSFCMCLALALAQSQPALAFGPVGYHFTGSIG
                     GKFAVQMELQIEGAKVSGRYWYETIGTPLELAGTFADGRVRLTERTADGKTTGTWTGA
                     FNPLKSTWSGKWTSADGTKSFTFELAAAAEIVTTEEMRMGFIKVHVAHPVFTGAGKNR
                     GLQDVQAFVDREVWAAAASIWSDPADPMSDDLEFEPDASWWSWEHFSEVTTRYYSSSL
                     ISMMNEVYHFTGGAHGMTFHTSMNLKVGKNGVTAFAIDQIFKKGTGWEKVVSDYVIRD
                     LKARGADWVVSGEVREVPAKNMNRFTVSPAGIDLFFGPYEMGSYAQGTWVVLVPWSAC
                     KGLVDPNGPVAAIAGPAFGK"
     gene            6154..6987
                     /gene="nadE"
                     /locus_tag="PLM74_00295"
     CDS             6154..6987
                     /gene="nadE"
                     /locus_tag="PLM74_00295"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HON41555.1"
                     /translation="MDSGMNCEKVADALGAWIKERVQAAGCRGIVFGLSGGLDSAVVA
                     GLGMRAMPDGCLGVVMPCRSDPEDVKDALACARAFSLPALVCDLTSLFDQHLALVSGC
                     LSDAAARPDVAFAAPALTAMADSAARVAVSDPGRARMAQANLKPRLRMTALYYYANVL
                     GYLVAGTTNRSELTVGYFTKYGDGGSDIMPLAGLVKWQVRELASYLGVPRHVIEKAPT
                     AGLWSGQTDEGEMGISYAELDHYILTGEADDAVRRRVDSLNRAAQHKLTRPPVPSIPL
                     E"
     gene            complement(7090..8274)
                     /locus_tag="PLM74_00300"
     CDS             complement(7090..8274)
                     /locus_tag="PLM74_00300"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HON41556.1"
                     /translation="MLRDVMNIWRKEIMDTVRDRKGMTQAIVIPLVIGIFYASFTPML
                     MKAMTARREKPLTIPAQGVAYADARLLDMFGRYGITLEEYQGDLEAVIQDAKKESGLV
                     FEPGFTEDVAEERPAMARLLTNSTAGGIFGGGFSDTRLQLAVGAYNQMVVAERMAARE
                     VDPSILVPVALNTEDLATPEQRGAFFASFYLTILVAVTVAQGGLFVAIDVTAGEKERG
                     TLESLLVTPASDMAVFLGKLGAVFTISVIPLVLTLTAFWAVSNLMPQGAAGSEGLPGF
                     VLAGSLLTGIPLALVVSVAQMMISVRARTFRDAQSGATPLIFAVMIPGFVAAFSQAKA
                     LWACLIPVYGPFAALGRMAQAGGLQWDAVALSAVGSVIAAAVVLPVAVKMFTRETLLY
                     RS"
     gene            complement(8294..9118)
                     /locus_tag="PLM74_00305"
     CDS             complement(8294..9118)
                     /locus_tag="PLM74_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002621951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HON41557.1"
                     /translation="MIEVRDLVKHYGEVRAVDGVSFHCPDGHITGLLGPNGAGKTTTL
                     RMICGLIRPMQGTVIVDGNDVGRNPAAVRRALGVQTDSSGTYPRLTPREHFTYFGGFY
                     GLSGSDLDARVQAVIDMLGMGQFADRRTQGFSRGQRQKVILGRALVHDPKNVIMDEPT
                     SGLDVMAVRETRDVIRMLRDRGHCVLFTTHYMDEAAKLCDRVAIIIRGRIAAVGSPAE
                     LMGKTGRDSLEDAFVALAGGAQGLLVSEYANADGNAHGGGNSGSGNGLSSTGRRVR"
     gene            complement(9221..9592)
                     /locus_tag="PLM74_00310"
     CDS             complement(9221..9592)
                     /locus_tag="PLM74_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41558.1"
                     /translation="MRKTSVRRSRLSPAAYMAPAVGVLRRLAALFSAGAAAPRDYIEI
                     AIAGQTMIVERVMPAAAVAHELSVVLPHVEVRRTRDPSRPEAIQEEVVIESITVVSSP
                     VRRTADEVGGGIPGRGSKTKR"
     gene            9785..10849
                     /locus_tag="PLM74_00315"
     CDS             9785..10849
                     /locus_tag="PLM74_00315"
                     /inference="COORDINATES: protein motif:HMM:NF024948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HON41559.1"
                     /translation="MANSKVAFAMILALLCAGALFLVGLSLSSHSVPGYPDIHPLSAG
                     RVSVIAFSVWAFTAGLFVVAAVVFAIAERRESGTRSVLRLPRTQSLLTWLVLAMALLT
                     ALTLMNNGQARRELPAKEPAESSSPIGPEELSQPPPMPGAESSSEGKPSVRRAPTASS
                     PWAVYAIAMATLALAGVVAALALSPGRKPFAAAPEAAPEQAGDDHAIDPAWSDEEATC
                     AIQQEPDPRAAVIMCYRLFQSMLEQAGVRIEKHHTPEECGRIAVSSLKLPRRAVFRLV
                     GLYSRARFSEHYVSESHKSAALAEVRQIATYVREILAARDEAGDEAAAEVRDEARAEA
                     RAEAESKEVPDTVAFGPKRA"
     gene            10872..11321
                     /locus_tag="PLM74_00320"
     CDS             10872..11321
                     /locus_tag="PLM74_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41560.1"
                     /translation="MLLAMSVIIILSLDIYRLNPVWAKPFTIRLAGLSLIYVGYFRAW
                     QVTRASVEGPAPWNTRRRRHFWDWVDSMLPWRRKLEAPPRVCPEYDTLYRQLQMSMRR
                     GRYFARFLEEHGAPPHVVEYAQAHLADSSNLRHEQVSSVFDIMEESL"
     gene            11318..12313
                     /locus_tag="PLM74_00325"
     CDS             11318..12313
                     /locus_tag="PLM74_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013468178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HON41561.1"
                     /translation="MSVYTPEPADQSAPAIAWACDKCREVVSAVGTVIVGKDEQLELI
                     LTTLVSNGHLLLEDVPGVAKTLAAQATAQALGLDFRRIQFVPDLLPGDIMGSFIYDSR
                     ADSFRFRRGPIFTNLILADEINRGTPKTQSALLEAMQEQQVTVEGQSFALDKPFMVIA
                     TQNPLEFEGTYPLPEAQIDRFLARLSMGYPSPEAECEMLARRQSRQADAVILSPVLSK
                     QDFLEIQRAIETVHVNPDIHNYIVQIVTKTRVDRRVQLGASPRGSLALFKLSRSRAMM
                     QGRDFVTPDDVKAVAVPALAHRVLLRPELWAERISGETVVRDALDDVPVPKAEWR"
     gene            12320..13651
                     /locus_tag="PLM74_00330"
     CDS             12320..13651
                     /locus_tag="PLM74_00330"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HON41562.1"
                     /translation="MRTRVAGKAYVYIAMSGALVLAALATSRIELFMLATPLIFVLLS
                     LAFEAGAAEPKYTVSTHLQRDRCFEGDRTEVCVTVSAASCVPLLSLHIMVCDADNHVL
                     ASRHFTLSLGPGETRVFQFELEMPVRGVFYVGDVVSRAYTRSGLKSYPDRVWTGTECI
                     VYPRPDTIRIDWSASARSRQHVGESSSRMSGDGLEVSDVRLHMPGDPVRRINWRATQR
                     HGQLYINDSLRDRNIDAVVVVDGLVDLGEPPHSYLDCACRAAASIALAFLRSRDRVGV
                     IRYAGAVDWAVPRGGRNQLYAILDRLARVRTIQSFVSPNMRLIPRSVLPPGSLVLVIT
                     PLLDDRSIQMLAGLAARGCRPMVLYVSPMGLRPLTTTQAKPTDDAAALAERWWRLKHR
                     GKVAQLRSMGLMVAEWDGVSPLGACLSNHFGAPGFRPGGEAGGSSVRGNLL"
     gene            13648..14280
                     /locus_tag="PLM74_00335"
     CDS             13648..14280
                     /locus_tag="PLM74_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41563.1"
                     /translation="MKMTCTTLQRSWWTIAAPTAVCIMTTGYAALTAPTWWVVAVAFA
                     SAALCAAGAAAGDFGIVGWSGAAATGACAASLVENRPIHWGEPTLMCIGVLAMLEIGH
                     IVCIIRTREVNPSVFVHAHADADKDTPKDADNGNVDHNETLIALLPSVALRLAASGAA
                     SLGLTRLYLASAISGALRDSDTLFMMLLGAVAVGACMYWLVRISIQDFRR"
     gene            14351..16729
                     /locus_tag="PLM74_00340"
     CDS             14351..16729
                     /locus_tag="PLM74_00340"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HON41564.1"
                     /translation="MSVWGPVDGSCSGNSAACSDSLTSGTSSFISTLAGMTELEVFFL
                     SPDFVILFANDAAAKAAGVSPIDLIGHRIDGVLPMLYRRVEMFRPELERTGAVFVDQS
                     APEMQQGWLVLRDTYVVLMRNILEDSPLGWVVASLDPSLRTGLHNAVEMIPVPMAILQ
                     MPELSIEVANSRFTTLLAPSESLAATEPGHPGQAIAVMAGAAVRTGEYMKSSISWPGS
                     PDKGRRTDWTIYCCPLQRLGQNSRDSKAVVVLVQQTQARASMLRNLDVVRRLGSSLIG
                     SMSIPSFLHNAVSACASILSAPYCAIVQHDPLGNRLLRLAESSPLGLIRESAPVELYG
                     WLADIMQSKESSCRVIQLDKSPGWPEREQAGIEAFAVFPIAAHGRCYGHIVAALTDDG
                     SNLNSDDMHLAELTALYCDIALEQERAASDYAQLEAAQQKAEIEAAQTAKLLGALIDG
                     LEDGVIVVDAGHRVILANESAAALLGMSRDEMHNLDQVLARTQLAQMDGTPVPDQDRP
                     VRRLLQGLHVLRGDYVLTALGGSSRVLTFNGGVLRNPDGSISFAITVAHDRTELAQAE
                     KASRDYLRFVSHDLRSPLALISARAQMLERSPGDPDSVRKNAQAILRSVRQMNTMISD
                     LTDSVRLEFGSGLAVRPQALDLVALLNELIERWRETPEGDRIEACLPAGSMPPVCADP
                     DAIERILANLISNSLKYSPDEETIAVSATANDLEATISVADRGPGIRPEDAGRLFERF
                     FRSENARKHHDGLGLGLPISKALVEAQGGRIWIETELGRGSVFSFTLPLSLL"
     gene            16881..17084
                     /locus_tag="PLM74_00345"
     CDS             16881..17084
                     /locus_tag="PLM74_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41565.1"
                     /translation="MKRLRIPKSKLVLLAVVILVAVSAAMAGLAPAVAEAEESCVDCH
                     TNKDLLISLIPEREEEAAETGES"
     gene            17211..18158
                     /locus_tag="PLM74_00350"
     CDS             17211..18158
                     /locus_tag="PLM74_00350"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HON41566.1"
                     /translation="MAEAHVGVNASPSSLAEQNCTPCHDDVVATYGTSLHATLRGMEN
                     GLYSFTGEANRGKAKEAFDKYCYKCHATCGTCHVSKPEAANGGLIQGHKFFSSPDQDS
                     TCNACHGGRVANEFKGGIEGFPGDVHYEKLGFDCLTCHDGKSFHGDGSSPANRFEAPG
                     KPSCLDCHPDAAPGKGTVDAHNAHPEDTMDCYVCHATVNKSCYNCHVGEGAQSKLDFK
                     IGLNTRADWPYKYSVVRHMPGVSTMLDPFEPGLMDDYDSVPNWKTTMVHNIQRITPRN
                     KTCQSCHNNSKVFLTESRLVPGDSRASENVVVEQLPPKF"
     gene            18218..18664
                     /locus_tag="PLM74_00355"
     CDS             18218..18664
                     /locus_tag="PLM74_00355"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HON41567.1"
                     /translation="MKNTIRAAFAAMLILTLFAVPTFAAEDAVVKAVDNFVSNLPSDW
                     FQMKPEVLLQKLDLGEEIFVLDVRRPDEFAAGHVEGAVNIPVHDLAKHVAELPDDPDT
                     LIVVYCRSGVRSMFATSALLVLGYNKVYNMPGGFLAWQDAKYPVEK"
     gene            complement(18921..19586)
                     /locus_tag="PLM74_00360"
     CDS             complement(18921..19586)
                     /locus_tag="PLM74_00360"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013298543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HON41568.1"
                     /translation="MLYRLLRTALLETVWMVVVSGAISIVLGTLVGAALVFFSDPGLG
                     RDWRINRIFRIDQVLSAIVNVGRSLPFLVLMVAIIPLTRLLVGTSIGTQAAIVPLSIG
                     AIPFAARVVESSMRGVDRGVVEAALVMGASPLEVLFKVMFPEALPSLVLGFTLTLVSL
                     VSFSAMAGAVGGGGLGDLAIRYGYQRFRTDVMIATVVVLVVFVQAIQWVGQAISVAVD
                     RKR"
     gene            complement(19592..20329)
                     /locus_tag="PLM74_00365"
     CDS             complement(19592..20329)
                     /locus_tag="PLM74_00365"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HON41569.1"
                     /translation="MISLENVSKKYPGQSMPALSGVSFAVEQGDICGIVGLSGAGKST
                     LIRCMTGLERVDSGSIHIGDVDITKLAGAELRQAQRRLGVVFQHFNLLSHRTALGNVM
                     FPLEIARTPAREAKRRAEELLDVVGLADKRDAYPAKLSGGQQQRVGIARALATNPAVL
                     LCDEPTSALDPITTQSILELIRQVNADFGVTVLIVTHQLSITQQACGSLVVIDEGQIV
                     EKGPAQEVLRTPTSRILKAMVTTLSWS"
BASE COUNT         4305 a         6674 c         5977 g         3967 t
ORIGIN      
        1 agcttacgga agcgtcgaag cggtacacgg cgtcagcctg gccgtggagc aaggccaaat
       61 cgctacaatc ctgggcgcca acggcgccgg caagacaact accctgcgtg ccatatcagg
      121 cctcataagg ccgagatctg ggtccatcct actaggggat gccgacatat cccagatacc
      181 ggcacacgag atcgtaaacc gcggcgtcgc ccacgtgcct gagggcagac gcgtgtttcc
      241 aacacttacg gtggaggaaa acctgaagct aggcgcatac aagcacagag gacaccctga
      301 caaggtcagc tccaacacgg cccgggtgta caagctcttc cccaggcttg aggagcgccg
      361 catgcagctg gccggaacac tgtcaggcgg agagcagcag atgctcgcca ttgggcgcgg
      421 gctcatgccc gaccccgaca tcctgctgct agacgaacca tcgctgggcc ttgcgcccaa
      481 gctggtccag gagatattcc gagttgtccg cgagatcaac gggcaaggcg taaccatact
      541 ccttgtggag caaaacgccc ggctggccct gcgcatctca aaccacgcct acgtgctgga
      601 gacagggcga atcgtcctct ccggcccagc ggacgaattg cgccgcgacg caagggtcaa
      661 gaaagcctat ctcggagaga agtaagctca gcaaaacgga ctgagcaata cgaactgagc
      721 aacacggact aagcaactga cagcgggagg ccggccttaa gggctacgcc tcccgctgtg
      781 ctagcgatgc cgctcgcgcg acctcgctct gatctcgtcg gccgtgggcc acctgcgtat
      841 ggtcaagcct ctggctgcca taaggtagtc ttcccacctt agtgtggagt cgtccttgat
      901 gtcgagctcg taggcaccgc ccggttccga cacgacaagg tgagcatcgg catctaggaa
      961 ggagaagttg atatcggaga tcggcacttc cactccagcg gttccctccg gaccggagaa
     1021 tgtgagcgcg ccgctggcga gcctaagcgt tccgatgccg agatcagtca cgcccgatga
     1081 gggtccgagc ctccatacat gcgcagtgcg cgaaaggttg aacacgccat caggcgcgtc
     1141 ctccatctcg ccggcgcgca gcttctgaat cagggccgac ctgtattcgc tctccgtcat
     1201 gctccgcccg gttttctcat cgacgatcct gagatcgcgc gtcacagtcc atctggcgcc
     1261 gcacttggag cactccaacc ccgacttgat ctccttgagc gtcgcagctc ccccgcagct
     1321 catgcagccc catgccacca tggtaattcc tttgtgcgat gacagggcgc gccgcggaac
     1381 cggggggtgg cctacaaggg agaacatctt tgactctatc tcgcgccgaa actcggacac
     1441 cgactcatag tcacatgggt tcacaggcgg gaagaatcgg acctcccact cgcaaaaggc
     1501 aggcccggct gaccaggcag gccaacactc atgggcgccc agggacgtga ttggcacaac
     1561 cggcgcgccc agatagtgca gtactcggta cacctcatcg ctcacgatgt tgcagccgcc
     1621 gtcccagttg tactggccct gggggaatat tcccacagcc tctcctgcct tcagatggtt
     1681 gcgcatggcg cggatcgccg atatgtcgcg gccccatttg cgcttaggta tcgccctgtt
     1741 cgcgcggaga aaccatctca gaacaggact tctgaacgcc tcgtcggctg taacgtaatg
     1801 gatcatccta ttgacaaaca gaccgatggc gtacggatcg gcgatgctca tgtggttaga
     1861 tgcaaatacc acagggccct cggtggggat attctcaaca cccactgccc tgattcgcca
     1921 gagaaaggac gccagtaggc ggacgaggaa gactgccagc aaagccacga tgtcgagttc
     1981 ctccgcatcg gcgatcagcg ccgcccatcg cgggatgaga ccggggacgc ttctgcggta
     2041 atcctcatac cgctcaccaa aagcgctcgc cagacgtggt tcctctacga gcttagccca
     2101 cgcggcgaaa gcaagcccga gcgccgccgc aaggatgacg cggccgggcg agcgcatcca
     2161 cagcccgact ccgatccaga acagcgtgta gccccagaag agcggatggc ggacgaaagt
     2221 gtagatatgc ctgtctacga tgtgcaaggt gtggccgccc ggccagttct tgcccacgta
     2281 tgacaagacc aggaacgccc gaatcgccac atagacaccg aatactgtgg caatcgtgcc
     2341 aatggccgtg ctcactgcgg gcagcggcat cgcaggcagc gatgcatcca ggaccgctga
     2401 gagcctggcc atgcccctag gtagtatgac aaggtaaaag gcggcttgca gtccgacgaa
     2461 caacggcatc catacgctgg ccctatcctg catagcagca tcatgagaac ccatctttgc
     2521 gcctccaata gctgatctct ctgaccttcc actgcctgat ggttgcgccg cgcgctgccg
     2581 acacgtagtc ctcccagcgc accggcgaat caccttccac tttgaactgc accgcgccat
     2641 gcggtcccac aacgactagg tggttggcga ggtttaggta cgtaaagtcg atactatcca
     2701 gcgcaatcac aagtggctcc gcgcgttctt cgcctgcagc ttgtccgctc ccggcttcaa
     2761 agaccagcgc tgcacagcta agcttcaaag tcccttggcc cagcggcttc agccgccgag
     2821 tagactcgat tctgtagccc gtggcactac acccgatccg aaagtccctc cctagcgccc
     2881 cgtccatcct accttcgtag agacgttcga ccagcgctgc gtggtattct cggggaagca
     2941 tgcgtatctg cccgccgctc tgggctcccg gttggggcct cggcccccgt tcgggggatt
     3001 gcctagactc cagctccaga gcgggcgtaa tagcccaggc cgccccgcac ttcgaacact
     3061 ccagcccttc acgggtttcg cgaagggtca tatcgccgcc gcatcgaata cagccccaca
     3121 gtacgctggt aatccccttg tggagagcaa gtgcgcggcg aggcatcggc gcttcacacg
     3181 caaagccgaa tatcctttgc tcgacggcct tccggaagtc ccgcaccgac gaaaactgcc
     3241 cgggatggat aggttcacag aagcgaacaa ccacatcgga caacccgggc aacctcgacc
     3301 atcgcggcca ggcttcatgc ccgcctatca aagttgcgca gagtatcggc acgtcaagat
     3361 ggtgtagaag cctgtacacc tcatcgccca cgatcaccgg gccgccatcc cagttacgct
     3421 ggccttcggg gaagatgcct actgcgtccc ctgagtcgag gcggcgaagg atggaccgaa
     3481 tagatgcgat gtcccggctc cacctgcgct tgggcattgc gccgaacaca cggaagaacc
     3541 agcgagtaac aggatgcctg aacagctcat cggatgccac gaagtggata catcgtgtga
     3601 agtacatccc taggagtaga ggatcggcca ggttcacgtg gttacatacc accataaccg
     3661 gcccgtcttt cgggatgaac tcctcgccct ccgcccgcac attccacagc ccccggcaca
     3721 gagcgctccc cagcatcgtg agggtaagca tagtgttggg aagctcacgc aggtcgtagt
     3781 agagcgcctt ccataccggc aggaccatgg gcgtctgccg gcggtactct tcataggccg
     3841 ccccgtacct tcggcgcaag cgcggttgct caaccgcaag cgaccatgac acaaacacca
     3901 cacccagcat caatgcctca gcgagaagcg caaccgagcc ggtccagatc gccaggccta
     3961 tccagaacat ggtgtatccc cagtacatcg ggtggagaac aaacctgtag atacacttgc
     4021 tcaccaagta ccgtggttcg cttctcggcc accccttgcc ggcaaaccac aacaccacgg
     4081 cgccacgcca tgccacgtac gccccgagca ggcagacaca cgtgcccaca attgctctgg
     4141 gcatctcgag gggcgtatcc gggacatatg gagacagacg gggcgccagg tgcagcatgg
     4201 cccatggtag ggcagctagg aacaacgcgg cccaggccaa tagggccagc ggcgccatgg
     4261 cccttgccgc attgccggcg ccggagccaa tgccgcttga gttgggtgcc gaattgggct
     4321 tcaagttgaa catctcgttt gctgcctcca ggctgttggc tgactatctc caggcgatcc
     4381 ccaggctacg ttcatcgatc cccaggctac ttcccgaatg cgggtccggc aatcgcggca
     4441 accggaccgt ttggatcgac taacccctta catgccgacc acggcaccag caccacccag
     4501 gttccctgtg catagctgcc catctcgtag ggcccgaaga acaggtcgat gcctgcaggg
     4561 ctcacagtaa acctgttcat gttcttggcg ggtacctccc ggacctcgcc gctcacgacc
     4621 cagtcggcgc cgcgcgcctt aaggtcccgg atcacatagt cggaaactac tttttcccac
     4681 ccagtcccct ttttgaatat ctgatcgata gcaaaagcgg tcaccccgtt cttgccgact
     4741 ttcaggttca tagacgtatg aaaggtcatt ccatgagcac cgccggtgaa gtggtagacc
     4801 tcattcatca tgctgattag ggagctagaa tagtaacgcg tggtcacctc gctgaaatgc
     4861 tcccacgacc accatgaggc atcgggctcg aactcaagat catcactcat cggatccgca
     4921 ggatcactcc agatactagc agccgccgcc catacctctc tatccacgaa ggcttgcaca
     4981 tcctggagtc cacggttctt gccggcgccg gtgaacaccg ggtgagcgac atgtaccttg
     5041 atgaagccca tcctcatctc ttcggtggta acgatctcag ctgccgcagc cagttcgaac
     5101 gtgaatgact tggttccgtc ggcactggtc catttgccgc tccaggtgga cttcagaggg
     5161 ttgaaggctc ccgtccaggt cccggtcgtc ttgccatcgg cggtccgctc ggtcaagcgc
     5221 acgcgaccgt cggcgaacgt gcccgcaagc tccagcggtg tgcctatggt ctcataccag
     5281 taacgcccgg atacctttgc gccctcaatc tgcaactcca tctgaaccgc aaatttgcct
     5341 ccgatgctcc cggtaaaatg gtatccaacg ggcccgaagg ccagcgcggg ctgactttgc
     5401 gccagcgcca gagctaggca catacaaaac gacagcagga gtatggcctt acccgacggt
     5461 ctgctcagca tcaaaaccgc ctcccaacgt tcgcacaatt cccactcgca acctgggttt
     5521 acagcctgca agtcctgtgg gtcaagcata acacattcca cccggtcaca gccatcatct
     5581 gtctggctcc gggcgcgctg gcggcgcttc ggttgctgct ctccgagacc aagacctttt
     5641 ccatctggtt gggaggaagg ccttgcggaa aacccacagg actaggatgg accgtggcgt
     5701 ccatagtcca tggcatctgc tataggcaac cactgcggga atcaacgctt cgcgctccat
     5761 ggtaggcaac atatatgcgc catacgctgc gtgttcgccg ccaaccgtgt atctggctgg
     5821 ccttttgcga tgtgttcgtt cttggaacag gttttggcgg atgtgtcgcg aatccgaaca
     5881 acaggtggtg tatatcggaa cgtatatccg catagaagtg gagaatggga tgatttggtg
     5941 gctgcagcga cagatgatgg ggcgatgtgg cttgtatatg ggctattgtg ccagtatgtt
     6001 gcatctttgg tgagcccaaa tggggagata tgttatgccc gcttcgcctg ctgccaggca
     6061 tcgatccgct tcgtctacga ttgcaaccgc gtggccgggc gcaggatgtg ctatgattgt
     6121 gatgcactaa gactagcctg aggtgattac cgcatggact ccggtatgaa ctgcgagaag
     6181 gttgccgacg cactcggcgc ctggatcaag gaacgtgttc aggcagcagg ctgccgcggt
     6241 attgtgtttg ggctctcggg agggcttgac tcggctgtgg tggcagggct cggaatgcgc
     6301 gccatgcccg atgggtgcct gggcgtggtg atgccatgcc gctccgaccc ggaggacgta
     6361 aaggatgcgc tcgcatgtgc tcgcgccttt agcctgcctg cgctcgtgtg cgacctcacc
     6421 tccctcttcg accagcacct tgccctcgtc tctggctgcc tgtcggacgc cgccgcccgc
     6481 cccgatgtcg cgttcgcggc tcctgccctc acagccatgg ctgattctgc cgcacgcgtc
     6541 gcagtatcgg accccggccg agcccgcatg gcccaggcaa atctcaaacc tcgcctgagg
     6601 atgactgcgc tgtactacta cgccaacgtt ctgggctacc tagtagcagg cacaactaac
     6661 cgaagtgagc tcacggtcgg ttacttcacc aagtacggcg acgggggatc cgacatcatg
     6721 cctcttgcag gcctagtgaa atggcaggtg cgcgaactcg cctcatacct gggcgttccc
     6781 cggcatgtta tcgaaaaggc ccccacggcc ggcctctgga gcggccagac cgacgagggc
     6841 gaaatgggca tatcctatgc cgagctagat cattatatac ttaccggcga agctgatgac
     6901 gccgtacgca ggcgcgtcga ttctctaaac cgcgccgccc agcataaact gaccaggccg
     6961 ccagtgccga gtattcccct ggaatgagga caccagtcga tgccgtcagc cggtttcccg
     7021 agaagcacag tcctgccaag atccacacat tggccccctg atcaggcgaa gatacctgcc
     7081 aaaacgtacc taggaccgat acagcaatgt ctccctggtg aacatcttca cggccaccgg
     7141 caacactaca gcggctgcga tcacgctccc cacggccgac aaggccactg cgtcccactg
     7201 cagccctccg gcctgcgcca tccttcccag ggccgcgaat gggccataga ccggtatgag
     7261 gcatgcccac aaggccttcg cctgagagaa ggcagccaca aaccccggga tcatcacggc
     7321 aaatatcagc ggcgtggcgc ccgactgcgc gtcgcggaac gtgcgcgccc tcacactgat
     7381 catcatctga gcaacactca cgaccagggc cagcggtatg ccggtaagga gcgatcccgc
     7441 gagcacgaat cccggcaggc cttcactccc ggctgctccc tgcggcatga gattactcac
     7501 tgcccagaac gcggtgagcg taaggaccag cgggattaca gatatggtga acacagcgcc
     7561 cagtttgcct aggaagactg ccatgtcgga tgcaggggtc acgagcagcg actcgagcgt
     7621 tccccgttcc ttctcgccgg ctgttacgtc aattgccacg aacagaccac cctgtgccac
     7681 ggtaacggcg acaagtatcg tcaagtagaa agacgcaaag aaagccccgc gctgctccgg
     7741 cgtggccaga tcttctgtat tgagcgcaac agggaccagg atagacggat caacctcgcg
     7801 ggccgccatc ctctccgcaa ctaccatctg gttgtaggct ccaacagcca gttgtagacg
     7861 agtgtcggaa aatcctcccc caaagatgcc gccggcagtg ctgttggtga gcagacgcgc
     7921 catcgccggg cgttcctctg cgacatcctc agtgaaacca ggctcgaaca ccaggcctga
     7981 ttccttcttg gcatcctgga tgactgcttc gaggtcgccc tggtattcct caagggttat
     8041 cccgtacctg ccgaacatgt ccagaagcct tgcgtcggca tatgcgactc cctgcgccgg
     8101 gatggtgagc ggtttctccc tccgtgcggt catcgccttc atcagcatgg gcgtgaagct
     8161 ggcgtagaag atcccgatta ccagcggaat cacgatggcc tgcgtcatgc ccttccggtc
     8221 gcgcaccgtg tccatgatct ctttgcgcca tatgttcatc acgtctctta gcatcgtagc
     8281 tctcacctcc cgctcacctt acacgtcggc cggtgctgct caggccattg ccgctgccgc
     8341 tgttgccgcc gccgtgtgcg ttgccgtcag cattggcgta ctcgctcaca agaaggccct
     8401 gtgccccgcc tgctagagcc acaaaagcgt cctccaggct gtcgcgcccg gtctttccca
     8461 tgagctcggc cgggcttccc acggctgcga tgcgtccgcg gatgattatc gcgactctgt
     8521 cgcagagttt cgcagcctcg tccatgtagt gcgtggtaaa cagcacgcaa tggcccctgt
     8581 cgcgcagcat cctaatgaca tcgcgcgtct cgcgcacggc catcacatcc aagccgctgg
     8641 tgggctcgtc cattatcaca ttcttggggt catgcaccag cgcacggccg aggattacct
     8701 tctgccgttg cccacggctg aagccctgcg tgcgccggtc ggcaaactgg cccatgccca
     8761 gcatgtcgat gacggcctgc acgcgcgcgt cgagatccga gccggaaagg ccgtagaagc
     8821 caccgaaata cgtgaaatgc tcgcgcggag tgagtcgcgg atatgtcccg ctcgaatcgg
     8881 tctgcacgcc gagcgcccgt cgaacagcag ccgggttccg ccccacatcg ttaccgtcca
     8941 caattacagt gccctgcatc ggacggatca gcccgcagat catcctgaga gtcgtggtct
     9001 tgcccgcccc gttcgggcct agcagcccgg ttatgtgccc gtcagggcag tgaaaactca
     9061 ctccgtccac cgcccggacc tctccgtagt gcttcacgag atcccttacc tcgatcaact
     9121 agccccaccg cccttcccgg cccgcgcgcg gtcccaaccg cgcgcgccac tcccgccgtt
     9181 cgcgctactc ccgccgttcg cgcggttggc gccaccagca ttaccttttt gttttcgacc
     9241 cgcggcccgg gattcctcca ccgacttcat cagccgtgcg tcttaccggc gatgacacaa
     9301 ccgtaatgct ctcgatgacc acttcctcct gaatcgcttc cgggcgagac gggtcgcgag
     9361 tgcgcctcac ctccacgtga ggcagaacca ccgaaagctc gtgcgcgact gctgccgccg
     9421 gcatcaccct ctccacaatc atggtctgcc cggcgatggc gatctcgatg tagtctcggg
     9481 gcgcggcggc gccggccgag aacagagcag cgagccttct tagcacccct accgccgggg
     9541 ccatgtatgc cgcagggctc agtcgcgacc tccgaaccga agtcttccgc acaggctcct
     9601 gatcagcctc ctgcatacgt acgcgagcgg gcgcccgaat ccgacctgtc atatcagcct
     9661 cagtcctttc tcataggctt acccacttac caacatatga acaggcagga catgcggaca
     9721 cggcatggaa taactcgtgc atacgggcat agtcgactgg atcgtacgaa cggagttgta
     9781 gctgttggca aacagcaagg tcgctttcgc catgatactt gcactcctgt gtgccggggc
     9841 gctcttcttg gtgggcctat ccctttcatc gcatagcgtg ccgggctacc cggacataca
     9901 cccgctttcc gccggccgcg tcagcgttat tgccttttcg gtatgggcct tcacagcggg
     9961 gctattcgtc gtggcagcgg tggtgtttgc catagccgag cggcgggaga gcggaacccg
    10021 ctccgtgctc cgtctgccca ggactcaatc gcttctgacc tggctcgtgc tggccatggc
    10081 gctgcttaca gcgctaaccc tcatgaacaa tgggcaggca cgccgggaac tgccggccaa
    10141 ggagcccgcc gagtcttcgt cgcccatcgg cccagaggag ctctcgcaac cgcccccaat
    10201 gccgggcgca gagtcaagct cagaaggcaa gccttccgtg cggcgcgcgc ctacggcatc
    10261 ttcgccctgg gcagtgtatg cgatagccat ggccacgctg gccctggcag gagtcgttgc
    10321 agcgctcgcc ctgagccccg ggcgaaaacc gttcgctgcc gcccccgaag cggcgcctga
    10381 gcaagccggc gacgatcacg cgatcgaccc tgcgtggtcc gacgaagagg cgacctgcgc
    10441 catacagcaa gaacccgacc cgcgcgctgc ggtgatcatg tgctaccgcc tcttccaatc
    10501 catgctcgag caggcagggg tccgaatcga aaagcatcac acgcctgagg aatgcgggcg
    10561 aatcgcggtc agctcactca aactgccgcg tcgtgccgtg ttcaggctgg tcggcctgta
    10621 cagcagagcg cgcttcagcg agcactatgt cagcgagtca cataagtccg ctgccctggc
    10681 cgaggtacgc cagatcgcca cctatgtgcg cgagatcctg gcggcaaggg atgaggcagg
    10741 ggatgaggca gcggctgagg taagggatga ggcaagagct gaggctagag ccgaagcgga
    10801 gtcaaaggag gtgcccgaca ctgtcgcttt tgggcccaaa cgagcatgat ccgttcgcgg
    10861 actacattca catgttgctg gccatgtcgg tcatcatcat actgtctttg gacatatacc
    10921 ggctcaatcc tgtgtgggcg aagcccttca caatacgtct cgccggtttg agcttgatct
    10981 acgtcggcta cttcagggca tggcaggtga cccgcgcatc ggtagagggg cctgcgccct
    11041 ggaacacgcg ccgcaggcgc cacttctggg actgggtcga ctcgatgctc ccctggcgcc
    11101 gcaagctgga ggcgccccca cgggtatgcc ctgagtacga caccctgtac cggcagttgc
    11161 agatgtccat gcgtcgcggt cgctacttcg cacgctttct ggaagagcat ggcgccccgc
    11221 cccacgtggt ggagtatgcg caggcccatc tcgcggatag cagtaaccta cgccatgagc
    11281 aagtatcgtc agtattcgat atcatggagg agtcgctatg agtgtgtaca cgcccgaacc
    11341 cgctgatcaa tcggcgccgg ccattgcctg ggcctgcgac aagtgccgcg aggtcgtgtc
    11401 ggctgtagga acggtgatag tgggaaaaga cgagcagctg gagttgatct taacgactct
    11461 ggtctccaat gggcacctct tgctcgaaga tgtgcccgga gtggccaaga ccctggcggc
    11521 gcaggccacc gctcaagccc ttgggcttga ctttcgccgc atccagttcg tccccgacct
    11581 gctgcccggc gacatcatgg gcagcttcat ctacgactcc cgcgccgact cgttcaggtt
    11641 cagaagaggg ccgatcttca ctaacttgat tttggcagac gagatcaacc gcgggacgcc
    11701 caagactcag tccgccctgc tcgaagccat gcaggagcag caggtcactg tggaaggcca
    11761 gagttttgcg ctggacaagc ctttcatggt gatcgccact caaaacccgc tcgaattcga
    11821 agggacttac ccgcttcccg aagcgcagat cgaccgattc ctcgccaggc tgagcatggg
    11881 ttacccatcc cctgaggccg aatgcgagat gctggcgcga cggcagtcgc ggcaggcaga
    11941 tgctgtgata ctctcgcctg tccttagtaa acaggacttc ctggagatac agcgggccat
    12001 tgagacagtc cacgtgaacc ccgacataca caactacatc gtgcagatag tcacaaaaac
    12061 gcgcgtcgac cgccgcgtgc agctgggagc ctcgccccgg ggcagcctcg ccctgttcaa
    12121 gctgtcgagg tcgcgggcta tgatgcaggg gcgcgacttc gtgacgcccg acgatgtgaa
    12181 ggctgtagct gtgccggcgc tagcgcaccg ggtgcttctc cggcctgagc tctgggctga
    12241 gcgcatctcc ggagaaacgg tagtccgaga cgcgctggat gatgtgccgg tgcccaaggc
    12301 ggaatggagg tgagtctgct tgcgcacgcg tgtggccggt aaagcttatg tgtacatcgc
    12361 catgtcgggc gcgctcgtac tggccgcact tgcgactagt agaatcgagc tgttcatgct
    12421 ggccacgccc ctcatcttcg ttctgctctc gctggctttc gaagcgggtg ctgctgaacc
    12481 caagtacaca gtaagcaccc atctccaacg ggatcggtgt ttcgagggcg acagaacaga
    12541 ggtatgcgta accgtttcgg ccgcatcctg cgttccactg ctcagcctgc acatcatggt
    12601 ctgcgatgca gacaaccacg tgcttgcctc gcgccacttc acactgtcgc tcgggccggg
    12661 cgaaacacgt gtcttccagt ttgagctgga gatgcctgtt cgaggcgtgt tctacgtggg
    12721 tgatgtggtc tcgcgcgcct acactcgctc cgggctgaaa tcgtacccgg accgtgtgtg
    12781 gaccggaacg gagtgcatcg tgtatcccag gcctgatacg atcaggatag actggagcgc
    12841 gtcggcacgg tcacggcagc atgtgggcga atcctcatcc aggatgtccg gcgacgggct
    12901 cgaggtgtca gatgtccgcc tgcacatgcc gggcgatccc gttcgccgca tcaattggcg
    12961 cgctacccag aggcacggcc agctgtacat caacgacagc ctacgcgacc gcaacatcga
    13021 cgcggtggta gtagtggacg gcctcgtcga tctgggagag cccccacatt cctatttgga
    13081 ctgtgcctgc cgggccgccg ccagcatagc gctggcattc ctccggagcc gcgacagagt
    13141 cggggtgatc agatatgcag gcgcggtgga ctgggctgtc ccgcgcggcg gacggaatca
    13201 gctctacgcc atcttggatc gcctggcgcg cgtacgcact atccagagtt tcgtctcgcc
    13261 caacatgcga ctgattcccc gaagtgtgct tcccccggga tcgctagtgc tggtcatcac
    13321 gcccctgctc gacgacaggt ccatccagat gctcgcaggg ttggccgccc gcggttgtcg
    13381 ccccatggtg ctgtacgtgt ccccgatggg tctgcgtccg ctgacaacaa ctcaggcaaa
    13441 gcccacagat gatgccgccg ccctggccga gcgatggtgg aggctgaaac acaggggcaa
    13501 ggtcgcgcag ctcaggtcga tggggcttat ggtggccgaa tgggatgggg tcagcccact
    13561 gggagcctgc cttagcaacc atttcggcgc cccgggattt cgcccgggtg gagaggcggg
    13621 gggcagctcc gttaggggga atctgttatg aagatgacct gcacgaccct gcagcggtca
    13681 tggtggacga ttgcggcgcc cactgcggtg tgcatcatga cgacgggcta cgctgcgctg
    13741 acggcgccca catggtgggt agttgcggtt gcgtttgcat cagccgctct atgcgccgca
    13801 ggtgccgcag cgggcgattt cggaatcgtc ggatggagcg gagctgcggc caccggtgca
    13861 tgtgccgcca gcctagtcga aaaccgcccc atccattggg gagaacccac tctgatgtgc
    13921 atcggcgtcc tggccatgct cgaaatcggg cacatcgtat gcatcatccg aactcgcgaa
    13981 gtgaatccgt cagtgttcgt tcacgctcat gcagatgctg ataaggatac gccgaaagat
    14041 gccgataatg gaaatgtgga tcacaatgaa acactcatag ccctgctgcc atcggtggca
    14101 ctccgactgg cggcgtcggg cgccgcgtcg ctcgggctga cacgtctgta tctagcctct
    14161 gctatctcgg gcgcgctgcg cgattccgat acgttattca tgatgttgct gggtgccgtt
    14221 gcagtgggag cgtgtatgta ctggctcgtg cgtatctcaa tacaggattt tcgcagatag
    14281 tggcgaatat gttatatcgt ccgccaattc gtgacaagct gctcagagca gtcgatcgga
    14341 ggtctgccgt gtgagcgttt gggggcctgt ggacggttct tgctctggca attctgccgc
    14401 ttgctccgac tccttgacaa gcggaaccag cagcttcata tccacactgg ccggcatgac
    14461 agagctcgaa gtgttcttcc tgagcccgga tttcgttatc ctgttcgcga acgacgcggc
    14521 cgcaaaagca gcaggagttt caccaatcga cctgataggc cacaggattg acggcgtact
    14581 gcccatgttg taccggcggg ttgagatgtt ccgtcctgag ctggaacgca cgggcgcagt
    14641 gttcgtcgat cagagtgcac cagaaatgca acaaggttgg ctcgttctgc gagatacgta
    14701 cgtggttctc atgcgcaaca ttcttgagga ttccccattg ggttgggtag tagccagcct
    14761 ggatccgtcg ctgcgcaccg gcctacataa tgctgtggaa atgattcccg tgcccatggc
    14821 tatcctgcaa atgcccgaac tcagcattga ggtggccaac tcacgcttca caacgctcct
    14881 tgcgccatcg gagtcgctcg ctgcgaccga gcccggtcac cccgggcaag cgatagccgt
    14941 gatggcaggc gcggcggtgc gcaccggaga gtacatgaag tccagcatct catggccggg
    15001 tagtcccgac aaggggcgca ggactgactg gacaatctac tgctgcccgc ttcagcgttt
    15061 gggccaaaac agccgcgata gcaaggctgt ggtcgtcctg gtgcagcaga cacaggcacg
    15121 cgcatcgatg cttcggaatc ttgacgtcgt gcgcaggttg ggttcgtctc tgatcggcag
    15181 tatgagcatc ccatcctttc tgcacaacgc tgtgtcagca tgcgcatcga tcctatccgc
    15241 gccgtactgc gcgatcgtcc aacacgatcc gcttggtaat cggctgctga gactggccga
    15301 atcaagcccg ctaggtctga ttcgagagtc cgctcccgtc gagctctacg gctggctggc
    15361 cgacataatg caatcgaaag agtcttcctg ccgcgtaatc cagctcgaca agtctccagg
    15421 gtggcccgag cgcgagcagg ccgggatcga agcattcgcc gtctttccca ttgcagctca
    15481 tggcaggtgc tacggacaca tagtagcggc actgaccgac gacggcagca atcttaactc
    15541 cgacgacatg cacctggcgg aactgacggc actctactgc gatattgcgt tggagcaaga
    15601 gcgagcggcc agcgactacg cacagctcga agccgcacag caaaaggccg aaatcgaagc
    15661 ggcacagact gccaagcttc tcggtgcgct catcgacggc cttgaagacg gcgtgatcgt
    15721 cgtcgacgca ggtcaccgcg tgatcctggc caacgaaagc gctgcggcgc ttcttggaat
    15781 gtcgcgggat gagatgcaca acctcgatca agtcctcgcc cgcacccaac ttgcacagat
    15841 ggacggaacg ccggttcctg atcaagaccg gcccgtacga aggctgcttc agggccttca
    15901 tgtccttagg ggagactatg tgttgacagc gttgggtgga tcctcccgcg tgctcacttt
    15961 caacggcgga gtactgagaa atccagacgg cagcatatcg tttgccatta cggtagcgca
    16021 cgaccgcact gagcttgcac aggccgagaa agccagccgg gactacctga gattcgtctc
    16081 gcacgatctg cggtcccccc tcgcgctgat ctcggccagg gctcagatgc tcgaaagatc
    16141 gcccggtgat cctgacagcg tacgtaagaa cgcccaggcc attctgagat ccgtccgaca
    16201 gatgaacacc atgatatccg acctaaccga ctctgtccgc ctggagttcg gcagcggcct
    16261 ggccgtacgg ccccaggcac ttgacctagt cgctctactc aacgagttga tcgaaaggtg
    16321 gcgggagaca cccgagggcg accgcatcga ggcttgtttg cccgccggtt caatgccgcc
    16381 tgtgtgcgcc gaccctgacg ccattgaacg cattcttgca aatctcatat ccaactcgct
    16441 caaatactcg ccagatgagg agacgatagc agtctcggcg acagctaacg acctggaggc
    16501 gaccatatcc gttgcagacc gtgggcccgg aattcgccct gaagacgccg ggcgactgtt
    16561 cgaacgcttc ttccgttcgg aaaacgctcg gaagcaccac gatggcctgg gcctgggtct
    16621 tcccatatcc aaggccctcg tggaagcaca gggcggccgc atctggatag agaccgagct
    16681 cggcagaggt agcgtcttct cattcaccct ccccctatca ctactgtaac catcaagttc
    16741 ccggagcggc tgacgtatag gcctcaatgc cagccaactc ccacgtaggt ggtagtcccg
    16801 cccatcagtg aaccagattg tgcgagaagg aacaatcgca ctgatgtcgt attccttgtt
    16861 gttgccggag ttgagagggg gtgaaacgat tgaggattcc caagtcaaag ctggtattgc
    16921 ttgcagttgt gattctggtt gcggtatcgg ccgcaatggc cgggctggcc ccggcagtgg
    16981 cagaggctga agagagctgt gttgattgcc acaccaacaa agacctcctc atatccctga
    17041 tccccgagag agaagaagag gccgcagaga ccggagagtc atgagcgggc cctcctcccc
    17101 cggcggggga gccgtatgag aaagtcttcg tggatccgtc tttccttagc acaacccatg
    17161 gctacatcgg atgcacatcc tgccatgggg gcaacccgga tggaaagacg atggcagaag
    17221 cacacgttgg agtcaatgcg tcgccgtcta gccttgccga gcagaactgc actccgtgcc
    17281 acgatgatgt ggtggcaact tacggcacaa gcctacacgc tacattgcgc ggcatggaaa
    17341 acgggctata ctccttcacc ggagaggcca accggggcaa ggccaaggaa gcgtttgaca
    17401 agtactgcta caagtgccat gccacgtgcg gcacttgcca cgtgtccaag cccgaggcag
    17461 ccaacggcgg tctgatacag gggcacaagt tcttcagctc gcccgaccag gactccacat
    17521 gtaatgcgtg ccacggcggc cgcgtcgcca acgagttcaa aggaggaatc gaaggattcc
    17581 caggcgacgt gcactacgag aagctcgggt ttgattgcct gacatgccat gacggcaagt
    17641 ccttccatgg cgacggctcg tcgccggcca acaggttcga ggcacccggc aaaccctcct
    17701 gcctggattg ccacccggat gcagcacccg gcaaaggcac tgtggatgcc cacaatgcgc
    17761 atccagaaga cacgatggac tgctacgtat gccacgcgac ggtcaacaag agctgctaca
    17821 actgccatgt gggcgagggc gcccagtcca agttggactt caaaatcggg ctgaacacgc
    17881 gcgccgactg gccgtacaag tactccgtag tcaggcacat gccgggagtc tccaccatgc
    17941 tggatccgtt cgagcccggc ctgatggacg actacgactc tgtgccgaac tggaagacga
    18001 ccatggtaca caacatccag cggattactc cgaggaacaa gacgtgccaa tcgtgccata
    18061 acaacagcaa ggtgttcctt accgagagcc ggctagtacc cggcgattct agggccagcg
    18121 agaatgtggt tgtggagcag ttgccgccca agttctagct ggtaagtcct agtgacgaac
    18181 tgatcaatgg acctattgaa ctaaggagga gtacgtaatg aagaacacta ttcgagcagc
    18241 tttcgcagct atgctgatcc tgaccctatt tgcagtcccc accttcgcgg ctgaagacgc
    18301 agtcgtcaaa gcggtcgaca atttcgtgag caatctgccg tccgactggt tccagatgaa
    18361 gccggaagtc ctcctgcaaa agctcgacct gggcgaggag atcttcgtgc ttgacgtacg
    18421 ccgacccgac gaattcgccg ccggccacgt cgaaggcgcc gtcaacattc ccgtgcatga
    18481 tctggccaag catgtcgctg aactgccgga cgaccccgat acccttatcg tagtctactg
    18541 ccgcagcgga gtccgctcca tgttcgccac ctcagccctg ctcgtcctgg gctacaacaa
    18601 ggtgtacaac atgcccggcg gcttcctcgc ctggcaggac gcgaagtatc ctgtggagaa
    18661 gtaaggccgc tctggcaagc tggacgacca aactgaccct ggcaacaaga ccccgtggcg
    18721 ccatgcgccg cggggtcttt gtagtccttg taggcatcgc gaagcggaag tgcgagcaag
    18781 cattcccggg cagagtgatg gtagaaaggg tcatcggcaa aactgtcagc aaaaactgac
    18841 ggcgtgcctg cttggatacc cctcaagaat tctgatgagc tttactatgt cccggctgcc
    18901 ctgggcagtt cttccatgcc ttaccgtttt cggtcgaccg ccacggaaat cgcctgccca
    18961 acccattgaa tagcctgcac gaacacgacg agcactacca cagtggctat catcacatcc
    19021 gtgcggaacc gctgataacc gtatcgtatg gccagatcgc ccagcccccc gccgcccacc
    19081 gccccggcca tcgccgagaa gctgacaagg ctcacaagcg ttaacgtgaa cccgagcacc
    19141 agcgacggca acgcctcagg aaacatcacc ttgaaaagca cttccagcgg agacgccccc
    19201 atcacaagcg cagcctccac aacgcctcgg tccacaccgc gcatcgagct ctccaccacg
    19261 cgggcggcga acggaatggc gccaatagac agcggcacaa tggccgcctg agtaccgata
    19321 ctagttccca ccaagagtcg cgtcaaaggg ataatcgcca ccataagcac tagaaacggc
    19381 agtgacctac ccacattgac gatagccgac agaacctggt caatgcggaa gatccggttg
    19441 atccgccagt cgcgccccaa ccccgggtcg gagaagaaca caagcgctgc tccgaccaga
    19501 gttcccaaca cgatggaaat ggcgcccgac acaaccacca tccataccgt ctccaaaagt
    19561 gcagtcctga gcaatcgata cagcatcgca atcaactcca cgaaagcgta gtaaccatcg
    19621 ccttgagaat acgcgacgtc ggggtgcgca gcacttcctg cgcagggccc ttctcgacta
    19681 tctgtccttc gtcgattacg acaagcgaac cgcaggcttg ctgcgtgata ctgagttggt
    19741 gtgtgacgat gagaaccgtc accccgaaat ccgcattcac ctgccggatc agctccagta
    19801 tggactgagt ggttatcgga tccagcgccg aggtcggctc atcgcacaac aagacggcag
    19861 gattggtcgc cagcgcccgc gcaatcccca cccgctgctg ctgacctccg gatagcttag
    19921 ccgggtatgc gtcgcgcttg tcggccaagc ccaccacatc gaggagttcc tccgcccttc
    19981 gcttcgcttc ccgggcaggc gtccgcgcaa tctccagcgg gaacatgaca ttgcccaatg
    20041 ccgtacggtg cgaaagcagg ttgaaatgct ggaaaaccac gcccagccgg cgctgcgcct
    20101 gcctaagttc cgcgcccgca agcttcgtaa tatccacatc gccaatgtgg atcgagccgc
    20161 tatcgacacg ctcaagacct gtcatgcacc taatcaacgt gctcttgccc gcgcccgata
    20221 gccccacgat accgcatatg tcgccctgct caaccgcgaa cgaaaccccg ctcagcgcag
    20281 gcatggactg cccgggatac ttcttcgaca cattctcaag gctgatcatc cggcaaacac
    20341 ctccccaaaa accccaaatg ggcctcatgc atgccctggg tagcaaaaag ccctcccgct
    20401 cctggctaaa tgccagggac gagaggaccg aaatccttcg tggtaccacc ctggttcgcg
    20461 cacccctcgc ggagaagcgc ctcgacaagc gcggcggacg agaacttgat tgctgtgcca
    20521 cacctcactg ggcacgggca atgcctgagt tccacgaccg cgatgctccg gcgctataac
    20581 gggcgcgccc gtccgcgcct actgatccct ccctgccgac cccgcttttg ccggcaacac
    20641 ggtttgggct ggactttcgg gcgaccgctc aggggccatg ttcctcgcgt ttccaaagcg
    20701 ccggctctca gcctgggcca gctctctgta gcccgtactc agcgagtact catccccgtc
    20761 aatgcgtatg tcaatatggc tgctaaagac cattttgtgc tcttgacagc aagattagca
    20821 cacaggccct gcattgtcaa cactcgcacc cgcaccgccc agcagttctg caaatgcgtt
    20881 gcgaggcagc ggcctgtgag ccgaccggga tccagggacg tat
//
LOCUS       DAOTAC010000004        20870 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_2085_length_20870_cov_5.017247, whole genome shotgun sequence.
ACCESSION   DAOTAC010000004 DAOTAC010000000
VERSION     DAOTAC010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 20870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20870
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2085_length_20870_cov_5.017247"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..868
                     /locus_tag="PLM74_00370"
     CDS             110..868
                     /locus_tag="PLM74_00370"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HON41570.1"
                     /translation="MHSTHSPDARASLDELCESAIQCDLAGVCFTEHVDFDPSDAAYG
                     YINYEEYLSDVDRCRDKYGDRLEIKAGIEVTYQSEYVDDIRRFLEAHQFDYVLGSVHL
                     IDHTFIGSPAYFVGKSEAEACGPYWRETMAMLESGLFTRIGHLDYIRTRWPREYGLPG
                     LARWRRSMTDILQLVIESGAIIEVNTSAIRRQLGGPYPNWETLELYRRLGGTRVFMGS
                     DAHNAPNVGQYFPEVAARLEQTGLVICSGCEVLC"
     gene            1027..1641
                     /locus_tag="PLM74_00375"
     CDS             1027..1641
                     /locus_tag="PLM74_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HON41571.1"
                     /translation="MAGDNREEIGSRQAPPCPGGFLHQIRPGDTLYALASRFGTSVDA
                     ILRANPGTDPASLRVGQLLCIPAAAAPGRPPGVPPGRPPGAACPGGFLYQIRAGDTFY
                     SIARAHGTSVDALRRANPRVNPNALRVGQLICIPTALAPGVPSAPACPAGATPYIIRR
                     GDTFYGIARRFRVSVAALTSANPGVNPGRLQIGQLVCIPPRTGG"
     gene            1817..2269
                     /locus_tag="PLM74_00380"
     CDS             1817..2269
                     /locus_tag="PLM74_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41572.1"
                     /translation="MAIDSQLIEVSSCLVDDYSPLVESGQWRVAAICTSEQNSADAIM
                     QLERHNETDEVFVLMRGKAILFIATGQGEWGTGKINNVEMESLQVYNVKRGVWHACAL
                     GPDAVVLVVENSGTGRSNSDYSQLTTDQRRLVIDTCAGLTGLTGLTQD"
     gene            2287..3594
                     /locus_tag="PLM74_00385"
     CDS             2287..3594
                     /locus_tag="PLM74_00385"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HON41573.1"
                     /translation="MALAQTEYMAKREIVPGRELQRQLRQRSMVFALLSAAYFMSFVQ
                     RVALSVVAAELAEELHIGTVELGLMSSAFFVAYAAAQPAVGILSDLFGPMIVVSCCLC
                     LGTIGTWMFAASRSMTTAFVGRILIGAGLSGIFIPGVKMITMLYDADRFASVNGLFLA
                     LGNAGAVLGTAPMAWLASAAGWRGSFDIIGVLLIVLTVMCWFVSRAQKSRVTDCTPRG
                     AGGTLAAVKVFARDRNLWLVSLFMFARYGSQSAFQSLWGIPHIMSAHHVSREQAAAAV
                     MMIGVGYIVNAPLVGWVADRLSRRSGNAFQARRSILAWTTGAYALTWIPIVFFPGRLS
                     IGALYALLFTMGLGASSGGLGFAIVKDQYPPEAAGSATSAVNIMSITGAAAIPPIVGV
                     LINGASVAGAGIRAVYGLSLWPCLAAGVCAWLAAMLLADAPVS"
     gene            3829..5022
                     /locus_tag="PLM74_00390"
     CDS             3829..5022
                     /locus_tag="PLM74_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HON41574.1"
                     /translation="MTYQLHLEKWGRVLVSIKKKRVLILAACVALCLGLFAYGSAAEK
                     VVKLGWVGSLTGDQAIWGQCELNTVKMLAEELNAAGGLLGAKVEVVGYDHRGDAMECV
                     NAVRRLTSQDKVVCILGPNASGHAIPIASVLEEMKVPDIATVATNPKVTVVDGKVKPF
                     NFRVCFIDPYQGAVAAGFAYDVLKLTKAAVLYDVADDYSQGLTEFFIKTFTEKGGKIV
                     AKEAFKFGDTDFRPQLSKIKAANPEIIFMPYFFKEVALSANQARELGIKATLMGGDGW
                     PSEVLIQMAKDAVHGSYVVNHLDYDDPDVQDFKTRYTAKYKRAVELNGYLVHDAFLMF
                     VDAVKRANSFSPAAIRDALESTNIMGITGRIKINPATHNPEGKEAAIIRIMDGNYVFQ
                     QKYAP"
     gene            5165..6043
                     /locus_tag="PLM74_00395"
     CDS             5165..6043
                     /locus_tag="PLM74_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HON41575.1"
                     /translation="MAQFLQQMINGLSIGSVYALMAVGYSLVYSIMSFSNFAHGGVIM
                     LGSYFGFYLLTLAKWAFPAAFVGAALCSAALAVIVERVAYRPLRLRNAPFLYFIISAM
                     GASMFLENFVVATIGPTFKTYPTVLPRAPIEIGPIYIGRLDIMIFIISAVCLALLVYF
                     LDRTKVGKAVQATAHNARAAKLMGINTDFVITLVFAIGGFLAGVAGVMFGMKYTVYPQ
                     MGGITIKSFIAAVFGGLGSLPGAVIGSLLLGIIETLISGYFSSQFRDLISFTLLIAVL
                     VFRPMGLAGKATEEKA"
     gene            6055..6975
                     /locus_tag="PLM74_00400"
     CDS             6055..6975
                     /locus_tag="PLM74_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HON41576.1"
                     /translation="MNWFYIKGILMLAGMNLMAVLGLSLLTGFTGLFSFGHAGFMALG
                     AYAAAWAGVRMGLPFLVALLAGGLFAGLVSLPIGRLTLNLKGDYFCIATLGFGEAIRL
                     VLDNVQTFGGARGWPGIPIRTDLWNVALICVAATLFMVRLVNSRHGRNMIAVREEELA
                     AKTVGIDTFKYKMLSLFVSAVYAGIAGGMMGYYTGFLQPKMFGMAKSTELTIIVIFGG
                     IGSVSGSVLGSILLTALPEILRTFAIWRLVVYGAAVIFIMISRPEGLMGGKEITLDAI
                     RRFLCGRKAQTGAGAGTGAAAGAEGGEVGP"
     gene            6972..7748
                     /locus_tag="PLM74_00405"
     CDS             6972..7748
                     /locus_tag="PLM74_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007936067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON41577.1"
                     /translation="MNILETHDLTKTFGGLTAVEDVSFGVRSGSISGLIGPNGAGKTT
                     VFNLITGIYKVTAGQVVFDGKPIHNLEPHRIAQAGITRTFQNIRLFKRLSVYNNILTA
                     CHDLAGYSVADSAFRTRRYGEQEKQLKREASRLMDVMGLDEWRDKQAVSLPYGVQRRL
                     EIARALAVRPRLLLLDEPAAGMNPDETRQLMTLISRIRDEFGCTILVIEHRMDLIMGI
                     CENIVVLNFGRKLTEGPPRAIQSDPQVIQAYLGEGVKTGA"
     gene            7741..8448
                     /locus_tag="PLM74_00410"
     CDS             7741..8448
                     /locus_tag="PLM74_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON41578.1"
                     /translation="MLEVRNLHVHYGGIHALRGVSVSVGQGEIVTIIGANGAGKSTLL
                     NAISGIVRASEGAMTFKGSPLPAASYAIVKHGICQVPEGRLVFANLTVLDNLMLGAYL
                     RHDHQAIRKDLDRVYELFPRLRERASQMAGTLSGGEQQMLTIGRGLMSDPELMLLDEP
                     SLGLAPILVETIFQVIEDIRRAGKTLLLVEQNAHKALSAADRAYVLEHGQIVKSGNAR
                     ELLSDPSIQESYLGVKQ"
     gene            8563..9465
                     /locus_tag="PLM74_00415"
     CDS             8563..9465
                     /locus_tag="PLM74_00415"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HON41579.1"
                     /translation="MRREDRDGRRSNADLGNGRGLGGGGRGAEPACAQSLPQYEGFFA
                     EFYDILHSSYDADLKMYLDLARAHGDPVLELGCGTGRLLIPLAAAGHTVTGIDLSRDM
                     LTRCQGKLDIEGRSVAGRASLIQGDIRRFELGRRFNLIMAACNTILHCTASQDLLAVF
                     ARVREHLAPGGVFVVDFSIPNVKAMIESSGEEEVFEVIHPVRGSRIVDTYKATFDFAR
                     QIETIDTRLEEWDGGSLVRWARAQSERGIYFPREVALALTCSGFRIAKTWKGYRRGPL
                     VETTQDIVYICRVDASPSPSERPQ"
     gene            10768..11064
                     /locus_tag="PLM74_00420"
     CDS             10768..11064
                     /locus_tag="PLM74_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41580.1"
                     /translation="MEHGSALCLAPVMLDGVDYDNHSWAARMQAWLASLPYDAQLRAG
                     SFCELMREAFDADRAGMYLKSLSILGRAERLLADCDSLDSFIYAMGGHSTPDSN"
     gene            11277..11984
                     /gene="grpE"
                     /locus_tag="PLM74_00425"
     CDS             11277..11984
                     /gene="grpE"
                     /locus_tag="PLM74_00425"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HON41581.1"
                     /translation="MELPDSVPVTQDFSGPIPGPDVGPDPDLASELGREHMPEPQLKP
                     EPEPGPGRPTSHAGIAAEIAAAQGKLDQLIAMFADKIQYDDQKEKAFSRLYDELDSFK
                     RDFVDRRMKEAYTDLILLYDNIKNYTGDDDVMKEEGALARRYLGYVMEELTEVLARRG
                     IEPIFAGPGVSPNAGSFDPQFQRAIRTVPAESRDEDGRVVRVVKDGFTKDGKVLRPQS
                     VIVARWTEPPGPAGGGV"
     gene            11984..13516
                     /locus_tag="PLM74_00430"
     CDS             11984..13516
                     /locus_tag="PLM74_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002201486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HON41582.1"
                     /translation="MSRAIGIDLGTTFSAIAVLNEFGKAEIVPNAEGERVTPSVVYFG
                     DDDCVVVGSVAKNSAVSDPHNVVEFIKRQMGNADYAFTHGDTEYGPEQISALILRKLK
                     QDAERLLGEAVNDAVITVPAYFDDTRRRATINAGRIAGLNVLRIINEPTAAALAYGLT
                     NASDQRIMVYDLGGGTFDVTIMDVGRGEITVVATDGDHMLGGNDFDDQIMLYVNDAFK
                     REHGVDLLAEVEVQQDLRQRAEAAKKTLSSRPSAKISMSASGKRLTVELTREQFEQMT
                     GHLLERTKMLVESVMSEAKTGWGQIDTVLLVGGSTRMPMVRQMIERMSGKAPSQAINP
                     DEAVAMGAAVQAGILMAQKGDASILATAEGRRLANTRVTDVTSHAFGIVLMDDRTKTE
                     YNQVMIARNTPIPHEVQDHFYTISNNQARVKFVVLQGQDRDPANCNTIGETELEFDRP
                     KPRGYPVRVTYQYDANMVMHCYMEDERTGRIAQLHISQSGRMSDREVEAKRRLMDRVK
                     VD"
     gene            13556..14272
                     /locus_tag="PLM74_00435"
     CDS             13556..14272
                     /locus_tag="PLM74_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HON41583.1"
                     /translation="MAKGAIHRVWADYDATYGGRRGIMIHASMAVTERRSAQSEQVLL
                     IYWFYYTDGKPVPGASGEFTDSGGNACVYEGVDTPYPSTEYDDFSVFVPYEALSHQGS
                     GSFSAYANAALFEGNSVIAWAPRVLSFTLTIRDVHGPQPHGQPRPKSTPKTTTATTPN
                     TGGSGGGGRSFDSQTPAQRYRQMFGIPPGASPAQIKQILDREYQKYRARVNSPDLSAR
                     QEADRMIMEIGKARRVLLPQ"
     gene            14310..15842
                     /locus_tag="PLM74_00440"
     CDS             14310..15842
                     /locus_tag="PLM74_00440"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HON41584.1"
                     /translation="MKAVGIDLGTTFSALAVLNDYGNPEIVPNREGERITPSVVLFDA
                     ENQVTVGAIAKNTAVSDSENVVEFVKRQMGNPDFVFTHSGREFRPEDISAMIIKKLKT
                     DAETLLGAELPSAVITVPAYFDDARRQATMNAGKIAGFNVLGIVNEPTAAALAFGMSN
                     PGIHQRILVYDLGGGTFDVTLMEVAEGKVRVVATDGDHQLGGKDFDDKVIMFCNDEFK
                     REHGIDLLDDLEALQDLRQRAESAKKTLSSRDSAKITISAQGARLTVEITRKQFEEMT
                     ADLLVRSEMLLDAVMSASGLEWADVDQVLLVGGMTRMPAANEMIRRVTGKEPTLSVNP
                     DEAVALGAAIYAGIILAGRGDPEVVLSEAGRRLRDTRIEDVTSHSYGVLLVDSKTGDD
                     INEIMIAKNSPIPASHTATYYVHEDGQSSVELVVLQGEDRNPANCNEIGRVVLPLGGS
                     KPRNYPIDVTYEYDANMIMHATIKDPQTGASKSLQITQQGRLTNAEVEEKRETMQEVE
                     VV"
     gene            15842..16981
                     /locus_tag="PLM74_00445"
     CDS             15842..16981
                     /locus_tag="PLM74_00445"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HON41585.1"
                     /translation="MEADAERGARTRALYEQLLAPVARQVDARLADEAHNLPAELRDM
                     YDCAVGGGMRFRPCLMYLAYLVCSAGVDGEGGRGGVEGCGSRVGIDSLIQLASAVELL
                     HKASLVHDDLIDGDALRRGQQSFHATFGSERAVVMGDLLVAMAHDVAERMRPVLPAGV
                     YCQIRFHFNKAHLDLCRGELLELVAARRERPLARDYVDRVIEGKTASLMEQSMAMGAI
                     MAGAPAACVDALARYGRYMGFVFQTINDINNLTGFDLDIKGQAMTDLALGRVGYHVLG
                     LEMTAGMEQVGLLASEIKRVERADDGLRLRKLVQKGRLRIWLDGVICEYADQARQALK
                     ALPECGARDALDSIGREMFESWFWNPESSIDCLGGGCSEKCSPSD"
     gene            16956..17561
                     /locus_tag="PLM74_00450"
     CDS             16956..17561
                     /locus_tag="PLM74_00450"
                     /inference="COORDINATES: protein motif:HMM:NF021506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HON41586.1"
                     /translation="MRSARPLTESALAAAVCVVVWALSMGLPGLGGYVRYMTPSLIAI
                     VGARHGARWSLAACAVTGAGIGITLGPTQLLISLLLGAPTAVMLPLMKQRVAPALWWG
                     LASAVYAVAVTGLGCGLAALYGAGPRDYVSATASANYAALGGLWAWLGVGPGELAAFA
                     GRSAWFILPGVGVVSGVMMYLINVVPYRIGLRTLDRTRRRR"
     gene            complement(17527..18048)
                     /locus_tag="PLM74_00455"
     CDS             complement(17527..18048)
                     /locus_tag="PLM74_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41587.1"
                     /translation="MLMESLVLAVDDREAKQVVSGIASSAEFVVRGVRFEQGVIVVDT
                     TVRAPVPIHPSFRVEVVAARGASLTVRISGPMGLGAGPLDVVINRIAPRLPQGVSYLG
                     RGVITIDLVQASRGALKSLDMAGISIQPGQAVVRVSSADVDLAALAQAKGSASTRSAH
                     AGATGDGASGRGF"
     gene            complement(18056..18952)
                     /locus_tag="PLM74_00460"
     CDS             complement(18056..18952)
                     /locus_tag="PLM74_00460"
                     /inference="COORDINATES: protein motif:HMM:NF039826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5685 family protein"
                     /protein_id="HON41588.1"
                     /translation="MFGYARPQKLDLTLRQGHHYTAHYCGLCFALARDYGTLWRALTN
                     FESTLLVLLMSAQADADPSFRKTICPVSAFRRFAIVSEHEGFMTLGAAFTVCMAELKS
                     LDAVRDGSLLARPARDIGGRQFSRARRVLAEHSFDIAPLLQADDRLAELEAEASAAPG
                     SITFDELTAPTADGLAHVFEYTGALAGRDESCAGLARLGKAVGRLVYLGDCLEDVARD
                     AKRGAFNGLSACMMVDQTSAELSPGAADALSYMIARCNVEARHALSQLTLNRYRPIIE
                     NIILLGLDIRVRRLLQARTKKE"
     gene            19156..20238
                     /locus_tag="PLM74_00465"
     CDS             19156..20238
                     /locus_tag="PLM74_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006285417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HON41589.1"
                     /translation="MRRIIMVLGVIVIVLLAGFAGFLGFISITDYRPKDVIRVAVDNP
                     QGEVIPVGREITITSFNTGYCGLDKHADFFMDGGTMSRAVSREQTSANIDRISSFLMG
                     QDSDIYLLQEVDRKSRRSYGTDQYRRFCADLAGYNSSFATNYKVAWVPVPLADPHGNV
                     YSGIATLSRYRVDGAARYSLPGEFTWPMRLFELDRCMMESRLPTGDGRQLVIANVHLS
                     AFDKGGFIRTQQLAFLKEYAARELESGNYVVIGGDWNHLLSSDPEAKKAEYGESWPFW
                     LQVLPDDFVIDGFRWAVDETTPSCRTLDKPYVPGENIEVNIDGFLVSPGVEVVHVHGH
                     DLGFENSDHNPVTVCVRFGEEVPGQD"
     gene            complement(20204..>20870)
                     /locus_tag="PLM74_00470"
     CDS             complement(20204..>20870)
                     /locus_tag="PLM74_00470"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HON41590.1"
                     /translation="EEALLPFPGYYMFDSIDESSGQSLRGPAAAQASRRVCWPFKLRS
                     DDTRLALRIAFAALVIAVIALTAQIWAPRNTTQRLASEPQPELRPQLQAELQVEIEPS
                     IQPQAEPQAQPQAAPEPEPQQAQAESQAQQAQVATHGNAPRVAVRPGEPGTVIVTVAP
                     GESVAVIAKRLYGRATDERMREILAMNGITDPRKISPGTTLVCPKPPGWTASPDPALP
                     PQT"
BASE COUNT         4249 a         5832 c         6527 g         4262 t
ORIGIN      
        1 ggggcgcttc agaagtggcg ccttcggaca cgccgcacac gcttgtgcta tactgagcct
       61 gtgttgccga aagagaggtg caagacccac gagactgttt gaccatcata tgcactccac
      121 tcactcgcct gatgctcgcg cgtccctcga tgaactgtgc gagagcgcca tacaatgcga
      181 cctcgcgggg gtgtgcttta ccgagcatgt cgattttgac ccgtccgatg ccgcttatgg
      241 ctacatcaac tacgaagagt acttgtctga tgtcgacaga tgccgtgaca agtacggcga
      301 ccgtctggag atcaaggcag gcattgaggt tacttatcag agcgagtatg tagatgatat
      361 ccggcgcttc ctcgaagccc accagttcga ttacgtcctg ggctcggttc acctgatcga
      421 tcacactttc atcggaagtc cagcttactt cgtgggcaag tccgaagcgg aggcctgcgg
      481 gccgtactgg cgcgaaacca tggccatgct cgaaagcggg ctcttcacga gaatcggcca
      541 cctggactac attaggaccc gatggccgcg tgaatatggg cttcccgggc tggctagatg
      601 gagacggtca atgaccgaca ttttgcagct ggtgatcgaa tcgggcgcca tcattgaggt
      661 caacacctcg gctatccgca ggcagctggg cggaccatat cccaactggg agacgctgga
      721 gctgtacaga cgactcgggg gtacccgggt gttcatgggg tcagatgccc acaatgcgcc
      781 aaatgttggg cagtacttcc cggaagtagc ggcccgactg gagcagactg gcttggtcat
      841 atgcagcgga tgtgaggtgc tatgctagga acctccgcag ttggactgcc gtgggcttgc
      901 tgttaacacg ccgttggcct gacattgccg tcatggtctc agggtcatat gtactcccgg
      961 ctcggggaca ggtgagcgcg ggtatgcata tggtgcattg gactcaaccc taaggagtga
     1021 acggtaatgg caggcgacaa cagggaagag atcggttcgc gtcaggcgcc gccctgtccg
     1081 ggcggattcc tgcatcagat caggcctggc gacacgcttt atgcgcttgc gagcaggttt
     1141 gggacatctg tcgacgccat cttgagagct aacccgggaa ctgacccggc ctcgctccgt
     1201 gtcgggcagc ttctctgcat cccggctgct gcggctcccg gtcgtccgcc gggtgtacct
     1261 cccggtcgtc cgccgggtgc ggcgtgcccc ggcggtttcc tataccagat cagggcgggt
     1321 gataccttct actcgattgc ccgtgcacat gggacgagtg tggacgccct caggagggcc
     1381 aacccgagag tgaacccgaa tgctttgcga gttggccagc tcatctgcat cccgactgcc
     1441 cttgctccgg gcgtgccctc ggcgcctgcg tgtcctgccg gggcgacgcc ctacatcatc
     1501 cgaagaggcg acacgttcta tggcatcgcc cggcgttttc gagtgagcgt ggcggcccta
     1561 actagcgcca accctggcgt gaaccccggc aggctgcaga ttgggcagtt ggtatgcatt
     1621 cctcctcgca cgggtgggta aggtcaacac ggcgacgcag atcatggtga tcttggcagg
     1681 gctccgctcc ggcggagtct ttctgcttca gagtcgggca ggaggaagcg ggcgacgtcg
     1741 gacgaagata gactatactg caacaagctg aagcatgcag cgaaaacgtg tcatcctata
     1801 ctgggggtga gaggacttgg cgatcgattc gcagctaatc gaggtgagca gctgccttgt
     1861 ggatgactat tcacctctag ttgagtctgg ccagtggcgt gtggctgcga tatgcacgtc
     1921 ggaacaaaac tctgccgatg cgatcatgca gctggaacgg cataatgaga cagacgaagt
     1981 attcgtgttg atgagaggca aggccattct gtttatagcg accggccagg gtgaatgggg
     2041 tactggcaag ataaacaatg tggaaatgga gtcgctgcag gtctacaatg tcaagagggg
     2101 cgtgtggcac gcctgcgctc ttggccccga tgctgttgtt ttggtggtcg agaacagcgg
     2161 cacgggaaga agcaattctg actattctca gctaacgact gaccagcgca ggctggttat
     2221 tgatacatgc gccgggctga ccgggctgac cgggctgacc caagactagc actcggagga
     2281 actgacgtgg cattggcaca aacagaatac atggctaaga gagaaatcgt accgggcagg
     2341 gagctccagc ggcaactccg gcaacggtca atggtattcg cactgctttc agcggcttac
     2401 ttcatgtctt ttgttcagcg agtcgcccta tcggtggtag cggcggaatt ggcggaggag
     2461 cttcacattg gcacggtcga gctggggcta atgtcgtcag cgttcttcgt ggcgtatgcg
     2521 gcagcccagc cggcggtggg catactgtcg gatctgtttg ggccgatgat cgtagtttcc
     2581 tgctgcttgt gcctgggcac aatcggcacg tggatgttcg ccgcctcgcg cagcatgacg
     2641 actgcctttg tcggcaggat actgataggg gccgggcttt caggcatctt catcccaggc
     2701 gtcaagatga taaccatgtt gtacgacgct gacaggtttg catctgttaa cggcttgttt
     2761 cttgccctgg gcaatgccgg cgcggtgctg ggaacggctc ccatggcctg gcttgcttcc
     2821 gctgccggct ggcgtggttc gtttgacatt atcggagtgc tgctaattgt cttgaccgta
     2881 atgtgctggt ttgtgtccag ggcacagaaa tcgcgagtaa cggattgtac gccccgcggc
     2941 gcgggcggga cgcttgcagc ggtcaaggtg tttgcgcgtg accgtaacct ctggctggtc
     3001 agcctgttca tgttcgccag gtatggatcg cagtctgcgt tccagagcct gtgggggatt
     3061 ccacacataa tgagtgcaca ccatgttagc cgtgagcagg cggccgctgc cgtgatgatg
     3121 attggcgttg ggtacatcgt caatgctccc ctagtcggct gggtcgcaga tcgcctgtcg
     3181 cggcgcagcg gcaacgcgtt tcaggcaagg cggtccattc tggcctggac taccggggcc
     3241 tacgcgctaa cctggatccc gatcgtgttc tttccggggc gcctaagcat tggggcgctc
     3301 tatgctctgc ttttcacgat ggggctggga gccagctcag gagggctagg gtttgccatc
     3361 gtgaaggatc agtatcctcc cgaggcggcc ggctcagcca cgtcggctgt caacatcatg
     3421 tcgatcaccg gagcagcagc catcccgcca attgtcggag tgctgataaa tggggccagc
     3481 gtggcagggg cgggaatccg ggcagtgtac gggctttcgc tctggccatg ccttgcagcc
     3541 ggcgtctgtg catggctggc cgccatgctc ttggcggacg ctcctgtctc atgacggcgc
     3601 tccgacccgt cccgcaaatc ctcgaaaata ctcggatatt catacagtac agaagcatat
     3661 tatctactta tctgcttgac ctttgcactt cctaactggt atatttgatc tatacggagc
     3721 acaggcagac gtttgcgttc cacacggcaa tgttacatct ccccgttact gaagatatat
     3781 tcatagactc cgatttgcgc tgcgggctcg tcccctgggg gtacgggctt gacatatcaa
     3841 ctacatcttg agaaatgggg gagagtactg gtgtccatca agaagaagcg cgtcctgatt
     3901 cttgctgcct gtgttgcact ctgccttgga ctgttcgcgt acggaagcgc cgctgagaaa
     3961 gtcgtgaaac tcgggtgggt tgggtctctc accggcgatc aggcgatctg ggggcagtgc
     4021 gagctgaaca cagtcaagat gctggccgag gagctcaacg cggccggcgg attgctcggc
     4081 gccaaagtcg aagtggttgg atatgatcac agaggagacg ccatggagtg cgttaatgcc
     4141 gtgaggcgtc tcaccagcca ggacaaggtc gtgtgcatcc tggggcctaa cgcaagtgga
     4201 cacgcgatcc ccatcgcctc ggtgctcgag gagatgaagg tgcccgacat cgccacggtg
     4261 gcgacgaacc ccaaggtcac tgtggtagac ggcaaagtga agccattcaa cttcagagtg
     4321 tgcttcatcg acccctacca gggagccgtc gcagccggct tcgcctacga tgtactcaag
     4381 cttacgaagg ccgctgttct ctacgatgtg gcggatgatt actcccaggg cctgaccgag
     4441 ttcttcatca agaccttcac tgagaagggc ggcaagatcg tcgccaagga agctttcaag
     4501 tttggtgata ccgacttcag gccccagctt tccaagatca aggcagccaa tcccgagatc
     4561 atcttcatgc cctacttctt caaggaggtg gccctaagcg ccaatcaggc aagagagctt
     4621 ggcattaagg ccactctgat gggcggcgac ggatggccgt cggaggtact gatacagatg
     4681 gccaaggacg cggtgcacgg cagttacgtc gtgaaccacc tcgactatga cgaccccgat
     4741 gttcaggatt tcaagaccag atacacggcc aagtacaagc gagccgtgga gctcaatggc
     4801 tacttggtcc acgatgcgtt cctgatgttc gtggacgccg tcaagagggc caacagcttc
     4861 agcccagctg ccataaggga tgccttggag agcacgaata tcatgggcat aacaggcaga
     4921 atcaagatca accctgcgac gcataaccca gagggcaagg aggcagccat catcaggatc
     4981 atggacggca actacgtgtt ccagcagaag tacgcgccgt gatgatggtt acggtgatgg
     5041 ccatagcatc ctgagcgctt agggcgcgcc ggtgtcgcgc aaggtggcgg gctgccgccg
     5101 cgaaagcgcc tgccggcgcg cctcatgttt cacgtgagat tcggcgtcgg aggggaggtc
     5161 caagatggca cagtttcttc agcagatgat aaacgggttg tctatcggga gcgtttacgc
     5221 gttgatggcc gtcggatact cgctggtcta ctctatcatg agtttcagta acttcgccca
     5281 cggcggtgtg atcatgctgg gatcctattt cgggttctac ctgctcacac tggcgaagtg
     5341 ggcttttccg gcggctttcg tgggcgctgc cctgtgttct gcggctcttg cggtgattgt
     5401 ggagcgagtg gcataccgac ctcttcggtt gcgaaatgcc ccctttctct acttcatcat
     5461 ctctgcgatg ggcgcttcca tgtttctcga gaacttcgtg gtggccacaa taggccccac
     5521 tttcaagact tatccgaccg tgctgccgcg cgcgcccatc gagattggtc ccatctatat
     5581 cggtcgcctc gacatcatga tcttcatcat ctcagcagta tgcctagcgc ttctggtgta
     5641 cttccttgat cgtaccaaag taggaaaggc ggttcaggct acagctcaca atgcgcgagc
     5701 cgcgaaactc atgggaatca atactgactt cgtcattacg ctagtgttcg ccataggcgg
     5761 attcttggcg ggcgtggccg gagtaatgtt cggtatgaaa tacacggtgt acccacagat
     5821 gggaggcata accatcaaat ccttcatcgc ggccgtattc ggaggactcg gaagccttcc
     5881 tggcgcggtc ataggctcgc tgcttctcgg tatcatagag actctcatct ccggctactt
     5941 ctcatcgcag ttcagagact tgatctcgtt cactctgctc atcgcggtgc ttgtcttccg
     6001 gcccatgggc ctcgcgggca aggccaccga ggaaaaggca taggagggac ggctgtgaac
     6061 tggttctata tcaagggaat attgatgctc gcgggtatga acctcatggc cgttctgggc
     6121 ctgtcgctgc tgacaggatt tacgggcctg ttttccttcg gccacgccgg tttcatggct
     6181 ttgggcgcct acgccgcagc ctgggccgga gtgcgcatgg gacttccgtt tctagtggcc
     6241 ttgctcgccg ggggcctatt cgccggcctg gtgagcctgc ctatcggccg gttgactctg
     6301 aatctcaagg gggactactt ctgcattgcc acactgggat ttggcgaagc aatacgattg
     6361 gttctcgaca atgtgcagac gttcgggggc gcacgtgggt ggcctggtat ccccatacgg
     6421 actgatcttt ggaatgtggc gctgatatgc gttgcagcga cattgttcat ggtgcgtctt
     6481 gtcaactcaa ggcacggtcg gaacatgatc gccgttcgcg aagaggagct ggcggccaag
     6541 accgtgggca tcgacacctt caaatacaag atgttatcgc tctttgtgag cgcagtgtac
     6601 gccggaatcg caggaggaat gatgggctac tacaccgggt ttctgcagcc caagatgttc
     6661 gggatggcaa aatccactga gctgaccatc attgtgatct tcggcggcat aggcagcgtc
     6721 tccggaagtg ttctggggtc gatcttgctt accgcgcttc ccgagatact tcggaccttc
     6781 gcgatatggc gactggttgt gtacggcgcg gcagtcattt tcatcatgat cagccgaccc
     6841 gagggactga tgggcggcaa agagataacg ctcgacgcga tccggcgctt cttgtgtggc
     6901 agaaaagccc agactggggc tggggctgga acgggggccg cggcaggggc cgaaggcggg
     6961 gaggtgggcc catgaacatc cttgaaacgc acgacttgac caagactttc ggaggactga
     7021 ccgctgtcga agacgtcagt ttcggtgtgc ggagcggttc gatatccggt ttgatcggcc
     7081 ccaatggcgc cggcaagacc acggtgttca acttgattac aggcatctac aaggttacag
     7141 cggggcaggt cgtgttcgac ggaaagccca tccacaatct tgagccgcac aggatagcgc
     7201 aggcgggcat cacccgcact ttccagaaca tccgtctctt caagcggctg tcggtgtaca
     7261 acaacatcct cacagcctgc catgacttgg ccggctacag cgtggccgat tctgctttca
     7321 ggacacggcg ctacggcgag caggagaagc agctcaagcg cgaagcgtct cgccttatgg
     7381 atgtcatggg attggacgag tggcgtgaca agcaggcggt aagcctgcct tacggggtgc
     7441 agaggcgact cgagattgcc cgggcactgg cagtcaggcc gcgtcttttg ttgctggacg
     7501 agcctgcggc cggcatgaat ccggacgaga ctcgccagtt gatgacgctg atatcgagga
     7561 tacgtgacga gttcggctgc acgatactgg tgatcgagca ccggatggac ctgataatgg
     7621 ggatttgtga gaatattgtg gtgctcaact tcgggcgtaa gctgacagaa ggaccgcctc
     7681 gtgccataca aagcgacccc caggtcatcc aggcgtacct gggcgagggg gtgaagactg
     7741 gtgcttgagg ttcggaacct gcacgttcat tacggcggca tccatgccct ccgtggtgtc
     7801 agtgtgtcgg tgggccaggg cgagatcgtc actatcatcg gcgccaatgg agctggtaag
     7861 tctacgctgc tgaacgccat ttcgggcatt gtgagagcca gcgaaggcgc tatgaccttc
     7921 aagggatcac cgctgcccgc agcgtcttac gccattgtca agcacggcat atgtcaggtt
     7981 cccgagggca gactggtctt cgctaatttg accgtactcg ataacctgat gctcggagca
     8041 tacctgcgcc acgatcatca ggcgatccgg aaagacctcg accgcgtcta tgagttgttc
     8101 cccaggctcc gggagcgtgc gagccagatg gccggcacgc tctcaggcgg tgagcagcag
     8161 atgcttacca ttggccgagg tctcatgagc gacccggagc taatgctcct agatgaaccg
     8221 tcacttgggc ttgcgcccat actggtggag acgatattcc aggtgatcga agacatcagg
     8281 agggcaggca agacgctcct actggtcgag cagaacgccc ataaagccct ttccgccgcg
     8341 gatcgcgctt acgtgttgga gcatgggcag atcgtgaaat cgggtaacgc tcgcgagctc
     8401 ttgagcgacc cgtcgatcca agagtcatat ctgggggtga agcagtagcc cgcccaggag
     8461 gaatccgtcc tgcgagaatg gaactcttaa gagacagccg cgcaggcgcg tcagccgcgt
     8521 gggctgcggg cgcgagtgaa ccgggaaggg cggacaaggg gattgagacg tgaagataga
     8581 gacggtcgca ggtcgaatgc ggatttgggg aatgggcgag gattaggcgg aggcggccgc
     8641 ggtgccgaac cggcgtgtgc ccagtcgttg ccgcaatacg aggggttttt cgcggaattc
     8701 tatgacatac tgcattcatc gtacgacgcg gacctcaaga tgtacctgga cctagcgaga
     8761 gcgcacggtg atccagtgct ggagctggga tgcggcacag gaaggctctt gattccgttg
     8821 gccgcggcgg ggcatacggt gaccggaatc gacctctccc gcgacatgct gacgcggtgt
     8881 caggggaagc tcgacatcga gggaaggtca gtagcagggc gagcctcgct gattcagggt
     8941 gatatccgcc gattcgagct tggccgcagg ttcaacctga taatggctgc ctgcaacacg
     9001 atactccact gcactgcctc gcaggatctg ctggctgtgt tcgcaagagt gcgagagcat
     9061 ctggcgcctg gaggcgtgtt cgttgttgac ttctccatac cgaatgtcaa ggccatgatc
     9121 gagtccagcg gggaggaaga ggtattcgag gtcatccacc cggtccgcgg cagcaggata
     9181 gtcgacacct acaaggccac ctttgatttc gccaggcaga tcgagaccat cgacactcgc
     9241 ctggaggaat gggatggtgg ctcgctagtt aggtgggccc gagcccagtc agagcgcggc
     9301 atatacttcc cgcgggaggt agcgctcgct ctgacgtgca gcgggttcag gatcgcaaag
     9361 acgtggaagg gctatcgtcg cggtccacta gtcgaaacaa cccaggatat agtatacatc
     9421 tgccgggtcg atgcctcccc gtcgccctcg gagcgcccgc aatagccgcg tgacttgttc
     9481 ctgtgcgctc aaagcagagg tcaccttcgt gaccactaca gacgttgggg tgaatgtgaa
     9541 ccacttgctc tcaggggtta ctatcacgga accttgcagt ccgccttggt tccacgcccc
     9601 ggtttcatca gtgactgcgg gctatggcac cgaactcagc cttacagaaa caccgctggc
     9661 gccgactccg ttgagggaga ggactctccc acctgcgcag aacaccatgt actccgccac
     9721 acaatcggcg gcggtatcgg ggccattcaa cacatgatgt gagggcgaaa accgccagcc
     9781 atctctggag aatgttgcgg tgacctggcc gttgtgatgc ggcccgagaa tgcgggcgca
     9841 gccagcgtag gtcttccgca tagacgaatt cctcgcaggc ggaacaaggc tggcccgggg
     9901 cttggctgag ccggaaggac ggcctgcctt gtgaatacct ggcgactgca caaggctcct
     9961 gaggagaatc cggcgcatgg ccatactccc ccattcggtt acatcgtcaa ggcttctggg
    10021 agcatgtttt gttctgtgtg tcgccgacct tctgcgaaaa atcgatttca ttccccaacg
    10081 tctgtgcgcc tggtagttac catagtttgg accattctgc tcgttttgtg gccggttttc
    10141 tgagcgacct gctcggattc tgccaggttt ggccggcact agttgtctga attcgcgccg
    10201 gcaacggcgc ctcctttttc tggagtcagg caccccgcaa atccgcaggt agggacgtat
    10261 tacataatat ggctatacgc gcacccctcc cgaatgcgtc tgaaaaggcc tcaaatgccc
    10321 accgatccac cagtaaaacc caaattggcc cgccctcgcc ccctcccggt gttcaaacct
    10381 gacacatctt gagcaggttc gtcagaaaac cagccacatt tcttacaggt tcgctagttc
    10441 tctggcatac gcgggatcaa accttaaccc aaggcgcatc atactgcccc cgccccaagc
    10501 tgcaggcagc aacccggtcg cggcgactgc ctagagcttc gtctgtaccg aagctgagtg
    10561 gtgcgactgc tccacgcttt cgcccgcatt tcttgaagga atccccgtat ggttggagaa
    10621 tccgaaaatg tacgtggccg gcagcgtttc caatagtctg ctacgtatgt tgatagctac
    10681 aggccccgga gccaattatc gtcacactaa cgaacgctgc ggcacgtctt gctagaccga
    10741 atgcgaatgg aggttggcgg tacgcacatg gaacacggta gcgcgctgtg cttggcgccc
    10801 gtcatgttgg acggcgtgga ttacgataac cactcatggg ctgcgcgaat gcaggcttgg
    10861 cttgcatccc ttccgtacga tgctcagctg cgggcagggt cattttgcga actgatgcgc
    10921 gaagcgttcg atgccgaccg tgccgggatg tacctgaaga gtctctccat tctcggtcgg
    10981 gccgagcgct tattggccga ttgcgactca ctcgattcct tcatttacgc gatggggggg
    11041 cactccacgc cggattccaa ctgatcccgg aggcggccca cggaacacgc aacccggccc
    11101 taccggggga ggcggcacga gctcgggagg ttcgtgcgat tccatggatg acgacacgtg
    11161 ttcgcagtgc atcggcggcc tatgccttta cagtattctg aatgcaatat gcggaggcgg
    11221 atgctcatag gccgtttcgg acctgccgcc agttcgctat aggaaggaga atcattgtgg
    11281 agttgcccga ttcggttccg gtaacgcagg atttcagtgg gcccattccc ggccctgatg
    11341 ttggacccga tccagatctc gcatccgagc tagggcgaga gcacatgccc gaaccccagc
    11401 tcaaacctga acccgaacct ggcccaggcc ggcctaccag ccatgctggc attgcggcag
    11461 agatcgcggc agcacagggc aaattggacc agcttatcgc catgttcgcg gacaagatcc
    11521 agtacgacga tcagaaggaa aaggccttct ccaggctcta cgatgagctt gactccttta
    11581 agcgcgactt tgtggatcgc cggatgaagg aggcctatac cgacctgatt ctactctacg
    11641 acaacatcaa gaactatacc ggcgatgacg acgttatgaa ggaagagggc gcgctcgcgc
    11701 gaaggtacct tggctatgtc atggaagaac tcaccgaggt gctggcgcgt cgcgggatag
    11761 aacctatttt cgcaggccca ggcgtgagcc cgaacgcagg tagttttgat ccgcagtttc
    11821 aacgggccat acgcacggtg cctgccgaat ctcgcgacga ggatgggcgc gttgtccgcg
    11881 tagtgaagga cgggtttaca aaggacggta aggtgctgcg cccgcaatcg gtgatcgtgg
    11941 cccgttggac ggaaccgccg ggccccgcag gaggcggtgt gtgatgtctc gcgcaattgg
    12001 aatcgatctt ggcactacat tctcggccat cgccgtactg aacgagtttg ggaaggccga
    12061 gatagtcccg aatgcagaag gagaacgtgt cactccctca gtcgtgtact ttggcgatga
    12121 tgattgtgtg gtagtcggga gcgtcgccaa gaactctgct gtgagcgatc cgcataacgt
    12181 ggtggagttc atcaaacggc agatgggcaa tgccgattac gcgttcaccc acggcgatac
    12241 cgaatacggc cctgaacaga tttcggcgct tatactcaga aagctcaagc aagacgctga
    12301 aaggctgctc ggcgaggcgg tcaacgatgc cgtgatcacg gtacccgcct acttcgacga
    12361 cacgcgcagg cgcgccacga taaacgccgg caggatagct gggctgaacg tgcttcgcat
    12421 tatcaatgaa cccacagcgg cggccctggc ctacgggctc acgaatgcgt ccgaccagcg
    12481 gataatggtg tatgacctgg gaggcggcac attcgatgtg accatcatgg acgtcggtcg
    12541 cggcgagatc acggtcgtcg ctactgatgg cgaccacatg ctgggcggca atgacttcga
    12601 cgaccagata atgctttacg tcaacgatgc cttcaagaga gagcacggag tcgacctgct
    12661 ggcggaggta gaagtacagc aagacctgcg ccagcgcgcg gaggccgcca agaagacgtt
    12721 gtcttcccgc ccatcggcta agatcagcat gagcgcatcc ggcaagcggc tgacggtcga
    12781 acttacgcgg gagcagtttg aacagatgac cggtcacctt ctcgagcgca ccaagatgct
    12841 tgtagagtcg gtgatgagcg aggccaaaac gggatggggc cagattgata cagtgctctt
    12901 ggtaggagga tccacgcgca tgcccatggt gcgccagatg atcgagcgca tgtcgggcaa
    12961 agccccgagc caggccatta accctgacga ggccgtggcc atgggcgcag cggtccaggc
    13021 cggcatcctc atggctcaaa agggtgatgc ctcgatactt gcgaccgccg aagggcgccg
    13081 gcttgccaat acacgagtga ctgatgtgac gagccacgcg ttcgggatcg tgttgatgga
    13141 cgatcgcacg aaaaccgagt acaaccaggt gatgatcgcc aggaacacac cgatccccca
    13201 cgaggtgcaa gatcatttct acaccatatc caacaaccag gcgcgcgtta agttcgtagt
    13261 gctgcagggg caggaccgcg atccggctaa ctgcaacacc atcggcgaga ctgaacttga
    13321 gttcgatagg cccaaaccga ggggctatcc tgtgcgggtg acgtaccagt acgacgccaa
    13381 catggtgatg cactgctata tggaagacga aaggactggc cgcattgccc agctgcacat
    13441 atcgcagtcc ggcaggatga gcgaccgcga ggtcgaggca aagcggcggc tgatggatcg
    13501 cgtaaaggta gactgagcag gagagcaaag gagcagagga gcagaggagc tggcgatggc
    13561 aaagggcgct atacatagag tctgggcgga ctacgacgcg acctacggcg ggcgacgggg
    13621 catcatgatt catgcctcaa tggcggtcac cgaacgcaga agcgctcagt cagagcaagt
    13681 attactgatc tattggttct actacaccga cggcaagccg gtgcccggcg cttctggcga
    13741 gttcaccgac agcggcggga acgcctgcgt ctatgagggt gtcgacactc cgtacccctc
    13801 taccgagtac gacgatttct cggtgttcgt cccctacgag gcgcttagcc accaggggtc
    13861 cggatcgttc agcgcgtacg ccaacgctgc cctgttcgaa ggcaactctg tcatagcctg
    13921 ggcgccgagg gtcctgtcgt tcaccttaac catccgggac gttcatggcc cgcaaccgca
    13981 cgggcagccg cggcccaaga gcactcccaa gactacgacg gcaactaccc cgaatacggg
    14041 cggctcaggg ggaggcgggc ggtcgttcga tagccaaacc cctgcgcaga gataccgcca
    14101 gatgttcggc ataccgcccg gggccagccc ggctcagata aagcagattc tggaccgcga
    14161 gtatcagaag tatagagcgc gggtgaatag ccctgacctt tcggcaaggc aggaagccga
    14221 ccggatgatt atggagatag gcaaggcccg gcgggtgctg ctgccgcagt agaaggcggg
    14281 caaacgacca agcgagcgga ggtgtaccta tgaaggcagt tggaatcgac cttggcacta
    14341 cgttttcggc tctggccgtg ctcaacgatt acggcaatcc cgaaatcgtg ccgaacaggg
    14401 agggcgagcg aatcactcca tcggtggtcc tgttcgacgc ggagaaccag gtcactgtgg
    14461 gggcgattgc caagaacacc gcggtaagcg actcggagaa tgtggtcgag ttcgtcaaaa
    14521 ggcagatggg caaccccgat ttcgtcttca cccatagcgg cagggagttt cgccctgaag
    14581 atatctccgc gatgatcata aagaagctga aaaccgatgc cgagacactt ctcggcgccg
    14641 agcttcccag cgcagtgata acagtgccgg cctatttcga tgacgctcgc aggcaggcca
    14701 ctatgaatgc cggcaagata gccgggttca acgtgctggg aatagtgaac gaacctacag
    14761 ccgcggcgct ggccttcggc atgagcaacc ccggcattca tcagcggatc ctcgtgtacg
    14821 acctcggcgg cggcacgttt gacgtcacgc tcatggaggt ggcggagggc aaggtccgcg
    14881 tagtggccac tgatggcgac catcagctgg gtggcaagga ctttgacgac aaggtaatca
    14941 tgttctgcaa tgacgagttc aagcgagagc acggcataga ccttcttgac gaccttgagg
    15001 ctctccagga cttgcgccag cgagccgagt ctgccaagaa gaccctttcg tccagggatt
    15061 cggccaagat cactatcagt gcccagggag ccaggctgac ggttgaaatc acccgcaagc
    15121 agttcgagga gatgacggcg gacctgcttg tgcgttcgga aatgctcctg gatgcggtga
    15181 tgagtgcgtc agggctggag tgggctgatg tcgatcaggt gctgttggtc ggcggcatga
    15241 ctcgcatgcc cgcagctaac gagatgatcc gcagagtgac tggcaaggaa cccactctat
    15301 cagtcaaccc agatgaggca gtcgccctgg gggcggccat atacgcaggc atcatactgg
    15361 ccggccgggg cgatcccgag gtagtgttga gcgaagccgg gcgccgattg cgcgacacca
    15421 ggatcgaaga cgtcacaagc cacagctacg gcgtgctgct tgtcgactcc aagacgggcg
    15481 acgacatcaa tgagataatg atcgccaaga actcgcccat ccctgcgtcg cacaccgcca
    15541 cttactacgt acatgaagac ggtcagtcgt ctgtggaact cgtagtgctg cagggcgaag
    15601 acagaaaccc ggccaactgc aacgagattg gcagggtggt gttgccgctg ggcggctcga
    15661 aaccccgcaa ttatcccata gacgtaacct atgagtacga cgccaacatg ataatgcatg
    15721 cgaccatcaa ggatccgcag acgggggcct caaagtcgct ccagatcacg cagcagggac
    15781 ggctgactaa tgcagaagtc gaggaaaagc gggagaccat gcaagaagtc gaggtagtgt
    15841 aatggaggcg gacgcagagc ggggcgcacg tacccgggcg ctttatgagc aactgctggc
    15901 acccgtagcc cgtcaggtcg acgcaaggct ggccgatgaa gcgcacaacc ttccggctga
    15961 actgcgcgac atgtatgact gcgcggtcgg cgggggcatg agatttaggc cgtgcctgat
    16021 gtacctggcg tacctggtgt gcagtgcagg cgtggacggg gaaggcgggc gcggcggcgt
    16081 cgagggctgt ggcagccgcg ttggcatcga tagtctgatt cagcttgcgt cagctgtgga
    16141 gttgctccac aaagcgtctt tggttcacga cgacctcatc gacggcgacg ctcttcgtag
    16201 gggtcagcag tcgtttcacg cgacgttcgg gagcgagcgt gccgtagtaa tgggcgactt
    16261 actagtggcg atggcccacg acgtggctga gcggatgcgt ccggtcttgc cggctggagt
    16321 gtactgccaa atccgcttcc atttcaacaa ggcccatctc gacctatgcc gaggcgagtt
    16381 gctggagttg gtcgctgcgc gtcgcgagcg ccccctggca cgcgactatg tggatagagt
    16441 gatcgaagga aagaccgcgt cgctgatgga gcagtcgatg gctatggggg ccatcatggc
    16501 gggcgcgccc gcggcttgcg tggatgcctt ggcacgctat ggacggtaca tggggttcgt
    16561 gttccagact atcaacgata ttaacaacct gacagggttc gacctggaca tcaaggggca
    16621 ggccatgaca gacctggcgc tgggccgcgt gggataccat gtcctgggcc ttgagatgac
    16681 ggcaggcatg gagcaggtgg gcctgctggc gtcggagatc aagcgggttg aacgtgccga
    16741 cgatggcctg cgtctgcgca agctggttca gaaggggcgc ttgcgcatat ggctcgacgg
    16801 ggtaatatgc gaatatgccg accaagcccg acaggcgctg aaagccttgc cggagtgcgg
    16861 cgcgcgcgac gctttggact cgataggccg cgagatgttc gaatcgtggt tctggaatcc
    16921 cgaatcgagc atcgactgcc tgggaggcgg atgcagtgag aagtgctcgc cctctgactg
    16981 agtcggctct tgccgcggca gtgtgtgtag tcgtatgggc gctgtcgatg ggccttccgg
    17041 ggctcggagg ctatgtgaga tacatgacgc ccagcttaat cgccatcgtg ggcgcgcggc
    17101 atggagcacg atggtcgctg gcggcgtgcg cggtgaccgg cgccggcata gggatcacgc
    17161 ttgggccgac tcagctccta atcagccttc ttctgggcgc tcccacagcc gtaatgcttc
    17221 cactgatgaa gcagcgcgtg gcgcccgcgc tctggtgggg gctggcgtca gctgtctacg
    17281 ctgtggccgt taccggtctg ggctgcggtc tggccgcgct ctacggcgca gggcctcgcg
    17341 actacgtgtc ggcgactgcg tcggcaaact acgctgctct gggcggccta tgggcttggc
    17401 ttggcgtggg gccgggcgaa ctagccgcgt ttgcaggccg gtctgcgtgg ttcatcctcc
    17461 cgggagtggg cgtcgtctcc ggagtcatga tgtacctcat taacgtagtc ccgtacagaa
    17521 taggcctcag aaccctcgac cggacgcgcc gtcgccggta gcgccggcgt gtgcgctccg
    17581 ggtgctagcc gagcccttcg cctgcgcaag tgcagcgagg tctacatcag cagaggatac
    17641 tcgaaccacg gcttgcccag gctgaatgct tatgcccgcc atgtccagcg acttcagcgc
    17701 tccacggctt gcttgaacta ggtcgatggt gattacgccg cggcccaggt agctcacgcc
    17761 ctgaggcaga cgcggggcga tcctgttgat gacgacatct agggggcctg cccccaaccc
    17821 catcgggccg gaaatgcgca ccgtcaagct ggccccgcgc gcggcgacca cctctacccg
    17881 gaaagacgga tgaatgggca caggcgcgcg aacggtagta tcgacaacga tcaccccttg
    17941 ttcgaacctc acacccctca caacgaattc cgcggacgat gcgatgcccg acacaacctg
    18001 cttcgcttct cggtcatcta cagcaagcac cagcgactcc attagcatct cgagttcact
    18061 cctttttggt tctcgcctga agcaggcgtc ttaccctgat atccagcccg agcaggatga
    18121 tgttctcaat gatggggcgg tagcgattca gcgtcagctg cgatagcgcg tggcgtgctt
    18181 ccacattgca cctggcgatc atatacgaga gagcgtctgc tgccccgggt gaaagctcag
    18241 cagaagtctg atccaccatc atgcaggcgc tcagaccgtt gaaggcgccg cgttttgcgt
    18301 ctcgcgctac gtcctccagg cagtcgccca gatagaccag gcgacccacg gccttgccca
    18361 ggcgcgccag tcccgcacag ctctcatctc gcccagccaa agcgcccgta tactcaaaca
    18421 cgtgcgcgag gccatctgcg gtaggcgcgg tgagttcgtc aaacgtgatg cttcctggcg
    18481 ctgcgcttgc ctcagcttct agctcggcca ggcgatcgtc tgcctgcagc agcggggcga
    18541 tatcgaagga gtgttcggca agcacacgcc tggccctgct gaactgccga ccgcctatgt
    18601 ctctggccgg gcgagctaga agcgacccat cgcgcaccgc atctaggctc ttcagctcgg
    18661 ccatgcacac tgtgaacgcc gcgcctagcg tcatgaagcc ctcatgctcg ctgacaatag
    18721 cgaacctgcg gaatgccgac acagggcaga tagtctttcg aaaagacggg tctgcgtctg
    18781 cctgagccga catgagcaag accagcagag tagactcgaa attggtgagc gcgcgccaca
    18841 atgtgccgta gtctcgtgca agtgcgaaac agagcccaca gtagtgggct gtgtagtgat
    18901 ggccttgcct gagtgtgagg tcgagcttct gaggccttgc atatccaaac aaccgggcac
    18961 tctcctgccg acgtaatccg cttcctatcg ggagttttct ccttgccggg cgcttgttcc
    19021 tccataggcg gcgtaatccg ggacacagag cgaggatcgc cggttgagtg ctgcaggacc
    19081 ctgtcggcgg agtggcgaat tcgattgcga agcgagtgga ttagggtaac agtggcagag
    19141 agagggagga accgcatgcg cagaatcatc atggtcttgg gggtcatagt gatcgtcctg
    19201 cttgcgggat tcgcggggtt cctaggcttc atttcgatca ccgactaccg tcccaaggac
    19261 gttatccggg tcgcagtgga taatccccag ggggaggtca ttcctgtcgg gcgggagatc
    19321 acgataacgt ccttcaacac cgggtactgc ggcctagata agcatgccga cttcttcatg
    19381 gacggtggga ccatgtccag ggcagttagc cgggaacaga ccagcgctaa catcgaccgg
    19441 atctcgtctt tccttatggg gcaggattct gacatctacc tccttcagga agtggaccgc
    19501 aaatcgcgcc ggagctatgg caccgatcag taccggcgct tctgcgcgga tctagcaggc
    19561 tacaacagta gtttcgctac gaactacaag gtggcctggg ttccggttcc tctggccgac
    19621 ccgcacggta atgtctactc aggcattgct acgctttcga gataccgcgt tgacggcgcc
    19681 gcgaggtact cgctcccggg cgagtttacc tggcccatgc gtctttttga gctggatcgg
    19741 tgcatgatgg agagcaggct gcccacaggc gacggcagac aactggtgat tgcgaacgtg
    19801 catttatcgg cgttcgacaa gggcggattc atcaggactc agcaattggc ctttctgaag
    19861 gagtatgcgg cgcgtgagct ggagtcaggc aactatgttg tgataggcgg agattggaac
    19921 catttgctct cttctgaccc cgaggccaag aaggctgaat acggtgagtc gtggcccttc
    19981 tggctgcaag tcctgcctga tgatttcgtc attgatgggt tcagatgggc ggtcgacgag
    20041 acgacgccgt cgtgccgaac actcgacaag ccctacgttc cgggtgagaa catcgaagtc
    20101 aacattgatg ggttcctcgt gtcgccgggc gtggaggtcg tacatgtgca cgggcatgat
    20161 ctcggtttcg aaaacagcga ccataacccg gttaccgttt gtgtcaggtt tggggaggaa
    20221 gtgccgggtc aggactagca gtccagccag gaggtttggg gcacaccaag gtcgtgccgg
    20281 gagatatctt cctcggatcc gttatcccgt tcatggccag tatctctcgc atcctctcat
    20341 cagtagccct tccatagagt cgtttggcga tcacagctac tgattcgccc ggcgctaccg
    20401 tgactattac agtgcctggc tcgccaggtc tgacagcgac cctcggggcg ttgccatggg
    20461 tagcaacctg ggcctgttgg gcctgggatt cggcctgggc ctgctggggt tcgggttctg
    20521 gagcagcctg cggttgagcc tgcggttcag cctgcggctg aatcgacggc tcaatttcaa
    20581 cctgaagctc agcctgaagc tgaggccgga gctccggctg cggttctgac gcgagccgtt
    20641 gtgttgtgtt gcgcggcgcc cagatctgcg ctgtcagggc tattactgcg atgacgaggg
    20701 ccgcgaaagc gatgcgcagg gccagccggg tgtcgtcgct tctaagcttg aacggccaac
    20761 acaccctgcg tgacgcctga gcagcagcgg gcccgcgtag cgactgccct gaggattcgt
    20821 cgatggaatc gaacatgtag taccccggga agggcagcag cgcttcttcg
//
LOCUS       DAOTAC010000005        19294 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_2333_length_19294_cov_4.867145, whole genome shotgun sequence.
ACCESSION   DAOTAC010000005 DAOTAC010000000
VERSION     DAOTAC010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 19294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19294
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2333_length_19294_cov_4.867145"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            47..685
                     /gene="pncA"
                     /locus_tag="PLM74_00475"
     CDS             47..685
                     /gene="pncA"
                     /locus_tag="PLM74_00475"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HON41591.1"
                     /translation="MKTLILVDLQNDFMPGGALAVPGGDEVLPIANAVQGCFGIVVAT
                     ADWHPPGHGSFASAHPGARAGQLVELAGMPQVLWPDHCVQGTGGAEFAPGLDTARIAK
                     IIHKGMDPSIDSYSGFFDNGHRRSTGLEGYLRSRGVDAIYVMGLATDYCVKFTALDGV
                     ALGFSTYLIEDGCRGVEVAPGDVTRAVSEMRTAGVRIINSRQLGVSGGAPRE"
     gene            735..1952
                     /locus_tag="PLM74_00480"
     CDS             735..1952
                     /locus_tag="PLM74_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HON41592.1"
                     /translation="MRFKGLVIGVPKEIMKGERRVSAVPDTVKKMVAEGAKVLVEKGA
                     GEGSYFHDHQYAEAGAEIIESAADVFAKANIILKVKEPIFNEAIGKHEVDLMHAGQCI
                     ITFLHPAYPGNHKMIKDMSAKGILALTLDGIPRISRAQAMDALTSMSTVAGYKAVLMA
                     ANRLPKFMPMVGTAVGMIKPSNVLVIGTGVAGLQAVATAKRLGAVVYAADIRPAASEQ
                     AKSVGAKVVDLGVPADLAIGEGGYALKLPEEWLLKEREVLAPIVADADIVVSAALIPG
                     KLAPILITEEMVKSMRPGSAIVDISIDQGGNCELSESGVILEKYGVSIDGTKNIPGMV
                     PTASTWMFAQNIFNYVANLVKDGKIDVDMSDEIIASSLVSRDGVLVHAGALEAMNAVD
                     GGNKKCGCNCGSK"
     gene            1984..2268
                     /locus_tag="PLM74_00485"
     CDS             1984..2268
                     /locus_tag="PLM74_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007365250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HON41593.1"
                     /translation="MSPLSLVILFVIATIVGYKVISAVPSLLHTPLMSGTNALSGVTV
                     LGALAVTAMAQTMGNSAMGQLLGAVAIVLAMINVVGGFLVTDRMLRMFKK"
     gene            2331..4379
                     /locus_tag="PLM74_00490"
     CDS             2331..4379
                     /locus_tag="PLM74_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010956284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HON41594.1"
                     /translation="MSWKEAIFLGLGLADILGLICVIGTVWGIKMMSSPTTAVNGNLL
                     GAASMAGAIIVTLVSEGIVGIGILWVAMAVGTVLGYVMAIKVAMIEMPQMVALLNGFG
                     GGSSAIVSLMTLVATVWTAGTVTFGAFEKVTAVLGLIVGGVTFSGSLVAAGKLAAKIN
                     QRPIIFERQSAINNLALIVTMVLGVSAIVSDGTGMVVYAVLVLIGSLAYGVLFTIKVG
                     GADMPITVSLLNSFSGVAASISGFAIGNPLLIAIGAVVGASGLILTQIMCRAMNRTLG
                     QVLAGKTTVLHPTHGQKAAAGSAGPSRPVQPSGAPAQAPPTAAEPAPAGEPNYIGIAW
                     SAKSVIVVPGYGMALAQAQGDVKALMDRLEAGGAEVKVAIHPVAGRMPGHMNVLLAEV
                     DVPYDKLYEMDQVNDEFAKTDLVIVVGANDVINPAANTAEGTPIYGMPILNVHEAKHV
                     IIFNYDTKPGYAGVDNPLYEPSDKVTLLLGDAATTVRKFMGDIEATATATASAGGSAE
                     PAESKAELVQITREARSVIVVPGYGMALAQAQGDVKALMDRLEAGGAEVKVAIHPVAG
                     RMPGHMNVLLAEVDVPYDKLYEMDQVNDEFAQTDLAVVVGANDVINPAANTAEGTPIY
                     GMPILNVHEAKHVIIFNYDTKPGYAGVDNPLYEPSDKVTLILGDAAKTVREFMNEVGL
                     "
     gene            complement(4573..6423)
                     /locus_tag="PLM74_00495"
     CDS             complement(4573..6423)
                     /locus_tag="PLM74_00495"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HON41595.1"
                     /translation="MLIRTNGKTVMTCGPRVVRAAFIVAAAIAAVIWLGPAALATAEQ
                     QSPYARQGADAAIVDSGYTRHYASLNAWDYRSEFVDVNWLVSTLARAGISAAVITDHD
                     LEIGALRSYKAVVLPSTSCISQLAAENIRKFAEAGGRVFATFDTSLRDDFWRVHPDYE
                     LTEIMGARYSSTEKVSADPLIASAGSHPIFRGLGSTIPVPRQRGLVNRVNHDALVLAK
                     WSDGRPAMTETRYGVYCSENLFSAANATTPDVATLITNTIAYLVNVKYGAQFNLGQLK
                     TKAAWYTPPTDRERITHDLKRLAAAGFNTLFVSAFSQGQALYPSQVAERNPVYGDFDP
                     LQACIQEGKLLGFSVHAWVEAFDVGQAATDGSLPRLLQANRGWAAMKRDDRGPTAAPD
                     GHYFLSPAHPEVQQFIMDIARELVTCYAPNGIHLGSICYPAGPAAPYDFNPTVLQLAQ
                     ADLGFAPRSIGLNIARWNAWLDWRCSNLSAFVGRLVSTIRAESPGTLVSASVYPYSDS
                     VLVRMQDWSKWAAAGWLDFVVPLTNTRVADLVDTLVRAAQGFAGTKIPVVAGIDAASI
                     PKAKSSAPALAGLVDAARAAGAYGVSVDALAVSDEALSALLNGPFRTPAE"
     gene            6567..8210
                     /locus_tag="PLM74_00500"
     CDS             6567..8210
                     /locus_tag="PLM74_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017436843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HON41596.1"
                     /translation="MEYYDYTQPFASRRSPVMGMRGAVATSHPLAAQAGLRMLLAGGN
                     AVDAAIATAAALVVLEPTSCGLGGDAFAIVWDNGEMHGLNASGRAPQALSAEELRRAG
                     FANMPAEGWAPVTVPGVVSAWSALSRRFGCLPFRDVMAPAVEYAREGAPVPPVIAGYW
                     SAAQKRFGGRGGVFAEFEHAFLLGGRAPRPGEVYRNSELAMTLQSIADSHGESFYRGG
                     LARAIAAHSEATGGYLALRDLAEHLPEWVEPVNARYRGFDVWEIPPNGQGIVALMALN
                     IMEGFDLGDMPRLAPELLHVIIEALRLAFADAHAYVGDPRAVSVPVCELLSPEYAAAR
                     RAQISMSQAMADVAPGRLSGKLETWEPHDGDTVYLSTADWEGMMVSYIQSNYMGFGSG
                     VVVPGTGISMQNRGAGFSLEEGHPNELAPGRRPYHTIIPGFLTQSGVPLAAFGVMGGD
                     MQPQGHVQVISSIIDHSLNPQAAIDCPRVRVMGADGVLAEPEVGADTIAALRRLGHNI
                     RAEEGRAGFGGGQMVWRDPDTGVLIAGSEPRKDGCAVAI"
     gene            8258..9118
                     /locus_tag="PLM74_00505"
     CDS             8258..9118
                     /locus_tag="PLM74_00505"
                     /inference="COORDINATES: protein motif:HMM:NF021443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LiaF-related protein"
                     /protein_id="HON41597.1"
                     /translation="MRGSIGASMVFGIVLVLLGAMFLLNNLGITTISIGRIIETYWPI
                     VLVALGLSAVLKPRDDGRVRDERTYDDQIHDRPTALYDEAGSGNEEAEPAGPREIGEA
                     PEPCRASGPNVGGWILIGLGVLMLAANTRFWGFDLSRLWGTFWALVVILVGWTVLRSS
                     PPVGQVGRTQWVVMSGLEQRAAGWPLRSGSYVVVMGGADLDLRVADVLDGETYLDMTA
                     IMGAIEVIVPPDISVECEGAAILGGVTVLGQSSGGVLASRSFVREAPAGSTKRLRVRC
                     RAILGGVEFK"
     regulatory      9210..9350
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            9342..10661
                     /locus_tag="PLM74_00510"
     CDS             9342..10661
                     /locus_tag="PLM74_00510"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HON41598.1"
                     /translation="MFRICTPQARGVHRRRVLRRSALALAAFAFVFAVTTVLPVGRVE
                     ALFGRSGLSFAVVGDSQGRNEVWDRVIDAVNASRPQFVLHCGDMVASGTSQQYDDFVG
                     RARKLRMPWYAVLGNHDVKGDGKAVYARLVRKDAYYSFVQKGYRFIGLDSSSGSLDAT
                     QIDWLRRELQAPEPKFVFMHIPLFDPMPGGDHCFLDTGQAAELHKMFSTSRVLAVFSG
                     HVHVFAQAAKDGVTYVTTGGGGALLYARAEQGGFHHFTMVRADGDRIQVEAVPVKAEI
                     EEPRLTVRGSKGQVEMTLGEIAAMPRFEAKTAFENKFANLGGQGVYAGVRVAALVDMV
                     GGMQPGQILVVTSSDGYSQEYAYGNVYPSVAWLSLQGEMLLAYEKDGSTHPAWGECPR
                     IVFAPSDGVYHNSDCAATSAPGQGWNVYESAGARWARYVRRIEVRTK"
     gene            10680..11660
                     /locus_tag="PLM74_00515"
     CDS             10680..11660
                     /locus_tag="PLM74_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41599.1"
                     /translation="MKKNNALRVLAIVAFACLVLGMGRLAAAGEWQVEVVSGDKSVVV
                     TAADAAPQAQTVKAGFLRSTGRIEGPSAYAVMPMDAILARVGGIRPEQAVRVTARDGY
                     QMVYSFAQVQGGIFTYDDKGNVLRVGGPSMLLAFESDRDSADKLPRIIYGRPDDIVIT
                     DGHFWAKSVAKVEVIPGVEDWQISLSGVEKTWLDRATFESIVTCPVTPHPAVTWSVAE
                     KDGSTSTYEGAPLWVILSIIDGGDAPGGHYMFNDDLAEKGYTIKVMSKDGLAAEFDSK
                     SVARNNDIIVAYLKNGEYLTEGDAPLRLVGDVPTKKHSVKAIAAIEIVGN"
     gene            11662..12735
                     /locus_tag="PLM74_00520"
     CDS             11662..12735
                     /locus_tag="PLM74_00520"
                     /inference="COORDINATES: protein motif:HMM:NF021348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HON41600.1"
                     /translation="MSSRLAKRRRGYYFSTRDLVLIAAVSAAGGVLSTYVGYLGNLLN
                     SAFGVPFGAGQFMAGLHVFWFVLARAIVGRSGAGALAGVLKGLVELFTGSTHGLPIVV
                     VSAVEGVLVDLLSLPFVRPPLGAMCLAGAVSSASNVLVFQAMYFSGVSWGYVGLMVLF
                     AAISGAVFGGYFTAGVLDVVETAGLLRPVEMSTPGSAPGRRPRPGRRVARLAMTLVMI
                     AALAGGAVYYYTQVFEPVPSSPRCKIEGKVARAYEYAPSGFRDEVVSVRAELVGQVTY
                     VPEADYSGVPLAVILGRAGVDPSAASVRIIASDGYEAIFSVRDITEHTNVLLCQDGET
                     LRIVAPGFEGAFWVRMVSRIRVE"
     gene            12728..14200
                     /locus_tag="PLM74_00525"
     CDS             12728..14200
                     /locus_tag="PLM74_00525"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON41601.1"
                     /translation="MSREMVELSRFGVDYPQGTSPALAQVDLSIGPGESVLVTGPSGS
                     GKSTLALCLTGVIPNAVYARLSGGIRVCGVEPAAQGVYDMASRVGLVQQDAEAQFCTM
                     SVEDEVAFGPENLMCSEAEIERRVAHSLELAGAEHLAGRALSELSGGEKQRVAIASVL
                     AMQPQLLVLDEPAAHLDPRATRAVADSLHVVQRDLGLSVIIMEHKPWRFAGSTRRMVH
                     LDEGRVVHDGPVKWDRPGHLHSPVARSSQPRPGGPLLAVRGLSHAYGKRTALADVHLD
                     VARGEIVGIMGDNGSGKTSLLLSIMGIVRPQAGRVTLRGEDITALPVSARASRMGFVF
                     QNPNHQLFTDSVWREVAAGPLARGESAEECRPATMSLLQRFGLADMCDRHPLTLSFGQ
                     KRRLNLAAALICDPDLLMLDEPFAGQDMRRAEDLACLLAEVADAGKGVLLAGHDPDIM
                     AWCCHRIIFLEHGRVTLDAPAADAMAELARRGERDYVCSV"
     gene            14184..14903
                     /locus_tag="PLM74_00530"
     CDS             14184..14903
                     /locus_tag="PLM74_00530"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HON41602.1"
                     /translation="MSARSDTPIGLNPIARLAALVSTCVVAFRARTPLQAGVLCCAIL
                     LMHAATGAPLGQPSFRRRLRGIFLFSAILFASQILLARGPAPAERVAVGALMALRFLN
                     VVLASHLFVSTTEPECLAYALMQSGLPYRYGFAFLAAMRFVPYFRDQAQTVRQAQMAR
                     GIALDRATPKGVFLMARYTFIPVVVSALGRVESLSVSMEGRCFGLHPTRTFRKRIPFG
                     LVDALAIAASAALAILALAAM"
     gene            complement(14919..16130)
                     /locus_tag="PLM74_00535"
     CDS             complement(14919..16130)
                     /locus_tag="PLM74_00535"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HON41603.1"
                     /translation="MEVFVTRQPIFDTADKVYGYELLFRGGIENALASRGPDPVLLSS
                     ILDAEAITGGKRAFIRFPDDLIEHGAPAAMPGEFLGLLVPNDVKPSQELIQICRILKQ
                     KGYVLALDDFTQGSGQEALVELADIIRINFLHTSPAQLMWLSERPDLSRLELWALEVH
                     SAKAYREARELGCAYVQGCFFTQPVPSWGRMAASCSTAGLQLLNEVQKPEPDFGELAE
                     IIGHDPALSYRVLRLVNSAAFGLRTEVTSIKHALLILGMKEIKEWFPLLIMTTLTQDK
                     PDELVTVSVVRARFAELTARQTALVDRSAELFLAGLFSTIDALLDQPMHKAIAGMPFP
                     GDVQKALVDQDGPITPLYRLILAYEQGQWDEMSELAGQVGVPEQVLPRAYAAAVSWAR
                     EFCEQGGVTRE"
     gene            complement(16202..17044)
                     /locus_tag="PLM74_00540"
     CDS             complement(16202..17044)
                     /locus_tag="PLM74_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HON41604.1"
                     /translation="MPFSILDIIWLLVIIWSFVPLFTRQTRDASRYKLIRQTEHKRKS
                     RVITLIHRQESMNILGMTFARHIGIEDSEQLLRAIRITDPDVPIDIVLHTPGGLVLAT
                     EQIAHAIKSHRGKVTVFVPHYAMSGGTLIALAADEIVMDPNAVLGPVDPQIGSYPAAS
                     ILAAVAKKPVAEVDDQTLILADVAEKALRQVRSLVVEILKDRMPLEKAEQIAEALSTG
                     RWTHDYPITCEELTEMGLTACNDMPIEIYQLMELYPQPQAKRPSVGYIPIPYGARGEQ
                     GKRD"
     gene            complement(17152..18228)
                     /locus_tag="PLM74_00545"
     CDS             complement(17152..18228)
                     /locus_tag="PLM74_00545"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HON41605.1"
                     /translation="MHYTVQEGDTLWLVAQRYRSDYRMIALANSLEEPYEIRPGQVLH
                     IPRKYRLIGFISDYASTLGIADALRAPEPFSEVVYSFLHVQPDGDLHPGAQDPAVQLI
                     RARRTRVLAGVTNLTEARYSRQSADAALLAEDARSTLAGRIVEAASSNSLDGVHLDFQ
                     NISSTHWDSLAILAREVRARLALLSPNYTLSVSIPHDWAEGEFDLKHMAEFVDLMMLE
                     GLEKGAQDPEGPPTHLAWLQRNVESALEYVGRSKLAATIGVYGLDWDAHGEVAYITSD
                     AAARVASETGARIVRDADSAAAWFEYSDSRGEQHKVWYEDLVTFSAKIRALDSLGIRK
                     IALWRLGAVPAAVLEAASQAWVWG"
     gene            complement(18316..18849)
                     /locus_tag="PLM74_00550"
     CDS             complement(18316..18849)
                     /locus_tag="PLM74_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HON41606.1"
                     /translation="MSSVDVRRVTRTAILIALTVAFQSLGLQQMVTGPVVNMFLYLAV
                     MIVGIWGGVATGLITPAVALWVGILKPAMAPMVFPIMISNAALAVTFGLVSGIGGRKS
                     TTFRIIGILIASFVKFLILSTTVKYLVQVPGPLAKMMQGTQLATALIGGALALILIEI
                     LSRTGMFSETTGGTGRS"
     gene            complement(18890..>19294)
                     /locus_tag="PLM74_00555"
     CDS             complement(18890..>19294)
                     /locus_tag="PLM74_00555"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HON41607.1"
                     /translation="IAMHNLPEGLAIGASYAHGSSLGFSVALTIALHNIPEGMAMAAP
                     MCAARLDALQITKWGALAGLPMAIGALVGVMVGNVSSAVLSVCLGFAGGAMVFITADE
                     LIPDAQEFAVGHSGTFGIVLGALAGMILVYSL"
BASE COUNT         3645 a         5380 c         6337 g         3932 t
ORIGIN      
        1 ggatatttgc atgtgatgta gaaacttgac gttgtggggt gacctcatga agacactaat
       61 tctggtggat ctgcagaacg acttcatgcc aggcggggct ctggctgttc caggcgggga
      121 cgaggtattg cccatcgcca atgccgttca aggctgcttc ggcattgttg ttgcgaccgc
      181 tgactggcac cccccgggtc atggaagctt cgcgtcggcg catcctgggg cgcgagcagg
      241 ccagcttgtt gagctagcgg gcatgccgca ggtgctttgg cccgatcatt gtgtgcaggg
      301 cacgggcgga gctgagtttg cgccggggct tgatactgct cgcatagcta agatcatcca
      361 caagggaatg gatcccagca tagacagcta cagcggattc ttcgacaatg ggcatcgacg
      421 ctcgactgga cttgaaggct atttacgcag tcggggggtt gatgccattt acgtcatggg
      481 gctagctact gactactgcg ttaagttcac ggcgcttgac ggcgtggcac tgggtttttc
      541 aacgtatctg atagaagatg gatgtcgagg tgtggaagtt gcgccgggcg acgttacccg
      601 cgctgtgtct gagatgcgca cggcgggagt gcggataatc aacagccgac agctgggtgt
      661 gtccggggga gcgccgaggg aatgacgctt tctccgatat ccataaatac gtatgtagca
      721 gggagtgtct gcacgtgaga ttcaaaggtc ttgttatcgg ggtccccaag gagatcatga
      781 agggcgagcg tagggtatct gctgttcccg acactgtcaa gaagatggta gcggagggtg
      841 cgaaggtcct ggtggagaag ggagccggag agggctccta tttccatgat catcagtatg
      901 cagaggccgg cgccgagatt atcgagagcg ctgcagacgt gttcgccaag gcgaacatca
      961 ttctcaaggt caaggagcct atcttcaacg aggccatcgg caagcacgag gtcgatctga
     1021 tgcatgcagg gcagtgcata atcaccttcc tgcatcctgc gtatccgggt aaccacaaga
     1081 tgataaagga catgtcggcc aaaggaatct tggcgcttac gcttgacggc atcccgcgaa
     1141 tatccagagc acaggctatg gacgctctga cctccatgag tacggtggca ggctacaagg
     1201 cggtgctgat ggccgccaat aggctgccca aattcatgcc catggtgggc accgcagtgg
     1261 gtatgatcaa gccgtccaac gttctggtca taggcaccgg cgttgccggt ttgcaggcgg
     1321 ttgccacggc gaagcggctg ggcgcggtgg tctatgcggc cgatatcagg ccggctgcga
     1381 gcgagcaggc gaagagcgtg ggagcgaagg tcgtcgacct tggcgttccg gcggacctgg
     1441 ccatcggcga gggcggatac gcgctcaagc tgcctgaaga gtggctgctc aaggagcgcg
     1501 aggtgttggc cccgatcgtc gcagacgcgg atatcgtggt cagcgcggct ctgatcccgg
     1561 gcaagttggc tccgattctg atcactgagg agatggtcaa atcgatgcgg cctggttcgg
     1621 ccattgtcga catctccatc gaccagggcg gaaactgcga attgagcgag tccggtgtga
     1681 tacttgagaa gtacggcgtc agcattgacg gcaccaagaa tatccctgga atggtgccta
     1741 ctgcgtctac ctggatgttc gcgcagaata tcttcaacta cgtggccaac ttggtcaaag
     1801 acggcaagat cgacgtcgac atgtccgacg agatcattgc atcttcgctg gtgtcccgcg
     1861 acggtgttct agtgcatgcc ggagcgctcg aggcaatgaa cgccgtggac ggcggcaaca
     1921 agaagtgcgg gtgcaattgc gggagcaagt aatagggcga accagtaggg agttgtgagt
     1981 atagtgtctc cgctttctct cgtaattctg tttgtcatcg cgacgattgt ggggtacaag
     2041 gtgatcagcg ccgtccccag tctgttgcat actccgctga tgtcgggcac gaacgctctc
     2101 tcgggcgtta ccgtgctcgg ggccctagcc gttactgcta tggctcagac gatgggcaat
     2161 tctgcgatgg ggcaacttct cggcgcggtg gccatcgttt tggcgatgat caacgttgtc
     2221 ggagggttcc tcgttaccga cagaatgctc aggatgttca aaaagtagct agatagggag
     2281 ggttcagcgg agcaacccgc gctgagcggc gtcgtccgct gtatgatgta atgagctgga
     2341 aggaagcgat ctttcttggg ctcggcctcg cagacatact ggggttgatc tgcgtgatcg
     2401 gcacggtatg gggcatcaag atgatgagct cgcccaccac agcggtgaac ggcaatcttc
     2461 tgggcgccgc cagcatggcc ggagccatca tcgtgaccct cgtatccgaa ggcatagtcg
     2521 gaataggcat cctgtgggtc gctatggcag tgggcacggt gctcggatat gtcatggcga
     2581 ttaaggtagc catgatagag atgcctcaga tggtggcgtt gctgaacggg ttcggaggcg
     2641 gcagttcagc gatagtctcc ttgatgacct tggttgcgac ggtgtggacg gccggtacgg
     2701 tcacgttcgg agcattcgag aaggtcacgg ctgtgctggg cctgattgtg ggaggcgtca
     2761 ccttctccgg gagcctcgta gccgccggca aactagccgc gaagatcaac cagaggccaa
     2821 tcatctttga acggcaatcg gcaatcaata acctggctct cattgtcacc atggtcctgg
     2881 gcgtctcggc gattgtgtcc gacgggaccg gcatggttgt ctacgcagta ctggtgttga
     2941 tcggatcgct ggcgtacggc gtactgttca cgatcaaggt cggcggcgcc gacatgccga
     3001 taacagtctc tcttctcaac tcgttttcgg gcgtggcggc gtcgatttcg ggattcgcca
     3061 tcggcaatcc ccttctcatc gccatcggcg ccgtggtagg cgcttcgggg ctgattctga
     3121 cgcagataat gtgtcgggcg atgaaccgca ctctgggtca ggtgcttgcc ggcaagacga
     3181 cggtgctcca cccgacacac ggccagaagg cagcggctgg ttctgccggg ccttcccggc
     3241 ctgttcagcc tagcggcgct cccgcacagg ccccgcctac ggctgcggaa ccggcgcccg
     3301 cgggagagcc gaactacatc gggatcgcct ggtccgcgaa gagcgtgatc gtagttccgg
     3361 ggtacggcat ggccctggcg caggcgcaag gcgacgtgaa ggcgcttatg gaccggctgg
     3421 aggcgggcgg agcagaggtc aaggtggcca tccaccctgt agcaggacgg atgcctgggc
     3481 atatgaacgt gcttctggcc gaggtggatg tgccgtacga caagctctac gagatggacc
     3541 aggtcaacga cgagttcgcc aagactgatc tagtgatagt ggtaggagcg aacgatgtga
     3601 tcaaccccgc ggccaacacg gctgagggga cacctatcta cgggatgcct atcctcaacg
     3661 tgcacgaggc aaagcacgtt atcatattca actacgatac caagccgggt tatgccggcg
     3721 tcgacaaccc gctctacgag ccgagcgaca aggtcacctt gctgcttggg gatgcggcga
     3781 cgacagtgcg gaagttcatg ggcgatatcg aagcgactgc gactgcgacg gcatctgccg
     3841 gtggctcggc ggaacccgca gagtcaaagg ccgagctcgt gcagatcacc agggaagcca
     3901 ggagcgttat cgttgttcct ggctatggca tggctctagc gcaggcgcag ggcgacgtga
     3961 aggcgcttat ggatcggctg gaggcgggtg gagcggaggt caaggtagcc atccaccctg
     4021 tagcaggacg gatgcctggg cacatgaatg tgcttttggc cgaggtggat gtgccgtacg
     4081 acaagctcta cgagatggac caggtcaacg acgagtttgc ccagactgat ctggcggtag
     4141 tcgtaggagc gaacgacgtg atcaatcctg cggccaacac ggctgagggt acgcccatat
     4201 acgggatgcc tatccttaac gtgcacgagg cgaagcatgt aatcatattc aactacgata
     4261 ccaagccggg ctatgccggc gtcgacaacc cgttgtacga gccgagtgat aaggttacct
     4321 tgatccttgg cgacgcagcc aagacagtac gggagttcat gaacgaagtg gggttgtagc
     4381 gctcggtcat gcggcccaac gcggcatggc gtactatgat gtcatgacat gaaataggcc
     4441 cttcgcccac gatcccatct agcctggatc gtgtcgggcg aggggccttt ctttctatgc
     4501 gtactgcact gtgcacaatc gcttaggact agctgcttct gacgagccgc gccaggctgc
     4561 agcctggcgc ggctactcgg cgggtgtacg gaacggccca ttgagcagag cagacagcgc
     4621 ttcgtctgag acggccagcg catcgactga caccccgtac gcgcctgctg ctctggccgc
     4681 gtccaccagt cctgcaagtg cgggcgcaga gcttttcgcc tttggtatgg acgctgcgtc
     4741 tatgccggct acaaccggga ttttggtccc tgcgaaaccc tgcgcggcgc ggaccagagt
     4801 atccacgagg tctgcaactc tggtatttgt gagaggaacc acgaaatcca gccagcccgc
     4861 ggcagcccac ttgctccaat cctgcattct gaccagcacc gagtcagaat atggatacac
     4921 agacgcggag acgagcgtgc ccggagattc tgcgcggatt gtggacacca gccttccgac
     4981 aaaggcgctc agattgctgc atctccagtc aagccaggca ttccacctgg cgatattgag
     5041 cccgatggag cgcggcgcga agcccaggtc ggcttgggcc aactgcaata cggtggggtt
     5101 gaaatcgtac ggcgcggcag gcccggcagg gtagcatatg gatccgagat ggatgccgtt
     5161 cggggcatag caggtgacca actcgcgcgc tatgtccatg atgaactgct gaacttcggg
     5221 atgggccggc gataggaagt agtgcccgtc gggggccgcg gtggggcccc ggtcgtctcg
     5281 cttcatagcc gcccacccac ggtttgcctg aagcagccga ggtagagagc cgtcggtcgc
     5341 ggcttggccg acatcgaagg cttccaccca cgcgtgcact gagaacccca ggagttttcc
     5401 ttcttggatg caagcctgaa gtggatcgaa gtcgccatag acgggattgc gctcagctac
     5461 ctgcgacggg tagagggctt gaccttgcga aaaggccgac acgaacagtg tgttgaaacc
     5521 ggccgccgcg agccgcttca gatcgtgggt tatgcgttcg cggtcagtgg gcggagtgta
     5581 ccacgcggct ttagtcttga gctggcccag gttgaactgg gcgccgtatt ttacgttcac
     5641 aaggtatgct atggtgtttg taatgagcgt ggctacgtcg ggcgtagtgg cattcgccgc
     5701 gctaaacagg ttctcggagc agtagacccc atatcttgtc tcggtcattg cagggcgccc
     5761 atccgaccat ttggccagca ccagggcgtc gtggttcacg cggtttacga gccctcgctg
     5821 gcgcgggacg ggtatcgtag aacccagccc gcggaagatc gggtgagatc cggccgatgc
     5881 gatcaaggga tctgcactga ctttctcagt cgatgagtat cttgccccca tgatctcggt
     5941 gagctcgtag tcgggatgaa cacgccagaa gtcgtcgcgt agggaagtgt cgaaggttgc
     6001 aaagacgcgc cccccggctt cggcgaattt cctgatgttt tcggccgcca gctgcgatat
     6061 gcatgaagtg gacggcagca ccactgcctt gtaagaacgg agagctccta tctccaagtc
     6121 gtggtcggtg atcaccgcgg ccgatatccc tgccctcgcc agggtgctga cgagccagtt
     6181 gacgtcgacg aactccgacc ggtagtccca tgcgttgagc gacgcgtaat ggcgtgtata
     6241 acccgagtcg actatcgccg cgtccgctcc ctggcgtgcg tacggcgact gttgctccgc
     6301 agtcgccagc gccgccggac ccagccaaat cacggctgca attgcggcgg ccacgatgaa
     6361 agccgcgcga accacgcgag gcccgcaggt catgactgtt ttgccgttcg tgcgaatcaa
     6421 cacttgctca tcctccttgt tcggtttcat tcttcgtttc gtgaggtttt tcctccaacc
     6481 gggctgggcg ggcgaaggaa tagagcctcc ggaaaagaac ataccaagaa acgagtcgag
     6541 cagcgtttga gcgaggatgg cgatctatgg agtactacga ttacacacaa cccttcgcgt
     6601 cgaggcgcag cccggtgatg ggaatgcggg gagcggtggc cacgagccat ccgctggctg
     6661 cgcaggcggg ccttaggatg ctgcttgccg gcggcaacgc cgtcgacgcg gcgatagcga
     6721 ctgcagcggc gctggtagtc ttggagccta cctcctgcgg tttgggcgga gatgcgttcg
     6781 ccatagtatg ggacaacggc gagatgcacg ggcttaatgc ctcggggcgt gcgcctcagg
     6841 cgctgagcgc tgaggagctc cgacgcgccg gtttcgccaa tatgcccgcg gaaggctggg
     6901 cgcctgtgac ggtgccgggc gtcgtgtcgg catggtcggc cctgtcgcgc agattcgggt
     6961 gcctgccgtt ccgcgacgtg atggcgcctg ccgtggagta cgcccgcgag ggcgcgcctg
     7021 ttcctccggt gatcgccggt tactggagcg cggcccagaa acggttcgga gggcgcggcg
     7081 gggttttcgc ggagtttgag cacgcgtttc ttctgggagg tcgggcgccg cgtccgggag
     7141 aggtgtaccg caattcggag cttgcgatga cgctccagtc tattgccgat tcgcatggag
     7201 agtcgttcta ccgggggggc ctggctcgcg ctatcgctgc gcattcggag gcgaccggcg
     7261 gttatctggc cttgcgcgac ctcgcagagc atcttcctga atgggtcgaa ccggtgaatg
     7321 ctcgttaccg cggattcgat gtgtgggaga tcccacccaa tgggcagggg attgtggcgc
     7381 ttatggcgct gaacatcatg gaagggttcg atctgggcga catgccccgc cttgcgccgg
     7441 aattgctcca cgtgatcatt gaggccctga ggcttgcatt tgccgatgcc catgcctacg
     7501 ttggcgatcc aagagctgtg agcgtgcctg tgtgtgaact tctatcgccg gagtatgctg
     7561 cggcaagaag agcgcagatc agcatgtctc aggccatggc cgacgtcgct ccaggtcggc
     7621 tgagcgggaa actggagacg tgggaaccgc atgacggcga caccgtatac ctatccacgg
     7681 cggactggga aggcatgatg gtgtcataca tacaatcaaa ctacatggga ttcgggtcgg
     7741 gtgtggttgt gcccggtaca gggatatcca tgcagaacag gggcgccggg ttctcgcttg
     7801 aggaaggtca ccctaacgag ctggctcctg ggcgaaggcc gtaccacacc atcataccgg
     7861 ggttcctcac tcagtcgggt gttccgctgg ccgctttcgg ggtgatgggc ggcgatatgc
     7921 agccacaggg gcatgtgcag gtgatctcga gcatcattga tcattcgctc aacccccagg
     7981 ctgccatcga ctgcccgagg gtgagagtca tgggcgccga cggcgtgctg gccgagccgg
     8041 aggttggggc ggataccata gctgccctga ggcgactggg gcacaacata cgcgctgaag
     8101 agggtcgcgc cggattcggc ggcgggcaga tggtgtggcg cgatccggat acgggtgtgc
     8161 ttattgcagg cagcgagccc agaaaggacg gctgcgctgt tgccatatga agtccgcgga
     8221 tatgcatgca cacgtgcatg agggaggtgt caagagcatg cgaggatcaa ttggcgcttc
     8281 aatggtcttc ggcatagtcc tggtactgtt gggagcgatg ttcctcctga ataacctggg
     8341 cattacgacc atcagcattg gtcgcattat cgagacatac tggcctatcg tcctggtggc
     8401 cctggggctt tcggcggtgc ttaagccccg tgacgacggg cgggtacgtg atgagcggac
     8461 atatgatgac cagatacacg ataggccgac ggccttgtac gatgaggccg gctcgggaaa
     8521 cgaagaggct gagccggccg ggccgcgcga gataggcgaa gcacccgagc cgtgtcgcgc
     8581 ttcggggccg aatgtgggag gatggatcct cattggcctc ggggtgctga tgctcgcggc
     8641 gaacacgcgg ttctgggggt tcgatctgag ccgactgtgg gggacgttct gggcgctcgt
     8701 ggtgatcctt gtcggctgga cggtcctgcg gtcgtcgccg cccgtcggcc aggtggggcg
     8761 cactcagtgg gtggtcatga gcgggctgga gcaacgcgcc gccggctggc cgctgcgaag
     8821 cggatcgtat gtcgtcgtca tgggcggcgc cgacctggat ctgcgtgtgg ccgatgtcct
     8881 cgatggcgaa acgtatctgg acatgactgc gattatgggc gcgatagagg tgattgtccc
     8941 gccggacata agtgtggagt gtgagggggc tgccattcta ggcggggtga cggtgctcgg
     9001 tcagtcgtcg ggaggggttc tggccagccg gtcattcgtc cgggaagcgc ctgcgggctc
     9061 cacgaagcgg ctacgggtca ggtgcagggc gatcttgggc ggagtagagt tcaagtagag
     9121 tcccggtgga gtcccggtgg agtcccggtg gagtcccgga gcaacttagg tagggattct
     9181 gcctttagtg tcgaagtatt acaaagtcac tgattacact cctccgagtc ccctcgccta
     9241 atgttcgggt ggcggcgccg ctgcccgagg ctatggtagg cggaccgaca gggtgcgccg
     9301 ggaaacgggc gcgcctccca gtgcggaaag gaggcggaat ggtgtttcgt atctgtacgc
     9361 cgcaggcccg aggggtgcat aggcgacgcg ttttgcgccg cagtgccctg gcgcttgcgg
     9421 cctttgcttt cgtattcgct gtgacgactg tcttgcctgt cggtcgggta gaggcgctct
     9481 tcggccgctc agggctgtca tttgcggtgg tgggcgatag ccaggggcga aacgaagtat
     9541 gggacagggt gatcgacgcg gtcaatgctt cgcgtccgca gttcgttctg cactgtggcg
     9601 acatggtagc atcggggaca agccagcagt acgacgactt tgtcggaaga gcccgaaaac
     9661 tccggatgcc ctggtatgcg gtgctcggca accatgacgt gaagggcgac gggaaagcgg
     9721 tgtacgccag gctagtccgt aaggacgcct actactcgtt tgtgcagaag ggctacaggt
     9781 tcataggcct cgacagctcc tcaggtagtc tcgatgcgac tcagattgat tggctccggc
     9841 gggaactgca agcgccggag cccaaattcg tcttcatgca catccctctt ttcgatccca
     9901 tgcccggcgg cgaccattgc tttctcgaca ccgggcaagc ggccgagctc cataagatgt
     9961 tcagcacttc tagggtattg gctgtgttca gtgggcatgt gcatgtcttt gctcaagcgg
    10021 cgaaagatgg cgtgacgtat gtcacgaccg gcggcggggg cgctctgctg tatgcccggg
    10081 ctgagcaagg cggatttcac catttcacca tggtgcgggc agatggcgac cgcatccagg
    10141 tcgaggctgt gcccgtgaag gctgagatcg aagaaccgcg cctcacagtt cggggttcga
    10201 aagggcaggt ggagatgacc ctgggcgaga ttgcggccat gccccggttt gaggccaaaa
    10261 cggctttcga gaacaagttc gccaacctgg gcgggcaggg ggtgtatgcg ggagtccgcg
    10321 tagccgcgct cgtcgacatg gtaggcggaa tgcagccagg gcagatcctt gtagtcacgt
    10381 cgagtgacgg ttattcacaa gagtatgcct atggaaacgt gtatccgtct gtcgcgtggc
    10441 tgtcgctcca gggcgagatg ctcttggcct atgagaagga cggttcgact caccccgcct
    10501 ggggagaatg cccgcgaatc gtgttcgctc cgtccgacgg cgtataccac aactcggact
    10561 gcgccgcgac atccgctccg gggcagggct ggaatgtata tgaatcggcc ggtgcgcgtt
    10621 gggcgcgcta tgtgaggcgc attgaggtca ggacaaagta atcgggaggt agtcacgaca
    10681 tgaagaagaa caacgctctc agggtgctgg caatcgtagc atttgcctgc ctggttcttg
    10741 gcatggggcg ccttgccgcg gcgggcgaat ggcaagtgga agtggtgtct ggcgacaagt
    10801 ctgtggtggt taccgcagct gatgcagcgc cacaggcaca gacggtgaaa gccgggtttc
    10861 tcaggagtac cgggcgcata gagggcccgt cggcctatgc cgtcatgcct atggacgcga
    10921 ttctcgcccg cgtgggcggc atccgcccgg agcaggccgt ccgagtgacc gcacgggacg
    10981 gctatcagat ggtgtacagc ttcgcgcagg tccagggcgg catctttacg tacgatgata
    11041 agggcaatgt gttgcgcgtg ggtggcccat cgatgcttct tgcgtttgag tcggatcgcg
    11101 actcagccga caagttgccc cggattatat acgggcgacc cgacgacatt gtcataaccg
    11161 atggccactt ctgggccaaa tcagttgcca aggtagaagt cattccgggc gtggaggact
    11221 ggcagatcag cttgtcggga gtggagaaga cgtggctgga cagagccacg ttcgagagca
    11281 tagtgacatg cccggtcact cctcaccccg cggtcacctg gtcggtagct gagaaggacg
    11341 ggagcacgtc tacttatgaa ggcgcgcccc tttgggtcat tctttcgata atcgatggag
    11401 gcgacgcgcc cggagggcac tacatgttca acgatgacct ggcggagaag ggctacacca
    11461 tcaaggtgat gtcgaaggac ggtctcgcag ctgagttcga ttcgaagtcg gtcgcacgga
    11521 acaacgatat catcgtggcg tacttgaaga acggcgagta tctcacagag ggcgacgcgc
    11581 cacttcgcct ggtcggcgat gtgcctacca agaagcattc ggtcaaggcg atcgcggcga
    11641 tcgagatcgt cggcaattag tatgtcatcg cggttggcca agcgccggcg gggctactac
    11701 ttcagcacgc gcgacctggt cctgatcgcg gcggtgagcg cagcaggcgg tgtgctaagc
    11761 acgtacgttg gatacctagg caacctgctg aactcggcgt tcggcgtgcc gtttggcgcc
    11821 ggtcagttca tggcggggtt gcacgtgttc tggttcgtgc tcgccagggc gattgtgggc
    11881 cgtagcggcg ccggcgccct ggccggagtg ctcaaaggcc tggtagagtt gttcacaggc
    11941 agcactcacg ggctgccgat agtagttgtg tcggctgtag aaggcgtgct tgtcgaccta
    12001 ctgtcgctgc cgttcgtcag gccaccgcta ggcgctatgt gccttgccgg ggcagtatcg
    12061 tcagcatcca acgtccttgt gttccaggcc atgtacttct ccggagtctc atggggatac
    12121 gtgggcttga tggtgctgtt tgcggcgata tcaggggcgg tgttcggcgg atacttcacg
    12181 gcgggtgtgc tggatgtggt ggagaccgcc ggtctgctgc gaccagtgga gatgtccacc
    12241 cccggttcgg cgccggggcg caggccacgg cctgggcggc gtgttgctcg tctggctatg
    12301 actcttgtga tgatagctgc gctggcaggc ggggcggtat actactacac ccaggtgttt
    12361 gagcccgtcc caagcagccc caggtgcaag atcgagggca aggttgcacg ggcctacgag
    12421 tatgcgccgt ccgggtttcg agatgaggtc gtgtctgtcc gagcggagct ggtggggcag
    12481 gtcacctatg tgcctgaagc cgattactcc ggcgtgccgt tggcggtgat cttggggcgc
    12541 gccggagttg acccatctgc ggcatcggta cggatcatcg cttccgatgg atacgaggcc
    12601 atcttcagcg tgcgagacat cacggagcat acaaacgtgc ttctttgcca ggacggcgag
    12661 actttgcgaa ttgttgcccc cggctttgag ggcgcgttct gggtgaggat ggtgtccagg
    12721 atccgcgttg agtagagaaa tggtagagct ttcgaggttt ggtgtggatt atccacaagg
    12781 cacttcaccc gcgcttgcgc aggtggactt gtcgatcggc cccggcgaat cggtgctcgt
    12841 gacagggccc agcggttcgg gcaagtccac actagcccta tgcttgacgg gcgttatacc
    12901 caatgcggta tacgcgcgcc tctccggagg catacgggtg tgtggagtcg aacctgctgc
    12961 gcaaggcgtg tacgacatgg ccagccgcgt cgggctagtt caacaggacg ccgaggcgca
    13021 gttctgcacc atgagtgtgg aggacgaggt ggcgttcggg ccggagaacc tgatgtgttc
    13081 ggaggcagaa attgagcgcc gggtagcaca ctcgctggag ctggcgggcg ccgaacatct
    13141 ggcggggcgt gccctaagtg agctatcggg cggcgaaaag cagcgcgtgg ctatcgcatc
    13201 cgtcctggcc atgcaaccac aactgctcgt gctggatgag ccggccgcgc atcttgaccc
    13261 gcgtgcaaca agggcggtag cggattcgct gcacgtagta cagcgcgact tggggctctc
    13321 tgtcattatc atggagcata agccgtggag attcgccggc tcaactcggc gcatggtaca
    13381 tctggacgag ggacgagtcg tacatgacgg gcccgtcaag tgggacaggc ccgggcatct
    13441 gcactctccg gttgcgcgca gctcacaacc acgaccaggc ggaccgctgc ttgccgtgcg
    13501 cgggctgagc catgcgtacg gcaagcgaac tgctctcgcc gacgtgcacc tggacgttgc
    13561 acgcggcgaa atagtgggga taatgggcga caacggctca ggcaagacat cgcttcttct
    13621 cagtatcatg ggcatagtca gacctcaggc aggtcgtgtc acgcttcgcg gtgaggatat
    13681 caccgcgttg ccggtgtcag ccagagcttc ccgcatgggt ttcgtgttcc agaaccccaa
    13741 tcaccagctg ttcaccgact ccgtatggcg agaggtggcg gcagggccgc tggcacgggg
    13801 tgagtctgcc gaagagtgcc gacctgcaac catgagcctt cttcaacggt tcggcttggc
    13861 cgatatgtgc gatcgccacc cgctgacgct cagtttcggc cagaagcgca ggcttaacct
    13921 ggctgccgcc ttgatttgcg atcccgacct cttgatgctg gatgagcctt tcgcggggca
    13981 ggacatgagg cgagcggagg acctcgcctg cctactggct gaagtggccg acgcgggcaa
    14041 gggggtactg ctggctggcc acgatcccga cataatggcc tggtgctgcc atcggattat
    14101 ctttctggag catgggcgtg tcacgctgga tgctccggcc gccgacgcca tggcggaact
    14161 tgcgcgcagg ggggaacggg actatgtctg ctcggtctga cacaccgatt gggcttaacc
    14221 ctattgcgcg tctggcggcg ctagtctcca cgtgcgtcgt ggcctttcgg gcgagaacgc
    14281 ccttgcaggc gggtgtgctg tgctgcgcga tattgctgat gcacgcagcg accggcgcgc
    14341 cgttgggcca gccctcgttc cgccggcgtc tgcgcgggat attcctgttc tcagcgatct
    14401 tgttcgcaag tcaaatactg ttggcacgcg gccctgcgcc ggctgaacgt gttgctgtgg
    14461 gcgccctcat ggcgctgcgc tttctcaacg tggtgctggc gagccatctc ttcgtgagca
    14521 caacagaacc cgagtgcctg gcatacgcgc tgatgcagtc gggactgccc tacaggtacg
    14581 ggttcgcttt tcttgcggcg atgaggttcg ttccgtactt tcgcgaccag gcccaaacag
    14641 tgcggcaggc ccagatggcc aggggcatcg cgctggatcg cgccacgccc aagggcgtgt
    14701 ttctgatggc ccgatacacg ttcatcccag ttgtggtgtc cgctctgggc agggtcgagt
    14761 cgctctctgt atcaatggaa gggcgctgtt tcgggctcca tcccaccagg accttccgca
    14821 agcggatccc gtttggtctg gttgacgcac tggcgattgc ggccagcgca gcactggcca
    14881 tcctggcgtt ggccgcaatg tgacctatcg tctgccgcct actcgcgtgt gactcctccc
    14941 tgttcacaga actcgcgggc ccatgatacg gcggcggcgt acgcacgggg cagcacctgt
    15001 tcaggcactc ccacttgccc tgctagctcg ctcatctcgt cccactgccc ctgctcgtac
    15061 gcgagtatca ggcggtacaa gggggtaatc gggccgtctt ggtcgaccag agctttctga
    15121 acgtctccgg gaaaaggcat gcccgcgatg gctttgtgca tgggctggtc cagcagagcg
    15181 tcgatggttg agaagagtcc cgctaagaac agctcggccg atctgtcgac tagcgcggtc
    15241 tgcctggctg taagctccgc gaacctggct ctcaccactg acacggtgac cagttcgtcc
    15301 ggcttgtcct gagtgagcgt ggtcatgatc agcaatggga accactcttt gatctctttc
    15361 atccccagaa tgagcagagc gtgctttatc gaagtgactt cggttcgaag gccgaatgcc
    15421 gcggagttga ccagcctgag cactcggtac gagagtgcgg ggtcatgccc gatgatctcg
    15481 gctagctctc cgaaatcggg ttcgggcttc tgcacttcgt tcagaagctg aagaccggct
    15541 gtgctgcaac tggcagccat gcgaccccac gacggaacgg gctgagtgaa gaaacaccct
    15601 tgtacgtacg cgcaacccag ctctcgggct tcgcgatagg ccttggcgga gtggacttcc
    15661 agagcccaga gctcgagccg gctgaggtca gggcgttctg agagccacat aagctgggct
    15721 ggggatgtgt gcaggaagtt gatcctgatg atgtcggcca gctccacaag ggcttcctgc
    15781 cctgaccctt gagtgaaatc gtcgagcgcc agcacgtacc ccttctgctt gaggattcta
    15841 catatttgga taagttcctg agaaggcttt acgtcgttgg gcaccagcag ccccaggaac
    15901 tcgccgggca tcgcggcagg tgcaccatgc tcaatcaaat cgtcggggaa ccggatgaac
    15961 gcgcgtttac cgccggtgat agcctcggca tcgagtatgg acgatagcag cacagggtcg
    16021 gggccccgtg atgcaagcgc gttctcgatg ccgcctcgaa acagcagctc atatccgtat
    16081 accttatccg cggtatcgaa gatgggttgc ctggtaacaa agacctccat gtcaatcctc
    16141 ctgtataatc gctgagccgc cgccctcacg ctccatgatc ggacgtctgc ccgctcccgc
    16201 ctcagtctcg tttgccctgc tcgccccggg cgccgtaggg gatgggtatg tagcccactg
    16261 aaggcctctt ggcttggggc tgggggtaga gctccatgag ctggtatatc tcaatgggca
    16321 tgtcattgca cgcggtgagg cccatttcgg tcagctcttc gcaggtaatc gggtaatcgt
    16381 gagtccagcg accggtagag agagcttccg caatctgttc ggctttttcc aggggcattc
    16441 tgtctttcaa gatctctaca actagcgagc gaacctgcct gagcgccttc tcggcgacat
    16501 cggccagtat gagggtttgg tcatctacct ctgcgacggg cttcttggct actgcggcaa
    16561 ggatcgaggc cgcgggatag ctgcctatct gcgggtctac cgggcccaag acggcgttgg
    16621 gatccatcac gatctcgtct gccgccaagg ctatcagagt gccgcccgac attgcatagt
    16681 gcggcacgaa cactgtgacc ttgcctcgat gactcttgat ggcgtgggcg atctgttccg
    16741 tggccagtac gaggccgccc ggggtatgca gaacgatatc gatgggcacg tcggggtcgg
    16801 tgattctgat cgctcgcaga agttgttcgg agtcttcgat tccgatgtgt cgcgcgaatg
    16861 tcatgcccag gatgttcatt gattcctgac gatgaatgag cgtaatgacc cgggacttgc
    16921 gcttgtgttc tgtctgtcgg atcagcttgt accttgacgc atcacgggtc tgcctggtaa
    16981 aaagggggac aaagctccag atgataacga ggagccagat gatatcgaga atactgaacg
    17041 gcatgagccc acctccagtc gagcatgtcc actctcatta tagccacaga gtgccgcatt
    17101 ttccacaagc cggtgcaccg agcagccggc ctaacggcct gaggcgggcc gctaccccca
    17161 cacccaggcc tgggatgccg cttccaggac tgccgcgggc accgcaccca ggcgccatag
    17221 cgcgatcttt cgtattccga gcgaatccag cgctctgatc ttggcagaga acgtgacgag
    17281 gtcctcatac cataccttat gctgctctcc gcgggaatcg gaatactcga accaggcagc
    17341 ggcgctgtcg gcatcacgga caatgcgggc gcccgtctcg ctggcgaccc gcgcggcagc
    17401 gtccgacgta atgtaggcga cctctccatg cgcgtcccag tccagcccgt ataccccgat
    17461 agtggccgca agcttggagc ggccgacgta ttccagggcc gactcgacgt tcctctgcag
    17521 ccatgccaga tgcgtgggtg gaccctcagg atcttgggcg cctttctcca gtccttccag
    17581 catcattaga tcgacgaatt cggccatgtg cttgagatcg aactcgcctt cggcccagtc
    17641 gtgtggaatg gagaccgata gtgtgtagtt ggggctgagc aaggccagtc ttgcccggac
    17701 ttcgcgggca agtatcgcaa gggaatccca gtgcgtgctg ctgatatttt ggaagtcaag
    17761 gtggacgccg tcaagggagt tggatgaggc ggcttccacg atcctgccgg ccagcgtgga
    17821 ccgggcatcc tccgcaagca gcgccgcatc ggccgattgc cgcgaatacc gggcctccgt
    17881 caggttcgtc acacctgcca acacacgcgt ccttcgcgct cggatcagct ggacggcagg
    17941 gtcctgggcg ccgggatgca agtcgccgtc aggctgtacg tgcaagaacg agtagaccac
    18001 ttcgctgaag ggctcgggcg ctctgagagc gtcggcgatg cccagagtgg aggcatagtc
    18061 ggagatgaac ccgattagcc tgtatttgcg agggatatgg agtacctggc ccggacggat
    18121 ctcatacggt tcctcaagcg agtttgccag ggcaatcatt ctgtagtcgg aacggtaccg
    18181 ctgggcaacc agccagagcg tgtcgccctc ctgtactgtg tagtgcatga accccgcctc
    18241 ccgcgaacgg tgatggccaa caggcatagc agtataagct atgccaaagg catcagaaat
    18301 gttcgcggat ccggatcagc ttcggcccgt gccgcccgtg gtttcgctga acatgcccgt
    18361 gcgcgacagg atctctatga ggatcagggc cagcgcgccg ccgatcagcg ccgtcgccag
    18421 ctgtgtcccc tgcatcatct tggccagggg cccaggaacc tggaccaggt acttcacggt
    18481 tgtcgacaag atgaggaact tgacgaacga agcaatgagg attccgatta tgcggaacgt
    18541 cgtggacttg cgacctccta tgccggacac caggccgaat gtgactgcca gggcagcgtt
    18601 ggagatcatg atggggaaaa ccatgggcgc cattgccggt ttgagtatgc ctacccaaag
    18661 cgccaccgcc ggcgtgatga gtccggtggc cacgccgccc cagatgccga ctatcatgac
    18721 cgcaagatac agaaacatgt tcaccaccgg gcccgtgacc atctgctgga ggcctaggga
    18781 ctgaaaggcc acggttaggg cgatgagtat cgccgttctg gtcactcggc ggacatccac
    18841 agaagacatg ccatctcttc ctttctagat atcatcatgc cgcagtgtgt cacaacgaat
    18901 aaacaaggat catgcctgcc agagcaccaa ggactattcc gaaggttccc gaatgcccaa
    18961 ctgcgaactc ctgagcgtcc gggatgagct catcggcggt gatgaagacc attgcgccgc
    19021 ctgcaaaccc gagacatacc gaaagcaccg cgctggacac gtttccgacc atgactccca
    19081 ccaaggcgcc tatcgccatg ggcaggccgg ccagggcccc ccacttcgta atctgcaggg
    19141 catccaaccg cgcagcgcac atcggcgccg ccatggccat tccctccggg atgttgtgca
    19201 gagctatggt aagagccacc gaaaacccga gcgacgatcc atgggcgtag cttgcgccta
    19261 tagccagccc ttcgggcagg ttgtgcatgg cgat
//
LOCUS       DAOTAC010000006        19038 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_2384_length_19038_cov_5.024970, whole genome shotgun sequence.
ACCESSION   DAOTAC010000006 DAOTAC010000000
VERSION     DAOTAC010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 19038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19038
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2384_length_19038_cov_5.024970"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="PLM74_00560"
     CDS             complement(<1..682)
                     /locus_tag="PLM74_00560"
                     /inference="COORDINATES: protein motif:HMM:NF013486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HON41608.1"
                     /translation="MSNCLGECARLEVEFKLGRVREFMREQELDALLLGRADNFAWIT
                     AGGDSRCASTTDMGVASVLITPGRQYILTSSIEIDRVRAETLAHIAPADVFEFVEYPW
                     HHGQDGMLGEIPGKLGADFSCTAAQGRFYDVTALRMPFTPWEADRYPGIGRGVSDAVE
                     RVCFRIEPGMTERMIAGMTAAELIQAGFTPTVVLVGADDRVRLRRHPTPTGNTLERYC
                     MIVVCAHQG"
     gene            complement(675..2003)
                     /gene="galK"
                     /locus_tag="PLM74_00565"
     CDS             complement(675..2003)
                     /gene="galK"
                     /locus_tag="PLM74_00565"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HON41609.1"
                     /translation="MALEHDRAVELFDRIFPGLRGSGADERARISIARAPGRVNLIGE
                     HTDYSGGFVLPVAIDRDIVIVFRPRDDGTVRLYSADYGEYSEFSIDGSSAIESDPLHP
                     WSNYFRGVAWALRARAEGSGGPPLRGLDAVIVGDVPRGAGLSSSAALEVASAFTLLSA
                     ADVGDDVLDPEQVAARRDIALACQKAENEFVGVRCGVMDQMASVIGRAGHAIFLDCSN
                     LHHELVPMFFEEMGVAMVVYDTGIRRGLGESRYNDRRGEAERGEALIAQALGCMDVCK
                     LSSVSSEQFEQVSHVLPASIARRCEHVVRENERVVKAVAALKDRDLHAFGQLMYESHE
                     SLRDLYEVSCPELDEAVEIARGTPGVFGARMTGAGFGGCTINLVSRDRVELLQSRLSE
                     IRRESPSGVPVAFAVEAADGARVCSVRRPSEKSDVLGVPGREGRLTANCE"
     gene            complement(1987..3105)
                     /gene="galT"
                     /locus_tag="PLM74_00570"
     CDS             complement(1987..3105)
                     /gene="galT"
                     /locus_tag="PLM74_00570"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HON41610.1"
                     /translation="MRWNPVLEEWVVTATHRQDRTYKPPEGFCPLCPTRNGECPTEVP
                     AADYEIVVFENKYPSFRCDPCTPAVPGTELQPTAPSQGVCEVVLYTDDHHATFADLSV
                     DRIANLVDVWADRYEELGALSYVRYVLIFENKGDCVGVTLHHPHGQIYAFPFIPPRAA
                     KELESSSKYATARGRCMFCDLLAEELADGRRVVAENEDFAAFIPFSARYPYEVHVMPK
                     QHRESITQLSRLERRHLARILSMVTRGYDSLFGFSFPYMMIIHQAPTDDGQYDYYHLH
                     FEFYPPHRTATKIKYLAGCESGAGTFINDTLPEQTAEELRAAIGRANEPTVARTGAQS
                     SAQSSGQSSARSSADDNAGINGTAETAGSGGGPGGFGA"
     gene            3429..4430
                     /gene="galE"
                     /locus_tag="PLM74_00575"
     CDS             3429..4430
                     /gene="galE"
                     /locus_tag="PLM74_00575"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HON41611.1"
                     /translation="MRVLALGGAGYVGSHVVRELCDRGHHVVVLDNLSTGHEQAVSVA
                     SHGAAELVVGDVGDRTLVSEVLRSREIDAVMHFAAASLVGESMGNPSKYWRNNVLCAV
                     ELLDAMVEAGVDRIVFSSSAAVYGEPRQTPIPEDHPKSPTSTYGMTKLAIEHMLAGYH
                     SAHGVKSISLRYFNAAGADASGDIGEDHDPETHLIPIVLKTALGLRQHLRIFGTDYPT
                     SDGTCVRDYVHVCDLAKAHLAAMERLAGQDGACAYNLGNGAGFSVREVLRCAEKVVGR
                     PIPAAEDQRRPGDPAVLVASSQLAQSELGWKPTQASLEHIIASAWAWHSAAPGGYGE"
     gene            complement(4467..5663)
                     /locus_tag="PLM74_00580"
     CDS             complement(4467..5663)
                     /locus_tag="PLM74_00580"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HON41612.1"
                     /translation="MIAIKNAVLFDGVREVGETSLLLDAAIVSVDRAVELPEDLECID
                     VDGAYVTPGLVDAHVYMGLWCTGAGSEGRDLSDSNGTVQPQLRALDSVDWDDANFGRA
                     LAGGVATVVTGPGESNIFGGQALAMRTEECPVDGRVVRGVASLRVALGHEPKTTYSKS
                     VQYPSTRMGVAAVVREQFMKAQEYAIGCREATDSGKRPKFDFTLQTLAEVMAQRTPVT
                     FYAAKANDIVTAVRITKEFDLSGIIVGATEGHMVAEFLGESGMPVVFIPMMDGRMSVE
                     TRNATFETAAALAEAGVDVAISTGAPSLPSEYLPVMAALCMKAGLARESALRAITGGP
                     AAMFGLAQNGLGNAVTAGPGHIACGAPADVVVWEGDPFSLTGRPKLVIAAGNVVFDAS
                     RDEAPW"
     gene            complement(5660..6808)
                     /locus_tag="PLM74_00585"
     CDS             complement(5660..6808)
                     /locus_tag="PLM74_00585"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HON41613.1"
                     /translation="MLLALTGGRVIPVANCDIDEGTVLIDSGRILDVGSRIEIPEDCE
                     TIDCAGRVVLPGFVDPHCHIGLFVEGEDTYWSDANEMSSPAVPQVRALDALDPFDIAF
                     QDAPAAGFTTVLVTPGSGDVICGQSTVTKTSGTVADQMVLRHPAGMKFAFGGNPKNPY
                     KTKAENHPTTRMGVAAVFREAMYRARDYTERKEADTSTPLDLGMEALAMVLQRKMPAR
                     VHVSRGDDILTVIRLCKEFGVDFTLEHVTDGHLVVGEIVDSGAPCVVGPFFRPRGVSE
                     TRNWSFVTPGVLAKAGVKVALTVDHPFTPLRHARYSAAMAIRAGMDERDALRAMTINA
                     AQIAGVGDRVGSIEPGKDADLVVMTDHPFKLQADVEMTLIGGKVVFRK"
     gene            complement(6822..7889)
                     /locus_tag="PLM74_00590"
     CDS             complement(6822..7889)
                     /locus_tag="PLM74_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HON41614.1"
                     /translation="MKQCELVCSNLETHGVDGLIVTSEPNVRYLSGFTGSESFLFLSH
                     TARVFITDSRYIEQAEDECAGFEVVRYRHPFPELETVLAGLAAKHGVCSLGFEKDSIM
                     FSLYYRLANSGLELVPTAGIVEALRYVKDDREIQLIAKAARIADEAFHHILEIVKTGI
                     EEKDIEIELDYFMKKAGAAAAGFSTIVASGPRSSLPHAVPSRRKIEYGDFVTMDFGAM
                     YGGYRSDMTRTIVMGRANDTQREIYETVKRAQAVGCSQIRAGVAGTDPDRAARDVIKG
                     AGFGDFFGHGVGHGVGLEIHEEPFMSPKCERTLAAGSVVTVEPGIYLPRWGGVRIEDT
                     VVVKEDGCEVLTLSPKELIAL"
     gene            complement(8092..8466)
                     /locus_tag="PLM74_00595"
     CDS             complement(8092..8466)
                     /locus_tag="PLM74_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41615.1"
                     /translation="MVKLVSYDWPGNIRELSNALEYAVNLATGTVIDTDCLPFQVLCP
                     PSAGAALGSGSVPGSRPDLGPDRYDREYPIGFAAKPGMTRSALEDALERYGTSTRAKE
                     AIAAAFGVSRATLYRRLREYGL"
     gene            complement(8476..9915)
                     /locus_tag="PLM74_00600"
     CDS             complement(8476..9915)
                     /locus_tag="PLM74_00600"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HON41616.1"
                     /translation="MTVLPEVQGFAQAVADAIAAVAGVEVAMVDENLLTVVGSGKYAR
                     HIGRTLERSSVSARALAEGRTMVIENPQYDDVCRQCTQKDKCTDIADIICPIMIEGRP
                     AGCVILVACTDSQKEILIGDMQRWINFLERMADLISRAFNERQQTARAEGLARQLDVV
                     IDSVNDGILTIGDSGVILHANQSAARLLKSTRDQLASCPVSDIFPQLSSDTLGEVSSS
                     EFETYSCVRGNREYWLTTVRPIADDFCPRGHVITFRGIEEIPRMVANYIRSERHSTFD
                     DILGSSAAMASVKSIAASIAEGDSTVLIQGESGTGKELLARAIYHESARRQGPFVAVN
                     CAAIPESILESELFGYEEGAFTGAKRGGKPGKFELANGGILFLDEIGDMPLHLQGKLL
                     RAVEERVVDRLGGRKAVPIDIRIISATNKDLRKMSMRGGFRPDLFYRLAVIPIVIPPL
                     RDHKEDIPEYVDHFVAKYAHDLRRNVTSV"
     gene            complement(10067..11029)
                     /locus_tag="PLM74_00605"
     CDS             complement(10067..11029)
                     /locus_tag="PLM74_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008374465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON41617.1"
                     /translation="MPGNEVLVEVKGLKKYFQAGRRRVVKAVDDVSFRIYRGETLGLV
                     GESGCGKTTCGKAILGLLSLTEGEVVFDGMKVHRLEPSERKEFTKRAQIVYQDPYSSL
                     NPRMTVKDIIGEGIDVHRLYRGKEQEERVCELLHTVGLNREHINRFPHEFSGGQRQRI
                     GIARALAVEPDFVVCDEPMSALDVSIQAQITKLLLRLQREENLTYLFISHNLAMVKYV
                     SDRVGVMYLGKLVELTTSEELYRNPLHPYTQALLSAIPVADPDEERSKERVVMKGEIP
                     SAINPPAGCRFASRCRFVTQVCREDEPALSEVGREHLVACHLAK"
     gene            complement(11019..12053)
                     /locus_tag="PLM74_00610"
     CDS             complement(11019..12053)
                     /locus_tag="PLM74_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON41618.1"
                     /translation="MRNGQMLNTKDLFVSFKTYAGIVQAVRGVSFELEKGETLALVGE
                     SGCGKTVTAKALMGLLPNEQVEIGGEVGFHGENLLEYGEDEMTKIRGKHISMIFQDPM
                     TALNPTMRIGDQIAESIIIHEQKTHAEARAEALEMLELVKIANAKERMNQYPFEMSGG
                     MRQRVMIALALACNPEILIADEPTTALDVTIQAEILELIMQLQRERDMGVILITHDMG
                     VVASISERIHVMYAGKIVEEGSVREVFYGPKHPYTFALMKSVPELSSTKQEPLYSIVG
                     TPPDLINPPQGCPFAPRCEFAMKICKLEPPAGVSCGETQRSYCWLHHPDADITGVPEV
                     LQGRSEAHAR"
     gene            complement(12062..12991)
                     /locus_tag="PLM74_00615"
     CDS             complement(12062..12991)
                     /locus_tag="PLM74_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012100848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HON41619.1"
                     /translation="MNVDGTVLAGDVFERVGVDEVSSEHLEKPALTYWADVWRRFRQN
                     KLAVFGLGLLVIIIVMLFLGPALSGKDYQYIDSSIRNQKPSAEHWFGTDDLGRDMFTR
                     VCYGGRVSILIGLLCTVVMIVIGFTLGALAGLKGGWVDNLIMRLVEFVGNLPYLIIVV
                     ILTLVMGRSLFALVFAMSLTSWVGTTRMVRGQILQLKEQDYVEAARALGSDTWRIIMR
                     HLLPNTLGIIMVQITMSVPGFIFGEAFLSYVGLGIRPPETSWGALASYGQEKLMFQPH
                     MLFFPSLMIVLTTLSFHLIGDGLSDALDPKLRR"
     gene            complement(13026..13961)
                     /locus_tag="PLM74_00620"
     CDS             complement(13026..13961)
                     /locus_tag="PLM74_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008374459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HON41620.1"
                     /translation="MARFLAKRVVYELVTLFLVITATFFLIAGAPGDPIAAKVEQMPE
                     RAQQVIRAKYGLDRPVLERYVRYMKNLILKGDFGQSIVYTGRRVNDVIRENAPISGRV
                     GAIALALDLVIGIILGLLMAFNHGRLADHVLRVCIVLAICTPLFVFAAILQYYVAFKW
                     RLAPIFGWGRPEHYIMPVLTMSIGGIAVYAKYMRSSALSVVGEDYIVTAKSKGCNRRR
                     ILQKHVLRNSMIPIVTMTGPSIAGIFGGSFIIEKVFSIPGLGRYYVKAVSDSDYTMVL
                     GLTVFFAVLYVASLIAVDIMYCVVDPRIRIPGTEG"
     gene            complement(14115..15809)
                     /locus_tag="PLM74_00625"
     CDS             complement(14115..15809)
                     /locus_tag="PLM74_00625"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HON41621.1"
                     /translation="MRKRTLILVAIALLCAGVFGGGATHASTASGLDKVQKFVFSSRS
                     DITGLNPLLHTTGPDNGVQSIILEGLVERVADEKGNAVIKLTSAKSWDLSKDGTVYTF
                     KIRDNAVWNDGVPVTANDFVYSFRMMADPSVGCTNAWLFDGVIQNFEEAHYDEGKKPQ
                     DIGVTAVDKKTVSFKLTKPCAYFPDLLAGVKPVRQDKYEQWGESYGSSIDKVVMNGPF
                     LIESWKPMVQMNFVKNPKHWDAANFTLQRIERKIIQDPATHVQALISGEIDIGATSDP
                     DWQEVLKKEGARFKTLKEPDNAPEFFGFNCSNKYFKNPKIRLAFSLAHDRERYIEDLL
                     YGRAEPLYSMMPSATMCGDKLYSERVGGKSQIVKTLMTKYPDPKALLIEGLKEEKLDP
                     NPAKMQIKYATRGTAEFSKKSAEWLLQNWQKTLGVTITIDMVEWNIMWDKVGKGDYDI
                     CTSGWGPYYNDPNALLEIFDPISGYFHSKRSGWTGPDADKYHQLLDKASFTVDPQARA
                     ELLLQAEQLLVGTGVIAPNYCGVATYYVANYVNGYYTNPHAGVDYTKLYTKGRPGR"
     gene            complement(16435..16944)
                     /locus_tag="PLM74_00630"
     CDS             complement(16435..16944)
                     /locus_tag="PLM74_00630"
                     /inference="COORDINATES: protein motif:HMM:NF018933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41622.1"
                     /translation="MESSQALLIHVTLKVQSYDCNAAMPELAQMLNAKLGATVKQGAA
                     KLYEKRGRWYLALSISVQVTPCVGTKVAGVDLGLANLAVVNCEGETLFFSGDHAAFVR
                     RRFKALRRRMGKAKALGAIRRMKDKEARSSVWRRRPSQPQGDSLWVYRLRPHRARGFR
                     GRRKHRESN"
     gene            16897..17406
                     /locus_tag="PLM74_00635"
     CDS             16897..17406
                     /locus_tag="PLM74_00635"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HON41623.1"
                     /translation="MDFESNVNQERLRGFHSGLKLHDIEPVSDLEISLATSEKENGCF
                     DYSAELDKSPVTAHRRFSEIVERLMSSDKPPTAVFCFDYHTTLLLIQSLMRMGLTPGM
                     QVSTIGFDAPRTACAPRVTSINQPALEMGAIAANLLLERISDPTLQPRTVRLSAQLEV
                     GDTTGPPTT"
     gene            complement(17572..18750)
                     /locus_tag="PLM74_00640"
     CDS             complement(17572..18750)
                     /locus_tag="PLM74_00640"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HON41624.1"
                     /translation="MSKRRACAASVVLSLVVLAVAAAAFLGLRIPYSSQHGLRRLREL
                     PPDPGDFAAIDLTRADLRALNLRNEGALLRAEASFDSLTAWPIEPWKKPVGFHPRAIL
                     EEGKNPGLGVRELHKRGITGKGIAIGIIDQDLLLSHTEYNERLRLYEVVDGFGYPQMH
                     GAVASIAAGKSVGVAPEADIYYLAIRMDHRKCRNVADAIGRILDINSTLPEDKRIRVV
                     SISCSWLPDEPGYQEADAAVLRAKAEGIFVLSSVVDRYYDFSYHGLGREPGRDPDDVL
                     SYTPGAWWSKESGSWYLPDEDSARLLVPMDSRTVSSELGDEVYAFYRVGGWSWCVPYI
                     AGMYALALQVAGDLTVDEFAKAAIDTAHYTSISLGGTETRLGPIINPVGLIERLASDE
                     "
     gene            complement(18743..>19038)
                     /locus_tag="PLM74_00645"
     CDS             complement(18743..>19038)
                     /locus_tag="PLM74_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41625.1"
                     /translation="APAVWVPMVRHQRFPVQPRDAYKDYTDLPLDRALPRSEAMGCYR
                     LARYGPCDPVAEVSCDPTPGWGNGGGAVKLVSKNPNGHPVRINQGIRGRAPDE"
BASE COUNT         3897 a         5941 c         5039 g         4161 t
ORIGIN      
        1 ccccctggtg tgcgcagact acgatcatgc agtagcgctc cagcgtgttg cctgtgggcg
       61 ttgggtgacg ccgaagccga acccgatcgt ccgccccgac cagcacaact gttggagtga
      121 accctgcctg aatcagctcc gctgcggtca ttcctgcaat catgcgttct gtcatgccgg
      181 gctctatccg aaaacacacg cgttcaaccg cgtccgacac tccacgtccg atcccgggat
      241 accgatcagc ctcccatggg gtaaacggca tcctgagcgc agtcacatca tagaatcgcc
      301 cttgagcagc agtgcagctg aaatcggcgc ccaacttgcc tggtatctca ccaagcattc
      361 catcctgccc atgatgccaa gggtactcaa cgaattcgaa gacatccgcc ggcgcaatgt
      421 gggccagtgt ctcagctctc accctgtcaa tctctatact cgatgtaagt atgtactgac
      481 ggcccggcgt gatcagcacc gatgccacgc ccatgtcggt agtcgaggca caccggctgt
      541 cgcctccggc ggtgatccat gcgaagttgt ccgccctccc cagaagcagc gcgtcaagct
      601 cttgctcgcg catgaactct cgtacgcgac ctagcttgaa ctcaacctca agcctggcgc
      661 attcacctag acagttactc acagttggcg gtcaggcgac cttccctccc tggtacacct
      721 agtacatccg atttttcaga cgggcgccga acactgcata cccgcgctcc gtccgctgct
      781 tcgacggcaa acgccaccgg gaccccggac ggcgattcgc gcctgatctc actgagtcgc
      841 gactgcagta gttcgactct atcccggctg acaaggttaa tagtgcatcc gccaaacccg
      901 gcgcctgtca tgcgcgctcc aaatacgccg ggcgtgccgc gcgcaatctc caccgcttca
      961 tccagttccg gacagctcac ctcgtatagg tcccgaaggc tctcgtggga ctcatacatc
     1021 agctggccga aggcgtgcaa gtctctgtcc ttgagcgccg ccactgcctt cacaactcgc
     1081 tcgttttccc ggacaacatg ttcgcatcgg cgcgctatcg atgcaggcag cacatggctg
     1141 acctgctcga actgctcgga cgatacgctg ctcagcttac acacgtccat acagccgagg
     1201 gcctgcgcga tcagcgcctc tccacgctcc gcctcgccgc gcctgtcgtt atacctggac
     1261 tcgccaagac ctcgccgaat gcccgtgtcg tatacgacca tcgctactcc catttcctcg
     1321 aagaacatgg gcacaagctc gtggtgaaga ttgctgcagt ctagaaagat ggcatgtccg
     1381 gcgcgcccaa taaccgaggc catctggtcc atgacgccac atctcacccc gacaaactcg
     1441 ttctccgcct tctggcaggc aagcgctatg tcgcgcctcg cggcgacctg ctccggatcc
     1501 agcacatcgt cgcccacatc cgcagccgag agcaaggtga acgccgaagc gacctcaagc
     1561 gcggccgacg agctaagccc ggcgcctcgc gggacatcgc caacgatcac tgcgtccagc
     1621 cctcgcaagg gcggaccgcc gctgccctcg gccctggcgc gcaatgccca tgccacacct
     1681 ctgaagtagt tgctccacgg atgaagcgga tcgctctcga tggccgagct accgtcgatg
     1741 ctgaactccg aatactcccc gtaatcggcg gagtatagcc tcactgtgcc gtcatctctg
     1801 gggcggaata ctatgacaat gtcgcggtcg atggccaccg gaagaacaaa ccctccggag
     1861 tagtcggtat gctccccaat caggttcacc ctgcccggcg ctctggcaat cgatatccgc
     1921 gcgcgctcgt ccgcgcccga gccgcgcagc ccggggaaga tgcggtcaaa caactcaacg
     1981 gctcgatcat gctccaaagc caccaggacc acctccactc cctgcagtct cggcagtccc
     2041 gttgatgccc gcgttatcat ccgcgctgga tcgtgcgctg gattgcccgc tcgattgcgc
     2101 gctcgattgt gcgccggtcc gtgcgacggt tggctcgttg gcccgcccaa tcgcagcgcg
     2161 cagctcctcg gccgtctgtt caggaagggt atcgtttatg aacgtgcctg cacccgattc
     2221 gcaccctgcc aggtacttta tcttggtagc agtgcgatgt ggagggtaga actcgaagtg
     2281 caaatggtag tagtcatact gcccgtcatc tgtcggcgct tgatggataa tcatcatgta
     2341 tggaaacgaa aagccgaata gactgtcgta cccacgcgtc accatgctta gaatccgtgc
     2401 gagatggcgc cgctcgagac gcgacagctg cgtaatggac tccctgtgct gcttgggcat
     2461 aacatgcacc tcgtatgggt atctagccga gaagggtatg aaagcagcga aatcctcatt
     2521 ctcagctact accctccgtc cgtccgcaag ctcctcggct agaaggtcgc agaacatgca
     2581 ccgccccctg gcagtcgcgt atttcgacga tgattccagt tccttcgcag cgcgcggggg
     2641 gatgaacgga aatgcgtata tttgcccatg cgggtggtgc aaagtcacgc ccacacagtc
     2701 gcccttgttc tcgaatatca gtacgtaacg cacatagctg agcgcgccca gctcttcgta
     2761 tctatccgcc catacgtcca caaggttggc gatgcggtcg actgacaggt ccgcaaacgt
     2821 ggcatggtgg tcgtcagtgt aaaggactac ctcgcacacc ccttgcgaag gagcagttgg
     2881 ctggagctcg gtaccgggga ctgcgggggt gcaagggtcg cagcgaaaag acggatactt
     2941 gttctcgaac accacaatct cgtaatcggc cgcaggcact tccgtcgggc attctccgtt
     3001 gcgcgtaggg catagtgggc agaatccctc agggggtttg tacgtccggt cttgcctgtg
     3061 agtggccgtc acgacccatt cctcgagaac tgggttccac ctcatctcgg acacccttat
     3121 tcacctcacg tcctgccttg tcattgcaag caaaagagta gaagactaga taccgaccgt
     3181 cttctctgat tatgcgccgc ttctccatat aataaggcct ccccatatat aggcaccata
     3241 agttgagtac ttaaactaag tcatatgcag tattctacgc taagggggca tgttccttcc
     3301 caaagtgcgg gttgggtcag cgattgaccg agtcccgtta gccatgatcg ccaacgtaaa
     3361 gtcaatacgt gaataaagag gtttcatgcc ccgtccgtcg aacatctcac gatgaagggg
     3421 ggctttcggt gagagttctg gcgctcgggg gcgcgggcta tgtaggcagt catgtagtgc
     3481 gggagttgtg tgatcgcggt catcatgtgg ttgtacttga caatctctcc acaggacacg
     3541 agcaggcggt tagcgttgcg agtcacggag cggctgagct ggtcgtgggc gatgtgggcg
     3601 accgaacact ggtcagcgag gtcctgcgct cacgcgagat tgatgccgta atgcatttcg
     3661 cagcggcaag ccttgtgggc gaatccatgg gcaatccgtc caagtattgg cgcaacaacg
     3721 tcttgtgcgc ggttgaacta ctcgacgcca tggtcgaggc aggcgtagac cgaattgtct
     3781 tctcctcaag tgcagcggtg tatggcgagc ccaggcagac gcccataccg gaggaccacc
     3841 ccaagtcgcc caccagcacg tacggcatga ccaagctcgc catagaacac atgctcgcag
     3901 ggtatcacag cgcccacggc gtcaagtcca tatctctcag gtatttcaat gccgcaggcg
     3961 cggacgcctc aggcgacatc ggggaagatc acgaccccga gactcatctg attccgattg
     4021 tgctgaagac agctctcggg ctacggcagc acttacgcat attcggaacc gactacccca
     4081 catctgatgg gacttgcgtt agggactatg ttcacgtgtg cgatctggcc aaggcgcatc
     4141 ttgcagccat ggagcgattg gccggccagg acggcgcttg tgcatacaac ctgggcaatg
     4201 gagcagggtt ctccgttcgc gaggtgctgc gctgcgcaga gaaagtcgtc gggcgcccca
     4261 tccccgcagc cgaagaccag cgcaggcctg gggaccccgc agtgctagtg gcatcgtccc
     4321 aacttgccca gagcgagctg gggtggaagc caacacaagc atcgctggaa cacatcatag
     4381 cgtccgcatg ggcatggcac agcgctgcgc cgggcgggta tggcgagtga actgactaca
     4441 acggaatcga cagaggtcaa tgccgctcac cacggcgcct cgtcgcggga ggcgtcaaat
     4501 acgacattgc cggcggcgat gacgagcttt gggcgcccgg tgagactgaa cggatcgccc
     4561 tcccacacaa ccacatccgc cggcgcgccg caggcaatat gcccggggcc ggccgtaacc
     4621 gcatttccga gtccattctg tgcaaggccg aacatcgcag ccgggccgcc ggtgatagcg
     4681 cgaagcgcgc tctcgcgcgc aaggcccgcc ttcatgcaca gggctgccat taccggcagg
     4741 tattccgatg gaaggctggg cgcgccggtc gagatagcga catcaacccc ggcctcggca
     4801 agcgccgctg ccgtctcgaa cgtggcattg cgcgtttcca ccgacatccg gccgtccatc
     4861 atggggatga acacgaccgg catgcccgat tcgccgagga actcggcgac catgtgtcct
     4921 tccgtcgcgc ctacgattat gccgctcagg tcaaactcct tcgttatgcg cacggcagtc
     4981 actatgtcat tggccttggc tgcatagaat gtaaccggag ttctctgcgc catgacctca
     5041 gcaagtgttt ggagcgtgaa gtcgaatttc ggcctcttcc cactgtccgt tgcctcccgg
     5101 cagccgattg cgtactcctg cgccttcatg aactgctccc tcactaccgc cgctactccc
     5161 attctagttg aagggtattg aacggacttg gagtacgtcg tcttgggctc gtgcccgaga
     5221 gctaccctga gcgatgcgac gcctcgtacc accctgccat ccacaggaca ttcctctgtc
     5281 cgcatggcaa gcgcctgccc gccaaagata ttcgactcgc cgggaccggt aacaaccgtg
     5341 gcgactcctc cggccaaagc gcgcccgaag ttggcatcat cccagtcgac ggagtcaagc
     5401 gcgcgcaact gcggctgaac agtcccgttt gagtcgctca ggtcacgtcc ctccgagcct
     5461 gctcctgtgc accaaagccc catatacaca tgggcgtcta ccagacccgg agtgacatag
     5521 gccccgtcca cgtcaatgca ctccaaatcc tccggaagct caaccgccct atccacactc
     5581 actatcgctg catccaacag caagctagtt tcgccgacct cgcgaacccc atcaaatagg
     5641 actgcgttct tgatcgcgat cactttctaa acaccacctt gccgccgatc agtgtcatct
     5701 ccacgtccgc ctgcagtttg aaaggatggt ccgtcatcac tactaggtct gcatccttcc
     5761 cgggctcaat gctgcccaca cggtcgccga ccccggctat ctgagcggcg tttatggtca
     5821 ttgcccgcag agcgtcgcgt tcatccattc ctgccctgat agccatcgca gctgaatatc
     5881 tggcatgcct caggggcgtg aacgggtgat ccactgtgag cgccactttg accccggcct
     5941 tggcgagcac tccgggagta acaaatgacc agttgcgcgt ctcgctgacg cctctgggcc
     6001 gaaagaacgg gcccaccaca cagggcgccc cgctatcaac tatttcccca acgacgaggt
     6061 gaccgtcggt aacatgctcc aatgtgaagt ccacgccgaa ctccttgcac agcctgatca
     6121 ctgtgagtat gtcgtcgcct ctcgacacgt gcaccctcgc cggcatcttc ctctgtagca
     6181 ccatcgcgag agcctccatg cccaggtcca ggggtgtgga cgtatctgcc tctttgcgct
     6241 cagtgtagtc gcgcgcccgg tacatggcct cgcggaatac ggccgcaacc cccatgcggg
     6301 tggttgggtg attctccgct ttggtcttgt atgggttctt cggattgcct ccgaaggcga
     6361 acttcatgcc ggccggatgt cgcagaacca tctggtccgc aacagtcccg ctcgtttttg
     6421 tgaccgtgct ctgcccgcat atcacgtctc cgctgcccgg cgtgaccaga acagtagtga
     6481 acccggccgc gggcgcatcc tgaaacgcga tgtcgaatgg atccagagcg tcgagcgctc
     6541 gaacctgcgg aacagcggga gacgacatct cattggcgtc tgaccaatac gtgtcttcgc
     6601 cttccacgaa aagcccaatg tgacaatgag gatccacaaa tccgggcagt acaaccctcc
     6661 cggcgcagtc gatagtctca cagtcttccg ggatctctat ccggctccct acatccagta
     6721 tcctgccgct gtcgattagt actgttccct cgtcgatgtc gcagttcgcg actggtatca
     6781 ctctgccgcc tgtgagtgca agcaacattg ccacctctcc ttcacagagc tatcagttct
     6841 ttcggcgaga gggtaagcac ttcgcagccg tcctccttca cgaccaccgt gtcttctatc
     6901 cttacgcctc cccacctcgg cagatagatg cctggttcga ccgtaacgac gctgcctgcc
     6961 gcgagcgtcc tttcgcactt tggagacata aacggctctt catgaatctc cagtcccacg
     7021 ccatgtccca ctccgtggcc gaagaagtcg ccgaaccctg caccctttat gacatccctt
     7081 gcggcccgat cgggatctgt ccccgcaacc cctgccctga tctgcgaaca tcctacagcc
     7141 tgagccctct tcacagtctc gtagatctcc ctctgcgtgt cgtttgccct acccatgacg
     7201 atggttctgg tcatatcgga cctgtagccg ccgtacatcg ctccgaaatc cattgtgacg
     7261 aagtcgccgt actcgatttt gcgccgcgac ggaacagcat gcggcaggga cgatctaggg
     7321 ccagatgcaa cgatggtgga aaaaccggct gcagcagcgc cggctttctt catgaagtag
     7381 tctagctcga tttctatgtc cttttcctct atccccgtct tcacgatctc aagtatgtgg
     7441 tgaaacgcct cgtcggctat cctggccgcc ttggcgatga gctggatctc gcggtcgtcc
     7501 tttacgtacc gcagcgcctc cacaatcccg gcagtcggaa ccaactccag gccggaattg
     7561 gccagcctgt aatagaggct aaacatgata gagtcttttt caaatccaag gctgcacact
     7621 ccatgctttg ctgccagtcc cgccaggaca gtctcaagct cgggaaatgg atgccgatag
     7681 cgaactacct cgaaacctgc gcattcatct tctgcctgct ctatgtaacg cgaatcggta
     7741 atgaacactc tggccgtatg tgacaagaac aagaatgatt ccgagcccgt gaatccactt
     7801 agatatctga cgttcggctc gcttgtgacg ataaggccgt caacgccgtg ggtttccagg
     7861 ttgctgcaga ctagctcaca ttgtttcatg gataacccgt acccccttat gccgcgtccg
     7921 caagtatgta ttgcacgacg cgtgccaacc ggcggcgagc gaaggccgaa agcgcaaact
     7981 ggcctatgca gcgatgggag tcagagaaag gagtgcatgg gggcccggac ttgacccagg
     8041 tttctcaaat gagaagaacg tccgtatcag atgagaaggc ccgagaaaga tctagaggcc
     8101 gtattcccga agcctgcgat acagagtggc tctgctcact ccgaaggcag ctgcgatggc
     8161 ctccttggcg cgagtggatg tcccgtagcg ctccagcgca tcctcgagag cgctgcgggt
     8221 catgcccggc ttcgcggcaa aaccgatggg atactcccgg tcgtatcggt ccgggccaag
     8281 atcgggtcga gaaccgggga cggaaccgga gcccagagcg gcaccagcgg aaggagggca
     8341 aaggacctgg aacggcaggc aatccgtatc gatgacagtg cctgtcgcaa ggtttaccgc
     8401 atattcaagg gcattagata gctctctaat gttgccgggc cagtcgtaac tgaccagctt
     8461 gaccatggcg gcgggctaca ctgacgtcac gttcctgcgc aggtcgtgcg catacttggc
     8521 aacaaagtgg tctacatact cggggatgtc ctccttgtgg tcacgtagcg gcgggataac
     8581 gatggggatc acggctagcc ggtagaacag gtcgggacgg aatccgccgc gcatggacat
     8641 cttgcgtagg tctttgttgg tggctgatat gatacgtatg tcgatcggga ccgctttcct
     8701 gccgcccagc cggtctacta cccgctcttc gacagcccga agaagcttac cctgaagatg
     8761 gagaggcatg tcgcctatct cgtcgaggaa gagtatgcct ccgttcgcaa gctcgaactt
     8821 gccgggcttt ccgccccgct tcgcaccggt gaaggcgccc tcctcgtaac cgaagagctc
     8881 gctttcgaga atagactcgg ggatggcagc gcagttcacg gcgacaaacg ggccttgtcg
     8941 gcgagcgctt tcgtggtaaa tggcccgggc gagcagttcc tttccggttc cgctctctcc
     9001 ctgaatcaag acggtcgagt cgccttcagc gatacttgcg gcgatgctct tcacgctggc
     9061 catcgccgcg ctggagccca gtatgtcgtc gaacgtggaa tgtcgttcgc ttcggatgta
     9121 attcgcgacc atcctgggga tctcctcgat cccgcggaag gtaatcacat gcccgcgagg
     9181 acagaaatca tctgcgatcg gcctcactgt tgtgagccag tattccctgt tgcctctgac
     9241 gcatgagtag gtctcaaact cagacgaact gacttctccc agagtatccg aagacaactg
     9301 ggggaaaata tcacttaccg ggcaggacgc cagttgatcc cgcgtcgact taagcaggcg
     9361 agcagcagac tggttggcat ggaggatcac cccggagtcg ccgatggtga ggattccgtc
     9421 attcacggag tcaatcacga catctagctg ccttgccagg ccctcggccc ttgcagtctg
     9481 ttgcctctcg ttgaacgccc gggaaatcag atccgccatt ctttcgagga agttgatcca
     9541 cctctgcatg tcgccaatga gaatctcctt ttgcgaatcc gtacaggcca caaggatgac
     9601 gcagccggcc ggtcgcccct cgatcatgat ggggcagata atgtcggcga tgtcagtaca
     9661 tttgtccttc tgcgtgcatt ggcggcatac gtcgtcatac tgcggatttt cgatgaccat
     9721 agtccgcccc tcagcgagtg cccgcgctga tacactgctt cgctccagcg tgcggcctat
     9781 atgccgggca tacttgcctg aacctacgac ggtcagcagg ttctcgtcga ccatcgctac
     9841 ttcaactccg gctacggcag cgatcgcgtc cgccacagcc tgcgcaaatc cctgcacctc
     9901 cgggagaacg gtcatggcga tcagccctct acggcaatct ggatggagca cccattcctg
     9961 agatcatcgt acatgttgtg cctcaccctg cttgtaagcc attctacatt cgctcaccag
    10021 ttcccttccg aaacgatgcc attacgtgcc gcggtgctct tacggctcac ttcgccaggt
    10081 ggcaagcaac caaatgctcc ctgcccacct cgctgagggc gggttcgtcc tctctgcaga
    10141 cttgcgtgac gaatctacac ctcgacgcga acctgcatcc cgccggagga tttatggcgc
    10201 ttggaatctc gcccttcatg acaaccctct ctttgctcct ttcctcatcg gggtcggcaa
    10261 ctgggattgc ggacagaagc gcctgagtat aggggtggag cgggtttctg tacagctcct
    10321 cgctcgttgt gagctcaacc agcttgccca ggtacatcac gcccacccta tctgaaacgt
    10381 acttcaccat ggccagattg tgcgatatga aaagataggt gaggttctct tctcgctgca
    10441 gcctcagcaa gagcttcgtt atctgggcct gaatagacac gtccagagca gacatgggct
    10501 cgtcgcaaac gacaaagtcg ggctcaacag ccagcgcccg cgcgattccg atcctctgtc
    10561 gctggccccc tgagaactcg tggggaaacc ggtttatgtg ctctctgttg aggcccacag
    10621 tgtgcaggag ttcgcagacc ctctcttcct gttctttgcc gcgatagagt cggtgtacat
    10681 caatcccctc cccgatgatg tccttgactg tcatcctcgg gttcagagac gagtatgggt
    10741 cctggtagac aatctgagcc ctcttcgtga attccttccg ttcgctgggc tcaagcctat
    10801 gcaccttcat gccatcgaag actacctcac cctccgtcag gcttagcagt cccagtattg
    10861 ctttgccgca cgtagtcttg ccgcatccgg attcgccgac aagccccaaa gtctcacctc
    10921 ggtaaatcct gaaactcacg tcgtctacgg ctttcactac tctccttcgg ccggcctgga
    10981 agtacttctt taggcccttt acctcgacta gcacttcatt acctggcatg cgcctcactc
    11041 cttccttgaa gcacttccgg cactccagtg atgtcggcgt ctggatgatg caaccagcaa
    11101 taggatctct gggtttcgcc gcatgacacg ccggcgggag gctcaagttt gcagatcttc
    11161 atggcaaact cgcatcttgg cgcaaaggga cagccctgcg gagggtttat caggtcaggc
    11221 ggagtcccca ctatcgaata caggggctct tgctttgtgg agcttaattc aggcacagac
    11281 ttcatcagcg caaacgtata tgggtgcttc gggccgtaga agacttctct gacgctcccc
    11341 tcttccacga tcttgcccgc gtacatcacg tgtattctct ctgatatact ggccactaca
    11401 cccatatcgt gcgttatcaa gatcacgccc atgtcccttt cgcgctgcaa ctgcattatc
    11461 agttcaagaa tctctgcttg tattgtgacg tctagagccg tggtcggctc atcggctatc
    11521 aatatctccg gattgcacgc caaggctaag gcgatcatga ctctctgcct catgcctccg
    11581 gacatctcaa acggatactg gttcattcgt tccttcgcgt tggcgatctt gacgagttcc
    11641 agcatctcca gggcttctgc tctggcctct gcgtgagtct tctgctcgtg aatgataatg
    11701 ctctcggcga tctggtctcc gattctcatg gtcgggttaa gcgcagtcat cgggtcctga
    11761 aatatcatcg aaatgtgttt tcctctgatc ttcgtcatct cgtcctcgcc gtactccagc
    11821 aggttctccc cgtggaaccc tacttcgccg ccgatttcca cctgttcgtt ggggagcaat
    11881 cccataaggg ccttggcagt cactgtcttc ccacagccgg attccccgac cagagcaagt
    11941 gtctcgccct tttcgagctc gaagctgacc cccctgacag cttgcacaat gccggcgtat
    12001 gtcttgaaag acacaaataa gtcctttgta ttcagcattt gcccgtttct catgcgtcga
    12061 cctacctcct cagtttggga tccagcgcgt cggacagccc gtcgcctatg aggtggaatg
    12121 agagtgtcgt aaggacgatc atcaggctgg ggaagaaaag catgtgaggc tgaaacatca
    12181 gcttttcctg gccgtacgac gccaaggcgc cccaactggt ctctggaggc cttatcccga
    12241 ggcctacgta actgagaaac gcttcgccga atatgaaacc cggcacggac attgtgatct
    12301 ggaccatgat aatccccaac gtgttgggga ggagatgcct catgattatc cgccaggtgt
    12361 ccgaacccaa cgcccgggcg gcttcaacat agtcctgctc cttaagctgc agtatctgcc
    12421 ctctgaccat tcgagtcgtc ccgacccatg aggtaaggct catggcgaac accagggcga
    12481 agaggctccg tcccatcacc agtgtgagaa ttacgacgat gatcaagtag ggcagattcc
    12541 ccacgaactc gaccaacctc attatgagat tatcaaccca gcctcccttt agccccgcaa
    12601 gtgcgcctag ggtgaagcct attacgatca tgacgactgt gcacaagagg ccgataagga
    12661 tggatactct tcctccgtag catacccttg tgaacatgtc tctccccaag tcgtccgttc
    12721 cgaaccagtg ttcagcgctt ggcttttggt tcctgatgga ggagtctatg tattgatagt
    12781 ccttgcctga caatgcaggc cccaagaaca gcatcacaat tatgatcacc agaaggccca
    12841 agccgaagac tgccagcttg ttctgcctga accttcgcca tacatccgcc cagtaagtga
    12901 gggcaggttt ctccaggtgt tcggaagata cctcatcgac accgactcgc tcgaacacat
    12961 cgcccgccag aaccgtacca tccacgttca tcgctctccc cctacactgt cggtgctcct
    13021 gctgcttatc cttcagttcc cggaatcctg attctgggat caactacgca gtacatgatg
    13081 tccacagcta tcaacgatgc aacgtacaac acagcgaaga acactgttag ccccagcacc
    13141 atagtgtagt cgctgtcgct gactgctttc acgtaatagc gcccgagccc aggtatcgag
    13201 aagacttttt cgattatgaa agacccgccg aatatccccg ctatggaagg gccggtcatg
    13261 gtgacgatgg ggatcatgga gttcctcaga acatgcttct gcaggatccg acgcctattg
    13321 cagcctttgg attttgccgt aacgatgtag tcttcgccga ctacagatag agcgctgctt
    13381 ctcatgtatt tggcatagac ggcaattccc ccgatcgaca tggtcagcac aggcattatg
    13441 taatgctcgg gcctgcccca accgaatatc ggcgccagtc tccacttgaa cgcaacgtag
    13501 tactggagaa tagcggcgaa cacaaagagc ggcgtgcata ttgccagcac tatgcagact
    13561 ctcaggacat ggtccgcgag tcttccgtgg ttgaatgcca tcagtaaccc gaggataatg
    13621 cctatcacga ggtcgagcgc aagcgctatc gcgcccaccc ggcccgatat gggggcattc
    13681 tcccggatca cgtcattcac tcttctgcct gtgtatacaa tggattgccc gaaatcgccc
    13741 ttcagtatca ggttcttcat gtatctcacg tatctttcga gaaccggcct gtcgagcccg
    13801 tatttcgccc gaatcacctg ctgcgctctc tcgggcatct gctcgacctt agccgctatg
    13861 ggatcgcccg gcgccccggc tatcagaaag aaggtcgcag tgatgaccag gaacagcgtt
    13921 actagctcgt aaactaccct cttcgcaaga aatctggcca tggtattccc cctctatatc
    13981 gggtcgcaca tcaggtgcga tcttaggcgg aaaacaaggc ataagcctgt cggttcgccc
    14041 tcatgccttg tcttccgcat cacgcgagtg gactgccggc ctgtgcgcgc tctatcctgc
    14101 cccttgccca gtccctaccg gccgggtctg ccctttgtgt acagcttggt atagtcgacg
    14161 cctgcgtgtg gattcgtgta gtagccgttg acgtagttgg ctacgtagta tgttgccacc
    14221 ccgcagtagt tgggagcgat tacgcccgtg ccgaccagca gttgctcagc ctgcagaagc
    14281 agttcagctc tggcctgcgg gtcaacggtg aacgaggcct tatccagcaa ttggtggtat
    14341 ttgtccgcgt cgggaccggt ccaccccgat ctcttactgt ggaagtaacc gctgatggga
    14401 tcgaagatct caaggagggc attggggtca ttgtagtacg gaccccaccc ggacgtgcag
    14461 atatcgtagt ctcccttgcc aaccttgtcc cacatgatgt tccattcaac catatctatg
    14521 gtaatcgtga ccccaagtgt cttctgccag ttctgcagga gccattcagc ggatttcttg
    14581 gagaactcgg cagtgcctct cgtcgcatac tttatctgca tttttgccgg attcggatcc
    14641 agtttctctt ccttgaggcc ctcaatcagg agcgccttcg ggtcaggata cttcgtcatc
    14701 agcgtcttca caatctggct cttgccgcct accctctcgc tgtagagctt gtcgccgcac
    14761 atggtggccg aaggcatcat cgaatagaga ggctcggccc tcccatacag cagatcctcg
    14821 atgtaccttt ctctgtcgtg ggccagtgag aaagccagcc ttatcttggg gttcttgaag
    14881 tacttgttgc tgcaattgaa cccgaagaac tcaggcgcat tgtccggctc cttcagagtc
    14941 ttgaacctgg cgccctcttt cttgagcact tcctgccagt cggggtcgct ggttgccccg
    15001 atatcgatct ctccgcttat caacgcctgc acgtgcgtcg cagggtcctg tatgatcttt
    15061 ctctctatgc gctggagagt gaagttcgcc gcatcccagt gcttcgggtt cttgacgaaa
    15121 ttcatctgca ccatcggttt ccagctctca atgaggaacg gaccattcat cacgaccttg
    15181 tcgatggacg aaccgtaact ctctccccac tgctcgtact tgtcttgcct cacgggcttg
    15241 actcctgcga gcaggtcggg gaagtaggcg catggtttgg tcagcttgaa cgacacggtc
    15301 ttcttatcaa cggcggtcac gccgatgtcc tgcggtttct tgccctcgtc gtagtgggcc
    15361 tcctcgaaat tctggatcac gccgtcgaag agccatgcat tagtgcagcc tacgctgggg
    15421 tcagccatca tccggaagga gtagacgaag tcgttggcag ttaccggaac gccgtcgttc
    15481 cacactgcgt tgtctctgat cttgaaggtg tacacagtgc cgtccttgct gagatcccac
    15541 gatttcgcgg acgtcagctt tatgaccgca ttgccctttt cgtcggctac tctttcaacc
    15601 aggccctcta ggatgatgct ctgaacaccg ttgtcaggac ccgtggtgtg gagcagaggg
    15661 ttcagccctg taatatcgct cctactgctg aatacgaact tctggacctt atctagtcca
    15721 cttgctgtgc tcgcgtgtgt cgcgcctcca ccaaaaaccc cggcacacaa caaagcaatc
    15781 gctacgagga taagggtccg ttttctcacg cttatgccct ccttatgcac tcaagatggc
    15841 ccgtatgcgg ttacgcaggg accgtccctc cgtaccgcac acgcacatgg gacgcaaagt
    15901 cgcgtctctg atagaaacct cttcttctcc cggcgttgat ccgcatcaca cagtcttctc
    15961 gcctttcacc ccctcacttg ggcagatcga cgtcgcctct tcgcagcgac agcatcttta
    16021 ggcccgcgtc tgttcgtaca tcaacgacac aatgatgtat tgcatgacac gtgccatcgg
    16081 gcagcaggca aacgcccgtg gcggcgaaac cggcgcagtg cagcaagaaa gccccaccag
    16141 aagcagtgcc gagaggctcc caactagtcg ctggtttctc aattgagaag gaattgggtg
    16201 tcaaatgaga aggcttgaga aatgtgaagg gggcgaggcg ctcctcgtga ttatcatgct
    16261 ccagtagtcc agcctctcca gcgtaaactc gatgatgcgt ccgcgcccac aaggttgact
    16321 agctgaagca acttgtatcc atgaccatgc ctcacagtgg cccatcctgg ctacgttccc
    16381 ttgcggttat cgtgccaggc ggcgatcctg gcagactagg ctgccgccag accgctaatt
    16441 gctctcgcga tgtttgcggc gcccacggaa tccgcgtgct ctgtgtggcc gcaggcggta
    16501 cacccaaagc gaatccccct gcggctggct tggtcgacga cgccacacgc tggaacgggc
    16561 ttccttgtcc ttcatgcggc ggatagcgcc tagagctttg gccttgccca tacgacggcg
    16621 caatgccttg aacctgcggc ggacaaaggc ggcgtggtcc ccgctgaaga acagcgtctc
    16681 gccttcgcaa ttgaccaccg ctaagttcgc gaggcccagg tcgacccctg ccaccttcgt
    16741 cccgacgcac ggagtcacct ggacggagat ggacagagcc aggtaccagc gcccacgctt
    16801 ctcatacagt ttagctgcgc cttgcttgac ggttgcgcca agcttggcgt tgagcatctg
    16861 tgcgagctcg ggcatcgccg cattgcagtc atagctctgg actttgagag taacgtgaat
    16921 caggagcgcc tgagaggatt ccacagcggg ctcaaactcc acgacatcga acccgtatcc
    16981 gacctggaga tttcacttgc cacttcagaa aaggagaacg gctgcttcga ctacagcgct
    17041 gagcttgaca agagcccggt aacagcgcac aggcggtttt ccgagatcgt cgaacgcctc
    17101 atgtcttcag acaaacctcc cacagccgtg ttctgctttg attaccacac cacgcttctt
    17161 ctgatccaga gtctcatgag gatggggcta acccccggaa tgcaggtatc aacaatcgga
    17221 ttcgacgctc caagaacagc gtgcgccccc cgggtgacaa gcatcaatca gccggctctg
    17281 gagatgggcg ccatcgcggc aaacctgctc ttagaacgca taagcgatcc aacgctccag
    17341 ccacggacag tgcgcctgtc cgcacagctt gaagtgggcg acaccactgg cccgccgacc
    17401 acatgaggtt ggcagcggac tgcagcatcc agaagagcaa cccgcagaaa atggccgccg
    17461 ggcgctgcta tgtacagggc aaccacgaag tcggcaaggt tgggaagccc ggataaaact
    17521 ggcacgctcg gccacgctga cgtcggtcgg gtacgacgac tggggcaaga ttcactcatc
    17581 cgaggcgaga cgctcaatca agccaaccgg gttgatgatc ggcccgagcc tggtttccgt
    17641 tccgcccagc gaaatgctgg tgtaatgggc cgtatcaatc gcggccttcg cgaattcgtc
    17701 aacagttagg tcgcccgcca cctgaagagc cagtgcgtac atgcctgcga tatacggcac
    17761 acaccaactc catccgccca ccctgtagaa ggcatacacc tcatccccaa gctcgctcga
    17821 tacggtgcgc gaatccatcg gaacgagtag tcgtgcagaa tcctcatccg gtaggtacca
    17881 cgagccagac tccttcgacc accatgcgcc cggcgtgtat gacagcacat catcgggatc
    17941 gcgtcccggt tcccgtccta atccgtgata gctgaaatcg tagtagcggt cgacaactga
    18001 gctgagtacg aatattccct cggctttggc tcgtagaacc gctgcgtcag cctcttgata
    18061 gccaggttcg tccggaagcc atgaacagga tatcgaaacg actctgattc tcttatcctc
    18121 cgggagagtg ctattgatgt ccaggatcct tccgatcgcg tctgccacat ttcggcattt
    18181 cctgtgatcc atcctgatgg ctaagtagta tatgtctgcc tccggcgcaa ccccgacgct
    18241 cttgccggcc gcaatcgacg ccacagcgcc atgcatctga ggatacccaa agccgtcaac
    18301 gacttcatac agccgcaacc tttcgttgta ctcggtgtgg ctgagcagca agtcctggtc
    18361 gatgatgcca atggcgatgc ctttaccagt gatacctctt ttgtggagtt cgcgcacccc
    18421 gagcccggga ttcttgcctt cctccagaat agcccttgga tgaaacccca cgggcttctt
    18481 ccatggttcg ataggccacg cagtgagcga atcgaaactg gcctcggcgc gcagaagcgc
    18541 tccttcgttc ctcagattca gagcgcgaag atcagctcga gttaggtcta tagcggcgaa
    18601 gtcgccgggg tcagggggaa gctctctcag ccgtctgagt ccgtgttgtg agctgtaagg
    18661 aatcctcaat cccagaaacg cagctgcagc tacagccaac acgaccagcg ataggaccac
    18721 acttgcagcg catgctctcc gcttactcat cgggcgccct ccccctgata ccttggttga
    18781 tcctgacggg atgtccgtta ggattcttcg acaccagttt cacagcccct ccgccattgc
    18841 cccacccggg tgttgggtca cacgatactt ctgcaactgg gtcgcaaggc ccatatcgag
    18901 ctagtcggta acaccccatg gcttccgaac gcggcagggc cctatcaagc ggcaggtctg
    18961 tatagtcctt gtaggcatcg cgaggctgaa cgggaaaacg ctgatgccgt accatcggta
    19021 cccaaaccgc gggagccg
//
LOCUS       DAOTAC010000007        18847 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_2426_length_18847_cov_4.442369, whole genome shotgun sequence.
ACCESSION   DAOTAC010000007 DAOTAC010000000
VERSION     DAOTAC010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 18847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18847
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2426_length_18847_cov_4.442369"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="PLM74_00650"
     CDS             <1..637
                     /locus_tag="PLM74_00650"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HON41626.1"
                     /translation="AGVLHLAVNSYSLHNLGNVVERLFGTPRFALIYLIGGVWGSLAS
                     TFMSDPRVLGVGASGAIFGLAGCLFYFGLRYPNHFRVLAGWRFIAIVLINLLIGFSSS
                     HIDGYAHMGGLVGGFVAALALGFPTERTSHVRSTARICLAIATLGAIVLAVRPTPASI
                     FVPISMSNPGSKSVSAAILACGGVQIRWSAQPHQARSHLLGSKAAMPADTK"
     gene            2753..3949
                     /locus_tag="PLM74_00655"
     CDS             2753..3949
                     /locus_tag="PLM74_00655"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HON41627.1"
                     /translation="MGVFDKRIDTFLAEYNDLREKDLYYYLQQVDQLDGAHVWIGGRR
                     MVMFSSYSYLGLLKHPKVQERAREALDRFGTGTHGVRILAGTTAAHVECEQRIASFLG
                     TEDAIAYSSGYVANLSTISTLLGRHDVVITDKLSHASIIDGCMLSSAEFQRFKHNDLD
                     DLKRLLEADADRFEGKLVIVDAVYSMDGDVCPLPELVALCREYGAWLMVDEAHSLGVL
                     GKTGHGIIEYFGMSPQDVEMLSGSLSKTLPAVGGFVAGRHDLIQFLRHTARGFVFSAA
                     LPPAAVAAITASLDVIEEDPSLIAAVRRNIQRFVGGLNEMGYDTLNTKSCVIPIIIGK
                     PEPTLELTSRLHRDGMFVSPILHPAVPMNTCRLRANVTAGHTDEDIDFALDLLRRHGK
                     DMGIIK"
     gene            3946..5181
                     /locus_tag="PLM74_00660"
     CDS             3946..5181
                     /locus_tag="PLM74_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HON41628.1"
                     /translation="MKAVQFEGTVPRYVYSTVAGAINRNAYYDRFSNIVLRDVRPPEL
                     PTPEWVRIRTKYAGFCGSDKNLVLLHDSPSTSPYASFPFTIGHENCGYLSEMGAVAAE
                     RLTVGIGARVLVDPLLSCVPRGISPVCPSCARGDYSLCTNFTEGSVSPGMSIGTCRDT
                     GGGWSSEFVAHYSQLIPLPDSISFEDAVLVDAFCSSLHPVMRNFPSDDSTVLIIGCGV
                     IGLCAVAAIRALGGKARIVVLAKHEFQGEAATRLGADAVVYMANSRDYYAELADILRT
                     KLLKPMLGKRVVVGGAHTVFDCVGSSTSIDDALRFTMPHGTMVLVGLAAFPKGVDWTP
                     IWLKEVQVRGSFWCSTEQFEGKAMRTYEIAVELLRSGRLSLSHLLTHKFSLEEYKQAI
                     EANLHLSKSRLIKSAFEFD"
     gene            5361..6710
                     /locus_tag="PLM74_00665"
     CDS             5361..6710
                     /locus_tag="PLM74_00665"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HON41629.1"
                     /translation="MARVGITDTTFRDAHQSLFATRMRTHHMLEIAAYLDKVGFHSME
                     VRGGATFDTALRYLNEDPWERLRLIRETVVNTPLQMLLRGQNLVGYRPYADDVVDAFV
                     GKMVELGISIIRVFDALNDIRNIERSAASVKRFGAHLQGTISYTVSPVHALDGYLDYA
                     RRLKDVGADSICVKDMAGILTPYVAYDLVRRLVDEVGLPVHLHSHYTSGMASMTYLKA
                     IEAGAAVVDCATSTLAMGTSQPASESLIVALAGTGYDTGLDARLIRPAASILRRIIDD
                     TPEYRDLIGNMQVNTDVLVYQIPGGMISNLRAQLRQVQADSRIEEILAEVPRVRAEMG
                     YPPLVTPISQIVGAQATFNIIQGQRYKSISNEVRAYVRGEYGRPAGEIDPSLKRMVLG
                     DEKPVISRPGDGLTPQMPTAREAVAPYGGSEEDAISYALFPKETENYLAHRASHPRS"
     gene            complement(6695..7336)
                     /locus_tag="PLM74_00670"
     CDS             complement(6695..7336)
                     /locus_tag="PLM74_00670"
                     /inference="COORDINATES: protein motif:HMM:NF036525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HON41630.1"
                     /translation="MSEIAGAEEQWLGMNRTGRKREICRGAARVFAQKGFESTSVDEI
                     AAHLGVAKGTIYYHFGGKRELFAAMISDGLERLLVDAERSIEKVDDPVEQLSRVLDVL
                     LEHGRRHIDFMRLFFREVLASGREWLTEVAEYRDRFAKLVGPIITRGKAEGRILNIDT
                     DTVIRYVLSALATAVLDLGDGSLPPAGSARLIKRMVMSGISCDPATAGIYDRG"
     gene            complement(7333..9375)
                     /locus_tag="PLM74_00675"
     CDS             complement(7333..9375)
                     /locus_tag="PLM74_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41631.1"
                     /translation="MKKTRQRSVLCLCALLVLIAAGRPAIGRQSAALAEATVGSRSST
                     ATGEAGSGTLSRIRDYETVFAVLAPDGQVMSTTVVDWIRSPEGGPTSIFDPGDLTDVV
                     NLRGPEVPQVSETGLTWGSDPSQTTDINYSGRSSRPLPVSIKAAYILNGTEVPPEQVV
                     GSSGRLEIRLSLHNTTIRSQKLQHGAFGSPGASVAFSADVCVPMIVQISSDVPLPSYR
                     CIDAPGATTVLAGKDLKVNWMLFPAPDASASLILEGDDMSPAGFDVSIIPALPLVSQL
                     DIVDQLREFAAGATALDAAVREAENGASRLAEGQHELEKGIAALEDGLRDLARLADAH
                     YTIARTINDGLAGQVLEAPLELAQLAEADLAILTQIAEGVGSVVSMLPADLLGGNAGF
                     AERVVSGVTDSDAAAQTLSRALASQAQTVASAKSAGSDAMAAAEALARANPSIAKSSE
                     YAALSAALKKQDAAVNMLSAGGRVGFSQVPSLPQLAETARGLASNTARLKLGTSLVSG
                     YLSQSDEMIAQAMQAVAALNAVVYGGEVEGNTIPGMGVLCEGLRQIDAGLNDLKRGTV
                     LLAEGGSVEGAELPGMSTTVEGLTAASSGVGTIRGGCEELASGARQLAGGLARMRKEG
                     TSRMADELSKGLAEAEKGQAVLAAMADRLAGYDSFIGKPEGAIGEVRFLMKIKAAI"
     gene            complement(9450..11621)
                     /locus_tag="PLM74_00680"
     CDS             complement(9450..11621)
                     /locus_tag="PLM74_00680"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HON41632.1"
                     /translation="MRWGVRLLDLVNSIPRFTVRHRAAILIAAAALLVPAVYGTLNVP
                     IEYDLLKYLPRNLESVRGYEVLDREFRYAERAAVVVKGQPDWAVLSIKDRLSKIDGVS
                     SVFWLSDLADPTIPHDYLGEELVSQFYRGDEGDITFLLVSLAEDSSSPRTVRAIDQMR
                     RLLGEDQYLVGEVVSGIEMKELGMSQMPKMIRTAAVLIFLVLIVTLPSAAMPFIFMIT
                     IGAAYAYNMSISYALGQKVSYLTSSVAAAMQLGVTMDYCIFLMHSFHAERNQIVVGAS
                     TGGTRRDGADAEAAVERAIVKTANAILSSALTTAAGFAALALMRVRLGADMGLLMARG
                     VLLSVVCTLVILPSLVLALQNVIDRLTHPVLLPSFRGLASWVVRRRFPVFAMALLVLV
                     LGYVGSSRMSLSYDIESTFPRDMPSIAAMEEFGDVVSGLREMYVVAGGVPGWRLQRIT
                     TALRGIRGVVGASCLADAVDPAVPIDFVPKGVASRYVAGEYSAAIVSIDSKASDEATN
                     SLIADIREMMPRVLDESDATVYLTGGPVISSDLMRLTSHDISVVNIVSVAAIFIIIAI
                     AFRSLTAPVVLVAAIQCAIWLNQSISYFTHTPLFFFSGLAINAIQLGATVDYAILMAS
                     TFRGKLSTCSPTHAMRLAVEESGPAILCSGGAMFAAIMGVYASTSLRAVKDLAVLLAR
                     GAIVSTIVATVLLPAILLVVQKVLAKTSAGWPEGTDGGPDA"
     gene            complement(11812..14217)
                     /gene="ccsA"
                     /locus_tag="PLM74_00685"
     CDS             complement(11812..14217)
                     /gene="ccsA"
                     /locus_tag="PLM74_00685"
                     /inference="COORDINATES: protein motif:HMM:NF027651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HON41633.1"
                     /translation="MTTYSSWNYALIGAFVASCFSLVFYFMSSSARTAAMRERRSRLA
                     RGLFAVEFILLLAASAALFSAFLKHDFRLAYVASYSSRGLSAFYLVSGFWAGQEGTFL
                     LWAAISGAIGLILAASASHTAAGARIPANTGASSYSSSAVPDAVVSAGAEASVMRFYV
                     LVHVVLMVLLLRATPFRLLPAVPADGAGLNPLLQDPWMVIHPPIVFIGYALYAVPFAY
                     AISALARDEYSEWAKPALAWTVAAWLFLGAGIIIGAKWAYATLGWGGYWGWDPVENAS
                     LVPWLAGAALMHTLIVQRERGKMVRTNIVLAIVSFLLIVYATFLTRSGVLSDFSVHSF
                     ASLGLSSYLVAACVLFATLSLWMLVWRWRSMTSKRNYREPYDQTVSRDFAFFLTALLF
                     VASAAIVSLGTSTPLLTSFSGNPSSVGQAFYNMTNAPLGFMLMLTVAVCPHLAYGAST
                     LAQVGKAMLIPAVAAIVLTVVAVLLGATGVWFVLFLFAAFMALAGNLVVLVKRIRARM
                     SLRVMGGLVAHLGIALILIGVIATSAYNRTETISLQAGEELTVFGWRVAYVSRDEFEA
                     AGSHRPSVAWNLEVSKPGSGTAITAKPYMRPTNQGMLRHPAIVSTIASDLYISPLQED
                     ASREPSWADNAKEVRLHKGEQVEVDGLAVKFVGFDMSQHLGENVMAVGATLEVLDISS
                     SRELGTVTPVLGFAAGGQMQTDALIDLDAADSANPGDAAGTRSVAFRLTSIDAGAGMA
                     VLRIGKLLPGDDVQGNQSGQQSVTLEISVKPFASLLWVGSVLLLAGTAVAVVRRAKEA
                     SAR"
     gene            14559..15296
                     /gene="pyrF"
                     /locus_tag="PLM74_00690"
     CDS             14559..15296
                     /gene="pyrF"
                     /locus_tag="PLM74_00690"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HON41634.1"
                     /translation="MNSKDQIILALDVDTEEEALSLANRLSGHVGAFKVSLRLFSIAG
                     PQIVGRLQEMGQVFIDLKLHDIPSTVAAAGRALTRLGCSMLTAHAAGGTVMLRELANA
                     VSEESRKTSKPAPTVLAVTVLTSINQRQLEEDMLISNMLVRDAAMRFASRAQSAGVGG
                     VICSASEIAAVRATCGQDFKIVTSGIRPTWYGSNDQKRINTPYEAIKRGADYVVIGRP
                     ITRAPHPVAAAHAIAEEIESALTGFEI"
     gene            15496..16014
                     /locus_tag="PLM74_00695"
     CDS             15496..16014
                     /locus_tag="PLM74_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2148 domain-containing protein"
                     /protein_id="HON41635.1"
                     /translation="MESTLRLVAELMELSAITAPKAGGKDYIVTKVIAGDELNTLADA
                     MVQYGQQSGKRNFDRDAAGVKAAGAVLLVGIKDARPLSLNCGACGYAKCADLPEPQEG
                     PEFTGPYCAWRFIDLGIAVSSAVKTASIHNVDNRIMYRIGPVARKIGLIDANVALGVP
                     LSATGKNIYFDR"
     gene            complement(16197..17039)
                     /gene="sfsA"
                     /locus_tag="PLM74_00700"
     CDS             complement(16197..17039)
                     /gene="sfsA"
                     /locus_tag="PLM74_00700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HON41636.1"
                     /translation="MEMEHADVPIYHRTGSLIRATLIVRPNRFLAIARSDSDIRGACT
                     DEAEDEAELGVVRAHIADPGRLEELLFPGAIVYLSRAQRDSMRTPRKTEYDLVLVEHN
                     GILVSVDSRVPNELIYSALKSGFFEELREYRRITRESMYGSSRFDFRLSTGNADESNA
                     VPAAKAPDCFVEVKSVTLIQGNAALFPDAPTARGARHMRELATAVSQGFRAMVIFVIQ
                     RKDAEYFAPNAIMDPEFTEALRTAAGAGVEVKAVKCNIAKSGAIMLNKAVPVILYNSF
                     PHPE"
     gene            complement(17068..18684)
                     /locus_tag="PLM74_00705"
     CDS             complement(17068..18684)
                     /locus_tag="PLM74_00705"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HON41637.1"
                     /translation="MSPLIARLRGLRRLRRLCVRRAPYALCVLCFVSALLFAVLIGAL
                     ADAEHGETRLVLLPEARAIVDANRLPRLEVSENQLERFRELLYHYSDLSDETADLLRL
                     PARIYISNSDADSSPDGWEVSPEHAAEEVELLFGALRLGYAGYQFFGGDAAFCAAKDA
                     ILREIAELASYYDTIPNWRYSDTIARYLGFVQDSHLVFGSRSLCEHMRFRFCPHCEFT
                     LSADGSYHDLDGRRVVAVNNEAPSEYLKPSISADGDIVYILGVVTSERHQATLLELEF
                     ESGIAEMAWLMPADGVQHSNEKAYELIEFEGLPMAICRTFRTSPETAEDLSAFVRDAG
                     ILEQKDLILLDLRSNPGGAIQYGQEFCRRIATTDTGPFLMTASLLTDTALALQMNQQI
                     EANSSTDWLIRIRKERDNLMDPDVPGWNISVSAGRYVQDTPVILVLIDGKTASAAEAL
                     IRCLRSMHNVVLIGTNTGGALLTGNAGLLRLPYSGIDVRIPTMIWMDEQLRNLDGVGY
                     YPDFWVHPDAAENAAAFVRKYVVKQPVSGH"
BASE COUNT         4218 a         5861 c         5003 g         3765 t
ORIGIN      
        1 cgccggcgtt ctgcatctag ccgtcaactc ctactctctg cacaacctgg gaaacgtggt
       61 cgagcgcctt ttcggcacgc cccgattcgc tctgatctac ttgataggag gagtatgggg
      121 ttcgctggcc agcacattca tgtccgaccc gagagtgctc ggagtgggcg cctccggcgc
      181 catattcggc ctcgccggct gcctgttcta cttcggactc cgctatccca atcatttccg
      241 ggtattggca ggctggaggt tcatcgcaat cgtcctgatc aacctgttaa tcgggttctc
      301 cagttcacac attgacggat atgcccatat gggcggcctt gtgggtggtt tcgtcgcagc
      361 cctcgcactg ggtttcccga ccgagcgtac atcacatgtc cggtccacag cccgcatatg
      421 cctcgccatc gctaccctag gcgccatcgt cctggcagtc aggcccacgc ccgcatccat
      481 attcgttccg atatctatgt ccaatccggg gtctaaatca gtatcggcag ccatactcgc
      541 ttgcggcggt gtacaaatcc gctggtccgc tcagccgcat caggcaagga gccatctgct
      601 gggctccaag gctgcaatgc ccgccgacac aaaatgacga atggaaagtc ggaaaagaca
      661 cgcgcacacg aaagcgccaa tccagcagta taccgtcggc agacgagaga acaaggatgc
      721 gaactgcagg caacttgtct gatagacgac aggtttcgcg tcgaacctgt cggcccgacg
      781 tcaggtttga cgagagttac gggttgctcg gcctcttacc tcgatcggca tcgaggccac
      841 acatcgcatc acccgggttt agctgcaggt ttgctgaacg tccttcaagc tgcctgccgc
      901 gtgacgccca actctcgctc cggttcgtag gttctgtgca gaacctgtcg gccacacgac
      961 aagttgggcg cagaacctgt cggccacacg ctatggacta cccgtatcca tgggtatttc
     1021 tgggcgtgac gttctcctgt ggtgggctct ggcggggttc tggggcatcc tgtccgtttc
     1081 tgaacagcct ttccgggcaa cctggtcaga tcctacctgg ttttgcaggc gctagttgtc
     1141 tgaattcgcg ccgactgggg ctcgaccttt ttctgtcatc acgtcgccgc tgaatccgca
     1201 ggcagaggcg ttttacatca tctggtagac tgcctctgtc cctcgagtat gcccgagaat
     1261 gcctcagaca gccgcagact ttccatgaaa caccaaaaac tcccggggct tgcctgatcc
     1321 cggtcaatac cgggcatttt ctgatcaggt tggcgtacaa accgggcagt attgttacag
     1381 gtgagcgaac tccctgctaa acgcgggttc gcctaaaggc ctgctcagcg ggctttcacc
     1441 gacaactgcg catgttcaag cgcaggcaag ctcccggcgc ggtgtgttcg gctaataagc
     1501 aaggctcgcg tcatttccag cgaggtaggt aatacatcga gcccacacca caggtagggc
     1561 gcagatcctg tcggccacac aacaggctcg agccccggcc tcagaaaccg acagccccaa
     1621 ggccgcaata gccccgcggt cgtcatctac gatccctaca cgcacccccc gctgcgtagc
     1681 tgcggacgtg cggacgtgga catgcgaacg tgcgcatgca cgcgaacgcg gacatgcggg
     1741 ggtgcgtgta cgttctgtgg gcaatagcaa ttggaaaagc gggtagacat cgctaggcgg
     1801 gatagtgagc ggggaaatgg catcctctcg gatcatttcg gatcattacg gtgggttgtt
     1861 ggctgagagg gtgatgctca cctcgccggt atggctattt agctggtctt gacgtcatga
     1921 ccgtcagaca aaacggctgg agctggcata tcgtcagttt ctgctattag tttccgagat
     1981 acccgcaact tttgagtaca tcgatttcaa agggccacct gaccgcctcc aaatcacgaa
     2041 aagctggggg tatccgacgg gtttgggcag tgaagcaggc gggcgggccg acataatatc
     2101 ccacatcatg gggtcatatg gtaacggcgc gcaagtaatg ggacgtcgtg ccatcgattc
     2161 gccaacaaat cggcggcata cggctcccag ggctcgtttt gcggggtcga ttcgcccccc
     2221 ggagccctct gcccaaatcg atgtactcag aagttgcgtt tttctccgaa agtattgtca
     2281 aaatctgacc gaatctctgg gcggataccc ccgggggtat atcactgagc cacagcagtt
     2341 gtcttcccgt ccctcagcac gcccccggat gtatccccgg atgtgtccct ggccctggcc
     2401 ctattcctgc acctacacgc aggtctatcc ctgtacccgg tgcgattcca actgcctccg
     2461 ctgtggccct ggcgagacca cacgcctgaa tgcaggggat ctgcgaccct gaacgccaca
     2521 attcctacac gcacccccgc tgcgtagctg cggacgtgcg gacgtcgatt gacacgaccc
     2581 ccagggcggt agtagtatgt gggtgtgcac acttttgtga acatatccgc tcggcaggcg
     2641 acgacctcgt attcgcgtct atgcagtggc tacaggcagt gtccatatgc agcagctata
     2701 tgaagcagct acgtgcggca gctattggca gtggctatca ggagggatct acgtgggcgt
     2761 tttcgacaaa cgcatcgaca cttttctcgc tgaatacaat gacctccgcg aaaaggacct
     2821 ctactactac cttcagcagg tagaccaact tgacggcgct catgtctgga ttggcggacg
     2881 gcgcatggtc atgttctcct catacagcta cctcggcctg ctcaagcacc caaaggtgca
     2941 ggagcgcgcc cgcgaggccc tggaccgttt cggcaccggc acccacggcg tcagaatact
     3001 ggcaggcacg acagcagctc acgtcgagtg cgagcagcgc attgccagtt tcctcggaac
     3061 tgaggacgcc atcgcatata gctctggcta tgttgccaac ctgtctacca tatccacatt
     3121 actcggccgc cacgacgtag tcatcaccga caaactcagc catgccagca tcatcgacgg
     3181 ttgcatgctg tcaagcgctg agtttcaacg gttcaagcac aacgacctcg acgacctcaa
     3241 gcgcctgctc gaggcagacg ccgacaggtt cgaaggcaag cttgtgatcg tcgatgcggt
     3301 atacagcatg gacggcgatg tctgtcccct gcccgagctt gtggcacttt gccgcgagta
     3361 cggtgcatgg ctgatggtag acgaagctca ctccctcgga gtcctgggca agacggggca
     3421 cggaatcatc gagtacttcg gcatgtcgcc gcaagacgta gagatgctca gcggatcgct
     3481 atccaagacc cttcccgcag tcgggggttt cgtggccggc aggcacgacc tcatccagtt
     3541 cctgcgccac acagcccgcg gcttcgtgtt ctcagccgcc ctcccgcccg ctgcggtagc
     3601 ggccatcacc gcctccctcg atgtcattga ggaggatccg tcgctcatcg ccgctgtgcg
     3661 ccgcaacatc cagcggttcg tgggcgggct gaacgagatg ggctacgaca cactgaacac
     3721 taagagctgt gtgattccga taatcatagg caagcccgaa cccacactcg agctgacgtc
     3781 caggctgcac agggacggaa tgttcgtatc gcccattcta cacccggcgg tgccgatgaa
     3841 cacctgccgt ctgcgtgcca acgtaacagc agggcacacc gacgaggaca tcgacttcgc
     3901 ccttgatcta cttcgcaggc atggaaagga catggggatt atcaaatgaa ggccgttcag
     3961 ttcgagggga ccgttcccag gtatgtgtat tcaaccgtag caggcgccat aaaccgcaac
     4021 gcatattacg accgattctc caacatcgta ctgagggacg ttcgtccccc cgaactaccc
     4081 acgccagaat gggttagaat caggaccaag tatgccggat tctgtggcag cgacaagaac
     4141 ctggtgcttc tgcacgattc gccgtccaca tccccgtacg cgtctttccc gttcaccatt
     4201 ggccacgaga actgcggtta cttgtccgaa atgggcgctg tggcagccga acgcctgacc
     4261 gtcggcatcg gcgcccgcgt cctagtcgat ccgctgctct cgtgcgttcc cagaggcatc
     4321 agtcccgtct gccccagctg cgcgcggggc gattactcac tctgcaccaa cttcaccgaa
     4381 gggtcggtct caccaggcat gtccatcggg acatgccgcg acactggagg cggctggagt
     4441 tcagagttcg tggcccatta ctcccagctg atcccattgc ccgacagcat cagcttcgaa
     4501 gacgcagtgc tcgttgacgc gttctgctcg tcgttgcacc ctgtgatgcg caacttccca
     4561 tctgatgact ccacagtgct catcatcggc tgcggagtaa tcggtctctg cgccgtcgct
     4621 gccatacgtg cactcggcgg taaggcccgg attgtggtcc tggccaagca cgagttccag
     4681 ggcgaagcag cgacacggct cggcgccgac gccgtagtct atatggcgaa ctcccgagac
     4741 tactacgccg aactggctga catcctcaga acgaagttgc tcaagcccat gctgggcaag
     4801 agagtcgtag tcggaggggc tcataccgtg tttgattgcg ttggcagttc taccagcatc
     4861 gacgatgcat tgcggttcac gatgcctcat ggcacgatgg tcctggttgg cttggccgcc
     4921 ttccccaaag gcgttgactg gacccctatc tggctcaagg aagtccaggt gcgcgggtcg
     4981 ttctggtgtt caaccgagca gttcgagggc aaagccatgc gcacctacga gatcgcagtg
     5041 gaactgctac gttccggcag gctcagccta tcgcacctgc ttacccataa gttcagccta
     5101 gaagagtaca aacaggccat tgaagccaac ctccacctga gcaagtcccg gctgatcaag
     5161 tcggcgttcg agtttgactg acgcccgacc gaagtggcac atgcgtctgg gctgcaacga
     5221 tggtatactt gatatagcag cacagcagca cagcagcaca gcagcacggt ggcaacatgg
     5281 cagcaccgat gaacgactag acgacgcatg ccggcaggca accgtaatgg cggcctggcc
     5341 agatggaggt agcaacagct ttggcacgag tcgggatcac ggacacgacc tttcgcgatg
     5401 ctcaccagtc gcttttcgcc acgcggatgc gcactcacca catgctcgag atagcggcat
     5461 acctcgacaa agtagggttt cactcgatgg aagtgcgggg cggcgcaacc ttcgacactg
     5521 ccctcaggta cctcaatgag gacccatggg agcgccttcg cctgatacgc gagacggttg
     5581 tgaacacgcc tcttcagatg ttgctgcgcg ggcagaacct ggtgggctat cggccttacg
     5641 ccgatgatgt agttgatgcc tttgtcggca agatggtgga actcgggatc agcatcataa
     5701 gggtgttcga tgcgctcaac gatatccgaa acatcgaaag atcagcggct tcagtgaagc
     5761 gcttcggagc tcaccttcag gggacgatct catataccgt cagtcccgtt cacgccctgg
     5821 atggctatct cgactacgcg cgccggctta aggatgtggg cgcagattcc atatgcgtca
     5881 aagacatggc tgggatcttg acaccatatg tagcttacga cctggtgcgc cgactagttg
     5941 acgaggtcgg cctccccgtc cacctgcaca gccactatac ctccggcatg gcgtcaatga
     6001 cctaccttaa ggcgattgaa gcaggcgcag cggtcgtgga ctgcgccacc tccacattgg
     6061 caatgggcac atcgcaacct gcgtctgaat cgctgatcgt ggctcttgcg ggcaccgggt
     6121 acgacaccgg ccttgatgcg aggctcatca ggcccgctgc gtctatcctg aggcgtatca
     6181 ttgacgacac ccctgagtac cgcgacctca tcggcaatat gcaggtcaac acagacgtgc
     6241 tcgtctacca aatccccggc ggcatgatat cgaaccttcg cgcccagctc agacaagtac
     6301 aagccgatag ccggatcgaa gagatcctag ctgaagtccc gagagtcagg gcggagatgg
     6361 gttaccctcc tctcgtcacg cccataagcc agatcgtcgg cgcccaggcc actttcaaca
     6421 tcatccaggg gcagcgctac aagagcatat ccaatgaggt ccgtgcctac gtcaggggtg
     6481 agtacgggcg tccggccggc gagatcgacc cctcgctcaa gcgcatggtg ctcggagacg
     6541 agaaacccgt gatatccagg cccggagacg gcctcacacc ccagatgccc actgctcgcg
     6601 aagcggtagc accatatggc ggttctgaag aagacgcgat atcgtatgcg ttattcccta
     6661 aggagacaga gaattacctc gctcacagag caagtcaccc gcgatcatag ataccggcag
     6721 ttgcagggtc gcatgagatt ccactcatca ccatgcgctt aatcaaacgc gcagagcccg
     6781 cagggggtaa gctaccgtca cctaggtcga ggacggccgt ggcaagtgcg ctgagcacat
     6841 accggattac cgtgtcggta tcgatattga gtatgcgtcc ttcagctttg cctctggtga
     6901 tgatcggccc aactagcttg gcaaagcggt cgcggtattc agctacttca gtcagccatt
     6961 ctcgacccga agcgagcact tctctgaaga acaatcgcat gaagtcaatg tgtcgtctgc
     7021 cgtgttcaag cagcacatca agcacacgtg agagctgctc tacgggatca tcgaccttct
     7081 cgatactccg ctcggcatca accagaagac gctccagccc gtccgatatc atcgccgcga
     7141 aaagctctcg cttgccgccg aaatgatagt atattgtgcc ttttgccacg cccaggtgcg
     7201 ccgctatctc atccaccgag gtcgactcaa aacccttctg cgcaaacacc cgggctgcac
     7261 cgcggcatat ctccctcttc ctgcccgtcc tgttcatccc aagccactgc tcctctgccc
     7321 cagctatttc actcatatgg cggcctttat cttcatcagg aaccggacct cgcctatcgc
     7381 gccctccggt ttgcctatga agctgtcgta acctgccaga cgatctgcca tagccgccaa
     7441 caccgcctgc cccttttccg cctcagccag acccttgctg agttcatccg ccatccggga
     7501 cgtgccttcc ttacgcatgc gcgctaaccc ccctgcaagt tgcctggccc ccgatgcaag
     7561 ctcctcgcac ccgccccgga tcgtgcccac accgcttgat gccgccgtca gcccctcaac
     7621 ggtggtgctc atccctggaa gttcggcacc ctcaacgctc ccgccctcag ccagcagcac
     7681 agtcccgcgc ttcaggtcat taagccccgc gtctatctgc ctcagtcctt cgcacaggac
     7741 acccatccca ggtatcgtat tgccttcaac ttcgccgccg taaaccaccg cattgagcgc
     7801 agccacagcc tgcatggcct gggcgatcat ctcgtcggat tgggacaggt atcctgagac
     7861 caggctagtg ccgagcttca gccttgctgt gttcgaggcc agcccacggg cggtttcagc
     7921 cagctgtgga agtgaaggca cctgcgaaaa ccccactctc ccacctgcac tgagcatgtt
     7981 cacggccgca tcctgcttct tcagcgcagc ggacagagca gcgtattctg aagacttggc
     8041 aatggacggg ttggcccgcg ccaatgcctc agctgcagcc atagcgtccg agccggccga
     8101 cttagccgac gcgactgtct gcgcctgcga cgcaagcgcc ctggacaggg tttgggccgc
     8161 tgcgtcagag tccgtcacgc cagacacaac gcgctcagcg aaccctgcat taccgcccaa
     8221 cagatccgca ggcagcatcg acacgactga gcccacaccc tccgcaatct gcgtcagtat
     8281 cgcgaggtcg gcctccgcta gctgcgcaag ctccagcggc gcctccagaa cctgccccgc
     8341 gagcccgtca ttaatcgttc tggctatcgt atagtgcgca tctgcgagac gtgccaggtc
     8401 gcgcagcccg tcttccagcg cggcaatccc cttctccagc tcgtgctggc cctccgcgag
     8461 ccgcgacgcg ccgttttcgg cctcccgaac agccgcgtcc agcgcagtcg cgccggcggc
     8521 gaactcccgg agttgatcga ctatgtccaa ctgggacacc aggggcagcg caggtattat
     8581 cgacacatcg aaccctgccg gactcatgtc gtctccctcc aggatcaggg aggccgatgc
     8641 gtcaggtgca gggaataaca tccagttgac cttgaggtcc tttcccgcca gcactgtagt
     8701 agctcccggg gcatcgatgc acctataaga cgggagcggc acatccgagc tgatctgcac
     8761 gatcatcggg acgcacacat ccgcagagaa agcaacggat gctcccggtg acccgaatgc
     8821 gccgtgttgc agtttctggc tgcggattgt ggtgttatgt agactgagac gtatctcaag
     8881 cctgccggag ctaccgacca cctgctcagg ggggacctcc gtcccgttca ggatgtacgc
     8941 ggccttgatt gacaccggca gtggtcggct ggacctgcct gagtagttaa tatcggtcgt
     9001 ctgtgacggg tcagaccccc aagtaagccc ggtctcgctc acttgcggca cttcagggcc
     9061 acgcaaattc acaacatcgg tgagatcacc ggggtcgaat atgctggtcg gcccgccctc
     9121 gggcgaacgg atccagtcca caacagtagt cgacatcacc tgcccgtctg gcgcaagcac
     9181 tgcaaacacc gtctcataat cgcgaatccg cgaaagagtc cccgatccag cctccccagt
     9241 cgcggttgag gatcgagacc cgactgtggc ctcagccagg gccgcagact gccgcccaat
     9301 cgccggcctt ccagcagcaa tcaggacgag cagcgcgcac aagcagagga ccgatctctg
     9361 tctggtcttc ttcatccgaa acaccccaat cccgaaaaca tgacactgcg aggttcgcca
     9421 acagagcatc aggcatctgt catccagcat caggcatcag gcccaccgtc tgtcccttca
     9481 ggccagcccg cagaggtctt cgccagcacc ttctgcacca caagcagaat ggccggcagc
     9541 agcaccgtgg ctacaattgt gctcacaatc gcgcccctgg cgagcagcac agcaaggtcc
     9601 tttaccgccc tcaggctggt tgacgcatac actcccatga tggctgcgaa catagcacct
     9661 ccactgcaca agatggccgg gccactctct tccacagcta gacgcatggc gtgcgtcggg
     9721 ctgcaggtgc tcaactttcc cctgaaggtg ctggccatga gtattgcata atccacagtg
     9781 gctcccagct ggatggcgtt tatggcgagg cccgagaaga agaagagcgg agtgtgagtg
     9841 aaatacgata tcgactggtt gagccagatg gcgcactgga tagccgccac cagcacaaca
     9901 ggcgcggtca aggagcgaaa cgctatggcg ataatgatga agatggccgc gacggataca
     9961 atgttaacca cggatatgtc gtgtgaggtg agcctcatta ggtcgcttga gatgaccggc
    10021 ccaccggtaa ggtacaccgt ggcatccgac tcgtcgagca cgcgcggcat catctcccgg
    10081 atgtcggcaa tcaggctatt cgtagcctcg tcagatgcct tgctatcgat actcactatg
    10141 gctgcgctgt attcgcccgc cacatacctt gatgcaacgc ccttcggcac gaaatcgatg
    10201 ggcacagccg gatcgaccgc atcagccagg cagctggcac caacgactcc gcggataccg
    10261 cgaagagctg tggttattcg ctgaagtcgc cagccgggca ctccccctgc aacaacatac
    10321 atctctcgta gcccgctgac cacatcgccg aactcctcca tggccgcaat cgacggcata
    10381 tcgcggggaa acgtgctttc aatgtcgtag ctcaggctca tccgcgagga tccgacgtat
    10441 cccaacacga gcaccaggag agccatggca aacacaggaa atcgccttcg cactacccat
    10501 gaggccagac cgcgaaaact tggaagcagc acaggatggg tcagtctgtc gatgacattc
    10561 tgaagcgcta gcaccaggct gggcagtatc accaacgtac acactaccga cagcagcaca
    10621 cctctggcca tcaggagccc catatccgca cccagtcgca cacgcatcaa cgccagagct
    10681 gcaaaacctg cagccgtagt gagcgcgctc gacagaattg cgttggcagt cttgacgatc
    10741 gcccgctcca ccgctgcttc cgcgtccgcg ccgtctcggc gagtgccgcc cgtgctcgcg
    10801 cccacaacga tctgattgcg ctcagcgtga aaactgtgca tcaggaagat gcagtagtcc
    10861 atggtcacac ccagctgcat ggcggcagct accgagctgg tcaagtaaga caccttctgg
    10921 cccagtgcgt atgatatgga catgttgtag gcataggcgg cgccaatggt gatcatgaat
    10981 atgaagggca tagctgctga aggcaatgtc acaatcagca ccaggaagat cagcactgca
    11041 gcggtccgta tcatcttggg catctggctc atgcccagtt ccttcatttc gatcccgctg
    11101 actacctcgc ccacaaggta ctggtcctcg ccgagcagac gcctcatctg gtctatcgcc
    11161 cgcacagttc ggggcgaaga gctgtcctcg gccagactga ccagaaggaa tgtgatatct
    11221 ccctcatctc ccctgtagaa ctgcgacacc agttcctcgc ccaggtagtc gtgggggatg
    11281 gttggatcgg ccaagtccga cagccagaag actgacgata ccccgtctat cttcgaaagc
    11341 ctgtccttaa tggaaaggac agcccagtca ggctggcctt tgaccaccac cgccgctcgc
    11401 tctgcatacc ggaactcgcg atccagcact tcatacccgc gcacagactc cagattcctg
    11461 ggcaggtact tcagcaggtc gtactcgatc ggcacgttca atgtgccgta cactgcaggc
    11521 acgagcaaag cagccgcagc aatcagaatt gccgcccggt gtcgcaccgt gaatcgagga
    11581 atcgaattga cgaggtccag aagccgaaca ccccagcgca agtcgcagcc ccgccctccg
    11641 caggttctgt aatgactaat ctcgggccga ccggttttgt actgaccggt cagtacagcc
    11701 caattctagc cagcggagcc tgttctgtca actgagggac ccggagtctc ggtgacccaa
    11761 aacctcggcc acgcaaaacc tcagcaactc agacctcagt aacccaaacg cttaccgggc
    11821 ggacgcctcc ttcgccctcc tcacgaccgc cacagcagtg cctgccagca gaagcaccga
    11881 acccacccag agtagactgg cgaagggttt cacgcttatc tccagcgtca cggattgctg
    11941 gccgctctgg tttccttgca catcgtcccc aggcagcaac ttgcctatgc gaagcacagc
    12001 cataccggca cctgcatcaa tcgacgtaag cctgaacgcc acactccgag tccctgcagc
    12061 atccccggga ttcgcagaat ccgcagcatc cagatcaatg agagcatctg tctgcatttg
    12121 cccacccgct gcaaaaccca gcactggagt tacagtcccc aattcacgac tggatgaaat
    12181 gtccaacacc tcgagagtag ccccaacagc catcacgttt tcgcccaggt gctggctcat
    12241 atcaaaccca acaaacttca cagccaggcc gtcaacctca acctgctcgc ccttgtgcaa
    12301 cctgacctcc ttcgcattgt ctgcccatga aggctcccga gatgcatcct cctgaagcgg
    12361 cgagatgtag aggtcgctgg cgatcgtact tactatggcc ggatggcgca gcatcccctg
    12421 attagtaggg cgcatatatg gctttgcggt aatcgcagtg ccgcttccgg gtttggacac
    12481 ctccagattc caggcgacag acggccggtg actgcccgca gcctcgaatt cgtcgcgcga
    12541 tacatacgcg accctccagc caaacaccgt aagctcctcg cccgcctgca agctgatagt
    12601 ttcagtccga ttgtaggcag acgtggcaat gactccgata agaatcagcg ctatgcccag
    12661 gtgagcaacg agaccaccca tcacccgcaa actcatacgc gcccgtatgc gcttgaccag
    12721 cacaactaag ttgcctgcta aagccatgaa tgctgcaaac aggaagagga cgaaccacac
    12781 gccggtggct ccgagcagca ccgcaaccac ggtgagcaca atggccgcaa cagccgggat
    12841 cagcatcgcc ttaccgacct gcgcgagtgt cgaagcgcca taggccaaat gggggcaaac
    12901 cgcaactgtc agcattagca tgaatcccag cggggcgttg gtcatattat agaacgcctg
    12961 tcctacagaa gacggattcc ccgaaaagct tgtgagcagc ggggtgctgg tcccgagcga
    13021 gacaattgct gctgatgcca caaacagcag agccgtgagg aaaaaggcga aatcccgcga
    13081 cacggtctgg tcatatggtt ctctgtaatt gcgctttgag gtcatactcc gccagcgcca
    13141 cacaagcatc cagagtgaca atgttgcgaa gagcacacac gcggcaacca ggtaggacga
    13201 cagccccagg ctggcaaagg agtggactga gaagtccgag agcactccgc tacgagtgag
    13261 gaatgttgcg taaacgatga gcagaaacga gactatggcc aacacaatgt tagtcctcac
    13321 catcttgccc cgctcacgct gcactatgag ggtgtgcatc aaagccgccc cagcaagcca
    13381 cggaaccagc gacgcgtttt ccaccgggtc ccaaccccag tatcctcccc atcctagcgt
    13441 ggcgtaggcc cacttagctc caatgatgat gccggcgccc agaaagagcc aggccgcaac
    13501 cgtccaggcc aatgcgggtt tcgcccattc cgagtactcg tcccgcgcaa gtgcactgat
    13561 ggcatatgca aatggaaccg cgtatagcgc atacccaatg aagactatgg gggggtgtat
    13621 caccatccac ggatcttgca gcagagggtt caggccagcg ccgtctgcag gcactgcagg
    13681 cagcaagcga aatggggtgg ccctgagcag caggaccatc agtaccacat gcactagcac
    13741 gtagaaccgc atcacagagg cctctgcccc tgcggacact actgcatcag gcactgccga
    13801 tgatgagtac gacgaagccc ctgtgtttgc tgggattcgg gcgcctgcag cggtgtgact
    13861 ggcgctggcg gccagtatca gtccgatggc tcccgagatc gcagcccaca gcaggaacgt
    13921 tccttcctga ccggcccaga acccggatac gaggtagaag gcagataatc ctcttgaaga
    13981 gtagctggca acataggcaa gacggaaatc gtgtttgaga aatgccgaga acagtgccgc
    14041 tgaggccgcc agaagcagta tgaactcaac tgcaaacaag ccgcgtgcca accgcgatcg
    14101 cctctcgcgc atagccgctg tgcgcgcgct gcttgacata aagtagaaca caagagagaa
    14161 acatgatgca acaaaagcgc cgattagcgc ataattccag ctgctatagg tagtcaagta
    14221 acgcttcctc ctctcaatac cacgtctgcc gctgattata tcgttatcgc ggaccagtgt
    14281 aaacgtgaag gcaacccgca acggcagact gcaacatgca atgattgaca gaagcatcag
    14341 ctttcgatat catccaatcg atgagcaact acaagttcaa agcactacgt acaagcacgc
    14401 ctgatcagtc aagtcttcca cttatgaaca cggccttgca ggctcatctt cgaagatcgc
    14461 attttgtctc acacaacgat acgcgagaca caagacggcc tgagaaggag ggcagagtgc
    14521 cgctttgcga gtagttccag aatggcggca cgttgcatgt gaactcaaag gaccagatca
    14581 tactggcgct tgatgttgat accgaggaag aagcgttgtc cctcgcaaat aggctttccg
    14641 ggcacgttgg cgccttcaaa gtcagccttc gtctgttcag catagcaggg ccccaaatcg
    14701 taggcagact acaggagatg gggcaggtat tcatagatct taagcttcac gatatcccca
    14761 gcacagtagc ggctgccggt cgcgccctga cacggctggg atgcagtatg ctcaccgcgc
    14821 atgccgccgg gggaacggta atgttgagag aattggccaa tgctgtctcc gaagagtcgc
    14881 gcaaaaccag caaaccggcc ccaacggttc ttgcggtgac ggtgctgacc agcataaacc
    14941 agcgtcaact tgaggaagac atgctgatta gcaacatgct cgtgcgtgat gcggccatgc
    15001 gcttcgctag cagagcccag agtgccgggg ttggcggggt gatatgctcg gccagcgaaa
    15061 tagctgctgt gcgtgcaacc tgcgggcagg atttcaaaat agtgacctcc ggaatccgcc
    15121 ctacatggta tggttccaac gatcaaaaac gaataaacac cccctacgaa gcgatcaagc
    15181 gcggcgcgga ctatgtcgta atcgggcgcc ccatcacacg agcacctcac ccagttgcgg
    15241 ccgcgcatgc catcgcagag gagatagaat ctgcgctcac ggggtttgag atctgataca
    15301 gcctggtgta tccgcgtaac atctgatgcg acctggctcc atccgtgtaa taactgatgg
    15361 gacctggcgc attcaagtaa cagttctgag cccaatcgca gcagcaaaat gtggagggag
    15421 gaaggacttg ccggggcaat gcagtgtgct gaggctccag agatgctgca tttgactgcg
    15481 ggcagcccaa atgtgatgga atcaacactc agactagtcg cagagcttat ggagctatcg
    15541 gctatcaccg ctcccaaagc gggaggcaaa gactacatcg tcacgaaggt catagcagga
    15601 gatgaactca acacccttgc ggatgcgatg gtgcaatatg gtcagcagag cggcaagcgc
    15661 aatttcgacc gcgacgcggc tggtgtaaaa gcagctggag ctgtgctttt agtcgggatc
    15721 aaggacgcca ggcccttgtc gctcaactgc ggcgcatgtg gttatgccaa atgcgccgac
    15781 ctaccagagc cgcaggaggg gcctgagttc actgggcctt actgtgcctg gaggtttata
    15841 gatcttggca tcgcggtatc gtcagctgta aaaacggcat ccatccacaa cgtagacaac
    15901 cgaatcatgt accgcattgg ccccgttgcc cgcaaaatcg gccttataga cgcgaatgta
    15961 gcactcggag tgccactgtc ggcgaccggg aaaaacatat acttcgatcg ctaatcacaa
    16021 acacacaaac acagcacagc cagccccgac ctggaagcag attcctgcaa taccgggatc
    16081 caatcaggaa ccggattcca gtttgcgtcc ggaatccggt tccaactcat catgttgcgc
    16141 gcacagcaaa agcagtcata gcatatgcag atcatcgcag atcaggctgt ttggggctat
    16201 tcggggtgcg gaaaggagtt gtacaggatc accggaacag ctttgttgag cattatcgcc
    16261 cctgatttcg caatattgca tttcactgct tttacctcca ccccagctcc agccgccgtg
    16321 cgtaatgcct ctgtaaactc ggggtccatt attgcattcg gcgcgaagta ttcagcatcc
    16381 ttacgctgta tcacgaatat caccatcgcg cggaagccct gcgaaaccgc tgttgcgagc
    16441 tcccgcatgt gccttgctcc gcgtgcagta ggcgcatcgg ggaatagcgc ggcattaccc
    16501 tgtatgagtg tgacagattt gacttccacg aagcagtccg gagcttttgc agcaggtacc
    16561 gcattcgatt catctgcgtt tccagtggac aaacgaaagt caaacctgct actaccatac
    16621 attgactccc tcgttattcg tctatattca cgtaactctt caaagaaccc tgacttcagg
    16681 gctgagtata tcaactcgtt gggcactctt gaatccacgg agaccaaaat gccattatgc
    16741 tccaccagaa ccaagtcgta ttcggtcttc ctcggcgtac gcatactgtc gcgctgcgct
    16801 ctactcagat acacaattgc acctggaaac agcagttcct ccagtctccc gggatcagct
    16861 atgtgagccc tgactacacc cagttcagcc tcatcttcag cctcatctgt gcaggccccc
    16921 cggatgtccg aatccgaacg tgcaatcgcc aaaaaccggt tgggcctgac aattaatgtt
    16981 gcccgaatta ggctgccagt ccgatgataa atcggcacat ctgcgtgttc catctccatc
    17041 tactgctaac acacctcttg taatgtctta gtgccccgaa acaggctgct tcaccacgta
    17101 tttgcgcacg aaagccgccg cgttttccgc tgcgtcaggg tgcacccaga aatccgggta
    17161 atacccaacc ccatccagat tacgcaactg ctcatccatc catatcatcg taggtatcct
    17221 tacatcaatc ccagagtatg gcaatctgag caaccctgca ttgcctgtaa gcagcgcacc
    17281 acctgtattc gtgcctatca gcaccacgtt atgcatgctg cgcaaacatc gaatcagtgc
    17341 ttcagccgcg cttgccgttt tgccatctat cagtaccagt atgaccggcg tatcctgcac
    17401 gtatctgcct gcagagacgg atatgttcca tccgggcaca tcagggtcca tcaggttatc
    17461 gcgttccttc ctgattctaa taagccagtc agtgctgcta ttagcctcta tctgctggtt
    17521 catctgcagt gccaaggcgg tatcagtcaa taagctggca gtcataagga acggacccgt
    17581 atccgttgtc gcaatgcgcc tgcagaactc ctgtccatac tggattgcgc ctcccggatt
    17641 cgaccgcaga tcaagcagta tgagatcctt ctgctccagg attcctgcat ctcgaacgaa
    17701 tgcgctcagg tcttcggcgg tttcgggcga tgttctgaaa gtcctgcata tcgccatcgg
    17761 cagtccttcg aactcgatca gctcatatgc cttctcattg ctatgctgca ctccatcagc
    17821 aggcatcagc caggccatct cggctatccc gctctcaaat tccagctcaa gcaatgtagc
    17881 ctgatgccgc tccgaggtta ccacgccaag tatgtacaca atatctccat ccgcactaat
    17941 ggacggtttc aggtattcag acggcgcttc gttattcaca gccacaacgc gtcgtccatc
    18001 caagtcatgg tacgatccgt cagctgacag tgtgaactcg caatgcgggc agaatcgaaa
    18061 acgcatatgc tcacatagcg acctgctgcc gaaaaccagg tgcgagtcct gaacgaaacc
    18121 caaatacctc gctattgtgt ctgagtaccg ccaattgggg atcgtatcgt aatatgaggc
    18181 taattcggcg atctcgcgca ggattgcatc cttagcagcg caaaatgctg catccccgcc
    18241 gaagaactgg tacccggcat agccgagcct gagcgcgcca aacagcaact ccacctcttc
    18301 ggcagcgtgc tccgggctta cctcccagcc gtctggacta ctatcagcgt ctgaattcga
    18361 tatgtaaatc cttgcaggca ggcgcagtaa gtcggctgtc tcgtctgata gatccgagta
    18421 atgatagagc aactcgcgga agcgctccag ctgattctcg gagacctcaa gtctgggcag
    18481 cctattcgcg tccacaatgg ctctggcttc aggcagaagc accaaacgcg tctcgccatg
    18541 ctctgcatca gcgagcgctc cgatcagaac tgcaaatagc agtgcagaca caaaacacag
    18601 aacgcacaaa gcatacggag cgcgtcgcac gcacagtctg cgtaatcggc gcaatccgcg
    18661 caatcgggct ataaggggac tcatatcatc acctccctga cggcatagcg ctcatctcga
    18721 gctaaggctc ataagtgctg ctctccagtt catccgccca ttccgccatc cgcgcaatcc
    18781 gcgcctgaag cagttcctct gtaaggccgg caaacgcccc gcagcataat ccctcacccc
    18841 gccaatc
//
LOCUS       DAOTAC010000008        18057 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_2568_length_18057_cov_4.720864, whole genome shotgun sequence.
ACCESSION   DAOTAC010000008 DAOTAC010000000
VERSION     DAOTAC010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 18057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18057
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2568_length_18057_cov_4.720864"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="PLM74_00710"
     CDS             complement(<1..541)
                     /locus_tag="PLM74_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41638.1"
                     /translation="MRMTDLATSPNVGRGPEEHLPRFDPGITEPIWKIRWKMNMRSLA
                     QNWEIFKESRVGMSGLIIIIAFGIFGLLWPIMISFVWDPLIYHPVVGFDPMLMHPSPP
                     MAGHLLGTDPIGRDILAQLMFSTGREFLLGVLSAVISVVVGTFIGAMAAYYGGFTDTL
                     FMRLADIVMLFPFVPLLVVI"
     gene            complement(546..1724)
                     /locus_tag="PLM74_00715"
     CDS             complement(546..1724)
                     /locus_tag="PLM74_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013933230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HON41639.1"
                     /translation="MRRYMLGRVGQLVLTLLIYMTMVYFLMQAMPGDISQVYLANPRI
                     PQAARQELKKQLGLDRPPVLQYLSYIKNFFTGNLGVSFSHYPRPVWDILIERLPRTIV
                     LFVTANCLSFYVGFVLGRIIAWQRGATVDHIATVVGITFWTAFYPLLSLILIWLFAFT
                     LHWLPLNQFIEPALWVKAPFSSNTVFGYMVLGASVIALAVFLIFWVFRRGLRRTRKSA
                     ALAWASTAGVIGLAIWIAHATGTLIYIWDIIRHMILPVCTLTLINFAGTMLLMRDSML
                     ETVQEDYVMAARAKGLPDNVVRDRYAARNALLPTVTSLVLSLASSMSGGLVTETMFSW
                     PGIGQVLFRAALGNDYPLATGALVFTGVFLLVAHFVADVLYAFLDPRISYTGQPAAEG
                     "
     gene            complement(1833..3392)
                     /locus_tag="PLM74_00720"
     CDS             complement(1833..3392)
                     /locus_tag="PLM74_00720"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HON41640.1"
                     /translation="MRIRRDLKWSDGKPITADDVAFSYMAPLALDPNKLGGNWPGNID
                     PDILARVEKVDDYSVKFFLTELPGLAQWQYGMLQAYILPKAYWGPIVDKALKAADPVK
                     ELLAHDPVGEPVAGPWVVERWEKGAFVTHTPNKYYTFKGDTNTFYTSGGFMIENKKTG
                     VKWTTGDAKGDIKLQVVEGPYVDQLRFRILQNQNAAVLSLMKGEVDFVLNSLGLQRGF
                     QEQLKKTPNITTIENSVNGFRYMGFNLARYPFGIKEFRQAVATLIDREYITQRVLQGV
                     AFAQYSVVPPGNAYWFNTDVKMWGKGLTRSQRIVEAVKLLKSAGFSWEVEPQVDVEKD
                     KVLKRGKGLIMPDGKKMPSFEFMVMTAGYDPLRYTFGLNIGTWMQEIGIPCEVVPTEF
                     NVVLTRTDDRDYDAFLLGWSLSLYPDHMHWFFHSSQAPAGGFNATQYVNPEFDKLSES
                     FLAEPDLVKARDKAFRMQQMLAEDLPYIVLFDTPLIEAYRSDRLEYPFTKVLSGIQYF
                     GGMTSTVKLKN"
     gene            complement(3432..3704)
                     /locus_tag="PLM74_00725"
     CDS             complement(3432..3704)
                     /locus_tag="PLM74_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41641.1"
                     /translation="MKNLRFGLAVLLFVAVIAGMSVMGQAAAKVYEMAIFEDPTTLNI
                     WAQLGPDASVWNAFVGYQQSYIGLYGNAAPDFVFSPSAALGMPTPF"
     gene            complement(3818..4345)
                     /locus_tag="PLM74_00730"
     CDS             complement(3818..4345)
                     /locus_tag="PLM74_00730"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HON41642.1"
                     /translation="MIRHRRVLATTLLTLAAIAVGLAFGGGEVLAQPASLLGGSAPQV
                     RATSLDGGEVVIPVRGKVMLVVFWNTKYRLSADALGAIQRLHDRHRARGLLCVGINDL
                     GEDARVISQFVSGLGATFPVVGGPAGPSAAASYRVRGVPVVYVIDRSGAVSYVKEGWD
                     KRAEDEVTKKLVEAL"
     gene            complement(4510..5556)
                     /locus_tag="PLM74_00735"
     CDS             complement(4510..5556)
                     /locus_tag="PLM74_00735"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HON41643.1"
                     /translation="MEHRIVKTQFVADLRPGMMADTVFLCADLKSRMTRNGSYFADVR
                     LVDRTGEISLKLWRYSPDATLGLEPGACVRVSNVQVEEYGGSLQLSQDTSSFGRSITL
                     CDVSQCNLADYVPATPRDVDEMIREVTAAVSGVSNRPIRCLLQSFFGDSEFMARFREW
                     PAAIRHHHAYCGGLLEHTVNVLRICRQAAEQYASVNWDVLCAGALLHDIGKVQSYDYD
                     RARAQAAMSPNGVAVDHIVLGMELVSRRIAELVSDCANKGSCASPCWTPGLTLEIAHL
                     VASHHGTLEWGSPVQPATIEACILHHADNMDAQVNKFERTIRESFASGEGEYRYSELV
                     GRTVWMPLRPESQK"
     gene            complement(5577..5987)
                     /gene="ndk"
                     /locus_tag="PLM74_00740"
     CDS             complement(5577..5987)
                     /gene="ndk"
                     /locus_tag="PLM74_00740"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HON41644.1"
                     /translation="MERTFVMIKPDGLMRGLAGKIISMFETRGFSVVRARTLVMSEAL
                     VREHYLHLADEPFFDEIKAYMMSGPVLAMVMEAPNAVEAARQILGATDPMKAAPGTVR
                     GSLATSVTYNLAHAADSREAAFREIERFFGPRVE"
     gene            complement(6004..7068)
                     /locus_tag="PLM74_00745"
     CDS             complement(6004..7068)
                     /locus_tag="PLM74_00745"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HON41645.1"
                     /translation="MQIAIILYGFGNVGREFVRLVNRKRSYLKSRHGIEPRVVSIVTR
                     KGGIRAESGIEWPVLVNLANAGTLSEQEGFVGGNPDDLDLWLPSEESGHIRVLVDATW
                     SDLATGEPALSRLKGAARRGCHLVTLNKAPLVADFDGMVQAARDARVRLKFSGATAAG
                     LPTIDTVRVSLAGAEVHSIEGVLNSTTNYILTAMSDNALSYEEALRRSQALGLAETDP
                     ERDVSGIDTACKILVLANAAMGARKRLSDVKITGITEVTPRQIAYWAVQGRTLRLIGR
                     AVRNGDDVSIEVKPTPVPYKDFLAQVTGDSKGVRFTTDMFGELTIVGGAGGPSAAASS
                     ALKDLVNLARAIDGDQGLIP"
     gene            complement(7080..8405)
                     /locus_tag="PLM74_00750"
     CDS             complement(7080..8405)
                     /locus_tag="PLM74_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019635416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HON41646.1"
                     /translation="MTRKLDYPRTFTLGLGFFAISLSWALYNAFVPVFLDNVLADTVL
                     KGTLIGFIMTFDNIAAVTLQPYFGAASDRTWNRYGRRMPYILAGMPLGALFFAVIPLF
                     RSSLASMMTALIAMNLAMAIFRAPTVALMPDITPPALRSKANGVINLMGGLGALLAFF
                     VGSRLYNVNPALPFVFAGLLMIIITLVLKWRIREPEVVEGAGEGSETVGILEALGEVR
                     RDPDRSARCMLLAIFFWFIAWNGIEAFFTLYGKDVWGISEASGALYLGFFSLALVAFA
                     IPAGFLATSIGRARTIKIGIVGLAVCLSIIGLVGSDALGRPVLVLLNKLFYAGSVEGD
                     GQLAIVGAILARASVIVPAVLALAGMFWAAININSYPMVVDMAAKSKTGAYTGLYYLF
                     SSLAAITGPPVFGFLRDLFGMRALFPFAVLCALIALMFMAGVKKGEPQV"
     gene            complement(8402..9139)
                     /locus_tag="PLM74_00755"
     CDS             complement(8402..9139)
                     /locus_tag="PLM74_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HON41647.1"
                     /translation="MPLILGHRGVPVISPENTLAGFRKAIEMGSDGIELDVHLSRDGQ
                     LVVIHDERLERTTDGSGFVGEKSFAELCELNAAAKFVHGAEVQRIPTLDQVLDAVGDA
                     CRLINIEIKSGVVLYPGIEEKLVDLVRRRGAAKRVIFSSFNHYGLVELKRLAPEMAIG
                     LLYMGALVEPWHYAARLGAEALHPIHFTVFPEFVAGAHAAGVQVNAWTCDSPADISRM
                     LASGVDAIITNDVPLAVKMREEESEQP"
     gene            complement(9246..10268)
                     /locus_tag="PLM74_00760"
     CDS             complement(9246..10268)
                     /locus_tag="PLM74_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41648.1"
                     /translation="MSMCEFLDAAIRLDPYCAEASDRFAAEFGTASGAPSWRPVLGFR
                     ASSSALEAWATAEGPPGVSCRLTYESGRPGSSENRGMATWRGVVEAPLSCIPRIIRAE
                     AVSLEEALLRAAELRVAMVEAGAAARAVDMLRKAEVLAARCDAVGAMQAYGELSLLIG
                     LPSDEIRPVVMSDDGPTHLAQLIERVLDADPAVVMYEWLTHDPDLIEAEARLAEASTA
                     CSLLDAITLSAGFEWDQLSDGPRWQVGAAVAIGAGVVGESRRPATHEHGAGLQRRIDA
                     LRARSTARFVGALGHLQSVWAEFLRLSEPESGNPGEAQIDARRIAEVAGIAFLAGLGV
                     FIWKSQ"
     gene            complement(10465..11925)
                     /locus_tag="PLM74_00765"
     CDS             complement(10465..11925)
                     /locus_tag="PLM74_00765"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HON41649.1"
                     /translation="MVTHVVVPGDTLYAIARRYGVSIESIVAANAISDPNVLVVGQAL
                     AIPVTRVPVEPLVYTVRPGDTLYVIASLFSTTVQAIAAANAIVNVNLIHVGQRLVMPG
                     WTHQLYTVAAGDTLSRLASRFATTVALLATVNRLADPNLISVGQSLVIPVKSAPVAPK
                     TPIETNGYLFPLSEGSTRRILSGISHDLTYASVFSFPVDGRGGVVYTAGASGPAVRVA
                     RSLGVAPLAVISNFDGSNFTPELAHNAMSGPTGDATIESIVEFALAEGFQGINIDFEN
                     MHPEDRQLYTSFMAALSRAAHARGLSLSNAMAPKWADWPSLPWVGTFDYAALAPHLDF
                     TMLMTYEWGWSGGPPMAIAPVNLVRRVLEYAVTQIPPGKILMGIPLYGYDWTVPAPPG
                     TFARTVTPSQAVDLASRYGVNISFDQTAQAPWFRYVDEAGAQHEVWFEDVRSSVAAHA
                     LIGAFRLRGASYWNVMYDYPQNWLALEDRYTVRKQS"
     gene            complement(11997..12884)
                     /locus_tag="PLM74_00770"
     CDS             complement(11997..12884)
                     /locus_tag="PLM74_00770"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HON41650.1"
                     /translation="MVSPGVDAARALDVVSLIVREARVEQDAVSIWRAVARQTTECMG
                     YRAAALGIIDSDGVLQFTFYGDVPDRHDEGACRQTPACAPDGSWISAPLTGIGRSVGI
                     LIGYGHGTGAFAADDLMIHAKLCDCLAVVIEAQMAVANLVQALDRDPVTGLYNRKHLV
                     RRIREETSRAERYGGQFALVFLDIVGMREINAAFGHGMGDAAIEAVAGAVKGSFRGSD
                     VAARFGDQTFAVLLPGADKPRGQIVANRIKRIAAGIPVTSDGARMQGPDLEWCVVSYP
                     ADGSTCDQLLAAYEHGAMM"
     gene            complement(12956..13237)
                     /locus_tag="PLM74_00775"
     CDS             complement(12956..13237)
                     /locus_tag="PLM74_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41651.1"
                     /translation="MLYPQAMMDDPGYAAGAVTAPDAQSGLDAAQFFPWVRPFWGFPG
                     FGFPGFGFPGWGFGPWGFGRPWGFGFGPWGFGRPWGFGFGPWGFGRPWF"
     gene            complement(13352..13870)
                     /locus_tag="PLM74_00780"
     CDS             complement(13352..13870)
                     /locus_tag="PLM74_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41652.1"
                     /translation="MRQKASLMPLVFSLATLLAAALIVSVVVPGFGGGAGSAQAASLP
                     YLSEALNQEAKMSVAELAAIRVTAYYNCPGALTTRLVRQSARCFLGPTSIDLFVDTRT
                     QPEWDTHLGAARFSVSDAEVAAAYGEAGAVATEWLTRFFPGVAPGSMRVIFTVKGYQI
                     GIYSTGRFTIVR"
     gene            complement(13867..15165)
                     /locus_tag="PLM74_00785"
     CDS             complement(13867..15165)
                     /locus_tag="PLM74_00785"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HON41653.1"
                     /translation="MSLFVARGRACEHEKSSSVRHNIRVNAEHGAWQSLAANLYSPFL
                     GVLAIKLGASNLQVALISALPAAVSCIASVLGAQVLRPFERKKGAACAFAFANRLFLL
                     GIAALPLFSEDSRAGALVLLIALMNVPGAIGNIAWQALMADAIPSERRGEAFAMRSRI
                     VTAVGFLPTLVGGYLLDALSFPIGYQVVFVVAFAASVMEVRALSKTYEPRCDVECVPI
                     ASNSGGSRTFDRRALLGQIARAGFTPRAMLFYFGWMMAQPLFTIYYVRVLDCGNLWVA
                     VFSIVSSMAQYLSFPVWSRYAARKGNTAALAAATAGMALTPVMVAISPRPWMVAAFNV
                     SMGFFTSGTTLLMLNTLLEVSPAENRTDFIAYHNAAVNLTSFIGPLIGRALVDSLNIR
                     WALVMAGAFRAAGSLAFAALSRRSEHEALHVTGPRGGEAC"
     gene            complement(15162..16187)
                     /gene="add"
                     /locus_tag="PLM74_00790"
     CDS             complement(15162..16187)
                     /gene="add"
                     /locus_tag="PLM74_00790"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HON41654.1"
                     /translation="MGECTDAGMRSLPKVELHRHLEGAIRPATILDLFQKNRREYLDT
                     PLCEILPRVQITGSDRSLADFLTKFEFFMPCIQTASDISRITSEAIEDCAADGIVYVE
                     LRFSPAFIRRLTGLSYSEAVEAVLQGARVAAAHDIAVNFILIAPQPEGERVAWDTVRL
                     AADYLSNGVTGVDIADDTRALGLEEYARPMAWARANGLGVTIHAGEAEGPSSVKVAVE
                     QLGATRIGHGVRAIEDDRVVKMLVDRGTLLEVCPTSNVHTQAAPSVREHPLPRLIERG
                     VSVCLNTDDPGISNITLTGEYVLVEEAFELSRADLALMNVRAARHAFASDEQRAALME
                     RLGESAS"
     gene            complement(16256..>16396)
                     /locus_tag="PLM74_00795"
     CDS             complement(16256..>16396)
                     /locus_tag="PLM74_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012002323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4438 domain-containing protein"
                     /protein_id="HON41655.1"
                     /translation="VVHSDCLVAGHGPGVATIMTCASPVIEPVLDDTANIGKYLGIGR
                     YR"
     assembly_gap    16397..16406
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16407..17146)
                     /locus_tag="PLM74_00800"
     CDS             complement(<16407..17146)
                     /locus_tag="PLM74_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012002323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4438 domain-containing protein"
                     /protein_id="HON41656.1"
                     /translation="MRTNKDRLVMISVQGAVAPHVRKQAFRIDADGVPFALPGVGGIT
                     YNVRVGDAAFGWAGDHIEPGVSTAAKFEKRADELNRAYNILSCIGNEARVVSGDAKGA
                     RGVVTGHHGGIEHVLVDFDDDTLEKLCIEDKILIRSYGQGLRLLDYPDVKLFNLDPAL
                     LELMNPEEAGGRLRVRVTAVVPAVLMGSGIGSADVASGDYDITTTDRAEIARLGLDKL
                     RFGDIVALADCDNLYGRSYRRGAVSIGVV"
     gene            17313..17504
                     /locus_tag="PLM74_00805"
     CDS             17313..17504
                     /locus_tag="PLM74_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003396016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HON41657.1"
                     /translation="MDYHHPTGRGCMELARAYVCPQPAYSQRYNPIEGLIHGTIFPEL
                     VRAYVPERERPVCPREVGK"
     gene            17513..17764
                     /locus_tag="PLM74_00810"
     CDS             17513..17764
                     /locus_tag="PLM74_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HON41658.1"
                     /translation="MNKDELMLRIQALEFTAVDLNLYLDTHPCDARALADYNQVAAAL
                     AQTIAAYEQMYGPLTNFGRSQSCGRWAWVEQPWPWETQG"
     gene            17795..>18057
                     /locus_tag="PLM74_00815"
     CDS             17795..>18057
                     /locus_tag="PLM74_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HON41659.1"
                     /translation="MWVYEKKLEYPVKVSGPNPKLAKAVITQYGGPDGELGASLRYLT
                     QRYSMPTSQAKGVLTDIGTEELAHMEIVAAIVYNLLKNAPLEQ"
BASE COUNT         3885 a         5762 c         4960 g         3440 t
ORIGIN      
        1 cgatgaccac cagaagcggc acgaacggga aaagcatcac gatgtcggct aatcgcatga
       61 agagcgtgtc cgtgaatccg ccgtagtacg ccgccatcgc cccaatgaac gtgcccacca
      121 ccacgctgat caccgccgat agcacaccca gcaggaactc acgtccggta ctgaacatga
      181 gctgcgccaa gatgtcgcgc ccgatgggat cagtgcccag caaatggcct gccatgggcg
      241 gtgacgggtg catcagcatt gggtcgaaac ccacaaccgg gtggtagatg agcggatccc
      301 acacgaagct gatcatgatg ggccacagga gcccgaagat tccgaacgca atgatgatga
      361 tcagcccgga catgcccact cggctctcct tgaatatctc ccagttctga gcgagcgagc
      421 gcatgttcat cttccatcgt atcttccaga taggctccgt tatccccggg tcaaaccggg
      481 gcagatgctc ctcgggccct ctcccaacgt tggggctggt agctaggtct gtcatgcgca
      541 ttcacctacc cttccgccgc cggttgcccg gtgtaggata ttctgggatc gaggaaggcg
      601 tagagcacgt cggcaacgaa gtgtgctacc agcaaaaaca cgccggtaaa gacgagcgcc
      661 ccggtagcca gcggatagtc attgcccagc gccgcacgga aaagcacctg cccgatcccg
      721 ggccaggaga acatcgtctc tgtcaccagg ccgccgctca tcgacgatgc caagctcagc
      781 actaagcttg tcactgtggg aagaagagcg ttgcgcgcgg cgtagcgatc gcgcaccaca
      841 ttgtcgggca ggcccttggc tctggccgcc atcacgtagt cttcctgaac cgtctcgagc
      901 atagaatcgc gcatcaggag catcgttccc gcgaagttga tcagcgtcag cgtacataca
      961 ggcagtatca tgtgtcggat gatgtcccat atgtagatta gcgtcccggt agcgtgcgca
     1021 atccagatcg ccaggccgat caccccggct gtcgacgccc atgccagcgc agcgctcttg
     1081 cgcgttctgc gcagcccgcg cctgaaaacc cagaatatga ggaacacagc gagagcgatt
     1141 accgaggcgc ctagtaccat gtagccgaag accgtattgg agctaaacgg cgccttgacc
     1201 cacaaggcag gctcaatgaa ctggttcaag ggtagccagt gcagagtgaa ggcgaacaac
     1261 cagatcaaga tcagcgacag cagcgggtag aaggctgtcc agaatgttat gccgactacc
     1321 gtggcgatgt ggtccacagt cgctccacgc tgccaggcta tgattcggcc gagaacaaac
     1381 ccaacgtaaa acgacaggca gttcgcggtg acgaagagca cgatagtgcg cggaagtcgt
     1441 tcaatgagga tgtcccatac aggcctgggg tagtggctga aggacacgcc caggttcccc
     1501 gtgaagaagt tcttgatgta gctgaggtat tggaggactg gcggtcgatc cagacccagt
     1561 tgcttcttga gctcctgccg cgcggcctgc ggaatccttg ggtttgccag gtacacctga
     1621 gatatgtcgc ccggcatggc ctgcatgagg aagtacacca tggtcatgta tatgagcaac
     1681 gtgagaacca actggcctac acggcccagc atgtatcttc gcattgttat cacccgagct
     1741 ttcagctgtt ctcttccaaa tcggagcgcg ggccgacagg ccccgatggg ccgccggccc
     1801 gcgcgtggcc aggaatcacc cagcgaacgc tcttagttct tcagcttgac tgtgctggtc
     1861 atgccgccaa agtactggat gccgctcagg acctttgtga atgggtactc cagacgatca
     1921 cttctatagg cttcgatcag aggagtatcg aacagcacta tgtacggcag atcctccgcc
     1981 agcatctgct gcatccggaa ggccttatcc cgagccttga ccaagtccgg ctcggcaagg
     2041 aacgactccg acagcttgtc gaactccgga ttgacgtact gggtggcatt gaatccaccg
     2101 gcaggcgcct ggctgctgtg gaagaaccag tgcatatggt cgggatacag gctcaagctc
     2161 caccccagca ggaaggcatc gtagtcgcgg tcgtcagtcc tggtgaggac cacgttgaac
     2221 tcagtcggaa ccacctcgca gggtatcccg atctcctgca tccaggtacc gatgttcagg
     2281 ccgaatgtgt agcgaagcgg gtcatagccg gccgtcatga ccatgaactc gaacgacggc
     2341 atcttcttgc cgtccggcat gatcaggccc ttgccgcgct tcagaacttt gtctttctcc
     2401 acgtctacct gaggctcaac ctcccatgag aacccagcgc tcttgagcaa cttgactgct
     2461 tctacaatcc gctggctgcg ggtcagcccc ttgccccaca tcttgacatc ggtgttgaac
     2521 cagtaggcgt tgcccggagg aactaccgag tactgggcaa acgccacacc ctgtaacacc
     2581 ctctgagtga tgtactcgcg gtcaatcagc gtagctactg cctgacggaa ctccttgatc
     2641 ccgaatgggt aacgtgcgag gttgaacccc atgtatctga aaccgtttac actgttctcg
     2701 atggtggtga tgttaggcgt cttcttgagc tgttcctgga atccgcgctg cagacccagg
     2761 gagttcagca cgaagtcgac ctcgcccttc atgagtgaaa gcactgccgc gttctggttc
     2821 tgcagaatgc ggaacctcag ctggtcgacg tatgggcctt ccacgacctg aagcttaatg
     2881 tcgcccttgg cgtctcctgt agtccacttc acgccggtct ttttgttctc gatcatgaaa
     2941 ccgccgctcg tgtagaaggt gttcgtgtcg cccttgaagg tgtagtactt gttaggcgta
     3001 tgcgtgacga acgcgccctt ctcccagcgc tcgaccaccc acggacccgc aaccggctcg
     3061 cccaccggat catgggcaag cagttccttc acagggtcag ctgccttaag ggctttgtca
     3121 actatcgggc cccagtatgc cttgggcaag atgtacgcct gcagcatgcc atactgccac
     3181 tgcgcgagcc caggcagttc ggtgaggaag aacttcacgg aatagtcgtc gaccttctca
     3241 acgcgggcaa ggatgtccgg gtcaatgttg ccgggccagt tgccgccgag cttgttaggg
     3301 tcgagcgcca gcggcgccat gtagctgaac gcaacatcgt cagcggtaat gggcttgccg
     3361 tcgctccact tgaggtccct gcggatgcgc actgtggagg tgtagaacgt cttgccgtct
     3421 atcttctctt cctagaaggg cgtcggcatg cccagcgctg cggacggact gaacacaaag
     3481 tccggtgcag cattgccgta aaggccgatg tacgattgct ggtaaccaac gaatgcattc
     3541 cagaccgagg catcgggccc gagctgcgcc cagatgttca gcgtggtggg gtcctcgaaa
     3601 atcgccatct catagacctt cgccgccgcc tggcccatca cgctcatgcc cgctatcacg
     3661 gccacgaaca gaagaacagc cagaccaaac cttaggtttt tcatagcccc catgtacctc
     3721 ctttgtactt aagtggagtc acagggttat gattctgcct aaagaatcaa agtcctgcct
     3781 ttaagcatgg ccgaatatac ttgcgtaccc atatacgtca tagcgcttcc accaatttct
     3841 tcgtcacctc atcctccgcg cgcttgtccc agccctcttt cacgtacgaa accgctcctg
     3901 acctgtcgat tacatacacc acaggcacgc cgcgcacacg gtatgacgcg gcagccgacg
     3961 ggcccgcagg cccacctacc accgggaatg ttgctccaag cccggacacg aactggctga
     4021 tgactcgcgc atcctcgccg agatcgttta tgcccacaca gagcagcccg cgagcgcggt
     4081 gccgatcatg cagacgctga atggcgccca gagcgtcggc cgacagccga tacttcgtgt
     4141 tccagaacac aaccaacatc accttgcctc gcaccggtat gacgacttca ccgccatcga
     4201 gcgaggtcgc tcttacctga ggcgcagatc ctccaagcag actcgccggc tgcgccagaa
     4261 cttcgcctcc accaaacgcc agccctaccg caatggcggc cagcgtaagc agggtagtgg
     4321 ccagaactcg cctgtgtctg atcattcgca ctcaccttcc cgagatcgcg gagcgatttc
     4381 gcacatctag cgcgctcctc gtacgtacat ttgacgcctg cccaccgctg aggtttcatg
     4441 ctcgactcat tcgccaggcg cctgatcgaa aaagctcccc cttccggggg agcgcttcat
     4501 catcgctctc tacttttggg actcaggcct caatggcatc cacaccgtcc tgcccacgag
     4561 ttcggagtat ctatactccc cttcacccga cgcaaacgac tcccgaatcg tcctctcaaa
     4621 cttgttgacc tgcgcatcca tgttgtcggc atggtgaaga atgcacgcct caattgtcgc
     4681 aggctgaaca ggagatcccc attcgagagt gccgtggtga ctcgccacaa ggtgggcgat
     4741 ttcgagcgtg agcccaggtg tccagcatgg cgaagcgcac gagcccttgt tggcacagtc
     4801 agacaccagc tcagcgatgc gcctcgagac caactccatc ccgagcacga tgtggtcgac
     4861 cgccacgccg ttcggagaca tcgccgcttg agcccgcgcc cgatcgtaat catagctttg
     4921 gaccttgccg atgtcgtgga ggagcgcgcc ggcgcagagg acatcccagt tcaccgaagc
     4981 gtactgctcg gccgcctggc gacaaatccg caagacgttg acggtgtgct ccagaaggcc
     5041 gccacagtat gcatgatgat gcctgatcgc ggcgggccac tcccggaacc tggccatgaa
     5101 ttcggaatcg ccaaagaacg attgaagaag acaacggatc ggcctattcg acacgccgga
     5161 cacggcggcc gtaacctccc ggatcatctc gtcaacatct cgtggagtcg cagggacata
     5221 gtcagccagg ttgcactgtg acacatcaca cagtgtgatg gaacgaccga aactggatgt
     5281 gtcttgcgac agctgcaggc tcccgccgta ctcctccacc tgaacgttgc taacgcgcac
     5341 acaggcgcct ggctcaagcc ccagagtggc gtcaggcgaa taccgccaaa gttttaggct
     5401 aatctcgccg gtcctgtcaa ccagcctcac atcggcgaaa tacgaaccgt tcctggtcat
     5461 cctggatttg aggtccgcac aaagaaagac tgtgtccgcc atcatcccag gtctcagatc
     5521 agctacgaac tgcgttttga ctatccggtg ctccattaga taacctcatt tcgctgtcac
     5581 tccactctcg ggccgaagaa ccgctctatc tcccgaaaag ctgcctcccg gctgtcggcc
     5641 gcgtgtgcca gattgtacgt cacagaagta gcaaggctgc cgcgcaccgt tcccggcgcc
     5701 gctttcatcg ggtcggtagc gcccagtatc tgccgagcgg cctccaccgc attcggagct
     5761 tccatgacca tggcaagcac gggtccggac atcatgtacg ctttgatctc gtcgaagaac
     5821 ggttcgtcgg ctaggtggag gtagtgctcg cgcactagcg cttccgacat caccagagta
     5881 cgcgccctca ccacactaaa cccgcgggtt tcgaacatcg atatgatctt ccccgcaagt
     5941 ccacgcatca gcccgtcggg cttaatcatg acaaacgtgc gctccatggc gcgcctccca
     6001 cctctatgga atcagaccct ggtcaccgtc aatggccctt gccagattga ccaggtcctt
     6061 cagagccgaa ctagctgctg ccgacggccc tccggcgccg cccacaattg tgagttcgcc
     6121 gaacatgtct gtcgtgaatc tcacgccctt gctatcgcct gtgacctggg ccagaaaatc
     6181 cttgtatgga acgggcgtgg gcttcacctc gatgctgaca tcatcgccgt tacggactgc
     6241 ccttcctatc agccgcaacg tacgaccctg caccgcccag tacgctatct gcctgggggt
     6301 aacttcggtt atccccgtaa tcttcacgtc gctgagtcgc ttacgggctc ccattgccgc
     6361 gttggccaaa actagtattt tacaggccgt gtcgatgccg ctgacatcgc gctcgggatc
     6421 ggtttccgca aggcccaaag cctggcttct acgtaaggcc tcctcataag agagtgcatt
     6481 gtcggacatc gctgtgagaa tgtagttcgt agtcgagttg agtacaccct ctatcgaatg
     6541 gacttcagcc cctgcaaggc tcaccctcac agtatcaata gtaggaaggc cggctgcggt
     6601 ggcgccgctg aacttgagtc gcacacgcgc gtcccgggca gcctgcacca tgccgtcgaa
     6661 gtccgctaca agaggggcct tgttgagggt caccagatgg caaccacgcc gcgcagctcc
     6721 cttcagcctg gaaagcgcgg gttcgccggt cgccagatcg gaccaggttg cgtcgaccag
     6781 cacccgtata tgccccgatt cctccgacgg aagccacagg tcgagatcat cggggtttcc
     6841 gcctacaaaa ccctcctgct cagatagtgt gcccgcatta gccagattca ccagaaccgg
     6901 ccattcgatg ccggattccg cccggattcc gccttttcgg gtgactatcg acaccactcg
     6961 cggctcaatg ccgtggcgtg atttcaggta cgacctcttc ctgttgacca gcctcacaaa
     7021 ctctcgcccg acattgccga aaccatagag aattatcgcg atctgcaaga tgataccccc
     7081 tagacctgcg gttctccctt ttttactccg gccatgaaca ttagagcgat gagcgcacac
     7141 agtaccgcga acggaaaaag cgccctcatg ccgaaaagat cgcgtagaaa gccaaagacg
     7201 ggcggcccgg tgatagccgc gagagacgag aacaggtaat ataggcctgt ataggcgccg
     7261 gtcttggact tagcggccat atctacaacc atcggatatg agttgatgtt tatcgctgcc
     7321 cagaacatac ctgccagcgc cagaacggcc gggactatca cagatgctcg tgcaagtatc
     7381 gcgcctacaa tagcgagctg accatcgccc tcaaccgagc ccgcgtagaa tagcttgttg
     7441 agcagaacca acacaggccg gccaagcgcg tcgctgccca ccaggcctat gatagacagg
     7501 cagactgcca accccacgat gccgatcttg atggtacgcg cccgaccgat ggatgtagcc
     7561 aagaaccctg cgggtatggc gaacgccacc agagcaagcg agaagaatcc aaggtacaac
     7621 gccccgctgg cctcggatat gccccatacg tccttgccgt acagcgtgaa gaaagcctcg
     7681 attccgttcc atgcaatgaa ccagaagaag atggcgagaa gcatgcacct ggcactgcgg
     7741 tcgggatcac gcctcacttc gccgagggct tccaggatcc ctactgtctc actgccctca
     7801 ccggcgcctt cgacgacctc cggttcgcga attcgccact tgagcaccag agtaatgatg
     7861 atcatcaaga ggccggcgaa tacgaaggga agtgccggat tcacattgta cagccgcgaa
     7921 cccacgaaaa aggcgaggag tgccccgagc cctcccatca ggttgatcac gccatttgcc
     7981 ttgcttctga gcgccggcgg cgtgatatca ggcatcaaag ccactgtggg agctcggaaa
     8041 atggccatgg ccaggttcat tgctatcaaa gccgtcatca ttgaagccag agaagatcga
     8101 aacagaggaa tgacagcgaa gaagagcgct cctaggggca tgcccgcgag tatgtacggc
     8161 atacgccgtc catacctgtt ccacgttcta tcgctggcgg cgccgaagta gggctgcagc
     8221 gtgaccgcgg cgatgttatc gaacgtcatg atgaaaccga tgagcgtgcc tttaagcacc
     8281 gtatctgcca agacattgtc gaggaagacc ggcacaaacg cattgtacaa cgcccagctc
     8341 aggctgattg cgaagaaccc gagccccaga gtgaacgtgc gggggtagtc gagcttccga
     8401 gtcatggctg ctccgattcc tcctctcgca tcttcacggc caacggtaca tcgttagtaa
     8461 tgatcgcatc gacgccggat gccagcatgc ggcttatatc ggcggggcta tcgcaggtcc
     8521 acgcgttcac ctgtactccc gctgcatggg cgcctgccac gaactccggg aacacagtga
     8581 aatggatggg atgcaaagcc tcggcgccta atcgcgccgc ataatgccat ggttccacaa
     8641 gtgcgcccat gtacagaagc cctatggcca tctcgggtgc caaccgcttc agctcaacca
     8701 ggccataatg gttgaaagac gaaaagatca ctcgcttggc cgcgccgcgc ctgcgcacaa
     8761 gatccaccaa cttctcctcg atcccggggt acagtacaac gccgctcttg atctcgatgt
     8821 tgatcaggcg acatgcatct cccacggcgt caagcacctg atctagagtg gggatcctct
     8881 gaacctcggc tccatgtacg aacttggccg ccgcattcaa ctcacatagt tcggcgaagc
     8941 tcttctcccc aacaaacccg cttccatcgg tcgtgcgttc caggcgctca tcgtggatga
     9001 ccacaagctg gccgtcgcgc gatagatgca cgtccagctc gatgccatcc gatcccatct
     9061 caatcgcctt gcggaatccg gctaatgtgt tctctgggga aataacaggc acaccccggt
     9121 gccccagaat caggggcatc ctgttcaata tggatcacac cttcccacat gattcaggcg
     9181 aatgccgcac tactgacgcg gtatttgaca tcggcctcgg aaatcctctg tgagaaggtg
     9241 agcttctatt gggacttcca gatgaacact cccagcccgg cgaggaaagc gatgccggct
     9301 acctcagcta tcctacgcgc atcgatctgc gcttctcccg ggttgcccga ctccggctcc
     9361 gacagcctga gaaactcagc ccaaactgac tgcagatgac ccagggcgcc gacgaaacgg
     9421 gcggtgctgc gggccctcaa ggcatcgatg cgtcgctgaa gcccagcccc gtgctcatga
     9481 gtagcgggcc gcctcgactc acccaccacg cctgctccga tcgcaacagc cgcccctacc
     9541 tgccagcgag gaccatccga taactggtcc cattcgaacc cagcggatag ggtgattgca
     9601 tcaagcaggc tacaggcggt gctagcctcg gccaggcggg cctcggcttc gatcaagtca
     9661 ggatcatggg tcagccactc atacatcacg actgccggat cagcatcaag cactcgctca
     9721 atcagctggg cgagatgcgt cggaccatcg tcgctcatca ccacgggcct gatctcatcg
     9781 gaaggcagtc caatcagcag agagagttca ccgtacgcct gcatggcgcc gaccgcatcg
     9841 caccgcgccg ccagcacttc agccttccgc agcatgtcga ctgcccgagc cgcggccccg
     9901 gcctcgacca ttgcgactct gagctcggcc gccctcagca gggcttcctc cagactcacg
     9961 gcttcagccc ggataatccg cgggatgcac gagagcggcg cctcaaccac ccctctccac
    10021 gtcgccatcc cgcggttctc actcgagccc ggtctgcccg attcgtacgt gaggcggcag
    10081 gataccccgg gcggcccctc tgccgttgcc catgcttcaa gtgctgatga tgatgcacga
    10141 aaacccaaca cgggacgcca cgacggagct ccgctcgcgg ttccgaattc cgcagcaaag
    10201 cgatcactcg cctctgcaca gtacggatcg aggcgaatcg cagcatcgag gaactcacac
    10261 atgctcatat gaattggttc tgcctgtgaa aacatctgag atgtcgccgc caggagaagg
    10321 gtaaaaaggc agacgagcgt ggcgcgccgg cgaacgatgc gccctatgaa cacagatatc
    10381 acaagatcac ttcctccaag ccactgtcct gataacatac tagaacggct agagcggctt
    10441 gcccatcgcc cgaccgggtg attttcagct ctgcttgcgc acagtatagc ggtcttcgag
    10501 agccaaccag ttctgcggat agtcgtacat gacattccag taactggcgc cacgtaatct
    10561 gaacgcccca atgagcgcat gggctgccac tgatgaacgt acgtcctcga accacacctc
    10621 gtgctgcgcg cctgcctcgt ctacataccg aaaccaaggc gcctgggcag tctggtcgaa
    10681 gctgatgttc acgccgtatc gcgaagccag gtcgacggct tgcgagggag tcacggttct
    10741 ggcgaacgtg cccggcggcg ccggcacggt ccaatcgtag ccgtaaaggg gtatgcccat
    10801 gaggatcttg cccggaggta tctgagtaac ggcgtactcc agcactcgcc tgaccaagtt
    10861 cacgggggct atggccatcg gcgggcctcc cgaccatccc cattcatacg tcatcagcat
    10921 agtgaaatcg aggtgtgggg ccagcgctgc gtagtcaaac gtgccgaccc agggtagact
    10981 gggccagtca gcccatttcg gagccatggc atttgagagg ctgagccccc gcgcgtgggc
    11041 agctcgcgac agcgccgcca taaacgatgt gtacagctgc ctgtcttctg gatgcatgtt
    11101 ctcaaagtcg atgtttatgc cctggaagcc ttcggccaga gcaaactcga cgatgctctc
    11161 aatggtcgcg tcgcccgtcg gccccgacat agcattgtgc gccagttcag gggtgaaatt
    11221 cgacccgtcg aagttggata tcacggcaag cggagcgact cctagcgagc gcgccaccct
    11281 caccgccggt ccggacgcac ccgccgtata cactactccg ccccgtccgt ccacgggaaa
    11341 gctgaaaacg ctcgcgtatg tcagatcgtg tgagattcct gagagtatgc gcctcgtgga
    11401 gccctcggag agggggaaca gatagccgtt cgtctctatc ggcgttttcg gcgcgaccgg
    11461 agctgatttc acgggaatga ccagtgattg tcctacgcta atcagattcg ggtcggccaa
    11521 gcggttgacg gtcgctaaga gcgcgaccgt cgtggcaaat cttgaggcca gccgcgacag
    11581 ggtgtcaccg gcagccactg tgtacagctg atgagtccat ccgggcataa ccagtcgctg
    11641 ccccacatgg atcagattga cgttgacgat ggcgttcgcc gcggcgatgg cctgcactgt
    11701 agtagagaat aggctggcta tgacatatag cgtatcccca ggcctcacag tatagacaag
    11761 aggttccaca ggaacgcgcg tgacggggat ggccagggcc tgccccacaa caagcacgtt
    11821 aggatcggaa atggcattgg ccgcgacgat cgactcaata gacactccgt acctgcgggc
    11881 gattgcatac agagtatcgc cgggtacaac aacatgggta accacgctca tcactcctgc
    11941 aggaaactgc ctatgtcatc atattggccc tcccgcagaa ggtgtccctc cggcttctac
    12001 atcatggctc catgttcgta cgccgccagt aactgatcac aggtgctccc atcggccggg
    12061 tacgatacca cgcaccattc caggtccggc ccctgcattc tggcgccgtc gctcgtgacc
    12121 ggaatacctg ccgcgatcct cttgatcctg ttggccacga tttggcccct cggcttgtct
    12181 gcgccgggga gcagcacggc gaatgtttgg tcgccaaacc ttgccgctac gtcgctgccg
    12241 cggaagctgc ctttaacggc gccggccacg gcctcgatgg ctgcatcgcc catgccgtga
    12301 ccgaacgcgg cgttgatctc gcgcatgccc acgatgtcca agaacactag cgcgaattgt
    12361 cctccatagc gctcagcccg cgaagtctcc tcgcgaatcc gtcttaccaa atgtttccgg
    12421 ttgtagaggc cggtaaccgg gtcccgatcc agcgcctgca ccaggttggc cacagccatc
    12481 tgggcttcga tgaccacagc caggcagtca cacagcttgg cgtgtatcat gagatcatcg
    12541 gcggcaaacg cgcccgtgcc atgcccatag ccgatcagga tgccgacgga acgacctata
    12601 cccgtaagcg gagccgatat ccaggacccg tctggcgcgc aggcaggggt ctggcggcac
    12661 gccccttcgt catggcgatc cggcacgtcg ccgtagaatg tgaactgaag cacgccatca
    12721 ctatcaatta tgccgagagc agcggcacgg tagcccatgc actccgtcgt ctggcgcgca
    12781 actgcccgcc atatgctgac agcatcttgc tctactcgcg cttctcgaac tatcagtgaa
    12841 accacatcta gagcacgagc agcgtcaacc cccgggctga ccatgcccat ccccccaacc
    12901 acatcgagca ggggggcgga tccccgccct ccctctctcg caatcagcgc ttacatcaga
    12961 accagggtcg gccaaatccc cacggcccga atccgaatcc ccacggccgt ccaaaccccc
    13021 acggcccgaa tccgaatccc cacggccggc caaaccccca cgggccgaat ccccagcctg
    13081 gaaagccaaa gccgggaaaa ccaaagcccg gaaagcccca gaacggccta acccagggga
    13141 agaactgcgc cgcgtccagc cctgactggg cgtcaggagc agtcacagcg cccgccgcgt
    13201 acccggggtc gtccatcatt gcctgcggat aaagcacgtg cgccacctcc gtatgaagtc
    13261 ttggaccacg cacgacagca tattcgccgg cgtgcgtagg cggactgctc ggattggcac
    13321 atgcgaaccg gacctgccct acctatctcg gctatctgac gattgtgaac cggccggtgc
    13381 tgtagatgcc tatctggtat cccttgacag tgaatatcac ccgcatgctc ccaggagcca
    13441 ctccagggaa gaaccgtgta agccactctg tcgccacggc cccggcctca ccgtacgccg
    13501 ccgccacctc tgcgtcgctc acgctgaaac gggccgcccc caggtgcgtg tcccattcag
    13561 gctgagtgcg ggtatcgacg aacagatcga ttgacgtggg gccaaggaag catcgcgcac
    13621 tctgcctcac tagcctcgta gttagggcgc ccgggcagtt gtagtaggcc gtgacacgta
    13681 tggcggccag ctctgccacg gacatcttcg cctcctggtt gagagcctct gaaaggtacg
    13741 gtaacgacgc agcctgcgcc gagccggctc ctcccccgaa gccgggtacg actacgctaa
    13801 cgatgagcgc agctgcaagt agggttgcca gcgaaaagac caacggcatg agactagctt
    13861 tctgcctcaa catgcttcgc ctcctcgcgg gccggtcacg tgaagcgcct catgctccga
    13921 tcggcgcgag agagccgcaa acgcaaggct tcccgccgcg cggaacgcac cggccatgac
    13981 cagcgcccag cggatattga gtgaatccac caatgccctg cctatcaagg gccctatgaa
    14041 agacgtcagg tttacggcag cattgtggta cgcgatgaag tccgtcctgt tctcggcggg
    14101 cgacacttcc aacagagtgt tcagcatcag caaggtagtg cctgaggtaa agaaccccat
    14161 cgacacattg aaagcggcaa ccatccaagg ccggggcgat atagcaacca tgacaggcgt
    14221 aagggccatg cccgctgtgg cagcggccag agcggccgta ttcccttttc tggctgcata
    14281 ccgactccac acgggaaacg acaggtactg cgccatactg ctcacgattg aaaagacagc
    14341 cacccaaagg ttgccgcagt caaggactct cacgtaatag atagtgaata ggggttgtgc
    14401 catcatccag ccaaaataga acagcatggc cctgggtgtg aaacccgctc tcgcaatctg
    14461 tccgagcagc gcccggcgat cgaaggttct cgaaccgccc gaattcgagg caatcggcac
    14521 acactctacg tcgcaacgcg gctcgtatgt cttcgaaagt gcccgaactt ccatgaccga
    14581 ggcagcaaac gcgaccacga acaccacctg gtaccctatg ggaaacgaca aagcgtccag
    14641 cagatacccg ccgacaaggg taggaagaaa gcccaccgcc gttactattc tgctgcgcat
    14701 ggcaaacgct tctccgcggc gctcagacgg tatcgcgtct gccatcagag cctgccacgc
    14761 aatgttgccg atggcgccgg gcacgttcat cagggcgatt aggagcacaa gagcgcctgc
    14821 gcgcgagtcc tccgagaaca atggaagtgc tgcgatgccc aggagaaaga gcctgttggc
    14881 gaaggcgaac gcgcacgctg ctcccttctt ccgctcgaac gggcggagta cctgtgcccc
    14941 cagaacactg gcgatgcatg ataccgctgc cggcaatgct gatatgagcg ctacctgcag
    15001 attgcttgcg cccagtttga tcgccaggac gcccaagaac ggactgtaca agttagccgc
    15061 gaggctctgc cacgctccgt gttcagcatt gacccggata ttatgtcgga ccgatgaact
    15121 cttctcgtgc tcgcatgctc tgccccgtgc cacaaacaag ctcaactggc cgactccccc
    15181 agacgctcca tgagcgctgc ccgctgttcg tcactggcaa aggcatgcct ggcggcgcga
    15241 acgttcatga gggcaagatc tgcacgggat agctcgaacg cctcctccac gagtacatac
    15301 tcgcctgtga gcgttatgtt ggatatgcct ggatcgtcgg tgttgaggca gaccgacacg
    15361 ccgcgctcaa tcagcctggg cagaggatgc tcccgaacag acggcgccgc ctgggtatga
    15421 acgttgctgg tggggcagac ctcaagcaac gtaccccgat ccacaagcat cttaacaaca
    15481 cgatcatctt ctatggcccg cactccatgg cctatcctgg tggcacccag ttgttccaca
    15541 gcgaccttca cggaagacgg gccctcggcc tcgccggcat gaatcgtgac gccgagcccg
    15601 ttcgcgcgcg cccaggccat tgggcgcgca tactcctcaa ggcctagcgc tcgggtgtcg
    15661 tcggcgatgt ccactccggt tactccgttc gacaagtaat cggcggcgag cctaaccgtg
    15721 tcccaggcca cgcgctctcc ctcgggttgc ggcgcaatta ggatgaagtt cacggcgatg
    15781 tcatgcgcgg cagcgacccg cgccccctgg agtactgctt ccaccgcctc agaatacgac
    15841 aagccagtca ggcgcctaat gaaagccggc gagaacctga gctctacgta cacaatcccg
    15901 tcggccgcgc aatcctcgat ggcttcagag gtgatccgcg atatgtcgga tgccgtctgt
    15961 atgcatggca tgaagaactc gaacttagtc agaaagtccg cgagcgacct atcagaccct
    16021 gttatctgaa cacgaggcag gatctcacac agtggagtgt ccaggtattc ccgacggttc
    16081 ttctggaata gatccagaat ggtcgcgggc cttatggcgc cttccagatg cctgtgtagt
    16141 tcaactttag gaaggctccg catgcctgcg tctgtacact cgcccactgt catcccccca
    16201 aacgtgcacc gagcggcggt gcgacaggcc tgccgccgcc cgtgcagcag tcttgctagc
    16261 gatagcgccc tataccaagg tacttcccga tgttggcagt atcgtccaac acaggctcta
    16321 tgacgggcga cgcgcatgtc atgatagtgg ctacgccggg tccgtggcct gccacaaggc
    16381 agtcggaatg cacgacnnnn nnnnnnacga cgccgatact caccgccccg cgtctgtagc
    16441 tcctgccgta gaggttatcg caatcagcga gagccacgat gtcgccgaac cgcagcttat
    16501 ccaggcccaa gcgcgctatc tccgcccggt cggtagttgt gatatcgtag tcgcctgaag
    16561 ccacatccgc tgagcctatt cccgacccca tgagcaccgc cgggaccacg gcagtcaccc
    16621 taacgcgaag cctgccgccg gcctcttcag ggttcatcag ttcgaggaga gccgggtcta
    16681 ggttaaagag cttcacgtct gggtagtcca gcaaccgaag gccctgaccg tatgagcgga
    16741 tgaggatctt gtcttcgatg cagagcttct ccaaagtgtc atcgtcgaaa tcgacaagaa
    16801 cgtgttcgat cccaccatgg tgcccggtca cgacgcctcg agctcctttg gcgtctccgc
    16861 tcaccacgcg ggcctcattt ccgatgcacg acaggatgtt ataggcccga ttcagctcgt
    16921 cggcgcgctt ctcgaatttc gccgccgtgg acactcccgg ctcaatgtgg tcacctgccc
    16981 agccaaacgc tgcgtcgccc acgcgcacat tgtaggttat gccgcccaca cccggcagcg
    17041 cgaacggcac tccgtcggca tcgatgcgga acgcctgctt cctcacgtga ggcgccacgg
    17101 cgccctgaac agatatcatg acaagcctgt ccttgttcgt cctcaaaaca acggccacct
    17161 tccttctgct cactctgtac aaccttcgtt attcacctta tgggccgcga ttcctgcctc
    17221 gggcaggcgc aaatgtgctg accgcccgcc gccgactgga cgtgcctgaa tatgatggac
    17281 tggatcaaca cagcgtcaag ggaggaaagc atatggacta tcatcatcca accggacgtg
    17341 ggtgcatgga actggcccga gcatacgtat gcccgcagcc tgcatattca cagcggtaca
    17401 acccgattga ggggctgata cacggaacca tcttccccga gctggtgagg gcgtacgttc
    17461 ccgagcggga gcgcccggta tgcccccggg aggtgggtaa gtgatgcggg ctatgaacaa
    17521 agacgagctc atgctgcgaa tacaggccct cgagttcacg gccgtggacc tcaacttgta
    17581 tctcgacact catccgtgcg acgctcgtgc ccttgccgac tacaatcagg tggccgcggc
    17641 acttgcgcag acaatcgctg cgtacgagca gatgtacggc ccgctcacga atttcgggcg
    17701 gtcgcagtcc tgcggcaggt gggcatgggt tgagcagccc tggccgtggg aaacgcaagg
    17761 gtaaacatga ctagcggaga ggaggctacc agaagtgtgg gtgtatgaga aaaagcttga
    17821 gtacccggtc aaggtctccg ggcccaaccc gaagctcgcg aaggccgtga ttacccagta
    17881 cggaggtccc gatggggagt tgggcgcttc gttgcgctac cttacgcagc gatactccat
    17941 gcctacttcc caggccaagg gcgtcctaac cgacattggc actgaggaac tcgcacacat
    18001 ggagatagtg gcggcgatcg tctacaacct gctcaagaat gccccgcttg agcagat
//
LOCUS       DAOTAC010000009        17776 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_2627_length_17776_cov_4.383838, whole genome shotgun sequence.
ACCESSION   DAOTAC010000009 DAOTAC010000000
VERSION     DAOTAC010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 17776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17776
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2627_length_17776_cov_4.383838"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="PLM74_00820"
     CDS             <1..261
                     /locus_tag="PLM74_00820"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009628163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HON41660.1"
                     /translation="KALGDASGLWYTLDWLHACTFALVVACCGQLGDLAESAMKRDAG
                     IKDAGWILPGHGGILDRFDSFFFAAPVAYYYLILIVPGLGVM"
     gene            278..1321
                     /gene="ytvI"
                     /locus_tag="PLM74_00825"
     CDS             278..1321
                     /gene="ytvI"
                     /locus_tag="PLM74_00825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HON41661.1"
                     /translation="MSNHAKASIAVGALAIGMYLAIRFVLPLFTPFIVALFIAILIDP
                     VVSFLERRLRMPRAVAVIIVLAALGVVLGLLVAVGVSEISREIDQLLRNIGGLSKSLA
                     NVVDDLTARAARFFEGLPQPVTDILRANEGRIVALVETAASGMAGFLGRLPQFTLTLV
                     ISIVATFFVSRDFYSASTRAASALPRAWRKQFGKIKGEMLAAFVGFLRARFVLLSITA
                     VMTILGLSVMRVNYAWLLGLACGALDILPLVGPSALFLPWAFYHILVGNIGFAFGLVA
                     VLALSSAVRQIAEARVMGRNLDLHPLVALISVYVGVQLFGVKGFIAGPLAVIFFKAIL
                     HSIILPVFPLDKE"
     gene            1325..2488
                     /gene="dxr"
                     /locus_tag="PLM74_00830"
     CDS             1325..2488
                     /gene="dxr"
                     /locus_tag="PLM74_00830"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HON41662.1"
                     /translation="MLSVCILGSTGSVGSQAIEVARSLSLPVTGLSTWSNVRLLAEQA
                     REFRPRACAVGDPRMAGDARNLLRELPGAPQLFVGTEGLSELASMAEHEIVLNAVVGF
                     SGVYPTLAAIEAGHDVALANKETLVAAGSIVMERARRRGVSIRPVDSEHSAIWQCLAG
                     QDRQSLSRLILTASGGPFRDKVDLSDVTREQALAHPNWRMGPKISVDSATMINKALEV
                     VEAHWLFDASPDIIEIAVHRESIVHSMVEFVDGSVIAQMGAADMRTPIQYALTYPKRC
                     EGLAQKLDFAELGTLTFAAPDAARFPGVLLGHQALQLGGVAPAAMSAANEAAVDRFLR
                     GTLRFCDIAPVVAEAMHRSVDGGELSLRGIVEADGQARQFVEERCGGCTRSLS"
     gene            2467..3507
                     /gene="rseP"
                     /locus_tag="PLM74_00835"
     CDS             2467..3507
                     /gene="rseP"
                     /locus_tag="PLM74_00835"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HON41663.1"
                     /translation="MYALAFIVVVGLVVLVHELGHFAAARLAGVRVYEFAIGFGPRLF
                     AKQIGQTLYAVRALPLGGMCRLAGMDDSELESEQTDPDDPASFVNRPFWQRILVVAAG
                     PLMNFVLAIAIFTGLFSAYGVPMARVVDVMEASPAQVAGFRAGDTIISINGRPVASVA
                     GFVNTISTSWGRRLDVEIQRDGQRDTVHVVPEFDARAGVGRIGVSLTESPVKSEVRGT
                     VGGAIRYTWGVIDTTITSLWAALSGRAKPEVSGPLGIAGMSAQAAQQGLPSLLMFIAL
                     ISTSLGLLNLMPVPILDGGWIVLLAVEAVRGKKLSPQVEEALRFVGLALLILLMVYAT
                     VSDISRLIVREM"
     gene            3538..4575
                     /gene="ispG"
                     /locus_tag="PLM74_00840"
                     /gene_synonym="gcpE"
                     /pseudo
     CDS             3538..4575
                     /gene="ispG"
                     /locus_tag="PLM74_00840"
                     /gene_synonym="gcpE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075534.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
     gene            4646..5275
                     /locus_tag="PLM74_00845"
     CDS             4646..5275
                     /locus_tag="PLM74_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015908198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HON41664.1"
                     /translation="MRVSAIVPAYNEGPRIAAVLDVVRQSKMVDEILVVDDGSDDDTA
                     EVAALHGAKVVRMGHNFGKGGAVARGIQSTLGDVLLFLDADLVGLTVAHVEGLLVPVL
                     SDKADMTVGIFGDGRPATDLAQAITPWLSGQRAVRRSAFDGLDLCESGFGIEALLTRH
                     ARESGMRVVEVKLPRLTQVMKEEKLGPVKGAALRVKMYWDVLKTVVHRD"
     gene            5305..9033
                     /locus_tag="PLM74_00850"
     CDS             5305..9033
                     /locus_tag="PLM74_00850"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF001688.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HON41665.1"
                     /translation="MSTLLVMVPSDDRAELGHIFERAGMSAPEFLSPSCKIEKVDVYP
                     QSRQWVVHLVGDGDLSEECCEQVRLAFRKILGDRCDVSIRLSAENAGGIDSHTLEGCW
                     EQVARRVLDRAPSVGVWLGQARCRVVDDRVVVEVPGEVQRTKLAERGCADLISDALRD
                     IVGVRASVSIDVGEFDAPEVPDDDVCMQPDADARAALGSRPTPPVQPSPTGAERRGGR
                     RRRVVTDEGAVRGRRFTDAPQPLSSLVQGQKKAVASGEVFAVEERLTRTGLRIVSFCI
                     TDRQDSIACKCFCDPEEPVFELSEGQWARVRGDVQYDQYAREIVLAVSDIMPDSRPAR
                     SDTAPEKRVELHLHTKMSAMDSVCDVESVIRQAAAWGHPALAITDHGVVQSFPDAFAA
                     GKKHGIKIIYGMEGYLVDDAATDDSPTCHVTILAKNAAGLKDLYMLVSASHLHYFYRH
                     PRIPRALLAKARSNLLIGSACAAGELYRAVLGGASDDQLDHIAKFYDYLEIMPAGNDE
                     FLIRSGMLRGIDQVQAIAARIYGIGKRLGILVAATGDVHFLEPADEAYRRVLMAGQGY
                     EDADHQAPLYYRTTDEMLREFAFLGERASREVVIDNPGIIATQVECMRPVPGELATPQ
                     IEGADEEVRTRTYTKARELYGDPLPEAVRSRLERELTSIIGNGFAVNYDIAAKLVHKS
                     VEDGYPVGSRGSVGSSLVATMCGITEVNPLPPHYLCSNCRYSDFDHGVQVESGYDLPD
                     SQCPRCGSRLGKEGQSIPFETFLGFEGDKVPDIDLNFSGQYQSTIHKYAEELFGADHV
                     FRAGTIATVADKTAYGFVRAYAESHRLTLRNAEISRLARGCSGVKRTTGQHPGGVMIV
                     PKGRDVHEFTPVQHPANDRQSEVVTTHFEYKTIHDCLLKLDLLGHDDPTMIKMLEDLT
                     GISAGDIPMDDPATMSIFSGVEALGLDPEESGMTVGTVAIPEFGTKFVRGMLEATRPK
                     SFGELVRISGLSHGTDVWLNNAQTLIESGAARLTDVIACRDDIMNRLIFDGLAPKDAF
                     RITERVRKGKGLADGDDQLMRDHGVPEWYIDSCNKIKYMFPKAHAAAYVTMSFRIAYF
                     KVHHPIEFYACYFTIRASDFDSSIIAMSVQDIMEAMKATDGNQDATGREKSMAAIMEA
                     VAEARMRGVSFLPVDIAKSSATSFTIENGSIRLPLASVASLGDTAASSVVQAREERPF
                     SSIQDLRDRTRLTRSHVDTLRDIGALAGLPETDQLSLF"
     gene            9219..9704
                     /locus_tag="PLM74_00855"
     CDS             9219..9704
                     /locus_tag="PLM74_00855"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HON41666.1"
                     /translation="MDRRRIEIRARSIAESAAGPLGLNVVDAEFVRDSGYWYLRVYID
                     KPGGIAIDDCADLSTAIGAELDRQDFIPESYILEVSSSGEKPLRRDEEYDEFKGRWAL
                     INTYTEINGCKRFEGRLMGMVGDSVSMLVDGKAVEIPRRRISAARLAVPLREVCGDES
                     E"
     gene            9691..10728
                     /gene="nusA"
                     /locus_tag="PLM74_00860"
                     /pseudo
     CDS             9691..10728
                     /gene="nusA"
                     /locus_tag="PLM74_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014794860.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
     gene            10783..11061
                     /locus_tag="PLM74_00865"
     CDS             10783..11061
                     /locus_tag="PLM74_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HON41667.1"
                     /translation="MPKVRKTPQRTCVGCGKVTDKRSLVRIVRSPEGDVVVDPTGKRS
                     GRGAYVCAARECLQRALRTGKLSKALETAVPEDVVAALLGSLGGVDES"
     gene            11208..13937
                     /gene="infB"
                     /locus_tag="PLM74_00870"
     CDS             11208..13937
                     /gene="infB"
                     /locus_tag="PLM74_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HON41668.1"
                     /translation="MAKVRVFELAKELDMPSKDLVTLLNELGVKAKNHMSAIDENAAR
                     YVMRKYGPDAKKAESPGEQPTQDVSEAPTAPPADLPRAAVRAEPVRGPADRRVDAGPQ
                     DRPRPAPATRPQDAGRRQGRPQGVPGGQTRPADAGGRPRRPLDVGPQKPLDPSYPFIP
                     ARPRPQGAGAPRPRPFAPVTPITPVAPAPPQQALQQPRPERSDRFDRAERFPRPAGPG
                     PGFRRGPAVSVPPPPPSAVPAPGARGGGKGKQTWSGRGKRRGGLDDEMEAPRGDRRAD
                     RRGRKKSPTAAPLGDARLIERRVRAGDIDAADDVVVRRVGRRREKPTKVRRIEIGASI
                     GIRDLAARLEMSAPEVVSRLIKMGVMANVNQEIEGEIAQILATDLGFEAELTSEREER
                     EVAIAEIEDAPESLKSRPPVVTIMGHVDHGKTSLLDAIRETNVTATEAGGITQHIGAY
                     QVELGGRKITFLDTPGHEAFTAMRARGAQATDVAVLVVAADDGVMPQTVEAINHARDA
                     NVPIVVAVNKVDKPHAQPERVQRQLSEMGLVPEEWGGETVMVPVSALQKRGIQELLEM
                     ILLVADMRDLKANPDRPAMGTVIEAKVDRGRGPVATVLIQKGTLRVGDSFLVGEVYGR
                     VRAMANYRGEPLDEATPSMPVEVLGLSYVPEAGDVFQAVTDEREAKQVAEKRAERKRV
                     SEIGETSKKVSLEDLFEQIKEGQTKELRVIIKADVQGSAEALKQSIEQLSTDEVRVNV
                     IHSGVGAVTESDVMLASASNAVVIGFNVRPDAAGTASAEVEQVDVRLYRVIYEALDDV
                     KKAMEGLLEPEYREVVLGHAEVRATFKIPKAGTVAGCYVTDGKIARSARVRLLRDGTV
                     IHEGKISSLKRFKDDAREVAAGFECGIGIEGYSDVRTGDIIEGYSKEAVKREL"
     gene            13954..14235
                     /locus_tag="PLM74_00875"
     CDS             13954..14235
                     /locus_tag="PLM74_00875"
                     /inference="COORDINATES: protein motif:HMM:NF016351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HON41669.1"
                     /translation="MIIGRMHCRLAIDGSHSLKDKKRTLTSLLARARNTFNVAVAEVD
                     DLEMWNRAGIGVACVSNDSGHAHSILEKVARMFDSCPEVTLESYEIEIA"
     gene            14276..14656
                     /gene="rbfA"
                     /locus_tag="PLM74_00880"
     CDS             14276..14656
                     /gene="rbfA"
                     /locus_tag="PLM74_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HON41670.1"
                     /translation="MSSRRNDRVRAAMRQEISNMLQRDLKDPRLGFATVTDVRVSGDL
                     QHVQVYVSILGDDASRQQTMEALESARGYVRSEIGRRVQLRLTPEITFAYDDSIERGA
                     RLLRLISEVQSEETAARRDEENDN"
     gene            14659..15642
                     /locus_tag="PLM74_00885"
     CDS             14659..15642
                     /locus_tag="PLM74_00885"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HON41671.1"
                     /translation="MPGEIIEALGSARSVFITTHVVPDGDAIGSLLGMKLALERLGKQ
                     CEAGLSDPVPPSLRFLPGSAGVRSGGCEATGRYDLALVLDCGSLDRVGQCVETVEACA
                     VSVNIDHHATNTRFAAVNWVDTEAAATCQLVAELISAMGVAYDSDIATCLFAGLSTDT
                     GSFRYSNTRASTFRIAAELADAGARPWEIAQNVYDSRPYNSLAALAEAILGIEFSCDR
                     LIASMVITAADMARHGITEGDTEGFIGYARSIDGVEIAVALREVGPGTIRVGLRSKER
                     VDVAGVAGEFGGGGHVRASGCTIQGTMEEARRKLIPSLERALREAGYTWTA"
     gene            15630..16553
                     /gene="truB"
                     /locus_tag="PLM74_00890"
     CDS             15630..16553
                     /gene="truB"
                     /locus_tag="PLM74_00890"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HON41672.1"
                     /translation="MDGLIAIDKPPGMTSHDVVARVRRIAGQRSAGHTGTLDPDATGL
                     LVVCMGRATRLTRFMEGFPKEYEAEIVFGVSTDTGDDSGRITARSESFSIPLSEFLDT
                     LGRFLGDIRQTPPMISAVHHEGERLYELARRGITVERQPRTVTIFEIEPLSVVSWPSR
                     LEHGARGKLRIRCSRGTYIRTLCEDICRSLGIAGHMGALTRTATSGFTLDQAVTLEEL
                     EHLASLGSLAEVIKPPIEGVRHLPSLDLAEREADAVSHGMAFECDGNRIMNAPEDLVH
                     VALVRPDGNLAAVAAASRSELGLFIQPRVVL"
     gene            16584..17507
                     /gene="ribF"
                     /locus_tag="PLM74_00895"
     CDS             16584..17507
                     /gene="ribF"
                     /locus_tag="PLM74_00895"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HON41673.1"
                     /translation="MISRNQRPEHVAKVYALGMFDGVHIGHMALIQTAVRIAGERGTT
                     CGVYTFDVHPMRVVCPERAPDALMSLDARLDLMESAGVDTAVVHTFDTQLASMPPHAF
                     VSRVLHDALGAQCVVVGYDYSFGQGGAGTPATLELLAREAGICVQTLPPVRIDGAPVS
                     STGVRKALACGDVERAALLLGRRHFVRGTVARGRGVGRELGIPTANVSVPAGIALPMP
                     GVYACEARWGSSATVPAVCNIGVRPTFDDQGRGVTLEVHVMGVAPDLYGALLTVEFAA
                     RLRDELRFASPSELVRQIEADMIAARRLLEP"
     gene            17650..>17776
                     /locus_tag="PLM74_00900"
     CDS             17650..>17776
                     /locus_tag="PLM74_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013986613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HON41674.1"
                     /translation="MSLSIEEKRAIIEKYRVHDSDTGSPEVQVAILTERINYLTEH"
BASE COUNT         3566 a         4801 c         5869 g         3540 t
ORIGIN      
        1 aaggccttag gagatgccag cggcctgtgg tacactcttg actggctcca tgcctgcaca
       61 ttcgcgctgg tcgttgcatg ctgcggccag ctaggggatc tcgccgaatc tgctatgaaa
      121 cgcgatgccg ggatcaagga tgcgggctgg attctccccg ggcacggcgg tatacttgat
      181 aggtttgact cattcttctt tgcggctcct gtcgcatatt actatctcat cttgatcgta
      241 cccggtctgg gcgtgatgtg aacacggagg tgcccacgtg agcaaccatg ccaaggcaag
      301 tatagccgtg ggagcgctgg ctatcggcat gtatctggcg atcaggttcg tcctgccgct
      361 gttcacgccg ttcattgttg cgcttttcat cgccattctc atcgatcctg tcgtaagctt
      421 cctggagcgt aggctccgga tgccccgcgc agtcgcggtc attatcgttc tcgcggcttt
      481 gggagtggtc ctcggcctac tcgtggcggt gggcgtgagt gagatctcgc gcgagatcga
      541 tcagcttttg cggaacatag ggggtctatc caaatcattg gcgaatgtgg tggatgacct
      601 gactgcacgc gctgcccgtt tcttcgaggg gcttccccag ccggtaacgg acatcctgag
      661 ggcgaatgaa ggcaggatag tcgcgcttgt ggagaccgcc gcttccggca tggcaggctt
      721 ccttggccgc ctgccgcaat tcactttgac tctggtgatc agcatagtag ccacgttttt
      781 tgtgtcgcgc gacttctact ctgcgtctac ccgggccgct tccgccctac ctagggcgtg
      841 gcgtaagcag tttggcaaga tcaaaggcga aatgctggcc gcattcgtgg ggttcctgag
      901 ggcacgattc gtcctccttt cgattactgc cgtcatgacg attctcggcc tttcggtgat
      961 gcgagtgaac tatgcgtggc ttctcgggtt agcctgcggg gcgctggaca tacttccact
     1021 tgtgggtccg tcggccctgt ttcttccctg ggcattttac catatactgg tggggaacat
     1081 cggattcgcg tttggccttg ttgccgtgct tgcactgagt tcggctgtaa ggcagatcgc
     1141 tgaagccagg gtgatgggca ggaacctcga cctgcatcct ctagtggctt tgatctcagt
     1201 gtacgtgggt gtgcaactgt tcggcgtaaa ggggttcata gccggtcccc tggcagtgat
     1261 attcttcaag gcgattctgc attctatcat tctgccggtg tttccccttg acaaggagta
     1321 agcgatgcta tcagtctgca ttctgggttc taccggttcg gtaggcagtc aagcaatcga
     1381 ggtggctagg tcgctgagcc ttccggtgac cgggctttca acgtggtcca atgttcgtct
     1441 cctggctgag caggcgcgcg agttcaggcc acgagcgtgt gcagtgggcg acccccgcat
     1501 ggcgggcgat gcccggaacc tgctgcgcga gctgccggga gctccgcagc tattcgtcgg
     1561 aaccgagggt ctttcggagc tcgcctccat ggcagagcac gaaatagtgc tcaatgcagt
     1621 agttggtttt tcgggtgtgt atccgaccct cgcagcgatc gaagcagggc atgacgtagc
     1681 gctggcgaac aaagagacac tggtggcagc aggcagtatc gtaatggagc gcgcgaggcg
     1741 gcgaggtgta tcgataaggc cggtagacag tgagcattct gctatctggc agtgccttgc
     1801 cggtcaggac aggcagagcc tttctcgcct catactgacg gcgtccggag gacccttccg
     1861 cgacaaggtg gatctttccg atgtaactcg agaacaagcg cttgctcatc ctaactggag
     1921 gatggggccg aagatatcgg tggattccgc cacgatgatc aacaaggctc tggaggtcgt
     1981 agaagcccac tggcttttcg atgcctcgcc ggatatcata gagattgctg tgcaccgcga
     2041 gagcatcgta cattccatgg tagagttcgt cgatggatcg gtcattgccc agatgggcgc
     2101 ggccgacatg cgcactccga tacagtatgc tctgacgtat cccaaaaggt gtgaagggct
     2161 agctcagaag ctcgatttcg ccgaacttgg aaccctcact ttcgcggcgc ccgacgccgc
     2221 gcgcttccca ggtgtcctgc ttgggcatca agcgctgcag ctcggaggag tagcgcctgc
     2281 agccatgtct gcggcaaacg aggcagccgt agaccgattt ctccgaggca cgctgcgctt
     2341 ttgcgacata gctcccgtcg tggccgaagc catgcacagg agcgtagatg gtggggaact
     2401 ctcgcttcgg ggaatcgttg aagcagatgg gcaggccagg cagtttgtag aggagaggtg
     2461 cggcggatgt acgcgctcgc tttcatagta gtagtcggtc tagtggtact cgtccacgag
     2521 ctgggccatt tcgcggcagc caggcttgct ggagttcgag tgtacgaatt cgccatcggg
     2581 ttcggccctc gtctgtttgc gaagcagata ggccaaacgc tctacgccgt gcgcgcgttg
     2641 ccgcttgggg gcatgtgcag gctggcgggg atggacgact cggaacttga gagcgaacag
     2701 actgatcccg acgacccggc gagcttcgtg aaccggcctt tctggcagag gatactggtg
     2761 gttgcggccg gtccgctgat gaatttcgta ctggcgatcg ccatcttcac cgggctgttc
     2821 tcggcatatg gagtgccgat ggccagagtc gtggatgtca tggaagcaag ccccgcgcag
     2881 gtcgcgggct tccgagcggg cgatacgatc ataagcataa atggaaggcc ggtagcatcg
     2941 gttgccgggt ttgtgaacac gatatcgacc agctggggaa ggcgcctgga tgtggagatc
     3001 cagcgggacg gacagagaga cacagtgcac gtggttcccg agttcgatgc tagagccggg
     3061 gtgggtcgca ttggcgtgag ccttacggag tcgcccgtaa aatccgaggt ccgcggtact
     3121 gtgggcggcg cgatcaggta cacgtgggga gtgatcgata cgacgatcac ctctttgtgg
     3181 gcggcgctgt cgggcagggc taagcctgag gtgtcaggcc cgctaggcat cgcaggcatg
     3241 tcggctcaag ctgcgcagca gggcctgccc agtctcctga tgttcatcgc cctcatctca
     3301 actagcctgg ggcttttgaa ccttatgccg gtgccaatac tagatggagg ctggattgtc
     3361 cttttggccg tggaagcggt gcgcggaaag aagctctcac cccaagtaga agaggcgctc
     3421 aggttcgtcg ggcttgcgct cctgattctg ctcatggtgt acgctacagt gagcgatatt
     3481 tcgcggctga tcgtccgcga gatgtaattc tttgcggcat gtggatggtg tgcgggattg
     3541 actactcgcg ataacacccg gcaagtgctg gccggacggg ttcccatggg cggaggcgct
     3601 ccaatatcgg ttcaatcgat gactaatacg gacacacgcg acgcagtggc gactgctgcc
     3661 cagatcggca ggctacaaga ggcgggatgc gaggtagttc gtattgccgt gcccgatatg
     3721 gaggctgcgg acgccctggg caggattcgc aagaccaccg aagtcccgtt ggtggccgac
     3781 atacacttcg actataagct cgcattggcc agtatagagg cgggcgtcga caagctgcgt
     3841 atcaacccgg gcaatatcgg cagcgatgcg cgcgtgcggg aactagcgac agccgctcgc
     3901 gataggggca ttccgatacg tatcggagtc aacatggggt ctctccctcg cgagcttgta
     3961 gagcgccacg gccgcagcgc gcgcgccatg acagaagcgg ccctgggcca catcggcctg
     4021 ctcgagcggg aagggtttta cgacatagtt gtgtcactca aggcgtcgga tgtgcctcgc
     4081 acggtagaag cgtaccgtat gttgtcggcg cgagtagatt atcctttaca tgtgggggta
     4141 actgaagcag gcccccctcc cagcggcctt gtgaagtcgg cggtggggat aggcatgatt
     4201 cttggagagg gtatcggcga caccataaga gtgagtctga ccggcgatcc tgtttccgag
     4261 gtgtcagccg gccgcgaaat cctggccgca ctggagctca ggtcattcgg gcccaccata
     4321 gtctcctgcc ccacatgcgg cagacgacgc ggtgatgtgg tgggaattgt gtcggccgtg
     4381 cgcgaggcgg tatgcgatat tgctgtgccc gtgaccgtcg cggtgatggg ttgtgaagtc
     4441 aacggccccg gtgaggcgca ggatgctgat gtaggactgg cgtgcgggcg cggagtcggg
     4501 ctgatattca tgaacggacg cgtagtgcgc cgggtgccgg aggaagaaat ggtgcctgcg
     4561 ctggttgaag ccgtaaggat ggttgcttcg gccaaagaga taacttgtcg cgctcaacgc
     4621 gaggccagag ggggggatga ccatcatgcg cgtctcggcg atagtgcctg catacaacga
     4681 aggccctaga atcgccgccg tgctggatgt cgttcgccag agcaagatgg tggacgagat
     4741 actagtggtg gatgatggct cagatgatga tactgcagaa gtcgcggctt tgcacggagc
     4801 gaaagtggta cgcatgggtc acaatttcgg caaaggagga gcggtagcgc gtggaataca
     4861 gtccacattg ggcgatgtgc ttctgttcct cgacgcagat ctggtgggcc tcaccgtcgc
     4921 tcatgttgaa ggcttacttg tgccggtgct atccgataag gctgacatga ctgtgggcat
     4981 cttcggagat gggcgccccg cgactgatct ggcacaggcg ataactccgt ggctatcagg
     5041 tcagcgtgcc gtgagacgca gcgcatttga cggcctggac ctgtgcgaat ccgggttcgg
     5101 cattgaagcg ctgcttacgc ggcatgctag ggagtcgggg atgagagtag tggaggtcaa
     5161 gcttcccagg ctcacccagg tgatgaagga ggagaaactg ggcccggtga agggcgcggc
     5221 cctccgggtc aagatgtatt gggacgtcct gaagactgtc gttcaccgag attaaggttt
     5281 caggcagtag ctggagggtc ggttttgtct acattgctag tgatggttcc gtccgacgac
     5341 cgggcggagc ttggacatat attcgaacgt gccggtatga gcgctcccga gttcctgtcg
     5401 ccttcctgca agatagagaa ggtcgacgtc tacccccagt caaggcaatg ggttgttcat
     5461 ctggttggcg atggcgacct cagcgaggag tgctgcgagc aggttcgcct ggcttttcga
     5521 aagattctgg gagatcgctg cgatgtgagc ataaggctgt cggctgagaa cgctggcggt
     5581 atcgacagcc acacactgga gggctgctgg gaacaggttg cccggcgtgt gctcgatcgc
     5641 gcccccagtg tgggggtgtg gctaggccag gccagatgtc gcgtggtcga cgaccgagta
     5701 gtcgtcgaag ttccgggaga ggtgcagcgc accaagctgg cggagcgagg atgcgcggac
     5761 ctgatctcgg acgctctgcg tgatatcgtc ggcgtccggg cgtcagtatc gatagacgtg
     5821 ggcgagttcg atgcacccga ggttccagat gacgatgtat gcatgcagcc ggacgcggat
     5881 gcgcgcgccg ccttgggcag tcgacctacg cctcctgttc agccatctcc gactggggct
     5941 gagagacgtg gcggcaggcg gcgcagggta gttaccgacg aaggcgccgt tcgcgggcgc
     6001 cggttcaccg acgcccccca acccctatcc agtctggttc agggtcagaa gaaggccgtg
     6061 gccagcggcg aagtatttgc cgtcgaagag aggctgaccc gcaccggcct cagaatcgta
     6121 agtttctgca tcactgatag gcaggattcc atcgcctgca agtgcttctg tgacccggaa
     6181 gagccggttt ttgaactctc ggaaggtcag tgggcgcgcg tgagaggaga tgtgcagtac
     6241 gaccagtacg cgcgagagat agtgcttgcg gtgtcggaca tcatgcccga ctctaggcct
     6301 gcgcgatccg atactgcgcc ggagaagcgt gtcgagctcc acctgcatac caagatgagc
     6361 gccatggact ccgtatgcga cgtcgaatcc gtaatccggc aggccgcagc atggggccac
     6421 ccggcattgg caataacgga tcacggcgtc gtccagtcgt ttccagacgc attcgcagcc
     6481 ggcaagaaac acggcatcaa gatcatatac ggcatggaag gctatctcgt ggatgacgcc
     6541 gcaaccgatg actcccctac gtgccacgtc acgattctgg cgaagaacgc tgccggcctg
     6601 aaagacctct acatgctggt gtcggcatct caccttcact acttctaccg gcatccacga
     6661 ataccgcggg cactgcttgc gaaagcgcgc tcgaaccttc tcatcggttc tgcatgcgcc
     6721 gctggcgagc tgtatcgcgc ggtgctcggc ggcgcctccg atgaccaact cgaccatatt
     6781 gcgaagttct acgactactt ggagatcatg ccggccggca atgacgagtt cctgattcgc
     6841 tcgggcatgc tgcgcggaat cgaccaggtg caggcgattg cggctcggat atacggtatt
     6901 ggcaagcgtc tggggattct ggtggccgcc acgggcgatg ttcatttcct tgagccggca
     6961 gatgaggcat atcgtcgtgt ccttatggcg gggcaggggt atgaagatgc cgatcatcag
     7021 gcgcctctgt actaccgcac cacagatgag atgcttcgcg aattcgcttt cctcggagag
     7081 cgggcgagcc gagaggtcgt tatcgacaac ccaggaatca tcgctacaca ggttgagtgc
     7141 atgcggccgg tacccggcga gttggccaca ccccagatcg aaggggccga cgaggaggtg
     7201 cgtacgcgaa cctatacgaa ggcccgcgag ctatatggag atccgcttcc ggaagcggtt
     7261 aggtcacgcc tagagcgcga attgacgtcg atcatcggca acggtttcgc tgtcaactac
     7321 gacattgcgg ctaagttggt gcacaaatcg gtcgaggacg gatatccagt gggttccaga
     7381 ggatcggtcg ggtcctctct tgttgcgacc atgtgcggca taaccgaggt taaccccctg
     7441 cctcctcatt acctatgtag caactgccgg tatagcgact tcgatcatgg tgtgcaggtg
     7501 gagtcggggt acgacttgcc ggacagccag tgccctcgtt gcggctcaag gctgggcaag
     7561 gaggggcaga gcatcccctt tgaaaccttc ctgggcttcg aaggcgacaa ggttcccgac
     7621 atcgacctca atttctccgg gcagtaccag tcaacgatcc acaagtacgc tgaggagctg
     7681 ttcggggccg accacgtgtt tcgagcgggt actatcgcca ctgtagccga caaaacagcg
     7741 tacggctttg tccgggcata cgcagaatcg cacaggctca ccttgcgcaa tgccgagata
     7801 tctaggcttg cccgagggtg ttccggcgtc aagaggacca cgggacagca cccgggcggc
     7861 gtgatgattg tgccgaaggg cagggatgtc catgagttca cacccgtgca gcatccggcc
     7921 aacgacaggc agtccgaagt cgtgaccacg catttcgagt acaagacgat tcacgactgc
     7981 ctgctcaaac tcgacctgct cggccatgac gacccgacaa tgatcaagat gctggaggac
     8041 ttgaccggca tatctgccgg cgacataccc atggacgatc cggctacgat gtcgatcttc
     8101 tccggagtcg aggcgctcgg ccttgaccct gaggaaagcg ggatgaccgt aggtaccgta
     8161 gctattccgg aattcggcac caagttcgtg cgggggatgc tggaggcgac caggccaaag
     8221 tctttcggtg aactcgtacg catcagcggc ttgtctcatg gaaccgacgt gtggctcaac
     8281 aacgcgcaga ctttgatcga atcgggcgct gcgcggctga ccgatgtaat tgcctgccgg
     8341 gacgacatta tgaacaggct gatcttcgac gggctagctc caaaagacgc tttcaggatt
     8401 acggagcgcg tgaggaaggg aaagggactg gccgatggtg acgatcagct aatgcgtgat
     8461 cacggtgtac ccgagtggta catcgattcc tgcaacaaga taaagtacat gttccccaag
     8521 gcacatgcgg cggcttacgt aaccatgtcg tttcgcattg cctacttcaa ggtacatcat
     8581 cccatcgagt tctacgcgtg ctatttcacc ataagggcct ccgacttcga ctcctccatc
     8641 attgccatga gcgtgcagga tatcatggag gctatgaaag caaccgatgg aaatcaagac
     8701 gctaccgggc gcgagaagag catggcggcc atcatggagg ctgtggcgga agccagaatg
     8761 cgcggcgtgt cattccttcc tgtggacatt gccaagtcct cggctacgag tttcaccatc
     8821 gagaacggat ccatcaggct cccgctggcg agcgtggctt cgctaggaga tactgcggcc
     8881 tcaagcgttg tgcaggcgcg tgaagaacgg ccgttcagct ccatacagga cttgcgcgat
     8941 cgaacgcgcc tcacgcggtc gcatgttgat acccttcgcg atatcggagc cctggcaggt
     9001 ctgcccgaga cagatcagct atcgctcttc tagcttttgt cggcccttgc aagccgcatc
     9061 agcctgtggt atattgaaaa ctgtgaggat agctggcggc aggcgcgtgc ggagagtgtt
     9121 ttcggagagt gggtcggtcc cactctttaa tcttgtctga ccgggaaagg gccaaagccc
     9181 tgcatgttcc ggctaagtaa gtaggagggc tgagctggat ggatcgtcgg cggatcgaga
     9241 tccgcgcaag gagtattgct gagtcagccg ccgggccgct cggcctgaac gtagtcgatg
     9301 ccgaattcgt tcgcgactct ggctactggt atctgcgagt gtacatagac aaaccaggcg
     9361 ggatagccat cgacgattgt gccgatttgt ccactgcgat cggggccgaa ctggaccggc
     9421 aggacttcat cccggagagc tacattctgg aggtgtcgtc ttctggggaa aaaccacttc
     9481 gccgcgacga ggaatacgat gagttcaagg gcaggtgggc cctgatcaac acatatacgg
     9541 agatcaacgg atgcaaacgc ttcgaggggc ggcttatggg catggtgggc gattcggttt
     9601 cgatgctggt cgacggcaag gctgtcgaga taccgcgacg caggatcagc gcagccaggc
     9661 tggcggttcc tctcagggag gtgtgcggtg atgagtcaga atgatggcct cgagatagtc
     9721 cgggcgctgg atgaactcca gaaggaacgt gggatagcca aagaagtgct gctggatgcg
     9781 attgaggcgg cgctcgtttc ggcgttcaag aggcactatg gctcgtcgca gaatgtaagg
     9841 gtaatgattg accccggcac cggcaaggta ctagtgctgg cacgcaaggt tgtcgcggag
     9901 gaagtagagg accccaaaac gcagatttct ctggatgatg cccgagcgat tgatccgggc
     9961 tacaacccgg gcgatgtgat cgaaaatgag gtcacgccca gggaatttgg gcgcatagca
    10021 gcgcagactg cgaagcaagt tgtggtgcag cgcatccgcg aggccgagcg gggcgttcta
    10081 tatgagcagt tctccaaccg tgaaggcgac gtcgttaccg gtacggtcag tcgagtggag
    10141 gccagaaacg tcattctgga cctaggcaag actgaagcct cgctgcctca gcaggaacag
    10201 gtcccaacgg aacgatacga gccgggcatg aggctgaaat gctacgtagt agaagtccgc
    10261 aagaccaacc gcggaccaat ggtggtgctg tccagaacgc atccggggtt gctaaagaga
    10321 ttgctggagc tcgagatccc cgagatttct gaaggcgtcg tggagatacg cggcatagct
    10381 agggaggcgg gcgccaggtc caaagtcgca gtgctcgccc atgacccgaa cgtggacccc
    10441 gtaggggcct gtgtgggtcc gcggggcgcc cgcatccagg cagtggtatc ggagctagcc
    10501 ggcgagaaga tcgatgtgat accgtggagt gaggattact caacattcat cgcgaatgcg
    10561 ctgagcccgg ccaaagtcgt ggaagtaagg cccgacttta tctcaaaggt cgcgatggta
    10621 gtcgttcccg attaccagct gtcgctcgcg ataggcaggg aagggcagaa cgcgcgacta
    10681 gctgcgaaga tcaccggctg gaagatcgac atcaagagcg aggcccaagt agggctgggc
    10741 ggaatacctc ggttcgaaat cgacttctag aggtgcgtga ggatgcctaa ggtcagaaag
    10801 acgccacagc gcacctgtgt gggctgcgga aaggtgacag acaagcgcag tctggtgcgc
    10861 atagtgcgga gtccggaagg cgatgtggtt gttgacccca ccggcaaacg gtcggggcgg
    10921 ggagcgtacg tgtgtgcggc acgcgagtgt ctgcagcggg cgctgcgtac gggcaagctt
    10981 tcgaaagcgc ttgagacggc agtgccggaa gatgtagtag cagcgctttt gggcagtttg
    11041 ggcggagttg atgaatcgta ggtgtcttcg ggttttcttg ggaccttggc catgcgtatt
    11101 cggccaggac tatcgccgag cgtttgcgcg cgccggcgct tcacccaggg gcctatgaag
    11161 cgccgcagga tagttcggcc ttatgcgggt catgggggtg acgatcaatg gctaaagtga
    11221 gagtattcga gctggccaaa gaacttgata tgcctagcaa ggacctggta acgctcctaa
    11281 atgagctggg cgtgaaggcg aagaaccaca tgagtgcgat cgacgagaac gcggcgcgct
    11341 atgtgatgag gaagtacgga cctgacgcga agaaggcgga atcgccgggc gagcagccaa
    11401 cccaggacgt atcagaggcg cctaccgcgc ctccggctga tctgccgcgc gctgctgtta
    11461 gggcagaacc ggtgcggggt ccggccgatc gtcgggttga cgcaggtccg caggacaggc
    11521 cacgcccagc gccggcgacc cgcccccaag acgccgggcg caggcagggg cgaccacagg
    11581 gcgtaccagg cggccagacc cggccagcgg atgcaggagg ccgtccacgg cgaccgctag
    11641 atgtcgggcc tcaaaagcct cttgacccgt catacccatt tatacctgca aggccaaggc
    11701 cgcaaggcgc aggcgcgccg cgtccgcgtc cttttgcgcc tgttacgcct attacgcctg
    11761 ttgcgccggc accgcctcag caggcacttc aacagcccag gcctgagcgg agtgataggt
    11821 tcgaccgggc cgagcgtttt ccaaggccgg cagggcctgg gccgggtttc cgacgtgggc
    11881 cggctgtgtc ggttccgccg ccgccaccgt ctgctgttcc agctcccggc gccagaggcg
    11941 gcggaaaggg gaaacagacg tggtcaggtc gcgggaaaag gcgaggcggc ctcgatgacg
    12001 agatggaagc ccccaggggc gaccgcaggg ccgaccgtcg tggccgaaag aaatccccaa
    12061 ccgccgcacc attgggcgat gcgcggctga ttgaacgccg cgtcagggct ggcgatatcg
    12121 atgcggctga tgacgtagtc gttagaaggg tcgggcggag gcgcgagaag cccaccaagg
    12181 tcaggcggat tgagataggc gcatctatag gaatccgcga tcttgcagct cgattggaga
    12241 tgtctgcgcc ggaagtggtg agcaggctca tcaagatggg cgtaatggcc aacgtcaacc
    12301 aggagatcga gggagagatt gcgcagatat tggcgaccga tctcggtttc gaggcggaac
    12361 tcacttccga acgtgaagag cgtgaagtgg ccatcgccga aatcgaagat gctcctgaga
    12421 gcctgaaatc caggccgccc gttgtcacga taatggggca cgtagatcac ggcaagacgt
    12481 ctctcctgga cgcaatacga gagacgaacg tgacggccac agaggcaggc ggcattaccc
    12541 agcatatagg ggcttatcag gtggaattgg gcggcaggaa gataaccttc ctggatacac
    12601 ccggccatga ggcctttacg gcaatgcgtg ccaggggagc gcaggccact gacgtggcgg
    12661 ttcttgtggt ggcggcggat gacggcgtga tgccccagac cgttgaggcc atcaaccatg
    12721 ctcgcgatgc caacgtgccg attgtggtgg cggtgaacaa ggtcgacaaa ccgcacgctc
    12781 agcctgagcg cgtacagagg caactatccg agatggggtt ggtgcctgaa gaatggggcg
    12841 gcgagactgt gatggttcca gtgtcggccc tgcagaagcg agggatccag gagcttctgg
    12901 agatgatcct gctcgtagcg gatatgcgcg atctcaaggc gaatccggat cggccggcca
    12961 tgggtactgt cattgaggcc aaagtggatc gtgggcgcgg accggtagcg acagtgctca
    13021 ttcagaaagg cacactccgg gtgggcgact ccttcctagt cggcgaggtg tacggtaggg
    13081 taagggcgat ggcgaactac aggggagaac ctctggacga ggccacccct tcaatgccag
    13141 tggaagtcct tggcttaagc tacgtacctg aggccggcga tgtgttccag gcggtgacag
    13201 acgagcgcga ggccaagcag gttgcggaaa agcgggccga gcggaagcgt gtcagcgaga
    13261 tcggggagac cagcaagaag gtatcgctgg aggatctgtt cgagcagatt aaggagggcc
    13321 agaccaaaga gctcagggtc atcatcaagg ccgatgttca gggctcggcc gaggctttga
    13381 aacaatcgat tgagcagttg tctaccgatg aggtgcgtgt gaacgtgatt cacagcggcg
    13441 tcggcgcggt cactgaatcc gatgtgatgc tggcgtctgc gtccaatgcg gtagtcatcg
    13501 ggttcaacgt tcgacccgac gcggccggca cggccagcgc cgaggtggag caggtcgatg
    13561 tgcggctcta ccgcgtgata tacgaagccc tcgacgacgt gaagaaagcc atggaagggc
    13621 tgctcgaacc ggagtatcgg gaagttgttt tgggccatgc tgaggtgagg gcgaccttca
    13681 agattcccaa ggcaggtact gtggccggct gctatgtgac agacggcaag atagcaaggt
    13741 cagcccgcgt gcggctcttg cgcgatggca ccgtgataca cgaaggcaag atctcctcgc
    13801 tcaagcgatt caaggatgat gcgcgcgagg tggctgccgg gttcgagtgc ggcattggaa
    13861 tcgaaggcta cagcgatgtt cgcacaggcg acatcatcga aggatacagc aaggaagcgg
    13921 tcaaacgcga gctgtagagg cgggtgatcg cccgtgatta tcggaaggat gcattgccgg
    13981 ctggcgatag acggcagcca ttccctcaag gacaagaagc ggacgctcac aagccttctc
    14041 gcccgtgcga gaaacacgtt taacgtggct gtagcagagg tggacgacct tgagatgtgg
    14101 aatcgggctg ggataggggt tgcgtgtgtg tcaaatgact ccggccatgc ccatagcatc
    14161 ctcgagaagg tggcgcgcat gtttgattcg tgccccgagg ttacacttga atcatacgag
    14221 atcgagatcg cgtaatcgtt cagccgccga tctcgaacgc tgggaggtgc tgctgttgtc
    14281 ttcccgccgg aatgaccggg tgcgggctgc aatgcgccag gaaatatcga acatgcttca
    14341 gcgcgatctg aaggacccta gactgggttt tgccacagtc actgatgtcc gggtcagcgg
    14401 cgacctccag catgtgcagg tgtacgtgag catcctcggc gacgacgctt caaggcagca
    14461 gacgatggaa gctctggaga gcgcgcgagg atatgtgcgc tcggagatcg ggcgccgtgt
    14521 gcaactgagg ttgacgcctg agatcacctt tgcctacgac gactccattg agcgtggcgc
    14581 ccgacttctg cgactcatct cggaggtcca aagcgaggag acggctgccc ggcgcgatga
    14641 ggagaatgac aattgacggt gcccggcgag atcattgagg ccctgggaag tgcccgctcc
    14701 gtcttcatta ctacccatgt agttcccgac ggcgatgcca tcggctcgct cctgggcatg
    14761 aaactcgctc tggagcgcct cggcaagcag tgtgaggccg gtctatccga cccggtgcct
    14821 ccgtcgctgc gcttcttgcc cgggtcggcc ggcgttcggt cgggcggatg tgaggcgacc
    14881 ggtcggtacg acctggctct cgtgctcgat tgtggttcgc tggaccgtgt cggtcaatgc
    14941 gtagagaccg tggaagcttg cgcggtcagt gtgaacatcg accatcacgc cacaaatacg
    15001 cggttcgccg cggtcaactg ggtcgatacc gaggccgcag ccacctgcca gctggttgcg
    15061 gagctgattt cggccatggg cgtcgcatat gattcggata ttgccacctg cctgttcgcc
    15121 ggtttgtcca ccgacacagg ctcgtttcgc tactccaaca cgcgcgcgtc gacgttccgc
    15181 atcgctgcgg agttggccga cgcgggcgcc cgtccgtggg agatcgccca gaacgtctac
    15241 gacagcaggc cctacaattc cctcgcggcg ctggccgagg ccatacttgg catcgaattc
    15301 agctgcgacc ggctaattgc gtcgatggtg atcaccgctg ccgatatggc caggcacggt
    15361 attactgaag gcgacaccga agggttcata ggctacgcgc gatctatcga cggtgtggag
    15421 attgctgtag ccttacggga agtggggccg ggaaccatac gcgtggggtt gcgctcaaaa
    15481 gagcgagtgg acgtcgccgg tgtggccggc gaattcggcg gcggaggaca cgtgcgagct
    15541 tccggctgta ccattcaggg aaccatggaa gaagcccgaa ggaagctcat ccccagtttg
    15601 gagcgggcgc ttcgcgaggc gggatacacg tggacggcct gatcgccata gataagccgc
    15661 ccggcatgac atcacacgat gtcgtggcgc gtgtgcggag aatcgcgggc cagcgctctg
    15721 ccggccatac gggcactctc gatcccgacg ctaccggtct tctcgtggta tgcatgggca
    15781 gagccaccag gctcacacgc ttcatggagg gattccccaa ggagtatgaa gccgaaatcg
    15841 tgttcggagt gtccaccgac accggtgacg acagtgggcg cataacggca cgcagcgaga
    15901 gcttctcgat ccccctttcc gagttcctgg atacgctcgg ccggttccta ggcgatatcc
    15961 ggcagacgcc gcccatgatt tcagccgtgc accacgaggg cgaacgtctg tacgaactcg
    16021 cgcgccgagg catcaccgta gagcggcaac cgcgcacagt gaccatattc gagatcgaac
    16081 cgttgtccgt cgtgagttgg ccttcgcgcc tggagcatgg ggcacgcgga aagctcagga
    16141 ttcggtgctc gcgtggaaca tacatccgaa ctctctgcga agacatatgc cggagcctgg
    16201 ggattgccgg gcacatgggg gccctgacgc gaactgcgac atcagggttc actctggatc
    16261 aggccgtaac ccttgaagag ctcgaacacc ttgcctccct gggatcgctc gccgaggtaa
    16321 tcaagcctcc tatagaaggt gttaggcacc ttccgtcgct cgatcttgcg gagcgcgaag
    16381 cagacgcggt cagtcatggg atggccttcg agtgcgatgg aaatcgcatc atgaatgcac
    16441 cggaggatct cgtccatgtt gctctggtgc gccccgacgg aaatcttgcg gccgtagccg
    16501 ccgcgagtcg ctctgagttg gggcttttca tccagcctag agtcgtactg tgagtcctga
    16561 ggtgttgtag aatgcatcga tgcttgataa gccgtaatca acgtcccgaa cacgttgcaa
    16621 aggtgtacgc cctgggtatg ttcgacggag tgcacatcgg ccatatggcc ctgattcaga
    16681 cggcagtaag gatcgccggg gaacgcggca cgacgtgcgg cgtgtacacc ttcgacgtcc
    16741 accccatgcg tgtggtgtgc cccgagcgcg cgcctgacgc ccttatgtct cttgacgcca
    16801 ggcttgacct gatggagtct gccggagtgg acacggcggt agtccacacc ttcgatacgc
    16861 agcttgcgag catgcctccc cacgcctttg tatcccgtgt tcttcacgac gcgctcggcg
    16921 cccagtgtgt ggttgtgggg tatgattact cctttgggca gggtggggca gggacccctg
    16981 ccaccctgga actgctagca agggaagccg gcatatgtgt gcagacgctg cctccggtcc
    17041 ggatcgacgg tgcaccggtg tcgagtacgg gcgtgcgaaa ggccctggcc tgcggggatg
    17101 tggaacgtgc tgctttgctc ctgggcaggc ggcatttcgt acggggaacc gtggcgaggg
    17161 gacgaggagt cggccgcgag cttgggatac caactgcgaa tgtgagcgtg ccggcaggga
    17221 tagctcttcc catgccgggc gtgtatgcgt gcgaggcgcg ctgggggtcg tcggcgaccg
    17281 ttccggcggt ctgcaacata ggagtgaggc ccaccttcga cgatcagggg cggggagtca
    17341 cgctagaggt acacgtgatg ggcgtcgcac ccgacctgta cggggcgttg ctgacggtgg
    17401 agttcgcagc aaggctcagg gatgagctga ggttcgccag cccttccgag ttggtaaggc
    17461 agatcgaggc ggacatgatc gccgcccgac gccttctgga gccttgagtt tacacctggt
    17521 ggcgcgtatg ctagaatgaa tcccggcaag ctgctacaga aatgtttgct gaaccctcta
    17581 ctagggctgc cgaatctccg acggctttcg tggtagtggg ggatccaaaa tgaggaggtg
    17641 ccgagtcctt tgagcctgag cattgaagaa aagcgagcca tcatcgagaa gtatcgagtc
    17701 cacgattccg ataccgggtc accggaagtc caggtggcaa tcttgacgga aaggataaac
    17761 taccttacgg agcacc
//
LOCUS       DAOTAC010000010        17489 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_2691_length_17489_cov_4.662212, whole genome shotgun sequence.
ACCESSION   DAOTAC010000010 DAOTAC010000000
VERSION     DAOTAC010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 17489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17489
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2691_length_17489_cov_4.662212"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="PLM74_00905"
     CDS             <1..370
                     /locus_tag="PLM74_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HON41675.1"
                     /translation="EPVERIVTHTAGAHRCQASVTVNGPHPAVINDAALSALAARSVQ
                     TALGPGYLAACEPWMASETMGCYLSKYPGVLALLGIACDKVGAGAPHHSPSFDIDESA
                     LKYGVAATLQFARDVLLQSP"
     gene            complement(367..948)
                     /locus_tag="PLM74_00910"
     CDS             complement(367..948)
                     /locus_tag="PLM74_00910"
                     /inference="COORDINATES: protein motif:HMM:NF024231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HON41676.1"
                     /translation="MKRDSKTELRRLSTMAVLTATSIVLFLVFRTPIFPAVPFLVYEA
                     ADVGLLVAGFALGPWAGLACTAVVCVLQAPLHPEGGWFGAVMHLISSGALVGVSSIIY
                     SRFRSRAGAFWALLAGTAAMVGVMIPANLILTPRFYHIPTSAVVSLLGWIIAFNAVKA
                     GINAAITAAIYKPLSRLIKGASPENAVAPAQMH"
     regulatory      complement(1034..1184)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1390..2355
                     /locus_tag="PLM74_00915"
     CDS             1390..2355
                     /locus_tag="PLM74_00915"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HON41677.1"
                     /translation="MSDTGKNRRRRAAWVLVAIAALALIIWGAIRGRNKSLVEARLPA
                     ATAGLATTSVDQGDVNEYVSCTGSLMPIRMQVMASDASGGVTRVYVKEGARVARGAAL
                     CEVQRSSAFDFSSQSDKTRLTAPFDGVVSSVTVQAGGYVGAGQPAVTIMDTSSYFASA
                     EIDEIDLARVEPGMAAIMSFDALPDVELPGKVEWIGVAALPRAGMVVIPIRVSIDGSH
                     DLLKPGLTTNVRILSRSIQGVVRISVRSWVDMDGQPSAIRIVGGAPKLIPVELGLSDG
                     NYTHVLSGLSPGDEIVEDAVAAQEKLRRLTGQDRDMQFRIRHADQ"
     gene            2409..3095
                     /locus_tag="PLM74_00920"
     CDS             2409..3095
                     /locus_tag="PLM74_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011405550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON41678.1"
                     /translation="MGSVEVHALDGVSFDISDGDYVSIMGPSGSGKSTLMHIVGCLDR
                     PSSGVYRLHGDDVSQLDDTELARIRNRRIGFVFQQFNLLPRETVLGNVEIPLMYAGVG
                     GRDRRRIAGEALARVGLADRMHHRPAEISGGQKQRVAIARALVNDPSLILADEPTGNL
                     DSKTGGEILAILDGLNAEGCTIVLVTHDRSVAERARRIVQLRDGSVERIEELGIEQPA
                     ERGSRCDGHS"
     gene            3082..3681
                     /locus_tag="PLM74_00925"
     CDS             3082..3681
                     /locus_tag="PLM74_00925"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HON41679.1"
                     /translation="MGIAETIGVALRNLWANKLRTALSVLGIVIGVASVITMISAGSS
                     AQKQITSDISALGSNMIMVTPNLKPGMQGRSHLHLLTMSLADQIQAAAPAISAVSPVA
                     QMEGRIIAGSASASAVITGITPAYTRIVSTRIALGRSLIQSEVNAAAHSIVLGSEVAS
                     DLFGPDDPIGRQVALVSGDRRAVFTVVGVTESKGGTVLG"
     gene            3707..4288
                     /locus_tag="PLM74_00930"
     CDS             3707..4288
                     /locus_tag="PLM74_00930"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HON41680.1"
                     /translation="MTTLLYRMSPIKRVSTYFCQAASAQATDVAVAQVERFLASVAGT
                     PDAFSVASQAGLIDAIKQVTATMTLLLGTIAGISLVVGGIGIMNTLLTSVAERTREIG
                     VRKSLGACNRDILLQFLSEALVLSMLGGTVGMLLGWLGGAGIARIAGWPVSVDLSSVI
                     AAVSFSTAVGLIFGTYPALQASRMDPVQALRYE"
     gene            4317..4847
                     /locus_tag="PLM74_00935"
     CDS             4317..4847
                     /locus_tag="PLM74_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41681.1"
                     /translation="MASTIRGRVGGVIGGYSVSCITTPTSVSGRVGGAVIGGDLNLDI
                     EPPPGRIFGRVGGAIIGRTVDARFADSSGNSVHGRMGGIIDGVDITLSGTDRITGRCG
                     GPVIGFDVDLEVADGQMTGRLGGAVVGHSVSFSMAAGLPNVVTALVAVIAYQYYLIAQ
                     RKSSSSSSSSSGSGGN"
     gene            complement(4977..6164)
                     /locus_tag="PLM74_00940"
     CDS             complement(4977..6164)
                     /locus_tag="PLM74_00940"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HON41682.1"
                     /translation="MKVRRIAALLLLLLVVVATTTGCGGPGLITGAIDGYVWKHADAR
                     SAQAEGEPGFGVMAEAPKGNLAPLAGAYVVAEGPNGSAIARSDSNGYFRIGRLSSGYY
                     KVTITHEKYLDVYEKSCYAEAGGTTRIGGTPMLGSLHILAIGINDYQSGYISDLSYAV
                     ADAELIVTRLGVENRLAKQYTLLTAEWDTTKSSIQNAIRDIGYNITNGDTFIMFYSGH
                     GTHNDAGTTEYIVPSDANPLNLGTMISDQDLNNWINQYMPAYAKKIFIFDSCNSGGMY
                     RSLAALPKGFTWSTGFEVMARNITGPRKIIMTACAKDENSWESSRPPIGGHGVFTWAL
                     TEGMRYPYPADADLPRPNHAIDTEEAFNYASHWTKYYSPGTQNPEIYRGESGSEWWYL
                     FTY"
     gene            6401..7654
                     /locus_tag="PLM74_00945"
     CDS             6401..7654
                     /locus_tag="PLM74_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41683.1"
                     /translation="MLRTRLAPACCIALLLCLINIAPGMAIAPGTTGFHSPVGSRGPS
                     LLDRSAEAGSAVDLMPEAKSLQAPSWVREGLRVYYRTSSATIENPFAAASNAPKPPVV
                     RVDQVSTSAPAVVRTDVIALAPRFSATYSTIFIGDLMIPWDDSSSSGFAGIGEFWINP
                     AALRDAAKRSSSGLSAEPVHFEVGGKAYSALRLDSTQANPTRYLTWVFDTSTGLLLYQ
                     AQWMALADRGLAHLSVAEFVSMRNMTLPWDGGVTPPWVKQGTRLTYQGSIQTWTPAGG
                     RNIPIPAVLEVEMTSVGRNWSAARVKRVFSSDGEDQAITVYSGTGLLAGGFWVPRANL
                     ARLRSGERLDRYDPIVGSSSEVGFVGATPEGVPVVAIFEWGAKYKRAWIYRSTDGMLL
                     YWMDEKLVDPSTGMVQQAEWQLSGE"
     gene            complement(7673..8413)
                     /locus_tag="PLM74_00950"
     CDS             complement(7673..8413)
                     /locus_tag="PLM74_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HON41684.1"
                     /translation="MHNIGVPALDPDPDWRHRTALLIGEQGLDRLAQARVIVVGLGGV
                     GSYCAEALARAGVGSLVLVDGDSVSITNINRQLLALVDTVGLPKAEVMADRIRRINPQ
                     CDVAPMVMWVDGGNVDSLLGSGPNSAQFVADAIDTIPAKLDLIETCLRSGVGAVSSMG
                     AGNRLDPTRFRVADISQTHTCPMARAVRQGLRRRGIPSGLTVVFSDEPPVVQRPGPVG
                     SISMVPGAAGLAMAGVIIRHLAFASSES"
     gene            8541..9362
                     /locus_tag="PLM74_00955"
     CDS             8541..9362
                     /locus_tag="PLM74_00955"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HON41685.1"
                     /translation="MANLVAPGSVDVVVTSPPYNLGIEYRSYNDRISRNEYLAWTIEW
                     ASCVARVLAPTGSFFLNVGSKPKDPWVPFEVASALRGIFELQNTIHWIKSIAISRADV
                     GDYPGLEGDIAVGHYKPINSGRFLNDCHEYIFHFTLTGNVPLDRKAIGVPYQDKTNIA
                     RWASTRSNLSDLRCRGNTWFIPYQTIQRATDERPHPATFPPRLPEMCIRLHGLDRCGL
                     VMDPFSGLGNTGVACALLGVPFVGFEIDPVYFAESVKRIGRAAGQGSGAVTRELE"
     gene            complement(9398..10345)
                     /locus_tag="PLM74_00960"
     CDS             complement(9398..10345)
                     /locus_tag="PLM74_00960"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HON41686.1"
                     /translation="MFIMDAHCDTLLDIAAGRRRLSERGKGGHIDLPRMVDGGVSSQI
                     FAIYVEPEYLPGAGARQALRLVDVFCREIEENGDSIVFAKWAGDAGRARDEGKVAAFL
                     SIEGGEALDGSIELLRIFYRLGVRLMTLTWSRRNELGDGAYEEGTRGGLTRFGVDVVR
                     EMDRLGMVVDVSHLSEATFWHVMDVSTRPLIASHSNARALCGHSRNLSDEQAKAIADR
                     GGVIGVTFVGPFLGEGRQSVQGVVDHIDHFAGVIGTDHIAIGSDFDGVYDSALPEGLH
                     DVSRMPALIDELARRGYSEAAVRGIMGENLLRVVEGVIG"
     gene            10496..11353
                     /locus_tag="PLM74_00965"
     CDS             10496..11353
                     /locus_tag="PLM74_00965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HON41687.1"
                     /translation="MSGISIMTDSTADLSRELIARYGVTVVPLAVVFGQDAYKDGVDI
                     DTQRLFELVEQNKQLPKTSSPSPEVFRSVFEEAVAHGHQVLYIGISSKFSASVQNAAL
                     VAGAFPEGQVRVFDSANLSTGIAHLVLYARDLVSQGRQMDEILAALERARPKVRTSFK
                     LDRLDYMHMGGRCTGVQTLLSSVLKIRPVISVADGGMIVAGKVRGSSRKSLDWMMDRF
                     EEDVRQGLVREDRVFLTHTGVHEDAVYMAERVREIMPEMKEILETPAGSVIGSHCGPG
                     TIGILYILD"
     gene            11474..11698
                     /locus_tag="PLM74_00970"
     CDS             11474..11698
                     /locus_tag="PLM74_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 family protein"
                     /protein_id="HON41688.1"
                     /translation="MSQRIGSALFQIGGMMFLIGLILVRFPGAFSWFGRLPGDIITEH
                     VIAPFTSMLIVSAGLSGLSWVFSVLLRLMR"
     gene            complement(11951..14296)
                     /locus_tag="PLM74_00975"
     CDS             complement(11951..14296)
                     /locus_tag="PLM74_00975"
                     /inference="COORDINATES: protein motif:HMM:TIGR02887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009847 - spore germination [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ger(x)C family spore germination protein"
                     /protein_id="HON41689.1"
                     /translation="MRGAGGAVSARSIGALVFLARLLPIVISLPTMTPNVPHQSVWIA
                     GVIGVALAVPMMWAMAAIGERDPKKSLLAQSRDLLGAWPCAAIGIVLCAYWVITAATF
                     LKAMSQAFLATALHSTPSLAIVAPLAVLSATIARRGIGLICALADLIAPVVMASVALI
                     IGLPHQAAKLINLRPLLPQGLSALTAPALTTTSFFALASMAWMIIPRCAEARQGGVRR
                     WFLIAVAGAGVLAVGLMAVVGAVFGPLSSTIGFPLYSVTRFISIGRVLERLEILPMGF
                     WVFAAGTVVASFYWAAAQALGEVVQVERSGMFVYPLAAVSIALGETMFRRVMVLSRFM
                     SLEGWGAFSLALVIAIMAAMAIAWWARRRRAARALVSIAAIAILSILASGCWDRREIE
                     TLGFVVGCALDTADAGDQPEDAGFVQVTVQIAKPWAMGMGERAGSPERAFYTVTAVGS
                     TVFEAVRSINEQSTRTPYWPHSRWIIIGEDLARAGIESVLDFFVRNRGPRELPVLGVL
                     DGGRGWDVMWAEFELERMPAIGGEGLISGVLRDLGTTVRTTLGDFLVSLADPGVDPVL
                     PRGVIVAYSDEPAATGDLLREKIRTKPEMSGAGVFRGDRLAGWLTGVETRGLNWVRGD
                     VVSAVIAVPAPGAEDPGDFRNRVSLEIMRASSQVSVRALNTTDEVEARVKVDVTAHLA
                     ETRSFVDISQDMQALIQMEEALADQIRGEILGAVQKAQSLGADIFGFGRMLMASDYRA
                     WKRIHPKWDSVFAGLPVKVEVKARIDRSALETNRYEADTTK"
     gene            complement(14293..15819)
                     /locus_tag="PLM74_00980"
     CDS             complement(14293..15819)
                     /locus_tag="PLM74_00980"
                     /inference="COORDINATES: protein motif:HMM:NF015292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HON41690.1"
                     /translation="MRNVRKTRESNRQAEPSLPDDVQQRAEAVRSLFGASADVVVRTY
                     VAPNAERPVAATFHVDGMVDADLLARCVTGPLAVAVQAYSGADCGAPFNMPASAFHSP
                     DVRRISNVGEAAGMAIRGHTIVILEGDSAMLVSNTAAVIGRVIEEPTTESTVRGSKEG
                     FVENLRTNTALIRRSIADPRLRVEEMSIGRTAHTRVATLHVDGIVNSMVLTEVRERLA
                     MIDVDALHESGHLEELIEDAPYSIFPTMMRTERPDRAAGALLEGRVVLVVDGTPFVLI
                     VPVTFTMFLTTSEDYFERFPLGTLARLLRVVAFALSMLLPALYVAVTTFHQEALPTPL
                     MLSIAAQREGIPFPAVFEALIMELTFEVVREAGIRLPRLVGPAVSIVGVLVLGDAAIR
                     SGLVSPIMVIVVAGTGVASFATPSFSMAIGARLLRFAFLFLSAALGLYGIAIGVFAFL
                     AHLTAMESFGVPFMAPLAPVVPSDLKDSVVRAPWWAIRRRPESVRPVDSRRARGEERR
                     "
     gene            16066..>17489
                     /gene="nagA"
                     /locus_tag="PLM74_00985"
     CDS             16066..>17489
                     /gene="nagA"
                     /locus_tag="PLM74_00985"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HON41691.1"
                     /translation="MNSFLIAGGAVVSASGLSTDTSVLIQGAKIVEVGPSCDLAGRAP
                     GTTIDARGAIVAPGFIDMHIHGSAGFDTMDATPSALARMAEFISSHGVTGFLPTVMST
                     PIEQMLAATHAAAQAAQADINMHGEHSEHDRPRKGAQVLGVNVEGPFLSPDFKGAQPE
                     TGIVGPDPAVLHRILEAGGGHVRVMTIAPEMPGAIEIVKALASQGVLASVGHSGACYD
                     EVRDAIHAGLRLVTHTYNGMRGLHHREPGVVGAALVHSELTCEVIADGIHVHPAAAKL
                     VAQAKGPGGTVLMTDAMRAAWQTWRDDPDGPYAKASPEARRRADRVLMADTARAFSLA
                     AQKDVALSEARRRTAAEASLATAERAASMYARDDALFTSAAADAADQAARLKLGRLIA
                     NPGEADPEKLVFKTEDGAALYAAVRADALDRLRHGRARTLARDAGVAGNEADAASGIA
                     AAARVAETLPPDLRADALAELETVR"
BASE COUNT         3591 a         5329 c         5134 g         3435 t
ORIGIN      
        1 cgagcccgtc gagcgaatcg tgacgcacac tgctggagct caccgttgcc aggcaagtgt
       61 gaccgtaaac ggaccacatc ctgcggtcat caatgacgcc gcactgtccg cgctggcagc
      121 caggtcagtg cagactgcac tcggccccgg gtatctggcc gcttgcgagc cctggatggc
      181 ctccgagacc atggggtgct atctctcaaa atacccaggc gtcttggcgt tgctggggat
      241 agcctgtgat aaggtgggcg ccggagcgcc ccaccacagc cccagtttcg atattgatga
      301 atcggcgctg aagtatggcg tggcggctac gctccagttc gcccgggatg tcctgctgca
      361 atcgccctag tgcatctgtg cgggagcaac cgcgttctcc ggggaggccc ccttgatcaa
      421 cctggacaga ggcttgtata tggccgcagt gatcgctgcg ttgatccccg ccttcaccgc
      481 gttgaacgcg attatccatc ccagaaggct gaccacagcg ctggtgggaa tatgatagaa
      541 ccgcggtgtc aagataaggt ttgcagggat catcactccg accatggcgg ctgtgcctgc
      601 tagcaaggcc cagaaggcgc cggctctgga cctgaacctg ctgtagatga tggacgacac
      661 tccgaccaag gctcccgatg atatgaggtg catcactgcg ccgaaccacc cgccctcagg
      721 gtgcagaggc gcctgcagca cgcagactac cgcggtacag gctagcccgg cccatggccc
      781 gagcgcaaat ccggcgacca ggagtcctac gtctgctgcc tcgtacacga gaaacggaac
      841 cgcggggaag atcggcgtgc gaaaaaccag gaacaacacg atcgaggtag ctgtcaagac
      901 tgccatggtg gacagacgcc taagctctgt tttactatct cgcttcattt gaagacctcc
      961 tgacgctcga cttaatcgaa aagctcccag accgaggcct gggagctaat agtcgaggcg
     1021 ctgcaaggca gcgcctaacc ttctcgtatc cggactgtac cgtcggttct ggcttctcac
     1081 cagatcggcc gcatcgctgc ggttcgtgga cttccccccg cgctgtcatc tgtgcgcggg
     1141 gtttcaccac cggtcgggaa tcgcaccctg ccccgaaggc cacgatatgc gctttttcgt
     1201 gcattatgat atcacgagcg gttcatagga tcaaggctca tccgtgctgt gtgctgctgc
     1261 tcacacccgc tgtgcgctgg ccgttcggcc gcgctgtagc caccgattga caacatcgcc
     1321 cgattgggcg atgcctatgg gccgggcatt actggtataa tgaactagcg agtctgggag
     1381 gtgtagtgat tgtccgatac tggcaagaat cgccgcaggc gtgcagcctg ggtgcttgta
     1441 gccatagcag cgttggcgct gatcatatgg ggcgctatca ggggcaggaa caagtcgctt
     1501 gtagaggctc gactgcccgc cgccacggcc gggcttgcga cgacgtctgt ggaccaggga
     1561 gacgtcaatg agtacgtatc ctgcacagga agcttgatgc ccattcgtat gcaggttatg
     1621 gcatccgatg cgtccggagg agttaccagg gtgtatgtga aagaaggcgc ccgcgtcgcc
     1681 cgcggagcgg cgctgtgcga agtgcagcgc tcttcggctt tcgatttctc atcccagagc
     1741 gacaaaacca ggctgacggc gccgtttgac ggcgtggtct cgtcggtcac ggttcaagca
     1801 ggcggttatg taggcgcagg ccagccggcc gtcaccatca tggatacgtc ttcatacttc
     1861 gcgagtgcag agatcgacga gatcgacctg gcacgagtag agccgggaat ggctgcaatc
     1921 atgtcattcg acgccctccc cgatgtggag ttgcccggca aggtagaatg gataggcgtc
     1981 gccgccctac cccgtgccgg gatggttgtc atccccatca gagtgtccat agatgggtcg
     2041 catgacctgc tgaaaccagg gcttacgacg aacgtaagga ttctttcccg cagcatccag
     2101 ggtgtggtca ggatttccgt ccgctcgtgg gtcgacatgg atggccagcc atcggcaatc
     2161 aggatcgtgg gcggagcgcc aaagctgatc cccgtcgaat tggggctgtc tgacggcaac
     2221 tacactcatg tgctgtccgg gctttcgccg ggtgatgaaa tcgtggagga tgctgtagca
     2281 gcccaggaga agctgcgtcg gctgaccggg caggaccgcg atatgcaatt cagaatcagg
     2341 catgccgacc agtagtgcgg aggagcatca cagtgatcaa tctcgagagc atcgcgaaag
     2401 tgtactcaat gggctcagtc gaagtgcacg cgctcgatgg ggtgtcgttt gacatctcag
     2461 atggcgatta cgtgtcgata atggggccgt cgggctccgg caaatcgaca ctgatgcata
     2521 tagtcggctg cctcgaccgt ccgagttcag gggtataccg ccttcatgga gacgacgtgt
     2581 cgcagcttga cgatactgaa ctggcgcgca tacgcaacag acgaataggt ttcgtatttc
     2641 agcagttcaa cttgctgcct cgcgagacag tgctcggcaa tgtggagata cctctgatgt
     2701 atgcaggagt gggcggcagg gataggcgac gaatcgccgg ggaggccctc gcccgggtgg
     2761 gcctggccga ccggatgcac caccggcctg ccgaaatatc gggaggacag aaacagcggg
     2821 tcgccattgc aagggcccta gtcaacgatc cttcgctgat cctcgcggac gagcccaccg
     2881 gcaatctcga ctcaaagacc ggtggtgaga tactggcgat cctcgatggt ctgaacgccg
     2941 agggatgtac catagtgtta gtcacacacg accgatcggt ggccgagcgc gcgcgtcgga
     3001 tagtacagtt gcgggacggc tcggttgagc gcatagagga gctgggcata gagcaaccgg
     3061 ccgaaagggg gtcacggtgc gatgggcata gctgaaacca tcggggtggc cctgaggaac
     3121 ctctgggcca acaagctccg cacagcgctc tcagtgctgg ggatcgtaat aggcgtagcg
     3181 tcagtgataa ccatgatctc ggcgggttcc agcgcccaga agcagatcac gtcagacata
     3241 tccgcgcttg gctccaacat gatcatggtg actccgaatc tgaaaccagg catgcaaggg
     3301 cgaagtcacc tacatctgct gaccatgtcg ctggcagatc agatacaagc cgcggcgccc
     3361 gcgatatccg ccgtctctcc cgtagcgcag atggaggggc gcatcatcgc aggcagcgcc
     3421 agcgcttcgg ctgtgataac tggcatcact cctgcgtaca caaggatcgt cagcacccga
     3481 atcgcgttgg gccgcagcct tattcagtcg gaagtaaacg cagctgccca ctctattgtg
     3541 ctcggctcag aagtagcgtc cgatctcttc ggcccggacg acccgatagg gcgacaagtg
     3601 gcgttggtgt cgggcgatcg ccgagcagtt ttcaccgttg tgggcgtgac tgagtccaaa
     3661 gggggcactg ttttgggcta atgccgatac gcaggctttc atcccggtga ccaccctgct
     3721 gtacaggatg tcgcctatca agcgtgtgag cacctatttc tgccaggccg catctgcgca
     3781 ggcgacggat gtagcggtag cgcaggttga gcggttcctg gcttcggttg caggaacacc
     3841 cgatgccttc agcgtagcgt ctcaagcagg tctgatcgac gcgataaagc aggtaacagc
     3901 caccatgacc ttgcttctgg gcactatcgc cggcatcagc ctggtagtag gcggaatagg
     3961 gatcatgaac accctgctta cctcggtcgc ggaacgtacc cgcgagatcg gggtcaggaa
     4021 atccctagga gcctgcaatc gcgacatcct gctacagttt ctgtcggagg ccctggttct
     4081 gagcatgctg ggcgggactg tgggtatgct cctgggatgg ctgggtggag ctggaatagc
     4141 gcgaatcgca ggttggccag tatcggtgga tctgagttcg gtcatagctg ccgtgtcgtt
     4201 ttccacggca gtggggctca tattcggcac gtaccctgcg ctgcaggcat cgcggatgga
     4261 ccctgtgcag gcgctgcgat acgagtagtc agctcaaaga acggaggcgt ttctgcatgg
     4321 catcgacgat tcgcggtaga gtgggtggag tgatcggtgg gtacagcgtg tcgtgcataa
     4381 ccacgcctac cagcgtttcg ggccgagtag gcggcgccgt tatcgggggc gacctgaacc
     4441 ttgatattga accacctccc gggcgcatat tcgggcgggt gggcggcgcg atcatcggga
     4501 gaaccgtaga tgcgcggttc gcggattctt caggtaacag cgtccatggt cgcatgggcg
     4561 gtatcattga cggcgtggac ataacgctga gcggaaccga ccgcatcacc ggaagatgcg
     4621 gcggtcccgt cattggtttc gatgtagacc ttgaagtggc ggacggtcag atgaccgggc
     4681 gcctcggagg agcggtagtg ggccactcag tgtcgttcag tatggccgca ggccttccca
     4741 acgtggtgac ggcgcttgtg gccgtcatag cataccaata ctacctcata gcgcagcgca
     4801 agagctcatc atcatcatca tcatcgtccg gaagtggcgg caattagcga tcctggcggc
     4861 ggatcagctt ctcgtacgca gaattcgcac tgcaacgggc ccaaaaaggg acagggccgg
     4921 atcgcgcctc gtcgtccggc cactgcccta gtgcccaatc aagttctgtc gccctgctag
     4981 taggtgaaca gataccacca ttcggatccg ctttcgcctc tgtagatctc tggattctgc
     5041 gtgccgggtg aataatactt ggtccaatgg gaggcgtagt tgaacgcttc ctccgtatcg
     5101 attgcgtggt tcggtctcgg aagatcagcg tcggccgggt aagggtatct catgccctcg
     5161 gtgagagccc acgtgaacac cccatgaccg ccaatgggag gtctgcttga ctcccacgaa
     5221 ttctcatctt tggcgcatgc ggtcattatg atcttgcgcg gtccggtgat attcctggcc
     5281 ataacctcga agcctgtgga ccatgtgaac cccttcggta gagccgcgag gctcctgtac
     5341 atgccgcctg aattgcatga atcgaagatg aatattttct tggcgtatgc gggcatgtac
     5401 tggttgatcc agttgttcag gtcctggtcg gatatcatcg tgcccaggtt aagcgggttt
     5461 gcatcgctcg gtacgatgta ttcagtagtc cctgcgtcgt tgtgcgttcc gtgccctgag
     5521 tagaacatga tgaacgtgtc gccattcgtg atattgtagc cgatgtctcg gatcgcattc
     5581 tgtatcgagc tcttcgtggt atcccactcc gcagtcagta gcgtgtactg cttcgcgagc
     5641 ctgttctcca cgcccaggcg cgtaacgatc agctcggcgt cagcaacggc ataagatagg
     5701 tcggagatat agccgctctg gtagtcgttg ataccgatgg ccaatatgtg gagggaccca
     5761 agcatcggcg ttccgcctat gcgcgtggtg cccccggctt cagcatagca ggacttctcg
     5821 tatacatcga gatacttctc gtgtgtaatg gtaactttgt agtagcctga ggagagcctg
     5881 ccgattctga aatacccatt ggaatccgac ctcgcgattg cgctcccgtt aggcccctcc
     5941 gcgacgacat atgccccggc gagaggggcc aggtttccct tcggagcctc ggccatcacg
     6001 ccgaatccgg gctcgccctc ggcctgagca gatctggcgt ctgcatgctt ccatacgtag
     6061 ccgtctatcg cacccgtaat aagaccgggc ccgccacacc cggttgtcgt ggccaccaca
     6121 acaagcaaca gcagcagcaa cgcggcgatt ctccgaacct tcatcgtgca acctcctcga
     6181 cacatgtcac ggcaacaata tccgccaaac cgtccagtct gcacctgagc acaccccggc
     6241 ctgtacttga ttgtgactcc ggacgtggcc caatgttccc ccaaacctca ctggacggta
     6301 tatcgcgtat actcctgaaa cagcaggaaa acgctgaacc tctgaggaat agtcacatga
     6361 gtatgcatca tgagcagcct ctcactgggg ggatatcgtt gtgcttcgaa ctcgactagc
     6421 accagcttgc tgcatagctc tattgctatg tctgatcaac atcgcgcccg gcatggcgat
     6481 cgcgcctggc acgaccggtt tccattcacc cgtcggctcc cgcgggccga gcctgctgga
     6541 ccgctcagct gaagccggtt cagccgtaga tctcatgccg gaagctaaga gccttcaggc
     6601 gccgtcatgg gttcgcgaag ggttgagagt gtactatcgt acttcctcgg ccactataga
     6661 gaacccgttt gctgccgcaa gcaacgcccc caaacccccc gtagtcaggg tggaccaggt
     6721 gagtacgtct gctccggccg ttgtgagaac cgacgttatc gcgctggcgc cgcggttttc
     6781 tgcgacgtac tccacgatct tcatcggcga tctgatgatc ccgtgggatg acagctcgtc
     6841 cagcgggttt gccgggatag gtgagttctg gatcaacccg gctgcgctgc gcgacgcggc
     6901 gaaacggtcc tcttccggac tgtcggcaga acctgtccac ttcgaagttg gagggaaggc
     6961 ctattccgcc ctgcgtctcg attccacaca ggcgaacccc acgcgctacc tcacctgggt
     7021 tttcgacacg tccacaggtc tgcttctgta tcaagctcaa tggatggcac tagcggatcg
     7081 cggtcttgcg cacctgtcag ttgccgaatt cgtgtcgatg cgcaacatga cgctcccctg
     7141 ggatggcgga gtgacgccac catgggtgaa acagggtacc aggttaacgt accaaggaag
     7201 catccagaca tggactccgg caggcggacg caatatcccg atcccggctg tgctcgaggt
     7261 ggagatgacc tcggtcgggc gcaactggtc tgcggccagg gtaaagcggg tgttcagcag
     7321 cgacggcgag gatcaggcta ttaccgtcta ttccggcacc gggctactgg ccggcggttt
     7381 ctgggttccc cgcgccaacc tcgccaggct gcgcagcggc gagcggttgg accgatatga
     7441 tccgatagtc ggcagcagca gcgaggtggg ctttgtggga gcaacccctg agggggtccc
     7501 tgtcgtcgcc atattcgagt ggggcgccaa gtacaagcgc gcctggatat accggtccac
     7561 ggatggaatg ttgctctact ggatggacga gaaactggtt gacccgtcca cgggcatggt
     7621 ccagcaggcg gagtggcagc tgagcgggga ataggcctaa accggccaga gcctagctct
     7681 ctgacgatgc gaaggcaaga tggcggataa tcacgcctgc cattgccagg ccggctgctc
     7741 cgggaaccat ggatatgcta cccaccgggc cgggtcgctg gaccacgggg ggttcgtctg
     7801 aaaataccac tgtcaatccg ctcggtattc cacgcctgcg tagtccctgt cttaccgcgc
     7861 gggccatggg gcaggtgtga gtctgcgaga tatcggcaac cctgaacctg gtggggtcga
     7921 gcctattgcc tgctcccatt gacgataccg cgccaactcc tgaccttaag catgtttcta
     7981 tcagatcgag cttggcgggg atcgtgtcga tggcatccgc cacgaactgt gcggagttgg
     8041 gccccgatcc gagcagacta tccacgttgc cgccgtcgac ccacattacc attggggcaa
     8101 cgtcgcactg ggggttgatg cgtctgatcc ggtcggccat gacctcggcc ttgggcaacc
     8161 cgactgtgtc cacaagcgca agcagttgcc tgtttatgtt ggtgatcgat actgaatcac
     8221 catcgaccag cacaagtgag cccacgcccg cccgggccag cgcctcggca cagtacgatc
     8281 ctacgcctcc caggccaacc acgatcaccc gggcctgcgc gaggcggtca agcccttgct
     8341 cgccgatgag caacgcagtc cggtgacgcc agtcgggatc ggggtccaag gcaggaacac
     8401 cgatgttgtg catgcccgga ttctccttcc acagcaagag aggtgccgtt gatgtgactc
     8461 aacacaagaa tccaaccaca atcgaactcg cttcaggctc gggcgcgctg accctgcata
     8521 actgcgattg catccaaggt atggccaatc tagtagcgcc ggggtcggtc gacgtggtag
     8581 ttacgtcgcc tccttacaat ctgggcatcg agtaccgaag ctacaatgac cggatatccc
     8641 gtaacgaata cctggcatgg acgatcgagt gggcttcatg cgtggcccgc gtactcgcgc
     8701 cgaccgggtc gttcttcctt aatgtcggct ccaagccaaa ggatccatgg gttccgttcg
     8761 aggtcgcaag tgcattgaga ggcattttcg agctccagaa caccatacat tggattaagt
     8821 cgattgcgat ctctagggcg gacgtgggag actacccggg gctcgaggga gacatagcgg
     8881 tcggccacta caagcccatc aacagcggca gattcctcaa cgactgccat gagtacatct
     8941 tccacttcac cttgaccgga aacgtaccgc tggacagaaa ggccatcgga gtgccctatc
     9001 aagacaagac caacatcgcc cgatgggcct ccacacgcag taacctctct gacctgcgtt
     9061 gccgcggcaa cacatggttc attccttacc agaccattca acgtgccaca gatgagcgcc
     9121 ctcacccggc caccttcccg ccgaggctgc ccgagatgtg catcaggctc catgggctgg
     9181 ataggtgcgg cctcgtgatg gaccccttct caggcctcgg aaacacagga gtggcctgtg
     9241 ccctgctggg agtcccgttc gtcgggttcg aaatcgaccc tgtgtacttc gcagagagcg
     9301 tcaagcgaat cggccgcgca gccggacagg gcagcggggc ggtcacgcgc gagctcgagt
     9361 agtccggtcg cgccagggcc gatagagcat gcctagacta ccctattacg ccctcgacca
     9421 cgcgcagaag attctcgccc atgattcctc gcacagccgc ctccgaatag ccccgtcggg
     9481 ctagctcgtc tatgagcgca ggcatgcgcg aaacatcatg taacccttcg ggcagagcgc
     9541 tatcgtatac tccgtcgaag tccgatccaa tggcgatatg atctgttccg atgacgccag
     9601 cgaagtgatc gatgtggtcc accacgccct gcaccgactg cctgccctct cccaggaacg
     9661 ggccgacgaa ggtgacccct atcacaccgc ctctgtcggc tattgccttg gcctgctcgt
     9721 cgctgaggtt gcgcgaatgc ccgcataggg ccctggcatt ggagtgcgac gctatcaggg
     9781 gccttgtcga cacgtccatg acgtgccaga aagtcgcctc cgatagatgc gaaacatcca
     9841 ctaccatgcc aagcctgtcc atctcgcgga ctacgtctac tccgaaccgg gtgagcccac
     9901 cgcgcgtgcc ctcctcgtat gccccgtctc caagctcatt tctgcggctc cacgtgagcg
     9961 tcataagcct cacgcccagc ctgtagaaga tcctcagaag ctcgatcgat ccgtcgaggg
    10021 cctcgcctcc ctcgatgctg agaaacgcgg cgaccttgcc ctcgtcgcgc gcacggcccg
    10081 catcgcccgc ccacttggca aacacaatcg aatcgccgtt ttcctcgatt tcgcggcaga
    10141 atacgtccac aagtcgcaga gcctgcctgg ccccggcgcc cggcagatac tccggttcaa
    10201 cgtagatcgc gaagatctga gaagacaccc cgccatccac cattcgcggc agatcgatgt
    10261 gtcctccctt gccgcgctcc gacaggcggc gacggcccgc ggcaatgtct agaagcgtgt
    10321 cgcaatgagc atccattatg aacatgactg gctactacac ccccgatcac tccatgtgcg
    10381 ccatgtgtcc agtataccac cactcacgcc agtgttggcc tcgtttatgc aggcatggta
    10441 aaattggtac agtcgattac agctacacca tgtcgacaac tggagggatg acgatatgtc
    10501 aggcatatca atcatgaccg actccactgc cgacctcagc cgggaactca tagcccgcta
    10561 cggggttacg gttgtgccgc tggcggtggt gtttggccaa gacgcgtaca aggacggcgt
    10621 agatatcgac acacagcgcc tttttgagct ggttgaacag aacaagcagt tgcccaagac
    10681 ctcaagcccc agcccggaag tgtttcggtc tgtattcgaa gaagccgtcg cacatggcca
    10741 ccaggtactc tacataggga tctccagcaa gttctcggca tccgtgcaga acgcggcgtt
    10801 ggtggccggc gctttccccg aaggacaggt gcgcgtgttc gattcggcga acctttcaac
    10861 gggaattgcc catctagttc tgtatgcgcg cgacttggtc tctcaaggcc ggcagatgga
    10921 cgaaatactt gcagcgcttg agagggcgcg tcccaaagta cggacatcat tcaaactcga
    10981 tcggcttgac tacatgcaca tgggcggaag gtgcaccggg gttcagacgc tgctgtcgtc
    11041 ggtgctgaaa atcaggcccg tcatatccgt ggcagacgga ggcatgatag tcgcaggaaa
    11101 agtcaggggt tcaagcagga aatcgctcga ttggatgatg gaccgtttcg aagaggatgt
    11161 ccggcaaggt ctggtgagag aagatagggt ctttcttaca cacactggag tacacgaaga
    11221 cgcagtgtac atggccgaac gcgtgcgcga aatcatgccc gagatgaagg agatactcga
    11281 aacgcctgcc ggctcagtga taggtagtca ctgcgggccg ggaacaatag gcattctcta
    11341 catactcgat tagccattat attacttcat tctggatcaa tcggcagact cagcgcggcc
    11401 cgggcccggc ctaccgtgtg cataaagccg cgggggcacc acccgggtcg cgcagttatc
    11461 gggggtggaa accatgtcac aacggattgg ctcagccctg tttcagatcg gcggcatgat
    11521 gttcctgatc gggctcatac tcgtgcgctt ccccggcgcg ttttcctggt tcgggcgcct
    11581 ccccggcgac atcataacag aacacgtgat cgctcccttt acctcgatgc tcatagtcag
    11641 cgcaggcctt tccggcctgt cgtgggtatt cagcgtactg cttcggctca tgcgctgacc
    11701 aaagacgaaa tgcagcaaca gcttcatagt gaaaatagcc gaactagcgc ttttccgatc
    11761 actgatatcg ggactattgg gttagggaca ggccagccca tggctgcagc gaaacagatc
    11821 gcgaaggcca gggccattac cgacacaaaa gctaccaaat cacggcgtga tccaccgctt
    11881 gtcagcggct tgtattcaat gaaccagatg gctgcaaaac cgaccaacac caagagaacg
    11941 gaaggtatac tcatttcgtc gtatctgcct catacctgtt tgtctcgagt gcgcttctgt
    12001 cgattctggc tttcacttct accttgacgg gaaggccggc aaatacacta tcccatttgg
    12061 ggtgaatcct cttccatgcc cggtaatcgg aagccatgag cattctgccg aagccgaata
    12121 tgtcggcacc gagcgactgg gctttctgca ctgcgccaag gatctcgcct cgaatctgat
    12181 cggccagcgc ctcttccatt tggatcaggg cttgcatatc ctggctgatg tcaacaaatg
    12241 atctggtctc ggcaaggtga gcggtcacat ccactttcac cctcgcttcg acctcatccg
    12301 tcgtgttcag cgccctcacg ctcacctgcg agctcgccct cattatctca agagatacgc
    12361 ggttccggaa gtcaccagga tcctcagccc cgggcgccgg cactgcgatt accgcgctta
    12421 ctacatcgcc tcgcacccag ttgagccccc tagtctcgac tccggtaagc cagcccgcta
    12481 ggcgatcgcc tcggaagacg cccgccccgc tcatctcggg cttcgttctg attttctctc
    12541 tcagtaggtc gcctgtagca gccggctcat cagagtacgc tacaatgact cctcggggca
    12601 gcaccgggtc gacacccgga tcagccaggc tgacaagaaa gtcgcccagc gtggtccgaa
    12661 cagtggttcc caggtctcgc aaaaccccgc tgatcaatcc ctctcctcca attgctggca
    12721 tgcgttcgag ctcgaactcg gcccacatca catcccaccc gcgccctcca tcgagcacgc
    12781 caagaaccgg gagttccctc ggtcctctgt tgcgcacgaa gaagtccaac acgctttcga
    12841 ttccggcgcg cgccaagtct tcgccaatta tgatccatct gctatgcggc caatacggcg
    12901 ttctggtgct ctgctcattg atgcttctaa ccgcctcgaa tactgtgctt ccaacagctg
    12961 taacggtgta aaatgctcgt tcggggctac cggccctctc ccccattccc attgcccacg
    13021 gtttcgctat ctggaccgta acctgaacga agccggcgtc ctcaggctga tcgccggcat
    13081 cagctgtgtc caaggcgcac ccgacaacga atcccaatgt ctctatctcg cgtcggtccc
    13141 agcaacccga ggccagtatc gataagatgg ctatggccgc tatcgatacc agggctctgg
    13201 ctgcacgccg gcgccgcgcc caccatgcga tcgccatggc agccatgatg gcgatgacca
    13261 gagccagaga gaaggcgccc catccctcaa gcgacatgaa cctgctgagt accatcaccc
    13321 ttcggaacat ggtttctccc aaggcgatgc tgacggccgc cagagggtag acgaacatgc
    13381 cgctcctctc gacctggacg acttctccga gcgcctgcgc agcggcccag tagaagcttg
    13441 ccaccacagt ccctgcagca aatacccaaa accccatagg caatatctca agccgctcta
    13501 ggactctgcc tatgctgatg aatctcgtaa cggagtaaag tggaaagcca atggtggatg
    13561 aaagcggccc gaacacggcg cccaccactg ccataagccc gacagccaaa accccggcgc
    13621 ctgcaactgc aatcaggaac cacctgcgaa cacccccctg cctcgcttcg gcacagcgcg
    13681 ggatgatcat ccatgccatg ctggccagtg caaagaagga agttgtcgtc agcgccggcg
    13741 cggtgagcgc cgagaggcct tgaggaagta gaggcctgag gttaataagc ttcgccgctt
    13801 gatgcggaag accgatgatg agcgccactg aagccatcac cactggggca atgagatccg
    13861 caagcgcgca tatcaggcca atgcccctgc gggctatggt cgcgctcaac acagccagag
    13921 gcgccactat ggcaagcgat ggggtggagt gcaacgcggt cgccagaaag gcttgggaca
    13981 tggccttgag aaacgtggct gcagtgatga cccagtatgc gcagagcacg attccaatcg
    14041 cagcacacgg ccaagcgcct agcaagtcgc gtgattgggc cagcaggctc tttttcgggt
    14101 cgcgctcgcc aatcgccgcc atcgcccaca tcattggcac tgcaagcgca acgccgatta
    14161 ctcccgcgat ccagacgctt tggtgaggga cgtttggagt catcgtcgga agagatatga
    14221 cgataggcaa cagcctggca aggaagacga gcgctccaat gcttcttgcc gacacagccc
    14281 cgccggcgcc tctcatcgtc tttcctctcc tctcgctctt cgtgaatcta cggggcgaac
    14341 cgactccggc ctgcgcctga tcgcccacca tggggcgcgt accacactgt ccttcagatc
    14401 actgggcact actggggcta gaggcgccat gaatggcact ccgaacgact ccatcgccgt
    14461 taagtgggct agaaacgcga aaactccaat ggctatcccg tacaacccaa gggccgctga
    14521 taagaacagg aacgcaaatc tcagcaacct tgcgcctatg gccatgctga atgagggagt
    14581 ggcaaaagat gcaactccgg tgccggctac aacgattacc ataataggcg agaccagccc
    14641 tgacctaatg gcggcatcac ccaacacgag tacccctacg atactgacag ctgggcccac
    14701 cagccttggg agacggatgc ccgcttctcg cactacctcg aacgtgagtt ccattatcag
    14761 ggcttcaaac actgcaggaa agggtatccc ctccctctgc gcggcgatac taagcatcaa
    14821 cggagtagga agagcctctt ggtgaaacgt agtcacggct acgtacagcg cgggcaacag
    14881 catcgataat gcaaaagcca cgactctcaa tagcctcgcc agagtgccga ggggaaagcg
    14941 ttcgaagtaa tcctccgacg tcgtcaagaa catggtgaat gttaccggaa caatcaggac
    15001 aaacggtgtt ccatctacaa ccaagaccac cctgccctca agcaaagcgc ctgccgcgcg
    15061 atccggacgt tctgtcctca tcattgtcgg gaagatggag tatggggcgt cctcaatcaa
    15121 ctcctcaagg tgtcccgact catgcaaggc gtccacatct atcatggcca gccgttcgcg
    15181 gacctctgtg agcaccatgg agttaacgat gccatccaca tgaagagtcg caacccttgt
    15241 atgagccgtg cgtccgatgc tcatctcctc gacgcggagg cgaggatcag cgatggatcg
    15301 gcgaatcaat gccgtgttgg tgcgcaagtt ctcgacgaaa ccttccttag atccccggac
    15361 cgtcgactca gttgtcggct cttcaatgac gcgtcctatg actgctgccg tgttgcttac
    15421 gagcatggcg ctgtcgcctt caagaatcac gattgtgtgc ccgcgaatag ccatgcccgc
    15481 cgcctcgccc acattcgata tgcgacgaac atcgggagag tggaaggcag atgcgggcat
    15541 attgaagggc gccccacagt cggcgccaga gtaggcctgc accgcaacag ccagggggcc
    15601 ggttacgcac cgcgcaagca gatccgcatc taccatgcca tccacgtgga atgtggcggc
    15661 cacaggccgc tcggcattcg gagcaacgta cgtccgcacc accacgtcgg cacttgcacc
    15721 gaacaggctc ctgacagcct cggcacgctg ctgcacatca tctggcagag agggttccgc
    15781 ttgtcgattc gattcgcggg ttttcctgac gtttcgcatc tgtacgggac gcctgtacct
    15841 cacccacacc accaccgtta ggctgtgctc tgcgtacagt ctgatgtagc catgcaccca
    15901 atatgcaccg ttccccgacg tctgagtgcg caaagagggt ttaccgaaat gtgcggggaa
    15961 ttctactttc ggctgctatg gggatactcc ggtcgcggcg aaacctcggc gacacgccgc
    16021 gggcgccggc agacgagcag cgcctgatag tctggaggga gttgtttgaa ctcatttctc
    16081 atagccggag gcgcagtcgt gtctgcgtcc ggcttgtcaa ccgatacgag tgtactgata
    16141 caaggggcca agatagttga agtagggccc agttgcgacc tggcgggccg agcgcccggt
    16201 acgacaatcg atgcgcgcgg cgctatcgtg gcgccggggt tcattgacat gcacatacat
    16261 ggctcggcgg gatttgatac tatggacgcg accccgtcgg ccttggcgcg catggccgag
    16321 ttcatctcat cacacggagt gactggcttt cttcctacgg tgatgtcgac gcctatcgag
    16381 cagatgctgg ctgcgacgca cgccgcggca caggctgctc aggctgatat caacatgcac
    16441 ggcgaacata gcgagcatga ccgaccgcga aagggggccc aggttctggg agttaatgtc
    16501 gaagggccgt ttctgagccc cgacttcaag ggcgcccagc ctgagacggg aatagtcggc
    16561 cccgaccccg cggtcctcca tcggatactt gaggcaggcg gcgggcatgt ccgcgtcatg
    16621 accatcgcgc ctgagatgcc gggggccata gagatcgtga aggcgctggc gtcccaaggg
    16681 gttctggcat cggtcggtca ctccggggcg tgctatgatg aggtcaggga cgcgatccac
    16741 gcaggactgc gcctcgtgac tcatacctac aacggcatgc gcgggctgca tcacagagaa
    16801 cccggagtcg tcggagcggc tctcgtacac agtgaactca cgtgcgaggt cattgcagat
    16861 ggaattcacg tacaccccgc tgcagccaaa ctcgtggcgc aggcaaaggg cccgggcggc
    16921 acggtgctca tgaccgacgc catgcgcgcc gcctggcaga catggcgcga cgatccggac
    16981 ggcccctacg cgaaggcgtc gcccgaggcg cggcggcgcg ccgaccgcgt cctcatggcc
    17041 gacacggccc gcgcgttttc cctggccgcg cagaaagacg tggcgctctc cgaggcgcgc
    17101 cggcgcaccg ccgccgaggc gtcgctggcc acggcggagc gcgcggcctc gatgtacgcg
    17161 cgcgacgacg ctcttttcac ctccgcggcc gccgacgccg ccgaccaggc cgcgcgcctg
    17221 aagctgggcc ggctcatcgc caacccgggc gaggccgacc cggagaagct cgttttcaag
    17281 acggaagacg gcgcggcgct ctacgccgcc gtccgcgcgg atgcgctgga ccggctgcgc
    17341 cacggtcggg cgcggacgct cgcccgcgac gcgggcgtcg ccgggaacga ggctgacgcc
    17401 gcgtcaggca tcgccgccgc cgcgcgcgtc gcagagacgc tcccgcccga cctgcgcgcc
    17461 gacgcgctgg ccgagctgga gacggtccg
//
LOCUS       DAOTAC010000011        17472 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_2693_length_17472_cov_4.921341, whole genome shotgun sequence.
ACCESSION   DAOTAC010000011 DAOTAC010000000
VERSION     DAOTAC010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 17472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17472
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2693_length_17472_cov_4.921341"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /gene="yedE"
                     /locus_tag="PLM74_00990"
     CDS             complement(<1..485)
                     /gene="yedE"
                     /locus_tag="PLM74_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009428982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YedE family putative selenium transporter"
                     /protein_id="HON41692.1"
                     /translation="MNQRRVVLIAGAVVGALSVALTATGNPANMGICIACFIRDIAGG
                     LGLHRAEVVQYLRPEVPGFIIGSMLAALAAGEFRARGGSSPVLRFVLGFFVIIGALVF
                     LGCPLRMILRLAGGDLNAVFGVLGFTVGIWAGVFFLRRGFTLGRSSRMSIGNAMVLPI
                     V"
     gene            697..1902
                     /locus_tag="PLM74_00995"
     CDS             697..1902
                     /locus_tag="PLM74_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HON41693.1"
                     /translation="MGIDGASKRIYLDNAATSYPKPGEVWESMARYMHDIGASPGRGG
                     YGSSLDAGRLVFEARETAAQLFHAPGAEQVVFTPNVTYALNFAIHGLLRRGDHIVTTS
                     MEHNSVIRPLRSAGESIGAEVTVVKCDAQGRLDPHDVRCALRPNTRMVVMTHASNVCG
                     TVMPIVEVAGVAHEAGVFMVVDAAQTAGVIDIDLRSMAVDVLAFTGHKGLYGPPGTGG
                     MVLSARAATEMASSIQGGTGSRSDEEFQPGFLPDKFEPGTPNTLGIAGLRAGIGFLLE
                     NGVDQIGMHELRLAGEFAKAARAIPGIRVFGLWETGDEAAICARDRVATVSVGFVSAQ
                     ADPARAAFLLDKEFGIMVRSGLHCAPLAHRTLGTFPAGTLRFSFGWFNTMSDVRDAAG
                     ALSCIAERC"
     gene            complement(1899..3143)
                     /locus_tag="PLM74_01000"
     CDS             complement(1899..3143)
                     /locus_tag="PLM74_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41694.1"
                     /translation="MRVSVVEVASSWRDLREVTDRAEHYRAELAVLGASKDIQFQWHD
                     SRVSAMELAGSDALLLLVLSGGTEQLGMSVIARWNGPVAILAHPGDNALAAAMEILAL
                     ARSVGHAGIIVQGSRGWREDIALTVTLAKTYSSLRRAVIASLGSRDIEVMAPWALEGA
                     VREVWGPQLVHLPLGELVRCSREADAEAAQDVAAQMQAAAERVIEPDANAMLGSARIY
                     LGLRRIVQEYHLDAVAVHCFSLLTPLANTACYALARLNDEGVPATCEADIPSCLTMLF
                     MWYLTSQPSFLANPSSIDLRTGTVVFTHCTIPTTMCTRYSLRSHYESNLGVGITGELE
                     PGPVTIVRIGGKELQDLYAVRGQLVECGEREDLCRTQLAVRFDRPEDARILLSRPLGN
                     HHMVTRGDWTHQINSYCDYFHS"
     gene            3365..5353
                     /locus_tag="PLM74_01005"
     CDS             3365..5353
                     /locus_tag="PLM74_01005"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HON41695.1"
                     /translation="MRNAKAIMMLFLLAALVCGIAVSTSGSAADMGQESLPAQTLTQA
                     PALTRVAIDQLLDAFVPSAMARLHVPGVTFVAVKDGEILTMRGFGYSDIEAGRGVDPA
                     ATVFRAASVSKVFTGMAVMQLAERGVVDLDEDVNTYIKGFRIPATYPEPITLRHLLTH
                     TAGLDERNLGMSEFGSRVYPRLGEYLAAELPPRIRPAGQEIQYSNHGAALAGYVVECA
                     AEMPFADYVADKILAPLGMEHSDFRLTPDIEQHLSASYEWRKGKYVNVPYVHINAAPA
                     GALMTTAADMARFMMANLAGGELEGARVLGAEYVEAMQTQQFTNDPRVPGIGYAWFMG
                     RRNGRRVVLHGGDLWQFSTQLLLAPDENFGLFVSGNSSGAAPLADELVKALFDTFFPK
                     HEEPDVSGAVQPAGDVSTLGPAHVAGNPRKLAGVYRTNRRPLTTADKAISLLTQFRVA
                     ANDDGTLTLAFPPGYGMPMATWTPAGPGLFRDRASDDLMAFDHWKTVAGESRPSRMYM
                     GIWAFDRVPVYETASFTLAAVSAIAVVFIWAILAWAFGRRVSGLAAALGLVNLAAIAG
                     IAGSLLAIPGWELTTEVPQMTRAALALPLAGGALALALVWQNIRRIAAERQRQRWIFY
                     NRRARRGLTGLILPWFVIAADGCFLWLLHTWNLLGWRF"
     gene            complement(5383..5754)
                     /locus_tag="PLM74_01010"
     CDS             complement(5383..5754)
                     /locus_tag="PLM74_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41696.1"
                     /translation="MEVREDAVVPLGYGKYVRADRIIALVPIEDDRGPGRRTKVYVEQ
                     LPSPLVASRTENSILASMVRTPRQVLEATAALELIQDLLDDIEQVGPMLRKSIKDEAG
                     LDLDELELKVKEILAHELDSE"
     gene            6039..7148
                     /locus_tag="PLM74_01015"
     CDS             6039..7148
                     /locus_tag="PLM74_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003805585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON41697.1"
                     /translation="MQGMVSVKNLVKTFVSREETVTALNSVTLSTPPNRILTLLGPSG
                     CGKTTLLRCIAGLESPDSGEISIGDQVVWSKAKGISLPPERRGIGMVFQSYAIWPHMT
                     VFGNVAYPLEIRRTPKHEIEERVKKILDLVQLGGFENRLATKLSGGQQQRVALARALV
                     AEPKVILFDEPLSNLDAKLRESTRQELRDFLTQLAISAIYVTHDQVEALTISDDIAVM
                     RGGSIVERGTPQDIYLRSNNPFVVDFIGRANFLSATVKGYGGENHAIVSCALGDIVCS
                     APSGLGVGTEVTIYTRPESFEMVPASSPRMENEFEGTVTSLLFAGEAHEAEISVGGAR
                     VLAKLKSHIPLKKGDKVRLHIDREWCRALNPAEEA"
     gene            7151..8842
                     /locus_tag="PLM74_01020"
     CDS             7151..8842
                     /locus_tag="PLM74_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HON41698.1"
                     /translation="MLNHRSSRRFPLVAFLIIIVSALTIGPVVMLVIGSFSEGIGAIG
                     KFTTGKYVAAYTDPSLPKTLLNTIVFTLGTACFSTALGGLLAYLSVRTDIPFKGLFRV
                     LPIVPMMIPHVLFAAAWIALINPTNGMLNLFLRNLFGFSRGPFNIYSLAGMIFLEGML
                     DLPVTYLVISPAMYSFDTTLEEASRVSGASNLYTLRRITLPILKPAFLATFTLAIIRS
                     LAAFAVPSMVGMPGRIHVLTTHVYRIISVGWSPDYGRAAAIGLIVLAASIILVYLYRH
                     LIAESSQYVTVTGKGYRPIVVELGRARRPLFILCVVLFCFLVVIPLATLVYMSFLPYS
                     MVPSARAVSMMSLRNWRTVLANSVIIRALKNSTFLAVIGATLGILLSIFVAYVIVKVK
                     SRGSALIESLSFLSFSFPGMIIGVGFMWFLVRTPLYATIWGLLVAYVGTYLPYGVRPL
                     RSAFMQIDEELEESARVFGASFLRALKDIVVPLLAPGVVSAWILMACMFIRELSVSVV
                     LSRPGTEVLTVEIMKLANDGLWGQVAALGIIMIGLSTALVLVANMLRVPLSHVDQ"
     gene            complement(8961..9983)
                     /locus_tag="PLM74_01025"
     CDS             complement(8961..9983)
                     /locus_tag="PLM74_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HON41699.1"
                     /translation="MKTGIRNLIRSSVMCCIILAMVLGVAAGSEHPLLKEAKKEGKAI
                     FYANITGVEPVMQAFKSQYGVSAEYTRVSTAVYVPTVQTEFQAGRLAADVLQAPIPIL
                     QILKDAGVLAKYVSPVSEKYPKWSRDPDGVIQQFGIEAVAIIYNTELVKPQDVPTTYR
                     DLANPKWRDKIVMPDPITHATTITWLVGLKEHVFSTEAEWMEFLRGLAKNRPMFVASF
                     GPTPGPIETGEKVIGISMPKYIVTKAPAPLGWAKLNGEPLMGSARGIAVAKNAKRPNA
                     ARLFVDFWLSEASSRILAEQVGEWVLYEGIYPPIEGIKKADVIPLRELSDDEIQRWSE
                     VFKTIF"
     gene            complement(10153..10536)
                     /locus_tag="PLM74_01030"
     CDS             complement(10153..10536)
                     /locus_tag="PLM74_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HON41700.1"
                     /translation="MLKIIVWILVGLAVLVGGVIAFGLIGRRAGEALPIGSVDFSELA
                     DGEYMGQYKGGRWSNKVRVTVESGKVIGIEVVKDVLFSQPEVRRQTLESVVQAQSLQV
                     DVISGATITAKAYLKAVENALTGSD"
     gene            complement(10672..12057)
                     /locus_tag="PLM74_01035"
     CDS             complement(10672..12057)
                     /locus_tag="PLM74_01035"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HON41701.1"
                     /translation="MSTRSDGSPLASMAPKKKPQIITRALMVFLGAMILANIGHQMNQ
                     QMMPLYVQSLGANVQQVGLFFTIASIMPLTFQILGGFISDSIGRLQAIAIGSIAGLIG
                     YVMYVLAPSWQFMIVAQSISGIASCFVSPSFQAYIAEQSSEEMRARTFATVDSIYTVV
                     GVIGPSLGGLISERFGYRQMFAVSALLYSCAVVIRFAMAANASRGSSSRAGSAPGAAP
                     ERPTPAKFAQSMKAMISLVLAGGVITWLFISDGVSDIAFTLGGRLEPLYYSNIMGLSN
                     LQIGSLLSILNVTMMVMLPLGGWFADKAGERAGIAIGHVFFSAGYVLMLLGKRYAQFT
                     VVWVMYGIGASLMSPAYNSLISKAVPEKMRGTAYGLFSTSLGLISLPAPYIGGLMWRT
                     LGPRAPFVVPLLSSLILAPLLWIKLGRVNGKSNTAAPGAVDAATPEADIGSTTDRYLA
                     QLEGGPASADV"
     gene            complement(12214..12537)
                     /locus_tag="PLM74_01040"
     CDS             complement(12214..12537)
                     /locus_tag="PLM74_01040"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HON41702.1"
                     /translation="MDSVEGELRLVDIQRFLHRIGATRGILFGSRARGDYIASSDVDL
                     IIISDKFADVSFPWRMVFLHENWDLPLYLEGLPYTEDEFTRLKQTSSVVQDAVKHGIE
                     IWPDA"
     gene            complement(12507..12899)
                     /locus_tag="PLM74_01045"
     CDS             complement(12507..12899)
                     /locus_tag="PLM74_01045"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HON41703.1"
                     /translation="MREEAQFWAEDAKWDLECAQDMLDKARYNYAVWLSRQSVEKLLK
                     ACCLVILKSPVPRDHNLLTLGRSCFGDGMVEVNSQLTFLNPHYTTTRYVDAALGAPKD
                     IFDREFAEQALARAREVFEWIQSKANLG"
     gene            complement(13669..14763)
                     /locus_tag="PLM74_01050"
     CDS             complement(13669..14763)
                     /locus_tag="PLM74_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HON41704.1"
                     /translation="MPKLRVGIIGAGMAFERLHYPAYMKLADTYEIVAVCDPDQDKLS
                     KWQVSLGLSSRDLHTDWEAIVARDDVDVYDIMVPIELNYAVTEAVAKRLSGKRKAIIC
                     EKPLAGSFKEALSARELAQRYQVPILIAENYRYNEETNIVRQLVSDRRVGDVVYFLYN
                     HVMDFPQEMWKDAFPARDWRQYPEYPGGAITDSAVHDLAAIRHIFGAIDRLQAFGHPQ
                     DEEFSPYSAIVVNMLFSSGVVGQFSFFCSGKEAQLPAVGFRIFGSSGMIYLESRDAGV
                     THIAHNDGRIEGVPFEPQKGYYNELLNLANAMAGREPISVPPEMEYGDLKTVRDILKS
                     IQEQKIVSVDKTPDYTPDYSQTGVHVPFMH"
     gene            complement(14824..15636)
                     /locus_tag="PLM74_01055"
     CDS             complement(14824..15636)
                     /locus_tag="PLM74_01055"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HON41705.1"
                     /translation="MTIQGKRSRRRTVIQIIAAAAANSGLPALKFCPYPGLNCYACPL
                     ASFACPMGSFQNFLSLRRFPFAIAGFFITVGAVFGRAVCGWACPFGLFQDLLAGLGPR
                     RKMLRSNRWGWTRYVVLVGLAVVGAFAVGAPIFCKLCPAGTLGAGIPQVILRPSLRQM
                     IGPLYWIKIGLLAALVMVALFVKRPFCRFLCPLGAVYSPFNRVSLMRIRVDRSACSRC
                     NACQHVCPVDIAIYEDPDSDACVRCLECTRCPAVSVAYGPTKSEYPLHTAGN"
     gene            complement(15755..15943)
                     /locus_tag="PLM74_01060"
     CDS             complement(15755..15943)
                     /locus_tag="PLM74_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41706.1"
                     /translation="MARAMQASSGPGRVGWRGRGPGPYAVLVIAGIALMAVGVACGDF
                     GAILANARLICLSCIGIQ"
     gene            complement(15949..16512)
                     /locus_tag="PLM74_01065"
     CDS             complement(15949..16512)
                     /locus_tag="PLM74_01065"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HON41707.1"
                     /translation="MNRDAGNVRPLRRAALLIAGLAVAALLIWALPPGAERRAVAKST
                     EQLVGAVAPNFTLKTIDGKQIALRDQPRYVYVLDFWTVSCPPCHSVAPVLQDIHKKYG
                     FHGVRVVGVNLADSSAAIKRYMDSNGYTYIQTTDRGTVANAYGIYATPTVAVIDRKGI
                     VRDVMVGLASGWESRLESIVKTVAGEK"
     gene            16733..>17472
                     /locus_tag="PLM74_01070"
     CDS             16733..>17472
                     /locus_tag="PLM74_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41708.1"
                     /translation="MIKTTRTRGTPSRVMISRAVIENLLGASGSADSQQVDRAGLLVG
                     RLTSDANGINITVLDTVELPGVPLKAEPPGLLIALADNLRRKAQAAHVGKEIVGWFHT
                     HLGSGVAASGEDAMVHKSLSPLGAGVALIVDPVQHEASFFYMDEEALLPFPGYYMFDP
                     VGESREQSPSAPAAQASGSQTADELRKCVRLSPVTWLRRLRWLLQRPSNDTSLALRLA
                     SIALIIASIALAAQIWTPRSRVEQLAAQP"
BASE COUNT         3614 a         5206 c         4943 g         3709 t
ORIGIN      
        1 ctgactatag ggagcaccat ggcgttgcct atgctcatgc gcgaactcct gcccagcgtg
       61 aagccgcgcc gcaggaaaaa gacgccggcc catatcccaa ccgtgaaacc cagtacaccg
      121 aagacggcgt tgagatcccc gcccgcgagg cgaaggatca tcctgagagg gcaccccaga
      181 aagaccagcg cccctatgat cacgaagaag cccaggacaa accggagcac cggcgaagac
      241 ccgccccgcg cccggaattc gcctgctgcc agggcagcaa gcatagagcc tatgatgaag
      301 ccgggtacct ccgggcgaag atattggacc acctccgccc tatgcagccc tagcccgcct
      361 gcaatatccc ggatgaagca tgcaatacat attcccatgt ttgccgggtt tcctgtcgca
      421 gtcagagcga cgctgagagc gcctacgacg gcgccagcta tcaacactac tctacgctga
      481 ttcattgccg aaccagtccc ctcccgtttg tattcgcgtg cgtgcgcagt gtgtgcgtaa
      541 tttcacaatg ctcacaccgt aaagtatatg ctgaggcgcc agaccaccac aaatagcata
      601 tgttaatgga atgcatgtca gctatgagct attgatatgg gtagttgaca cccggcgcgg
      661 ccaaggcgat actaggcaac gggaggcgag cattccttgg gtatcgacgg cgctagtaag
      721 cgcatctatc tcgacaacgc ggcaacgtcc tatcctaagc ccggcgaagt ctgggagtcg
      781 atggcacgct acatgcacga catcggcgca agcccaggcc ggggaggcta tggaagcagc
      841 ctcgatgccg gccggctggt cttcgaagcg cgcgagaccg cggcccagct cttccacgcg
      901 cccggagccg agcaagtcgt attcaccccc aatgtcactt acgcgctcaa cttcgccata
      961 cacgggctgc tccgccgcgg cgaccacatt gtgaccacaa gcatggagca caactcagtg
     1021 atccgccccc taaggtcggc cggggagagc ataggggccg aggtcactgt ggtcaaatgc
     1081 gatgcgcagg gcaggctgga tcctcacgat gtgcgttgtg cgctcaggcc caacacccgg
     1141 atggtcgtga tgacccacgc gtctaatgtg tgtggtacgg tgatgcccat cgtcgaagtt
     1201 gccggcgtgg cgcacgaagc cggggtcttt atggtagtgg acgcagcgca gactgcaggg
     1261 gtcatcgaca ttgacctgag gtcgatggcc gttgacgtgt tggcgttcac cggccacaag
     1321 gggctctacg ggccgccggg aacgggcggg atggtactga gtgcgcgcgc ggcgacggag
     1381 atggcctctt ctatccaggg cggcacagga agccgatctg atgaggagtt ccagcccggg
     1441 ttcctgccgg acaagttcga gcccggcact cccaacacac ttggtatagc ggggcttagg
     1501 gccggaatcg gattcctgct ggaaaacgga gtcgatcaga taggcatgca cgaactgcgg
     1561 ctcgcaggcg agttcgcaaa ggctgcccgt gctattcccg ggattcgagt gttcggccta
     1621 tgggaaacgg gagacgaagc agcgatatgt gcgcgcgacc gcgtagcgac tgtgtcggtg
     1681 ggtttcgtat ctgcacaggc tgacccggca cgagcggcct ttcttcttga caaggagttc
     1741 ggcatcatgg ttaggtcagg actacactgc gccccgttag cgcacaggac gctcggaacc
     1801 tttcccgccg gcacactccg cttcagcttc ggctggttca atacgatgag cgacgttcgc
     1861 gatgccgccg gcgccctgtc atgcatagca gagcgatgct agctgtggaa atagtcgcag
     1921 tacgagttga tctggtgcgt ccagtctccc cgggtaacca tgtggtgatt gccgagcggg
     1981 cggctgagca gtatgcgcgc gtcctcaggg cggtcgaacc tcacagccag ctgagtgcgg
     2041 cataggtctt cgcgctcccc gcattcgacg agctggcccc ggactgcgta caagtcttgc
     2101 agttctttcc cgcctatgcg gacgatggta acagggcccg gttcaagctc tcccgtaatg
     2161 cctacaccca ggttcgactc gtagtgggat ctcagcgagt atctcgtgca catcgtcgta
     2221 ggaatggtgc agtgggtgaa caccactgtg ccggtccgca gatcgatgga cgacgggttg
     2281 gccaggaaac tgggctgaga tgtcaggtac cacatgaata gcatcgtaag acatgatgga
     2341 atatcggctt cgcacgtagc cggcactccc tcgtcgttaa ggcgcgcaag cgcatagcac
     2401 gcggtgttcg caagcggcgt gagcagggag aaacaatgca cggccacagc atccaggtgg
     2461 tactcctgca ctatccgcct gagccctagg tatatcctgg ccgatcccag catagcgttg
     2521 gcatcgggct caatgacccg ctccgccgct gcctgcatct gagccgctac atcctgtgcc
     2581 gcctctgcat cggcttcgcg tgagcagcgt accagttccc ccaaaggcaa atgcaccagt
     2641 tgcggccccc acacttccct cacggcgccc tccagagccc agggcgccat gacctcgata
     2701 tcccgcgatc caagtgacgc gattactgcc ctgcgcagcg acgaatacgt ctttgctagc
     2761 gtaacggtga gcgcgatatc ctcgcgccaa ccgcgactgc cctgcactat gatgcccgca
     2821 tgcccgacgg atctggcgag cgcgagtatc tccatggcag cggccagggc attgtcgccg
     2881 gggtgagcga gtatcgcaac agggccattc catcgagcga tgaccgacat gcccagttgt
     2941 tcagtgccgc cgctcaagac caagagcagc agagcatcgg accccgcgag ttccatggcc
     3001 gacactcgcg agtcgtgcca ttgaaactgg atgtccttgc tggcgccgag cactgcaagt
     3061 tctgctctgt agtgttcggc cctgtcagtc acctcgcgaa gatcgcgcca cgaggatgca
     3121 acctccacca cactgactcg catcggcgga tctaccccct tgtgtgcctg cttacccctg
     3181 actacattcg ataagctagc cgggaaatcc tacagtgcga ggtcgatatt cacaagaatg
     3241 tgcacagatt ccttcgagtg ttcatcgatg caggcaggag atgcgccgct caagttgaaa
     3301 ttgtaaacga ttggagttga ccgcgagcta gcctgcatgc aaggctgtgg ttggaggtgc
     3361 tcgagtgaga aacgcgaagg cgataatgat gctgtttctt cttgccgctc ttgtatgtgg
     3421 aatcgccgtg tcgactagcg ggtctgcagc cgacatgggg caggaatcgt tacccgctca
     3481 aacgctcacc caagccccgg cgctgactcg cgtggccata gaccaactcc tagatgcttt
     3541 cgtcccatct gccatggccc gcctgcacgt cccgggcgtc acttttgtcg ctgtgaagga
     3601 cggggagatc ctgaccatgc gcggattcgg atactccgac attgaggcgg gcagaggcgt
     3661 ggatccggct gcaacggtat ttcgcgctgc ttccgtgtcc aaagtcttca ctggaatggc
     3721 agtaatgcag ttggccgagc ggggcgtcgt tgacctagat gaggacgtga acacatatat
     3781 caagggcttc aggatccctg caacgtaccc cgagccaatt acactacgtc acctcctcac
     3841 tcatactgcg ggtctagatg agcggaacct cgggatgtcg gaattcggat cgcgcgtcta
     3901 ccccagatta ggcgaatacc tcgcagctga gttgccgccg cgcatacggc ctgccgggca
     3961 ggagatccag tacagcaatc acggagcggc cctggcagga tacgtagtag aatgtgccgc
     4021 cgaaatgccg ttcgccgatt acgtcgcaga taagatcctt gccccgctgg gcatggaaca
     4081 cagcgacttt cggctgacac ccgacattga acagcatctt tcagcgagtt acgagtggcg
     4141 caagggcaag tatgtgaacg tgccttacgt gcacataaac gcggcgccgg cgggggcgct
     4201 gatgaccacg gccgccgaca tggccaggtt catgatggcg aacctggcag gcggcgagct
     4261 tgaaggcgcg cgcgtgctag gggccgaata cgtggaagca atgcagacgc agcagttcac
     4321 gaatgatccc cgagtacccg gaatcggata cgcatggttc atgggccggc ggaacgggcg
     4381 tcgtgtggtt ttgcacggcg gcgatctatg gcagttctcg actcagctcc tgctagcgcc
     4441 ggacgagaac ttcggactgt ttgtgtcggg caatagctcg ggggctgcac ccttagccga
     4501 tgagctcgtc aaggctctgt tcgacacctt cttccctaag catgaagagc cggacgtatc
     4561 gggtgctgtt cagcccgcgg gagacgtaag cacgctcggg cctgctcatg tagcgggcaa
     4621 tccccgcaag ctggccggcg tctaccggac aaaccggcgc ccgttaacca cagccgacaa
     4681 agccatatcc ctgttgaccc agttccgagt cgccgccaat gatgatggca cactgaccct
     4741 ggctttcccg cccggatacg gaatgccaat ggcgacgtgg acgccggcag gtccggggct
     4801 cttccgcgac agggccagcg atgatctcat ggctttcgac cattggaaga ccgtggcagg
     4861 cgaatcgcga ccgtcgcgca tgtacatggg gatatgggcc tttgacagag tgcctgtcta
     4921 tgagacggct tcgtttacac tggcggcggt atcggcaata gccgtggtgt tcatctgggc
     4981 catactggcc tgggcattcg gccgcagagt gagcgggctc gccgctgcac tcggcctggt
     5041 gaacctggct gccatcgccg gcatcgccgg ctcgctcctc gcgatcccgg gctgggaact
     5101 cacgactgag gtgccgcaga tgaccagagc ggccctggcc ctcccgctgg caggcggcgc
     5161 gcttgcgctg gcgctagtgt ggcaaaacat aaggcgcatt gctgcagaaa gacaacgaca
     5221 gcgctggatc ttctataatc gaagggcgcg cagggggctg acagggctca tcctcccatg
     5281 gttcgtgatt gccgccgacg ggtgcttctt gtggttgctg cacacctgga acctgctggg
     5341 gtggaggttc tgacctgctc ccaaccttat catccagatc cgctactcgg agtcgagttc
     5401 atgtgccagg atctctttga ccttgagctc aagttcatca agatccagcc cggcttcgtc
     5461 cttgatactc ttccgtagca tgggacctac ttgctcgata tcatccagga ggtcctgtat
     5521 gagctcaagc gctgcagtgg cctccagcac ttgccttgga gtccggacca tgctcgccag
     5581 gatagagttc tctgtccgcg aggccaccag agggctgggc agttgctcga catagacctt
     5641 agtgcgtcga ccgggtcccc ggtcgtcttc tatagggacc agggctatga tcctgtctgc
     5701 cctaacgtac ttgccgtatc ccagaggaac gacggcgtct tccctcactt ccatcaggct
     5761 tcacgtcctc tcgactgtat cagcttctcc ggttatcgtg tccaggatag agcagccgaa
     5821 tcagcggcag tagtccggtt ccggtgcaag gctgcccgga gtcgtaatcg gaagctatca
     5881 cgcctatatg gtgcctacag ataggagatt cgctccgtgg caacgaatat cggttaggga
     5941 agaacaacta gtaccgggta tggttgccag aggatgcgtc tcggccctac agaggaaggc
     6001 cggccgaggc ggcgacgaac cagagaggag caattgtcat gcagggaatg gtgtcagtca
     6061 agaatctggt caaaacgttt gtgtccaggg aagagacggt gacggctctc aatagcgtaa
     6121 cgttatctac ccctcccaat aggattctga ctcttttagg gccttccggg tgcgggaaga
     6181 ccacactgct caggtgcata gccgggcttg aatctcccga ctcaggcgag atctccatcg
     6241 gcgaccaggt agtgtggtcg aaagccaagg gcatatcttt gccccctgag cgtcggggta
     6301 ttgggatggt gtttcagtct tatgcgattt ggcctcatat gactgtcttc ggcaacgtgg
     6361 cgtacccgct cgagattcgt agaacaccga agcacgaaat cgaggagcgg gtcaagaaga
     6421 tcctcgacct tgtgcaactg ggaggtttcg agaatcgact agcgaccaag ctcagtggcg
     6481 ggcagcagca gagggtggct ctcgccaggg cacttgtagc cgagcccaaa gtgattcttt
     6541 ttgacgaacc cctcagcaac ctggacgcga agctccgcga gagcacacgc caagaactca
     6601 gggacttcct cacccagcta gccatctccg ctatctatgt tactcacgat caggttgagg
     6661 ccctgacaat ctcggacgat attgcagtaa tgcgtggagg ctcaatagtt gagcgaggca
     6721 cgcctcagga tatttacctt cgctccaaca acccattcgt ggtcgacttt atcggcagag
     6781 ccaacttcct gagtgcgaca gtcaaaggtt atggcggcga aaaccatgcc atcgtatcct
     6841 gcgccttagg tgatattgtg tgtagcgccc cgtccggcct gggcgtcgga actgaagtga
     6901 cgatctacac tcgccccgaa tccttcgaga tggtgccggc ctcttcccct cggatggaga
     6961 acgagtttga aggcaccgtt acaagtcttc tgtttgcagg ggaagcgcac gaggcagaga
     7021 tctccgttgg tggggctcgt gtgcttgcaa agctcaaatc gcatatcccg ctcaagaagg
     7081 gtgacaaggt gcgactgcat atcgatcgtg agtggtgccg cgcccttaac cctgcagagg
     7141 aggcctgaca gtgctcaatc atcgatctag tcgtcgtttc ccgcttgtcg cgttcctcat
     7201 catcatcgta agcgcgctaa caatcggccc tgtcgtaatg ctcgtgatag gtagcttctc
     7261 tgagggaatc ggggcgattg ggaagtttac aaccggcaag tatgtcgcag cctacaccga
     7321 tccctcactt cccaaaacgc ttttgaacac aatcgttttt acgcttggga ctgcatgctt
     7381 ctcaacggct ctaggtggac ttctcgccta tctgagcgtc cggacggata tcccgttcaa
     7441 gggcctattc cgagtccttc cgatagtgcc aatgatgatt ccccacgttc tgttcgcagc
     7501 tgcgtggata gcgttgatta acccgaccaa tggcatgcta aacctgttcc tcaggaacct
     7561 gttcgggttc tcgagggggc cgttcaacat atactcattg gctggcatga tcttccttga
     7621 agggatgctt gacctgcctg tcacttacct tgtgatctcg cctgctatgt actcatttga
     7681 cacgacgcta gaggaggcct ctcgggtcag cggggcgtcg aacctctaca ctctgcgcag
     7741 aatcacgttg ccgatactga agcctgcctt cctggccaca ttcaccctgg cgattatccg
     7801 gagcctggct gcgttcgccg tcccgtccat ggtggggatg cccggtcgta tccatgtctt
     7861 aaccacccac gtataccgga taatctctgt aggttggtcg cctgactacg gcagggcggc
     7921 agcgatcggt ctgattgtgt tggcagcatc catcattctt gtctatctgt accggcacct
     7981 tattgctgag agctcccagt acgtgactgt tacaggtaag gggtaccgtc ctattgtagt
     8041 agagctcggc cgcgccaggc gtcccttgtt tattctgtgc gttgtgctct tctgcttcct
     8101 ggtagtaata cctctggcca ccctcgtgta catgtcattc ctgccatatt ccatggtgcc
     8161 cagtgcgcgc gctgtcagca tgatgagcct tcgcaactgg cgtaccgttc ttgcaaattc
     8221 cgtcataatc cgggcgctaa agaactccac ttttctggcc gtaatcggag cgactctcgg
     8281 catcttgctg tccatattcg ttgcctacgt tatcgtcaaa gttaaaagcc ggggaagcgc
     8341 gctcattgag tccctctcgt tcctgtcgtt ttcctttcca ggcatgatca tcggtgtggg
     8401 attcatgtgg ttcctggtga ggactcccct ctacgcgacc atttggggcc tcctcgtggc
     8461 ctatgtgggt acctacttgc cttatggcgt tcgccctctc cgaagcgcgt ttatgcagat
     8521 agacgaggag ctcgaggaat cggcgcgagt gttcggcgcg agtttcctac gggcgctgaa
     8581 agacattgta gtgcctcttc tcgcccctgg tgtcgtgtcg gcatggattc tgatggcctg
     8641 catgttcatc cgtgagttga gtgtttcggt ggtgctgtca cgcccgggca ctgaggtgct
     8701 tacagtagag atcatgaagc tggccaatga cggtctctgg ggtcaggtgg ctgctctagg
     8761 catcatcatg ataggcctct ctactgcgct tgtgcttgtc gcaaacatgc tgcgcgtccc
     8821 tctgtcccac gtagatcagt aggtcaatag gctgggacgc tagagcacgg gggctaccga
     8881 atcggaaacg caaacaaaac aggctgtctt ctgggaaaca cagaaggcag cctgttagtg
     8941 ctcgctggcg tgtatcatgt ctagaagatt gtcttgaaca cctctgacca gcgctggatc
     9001 tcgtcgtcag agagctcccg cagaggtatc acgtcagctt tcttgatgcc ctcaataggc
     9061 ggatagatgc cctcatacaa cacccactcg cccacttgtt cggcaaggat gcgtgaagaa
     9121 gcctctgaga gccagaaatc gacgaaaagc ctggcggcgt tgggcctctt ggcgttcttg
     9181 gcaactgcga ttccccgggc cgaccccatt aggggttcac cgttcagctt ggcccagcca
     9241 agtggagcag gggccttagt cacaatatac ttgggcatag agataccgat gaccttctcg
     9301 ccggtttcga taggcccggg agtaggcccg aaggaggcca cgaacatggg tctgttcttg
     9361 gccagacccc gcagaaactc catccactca gcctcagtag agaacacatg ctccttgaga
     9421 cccacaagcc atgtgattgt tgtcgcatgc gttatcgggt caggcatcac gatcttgtcg
     9481 cgccatttgg gattagcaag atcccggtac gtggtaggta catcctgcgg tttgaccagc
     9541 tcggtgttgt agatgatagc cacggcctca atgccgaatt gctggattac cccgtcggga
     9601 tcccttgacc atttggggta cttctcgctt accggtgaca cgtacttggc caaaacgccg
     9661 gcgtccttca gtatttgcag tatcgggatg ggcgcctgga gcacgtctgc ggctaatcgg
     9721 ccggcctgga actcagtctg gactgtaggg acgtacaccg ccgtggagac acgggtgtac
     9781 tctgccgata cgccgtactg ggacttgaag gcctgcataa ctggttctac tccagtgatg
     9841 ttcgcgtaga atatggcctt gccttccttt ttggcttcct tgagcagtgg atgttcgctg
     9901 cctgcggcga cgccgagcac catggccaga ataatgcagc acatcaccga cgaacgaatc
     9961 aggttcctta tccccgtttt catctctgct tctgcaccac ctttgattaa tgcctaagag
    10021 ggccttaacc aacagtttcg gcaggcgtca ggggtttcct gcctgtcttg ccaaacaacg
    10081 caggaacgcc gggggtgaac accttgccaa tcacacaccg acgttccggg atatcgttcg
    10141 cgccctgcag cgctaatccg aacctgttag cgcattctcg accgccttaa gataggcctt
    10201 ggcagtgata gtcgctccgc tgatgacgtc cacctggaga gactgagctt ggacgactga
    10261 ctcaagagtc tggcgcctca cctcgggctg gctgaacagg acatctttga caacttcgat
    10321 gcctatgacc ttacctgact ccaccgtgac tcgtaccttg ttcgaccaac gcccgccctt
    10381 gtactgcccc atgtactcgc catccgcaag ctcagagaag tcgacgcttc caattgggag
    10441 agcctcgcca gcccttctgc cgatcaagcc gaacgcaatc actccgccaa ccagcacagc
    10501 cagcccaaca aggatccaca cgataatctt cagcatgtca ccctctcctc cgttcatcat
    10561 cggtcgacgt ccgcggccgc gctgtcggcc gtcggggaag ttgatgggtg ctattcgcca
    10621 tctgttggct ggcacctcta catatttaga gtactgcctt gacgttcggt cctatacgtc
    10681 agcactagcc ggcccaccct caagttgcgc tagataccga tcggtcgtgc tacctatatc
    10741 ggcctctggg gtcgcagcgt cgactgcccc cggcgccgcg gtgttggact tgccgttcac
    10801 gcggccgagc ttgatccaga gcagcggcgc aagtatcagg ctcgacagca gaggtaccac
    10861 aaacggcgcc ctggggccga gcgttctcca catcaggcca ccgatgtacg gcgcaggcag
    10921 ggatatcagc cccaggctgg tggagaagag cccgtacgcc gtgccgcgca tcttctccgg
    10981 caccgccttg gatatcagtg agttgtacgc aggactcata agcgatgcgc cgatcccata
    11041 cataacccat acgacagtga actgcgcgta tctcttgccg agcaacatca gcacgtaacc
    11101 ggctgagaag aacacgtgcc ctatcgcaat gcccgcccgc tcacccgcct tgtcggcgaa
    11161 ccaccctcct aggggcaaca tcaccatcat ggtcacattc agaatcgata ggagcgaacc
    11221 gatctgtagg ttgcttaggc ccatgatgtt gctgtagtat agaggctcaa gtcgccctcc
    11281 cagagtaaat gcgatatccg atacgccgtc cgagatgaac agccaggtaa tcacgccgcc
    11341 ggccaggaca agcgagatca tggccttcat actctgggcg aacttcgcag gcgtgggacg
    11401 ctcgggggcc gcacctgggg ccgaccccgc tctgctcgaa gaccctcgac tggcgttagc
    11461 cgccatagca aaccggatta ccaccgcgca cgagtagagt agtgccgata cggcaaacat
    11521 ctggcgatac ccgaatcttt cggatatcag tccgccaagg ctagggccta taaccccgac
    11581 cacagtgtat atggaatcca ccgtggcaaa cgtgcgtgcg cgcatctcct cagatgactg
    11641 ctctgcaatg tatgcctgga agctgggcga cacaaagcaa cttgctattc ccgaaatgct
    11701 ctgcgccacg atcatgaact gccagcttgg ggccaaaacg tacattacgt atccgatgag
    11761 gccggctatg ctgccgatgg ctatggcctg gagccgacca attgagtccg agatgaatcc
    11821 gcccagtatc tgaaaggtca gcggcatgat ggaggcaata gtaaagaaca gccctacctg
    11881 ctgcacgttt gccccaagcg actgaacgta aagcggcatc atctgctggt tcatctgatg
    11941 cccgatgttg gcaaggatca tcgctcccaa gaataccatc aacgctctgg tgatgatctg
    12001 gggtttcttc ttcggcgcca tcgaggcgag cggcgaaccg tcgcttcggg tagacatgga
    12061 aataccctcc gtccgatcca ttcgtatgca tggcaagcat actgcaagct cctccggcgc
    12121 gcacaggcca tgtggttagt cttgactact ggcttcctgg gcggtatgga gcagcgtacg
    12181 ctcaggcggg ctgaggatgc tgaagtccgc aagtcacgcg tccggccaga tctcgatgcc
    12241 gtgctttacg gcatcttgca ccacgctgct cgtctgctta aggcgcgtga actcgtcttc
    12301 agtgtatgga aggccttcga ggtagagcgg aagatcccag ttttcatgga gaaacaccat
    12361 tcgccatggg aagctgacat cagcgaactt gtcactgatg atgatgaggt ccacatcgct
    12421 tgaagcaata tagtcgcccc gggcgcgcga cccgaacaga atgcctcgag ttgcccctat
    12481 cctgtgaaga aatctctgaa tatcaactag cctaagttcg ccttcgactg aatccactcg
    12541 aacacctccc gcgcccgggc taatgcctgc tcggcgaatt cccgatcgaa tatgtccttt
    12601 ggcgccccga gtgccgcatc gacgtatcgg gttgtcgtgt agtgcgggtt gagaaaagtc
    12661 agctgtgagt ttacctcgac catcccatcg ccaaagcacg atctgcctag agtcaggagg
    12721 ttatgatctc tcggaacagg ggacttaagt atgaccaagc aacatgcttt cagcagtttc
    12781 tccactgatt gcctggaaag ccatacggca tagttgtacc tcgccttatc cagcatgtcc
    12841 tgagcgcatt ccaagtccca tttggcatct tctgcccaga attgggcttc ttcgcgcatg
    12901 gcagtcaccc cttaggcgag catccaacag caggttgatt gtaccatgtg ggtagcaaac
    12961 gttcaactca cccccggtgc ggaacccggt gccggatttg gatcatctgt ttccgaggcc
    13021 cgagttgcag cctgttgtcg cgtagccgat tatccacaga gtccatttga cctgaccaga
    13081 ttctggctca tcctgctcgt tttgtggccg gttttctgag cgacctgctc ggatcatgcc
    13141 cggtttggca ggtgcgagtt gtcagaattc gggccggcaa cggcgtttcc gatttttgga
    13201 gtcaggcagc tagcaaatcc gcaggcaggg gcgggttaca caatatggct atgcgcgtga
    13261 cgctcccgag gacgcgggaa aaggctccaa ctgtctggtg ttccactagg aaacgccaga
    13321 cacggccgcc ccttccctcc ctcggtgctc aaacctgaca catcctgagc aggtgcgtca
    13381 aaaaaccagt cacatttctt acaggttcgc taattcgctg atatatgcgg gtttccctaa
    13441 tgacctgcgt aaggcccttt catctgttac tggcgtttcc gatcgtttcc gatgtgcgcg
    13501 tcggcggaga ggaagaaatg ccgagccttg agagccacaa ccgctacacg caccccgaat
    13561 atggacgaaa tatcgacgca gggcaggtcg ggcatagaac acatgccgtt tgttatcatg
    13621 ttggaggtag ttacaggcct ttgctgagga gcggcccggg tacagcgact aatgcatgaa
    13681 cggcacgtga acgccggttt ggctatagtc cggagtgtag tccggggtct tgtcgacgct
    13741 tactatcttc tgctcctgta tggacttgag gatatccctc acagtcttca ggtcgccgta
    13801 ttccatctcg ggaggcacgc tgatgggttc cctgccggcc atggcattgg ccaggttgag
    13861 cagttcgttg tagtagcctt tctgcggctc aaaaggaacg ccctcgatgc gcccatcatt
    13921 gtgtgcgatg tgtgtcactc ccgcgtccct gctctccaag tagatcatgc ccgaacttcc
    13981 gaagatccgg aaaccgaccg ctgggagctg cgcctctttg cccgagcaga agaagctgaa
    14041 ctgacccacg acgccgctgc taaacagcat attgacgacg atggcgctgt agggcgagaa
    14101 ctcctcgtcc tgcggatggc cgaaggcctg aagcctatcg atagcgccaa agatgtggcg
    14161 aatagcagcg aggtcgtgca cagcgctgtc ggttatggct ccgcctggat actccggata
    14221 ctgacgccag tcgcgagcag gaaaagcgtc cttccacatc tcctggggaa aatccatcac
    14281 gtgattatac aggaagtaca ccacatcgcc cacccgcctg tcggagacta gctgcctgac
    14341 gatgttggtc tcctcgttat agcggtagtt ctctgctatc aagatgggta cctggtagcg
    14401 ttgcgcgagc tcgcgcgctg aaagggcctc cttgaacgat ccggccaggg gtttttcgca
    14461 gatgatcgct ttcctcttgc ccgacagccg cttggctacg gcttccgtaa ccgcgtagtt
    14521 cagctctatc ggcaccatta tgtcgtacac atccacatcg tcacgggcga caatggcctc
    14581 ccagtcagta tggagatccc gcgatgaaag gccgaggctc acctgccact tgctcagctt
    14641 atcctgatcc ggatcgcata ctgccacgat ctcatacgta tccgcaagct tcatgtaggc
    14701 cgggtaatgc aggcgttcga acgccatacc cgccccgatg atgcccaccc tcaacttggg
    14761 catgaaaatc actcccttgc gaccagtgct gcgtcagtcg cactagatca actgcgctgc
    14821 tacctagttt cccgcagtgt gcaagggata ttcggatttc gtcggtccat acgcgacaga
    14881 aacggccggg catcgcgtgc actcgagaca ccgcacgcag gcgtctgaat cggggtcttc
    14941 atagatagca atatccaccg ggcaaacatg ctggcatgca ttgcacctgc tgcatgcgga
    15001 ccggtctact cgtatgcgca tcaagcttac cctgttaaat ggcgagtaca cagcacccag
    15061 cgggcagaga aaacgacaga acggcctctt cacgaagagc gcaaccataa ccagtgcggc
    15121 tagaagcccg atcttgatcc agtagagcgg accgatcatc tgccgaagag acggtctcag
    15181 tatcacctgt ggtatcccag cgcccagcgt gcccgcggga catagcttgc agaaaatggg
    15241 cgcccccacg gcgaacgcgc cgacaacggc cagcccgacc agtacaacgt agcgggtcca
    15301 cccccaacgg ttgctgcgta gcattttgcg tcgtggccca agccctgcca gtagatcctg
    15361 gaacaacccg aacggacacg cccacccgca cacggcccga ccaaacacag ctccgacagt
    15421 gatgaagaaa cccgcgatgg caaatgggaa tctccgaaga gacaggaagt tctgaaacga
    15481 acccatcgga catgcaaacg acgccaaggg gcatgcataa cagttcagcc cgggatacgg
    15541 gcagaacttc agagccggaa gaccggaatt ggccgcagcc gcggcaatga tctgtattac
    15601 agtgcgtcgc ctcgatctct tgccttgtat cgtcattact ctcactcgtt atccggcagt
    15661 cagcatttgc tgcctagtag cgtctgccgc ccgaccgtag tcgttcggtg caaggtgcag
    15721 gcgcgcagtg gacggtggcc aacgattgtc gactctactg aatgcctatg caagatagac
    15781 agataagccg ggcgttggcc agaatagcgc cgaaatcgcc gcacgcaacg cccactgcca
    15841 tgagcgcgat ccccgcgatg acgagcaccg cgtacggccc tgggcctcgc cctcgccatc
    15901 ccactcggcc tggcccggag cttgcctgca tggcccgcgc caaagccctc acttctcccc
    15961 tgcaaccgtc ttcacaatac tctcaagacg gctctcccac cccgaagcca acccaaccat
    16021 gacgtctcgc acaatgccct tgcggtcgat gacggccacg gtgggcgtag cgtagattcc
    16081 ataagcattg gcaacggttc ctctgtcagt agtctggatg taagtgtagc cattgctgtc
    16141 catgtacctc ttgatagccg ccgagctgtc cgcaaggttg acgcccacga cacgcactcc
    16201 gtggaagcca tacttcttgt ggatatcctg cagcacagga gcgacggagt gacacggtgg
    16261 acagctaacg gtccagaaat cgagcacata cacgtagcga ggctggtctc gaagcgcaat
    16321 ctgcttgcca tcgatggtct ttagtgtgaa gttcggcgca acagctccaa ccaattgctc
    16381 agtgctcttt gccaccgctc ggcgctctgc tcccggcggc agcgcccata tgagtagtgc
    16441 cgccacagcc agtccggcta ttaggagtgc cgcccgccgc agtgggcgca cgttgcctgc
    16501 atccctgttc attgatgtga tcctccaatt tcgccgtcgc cgctttgccc ggcccggcgg
    16561 ttctgtcaat tgcgtgacct attcgtcttg cgaccctttc tgtccttcct ccaacctgat
    16621 agtgaacagg agaactatca gcctgtgcct ccaatcagcg cgtcgcaagg aaaatccatc
    16681 aacaagtgcg aattatcctt taggtgtatc ccagggctgg atggtgatcg agttgatcaa
    16741 gacaacacga actcggggca cgccgtctag ggtgatgata agtcgggcag tgatcgagaa
    16801 cctgctcggc gcatctggca gcgcagattc gcagcaggtt gaccgcgccg ggcttctcgt
    16861 aggcaggctc acgagtgatg ctaatggaat caacatcacc gtgctcgaca cggtggagtt
    16921 gcctggagtt ccgctcaagg cggaaccccc ggggcttctg attgccctgg ccgacaacct
    16981 ccgccgaaag gctcaagccg ctcacgtagg caaggaaatc gtgggctggt ttcacacaca
    17041 cttgggctca ggtgtagcgg cgtccggcga ggatgccatg gtgcacaagt cgctctcgcc
    17101 gttgggagca ggggtcgcgc tgatcgtcga tcccgtgcag catgaggcct cgttcttcta
    17161 catggacgaa gaagcgctgc tgcccttccc ggggtactac atgttcgatc ctgtcggcga
    17221 atctcgagag caatcgccat ccgcgcccgc cgcacaggcg tcaggtagtc aaaccgctga
    17281 cgagctgcgc aagtgtgtca ggctctcgcc tgtgacttgg ctgcgcagac tacgctggct
    17341 gctccagcgc ccaagcaacg acacgtcgct ggccctgcgc ctcgcctcca tcgccctgat
    17401 catcgcctcg atcgccctgg cggcacagat ctggacgcca cgcagccggg tagagcagct
    17461 cgcagcccaa cc
//
LOCUS       DAOTAC010000012        16531 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_2903_length_16531_cov_4.511593, whole genome shotgun sequence.
ACCESSION   DAOTAC010000012 DAOTAC010000000
VERSION     DAOTAC010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 16531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16531
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2903_length_16531_cov_4.511593"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /gene="cgtA"
                     /locus_tag="PLM74_01075"
     CDS             <1..413
                     /gene="cgtA"
                     /locus_tag="PLM74_01075"
                     /inference="COORDINATES: protein motif:HMM:TIGR03595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HON41709.1"
                     /translation="PEGVPVYNISALTGDGVQALLYAVSARLAQLPPEQLPIVEGSER
                     EEPRRYTARGPRLRDVSISRDGEVFVVIGEGAERLVQRTDLDNEAALRRFYGMLTRAG
                     IVDALRDAGMGEGDTVRIGDTEFVFAESYNMERD"
     gene            445..1047
                     /gene="nadD"
                     /locus_tag="PLM74_01080"
     CDS             445..1047
                     /gene="nadD"
                     /locus_tag="PLM74_01080"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HON41710.1"
                     /translation="MQRIGIMGGTFDPVHYGHLVTAEEAYHRFHLDKVVFVPSAHPPH
                     KLDKVITSPAHRYMMTVLATMSNPHFEVSSIEIDRPGPSYAVDTVAAFRRDYPNDTAL
                     YFITGADAILEMLGWKDSTTLLRMCDFVAATRPGYFIADGKIAEIFGENAPRVHFFEV
                     PALAISSTDIRARVRQARPVRYLLPDIVISYIERAGLYVV"
     gene            1110..1367
                     /locus_tag="PLM74_01085"
     CDS             1110..1367
                     /locus_tag="PLM74_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013985596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HON41711.1"
                     /translation="MSKTLYVGNLPWAVTDDQLQNVFSPYAEVIAARVITDRETGRSR
                     GFGFVEVATDDVDPIIRTLNGTSLGGREISVNEAKDRQMRM"
     gene            1471..2085
                     /gene="yqeK"
                     /locus_tag="PLM74_01090"
     CDS             1471..2085
                     /gene="yqeK"
                     /locus_tag="PLM74_01090"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR00488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HON41712.1"
                     /translation="MDGEVLGQIRTRLQMAIPHSRYEHSRRVEEEAARLAARFGADLE
                     VCATAGLLHDCARDLPLDRLIALWHRLGPDRKGPDPVTFSSALLHGPVGALIACEEYG
                     VRDPRVLDAISRHTVGARGMTVEDKVVCLADYIEPGRRFPGVDSIRNLAQHDLDLALI
                     CALGATIKHLVDSGALIDADIVLSRNDLLRKSTMMPKVGDTSAR"
     gene            2066..2485
                     /gene="rsfS"
                     /locus_tag="PLM74_01095"
     CDS             2066..2485
                     /gene="rsfS"
                     /locus_tag="PLM74_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HON41713.1"
                     /translation="MILLHVNTDIRPSDLPSLPHAMLAVSVALEKKAEDVVLLDISSV
                     SIMADFFMICTVRTDTHARAVRDSVTQALERAGLNLRRREGSDASGWVLLDWGDIIVH
                     IFGPAEREFYSLEKLWGDARVCCYDTEAPSVEHRPAE"
     gene            2777..2852
                     /locus_tag="PLM74_01100"
     tRNA            2777..2852
                     /locus_tag="PLM74_01100"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2810..2812,aa:Ala,seq:ggc)
     gene            3010..5526
                     /gene="leuS"
                     /locus_tag="PLM74_01105"
     CDS             3010..5526
                     /gene="leuS"
                     /locus_tag="PLM74_01105"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HON41714.1"
                     /translation="MRDKYYFEDVEPKWQEQWEKEGLYRVDETPDRPKFYQLEMFPYP
                     SGRIHMGHVRNYCIGDVMARYRRMNGWNVLHPMGWDAFGLPAENAAIQHGANPAVWTR
                     ENIAHMRTQIKRMGYSYDWAREVATCDPAYYKWTQWLFIQLFKNGLACRKSQKVNWCP
                     SCATVLANEQVVDGACERCDTPVIRRQLEQWFFRITDYAERLLYDLDTMPGWPEHVKT
                     MQRNWIGRSEGMDLDFTVAGTGEKLTVFTTRHDTVYGVTYLVIAAEHPLVEHLIAGRP
                     NEDELRRFVSEIISQDDIVRTAEDAEKVGMFTGGYAINPASGEQVPIWIANYVLMDYG
                     TGAVMGVPAHDQRDLEFARKYGLPVKAVISPPGGSINGDTMESAYVEDGVQINSGQFD
                     GMPSGEAKPAMARYLESLGKGRPTVHYKLRDWLISRQRFWGAPIPIIYCEHCGQVPVP
                     DADLPVMLPTGVKFMPTGQSPLLDCPEFVNTTCPVCGGPGRRETDTTDTFVCSSWYFL
                     RYTSPQCHDHAWDRAKADYWMPVDQYIGGVEHAVMHLMYARFFTMVMSDLGLIGVDEP
                     FKNLITQGMVLLGGHKMSKSKGNTVDPDDIVGKYGADTARLFTLFASPIERDLEWSDE
                     GVEGCWRFLQRVWRLVYQCVEGPGCNQSAEGPAAPAVGTMSATSDCARRVRRAAHAAL
                     KKVTEDIGARNSLNTAIAAIMEYVNALYSLKDAVAGQEGGDAAMAEAIDFLIVMLAPF
                     APHISEELWHAVGHGESVHTQPWPKYDPEALAVESVEIAVQVNGKVRDRISVPVDVSG
                     DAALAAAMASPRVSAAVGQASVKKTILVPGKLLNIVVETA"
     gene            5655..6440
                     /locus_tag="PLM74_01110"
     CDS             5655..6440
                     /locus_tag="PLM74_01110"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HON41715.1"
                     /translation="MQAFGLRRAGPILAGVLLIGLAITLASPARRPRVVVFEGSSEAS
                     PMQGQGTSDLPEPSAPDQSMPRAEPAPMPQQAVTVHVAGAVAKPGVYSLNPGSRVADA
                     LDAAGGASKGADTDCINLALPVRDGEQVYVPLRQASAGAGNAGVVSPEGGGQPQPRQS
                     AVASIPPNRADITRSAPDSSPSSDSADARVSSNASHTGLININTASVEELQRLPGIGP
                     VLAQRIIDYRSANGPFPAVDDLVGVSGIGQAKLGRIAPLATAR"
     gene            6444..8972
                     /locus_tag="PLM74_01115"
     CDS             6444..8972
                     /locus_tag="PLM74_01115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HON41716.1"
                     /translation="MALSGTDFALSGGARWSPVVVPAFASSLAVGLLCGYWGIPAAVW
                     ICAWVVWAAVALVNSLALRLSPEYRARMILVAAMVGAASIGALGIGRIDSLAGVSVAR
                     FAGRDVVLMGTVISSRAGDRSPEAVIAARSVDDHGSLHHTAGKVRVFFTPGDASGGLA
                     ESLRCGDIIESRVRLELASRPMNPGEPDLRAVFLQQGIHALANIDSRSARVVGRRRLN
                     PVECIALWVRQAIIVAARATLAPGPCETFLAMALGDSRELDYGTLESFRRAGVSHLLA
                     ASGLHVSLVVGIAIQAAAAAGMSSRHSPLLGFSIAGVYAVVAGLRPSIVRAWLMFGLS
                     AASRACGRRVSALHVVCLSATAQLILNPLLLWNAGFQMSYLAIVALFYLAPCFARIVP
                     DRWPAPAASSLRTLLASTAVGTTLLPIVANMTLEISLIGPITNLVAVPLGLVAMTAGL
                     GGCVTWHVWPWLGSVMNAGAEAVLTVLASFVRAMASMPFSSVPIRKLSEWEIAAYYAL
                     LACTCAIGSDVFRLHRFRRAVGRIAKRPAAVMCSAAIVLLLAFRPAPFEVVVMSVGQG
                     DAVFISAPEGVNILVDAGAAWAGDKYVVPYLERRGVRRLDLLVITHEHADHAGGAAAV
                     ARSVRTECVAVPGCSSGQAWEAILGGLETACGGRSPRMVGLSQGHVIRAGGVTLEVLN
                     PPAKSGVAVGGHMADANDDSVVLRLVYRDFSMLLCADAGERFERRILDGPLQATSLAS
                     QVIKIGHHGSSGASSSRFLETVSSSLAIVSVGRNGYGHPSPAAVHRILDAGAEIRQTD
                     TCGAVSVSITSRGAAQAMRRWAGYVARDMRGVWARAPMFRRLQI"
     gene            9149..10414
                     /locus_tag="PLM74_01120"
     CDS             9149..10414
                     /locus_tag="PLM74_01120"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HON41717.1"
                     /translation="MKRFAAILLIAAFIVTTLAIGPTLAQQKTTITFWHAMGAQLGKT
                     IDMLVEQFNNENPDVFVKAEYQGNYGALSQKLVGSLVARKPPTVAQVYGNWAAEYITS
                     KELVPVEKFIKGPNGLSTLEIDDIWEGLRAGCVFDGVWYTMPFNKSVYVLVYNETALK
                     EAGIAKPPATWQELLTAARALTVKEGDKIVRYGFGLRPNVDYFACFFFTAGGTWLDAG
                     GKVNVNSPEGTKALQYMADLLSKYKVSYYVPGYLDADFGAGKVAMYLTTSPGLSYTDQ
                     SVAGKFEWSASPLPYMEPKFKATPVAGTDLAIFAKATEKEQQAAWKFVKWLVEPRQTA
                     KWAIGTSYIPVRKSAMHLEIMKQWFAQHPREEQSLQQLRYIKYDPNISAWTNIRGDIS
                     EAVEKVFLGKATAKEALDAAVVKANARLK"
     gene            10512..12656
                     /locus_tag="PLM74_01125"
     CDS             10512..12656
                     /locus_tag="PLM74_01125"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HON41718.1"
                     /translation="MRKPFRVARTRFLPLLFAVLAATIAVALALSVPQAASAEALAGN
                     YNYYFGNLHSHTSYSDGVLTPSDAFRHAREAARMDFHAVTDHGYYMQEATNLHHWFLA
                     HEEADEFYEPGRFVTLVGYEWTFTDGHMNGFATPRAASRDTQRDLTAFLEFLAEYDGI
                     GVFNHPSYDIQPNWNDFEYRGLGDKQVSLIEVGSGPYRHNVRNERAYIRALDRGWHVG
                     AVSSQDNHRPDWGTAAPTRTGLIASELTRESVLSAMRDMRTYATEDSNVRMFFASGNS
                     IMGDEIVLAPDVVMSGKSVEFRVYVADPDDTDYLELVQIVSNGGEVVWESRPGRLEPR
                     GAGQGGQAGGVGVAVTAYDTVVSLTPRSSYNWYYVRAVQSDGDVIVTSPIWVTTGSGV
                     AVCDFACVDTVVRAGVSARIRAEIVNRNEQAIRAVTAVLYETAPSGRRAVSSRRVDLA
                     GWAGTDIWFDWIPGAPGETGLELELIIPGHEAPLVFPGAPALVRRADITRVVIDEGHN
                     NRYTGYMDGFVTLLAASDYSPRIGEEPIGPDMLAETDILVVNVPEIGFALTPTSFEQD
                     ELEAIAAFVEQGGFLLLAGWSPSPDGTRTCEQLNVLLQLMHAGMGFADDELQADGSSS
                     VCAYWGSSEATGGPIYASEACSVSIEGSGATVAVFARAADGRVFAAGQSVGSGRVVCL
                     GAPVYSSLDLKREFSKNSDFTLALMVWLVKGE"
     gene            12665..13567
                     /locus_tag="PLM74_01130"
     CDS             12665..13567
                     /locus_tag="PLM74_01130"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HON41719.1"
                     /translation="MKPSRSRKYARDTAAAYLYLAPALIVLIVFHFFPAFYALYLSLF
                     DTNLFGRWNWVGLGNYAELLHDQDFLRSLANTGKYVLGTVPVGIALALAIAGLLNQRI
                     RGLAAYRTAFFLPYVTPVVAISIVWTWIYKEDSAGMLNAVLSWFGISPQAWLLDPKWA
                     MFALCLMSIWRHLGYNVVIFLAGLQNIPAEYYEAAEIDGASGWAMFSKITWPLLSPTT
                     YFITIISIIGSFQVFTQVYVLWPSALGGPLGTTRVVVKYLYDMAWGSYQFGYASAIGY
                     MLFAIIFVLTLVQRNVAGRYVHYQ"
     gene            13597..14430
                     /locus_tag="PLM74_01135"
     CDS             13597..14430
                     /locus_tag="PLM74_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007083847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HON41720.1"
                     /translation="MSKTRKTAHMTLVYVLLTVGAVMMLMPFYYMLSTSVKAPEELYV
                     NRLVWFPSKIVWKNYADAWNEEPYFPRYFFNSAFIATVTTALQLLTSALAAYAFSNID
                     FWGKETVFMILLGTMMIPSQVTLVPNYAIIARLGWIDTYKALIVPFTASVFGIFMMRQ
                     FFQTIPMEMWDAAQIDGCSRFRYLWAVMVPLARPIFVTSGLFTFIGGWNSFLWPLIVT
                     NSDHLRTIQVGLSRFNQEFGTRPDLLMAASTLAIAPLVALFFVAQNQFIQGIARTGLK
                     S"
     gene            14446..15483
                     /gene="holA"
                     /locus_tag="PLM74_01140"
     CDS             14446..15483
                     /gene="holA"
                     /locus_tag="PLM74_01140"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HON41721.1"
                     /translation="MRPDVAATMKKGTTAPIYIMYGEDAYSRGRNVDELVERLTSARG
                     GADLNAFDAAESSVDDIASAVMTAPMFASARVVIVRRFEEMSTDDQARLIRTLAPGKR
                     PADLRVPDETQVIIVSSSKTVPRKEVNAAGPHVAAFEFKPAYARDARAFVFERAKELE
                     VRIDPDAASLLLELAGLDYVAISQELEKVVTYLGPSGRRIAVDDIRAAVGRSSSQTVF
                     DFCDAVIDGNSTKALSALADLIRSERQVLAILGTLATKLRRVMEARVMLDGGSTQDEA
                     ATALVGGNPKVKWKEARTAAQASRLSQMQIAAMLSRIARADLDIKTGLMSESLAIEVL
                     ISDLCVRQKLQ"
     gene            complement(15562..15834)
                     /gene="rpsT"
                     /locus_tag="PLM74_01145"
     CDS             complement(15562..15834)
                     /gene="rpsT"
                     /locus_tag="PLM74_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HON41722.1"
                     /translation="MPNIKSAKKRVKTIKIRTARNTAIKSSVRTAIRRFNEALGQAGP
                     QGSVDELNRAFSTVDRAAKKGVIHKNQAARRKSRLARRANKAAQVD"
     gene            16116..>16531
                     /gene="gpr"
                     /locus_tag="PLM74_01150"
     CDS             16116..>16531
                     /gene="gpr"
                     /locus_tag="PLM74_01150"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: protein motif:HMM:NF015383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HON41723.1"
                     /translation="MRTDLAVEAHEVLRQGMGPEIPGVSTRTDETDFGSITRVAITTP
                     QAARALGKAMGNYITIDAPDLPLRDRELESEVSQALARELVGLIRTSLNNVDFWAVPD
                     FTTLICGLGNWNATPDAIGPLVAGKVMVTRHVYSMTP"
BASE COUNT         3231 a         4679 c         5135 g         3486 t
ORIGIN      
        1 tgcctgaagg tgtgccggtg tacaacatat ccgccctgac gggcgatggc gttcaggccc
       61 tgctttacgc ggtgtccgca aggcttgcgc agttgccgcc tgaacagctt ccgattgtgg
      121 aaggcagcga gcgcgaggag cctcggcgct atactgcacg cggccctcgc ctgagagatg
      181 tgtccatctc gcgtgatggt gaagtcttcg tcgtgattgg cgaaggagcc gaacgtctag
      241 ttcagcgtac cgatcttgat aacgaggcgg ctctcagaag attttacggt atgctcacgc
      301 gcgcgggcat tgtagacgct ctgcgcgacg cggggatggg cgagggggat accgttcgca
      361 tcggcgacac agaattcgtg ttcgccgagt cttacaacat ggaacgcgac tagctacact
      421 agttgcgcag gattaggtgg ttgcatgcag cgcataggga tcatgggcgg cactttcgat
      481 ccggtacatt acgggcattt agtcacagct gaagaggcat accatcgttt tcacctcgac
      541 aaggtggtgt tcgttccgtc tgcgcatcct cctcacaagc tcgacaaggt catcacgtct
      601 cccgcacatc gttacatgat gacggtgctg gctacgatga gcaatcccca ttttgaggta
      661 tcgtcaatcg agattgacag gcctggccca tcctacgctg ttgacaccgt tgctgccttc
      721 aggagggatt acccgaacga tacagcgctc tacttcatta ccggagcaga cgcgatcctg
      781 gaaatgctgg ggtggaagga ttcgaccaca ctccttcgta tgtgcgattt cgtggcggca
      841 acacgtcctg gatacttcat agccgacggc aaaatcgccg aaatattcgg agagaatgcg
      901 ccgagagtcc atttctttga agtgcccgcc cttgccattt cgtccaccga tatccgagcg
      961 cgcgttcgcc aagcacgccc tgtgagatac cttttgcccg acatcgtcat cagctacatc
     1021 gagagagccg ggctctatgt cgtgtgacgg cgcctgaata tcccagcctg cttcgcgaga
     1081 gactatgacc gatggagcga ggtgctagga tgagcaagac actatacgtg gggaacttgc
     1141 cgtgggcggt cacagatgat caacttcaga acgttttctc gccgtacgct gaggtgatag
     1201 ctgccagggt aatcaccgac cgcgaaacgg gtcggtcgcg cgggttcggg tttgtagagg
     1261 tggccactga cgacgtggat ccgattatcc ggacgctcaa tgggacatca ttgggcggac
     1321 gggaaattag cgtgaacgag gccaaagaca ggcagatgag aatgtagcgg ccgggttcct
     1381 gagccagcgt cgccccccgg gtttcacccg ttgggggcga tgtgctatta tggatgcagg
     1441 ctttgtgtga atcactggag ggcgtgctcg gtggatggtg aagtgctcgg gcagatcaga
     1501 acccgacttc agatggccat tcctcattcc agatacgaac atagcaggcg cgttgaggag
     1561 gaagcagcac gcctggcggc gagattcggg gctgacttgg aggtgtgcgc cactgccggg
     1621 cttctgcacg actgcgctcg cgacctgcct ctggatcgtc tgatcgctct ctggcatagg
     1681 cttgggcctg atcgaaaagg ccctgatccc gtcacgttca gctccgcgct ccttcacggg
     1741 ccggttgggg ctttaatcgc ctgcgaggag tacggggtgc gcgatcctcg cgtgctcgac
     1801 gccatcagcc gtcatacggt gggagctcga ggcatgaccg ttgaggacaa agtggtttgc
     1861 ctggccgatt acatagagcc gggaaggcgt tttccgggcg tggattccat caggaacctc
     1921 gcgcagcatg acctggattt ggcgctcatc tgcgcgctcg gcgcaaccat aaagcatctg
     1981 gtggactccg gcgcccttat agatgcggac atagtattga gcaggaatga tctgctgcgc
     2041 aagtcaacaa tgatgcctaa ggttggtgat acttctgcac gttaacaccg atatacgtcc
     2101 aagcgatctc ccgtcacttc cccatgcgat gcttgccgtc tcggtagcat tggagaagaa
     2161 agccgaggat gttgtccttc tggacatctc ctcagtctcc atcatggccg acttcttcat
     2221 gatctgcaca gtccgcaccg atacgcacgc tcgcgctgtc agggactccg taacgcaagc
     2281 tctggaacgc gccggtctga acctgcgaag gcgcgagggt tctgacgcgt cgggctgggt
     2341 cttgttggac tggggcgaca tcatagtcca catattcggc ccagccgaga gggagttcta
     2401 cagcctggag aagctgtggg gagacgctcg ggtctgctgc tacgatacgg aggccccctc
     2461 ggttgagcat cgcccggcgg agtaatctga ccgacgcgat caagcgggca cataacaatg
     2521 gcgcgttcat caaatggcgc gttcatcgct tgacttaccg ccgccctggg gctataatgg
     2581 gatctgcttg ataattcacg atggaaacgc gatgaagagg agcattaggt gctgaatggc
     2641 tttgagagag tcgggggtgg gtgcgacccg acagccggaa ggcatcgaac tcgcctccaa
     2701 gcggcgcggg tgaacctgag taacctgcga cggtcggccc cgttacaggc cgagaggcgt
     2761 ccgtgatctg ttgcgtgggg ctgtagctca gctgggagag cgcttgaatg gcattcaaga
     2821 ggtcgagggt tcgatcccct tcagctccac cattcttctt ttgttcgccc aggtcacgta
     2881 cgtgcaacag ggtggtaccg caagtaagcc ttgcccctgg cagtctatct gtcaggggtt
     2941 ttttgttttc catagcttgt ttacttcgca agctcatgtc gcatgctttc atctgaggga
     3001 ggactactga tgcgcgacaa gtactacttc gaagacgtgg agcccaaatg gcaggagcag
     3061 tgggagaagg aaggtctcta tcgcgttgac gagaccccag ataggcctaa gttctaccag
     3121 ctggagatgt tcccgtatcc gtcgggccgc attcatatgg gccatgtgcg gaactactgc
     3181 ataggcgatg tgatggcaag atatcgtcgg atgaacggct ggaatgtgct gcaccccatg
     3241 ggttgggatg cctttggcct cccggctgaa aatgcggcga ttcagcacgg cgccaacccg
     3301 gcagtgtgga cgagggagaa catcgcccac atgagaacgc agatcaagcg catgggctac
     3361 agctacgact gggcgcgcga ggttgcgacg tgcgaccctg cgtactacaa gtggacacag
     3421 tggcttttca tacagctgtt caagaacggc ctggcttgtc ggaagtcgca gaaagtgaac
     3481 tggtgcccat catgcgccac tgtgctcgcc aatgagcagg tggtagatgg ggcgtgtgag
     3541 cgatgcgaca ctcccgtcat tcggcgtcag ttggagcaat ggttcttccg tataaccgat
     3601 tatgccgagc gtctgctgta cgatctggac accatgccgg gctggccgga gcatgtcaag
     3661 accatgcagc gcaactggat cggccggagc gagggtatgg atctcgactt cacagtcgcc
     3721 ggcacaggcg aaaagctcac cgtgttcacg acacgccacg atactgtata cggcgtgacc
     3781 tacctggtga tagccgcaga gcatccattg gtggaacacc taattgcagg aagacccaac
     3841 gaggacgagc tcaggcggtt tgtatcggag atcatatccc aagacgatat tgtgcggacg
     3901 gcagaggacg ccgagaaggt gggaatgttc acaggcggat atgcgattaa ccccgcttca
     3961 ggtgagcagg tgcccatctg gatcgcaaac tacgtgctca tggactacgg cacaggcgcg
     4021 gtgatgggcg tacccgccca cgaccagcgc gacctggaat tcgcgaggaa gtacggcttg
     4081 ccggtaaagg cagtcataag cccgcccggc ggaagcatca acggcgacac catggagtct
     4141 gcctatgtgg aagacggggt gcagatcaac tcgggtcagt tcgacggcat gccgtccggc
     4201 gaggctaagc cggccatggc gcgctatctg gagtcattgg gcaagggcag gcctacagtc
     4261 cattacaaac tgcgcgattg gctcatttcc cggcagcggt tctggggcgc gcccattccg
     4321 atcatatact gcgagcattg cggacaggtg cccgtgcccg atgccgatct gccggtgatg
     4381 ctgccgaccg gggtgaagtt catgcccacc ggccagtcgc cgctgctgga ctgtccggag
     4441 ttcgttaata ctacgtgccc tgtgtgcggt ggaccgggcc gccgagagac ggataccacg
     4501 gatacgtttg tctgttcgtc atggtatttc ctgaggtata cgtcgcccca gtgccacgat
     4561 catgcctggg atagggcgaa ggcagactac tggatgccgg tggaccaata catcggaggc
     4621 gtcgagcatg ccgtcatgca cctgatgtac gccaggttct tcaccatggt catgagcgat
     4681 ctgggcctca tcggtgtgga tgagccgttc aagaacctga taacccaggg tatggtgctt
     4741 ctaggcgggc ataagatgtc gaaatccaag ggcaacaccg tcgatcccga cgatatcgtc
     4801 ggcaagtacg gagccgacac agcacgcctg tttacactgt ttgcgtcgcc aatcgagaga
     4861 gatctcgaat ggagcgatga gggtgtagag ggatgctggc ggttcctgca gcgagtatgg
     4921 agactggtct accagtgtgt cgaaggcccg ggatgcaatc agtcagccga aggaccggcc
     4981 gcccctgcgg tcggcacgat gtcggcaact agcgattgtg cccggcgtgt gcgcagagcc
     5041 gcgcatgctg cgcttaagaa agttactgag gatatcggcg ctcgcaacag tctgaacacc
     5101 gcgatcgctg cgataatgga atacgtgaac gctctataca gcctgaagga cgctgtggcc
     5161 ggacaagagg gcggggatgc ggcgatggcg gaggccatag acttcctcat cgtgatgcta
     5221 gccccattcg ctcctcacat ctcagaggag ctgtggcacg ctgttggtca cggcgaaagc
     5281 gtgcacaccc aaccctggcc caagtacgat ccagaggcgc ttgcagtcga gagcgtggag
     5341 attgctgtcc aagtcaacgg caaggtgcgt gacaggatat cagtgcctgt cgacgtgagt
     5401 ggggatgcag ctctagctgc ggcgatggca agccctcggg tgtcggctgc agtagggcag
     5461 gcgtccgtca agaagaccat actggttccg ggcaagctgc tgaatatcgt agtcgagaca
     5521 gcgtagcctg ggcatcgcgg aatcacccgt atgaatcacc caaaggcatg acgcgccggg
     5581 cccttcgcct gtgttagcat acaggcaagg cccggcgttc caccacagtg ccggcgcgtc
     5641 agggagggct ggctatgcaa gcattcggcc tacgaagggc aggtccgatt cttgcaggtg
     5701 tgctgttgat cggcctggcg attactctgg ccagcccggc gcgccggcct cgagtggtgg
     5761 tttttgaggg ctcttccgaa gcaagcccaa tgcaggggca gggcacgtcc gatctgccgg
     5821 agccgagtgc gccggatcaa tcgatgccac gcgccgaacc ggctcccatg cctcagcagg
     5881 ccgtcactgt ccacgtggcc ggagcagtgg cgaaacccgg cgtatactct ctcaaccctg
     5941 ggtcgcgggt tgcagacgcg cttgacgcgg ccggtggagc gtcgaagggt gccgacactg
     6001 attgcatcaa ccttgctctg cctgtccgcg acggggaaca ggtgtatgtg ccgttaaggc
     6061 aagcgagcgc cggtgccggc aatgccggtg tggtttcgcc agaaggcggt ggtcagccgc
     6121 agccccgcca gtctgctgta gccagcattc cacctaaccg tgccgacatt acacggtcgg
     6181 ctccggattc gtctccctca agtgattcgg ccgatgcgcg ggtttcttcg aacgcttccc
     6241 acacaggctt gatcaacatc aacactgcgt ctgtagagga attgcagcgt ctgcccggaa
     6301 ttggcccggt gctggcccag cgcattatcg attatcgatc tgccaacggc cctttccccg
     6361 ctgtcgacga cctcgtgggc gtgtcgggca taggccaggc caagctaggc agaattgcgc
     6421 ccttggcaac ggcgagatag ccaatggccc tctcgggcac ggatttcgct ctgtcaggcg
     6481 gtgcgcgctg gtcgcccgta gttgtccctg cgttcgcgag ctctctggcc gtgggtctac
     6541 tctgcggata ctgggggatt cccgccgctg tgtggatctg cgcatgggtg gtctgggccg
     6601 cggtcgcgct tgtgaatagt cttgcgctca ggctgtcgcc tgagtaccgc gcccgaatga
     6661 tattggtcgc agcgatggtg ggcgcggcca gcatcggggc tttgggcatc ggccgcatcg
     6721 attcgctggc gggcgtgtcg gtggcgagat tcgccgggcg cgatgtcgta ctcatgggca
     6781 ccgtgatatc gagtcgtgca ggtgaccgct caccggaggc ggtgattgcc gcgcgatccg
     6841 ttgacgatca cggatcactt catcacacgg cgggcaaagt cagagtgttt ttcacgcccg
     6901 gcgatgcctc tggcgggttg gcggagagcc tgcgttgcgg cgacattatt gaaagccggg
     6961 tccggttgga actggcttcc cgcccgatga acccgggcga gcccgacctc agggccgtct
     7021 ttcttcagca ggggattcat gcactggcca acatcgattc ccggtcggcg cgtgttgtag
     7081 gcaggcgacg gctcaacccc gtcgaatgca tcgcgctgtg ggtgcgacag gcgatcatcg
     7141 tcgctgcccg tgccacactt gctccggggc catgcgaaac ctttttggcg atggcgttag
     7201 gcgattcgcg cgaacttgac tatggcacgc tggaaagctt caggagggca ggagtatcgc
     7261 atcttctggc ggcgtctggg ctgcatgtga gtttagtcgt cggcatcgcg attcaggccg
     7321 ctgccgcggc aggcatgtcg tcgcgtcact cgcctctgtt gggattctca atcgcaggcg
     7381 tgtacgccgt agtggcggga ttgcggccat cgatagtacg cgcgtggctg atgttcggcc
     7441 tgtcagctgc atcgcgtgcc tgcggtcgtc gcgtgagcgc tctgcacgta gtgtgcctgt
     7501 cggccacagc ccagctcata ttgaacccgc tgcttctgtg gaatgcagga ttccaaatgt
     7561 cgtacctggc gattgtcgcc ctattctacc ttgctccatg cttcgcccgg attgtgcccg
     7621 accgatggcc ggccccagcg gcgtcctctc ttcggacgtt gcttgcctcg acggcagtcg
     7681 gcactaccct gttgccgatc gtagccaaca tgacgcttga gatcagcctg atcgggccca
     7741 taaccaacct ggttgctgtg cctttgggcc tcgtggccat gacggctggc ctgggcgggt
     7801 gcgtaacctg gcatgtctgg ccatggcttg ggtctgttat gaacgccggg gccgaggcgg
     7861 tactcactgt gctggcctcg ttcgtccgcg ccatggcgtc catgccgttc tcatcagtgc
     7921 ccatcaggaa gctctctgaa tgggagatag cagcctacta tgccttgctg gcctgtactt
     7981 gcgcgattgg atccgacgtt ttcaggctgc accgattccg acgggctgtg ggtcgaatcg
     8041 cgaagcgccc cgccgcagtc atgtgcagtg cggcgatagt gcttctcctc gctttcaggc
     8101 cagcgccgtt cgaagtggtc gtaatgtcag tcgggcaggg cgacgcggtt ttcatatctg
     8161 ctccagaagg cgtcaatatc ctagtcgatg caggcgccgc ttgggccggc gacaagtatg
     8221 ttgtgccata tctggagcgg cgaggagtca ggcgcctcga tcttctggtc attactcacg
     8281 agcacgccga tcatgccggc ggagccgcag cagttgcgcg atcagtcaga acggaatgtg
     8341 tcgctgtgcc cggctgctct tcgggccagg cgtgggaggc gattctgggt gggctcgaga
     8401 cggcctgcgg cggaagatcg ccccgcatgg tcggcctgag ccagggccac gtgatacgag
     8461 caggcggcgt cacgctggaa gtgctgaacc ctcctgcgaa gtcaggtgtc gcagtgggcg
     8521 gccacatggc cgacgcgaac gacgattctg tggtgctgcg cctggtttac cgggacttct
     8581 cgatgttgtt gtgcgcagat gccggggagc ggtttgaacg ccgcattctc gacggaccgc
     8641 tgcaagcaac ctccctggcc tcgcaggtta tcaagatagg tcatcatggc tcttccggcg
     8701 cctcgtcatc ccggttcctt gagactgtga gctctagctt ggcaatcgtg tcggttggcc
     8761 gtaatggata cgggcacccc agccccgctg ctgtccaccg catactcgat gccggtgccg
     8821 agatcaggca gactgataca tgcggcgccg tctccgtcag catcacttcg cgcggggcag
     8881 cgcaggccat gcgccggtgg gcgggctatg tcgcgcgtga catgcggggc gtgtgggcaa
     8941 gggctcctat gttcaggaga ctgcagatat gaggggtctg gattatcctc cgtaccgtga
     9001 ctcttccaaa atcgcaggaa cgcaacgaca aaccacgacc atgaggtttg ccggaaggag
     9061 tgagcgggta tccgtcgaat acgagtggtg cgatgctatt cagaaagggg ggtgttcccg
     9121 cctgctttgc gcaggccggg aaagcgactt gaaacgtttt gccgcaatac tgctgattgc
     9181 cgcgttcatc gtcaccactc tggctatcgg tccaacgctc gcgcagcaga agacgactat
     9241 caccttctgg cacgccatgg gtgcgcagct tggcaagacc attgacatgc tggttgagca
     9301 gttcaacaat gagaacccgg acgtgtttgt aaaggctgag tatcagggca actacggggc
     9361 actgagccaa aaactagtag gatccttagt ggcccgcaaa cctccgaccg tagcccaggt
     9421 atacggtaac tgggcggcag agtacataac ctcgaaggag ctagtccccg tagagaagtt
     9481 catcaagggg cccaacggtc tttccactct tgagatcgac gatatctggg aaggtctcag
     9541 ggcgggatgc gtcttcgacg gtgtatggta taccatgccg ttcaacaaga gtgtctatgt
     9601 gttggtctac aacgaaacgg ctttgaagga agccggaata gccaagccgc ctgcaacctg
     9661 gcaggagctg ttgacagccg ctagagcgct cacggtcaag gagggcgaca agatagttcg
     9721 ctacgggttc ggcctccgtc ctaacgtcga ctacttcgct tgcttcttct tcactgctgg
     9781 cggaacatgg ctggatgccg gcggcaaggt gaatgtgaac tcgcccgaag ggaccaaggc
     9841 tcttcagtac atggcggatc tccttagcaa gtacaaggtc tcgtactacg tgcctggcta
     9901 cctcgatgct gacttcgggg cgggcaaggt agcgatgtac ctcactacct ctcctggcct
     9961 gagctatact gaccagtccg tggccggcaa gttcgaatgg agcgcctctc ctctgccgta
    10021 catggaaccc aagttcaagg cgacacctgt tgccggcact gacctcgcca tcttcgcaaa
    10081 agccaccgag aaggagcaac aggctgcatg gaagttcgtc aagtggctgg tggagccgag
    10141 gcagactgcc aaatgggcaa tcggcaccag ctacatacct gtccgcaagt ccgccatgca
    10201 cctggagatc atgaagcagt ggtttgcgca gcatcctcgc gaggagcaga gcctgcagca
    10261 gctgcgctac atcaagtatg acccgaacat ctctgcctgg accaacataa ggggcgacat
    10321 ctcggaggcc gtggagaagg tcttcctcgg caaggccacg gccaaagagg cccttgacgc
    10381 cgcagtggtc aaggccaacg ccaggctgaa gtagcctgac gtcgggctag cgtggatcct
    10441 gcatgatcaa gaatgcccgg ggacgtcgag cttcaactcc ccgggcttca gctctgtgga
    10501 ggagacgtcc aatgcgcaaa ccattccgcg tcgcccgtac acgctttctg ccgttgctct
    10561 tcgccgtcct ggccgccaca atcgccgtcg cgctggcgct ttcggtcccg caggcggcct
    10621 cggcggaggc acttgccggc aactacaatt actatttcgg gaacctccat tcccatactt
    10681 cttattcgga cggagtgctc acgccatccg atgcgtttcg gcacgcccgc gaggccgcca
    10741 ggatggattt ccacgccgta accgatcacg ggtactacat gcaggaggcc accaacctgc
    10801 atcactggtt ccttgctcac gaagaggccg acgagttcta cgaacccggc aggtttgtta
    10861 cgcttgtggg atatgagtgg acattcactg acggtcacat gaatgggttt gctacgcccc
    10921 gggccgcttc ccgcgacacg cagcgtgacc tgaccgcgtt cctcgagttt cttgccgagt
    10981 atgacggcat tggcgtgttc aaccatccga gttacgacat tcagccgaac tggaacgact
    11041 tcgagtatcg tggactgggc gacaaacagg tttctctgat cgaggtgggc tcgggcccgt
    11101 acaggcataa cgtcaggaat gagcgtgcct acatacgagc gctggataga ggatggcacg
    11161 tgggcgccgt cagcagccag gataaccaca ggcccgattg gggaacggct gcccccactc
    11221 gaacagggct tatcgcgtcc gaactcacgc gcgagtcagt gctttccgcc atgcgcgaca
    11281 tgcgaacata cgccactgag gattccaacg tccggatgtt cttcgcgtcc ggcaacagta
    11341 ttatgggcga tgagatcgtg ctcgctccgg atgttgtcat gtcaggtaaa tccgtcgagt
    11401 ttcgtgtcta cgtggcagac cccgatgaca ctgattatct ggagctagtg cagatcgtgt
    11461 cgaacggcgg tgaagtggtg tgggaaagta ggccgggacg gcttgagccg cgcggggcgg
    11521 gtcaaggagg ccaggcgggt ggggtaggtg tggcagtcac ggcatatgac actgtcgtgt
    11581 ccctgacgcc cagatcatcc tacaactggt actatgttcg cgccgtgcaa agcgacggcg
    11641 atgtcatagt caccagtccc atctgggtca ctaccggctc cggcgtagcc gtttgcgact
    11701 ttgcctgcgt ggacacggta gttcgtgctg gagtatccgc ccggatccga gccgagatcg
    11761 tcaacaggaa tgagcaggct atacgagcag ttactgccgt gctctacgag acggcgccgt
    11821 cgggcagacg cgctgtgtcg tcgcggcgcg tcgacctagc aggatgggcc ggaacggaca
    11881 tatggttcga ctggattcct ggagcgcctg gcgagaccgg gctagagctg gaactgatca
    11941 tccccgggca cgaggcgcct cttgtcttcc cgggcgcgcc ggccttggtc cgcagggccg
    12001 acatcacgcg cgtggtgatc gatgagggcc acaacaacag gtataccggc tatatggacg
    12061 gttttgtgac gttacttgcg gcgtccgact acagcccgcg aataggggaa gagccaatag
    12121 gcccggacat gcttgccgag accgatattt tggtggtcaa tgtgccggag atcggatttg
    12181 cgctcactcc tacctctttc gagcaggacg agctggaggc gattgccgcc ttcgtagaac
    12241 aagggggatt cctgctcctt gcgggctggt ccccgagccc ggacggaacg cgcacttgcg
    12301 agcaactcaa tgtcttgctt cagctcatgc atgccggcat gggatttgcc gatgacgaat
    12361 tgcaggcgga tgggtcatcc tccgtttgcg cttactgggg gtcaagcgag gctaccggtg
    12421 gtccaatcta cgcatctgag gcgtgttcag tctcgatcga gggtagcggc gcaacggtag
    12481 cggtgttcgc gcgggctgcg gacggccgtg tctttgccgc cgggcagtct gtcgggagcg
    12541 ggcgcgtggt gtgcctgggc gcgccggtgt actcatcgct ggatctcaag agggagttca
    12601 gtaagaactc cgatttcacg ctggctctca tggtgtggct ggtcaaaggg gagtgaacag
    12661 caaattgaaa ccttcgcgta gcaggaaata cgcccgcgac acagcggcgg cctatctgta
    12721 cctggcgccg gctctgatag tactaatagt gtttcacttc ttcccggcgt tctacgccct
    12781 gtacctcagc ctgttcgaca cgaacctgtt cggaaggtgg aactgggtcg ggctgggcaa
    12841 ctatgccgaa ctcctccatg atcaggattt ccttcgttcg ctggccaaca cgggcaagta
    12901 tgtgcttggc acagttccgg tgggcatcgc ccttgcgctt gccattgcgg ggttgctcaa
    12961 tcagcgaata cgcggactgg cagcataccg aacggccttc ttcctgccgt acgtcacgcc
    13021 ggtggtggcg atttcgatcg tatggacatg gatatacaag gaagactctg ctgggatgct
    13081 caatgcggtt ctgtcctggt tcggaatcag cccacaggca tggctgctag accccaaatg
    13141 ggccatgttc gctctttgcc tgatgagcat atggaggcac ctgggctaca acgtcgtgat
    13201 ctttctggcg gggttgcaga acataccggc cgagtactac gaggcagctg agatagacgg
    13261 cgcaagcgga tgggcgatgt tttctaagat cacctggccg ctgctctcac cgactacgta
    13321 tttcatcacg ataatctcca taatcggctc atttcaggtg ttcacgcagg tatacgtgct
    13381 gtggccatcg gcgcttggcg gtcctctcgg cactacccgg gtggtcgtca agtacctgta
    13441 tgacatggcg tggggatcat accagttcgg gtatgcgtcc gccatcggct acatgctgtt
    13501 cgccataatc ttcgtgctta cgctggttca gcgcaatgta gctggtcgct acgtgcacta
    13561 tcagtaggcc ggcgggcggc ctagggggag tagaagatgt ccaaaacacg caagactgct
    13621 cacatgacct tggtgtacgt cctcctcacg gtgggcgctg tgatgatgct gatgccattc
    13681 tactacatgc tcagcacatc ggtaaaagcc cccgaggagc tctatgtgaa tagactggtc
    13741 tggttcccca gcaagatagt atggaagaac tatgccgacg catggaatga agaaccgtac
    13801 ttcccgcgct acttcttcaa ctcggcattc atcgccacag tcactacggc actgcagctt
    13861 ctgacgtcgg cgctagcagc atatgccttc tccaatatcg atttctgggg caaggaaacg
    13921 gtcttcatga ttctgctggg caccatgatg atacctagtc aggttacact ggtgcctaac
    13981 tacgccatca ttgccagact aggctggatc gacacgtaca aggccctgat agtgcccttc
    14041 acggccagcg tattcgggat attcatgatg aggcagtttt tccaaactat ccctatggag
    14101 atgtgggatg cggcccagat agacggatgc tcaaggttca ggtacttgtg ggctgtcatg
    14161 gtccccttgg ccaggccgat cttcgtgact agcggtctgt tcacgttcat cggcggctgg
    14221 aacagcttcc tgtggccgct aatcgtcacc aactcggacc atctgcgcac gatccaggtg
    14281 gggctctcac ggttcaatca ggagttcggc acccggcctg atctgctcat ggccgcatct
    14341 actctggcca tagctccgct tgtggcgctg ttcttcgtag cccagaatca gttcatccag
    14401 ggtatcgccc gaacgggctt gaagagctga cagaggagga ttggattgag gccggacgta
    14461 gctgcgacca tgaagaaagg aacgacagcc ccgatctaca tcatgtacgg cgaggatgcg
    14521 tactcccgcg ggcgcaacgt cgatgaactt gtggagcgac tcacgtccgc tcggggtggg
    14581 gccgacctga acgcattcga tgccgccgaa tcctctgtgg acgacattgc gtcggcggtg
    14641 atgaccgcgc ccatgtttgc gtctgcgcgt gtagtgatag tccgtcggtt cgaggagatg
    14701 tccacagacg accaagcccg tctcattcgg acactggctc cgggaaagcg cccggcggac
    14761 cttagggtgc cggatgagac ccaagtgatc atcgtgtcct catccaagac cgtaccacgc
    14821 aaggaagtca acgcagccgg gccccatgtg gccgcgttcg agttcaaacc ggcctatgcc
    14881 cgcgacgcca gggcttttgt gttcgagcga gccaaggagc tggaagtcag aatcgacccg
    14941 gacgccgcgt cgctcctttt ggagctggcc ggattggact acgtcgcgat atctcaggag
    15001 ctggagaagg tcgtcaccta cctagggccg agcggtcgga ggatagctgt cgatgacatc
    15061 agggcagccg tgggtaggag ttcatctcaa acagtcttcg atttctgtga tgcagtgata
    15121 gacggcaata gcaccaaggc tctgagcgcc ctggccgacc tgatccgcag cgagcgccag
    15181 gtacttgcga ttcttggcac actggccacc aagctcaggc gggtcatgga ggcccgggtt
    15241 atgcttgacg gcggcagcac acaggacgag gctgcaactg cactggtcgg cggcaacccc
    15301 aaggtgaagt ggaaggaagc ccgcacagcc gcacaggcct ctcgtctttc tcagatgcag
    15361 atagcagcca tgctctcgcg gatcgcccgg gccgacctcg acatcaagac cggcctgatg
    15421 tcggagagcc tggccatcga ggtgctcatc tcagacctgt gtgtacggca aaaactccag
    15481 taacgctggt taccggagct atgcgaaaca ctactattat gctatgcgcg atttcagctt
    15541 gacccttcgc ttcggcccgg gctaatcaac ctgtgctgcc ttattcgccc tgcgtgcgag
    15601 ccgcgacttg cgcctcgcgg cctggttctt gtgaatcacg cccttcttcg ctgcgcgatc
    15661 caccgtgctg aaagcgcgat tgagttcgtc tacagagcct tggggacccg cctggccgag
    15721 agcctcattg aacctgcgga tggcagtcct gacggaggat ttgatggcag tattccttgc
    15781 cgtccgtatc tttatggtct tgacacgctt ctttgccgac ttgatgtttg gcaccttgcg
    15841 tcacctcctc cgccgctaga ttgcacgact atgattctac catgcacgaa gtgccattgc
    15901 aagcgtgacc aacgcgcgat caatgccgct agcgaggtgt tgcgcgctcg ccggttgggc
    15961 aagactactc ctgatagtgt gccgacaacg cgccggcacg ccattgcagg gaggggttgc
    16021 tcttggattt tgaccgcgct ttcggctcga aatggcgccc gtgctcaagg ctagagcaca
    16081 tgtctcgaga agagtcatcg ccgggaggtg gctcgttgcg aaccgacttg gctgtcgagg
    16141 cacatgaagt tctcaggcag ggcatggggc ctgagatccc aggagtaagc accagaacag
    16201 atgagactga cttcggctcc ataacccggg tggctatcac tactccccag gcggcgaggg
    16261 ccctcggaaa agcaatgggc aactacataa ccattgatgc tccagatctg cccctgcgtg
    16321 atcgcgaact cgaaagcgag gtctcgcagg cgctcgcacg tgaactggtc ggcctcatac
    16381 ggaccagtct gaacaacgtg gacttctggg cggtgccgga ttttaccacc ctcatctgcg
    16441 ggttgggaaa ctggaacgcc acgcctgatg ccatcggccc gcttgtagcc ggcaaggtca
    16501 tggtcacccg ccatgtgtat tccatgaccc c
//
LOCUS       DAOTAC010000013        15930 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_3055_length_15930_cov_4.534299, whole genome shotgun sequence.
ACCESSION   DAOTAC010000013 DAOTAC010000000
VERSION     DAOTAC010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 15930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15930
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3055_length_15930_cov_4.534299"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1151
                     /locus_tag="PLM74_01155"
     CDS             <1..1151
                     /locus_tag="PLM74_01155"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HON41724.1"
                     /translation="MTAPVVRQIVKDFEAANPDIKVNFQAIPWGEDPHTKFQAAVVGG
                     TIADVFTVGDPFEHVLAEAGALEPLDKYLTADMKADFYPQYLDRCTANGQLVALPWFG
                     DVRPMLYRKDLFQAAGVPEPAGSWTWDEFAAYARKLTRDLNGDGIIDQYGFGTSGRYV
                     SQFQPFLRQNGVDFVDEAKNIATANDPAAVEAVQFYVDLIRKHKVTPPGITTIALQDI
                     QKMFAEGKVAMFFDASDTATRLSKEPSLAGKLGVALLPHKKAHGAFGGADVVVMSKQS
                     KNKEAAWRFISYFLSREGMLTYCKGVGFSPVRKSAASDPFFTDDVIKKTYAKQMEVGG
                     FFYFKHPRASEMTPLIRAEVQAAMEGTKTVPQAMNDLQKQLEGVLRGR"
     gene            1271..2173
                     /locus_tag="PLM74_01160"
     CDS             1271..2173
                     /locus_tag="PLM74_01160"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HON41725.1"
                     /translation="MKQRPAWRRMGKTLESYTMLAPAYMIFLVFIFIPVAWAFYLSLF
                     DYSIMSLRAPRFAGMNNFRRLVSDRLFWISLKNTARYALGTVPWRMILGLMLALVLDQ
                     TKLVGRKLLRTVYFLPVVTSMVAAAIVWSLVFSASNSGLANQLLAVFGMAPRGWLADS
                     RLAMFSVCVLSVWKDLGYVMTIFTAGLQGVPLELYEAGAIDGASTWQRIRFITIPLLR
                     PTTFFILVTEILGSFQVFTQTYVMTQGGPGYVTTTLINLLHTKGFVQYDMGYASALAV
                     AMFIGLLIMSWVLRKVFRAEEIVY"
     gene            2196..3092
                     /locus_tag="PLM74_01165"
     CDS             2196..3092
                     /locus_tag="PLM74_01165"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HON41726.1"
                     /translation="MTVMPDATVGQKSKRKTEVSCGRITAGVVQYAALIIGAFVMLAP
                     VLWMISTSLTADSVIVSYKLIPTRVTLENYVRAWTFPSNFDESVTLGTFFLNSVLVSA
                     LITGPALIIDSLAGYVLARRKVPGSNLLFYMALATMMIPFYVIAVPLFLTVRKLRWLD
                     TYQGMVVPFLASGFGIFMFKQFFQTVPQDLEDAALVDGCSAWRTYWSIMLPLAKPVVG
                     TMAIFKVMWSWNQFFWPLLIINNIRLKTLPLALTMFRGLNVTQWGTLCAGMTVATIPV
                     VVVYLSMQDMFQKGITMGAVKG"
     gene            3089..4915
                     /locus_tag="PLM74_01170"
     CDS             3089..4915
                     /locus_tag="PLM74_01170"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HON41727.1"
                     /translation="MMREAANGPIGRLLGEFTPNERSVDRVLAGLSLRRKIGQMMVVG
                     FPERFDGFEGRLPDGILSLLADPGVGGVGLFARNAKDPAEMLALTSSLQEAAMAAGAP
                     VPLFIMADQEGGIVMQVTAGATVFPGNMALAATGEPAIAYEATRIIGLEALAMGLNAV
                     TAPDVDVNADADNPIIGVRAYSDDPALVGALGEQAVRGFGSVGLFCTAKHFPGHGRTA
                     VDSHLGMPRLDGSAKDLESVDLPPFAAAIAGGATGIMTAHIQSEALDPSGACATLSRP
                     ILTGLLRERLRFDGVIVTDCLEMGAIQQGVGTARAAVEAVVAGADMVLIAHTIALQRQ
                     AADMLEQAVRSGEISEERIDLSCRRTIRTKLALEAACRRMWDADGRPPLEIVGCAEHL
                     LAEQRIARQSVTLIRNEDGVLPLAANEVRRIAVVYPATMPRLRAEDIDDDQTVLGREI
                     AARGIEVVEIGVGLTPTTGEVAAVCDAVVGAQVDAVIVATSCKTTEQEKAQGALVQAL
                     LEAVRGADARLTETPETAGSAEFGGRETPVIACAIRNPYDIRSYPDVRTYLATYGYRP
                     CSIRAAVEAVFGEIVPRGRLPVEIPGLYPRGHGLAGFASDYS"
     gene            5215..6384
                     /locus_tag="PLM74_01175"
     CDS             5215..6384
                     /locus_tag="PLM74_01175"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HON41728.1"
                     /translation="MPRSARLVCCCLIACMAFALYVSHPAMADVAQQKRLAVWMWGST
                     VRSQGAEAIAKKLADERVTDVILLVRGTSGKAEYASAIAPPAEAGKDALGDIIAACRP
                     LGIRVHAWFVFNQDKAYTDAHPEDRIWHHGKANTEFKPYEIDDGKVCPASAAHLEYTN
                     SLIREVMEKYSVDGIHLDYIRYGHMVYCFCPAHIEKATKLGINVDKVREAMFQTLYAS
                     PANPNHLFEAYAAGEPDVVAWVNMRQAEVREAAASMRAAAKSINPNVVFSASLMPEGA
                     IDESKPFAMCHYGQSYDDAGKLYDFVCPMAYHIDYSKPAEWVGDITRGTVQAVGDVPI
                     YIGLQAYPDKCTYDELKAAALAGLDAGAAGVALFRFGTMTGPRWAAIKDVMEGTK"
     gene            6487..7494
                     /locus_tag="PLM74_01180"
     CDS             6487..7494
                     /locus_tag="PLM74_01180"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HON41729.1"
                     /translation="MDLVIGVDGGGTKTACAVADMEGNLLGLGLAGPSNYLRLAGGAR
                     EAAQAVRDGAEAAMRDAGLYMSASSCRIRVAQLAVGGVGIDGVNVAMSEAARAVLDAE
                     NVCVENDAAAGLAAAVGELYGVVVVSGTGSIAMAVDRAGRRARSGGWGYRVGDEGSGQ
                     DIGRLGLAAATRAYDGRGPATSLVERALARHGIDTLDGLRGVLYRQPVNNRDIADFCL
                     DVVAAAQEGDAVALGILEHAGRELALAVCAAARALGMDAGEFPVAPCGSVFKAGDVIL
                     DPFKGELARLVPGARVVHAPYPPVVGAVILALLRAGVACDEAIRRRLAQSWKEVEKRH
                     E"
     gene            7487..8737
                     /locus_tag="PLM74_01185"
     CDS             7487..8737
                     /locus_tag="PLM74_01185"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HON41730.1"
                     /translation="MNEFLRAASGMRDDIERFFEQLHAMPEGPFAETKTAGFIAERLA
                     DVGYRVRAGLAGTGVVADLERVGEASLDQVVCPIIPTSADPAPPENPIVSGDASAPAL
                     LALRADMDAMLHSLDGKEVYVHSCGHDAHSTMVSAAGVILARKCPKARGRLRLVFQPA
                     EETGLGAERMIEAGAVEGVDAMFGIHLRPAAECRLGQATPCLVHAGGRTLQYVVKGQP
                     SHTGRPHLGVNAADAVALAAIAVNSVKPDPLACATVNVVGLGAGGPGGKPSGVIPEQG
                     ELTVNIRAESDEMADELRVRVDSAVRAAAGSIGAGVWLEREKKMPAPCYDPDMIDIAR
                     SAIVAVLGEAGVRERITTPGSEDFHWYARRKAGLRAAYIGLGADFVPGLHHPDATFDR
                     QALVYGAAILAQAAACYFDRAVQA"
     gene            8786..9913
                     /locus_tag="PLM74_01190"
     CDS             8786..9913
                     /locus_tag="PLM74_01190"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HON41731.1"
                     /translation="MAEPCGVERAGARADAQVDRSDLDGPRAPRPDELDAVLDLVNNT
                     FSPVGRTMGLEFPQLLSDANLARLRVFVDPKTGLPVAHAGYVRCELAVEGGRIPVAGL
                     GSVCTDEAYRGMGLASELVESIMRQAVDEGLVMMLISGGSNVYRRLGAVDTGDFLRVE
                     ANREQVSSLRNAAVDWPLHSAAAIRLAVEDDIPALASIYRREPVRYIRSVSDFRELAW
                     QKPDVKRLGSMERTWVGGLVRDSLAYIIVRIRTADDGAIRLHVAEFAGARCCVLQMIA
                     HACAEYDPDLLTIAVPVSDLEMGALLHQAGLTLHRVGLLGHTVMSLDDSALLKCVYPI
                     MSERFGADWTAPNHPPAGPELIRWLFSDLGIPLPVPGLNYV"
     gene            10333..11061
                     /locus_tag="PLM74_01195"
     CDS             10333..11061
                     /locus_tag="PLM74_01195"
                     /inference="COORDINATES: protein motif:HMM:NF002805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HON41732.1"
                     /translation="MFDYFDLVRELLDKVQRSEQESMERAATTIADTVARGRVLHYFA
                     AGHSHILGEELFYRAGGLAPVNAIIEPSLMVSNGASKSSKLECLHGLAKVILEESGVE
                     RGDVLMVASTSGVNAVPVEMATEAAELGARVIGITSIGASKATPLRNGSGKRLFELAD
                     IVIDTHVPYGDAAIQVEGVTQRIAPLSTVIGVAIVNALVVRTVKILVEQGIEPPVFAS
                     ANIPGGQEFNVALLKRYKDQIRAF"
     gene            11127..12338
                     /locus_tag="PLM74_01200"
     CDS             11127..12338
                     /locus_tag="PLM74_01200"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HON41733.1"
                     /translation="MRTTPANSVDSETRETRETWAIRTGQGVDARGQVQGPLVAAVDG
                     GGTKTLFLLATADGTVIGIGRGGPVNALFASADEAIRSVQQAAAGALREAGMKCADVA
                     VLYASAPGASRDIVSAGIDGLVSPARLETAGDEMAVLTGALVDEPGVVVLAGTGSFAV
                     GRGIRRDCDSQAGDTGRHRVVTRYRIATRGGWGPLLGDEGSAYAIGLAALRAAALAFD
                     NRGEHTCLCDMALEHFDAPHAWSLATMNMSREQIASFAVRVSQASAAGDDVASAILAQ
                     AGRDLGRLGVSVLRELAERSATGEPGEPVEPVEPVEPIEPVESGNAGHGRRILAVLTG
                     GVSRAALPLIAAFRDEILSFSPDAQIVPPQFSPAGGALLVALEIAELREPGMVSAGLR
                     ASAEACSELRA"
     gene            12421..13167
                     /locus_tag="PLM74_01205"
     CDS             12421..13167
                     /locus_tag="PLM74_01205"
                     /inference="COORDINATES: protein motif:HMM:NF002805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase domain-containing protein"
                     /protein_id="HON41734.1"
                     /translation="MLGKGAAYLDEIRSLLDKIQTTQAENIAEAADMIVDAVSHGGAV
                     HVMDTGHMLMHELFGRTGGLMMLRPVNITMDVQNPGPARAGKVKPKVYLDQIPGLPQF
                     VLDRSDIFAGDVLIIGSVSGKNTLPVGLALLAREQGVKVIALTSVAYSAALQGEHPSG
                     KRLFEAADVVLDNCGIVGDAALDIEGIDAKVGPTSGIAAAAIMWALEMEIMERMARRG
                     MKPSVWLSNHMPGAGEFNRRSREECDKRGY"
     gene            13338..14273
                     /locus_tag="PLM74_01210"
     CDS             13338..14273
                     /locus_tag="PLM74_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HON41735.1"
                     /translation="MATSLSDSALSPTLPSTGSRRRRRRETLTAWSFILPAVIGLGIF
                     VYGALVYSLYISFTSWDLLTPAKWVGLANYARLFRDESFHQCMYNTLYFVVAIVPAGI
                     ALAMAMAVALNRPLKGVAFFRAAYYMPSITSTVAIGLVWLWIFNPDQGILNTILRALG
                     ATNPPRWLESMRWAKLGLSIMRLWQVSGYYMIMYLAGLQNIPKELYEAAETDGATPWQ
                     KTRHITVPLLSNTTFFATVMLLIEVFNLFEAVYVMTEGRPGGSTNTVLYYIYTEAFQS
                     YRMGYASAIAWVLFVILFVATLVQFMVRRRNERVG"
     gene            14298..15140
                     /locus_tag="PLM74_01215"
     CDS             14298..15140
                     /locus_tag="PLM74_01215"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HON41736.1"
                     /translation="MKVKIRPDQVVLYVVLVLLAAIMIYPFVWSVTASFKTNRQIFSG
                     NPLNLIPHPATLDNYRQAFALMPFGRFLLNSAMLAIVVPAASILIGSMAAYAFARMDF
                     TGRDFIFLALLGVMMMPGHITLIPRFALVRKLGWINKYTALIIPAIFSGQLVFNIFLM
                     RQHFLSVPKELEEAALIDGCSRWGVWWKVVMPNSRPAIATLAIIGFKSEWNSFLWPNI
                     VINDYMKMPIQVGLSCLQSNMRTGWDVLMAGTTIAIIPLIIIFLCFQRFFISGVMTSG
                     MGGR"
     gene            15254..>15930
                     /locus_tag="PLM74_01220"
     CDS             15254..>15930
                     /locus_tag="PLM74_01220"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HON41737.1"
                     /translation="MRRISGRVGLICLAALLVLSMSVAASPKAARTKVQVISWWDFTN
                     SRPLIELKAKFEELNPDLELDYIRIGTGYADKMLVMIAGGADLPDVMMIAMDKVPIFA
                     DKGAIMKLDKYIESDYKAEMAKLYPVVADALTVKGSYYAMPRDITSKVMFFNKQLFAE
                     AGVAIPGPDWTWDDFKALAKKMTRDTNKDGVTDQWGFYFPKYTDGWTDWLMQNEGGLV
                     TKEGQSLL"
BASE COUNT         3108 a         4337 c         5219 g         3266 t
ORIGIN      
        1 cgatgacggc gccggtcgtg cggcagattg taaaggactt cgaggcagct aaccccgaca
       61 tcaaggtgaa ctttcaggct atcccgtggg gagaagatcc gcataccaag tttcaggcag
      121 cagtggtcgg gggcactatc gccgacgtgt tcactgtggg cgatccgttc gagcatgtgc
      181 tggccgaagc cggggctctg gagccgttgg acaagtatct gaccgcagac atgaaggcag
      241 acttctaccc gcagtatctc gacaggtgca ctgccaatgg tcagctggtg gcgctgccgt
      301 ggttcggcga tgtccgcccc atgctatacc gcaaggatct tttccaggcc gcaggcgtgc
      361 ccgagcctgc cggttcgtgg acatgggatg agtttgcagc atacgccagg aagctcactc
      421 gcgacctgaa cggggacggt atcatagacc aatatggctt cggcacgtcc ggcaggtacg
      481 tatcacagtt ccaaccattc ctgcgccaga acggagtgga cttcgtagac gaggccaaga
      541 acatcgcgac ggccaacgat cctgcggcag ttgaagcagt ccagttctac gtggacctga
      601 tccgcaagca taaggtgact cctcccggga ttaccaccat cgcgctgcag gacattcaga
      661 agatgttcgc cgagggcaag gtggccatgt tcttcgacgc atccgacact gcgacaaggc
      721 tgtccaaaga gccgtcgctc gccggcaagc tgggcgtggc cctactgcct cataaaaagg
      781 cgcatggcgc gttcggcggc gctgacgtcg tggttatgtc gaagcagtcc aagaacaagg
      841 aagcagcctg gaggttcatc tcctacttcc tgtcccgtga aggaatgctt acttactgca
      901 aaggtgtggg cttctcgccg gtgaggaagt ctgctgcatc cgacccgttc ttcactgacg
      961 atgttatcaa gaagacctac gccaagcaga tggaagtggg cgggttcttc tacttcaagc
     1021 atcccagggc atcggagatg actcctctta tccgtgctga agtgcaggcg gccatggaag
     1081 ggacgaagac ggtcccgcag gcgatgaatg atcttcagaa gcaactcgaa ggcgtgctta
     1141 ggggcagata gtcatgggct tatagcaaca acggggagta acccccgcga gctcggttcc
     1201 cagcagcttg actatggtac ggggctggcg ggggtctcca tctactcttt gccgaacggg
     1261 gggataggcc gtgaaacaac ggcccgcttg gcgccgaatg ggcaagacct tggagtccta
     1321 caccatgctt gccccggcct atatgatatt tctcgtcttc atattcatcc ctgtcgcgtg
     1381 ggccttctat ctcagcctat tcgattacag catcatgtcg ctcagggctc cgcgattcgc
     1441 aggtatgaac aatttcagga gacttgtgag tgatcgcctg ttttggattt ccctgaagaa
     1501 cacagcccgg tacgcactgg ggactgttcc gtggcgcatg attctgggac tgatgctggc
     1561 tctggtcctg gatcaaacga agttggtcgg gaggaagctg cttaggaccg tatactttct
     1621 tcctgttgtc acgtcaatgg tggctgctgc catagtctgg tccctggttt tcagcgcgag
     1681 caacagcggc ctagcaaacc agttgctcgc ggtgttcggc atggcgccta gaggatggct
     1741 ggctgattca cgtctggcga tgttctccgt atgtgtgtta agtgtgtgga aggatttggg
     1801 ttatgtgatg accatattca ctgcgggtct gcagggcgtg ccgttggagc tgtatgaagc
     1861 aggggcaatc gatggcgcct ccacctggca gaggattagg ttcataacga ttccgctcct
     1921 gaggcccact acgtttttca tactcgtcac ggagattctt ggctcgttcc aggtgttcac
     1981 gcagacatat gtcatgaccc agggagggcc aggatacgtc actacaacct tgataaacct
     2041 tctacatacc aaagggtttg ttcaatatga catgggatac gcaagcgccc tagctgtggc
     2101 gatgttcatc ggcttgctca tcatgagttg ggttctccgg aaggtgttcc gagcagagga
     2161 aatcgtgtat tagtcgctga ctgggggtag gtggcatgac agtgatgcct gatgcgactg
     2221 tcgggcagaa gagcaagagg aagacagagg tttcgtgcgg ccggattacc gcaggggtcg
     2281 tgcaatacgc tgcgctcatc atcggagcgt ttgtaatgct ggcaccggtg ttgtggatga
     2341 tatccacatc cctcacggcc gattcggtaa ttgtctccta caagctcata ccaacccgcg
     2401 tcacgttaga aaactacgta cgggcctgga cgtttcccag taacttcgac gaatcggtga
     2461 ctctggggac gttcttcctg aacagcgtac ttgtgagtgc gctgatcaca ggccccgcgc
     2521 taatcataga ctcactggcg ggatacgtcc tagcacggcg caaagtgccc ggaagcaatc
     2581 ttctcttcta catggctttg gctactatga tgattccgtt ctatgtaatc gctgtcccgc
     2641 tgtttcttac cgtaaggaag ctacgctggc tggatactta tcagggcatg gttgtgccct
     2701 tcctcgcatc tggcttcggg attttcatgt tcaaacagtt ctttcagacc gtcccacaag
     2761 atcttgaaga cgcagcgctg gttgacggct gttcggcgtg gcgtacctac tggtcgatca
     2821 tgcttccgct tgcgaaaccg gtggttggga caatggccat attcaaggta atgtggtcct
     2881 ggaaccagtt cttctggcca ctgctgatta tcaacaacat ccgcctcaag actttgccgc
     2941 tggctcttac gatgttcaga gggctcaacg tgacccagtg gggtacgctg tgcgcaggga
     3001 tgaccgtagc taccattcct gtagttgtcg tgtacctgtc tatgcaggac atgttccaga
     3061 agggcatcac tatgggggct gtgaagggat gatgagagag gctgcgaatg ggccgatagg
     3121 ccggctacta ggcgagttta ctccgaacga acggtccgta gatcgggttc tggccgggct
     3181 cagcctgcgc cggaagatcg gccaaatgat ggtggtgggt tttccggagc gcttcgatgg
     3241 cttcgaaggc aggcttccgg acgggatact ttcattgctt gccgacccgg gggtcggcgg
     3301 tgtggggctg ttcgcccgaa acgcgaaaga tccagcggaa atgctggcgc tcacgagcag
     3361 tctgcaagaa gcggcgatgg cggccggagc gccggttccg ctatttatca tggccgacca
     3421 ggaaggcggc atagtcatgc aggtcactgc gggggcaacg gttttccccg gcaacatggc
     3481 ccttgccgcc acaggcgagc cggcgattgc atacgaggcg actcgtatca tcggccttga
     3541 ggcgctggcc atggggctca acgccgtgac tgcgccggac gtggacgtga acgccgatgc
     3601 cgacaacccc ataatcggcg tgcgtgccta cagcgacgac cccgcgttgg ttggagcgtt
     3661 aggggagcag gccgtgcgcg ggttcgggtc ggtgggcttg ttctgcacgg caaagcactt
     3721 tcccggacat ggcaggacgg cagtcgattc gcatctgggg atgccgcggc ttgacggcag
     3781 cgcgaaggat ctggagtcgg tggacctgcc gccgtttgca gcggctatcg cgggcggggc
     3841 gaccgggata atgactgcgc acatccagag tgaggcgttg gatccgtccg gggcatgcgc
     3901 taccttgtcc cgtcccatac tcacggggct tctccgtgaa cggctgcgat ttgacggagt
     3961 aatcgtgacg gactgcctgg agatgggagc catacagcag ggtgtaggga cggcgcgggc
     4021 ggcggtggag gcggtggttg ccggggcgga catggttttg attgctcata ccattgccct
     4081 gcagaggcag gccgcggaca tgctggaaca ggcagtgcgg tcaggtgaga tctccgagga
     4141 acgcattgac ctgtcctgca gacggacaat ccgcacgaag ctggcgctcg aggcggcatg
     4201 ccgcaggatg tgggacgcgg acggcaggcc cccgctggag atcgtcggat gtgcggagca
     4261 cctccttgcc gagcagagga ttgcccgcca gtcggtgaca ctgatccgca atgaagacgg
     4321 agttttgccg ctcgccgcga acgaagtgcg gcggatcgca gtcgtgtacc ccgccactat
     4381 gccgaggctg cgggctgagg atattgacga tgaccagaca gtgctgggca gggagattgc
     4441 ggcgagaggg attgaagttg ttgagatcgg cgtggggctg accccaacaa ccggcgaggt
     4501 tgctgcggtg tgcgatgcgg tggtcggcgc gcaggtggat gccgtgatag tggcgacatc
     4561 gtgcaagacg accgaacagg agaaggctca gggcgctctg gtgcaggcgc tgcttgaggc
     4621 ggtgcggggg gcggatgcca ggttgacgga gacccccgaa actgctgggt ccgccgagtt
     4681 cggcggacgc gaaacacctg tgatagcgtg cgcgattcgc aatccttatg acatccggtc
     4741 ttatccggat gtgcggacct acctggcaac ctacggatat cggccatgct cgatcagggc
     4801 ggctgtagag gctgtattcg gcgagatagt cccgcgagga aggctgcctg tggagattcc
     4861 cgggctatat ccgcgcgggc atgggttggc cggtttcgcg agcgactata gctaaccaga
     4921 cccaaagtga gacgaactcg tctcgatggc gacgtgcggt ttgggttgca gacgaggcag
     4981 agcctgcagt aggaaaagcg tagcggcgat ctccaggacg gatggtagac cgcggtgtcc
     5041 tcttttgttg ccatggatct ccagttctga ggccaatggg acgccgaaca agcagcagcg
     5101 tacggcgcgc atgcatcggc tgctatccag cgtaacgggc ccgcttccgc aacggttgcc
     5161 tgtaggatag atcggatcac acaaaacaga aagtgaatag gaggttcgga aatcatgccg
     5221 agaagcgcac gattggtttg ttgttgcctg attgcgtgta tggcgtttgc cctgtacgtg
     5281 tcccacccgg cgatggccga tgttgctcag cagaagaggc tggcggtatg gatgtgggga
     5341 agcactgtga ggtcgcaggg cgccgaggct atcgcaaaga agttggctga tgagagagta
     5401 accgacgtga ttctgctggt gcgcggtacg tcgggcaagg ccgagtatgc aagcgcaatt
     5461 gctcctcccg ccgaggcagg gaaggacgca ctgggcgaca tcatcgcggc gtgtcgacct
     5521 ctggggatcc gagtgcatgc atggttcgtg ttcaaccagg ataaggccta caccgacgct
     5581 caccctgaag acaggatatg gcaccacggg aaggccaaca ccgagttcaa gccttacgaa
     5641 atcgacgatg gcaaggtgtg cccggcgtcg gctgcccacc tcgagtacac gaattccttg
     5701 atccgagagg tcatggagaa gtattccgtg gacggaattc acctggacta tatccgctac
     5761 gggcacatgg tctactgctt ctgccctgcc cacatcgaga aggccaccaa gctcgggatc
     5821 aacgtggaca aggtgcgcga ggccatgttc cagaccctct atgccagccc ggctaacccc
     5881 aaccatctct tcgaggccta cgcagccggc gagccggacg tcgtggcctg ggtcaacatg
     5941 cgccaggcgg aggtacgcga agcggccgcg agcatgcgcg ctgccgccaa gtcgatcaac
     6001 cccaacgtcg tcttctctgc aagcctgatg ccggagggcg cgattgacga gtcgaagcct
     6061 ttcgccatgt gccactacgg acagagttac gatgatgcgg ggaaactgta tgacttcgtg
     6121 tgccccatgg cgtaccacat cgactactcc aaaccggcgg agtgggtcgg ggatatcacg
     6181 cgcggcacgg ttcaggcggt gggcgacgtg ccaatataca tcgggttgca ggcatacccc
     6241 gataagtgca cctatgacga gctcaaggcc gccgctctgg ccggcctaga tgctggcgct
     6301 gctggcgtag ccttgttcag gttcgggacg atgaccggac cgcggtgggc agcgatcaag
     6361 gatgtcatgg agggcaccaa ataggaagac ggctgtaggc aggcacggag gaaatatcgg
     6421 gatttcggtg aacaacaggt agttgggtgg acggctggag ccgtgggatg actggaggac
     6481 ggcggaatgg atcttgtcat aggcgtagac ggcggcggca ccaagaccgc gtgcgctgta
     6541 gcggatatgg aagggaatct cctgggcctg gggttagccg ggccatccaa ctacctgagg
     6601 ctagccggcg gcgctcgcga ggccgcgcag gcggtgcgcg atggggcgga agcggccatg
     6661 cgcgatgcag gcctttacat gtcggcgtct agctgcagaa tacgcgtggc gcagctggcg
     6721 gtgggcgggg tcggcattga tggggtgaat gtcgccatga gtgaagccgc ccgcgccgtg
     6781 ctggacgcgg aaaatgtctg tgttgagaat gatgccgccg ccgggctggc ggcggctgtg
     6841 ggtgagcttt acggtgtggt ggtcgtgagc ggcacaggca gcattgccat ggcggtggac
     6901 cgggccggta ggcgcgctcg gtcgggcgga tgggggtacc gcgtgggcga tgagggaagc
     6961 gggcaggaca taggccgcct aggcttggcc gctgccaccc gagcctatga tggacggggc
     7021 ccggcaacca gcctcgtgga gcgggcgctg gcccgccacg gaattgacac tctcgacggc
     7081 ctaaggggcg tgctatacag gcagcctgtg aacaacaggg atattgccga cttctgcctt
     7141 gatgtggtgg cggctgcgca ggagggcgat gcagttgcac tgggaattct ggagcacgct
     7201 gggcgtgagc tggcgctagc ggtgtgcgcg gccgcgaggg cattgggcat ggacgccggc
     7261 gagttcccgg tggcgccgtg cgggagtgtg ttcaaggcag gcgatgtgat tctagacccg
     7321 ttcaagggcg aactcgcgag actcgtgccc ggggcgcggg tagtgcacgc gccatatccc
     7381 ccggtggtgg gcgcggttat cctggcactg ctacgggcgg gcgtggcttg cgacgaagct
     7441 atccggcgtc gacttgccca atcatggaag gaagtcgaaa agcggcatga atgaattcct
     7501 acgcgcggca agcggtatgc gggacgatat tgagcggttt tttgagcaac ttcacgccat
     7561 gcccgaaggc ccatttgccg aaaccaagac ggccggcttt attgcggagc gcttggcaga
     7621 tgtgggatac cgggtccgcg ccggtctggc cggaacgggg gtcgtggcgg acctcgagcg
     7681 cgtgggcgag gccagcttgg accaggtggt ttgtcccatc atccctacgt ctgcagaccc
     7741 tgcgcccccg gagaacccga tagtctcagg agacgcttca gccccagcac tcctcgcgct
     7801 gcgcgccgac atggacgcca tgctccacag tctggacggg aaagaggtct atgtccactc
     7861 atgcgggcat gatgcgcact caaccatggt gtcggccgcc ggggtcattc tggcgcgcaa
     7921 gtgcccaaag gcgcgcgggc gcctcagact ggtctttcag cctgctgaag agaccgggct
     7981 tggggccgag aggatgatag aggccggggc ggtcgagggc gtggatgcca tgttcgggat
     8041 ccatcttcgc ccggcggccg aatgccgttt ggggcaggcg accccctgtc ttgtgcacgc
     8101 gggcggcaga acgctacaat acgtggtgaa gggacaaccg tctcacacag gaaggcccca
     8161 tctcggagtg aatgcagccg atgcggttgc cctggctgca atagcagtca acagcgtgaa
     8221 acccgatcca ctggcgtgtg ccacagtcaa tgtagtcggc cttggggcgg gcgggccggg
     8281 cgggaagccg agcggagtca tacccgagca gggcgagttg actgtcaata tccgggccga
     8341 gtcggacgag atggcagacg agctccgcgt gcgcgtcgac tccgcggtac gggccgcggc
     8401 gggctcgatt ggcgccggtg tgtggctcga acgcgagaaa aagatgccgg ccccctgcta
     8461 cgatccggac atgattgaca ttgcccggtc ggcgatagtc gcggttttgg gcgaggcagg
     8521 ggttagggag aggattacta ctcccgggtc ggaggatttt cactggtacg cgcggcgaaa
     8581 ggcagggctg cgggcggctt acatcggcct gggagcggat ttcgtgcccg ggcttcatca
     8641 tccggatgcc acattcgacc ggcaggctct ggtgtacggc gccgcgatac ttgcccaggc
     8701 tgcggcatgt tacttcgacc gcgcagtgca ggcatgaggt gttacgcgga tggcgaacca
     8761 caccaacaca aagggagtgg ggacgatggc ggagccctgt ggagtggaac gtgcgggagc
     8821 ccgggctgac gcccaggttg accgctctga tctggacggt ccgcgcgcgc cgcgccccga
     8881 tgagcttgac gcggtgctcg acctcgtcaa caacacattt agccctgtgg gccgcacaat
     8941 ggggctggag tttcctcagc ttctcagcga tgcgaacctg gcaaggctca gggtgtttgt
     9001 ggacccgaag accgggctgc ctgtggcgca cgctgggtat gtccgctgcg aactcgccgt
     9061 tgaaggtggt cggatccccg tggccggctt ggggtcggtc tgtaccgatg aagcttatag
     9121 gggcatgggg cttgcatccg agctggttga aagcatcatg cggcaggccg tggacgaagg
     9181 gctggtcatg atgttgatat cggggggctc caacgtgtac aggcgcttgg gcgccgtcga
     9241 cactggagac ttcctgaggg tagaagcgaa ccgagagcag gtatcgagcc tgaggaacgc
     9301 tgcggtcgac tggccgctgc attcggctgc agcaatacgg ctagctgtgg aagacgatat
     9361 ccccgcgctg gcgtccatct ataggcgtga accagtgcgc tacatccgta gcgtatcgga
     9421 ctttcgcgag ctggcctggc agaagcctga cgtgaagcgt ctgggatcga tggagcgcac
     9481 gtgggtgggc ggattggtcc gcgacagttt ggcctatatc atagtacgga tcaggacggc
     9541 agacgacggc gcaatcaggc tacatgtggc cgaattcgcc ggggctaggt gttgtgtgct
     9601 gcagatgatt gcgcatgcgt gcgccgaata tgacccggac ttgcttacca ttgccgtgcc
     9661 cgtgtccgac ctcgagatgg gggccctgct gcatcaggcg ggtttgaccc tgcatcgtgt
     9721 gggccttctg ggccatactg tgatgtctct agatgatagt gccctactga agtgcgttta
     9781 ccctatcatg agcgaacggt tcggcgcgga ttggacggcg cccaaccacc ctccagccgg
     9841 gcccgaactg atccgttggc tattctccga ccttggaatc ccacttcccg tgcccggtct
     9901 gaactacgtg tagcggtcac gtcttgtcaa gtcgcatcag gggctccttg cctggaatcg
     9961 gctcctgtac cctacgggac accttttcca gccatcctgt gtcggattac ccaggccccc
    10021 cggaaatcct gtcccacagg gcacaggtcc gcagagcccg tcggtgaccg tcgcccccgg
    10081 cgaaaccctc cgccacaaga accccgactt caaccccaca acgaccgtga tcccgcgtcc
    10141 ggcgatccag ccgaacttgc ccgatcgcgc attcgcctga tttggggtac gtgatttgtg
    10201 gtccgcggtc ttgatattca ttataaacaa tatataatgg agacagggca tccgatgatc
    10261 gggtccaagg cgggcatgga aaccggcaga tagcgacggc gccggcggcg tcatcctggg
    10321 aggtagaata aggtgttcga ctatttcgac ttggttcgcg agcttctgga taaggttcag
    10381 cggtccgagc aggagagtat ggagcgagca gccacgacga tcgcggacac cgttgctcgc
    10441 gggcgtgtgc tccactactt cgcagccggt cactcacaca tactgggtga ggagctgttc
    10501 tacagagccg gcgggcttgc tccagttaac gcgattatcg aaccctccct gatggtctcg
    10561 aacggcgcat cgaaaagctc caagctggag tgcctgcacg gcctggccaa ggtgatactt
    10621 gaggagtcag gcgttgagcg cggcgatgtg cttatggtgg cttctacatc cggggtaaac
    10681 gctgtgccgg tggagatggc taccgaggcg gccgaactgg gcgcccgcgt gatcggcatc
    10741 acatcaattg gagcttcgaa ggcgacacct ctacgaaacg gctccggtaa gcgcctgttt
    10801 gagctggccg acattgtaat cgatacgcac gttccgtacg gcgacgcagc tattcaagtt
    10861 gagggggtca cgcagagaat cgccccattg tcgacagtaa tcggagtagc tattgtcaat
    10921 gctcttgtgg ttcgaactgt aaagatatta gttgaacaag gcatagagcc gccggtattt
    10981 gccagcgcga acatccccgg cgggcaggag ttcaacgttg cgctgctgaa acggtacaaa
    11041 gaccagatca gggcgttttg agtggccatg agccatcaca ggatactacc acgaagcaca
    11101 acggactaga gcggaggtag acgcccttga ggaccactcc cgcgaactcg gtggatagcg
    11161 aaacccgcga aacccgtgaa acctgggcaa tccggacagg tcagggggtc gacgcacgtg
    11221 ggcaagttca gggtccgcta gttgcggccg tcgatggcgg gggcaccaag acgctcttct
    11281 tgctggccac cgcagatggc actgtgatcg gcatcgggcg gggcgggccg gtcaatgcgc
    11341 tgttcgcgtc ggcggatgag gccatccgat cagtgcagca ggcggctgcc ggcgcgctgc
    11401 gcgaagccgg tatgaagtgt gctgatgtgg ccgtgctcta cgcgagtgct cccggggctt
    11461 cgcgcgatat cgtgagcgct ggaatcgatg ggctagtctc tccggctagg cttgagacgg
    11521 caggagacga gatggcggtc ttgacaggtg ccctcgtgga cgagcccgga gtcgtggtgc
    11581 tggccggcac tggttcgttc gccgtcgggc gcggcatacg ccgagactgt gacagccagg
    11641 ccggcgatac gggtcggcac agagtcgtaa cccggtacag aatcgcaacc cggggcggat
    11701 gggggccttt gcttggcgat gagggaagcg cgtacgccat cggcctcgcg gcactgcgcg
    11761 ctgcagcgct ggccttcgat aaccgaggtg agcacacatg cctgtgcgac atggctctag
    11821 agcacttcga tgctcctcac gcgtggtcgc tggccacaat gaacatgagc cgcgagcaga
    11881 tcgcttcctt cgcagttcgg gtgtcgcagg cgtcggctgc gggcgatgat gtcgcgtcgg
    11941 cgatacttgc gcaggctggg cgggatctgg gcaggcttgg agtgagtgtg ctgcgggagt
    12001 tggctgagcg gagcgcgacg ggtgagccgg gtgagccggt tgagcccgtt gagcccgttg
    12061 agcccattga gcccgttgag tcgggcaatg ccgggcatgg gcgcaggatt ctggcggtct
    12121 tgaccggtgg tgtatcccgg gcagccttac cgctgatcgc ggcgtttcga gatgagattc
    12181 tgtctttttc acccgatgct cagatagtgc cgccgcagtt ctcgcccgcg ggaggcgcgc
    12241 ttcttgtggc gctggagatc gcggagctga gggaaccggg catggtttca gccggcctgc
    12301 gcgcatcggc cgaagcatgc tctgaattgc gggcttagtg gacttggcac ctctggcgag
    12361 tcagtcggtt gttgtatcaa tcagtggagc tgtcagtctg ctcaaggagg actggtgaac
    12421 ttgctcggaa aaggcgcagc gtatcttgat gagataagga gtcttctaga caagatccag
    12481 actactcagg ccgagaacat cgcggaagca gcagacatga ttgttgacgc ggtgagccat
    12541 ggcggggcgg tccatgtgat ggataccggc cacatgctca tgcacgagct attcggcagg
    12601 accggcgggc tcatgatgct ccgcccggtc aacatcacca tggacgttca gaaccctggc
    12661 cctgcccgcg caggcaaggt gaagcccaag gtgtatctgg accaaattcc gggtcttccc
    12721 cagtttgtgt tggatcgaag cgacatcttc gcaggagatg tgttgattat cggctcggta
    12781 tcgggcaaga acacccttcc cgtggggctg gcattgctcg cccgcgaaca gggggtcaag
    12841 gtgatagcgc tcaccagcgt ggcgtattct gctgctcttc agggtgagca tccatcgggc
    12901 aagcgccttt tcgaagctgc cgatgtggtg ctcgacaact gtggcattgt gggcgacgcc
    12961 gcgctcgata tagagggtat cgatgcgaag gtgggcccca cgtcgggcat cgcagctgcc
    13021 gccatcatgt gggccctgga gatggagatc atggaacgga tggcccggcg cggcatgaag
    13081 cccagtgtgt ggctgagcaa tcacatgccg ggagcgggcg agttcaacag acgctcacgc
    13141 gaagagtgcg acaagcgggg gtattgacgc cgggtgtgtt ggcgcacggc gtctcggcac
    13201 cctaatgcct ggatgttctg atgcctagat gccctggcac ctcgaggccc tggcgcttcc
    13261 aggccctggt gcccctgaga tacgcgtcat tgtcgcagcg acccgacttt tggacgcgag
    13321 tgaggagaga cggctacatg gcgacttccc tttcggattc ggctttatcc cctactctgc
    13381 catcaacagg cagcagacgg cggcggaggc gcgaaacgct gacagcgtgg tctttcatac
    13441 tgccggcagt cataggcctt gggatctttg tgtatggagc gctcgtgtac tcgctgtata
    13501 tatccttcac tagttgggac ctgctcacgc ctgcgaaatg ggtggggttg gccaactacg
    13561 cgcgcctatt ccgcgacgag tcgttccacc agtgcatgta caacactctc tacttcgtcg
    13621 tcgccatagt gccggccggc atcgctctgg cgatggccat ggcggttgcc ttgaaccgac
    13681 cgctcaaagg cgttgcgttc tttcgggcgg catactacat gccgtcgatc acctccacag
    13741 tcgccattgg gctggtatgg ctgtggatct tcaaccccga tcaggggatt ctcaacacca
    13801 tattgagggc gcttggcgct accaacccgc cgcgctggct cgagagtatg agatgggcca
    13861 agctcgggct gtcgatcatg cgattatggc aggtgtcggg ctactacatg atcatgtacc
    13921 tggccgggct gcagaatatc cccaaggagc tgtacgaagc ggccgagaca gatggcgcaa
    13981 ccccgtggca aaagaccagg cacatcacgg tgccgctcct gtcaaatacc acattcttcg
    14041 caactgtcat gctcttgatt gaggttttca acctgttcga ggcagtatac gtcatgactg
    14101 aaggaaggcc gggcggctca actaacacag tcctttacta catttataca gaggcattcc
    14161 agtcatacag aatgggctat gcgtccgcca tagcctgggt cctgtttgtc atactgttcg
    14221 tcgctacttt ggtccagttc atggtgaggc gccggaatga gcgagttggt tagcgagtcg
    14281 gttaaggtgg tgatcctgtg aaagtcaaaa tccggcctga tcaggttgtg ttgtacgtag
    14341 tgttggtttt gctcgcagca atcatgatat acccgtttgt gtggtcggtc acggcatcgt
    14401 tcaaaacaaa ccgccagata tttagcggga atcctctgaa cctgattccc catccggcca
    14461 cgctcgacaa ctacaggcag gcctttgcgt tgatgccgtt cggcaggttc ctgctgaact
    14521 ctgcgatgct ggccattgtg gtgccggccg cctcgatact gattggttcc atggctgcat
    14581 acgcattcgc cagaatggac ttcactgggc gcgacttcat ctttctggcg cttctgggcg
    14641 tcatgatgat gcccgggcac atcacactca tcccccggtt cgcgctcgtt cggaagctcg
    14701 gatggatcaa caagtacacc gccttgatca tacctgccat attcagcgga caattggtgt
    14761 tcaacatatt cctcatgcgt cagcacttct tgagtgttcc gaaggagctg gaagaggcgg
    14821 cgctgattga tgggtgttcc cgctggggcg tgtggtggaa ggtggtcatg cccaactcta
    14881 gacctgccat cgcaacgctg gcaatcatcg gcttcaagag cgagtggaac agcttcctgt
    14941 ggcctaacat cgttatcaac gactacatga agatgcccat acaagtggga ctgagctgcc
    15001 tgcagagcaa tatgcgcaca gggtgggatg tccttatggc cgggactact atcgccatca
    15061 tccctctcat aatcatattc ctctgcttcc agcggttctt tatctccggc gtcatgactt
    15121 cagggatggg gggtaggtag acgggcagct agacgggtgt gcgattccga ctgccactgc
    15181 gagtacgtag atgtagttac ggttacagcc aggctttgat gcctgaacac actacaaggg
    15241 aggcccagtg caaatgagac gaatctctgg tagggtaggt ctgatctgtt tggcggcgct
    15301 tctcgtcctg tcgatgagcg ttgccgcgtc gcccaaggcc gcgcgcacca aagtgcaggt
    15361 gatctcatgg tgggacttca caaactcccg cccgctcatc gaactcaagg ccaagttcga
    15421 ggagctcaac cccgaccttg agctggacta tatccgtatc ggaactggat acgccgacaa
    15481 gatgttggtc atgatcgccg gcggcgcgga tcttcccgac gtcatgatga tagccatgga
    15541 caaggtgccg attttcgccg acaagggcgc catcatgaag ctcgacaagt acatcgagtc
    15601 cgactacaaa gccgagatgg cgaagcttta tcctgtggtt gccgacgccc ttacagtgaa
    15661 gggcagttac tacgccatgc cgcgcgatat caccagcaaa gtgatgttct tcaacaagca
    15721 actctttgcc gaggccggtg ttgccattcc cggtccggac tggacatggg atgacttcaa
    15781 agcgcttgcc aagaagatga cgcgcgatac gaacaaggac ggcgttactg atcagtgggg
    15841 attctacttc cccaagtaca ccgacggctg gactgactgg ctgatgcaga atgagggcgg
    15901 cttggtgacc aaggagggcc agtcccttct
//
LOCUS       DAOTAC010000014        15739 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_3119_length_15739_cov_4.440704, whole genome shotgun sequence.
ACCESSION   DAOTAC010000014 DAOTAC010000000
VERSION     DAOTAC010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 15739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15739
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3119_length_15739_cov_4.440704"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1070
                     /locus_tag="PLM74_01225"
     CDS             <1..1070
                     /locus_tag="PLM74_01225"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HON41738.1"
                     /translation="GIVLTHGHEDHIGALPYVLKRLNCNIPVYGTRLTLALVESRLKE
                     HGLRLQSYAHVVTAGDSIRLGSIGVEFIHVSHSIADCAALAIDTPAGMIIHTADFKFD
                     QTPVDGRVMDIRRFADLGARGVLVMLSDSTNADRPGYTMSERVVGDIFRNAFRDAPSR
                     IFVTTFASHIERIQQVLDAAAESGRRVAIVGRSMENNVRLALDLGYLHVEPGTLVDLD
                     TLDSLPPNQVVIITTGSQGEPMSALTRMAMSDHRKISIVPGDTVIISASTIPGNERMI
                     GSTINRLFKQGAEVIYDAFSGFHVSGHASAEELKLMLNVVRPQYFVPVHGEYRHLVKH
                     ARIAQELGMSRRTYSYRNLVT"
     gene            1070..1474
                     /locus_tag="PLM74_01230"
     CDS             1070..1474
                     /locus_tag="PLM74_01230"
                     /inference="COORDINATES: protein motif:HMM:NF036802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41739.1"
                     /translation="MEFTRQSAHVVGRVASGHLLVDGFSVGTVGNVVLRDRRLLAEDG
                     IVVAVVAADRGREMVVGDVEIVTRGFVYVKEAETFIEEMRSVGGKAIARHTGEGSLEV
                     QSLKDQLRHAISSFIFERTGTRPMVIPVVIDV"
     gene            1533..2276
                     /locus_tag="PLM74_01235"
     CDS             1533..2276
                     /locus_tag="PLM74_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HON41740.1"
                     /translation="MRVESEVGPSVREAEVRQPAASPIKEYGEIATPAPPASNIHVLS
                     IVGQIEGHMVLPPQNKTTKYEHIMPQLVAIEQSPEIEGLLILLNTVGGDVEAGLAMSE
                     LLAGMRKPSVSIVLGGGHSIAVPIAVSCSYSIIASTATMTIHPLRLSGQLVIGTQQTY
                     EYLERMQERVIKFVAEHSRCTPESFRELMFRTGELLRDVGTVLVGQDAVDAGLIDSVG
                     SLSDALDKLDELISIRRKISGGKDWGKVH"
     gene            2276..2503
                     /locus_tag="PLM74_01240"
     CDS             2276..2503
                     /locus_tag="PLM74_01240"
                     /inference="COORDINATES: protein motif:HMM:NF025401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlzJ-like family protein"
                     /protein_id="HON41741.1"
                     /translation="MLYTIIPLEEVFAAPPWAAGGGDSAGAPPLPVPAVVGGVQVLVS
                     PSYDGRNRVERVISTDPAVYLDPRFAPGTLI"
     gene            2607..3602
                     /locus_tag="PLM74_01245"
     CDS             2607..3602
                     /locus_tag="PLM74_01245"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HON41742.1"
                     /translation="MITAIQFAGGLSLFLVSLVCMSAALGRIIGPRTRAALSGASVPA
                     AFVTGLAATALVQSSTLVSVTLVGLVHAGAMGLGPGFAAVLGANIGTTVTAQIAGFRP
                     VWIGRIAAAAGAVLMALAMRDRAGGRKTSRAFGGVASFGFAGIVSGLSVMAGACAKTF
                     DSDALKLILAMASRNRVLAAAVGAAATAAVQSSALITALLVTLTREGLIQLPGAVALV
                     IGSNVGTCLASLAASLPTGREARALAWANIVFNMLGAAAVLPFTEPLSRLVALTSADP
                     GVQLANAHALFNIITAAAVLPFARQFASLFVGRTEERSSIDNVPSHRVRSHSRPY"
     gene            3553..4398
                     /locus_tag="PLM74_01250"
     CDS             3553..4398
                     /locus_tag="PLM74_01250"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HON41743.1"
                     /translation="MTTFQAIVLGVIQGLTEFLPVSSSGHLALAQMIMGIEPSVTFAL
                     GVHAGTALAVLALYGRDVWRIIVGFFRGIAHRDGSARLALCLVFTSIPAAIVGFAASD
                     AVEAAFGSPVFVAIGLLISGIVLWVTGSRAASAPGPASASGRLARRSVTERTITYGQA
                     LGVGAAQAIAVFPGVSRSGMTISGALGLGFDRSFAAAYSFIASLPVILGAVVLWPVSN
                     PNALSSLHLPSLAAGAIASFVSGMAAMIILRRIVQHGKLRRFSYYLWAVGLAVILWEA
                     RGLLL"
     gene            4436..>5219
                     /locus_tag="PLM74_01255"
     CDS             4436..>5219
                     /locus_tag="PLM74_01255"
                     /inference="COORDINATES: protein motif:HMM:NF024882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HON41744.1"
                     /translation="MTARRARRPARAAGRAKRQKTRAKPSSEERALEGNLGVEIGGIS
                     LLAAAGVGLVSLVRGPAGYVGGALAASLLSALGRGAYVVPLIAALTGILMLWRRTGYA
                     VMVRLVGLVVASSAAVVSFELAVADGAPLALPFSDYLRNSGVLGGGAVGAAGAWVFMR
                     LFGYAGTVIVLCAMGAGGLAVATNVSVASAVTWVARTVWSFLWLVVRMVRAGFDAIVL
                     YLFEESAPSPDAPRETGAPGGGPDDLSRSTPRRRRSVPSTAPA"
     assembly_gap    5220..5319
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5560..7062
                     /locus_tag="PLM74_01260"
                     /pseudo
     CDS             5560..7062
                     /locus_tag="PLM74_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118764.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
     gene            7088..7534
                     /locus_tag="PLM74_01265"
     CDS             7088..7534
                     /locus_tag="PLM74_01265"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HON41745.1"
                     /translation="MKEIGELLREARESCGMTLQQASEITKVRTKYLEAIENGEFAVI
                     PGEVYLKGFIRTYADCLGLDGTQVIAEYNELRSVRDSAALRQLEQERIGRMKRTREES
                     KQRRLHALGKGILIALIVAAVALVVYYAGRSMGLIKWAPPWLKWLR"
     gene            7553..9778
                     /locus_tag="PLM74_01270"
     CDS             7553..9778
                     /locus_tag="PLM74_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41746.1"
                     /translation="MRTVGDLIDDLGRLDGPRFDETVFELFHRGPRALDRLAAYWESG
                     ASGETQSKIRLSVRSQWTRIQEVLAGMAPRALGSAGPRLAISLGSADSLARRAQRCID
                     EGRLDTAAAIASALAASGADGVEQALLEARIAMAVGRPDQAMTCLGGYCQTTPYPGSH
                     SMAREWFARAALCAGRSVEAFVSRLHLEPDVGAMSEIVMSLVDADDGAGLGSCVAHVA
                     RRGDPELWTLLAESAAAALGDAMGIDTDLVANLLGMGSEIESLLDTIESVVTDMESSM
                     PAPAERSMAAELVSLASDALSMLESAHSLHLAVSGRSVDEGNDISETISLLLGVADPP
                     PRCNHDSHSCEWTWTFRERPSEDALGRARLAALMCGASWNALWRNGEYVVSVSGSALQ
                     PLASGSAGASASASSWAPASGPDASEIERMLKSLPSQEAAELLIRAIRSARVHHARGL
                     LDEACRALSEMAGALGAEVGVVSEPALALDGVRRAVAKIRAEIERWSRYATEASCMSR
                     DSNADAHAGAQVGAHIGGDANANAEDQPSVADADEWSLPDELDCTPEAYQALRTAHLL
                     IAGLSRNESLPPEQAELPAFLIQKAVQMEAERKLGEALAVHRLKPRALAATRDRGKRR
                     RELDPDFVRDAVSRAPQGINPDRVRGGLERLFARVLDGSSRPLSGDLFLAGLALAYLD
                     PLGDAERSAALGSAFLAMDEAAVAKAASDMPERRDLRRLEQAGIEAIRALSLFGGREG
                     R"
     gene            9786..10733
                     /locus_tag="PLM74_01275"
     CDS             9786..10733
                     /locus_tag="PLM74_01275"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HON41747.1"
                     /translation="MEYGIGRVSGIDIESLVELFNDAYSNYYVDIQVNPARLHGLIER
                     EDIKVDNSFVAYEGQLPVGVVFLGVRGRRGYICGMGVRTSRQRRGIGELLMRQALAEA
                     RRLGLSSVQLEVVAANRRAIALYAKLGFVPMRNLGLWERNTPPPPAPASAPKSAPTPA
                     LGARATRACRDEAHVPSQSGVRVGAVRMAGVLPLVDAFNEIRPCWQNEAQSLVKMSDR
                     FVGYVSHLDSAATAYVVFGDSASGLYIADFAAAPRLGNAERQSMCEILLSAVDRDVPR
                     ASAKAFNIPLQGYQEAVLRARGFDLTLIQQEMVLCLKWR"
     gene            10715..10984
                     /locus_tag="PLM74_01280"
     CDS             10715..10984
                     /locus_tag="PLM74_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HON41748.1"
                     /translation="MLKVALTTFATVFLAELGDKTQLATMLLVAQGKSPLAVFLGSAS
                     ALVVSSLVGVLAGGVIAKYIPEIWVRAAAGSGFIILGVLLLLGRF"
     gene            11014..11802
                     /locus_tag="PLM74_01285"
     CDS             11014..11802
                     /locus_tag="PLM74_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41749.1"
                     /translation="MKTRMLVLAVLAAVMAISATLSVWAFPVCAAGLPSLEERITAAD
                     LHWSRRSQAGVTEALLQNLEDADRDYPNQGEVLWRLSRAYWWKSTRIAKDDKKTRIEW
                     LDKAKATGERAAKAAPANPNAHYWYAAAMAETGVARGILQSLFMVKPTREALERGLAA
                     DPNHASCHYLMAELYLQLPGPPLSIGNKQKAVEEARLAVKYGPDSSSHWLVLGRALIA
                     TKQYGEARQALGTLLAMAPDQGDPDGTRADQDEGRAELQSIANK"
     gene            11830..13137
                     /gene="rimO"
                     /locus_tag="PLM74_01290"
                     /pseudo
     CDS             11830..13137
                     /gene="rimO"
                     /locus_tag="PLM74_01290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01125.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
     gene            complement(13748..14998)
                     /locus_tag="PLM74_01295"
     CDS             complement(13748..14998)
                     /locus_tag="PLM74_01295"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013932624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HON41750.1"
                     /translation="MAGSQYEQVLARLREASRILNLEPGIFEILAEPERTLEVALPVE
                     MDDGSTKVFMGYRCQHSTVRGPAKGGVRYAMDVCMDEVKALASWMTWKCAVAGIPYGG
                     GKGGIRVDPMKLSQNELKRLTRRYVYAILPIIGPEKDIPAPDMGTNAQIMGWFVDTYS
                     VLTGVLSPGVVTGKPIPIGGSLGRREATGRGVVFAVRELLARLGKKPSEITCAVQGYG
                     NVGSVSAELAHELGCKVVGVSDVTGGVYNPAGLDVPEISKYVAEHKFIEGYTAPGASI
                     ISNEQLLTLDVDLLIPAALQGQITEANADKIKAKLIVEGANGPTSAEADKMLEEKGVI
                     VVPDILANAGGVVCSYFEWVQNLQNFYWTEEEVNTRLDGIMTRSFAEVWDFAKQRDLS
                     MRTGAYILSIAKVAEAIRMRGIFP"
     gene            15196..>15739
                     /locus_tag="PLM74_01300"
     CDS             15196..>15739
                     /locus_tag="PLM74_01300"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HON41751.1"
                     /translation="MKRTLSLLLAAGMLLALALPVFSAPAPKVGIVFDIGGLGDQSFN
                     DAAYRGLKRLESELGATTTYIESRTPSDYEPNLATLARQGYDVVWGIGFLMADAVKKV
                     SQQFPNTRFGIIDNGYSDEEYKAMKNVLGVLFKEQEGSFLVGVLAALTTKTNIVGFVG
                     GMDFPVIERFESGFKAGVWKT"
BASE COUNT         2874 a         4378 c         5167 g         3220 t
ORIGIN      
        1 gcgggatcgt gctcacgcat gggcacgaag atcacatcgg tgcgcttcca tatgtgctga
       61 agcggctgaa ctgcaacata cctgtttacg gcactcgcct gacattggcg cttgtggagt
      121 caaggctgaa ggagcacggc ctgaggttgc agtcgtacgc acacgttgtg acggcgggcg
      181 actcgatacg cctgggcagc atcggcgtgg agtttatcca tgtcagccac tccatcgctg
      241 actgtgcggc cctggccatc gacacgcctg ctggcatgat tatccatacg gccgacttca
      301 agttcgacca gacgccagtc gacgggcggg tcatggatat ccggcgcttt gcggacctgg
      361 gcgcacgcgg cgtactggta atgctgtctg actccacgaa tgcggacagg cctgggtaca
      421 ccatgagcga acgggtagtg ggcgacattt tccgcaacgc attccgcgac gcacccagca
      481 gaatattcgt gacgacgttt gcgtcgcaca ttgagcgaat acagcaggtg cttgatgcag
      541 ctgcagaatc gggccgtcgt gttgcgatag tcggtcgcag catggagaac aacgtgaggc
      601 ttgccctgga tctggggtat ctgcatgttg aacccggtac tctggtagac ctcgacacgc
      661 tggactccct gccgccgaac caagtcgtga tcatcaccac gggcagccaa ggggaaccaa
      721 tgtctgccct gacgcgcatg gccatgtccg atcacaggaa aatatcgatc gtgcctggcg
      781 ataccgtgat catctctgcc agcaccatac cgggcaatga acggatgata gggtcgacca
      841 tcaatcgtct tttcaagcag ggagcagagg taatttacga tgccttttcc ggattccacg
      901 tttcggggca cgccagcgcc gaagagctca agctgatgct gaacgtggtg aggccgcagt
      961 acttcgtgcc ggtgcacggc gagtaccgac acttagtcaa gcatgccagg atcgctcagg
     1021 aactcgggat gtcaaggcga acatactcat accggaactt ggtgacgtga tggagttcac
     1081 ccgccagtcg gcgcacgtcg tagggcgcgt cgcctccggg catcttctgg tggacggctt
     1141 ttctgtgggc actgtaggca atgtagtgct gcgcgacaga aggcttcttg cagaggacgg
     1201 cattgttgtg gcggtggtcg cagcggaccg cgggcgtgag atggtcgtag gggacgtgga
     1261 gatcgtcacg cgcgggtttg tgtatgtaaa ggaggccgag accttcatcg aggagatgag
     1321 gtcggtggga ggaaaggcga tcgcgcgcca taccggcgag ggcagcctgg aggttcagtc
     1381 gctcaaggat cagctgaggc atgccatatc gtctttcatt tttgagcgca ccggtacacg
     1441 gccgatggtg attcctgttg tcatagacgt ataagcatgt cttgtccacc ctcatactaa
     1501 ccgtattgag tcaaacggag gtgcgggtga tcatgagggt ggaatctgaa gttgggccca
     1561 gtgtgcgaga ggccgaggta cggcagccgg ctgcaagtcc tataaaggag tatggggaaa
     1621 ttgcgacccc ggctccgcct gccagcaaca tacatgttct gagcatcgtg ggccagatcg
     1681 aagggcacat ggttctgcct ccgcagaaca agaccacgaa atacgagcat atcatgcctc
     1741 agctcgtggc aatcgagcag agcccggaga tagagggcct gttgatcctg ctgaacactg
     1801 tgggcggcga tgttgaggca gggctcgcta tgtcggagct gcttgcaggc atgcgcaaac
     1861 cctcagtaag catagtgctg ggcggaggtc actccattgc tgtgccgata gccgtgagct
     1921 gtagctactc aattatcgct agcacggcca cgatgacaat ccatccgctg aggctctcgg
     1981 gccaactggt catcggcaca cagcagactt acgagtatct cgagcgtatg caggagcggg
     2041 tcatcaagtt cgtcgccgag cattccagat gtacaccgga gagcttccga gagctgatgt
     2101 tccgcaccgg cgaactcctt cgcgatgtcg ggacggtgtt ggttgggcag gacgcagtgg
     2161 atgcaggctt gatcgactca gttggaagcc tgtcggatgc cctcgacaag ctggatgagt
     2221 tgatatctat cagacgcaag atatctggcg gaaaagactg ggggaaggtg cactgatgct
     2281 ctacacaatc atccctctcg aagaggtgtt tgcggcgcct ccatgggctg ccggcggcgg
     2341 cgactccgct ggtgcgccgc ctctcccggt tccggcagtc gtaggcggag ttcaggtgct
     2401 ggtttcgccg tcgtacgacg gcaggaaccg cgtggagcgc gtcatctcga ccgaccctgc
     2461 agtctatctg gacccgcggt ttgcccctgg cactctgata tagcctgtag caggagtgcg
     2521 gtagccaccc ctcggtacgc tacataaccc tctgcccggc cacatacgtt tgtgtgacta
     2581 cgtgttgccg gggcggggtg cggctgatga ttacggcaat tcagttcgct ggagggctgt
     2641 cgctgtttct cgtgtcgctg gtgtgtatga gtgcggcgct ggggcgtatc ataggaccca
     2701 gaacgcgtgc tgcgctttcc ggagcgtctg tgcccgcagc cttcgtgacc gggcttgcgg
     2761 ccactgccct ggtgcagagc tccaccttgg tcagcgtcac actcgtagga ttggtgcatg
     2821 ccggcgccat gggcctgggg ccaggatttg cggctgttct gggcgccaac atcggtacca
     2881 cggtgaccgc gcagatagcc ggattccgtc cggtttggat tgggcggata gcggctgcgg
     2941 caggcgcggt tctcatggcg ctcgcgatgc gggatcgagc gggcggacgc aagacgtctc
     3001 gcgcttttgg gggcgtggcg tcattcggat tcgccgggat cgtttccggc ctgagtgtca
     3061 tggcgggggc gtgtgcgaag accttcgact cagacgctct caagctgatt ctggcaatgg
     3121 catcccgcaa cagggtactt gcggcggctg tcggcgcggc ggcgacggca gccgtgcaga
     3181 gcagtgccct cataactgcg ttgctcgtga cgttgacccg tgaggggctg atacagttgc
     3241 ccggcgcggt cgcactggtc ataggaagca atgtagggac atgcctggcg tcgctcgcgg
     3301 cctcgctgcc tacaggcagg gaggccagag cgctggcatg ggccaatatc gtgttcaata
     3361 tgcttggagc ggcggcggtc ctgcccttca ccgagccctt gtcgaggctg gttgctctta
     3421 cgtctgccga cccaggtgtt caactcgcca acgcccatgc gctgttcaac atcattactg
     3481 cggccgcagt ccttccgttt gcgcgccagt ttgcgtccct atttgtcggc cgcaccgagg
     3541 agcgatcgag cattgacaac gttccaagcc atcgtgttag gagtcattca aggccttact
     3601 gagttccttc cggtatcgag ttcggggcac ctggccctag cacagatgat catgggcata
     3661 gaaccttcgg tgacgttcgc gctcggcgtg catgcgggaa cggctctcgc ggttttggcg
     3721 ctttatggcc gtgacgtatg gcgcataatc gtcggcttct tcaggggaat agctcatcgt
     3781 gacggcagcg cccgattggc gctgtgcctc gtattcacca gcattccagc ggccatagtg
     3841 ggcttcgctg caagcgatgc agtggaggcc gcattcggct ctcccgtgtt tgttgctatc
     3901 ggcctgctga tatcggggat cgtactgtgg gtgacgggca gtcgtgcggc ttcggccccg
     3961 ggtccggcct cggcctcagg tcgcttggcc cggcgatccg ttaccgagcg tacaatcacg
     4021 tatggccagg ccctgggagt tggggccgcc caggcgattg cggtgtttcc gggcgtctct
     4081 cgctcaggga tgaccatttc aggggcgctc ggcctcggct tcgacaggtc gtttgccgct
     4141 gcttactcgt tcattgcgtc gcttccggtg atcctgggcg ccgtggtgct ctggccggtt
     4201 tccaacccga acgccctttc gtccctgcac cttccgtcat tggcggcggg agcgatcgcg
     4261 tccttcgtat caggaatggc tgccatgata atcctcaggc gcattgtgca acacggaaag
     4321 ctcagacggt tctcatacta cctctgggcg gtaggcctcg cagtcatcct gtgggaagct
     4381 agaggattgt tgctctgaga gtagaattga ggtcgtgagt aggtggtgag catgtgtgac
     4441 agcacggcga gcccgtcggc cggcccgggc cgccggaagg gctaagaggc agaagacgag
     4501 ggccaaaccg agcagcgagg aacgggcgct cgaagggaat ctgggcgttg aaattggagg
     4561 aatatcgctt ctggctgctg ccggagtggg gctcgtatcg ctcgtgcggg gcccggccgg
     4621 atacgtggga ggcgcgcttg ccgcatccct gctgagcgct ttggggaggg gcgcctatgt
     4681 cgtccccttg attgcggcac tcactgggat actgatgctc tggcgtcgta caggctatgc
     4741 ggtgatggtg cgcttggtcg gtctggtcgt tgcgtcttcg gcggctgtgg tatctttcga
     4801 gctggccgtg gccgacgggg ctcctcttgc attgccgttt tccgactact tgcgcaactc
     4861 gggcgttctg ggcgggggag ctgtgggcgc agccggagcc tgggtgttca tgcgcctttt
     4921 cggctatgca ggaaccgtaa tcgtgctgtg cgccatgggc gccgggggtc ttgctgtggc
     4981 caccaacgtg tcggtggcga gcgccgtcac ctgggttgcc cgaactgtct ggtcttttct
     5041 gtggcttgtt gtcaggatgg tgagggcggg gttcgacgcg atcgtgctct atctgttcga
     5101 agagagtgcg ccgtcgcctg acgccccgcg cgagacggga gcgcccggcg gcgggcctga
     5161 tgatctttca cgttcgaccc cgcgccgacg gcgttccgtg ccatccaccg cgcctgctgn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc tttcacgttc gaccccgcgc
     5341 cgacggcgtt ccgtgccatc caccgcgcct gctgtaccca ttgcgcctgt gggcccctct
     5401 ccggtcgatg cacccactcc tgcggattcg cattcggcgc cagttcagcc tatcgcgggc
     5461 ggggctgcta tacgggccgt ggcctctgca ccgacatcag aggggtccgg catgccgctg
     5521 cagcagctga agctgatcga cgatagcgcg acgggaattc ttcctccact gtcgatcctc
     5581 agatcgccgg cgcgccgtcg ggcgggccgg aaggaatcgg aggatccgaa cgcgcttggg
     5641 cggatgttgg aagagacgct tgccagtttc ggtgttgacg ccactgtggt cggagtaagt
     5701 cagggtccca tggtgaccag gttcgaggtc cagccgggcg tcggggtgaa agtgtcgaaa
     5761 attgttaacc tcgctgacga tctggctttg gctttcgcat ccgccggcgt gcgcattgag
     5821 gcacccatac ccggcaagtc cgctgtggga atcgaggtgc cgagccgaga gaagtccata
     5881 gtctacttgc gcgaggttct cgactccgac gagtttcgtg gggccgagtc tcgtcttact
     5941 atggcccttg gcaaagacat tgcgggtgct cccatagtgg ctgacctggc ggacatgctc
     6001 catctgctgg tcgccggcgc caccggctcc ggcaagagcg tatgtctgaa ctcgataatc
     6061 tgcagcctgc tctttaaggc tacgcctgaa gaggtcagat tgctgatgat agaccccaaa
     6121 cgtgtggaac tctcggtata tgacgggatt cctcacctcc tcgccccggt gatcaccgat
     6181 cccaagaagg cttcgggcta cctgcggtgg gttgttgagg agatggagaa cagataccgc
     6241 cttttccaga tcgcaggagc cagaaacatt gctcagtaca acgaaatggt caagtcgggc
     6301 actctggagc agttcctgcc tgatcaggag acggaggctg aaacccaggc tcaagctaag
     6361 cagacccagg gccaggctga ggccgagcga cctcggccgc tgtcgtatgt cgttgtgatc
     6421 gtggacgagc tggccgactt gatgatggtg gcgcaacgcg aggtggaaga tgctattgcg
     6481 aggctcgctt tcatggctcg cgccgccggc atacacttac tcatagcaac acaaaggccg
     6541 tcggtggaca tcctgacagg gataatcaag gccaacatcc cgtccaggat cgcttttgct
     6601 gtttcgagcc aggtggactc gcgcatcatc ctcgatacgg ccggcgccga gcgcctgcta
     6661 ggcaagggcg acatgctatt ccacccggtg ggactgccaa agcccatcag ggttcagggc
     6721 gcctacatct ccgacgaaga ggtagcggtg ctggtggagt tcgtgcggcg cgggggcaaa
     6781 ccgctctatg aagcggagag tgtagagtcc tcagcgaagg ctggggaagg gatagccgag
     6841 gatgacgagc tgtttgcgga agcggctacg ctggtggttc agaccggcga ggcgtccata
     6901 tccaagatcc aacggaggtt cagggttggg tacgcgcggg cggcgcggct gatcgacacc
     6961 atggaagtgc tgggcatcat aggcccatac gagggcagca aaccccgaaa ggtgctaatg
     7021 gacgaggcgg aactggagga agcgcttggg cgtcgcggat gacaaacata cgcgggaggt
     7081 gccgtctttg aaggagatcg gcgagcttct aagggaggca cgcgaatctt gcggcatgac
     7141 attgcagcag gcaagcgaga tcaccaaggt tcgcactaag taccttgagg ccatcgaaaa
     7201 cggcgagttt gccgtgatac cgggggaggt gtacctcaag ggtttcatac gcacttacgc
     7261 cgactgtctt ggccttgacg gcacgcaggt gatcgcggag tacaatgaac tgcggagtgt
     7321 gcgcgactcg gcagcactgc gtcagctgga gcaggagcgg atcgggagga tgaagcgcac
     7381 ccgcgaggag agtaagcagc gacgcctgca cgcgttgggg aagggtatcc tgatagcgct
     7441 gatagtggcg gcagtggcgc ttgtggtcta ctacgccggc cggtccatgg ggctcatcaa
     7501 atgggcgccc ccgtggctta agtggcttcg gtaagcgaaa gaaggtcagc ctgtgcgcac
     7561 tgttggggat ctcattgacg atctgggccg ccttgatggg ccccgattcg atgaaaccgt
     7621 attcgagctg ttccaccgtg ggcccagagc ccttgatcgg ctggcggctt actgggagtc
     7681 gggtgcatcg ggcgagaccc agagcaagat ccgtctctcc gtacggagcc agtggacgcg
     7741 catacaggaa gtcctggcgg gcatggcgcc tcgcgccctc ggctcggccg ggccgcgact
     7801 ggccatttcc ttggggtcgg cggattcact ggcgcgtcgc gcacagcgct gcatagatga
     7861 gggcaggctg gatacggcgg cggcgatcgc atcagccctg gcggcgtccg gcgccgacgg
     7921 ggttgagcag gcattactgg aggcgcggat cgcgatggct gtcggtcggc cagaccaggc
     7981 aatgacctgt ctaggcgggt attgtcagac cacgccgtac cccgggtccc atagtatggc
     8041 tcgggaatgg ttcgcgcgcg cggccctgtg cgccggtcgg tcagtcgagg ccttcgtgtc
     8101 caggctccat ctggagccgg acgtgggcgc aatgtccgag atcgtcatgt cgttggtgga
     8161 cgcagatgat ggtgctgggc ttggatcctg cgttgcacac gtggcgcgcc ggggcgaccc
     8221 ggagctatgg acgctgctgg ctgagtcggc tgcggcggcg ctcggcgatg ccatgggcat
     8281 cgacaccgac ctcgtggcga atctgctcgg gatgggcagc gaaatcgaga gcctgctgga
     8341 tactatcgag agtgtggtca ccgacatgga atcgtcgatg cctgcgccgg cggagcgatc
     8401 catggccgca gagcttgtgt cgctggcatc tgatgcactt agcatgctgg agtccgccca
     8461 tagtctgcac ctagcggtca gtggccggtc ggtcgacgaa ggaaacgata tctccgagac
     8521 catatcgctg cttctgggcg ttgcggatcc tccgcccagg tgcaaccatg atagccacag
     8581 ctgcgaatgg acgtggacct tccgtgagcg tccgtccgag gacgccctgg gccgagcaag
     8641 gctcgcggcg ctgatgtgcg gggcttcgtg gaatgcgctt tggaggaacg gagagtatgt
     8701 tgtgtcggtg tcgggttcgg ccttacagcc gttggcgtcc ggctcagccg gtgcctctgc
     8761 gtccgcctca tcctgggctc cggcgtcggg cccggacgct tccgagatag agcggatgct
     8821 caagtcgttg ccgtcgcagg aggctgcgga gttgctcata cgtgccatcc ggagtgcgcg
     8881 ggttcatcac gcacggggtc tactagacga ggcgtgccgg gcgctgtcag agatggcggg
     8941 ggcgctcggg gccgaggtcg gtgtggtatc tgaacccgcc cttgctcttg atggagttcg
     9001 gcgtgcggtc gccaagatcc gggcggaaat tgaacgctgg tctcgatatg ctacagaggc
     9061 atcctgtatg tcgcgggact ccaatgctga cgctcacgca ggcgctcagg taggcgctca
     9121 cataggcggc gacgccaatg ccaatgcgga agatcagccg tccgtagcgg atgccgatga
     9181 atggagtctg ccggatgagc ttgactgcac cccagaggcg tatcaggccc ttcgaacggc
     9241 tcatctgctg atcgccgggt tgagccggaa cgagtcgctc ccgcctgagc aggcagaact
     9301 gcccgcattt ctgatccaga aggccgtgca gatggaagca gagcgcaagc tgggagaggc
     9361 tctggcagtg caccggctga aacctcgggc gctcgcggcc acccgcgacc gtggtaagcg
     9421 tcgccgcgag cttgatcctg atttcgtgcg tgatgccgta tcccgcgcgc ctcagggtat
     9481 caatcctgac agagttcgcg gcggtcttga gagactgttc gcccgtgtac tggacggcag
     9541 ttcgcgaccg ctcagcggtg atctgttcct ggccggcctg gctttggcat acctagatcc
     9601 cctgggagat gcggagcggt cagcagctct ggggtcggct ttcctggcca tggacgaggc
     9661 ggcggtggca aaggcggcaa gcgatatgcc ggagagacgg gatttgcgca ggctggagca
     9721 ggcgggaata gaggccattc gggcgctgag tctattcggc ggccgggaag gtcggtgagc
     9781 cttgagtgga atacgggatt gggcgcgtat ccggaatcga tattgaatcg cttgtggagc
     9841 tctttaacga tgcctacagc aactactacg tcgacatcca ggttaacccg gctcgactac
     9901 acggactcat cgagagggag gatattaagg tcgacaactc cttcgtcgca tacgaggggc
     9961 agttacccgt gggtgtggtc ttcctaggcg tgagaggcag gcgcggctac atctgcggta
    10021 tgggtgtgcg cacgagccgc cagcggaggg gtatcggaga gctgctgatg cgacaggcat
    10081 tggccgaagc aagacgactg gggctcagtt cggtccagct ggaagtggtg gctgccaacc
    10141 gccgggcgat cgccctctat gccaaattgg ggtttgtgcc catgcgcaat ctggggctgt
    10201 gggagaggaa cacgccaccg ccgccggcgc cggcatcggc gcccaaatcc gcgcccacgc
    10261 ccgcacttgg tgcacgcgct actcgggcgt gccgggatga ggcccacgtt ccatcgcagt
    10321 ccggagtgcg cgtaggtgcg gtgcgcatgg caggcgtgct tcccctggta gatgcgttca
    10381 atgagatcag gccgtgctgg cagaatgagg cgcagtcgct cgtgaagatg agcgataggt
    10441 tcgtgggcta cgtctcccat ctcgacagcg cggccacagc gtatgtagtg ttcggcgact
    10501 cggcgagcgg cctgtacata gctgacttcg cagccgctcc caggctgggc aatgccgaga
    10561 ggcagagcat gtgtgagatt ctgttgtcgg cggtagaccg cgatgtgccc cgtgcatccg
    10621 cgaaggcctt caacatccct ctccagggct accaggaggc agttctgcgc gcccgcggat
    10681 tcgaccttac ccttatccag caggagatgg tgttgtgctt aaagtggcgc taactacatt
    10741 tgctacggtg ttcctggccg aactgggcga caagactcag cttgcgacca tgcttctggt
    10801 ggcgcagggc aagtcgcctc tcgcggtctt cctgggatca gcatcggctc tggtcgtgtc
    10861 gtcgctcgta ggtgtacttg caggtggtgt aatcgcaaag tacatccccg agatatgggt
    10921 gagagcggcg gcagggtcag gcttcatcat tctcggcgtg cttctgttgc tcggccggtt
    10981 ctgacataca caagcaggag gtgtgtccgg cacatgaaga ctaggatgct cgttctcgcg
    11041 gtgttggcgg cggtcatggc gatttcggct accctttccg tttgggcctt ccccgtatgc
    11101 gcggctggct tgcccagcct ggaggagagg atcaccgcgg ccgatcttca ctggtctcgg
    11161 cggtcgcagg cgggcgtgac tgaggcgctt ttgcagaacc tggaggatgc ggacagggac
    11221 taccctaacc agggtgaagt cctgtggagg ctctccaggg catactggtg gaagtcgact
    11281 cgcatcgcca aggacgacaa gaagactagg attgaatggt tggacaaggc caaggccaca
    11341 ggagagaggg cggccaaggc ggcgccggca aacccgaacg cccactactg gtacgcggct
    11401 gcgatggcgg agaccggtgt ggccaggggg atactgcaga gcctgtttat ggtgaagccg
    11461 acgagggaag cgctcgaacg cgggctggca gcagacccca atcacgccag ctgccattac
    11521 ctcatggcag agctgtatct acagctgcct gggcctccac tttcgatagg caacaagcag
    11581 aaggctgtgg aagaggcaag gctcgcagtc aaatacgggc ctgattcgtc atcccactgg
    11641 ctcgtgcttg ggcgcgcgct gatagccaca aagcaatacg gcgaggcgcg ccaggcgctg
    11701 ggcacgttgc tcgcgatggc gcctgaccag ggcgatccgg acggaacacg ggccgatcag
    11761 gacgaaggcc gggccgaatt gcagtctata gccaacaaat gaggtggatc cgacacgaag
    11821 gtagcttact gttcgcttgg gtgttccaag aacctggtag atacggaaac gatgatggga
    11881 ctcctgcgct cggccggcat ggatccggca gctgatcggg attcggctga cgtggtgttc
    11941 attaatacgt gtgccttcat tgccgactcg gcgcaggagt cggtcgacac gatactggag
    12001 ttcgcccgcg accggcgtga ggggagaata gatgcgctgg tggtggcagg atgcctggcg
    12061 cagcgttacg gccgttcgct gatggaggag atccccgaga tagacgctct gattggcacg
    12121 ggagactgcg gtctttgcgt ggaggccgtg catggtgcgc tcaggggcga gcgtccggta
    12181 cttgtgggca ctccgggttt cctggcagaa gagggaggcc cgcgagtcgt agctaccccg
    12241 gggcacttcg cgtacctcaa gatagcggaa ggatgcgcca acaggtgctc ttactgcctc
    12301 atcccgtctt tgcgcggccc tgcaaggagc agacccgtgg aggcaatcgt ctgcgaagcc
    12361 gagtcgcttg catcgtcggg cgtgcgggaa ctcatcgtga tcgcccagga cacgacggca
    12421 tatggacgcg atctgtacgg tcggccatcg ctggccaggc tcctacgcca gctggtaggg
    12481 gtagacggga tcgaatggat cagggtgatg tacacttacc cttctctcat agacgatgag
    12541 ctgattgagg tgtttgcggg cgaagagaga atcgtaaagt acctcgacat tccgatgcag
    12601 cacgggtcgg ataaggtcct caagatgatg aacagacgat catcgagggc tcagatgata
    12661 gacgtgtgcc ggcgcctgcg cgagcgagtg ccgggactgg tgatacgtac atcgcttatt
    12721 gtggggttcc ccggcgaaac agatgatgac ttcgaggaac tgctgagctt catcgatatc
    12781 gtgcgaccgg agcgcgcagg ggttttcgag ttctccgccg aggaagggac gcgtgcctat
    12841 gacatgcccg ggcaggtgcc cccggacgtt gcgtccagcc ggcggggggt cgcaatggag
    12901 gccctggctc gcatatcccg ccagttcggc gagtcgcgtg taggtagcgt cgtgcgggta
    12961 ctggtggacg ggccttcgga tgaatcggac ctcgtggtgg aagccaggtc gtatgccgag
    13021 gcgcccgagg tggacggcaa gatttacatt ggcgatgccg agctggccgt aggcgagatg
    13081 gcggacgtca gggtcaccga cgccggccag tacgacctgg tggcggagag ggcgtagcga
    13141 ggtttctgag gcatcgcctg agaacaggat tctgcgctct tcggcacctg agtgttcgac
    13201 gcaaccagtt attggctcgt cctattcgtt ttcgactagg ttttccgcgc aacctattcg
    13261 gattcttagc ggtttggtag gcacgagttg tctgaattcg cgccggcaac ggcgccacct
    13321 ttttctggag tcgcgcctgc ggcaaattcg caggcagagg cgtgttacat aatatggctg
    13381 tctttgtggg tttcccgacc cggtcgcgaa acgccctcaa acggctcact gtttgccagg
    13441 aacccccaaa ttcaggtggc ctagtcgctc tgagcgtcaa aacctctagc attctgagta
    13501 ggtacatcaa aaaacctatc acaattctca taggttcgcc gatcccctgc taaacagcgg
    13561 tttcaccaaa gaggctgcgt aaggcctcct tcgcttgtag ctggcgttgt cgagcccgac
    13621 ccggtcgccg gagaagtcct agcgcgatgg aaacaggggt ccaactgcga tatgcagtca
    13681 ggcccctgtt gacttccttg atgcttaacc gttcaggctc gcgccgttca agctcgcgct
    13741 gtgcagatca cgggaagatt cccctcatcc ggatagcctc ggcgaccttg gcgatggaga
    13801 ggatgtaggc gccggtgcgc atcgacagat ctcgctgctt ggcgaagtcc cacacctcgg
    13861 cgaagctgcg agtcatgatg ccgtcgagcc gggtattgac ctcttcctcg gtccagtaga
    13921 agttctggag gttctgcacc cattcgaaat aggagcagac gactccgccc gcgttggcca
    13981 ggatgtcggg caccacgata acgcccttct cctccaacat cttgtctgct tcggcggatg
    14041 tggggccgtt ggcgccttct acgatcaact tggccttgat cttgtcggcg ttcgcctcgg
    14101 tgatctggcc ctgcagcgca gccggtatga gaaggtctac atccagcgtg agcagttgct
    14161 cgttggagat gatgctcgct cccggcgctg tgtacccttc aataaacttg tgctccgcga
    14221 cgtacttgct gatctccggc acatccaggc cggccgggtt gtataccccg cctgtgacgt
    14281 cgctgacgcc gaccaccttg cagcctagct catgggcgag ctccgccgag acggacccca
    14341 cgttaccata tccctgaacc gcacaggtaa tctcagatgg cttctttccc agcctggcga
    14401 ggagctccct gacggcgaac acaacgcctc tgcccgtggc ctcgcggcga ccgagcgaac
    14461 cgccgatggg gatgggcttt ccagtgacca cgcccgggct aagaacgccg gtaagtacag
    14521 aatacgtgtc aacaaaccag cccattatct gcgcattggt gcccatgtca ggggcgggta
    14581 tgtccttttc agggcctatg atcggcagaa tggcatatac gtatcgacgt gtcagcctct
    14641 taagctcgtt ctgcgacagc ttcatcgggt caactcgtat gccgcccttg cctccgccgt
    14701 acgggatgcc tgctactgcg cacttccagg tcatccagga cgcaagcgcc ttcacctcgt
    14761 ccatgcacac gtccattgca taacggaccc cgcccttggc cgggccgcgc actgtggagt
    14821 gttggcatct gtaacccatg aacacttttg tgctgccgtc atccatctcc accggaagtg
    14881 cgacttccag tgtgcgttcg ggctcggcaa gtatttcgaa gatgcccggt tccagattga
    14941 gaatccgaga agcttcgcgg agcctggcga gtacctgctc atactggctt cctgccattt
    15001 tactacctcc aaacagtgta gtacatcgac aactaccaat tcgacacaca ggagtcggct
    15061 cctgccgggc gatgtaggtt cgtctcttcg aaaaggctcg ccgagagaga aatgttgacg
    15121 gatacgcagg aaggagttcg ccagcagaca gcgaagttgt atgcgtccaa caacctacat
    15181 ggggaggatg gttttatgaa gcgcactttg tcactgttgc tcgctgccgg gatgcttctg
    15241 gctctagcgc tgccggtatt ctcggctccc gctccgaagg tcgggatagt gttcgacatc
    15301 ggcgggcttg gcgaccagtc gtttaacgac gcagcataca gaggattgaa gaggctggaa
    15361 agtgaactag gcgccactac tacgtacatc gagtcccgca ccccgtccga ctacgagccc
    15421 aacctcgcca cgctggctag gcagggttac gacgttgttt ggggcatcgg ctttctgatg
    15481 gctgacgccg tcaagaaggt ctcgcagcaa ttcccgaata cgaggtttgg aatcatcgac
    15541 aatggctata gcgacgaaga atacaaggcc atgaagaatg tcctaggagt gctcttcaag
    15601 gagcaggagg gctcgttcct ggtgggcgtg ctcgcagccc ttactacgaa gaccaatatc
    15661 gtgggatttg tgggaggcat ggacttccct gtcatcgaga gatttgagtc ggggttcaag
    15721 gccggtgtgt ggaagacca
//
LOCUS       DAOTAC010000015        15684 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_3139_length_15684_cov_4.641052, whole genome shotgun sequence.
ACCESSION   DAOTAC010000015 DAOTAC010000000
VERSION     DAOTAC010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 15684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15684
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3139_length_15684_cov_4.641052"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..1289
                     /locus_tag="PLM74_01305"
     CDS             102..1289
                     /locus_tag="PLM74_01305"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HON41752.1"
                     /translation="MHTAAFANRMRDLGGDVVANMFALTEKADIISFAGGIPSPDALP
                     VERLHELADEILCTSGKQVLQYATIDGYGPLREWLADWMAETGVQATRENVLVTSGGQ
                     QGIDLAAKAFLNPGDCVLVERPTYLAAIPIFKTYEARFAAVDSDDEGIIPESFEEACR
                     RHNPKLLYVVPTFQNPTGVTIPADRRRAIAEIAGRYGVLVLEDDPYAKLRYDGDPAPA
                     IASFDDRGNSIFVGSFSKIVSPGLRMGYMVSPAHVQPRLAQCKQSTDFHTSNLSQRLI
                     YEFCARGYLGPHLEVIKKSYAEKRDAALRAMEKYFPDGVKWTHPQGGLFVWATLPEEI
                     DTTELIKHASERKVAFIPGVPFYFDETGHNTMRLNFSNASLDALDEGLHRLGTVIKEA
                     MDG"
     gene            1394..2947
                     /locus_tag="PLM74_01310"
     CDS             1394..2947
                     /locus_tag="PLM74_01310"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HON41753.1"
                     /translation="MALIEECLMRMPACAATQARWFRHKLSGASSCRLLDLSEPLPGS
                     GDPPVALAIVRFDGAGNYLIPVTLGSRHSGQAGRVLAHCDAPGGSTDMGEWNTSLIDA
                     ASDGAYVSAVLALIESNSDVRARHGVFEFRRIGAYSGWSVENVSSAYTNSVVIAHRSS
                     ALKTFRVLTAGINPDVEVGSALTQHAQFAEIARVQGEGTYVGTDGVRYSVAVQTEAIP
                     NVGDVWGAMGDALDGLWRRGAPKAGLPSDAPACSDLCSPHAIGRLGATVGRLHLALAS
                     IDLPGFGRSPVTPATFRGWRVRLVDRAAGAVARLDDALRRPGFLGDDALRLARLVMEA
                     WPGFESSLASGAWESDTADATNSDLGELIRCHGDLHLGQVLLRPDGRFAVIDFEGEPL
                     VPLSERRALESPARDVAGMLRSFSYAAHSARLRAASSGVHGHDLMLDWERQARLWFLQ
                     GYFGVVDGAGSRLWPAAHEARQSLISLFEIEKAFYEVDYEINNRPDWAMIPLSGILQV
                     LERWGGGRA"
     gene            2977..4110
                     /gene="glgA"
                     /locus_tag="PLM74_01315"
     CDS             2977..4110
                     /gene="glgA"
                     /locus_tag="PLM74_01315"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HON41754.1"
                     /translation="MSREYPPNVYGGAGVHVDYLSSSLADMMPVEVRCFGQREAIQNS
                     LAVRGYDPWLDLSRGSDPRLEKVLSPLSVDLALVKDPITSRVVHCHTWYTFFAGYLAK
                     MLYGARLVTTVHSLEPLRPWKEEQLGRGYECSSWMERIGIENSDRVIAVSQEMREDVL
                     RLFDVPPEKVVVVHNGIDLAGYRRTADSSALSSYGIKQPYVLFVGRISRQKGIMHLLD
                     AAASIQTGVSVVLCAGSPDTSEVEEELASRVASMDNVVWIDRMLPREHVIQLYSHAAV
                     FACPSVYEPFGIINLEAMACEIPVVASRVGGIPEVVVDGETGLLVPPADPAALAEAIN
                     LILGSEELYARFGRAGRARVEKHFSWQAIAMRTREVYESVVSE"
     gene            complement(4086..4472)
                     /locus_tag="PLM74_01320"
     CDS             complement(4086..4472)
                     /locus_tag="PLM74_01320"
                     /inference="COORDINATES: protein motif:HMM:NF024835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HON41755.1"
                     /translation="MSPVDAESACIGMRIRRARLARKMTQESLSEMCGVNASYIGQIE
                     RGEKQPSLRTLTAICRSLRVDLSTLLEADTSAPDAAVQELLEVVCGCPAEIVDAVTQH
                     ARIVVSTFQTLKPPDEPCVHSDTTDS"
     gene            4701..5135
                     /locus_tag="PLM74_01325"
     CDS             4701..5135
                     /locus_tag="PLM74_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41756.1"
                     /translation="MRSREDLHDILMECSEHLMWVDSMWSGAGDEERMEMCTMLAQIE
                     LELQQLIETRDVPWRVFRQATDLMAHASHSLNLLCYAEYTKKLSRLERQLRAATTDEG
                     WNAYLKIDTLKSDETALRAARQCPGCMCGHKRYDQFMTSKSS"
     gene            complement(5108..6169)
                     /locus_tag="PLM74_01330"
     CDS             complement(5108..6169)
                     /locus_tag="PLM74_01330"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HON41757.1"
                     /translation="MRVGFSGRPSSLSGAFAPSPRERIARCVSACSELGLGCLELPAG
                     VLFGKSGWRNQFLAACTEAPSSTGVAGRSRGGVDVLVRVRPSDCFGVRAPDHEGRDRT
                     EIGYARHLVRLVGGRLATVLMRSEHSIAVSDMSPPEGQGAARIGLVLSWSDQAAVLAA
                     ARAMRRSSQFVMLPLLSLGSEHGSRLERTVERALGVCRNENRGGDMYLFLSMPRSSRP
                     SANASTRDTGALLEAVRRFEEETASEIGVVFSGPGSEAHAHQAADIARELESPRLTGR
                     AGPPVPRLGSTVKIRDVELGLDMQYAIVSPEHASVMAGRLSSESPVARAIMGQPCGAR
                     VEVAAPDGRVVYELLEVMN"
     gene            6378..8309
                     /locus_tag="PLM74_01335"
     CDS             6378..8309
                     /locus_tag="PLM74_01335"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HON41758.1"
                     /translation="MCVMILSMCAFSAAAAPPQGPWVLRVEQGGAWADIPDAVIDLAG
                     QPFVSVRGLAQGIGASLSWIDVTREAVFTSDRTVAMLRAGDRQAYVNGQAVTLSAPPD
                     AESGRMAIAAPDIRALGLVAEIDGKSRVIKVSWPRSRIRSASVSAEHGVVAVVIEGDT
                     PFRYSDFVLRSPDRVVIDIYDAVLEPSVSTVDVADDIVKRVRAAMNRPGVVRVVVDLA
                     SALGYSVEYTAGAPARLAIKFQTRITDFSLDGEAPLPRLSVATTGRSAFSSPQLDADG
                     IMFIDFPSAQVCLDQAELVPESGPVKWARLAALEGGGARLRVKLEDGYSAIMRDRLCD
                     DNRVVVDFALTVCNVRSHEGPGCTVVDIATSGPVEFRPFRLTEPDRLVVDLPGVWAAG
                     SPAAEVHSRSVTQVRLAQFDEGTARAVLDLGEAWAHSWDVSPDGKGIRLTVASSPVFG
                     RTVVIDPGHGGTDPGAVKDGVYEKDINLDIALRLRQLLDSSGARVVMTREEDSSVQLS
                     ERSRMANEAMPDAIISIHCNSTTWDIFPSGTESYYFNSVPYSQELAALVHTCVVRELQ
                     LMDRKVRRGDYHMVRETRAPAVLVEVAFMSNGVDLQRLRDPESRQKAASGMFKGLSMY
                     LGSEMFRKWLAEAAGKVGL"
     gene            8351..9187
                     /gene="murI"
                     /locus_tag="PLM74_01340"
     CDS             8351..9187
                     /gene="murI"
                     /locus_tag="PLM74_01340"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017717724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HON41759.1"
                     /translation="MRYARIGILDSGVGGLTVARAIADAMPGAHLIYFGDTARMPYGD
                     RPPDQITRFAREIITFLLDQGVDAVAVACNTSSALALPAMQGDFDVPIVGMIECGAVA
                     AVEASRTGRIGVIATANTVGSGAYGRAITSLRPAAHVVQQACPLLVPMIERGEVSGEE
                     VRRVVRDYIQPLLAADVDSVVYGCTHYPFLDAEVRAATGPSIALVDPAIRVACAVKSA
                     VLARDPSLSGEPGVQTIPPRFITSGDPEVFAGLVHSLLGWRPGEVERISMDAKGLTSR
                     NA"
     gene            9180..9968
                     /locus_tag="PLM74_01345"
     CDS             9180..9968
                     /locus_tag="PLM74_01345"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HON41760.1"
                     /translation="MLELTVIGNQGPYAGPGGVCSGYLFSARSTAVLVDSGPGVMSGL
                     QRQIDFRGLSGIIISHYHPDHCSDFLTMRYAFHHANSCGQLPRPVPLLIPEPGWESFA
                     RDWVGQQYAELFDVDLIGDGRTVCLGDLRFEFRLMAHSVPSYAMLIREATAPGGGRAP
                     VAYTSDTGPCEALDSLAAQARTLLSEAGAGRAYSEGYGSPRPPSPGHLSPAEAGAIAR
                     AAGVDRLILTHLHPESRPREIEAAASEAMGASAEVAQQGRRYAL"
     gene            10036..10797
                     /gene="rph"
                     /locus_tag="PLM74_01350"
     CDS             10036..10797
                     /gene="rph"
                     /locus_tag="PLM74_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HON41761.1"
                     /translation="MRVDGRAPEELRTVQIARHYIKHAPGSVMVEMGDTRVLCAATYD
                     ERPPMHRRGSGQGWVTAEYSMLPCSTHERTPRERNTGRIGGRTQEIQRLIGRSLRSVM
                     RLADLGECTIWVDCDVIQADGGTRTASITGAFLAVVDLLASIDKWQGKPLPVSDFLSA
                     VSVGMVDGRLLLDLCYAEDSRAGVDMNVVMTAGGRLVEVQGTAEGEPFTRQDLADMLD
                     LAESGIRKLCDMQRNALGPLAERIGSGGENTGGGI"
     gene            10797..11435
                     /locus_tag="PLM74_01355"
     CDS             10797..11435
                     /locus_tag="PLM74_01355"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HON41762.1"
                     /translation="MSRQVVGSLVVATRNRGKLAEIRELLSRLDFTVKALDDYPGAPE
                     PEETGATFRENAIIKASAAASFTGEVAVADDSGLMVDALGGAPGVMSARYGGNGASDQ
                     EKYEKLLAEMKDVPDAERTARFVCVVAVASPDGSASSAQGVVEGSIARAPRGMGGFGY
                     DPVFLIPELGMTFGELPSATKNGMSHRARALAAAEPLILEALSQWNARHRRD"
     gene            11569..11645
                     /locus_tag="PLM74_01360"
     tRNA            11569..11645
                     /locus_tag="PLM74_01360"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11603..11605,aa:Pro,seq:tgg)
     gene            11969..12793
                     /locus_tag="PLM74_01365"
     CDS             11969..12793
                     /locus_tag="PLM74_01365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HON41763.1"
                     /translation="MNRRRFRRVRLDELLVQRGFFDDVKTAQGWIMAGSVIVDEVRED
                     KPGTMVDANAGVRVRGTDLPYASRGGLKLAAALDAFGVDVRGRTAIDAGASTGGFTDC
                     LLEHGAALVYSVDVGFGQLVGRLRQDPRVRNMERTNISDVTPGMLDPRPSLGVIDLSY
                     LSLQLSVPIVANLLDPEEESEIVALVKPLFEVASAVSDLTREQYRAAVWRALSAGTPA
                     GMAPRAVMASPVLGSRGTLEFLVWYTAGRGLMRDVASAQVDEAIGAGMDILSKAQG"
     gene            12809..13828
                     /gene="pheS"
                     /locus_tag="PLM74_01370"
     CDS             12809..13828
                     /gene="pheS"
                     /locus_tag="PLM74_01370"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008420799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HON41764.1"
                     /translation="MRDKVEALVERALSAIESAKSAEALEEVRVRILGRKGELTTLLR
                     QMKQVDPDDRPAFGAMVNAARDRLQQELDRRGQAMEVRERARRLESETIDVTMPGRVR
                     ASGAVHPLTQGIRDVTQIFVEMGFTVVEGPEIETDFYNFEALNTPQSHPARDVQDTFY
                     INDFVLLRTQTSPMQVRQMEKQDPPVRVVVPGKVYRRDPMDATHSPVFHQIEGLLVDK
                     DVTFADLAGTLTQFAQTFYGKDRKVRFRPHYFPFTEPSAEMDVSCALCGGKGCRVCKY
                     EGWIEILGSGMVHPNVLRAVGYDPDELTGFAFGMGVERIVMLKYGFDDVRLLYENDLR
                     FLAQF"
     gene            13856..>15684
                     /gene="pheT"
                     /locus_tag="PLM74_01375"
     CDS             13856..>15684
                     /gene="pheT"
                     /locus_tag="PLM74_01375"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HON41765.1"
                     /translation="MRVPLRWLKDYVDIELEPEQLADALTMSGTKVESIDRVGSGLRL
                     VTAGRAVSVTPHPESDRLRIVVLDIGPRQAPRRVVTAATNVTEGCVVPVVLAGGSLVD
                     GEEIGEAEFAGVKSEGMLLSARELGVSEDGSGILLLPDSVKPGDDIVDSLGLRDDVLE
                     LEITPNRPDCLCMVGVARELAAITRKGLRMPLTGCPEATQSARDLIKVVIEAPDLCRR
                     YCARVVRRVRSGQSPIWMQARLAAAGVRPISAVVDVTNYVMLELNQPLHAFDYNRVAQ
                     HTIVVRRAQAHERLTTLDGAERELNTDMLLIADPKRALAVAGVMGGLASEITESTDTV
                     LLESACFARQSVWRTSRTLKLRTEASSRFEKGLDPAMAPPALDRAASLLSQMGAGEAC
                     SGIVDAYPSPEAPVVVETTDRWIEHRLGSGIGADGIADCLARLGFGVGVRASQDGATK
                     MTVSIPSSRGDVREPIDLVEEVARIWGYNEVPATIPEGTLPGGYSEEYAFFEQVKQAL
                     AGAGGSEALTFPFVSEADFDVMGFAPEDPRREAIRVANPLVEEQSLMRTTMYPAMLAA
                     LRTNINRRRRGVFLFEIGRTYTPTDAVRAGLEPSRGETPAVERP"
BASE COUNT         3070 a         4386 c         5107 g         3121 t
ORIGIN      
        1 gccgctgtgc agaaagcagg taagtgaggc aggccaaaga atggtctaga tgtggcacac
       61 agtgaaacac agtcgagcat tctgaaggga gtgacggtcg attgcatacc gccgcattcg
      121 cgaacaggat gcgggacctc ggcggggatg ttgttgccaa catgttcgcc cttaccgaga
      181 aggccgacat catctcgttc gcaggaggca taccctcgcc cgacgcgctg cctgtggagc
      241 gcctccatga actggcagat gagattctgt gcacctctgg caagcaagtc cttcagtacg
      301 cgaccattga cggctacggc cctctgcgag agtggctggc agactggatg gcggagactg
      361 gtgttcaagc aacgcgcgaa aacgtgctgg ttacttcagg gggccagcaa ggcatcgatc
      421 tggctgcgaa ggctttcctg aaccccggcg attgtgtact cgtggaacga cccacttacc
      481 tggctgcgat tcccatattc aagacgtatg aagccaggtt tgcagctgtg gattccgacg
      541 acgaaggcat catacccgaa tccttcgaag aggcctgccg caggcacaat ccgaagcttc
      601 tgtatgttgt tcctacattc cagaatccaa caggcgtaac catccccgcc gaccgcagac
      661 gcgccattgc ggagattgcg gggaggtacg gagtgctggt actggaagac gatccatacg
      721 ccaaactcag gtatgacggt gatccggcgc cggccatagc gagcttcgat gatcgaggca
      781 actccatctt cgtgggcagc ttctcgaaga tagtctcgcc aggcctgcgc atgggataca
      841 tggtgtcgcc cgctcacgta cagcccaggc tggcacagtg taagcagagt actgacttcc
      901 atacttccaa cctctcgcag aggctgatat acgagttctg cgcgcgtggg tatctcggcc
      961 ctcacctaga ggtcataaag aagtcctatg cggagaagcg agatgcagct ctgcgcgcca
     1021 tggaaaagta cttccccgat ggagtgaagt ggacacatcc acagggtggg ctgttcgtgt
     1081 gggcgacact gcccgaagag atcgacacca ctgagctgat caaacatgcg tcggagagga
     1141 aggtcgcatt catacccggc gttccgttct actttgacga aaccggacat aataccatga
     1201 ggctcaattt cagcaacgca agccttgatg ctctggacga gggattgcac cgtctgggta
     1261 ctgtcatcaa ggaagctatg gacgggtaga cgccgcgtca ggcgtgttgt gagcgtgtgt
     1321 tcggcccggg cgctcctttt cccggggatg cgtccgggtt ccgtctattc tggaggaggt
     1381 gggtcaggtg tcgatggcgc tgatagagga gtgtttgatg cgaatgcctg cctgcgcagc
     1441 gacacaggcc cgatggtttc gccacaagct gtcaggcgcc tccagctgca ggttgcttga
     1501 tctctccgaa cctttgcccg gatcgggcga tccacctgtg gcgcttgcaa tcgtcaggtt
     1561 cgatggagcg ggcaactacc tgatccccgt tactctgggc agcagacact cgggccaggc
     1621 ggggcgggtt ctggcgcact gtgatgcgcc gggcggaagt acggatatgg gcgagtggaa
     1681 cacaagcctg atcgatgcag catcggacgg agcttacgtg agcgctgtct tggcgctgat
     1741 tgagtccaac agtgacgtta gggcgcgaca cggggtattc gagtttcgca ggattggcgc
     1801 atatagcggt tggtctgtgg agaacgtgag cagtgcctac actaacagcg tcgtgattgc
     1861 ccacaggagt tccgcgttaa agaccttcag ggtcctgacg gcgggcatca atcccgacgt
     1921 ggaagtcggg tctgccctga ctcagcacgc ccagttcgcc gagatcgccc gtgtgcaggg
     1981 agaaggcacg tatgtgggaa cggatggcgt gagatattcc gtggcggtcc agaccgaggc
     2041 catcccaaac gtgggcgacg tgtggggggc catgggcgac gcgctcgacg gcctctggcg
     2101 ccgcggagcg cccaaggccg gcctgccgtc tgacgcgccg gcttgctccg acttgtgctc
     2161 gccccatgca attggacgcc ttggggcaac cgttggacgt cttcacctgg ccctggcgtc
     2221 gattgatctg cctggtttcg gcaggtctcc ggtcacccct gccacgttta ggggatggcg
     2281 ggttcgcttg gtggatcggg ccgcaggcgc agttgcccgc ctagacgacg cccttcgtcg
     2341 cccgggtttt ctcggcgacg acgctctcag gttggcacgg cttgtcatgg aggcatggcc
     2401 gggttttgag tccagccttg cctctggggc gtgggagagc gatactgccg atgctactaa
     2461 cagtgatctc ggcgagctga ttcggtgcca cggagatctc catcttgggc aggttctcct
     2521 caggccggat gggcgattcg ccgtcattga ctttgaaggt gagccgctgg ttcctctctc
     2581 agagcggcgg gcgctggaaa gccctgccag ggatgtggcc ggcatgttgc gctcgtttag
     2641 ttacgctgcg cattcggcga ggctacgtgc cgcctcctcg ggcgtacacg ggcatgatct
     2701 gatgctcgat tgggaacgcc aggcgaggct gtggtttctg cagggctatt tcggcgtagt
     2761 agacggcgcg ggcagtcgcc tgtggccggc ggcgcatgag gcgagacagt cgctgatatc
     2821 cctctttgag atcgaaaagg ctttctatga ggttgactat gagatcaaca acaggccgga
     2881 ctgggcaatg attcccctat cgggaatcct gcaggtcctg gaaagatggg gaggtggccg
     2941 agcttgacgg cgaaacagcc tttgagtgtc actatcatga gtcgcgaata tccgcctaac
     3001 gtgtacggcg gtgccggagt gcacgtggac tacctgtcgt ctagccttgc cgacatgatg
     3061 ccggtcgagg tcaggtgctt cggccaaagg gaggcgatcc agaactcgct ggctgtcaga
     3121 ggatacgacc cgtggttgga tctgtcccgg ggttcagatc ctcgcctcga gaaggtgctg
     3181 agcccactct cagtcgatct ggccctcgtg aaagacccga tcacctccag agtggtacac
     3241 tgtcacactt ggtacacgtt cttcgccggc taccttgcca agatgctgta cggcgctagg
     3301 ctcgtcacta ccgtgcactc tctcgaacca ctcaggccgt ggaaggagga acagctgggc
     3361 cgaggctacg agtgcagttc ctggatggag aggatcggca tcgagaactc ggaccgggta
     3421 atagccgtct ctcaagagat gagagaagac gtgctcaggt tgttcgacgt cccgcctgag
     3481 aaggtagtgg tggttcataa cggaatagac cttgcggggt atcggcgcac cgccgactcg
     3541 tctgcactgt caagctatgg catcaagcag ccgtatgtgc ttttcgtggg cagaatctcc
     3601 cggcagaaag ggataatgca cctactggac gcggcagcct cgattcagac gggtgtgagc
     3661 gttgtgctgt gtgcaggaag ccccgatacc agtgaggtcg aggaggaact ggcttcccgc
     3721 gtggcctcga tggacaatgt ggtctggatc gacagaatgc tcccaagaga gcacgtcata
     3781 cagctgtaca gccatgctgc ggtattcgcc tgtccgtctg tgtacgagcc cttcggcata
     3841 ataaacctgg aagccatggc gtgcgagata cccgtggtgg catcccgggt aggaggtatt
     3901 cccgaagtag tggtagacgg cgagacgggg ctactagttc cacctgcaga ccctgccgcc
     3961 ctggcagagg cgatcaacct cattctgggc tcagaagaac tatatgcccg gtttggacgg
     4021 gccgggcgcg cccgcgtcga aaagcacttc tcatggcagg cgatagcgat gcggacccgg
     4081 gaggtctacg aatctgtggt atcggagtga acacaaggct cgtcgggcgg cttgagtgtt
     4141 tggaatgttg acacaacaat tcgggcatgt tgcgtcaccg cgtccacgat ctcagcaggg
     4201 caaccgcaca caacctctag caattcctga acagctgcgt caggagcact cgtgtcggcc
     4261 tcaagcagcg tagacagatc cacgcggagc gacctgcaga tggcggtgag cgttcgcagc
     4321 gatggttgct tttcgccgcg ctcgatctgc cctatgtagc ttgcgttcac gccgcacatc
     4381 tccgagagcg attcttgtgt catcttgcgt gcgagccgcg ctctgcgtat tcgcatgcct
     4441 atgcaggccg attccgcgtc cactgggctc accttcctca acagcaatag tacccctgaa
     4501 cgagcaggca ttgaatatac cgggagcact acttgaatgt cgtattatgt gctcggagta
     4561 ttgatacgca gcttaacacg gccaatgccc ggatactacg acccgcaaga taccgcccga
     4621 ggttatcatc cggacgggcg atactcattc ggcagatgct gtgctaggat gtggaagacg
     4681 aggtgatggg aggcgacgag gtgcgcagtc gcgaggatct gcacgacata ctcatggagt
     4741 gctccgaaca ccttatgtgg gtagacagca tgtggtcggg ggcaggcgac gaggaacgaa
     4801 tggagatgtg tactatgctg gcacaaatcg agctcgagct tcagcaactg attgagacga
     4861 gggacgtacc atggcgcgtc ttcaggcagg caacggacct aatggcgcac gcctcgcaca
     4921 gcctcaacct gctctgctat gccgagtaca caaagaaact gtcgcgccta gagcggcagc
     4981 tcagggcagc aacaacagat gagggatgga atgcttacct caagatcgac accctaaagt
     5041 cggatgaaac tgcactccgg gccgcccggc agtgtccggg atgcatgtgt ggtcacaagc
     5101 ggtacgatca gttcatgacc tccaaaagct cgtagacaac tctcccatca ggcgcggcta
     5161 cttccacccg tgcaccgcat ggttgcccca tgatcgctct ggccaccggc gattcgctcg
     5221 acagtcggcc cgccatgacc gaagcgtgct ctggcgagac tatggcatac tgcatatcga
     5281 gccccaattc cacatcgcgt atcttgaccg tcgaaccaag cctgggcacc ggcggccccg
     5341 ccctgccggt gagtcggggg ctttcgagct cacgcgcgat gtcggcagct tgatgggcat
     5401 gcgcctcgct gcctgggccg gaaaaaacca ctccgatttc gctcgctgtc tcttcttcga
     5461 atctgcgtac tgcctcgagc agcgccccgg tgtcacgtgt cgaagcattg gctgagggac
     5521 gtgaggagcg aggcatggat aggaacaggt acatgtctcc tccccggttt tcgttccggc
     5581 acactcctag cgcgcgttca acggtccgct cgagcctgga gccatgctcc gacccaaggc
     5641 tcaacaacgg gagcatgacg aactgcgatg agcggcgcat tgctcgtgcg gcagccaaga
     5701 cggcggcttg gtccgaccag ctcagaacta ggccgatgcg agctgctccc tgtccttcgg
     5761 gcggactcat atcggacact gcgatgctgt gttccgacct catcagcact gtcgcaagcc
     5821 tacctccgac cagcctgacc aggtggcgtg cgtaaccaat ttcggtccgg tcccgccctt
     5881 cgtgatctgg ggcacgaaca ccgaagcaat ctgacggtct cacccgcacg agcacgtcca
     5941 cgcctcccct ggaccggccc gccacaccag ttgaagaagg tgcttcggtg caggccgcca
     6001 ggaactgatt gcgccatccg ctcttgccga aaagcacccc ggcaggcaac tccaggcatc
     6061 cgaggccaag ttcagagcag gcagacacgc atcgagctat gcgctcccgg ggagacggcg
     6121 cgaaagcccc cgaaagcgac gaaggtctgc ccgaaaaacc cactcgcatg cgctcacctc
     6181 caggcaactg gtgctaggtt tcccagaaac gccacgcaat agtcgcgccg tgggtgtttc
     6241 aacgagaagc atcaaatggc acatggcctg accaggtctg tacaaaggaa aagcgtgtag
     6301 aaagtcgaaa agaagcagat agacccgaaa gccagggagg gggcaggcgt ttgcgttgta
     6361 cggggatggc gttttcaatg tgcgtaatga ttctgagcat gtgtgcgttc agcgccgctg
     6421 ccgcgccgcc gcagggaccc tgggttctcc gtgttgagca gggcggggct tgggcagata
     6481 tccccgatgc ggtcatagac cttgcgggtc agcctttcgt atctgtgagg ggcctagctc
     6541 agggtattgg ggccagcctg agctggattg acgtgacccg ggaggctgtg ttcacatccg
     6601 accgcacggt agccatgctg cgagcaggcg atcggcaggc ctacgtgaac ggccaggctg
     6661 tgaccttgtc ggcgccgcct gacgccgagt caggccgcat ggccattgcc gcccccgata
     6721 ttcgcgccct gggcctcgtg gcggagatcg acgggaagtc tagggtcatt aaggtgtcat
     6781 ggccccgcag ccgaattagg agcgcttcag tgtcggcgga gcatggcgta gttgccgtcg
     6841 tgatagaggg cgatactcca tttagatact ctgacttcgt gctgcgctca cccgaccgcg
     6901 tggtaataga catatacgac gcagtgctcg agccaagcgt gtctacggtg gatgtcgctg
     6961 atgacatcgt caaacgcgtt cgcgccgcca tgaaccggcc cggggtggtg cgagtcgtgg
     7021 tcgacctcgc ttctgcgctg gggtattcgg tcgagtacac ggccggcgcg ccggcccggc
     7081 tggcgatcaa gttccagaca cggataaccg acttctcgct ggacggcgaa gcacccttgc
     7141 ctcggctttc ggtggcaacc accggccgtt ctgcgttcag ttcaccgcag ttggatgccg
     7201 atgggatcat gtttatagat ttcccctctg cccaggtctg cctcgaccag gcggagcttg
     7261 tgccggaaag cggacctgtg aaatgggcca ggctcgccgc gctggaaggt gggggtgccc
     7321 ggttacgtgt caagcttgaa gatgggtatt cggcaataat gcgcgaccgg ctgtgcgatg
     7381 ataacagggt agtagtcgac tttgcgctca cagtgtgcaa cgtgcgatcg catgagggcc
     7441 cggggtgcac cgttgtcgac atcgcgacat ccggccctgt ggagttccgg ccatttaggt
     7501 tgaccgagcc ggaccgcctg gtcgtagatc ttcctggagt ctgggcggcc ggcagccctg
     7561 cagcggaggt gcattcgcgc tcggtgaccc aggtcaggct ggcgcaattc gatgaaggta
     7621 ctgcgagagc ggtgcttgac ctcggcgagg catgggcgca ttcgtgggac gtttcgcctg
     7681 acggcaaagg gatcaggctg acagttgctt cgtcccccgt gttcggtcgg actgtcgtga
     7741 tcgatccggg tcacggcgga acggatccgg gagcagtgaa ggacggagtg tacgaaaagg
     7801 atataaacct ggacatcgca ttgaggctca ggcaattgtt ggattcgtcc ggagccagag
     7861 tggtcatgac acgagaggaa gactccagtg tacagctgtc cgaaaggtcc aggatggcta
     7921 acgaagccat gcccgacgcc attatctcga tccactgcaa ctcaacgacg tgggacatat
     7981 tcccaagcgg cactgagtcg tattacttca actcagtgcc ttacagccag gagcttgccg
     8041 ccttggttca tacatgcgta gtgcgggaac tgcagctgat ggatagaaag gtgcgccggg
     8101 gcgactacca catggtgcgc gaaacgcggg cccctgctgt cctggttgaa gtggctttca
     8161 tgagcaacgg tgtggacctg cagaggttgc gcgatcctga gtcccgccag aaggcggcat
     8221 caggcatgtt caaggggctc tcgatgtacc ttggcagcga gatgttccgc aagtggcttg
     8281 cagaggcagc cgggaaagtc ggcctataag ctcttgacct attcggctcg gcagagagta
     8341 taatagtcac atgcgatacg caaggatagg catactggat tccggggtag gcgggctcac
     8401 tgtggcgcgt gcgatcgctg atgcgatgcc cggcgcccat ctcatatact tcggcgatac
     8461 ggcccggatg ccgtacggag ataggccgcc ggatcagatc actcgattcg cgcgcgagat
     8521 catcactttt ctactggacc agggcgtgga cgccgtggcg gtggcgtgca ataccagctc
     8581 tgccctggct ctacctgcga tgcagggcga cttcgatgtg cccattgtgg gcatgatcga
     8641 atgcggggcg gtagcggcgg tcgaagcatc ccgcacaggg cggatcggcg tgattgccac
     8701 ggcaaacact gtagggagcg gcgcatacgg gagggccata acatcgttgc gcccggcggc
     8761 tcacgtagtg cagcaggcat gtccgttgct ggttcccatg atcgaacgag gagaggtatc
     8821 aggcgaggaa gtcaggcgcg tcgtgcgcga ttacatccaa cctctgctgg ctgcagacgt
     8881 cgactcagtc gtgtatggct gcactcacta tccgttcctt gatgccgagg tccgcgcggc
     8941 tacggggccc tcgattgccc tggtcgatcc ggcgattcgg gtggcctgtg ccgtgaagtc
     9001 cgccgtgctg gcgcgcgacc catcgctttc aggggagccg ggtgtgcaga cgattcctcc
     9061 gcgcttcatt accagcggag atcccgaggt cttcgccggg ctagttcact cacttctggg
     9121 ctggcgcccg ggcgaagtgg agagaatcag catggacgca aaggggctga cgagccgcaa
     9181 tgcttgagct caccgtgatt ggcaaccagg gcccatacgc cggcccgggc ggcgtatgct
     9241 ccggctatct gttctctgcc agatccactg ccgtactcgt cgactcgggc cccggagtca
     9301 tgtccggctt gcagcgtcag atcgactttc gcggcctgag cggcatcatc attagtcact
     9361 accaccccga ccactgttcg gattttctta ctatgcgcta tgcttttcac catgccaact
     9421 cttgtgggca gctgcctcga cctgttccgc tccttatccc cgagcctggc tgggagagct
     9481 tcgcccgcga ctgggtgggg cagcagtacg ctgagctttt cgatgtggac cttatcggcg
     9541 acggccgcac cgtgtgcctg ggcgatctcc ggtttgagtt caggctcatg gcgcactcgg
     9601 ttccttcgta cgccatgctc atcagggaag caactgctcc agggggcggc agggcccctg
     9661 tggcctacac atcggatact ggtccgtgcg aagcgttgga tagccttgca gcccaggcac
     9721 gcaccctcct ttcggaagcg ggtgcgggcc gagcctattc ggaggggtat gggtcgccgc
     9781 gccccccttc gcctgggcac ttatcgcctg ccgaggccgg tgcgattgca cgggcagccg
     9841 gcgtggacag gcttatcctc actcatttgc atcccgagag tcggccccgg gagatagagg
     9901 cagctgcgag cgaagccatg ggcgccagcg ccgaggtcgc acagcagggc cgccgatacg
     9961 cgctgtagcg ctgatacgcg ctgtagcgcc gatacgtgtt ctagctctga acatattgag
    10021 gagtggagag acgatatgcg agtagatgga agggctcccg aggagctcag aacggttcag
    10081 atcgcaaggc attacataaa gcatgccccc ggatcggtca tggtggagat gggagacacc
    10141 cgagtactgt gtgccgcaac atatgatgaa cgtcctccga tgcatcgcag gggcagcggg
    10201 caaggctggg ttactgcgga gtactccatg cttccatgct ctactcacga gcgcactccg
    10261 cgggagcgaa acaccggcag aatcggaggg cgcacacagg agattcagag actcatcggt
    10321 aggtcgctca ggtcagtgat gcgccttgcc gatctgggcg agtgcaccat atgggttgat
    10381 tgcgacgtga tccaggccga cggaggcacc aggactgctt cgataactgg tgcttttcta
    10441 gctgtggtgg atcttctggc atccatagac aagtggcagg gaaagccgct gccagtgtcg
    10501 gactttctgt cggccgtcag cgtaggtatg gtcgacgggc gactcctgct tgatctgtgc
    10561 tacgcagaag actcgcgcgc cggcgtggac atgaacgtag tcatgacggc tggcggtcgc
    10621 ctcgtcgaag tccagggtac ggcggaaggc gagcccttca cgaggcagga ccttgccgac
    10681 atgctcgatt tggccgagtc aggcatccgc aagctgtgtg atatgcagcg aaacgcactt
    10741 gggccgttgg ccgagaggat tggttccggc ggggagaaca ccggcggggg catatgatgt
    10801 cacgccaagt tgttggcagc ttagttgttg ccaccaggaa ccggggaaaa cttgctgaga
    10861 tccgtgagct gctctcgcga cttgacttca ccgtaaaggc tttggatgac taccccggcg
    10921 cgcccgagcc tgaagagacc ggggctactt tccgcgagaa cgccatcatt aaggctagcg
    10981 cagccgcgtc cttcacaggc gaggtggcgg tagccgatga ctcaggcctg atggtagatg
    11041 cccttggagg agcgcctgga gtgatgtcgg cgagatatgg gggcaatggc gcttccgacc
    11101 aggagaaata tgagaaactc cttgctgaaa tgaaggacgt cccagatgcc gaaaggacgg
    11161 caaggttcgt atgcgttgtg gctgtggcct cgccggacgg gtccgcgagt tccgcccagg
    11221 gcgttgtcga ggggtcaatc gcccgtgccc cgcgcggaat gggcggattc gggtacgatc
    11281 cggtgtttct cattcccgag ctcggcatga cattcggtga gctgccgtcc gccacgaaga
    11341 acggcatgtc acatagggcg cgggcgcttg cagccgctga acccctcatc ctcgaggccc
    11401 tatcccagtg gaacgcgcgc cacagacggg actaggattt cgccaggcgc cacacccgtc
    11461 gatgggatgt gcggtccttg ctatccgccc gcgatatgcc ggccgtccga gaggggcgat
    11521 gtcggggcgt tgactggcct ctggggctat ggtagactcg ggatgtgccg gggcgtagct
    11581 cagtttggca gagcgcatgg tttgggacca tgatgtcgca ggttcaagtc ctgtcgcccc
    11641 gaccattagg ttgaaactta atatggcccc aatggtgatg atggagagta gtaaggccgg
    11701 caaccgttcg gtcagggagg gtgcgccgcg actgaaagcg ccccgggtcg gtccggcatg
    11761 aattcgctcc tgaactgcac actgaaagca gagcccacac aagcggcgcg cgagtaggcg
    11821 gagccgggcg cctcccgtta caggggcagg gcatcatgtg tgcccgggaa agaggcctag
    11881 cggcgaatat gggtggtacc acgtagtctg cgacttgcgt cccatgcgca attccgcagg
    11941 ctttcttgtt ttggaggcgg aatctactat gaatcgcagg cggttccggc gcgtccgtct
    12001 tgatgaactg ctggtgcaac gaggtttctt tgacgatgtg aagacggccc agggatggat
    12061 catggcggga agcgtcatag tggacgaggt gcgagaagat aagcccggaa ccatggtgga
    12121 cgccaatgcc ggcgttcgcg taaggggtac ggaccttccg tacgccagcc ggggcgggct
    12181 gaagctggca gctgccctgg acgcattcgg agtggacgtt cgcggccgaa cggcgatcga
    12241 tgccggcgcc tcgaccggcg ggttcactga ctgcttgctc gaacacggcg ctgccctagt
    12301 ctactccgtg gatgtcggat ttgggcagct tgtgggtagg cttaggcagg atccacgtgt
    12361 tcgcaacatg gagcggacta acatcagcga cgtgacacct ggcatgcttg atccccggcc
    12421 gtcactgggc gttatcgatc tttcgtatct gtcgctgcaa ctatccgtgc ccatagtggc
    12481 gaaccttctc gatccagagg aggagtctga gattgttgcg ctggtgaaac cgcttttcga
    12541 ggttgcatcg gccgtttccg acctgacccg ggagcagtat cgcgcggccg tgtggcgggc
    12601 actctcggcc ggcactccgg cggggatggc gccccgagct gtcatggcct cgcctgtgct
    12661 ggggtcacgg gggacgctgg agtttctcgt ttggtacacg gcagggcggg gactgatgcg
    12721 cgatgtcgcg tcggcacagg tagacgaagc gataggagct ggcatggata ttctgagcaa
    12781 ggcacaagga tgacaggagt ggtgcagcat gcgagataaa gtcgaggcct tggtggagcg
    12841 tgcgctgtcg gcgatagagt cggcgaagtc cgccgaggcg ctggaagagg tgcgcgtaag
    12901 gatccttgga aggaaaggcg agttgactac cttgctgagg cagatgaagc aggtcgaccc
    12961 tgacgatcgc ccggcatttg gcgccatggt aaacgcagca cgagatcgac ttcagcagga
    13021 actcgatcgc aggggccagg caatggaagt ccgggaacgg gctcggcgac tcgaatccga
    13081 gaccatcgat gtgaccatgc cgggcagagt acgtgcatct ggtgctgtgc atcccctaac
    13141 tcagggcata cgagacgtca cgcagatctt cgtcgagatg ggtttcactg ttgtcgaggg
    13201 gccggagatc gagacggatt tttacaactt cgaagctctg aacacgccgc agtctcaccc
    13261 tgcccgagat gtgcaggaca ctttctacat caatgatttc gtgttgcttc gcacgcagac
    13321 atcgcccatg caggtcaggc agatggagaa gcaggacccg cccgttcgtg tggtagtccc
    13381 ggggaaggtg taccggcgcg atccgatgga cgcaactcac tcgcctgtgt ttcatcagat
    13441 tgaggggctg ttggtcgaca aggacgtgac ctttgccgac cttgcgggaa cgcttaccca
    13501 attcgctcag accttctacg gaaaggatag gaaagtgagg ttccgacctc actacttccc
    13561 gttcacagag ccctcagccg agatggatgt atcatgcgct ctgtgcgggg ggaagggatg
    13621 tcgggtgtgc aagtacgagg gatggataga gatactcggg tcaggcatgg tgcatcccaa
    13681 tgtgctcagg gccgtaggct acgatccgga cgaactgacg ggtttcgcat ttggaatggg
    13741 cgtagagcgg atagtgatgc tcaagtatgg tttcgatgat gtgcggcttc tgtacgaaaa
    13801 cgatcttcgg tttctggcgc agttctgatg aggccgacga aagaggtgct ggcgaatgcg
    13861 tgttccacta cgatggctga aagactatgt tgatatagag cttgaacctg agcaactggc
    13921 ggacgctctc accatgagcg ggactaaggt ggaaagcatc gacagagtgg gcagtggact
    13981 gaggctcgtc acggcgggac gcgccgtgtc ggttactccc catcccgagt ccgaccgcct
    14041 gcgaattgtt gtcctggata tcggcccccg ccaggcgccg cggcgcgtgg tgacggccgc
    14101 aacgaatgtg accgaaggct gtgtcgtacc tgtggtgctg gctggagggt cgctggtcga
    14161 tggggaagag atcggggagg cggagttcgc gggcgtcaag tccgagggca tgctcctttc
    14221 cgccagggaa ctgggcgttt cggaggacgg ctcggggatc ctgcttctgc cggactcggt
    14281 gaaaccaggc gatgacatag tggattcgct ggggctgcgc gatgacgttc tggagctcga
    14341 gataactccg aatcgtccgg attgcctgtg catggtcggg gtcgcccgcg aactagctgc
    14401 cataacccgt aagggactgc gcatgcccct tacggggtgc cccgaagcga ctcaatccgc
    14461 tcgcgatctg atcaaggtag tgattgaggc gcctgatctg tgccgccggt actgcgcaag
    14521 ggtggtccgg cgcgtgcggt ccgggcagtc gcccatatgg atgcaggcgc gtcttgcggc
    14581 tgcgggcgtc agacccatca gcgccgtggt agatgtgacc aactacgtga tgctcgaact
    14641 caaccaaccc ctccacgcct tcgattacaa cagggtagcc cagcatacaa tcgtcgtgag
    14701 gcgcgcccaa gcccatgagc ggctgaccac gctcgatggg gctgagcggg agctcaacac
    14761 cgacatgctc ctaatcgccg accccaagag agcactggct gtagcaggcg tcatgggcgg
    14821 actggcatcc gaaataaccg aaagcaccga cacggtgctc ctcgaatccg cttgtttcgc
    14881 gcgacagagc gtgtggcgca cttcccgaac cctgaagctg cgcactgagg catcttcacg
    14941 attcgagaag gggcttgatc cggccatggc gccgcccgcg ctggaccgcg ccgcgagcct
    15001 gctctcccag atgggagcgg gtgaggcttg cagcgggatt gtcgatgcgt acccatcgcc
    15061 ggaggcgccg gtggtcgtag agaccacgga tcgatggatt gagcatcgcc tgggctcggg
    15121 gattggggcg gacggcattg cggattgcct cgccaggcta ggctttggtg tgggcgttcg
    15181 ggctagccag gatggggcga cgaagatgac ggtgagtatc ccttcttcca gaggagatgt
    15241 tcgggaacct atagatctcg tggaggaggt cgctcgtatc tgggggtaca acgaggtgcc
    15301 tgccaccatc cccgagggta ctctgccggg cggctactca gaggagtatg cctttttcga
    15361 acaggtgaag caggctctgg caggcgcagg aggcagtgag gctctcacgt ttccattcgt
    15421 atccgaggca gatttcgacg tgatggggtt tgcgcccgag gaccctaggc gtgaggcgat
    15481 acgagtagct aatccactgg tggaagagca gtcgctaatg cgcactacga tgtatccagc
    15541 gatgctggct gcgctgagaa ccaacatcaa caggagaaga cgcggggtct tcctcttcga
    15601 aataggcaga acgtacaccc ccactgatgc ggtcagggcg gggctcgaac cctcccgcgg
    15661 cgagacgccg gcagtggaaa gacc
//
LOCUS       DAOTAC010000016        15247 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_3263_length_15247_cov_4.313981, whole genome shotgun sequence.
ACCESSION   DAOTAC010000016 DAOTAC010000000
VERSION     DAOTAC010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 15247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15247
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3263_length_15247_cov_4.313981"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="PLM74_01380"
     CDS             complement(<1..658)
                     /locus_tag="PLM74_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41766.1"
                     /translation="MSVARMRVKTKPGAARAGRRWGRTVAMLVCLLAAASVTGCRDAR
                     EIESVGFVLGLGVDETADGRIEVWAQVALPSAKPAEGEKQESWTVRSVGDTVWDAVRQ
                     LNGRSTKVLFRAHVRALVFGERFARGGVARALDALARDREFRYKSWVFVTSDPVGDVL
                     GAQTEQSSSAALFLDDVMRNTARSLTAPRSRFLDLLTSIEQPGDQPLLARVRLVESED
                     G"
     gene            complement(633..1799)
                     /locus_tag="PLM74_01385"
     CDS             complement(633..1799)
                     /locus_tag="PLM74_01385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endospore germination permease"
                     /protein_id="HON41767.1"
                     /translation="MKTARTAETKGTAETAGDRPKDEIYYTQIVVITAMIVFATGFLA
                     AARILVPVAGTAAWISALIGGVIGLGIVVLAGYLAARFPDHSLVRMAERIFGAPLGKL
                     IGLTFAAYCTVVLGLGSRESVLAIWIAFLQTTPPIIISGVFYALIIYAAYLGIEAIAR
                     VSLIFFTLVVISLLVLTALAVPVVVPGRLLPVVGNGLRPLIHGGFLAASYAGESVVVL
                     AFIRHLKDKRSASRAMATGVAAGMVVMATATGFGVMMLGVRGISRVTFPVLETARMVG
                     FGQFLERAEILFLALWFSAALLKLAAVFYASITSVADVLGLRDYRPLIIPYALLGTAL
                     SLIPKTVLETFDYLSMFVKYSVWYAIALPVVLLVGAVVRGSRRGGENVRGKDAR"
     gene            complement(1744..3507)
                     /locus_tag="PLM74_01390"
     CDS             complement(1744..3507)
                     /locus_tag="PLM74_01390"
                     /inference="COORDINATES: protein motif:HMM:NF015292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HON41768.1"
                     /translation="MRFLRRLARGGMRRPRMMLGRPLLPLEEVADGIGTAGPEGEDAG
                     TPRDSDAALPEPPIPDTLEGVERRLRDLMGEGSPDVIIRRLIIWTNPEIEGLLFYIEG
                     LASRDVVNLIALRSVMLAERVPTLDASALGRDNIASVFMTHIVPSGQISVLSCIDKVV
                     EHVLTGEAALFIEGARQCVVFEAKGWEHRGIEEPHTEKVVRGPREGFSELLRANTALV
                     RRRLRTPDLRMDSTKVGRRSKTDIVIAYIDGLANPRLVATIRERIDHIDTDILPDSGL
                     LEEFIEDYPYSPFPQIQYTERPDRFCAGLSEGLVGIIVDGSPMALMAPGNLASFFQSP
                     EDYYERFPYGGPLRALRFIAGVIALTFPALYVAISLFHQEMLPTKLALAIAGSHMPVP
                     FPVLVEALLMEVALELIRESSVRLPDPVGQTMGFVGALLLGDAAVSAGLVSPIMVIVV
                     AVTGLASFAIPHYPTGLAIRLLRFPLLFLSAWLGLYGLMAGLMAIALHLGALSSFGVP
                     YLEPLMKPRPSLRDVVWRSPVFTFNKRPEYPEPLDEVRQKKFIRTWEPGVAEMARRES
                     QGGDEDGKDGGDQGDGGDRGR"
     gene            complement(3653..3829)
                     /gene="rpsU"
                     /locus_tag="PLM74_01395"
     CDS             complement(3653..3829)
                     /gene="rpsU"
                     /locus_tag="PLM74_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015044591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HON41769.1"
                     /translation="MSEIRVGKNESLDSALRRFKRQCQQSGVLAEIRRREHYDKPSVR
                     KKKKSEAARKRKFR"
     gene            complement(3978..4322)
                     /locus_tag="PLM74_01400"
     CDS             complement(3978..4322)
                     /locus_tag="PLM74_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012994994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HON41770.1"
                     /translation="MGDCIFCKIAAKEIPASLVYEDDQLLAFGDVNPQAPVHMLIIPR
                     EHIPSLGHVDSRHDQLVGRALRLAAKLAKGKGLGESGYRVVINSGPDAGQSVGHLHFH
                     VLGGRVMGWPPG"
     gene            complement(4326..5660)
                     /gene="mtaB"
                     /locus_tag="PLM74_01405"
     CDS             complement(4326..5660)
                     /gene="mtaB"
                     /locus_tag="PLM74_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HON41771.1"
                     /translation="MRRVAIATLGCKVNQYDSDALALEFSKAGFDVVPFGDRADVYVV
                     NTCTVTQMGDKKSRQLIRRAHARNPEAVIVATGCYAQASPREVAAIPGASVVSGTSDR
                     AGIVRAVIDLAENGGAPAMLVSEVGECRVYEELPSEGTAERTRAYIKIEDGCENFCSY
                     CKVPYVRGPVRSRDFEHIREEVRRLAARGFREIVLTGIDLGAYGRDFGGSPSLADVLE
                     MAANAQGIARVRVSSVDPTDVTDRLLDIMGSEPAVCPHLHVPLQSGDDEVLRRMNRRY
                     TGAEFEEVVARARARVPDLAVTSDVIVGFPGEDEPAFEATMSLCGRVGFSRLHVFQYS
                     RRRGTVAAGLAGQVAQEEKQRRSERLIALGRELALKFHRGLMGQTVQVLVEKSCGAVC
                     EGLTGSYVRVVASRATENGRSAPSPGAMAAVRVTEAQEGFVRGVVEGSAEGV"
     gene            complement(5691..6167)
                     /locus_tag="PLM74_01410"
     CDS             complement(5691..6167)
                     /locus_tag="PLM74_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HON41772.1"
                     /translation="MNVHTRYIARAAVIAGVYAGLCLLLAPISYKVLQVRVAEGLTLL
                     PMVWPEAVVGLTLGALIANTAGPFGIVDIILGTAATAAAAYLTYRYRGSRMAYFFPIL
                     VNGLVVGGYLPFVANMQIHAWTIPAAMLSVAAGEAVAVFAVGVPLLRALRRIGMIE"
     gene            complement(6267..6749)
                     /locus_tag="PLM74_01415"
     CDS             complement(6267..6749)
                     /locus_tag="PLM74_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41773.1"
                     /translation="MNRRISLIAIAVLLLVMATTLAGCSLGPPRISLTFSPNPVRLRV
                     DEPINATGTITLDGFGLFTVKSVILAYQDTDGGKVENPNLPAGGELDEAITLPGTMGF
                     FSVTKPLAELNNGEEIVPTLDWLAIYPRPVKIVFTFLDESGKPVGGGELRLEWVSDLV
                     "
     gene            complement(6895..7353)
                     /locus_tag="PLM74_01420"
     CDS             complement(6895..7353)
                     /locus_tag="PLM74_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41774.1"
                     /translation="MKRANILRLVAILAVVVLASMTTGCLKATITGKIQPDPLELTSE
                     AAIQGTLTLTMTGWLAGGMYEKLDVEFFKDGTDDPIAELTNLPINLLITPLNKVASVK
                     LQDIEGLAAPVDLWESDVLEATDAVFTVKPGAGYGLSPIVIEVEVTEPQG"
     gene            complement(7569..7913)
                     /gene="trxA"
                     /locus_tag="PLM74_01425"
     CDS             complement(7569..7913)
                     /gene="trxA"
                     /locus_tag="PLM74_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HON41775.1"
                     /translation="MATNSKPVVVTDATFESEIARHTGVSIVDFWAAWCGPCRMQGPI
                     IDNLASEYADGTVKVAKLNVDENPYSASQFGVMSIPTLIIFKNGKPVDKLVGVTPLPV
                     LKQHIGRASMQQ"
     gene            complement(8117..8869)
                     /locus_tag="PLM74_01430"
     CDS             complement(8117..8869)
                     /locus_tag="PLM74_01430"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008692.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HON41776.1"
                     /translation="MRRFFVPSDAIADGVVRIAGRDARHIVRALRMGHGDRLSIVDSS
                     GREYIARIAHTTETSVAAVIEAERESGPGEPRVPLTLAQGIPKLDKMDLIVQKCTEIG
                     VSRFLPVLTERSVARTNSDSAERRVLRWQRIAAEAAKQAGRGALPPVEDILGLEDALI
                     ELSSQGVLLVMPWELEQERGLRGALADIDLEKTPGIALIIGPEGGFSHYEVELAKRYG
                     ALTVTLGRRILRTETAGLAAATVIMYELGEFG"
     gene            complement(8892..9851)
                     /gene="prmA"
                     /locus_tag="PLM74_01435"
     CDS             complement(8892..9851)
                     /gene="prmA"
                     /locus_tag="PLM74_01435"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HON41777.1"
                     /translation="MNWAEVWVEVPDDRSELAAEVFRDAGAGGIAFTGPLLMRRLAEA
                     ANADGKLFPDNIAPGELIRVTAYYPADDLLESRLLSIQSALDAAFGIDPGVAIAAAGV
                     RPAICVAQVAGEDWAGAWKQYYHVDHIGQRVVIVPSWIDYTPAPGEAVVLLDPGEAFG
                     TGQHESTRGCIIALEESVVPGASILDVGCGSGVLSIAAARLGAGSLVGIDIDTVAVSV
                     ARNNVAANRVEDRVVIRRGDLATGVLQEFDVVVANILADVVIELMPELPRVLAPAGRF
                     ICSGFVEKHAARVEQALCTAGHRIIQRIQVEDWITLVSAAESS"
     gene            complement(9908..11050)
                     /gene="dnaJ"
                     /locus_tag="PLM74_01440"
     CDS             complement(9908..11050)
                     /gene="dnaJ"
                     /locus_tag="PLM74_01440"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006522623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HON41778.1"
                     /translation="MATATRDYYDVLGVERNASEADIKKAYWDLARKYHPDVNPDDPS
                     AESKFKEINEAYQVLSDEEKRARYDRFGPEDGAGVGGGWSPDFGGFDPFGSIFDAFFG
                     ESPFGRSARSGPARGHDLKQSVEITLEEAVAGAAKTVRVDRMGMCKSCGGEGTRPGTS
                     RATCPECGGRGQVQSVSSTPFGMFTRVTTCPRCNGEGRIIESPCDECRGAGRVREAVR
                     LEVEVPAGVDTGARIRVRGEGDSGTFGGPPGDLWVYVTVLPHSRFQRDGNDLRIEVPI
                     SFAQAALGDELTLQAIDGADVKVKVPAGAQSGAEYRVRGRGVPALRGFGKGDMVVRVT
                     VVTPTRLSVRERELFQELAELQSGGARPKGHNKKRRQGLFGRAEQG"
     gene            complement(11058..12119)
                     /gene="hrcA"
                     /locus_tag="PLM74_01445"
     CDS             complement(11058..12119)
                     /gene="hrcA"
                     /locus_tag="PLM74_01445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HON41779.1"
                     /translation="MAENMADRKKAILRAVTDDYITTAEPVGSRTIARKYGLGVSSAT
                     IRNEMADLEDEGYLEQPHASAGRIPSDKGYRFYVDTLMTGRDLTGIEEKRIRAEYERR
                     RDEIRALIRTTARMLGEISQCASVVVGPAARTAEIRHIQLVPLSSETVLVLVVTNSGL
                     VEHRVLSMEHAMHPSDLDRISHILNHRLRGSTLAQMRGAVLREIQAELSAYATFLDQM
                     FELLLAAMHSSDEERVYVDGLMQLLAQPEFKDVGRARPVLEFLESDDSVLQALADAGS
                     RGLGATLVTIGSENAVDELQSCSVISAPYGVAGRVVGAVAVVGPTRLDYAMATALVGY
                     MAEGLSDILARLDLCESGE"
     gene            complement(12606..>12791)
                     /locus_tag="PLM74_01450"
     CDS             complement(12606..>12791)
                     /locus_tag="PLM74_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41780.1"
                     /translation="SEGPVQTDTGWPKKVSRRVQDVISDDLPLPIVDADRISPCALAG
                     VCAAEDFATHDNSRWLP"
     assembly_gap    12792..12891
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12938..14260)
                     /gene="hemW"
                     /locus_tag="PLM74_01455"
     CDS             complement(12938..14260)
                     /gene="hemW"
                     /locus_tag="PLM74_01455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HON41781.1"
                     /translation="MGGVYVHIPFCVQKCAYCDFVSYPAPDIESLAPAYLHALLGELR
                     LRQDQLRSVAPIDTVYIGGGTPTCVPPHLLAAFLEQLLRAVRAVSAATDTVGPADDIE
                     VTVEANPGTVDHAGLAELRVAGANRLSLGFQSLADEELVALGRIHSAYDCLRAYCDAR
                     AAGFGNIGVDLILGAPGQTRGSLENAVAAVIDLGPEHVSAYCLSLEPGTPLADAARAG
                     AVSVPNDDDAADLYDLFVERITASGYARYEISNFARTGFESRHNMGYWRNGEYIGLGA
                     AAHSHVRVRAGAPVPASTTASALDPTTASAPTPGSFDFAASAPAPGGGIRSWNTPKVR
                     EYIDAVRRGDLPVAGREMRTAHDEAQDALMVGLRMSVGVDLGALSQEYGVDLVSEYAA
                     RFQRLASRGLVEIINSSLDRGTGSHLGPRIRLAPDAFLLANRVFVECV"
     gene            complement(14273..>15247)
                     /locus_tag="PLM74_01460"
     CDS             complement(14273..>15247)
                     /locus_tag="PLM74_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012811285.1"
                     /note="back-translocating Elongation Factor EF4; binds to
                     the ribosome on the universally-conserved alpha-sarcin
                     loop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HON41782.1"
                     /translation="TLASRPAAAPLPGYRPATPMVYSGLYPVEAAVYGDLRDALEKLK
                     LNDASISFEPETSAALGFGFRCGFLGLLHLEIVQERLEREYGLDLITTAPSVMYRVTE
                     TSGTSYLVDNPANLPPPNKIEVIEEPYVRAMVFVPSDFIGQVMDLVAERRGVMHDMQF
                     VSPTRVKAEYDMPLAEILYDFFDKLKSRTRGYASLDYEFLEYRPSNLVKLDIMVNGKP
                     VDALSAIVHYQFAERRGRALAERLRKLIPRQLFDVPIQAAIGGRIIARETVKATRKDV
                     LAKCYGGDISRKRKLLEKQKEGKRRMKQFGQVEIPQEAFMAVLGSDDE"
BASE COUNT         3096 a         4941 c         4169 g         2941 t
ORIGIN      
        1 ctccgtcttc ggactccacc aggcggaccc gtgccagaag aggttggtct ccaggctgct
       61 caatcgatgt aagcaagtcc agaaagcgcg accgcggcgc cgtcaacgag cgggccgtat
      121 tgcgcatgac atcatctagg aacaacgccg ccgagctcga ttgctcggtc tgggcgccaa
      181 gcacgtctcc cactggatca ctcgtcacaa atacccacga cttgtagcgg aattccctat
      241 cgcgggccaa cgcatcaagg gccctcgcga ctccaccgcg ggcgaaccgt tcgccaaaca
      301 ccagagccct cacatgggct cggaaaagca ccttggtcga cctaccattc agctggcgaa
      361 cagcatccca caccgtgtcg cccaccgacc tcactgtcca cgactcctgc ttctcccctt
      421 ctgcgggctt ggcagacggg agcgccactt gcgcccagac ctctattcgg ccgtctgccg
      481 tttcgtctac gcccaggccg agcacgaacc cgacactttc aatctcccgc gcgtctctgc
      541 atccagtgac ggacgccgca gccagcaggc acacaagcat ggcaacggta cgcccccacc
      601 gtcgtccggc ccgcgcagcg cctggcttcg tcttaacgcg catccttgcc acggacattc
      661 tcgccccctc ttcgcgagcc ccgaacgacg gcgcccacca gaagcacgac aggcaacgct
      721 atcgcatacc atactgagta tttcacgaac atgctcaggt aatcgaatgt ctccagcaca
      781 gtcttgggga tcaggctgag tgcagtgccc agcagcgcat agggaatgat gagggggcgg
      841 tagtcacgaa gcccgagtac gtcggcgacg gacgtgatcg atgcgtagaa caccgccgcc
      901 agtttcagta gggcagctga gaaccacagc gcgagaaaga gtatctccgc cctctcgagg
      961 aactgcccga agccgaccat cctagcagtc tcgagcaccg gaaacgtaac acgcgatatc
     1021 cctcggacgc ccagcatcat cactccaaac ccggtggcag tggccataac caccattccc
     1081 gcagccacgc ctgtagccat cgcccgggag gcgctgcgct tgtccttaag gtgcctgata
     1141 aacgccagaa ctacgacact ctctcctgca taggaagcgg ccagaaaccc accgtggatg
     1201 agcggcctaa gcccgttgcc gacaacaggc agcagacgtc cgggtaccac cacagggacg
     1261 gccagggcag tcaacaccag aagcgagatt accaccaggg tgaagaagat cagcgatact
     1321 cgggcaatgg cctcgattcc caggtaggcc gcatagatga tcagagcata gaagacgccg
     1381 cttatgatga ttgggggagt agtctgcaag aatgcgatcc atatcgccag cacagactcg
     1441 cgcgatccaa ggcccagcac cacggtgcag tacgcggcaa aggtgaggcc tatcagcttg
     1501 cccaacggag cgccgaatat cctctctgcc atgcggacaa ggctgtgatc aggaaagcgc
     1561 gccgccagat acccggcgag gacgactatg ccaaggccga tgaccccacc tatcaaagcg
     1621 gatatccacg ctgccgtgcc ggctacagga accagaatcc tcgccgcagc cagaaaccct
     1681 gtggcgaaga ctatcatcgc cgtgatcacg actatctgag tgtagtagat ctcatctttg
     1741 ggcctatcgc ccgcggtctc cgccgtcccc ttggtctccg ccgtccttgc cgtcttcatc
     1801 gcctccttgg gactccctac gtgccatctc agctactccc ggctcccatg tgcgtataaa
     1861 cttcttctga cgcacctcat ccagcggttc ggggtactcc ggtcgtttgt tgaaggtgaa
     1921 caccggcgag cgccatacca cgtcgcgcag tgaaggtctg ggtttcatca acggctcaag
     1981 atacgggact ccgaaggatg acagcgcccc cagatgcagc gcgatagcca tgagccccgc
     2041 catgagccca tacagcccca gccaggcaga gaggaagagg agcggaaacc tgagaagcct
     2101 gatcgccagg ccggttgggt agtgcgggat ggcgaaggaa gccaggccgg tcactgccac
     2161 cactatcacc atgattgggc ttacaagtcc ggccgacacg gccgcgtctc caagcaggag
     2221 agcccccaca aatcccatag tctgccctac cggatcaggt aatcgaacag acgactccct
     2281 tatcagctcg agggccacct ccatgagcaa ggcctcaaca agaacgggga acggcaccgg
     2341 catgtgcgat ccggcgatcg ctagggcaag cttagtgggg agcatctcct gatggaacag
     2401 ggatatggcc acgtacagcg cgggaaaagt gagcgcaatc actccagcga tgaacctcag
     2461 agcacgcagc gggccgccgt acgggaaccg ctcgtaatag tcttcgggag actgaaagaa
     2521 gctcgctagg ttgcccgggg ccatgagcgc catggggctt ccatcgacga ttatgcccac
     2581 aagaccctct gaaagccctg cgcagaaacg gtcggggcgc tctgtgtact gtatctgagg
     2641 aaaaggcgag taaggatagt cttctatgaa ctcctcaagc agaccggaat cagggaggat
     2701 gtcagtgtcg atgtggtcga tgcgttctct gattgtcgcc accagacgcg ggttagccag
     2761 cccgtcgata taggcaatga cgatatcggt cttggatcta cgacccacct tcgtcgagtc
     2821 catcctgagg tcgggggtgc gcaggcggcg cctgaccagt gcggtgttgg cgcgcagtag
     2881 ttcggagaaa ccctcgcgcg gcccgcgaac caccttctcg gtgtgcggtt cctcgatccc
     2941 cctgtgctcc cagcccttgg cttcgaacac tacgcactga cgcgcgccct cgatgaacag
     3001 tgccgcctcg ccggtcagaa cgtgctcgac gaccttgtcg atgcaggaca agacggagat
     3061 ctgccccgat ggaacgatat gcgtcatgaa cactgaggcg atgttgtcgc ggcccagcgc
     3121 actcgcatct agtgtgggca cccgctcagc aagcatcaca gaacgcagcg cgatcaggtt
     3181 gacgacatcg cgcgatgcaa ggccttcgat gtagaagagc aacccttcga tctcagggtt
     3241 ggtccagatg atcaggcggc ggataatcac atccgggcta ccttcgccca taaggtcgcg
     3301 gagtcgtcgt tcgacgcctt ccagcgtatc ggggatcggc ggttctggca gcgcagcatc
     3361 ggaatcacgc ggcgtcccgg catcctcgcc ctcggggccg gcggtcccga ttccgtccgc
     3421 gacctcttcc agtggcaaca gcggccgtcc cagcatcatc ctgggcctgc gcatgccgcc
     3481 gcgggccagt ctgcgaagga atctcactgc gaacactcct tcgtccgatg tcggtcttac
     3541 catatcaatc tgcccatgcg ggggaaaaat aatgtgggag agacaatcgt ctctcccaca
     3601 ccatgttcat tcgtgtttgc accgcactac ggggggtcag gctaccgtct gactagcgaa
     3661 acttccgctt tcgggcagct tcagactttt tcttcttccg cacgctaggc ttgtcatagt
     3721 gctcgcgcct gcgaatctcg gccaacacac ccgactgctg gcactgcctc ttgaaacggc
     3781 ggagtgcact gtctagcgat tcattcttgc cgactctgat ctctgacata cggatacccc
     3841 cctctccgtg cgagaatcaa ccaacaccac tgcacgtagc tccgtggatc cgtaccgtgg
     3901 accacgaggc aatcttgtca aattatactt cacgcgttcc ggcagtgtca atctcacccc
     3961 gacccggttt cgccggttca acccggcggc cagcccataa ctcgcccgcc gagaacgtga
     4021 aaatgcagat ggcctacgct ctgccccgca tcgggccccg agttgatcac gacgcggtaa
     4081 ccgctctcac ccaggccttt ccccttggcc aacttcgctg cgagccgaag tgccctgccc
     4141 acaagttggt catgacgtga atctacatgg cccaggctgg gaatatgttc ccgcggtatt
     4201 atgagcatgt gtacaggcgc ctgagggttg acatcgccaa acgccagcaa ctgatcgtcc
     4261 tcgtaaacca gggacgcagg tatctccttc gctgctatct tgcagaagat gcaatcaccc
     4321 atcattcaca ccccctctgc tgacccttct acgactcccc ggacaaaacc ttcctgggcc
     4381 tcggtgactc gcaccgcagc catcgcaccg ggcgacgggg ccgaacgccc gttttccgtc
     4441 gctcggctcg caacaacccg aacatagctg ccggtcagcc cttcacacac tgctccgcac
     4501 gacttctcca ccagcacctg cacggtctga cccataaggc cccgatggaa cttgagagcc
     4561 agttcgcgcc cgagggcgat cagccgttca ctgcggcgct gtttctcctc ctgcgccacc
     4621 tgccctgcta agcccgccgc aacggtgcca cggcgcctgg aatattgaaa tacgtgaagg
     4681 cgcgagaatc ccacccggcc acacagcgac atggtcgcct caaaggctgg ttcatcctcg
     4741 cccgggaatc cgactatcac gtcgctggtc acggcaagat ccggaacgcg cgcgcgggcg
     4801 cgggctacaa cctcttcgaa ttcggcgccg gtgtagcgac ggttcatccg ccttagcact
     4861 tcatcatcgc cggattgcaa cggcacatgc agatgcggac agactgcagg ttcagacccc
     4921 ataatatcga gaagcctatc ggtaacatcc gtgggatcta ccgaacttac gcgcacgcgc
     4981 gcaatgccct gggcgttcgc tgccatttcg agtacatcgg ccagactcgg cgatccccca
     5041 aagtcgcgcc cgtaggctcc caggtcgatt cctgtgagca ctatctcgcg gaacccccgg
     5101 gcagccaggc gccgtacctc ctcgcggatg tgttcgaaat cccgacttct caccggaccg
     5161 cgcacatagg ggaccttgca gtacgagcag aagttctcgc acccgtcttc aatcttgatg
     5221 taggcgcggg tgcgctcggc cgtgccttca gagggaagct cttcatagac gcggcactcg
     5281 cctacttcgg agacgagcat ggccggagcg cctccgttct cggcaaggtc gataacggcc
     5341 cggacgatgc cggcgcgatc cgacgtgccc gacaccaccg aggcccccgg aatcgctgcc
     5401 acctcgcggg gcgacgcctg cgcatagcag cccgtcgcca cgatcacggc ctcaggattt
     5461 ctggcgtggg ccctccgaat tagctgacgc gacttcttat cgcccatttg cgtaacagtg
     5521 caggtgttca ccacgtacac gtcggccctg tcgccaaacg gtaccacatc aaaaccggcc
     5581 ttgctgaact ccagtgcaag cgcgtccgag tcatactgat tcactttgca tccaagggtg
     5641 gcaatggcaa ccctgcgcac agtcagtctc cttttgtgtc tgttcttcgt ctattctatc
     5701 atgcctatcc tgcgcagggc tcgaagaagc gggacgccaa cggcgaacac ggcaactgcc
     5761 tcgcccgcag caaccgacaa catcgccgca gggatggtcc atgcatggat ctgcatgttc
     5821 gccacgaagg gaagatatcc gcccactacg aggccattga ctagtatggg gaagaagtac
     5881 gccatacgcg aaccgcggta cctgtaggtc aggtaggcgg ccgctgccgt ggccgccgtc
     5941 ccgagaatga tgtcgactat cccgaaaggc ccggctgtgt tggcgataag cgcgcccagc
     6001 gttaggccta ctacagcctc gggccacacc atgggaagca gcgtaagtcc ctcagccacc
     6061 cgcacctgga ggaccttgta ggaaatgggg gcgagaagca ggcacaggcc ggcatacacg
     6121 cccgctatca ccgctgcgcg ggctatatac ctagtatgta cattcattct cgcatcccgc
     6181 ccccttgtca tgctgccacg agactcctca gggaaacctc taggtgcggt cctaacttgc
     6241 tttattgttc gccgctactc cgaaggttac accagatcac tgacccactc cagcctgagc
     6301 tcgccgccac caacaggttt tcctgattcg tcgaggaatg tgaacacaat cttcacaggt
     6361 cgcgggtaga tcgccaacca gtctagggtg ggcacgatct cctcgccatt attgagctcg
     6421 gcaaggggct ttgtaacgct gaagaagccc atcgtccccg gaagcgtgat cgcctcgtcc
     6481 agctcgccgc ctgcaggcag attagggttc tctaccttcc ccccgtcagt atcctgataa
     6541 gcgaggatga cactcttcac cgtgaacagc ccgaaaccgt caagcgtgat ggtcccggtc
     6601 gcattgatgg gctcgtcgac cctcagtcgc accggattgg ggctgaacgt cagtgaaatc
     6661 ctcggagggc ctaagctgca ccccgcaagt gtggtggcca tgaccagcaa gagcactgcg
     6721 atagcgataa gcgaaatacg tctgttcaat tgctccacct ccagcaggcc tcttattgga
     6781 gtcggatgct cgcaaagagg tcgttaccgt atatatcggc aaatccgtgg aggaaataag
     6841 gcaggggccc gagtagcgta gactgcccgg gacccctcat ggacgaacaa gcatttagcc
     6901 ttgaggctcg gtaacctcga cctcaatgac tatcgggctt aggccgtagc ctgcgccagg
     6961 tttgactgtg aagaccgcat ccgtagcctc gagcacatcg gattcccata gatccacagg
     7021 tgcagccaaa ccctcgatat cctgcaactt gacagatgcg accttgttaa gcggtgtgat
     7081 cagcaggttg atcggcagat tcgtaagctc cgcgatagga tcatcggttc catccttgaa
     7141 gaactccaca tcgagtttct cgtacatccc gccggccagc cagccggtca tagtgagagt
     7201 cagcgtaccc tgaatagccg cctcgctcgt aagctccagc ggatccggct gaatcttgcc
     7261 cgtgatagta gccttcaagc atccagtggt cattgaggcg agcaccacga ctgcgagtat
     7321 ggccacgagt ctcaagatgt ttgccctctt catgtttcgg ccctcctcct tctagtgggt
     7381 tgtggtggag atctttcccc ctgcaaacct tgtgggagta ttcgctcacg cgtggtcgat
     7441 tcctgccggt attctcgata attgggggaa ttatcaggcc cgtacagcaa cgagcccgcc
     7501 tccagcccgg cagagggggc atggaagcgg gcacatactc atgaggcgca ggttctgtgt
     7561 gccctctgct attgctgcat tgacgcccgt cctatgtgct gcttaagcac gggtagcgga
     7621 gtgacgccca ctagtttgtc gacgggtttg ccgtttttga agataatgag cgtagggatg
     7681 ctcatcaccc cgaactggct ggccgaatag ggattctcgt caacgttcag cttcgccacc
     7741 ttgacggtgc cgtcagcata ttcactcgcc agattgtcga ttatcgggcc ctgcatccga
     7801 cacggcccgc accaagcagc ccagaagtcg acaatcgata ccccggtatg cctcgcaatc
     7861 tccgactcaa aagtagcgtc agtaacaaca acaggcttac tgtttgtcgc catgtaaatt
     7921 ccgatcctcc cttacagatc ccgccccggc ggcgcaccgc gcgcgaatgc cttcactatt
     7981 gtgcgcatcg tgccctccgc taactcctgt atacggtagg ggggtatccg ttgagactat
     8041 tatatggtgg catggtccgt atgtcaacat actctaccag cacatactcc acccaccaga
     8101 cccgtgccca gccaggctac ccaaactccc ccagctcata cattatgacc gtagctgcag
     8161 ccagacccgc cgtttcggtg cggagaatcc gccgcccaag tgtcaccgtc aaggctccgt
     8221 aacgtttggc tagttccacc tcgtagtgtg agaatcctcc ctcgggtccg atgattaggg
     8281 ctatcccggg agttttttcg agatcgatgt cggccagcgc tcctcggagg cctcgctcct
     8341 gttcgagttc ccacggcatg acaagtagca cgccctggga cgaaagctcg ataagggcgt
     8401 cctcaagccc gaggatatcc tctaccggcg gcaatgcgcc gcggccggcc tgcttggcgg
     8461 cctccgctgc tatccgctgc caccggagca ccctgcgttc agccgaatcc gagttggttc
     8521 gggctacaga gcgctcggtt aacacgggca ggaatctgga cacgccgatc tcggtacact
     8581 tttgcactat gaggtccatt ttgtcgagtt tggggatgcc ttgcgctaag gtcaggggca
     8641 cgcgcggttc gcccggtcct gattctcgct ctgcctcgat tacagcagca acgctcgtct
     8701 cggtggtgtg cgcaatgcgg gctatgtact cgcgcccaga gctgtccact atcgacagtc
     8761 tgtcgccatg ccccatcctg agcgcacgca caatatgccg ggcatcgcgc cccgctatcc
     8821 gaaccacgcc gtctgcgatt gcatcagacg gcacaaagaa cctgcgcata tcgtctgtct
     8881 ccaagaatag gtcaagatga ctcggccgcc gagaccagag taatccagtc ctcaacctga
     8941 atgcgttgga tgatgcggtg accggccgtg cagagtgcct gctcaacccg ggcagcgtgt
     9001 ttctctacga aaccggaaca aatgaaacga ccggccgggg cgagcacgcg cggaagttcc
     9061 ggcatcaact cgattaccac gtcggccaga atgttggcca caactacgtc gaattcctgc
     9121 aacacgcccg tggcgagatc gccccggcgg atgaccacac gatcctccac tctgttcgca
     9181 gcaacgttgt tccttgccac gctcaccgca acggtgtcga tatcgatgcc taccaagcta
     9241 ccggcgccca gcctagcggc ggctatggag agcacgcccg aaccgcagcc tacatcgagt
     9301 atgcttgcgc cgggcacgac tgactcctcc aaagcgatga tgcatccccg ggtgctttca
     9361 tgctgcccag ttccgaaagc ctctccggga tcgaggagca ccacggcctc gcccggtgca
     9421 ggagtgtagt ctatccacga tggcacaatc acgacgcgtt gaccaatgtg gtcgacatgg
     9481 tagtactgct tccaggcgcc ggcccagtct tcgcccgcta cctgcgccac acagatggcg
     9541 ggacgcacgc cggcagcggc aattgcaact ccggggtcga tgccgaacgc ggcgtccaga
     9601 gccgactgga tggatagaag ccggctttcc agtaagtcgt ctgcgggata gtaagccgtt
     9661 actctgatca gctcaccagg tgcgatgttg tccgggaaga gcttgccgtc tgcgttagcg
     9721 gcttcagcca accggcgcat caacaagggg ccggtgaatg cgattccacc agccccggca
     9781 tcgcggaaga cctctgcggc cagctcactg cggtcatcgg gtacttcaac ccacacttcc
     9841 gcccagttca cccagtcacc ccatccgtct cggtcttgtc tcgatctcga gcgcatgtac
     9901 gaacgcgtta tccctgctcg gccctaccga acagaccctg acgtctcttc ttgttgtggc
     9961 ccttgggccg agcgccgcct gactgcagct cggcaagctc ctggaagagc tctctctccc
    10021 tgacgctgag gcgtgtaggc gtcactactg taacccggac gaccatgtcg ccttttccga
    10081 acccgcgcag cgcaggcact ccgcgcccgc gcacacgata ctcggcgccg ctctgagcgc
    10141 cggctggaac cttaaccttc acatccgcgc cgtcgatggc ctgtagggtc agttcatcgc
    10201 ccagagcagc ctgagcaaat gatatgggga cctctatgcg caggtcattg ccgtcgcgct
    10261 ggaaccgcga atgaggcaag accgtgacgt acacccacag gtcacctggc ggaccgccga
    10321 acgtgcccga atcaccttcg cctctcacac gaatccgggc cccggtgtcc actccggcag
    10381 gcacctctac ctcaaggcga acagcctcgc gcacacgccc tgcgcctcgg cactcatcgc
    10441 acgggctttc gatgatcctg ccttcgccgt tgcagcgcgg gcatgtggtg acccgggtga
    10501 acatcccaaa aggagtgctg ctcactgatt gcacttggcc acgcccgccg cattctgggc
    10561 atgttgccct ggatgtgccg gggcgtgtgc cctcgccgcc gcaagacttg cacatgccca
    10621 tgcggtctac gcgcactgtc ttggcggcgc ctgctactgc ttcctccagt gttatctcaa
    10681 cagactgctt gaggtcgtgg ccacgcgccg ggcctgaacg ggcagagcgg ccgaacgggc
    10741 tttcgccgaa gaatgcatca aatatgctgc caaatgggtc aaaacctccg aagtcgggtg
    10801 accaacctcc cccgacccct gccccatctt ccggcccgaa cctatcgtat cgcgctcgct
    10861 tttcctcatc ggaaagcacc tgataagctt cgtttatctc cttgaacttc gactcggctg
    10921 aagggtcgtc aggattcaca tccggatgat acttgcgggc caggtcccag taggcctttt
    10981 tgatgtccgc ctccgatgcg ttccgttcca ctccaagcac gtcgtagtaa tcacgcgtcg
    11041 ccgtcgccaa caccgcctca ttcgccagat tcgcacaagt ccagccgggc caagatgtcc
    11101 gacaggcctt cggccatgta ccccacgagc gcggtcgcca tcgcgtagtc cagtcgcgtg
    11161 ggccccacaa cagcaacggc acctactacc ctgccggcca caccgtacgg cgcactgatc
    11221 acactgcacg actgtagttc gtccacggcg ttctcagaac cgattgtgac cagcgttgcc
    11281 ccaagtcctc gcgaacctgc atcggctaat gcctggagca ctgagtcgtc ggattccaag
    11341 aactccaaca cgggccgggc gcggcctaca tccttgaact ccggctgtgc aagcaattgc
    11401 atcagcccat caacatatac ccgttcctca tctgacgaat gcatcgccgc aagcagcaac
    11461 tcgaacattt gatccaggaa cgttgcatac gccgaaagct ccgcctggat ctcgcgcaac
    11521 accgctccgc gcatctgcgc cagtgtgctt ccgcgaagcc tgtggttgag tatgtgcgat
    11581 atcctgtcca ggtcagatgg gtgcattgca tgttccatcg acagcacacg gtgctcaact
    11641 aggcccgaat tggtcaccac caagacgaga accgtttcgg agctgagcgg aacgagctgt
    11701 atatggcgta tctcggccgt ccttgccgca ggccccacca ccactgaagc gcactgagat
    11761 atctctccca gcatcctggc ggtggttctg ataagcgcac gaatctcatc ccgacgtctc
    11821 tcgtattcgg ccctgatgcg cttctcctca atcccggtca gatcccttcc ggtcatgaga
    11881 gtatccacat agaaccggta gcccttatcc gagggtatgc ggcctgcgga tgcatgcggt
    11941 tgctcgagat agccctcatc ctcaaggtca gccatttcgt tgcgtatcgt agccgaactg
    12001 accccaagcc cgtactttcg tgcgatggtt ctcgaaccca ccggctccgc tgttgtgatg
    12061 tagtcatcgg tcaccgcacg aagaatcgcc ttcttgcggt ctgccatgtt ctccgccatc
    12121 gcttctcacc ccttgttcgc tcctgcagcc ttcacagcct tcctcgcgac cgccgcgctc
    12181 gtcacactgg caggatccct tgtgctcgct ggcttcctcg cgctcgccgg attcctcatg
    12241 ctcgccgccc ggacggtatc gaaagcccgc atcaacaggc caaagttagc actcgtaggc
    12301 tgggagtgct aatgaaaaca tagcactcca ccctgtccat gtcaagattc ggacgaccgt
    12361 acggcgacgg ctccgcagct taagccgggt tacgtttcgt gcggacgtgc atgtacgggc
    12421 tgccaacgcg agcagcacga ggctgagaat gtcgcccgat tctgcccgcg ggccttcggg
    12481 aaggcggtgg cgctgtacca tatgcggtac gcaatcacat ccggtgccct acgggacacc
    12541 tttttcggcc atcctgtgtc ggtttgcaca ggcccctcgg aaaccctggc ccacagggca
    12601 caggttcatg gaagccatcg gctgttgtcg tgcgtggcga aatcctcggc tgcgcaaacc
    12661 ccagctaggg cgcatgggct gattcggtcg gcatcgacta tcgggagcgg aaggtcgtcg
    12721 ctgatcacat cctgtaccct acgggacacc tttttcggcc atcctgtgtc ggtttgcaca
    12781 ggcccctcgg annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naaccctggc
    12901 ccacagggca caggttcatg ggagcgggag gaactgccta gacacattcg acaaacaccc
    12961 tgttcgccag caagaaagcg tcaggcgcga ggcgtatacg cgggcccaag tgcgaacccg
    13021 tgccccgatc caagctcgaa ttgatgattt ccactaggcc gcgtgaagcg agtcgttgaa
    13081 aacgtgcagc gtattcgctc acgaggtcga ctccgtactc ctgcgatagt gcacccaggt
    13141 ccaccccgac cgacattctg agtcccacca tcagggcatc ctgggcttcg tcgtgcgcag
    13201 tgcgcatttc gcggccagcc acaggcaggt cgccacgcct tacggcatca atgtattccc
    13261 tcacctttgg agtgttccac gaccgaattc cgccgcccgg cgctggcgca gaggctgcga
    13321 aatcgaaaga gcctggtgtc ggggccgagg ccgtggtcgg gtccaaggcc gaagccgtgg
    13381 tcgaggccgg cactggtgct ccggcgcgga cgcgaacatg cgaatgcgca gcagcgccca
    13441 gccctatgta ttctccgttg cgccaatacc ccatgttatg tcggctttcg aaaccggtcc
    13501 gggcgaagtt ggatatctcg taacgtgcgt agcccgaagc agttatgcgc tccacaaaca
    13561 agtcgtaaag atcggcagca tcatcatcgt taggaacact caccgcccca gcccgagcag
    13621 catcagcgag tggtgtgccc ggttccagcg acaagcaata agcggacacg tgttcgggcc
    13681 ctaggtcgat caccgcggca accgcattct ccagcgagcc gcgggtctgg ccgggggccc
    13741 ccagtatgag atctacgcct atgttgccaa agcccgcggc gcgggcgtcg caataggcac
    13801 gtaagcaatc gtaagcagag tgtatgcgtc cgagagcaac cagctcttcg tcagccaatg
    13861 actggaagcc cagcgacaat cggttcgctc cggccacgcg cagctcagcg aggcccgcat
    13921 gatccacggt tcccgggttg gcctccactg tgacctcaat atcatcagcc ggcccaactg
    13981 tgtcagtggc cgcgcttacc gcacgaacgg cccggagcag ttgctcgaga aaggctgcga
    14041 gcagatgggg cggcacgcaa gttggagtgc cgcctccgat gtacactgtg tcgatagggg
    14101 ccacactacg aagctgatcc tgcctcagtc ggagttcgcc gaggagtgca tggagataag
    14161 ccggcgccag cgactcgatg tccggcgccg ggtacgacac gaaatcgcag tacgcgcact
    14221 tttgcacgca gaatggtatg tgcacgtaca ctccgcccat ggcccaccac acctactcgt
    14281 cgtcagaccc cagtacggcc atgaaggcct cctgcggtat ctccacttgc ccgaactgct
    14341 tcatgcggcg tttgccctct ttctgctttt cgaggagttt ccgtttgcgg gatatgtcgc
    14401 cgccgtagca cttggccagc acatccttgc gcgtggcctt gacggtctcg cgggctatga
    14461 tgcggccgcc gatggccgcc tgaatcggca catcgaagag ctgtcgcggt atcagcttgc
    14521 gcaatcgctc tgctaaggcc cgaccgcgcc tctcggcgaa ctgataatga acgatggccg
    14581 acaaagcgtc gacaggcttg ccgttcacca tgatgtccag cttcacaagg ttcgatggcc
    14641 ggtattccaa gaactcatag tcaagagacg cgtatccccg cgtccgcgac ttgagcttgt
    14701 cgaagaagtc gtacaggatc tcggcaagag gcatgtcata ctcggccttc accctggtag
    14761 ggctgacgaa ctgcatgtcg tgcatcacgc ctcgcctctc cgctacaagg tccatcacct
    14821 gaccgatgaa gtcggacggc acgaagacca tcgccctcac gtaaggctct tctatgacct
    14881 cgatcttgtt aggcgggggc aggttggcag ggttgtccac cagataggac gtgcccgagg
    14941 tctcagtcac tcggtacatg actgaagggg cagtagttat caaatctagc ccgtattcgc
    15001 gttccagcct ctcctggact atctcaaggt gcagcagccc gaggaagcca cagcgaaacc
    15061 cgaaccccaa ggcagccgag gtttcaggct caaacgaaat cgacgcatca ttgagtttga
    15121 gtttctccaa ggcatcgcga aggtcgccgt acacagcggc ctcgacgggg tacaggccgg
    15181 agtataccat aggagtggct ggcctatagc cgggaagcgg cgccgctgcc ggacggctgg
    15241 ctaaggt
//
LOCUS       DAOTAC010000017        15016 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_3344_length_15016_cov_5.799679, whole genome shotgun sequence.
ACCESSION   DAOTAC010000017 DAOTAC010000000
VERSION     DAOTAC010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 15016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15016
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3344_length_15016_cov_5.799679"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="PLM74_01465"
     CDS             <1..525
                     /locus_tag="PLM74_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HON41783.1"
                     /translation="TENPLDLVIEGDGFFQVLLPDGSTAYTRTGAFKVDSEGRIVTSD
                     GFVMEPELVVPANAVDISIGTDGTVTIQVAGENAPTELGQIQLARFLNPGGLVSIGRN
                     LYKPSAASGDPAVGTPGLEGIGTIAQKFLEMSNVKVVEEMVNMIIAQRAYEVNSKAIQ
                     TSDEMLAAANNLRR"
     gene            561..1442
                     /gene="flgA"
                     /locus_tag="PLM74_01470"
     CDS             561..1442
                     /gene="flgA"
                     /locus_tag="PLM74_01470"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HON41784.1"
                     /translation="MRRAAVFLSISRVATCCAAVCRVVLCCVVLCCAGLYWAVPCRAE
                     SESEPPIDMTIEFASPAYTAGPQILLGDIARVHVLDDALWARLAQLEVGPAPIPGGSR
                     KVNVGQVQVRMRQQRIDPAQFSFQGPAEISVTAVAERVAGSRIEQAAIEAVCAHAGHA
                     LEPEDVKVVRFDYPQGLTVPPGPVVLEASIVPPSADAALVRANVKVIVGGTAARTVPV
                     WLEITAPPMVARGNSVLLVAEGPGIHVTVSAVALEDGRPGQRIRVRNAASGVEAAGRV
                     MGPGTVQVTLSAMGGVL"
     gene            1448..2101
                     /locus_tag="PLM74_01475"
     CDS             1448..2101
                     /locus_tag="PLM74_01475"
                     /inference="COORDINATES: protein motif:HMM:NF014195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HON41785.1"
                     /translation="MRISARGAAAVALIAITTAAVAVAMCPTAATATSLYAAGAAQSG
                     FSGMYCDYKARQVGDLLTIMVTEVSYAQNRTDTQTQKGIGANLSPDGAVFDFLKAVKL
                     DAGAGLNLGNSSKGGTTTSRSGKLIGTITAQVVEVLPNGDLRIRGVREVSVNREKEQM
                     IVTGIVRQRDISPGNTIPSTMVADAQIAFSGDIYTGEKGGIFKTLWDGLVYLWNWIF"
     gene            2121..3425
                     /locus_tag="PLM74_01480"
     CDS             2121..3425
                     /locus_tag="PLM74_01480"
                     /inference="COORDINATES: protein motif:HMM:NF014206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HON41786.1"
                     /translation="MAISLDWVKQAGGPLKTRAGKPGPTDGSTWESFSIVSDNELQRA
                     PGRLTSQAIANILAILLALVLMILTALMMASGSVCAAETGPGGSGVTVRIKDITRIYG
                     VRGNQLIGYGLVVGLRGTGDSRSAPYTARSIANMLEKFMITVDPSEVKSKNIAAVMVT
                     AVLPPFAVPGDTLDIVASSIGDAKSLLGGTLLLTELSGLDSKVYAMAQGQIIAGADAV
                     TTVGRIPSGATVEGYVPMEMVSPEGYMVLSLINPDYTTAARIADAVCARFGSGSAVAI
                     DGGSVRVTVPESFRQMPTPFAAEVGELQVKPDAPARVIVNSRTGVVVVGANVKISPAA
                     VSYNGFNVRVGAATDEAAVAAAAAAGAAGGVGVGVGSAARPAGGAAGGAAIGSTVLLE
                     AGSTIQTLVAALNAVGARPRDIMSIMEALKACGALQAELEIM"
     gene            3448..3819
                     /locus_tag="PLM74_01485"
     CDS             3448..3819
                     /locus_tag="PLM74_01485"
                     /inference="COORDINATES: protein motif:HMM:NF021638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HON41787.1"
                     /translation="MTADVVVVRNMGRSLADVGRRGNPGGMSSSGVSGGAGDRVDDPK
                     LIQACRDFEAIFIETVLKSARAGLPKDGLFSSHEQEIVSGMLDAEMARAMAHGRGMGL
                     AEFILRDITGREKRVTTNAPR"
     gene            3806..4249
                     /locus_tag="PLM74_01490"
     CDS             3806..4249
                     /locus_tag="PLM74_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HON41788.1"
                     /translation="MPLDNCARCGKLFNRLSRPICPDCAREEERQADVVMEFLKDNPS
                     ATIEDISESTGVDSQIILRLIRDNRLRATGAYSVPRNCKACGAPIDAGQYCGHCLRKF
                     GQAFAGKGIGGGGGSGDLRGRLSDADRARMARMATPERRRGDRKS"
     gene            4393..4716
                     /gene="flgM"
                     /locus_tag="PLM74_01495"
     CDS             4393..4716
                     /gene="flgM"
                     /locus_tag="PLM74_01495"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HON41789.1"
                     /translation="MKVTGSRGFDPVKLYSQTTGKANRADRQGEARPDEVSISDEGKV
                     MHHAMQALSSIPDVREEKVQSVRQRAAAGTYEASGKEVVKKLFSRAVRRGDLGGTRNR
                     TDENT"
     gene            4682..5185
                     /locus_tag="PLM74_01500"
     CDS             4682..5185
                     /locus_tag="PLM74_01500"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HON41790.1"
                     /translation="MEHATELMRILDEELSLCSELLYVTRAQKPAIISGDVLGLAELV
                     ARAEETIRRLRDVELSLADVASEFAFESSGKPADDLDSAIATFVASLDDAQRTEFQKT
                     KSRIAGLLDDIAAANAVNAGLLRDALGYIDNTVRLIAGADGDSSIYSRLGMLDRKASL
                     AAVDETA"
     gene            5208..6542
                     /gene="flgK"
                     /locus_tag="PLM74_01505"
     CDS             5208..6542
                     /gene="flgK"
                     /locus_tag="PLM74_01505"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HON41791.1"
                     /translation="MLRSSFTGLETARRALFAQRMAIDTTGHNIANANTPGYRRQRAE
                     MVATPPAPGPMQPGTGVKVATIERMRDHFVDMQIRHSHASLGRWEARSDCLEQLQEVF
                     KEPSEIGLSTMMDRFWIAWQELSGGATDPGRRTAVIQEASTLASAFGLISRQLKEASD
                     DIQVNIDSLVHSVNATAESIARLNHEIVRGGVVGENVSDLMDQRDMLLDQLARDVNIN
                     VVETPEGQVNVSVGSRLLVSGVTAFKMDVSGSFAGGRIQGLLEAKNEIIPSYQAKLDQ
                     LAQALKDTVNAQHSAGVDMYGDAGLGFFTGTGAADLAVSDALKNDPNKIAASRSGTGP
                     GDGSNALAMAQLKDALAIGTPPTASFRDFYGSIITELGLATQESSRAADVYNGVLEQQ
                     QMRRDAVSGVSLDEELMQLIRYQAAYDAASRLVSVMDEMLETLINRTGIVGR"
     gene            6567..7481
                     /gene="flgL"
                     /locus_tag="PLM74_01510"
     CDS             6567..7481
                     /gene="flgL"
                     /locus_tag="PLM74_01510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HON41792.1"
                     /translation="MRVTNSTMINKAIRNVDGAFRRYIACQEKVASGRSINHLSDDPH
                     GAARAVRLRSVLRGVEQYKRNAENADTSVRTYDDNLDKVGSILNRVRELVVRGATDSA
                     TPTEREAMAVEVRELFDELVHAGNASHLGAYMYAGHRTGKSPFEVIDDMGDANWPVSY
                     AGDSGVNRVEVGPGLTLEINITGEQVFMGQSGGVNIFRTVRQIEQHLTESDVPKLSGS
                     DLASLDQAINQVLKFRAELGGRGSRLELVINRLESDSVSITELLSKTEDVDLADAIMR
                     LAEQDVVYQAALAAAARSVQAGLLQYLK"
     gene            7506..7988
                     /locus_tag="PLM74_01515"
     CDS             7506..7988
                     /locus_tag="PLM74_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005990293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HON41793.1"
                     /translation="MEQAIAGAEMQLVVFELGDESYGVDISRVQDINRMQEITEIPHA
                     PESVVGVINLRGRVIPVIDLRKRFGLPEAPHTKDTRIVVVHLDDNLIGVIVDAVSQVL
                     RIPADIVEPPSPVLAGVDSRYLRGIAKLDEKLVILLDLDFVLSRREQEAISEVLSEEV
                     "
     gene            8000..10174
                     /locus_tag="PLM74_01520"
     CDS             8000..10174
                     /locus_tag="PLM74_01520"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HON41794.1"
                     /translation="MLQFPGMTEDDIKVFLAEADEQLQYLEDGLLDLEQSSEDPEVIA
                     QIFRAAHTLKGSSATLGHDKMAKLTHSMESVLDLVRKSQMNVTPALMDLMFECLDMLR
                     ILNREIATAQDSGADITELDDRLLDVIVSVERQATSGDTMGGVAAGENTPSIGGASSE
                     DAWEGQFTGSGDVHVKVRFAKTCPMPSVRAYQVIDRLSTLGVLVCSKPSVERIEAGDD
                     VFDLVAVVEYDGDALEPLIETIRSVTDVEDVRVLQVSPVGSTRAAIQADAAPQAADAA
                     PATAAETVTAKLAVSREGATGGAARTVAGASPSRAATAAAGTTTVRVDVARLDALSNL
                     VAELVIDRTHLAQLESRLAEKHGGDELVSELNRTSTHIGRLTTELQEAINKARMLPID
                     NVFKRFPRMVRDLAQKQGKEVELIIHGEDTELDRSVSEEIGDPLIHLLRNAIGHGIEP
                     PEERVRAGKPPRGVVRLSAFRQENYIVVQVADDGRGIDPELVRASAIRKGIITEDAAA
                     RLSDKDAINLVFSSGLSTAAKADDVSGRGVGMDIVRKNLEKLNGNISVDTALGRGSTF
                     TVKLPLTLAIIRALLVSLGQRMYAIPLNSVEEITRVEKSDISTVGGSEAIRPRGEVVP
                     LLWLGEVFGVRDPGGSAHLSVPGQKVFAVIASSHEGKVGLAVDRLVGEMEIVIKSLGT
                     FVGNISGVSGVTILGDGRVALIVDVPSLVRSVVDERNAVSQC"
     gene            10146..10667
                     /locus_tag="PLM74_01525"
     CDS             10146..10667
                     /locus_tag="PLM74_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HON41795.1"
                     /translation="MNETLLASANVIPVGIGELHVTDVQDIEMVAYGLGSCVGVVIYD
                     EAARAGGMAHVLLPTRSWGCGLPDTPDVHARYADTGVRELLDRVTDLGASRRRLVAKI
                     AGGARMFQVAGDTDMLDIGARNAEAVRAALAETGVPIIAEDVGGTVGRTMRYLVGSGK
                     VYVREPGMAEKEL"
     gene            10689..11045
                     /locus_tag="PLM74_01530"
     CDS             10689..11045
                     /locus_tag="PLM74_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HON41796.1"
                     /translation="MPTVLVVDDAAFMRMRAGKLLAQHGYRVLEAENGLDALNIYMQH
                     KPDLVLMDITMPVMDGITALKEIRQADPQAKVVMLSAMGQQAMVIESIKAGAKDFVVK
                     PYEPDNVMASVRKLIG"
     gene            11045..12130
                     /locus_tag="PLM74_01535"
     CDS             11045..12130
                     /locus_tag="PLM74_01535"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HON41797.1"
                     /translation="MAQISVLVADDSAFMRKVVSDLIAADPELVVVGTARNGSDCIQK
                     ARSIRPDVITLDVEMPVMGGLEALPTLTTELGIPVVMLSSLTQAGADVTIRALSLGAV
                     DFVPKPSGAISLDIAAVRDELVRKIKAAAAISAGLVRTLARDLRTPALGPAPVSMPTT
                     PTTPTRPSTARAAARKVVVIGTSTGGPRALTQVIPWLPPNLPAAVLIVQHMPAGFTRS
                     LAERLNDLSRIRVTEAQGGETIAEGEALIAAGGRHMLVAADGALVLDDSPPMHGVRPA
                     VDMTMNSAVDVFGRRIVGVIMTGMGSDGADAMARVKLAGGKTIAEHESSCVVYGMPRS
                     VVERGIADKVVPLPEIADEIVNAVLSV"
     gene            12199..13011
                     /locus_tag="PLM74_01540"
     CDS             12199..13011
                     /locus_tag="PLM74_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006192297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HON41798.1"
                     /translation="MDYVNTADYEAFRRKVISLTGIDLGLYKSQQMQRRIRSLLDKVG
                     TPTFTHYGSLLERDPEELRRFCDYIMINVSEFFRNPEKFDELEKEILPSLLRRSPNLS
                     IWSAGASNGAEIYSVAMILDDIAPKGYHKLLATDIDSGAIAQALAGVYGPQDVKNVSS
                     ARLARHFTCEGSKYTISSRIKSRVQFRMHNLLADRFERGFDLILCRNVVIYFTDEAKQ
                     TLYAKFRESLRDDGVLFIGGTETILNAREIGFLPTSSFFYRRAPLGKEAAAQ"
     gene            13008..14045
                     /locus_tag="PLM74_01545"
     CDS             13008..14045
                     /locus_tag="PLM74_01545"
                     /inference="COORDINATES: protein motif:HMM:NF024878.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HON41799.1"
                     /translation="MIRVLSAQVRPGMKLAEHISNQAGVVQLRAGTVLDENGARRLSE
                     VGFGSIAVEYPGTDDVVVTHTVPRKLMDHGRELLRAFYDHVLGAGAPENVRVPFGELL
                     ELVQAVEESLEFVNQNAIEVCPLTTADEFDYVVPLNVAVVSMFVAAKAGLAKRSHDVG
                     LGALLCNIGTALTDDPARHPEMSLRVLRQDQRFSAYSKAIVFQHEERHDGSGYPRGLA
                     GDDIEPLARMVAVADMYCSLIAEGNQISQRLSSQEAFDYVTSAAGFEFDRTTVLGVMQ
                     YIAPYPVGTMVQLNTGEKGVVSQVYKGLTTRPTVKVLYDASGAEMAQPRDVALAAPEN
                     QTVLVAQTCEL"
     gene            14066..14608
                     /locus_tag="PLM74_01550"
     CDS             14066..14608
                     /locus_tag="PLM74_01550"
                     /inference="COORDINATES: protein motif:HMM:NF014660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HON41800.1"
                     /translation="MQVDSVRFGTLSVDDDKIIRFPKGLPGFEDEHRFFLMDHAASSK
                     IKWLHSADNPNLALVVADPFDLFEEYRPEVPDEIMRNLGITSSDGALVLTVLTVRAGQ
                     REGEPPAISANLLAPIVISRDRRIGTQVLLRSGEYGVRHQVPMLPGGDAKHEPSCGAG
                     GVGSAGSVGGVHGEAIVATA"
     gene            complement(14679..>15016)
                     /locus_tag="PLM74_01555"
     CDS             complement(14679..>15016)
                     /locus_tag="PLM74_01555"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HON41801.1"
                     /translation="AKMTFSLFRTPEAEKAVKAAATGRGRQLVLFGVEAHVCVLQSAL
                     DALALGYDVFVPADLCSSVKTADWDLAMRRMCQAGIVVTTAQAAAFELVGSADNPRFK
                     EAQPFLKQL"
BASE COUNT         3180 a         3974 c         5006 g         2856 t
ORIGIN      
        1 acagagaacc ccctcgacct cgtcattgaa ggcgacggtt tcttccaagt gcttctgccc
       61 gacggttcga ctgcgtacac ccgcacgggc gcattcaagg tggattctga ggggcggatt
      121 gtgacctctg acggattcgt catggagccc gaactggtcg tgccggccaa tgctgtcgac
      181 atatctatcg gcactgatgg cacggtgacg atccaagtgg caggcgagaa tgcccccact
      241 gagctcgggc agatccagct agctcgcttc ctgaacccgg gcgggttagt gtctatcgga
      301 cgcaacctct acaagccaag cgctgcgtcc ggcgatcccg cagtggggac tccgggactg
      361 gagggcattg gtaccatcgc tcagaagttc ctcgaaatgt ccaacgtgaa ggtagtagaa
      421 gagatggtca acatgatcat cgctcagaga gcctatgagg tgaactccaa ggccatccag
      481 acgtctgacg agatgctcgc tgccgccaat aacttacgga gataatgcag gcctgcatag
      541 agcggatggt gtggagtgag atgaggcgcg ctgcagtgtt tctttcgata agccgtgtag
      601 ccacgtgttg cgcggcggtg tgccgtgtgg tgttgtgctg tgtggtgcta tgctgcgcgg
      661 gactgtactg ggctgtgccg tgtcgggcgg aatcggaatc tgaaccaccg atcgacatga
      721 ccatcgagtt cgcaagcccg gcttacaccg ctggcccaca gatactcctg ggcgacatag
      781 cccgcgtcca cgtgctcgat gatgccctat gggccaggct cgcgcagctg gaggtgggcc
      841 cggcgccgat cccgggcggt tcgcgcaagg tcaatgtggg acaggtacaa gtaaggatga
      901 ggcaacagcg aatcgatccg gctcagttca gcttccaagg tcccgcggag atatcagtga
      961 ctgcagtggc tgagcgggtt gcggggagca gaatagagca ggcggcaatc gaagccgtgt
     1021 gcgctcatgc cggacatgca ctggagccgg aagatgtaaa ggtggtccga ttcgactatc
     1081 ctcagggcct gacagtacca cctggtccgg tcgttctgga ggcgtcaata gtaccgccca
     1141 gcgccgatgc ggcgctagtg agggccaatg taaaggtaat agtaggcggg actgccgcac
     1201 gcacggtgcc ggtatggttg gagatcaccg cgccgcctat ggtagcgcgg ggaaatagcg
     1261 tcctgctggt ggcggagggc cctggtatac acgttactgt gtcggccgta gcgctggaag
     1321 atggccgccc ggggcagcgc atccgcgtta ggaacgccgc ttccggtgtt gaggccgccg
     1381 gtcgggtcat gggtccgggt acggtgcagg tgactttgag tgccatgggg ggagtgctat
     1441 gaggagtatg aggatcagtg cgaggggcgc tgccgccgtc gccctgattg cgataaccac
     1501 tgccgcagtc gcggtggcta tgtgcccgac tgcagctacg gccacatcgt tgtacgcggc
     1561 aggcgcggcg cagtcgggct tttcagggat gtactgtgac tacaaagcca ggcaggtcgg
     1621 agacctgctt accataatgg tgacagaggt atcgtacgcg cagaacagaa cggatacgca
     1681 gactcaaaag ggcatagggg ccaatctcag cccagacggc gctgtgttcg atttcctcaa
     1741 agccgtcaag ctggacgcgg gcgcaggtct caacttgggc aattcgtcca agggcggaac
     1801 aacaacgtcg aggtcaggca agctaatcgg caccattacc gcccaggtag tcgaggtgct
     1861 gcccaacggc gacctgcgga tccgcggagt gcgcgaggtg tcggtcaatc gtgagaagga
     1921 gcagatgatt gtgacgggca ttgtgaggca acgtgacata tcgccaggca acaccatccc
     1981 gtctactatg gtggccgatg cgcagattgc gttctcgggc gatatctaca cgggcgagaa
     2041 gggcggtatc ttcaagactc tctgggacgg gttggtctac ttgtggaatt ggatcttcta
     2101 gcggcagcgg gggtggaagc atggccatca gtctagactg ggtcaaacaa gcaggagggc
     2161 ctctcaaaac ccgggccggg aaacccgggc ctacagacgg ttcgacctgg gagtcgtttt
     2221 cgattgtgtc agacaacgaa ctgcagcgcg cgccagggcg gttaaccagt caggcaattg
     2281 ccaacatcct ggcaatcttg ctggcgttgg tgctgatgat cttgactgcc ttgatgatgg
     2341 cctccggcag tgtgtgcgcg gccgagactg ggcccggagg ctcaggcgtg actgtgcgca
     2401 tcaaggacat tacgcgcatc tacggtgtac gcggaaatca gcttatcgga tatgggctgg
     2461 ttgtggggct cagaggtaca ggagatagcc gaagcgcccc gtacacagcg cgcagtatag
     2521 cgaacatgtt ggagaagttc atgatcacgg tagacccgtc ggaggtcaag tcgaagaaca
     2581 tcgcggcggt gatggttacg gccgtgctgc cgcctttcgc ggtgccaggc gataccctcg
     2641 acatcgtggc gtcctcaatc ggcgatgcaa agagcctact aggcggcaca ctgctactca
     2701 cggaactgtc cggattggac agtaaggtct acgcaatggc gcaggggcag atcattgcag
     2761 gcgcagatgc tgtgacgacg gtgggccgca ttccgtcagg cgccacagtc gaaggctatg
     2821 tccccatgga gatggtttcg ccagagggat acatggttct ttcgctgatc aacccggact
     2881 acaccactgc cgctcggatc gccgacgccg tgtgcgcccg gttcggaagc ggtagtgccg
     2941 tcgcgatcga cggcggctca gtgcgggtga ctgtgcccga aagcttccgt cagatgccaa
     3001 cgccgttcgc cgccgaagtc ggcgagctcc aggtgaagcc ggatgcgccg gcgcgggtga
     3061 tcgtcaactc ccgtacagga gtggtcgtag tcggcgccaa cgtcaagata agcccggccg
     3121 cggtgtcgta caacggcttc aatgtgcggg taggggcggc gaccgatgag gcagcggtag
     3181 cggcagcggc agcggccggg gctgcgggcg gagtcggcgt cggcgtcggc tctgcagccc
     3241 gacctgcggg tggagctgcg ggcggagctg caataggaag cacagtcctg ctagaagccg
     3301 gttccaccat ccagacgctg gtggcggcgc tcaatgcggt gggcgcgcgc cctcgggaca
     3361 ttatgagcat catggaggcc ttgaaggcct gcggcgctct tcaggcggag cttgagatca
     3421 tgtgaggggg cgtgcgaagt ggcgcagatg acggcggatg ttgtggtagt gcggaacatg
     3481 ggtaggagct tggccgatgt tggccgccgc ggcaaccccg gcggcatgag cagctcaggc
     3541 gtttccggcg gcgcgggaga ccgcgttgac gatccaaagc tgattcaggc ctgccgcgac
     3601 ttcgaggcga tattcataga aacagtcctc aagtcggcac gagccgggct gcccaaagat
     3661 gggctattct cctctcatga gcaggagatc gtaagcggca tgctcgatgc cgagatggct
     3721 cgcgctatgg cgcacggacg cggcatgggg ctcgccgagt tcatcctccg agacatcact
     3781 ggacgcgaga aacgggtgac gacgaatgcc cctagataac tgtgcaaggt gcgggaagct
     3841 gttcaacagg ctgtcacggc ccatctgccc ggactgcgcc agggaagagg agcgtcaggc
     3901 cgacgtggtc atggagttcc tcaaagataa ccccagcgcc actattgagg acatatccga
     3961 gtccaccggg gtggatagcc agatcatact gcgactgatc cgtgacaacc ggctgcgggc
     4021 caccggtgcc tactctgtgc ctcgcaactg caaggcatgt ggcgctccta tagacgcggg
     4081 tcagtactgc ggtcactgcc tcaggaagtt cggccaggct ttcgccggga aaggtatcgg
     4141 gggaggcgga ggtagcggcg atctccgcgg caggttgagc gatgctgacc gggccaggat
     4201 ggcgaggatg gcgacgcccg agcggaggcg cggcgacagg aaatcgtaag cgctgaacat
     4261 gccgcatgac atgtcgcgga atatgccgcg gaacacgccg cggaacatgc tctgacgcta
     4321 ccgggaagag ggtataaagt aaccgaaggg ctatgccgat aacaaggtta gaacttcggt
     4381 gaggtgatgg ccttgaaggt tacggggagt cggggatttg accccgtgaa gctgtactcc
     4441 cagaccacag gcaaggcgaa cagggctgac cggcagggcg aggccaggcc cgatgaggtg
     4501 tcgatatcag acgagggcaa agtgatgcac cacgccatgc aggccctgtc ctccattcct
     4561 gatgtgaggg aggagaaggt ccaatcggtc cgccagcgag ctgcggcggg cacttacgag
     4621 gcttccggca aagaggtagt caaaaagctc ttcagccggg ccgtacggcg gggtgacctg
     4681 ggtggaacac gcaacagaac tgatgagaat acttgacgag gagctgagcc tgtgctcaga
     4741 gctcctgtat gtgacgcggg cgcaaaagcc cgcgattatc tctggcgacg ttcttggcct
     4801 tgcggaactc gtggccaggg cagaagagac gatccggaga ttgcgagatg ttgagttatc
     4861 cctggcagat gtagcctctg agttcgcctt cgagtccagc ggcaagcccg ccgacgatct
     4921 ggattcggcc atagccactt tcgtggcgtc tctggacgac gcgcagagga ccgagtttca
     4981 gaagacgaag tccaggatcg cagggttgct ggacgacatc gcggcggcca atgctgtaaa
     5041 tgcgggactt cttcgcgatg cccttggcta catagacaac actgtcagac taatcgcagg
     5101 agctgacggc gacagctcta tctacagccg tctcggcatg ctcgacagga aagcctcgct
     5161 ggcagctgtc gacgagaccg cgtaacggct aggagggtgg agcacgtatg ctgaggtctt
     5221 ctttcaccgg ccttgagact gcccgtcggg cgctgttcgc acagcggatg gccattgaca
     5281 ccaccggaca caacatcgcc aacgccaaca cgcctggcta tcgaaggcag cgtgcggaga
     5341 tggtcgcaac gcctcccgct cccggcccga tgcagccagg gacgggggtc aaggtagcta
     5401 cgattgaacg catgcgcgac cacttcgtcg acatgcagat tcgccattcc cacgcgtcgc
     5461 tgggccgctg ggaggcgaga agcgattgct tagagcagtt gcaggaggta ttcaaggagc
     5521 ccagcgaaat cgggctttct accatgatgg accgtttctg gatcgcgtgg caggagctgt
     5581 cgggaggcgc gaccgatccg ggcaggcgca cggcggtcat tcaagaagct tccactctag
     5641 cctccgcctt cggcctgata tcaaggcagc ttaaggaggc gtccgacgat atccaggtta
     5701 acatcgactc gctcgtccac agtgtgaacg ccacagccga gagcatagcc cggctgaacc
     5761 acgagattgt gcgaggcggg gtagtgggcg agaacgtcag cgacctgatg gaccagcgcg
     5821 atatgctcct ggatcagctg gctcgggatg tcaacatcaa cgtagtcgaa actccggagg
     5881 gccaggtgaa cgtgtcggtg gggagcagac tgctcgtgtc cggcgtgacg gccttcaaga
     5941 tggatgtgag cggatcgttt gccggcgggc gcattcaggg cctgctggaa gcgaaaaacg
     6001 agataatccc gtcttaccag gcaaaactgg accagcttgc gcaggctctg aaagacacgg
     6061 tgaatgccca gcacagtgcg ggcgtcgaca tgtatggcga tgcgggccta ggtttcttca
     6121 cgggaaccgg cgcagctgat ctcgcggtaa gcgatgccct gaaaaacgat ccgaacaaga
     6181 tagctgccag ccggagcgga acagggccgg gcgatggcag caacgcgcta gccatggcgc
     6241 agctcaagga cgcgcttgcg attggaacgc ctccaactgc atcatttcga gatttctatg
     6301 ggagtatcat caccgagctg gggcttgcga ctcaggaatc gtccagggct gccgatgtgt
     6361 acaatggcgt tctcgagcag cagcagatga ggcgggacgc ggtgtcgggc gtttcgctcg
     6421 acgaagagtt gatgcagctg atcaggtatc aggcagcgta tgatgcggcg tccaggctgg
     6481 ttagcgttat ggatgagatg ctcgaaacgc tcatcaaccg taccgggatt gtcggcaggt
     6541 agccggatcg cagggagtgt tgtggaatgc gtgtgaccaa cagcaccatg atcaacaagg
     6601 caatacgcaa cgtggacggg gccttccgcc gctacatcgc gtgccaggaa aaggtggcct
     6661 cggggcgcag catcaaccat ctctcagacg atcctcatgg cgcggcaagg gctgtgcggc
     6721 tgcgctcggt tcttcgcggt gtggagcaat acaagcggaa tgcggagaac gcggatacgt
     6781 cggttcgcac gtacgacgac aacctcgaca aagtaggaag cattctcaac cgcgtgcgcg
     6841 agctggtggt gcggggcgca acggatagtg caactccaac agagcgcgag gccatggccg
     6901 tggaagtgcg tgaattattc gacgagcttg tgcatgcagg gaatgcatcg caccttgggg
     6961 cctacatgta cgcgggtcat cgcaccggca agagcccgtt cgaggtcatt gatgatatgg
     7021 gcgatgccaa ctggcccgtc agctacgccg gtgactccgg agtcaaccgg gtggaggttg
     7081 ggccgggcct tactctggag ataaacatca cgggcgagca ggtgtttatg gggcaaagcg
     7141 gtggcgtgaa catattcagg actgtgcgcc aaattgagca gcaccttacg gaaagcgatg
     7201 tgccgaagtt gtcgggctcg gaccttgcga gtctagacca agccataaac caggtgctga
     7261 agttccgggc cgagctgggc ggcaggggca gccgcctgga gctcgtcata aaccgcctgg
     7321 agagtgacag tgtctcaatt actgaacttc tcagcaagac tgaggatgtc gatctggctg
     7381 atgcgatcat gcgcctcgcc gagcaggacg tagtgtacca ggcagcactg gcggcagcgg
     7441 ccagatcggt gcaggccggg ctgcttcagt atctgaaata gaccgaaatc tggagggcgg
     7501 tgtgaatgga acaggcaatt gccggtgctg agatgcaatt ggtcgtgttc gagctgggcg
     7561 acgagtccta cggggtcgac atttcccggg tgcaggatat caacagaatg caggagataa
     7621 cggagatacc ccatgcgcct gagtcagtag tgggcgtgat caacctgcgg ggacgcgtga
     7681 tacccgtaat agacctgagg aagaggtttg gtcttcccga agccccgcac accaaggata
     7741 ccagaatcgt cgtagtgcac ctcgacgaca acctcatagg cgtgatcgtt gacgcagtct
     7801 cgcaggtact gcgaataccg gccgacatag tggaaccgcc gtctccggtg ctcgccggtg
     7861 tggattccag gtacctccgg ggaatcgcca agctcgatga gaagctcgtg atactgcttg
     7921 atctggactt tgttctgtcc cgccgtgagc aggaagcgat cagcgaggtc ttgtctgaag
     7981 aggtttaggg ggaagttcga tgcttcagtt tcccggcatg acagaggacg acataaaggt
     8041 attcctggct gaggccgatg agcaactgca gtacctcgaa gacgggctgc tcgacctgga
     8101 gcagagcagc gaagatcctg aggtgatcgc gcagatcttc cgcgcggcgc acacactcaa
     8161 ggggtcgtcc gctacgcttg ggcacgacaa gatggccaag ctcacgcact caatggagag
     8221 cgtcctcgac ttggttcgca aatcgcagat gaacgtgact ccggctctca tggatctgat
     8281 gttcgagtgc ctcgacatgc tgaggatact aaatcgagaa atagcaactg cgcaggatag
     8341 cggtgccgac atcaccgagc tcgacgatag actgctcgat gtgattgtga gcgttgagag
     8401 gcaggcgact tcaggcgaca ctatgggcgg agtcgcggcc ggagagaata cgccgtcgat
     8461 aggcggcgcc tccagtgaag acgcatggga agggcagttc accgggtccg gcgatgtgca
     8521 tgtcaaggtg agattcgcca agacatgccc catgccgtcg gtcagggcgt atcaggttat
     8581 cgacagacta tcgacactgg gtgtgttggt ctgctctaag cccagcgtgg agcggataga
     8641 ggccggagac gacgtgtttg atctagtggc tgtagtggaa tacgacggag atgctcttga
     8701 accccttatt gagacaattc gaagtgtgac cgatgtagag gacgtacgtg tgctgcaggt
     8761 gagcccggtc gggtcgactc gggcggcgat acaggccgat gcagctcccc aggccgcaga
     8821 cgcggccccg gcgaccgcgg ctgagactgt gactgcgaag ctcgcggttt cacgggaagg
     8881 tgcgaccggg ggcgcggcac gaaccgttgc gggagcctcg ccttctaggg cggcgactgc
     8941 ggccgccggc acgactacgg tgagggtgga cgtggccaga ctggacgctc tttcgaatct
     9001 cgtggcagag ctggttatcg acagaactca cctggctcag ctcgagagca ggctggcaga
     9061 gaagcatggc ggcgatgaac tggtgagcga gctcaacagg acatctactc acattggccg
     9121 ccttactacc gagcttcagg aggccatcaa caaggcacgg atgctgccta tcgacaatgt
     9181 gttcaaaagg tttccgcgta tggtgcgcga tcttgctcag aagcagggca aggaggtaga
     9241 gctcataatc catggtgagg acactgagct ggataggtcg gtgtcggagg agataggcga
     9301 ccccctgatt cacctgcttc gtaatgccat cggtcacggg atcgagcctc cggaggagcg
     9361 cgtcagagcc ggcaagccgc cgcgaggggt cgtccgcctt tcggctttcc gccaggagaa
     9421 ctacattgtt gtacaggtgg ccgacgacgg tcgcggcatc gatcccgaac tagtccgggc
     9481 atctgcgatc cgcaagggca tcattactga ggatgcagct gcaagactgt ctgacaagga
     9541 cgcgatcaac ctggtgtttt cgtcggggct gtctactgcg gctaaggctg acgacgtgtc
     9601 aggccgcggt gtggggatgg acatcgtccg caaaaacctg gaaaagctca atgggaacat
     9661 atccgtcgac acagcccttg gccgtggctc cacgttcacg gtgaaacttc cgctgacgct
     9721 ggcgataatc cgggcccttc tggtttcgct gggccagcgc atgtacgcga ttccgctcaa
     9781 ctcggtggag gagatcaccc gtgtggaaaa atcggatatc agcaccgttg ggggtagcga
     9841 agctatcagg cccaggggtg aggttgtgcc cctgctgtgg ttgggcgaag tgttcggcgt
     9901 gcgcgatccg ggcggctcgg cgcacctgtc tgtgccaggc caaaaggttt tcgcggtgat
     9961 tgcttcgtct catgaaggca aggtcggatt ggccgtcgac aggcttgtcg gagagatgga
    10021 aatagtcata aagagtctag gcacattcgt gggcaatata tcaggcgtgt cgggagtgac
    10081 catactaggc gacggacgag tggccctgat tgtggacgtg cccagcctgg tcaggagcgt
    10141 agtggatgaa cgaaacgctg ttagccagtg ctaacgtgat accggtcgga ataggagaac
    10201 tgcacgtgac cgacgtacaa gacatcgaga tggttgcata tggcctcggt tcgtgtgtag
    10261 gggttgttat ctacgatgag gcggccaggg caggggggat ggcccacgtg ttgctgccga
    10321 cgaggtcgtg gggatgcgga ctgcctgaca cgccggatgt gcacgcgcga tacgcggaca
    10381 cgggcgtaag agagcttcta gacagggtga ccgatctggg ggcgtcaagg cgccgactgg
    10441 tcgccaagat cgccgggggt gcaaggatgt tccaggtcgc tggcgacacg gatatgcttg
    10501 acatcggtgc gcgcaatgcg gaggcggtcc gggccgctct ggccgagacc ggcgtgccca
    10561 taatcgccga ggatgttggc ggcacggtag ggcgaaccat gagatacctt gtcggctcag
    10621 gcaaggttta cgtgcgggag ccgggcatgg cggagaagga gctgtagctc actgggggtg
    10681 tttggcaggt gccgactgtc cttgtagtgg acgatgcggc cttcatgcgt atgagggccg
    10741 ggaagctgct cgcgcaacat ggataccggg ttctcgaggc agaaaacggg ctcgatgcat
    10801 taaacatata catgcagcac aagccggatc tggtgctcat ggacataacc atgcccgtga
    10861 tggacggtat aacggcgctg aaggagatcc gtcaggcgga tccgcaagcc aaagtggtaa
    10921 tgttgtcggc catgggtcaa caggccatgg tgattgagtc cattaaggcg ggggccaaag
    10981 actttgttgt caagccctac gagcccgaca acgtgatggc ttccgtgagg aagttgatag
    11041 gctgatggcg cagatttccg ttttggtcgc cgacgactct gcattcatgc gcaaggtcgt
    11101 gtccgacctg atcgccgccg acccggagct tgtagtggtc ggcacggctc ggaacggctc
    11161 agactgcatc cagaaggccc gaagcatccg acctgatgtc atcaccctgg acgtggagat
    11221 gccggtgatg ggcgggctcg aagctcttcc aacactcacc acggagctgg gcatacccgt
    11281 cgtgatgctg tccagcctga cgcaggccgg ggcggatgtt accatccgag ccctcagcct
    11341 gggcgcagtt gacttcgtcc ccaaaccatc cggcgcaata tccctggaca tcgcggcagt
    11401 gcgcgatgaa cttgtgcgca aaatcaaggc cgctgccgca atatcggcgg gacttgtgcg
    11461 cacccttgct agagacttga gaacaccggc gctggggccg gctccggtat cgatgccgac
    11521 gacgccgacg acgccgacgc gaccgtcgac agcgcgggct gctgctcgca aggtggtcgt
    11581 gataggcacg tccacagggg ggcctagggc cctgacgcag gttattccgt ggttgccgcc
    11641 taacctgccg gcggccgtgc tgattgtgca gcatatgccg gccgggttca ccagatcgct
    11701 ggctgaacgt ttgaacgacc tgtctaggat cagggtgaca gaggctcagg gcggtgagac
    11761 cattgccgag ggcgaggctc tgatcgcagc cggcggacgg cacatgcttg tggcggccga
    11821 cggtgcgctg gttctggacg acagccctcc catgcacggt gtcaggcccg cggtggacat
    11881 gacgatgaac tccgctgttg acgttttcgg acgcaggatt gtcggcgtca tcatgaccgg
    11941 aatgggctct gacggagcgg acgccatggc gcgagtcaag ctcgctgggg gaaagaccat
    12001 agcggagcac gaatcgtcct gcgtcgtgta tgggatgccc aggtcggtgg tggaaagagg
    12061 catcgccgac aaagtggtcc ctctgccgga aatcgcggac gaaatcgtga acgctgtcct
    12121 ttcggtgtag gcggcaccag ccgaaaacgg gtgtggacgt agcagaggat gtaaggactg
    12181 gaccgtgagg tattgcctat ggattatgtc aacaccgcag attacgaggc ttttcgacgg
    12241 aaggtgattt cgcttaccgg aatagacctc ggtctttaca agtcgcagca gatgcagaga
    12301 cgcatccgaa gcctcctcga caaggtcgga actcctacct tcacgcacta cggtagtctt
    12361 cttgagcgtg atcctgaaga gttgagaagg ttctgcgatt acataatgat caacgtatcc
    12421 gagttcttcc ggaatcccga gaagtttgac gaactggaga aagagatact gccgtcactg
    12481 ctccggagga gccctaacct gagcatatgg agcgcgggcg cctccaatgg agcggagatc
    12541 tattcggtgg ccatgattct ggacgacata gcgcccaagg gatatcacaa gctcctggcc
    12601 acggatatcg attctggggc aattgcccag gccttagcag gagtgtatgg ccctcaagat
    12661 gtgaaaaatg tgtccagcgc gaggctggcg aggcacttca cttgcgaagg aagcaagtac
    12721 actatctcat cgaggatcaa gtccagggta cagtttagga tgcataacct gctcgccgac
    12781 aggttcgagc gaggttttga tctgattctc tgccgcaatg ttgtgattta ctttaccgac
    12841 gaggccaaac agacgctgta cgctaagttt agggagagct taagagacga cggcgttctc
    12901 ttcatcggag gcactgagac catcctcaat gcacgagaga ttgggttcct gcccacgtca
    12961 tcatttttct acaggcgagc gcctctcggg aaggaagctg ctgctcaatg attagggtgt
    13021 tatcggcaca agtaaggccg gggatgaagc ttgccgagca catatcaaac caggctggag
    13081 tagtgcagtt gcgtgcaggt acggtgctcg atgagaacgg tgcaagaagg ctgagtgaag
    13141 ttggtttcgg ctctatagct gtggaatacc ctggcactga cgacgtggtg gtgactcaca
    13201 ctgtgccccg gaagctgatg gaccacgggc gtgagctgct gcgcgcattt tacgaccatg
    13261 tgctgggcgc gggtgcgccc gagaacgtga gagtgccctt tggtgaactg ctggaactgg
    13321 tgcaggctgt ggaggagagc ctcgaattcg tgaatcagaa tgcaatagag gtatgtcctc
    13381 tgacgacggc ggatgagttc gattacgtag tcccgctgaa cgtggctgtg gtttcgatgt
    13441 tcgtggccgc gaaggcaggg ctggccaagc ggtcgcacga tgtggggctc ggcgcactac
    13501 tttgcaacat cggaacggcc cttacggacg atccagccag acatccggaa atgtcgctca
    13561 gagtgctccg acaggatcag cggttttcgg cctatagcaa ggcaatagtg ttccagcacg
    13621 aagagaggca tgacggcagc ggctatccca gagggcttgc aggagatgat atcgaaccac
    13681 tggcgagaat ggtggcagtt gccgatatgt actgcagcct gattgctgaa ggcaaccaga
    13741 tatcgcagag gctttcgtct caggaagcct tcgactatgt gactagcgca gctgggtttg
    13801 agttcgaccg caccacggtc ttgggcgtta tgcagtacat agctccctat ccggtgggca
    13861 cgatggtgca gctcaacacg ggtgagaagg gcgttgtgag ccaggtatac aagggcctga
    13921 cgaccagacc tacagtgaag gtgctgtatg acgccagcgg cgccgagatg gcacagccgc
    13981 gcgatgttgc gctggccgct ccggagaacc agacagtgct ggttgcgcag acttgtgagc
    14041 tctgatagtg gcgggagggt tcgatatgca ggttgatagt gtcaggttcg gcacactcag
    14101 tgtggacgac gacaagataa tacgatttcc caagggactt ccggggtttg aagacgagca
    14161 caggttcttt ctgatggatc atgcagcgag ttccaagatc aagtggctac acagtgcaga
    14221 caaccccaat ctagcccttg ttgtggccga cccgttcgac ctgttcgagg agtaccggcc
    14281 cgaagtgccg gacgagatca tgcggaatct tggaattacg agttccgatg gcgcgttggt
    14341 gctgacagtg ttaactgtga gagcagggca gcgcgagggc gaaccgcccg cgatcagcgc
    14401 taacttgctg gcgcccatcg taatctccag agatcgtaga ataggtaccc aagtgctgct
    14461 gaggtcggga gagtacggtg tacggcatca ggtgcccatg ctgccgggtg gggatgccaa
    14521 acatgagccc agttgcggtg caggtggcgt tggcagtgcg ggcagtgtcg gcggcgtcca
    14581 tggcgaggcg atagtggcga ctgcgtgacg gcgcgaccac gtgacggcgt gacggcatga
    14641 cgccgacagc cgagccatct gcgagcacag gtcgccgact acagctgctt gagaaacggc
    14701 tgcgcttcct tgaagcgagg attgtccgct gaaccgacga gctcgaacgc ggcggcctga
    14761 gcagtagtta cgacaatgcc cgcctggcac atcctgcgca tcgcaagatc ccagtcagca
    14821 gtcttcactg acgagcacag gtccgccggt acgaacacat cgtacccgag cgctaaggca
    14881 tcgagagcac tttgcagcac acacacgtgg gcttcgacgc caaacagcac aagctgcctg
    14941 ccgcgacccg ttgctgccgc ttttacggcc ttttcggcct cgggtgtgcg aaagaggctg
    15001 aaagtcatct tggcga
//
LOCUS       DAOTAC010000018        14934 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_3375_length_14934_cov_4.781034, whole genome shotgun sequence.
ACCESSION   DAOTAC010000018 DAOTAC010000000
VERSION     DAOTAC010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 14934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14934
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3375_length_14934_cov_4.781034"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..1089)
                     /gene="mraY"
                     /locus_tag="PLM74_01560"
     CDS             complement(121..1089)
                     /gene="mraY"
                     /locus_tag="PLM74_01560"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007953533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HON41802.1"
                     /translation="MSAVLARSLISTAVALLAAVVTAPPIIRSLARLKVGQVVRDDGP
                     SSHLAKTGTPSMGGVIMLIATLAGSLAAAGSHVRDLGWALFVSTGCGLVGMLDDYISI
                     VAHRPLGLRARQKLLFQLVLGLVLGAYGYVTPGLGSAIVIPFTRGAQLDLGIWYIPFV
                     ALVLVSATNAVNLTDGLDGLAGGTVAIASFAYVAIACRLGLPAVGVFAGALGGASLGF
                     VWWNAHPAAVFMGDTGSLALGAALASMAVMTKTELLLIVIGGVFVAETLSVAIQVISF
                     KLTRRRVFRMAPLHHHFELAGLAEPKIVVRFWIVAAFFAALGCAGL"
     gene            complement(1089..2513)
                     /gene="murF"
                     /locus_tag="PLM74_01565"
     CDS             complement(1089..2513)
                     /gene="murF"
                     /locus_tag="PLM74_01565"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HON41803.1"
                     /translation="MLDYTLAQVAIAVQGELAAGSADAQVASAAIDSRASGAGTLFFA
                     LPGERVDGHEFVAQASAAGASGAVVSKARPLDSFGDLPEAFGMIVVDDPARALARLAA
                     WHRRLMKASIVGVTGSAGKTTTKDMIAAVLREGFSVLATEGNMNNEIGLPLTLLRLEP
                     DHDVAVLEMAMRAPGEITALAKTARPDAGVVTNVGFAHIGLLGSQSGIADAKAELVRA
                     LPAHGFAVLNGDDSHVRAMERFAPGRSLLYGLSPDCSIRAVDVALSEDDTSFAVTLDG
                     QASPDVRFTVPVPGRHNVLNALAALAVGWRLGIDPGAMARGLASFRPSAMRMHFVQAP
                     RGFTVIDDTYNANPASVRCAIDAALQHGGQRRVIAVLGDMLELGDAGDAGHAELGAYA
                     VSAGVSGLVTVGPLAALAARSAVRAGLDPRRCHACDDNASAAHAAIEMASAGDVILVK
                     GSRGMRMEQIVSALADSPDSSGDR"
     gene            complement(2506..4053)
                     /locus_tag="PLM74_01570"
     CDS             complement(2506..4053)
                     /locus_tag="PLM74_01570"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006689962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HON41804.1"
                     /translation="MPEKRLDQLLAHLDAPEISGDAQVVITSLTYDSRKVAGAGTLFA
                     AFEGSRFDGHRFIGEAVRRGASAVLASRGRAVEVPEGITVVHVPDTRRALGAIACGFY
                     DDPSGQMTVTGVTGTKGKTTTTYIVAHILESVGIRTGLIGTVGYRTGRSVRPAPNTTP
                     EAADLQEIFYEMRQSGLDQAVMEVSSHAIALGRTALIDFDVAVFTNIGHDHLDFHRTV
                     DEYAEVKADLFRGLGRFGSRPGKPWPKSAVINADDPYSMKMIKASRRTGAHIVAFSAK
                     PGPLRDDCDDVATADDVNMGRSGTSFILRHAASGVRVPVRSRLVGAFNVSNAIAALAA
                     SYELGVSVEQGAAALASFPGVPGRFQLVDLGQEFTVIVDYAHSPDSLANVLATARDVT
                     RGRVICVFGCGGDRDRTKRPLMGGIAAEMADYSIVTSDNPRSEDPASIAREVSAGMGA
                     NNDRWEVILDRAEAIREAIGRARPGDLVLIAGKGHETYQIFADRTIDFDDVQVAQAAL
                     KELVPGA"
     gene            complement(4059..6188)
                     /locus_tag="PLM74_01575"
     CDS             complement(4059..6188)
                     /locus_tag="PLM74_01575"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HON41805.1"
                     /translation="MSVPHTARSASWISPSVIARKRIYTLFLLLTGVFAALVLRLLWL
                     QIVRGPELHARALEQRMRPVPVDPRRGTIYDRKMRELAVSISSDSVYAIPAEVRDPAS
                     VAHMLAPLLQMGEDTLLSRLTARQQTVWLKRKVEHDVAARIKELAAPGIGVAESGQRF
                     YPRDTLAAQVIGIAGIDNQGLEGLEQLYDSELRGVRGQIVAERDATGKEIPEGEHSYA
                     PARDGKSIVLTIDEVIQYICERELERAMADTRSEKGIILAMDPMTGEVLAMAMRPTFD
                     PNDYSRYPSSLRRNIAVCDMFEPGSTFKVVTAAAALEEGVVTPNTGFYDPGYIKVEDR
                     TIRCWKAGGHGSQSFTEAVENSCNPVFATLAVRLGSETFARYVRAFGLTDKTGIDFPG
                     EAVGMMHRASKMGPVELATSGFGQGISVTPLQLLNALCAVANGGTLMQPMLVREVIDE
                     SGASVRKIAPTRIRQVISERSARQLSTILQSVVVNGSGARAAVPGYAVAGKTGTAQKP
                     ERGGYGDKRIASFMGYAPANDPRIAVLVVLDEPKTDVKYGGVLAAPAFAAVIGDVMRY
                     MEIPPTYAARVPSQESTSGLITVPDLLGLALQDAMSSLKAAGLAGRAQGAGEIVTNQV
                     PKAGSRVAAGTTVVVYFGPEQLYNLEGDFVEVPDVTGKTLKEAAILLGARGLTVQPMG
                     RGVVARQQPLAGASARRGSAVTIFLEE"
     gene            complement(6216..6734)
                     /locus_tag="PLM74_01580"
     CDS             complement(6216..6734)
                     /locus_tag="PLM74_01580"
                     /inference="COORDINATES: protein motif:HMM:NF016860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HON41806.1"
                     /translation="MECSYARLGELGRDSARRRSSRTMRLMSGCGLVAVLAVALCVVV
                     LAERVAIVRASDSRDRARARAESLKNEWERMSLELARITSLDRVDQVARAELGMVDPP
                     RMGVVLVDARPMLSAANQPIPPSDTADVSQPPTVLANVAAVVEQMAVAAVSNLIAAWF
                     VVPTAQLPSILE"
     gene            complement(6737..7696)
                     /gene="rsmH"
                     /locus_tag="PLM74_01585"
     CDS             complement(6737..7696)
                     /gene="rsmH"
                     /locus_tag="PLM74_01585"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HON41807.1"
                     /translation="MDYSHQPVMVQEVMRYLAPAPGDVIVDCTLGGAGHSRVIAAAIG
                     PPGVLIGFDRDRQALQAASEALSDAPCRVSLVHGDYGDIESMLGKLGHPAPDGVLFDF
                     GVSSHQLDAVERGFTYREDAPLDMRMDQGIQLTAADIVNTASVRDLETIISDYGEERW
                     ARRIARHIAQERQKAPILTTERLVQAVVSAIPPGAGGREGPHPARRTFQALRIAVNDE
                     LGSVRRGTEGAVRAAACGARIVTLSYHSLEDRIVKELFRARERPCSCPPSLPACVCGR
                     RKSLEVITRKPVRPSEDEVQANPRARSAKLRAAIKVLGPGEDE"
     gene            complement(7763..8191)
                     /gene="mraZ"
                     /locus_tag="PLM74_01590"
     CDS             complement(7763..8191)
                     /gene="mraZ"
                     /locus_tag="PLM74_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HON41808.1"
                     /translation="MFIGEFRHSLDNKGRIIVPSRFREELDGRFILTRGLDTCLFGFT
                     ESEWRNQEEKLTTLPMTMADGRAFSRLFFSGASECELDRQGRALIPPHLRQYARIERD
                     IVVIGVSSRFEIWAQEVWEEYLSQVSGSYETIAEKLVSLG"
     gene            complement(8387..8920)
                     /locus_tag="PLM74_01595"
     CDS             complement(8387..8920)
                     /locus_tag="PLM74_01595"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HON41809.1"
                     /translation="MCDGDTVLLRQLAEHDLGTMARWDADHELTSLMGETVRTESESR
                     LRFAKLLSDRNSVAMAIVTQDGRLIGDIELTEIAWRSGDAELVVRIGDPSFRGKGLGS
                     CAVRAMLEAAFDRLNLARVYLRVCADNRRAIRCYLKCGFRREGVVRRKLGPSGEARQV
                     VLMTIMKSDFARVEIAS"
     gene            complement(9137..9397)
                     /locus_tag="PLM74_01600"
     CDS             complement(9137..9397)
                     /locus_tag="PLM74_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HON41810.1"
                     /translation="MEVLKVSAKSNPNSVAGALAGVIRERGGAEVQAIGAGAINQAVK
                     AVAIARGFVAPSGLDLVCVPAFVDVTIDGEERTAIKLIVQPR"
     gene            complement(9491..10282)
                     /locus_tag="PLM74_01605"
     CDS             complement(9491..10282)
                     /locus_tag="PLM74_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HON41811.1"
                     /translation="MKVAMFGDVIGRPGRVRALSVIGRLRAGGAVDFVVVNGENAAGG
                     IGLTPEVAREMLDGGVDVITSGNHVWNKREMAEYLDKEDRVLRPANYPSGAPGRGWTV
                     MSSGSLRLAVLNLQGRVFMQPIDCPFVRGRQALDEIGSSADITVVDFHAEATSEKQAL
                     GLYLDGGVGAVVGTHTHVQTADERVLPGGTAFITDLGMCGPIDSVIGMDPDTVIPRFI
                     TALPSRFAVAKGPVVVSGVIIEADHSTGRARGIERFIEVFYDSPR"
     gene            complement(10373..10903)
                     /locus_tag="PLM74_01610"
     CDS             complement(10373..10903)
                     /locus_tag="PLM74_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41812.1"
                     /translation="MREARNREGDGSSTSVGLLVSILVRYPEICTVSYTPDSRVLGFS
                     FMVARRVEADEFDAFRRLALASISVYLDLVRLEDAPVAEISSSYLGSVTQVQLTRDVA
                     TLTGDEISLLIGLILDAFGDDLATDRSEDLGEEELMVQEEFIANLLADLKETTQEKRL
                     IGFREEGRVVVFNRPA"
     gene            complement(10984..12507)
                     /gene="rny"
                     /locus_tag="PLM74_01615"
     CDS             complement(10984..12507)
                     /gene="rny"
                     /locus_tag="PLM74_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HON41813.1"
                     /translation="MLVYVLIAGIGVGGGYLARKWLAEAKIKSAESEARRLVEEAEKD
                     AEAQKREAVVEAKEEIHRMRSDFEKESRDRRVELQRSERRLLQREESLDRKTDQLEKK
                     ETVLERREHDIEKAHAEAEQLCQRQKQELERIGGLTSEQARQQLLREAEAEVQHDLAV
                     MIKECEAQAKDESDKRAREIITQAIQRCAADHVAEVTVSVVALPNDEMKGRIIGREGR
                     NIRALETLTGIDLIIDDTPEAVILSGFDPVRREVARVALERLIQDGRIHPARIEEMVE
                     RARKEVDAVVREEGERATFEVGVHGIHPELIKLLGRLRFRTSYGQNVLRHSIEVAQLA
                     GAMAAELGADVGLAKRGGLLHDIGKAVDHEVEGPHVAIGADIAKKYRESQAVINAVAS
                     HHGDVDPKTIEAVLVQAADAVSAARPGARRETLETYIKRLEKLEEIADSFDGVDKAYA
                     IQAGREIRIMVKPDRIDDTTSAKLARDIVKRIEQELEYPGQIKVTVIRETRAVDYAK"
     gene            complement(12735..13229)
                     /locus_tag="PLM74_01620"
     CDS             complement(12735..13229)
                     /locus_tag="PLM74_01620"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HON41814.1"
                     /translation="MPSSKRQHTLAEAYSAAYRLLAVQARTQSGLREALLRRGYNTEH
                     VDQVMAEMMRLGYIDDVAAASRWAEHAAGQRRYGVRGIATRLSERGIDPLLAAQAAQA
                     AYEAEGADEYQVALDLAAARVSSRELDDDELRERAVRRVARFLERRGFGADTISRVVR
                     NLTH"
     gene            complement(13216..14313)
                     /gene="recA"
                     /locus_tag="PLM74_01625"
     CDS             complement(13216..14313)
                     /gene="recA"
                     /locus_tag="PLM74_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018035133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HON41815.1"
                     /translation="MNERQKALEVAVTQIEKEFGKGSIMRLGESAGAAVVDVIPTGAI
                     ALDIALGVGGIPRGRVAEIYGPESSGKTTVALHIIAEAQKLGGVAAFIDAEHALDAAY
                     AKRLGVDIDNLLVSQPDTGEQALEITEALVRSGAVDVVVVDSVAALVPRSEIEGEMGD
                     SHVGLQARLMSQALRKLTGAISKSNTSVVFINQIREKVGVMFGNPETTPGGRALKFYA
                     SVRLEVRRVESLKQGSEQIGNRTRAKVVKNKVAPPFRDAEFDIIYGEGISREGSLLDV
                     GDATGVITKSGTWYSYGDLRLGQGKENSRQFLKSNPELAAEIEVKIREAVGLASRKAP
                     VEGDASAGTDGPDRSNSEDGAAKGRSGRAKQ"
     gene            complement(14339..>14934)
                     /gene="thpR"
                     /locus_tag="PLM74_01630"
     CDS             complement(14339..>14934)
                     /gene="thpR"
                     /locus_tag="PLM74_01630"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HON41816.1"
                     /translation="VEEYRRESGIPIIRTFIALPVAGSPVDAVSAMIRDMQQAGVGAK
                     WVEARNMHLTLKFLGDLPRTRIQPLADAVNHAVQGEPRFEIELRGAGAFPSPRNPRVF
                     WVGVREGAGRLTSLARSIDESTVARGFTPADKAFRPHLTIGRARGESADTRAADIISR
                     NSDTYWGTVLVDCIHVVASVLQPSGPIYTSLARIPLE"
BASE COUNT         2905 a         4986 c         4066 g         2977 t
ORIGIN      
        1 cccaggctga gcagcgcgag cgtgatgatg aatatcatga tgtcgggaag gcccttgtgc
       61 tcgctcatac tgcaggcgcc ggcgcgccgg cgcacctcct ctcacgacct agatcactgc
      121 ctagagcccg gcgcagccca gggcggcgaa gaacgccgcc acaatccaga acctcaccac
      181 gatcttgggc tctgccaatc ctgccagctc gaaatggtga tgaagcggcg ccatccggaa
      241 tacgcgcctt cgcgtcagct tgaatgagat cacctggata gccactgaga gggtctctgc
      301 aacaaacacg ccgccaatta cgatcaggag caactcagtc ttggtcatca ccgccatcga
      361 cgccaaagct gctcccagtg ccagcgaccc ggtatcgccc atgaacaccg ctgccggatg
      421 ggcattccac cagacaaagc ccagggacgc cccgcccagg gcgcccgcga acacgcccac
      481 cgccggtagg ccgagccggc acgcaatggc cacataggca aaggacgcaa tggctactgt
      541 gccgcctgcc agcccatcca acccgtctgt gaggttcacc gcattggtcg ccgacaccag
      601 caccagcgcc acgaagggta tgtaccatat cccaagatcc agttgagcgc ctcgcgtaaa
      661 cgggatgact atcgccgatc caagccccgg cgtgacgtag ccgtatgccc cgagcaccag
      721 cccgagaacc aactggaaca gcaatttctg gcgcgctctg aggcccagtg gacgatgggc
      781 tacgatagag atatagtcgt ccagcatccc gacaaggcca catccggtgc tgacgaacag
      841 agcccaaccc aggtcgcgca cgtgcgatcc ggctgcggct aggctcccgg ccagcgtggc
      901 aatgagcatg atgacaccgc ccatactggg cgtgcctgtc ttggccagat gagacgaagg
      961 cccgtcgtct ctgacgacct gcccgacttt gagacgggca agcgagcgta tgatgggcgg
     1021 cgccgtaact acagccgcca aaagcgctac ggcagttgat atgagcgaac gcgccagcac
     1081 tgcagacatt acctatcacc gctactatcc ggggaatcag cgagagccga cactatctgc
     1141 tccatgcgca ttcccctcga tccctttacc agtatcacat cgcccgcgct cgccatctca
     1201 atcgcggcgt gcgccgcaga ggcgttgtcg tcacacgcat ggcatcgacg cggatccagg
     1261 ccggctcgaa ccgcgcttcg cgcggccagc gcagccagtg gccccacggt gaccaggccc
     1321 gacaccccgg cggagacagc gtacgccccc agctccgcgt ggcctgcgtc accggcatcg
     1381 cccagctcca gcatgtctcc gaggaccgcg ataacgcggc gctgccctcc atgttgcagt
     1441 gctgcgtcga tagcgcacct cacagatgcc ggattggcat tgtaggtatc atctatcact
     1501 gtgaagccgc gtggcgcctg tacaaagtgc attctcatgg cggacggcct gaatgaggcc
     1561 agcccgcgtg ccatagcccc cggatcgatt cccaggcgcc agcctaccgc cagtgccgcc
     1621 agggcattca gcacgttgtg gcggccgggc acaggcaccg taaaccttac gtcgggagaa
     1681 gcctgcccgt ccaaggtcac ggcgaaggac gtgtcatctt ctgacagagc cacgtccacc
     1741 gcccggatgg agcaatcggg agaaagcccg tacagcagcg aacgcccggg cgcgaagcgc
     1801 tccatggccc gcacgtgcga gtcgtcccca ttgagcacgg cgaatccatg ggccggcaac
     1861 gccctgacga gctccgcctt ggcatcggct attccgcttt gcgagccgag caggcctatg
     1921 tgagcaaaac caacgttagt gaccacgccg gcgtccggcc ttgccgtttt cgcaagggca
     1981 gtgatctcac ccggggctcg catcgccatc tccagcaccg ctacgtcgtg gtctggctcc
     2041 agcctaagca gcgtcagcgg cagcccgatc tcgttgttca tgttcccttc tgtcgcgagc
     2101 accgaaaagc cctctcgaag caccgcagca atcatgtcct tggtcgtggt cttgccggcg
     2161 ctgcctgtta cccctactat gctggccttc attaggcgtc tgtgccaggc agccaggcga
     2221 gccagggccc gcgcaggatc atccaccacg atcatgccga acgcctcggg caggtcgcca
     2281 aatgagtcca atggccgggc tttcgaaact acagccccac tcgccccggc cgccgacgcc
     2341 tgggccacga actcgtggcc atcgacgcgc tcgcccggca gagcgaaaaa gagcgttccg
     2401 gcgccagaag ccctgctatc gatggcggcc gacgctacct gcgcatcggc cgaacccgcc
     2461 gccagctcgc cctggaccgc aatcgccact tgggcaagag tgtagtcaag cacccgggac
     2521 caactccttc aaagcagcct gcgccacttg cacgtcatcg aaatcgatgg tacgatcggc
     2581 gaaaatctga tatgtctcat ggcccttgcc cgctattagt accaagtcgc ccgggcgcgc
     2641 gcgcccgatg gcctcgcgga tagcctcggc tcggtccaag atcacttccc accgatcatt
     2701 gttcgccccc atccccgccg acacctcccg tgcaatggac gccgggtctt cgctgcgcgg
     2761 gttgtcggac gtgactatag agtagtcagc catctcagct gcgatcccgc ccatcagcgg
     2821 gcgcttcgtg cggtcacgat cgcctccgca gccgaatacg catatgactc ggccgcgcgt
     2881 gacgtcgcgt gcagtggcca gcacatttgc caaggagtcg ggagagtgcg catagtcgac
     2941 tatgaccgtg aactcctggc ctaggtcgac cagttggaac ctgcctggga ctccggggaa
     3001 actagccagt gctgccgcgc cctgctccac tgatacgccc agctcatacg aggcagccag
     3061 agcagcaatg gcgttcgaga cgttgaacgc gcctacaagg cgcgacctga ccgggacgcg
     3121 cacgcctgac gcggcgtgtc gcaagatgaa ggatgtcccg ctccgtccca tattcacatc
     3181 gtcagccgtg gccacatcgt cacagtcgtc tcgcaagggc ccgggcttgg ccgagaatgc
     3241 cactatgtgg gctccagtcc ttcgagatgc cttgatcatc ttcattgagt acggatcgtc
     3301 ggcgttgatc accgcggact tgggccatgg cttacccggc cttgagccga accggccgag
     3361 ccctcggaag agatcggctt tgacctcagc atactcgtcg acagtccggt ggaagtcgag
     3421 atggtcatgg cctatgttgg tgaaaactgc cacatcgaag tcaatcagcg cggtgcgtcc
     3481 gagcgcaatg gcgtgcgacg acacctccat cactgcctga tccagtccgc tctggcgcat
     3541 ctcatagaaa atctcctgca agtcggcggc ctcaggcgtg gtgttgggcg caggacgcac
     3601 agaacgcccg gttctgtacc caactgtgcc gatgagccct gtgcgtatgc ccacggattc
     3661 gaggatatgg gcaacgatgt acgtggtagt ggtcttgccc ttggtgccgg tcacgccggt
     3721 cactgtcatc tgccccgacg ggtcgtcgta gaacccgcag gcgatggcgc ccagtgcacg
     3781 acgggtgtca ggcacatgaa ccaccgtgat tccttcgggc acttccacgg cgcgcccacg
     3841 cgaggcaagc accgcgctcg cgccgcgtct aaccgcctcg ccgataaagc gatgcccatc
     3901 aaaacgcgac ccttcaaagg ctgcgaacag ggtgccggcg ccggccactt tgcgagagtc
     3961 gtatgtaagg ctcgtgatca cgacctgcgc atctcccgag atctcaggtg cgtcaaggtg
     4021 cgcgagcaac tgatcaaggc gcttttcagg catactgctc attcctcgag gaatattgtg
     4081 acggccgagc cccgtcttgc cgaggcgccg gccagaggct gttgccgggc cacaacgccg
     4141 cggcccatgg gctgcactgt cagccctctg gccccaagca ggattgcggc ctccttaaga
     4201 gtcttcccag tcacgtccgg cacttccaca aaatcgccct caagattata tagctgctca
     4261 ggaccaaagt aaacgacgac ggtcgtaccg gccgcaacgc gcgagcccgc cttaggcacc
     4321 tggttggtca cgatctcgcc tgcgccctga gctctgcctg ccagcccggc agccttaagt
     4381 gacgacatgg cgtcctgcag ggccagcccg agcaggtccg gcacagttat caggccggaa
     4441 gtcgactcct gtgacggcac tctcgcggca tatgtcggag gaatctccat gtagcgcatc
     4501 acgtccccta tcaccgccgc gaaggcgggc gcagctagga cgccgccgta cttcacatca
     4561 gtcttgggct catccaacac gaccagcacg gcaatgcgcg gatcattcgc cggagcatat
     4621 cccatgaacg atgcaatacg cttgtcgccg taccctccgc gctcgggttt ctgcgcggtc
     4681 cctgtcttgc cggcaactgc gtatccggga accgctgcgc gggcgccgga cccgttaacc
     4741 accacagact ggagtatcgt tgagagttgg cgagcgcttc gctctgatat aacctgccgg
     4801 atccgggttg gcgcgatttt acgcacggag gcgccggact catcaatgac ctcgcggacc
     4861 agcatcggct gcataagcgt gcccccgttc gccacagcgc acagggcgtt cagaagctgc
     4921 agaggagtga ctgagattcc ctgcccgaac ccgctcgtag ccagctccac cggacccatc
     4981 ttggacgctc ggtgcatcat gccgacggcc tcgcccggga agtcgatgcc ggtcttgtca
     5041 gtgagaccga acgcgcgcac gtagcgggcg aaagtctccg acccaagcct gacggcaagg
     5101 gtggcaaaca cggggttgca cgaattctcc acagcctcgg tgaagctctg ggatccgtga
     5161 ccccctgctt tccagcaccg tatcgtcctg tcctcaacct tgatgtaacc ggggtcgtag
     5221 aatccagtgt tgggagtgac cacgccttct tcaagcgcag cagcggcagt cactaccttg
     5281 aacgtggatc cgggctcgaa catgtcgcac acggcaatat tccggcgcag gcttgaaggg
     5341 tagcgactgt agtcattggg gtcgaaagtc ggacgcatgg ccatggccag gacctctcct
     5401 gtcattggat ccatagccag gataatgcct ttctcggacc gcgtgtcggc catggcgcgt
     5461 tctagttcgc gctcgcatat gtactggatc acttcatcga tggtaaggac tatgctcttt
     5521 ccatccctgg caggggcgta agagtgctca ccttcaggta tctccttgcc ggtggcgtcg
     5581 cgctcagcaa ctatctggcc gcgtactccc ctgagttcgg aatcgtatag ctgttccaag
     5641 ccctcaaggc cctggttatc gattcccgca atgccgatca cctgtgcggc caacgtatcc
     5701 cggggataga aacgttggcc gctctccgct acgcctattc ccggagctgc cagttccttt
     5761 atgcgcgccg ccacatcatg ctcgaccttg cgtttgagcc atacggtctg ctgccgagcg
     5821 gtaagccttg aaagaagcgt atcctcgccc atctgcagca gtggagcgag catgtgcgca
     5881 acggacgcgg gatcgcgaac ttcggccgga atggcataca ccgaatcgga gctgatgctt
     5941 acggccagct ccctcatctt cctgtcgtag atcgtgccac gcctcgggtc gactggaaca
     6001 ggccgcattc tctgttccaa agcgcgggca tggagctcag gtcccctgac gatctgcagc
     6061 cagagaagac gaagtaccag cgcagcaaaa acgcccgtca gcaggagaaa gagtgtgtag
     6121 attctctttc tcgcgatgac cgacggggaa atccaggatg ctgatcgagc cgtatgtgga
     6181 accgacacta caggagcccc cacatcttgc ttggttcatt caagtatcga cggcagctgg
     6241 gcagtgggaa cgacaaacca cgcggcaatg aggttcgata ctgccgctac cgccatctgc
     6301 tcgacaacgg cagccacgtt cgcgagcaca gtgggaggtt gtgatacatc cgcagtatcg
     6361 cttggcggaa tcggctggtt agcagcgctc agcatgggcc gtgcatcgac caagacgact
     6421 cccatcctag gcggatccac cattcccagt tcagcccggg ccacctgatc tactctgtca
     6481 agagatgtga tccgcgcgag ctccaacgac atccgctccc actcattttt gagcgactca
     6541 gcccgcgccc gggctctgtc gcggctgtcg cttgcgcgca ctatcgcaac tctctccgca
     6601 agaacgacaa cgcagagagc aacagccagc actgctacga gcccgcaccc agacatgagc
     6661 cgcatggttc gcgacgatct gcgcctggcc gaatcacgcc ccagctcgcc tagtctagcg
     6721 taactgcact ccatcactat tcatcctccc ccgggcctag aactttgatt gcagccctga
     6781 gcttggcgct tcgcgctctg ggattggctt gcacttcgtc ttccgacgga cgaaccggct
     6841 tgcgcgtgat cacctcgaga gacttgcggc gaccgcagac gcacgccgga agagacggcg
     6901 ggcatgagca cgggcgctct ctggctcgaa acagctcttt tacgatccgg tcctccaggg
     6961 agtggtatga cagcgtcact atgcgcgccc cacaggcagc cgctcgaacg gctccttctg
     7021 ttccccttcg aaccgagccc aactcgtcgt tgacggctat tctgagcgcc tggaacgttc
     7081 tgcgggccgg atgtgggccc tcgcgtcctc ctgctccagg cggaatcgcc gaaaccaccg
     7141 cctgcacaag gcgttcggtg gtcagtattg gcgccttctg tcgctcctgg gcgatatggc
     7201 gggcaatccg ccgcgcccat ctttcctcgc cgtagtctga gatgatcgtc tccaagtctc
     7261 ggaccgacgc cgtgttgact atgtcggccg cggtcaactg aattccctgg tccatccgca
     7321 tatcgagtgg agcgtcctcc ctgtaggtga agccgcgttc aacagcgtcc agctgatgag
     7381 aactgactcc gaaatcaaac agcacgccgt ccggtgcggg atgcccgagt ttcccgagca
     7441 tagactctat gtcgccatag tcgccatgca ccaggctcac ccggcagggc gcgtcggaga
     7501 gcgcctccga ggccgcttgc agcgcttgcc gatcacgatc aaagccgatg agcacacccg
     7561 gcgggccgat tgccgcagca atcaccctgg aatgtcctgc gccgcccaat gtgcagtcaa
     7621 cgatcacatc gccgggagcc ggcgccaggt atctcatgac ctcctgaacc attaccggtt
     7681 ggtggctgta gtccatcagt caactcccgc tcgctgagct ctcgccgccg cggcgacctt
     7741 gggcatcctg aagacgccat gattacccca gcgatacgag cttttctgca atcgtttcgt
     7801 aggaacccga tacctgcgat aggtactctt cccacacttc ctgcgcccat atctcgaatc
     7861 tcgatgagac accgattact acgatatccc gttctatccg ggcatattgc cgcagatggg
     7921 gcgggatcag agctcttccc tgacggtcaa gctcgcactc gcttgcgccg gagaagaaca
     7981 acctggagaa agcgcgtcca tcagccattg tcatagggag cgtagtcagc ttctcttcct
     8041 gattgcgcca ttcggattcg gtgaacccga acaggcacgt atcgaggccc cgagtcagga
     8101 tgaacctgcc atcgagttcc tcgcggaatc tggagggcac tatgatccga cctttgttgt
     8161 ccagagagtg tctgaactcc cctatgaaca ccgtgaccca ccactttact ccactctgct
     8221 ccacttgcac tatcatttcg aggcccgaac gctttctcct gcaacgttgc acaaatattt
     8281 ggccatcatt gccactctgg cgaaaccggg caaaaaaaga agagggcttt agccctcttc
     8341 ggatcgagcg tcggatggaa agccgtgcgg ggcgccctcc gcctgcctag gacgcaatct
     8401 ccactctggc aaagtccgac ttcatgatgg tcatcaacac aacctggcgc gcctcgccgc
     8461 ttgggcccag cttccgccgg accactccct ccctacggaa cccgcactta aggtagcatc
     8521 ggatcgccct gcggttatca gcacagactc tgagatatac gcgggcaagg ttcagccgat
     8581 cgaatgccgc ctccagcatg gcgcggacgg cgcacgagcc caggcccttc ccccggaacg
     8641 aggggtcgcc tattcgcacc actagttccg cgtctccgct gcgccacgca atctctgtca
     8701 gctcaatatc ccctatgagc cggccgtcct gcgtgacaat ggccatcgcc acgctgttcc
     8761 tatcgctgag caacttggcg aaccgaagcc gcgattcgct ctcggttctg acggtctccc
     8821 ccatcaggct cgtcagctcg tgatccgcat cccacctcgc catcgtgcca agatcgtgct
     8881 ccgcgagctg gcggagcagt acagtgtccc cgtcgcacat agtaaccctt cctctgctac
     8941 gactccacct cccaaggtgg gttgtggatt cgcccttgag gagcggttta cctccaagaa
     9001 actgtatcca gagtcggttc tacacgcctg ttgccccgcg cagagctgat taatagcagg
     9061 tcacattatg ccgagtgccg ggcacaagcc cggcacgacg gtcaatggct gatgatttcc
     9121 gattggatta tgacggctag cggggttgaa cgatcagctt gatcgcggtg cgctcctcgc
     9181 cgtcgattgt gacatcgacg aacgctggaa cgcatacaag gtcgaggccg cttggcgcca
     9241 cgaagccccg cgcgatcgcc acagccttga ccgcctggtt gatagctccg gcgcctatgg
     9301 cctgcacctc agcgccgccg cgttcgcgga taacccctgc caaagctccg gctaccgagt
     9361 ttggattcga ctttgcagaa acctttagga cctccatgct cttatcctcc tgccggctga
     9421 tcgtattgac gttccaaagt agtgtattag tcctctattg gaaaactcct tctactgttt
     9481 ggaacgggac tcaccgaggc gaatcgtaaa atacctcgat aaaccgttct atccctcgcg
     9541 ccctgccagt ggaatgatct gcttcgatga tgacccctga caccacgacc ggaccctttg
     9601 caacggcgaa acgcgacggc agcgccgtga tgaaccgcgg aattaccgtg tctgggtcca
     9661 ttcctatgac agagtcgatt ggcccgcaca tgccgagatc agtgatgaaa gcagtgcctc
     9721 ccgggagcac tctctcatcg gcggtttgga catgcgtgtg cgtgccgacc accgcaccca
     9781 caccgccatc caggtaaagc ccgagcgcct gtttttccga agtcgcctcc gcgtggaagt
     9841 ccacgacagt tatatcggca gaagacccga tttcgtccag tgcctgcctg ccccggacga
     9901 acggacagtc aatgggttgc atgaacactc gcccctgcag gttgaggact gccaggcgca
     9961 gactcccaga ggacatgact gtccaacccc tgcccggcgc tcccgatggg taattggccg
    10021 ggcgcagcac ccgatcctcc ttatcgaggt attcagccat ctcccgcttg ttccacacgt
    10081 ggttcccgga tgtgatcaca tccacgccac cgtcaagcat ctcgcgcgcc acttcaggcg
    10141 tgagaccgat tcctcctgca gcgttctcgc catttaccac aacgaagtcc actgccccgc
    10201 cggctctgag ccggcctatg accgacaacg cacgtacacg tcccggcctt ccgataacgt
    10261 cgccgaacat tgcgactttc aagcatattc ctccctgagg gcaagcgcgg gcaggcctaa
    10321 ttatcagcct gcccaccttt ctccatcata ttcgcaaacc aggcccacgc gatcaggcgg
    10381 gcctgttaaa caccacgacc ctgccctctt ctcggaagcc tatgaggcgc ttctcctgag
    10441 tagtctcctt gagatccgcc aggaggttgg cgatgaactc ctcctgcacc atcagctctt
    10501 cctcccctag atcctcagac ctgtcagtcg ccaggtcgtc tccgaaggca tcaagaatca
    10561 gcccgataag cagagatatc tcgtcgccgg tcagtgtggc tacatcgcgg gtgagctgaa
    10621 cctgtgttac gcttcccagg taggaggagc tgatctcggc tacaggcgcg tcttcgagcc
    10681 ttacaaggtc gagatagact gagatgctcg caagagccag gcgtctgaag gcatcgaact
    10741 catcggcctc cactctcctt gcaaccatga acgagaatcc gagaaccctc gaatccggcg
    10801 tgtacgacac ggtacagatc tccggatatc gcaccagaat cgacactagc aggccgacac
    10861 tggtcgaaga accatcgcct tctctgttgc gcgcctctct cacctctgcc accttcttcc
    10921 cccgatcatc ggcccggatt cctgctgatc attctacgca tggcgcggac cttgggctac
    10981 ctgtcacttg gcgtagtcca cggctctcgt ttcgcgaatg acagtcactt tgatctggcc
    11041 cgggtattcc aactcctgct ctatacgctt gacaatgtcg cgtgccaatt tagcggatgt
    11101 tgtgtcgtca atccggtcgg gcttgaccat gattcgaatc tcgcgaccgg cctggatggc
    11161 ataagccttg tcaacgccat cgaaggaatc ggctatctct tccagtttct cgagacgctt
    11221 gatgtaagtc tccagagtct cacggcgcgc cccggggcgc gcagcggaca ccgcatctgc
    11281 cgcctgcacc aggacggctt cgatggtctt gggatccaca tcgccatggt gcgacgcgac
    11341 agcgtttatc accgcctgcg actcgcgata cttcttggcg atgtcggcgc cgatggcgac
    11401 gtgaggacct tccacctcgt ggtcgactgc cttgcctata tcgtgcagca gtcctcctcg
    11461 cttagcaagg cctacgtccg cgccaagttc ggcagccata gcgcccgcca gttgagcgac
    11521 ttcaatagag tgcctgagca cattctgacc atagctcgta cggaatctga gcctgccgag
    11581 gagcttaatc agctccggat ggatgccgtg tacgcctact tcgaaagtgg ccctttcgcc
    11641 ttcttcgcga actacagcat ccacttcctt gcgggcgcgc tcaaccatct cttcaatcct
    11701 cgccggatgt atcctgccat cttggatcag tcgttccagc gcgacgcggg ctacctcccg
    11761 cctgaccggg tcgaaacctg ataggataac cgcttcaggc gtatcgtcga tgatcagatc
    11821 gattccagtg agggtctcta gggcgcgtat gtttcggccc tctcggccga tgatgcggcc
    11881 cttcatctca tcgttgggaa gcgccaccac cgacacagtg acttcagcca catgatcggc
    11941 agcgcacctc tgtatcgcct gggttatgat ctctcgcgca cgcttgtcgg attcatcctt
    12001 agcctgggct tcgcactcct ttatcatgac cgccaggtca tgttggacct cagcttcagc
    12061 ttctctcagc aactgctgac gcgcctgctc ggacgtgagt cctccgatcc tctccagttc
    12121 ctgcttctgc ctctgacaca attgctccgc ctcagcatgc gccttttcga tgtcgtgctc
    12181 acgccgctca aggactgttt ccttcttctc aagctgatcg gtctttctgt caagcgattc
    12241 ttccctctgg agcagccgtc tttccgaacg ctgtagttca acccgcctgt cgcgggattc
    12301 tttttcgaag tcactccgca tcctgtgaat ttcctctttg gcctccacga ccgcctcgcg
    12361 cttttgagct tcggcgtcct tttcggcctc ctctactaag cgccgtgcct cgctttccgc
    12421 agactttatc ttcgcttcgg ccagccactt gcgggccaga tatcctccac cgactccgat
    12481 gccggctatc aacacataga caagcatctg tatgaatggg ctaatgctga tccacctccc
    12541 ttagcacaaa caatcgcgta cataatcaac aagctcgatt acagtacaag gaaagccgag
    12601 cgccaactcg gcttgaacgg aacacgcggc atggtgttca cgggccggaa accccagccc
    12661 cagactagat tctatatgta gtgcccaagc actgtcaagc tggcccagcc cagcctgccc
    12721 agacgtcggg cccttcagtg agtgagattt ctcaccacac gggatattgt gtcggcgccg
    12781 aaccctctcc gttcaaggaa ccgggcgacg cgccttaccg cccgttccct cagctcatcg
    12841 tcgtctagct cacgtgaaga aacccgggca gcggccagat ccagcgctac ctggtattca
    12901 tccgcgccct ccgcctcgta ggccgcctgc gctgcctgcg cagcaagcag cgggtcgatc
    12961 ccgcgctccg agagcctcgt ggcgatgcct cgcacgccat accgacgctg gccggcggca
    13021 tgctctgccc agcgagatgc cgccgcgaca tcgtcgatat agcccagacg catcatctcc
    13081 gccatgacct ggtcaacatg ctcagtgttg tagccccgcc tgagcagggc ctcccggagc
    13141 ccggactgtg tgcgagcctg gacagccagg agcctgtacg cagctgagta tgcctcggcc
    13201 agagtgtgtt ggcgtttact gcttggcacg tccgctgcgc ccctttgcgg cgccgtcctc
    13261 cgagttggat cgatcggggc cgtccgtacc ggccgaggcg tcgccttcga ccggggcctt
    13321 gcggctcgcc aacccaaccg cctccctgat cttgacttcg atttccgcgg ccagttcagg
    13381 gttggacttg aggaactggc gcgagttctc cttgccctgc cccaacctga gatcaccgta
    13441 tgagtaccaa gtgccgctct tagttatgac tccggttgca tcgcccacat ccaacaaaga
    13501 cccttcccgg gatatgcctt caccgtatat gatgtcgaac tcagcatccc tgaagggtgg
    13561 cgccaccttg ttcttgacca ccttggcacg ggtgcggttg cctatctgct cagaaccttg
    13621 cttgagcgat tccacccgac gcacctccag tcgcacggac gcatagaact tgagcgcccg
    13681 gcctcctggg gtcgtttcgg ggttcccgaa catcacgcct accttctcgc gaatctggtt
    13741 aataaacacg acgctcgtat tagacttcga gatagcgccg gtcaatttgc gcagggcctg
    13801 tgacatcagc ctggcctgca ggcccacgtg ggagtcgccc atctcgccct caatttccga
    13861 gcgcggcaca agcgcagcga ccgaatccac aacaacaaca tccactgcgc cgcttcggac
    13921 taaggcttca gtgatctcga gggcctgctc tccagtgtcg ggctgcgaaa ccagcagatt
    13981 atcgatgtcg actccaaggc gcttagcata cgctgcgtcc agagcgtgtt cggcatcgat
    14041 gaaggccgct acgccaccta gtttctgtgc ctctgcgatg atatgcaatg ccactgtggt
    14101 cttgccagac gattccggcc cgtatatctc tgcgacccga ccgcggggta tcccgcccac
    14161 gcccagcgct atgtcgagcg cgatcgcgcc ggtcgggatc acatccacca cagcagcgcc
    14221 cgccgactcg cccaggcgca ttatggaacc ttttccaaac tctttttcga tctgagtaac
    14281 cgccacttcc agcgccttct gtctttcgtt caagaccgat cgctccttac tatgctggtc
    14341 actccagcgg aatacgggca agtgatgtgt aaatggggcc tgacggctgg agtacactgg
    14401 ccaccacatg tatgcaatct accaagaccg tcccccaata cgtatcggaa tttcgcgaga
    14461 tgatgtcggc tgcccgtgtg tccgccgact ccccgcgggc tcttcctatg gtgaggtgag
    14521 ggcggaacgc cttatcggcc ggagtgaaac ctcgagctac cgtcgactca tcgatggagc
    14581 gtgcaaggct cgtcaagcgg cccgcgcctt cgcgtacccc cacccagaat acacggggat
    14641 tccgaggact ggggaaagcg cccgcgccgc gaagctcgat ctcgaacctg ggttcgccct
    14701 gcacagcatg gttcactgca tcggccaacg gctgtatacg tgtgcgcggc agatcgccca
    14761 ggaacttcag ggtaagatgc atgttgcgag cctcaaccca cttggcgccc actccggctt
    14821 gttgcatgtc gcgaatcatc gcgcttaccg catccaccgg cgaccctgct accggcagag
    14881 cgatgaacgt tctgataatt ggaattccag actcgcgtcg atattcctcc acaa
//
LOCUS       DAOTAC010000019        14775 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_3423_length_14775_cov_5.045516, whole genome shotgun sequence.
ACCESSION   DAOTAC010000019 DAOTAC010000000
VERSION     DAOTAC010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 14775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14775
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3423_length_14775_cov_5.045516"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="PLM74_01635"
     CDS             <1..194
                     /locus_tag="PLM74_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the isomerization of sedoheptulose
                     7-phosphate to D-glycero-D-manno-heptose 7-phosphate;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HON41817.1"
                     /translation="AIVGRAMGIRVIGLVGRNGGALAPICDTAIRVPSVSTAEIQQMH
                     VSVYHHVCREVERRLAAQA"
     gene            878..1816
                     /locus_tag="PLM74_01640"
     CDS             878..1816
                     /locus_tag="PLM74_01640"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HON41818.1"
                     /translation="MQRSVATANPKYGILMALTAAIVWGTLGVFAKAIYKYHLDPLVL
                     VTMRAGIAFVALLAIMLFRSPGLLKVERRDIIDFAYVGVVGIALEYWVYFETLKRTSS
                     SVASILLYTAPVFVTAGAAAFFGEKITRRGLAALGVVLIGGFLVAGGLEARVLTQDRV
                     GVLFGIASALTYAGYTLLSKRTLPKYGSWTTVLYAFGFGTILLALMSGPRLAQLATVP
                     FEGWMLMLGLALGPTLLSYGLYVKSLEYIEASRATVVCMMEPVTASILGFLLLGERLA
                     GWQLVGALLVLGGVGVLNLRASPPLPGDVASVEGSH"
     gene            2234..2659
                     /locus_tag="PLM74_01645"
     CDS             2234..2659
                     /locus_tag="PLM74_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41819.1"
                     /translation="MAQDATHLILARPDSGSIVHEVLHSLFAQALECVRPAVDEGNAL
                     YAPVRDSMIRMGYAWDDSPASWRRVWEESLVRATAIWAECGDCPAEVARAAAGHASMG
                     FIYVPKLLQCFAGSCSGLNDVAGFILECLKVCAGTVLHP"
     gene            2960..3592
                     /locus_tag="PLM74_01650"
     CDS             2960..3592
                     /locus_tag="PLM74_01650"
                     /inference="COORDINATES: protein motif:HMM:NF040376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6305 family protein"
                     /protein_id="HON41820.1"
                     /translation="MISSNRRIQITLGLIAVLMAGLASAAPTAPQGIDMPSKAPTLKA
                     PVAVTTIGQAPSAMMVRLLLQKAKIQVVQKDLLTPQDIAEAAKNPNAAFKTLIMTMGT
                     SLKGMGGAGVNVDSEVTRCNALVAEAKKHGIVVVGVQIEGAARRSDESDEKSIRAVAP
                     QSDVLIIRREVDNDNYFTNMAKKNGVPIIRAKEAADFGYVFGTLFGSPAK"
     gene            3615..4901
                     /locus_tag="PLM74_01655"
     CDS             3615..4901
                     /locus_tag="PLM74_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter"
                     /protein_id="HON41821.1"
                     /translation="MFLHAAIVLAVMAAAFAVSIALHAGSEIAMFAAALAGALAHGAG
                     VPARHIVEGAFTYFDVILIFITATFFMNLLKEAGGAAHIVRGIISKFSRNRFMLLLLL
                     TLIMLVPGALTGSGTVTVLVVGAFVGSVLSYMGISKDRAAAIVFLCASMSAAAPPINL
                     WAMMAAAGANMPYVGFTIPLAVISIAGALFSMFYLGWRGRDVDAARALREIPEPPHGM
                     NWTKVLVPFLIFFGVVVAGRVWPLTAPVVGLPLAFALAGAAVVLLSPVKLDLFGILSK
                     TIDDLLPLLSTVTIVGMLVQIMALSGARGLISLSVVTLPIAVLFATLFLVLPISEGIF
                     QFAAAPLLGVPLVFLFNMKGYNPVISLSGMAAMWPLGDCLPPTAPVGRAAVMATGYDG
                     DYWTFLRRCIVPAVFIMAIGTMYLVYSHKLGFLARG"
     gene            5015..5179
                     /locus_tag="PLM74_01660"
     CDS             5015..5179
                     /locus_tag="PLM74_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41822.1"
                     /translation="MEAVQMVIGVLYYFMTAVIALVIGASFFRQRDLRKMILYAAVLI
                     PFVLRALRVK"
     gene            5206..6369
                     /locus_tag="PLM74_01665"
     CDS             5206..6369
                     /locus_tag="PLM74_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HON41823.1"
                     /translation="MGTNNDVLGKRIIICVLWLALVIPGGYQLADHRSYMEPVALGPG
                     VTEVKKLSDWAPYIEGTVNDANVYVLDSGVPGGTAVIIGGTHPEEPAGVLSAVTIVEN
                     CVPTSGRLIVLAHANRSASTVTRPGDAYPQFFSIATDFGVRKFRMGDRWTNPLDSWPD
                     PEVYIHYPSKQLLAYMDVRNLNRTWPGRADGLLTERTCAAITDLIRRENADVFIDLHE
                     AELEYSVISTIVAHQNAADIAAMVSMDLSASQFKMGMEYSPQTLHGLSHREVGDLTNA
                     LAFEIEVPEPFLDRVRGVTDVDLLLSGKDEFVMRAGQHGLLYEEIDENGWSVDERVGR
                     TNSVIDAIIQMFSDFNPDRAISYDGLPGRDEIVANGVGRYLLDPSSAKEVVYD"
     gene            6427..8046
                     /locus_tag="PLM74_01670"
     CDS             6427..8046
                     /locus_tag="PLM74_01670"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HON41824.1"
                     /translation="MKQNRRTAFVACVVAALVLLGSSSIFACTDVVVGKDASVDGSTM
                     TSHTVDEGGGPGPLYDCRVRVIPGKIFPAGSTVPIYRQLCEVGPNTPLEKVGEIPQVE
                     KTYTYFHAAYPFMNEHGLTIGETTISQRPELMPSSQAILTVEQLEAIALQRCKTAREA
                     IELIGSLVEKYGFQSSCSDMGECITIVDGKEAWVFEMFGVGPFWLADSGEPGAIWAAR
                     RVPDDEVCVVPNVSRIGVIDETSPNFIVCKHCKDIAIENGWYDPNSGKPFSITEAYTA
                     PTGYWSAESIWTRNRLWYIYSLLCPSQQWDHDAPLSSYPFSVKAEKKVSVQDIIAFQR
                     SAFEGTDRSLSDLPQWFVPDGNGGKVKSPLATPFINSDLAALLGIQNERPIARYKSAH
                     GWVAQVRDFAADPVRTCLWYYSDNPTNSLYVPMYNGVTELPASWGTCDKEKYSRDSAW
                     WAFATVDLLASVRYQEASKDIVAARGPVEADFFATQPAVEKMVADLAAKDPAAARSFI
                     TSYTATCLNRAENLYWDLADLLFTKYNNNRF"
     gene            8376..8924
                     /locus_tag="PLM74_01675"
     CDS             8376..8924
                     /locus_tag="PLM74_01675"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HON41825.1"
                     /translation="MRVTAFCGSPRVNGNTRALVDAVVRGAGSGGAECDVVDICRLSI
                     APCRACSSCRRTGECIQKDDMQSLYPKLLESDVLVFGTPVYFWGASAQMKAFIDRWYA
                     IAGGDAAERLRGKKAVVVTAYADTDPDTPEHVVGMFRRALDYLGIELVGVLGVSASDP
                     GDAASNAQAMQEAFSLGKALCN"
     gene            complement(8921..9394)
                     /gene="bcp"
                     /locus_tag="PLM74_01680"
     CDS             complement(8921..9394)
                     /gene="bcp"
                     /locus_tag="PLM74_01680"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011173357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HON41826.1"
                     /translation="MGKLEIGQLAPDFALPNQDGKTVRLTDFSGQRLVVYFYPKDNTS
                     GCTREAEGFRDLHDAIAAVGARVVGVSRDSVSSHAKFHSKLQLPFDLLSDPEHSALEA
                     YGAWGTKTMYGKTSEGTIRSTVIVDEEGRAAAVFAKVKPDGHAQEVLAALAALSD"
     gene            9639..9824
                     /locus_tag="PLM74_01685"
     CDS             9639..9824
                     /locus_tag="PLM74_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41827.1"
                     /translation="MSRRVQDPVADLTAGRQAEAGSLSPRALAGLFATAGFGGRPGRG
                     GASRTCALWDRIFARPV"
     gene            10070..11248
                     /locus_tag="PLM74_01690"
     CDS             10070..11248
                     /locus_tag="PLM74_01690"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HON41828.1"
                     /translation="MRRPRRSLSSTSLIRSFNVVSVLQTLYREGACSKARIAEITRMS
                     PATVTRIVSELVRQGVVAEHDIAESTGGRKPVIFRLSYDKLYIVGIQLIRDRVTLAVC
                     DLRGKVLDKRTFNPYSLQPSDVVSEIAGEFELLVTLNEIDRQHILGIGLAISGIVNSE
                     SGVLLRSVNLGWQDVRIRELLEQALGFRVVVENDANAAALAEFWFGCAGDVSSFMYLK
                     TDTGVGAGIVCERALMTGARGMAGEIGHVPLVKDGCECVCGQRGCLETYLYGQRLLRR
                     YESHTGMHLDGVRDFFAYAAAGDPVALSMVGEAAGVIGTAVAMAATLLDLDLVVIGGL
                     WGELGDSLIQPVQAKAVEILDRSGLDRAFAVRSSALGEDSDVLGAAGTVINRWFTPPI
                     "
     gene            11353..13053
                     /gene="nagZ"
                     /locus_tag="PLM74_01695"
     CDS             11353..13053
                     /gene="nagZ"
                     /locus_tag="PLM74_01695"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HON41829.1"
                     /translation="MTALSELDVLIGQVLITGFEGPELDADFEHHYLKCPSGGFILFG
                     RNAASATQVKALTEGLRDVARTHNPACPAPLIAVDQEGGSLSPLRGIVSSLPGNMGLA
                     ATGDPEAARYAGYMTGCDLRSLGINVNFAPVLDLAREPLNPAVGTRSFGDDRDRVATF
                     GREYARGLAEAEVLFTVKHFPGHGSSPEDSHVSMPECPMSVEDLARQDLFPFVQAVKW
                     PGTAVMVTHVKFTAIDETRPASLSRDVITGLLRGKMGFDGVVLTDCLEMGGIRPIGSV
                     PEAAVMAIEAGCDMVLVSHTRALQEAAFAAIRDAVRCGRLSVSRLEQSAERIRRWRQG
                     IGATASGGSSPEFGTRWNQELLGERVVTYVPGEAAPGSTPKQSCERSCNQSSGQSSAP
                     TPVCGRGPMVLVTPEMSRITLAEDATDVSLLKHELERYGIHCAMVSCSMDPDAGEIHH
                     VVAEITAASGDRGLCKSRLGEPRARVALVVRNGGTASAQAAQAALAAAIEAKADELGW
                     VFALVCVRDPRETRAVQEALVRRAPAFFTYSTESIILSALSRVLAGEASPRGVMPVRM
                     "
     gene            13112..14434
                     /locus_tag="PLM74_01700"
     CDS             13112..14434
                     /locus_tag="PLM74_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HON41830.1"
                     /translation="MSFGKSGLRIAAAFTLLVSLFLVGASAAAKDPIVLRVLYYFDAT
                     SPGAQRELTEVWEAFDKANPDIKVVREDLFNEPFHQKTEIYAATGQLPDVIYMWPGGR
                     STTLHTKKLVKDLAPLLGAARSEFSEAALAPQAGGYLAELPIGITSTHVLYVNEKMLK
                     DLGLTVPKTYEELKAMAPKLKAAGKDVILMGAQDDWVMQSCLYSAIVGRMLGDKYVDD
                     LIAGKAKFTDAPFVNTLKFYKQLYTDGVLSVKSLQTPYNEVNGLFASGKAPFMIDGDW
                     KVGNFLTDATTGEALISPDKQKDIGMTVFPAIPGEINHQTTSTVVGVGFGMNANIPAG
                     SEKERAAWKLIMWLNSPEVQKERLETGGAFPSRKGVTSDKLEPLAQTRAGFYGAYGGT
                     YVLDNVLEASVYLPINVGLQEIGLGLATPEQVAARVQKAYDAWKAAQK"
     gene            14592..>14775
                     /locus_tag="PLM74_01705"
     CDS             14592..>14775
                     /locus_tag="PLM74_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41831.1"
                     /translation="MKPDAAVVGPGRGGTAAISPGSVKRTKISGARREERWAYVLLVM
                     PAVLVYWAVMAFPAFFS"
BASE COUNT         2979 a         4093 c         4627 g         3076 t
ORIGIN      
        1 ccgctattgt tggtcgcgcg atgggcataa gggtaattgg tcttgtaggc cgtaatgggg
       61 gcgccttggc tcccatttgc gatacagcga taagggttcc atcagtctct actgctgaga
      121 tacagcagat gcatgtgtcc gtgtatcatc atgtctgccg agaggtagag cgaaggctgg
      181 cagcgcaggc ttgacgcggt catgtgtccc gggggttccg ccgtgtgcct gcaagtcttg
      241 ccagaactgc gggcgacacc gcgtttagca tggagttagc ggacctgtaa caatactgcc
      301 cggcttgtac gccggcctgt tcagatggtg cccggttttg aatcggggca ggcaaaaccc
      361 gggagtttgt ggcgtttaat ggggagcttt catcctccga ttgcctcccc aggcatatgc
      421 gagggataga ggcgcgtgac caccttatgg aaaacgcttc tgcctgcaga ttcaaacgcg
      481 acgcgatgac agaaaaaggc acagcccctg ccggcgcgaa ttcagacaat tatcgcccgt
      541 caaactaggt aggatctgaa caggttgctc aaaaaagctg ctcagaaatg gacaggatgc
      601 cccagaaccc cgtcagggcc aaccacagaa gaacgtcacg cccaggaata accatgggca
      661 taggtagtac acagagctag atgtgcactt cgcaacgaac gtgcagcgta cggcgcatat
      721 acactgcttg ctttccagcg tgacgtgttt actcccgcgt cggtttccca tagacagggt
      781 ctcccataga cggggttgac cttgcgccct agacctgcgc atagtaccat gacaagtagc
      841 accacctcag aatcgtacat gtgagttgat cgcagagatg cagcgttccg tagctacggc
      901 caacccgaaa tacggcatcc ttatggccct gactgccgcg attgtttggg gcactcttgg
      961 cgttttcgcg aaagccatct acaagtacca tctagatccg ctcgttctgg tgactatgcg
     1021 tgccggaatc gcattcgtgg ctctgttggc catcatgctg tttcgctctc ccgggttgct
     1081 taaggtggag cggcgcgaca taatcgattt cgcttacgtt ggagtagtcg ggattgccct
     1141 ggaatactgg gtgtacttcg agacgctgaa gcgcacgtcg tcgtcggtcg cttctatctt
     1201 gctgtacact gcgcccgttt tcgtgacggc gggcgccgct gcgttcttcg gggagaagat
     1261 cacccggcgc gggctggccg cgctgggcgt tgttcttatt gggggtttct tggtggctgg
     1321 cgggctagag gcgcgggttc tcacgcagga tcgcgtggga gtgctgttcg gcatagcgtc
     1381 ggcactgacg tacgcaggct acaccctgct cagcaagcgc accctgccca agtacggttc
     1441 gtggaccaca gtcctttatg cgttcggttt cggaacgatc cttctggcgc tgatgtcagg
     1501 gcccagactt gcccaactgg caacggtccc gttcgaaggc tggatgctca tgctgggctt
     1561 ggccctcggg ccgacgcttc tgtcatacgg cctctacgtc aaatcgcttg agtacataga
     1621 ggcgagccgc gcaacagtgg tctgcatgat ggagccggtg acagcgagca tccttggctt
     1681 cctactgctc ggcgagagac tggccggatg gcagctcgtc ggcgctttgt tggttctggg
     1741 cggggtcggt gttctgaatc ttcgggcgag tccgccgctc cccggcgatg tggcctcggt
     1801 ggagggctct cattgaacac gtgcactgta cagccggatc agagagtatt cgcagtatat
     1861 gcagggctga ccgccggcgg gtacgacatc gcacagatcg atcccggtga tgagatagcc
     1921 gcagtagcac aagcaatcct cgaagcgcct tggccggccc atgtacttga ctacttccgg
     1981 aacgctcggg cggccacttg cgaggccaac tcatactggc cccgggcgta catgctggtt
     2041 gccgctgtga tggaggtatc ttcggcgtgc agccgaggtt atcccgatcc cgtacgtgtc
     2101 agaggcatca tggaggccat gcctgtggat cccgcacacc gggatccggc caccatcgaa
     2161 tggctgatgg gcttgcctga agttagtcat catccccaat ccgctgcagg cacggcagat
     2221 agcggatgga gtcgtggctc aagacgcaac ccacctgatc ctcgctcgac cggactccgg
     2281 gtcgatagtg cacgaggtgc ttcacagcct gttcgcgcag gcgctggagt gtgtcaggcc
     2341 ggcggtagat gaggggaacg ccctgtatgc gccggtgcgc gactcgatga tccgcatggg
     2401 ttacgcctgg gatgacagcc ctgcctcgtg gcgacgcgtg tgggaagaga gcctagtgag
     2461 ggcgaccgcg atatgggctg agtgcggcga ctgcccggcc gaagtcgcaa gagcggcggc
     2521 aggccacgcg agcatggggt tcatctatgt tcccaagctg ctccagtgtt ttgcaggctc
     2581 ctgtagcggg ctcaacgatg tcgccgggtt catcctggaa tgcctgaagg tgtgtgcagg
     2641 aacggttctt cacccgtgag tatgcaggcg gcaacgccgc tgccgctatc atggttgcca
     2701 agtgactacc ggttcacctt ggcctagccg tcaaagcgct atggtgatta acagcagcgc
     2761 cagcaaccgg gtctgttgct accaacacca ggtatgtcga atcagccgct cggcaggcag
     2821 gttgacagca acctggtgcc ttacatgacg tcaagcagaa ttatcatcca gtgtacagat
     2881 gcggcaggag tccgaactcg cgcatcgaaa tatgcggtct gtgattttca tcacgattac
     2941 tcaggggggt gtcatgccaa tgatttcgag caacagaaga atccaaatca cactagggct
     3001 cattgcagtc ttgatggctg gcttggcaag cgcagcgccg actgcgcccc agggaattga
     3061 tatgccatcg aaggcgccga cgctgaaggc ccctgtagcc gtgactacca taggtcaagc
     3121 tccgagcgcc atgatggtca ggttgctttt gcagaaagcc aagatccagg ttgtccagaa
     3181 ggacttgttg acgcctcaag acatcgctga ggctgcgaaa aacccgaacg ctgcgttcaa
     3241 gacactgatc atgacgatgg gaacgagcct caagggaatg ggcggagccg gcgtcaatgt
     3301 agattcagag gtgactaggt gcaatgctct ggttgccgaa gccaagaagc acggaatagt
     3361 tgttgtgggc gtccagattg agggtgctgc aaggcggagc gacgaatccg acgaaaagtc
     3421 catccgggct gttgcccccc aatctgacgt gctgatcatc agacgcgagg tcgacaacga
     3481 caactacttc accaacatgg ccaagaagaa tggcgttccg ataatcaggg ccaaggaagc
     3541 cgccgacttc ggttacgtgt tcggaacgtt gtttggttct ccggctaagt aagcttccag
     3601 aggtggtgtg agtcgtgttc ctccacgcag caatagttct agcggtgatg gcagcagcat
     3661 tcgcagtaag catcgctcta cacgccggca gcgaaatagc catgtttgcg gcggctctgg
     3721 caggcgcttt ggcacacgga gccggagtgc ccgcacgcca catcgtagag ggcgcgttca
     3781 cttacttcga cgttatcctc atcttcatca ccgccacatt cttcatgaac ctgctcaaag
     3841 aggcgggagg cgcagcccac atagtgcggg gcatcatctc caagttcagc agaaaccgtt
     3901 tcatgctgct tcttctgctg actttgatca tgctagttcc cggggctctg actggatcgg
     3961 gcacggtgac ggtattagta gtcggggcgt tcgtcgggtc tgtgttgagc tatatgggga
     4021 tatcaaaaga ccgcgcagcg gccatcgtgt tcctatgcgc atcgatgagc gccgctgcgc
     4081 cgcccatcaa cctgtgggct atgatggcgg ccgcaggcgc caacatgcca tacgtagggt
     4141 tcaccattcc gcttgcggtc atctccatcg ccggcgctct gttcagcatg ttctacctgg
     4201 gctggcgtgg gcgcgatgtg gacgcggcca gggcccttcg agagatccct gagcctccac
     4261 acggcatgaa ctggaccaag gtgcttgttc ctttcctgat cttcttcggg gtagtagtgg
     4321 cgggtagggt atggcccctt acagcgcctg tcgtaggtct gccgctggcg tttgcgcttg
     4381 ccggcgcggc tgtggttttg ctgagcccag tcaaactcga cttgtttggc atcctctcaa
     4441 agaccataga cgacctgctt ccccttctca gcacggtgac gatagtaggg atgcttgtgc
     4501 agatcatggc gctatcgggc gccaggggcc tgatatcgtt gtcggtagtg acgcttccca
     4561 tcgccgtgct gtttgcgacc ctcttcctcg tgctgccgat ctcggaaggg atcttccagt
     4621 ttgcagccgc tccgctccta ggcgttccgc tggtgttcct gttcaacatg aagggctata
     4681 acccggtcat ctcgctttcg ggtatggctg caatgtggcc tctgggcgac tgtctgccgc
     4741 ccaccgcacc ggtgggacgc gccgctgtga tggcgaccgg atacgacggc gactactgga
     4801 cgttcctgcg caggtgcata gtacccgcag tcttcatcat ggccataggc actatgtatc
     4861 tggtgtactc ccacaagctc gggttcttgg ctcgcggcta agcgcggctg agcgcagccg
     4921 gacgcgtctg agccgggagc cgggctaggg gtaggactga gacaggaacg gaaacggagc
     4981 cctgcggagg taggacgata cggaggtagg atgaatggaa gcagtgcaga tggtcatagg
     5041 cgttctctac tacttcatga ccgcagtgat agctttggta ataggggcga gcttcttccg
     5101 ccagcgcgat ctcagaaaga tgatcctgta tgcagcggtg ttgatcccct ttgtcctgcg
     5161 ggcgctccgg gtcaagtgac ggtggaagga gacgtgacgc gccaaatggg tactaacaac
     5221 gatgtactcg ggaagcggat catcatttgc gttctgtggc ttgcactcgt gataccaggc
     5281 ggataccagc ttgcggacca cagaagctac atggagccag tggcgctcgg ccccggcgtg
     5341 acagaagtga agaagctgtc ggactgggcg ccatatatcg agggaacggt caacgacgcg
     5401 aacgtgtacg tgctcgatag cggagttccg ggcggcactg ccgtcatcat cggaggaacc
     5461 cacccggagg agccggcagg tgtgctttca gcagtcacca tagttgagaa ctgcgtgccg
     5521 acttcaggcc ggctgattgt gctggcccac gccaacagga gcgcatccac agtcaccagg
     5581 cctggcgacg cgtatcctca gttcttcagc atagccactg actttggggt gcgcaagttc
     5641 agaatgggcg acagatggac taacccactg gactcgtggc ccgaccctga agtctacatt
     5701 cattatccga gcaagcaact cctcgcctac atggatgtaa ggaatctcaa cagaacatgg
     5761 cccggtcgcg ccgacgggct gctcactgag cgcacgtgcg cggccatcac cgatctgatc
     5821 cgcagagaga atgccgacgt attcatcgac ctgcatgagg ccgaactgga atattcggtc
     5881 atcagcacga tagtcgcgca ccaaaacgcc gccgatatcg cggccatggt atctatggat
     5941 ctctcagcga gccagttcaa gatgggtatg gagtactccc cccagaccct tcatgggctt
     6001 tcgcataggg aagtcggcga cttaacgaac gcgttggcct ttgagatcga agttcctgaa
     6061 ccgttcctcg atagggttag gggagtaaca gacgtggacc tccttctttc cgggaaggac
     6121 gaattcgtca tgagggctgg ccagcacgga ctgctctacg aagagataga cgagaatggc
     6181 tggtctgtcg atgaacgcgt aggacggacc aacagcgtaa tcgacgccat catccagatg
     6241 ttttccgact tcaatcccga ccgggcaata tcctatgacg gactgccagg gcgcgacgag
     6301 atcgtggcca atggtgtcgg ccgatatctg ctggatccgt cgtccgcgaa agaagtggta
     6361 tatgactgac aaggtgtgac cgggaggcag cgttacccac aagaaatcgc aaaggagcat
     6421 caagacatga agcagaaccg cagaactgca tttgtagcct gtgttgtcgc tgcgctcgtt
     6481 ctactcggtt caagcagcat ttttgcctgc acagacgtag tagtaggcaa agacgcttca
     6541 gttgacggat ccacgatgac ttcgcatacc gtcgacgagg gcggcggccc cggtcctcta
     6601 tatgattgca gggtccgggt gatacctggc aagatcttcc ccgccggaag caccgttccg
     6661 atctaccgtc agctatgcga ggtagggccg aacactcccc tggagaaggt cggagagata
     6721 cctcaggtag agaagaccta cacctacttc catgcagcgt atcccttcat gaacgagcac
     6781 ggcctgacga tcggcgagac gaccatatcc cagcgccctg agctgatgcc gagctcgcag
     6841 gcgatcctca cagtcgagca acttgaggcc atagcgctgc agcgctgcaa gactgctaga
     6901 gaggccatag agcttatcgg atcgcttgtc gagaaatacg ggttccagtc gtcctgcagc
     6961 gacatgggcg agtgcatcac tattgtggac ggcaaggaag cctgggtgtt tgagatgttc
     7021 ggagtcggcc cgttctggct cgcggacagc ggcgagcccg gcgcgatctg ggcagcccgc
     7081 cgcgtgcccg atgacgaggt gtgcgtggta cccaacgtca gccgcatcgg cgtgatcgat
     7141 gagaccagcc cgaacttcat cgtgtgcaaa cactgcaagg acatcgccat cgagaacggc
     7201 tggtacgatc ccaattccgg aaaacctttc agcatcaccg aggcgtacac cgcccccacc
     7261 ggctactggt cagcggagtc catatggaca cgtaaccgac tgtggtacat ctatagcctg
     7321 ctctgcccgt ctcagcagtg ggatcacgat gctcctctgt cgtcctatcc gttctctgtg
     7381 aaggcagaga agaaagtgtc ggtccaggac atcatcgcct tccagagaag tgcctttgag
     7441 ggcacggatc gctcgctgtc cgaccttccg cagtggttcg ttccagacgg gaacggcggc
     7501 aaggtgaaga gcccgcttgc cactccgttc atcaactccg acctggcggc gctgctggga
     7561 atccagaatg agcgcccaat agctaggtac aagagcgccc atgggtgggt ggctcaggtg
     7621 cgcgatttcg cggcggatcc agtccgcaca tgcctctggt actacagcga caacccgacc
     7681 aactcgctct acgtcccgat gtataatggc gtgactgagc taccggcatc gtggggcacc
     7741 tgcgataagg agaagtactc aagggattcc gcgtggtggg catttgccac tgtggacctt
     7801 ctggctagcg tcagatacca ggaggcatcc aaagacatcg ttgctgcgcg cgggcctgtc
     7861 gaggccgatt tctttgctac gcagccggca gtggaaaaga tggtcgctga tcttgcagcc
     7921 aaggatcccg cggctgcacg cagtttcatc accagctaca ctgcgacgtg cttgaaccgt
     7981 gccgaaaacc tgtactggga cttagcagac ctactcttca cgaagtacaa caacaacagg
     8041 ttctagcgct tcgcacggct gcctcccgtg cacttgcgtt acgcaacagg aaagggcttc
     8101 tcagaaaagt acagctagtc tgggctttgc gaaggaagcc cttcctctat atctcatacc
     8161 gggatctccg cttcttcgtg ccgcgtggcg ttcacaggct caggccattc ggcaaagcct
     8221 gtttgcgcac ggtattgctc atcgttggcc tgttaaggcc gctttggcgg tcagctacga
     8281 tacccggtgc gacaatagtc ggatagaagt ggtatgctgt cctatagtgg ggcatctgct
     8341 atcagacgag agcgtcatga ttgggagttg atcatatgcg agttacagcc ttttgcggga
     8401 gtccgagggt gaatgggaac acgcgggcgc ttgtggacgc ggtcgtccgg ggagccggca
     8461 gcggaggagc tgaatgcgat gttgtggaca tctgtcgcct cagcatagct ccgtgtcggg
     8521 catgttcatc gtgcagacgc accggagagt gcatccagaa ggacgatatg cagtctctgt
     8581 acccaaagct tcttgagagc gatgtgctcg tgttcgggac tcctgtgtac ttctggggcg
     8641 cgtcggcgca gatgaaggcc tttatcgata ggtggtatgc tatcgccggg ggagacgcgg
     8701 ccgagaggct tcggggcaag aaagctgtgg ttgtcaccgc atatgccgat actgaccccg
     8761 acacgcccga gcatgtcgtg ggcatgttcc ggcgcgcctt ggactacctc ggcattgagc
     8821 tggtgggggt actgggcgtg tcggcgtccg accctggcga cgcggcatcg aacgcccagg
     8881 ccatgcagga ggccttcagc ctggggaagg cattgtgcaa ctagtctgac agagccgcca
     8941 gcgcggctag cacctcttga gcgtggccgt cgggtttcac tttggcaaag acggcggctg
     9001 cgcgaccttc ctcatctact atcacagtgg accgtattgt cccttcggat gtcttgccgt
     9061 acatcgtttt ggtgccccag gccccgtatg cttcgagagc gctgtgctcg gggtcgctca
     9121 ggagatcgaa aggcaattgt agcttgctgt ggaacttggc gtgtgatgac actgagtccc
     9181 tgctgactcc caccacacga gcgccgaccg cagcgatcgc atcatgcagg tcacggaaac
     9241 cttcggcctc acgggtacag ccggaggtgt tgtctttcgg atagaagtac accacaagtc
     9301 tctgccccga aaagtctgtg agacgaactg tcttcccatc ctggttcggg agcgcgaagt
     9361 ccggagccaa ctgtccaatc tcgagcttgc ccatgtgcgc attgccccct ttatatgcta
     9421 gcggtgttct aacggtagtg tagcagagca tgggcctgtt gggcaatcga gccgtgtcgc
     9481 cagactgcga aaataccccc ttttggggtc aatccgccta agaaagcgtc ctccctgtag
     9541 ccgggcgccc cggtcgcgga ggggcgtcgc ggacctgtgc cctgtgggac agggttttcg
     9601 cacgtcctgt gtaaactgac acaggatggc cccaaaaggt gtcccgtagg gtacaggatc
     9661 cggtcgcgga cctcaccgcc ggccgccagg ccgaggcggg cagtctcagc ccacgcgctc
     9721 tagctgggct gttcgcaact gcgggcttcg gcgggcgccc cggtcgcgga ggggcgtcgc
     9781 ggacctgtgc cctgtgggac aggattttcg cacgtcctgt gtaaactgac acaggatggc
     9841 cccaaaaggt gtcccatagg gtacaggatc cggtcgcgga cctcaccacc ggacgcgagg
     9901 ccgagggcat gagatgggcc gagggcatga gatgggccga gggcgtgaga tgggtcgaag
     9961 cgtgagatag gcaatggcat tgacaatgcg aacgctatgg gctatcatgg cattaagata
    10021 tttgcatact gaacgaaagt tgaaacagag gctgaaagag ggtcacttta tgcgaaggcc
    10081 gaggcggtcg ctatccagca ctagcctgat aaggtcgttt aacgtggtct cggttctgca
    10141 gacgctgtat cgggaaggcg cttgttcgaa ggcaaggatc gcagagatca cgcggatgag
    10201 cccggctacg gttactcgga tagtctctga gctggttcgt caaggcgtgg ttgcagagca
    10261 cgatatcgcc gaatctactg gtggccgcaa gcccgtgatc ttcaggctca gctacgacaa
    10321 gctctacatt gtcgggattc agttgattcg cgaccgagta acgttggctg tgtgtgatct
    10381 cagaggaaag gtgctcgaca aacgcacgtt caacccttac tcgctgcaac cgtccgatgt
    10441 ggtatcggaa attgcaggag agttcgaact tctcgtcaca ctgaatgaaa tagaccgaca
    10501 acatatcctc ggaatcggcc ttgccatatc aggcatcgtc aactcggagt cgggcgttct
    10561 actgcggtcg gtcaatctgg ggtggcaaga tgtacggatc cgcgaattgc tggagcaggc
    10621 actgggtttt cgggtagtgg ttgagaatga cgccaatgct gccgcccttg ccgagttttg
    10681 gtttgggtgc gccggcgatg tgtcgagctt catgtacctc aagaccgaca ccggcgttgg
    10741 agccggaatc gtgtgtgaac gggcgctgat gaccggggcg cggggaatgg cgggcgagat
    10801 tggccatgtc cccctagtga aagacgggtg cgagtgcgta tgcggccaac gtggctgcct
    10861 cgagacgtat ctgtatggtc agcgcctgct gagacggtac gagtcccaca caggaatgca
    10921 tctcgacggt gttcgcgatt tcttcgccta tgccgcagcc ggcgacccgg tggctctgtc
    10981 aatggtgggc gaagcagccg gggtgatcgg taccgcagta gcgatggctg ctacactgct
    11041 tgacctcgac cttgtagtaa taggtggtct gtggggggaa ctgggcgact cgttgatcca
    11101 gccggtgcag gcgaaggccg tcgagatact cgaccgcagt gggctcgacc gagctttcgc
    11161 agtgcgaagc tcagcgctgg gcgaggactc ggatgttctg ggcgctgcgg gcaccgtgat
    11221 caacaggtgg ttcactccgc caatttagcg acgtggcagg ttccgacatc gaggtgtgtt
    11281 ctagcgccag ggtgacaacc tagtaggccc acttctttgc gatatgatag aaagatttcg
    11341 agaggtatcg acatgactgc gttatcggaa ctggatgtcc tcatcggcca ggttctcatc
    11401 accggatttg aagggccgga gctagatgcc gacttcgagc accactatct caagtgccct
    11461 tctggcggat tcatactgtt tggcaggaat gcggcgagcg cgacccaagt gaaggcgttg
    11521 accgaagggc tccgcgatgt agctcgaacg cacaatccgg cctgcccagc gccgctcatc
    11581 gccgtggatc aggagggcgg cagcctgtcg ccgcttcgcg gtattgtgtc gtctctgccg
    11641 ggcaacatgg gtttggcagc gacgggggat cccgaggccg cgagatacgc cggatacatg
    11701 acgggatgcg atcttcgatc cctcggcatc aacgtgaact tcgcgcccgt tctcgatctc
    11761 gcacgcgaac cgttgaatcc tgctgtgggc actcggtctt tcggcgacga ccgtgatcgt
    11821 gtcgcgacat tcggcaggga gtacgcgcgc ggattagcag aggcggaagt cctgttcacg
    11881 gtgaagcatt tccctgggca tggtagttcg ccggaagact cgcatgtctc gatgcctgag
    11941 tgcccgatgt cggtggagga tctcgcgagg caggacctgt tcccgtttgt ccaggctgtc
    12001 aagtggccgg gcacagccgt catggtcacg cacgtcaagt tcactgccat cgatgaaacg
    12061 cgtccggcct cgctttctcg cgatgtgata accggattgc ttagagggaa gatgggtttc
    12121 gatggggtag tactcaccga ctgcctcgaa atgggcggaa tacgtcctat cgggtcagtg
    12181 cctgaggccg cggtaatggc catcgaagct ggctgcgaca tggtcttggt gagccacact
    12241 cgcgccctgc aggaggctgc attcgccgcg atccgtgacg cagtccgctg cggcaggctc
    12301 agcgtttcga gactcgagca gtctgccgag cgaattcgcc ggtggagaca gggcataggg
    12361 gcgactgctt cgggcggctc gtcccctgag ttcggcacac gctggaacca ggagcttctc
    12421 ggcgaacgag tagtcaccta tgtgcctgga gaggcagcgc cgggatccac acctaagcag
    12481 tcatgtgaac ggtcatgtaa ccagtcatca gggcagtcgt ctgcgccgac gccggtttgt
    12541 gggcgcgggc ccatggtgct cgtgacgccg gagatgagcc ggataaccct cgcggaagat
    12601 gcaactgacg tatctctcct caagcatgaa ctcgagcggt acggtatcca ctgtgccatg
    12661 gtcagctgct ccatggatcc ggatgccggc gagatccatc atgtagttgc ggagataact
    12721 gcagctagcg gcgaccgagg cttgtgcaag agcaggctag gtgagcctcg tgcaagggta
    12781 gcgctggtgg tgcgcaacgg cggcacggct tccgcgcagg ctgcgcaggc ggcgctggcg
    12841 gcggcgattg aggcgaaggc cgatgaactg ggctgggtct ttgctctggt gtgtgtgagg
    12901 gatccgcgcg agacccgagc cgtgcaggag gctctcgtac gacgggctcc cgcgttcttc
    12961 acctatagta ccgagtcgat aatactgtcc gcactctcgc gggttctggc tggcgaggct
    13021 agccccaggg gggtgatgcc tgtcaggatg tgatggctct aaagcgatat gacgctgcgt
    13081 ggtccaaaga tcatgtatag gaggtaatca catgtctttc ggcaagtctg gtctcagaat
    13141 tgctgcggca ttcacgctcc tggtctcgct tttcctcgtg ggggcgtccg ccgcggcgaa
    13201 ggacccgata gttctcaggg ttctctacta cttcgatgcc acgagcccgg gagcgcaacg
    13261 ggagcttacg gaagtatggg aggcgttcga caaggctaac cccgacatca aggtcgtgcg
    13321 cgaagacctc ttcaacgagc cgttccatca gaagacggag atctatgccg ccactggcca
    13381 gttgcccgac gtgatttaca tgtggccggg cgggcgctcg accaccctgc ataccaagaa
    13441 gctggtcaag gaccttgctc cgctgcttgg agcagccaga tccgagtttt ctgaggcggc
    13501 tctggcgccg caggccggcg gctacctggc cgagctcccg attggcatca cctcgactca
    13561 cgtgctctac gtgaacgaga aaatgctcaa agacctgggg ctgacagtgc ccaagaccta
    13621 cgaggaactc aaggcgatgg cgcctaagct caaagcggcc ggcaaagacg taatcctcat
    13681 gggcgctcag gacgactggg tgatgcagtc ctgcctgtac agtgccattg tcggcaggat
    13741 gctgggagac aagtacgtcg acgatctgat cgccggcaag gccaagttca ctgatgcgcc
    13801 tttcgtgaac acactcaagt tctacaagca gctctacacc gatggagtgc ttagcgttaa
    13861 gtcacttcag actccctaca acgaggttaa cggcctgttc gcgtcgggta aggccccatt
    13921 catgattgac ggcgactgga aggtgggcaa cttcctgacc gatgctacga ccggcgaggc
    13981 gctcatatcg cccgacaagc agaaggacat cggcatgacc gtgttcccgg cgatccccgg
    14041 cgagatcaac catcagacca cgtcgacagt ggtgggtgtc ggcttcggga tgaatgccaa
    14101 cataccggcc ggctccgaga aggagagggc cgcatggaag ctgatcatgt ggctcaactc
    14161 ccccgaagtc cagaaggagc gtctggaaac cggcggggca ttcccgtcta gaaagggagt
    14221 aacctcggat aagctcgaac cgctggccca gacaagagct gggttctatg gcgcatacgg
    14281 cggaacgtac gtgctagata acgtgctcga ggccagtgtg tatctgccca tcaacgtcgg
    14341 tttgcaggag ataggcctgg gccttgccac tcccgaacag gtagctgcaa gggtccagaa
    14401 ggcgtacgac gcctggaagg ctgcgcagaa gtaggctttc aggaagtagc gagacatgac
    14461 atatcgccca gagggccagg gcgggtgtgg cgaagcgcgc catgtcggcg atcagcgtcg
    14521 caggggcctc gccacatccc tggtcctggg tatgcggagc cctgaagaag agactacgga
    14581 ggtgaggtct cgtgaagccc gacgcggcag tggttggccc tgggagaggc ggcacggctg
    14641 caatcagtcc ggggagcgtc aagcggacaa agataagcgg ggccagacgc gaggaacgct
    14701 gggcctatgt ccttttagtt atgccagccg tgcttgtcta ctgggcggtc atggcgtttc
    14761 cagcattctt ctcgg
//
LOCUS       DAOTAC010000020        14720 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_3440_length_14720_cov_4.292124, whole genome shotgun sequence.
ACCESSION   DAOTAC010000020 DAOTAC010000000
VERSION     DAOTAC010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 14720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14720
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3440_length_14720_cov_4.292124"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="PLM74_01710"
     CDS             complement(<1..559)
                     /locus_tag="PLM74_01710"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HON41832.1"
                     /translation="MVELSDRDEVIVDRIALPRTAHAIWFGRGLLQRIEEAQVRTVLG
                     RGSHLKAPTCLAVVGAESSKAAPVADAIARLGRMFTSAVTFQAPGRADHAAIRSGVNA
                     ARRARADVVLAVGGGTTLDVGKAVSALAEHDDAEDVEGFQTGCHRVNPARALPWIAVP
                     TTSGTGAESTNNAVVELGDEKRSIRG"
     gene            740..1909
                     /locus_tag="PLM74_01715"
     CDS             740..1909
                     /locus_tag="PLM74_01715"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HON41833.1"
                     /translation="MKRRAIAVTAIALLAVAAVAGGVAWRRRQKAAQSTAVPSYRPVP
                     VSRGTLSRSISATGNLSAAGQIDVYPEKSGVVDAVLVDVGREVKTGDVLVRLVQDQID
                     VQQASSSVRQREEELRSAQDMLGRIRDLHGRSAATDKELRTAESSVVQAQDSLQAAKL
                     KLDGLVTHSGDCLIRSPIDGVVTAVKAVVGGSVSPSAAVVTMVDPSNVVLKVTVDEND
                     IGVVQVGQEANITLDAVDGLTLAGTVSAVGKVGETKSGIVVFNVDIGVDNPDPSAMPG
                     MSAEAQITVERVDNAIIVPISALQGRAGRYAVQVRNADGSIEPRSVEVGMKTSTAAEV
                     LSGLSTGDVVVVADGRATSSGSYTRRNSQTGMPGFSMPGMDGGGPGGTPGGMPRM"
     gene            1920..2627
                     /locus_tag="PLM74_01720"
     CDS             1920..2627
                     /locus_tag="PLM74_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON41834.1"
                     /translation="MSFIELKDVHKEYSTGRGSVRISALSGVSLTVEEGEFVSIMGHS
                     GSGKSTLMNILGCLDRPTSGEYILDGHVVGKLSSDQLADIRNRTIGFVFQSFNLLQRL
                     DAVSNVEMPLIYSGVPARERREAAIKALEMVRLGDRMRHRPSELSGGQQQRVAIARAL
                     VGRPRLVLADEPTGNLDSAASREIMEILRQLNNDGITVIVVTHEPEVAAYTPRVLRFR
                     DGVCIEDGAAALGGVRA"
     gene            2624..3796
                     /locus_tag="PLM74_01725"
     CDS             2624..3796
                     /locus_tag="PLM74_01725"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HON41835.1"
                     /translation="MRPADNVRLAMGGLLGSPFRSFLTTLGVIIGVSAVIITVSIGTG
                     AKRQTESQIQRLGSNLITVSSGYRSASSKITNRILPAIEQCGSVRQVTPQINRSGTAS
                     YGSNSLNTSAIGTSSNYLAVRNLKIARGVFLSDDDIESSTWNCVLGADLATELFEEED
                     PLGKRVRFDRVRFTVVGVAASQGDTGFSSVDNVMFIPYTTMQKRLTGNKNISTISVQA
                     VSDKEMDTAYDQVYATLLLETGDERAFRLSSQADVLDFAASMTDTMTLLLSGIAAVSL
                     LVGGIGIMNIMLVSVTERIREIGIRKAIGAKERQILAQFIMEAATLSVSGGLLGIAFG
                     FLGSRIVTRVFSWPTAVDTGSVVLGFSLSLAVGVFFGAYPAYKAAGLDPIEGLRHE"
     gene            3810..5180
                     /locus_tag="PLM74_01730"
     CDS             3810..5180
                     /locus_tag="PLM74_01730"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HON41836.1"
                     /translation="MRTFAIIVCCICAIGVCAAPADAEVSPGNSPSVWSIDEFAEAAL
                     SESRSYLAARESVETAEISERLAIPFYGFGVTLSSVRTGSIGSANSPGPSSESKDGER
                     VSVRAPITDKLSLTANYAIDKSSCTLKLSYTPLSGMTGSDVIRQFPSWLLGSSGPTDA
                     AISAVELAALKLDDARSAALLDARKAYISALKTIKARDIAREEHRLAGLELAIAEKRF
                     GLGLSSELEVEQAKINLLDAELALMKAETDDRWMRKRLSAMTGQDMSSAILEGLPDFD
                     LTAPDLEALVSAAVGRDADLAQAKAELATAVRTLESAKSLLPSVQVDAETTVGDWSPR
                     FTVSANWSISLSRSGEIQKAAIRVEQRQRALDEATTAVTEAVAKNLDYLTIEVASMEK
                     WRRQLEESEESHRKALESYEEGEVLLVDLERARLNLQKARNNYLAHWGAVWEMWYTLV
                     DMSMHE"
     gene            5211..6320
                     /locus_tag="PLM74_01735"
     CDS             5211..6320
                     /locus_tag="PLM74_01735"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HON41837.1"
                     /translation="MKFGNKALIVLLLLPLLTFRGFGNTASAAAAASATTRLEIGLAQ
                     CVAGYVAGSDAVRTAEKKAADSAESYERAVAEKKAALSLWELKICAEAAQLALAEARN
                     NAALLGAQAYIGLAQAVRDAKSKHASLAVAEEKLRVLRLRHQAGLITGDTVLQQENAY
                     LSAKDAVIRADDTLKQAKSDLCRAMAVDLTDEIVLKTDVASLADESVTYDAAGCMKLA
                     RAASSSYFSAVKSEELARQKHQALQDPMIATKAERASAEDAFRDAADKLANAEKSLDD
                     SVANALAQLDSLIRSLRMQVLSSELSDANLGVARIKFGYGEMLQYELDSEVNSVSQAH
                     DKVTKAREDLYVQVLKIESMIGRDVVDMLRQAGLK"
     gene            6367..7044
                     /locus_tag="PLM74_01740"
     CDS             6367..7044
                     /locus_tag="PLM74_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HON41838.1"
                     /translation="MELVKILLADDEPRMRRLVSDFLKREGYALVEAEDGQQALDLFF
                     SEKGIGLVILDVMMPRLDGWVVCREIRKASNVPVIMLTARSEESDQLFGLDIGADEYI
                     TKPFSPMILVARVKALLRRAGGDTRRPRLFGRLEIDSARRLASVDGERVYLSPKEYEL
                     LVYFTDNEGMALSREQILSSVWDYDYFGDTRTVDTHVKRLRAKLGDAAEYIQTVRSVG
                     YRFEALQ"
     gene            7041..8600
                     /locus_tag="PLM74_01745"
     CDS             7041..8600
                     /locus_tag="PLM74_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019224643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HON41839.1"
                     /translation="MIKSVRGKLFAGIVGLIALFVLLSWALNWGLLDGYYRSAKRGSL
                     VNAYLKLRKLYQGEAGALLLELETMERREGFHIVVVDPWQSVKYDTVPDRRGGSAPTP
                     DRLPRPGSDRWLTEFIVRTAQDMDGDYVVLEATPDERLRTEFMSLVGLLANGDYLFLR
                     TPVTAIQESAATANRFFFISGSLMIIVGIVFAFVFAGWFTRPILEMKGIAEKMARLDF
                     SSKYRGSSHDEIGALGASINLMSDQLERSIGELVTANEKLLEDIERERRIDDMRKEFI
                     ANVSHELKTPIAMIQGYAEGLKLNVIEDEENRNFYCDVIADEAARMNRLVSQLLSLAR
                     IESGNAAPDMICFDIVEMARRVLRKSCLVLQERGIDASTEGEERALVSADYDMIEQVM
                     TNYITNAINHAAGDNMLRVRIDSIPGKVRVSVLNSGEPIPLESIDSIWTSFYKTDKAR
                     TREHGGTGLGLSIVKAIQDAHGNGYGASNVDGGVEFWFDVNAAAETGLLETDVPGTDL
                     PETDLDMSPSR"
     gene            8642..8725
                     /locus_tag="PLM74_01750"
     tRNA            8642..8725
                     /locus_tag="PLM74_01750"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8676..8678,aa:Leu,seq:gag)
     gene            8917..9828
                     /locus_tag="PLM74_01755"
     CDS             8917..9828
                     /locus_tag="PLM74_01755"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HON41840.1"
                     /translation="MITVKIGLALGSGGLRGAAHIGVIDELEKAGVRADMVAGTSSGS
                     LIAAMYASGMTPSEMEDAALGITARDILDPIGYTAFFLVAPAFMLLLGAKSYLPKGIL
                     RGRRFERLLTSYFGEMSMSDLKMPCSAVSVDIHTGEKIVFTSESPARAAIFKETVYFN
                     APVVDAVRASCSIPGVFEWKAWGGRYLVDGAVREPVPARILKEQGCDYVIAVDLGYTG
                     QADAQVRELPSIITQSLDILGEEVSDYVLYYYANTIIRPRLYNVALSDTSRIPECIEE
                     GRNAARQALPDLRRSIRRRVMRRLAAI"
     gene            9935..10990
                     /locus_tag="PLM74_01760"
     CDS             9935..10990
                     /locus_tag="PLM74_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HON41841.1"
                     /translation="MDTKQALMHLADARGVTGYESPAWDVVNGLFADLVDEIRRDSLG
                     SAIMLKRGAAKEPPASIMFAAHLDEIGMIVSKIEDEGFLRIWMMGGVDRRILPSMEVT
                     VHGRRDLPGVISAIPPHLQDPSKKDKSWKWDELLVDCGMPADRLRELVRVGDVISFDR
                     RAIGLANDRIAGKSMDDRAGIAVMYEAARELQKLSHECDVYFVATVQEEVGYGGSITS
                     TFGVMPDIGIAIDVGHAAMPGLPEHKVLKMGGGPAISFGPHVHPHIFQKLKETAEDRG
                     IKYQLEPGTNPRGTDAYAMQMTRSGVATGLISVPLRYMHTSVETLALDDVKKSGQLLA
                     YFASAVDSRFRGGLTCF"
     gene            10981..12006
                     /locus_tag="PLM74_01765"
     CDS             10981..12006
                     /locus_tag="PLM74_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HON41842.1"
                     /translation="MLLAKLSEAGGISGQEAEVRALIRSEITPYVDEIKVDPIGNLIA
                     VKRGRKGNRRVMLAAHMDEVGLMVVGIDSDGLLHVEAAGGVDPRVLVSKLVYVGADRR
                     PGVIGSKPIHLLKAEDMDRATDMDSLYVDIGAKNKDDAEKLVKLGDQIIFATRCEPLG
                     EGFLKGKAMDDRVGCYAIIEALKKQHDFDLIGAFTVQEEVGLRGAGVAAYQVHPDLAL
                     VLEGTTAHDVTETPEHGFSTVPGRGPAIVYRDASWVGDRRIVDRLIETAEAAGVPYQI
                     KRTVTGGTDAGRIGPTESGVPVAVMSMPCRYIHSPVSVICADDLNNYVKLAGLFLDSL
                     DERGLPV"
     gene            12003..13043
                     /locus_tag="PLM74_01770"
     CDS             12003..13043
                     /locus_tag="PLM74_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HON41843.1"
                     /translation="MKDLAKKLSESFGPSGSEEQIRSIIRSEVEQYADEIRVDAMGNL
                     ICRVAPKNPVPGSGAGAPPKRIMVDAHMDEIGIVVSFIDDKGFLRFSPVGGLSPHVLL
                     GQKVVFADGTVGVFGTEKLDDIKDLKFDKMFIDIGARDRAEAQAKVRVGDAAAMVSSF
                     ADMGVRVSGKAMDDRIACLVLIEAMRRARPQVHEAYYVFAVQEELGLRGARTAAYGIN
                     PDVGIAVDVTLWGDTPEHAPFNVALGGGAAIKAKDSSLIAHPAVKNLMIDVAEKNGIP
                     YQLEVLERGGTDAGAIALTREGVPSGTISVPCRYVHTPAEMVDMGDVDACVRLLVAMV
                     EELASSTGGGIL"
     gene            13084..13248
                     /gene="scfA"
                     /locus_tag="PLM74_01775"
     CDS             13084..13248
                     /gene="scfA"
                     /locus_tag="PLM74_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HON41844.1"
                     /translation="MVSTDTRKETKHVKTIIAADLKGTAATGGCRECQTSCQSACKTS
                     CTLGNQACRK"
     gene            13179..14642
                     /gene="scfB"
                     /locus_tag="PLM74_01780"
     CDS             13179..14642
                     /gene="scfB"
                     /locus_tag="PLM74_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HON41845.1"
                     /translation="MPDLLPVRVQDLVHPWEPGVQEVARRTLSEQGRPSPTGIRRNWH
                     RFMAAGARLLLDVQSGSLHSLDEAAWDVLEAMEQGKAVGQIDGADDVIADLDVLIAEG
                     TLSHPDDADPMPAALGPGVVKAMCLNVSHDCNMRCGYCFAGTGGFGGERSLMPAEVGC
                     RALDLLFDISGHVRHLDVDFFGGEPLLNMDAVAEITRHGRELEALTGKTLKLTLTTNC
                     YELSPETDRFLNDNDIAAVLSVDGRSGIHDGVRRTADGCPTFERIADNALRFVNTRPT
                     GEYYVRGTYTRRNLDFVQDVLFLADMGFTSISFEPVVAAPSSPYSIREQDLPRVFDSY
                     DRLVTEMAARRAAGHEFRFFHFDLNVAHGPCLAKRLLGCGAGREYVAVTPDGALYPCH
                     QFVGREGFIMGNVWDGITRPEIGEAFAGAHIYAKSACRDCWARYMCSGGCHANAHEMN
                     GTLMTPYSIGCLITRKRLEAALAYAALQGSSQGSSPV"
BASE COUNT         3079 a         4041 c         4723 g         2877 t
ORIGIN      
        1 tcccgcgtat gctccgcttc tcgtcgccca gctcgacgac ggcgttattg gtgctttccg
       61 ctccggtgcc cgaagtcgtg ggaacagcga tccatggaag cgccctggcg gggttgactc
      121 tgtgacagcc agtctgaaac ccttccacgt cttctgcgtc atcgtgctct gcgagcgcgg
      181 atactgcctt gccgacatcg agcgtagtgc ctccgcccac ggccagaacg acatcggccc
      241 gcgcgcgccg ggccgcattc acgccggatc gtatggctgc atggtcagcg cgtccaggcg
      301 cctgaaacgt caccgcgctt gtgaacatgc gcccgagcct ggcaatggca tcggccaccg
      361 gcgcagcctt cgagctttcc gctcctacta cggccaggca cgtcggcgcc ttcagatgac
      421 ttccccgacc cagtaccgtc ctgacctggg cctcttcaat ccgttgcagc agcccgcggc
      481 cgaaccaaat cgcgtgggct gtgcgcggca gcgcgattcg atcgacgatc acctcgtcgc
      541 ggtctgacaa ttcaaccaat agtccggcgc tcctctcatg cgtgatccgc agaacactgt
      601 ctccttgggt tcggcgagcc atcccagtca tcctccttca cgactacatt gccatatgac
      661 gttcacagta cggacacaca gaactggtat cctgtggaca ggttgtgata cacgtcgtga
      721 tgcacggggg tgacgaagca tgaaacgcag agccattgcg gtgacggcca ttgctctgtt
      781 ggcggtggcg gccgtcgccg gaggcgttgc ctggaggcgc cggcagaagg ccgctcagag
      841 tacagcggtt ccttcataca gacccgtgcc ggtctcaagg ggaacgctgt cgaggagcat
      901 atccgccaca ggaaatctga gcgcagctgg ccagattgac gtctatccag aaaagtcggg
      961 cgttgttgac gcagtcctgg tggatgtggg gcgagaggtg aagacgggag acgtgcttgt
     1021 gcgcttggtc caggaccaga tcgatgtgca gcaggctagc tcaagcgtca ggcaacgcga
     1081 ggaagagctg agaagcgcgc aagacatgct cggcaggatt cgcgacctcc atggacgcag
     1141 cgccgctaca gacaaggaac tcaggactgc agagtccagc gtcgtgcaag cgcaggacag
     1201 ccttcaggca gcaaagctca aactagacgg ccttgttaca cactccggcg actgcctgat
     1261 ccgttcgccg atagatggtg tggtcactgc cgtgaaagcc gtcgtaggag ggagtgtgtc
     1321 gccgtcggca gccgtagtta ccatggttga tccgagtaat gtggttctca aggttacagt
     1381 ggatgagaat gacatcggag tcgttcaggt aggccaagaa gccaacatca cgctggacgc
     1441 ggtggacggc ctgactcttg caggaactgt gtccgccgtc ggcaaggtcg gcgagaccaa
     1501 gagcggcatt gttgtgttca atgtcgacat cggcgtcgac aatcccgacc ccagcgcaat
     1561 gccggggatg agcgcagagg cgcagatcac tgtggaacgt gtcgacaacg cgatcatagt
     1621 ccccatcagc gctctgcaag ggcgtgcggg caggtatgcg gtgcaggtac gcaatgccga
     1681 tgggagtatc gagccccgaa gcgttgaggt tgggatgaaa acaagcactg ccgcggaagt
     1741 cctttccggt ctgtccactg gcgacgtagt tgtcgtggcc gacggccggg caacttcttc
     1801 cgggagctat acgaggcgga acagtcagac tgggatgccg ggcttttcga tgccgggtat
     1861 ggacggcgga ggtccgggcg gaactccggg cggcatgccc agaatgtaga ggtatcgcca
     1921 tgagcttcat cgagctgaag gatgtacaca aggagtactc cacagggaga gggtctgtaa
     1981 gaatcagtgc cctgagcggg gtgagcctta cggtagaaga gggcgagttc gtttccataa
     2041 tgggccactc cggctcgggt aagtccacat tgatgaacat cctggggtgc ctggataggc
     2101 ccacgtcggg agagtacatt ctggacgggc acgtagtcgg caagctcagt tcggatcagc
     2161 tggctgacat tcgaaaccgc actatcggct ttgtgtttca gtcgttcaac ctgcttcagc
     2221 ggctggacgc tgtcagcaac gtggagatgc cgttgatcta ctccggcgta ccggctcggg
     2281 agagacggga agccgccatc aaggcgcttg agatggtgag actgggcgac agaatgcgtc
     2341 accgcccgag cgaactgtcg ggaggccagc agcagcgggt tgccatagcc agagctttgg
     2401 tggggaggcc gaggctagtg cttgcagacg agcctacagg caatctggac tccgctgcaa
     2461 gccgggagat catggagatt cttcgtcagc tgaacaacga tggtatcaca gtcatcgtag
     2521 ttactcacga gccggaggta gctgcctaca ctccgcgggt gctcaggttc agagatggcg
     2581 tgtgtatcga agatggtgca gccgcgttgg ggggtgtgcg cgcatgagac ctgcagacaa
     2641 cgtcaggctg gctatgggag gtttgctggg cagccccttt cgttctttcc tgaccacgct
     2701 tggggtgata atcggcgtat cggcggtgat catcacagtg tcaatcggca ctggcgccaa
     2761 gcgccagacc gagtcgcaga ttcagagact cgggtcgaat ctcatcaccg tatccagcgg
     2821 ctatcgcagc gcttcctcga agataaccaa ccgcatcctg cctgcgatcg agcaatgcgg
     2881 ctctgtgcgg caggtgactc cgcagatcaa caggtcgggg acggcgtctt acggatccaa
     2941 cagcctgaac acttctgcga taggcacatc ctccaattac ctggcggtga ggaacctcaa
     3001 gattgcgcgt ggggtgtttc tatccgatga cgacattgag tcttccacct ggaattgcgt
     3061 actcggggcc gatctcgcaa ctgagctgtt cgaagaggag gacccccttg gaaagagagt
     3121 tcggtttgac cgggttaggt tcacagtagt gggcgtagct gcatctcagg gcgacactgg
     3181 tttttccagc gtggataatg taatgttcat accctacacc accatgcaga agcggctcac
     3241 cggcaacaag aacatcagca ccatcagtgt gcaggcggtg agcgacaagg aaatggatac
     3301 cgcctacgac caggtgtatg ccacgctcct tttggagaca ggcgatgaac gggccttcag
     3361 gctgagcagc caggcggatg tacttgactt cgcggccagc atgactgaca ctatgacgct
     3421 gcttctctcg ggcatagcag ctgtgtcact cctcgtgggc gggataggga tcatgaacat
     3481 catgctggta tcagtgactg agcgcatcag ggagataggg attcgcaaag ccataggcgc
     3541 caaggagcgg cagatactag cgcagttcat aatggaagcc gcaaccttga gcgtgtctgg
     3601 cgggctgctg ggcatagcct tcggcttcct cgggtcccgc atcgtcacac gagtattcag
     3661 ctggcctacg gccgtcgaca ccgggtcggt cgtgctcgga ttctcgctct cgcttgcagt
     3721 aggagtgttt ttcggtgcat acccggccta caaggcggcg gggctcgatc ccattgaggg
     3781 gcttcgacac gaatgagggg ggatagtcgg tgaggacgtt cgcgatcatc gtgtgttgta
     3841 tctgcgcaat tggcgtgtgt gctgcgcctg cggatgccga ggtatcgcca ggcaacagcc
     3901 caagcgtgtg gagtattgac gagttcgccg aagccgcgct gagtgagtcg cggagctacc
     3961 ttgcggcgcg cgagtctgtc gaaacggctg agatcagtga gagacttgcc attccattct
     4021 atggatttgg tgttacgctg tcgagcgtcc ggactggctc gatcggctcg gccaactccc
     4081 ccggcccgtc ttcagaatcc aaggacggcg agcgggtgtc agtcagggct ccgatcaccg
     4141 acaagctatc cctgactgcc aattacgcaa tcgacaaatc ctcatgtacg ctcaaactgt
     4201 cgtacacccc gctttccggt atgacaggtt ctgacgttat ccgccagttc ccctcatggc
     4261 ttctgggatc ttcgggaccg accgatgccg caatatccgc agtggaactg gccgcgctca
     4321 aactggacga cgcgaggtcc gcggccctgc tcgatgccag gaaggcctac atcagcgcgc
     4381 tcaagacgat caaggctcgg gacatcgcgc gagaggagca cagactcgcc gggctcgagc
     4441 tggcaatagc ggagaagcgc ttcgggttgg ggctatccag tgagctggaa gtcgaacagg
     4501 ccaagatcaa cctgctcgac gccgagcttg ccctgatgaa ggcggagacc gatgatcggt
     4561 ggatgagaaa gcggctttcg gcaatgacgg gccaggacat gtcaagtgca atcctggagg
     4621 gcttgccgga cttcgatctg acggcgcccg atcttgaggc actggtttcg gccgccgtgg
     4681 ggcgcgacgc tgacctggcg caggcgaagg cagagctcgc cactgccgta cggacgcttg
     4741 aatcggccaa gagcttactc ccgtctgttc aggtggacgc tgagacgact gtcggcgact
     4801 ggtcgccgcg gttcacggtc agtgccaact ggagcatctc actgtcgaga tcgggagaga
     4861 tacagaaggc cgccatcaga gtggaacagc ggcagcgggc cctggatgag gcgactaccg
     4921 cagtcacgga ggccgttgcc aagaaccttg actacctgac gattgaggtc gcctccatgg
     4981 agaaatggcg caggcagctg gaggagtcag aagagtctca caggaaggcg ctggaatcct
     5041 acgaagaagg cgaagtcctt ctggttgatc tggaacgcgc aaggctgaat ttgcagaagg
     5101 cccgcaacaa ctacctggcc cattggggag ccgtatggga aatgtggtac accctggtcg
     5161 atatgagcat gcatgagtga caatccgata actacatgga ggcgattgag atgaagtttg
     5221 ggaataaggc attgatagtt ctgctgttgc ttccacttct cacgtttcgg gggttcggca
     5281 acacagcatc tgccgcagcg gcagcgagcg caaccacgcg gcttgaaatc ggcctggctc
     5341 agtgtgtggc gggctacgtt gctggatctg atgcggtgcg gaccgcggag aagaaagccg
     5401 cagactccgc agaatcgtac gaaagggcgg tggctgagaa gaaagccgct ctgtcactgt
     5461 gggagctgaa gatatgtgcc gaggcagcac agcttgcact ggccgaggca aggaacaatg
     5521 cggcactcct gggcgcccag gcctacatag ggcttgcaca ggcggtgcga gacgcgaaga
     5581 gtaagcatgc ctcgctcgcc gtggccgagg agaagctgag agtgctccgg ctgaggcacc
     5641 aggcgggtct gatcaccggc gataccgttc tgcagcagga gaatgcgtac ttatccgcaa
     5701 aggacgccgt catccgggcg gatgataccc taaagcaggc caagagcgat ctgtgtaggg
     5761 ccatggctgt tgatctgact gatgagatag tcctcaaaac agatgttgcg tcacttgccg
     5821 acgagagtgt gacgtatgat gccgccggtt gcatgaaact ggcgcgggca gcaagttcgt
     5881 cgtacttctc ggccgtgaag tcggaggaac tggccaggca gaagcaccaa gcgcttcagg
     5941 atccgatgat agccaccaag gctgaacgcg cctcggcaga agacgctttc cgcgatgccg
     6001 ccgacaagtt ggccaatgcg gagaagtctt tggacgattc agttgccaat gccctggctc
     6061 agctggactc gctgatccgc agtctacgca tgcaggttct atcctccgag ctttccgatg
     6121 ccaacctggg tgttgcgagg attaagttcg gatacggcga aatgctccag tacgaactgg
     6181 acagtgaagt taactcggtt agccaggctc atgacaaggt aaccaaggca agggaagacc
     6241 tgtatgttca ggtgctcaag atagagtcga tgatcgggcg cgatgtagtc gacatgctgc
     6301 gtcaggcggg tcttaagtaa ccgcctgagg taggcgcccg acaggccgct cctatggaac
     6361 gggtgaattg agttggtgaa gatacttctg gcggatgatg aacccaggat gaggagactg
     6421 gtgtcggact tcctgaagag agaaggttac gcgctggtgg aagccgaaga cggacagcag
     6481 gcgctcgacc tcttcttcag cgagaaggga attggcctgg tcatcctgga tgtcatgatg
     6541 ccgcgactcg acggttgggt cgtatgcaga gaaatacgaa aggcatccaa cgtgcccgtg
     6601 atcatgctta cggccagaag tgaggagagc gaccagctgt ttgggctgga catcggcgcc
     6661 gatgagtaca ttaccaaacc attcagcccc atgatattgg tagcccgggt caaagccctt
     6721 ctcaggagag ccggcggaga cactcgacgc cccaggctgt tcggacggct tgagatagac
     6781 agtgccaggc gtctggcgag tgtggatgga gagagagtct acctgagccc gaaggagtat
     6841 gaactgcttg tctacttcac ggacaatgaa gggatggctc tttctaggga gcagatcttg
     6901 agctctgttt gggactacga ctacttcggc gatactcgga cagtcgacac acatgtcaag
     6961 agactccgcg cgaaactggg cgacgcggca gagtacatcc agactgtccg cagcgtcgga
     7021 tacagattcg aggcgcttca atgatcaagt cggtgagagg gaagctcttt gcagggatag
     7081 tcggcctgat agcactgttc gtcctcttgt catgggccct caactggggt ctgctcgatg
     7141 gatactaccg aagtgccaag cgtggctctc tggtcaatgc gtatctcaag ttacgcaagc
     7201 tctaccaggg agaggccgga gcgcttctgc tggaactgga gaccatggag cggcgcgaag
     7261 ggttccatat cgtagtggtc gacccatggc agtcagtcaa gtacgacacc gtgcccgaca
     7321 ggcgcggagg gagcgcgcca acccccgatc gactcccgcg ccccggcagc gatcgatggc
     7381 tgaccgaatt catcgtgcgg accgcacagg atatggacgg ggattacgtt gttctcgagg
     7441 cgactccgga cgagaggctg aggaccgagt tcatgagcct cgtcggcctc ctggcaaatg
     7501 gggactacct ctttctgcga acgccggtca ctgctattca ggagagcgct gctactgcca
     7561 atcggttctt cttcatcagc ggatcgctga tgataatcgt aggcatcgta ttcgctttcg
     7621 tgtttgccgg gtggttcacg cgacccattc tggagatgaa gggtattgcc gagaaaatgg
     7681 cgcgactcga cttcagcagc aaatacagag gcagcagcca tgatgagata ggcgcgttgg
     7741 gagccagcat caacttgatg tccgaccagc tcgagcgctc aatcggcgaa ctggtgacgg
     7801 ccaatgagaa gcttctggaa gacatcgaga gagagcgcag aatcgatgac atgcgaaagg
     7861 agttcatcgc caacgtctcc cacgagctaa agacgcctat cgctatgatc caggggtacg
     7921 ccgaaggact caagctcaat gtgattgaag atgaggagaa caggaacttc tattgcgatg
     7981 tgatagctga tgaagccgcc aggatgaaca ggctggtcag ccagttgctc agccttgcta
     8041 ggatcgagtc cggaaatgcg gctcccgaca tgatctgttt cgatatcgtg gagatggccc
     8101 gcagggtgct ccggaagagc tgtctggtgc tgcaggagcg ggggattgac gcatcgaccg
     8161 agggcgaaga gcgcgcgctg gtcagtgccg attacgacat gattgagcag gtaatgacca
     8221 actatattac gaacgccatc aaccatgcgg ccggcgacaa catgctcaga gtgcgaatcg
     8281 acagcatacc cggcaaggta agggtatcgg tcctgaacag cggagaacca atacctttgg
     8341 agagtatcga ctcaatatgg actagcttct acaaaacaga caaggcgcgc actcgagaac
     8401 acggcgggac cggtctcggg ctgagcatag tcaaggccat tcaggacgct cacgggaatg
     8461 gatacggagc gtccaacgtc gacggcggag tagagttctg gttcgacgtc aatgcggcgg
     8521 ccgagactgg tctgctcgaa accgatgtgc ccgggactga tctgcccgaa actgatcttg
     8581 acatgagccc atcgaggtag taacatcttt tgtggacacc gcatcgcgtt gttcgcgatg
     8641 tgcccgggtg gcggaactgg cagacgcgca cgtttgaggg gcgtgtggtt ttaccgtacg
     8701 agttcaagtc tcgtcccggg caccattttt tcgaagtgtt atggcccgtg tgtggcaggc
     8761 ggcagcccgt cggcacatgg gtctttttcg tccaccgtca gttcataccc ggtcaaatcc
     8821 ccggtcaaat cgccacactt cggcctatcg aataagctag tgtgtgaaac acccgcgccc
     8881 gcgtcccggg ggagaacgcg ggccgcccaa ggagtggtga tcactgtgaa gatcgggctt
     8941 gcgctgggct cggggggcct ccgcggcgct gcgcacatcg gagtgatcga tgagctcgaa
     9001 aaggccgggg tgcgcgccga catggtggcc ggcaccagct cgggaagtct gattgcggct
     9061 atgtacgcaa gcggcatgac gccttctgag atggaagacg cggccctcgg catcacggcc
     9121 agagacatcc tggatcccat cggctacact gccttctttc tggtcgcgcc cgcctttatg
     9181 ctgctgctcg gcgccaaatc gtacctgccc aagggcatac ttcgcggtcg gcggttcgaa
     9241 cgtcttctga cctcgtattt cggcgagatg tccatgagcg acctgaagat gccttgctca
     9301 gctgtttcgg tagacattca cacgggcgag aagatagtgt tcacctcgga gagcccagca
     9361 cgcgccgcta tcttcaagga gaccgtgtac ttcaatgcgc cggtggtgga tgccgtgagg
     9421 gcatcctgtt caataccggg cgtgttcgag tggaaagcct ggggagggcg atacctcgtg
     9481 gacggcgccg ttcgcgagcc cgtgcccgcc aggatcctga aggaacaggg ctgcgactac
     9541 gtaatcgcag ttgacctcgg ctacacgggg caggccgatg cccaggtgcg cgagcttccg
     9601 tcaatcatca ctcagtctct ggatatcctg ggcgaggaag tgtccgacta cgtgctatac
     9661 tattatgcca acactatcat aaggccgcgc ctgtacaacg ttgccttgag cgacacttcg
     9721 cgcattccag aatgcataga ggagggacgc aacgcggcca ggcaggccct acccgatctg
     9781 cgccgcagta tccgccggag ggttatgagg cgcctcgcag cgatatagcg catgcattca
     9841 tcggatattg ccgctggcac ttgaaggaag ccttcgcgat gtcggcgaat atacaaacgg
     9901 atatacactt gtcagtcggg aaaggggaca agtcatggat accaagcaag cgctgatgca
     9961 tcttgccgat gcgcgcggcg tgaccgggta tgaatcccct gcctgggacg ttgtgaacgg
    10021 tctcttcgcc gacttagtcg acgaaatcag gcgggattcg ctcggcagtg caatcatgct
    10081 gaagaggggg gccgccaaag agccgcctgc cagcatcatg tttgcggcgc atctggacga
    10141 gatcggcatg attgtgtcga agatcgagga tgagggcttt ctacgcatct ggatgatggg
    10201 cggagtggac cgtcgcatac ttccgtctat ggaggtaacg gtgcacggac gccgagacct
    10261 ccccggcgtc ataagtgcga taccgcctca tcttcaggat ccgtccaaga aggacaagtc
    10321 ctggaagtgg gatgagctct tggtcgactg cgggatgccc gccgacaggc tgcgcgagct
    10381 tgtgcgagtg ggcgatgtga tcagcttcga ccgtcgggcg attgggcttg ccaacgaccg
    10441 aatcgccggc aagtctatgg atgaccgcgc cggcatcgcc gtcatgtacg aggcagcccg
    10501 cgagctgcag aagctatcgc atgagtgcga tgtgtatttc gtggccacgg tacaagagga
    10561 agtgggctac ggtggcagta tcaccagcac ttttggtgta atgcccgaca tcggaatagc
    10621 catcgatgtg ggccatgcgg ccatgcccgg cctgcccgag cacaaggtgc taaagatggg
    10681 cgggggtccg gccatcagct tcgggcctca tgtccacccg catatcttcc agaaactcaa
    10741 agagaccgct gaggaccgcg gcatcaagta tcagctggag ccgggcacta atccgcgcgg
    10801 cacggacgcc tacgcgatgc agatgacccg ttcgggggtg gccacagggt tgatctccgt
    10861 gcccttgcgc tatatgcata cctcagtgga aacgctcgcc ctcgacgatg tgaagaagtc
    10921 cgggcagctc ctggcatatt tcgccagcgc agtcgactcc cggttcaggg ggggattgac
    10981 atgcttctag ccaagctttc ggaggcgggc gggatttccg ggcaggaagc cgaagtgcgg
    11041 gcgctcatac gctccgagat aacgccttac gttgacgaaa tcaaggtaga ccccatcggc
    11101 aacctcattg cggtaaagcg cggacgcaag ggaaacaggc gcgtgatgct cgcggcccat
    11161 atggacgagg tcggcctgat ggtggtgggc atcgattctg acgggctgtt gcatgtagag
    11221 gctgcgggcg gggtcgaccc gagagtgctc gtgtccaagc tcgtatatgt gggcgccgac
    11281 cgccgccccg gggtgattgg gtccaagccc attcatcttc tcaaagccga agacatggac
    11341 cgcgctaccg acatggactc gctttacgtt gacatcggcg ccaagaacaa ggatgacgcc
    11401 gagaagctgg tgaagctggg cgaccagatc atcttcgcaa cacggtgcga acctctgggc
    11461 gaggggttcc tgaaaggcaa ggccatggac gacagggtgg gctgttacgc catcatcgag
    11521 gcgctcaaga aacagcatga cttcgacctt atcggggcct ttacggttca ggaagaggtc
    11581 ggcctgcgag gcgccggtgt ggcggcgtat caggtgcatc ccgacctggc gctagtgctt
    11641 gagggcacca cggctcacga cgttactgag acgcctgagc acggattctc cacggttccc
    11701 gggcgggggc ctgccatagt ctaccgcgat gcatcgtggg tgggcgatcg gcgaatcgtt
    11761 gatcgcttga tcgaaacggc ggaagctgct ggagtccctt accagatcaa acgcacagtt
    11821 accggcggca ccgacgcggg caggataggg ccgacggagt cgggagtccc cgtggcggtc
    11881 atgtcgatgc catgccggta catacattcc cccgtgtcgg tgatatgcgc tgatgacctc
    11941 aacaactacg tgaagcttgc cgggctgttc cttgacagcc ttgacgaaag gggtcttcca
    12001 gtatgaaaga tcttgcgaag aagctatctg aaagctttgg cccgtcaggg tcggaggagc
    12061 agataaggag catcatcagg tcggaagtgg agcagtacgc cgatgagata cgcgtagatg
    12121 ctatgggcaa cctgatatgc cgagtggcgc cgaaaaaccc agtgcctgga tcaggtgccg
    12181 gcgccccgcc caagcgcatc atggtggatg cgcacatgga cgagatcggc atagttgtgt
    12241 cgttcataga cgacaagggc ttcctgcgtt tctcgcccgt gggtgggctc agcccccacg
    12301 tgttgctggg ccagaaggta gtgttcgcag acggcacggt gggcgtattc ggcactgaga
    12361 aactcgacga catcaaggac ctcaagttcg acaagatgtt catcgatatc ggcgcgcgcg
    12421 accgggctga agcacaggcc aaggtgcgcg tgggcgacgc tgccgcgatg gtttcgagtt
    12481 tcgccgacat gggcgtgcgt gttagcggta aggccatgga tgacaggatc gcgtgcttgg
    12541 tactgatcga ggccatgcgg cgggcgcgac cccaggtgca tgaggcctac tatgtctttg
    12601 ctgtgcagga ggagctcggg ctgcgggggg cccgcacggc ggcctacggg atcaatcctg
    12661 acgtgggcat agcagttgat gtcactttgt ggggcgatac cccggagcac gctccgttca
    12721 acgttgcgct gggaggcggc gccgccataa aggccaagga ctcaagcctg attgcacatc
    12781 cggcagtcaa gaatctcatg atagacgtcg ccgagaagaa cggcatccct taccagcttg
    12841 aggtgttgga gaggggcggc accgatgccg gagcgatcgc gctcacccgt gagggagtgc
    12901 cgtccggaac catctcggtt ccgtgcaggt acgtgcatac gcccgccgag atggtcgaca
    12961 tgggcgatgt ggatgcctgt gtcaggctgc ttgtggccat ggtcgaagag ttggcatcgt
    13021 caaccggcgg tggtatactc tagacgaaaa ggcatctcga aatccggaag ctacggagga
    13081 atgatggtga gcaccgatac caggaaagag actaaacatg tcaagacaat catagccgcc
    13141 gacctcaagg gaaccgcggc caccgggggc tgccgggagt gccagacctc ctgccagtcc
    13201 gcgtgcaaga cctcgtgcac ccttgggaac caggcgtgca ggaagtagct cggcgcaccc
    13261 tttccgagca agggcgcccg tctccaaccg ggatacgccg caactggcac cggttcatgg
    13321 cagcgggcgc ccgtctcctt ttggacgtcc agtctggcag cttgcattca ctggatgagg
    13381 cggcgtggga cgttctggag gcaatggaac agggtaaggc cgtaggccag atagatggcg
    13441 ctgacgatgt tattgcggat cttgatgtcc tgatcgcgga gggtacgctt tcgcatcctg
    13501 acgacgcgga tccgatgccc gctgcactcg ggcccggtgt tgtcaaggcc atgtgtctca
    13561 atgtgtcgca cgactgcaac atgagatgcg gctattgctt tgccggcacg ggcggcttcg
    13621 gcggggagcg ttcactcatg ccggcggagg ttggctgcag ggcgctggac ctgttattcg
    13681 atatcagcgg acacgtccgc catctggatg tcgatttctt cgggggcgag ccgctgctta
    13741 acatggatgc ggtggccgag ataacccgtc atgggcgaga gcttgaggca ctcaccggca
    13801 agactctcaa gcttaccctc acgacgaact gctacgagct ttcgccggag acggacaggt
    13861 tcctgaacga caatgacatc gccgcggtgc tcagcgtcga cggaagatcc ggtattcacg
    13921 acggagtgag acggaccgcc gatggctgtc cgacttttga gcgaattgcc gacaatgcgc
    13981 tgcgtttcgt caatactcgc ccgaccggcg aatactacgt gcgcggcacc tatacccggc
    14041 gtaatctgga ttttgtgcaa gacgtccttt tcctggccga catgggcttc accagcatct
    14101 cgttcgagcc ggtcgtggct gcgccgtcgt cgccatactc catacgcgag caggacctgc
    14161 ctcgtgtgtt cgactcatac gaccggctgg tgacggagat ggccgcgcgc cgcgcagccg
    14221 ggcacgagtt caggttcttc catttcgacc tgaatgtcgc gcatggccca tgcctggcca
    14281 agcgccttct ggggtgcggc gccgggcgcg agtatgtagc cgtcactccc gacggcgcgc
    14341 tctacccatg ccaccagttc gtgggcaggg aagggttcat tatgggcaat gtgtgggacg
    14401 ggatcaccag gcctgagata ggtgaggctt tcgccggcgc ccacatctac gccaagagcg
    14461 cctgccgcga ttgctgggcc aggtacatgt gctccggtgg gtgccatgcc aatgcccacg
    14521 agatgaatgg cactctgatg actccgtact ccatcggttg cctcattacc cgcaagcgac
    14581 tggaggcggc gttggcgtac gcggccttgc agggatcttc gcagggctct tcacctgtat
    14641 gacgacagcc cgaccgccgg cgtcttccgc gaacctgtgc cctgtgggcc agggtttccg
    14701 aggggcctgg gtaaaccgac
//
LOCUS       DAOTAC010000021        14645 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_3463_length_14645_cov_4.712817, whole genome shotgun sequence.
ACCESSION   DAOTAC010000021 DAOTAC010000000
VERSION     DAOTAC010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 14645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14645
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3463_length_14645_cov_4.712817"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..928
                     /locus_tag="PLM74_01785"
     CDS             152..928
                     /locus_tag="PLM74_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41846.1"
                     /translation="MSVMRHICYAIPCYATADKRLSSTALRLRLAMENQPPLSVPEMS
                     KLVNRCVRTVRDYAERLIDAGWAVELDSDESGTRRIAASMPGHVEEFIAERISDGKWQ
                     VADAGQYLMLCVLDVGINSLSYLDNARPAFLRNVLTRRKMEYDRYYYAHKAAFEFNGP
                     QHYRTTQRYPDPDQLAKQQARDNMKAGISVRQGITLVEVRAEDLSFDHVMRRAPANLP
                     RRYVNPEGPLIQTVTSLCEEYVSYWIAKNAQGSAGDKDEG"
     gene            complement(1409..4480)
                     /locus_tag="PLM74_01790"
     CDS             complement(1409..4480)
                     /locus_tag="PLM74_01790"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HON41847.1"
                     /translation="MRPIRLAMQAFGPYPGQEVVDFRSLGNRRFFLIHGPTGSGKTTV
                     LDGICYALYGKSSGAERDVREMRSHHSPDDLPTQVVLDFSVGRESYRVWRRPEQEAPG
                     RRTPLKGDATLWQRTGISDDSVDGSVMETGLMRVTNNIEELLGFEADQFRQVVLLPQG
                     QFREALTADSAKRQQILEILFGTEVYRYIELALKDKAAELADALKSLKDRRETLLSAA
                     DAESQEQLQMAFEAERAMLGQARKVEKAASEAADAAAQALSKMRQDAEKLNEARAAAQ
                     AVAGLELQVAEHDAMERRCRKARLAAGCAGAEALLDEARREERARREAAGQTREELAR
                     AELAVRAASERLARENSPERAEQRKQLGAMLVTLEGFVPKVAEIDKAREAVIEASTAD
                     AKARGSASAAAARKSEIWSELAKARVEWNEAQAGGAELPLWESRVNEISTALKRAERR
                     AQLEADVAEKAIVAQRAQQSVDQAAEQLEQARRRVALLREAWLAGQAAALAAALEPGA
                     PCPVCGSTSHPSPAHAASAEDGGKAPSQKDLRSADMAVTIAESEWSRLSKAASEALQQ
                     EASVRGQLAELTSDGCDSRNLRVELRDAEKRLEAAETASQRTRQLDEMIGRLEQQFTA
                     AELEAAEAETASREVAGQLAAAKATVEEKELGVPVNLRSPAALEAEQVRVGTELDELV
                     KALEATERANEDAGRVLAGAQARAAATAEALEAAIVNVDARSLEFEAKVGEAGFASVD
                     EYHSSKMADDAARRLEQAIEQFRVQLHAARERLARAEGEATGIAEPDLAAAEARHSEL
                     DLAAKKAMENRIGLGNRIDQMRRQLDDLDKLAREMQEVEAEHTVIARVSDVANGRGHR
                     NRLGMTFERFVLASMLDQVTEAATQRLQVMSRGRYALRRTMDRLTSRSAAGLGLEVFD
                     AYTGRERSVTTLSGGEGFMASLSLALGLADVVQSYAGGIRLDTIFVDEGFGTLDPESL
                     DLAIDTLVELQREGGRLVGVISHVPELVERIDARLEISMTDRGSKSRFVVG"
     gene            complement(4477..5652)
                     /locus_tag="PLM74_01795"
     CDS             complement(4477..5652)
                     /locus_tag="PLM74_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HON41848.1"
                     /translation="MKLIHTSDWHLGRIFYKTHLTEDQAYILDQFIDIVRDERPDVVL
                     IAGDVYDRSTPPVEAVELLDDTLSRIVLGLGIPTIVIAGNHDSPERLSFGSSLMQSKG
                     LHIVGLPEFCSPIGMADESGPVWFVPLPYAEPAVVRDRLGAGEAKDHDASMAAQVNAA
                     FHGAERAAQREDRVVAVAHAYVAGGQQSESERPLTLGGTGAVDIGHFASFSYVAMGHL
                     HRPQTLAGSRVRYSGSLMKYSFDEADHAKSVTVADIDGAGEAVVRTVLLTPRRDVRRV
                     EGTFEEVVKGAASGVNREDYILACLSDPDPVWDCMNRLRQVYPNVMQVEWSAGMAGGG
                     SPGVLAGAGVDHRSRTELELFCEFWEQVTGDLIGADETAAVVALLEQFKAGREAAAS"
     gene            5884..6411
                     /locus_tag="PLM74_01800"
     CDS             5884..6411
                     /locus_tag="PLM74_01800"
                     /inference="COORDINATES: protein motif:HMM:NF019099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HON41849.1"
                     /translation="MKPGKLQRQIQLTMLVAVALALHVVESLLPPLPVPGAKVGLANV
                     ATLVCLRYMGFWPAVGVSAVRSILGSVIAGKFWGLGFWMSFVGATASALGMGLSLRLL
                     PSEASMVWTSVVGGVVHASCQLTIAVLAMRQPALFFGAPLLLGLSIITAVFIGLVCDN
                     LDGPARRFLYAAKRR"
     gene            6485..6844
                     /locus_tag="PLM74_01805"
     CDS             6485..6844
                     /locus_tag="PLM74_01805"
                     /inference="COORDINATES: protein motif:HMM:NF018685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HON41850.1"
                     /translation="MTKRRIMPAILMLLLGAILIFAGVRGRQHAPKGSVLTVTSIGEA
                     QNRVVAEVTLSPTAKRTIQVEGPLGITVVEVEGTRAHVVSSPCPDKICIRMGWLQRAG
                     DYSACLPNRVLVEVGDE"
     gene            complement(6946..8034)
                     /locus_tag="PLM74_01810"
     CDS             complement(6946..8034)
                     /locus_tag="PLM74_01810"
                     /inference="COORDINATES: protein motif:HMM:TIGR01883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HON41851.1"
                     /translation="MDSIVDEFVELVKIDAESGSEAQMAAAVAGKLRGIGLEVEQDNA
                     GNVIGRLSAHGSGCSCGDAIMLCAHLDRVPPGKGVNPVIRDGVVYSSGDTVLAADDIA
                     GVTAILAGLRMAKASGKCLPPVEVVFTVSEERGLRGAKQLDYSRLASKTGYIFDAADP
                     VGTVILSSPTHMGLEVQITGRAAHAAANPEDGISAIQVAAEAIVEFPFGRVDAQTTAN
                     VGIMSGGTATNIISEKAYFKAEVRSLDHQRALGLAEECAAKIRSIVTRRNARADIATT
                     VDYCGYTIPKDAPVVERAVRALRTAGRDAVFQSTMGGSDGNVFNAHGIACVVLGIGSR
                     DVHSTRESQPIGELVAAAELARDIILVG"
     gene            complement(8085..9059)
                     /gene="rapZ"
                     /locus_tag="PLM74_01815"
     CDS             complement(8085..9059)
                     /gene="rapZ"
                     /locus_tag="PLM74_01815"
                     /inference="COORDINATES: protein motif:HMM:NF015620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HON41852.1"
                     /translation="MINISGSDVSFVIITGLSGAGKTQSMKALEDLDFFCIDNLPPSL
                     LPQLVELHADAAEHRKYAVAMDVRGKEYFGHLVDALNWLARSEYHYTVVFLECRDSVL
                     VRRFSETRRQHPLSGAGSIYDSIALERRVLAPIRERAHIVLDTSDLKPVELQAHLHAA
                     VMGTPVHEMMVVDVFSFGFKYGAPIDADIVFDVRFLPNPFYVRDLRPTTGLEPACAAY
                     VMDSPIARSFMETFRDLILSLIPYYSKEGKGRLTIGIGCTGGQHRSVAVAEELGRQLA
                     QRGVRSQVRHREITDRKLAVDSGLLQDDMGDNTGNNTGNNTGDNAGDV"
     gene            complement(9323..9520)
                     /locus_tag="PLM74_01820"
     CDS             complement(9323..9520)
                     /locus_tag="PLM74_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41853.1"
                     /translation="MNRSVQRMSVEQAVFMAGNNPDLAAGLIGFNERGAYMERREYLQ
                     AAETVRIARAAAIGRPAVQRG"
     gene            complement(9535..9915)
                     /locus_tag="PLM74_01825"
     CDS             complement(9535..9915)
                     /locus_tag="PLM74_01825"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HON41854.1"
                     /translation="MSGTRVIVADDRREVRYGLRVLLEEEQARCVVMEAADLTQLMSQ
                     VREPCADLVLLDWELGPVPGSDILAMLKGVCPKTRVVALSTRPEARNSALSSGATAFV
                     LKVDCVESVLTAVRSAMASSREDR"
     gene            complement(10186..10809)
                     /locus_tag="PLM74_01830"
     CDS             complement(10186..10809)
                     /locus_tag="PLM74_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HON41855.1"
                     /translation="MIVDDHAMVRSGLRAFLSVYEDLEFAGEASDGREAVEMCSVIRP
                     DVVLMDLVMPDVDGASATRAIRKVCPNIQVIALTSFKEQDLVQGALQAGAIGYLLKNV
                     SADELASAIRAAHAGRPTLAPEATEALIRASIQAPPEPEIGGDLTDREREVLAALAQG
                     LTNPEIADRLYISRATVKFHVSSILSKLGVQTRTEAVALALQKNLIR"
     gene            complement(10833..13421)
                     /locus_tag="PLM74_01835"
     CDS             complement(10833..13421)
                     /locus_tag="PLM74_01835"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HON41856.1"
                     /translation="MPDDSQDVRMVTSMARFDRDLALVDFGDDWAKIISRVAKIPLER
                     IVRGASLPEIAGDEGRDWVGRYRDALDGRSDYSRVALRGEAGGGPAGATATSWDLYAL
                     PLTLNEQIVGVVELAVRADRTVEAAILASLPDMCFVLSYDGIVAGCHGSSLPEIFGGP
                     DKLIGRPFAEIAPESERRRIRAALDEVARGAQAGDEAGRAGGGDSKIETATSLEFVVG
                     RGVSAQVFEARFAPLPSGGTIVVVRDVTEAKRALAIQSGQNRFLELLARGGAFEETLT
                     SLIRILEEQTPGMSGLILLLDADGRRLRIGASATLPREYVDSLDGLVIGPAVGSCGTA
                     AFTGERVIVEDIMTDRRWAGLRDLAAKYGLRACWSQPVVDDDGAVMGTFAMYYRTPRA
                     PLAVELKAIETAARLVRVAIEQRHAREEVEAAYEQLERRIEERTREIERRRRVAEGLR
                     EILGSLNSARSLEDILDLILEQAEEFLGAEAGALYRLHADGDMLDVAAARGLAEASCG
                     PIAVGEGVVGWAVMAGRPVVVSGVGTSGRQKADRPPANRAPVGRGRAGRGNAAGRVQP
                     EARLDRSQWDELPDWALGRFRSLMAVPLVGKDQVYGVIALCFSESRGFSHEELKLAMS
                     FGDQAALAVENAELRAKAEESAAAAERSRLARDLHDAVTQTLFSASLIAEVLPRLWKQ
                     NEEEGLRRLDELRRLTRGALAEMRSLLLELRPATLMEVGLEELLRQLAEAMMGRAGIP
                     VHVELSGASCAGTCALPPQVRVGLYRIAQESLSNVVKHSGAREAWVSLTCSTDQSGAI
                     RIQLRIRDNGRGFDQDVVSPEHLGLRIMQERAAAIGARISINSTVGQGTEIVVAWSGL
                     RGGEGS"
     gene            complement(13473..>14645)
                     /locus_tag="PLM74_01840"
     CDS             complement(13473..>14645)
                     /locus_tag="PLM74_01840"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012448241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HON41857.1"
                     /translation="GDEAYAGSENWLHLEETVRELFGFKYVCPTHQGRGAENLISQIL
                     VRPGTYVPGNMYFTTTRAHQEMNGGTFVDVIIDEAHDPQLDSVPFKGDVDLAKYEALI
                     KKVGADKIPYISVAVTVNMAGGQPVSMANLKAVYEMSKKYGIRVIFDATRCVENAYFI
                     KKREPGYENRTIASIVHEMFSYSDGCTMSGKKDGIVNMGGFLATNEEEVYRQATSRVV
                     VYEGMPSYGGMTGRDMEAFARGLREAMDYAYIEHRVEQVGYLGAKLIEAGVPIVRPVG
                     GHAVFLDARAFLPHIPQPKFPAQALAAAIYEESGVRSMERGIISAGRDKEGKDYQPKL
                     ELVRLTIPRRVYTNAHFDYVADSIAEVYARRDQVRGLKWVYEPPVLRFFTGRFEQE"
BASE COUNT         2760 a         4875 c         3966 g         3044 t
ORIGIN      
        1 cccatgggcc tggcggtcgc acccgacatc gaagcctgaa cacagccctt gcacatcctg
       61 ctccaaatcc cccgaacggg cgataccaac ggaagcctca agatgatacc atcgtcatgc
      121 cgacgaagac tgctagaaga ggatgagatg agtgtccgtg atgcgccaca tttgctatgc
      181 cattccttgt tacgcaactg ccgacaaaag gctcagttct actgctctcc ggctgcgcct
      241 ggccatggag aaccaaccgc ctctgtcggt gccggagatg tccaagctag tcaacagatg
      301 tgtccgcacc gtacgcgact acgcagaacg gctgatagac gcagggtggg cagtggaatt
      361 ggactccgat gaatccggaa cgcggcgcat tgccgcttca atgcccggtc acgtggagga
      421 gttcattgcc gagagaatca gtgatggaaa atggcaagta gctgatgcag gccagtacct
      481 catgctatgc gtcctagatg tcggcatcaa ctctctgagc tatctagata acgcccggcc
      541 cgcgttcctc cgcaacgttc tgacacggcg caagatggaa tacgaccgtt actactacgc
      601 ccacaaagca gcattcgagt tcaatggtcc acagcactac agaacgacgc agcgataccc
      661 ggacccagat cagctagcaa aacaacaggc cagagacaac atgaaggccg gtatcagtgt
      721 tcgacaaggt ataaccctgg tagaggtaag ggccgaagac ctctccttcg accatgtgat
      781 gcgcagagcc cccgctaatc taccgcgaag atacgtgaac ccggaaggcc ctctgatcca
      841 aaccgtaacc agcctgtgtg aggagtacgt atcgtattgg atagcgaaga acgctcaagg
      901 cagcgccggc gacaaggatg aagggtagcc caaggacatc ccgaggcccg aaggagaaca
      961 gtcggagctt ggtcggccgg gcagcgtgcc tttttatagc attcggcaga cacttacagg
     1021 tttctgcaag gagaagcccc gagtacggag attttccgcc gcgatgcggc ggaaaatttc
     1081 ctttctgccc cgttttcgcg gccgggagcc aagtcgactg cggctatgtc tatatctgtc
     1141 aggttttttc tagggcttct tgactaagat tccctgtttc ctgctttcct cccctttggc
     1201 acatgtctag ctaatctgat gctggcagtt acaggttctc atatgggtct ctatggggat
     1261 tcgtgatggc gtgcggagat ttttcgcacg tgcgctgaag aaaatctccg caaggtgccc
     1321 cgatgtgtac aaaaacctgt aaatgacagc gacatctcat ggtttgggct caccgtcgcc
     1381 gggcgatccc attattgcac cgtcgcgcct accctaccac gaaacgcgac ttgctgccgc
     1441 ggtcggtcat gctaatctcg aggcgggcgt ctatccgctc tacaagttca ggcacgtggg
     1501 agatgacgcc taccagccgg ccgccttctc gttgcagctc cacaagggtg tcaatggcca
     1561 ggtcgagcga ttcgggatcg agtgtgccga atccctcgtc aacgaagatg gtatcgagcc
     1621 taatgccccc ggcgtatgac tgaaccacgt ccgccaggcc cagagcaagc gatagcgacg
     1681 ccatgaaacc ttcaccgcct gacagggtgg ttacgctgcg ctctcggcca gtgtaggcat
     1741 caaacacttc caggccgagc cccgccgcgc tccgagatgt cagccgatcc atggtacgcc
     1801 tcagagcgta cctgcctctg ctcataacct gcaaacgctg ggtggcggcc tctgtcacct
     1861 gatcaagcat agacgcaagc acgaaccgct cgaaggtcat ccccagcctg ttccgatgtc
     1921 ctctcccatt ggctacatcc gacactcgag cgatcaccgt gtgttcggct tcgacttcct
     1981 gcatctcgcg agcaagcttg tccagatcat ccagctgtct gcgcatctgg tctatccggt
     2041 tgcccaagcc tatccgattc tccatcgcct tcttggcggc cagatccaac tcactgtggc
     2101 gagcttcggc cgccgctaga tccggctccg cgatcccggt ggcttcgccc tccgccctcg
     2161 ccaggcgctc cctggcagca tgcagctgca cacggaactg ctctattgcc tgttccaacc
     2221 tacgagcggc gtcgtcggcc attttcgagg agtgatactc atccacgctg gcaaaccctg
     2281 cctcgcccac cttggcctca aactccaagc ttcgagcatc cacgttcaca atcgcagctt
     2341 caagcgcttc ggccgtcgca gccgcacgtg cctgcgcccc cgccaggacg cgcccggcat
     2401 cttcatttgc gcgctcggtt gcctccaaag ccttcaccaa ctcgtccagc tcggttccga
     2461 ctctgacttg ttcagcttcg agcgcggcgg gggacctgag gttcaccgga acaccaagtt
     2521 ccttctcttc cactgtcgcc ttagccgccg ctagctgacc tgccacctca cgactggcag
     2581 tctccgcttc cgctgcctca agctcggccg ctgtgaactg ctgctcaagc cgcccaatca
     2641 tttcatccag ctgccgagtg cgctgactcg cagtttctgc agcttcgagg cgcttttccg
     2701 catcccgcag ctcgacacgg aggttgcgac tatcgcagcc gtctgaggtc agctcagcaa
     2761 gctgacctcg caccgacgcc tcctgctgca atgcctcaga agcagcctta ctcaggcgcg
     2821 accactcgct ctccgctatc gtcaccgcca tatccgcgct tcgcaagtcc ttctgagaag
     2881 gcgccttccc accgtcttcc gcagacgctg cgtgagccgg cgacgggtgc gatgtggatc
     2941 cacataccgg gcacggcgcg cccggctcaa gcgccgccgc aagagccgca gcctggccag
     3001 caagccaagc ctccctaagg agagccaccc gcctccgcgc ctgctcaagc tgttcggcag
     3061 cttggtcaac tgattgctgc gcccgctgcg ccacgatcgc cttctccgcg acatcggctt
     3121 ccagctgcgc gcggcgctca gcccgtttca gtgctgtgct tatctcattg acgcgtgact
     3181 cccagagtgg cagctccgcc ccaccagcct gagcttcgtt ccattcgacg cgggctttgg
     3241 ccagctcgct ccatatctcg cttttccgcg cggctgcggc tgacgccgag cctcgcgcct
     3301 tcgcgtctgc cgtgctcgct tctatcactg cctccctggc cttatcgatc tcggctactt
     3361 tgggcacaaa gccctccaat gtcacaagca tggccccaag ctgcttccgc tgttcggcac
     3421 gttccggtga gttctcgcga gccaacctct ccgacgcagc gcgaaccgct aactcggccc
     3481 gagccagctc ctcccgggtc tgcccagccg cctcgcgcct ggctctctcc tccctacggg
     3541 cctcatcgag cagtgcttca gcccccgcgc aacccgccgc gagccttgcc ttcctgcacc
     3601 ttcgctccat cgcgtcatgc tccgccacct gcaactccaa accggcaaca gcctgcgctg
     3661 cagcgcgtgc ctcgttaagc ttttcggcat cttgcctcat tttggacaga gcctgtgctg
     3721 ccgcgtccgc cgcctcgctc gctgccttct cgaccttccg cgcttggccg agcatggcgc
     3781 gctcagcctc gaatgccatc tggagctgct cctgtgactc ggcgtccgcc gcgctaagga
     3841 gagtctcccg tcgatccttg aggctcttca atgcatcggc caactcggcc gctttgtctt
     3901 ttagtgccag ttctatatat cggtagactt cagtgccaaa taggatctcc agtatctgct
     3961 ggcgcttagc agaatcggcc gtaagagcct cccgaaactg cccttgcgga agcaggacca
     4021 cctggcggaa ctggtcggcc tcaaaaccaa gcagctcttc tatgttgttg gtaacccgca
     4081 tgagccctgt ctccatcacc gaaccgtcca ctgagtcgtc gcttatgcct gtccgttgcc
     4141 atagcgtagc gtctcctttg aggggagtgc gtctacccgg cgcttcttgc tcgggcctgc
     4201 gccacacacg atatgactcg cggcccacgg agaagtccaa taccacctgt gtcggaaggt
     4261 cgtcaggcga atgatgcgat ctcatctccc ggacgtcgcg ctcagcaccg ctggactttc
     4321 catatagagc gtagcaaatc ccgtcgagca ccgtggtctt gcccgagccc gtgggcccgt
     4381 gaatcaagaa gaaccgacgg ttgcccagac tgcggaagtc cacgacttct tgccctgggt
     4441 atggcccgaa ggcctgcatc gcaagacgta tgggtctcat gacgccgcgg cctcccttcc
     4501 tgccttgaac tgctccaaca gcgcgaccac agccgccgtc tcgtccgcgc ctatgagatc
     4561 gcccgtaacc tgttcccaga actcgcagaa caactcgagc tctgtacgac tgcggtgatc
     4621 aacacccgcc ccagcaagaa cgcccggact gcctcccccg gccattccgg cgctccactc
     4681 cacttgcatg acattggggt agacctgtcg cagcctgttc atgcaatccc atacaggatc
     4741 cggatcagat aggcacgcca atatgtagtc ttcgcgattc actccagacg ccgcgccttt
     4801 caccacctct tcgaacgtgc cttccaccct ccgaacatcc cgcctgggcg taaggagcac
     4861 ggtccttaca accgcctcgc ccgccccatc gatatcggcg acggtcaccg atttggcatg
     4921 gtcggcctcg tcgaatgagt acttcatgag cgaccctgaa taccgcaccc ggctgccggc
     4981 cagagtctgc ggccgatgta ggtgccccat agccacgtag ctgaaggaag cgaaatgccc
     5041 aatgtcgact gctcctgtgc ctcccagcgt gagtggtcgc tccgactctg actgctgacc
     5101 cccggcgacg taggcgtgag ccacggcgac aaccctgtct tcgcgctgcg ccgcgcgttc
     5161 ggccccgtgg aaagcggcat ttacctgggc ggccatagaa gcgtcatggt cttttgcctc
     5221 cccggctccc agcctgtcac ggacgactgc cggctccgcg tatggaaggg gcacgaacca
     5281 cacggggccg gattcgtcgg ccatcccgat agggctacag aattcgggta gcccgactat
     5341 gtgcaggccc ttgctctgca tcagagatga gccaaagctc agacgctccg gcgaatcgtg
     5401 attgccggcg atcacaatag tcggaatccc aaggcccaac acgattctgg agagtgtgtc
     5461 atctagcagt tcaacagcct ccaccggcgg cgtcgacctg tcgtatacat ctcccgcgat
     5521 cagcacaaca tcggggcgtt cgtcacggac aatgtcgatg aattgatcaa gtatgtacgc
     5581 ctggtcttcg gtgagatggg tcttgtagaa tatccgcccc aggtgccagt cggacgtatg
     5641 tatcagcttg atgcagaccg cctccttctc cgcaagcgct gccagattcc tgtccgattc
     5701 cttacatgag gattctcccc aaaagtggat ccttcctctc ggttatctgg tctttccaat
     5761 acggctcttc ggatgccctt cggatgcagt cgcgggcatc ggacacatag tgtataattc
     5821 ggtttgaact gcgcctgcgt ggcacctgta atctccgtgc atggcaagac agggggatcg
     5881 catttgaaac ccggtaaact gcaaagacag atccagctca ctatgcttgt ggcggtcgct
     5941 cttgccctgc acgtagttga atcactgctc cccccactgc cggttccggg cgccaaggta
     6001 ggactggcga atgtcgctac tttggtgtgc ctgcgctaca tgggtttctg gccagctgtc
     6061 ggcgtgagcg cggttcgcag catactcgga agcgttatcg ctggcaagtt ctggggcctt
     6121 gggttctgga tgagttttgt cggcgccact gcaagcgccc tgggcatggg cttgtccctc
     6181 cgcctgctgc cctccgaggc ttccatggtg tggacaagcg tggtgggcgg agtagtccac
     6241 gccagttgcc agctgaccat tgcagtcctg gcaatgcggc agccggcact gttttttggc
     6301 gctccattgc tgctcgggct gtcgataatc acggcagtct tcataggact ggtgtgcgat
     6361 aatctggatg gcccggccag gcgctttctt tatgccgcca aaagaaggta ggagtcactg
     6421 agggctctac tcagggcttt ctgagggctc tactgagtag tctgctgagg agtctactaa
     6481 gctgatgaca aagcgcagaa tcatgcctgc aatcctaatg ttgctgcttg gcgccattct
     6541 catcttcgca ggagtccggg gtcgccagca cgcgccgaag ggctcggtgt tgaccgttac
     6601 gtccatcggc gaggcccaga acagggtagt cgcggaagta acgctttcac ccaccgcgaa
     6661 acgaacgatc caggtcgaag gaccgctcgg catcacagta gtcgaggtcg aagggacgag
     6721 ggcacacgtg gtctcgtcgc cgtgccccga caagatatgc atccgcatgg ggtggctgca
     6781 gcgggcaggc gactacagcg catgcctgcc gaacagagtc ctcgtggaag taggagacga
     6841 atagcctacc ccgaacaacc cgacggaggc cgatccggcc gagccgaccc gacccgcatc
     6901 tagcgcgcga cggcccccgc cgctgctttc aacatctctc tccggctatc ccacaaggat
     6961 aatgtcgcgg gcaagctcag ccgctgccac caattcgcct atgggctggc tttcgcgagt
     7021 cgagtgaaca tcgcggcttc ctatgcccag tacaacgcag gcgattccgt gggcattgaa
     7081 cacgtttccg tcactgcctc ccatggtgga ctggaaaacg gcgtcgcgtc ctgcagttct
     7141 cagggccctg accgcacgtt caacaacggg agcgtctttc ggaatcgtgt atccacagta
     7201 atcgactgta gttgcgatat cggcccgcgc gttgcggcgg gtcactatac tgcgtatctt
     7261 ggccgcgcac tcttcggcaa gacctagcgc ccgctgatgg tcgaggctac gaacttcggc
     7321 cttgaagtaa gccttttcgg atatgatgtt ggtcgcagtt ccgcccgaca tgattcccac
     7381 attggcggtg gtctgtgcgt cgacgcggcc gaaggggaat tcgacaatgg cttcagctgc
     7441 cacctgtatc gcgcttatgc catcctccgg gttggctgcc gcgtgggctg cccgtccagt
     7501 gatctgcacc tcaagcccca tgtgtgtcgg gctcgacagg atcactgtgc cgacaggatc
     7561 ggcggcgtcg aatatgtaac ctgtctttga ggcgagtcgg gagtaatcca gctgctttgc
     7621 gccgcgaagg ccccgctcct cgctcacggt aaacaccact tcgaccggcg gtaggcactt
     7681 gcccgaggct ttcgccatgc ggaggcctgc caggatagcc gtcacgcccg ctatgtcgtc
     7741 agctgccaga acagtgtcgc cgctggagta gaccactccg tcgcgtatta cgggattcac
     7801 gcccttaccc ggcggcactc ggtcaaggtg cgcgcagagc atgattgcgt ccccgcagga
     7861 acacccggaa ccgtgagccg acagccgacc gatgacgttg cctgcgttgt cctgctcgac
     7921 ctcgagcccg atgcctcgca gtttgcctgc tacagccgcc gccatctggg cctcgcttcc
     7981 cgattccgcg tcgatcttga ccagctcaac aaactcatca acaatactat ccatacacga
     8041 tcaccgtctt tcccattgtc gctttcgcct atgccgtttg cgcctcatac atcgcccgcg
     8101 ttgtcacccg tgttgttgcc tgtattgttg cctgtattgt cgcccatgtc gtcctgcagc
     8161 aacccgctat cgactgctag cttgcggtcg gtgatctctc tatgccgcac ctggctcctg
     8221 acacctctct gtgccagttg ccgcccaagc tcctcggcta ccgctaccga ccggtgctgc
     8281 cctcctgtgc atccaatccc aatcgtaagg cgccctttgc cttctttgct gtaataaggt
     8341 atcaagctga gaatgaggtc acggaaggtc tccataaaag accgggcgat gggcgaatcc
     8401 atgacgtagg cagcgcacgc gggttctagg ccggtggttg gtctcagatc acgcacatag
     8461 aaaggattag gcagaaaccg cacatcgaat acgatatcgg cgtcaatcgg cgccccgtac
     8521 ttgaacccga aggaaaatac atccacaacc atcatctcgt ggacgggagt gcccatcact
     8581 gccgcatgca gatgcgcttg cagctcgacc ggtttcaggt ccgaggtatc gagcactatg
     8641 tgcgctcgct cccttatggg cgctaacacc cggcgctcca gcgctatact gtcgtatata
     8701 ctgcccgcgc ccgatagagg atgctgacgg cgcgtctcgc tgaacctccg cacaagcacc
     8761 gaatccctac actcaaggaa gaccactgtg taatggtact cgctgcgagc cagccaattc
     8821 agtgcatcca ctagatggcc gaagtactcc ttgccgcgaa catccatggc caccgcatac
     8881 ttgcgatgtt cggctgcgtc ggcatgcaat tcgaccagct ggggcaggag agacggtggc
     8941 agattgtcga tacagaaaaa atccaggtct tccagggcct tcatagactg ggtcttgcct
     9001 gcgccggaaa gcccggtgat tattacgaat gagacatcac tgccggatat gttgatcacc
     9061 ttccccgcgc cccctggcgg tgttgaattg cctgtttcga cgggcgatca cataatccct
     9121 ccatgcaata gcagaatccc cggccgttct atcttttgcg taagccccgg ccggggattc
     9181 atctcatgtc ctgtttgtgg agcagcccta tactacccgt ttccggtgtg tgcgtcactt
     9241 ggcgaatacc cattggctaa agcacctttc ctagtcatgt tttggcgcac cccctgtctg
     9301 tttcacctga ccctccgcgg catcagcccc tctgaacagc cggccgacca atcgctgcgg
     9361 cccgcgctat tctgaccgtc tcggcggcct gtaggtactc ccggcgctcc atatatgccc
     9421 cacgctcatt gaagccgatc aggccggctg ccaagtcagg gttattgcca gccatgaaca
     9481 ctgcctgctc cactgacatc cgctgtacac ttcggttcat ttcagtccct cctttcatcg
     9541 gtcttctctg gacgatgcca ttgccgatct gactgcagtg agcactgact caacacaatc
     9601 caccttgagc acgaacgcgg tcgcaccaga cgatagagcc gagtttctgg cctccggtcg
     9661 cgtgctgagc gccacaacac gggtctttgg gcatacgccc ttgagcattg ccaggatgtc
     9721 ggagcccggg acggggccta gttcccagtc gaggagcacc aagtcggcac agggctcccg
     9781 tacttgcgac atcagctgcg ttaggtctgc ggcttccatc acgacgcacc ttgcctgctc
     9841 ctcttcgagc aacacccgaa gcccgtatcg gacctcgcgc cgatcgtctg caactatcac
     9901 ccttgtgccg gacacgccat ctgcgccacc ctctgctgtc tgatctgctc ggcgcttcgg
     9961 tcggtcgagc gtgagcatcg cgccttgcaa ttcagagtct acacgtgatg cgacgtgggc
    10021 gaatcagccg actgtcggag aggccggata gacactggaa tggtttttcc ctggccatct
    10081 ggagaggagg agagaaggaa atgagtcagg cgcagatccc cgcctctcga cagcggaaac
    10141 ccacgcctgc gcgtaaactc cttgacacgt ggtgcctgag gcctgctatc tgattaggtt
    10201 tttctggagc gccagcgcca ctgcctcggt tcttgtttgt acgcccagct tggatagtat
    10261 gctgctcaca tggaacttga ccgtagcccg gctgatgtac aacctgtccg caatctccgg
    10321 gttagtaagg ccctgcgcca gggcggccag cacctcgcgc tcgcggtcgg tgaggtctcc
    10381 gccgatctcg ggctccggcg gcgcctggat ggaagccctt atgagagctt ctgtcgcttc
    10441 aggagcgagc gtgggccgcc ctgcatgagc agcgcggatc gccgatgcca gttcgtcggc
    10501 ggacacgttc ttcagcaagt acccgatggc gccggcctga agcgccccct gcaccaagtc
    10561 ctgctccttg aagctcgtaa gcgcaatgac ctgtatgttc gggcagacct tgcgaatcgc
    10621 cctggtggcc gaagcgccat ctacatcggg catcacgagg tccatgagca ccacatcagg
    10681 acggattacc gagcacatct ccaccgcttc cctgccgtcg ctcgcctctc cggcgaattc
    10741 caggtcttca tagacgctta ggaatgccct gagcccgctt ctcaccatgg catgatcgtc
    10801 cacgatcata acacggatta cggcagcacc gctcaactgc cttcccctcc tcgcaggccg
    10861 ctccaggcga ccacgatctc ggtcccctgg ccgacagtgc tattgataga gatgcgcgcc
    10921 cctatggccg ccgcccgctc ttgcattatc ctgaggccca agtgctcagg cgagaccaca
    10981 tcctggtcga acccccggcc gttgtcgcga atccgaagct ggatccgaat tgccccggac
    11041 tggtcggttg aacatgtcag actcacccag gcttcgcgag ctccggagtg tttcaccaca
    11101 ttgctcaggg attcctgcgc gatcctgtaa agccccaccc gaacctgcgg aggaagcgcg
    11161 catgtacctg cgcaggaggc gccactcagt tccacatgaa ccggtattcc tgcccggccc
    11221 atcatcgcct ctgcaagctg ccgcaggagt tcctccagcc cgacctccat aagcgtagcc
    11281 ggcctgagtt cgagaagaag cgacctcatc tcggccaaag cgcctcgggt tagccgcctc
    11341 agctcgtcca ggcgcctcag gccctcctct tcattctgct tccacagcct cggcaggact
    11401 tccgcgatga ggctggctga gaagagagtc tgggtgactg cgtcatgcag atccctggca
    11461 agacgactcc gttcggcagc cgccgccgat tcctcagcct tcgccctgag ctcagcattt
    11521 tctactgcca gagcggcctg gtcgccgaag gacatcgcca gcttcagctc ttcatgggaa
    11581 aacccgcgcg actcagagaa acatagcgcg atcacgccat atacctggtc cttgcccacc
    11641 agcggaaccg ccatcagact cctgaatcgc ccgagtgccc aatcgggcag ctcatcccat
    11701 tgcgatctgt caagccgcgc ctcaggttgc acgcgcccgg cggcattacc tcgcccagcg
    11761 cggcctctgc cgaccggtgc ccgattcgcg ggcgggcggt ccgccttctg tctacccgag
    11821 gtgccgactc ccgatacgac caccggtcgc ccggccatga ctgcccagcc cacgacgccc
    11881 tctcccacgg caatcgggcc gcagctcgcc tcggcgagcc cccgggctgc ggcgacgtcc
    11941 agcatgtcgc cgtctgcgtg gagccggtag agcgcgccgg cttcagcgcc caggaactct
    12001 tcggcctgct ccagtatgag atcgagtatg tcttccaaag acctagcgga gttgagagat
    12061 cccaggatct cgcgcagacc ctccgccact cggcgccgcc gctcgatctc acgcgtacgt
    12121 tcttcgatac ggcgctccag ctgctcgtat gctgcttcga cttcctctct ggcgtgcctc
    12181 tgctctattg caacacgcac cagccgggca gctgtctcaa ttgccttcaa ctccaccgcg
    12241 agcggagcgc gcggcgttct gtagtacatc gcgaatgtcc ccatcacagc cccgtcgtcg
    12301 tccacaacag gctgtgacca gcatgcgcga aggccatact tggctgccaa atccctcagg
    12361 ccagcccagc gtctgtcggt catgatgtct tctactataa cgcgctcccc tgtaaaagcg
    12421 gcagtaccgc acgagccaac agccggcccg atcaccagcc cgtccaggga gtccacatac
    12481 tccctcggca gtgtggcgct ggccccgatg cgcaaccgcc tcccgtctgc atcaagcaga
    12541 agtatcagcc ccgacatccc cggcgtttgc tcctcgagaa tgcgtatgag agaagtgagt
    12601 gtctcctcaa atgcgcctcc tctcgcgaga agctcaagga atctgttctg cccggactgg
    12661 atcgccaggg cccgcttggc ttctgttaca tctcggacaa ccactattgt gccgcccgaa
    12721 ggaagcggag cgaatcgcgc ctcaaagact tgagcgctta ccccgcgccc taccacgaac
    12781 tccaggctgg tagctgtttc gatcttcgag tctcccccgc cggctcgccc ggcttcgtca
    12841 cctgcttggg cgcctctggc tacttcgtcc agtgcggccc ggattcgccg acgttccgac
    12901 tccggcgcaa tctcggcaaa aggccttcca atgagcttgt cagggccccc gaagatttcc
    12961 ggcaggctgg acccgtggca tccggccaca atcccgtcgt agctcaacac gaaacacatg
    13021 tccggcaggg atgcaagaat cgccgcctcc acagtgcgat cggcacgcac cgcaagctca
    13081 accacgccca ctatctgctc attcagggtt agcggaaggg catacaggtc ccagctcgta
    13141 gcagttgcac ccgcaggccc gccgcccgcc tcgccccgca gggcgactcg ggagtaatca
    13201 gaccggccat cgagagcatc ccgatagcgt ccgacccaat cgcggccttc atcgccggct
    13261 atttcgggca ggctggcccc gcggactatg cgctccaggg gtatcttggc cactcgggat
    13321 atgatcttgg cccagtcatc gccgaagtca actagagcca agtcgcggtc gaacctggcc
    13381 atggacgtta ccatgcgcac gtcttgagag tcgtcaggca tgtcaccacc tcacagcaga
    13441 cgagtcgcgc ccgttgggtg tgcggggctc acctactcct gctcaaaccg tccggtgaag
    13501 aagcgaagca ccggcggctc atacacccac ttaagcccgc gcacctgatc gcgtcgggca
    13561 tagacctcgg caatggaatc ggctacatag tcaaagtggg cgttggtata cacccggcgc
    13621 ggtatggtca atcgaacaag ctcaagcttg ggctggtagt ccttgccttc cttgtcgcgt
    13681 cctgccgaga taatgcctct ctccatagag cgcactcccg actcttcata gatagcggcc
    13741 gctaaggcct gcgccgggaa cttgggctgc ggaatgtgcg gcaagaacgc cctggcatct
    13801 aggaagacgg cgtggcctcc gaccgggcgc acgatgggta ctccggcctc gatcagcttg
    13861 gcgcccagat agcccacttg ctccacacgg tgctcgatgt aggcgtagtc catggcctcg
    13921 cgcagcccgc gtgcgaaggc ttccatgtcg cgcccggtca tcccgccata gctgggcatg
    13981 ccctcgtaca ctactactct cgacgtagcc tgccggtaca cttcttcctc gttagtggcc
    14041 aggaagccgc ccatgttgac aattccgtcc ttcttgccgc tcatggtgca accgtccgaa
    14101 taggagaaca tctcatgtac aatcgaagcg atagtccgat tctcgtagcc cggttcgcgc
    14161 ttcttgatga agtacgcatt ctccacacac cgagtggcat cgaaaatgac gcggattcca
    14221 tacttcttgg acatctcgta cacggctttg aggttggcca tgctcaccgg ctggcccccg
    14281 gccatgttga cggtgacggc aaccgatatg tacgggatct tgtcggcgcc aaccttcttg
    14341 atcagcgcct cgtacttggc gaggtcgaca tcgcccttga aaggcacgct gtctagctgc
    14401 ggatcgtgtg cctcgtcaat gatgacgtca acgaacgtgc cgccgttcat ctcctggtga
    14461 gcccgggtag tagtaaagta catgttgccc ggcacatacg tcccaggcct tacgaggatc
    14521 tgcgaaatga ggttctctgc tcctcttccc tggtgagtgg ggcagacata cttgaacccg
    14581 aacagctcac gtacagtctc ctccaggtga agccagtttt cgctccctgc gtaggcctcg
    14641 tcacc
//
LOCUS       DAOTAC010000022        14450 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_3521_length_14450_cov_5.001667, whole genome shotgun sequence.
ACCESSION   DAOTAC010000022 DAOTAC010000000
VERSION     DAOTAC010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 14450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14450
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3521_length_14450_cov_5.001667"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="PLM74_01845"
     CDS             <1..314
                     /locus_tag="PLM74_01845"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HON41858.1"
                     /translation="IALVPAIILAGLGVRAPSEQIMGNVILFYMASSYVPTPGASGFA
                     ELGFAELFSLIVPHGLLGLTVALWRLATYYFTLVVGGALVIGQMLYSRTAGPVEDKTR
                     T"
     gene            complement(353..919)
                     /locus_tag="PLM74_01850"
     CDS             complement(353..919)
                     /locus_tag="PLM74_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HON41859.1"
                     /translation="MSSAAKRPEQPRIGLLIKAAMFAAATAALAYVAFPVPFSPVPVT
                     GQSFGAMIAGLLLPPRAAMGSQLAYLLAGAMGLPVYAGGRAGIGVLFGPTGGYLWGLV
                     AGSYITSVMIESGTPHARIPTTLSCIAASVVGGVVVVYALGVVQLALTARLGWLQALV
                     AGALPYLPGDAVKAFVAGIVGARLMRYR"
     gene            2352..3764
                     /locus_tag="PLM74_01855"
     CDS             2352..3764
                     /locus_tag="PLM74_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009250635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HON41860.1"
                     /translation="MASTQHDQGIRAYNQGLMDLLSTAARICISDPAQAGFIARMAGS
                     QRRAARTRERWEEQGAHVPPFLIISVTSECNLHCKGCYARAQHRDPEVGPGLGLEQGL
                     GLEPGVGPEPGSGLEPGPGSAPGMPGTLGAPGVPGESDERGNAGVGTDVTGVAFAAAV
                     STAAAVAAGPGRRDMDDATLRAVLDDARQLGISVVLLAGGEPLTRPGLLDIVSEYPDI
                     LFPIFTNGLLIDDDVAVRLRRQRNTIPVLSLEGNQEITDDRRGSGVWSRVRGAAQALH
                     RARVFFGSSFTVRSDTLDALTDERFIAEMIGLGCRLFFFVEYVPVQPGTEGWELTQDQ
                     RLELARRVEALRSSTPALYVSFPGDEEATGGCLAAGRGFVHISARGDVEPCPFAPYSD
                     SSICGVPLQDALKSPLLEAIRADHDRFGDTSGGCALWRRREQVQALLESRAREQIREQ
                     QGAAASNREQQGANQGANQA"
     gene            3805..3879
                     /locus_tag="PLM74_01860"
     tRNA            3805..3879
                     /locus_tag="PLM74_01860"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3837..3839,aa:Asn,seq:gtt)
     gene            3966..4682
                     /locus_tag="PLM74_01865"
     CDS             3966..4682
                     /locus_tag="PLM74_01865"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HON41861.1"
                     /translation="MSLSGSTILLIEDDPNVRRLLPRYFEKEGAATLVAAGGLEGVDL
                     ADDRRPDLVLLDVNLPDIDGWSVLKRIRRSLGYSVGVIMLTGRSDVPDRLLGLELGAD
                     DYMVKPFEPQEVVARARAVLRRLKTPAPGGSGLRAATGFELDEAGRTLYVDGAAVPLT
                     AKEFDLMVALMQNPGRVVTREVLAVRGWPDGEDVDSHAIDVYMNRIRSKLGPVYSASA
                     ILTVRGVGYKFNPDADGAPR"
     gene            4687..6234
                     /locus_tag="PLM74_01870"
     CDS             4687..6234
                     /locus_tag="PLM74_01870"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HON41862.1"
                     /translation="MIRIGTDTWESLPTLRRHFFARFGVGAVIALAIVLATSLFLADA
                     LAARWAARALLQDLEDGLYDAVRGGMGGAAGMGGSVGMGGAGGTGGAIRTGGAMGMGM
                     RRMMAVGGTLMLAPETLQAYEPVWPDARRIQSSQATYGSGRVPWTREPVVWAARRQED
                     GGEAVQVVWTRLSAVRAAAAGTYSLVAAATIASFAVGACVTDIGVKKVAQAVRAAANT
                     SRQMAEGDFTARMSVGDTRELAELSAAANHLAESLDHTLTQLRSQNEELARLERSQRE
                     FVADASHELRAPLTAMAITLDAAADGLMSEEERAEAWLVLRSEVRRLSRTVTELLDLS
                     RIESGREPLNVSPTDVGKVIEGVYQWYQSLPGALPSFEAEVHSPEGQPLIALADGDAL
                     HRCVVNFVDNAIRATSPTGRISIWAHPLDDGRIGVGVTDTGEGMTPEEVGRAWERFSR
                     SERARAKGSPGSGLGLAIVRALVMAMGGEVGLESDAGKGTTAWIRLRSERPHAQLERH
                     DQYQQGV"
     gene            6324..7169
                     /locus_tag="PLM74_01875"
     CDS             6324..7169
                     /locus_tag="PLM74_01875"
                     /inference="COORDINATES: protein motif:HMM:NF021485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2202 domain-containing protein"
                     /protein_id="HON41863.1"
                     /translation="MNKTSAKTARTGALLAMAVVSALVVGLALGAGGMFTAWAAEASE
                     TQAEEKAGLVYMVEEEKLARDVYAKMFELWGLRIFRNIAGAEQRHIEHVREVLKAQSL
                     SDPTASAKPGEFADARLQNLYDILVKKGSESQEAALTVGAAIEDLDICDLEALKAKTK
                     SDEIASLYNALIAGSENHMRSFTGQLKARGVEYKPEHISPERYSSILAGSNGRNACGP
                     DCDFAGNMGRTGRMGRTSDMGNGFSPGRAGGRGQRHGQRQGQRQGQRNRQARAVGGNC
                     PYAGN"
     gene            complement(7166..8488)
                     /locus_tag="PLM74_01880"
     CDS             complement(7166..8488)
                     /locus_tag="PLM74_01880"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HON41864.1"
                     /translation="MSALMEWALDVFGRFGAAGLFVVAFAESSFFPVPPDVMLLPMCI
                     ARPRAAVWYAAITTASSILGAAFGYLVGARGGRPLLLRFTDEQGLRTVQRLFDKYGGW
                     AVGIAAFTPIPYKVFTIASGIFRVRLTPFFTASIIGRGGRFFLEAVLAVRYGEAFREI
                     VGPRFETITLLVGLVAAVLAIALGAAQRRMRPATETGVVATATRRFARVRLPEPIARF
                     GRRRRDVLAEWLVYLLAGFIFLLIFVEELAELAASGAVPAIRRLDTQIALAMLSIRHP
                     VLTGALTGVSFLSSYPAVFAGLGLAVFLFMSRPGGFRRIHSAALVSCVLGSAGLAILT
                     RLTQTIPGPASASGLHQLLTRSAGAGGLLVSTCFYGMLSVILRRHAAIATSLGPVLAA
                     PILMASSAFAWVYLGLAWPSETAAELIAAGMWLLICAWLMTLVERKQL"
     gene            complement(8514..9410)
                     /locus_tag="PLM74_01885"
     CDS             complement(8514..9410)
                     /locus_tag="PLM74_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41865.1"
                     /translation="MRYKRNKGEWKPPGAPCDDTYGPNHRPDTPDIMEEMEEPAVLGG
                     PIWLQRVLRPKSPLAVLLTLMAIGIAVMAYTEGDPRRVIRLSPPWQPGEISRLEAYDP
                     DGNLLLTWELSVESQGSQVVFITDREAAGFRERALVFADPYTLIPRRTELERESGQGR
                     VDYVAEYGSEDVTIQANLPRGHEEAAMKLPARPFYDDEQLIMIIRALPLYSSFRGTLK
                     SVVTRAGAQDSVILRVRGRESISVPAGHFDTWKVEITGTYQSAWIAVAPPHQLVRYEN
                     RKTGILSELAEYMEGTVAPTEE"
     gene            9623..10255
                     /locus_tag="PLM74_01890"
     CDS             9623..10255
                     /locus_tag="PLM74_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41866.1"
                     /translation="MAQDIAIADLLTQEGFDTLPAFQTAREVLIRKGLTNIRKTNIHV
                     SKLPRVREVLAGELYLVCSDPLCQTLARLECGSRQIVTVSSAGCRICGGKNSRRCLDA
                     ANRLFQQHGICRVVIVGGTGADHSEIARQARGKSVEFRFVDGTRAGRATQLAEAYVRW
                     ADLVIIWTNTPLKHSVSNTYTGNRSRAGRVVYANRTGVAAMISQVARQIA"
     gene            complement(10326..10628)
                     /locus_tag="PLM74_01895"
     CDS             complement(10326..10628)
                     /locus_tag="PLM74_01895"
                     /inference="COORDINATES: protein motif:HMM:NF019199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HON41867.1"
                     /translation="MTQVRCSVDTCQYWGEGQICRADSIWVRDNMGFSPGRSATGSTR
                     MEIADEPGTVIRPGQTTGTHSASAASTSTETCCGTMKPRGGHGGTHAGKHGGRKGT"
     gene            10941..11951
                     /locus_tag="PLM74_01900"
     CDS             10941..11951
                     /locus_tag="PLM74_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016352448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HON41868.1"
                     /translation="MERYATIVGTGIYLPEIEVSNASLAEHFEKVMPGAGMDVVIGKF
                     EAATGIRRRWYAPRDWATSDLAVRAGRQALHAAGLDPRDIDLIILGTDSPDYITPATS
                     VVVQYELGARNAGTYDVGCACASFPTAVAAAAGQLVTNTWMKYVLVIGAYMMSKLAAH
                     DDVTRFYYGDGAGAAVMTVDTKPGFLTASYNADGSYHRNWGIYSGGTFEPATCESVQA
                     GRTQVRFVDKYPPEVNHEGWPKIVRHLAKRGGFQLDDIDMAIFTQVRLASIRVVMEDL
                     GLPLEKTHWIMEDWGYTGSACVPMALDDAVRKGRIHSGDLVVLVGSGVGYNQAGVALR
                     MP"
     gene            11997..13067
                     /locus_tag="PLM74_01905"
     CDS             11997..13067
                     /locus_tag="PLM74_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012543789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HON41869.1"
                     /translation="MAMGNLDGVRSDFVQLEGVRRIKLHVLTAGERTGEPILFIHGNA
                     SSATFWQEAMVCLAPRFQSIAPDLAGYGLTEPLPVDATRGVRDWSDDLHALIESMNLG
                     RVHLVGWSMGGGILLQYLLDNPEMVASLTLVSTVSPYGFSGTRGANGQPCFPDYAGAG
                     AGGVNPEFIVQMKSQNRESGESPMLPRNVLNFTYWKPPFRAAREEDFLTSLLQTAIGD
                     HNYPGDWVPSPNWPGFAPGPDGVLNAFSPKYFDASGIVDLDSKPPILWIRGANDQLVS
                     DTSLWDIAYLGKLGLVPGWPGDDVCPPQPMLAQTRAVLDEYKSRGGCYREVVFADCGH
                     SPHIEKTDEFVKVLTEHIDACQ"
     gene            complement(13120..>14450)
                     /locus_tag="PLM74_01910"
     CDS             complement(13120..>14450)
                     /locus_tag="PLM74_01910"
                     /inference="COORDINATES: protein motif:HMM:TIGR02887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009847 - spore germination [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Ger(x)C family spore germination protein"
                     /protein_id="HON41870.1"
                     /translation="SVRAHVGLTHSKTPVAPPHREARQMRLSAALVALCLALASAGCW
                     DRKEPDDMVFAVALGFDIDDDGDYVAIAQFANPVAVKGGVGPSSQPAGSAPFWTNCAK
                     GRTPFDALANLSPALSREMTVSNIEVLLISERLAKRGIAPIMDLVARDNELRLAVSTA
                     VVDGSLKSLLESQFPEDPIPALSIQRMLNPIHFIDPSAMSPGLLQSITDMLRPGQDLL
                     LPALRFSAGAVSESGEERGPDRESDSSSNSRSAMDSTSPGSPVTHISSAAFHRDKMIG
                     WLDGHESRGANFARTRGQHAPISVQSPAGGLVSIHVAQVESTVTPRYMNEDVSVLIKV
                     KVEGHVQGQTGLEEGQAGVNFANPESLRDLRSCLAQCVREDIEHALAKSREYRSDFIG
                     LGNAVYRRLPRVWKAGIGGRWYELLPYVQVQVEVEATLTSSGLLRESLHY"
BASE COUNT         2857 a         4131 c         4655 g         2807 t
ORIGIN      
        1 cgatcgctct tgtgccggcc atcatactgg cggggttggg ggtacgtgct cccagcgagc
       61 agatcatggg caacgtgatt ctcttctaca tggcgtcctc ctacgtgcct actcctggcg
      121 ccagcggttt cgccgagctg ggattcgcgg agctgttcag cctgatcgtc ccgcatggct
      181 tgctcggcct cacggttgcg ctgtggcgcc tcgcaaccta ctacttcacg ttggtggtcg
      241 ggggcgcgct ggtaattggg cagatgctgt acagccgcac ggcaggtcct gttgaggaca
      301 agacaagaac gtagaatcgc acggcctcac cttacagcct cacctcacag cctcaccgat
      361 atcgcattag tcgagctccc actattcccg cgacgaacgc cttcactgcg tcgcctggaa
      421 gatacgggag cgcgcctgct accagcgctt gcagccaccc gagcctggct gtcaaggcca
      481 gctgaacaac gcccaaagcg tagactacaa caactccgcc gacgaccgat gcagcgatgc
      541 agctgagcgt cgtggggatc cgggcgtgtg gagtgccgga ctctatcatg acggacgtga
      601 tgtacgaacc tgccacaagc ccccagaggt aaccgccggt ggggccgaag agcacgccaa
      661 tgcctgcgcg gcctccggca tatactggca ggcccatggc cccggccagc aggtacgcca
      721 gctgactgcc catggctgcc ctgggcggca gaagcaggcc ggcaatcatc gcgccgaacg
      781 actgccctgt gaccggcacc gggctgaagg ggactggaaa ggccacgtag gctaaagccg
      841 cagtggctgc agcaaacatg gcggccttga tcaggaggcc gatcctgggt tgttcgggtc
      901 gcttcgcagc tgacgacatc tcaccaacat cctcccaata cggtctagtg accgccccgt
      961 ggattcgccg cgtcccgtac aatctcgcca cagtctccat gcgacctccg cacagtctcc
     1021 atacagtctc cacaatctcc cgacaatctc tctacagtcc aatcataacc gcccgcgcgt
     1081 ctttctccaa ctgggttaca ggacttctgc ggttcatctg gcaagggtgg actgcatccg
     1141 caactgccat gggtattgcc ggcttgcccc atcacctgtg ggttttccgt gcggcttctg
     1201 gcgaggccgt actgaatacc gtatggttct gcacacacag gtttcccctt atgcaaccgg
     1261 cggtgggatt cgcgtatacc agacgattgg tgattagcga acgcgcagct aggaccgcca
     1321 taggtacata gcgcctcgac ggtgggccgc cgggtaccgc catcgagagc tctgcgatcc
     1381 ccaaagaatg cccccgattt cgcccgaccg tgccgaaccg tggggttttc catcaggctg
     1441 gcgaaaagac cgcatggttc tccatacagt cgcgcaaagt cctgctcgga agccataggg
     1501 tactgaagaa tgggtaggtc cgattagtct ggtatatcag ccgcagaagt cttggggtta
     1561 cgtgaggggg ccatcctcgc cgaaagggcg tgttagctgg cccaacgacg atcgcaatgg
     1621 gtcaaaacgg cttgggctgg catagggtca gttattggca tcagttttgg agataccctc
     1681 aacttttgag tacatcgatt tcggaggccc ctccaatcgc ctccgaatcg cgaaaattgg
     1741 ggggtatccg acgggtccgg gcagtgaagc aggcggcgag ccgacataat atcccatatc
     1801 atagcgccat ttggcaacgg cgtgtgagtg atggcatgtt atgccagcga ttcgccacta
     1861 aatcggcgcc gtatggcccc cagggctcct tttgcggggc cgattcgccc cccggggcac
     1921 tcggcccaaa tcgatgtact caaaaattgc gtttttcgcc gaaagtatta tcaaaatccg
     1981 acactatctc tgcttggata cccccggggg tatatggtcg agccgtagcg atcggcgttc
     2041 tacgctcggg tctaccctcg ggtctacgct cgggtctacc ctcgggtctg ttcgcgggcg
     2101 ccctcatgct gcaactgctc tcggtgaaat catggcacgg ccgcacgcag agagccaatc
     2161 cggaccagat gggttcagcg gagcgccaaa tgccggatcg gcgcagcgtc gaggagggag
     2221 agatctgcag ctccaggagc gaccatcgct gcacgcaccc agatcaccgg gtgcctcttg
     2281 acacgccgac ccaacactga tattgctgta atgcctatcg tttcatttgg ggccgtgcgg
     2341 aggtggggga tttggcctcg acccaacatg atcagggtat acgcgcatat aaccagggac
     2401 tcatggatct tctgagtact gccgctcgta tctgcatatc tgaccctgct caggccgggt
     2461 tcatcgcccg aatggcgggc tcgcagcgca gggcggctag gacgcgagaa cgctgggaag
     2521 agcagggcgc tcatgtgccg cccttcctca tcatcagcgt cacttcagag tgcaatctgc
     2581 actgcaaggg ctgttatgcc cgggctcagc atcgagatcc ggaggtggga ccggggctgg
     2641 ggttggagca ggggttgggg ctcgaaccgg gggtaggccc ggaaccgggg tcggggctcg
     2701 aaccagggcc cggatctgcg cctgggatgc ccgggacttt aggggcgcct ggggttcccg
     2761 gggaatcgga tgagcggggc aatgcgggag taggtacaga tgtcacaggt gtcgcttttg
     2821 ccgcagccgt ctcaactgcc gcagcggtcg cagccggccc gggaaggcgc gatatggatg
     2881 acgccacgct tagggctgtg ttggacgacg cgagacagct cggcatatcg gtggtgctct
     2941 tggctggtgg cgaaccgctc acaaggcccg gactgctgga catcgtgtcg gaataccctg
     3001 acatactctt ccccatcttt actaacgggt tgctgataga cgacgatgtc gcggtgcggt
     3061 tgcgcaggca aaggaacacg attcccgttc tcagcctcga gggcaaccag gagataactg
     3121 atgaccgtcg gggatccggg gtctggagtc gtgtgcgcgg cgcggctcag gcactccatc
     3181 gtgcaagggt attcttcggt tcatcattca cggtgcgaag cgatactctg gatgcgctca
     3241 ctgacgagcg gttcatcgcg gagatgatag ggctcggctg tcgtctgttc ttcttcgtcg
     3301 agtacgtccc tgtgcagccg ggcacagagg gctgggagtt gacacaggac caaaggcttg
     3361 agctggcgcg tcgcgtggag gctctcaggt cttccacgcc ggcactctac gtgtcattcc
     3421 caggcgacga ggaggccaca ggcggttgcc tcgccgcagg caggggattc gttcacatca
     3481 gcgcgcgggg agatgtggag ccatgcccat tcgctccgta ttcggattcc agcatctgcg
     3541 gagtgcccct ccaggacgcg ctgaaatccc cgctgcttga ggccatccgc gcagaccacg
     3601 accggtttgg cgacacgtcc ggagggtgtg cgctgtggcg gcgacgcgag caggtacagg
     3661 cgcttctgga atcgcgcgcc agggagcaaa tcagggagca gcagggagca gcagcgagca
     3721 acagggagca acagggagcg aatcagggag caaatcaggc ttgacaatgc ccgcaggccc
     3781 tgatatcttg tcgatataag gcgctcctcg atagctcagc ggtagagcaa ccggctgtta
     3841 accggtgggt cgcaggttcg aatcctgctc ggggagccag tattattggt cgtgcgcggg
     3901 ccgctggagg atatccggcg gctcttgtga tattcctacg ttgtttgggg gagggggtct
     3961 tcgacttgag cttgagcgga tcaaccatac tcctaatcga ggacgatccg aacgttcgcc
     4021 gattgctccc aaggtacttt gaaaaagaag gcgccgcaac gctggtcgca gcaggcgggc
     4081 tggagggcgt ggatcttgca gatgacagac ggcccgactt agtgttgctg gatgtcaacc
     4141 tccccgacat cgacggctgg tcggtgctga agcgcatccg ccgtagccta ggttactcgg
     4201 taggcgtcat catgctgact ggtcggagcg atgttccgga taggcttcta gggctggagc
     4261 tgggagctga cgactatatg gtcaagccct tcgaaccaca ggaagtcgtg gccagggcgc
     4321 gcgcggtgct gaggaggctg aagacgcctg cgcctggggg ttcgggcctc agggccgcga
     4381 ccgggttcga gttggacgag gcaggtagaa cgctctacgt ggacggcgcc gctgtgccgc
     4441 ttactgctaa ggagtttgac ctgatggtgg ctctcatgca gaatcctggt cgagtcgtaa
     4501 cccgcgaagt gctggccgtt cgcggctggc cggacggcga ggatgtggat tcgcatgcga
     4561 ttgatgtgta catgaacagg ataaggtcga agttggggcc agtctatagt gcttcggcca
     4621 tactgactgt tcggggcgtt ggatataagt tcaaccccga tgcggacggg gcgccgaggt
     4681 gagcgcatga tcaggatcgg tacggatact tgggaaagcc tcccgactct cagacgacac
     4741 ttcttcgcga gattcggcgt gggcgcggtg atcgccctcg cgattgtcct ggcaacctcg
     4801 cttttcctcg ccgacgcact ggctgctcgg tgggcagccc gcgcgctgct gcaagacttg
     4861 gaggacggac tgtatgatgc ggtccgtggc ggcatgggcg gagccgcggg catgggcgga
     4921 tccgtgggca tgggcggagc agggggaacg ggtggagcca tcaggacggg cggcgccatg
     4981 ggtatgggta tgcgccgcat gatggcagtg ggtggaacct tgatgttggc gcccgagact
     5041 ctccaggcat acgaacctgt ctggccagac gcgcgacgca ttcagagcag ccaggcgacg
     5101 tatgggtcag gacgagtgcc ctggacccgc gagccggttg tgtgggcggc cagaaggcag
     5161 gaggatgggg gcgaagcggt ccaggtggtc tggacacgcc tatcggctgt tcgcgctgcc
     5221 gccgccggga cgtacagtct ggttgcagca gctacaatag cgtcgtttgc tgtcggcgca
     5281 tgtgtgaccg atatcggagt gaagaaagtc gctcaagcag tgcgtgcggc tgcgaacacg
     5341 agccgacaaa tggcggaggg cgacttcacg gcccgcatga gcgtgggcga cacccgggaa
     5401 ctcgctgagc tgtcggctgc tgcaaaccac ctggcggaat cgctcgacca cacattgacg
     5461 cagctgaggt cgcagaacga ggaactggcc aggctcgaac gttcgcagcg ggaattcgta
     5521 gctgacgcgt cacacgagct gcgcgcgccg cttacggcca tggcgataac cctcgacgct
     5581 gctgctgacg gactgatgag cgaggaagag agggcagagg cctggcttgt gttgcgctcc
     5641 gaggtccgca ggctctcgcg aaccgtgact gaactgctgg atctttcgcg aatagagtcc
     5701 ggtcgagagc cgctcaacgt gagcccgacc gatgtaggta aggtcattga gggtgtttac
     5761 cagtggtatc aatcgctgcc gggagcactt ccgtctttcg aagccgaagt acattcgccg
     5821 gaaggacaac ccctgattgc cctggccgac ggcgatgctc ttcatagatg cgtggtgaac
     5881 ttcgtggaca acgcaatccg agccacttcg cccacagggc gcatctctat ctgggctcat
     5941 ccgctcgatg atggccggat tggggtcgga gtcaccgaca caggcgaggg catgaccccg
     6001 gaagaggtcg gccgtgcctg ggagcgattc tcccgctcgg agcgggcccg ggccaagggc
     6061 agccccgggt ccggcctggg actggccatc gttcgcgcgc tggtcatggc gatgggcggc
     6121 gaggtgggcc tcgaatcgga cgcgggcaag gggacgaccg cctggattcg tctgagatct
     6181 gaaaggcccc atgcgcagct tgagcggcat gatcaatatc aacaaggtgt ttgatgcttc
     6241 attgaggtga tgttgaggtt gcgcctttac catctgaagt gagaacagtt cataggacat
     6301 ttagatggag gcgaaacatc aacatgaaca agacttccgc gaagaccgcg cgaacaggag
     6361 ccttgcttgc catggccgtc gtttcggcac tcgtggtcgg actcgcactg ggagcgggcg
     6421 gcatgttcac ggcatgggca gctgaggcgt ctgagactca ggcagaggag aaggcgggcc
     6481 tcgtatacat ggtggaagag gagaaactag cgcgcgacgt gtatgccaag atgttcgagc
     6541 tctggggcct aaggatattc cggaacattg ccggcgcgga acagcggcac atcgagcatg
     6601 ttcgcgaagt gcttaaggcg cagagcctga gcgatcccac ggcgtcggcc aagcccggcg
     6661 agttcgccga cgcgcgcctg cagaacctgt atgatatcct cgtcaagaag ggctcagagt
     6721 cgcaggaggc ggcgcttaca gtgggggcgg caatcgagga cctggacatc tgcgacctcg
     6781 aggccctgaa ggccaagacg aaaagcgatg agattgcgtc tctttacaat gctctgattg
     6841 ccggttccga aaaccatatg aggtcgttca ccggacagct taaggcgcgg ggagtggagt
     6901 acaaaccgga gcacatctcg cctgagagat actcatcgat tcttgcgggt tccaacgggc
     6961 gcaacgcgtg cggcccagat tgcgacttcg cgggcaacat gggcaggacg ggcagaatgg
     7021 gcaggacgag tgacatgggc aacggcttca gcccaggccg ggccggaggt cgcggtcaga
     7081 ggcatggcca gaggcagggt cagaggcagg gccagaggaa ccgtcaagcc cgcgcagtgg
     7141 gcggcaattg cccgtatgca ggcaactaga gttgttttct ctcaactaga gtcatgagcc
     7201 acgcacatat cagcaaccac atgcctgcgg cgataagctc cgctgccgtc tccgatggcc
     7261 atgccagccc aaggtagacc catgcgaatg cgctggaggc catcaggatg ggcgctgcca
     7321 agaccgggcc gagcgaggtt gcgatggcgg catgccgcct caagatgact gacagcatgc
     7381 catagaagca ggttgatacc agcagccctc ctgccccagc gcttcttgtg agtagctggt
     7441 gcaggcctga cgccgacgca gggccaggga tggtctgggt cagcctggtg agaatggcca
     7501 gcccggcgga tcctagcaca catgatacca gagccgcact gtgaatgcgc ctgaaaccgc
     7561 cagggcgcga catgaacagg aataccgcaa gtcctagtcc ggcaaacact gctggatacg
     7621 agctcaagaa ggacacgcca gtgagcgctc cggtaaggac aggatgacgg attgacagca
     7681 tggccagagc gatctgcgtg tctaggcggc ggatggcagg cacggctccg gaggcggcaa
     7741 gctcggcgag ttcttccacg aatatcagca ggaagatgaa gcctgccagg agatatacga
     7801 gccactctgc gaggacgtct cttcggcgcc ggccgaaccg cgctatgggc tccggtagcc
     7861 ttaccctggc gaacctgcga gtggcggtgg cgactacgcc tgtttcggtc gcggggcgca
     7921 ttcgcctctg tgctgcgccg agggcaatcg cgaggacggc tgccactaac cccaccagaa
     7981 gagtgattgt ttcgaacctg ggcccgacta tctccctgaa agcctcgcca tacctcactg
     8041 ccaggactgc ttcgaggaag aaacggccac cccgcccgat gatcgaagct gtgaagaagg
     8101 gggtgagccg cacgcggaag atccccgagg cgatggtgaa caccttatat gggatggggg
     8161 taaaggcagc tatgcccaca gcccagccgc cgtacttgtc aaacagcctc tgaacggtgc
     8221 gcaggccttg ctcgtcggtg aacctgagca acagagggcg gccgccgcga gctcccacga
     8281 gatacccgaa tgccgcgccg agtatggatg aggccgtagt gatagccgca taccagaccg
     8341 ctgcccgagg acgcgctatg cacataggca acagcatcac gtcgggcgga acagggaaga
     8401 aacttgattc ggcgaacgcc acgacgaaca aaccagcggc gccaaaacgt ccgaagacat
     8461 ccagggccca ttccatcaat gcactcatag cgtaccagcc gttccttcat aggtcattcc
     8521 tcggtcgggg ctactgtccc ttccatgtac tccgccagtt cgctgagaat gcctgtcttc
     8581 cggttttcgt acctgaccag ctggtgtggg ggcgcgaccg caatccacgc ggactggtat
     8641 gttcctgtga tctcaacttt ccaggtatcg aaatgccctg ccgggaccga gatactttct
     8701 ctgcctcgaa ctcggaggat cacgctgtct tgcgccccgg ctcgcgtaac gacgctcttg
     8761 agagtgcccc ggaaactgct gtagaggggg agggctcgaa tgatcataat gagttgctca
     8821 tcatcataga acgggcgcgc gggcagtttc atcgcagctt cctcatgtcc acggggcaag
     8881 ttggcttgaa tcgtcacgtc ttccgatccg tactctgcca cgtaatcgac acgcccctgg
     8941 cccgactcac gctcaagttc ggtccttcgg ggaatgagcg tgtatggatc cgcaaagacc
     9001 agggcgcgtt cgcgaaagcc cgctgcctct cgatccgtta tgaagactac ctgcgaacct
     9061 tgtgactcca cgctgagctc ccatgtgagc aacaggtttc cgtcgggatc gtacgcctcg
     9121 agacggctga tctcccctgg ttgccacggc gggctgagcc gaatcacgcg cctcgggtcg
     9181 ccctctgtat aggccattac ggctatgcca atcgccatca gagtcagcag gacggccagc
     9241 ggtgacttgg gcctcaggac ccgctgaagc catatcggcc cgcccaggac agcaggctcc
     9301 tccatctcct ccatgatgtc gggggtgtct ggacggtgat tcgggccgta ggtatcgtcg
     9361 catggtgccc ctggaggttt ccactcgccc ttattcctct tgtagcgcat caagcaatgc
     9421 tcctctctcc ggagacgaaa gacgcggcag cggcaggtcg catcactcat tggttcgcca
     9481 tggcatgcat cggaaccttc acatgggcaa ttcacatttt cagtatgccc gagacgcatc
     9541 ccggcggcac gggttgtgac aaaacgacgc cggggccctg tgatatgctg gtcacaccaa
     9601 aacgagatgg gaggctgacc agatggctca ggatatcgcc atagcggatc tgttgacgca
     9661 agaaggattc gacactctgc ctgcctttca gacggctcgt gaggtgctga taagaaaagg
     9721 actgacaaat atacggaaga caaacataca cgtttccaag ctgcctcgtg tgcgagaggt
     9781 gctcgcggga gagttgtacc tcgtatgtag cgacccgctg tgccagacct tggccaggct
     9841 cgaatgcggc tcacggcaaa tcgtaaccgt ttcgtccgca ggctgtcgga tctgcggagg
     9901 caagaactcg cgaagatgcc tggatgcagc aaatcgtctg tttcagcagc atgggatatg
     9961 ccgggtggtc atagttggag gaaccggcgc agatcattct gagatagcga ggcaggcgcg
    10021 aggcaagagc gtggagttca ggttcgtcga cgggaccagg gcgggcaggg cgactcagct
    10081 agcagaggcg tatgtcaggt gggcagatct ggtgatcatc tggaccaaca cgccgctgaa
    10141 gcattcagtc agcaatacat acaccggcaa tcgaagcagg gccggaagag tcgtatacgc
    10201 taaccgcaca ggagtggcgg cgatgataag ccaagttgcc aggcagatag cctgacaact
    10261 tggttaatca gccaatggcg cgccggggcc cccgcttggc cgggagagcc ccggtattct
    10321 tgtggttatg ttcccttcct gccgccgtgt ttgcctgcgt gtgtgccgcc gtgccctccg
    10381 cggggtttca tcgttccgca gcaggtttcc gtggatgtgc tggcagcgga ggctgagtgc
    10441 gttccggtgg tctggccggg ccggatcacc gttccaggct cgtcggcgat ctccatccgc
    10501 gtggaacctg tggcgctgcg accggggctg aaccccatgt tatcgcgcac ccagatcgaa
    10561 tcagcccgac atatctgtcc ttcaccccag tactggcatg tgtcgaccga gcatctcact
    10621 tgggtcatac cgtcacctcc cggtttcatc ggtcgatggt agtctgctca aatggaggac
    10681 ttttctaccg tactcaccat gggggtggcc tgcggcgggg cgacttcctt cgattcccat
    10741 acaggtgagg cgcatataca tcgccggcca gccgactgca accacctggg ccgtgcgaag
    10801 gatagcgcct ggcgcatgca gaatcatcag agtattgcgc gcgtgtgcgg taccggtggg
    10861 ttatccggca gcaacaggac tggcaaacag gactggcaaa cggaactgga caacaggact
    10921 atgcaatggg ggaatatgat gtggaacggt atgcgaccat tgtgggcacc ggcatttatc
    10981 ttcccgagat tgaggtgtcg aatgcaagtc ttgcagagca ctttgaaaag gtaatgcccg
    11041 gcgcgggaat ggacgtggtg atcggcaagt ttgaggccgc tacaggcata aggcgaagat
    11101 ggtatgcgcc gcgcgattgg gctacgtccg acctcgcggt tcgcgcaggc cgacaagcgc
    11161 tacacgcagc gggcctggat cctcgcgaca tcgacctcat catcctgggc accgacagcc
    11221 cagactacat cacccctgct acatcagttg tagtccagta tgagctgggc gcgcgcaacg
    11281 caggtacgta tgacgtgggc tgtgcgtgtg cgtccttccc tactgcggtc gctgctgccg
    11341 ccgggcagct agttacgaac acctggatga agtacgtcct ggtgatcggc gcctacatga
    11401 tgagcaaact ggccgcgcac gacgatgtca cgcggttcta ctacggggac ggggcagggg
    11461 cggcggtaat gactgtcgat accaaacctg ggtttctgac tgcctcctac aacgccgacg
    11521 gctcctacca cagaaactgg ggcatctatt ccggcggcac ttttgagcct gctacatgcg
    11581 agtctgttca ggccggacgg acgcaggtga ggtttgtcga taagtacccg cctgaggtga
    11641 atcacgaagg atggcccaag atcgtgcgcc acttggccaa gcgcggcggt tttcagctcg
    11701 acgatatcga tatggccata tttacgcagg tccgcctggc ttccatcagg gtggtcatgg
    11761 aagaccttgg ccttcctctt gagaagactc actggatcat ggaggactgg ggctataccg
    11821 gttctgcctg cgttcccatg gcattggacg acgcggttcg caagggcagg atacactcgg
    11881 gcgacctggt tgtgctcgta ggatcgggcg tgggctataa ccaggcaggc gtggcgctgc
    11941 ggatgccgta gctcaggatg tcgtagcccg cttcaagatc gatcggaggt gcactcatgg
    12001 caatgggcaa cctggatggt gttcgttcgg acttcgttca gctagaaggc gtgaggcgca
    12061 tcaagcttca cgtgctgacc gccggggagc gcaccggcga acctatactg ttcatacacg
    12121 gcaatgcctc atcggcaacg ttctggcagg aggctatggt ctgcctggcg ccgcggttcc
    12181 aatccatagc cccagacctc gctggctatg gcctcaccga gccgctgcct gtggatgcta
    12241 cgcgcggggt gcgtgattgg tctgatgatc ttcatgcact gatcgagtcg atgaacctgg
    12301 gacgtgttca tctcgtgggc tggtcgatgg ggggcggcat cttgcttcag tatctccttg
    12361 ataatccgga gatggtggca agcctaacac tggtctctac ggtttcgccc tacgggtttt
    12421 ccggcactcg tggagcaaac ggccagccgt gctttccaga ttacgccggg gcgggcgcgg
    12481 gtggggtcaa ccccgagttc atagtgcaga tgaagtcgca gaaccgggag tcgggcgaaa
    12541 gccccatgct gccgcggaac gttctcaact tcacctactg gaaacctccg ttccgggcgg
    12601 cgcgggagga ggacttcctc acatccttgc ttcagacagc cataggagac cacaactacc
    12661 ccggggactg ggtcccatct cccaattggc ccgggttcgc ccctgggccg gatggtgtgc
    12721 tcaacgcttt ttcaccgaag tacttcgacg ccagcggaat tgtagatctc gactcaaagc
    12781 cgcccatact gtggattcgc ggcgcaaatg atcagctcgt gtcggacacg tctctgtggg
    12841 atatcgccta cctcgggaag ctgggattag tgcctggctg gccaggcgat gatgtgtgcc
    12901 cgccgcagcc tatgctggcg cagacgcgag ctgtgttgga cgagtacaag tcaagaggcg
    12961 gttgctatcg ggaagtggtc ttcgcagact gcggccattc gccgcacata gagaagacag
    13021 atgagttcgt gaaggttctg acggagcaca tcgacgcctg ccagtaatgc gtgccggcga
    13081 cgtgcggcca tgggcggtgg tgcgtagcca ggagtggtgt cagtagtgga gactctcgcg
    13141 cagcaggccg gagctggtca atgtggcttc cacttcgacc tggacctgca cgtagggcag
    13201 caattcgtac cagcggcctc cgatgcctgc cttccagacc cggggcaggc gccggtagac
    13261 agcgttgccg aggccgatga agtccgatct gtactcccgg gattttgcga gtgcgtgctc
    13321 gatgtcctcc cgaacgcatt gggcaaggca tgaccgcaga tctcggaggg attccgggtt
    13381 agcgaaattg acgccggcct gtccttcctc caggccggtc tgaccctgaa catgtccttc
    13441 taccttgact ttgatgagca ctgacacatc ttcgttcatg tagcgcgggg tgacggtgga
    13501 ctcaacttgg gcgacgtgta tgcttacaag gcctccagcg ggtgactgaa ccgagatcgg
    13561 ggcgtgctga cctctggtcc gggcgaagtt tgcgccacgc gattcgtggc cgtcgagcca
    13621 tccgatcatc ttgtcgcggt ggaaggccgc cgagcttatg tgcgtcactg gagacccagg
    13681 ggacgtagaa tccatggcag atctggagtt tgaggaagaa tccgattccc ggtcgggacc
    13741 acgctcttcg ccggattcgg aaactgcgcc ggcgctgaac cggagtgcgg gcagcaacag
    13801 gtcctggccg ggccgcagca tgtcggtgat actctggagg aggccgggcg acatcgcgga
    13861 aggatcgatg aagtggatag ggttgagcat tcgctgaatg ctcagagcag gtatgggatc
    13921 ctcggggaac tgcgactcca gtaaggactt tagacttccg tcgacgacag cggtgctgac
    13981 ggcaaggcgc agttcgttgt cgcgtgctac caagtccatg atgggggcta tgccgcgctt
    14041 tgctagcctt tcggagatga gcagaacctc tatgttcgac acggtcatct cccggctgag
    14101 cgccggtgat aggttagcca gggcatcgaa aggagtgcgg cctttggcgc agttggtcca
    14161 gaagggcgct gatccggccg gttgcgaact gggcccgacg ccgcccttga cagccacagg
    14221 gttggcgaac tgcgcgattg ccacataatc tccatcatcg tctatgtcga agccgagcgc
    14281 cacggcgaac accatgtcgt cgggctcctt ccgatcccag cagcccgcgg aggcaagcgc
    14341 aaggcagagt gcgactagcg cggcgctcag tctcatctgt cttgcctccc tgtgcggcgg
    14401 cgcaacaggt gtcttgctat gtgtgaggcc cacatgagcg cgtacggaac
//
LOCUS       DAOTAC010000023        13986 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_3681_length_13986_cov_4.897351, whole genome shotgun sequence.
ACCESSION   DAOTAC010000023 DAOTAC010000000
VERSION     DAOTAC010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 13986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13986
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3681_length_13986_cov_4.897351"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="PLM74_01915"
     CDS             complement(<1..413)
                     /locus_tag="PLM74_01915"
                     /inference="COORDINATES: protein motif:HMM:NF012879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HON41871.1"
                     /translation="MLEMELDKSELLREEVAHTRLDCGLEVATIQKRGFAKKYAVLAT
                     RYGSCDTEFRIAGTANPVSTPEGIAHFLEHKLFEEENGSIDDIFADLGAYSNAFTNYT
                     MTGYLFSCVDNFWPAFDTLLDFVMRPHFTDENVEK"
     gene            complement(406..1701)
                     /locus_tag="PLM74_01920"
     CDS             complement(406..1701)
                     /locus_tag="PLM74_01920"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HON41872.1"
                     /translation="MEEVFQVERLPIGADLHTMRVTKFKTNLLAAVLQSDLSVDGATR
                     NALLPQVLRRGTRRWPDTADFMRNLDDLYGTSVTADVSKKGERHLLMFAVGVVNDAYL
                     DGGDAMLRKGFSILRELMVEPAAEGTGLRADYVAQEKTNLRRRIESILDDKQAYAFRR
                     CNEEMCRTERYAVSKLGRVEDIDAITPDSLADWHAQAVQEYPMDIFAIGDIEHEQAAE
                     LATEAFQGLARHPRSVGPASTQGPVPPSEPRRIVERMPVSQGHLVMGYRTGVTYASSD
                     YPALLFANGVFGGYTHSKLFQNVRERASLAYFASSGIEQTKGLMWVNAGIDFDAFDTA
                     VGIIEEQLEDVRSGAITGSEMENTRLGLIRDIEMMRDQPSSLMDMALAGIINSCPVTP
                     EHLKQSISQVSVDDVVRVARQIRLDTVYFLRNEEGRHHA"
     gene            complement(1896..3629)
                     /locus_tag="PLM74_01925"
     CDS             complement(1896..3629)
                     /locus_tag="PLM74_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HON41873.1"
                     /translation="MGDVTLTIDGRQVTVPAGTSVLEAATQAGAKIPTLCYLEGLNEI
                     GSCRLCVVEIEGAKTLAASCVTPAAQGMVVHTNTRQVREARRAVLELIISNHPFECLT
                     CVRSGSCELQALAESLGIREIRFEGAKAHHAIDDSTPSIVRDPDKCILCRRCVAVCEQ
                     VQGVSAINVSGRGFDSVVSPAGQLPLNDAACTLCGQCILVCPTGALSEVDSTRKVWEA
                     LGDPKKHVVVQTAPAIRVALGEEFGLAPGSIVTGQLVAGLRALGFDRVFDTNFTADLT
                     ILEEGNELLHRLTTGGTIPMITSCSPGWIKFIEHFYPDMLGNLSTCKSPQQMFGALAK
                     TYYAEKAGVDPADMFVVSIMPCTAKKFECERPEMRSSGYQDVDAVLTTRELARMLRES
                     GIDPTALEPASYDAPLGIGTGAAVIFGATGGVMEAALRTVYEVVMKKELPSLDFREVR
                     GFEGIKEAEVVLGDTTVRVAVAHTLGNARKLLDRVRSGEASYHFIEIMACPGGCIGGG
                     GQPIGTNTERRLERIDAIYQADRDLPLRKSHDNPEVKQLYEEFLGEPLGHKSHELLHT
                     HYTPRGIWPKR"
     gene            complement(3656..6703)
                     /gene="nuoF"
                     /locus_tag="PLM74_01930"
     CDS             complement(3656..6703)
                     /gene="nuoF"
                     /locus_tag="PLM74_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HON41874.1"
                     /translation="MDLVRAHVLVCGGAACVSSGCKAVREALEAEIAARGIEREIKVV
                     VTGCMGPCDLGPIMVVYPEGVFYKKLTPDDAREIVDEHLVKGRIVKRLLYTEPGTAEV
                     VETQEAMTFFSRQERIALRNTGVIDPLNIDEYIARDGYAALGKALTEMKPEEIVDTLK
                     RAGLRGRGGAGFPTGIKWDLTRKAEGSPKYVVCNADEGDPGAFMDRSILEGDPHTLLE
                     AMAIAGYTVGANQGYIYVRAEYPLAVAHLEHAIRQAREYGLLGANIFGTGFDFDIDLR
                     VGAGAFVCGEETALLASVESRRGEPRPRPPYPAASGLWGQPTLINNVETYANIAPIIL
                     RGAEWFSSIGTEKSKGTKVFALAGKINNTGLVEVPMGTALGDIIFDLGGGIPGGKKFK
                     AAQTGGPSGGCIPAQFLNTLIDYESLKELGTMMGSGGLIVMDEDTCMVDLAKFFMQFI
                     QDESCGKCAPCRIGTKRMLEILDRITKGQGREGDIELLIELGEHIRTTAMCGLGQSAP
                     NPVMSTIRYFRDEYEAHIRNKKCPASSCAALFNSPCQNSCPAGVDVPRYIALIKQRRF
                     DEAVDLIRATNPFPAICGRVCDHPCESKCRRGTFDEPVAIKSLKRFAADWEIKNKHQY
                     RADFAPPSGKCVAIVGAGPAGLSAAHYLALFGHKVTVFEALPVAGGMLTVGIPEYRLP
                     RDIMEFEIEAIRKAGVDIRLNSRVGVDVTLDQLKTEYDAVFVSVGAHNSAAMGVPGED
                     LEGVLPAVEFLRDAALGRLVKVGRNVAVVGGGNAAIDAARTALRLGAKEVHIVYRRLR
                     SDMPADPAEIEAAIEEGIQVHYLTAPTRLIGADGKVTQMECVRMRLGDFDKSGRRRPV
                     PVEASRTIVDVDTVIAAIGQSVDTSFAGKPADGQEALLMTKSGLVITDPDTMQTNVPG
                     VFAGGDCVTGPATVIAAIGAGRQVALEIDKFVGGAAWQHADVDVERVLVGTPIEEQCK
                     RCVMPEMPVPERARNFKEVELGLTEDMAVAEAARCFQCDVREP"
     gene            complement(6746..7249)
                     /locus_tag="PLM74_01935"
     CDS             complement(6746..7249)
                     /locus_tag="PLM74_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013432578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HON41875.1"
                     /translation="MGCCETKCAGDITDITPEQWAKVDEIIAGYQGQRGVLIPCLHEI
                     QQIVGYLPIEVQERVAAGLDIPVADVYSVITFYALFTTKPKGKHKVGVCLGTACYVRG
                     GDKILEKVEGALGIKAGDTTPDRLFSIQVMRCIGACGLGPVMTVDDDIYARIKPDRVP
                     EVLKKYS"
     gene            complement(7524..9695)
                     /locus_tag="PLM74_01940"
     CDS             complement(7524..9695)
                     /locus_tag="PLM74_01940"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HON41876.1"
                     /translation="MDLIETRDDLCTRCYACVRACPVEAMHAAEGAVRADGETCIGCG
                     ICLEECSHGAIALADHTQLALDLLAQGDTAAVISPGFPGAFPDDDPLRVIGALRALGF
                     AEVWEAAAGAEMLASTYRVALESALGRTVIASTCPAAVRLIEAHRPDLVQNLAPAVSP
                     EIAASRAARASGRPRVVCITPCAAFRLECGDAGRGRVSPAARRIEGGADVVLTFSELS
                     ELFDKSGLRVDQCPPSAPDVAAEGPGRVLPLPAGLLHSVEASDGAFRRGAVNAAGFRE
                     CMEVLDAMSSGGITAPVVELSICRGCVDGPGYPENLGAWRRRQAVLAYSAAMESAGGT
                     SVLERLACSGAGRIDLSRTFFSSLSSEAAPAHCRERAALGTRSGDPDASQTGKMERGR
                     YARSASHSFAADSEPVDMVAYSPAMESVIALMQKVSSVSSTVLITGASGVGKEVVARY
                     IHRTGNRSAEPFVKINCAAIPETLLESELFGYEEGAFTGARRSGKPGLIELASGGTLF
                     LDEITEMPMALQAKLLQVLQEKCLVRVGGTKQIRVDARVIAATNRDIERAVKEGRFRN
                     DLYYRLNVIPIRVPSLVERAGDIAPLTYHFLEKWGARYGRPRRISREAREALERYGWP
                     GNVRELENVLEFLVVTVDAPVIGIEHIPTHIKSAGAKNTAEVCVSGIMPLRKAVEEVE
                     RQLIERASATCPSTYAIARALGVNQSTIVRKIQRFANGRSG"
     gene            11042..11950
                     /locus_tag="PLM74_01945"
     CDS             11042..11950
                     /locus_tag="PLM74_01945"
                     /EC_number="1.4.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005838684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047850 - diaminopimelate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate dehydrogenase"
                     /protein_id="HON41877.1"
                     /translation="MQRTRVAVVGLGNVGRGAAEALAVSPDFELAGVVRRRAEPGARV
                     VGPNWEAPAVTRMDQLEEVRAALMCGPTRELEPTEHALLRSGISVVDTYDIHGEPLWR
                     HKQSLAASAAEGQATAVIAAGWDPGIDSVIRALLEIIAPTGITYTNFGPGMSMGHSVA
                     VRAIPGVADAVSVTLPAGYGRHRRAVYVELDGSRPFADVASDIRCDPYFVRDETDVFE
                     VHSASQAADPGHGVVIDRKGIAGRTGNQLLRWEMRITNPAVTGQIMVGALRAGLKKAP
                     GAYTMIELAAADMLPGTLEDFVKRMV"
     gene            complement(11963..>13986)
                     /locus_tag="PLM74_01950"
     CDS             complement(11963..>13986)
                     /locus_tag="PLM74_01950"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HON41878.1"
                     /translation="HARLAGIAIILLRSVIVAACIMFGLHKLAIRPGLITTVIPAAAL
                     SVAIWMLGSRFNSRVVSVVASAFDMGFISVCVHFGGGLSSEAYALYFVALSIIALQES
                     AISSSLGGIVCAALYGAATFPWSQEPAIPGWTLPYRCGVIMFAGGAIGLMSQASKECA
                     ARLASARDKLDRRAHWDQVLARVTRKINSGIGLRATLELILDGGLEVLGGDFGMVALK
                     DRRGRLVASACRNSPPGLLGRVFRKDEGLVNVAVAQRQTVRVSDYASFSGAIPEVVAE
                     GLSAVAATPLLSGPDCIGALTFGNRRSGAAYTPEDCEFLEMLGKQLSVVVGNAHLLDE
                     VQRRADYLSTLNEISMSLTSVLERDALFEKIYREVCRIIPVDAFFVAIFHAGSREIEM
                     AFLMDQGVRSPSTRFGMESGPTAKVITSGQPILFNRQEDEMMDGERRLGHNVDEVTAS
                     VIIVPMRVGAHVVGAVSAQSYTMNAYGEEERDLLMTIASSAAIALENARLYQSARELS
                     LTDDLTGLGNYRYFCDLLDKEIERAIRHSTPLTLVMLDSDHLKYINDKYGHAIGDTHL
                     IHLADTMRAVARKSDILARYAGDEFMIILPNTAGADGLVLAERLRAEMERTPLMVDGT
                     PVTATISLGVASMPQSGGSVDALVRAADAAMYEAKRQGKNRVMEARCPA"
BASE COUNT         2831 a         4460 c         3834 g         2861 t
ORIGIN      
        1 tccttctcca cgttctcgtc tgtgaaatgc ggcctcatca cgaaatcaag gagagtatcg
       61 aaggccggcc agaagttgtc gacacacgag aacagatagc ccgtcatcgt gtagttggta
      121 aaggcattag agtaggcgcc aaggtcggcg aagatgtcgt cgatggaacc gttctcctcc
      181 tcgaacaact tgtgttcgag gaaatgggct atgccttctg gggtggatac aggattggcg
      241 gtcccggcga tgcggaactc ggtatcgcaa gacccatatc tggttgcgag cacggcgtat
      301 ttcttagcga atccgcgctt ctgtatagtt gccacctcga gtccgcaatc gagccgggtg
      361 tgggcaacct cctcgcgcag cagctcagac ttgtccagct ccatctcaag catgatgacg
      421 accctcctcg ttccgcagga agtagactgt gtccagccgg atttggcgtg ctaccctcac
      481 gacgtcatcc actgagacct gggatatgct ctgcttgagg tgttcgggag tgaccgggca
      541 gctattgatg atgcctgcga gggccatgtc catcaaggaa gacggctgat cccgcatcat
      601 ctctatgtct ctaatcaggc ctagccgcgt gttttccatc tcgcttccgg tgatagcgcc
      661 gcttctcaca tcctccagtt gctcctcgat gatgccgacc gcggtgtcga atgcatcgaa
      721 atcgatgcca gcatttaccc acatcaatcc cttggtttgc tcaatccctg acgaagcaaa
      781 ataggctagg ctagcgcgtt cccgaacatt ctggaacagc ttggaatggg tgtaccctcc
      841 gaacaccccg tttgcaaaga gcagcgctgg gtaatcggaa gacgcatatg tcactcccgt
      901 tcgatatccc ataactaagt gcccctggct gacgggcatc cgctccacaa tgcgtctggg
      961 ttcggaaggc ggcacagggc cctgggtgga tgcagggccc accgagcggg gatgtctcgc
     1021 taagccctga aaggcctccg tcgccaattc cgctgcttgt tcatgttcga tatcacctat
     1081 ggcgaagatg tccattgggt attcctgaac cgcctgggca tgccagtctg ccaaagagtc
     1141 aggagtgatc gcgtctatgt cctccactct acctagtttg ctcacagcat agcgctcggt
     1201 ccgacacatc tcctcgttgc agcgccgaaa cgcgtaagcc tgcttgtcgt cgagtatgct
     1261 ctcaattctg cgccgcaggt tagtcttctc ctgggcaacg tagtctgcgc gcaacccggt
     1321 cccttccgcc gcaggttcca ccatcagctc gcggaggatg ctgaaccctt tccggagcat
     1381 ggcgtcaccg ccgtccagat acgcatcgtt gactactccc acagcgaaca tgagcagatg
     1441 tctttcgccc ttctttgaca cgtccgcagt cacagaagtg ccgtacagat cgtccaagtt
     1501 gcgcatgaag tcggctgtgt ccggccatct cctggtgcct cgccggagaa cctgcgggag
     1561 caaggcgtta cgcgtagccc cgtccaccga aaggtcagat tgcagcaccg ctgcaagaag
     1621 gttggtcttg aacttcgtca cacgcattgt gtgtaggtca gcccctatcg gcagacgctc
     1681 aacctggaaa acctcctcca aagcgaaccc cccaagcact ccacgaaatc gcatacctta
     1741 gtatggccca ttattgccgt gcagttcaag tgcgtcctca atccgctgca tcctcaatcc
     1801 gtcaatccga gcgactacgc catacgcgat gcgaatgggg ggcggttcgc ccgccccccg
     1861 attgacctga tcatgagatc atgtacgatt aacggctaac gcttaggcca gatccctctg
     1921 ggtgtgtagt gcgtatgcag cagttcgtgt gacttatggc ccaagggttc gccgaggaac
     1981 tcttcgtaga gctgctttac ctcggggttg tcatgggact tcctcagagg aagatcgcga
     2041 tcggcctgat atatggcgtc tatcctctcc aggcgtctct cggtattggt gcctatgggc
     2101 tgtccacctc cgcctatgca tccgccgggg cacgccatta tctcgatgaa gtggtaggag
     2161 gcctcgccgg atcgcaccct atcaaggagt ttgcgggcgt ttccgagtgt atgagccaca
     2221 gccacgcgta ccgtcgtgtc gcccagcaca acctcggctt ccttgatccc ttcaaagcct
     2281 ctgacctcgc ggaagtcgag tgaaggcagt tccttcttca tgactacctc gtatacggtc
     2341 ctgagagccg cttccatgac gccgcctgtc gctccgaaga tcactgcagc gccagtgccg
     2401 atccccagcg gggcatcgta actagcaggc tccagggccg tcggatcgat gcccgactcg
     2461 cgcagcatgc gcgcgagctc tctggtggtc agaaccgcgt ctacatcctg atagcccgaa
     2521 gaacgcatct ccggtcgctc gcactcgaac ttctttgccg tgcacggcat gatcgacacc
     2581 acgaacatgt cggcagggtc aaccccggcc ttctcggcat agtacgtttt ggcaagcgcc
     2641 ccgaacatct gttgaggcga cttgcacgtc gacaggttgc ccagcatgtc ggggtagaaa
     2701 tgctcaatga acttgatcca gcccgggctg cacgaggtta tcatgggaat agtgccgcct
     2761 gtcgtgagcc tatggagcag ttcgttgcct tcttccagga ttgttaggtc ggccgtgaag
     2821 ttggtgtcga aaacgcgatc gaagcccaga gccctaaggc ccgcgaccaa ctgcccagtc
     2881 actatcgaac ccggcgccaa gccaaactcc tcgcccaggg caacacgaat ggccggcgcg
     2941 gtctgtacga ctacatgttt cttgggatcg cccagcgcct cccagacctt gcgtgtcgaa
     3001 tctacctcgc tcagggctcc ggtggggcac actagtatgc actggccgca cagggtgcaa
     3061 gctgcgtcgt tcagaggaag ctgccctgcc ggactcacca ctgagtcgaa cccgcggcca
     3121 gacacgttga tcgcagacac accctgaacc tgctcgcata ctgccacgca tcgacgacag
     3181 agaatgcact tgtccggatc cctgacgatc gacggcgtag aatcatcgat agcatggtgc
     3241 gccttggcgc cctcgaacct gatctcccgg atcccaaggg actccgccag agcctgcagt
     3301 tcgcatgacc cactgcgcac gcaggtcaga cattcgaacg gatggttaga tatgatcagt
     3361 tccaacaccg cgcgccgcgc ttcccgcacc tgtctggtgt tcgtgtggac aaccatcccc
     3421 tgtgcagcag gagtaacaca ggatgctgcg agcgttttgg cgccctcgat ctctacaacg
     3481 cagagccgac atgatcctat ctcgttcagc ccctcgaggt agcagagggt aggtatcttc
     3541 gcgcccgcct gcgttgcagc ctcgagaacg ctcgtgcctg ctgggacggt tacttgtcga
     3601 ccgtctatgg tgagagtgac gtcacccatt cgtatcacac tccattctcg tggtcttacg
     3661 gctcgcggac gtcgcactgg aagcagcggg cagcctccgc caccgccatg tcctcagtca
     3721 gaccgagttc gacctctttg aaattcctgg cgcgttcggg aaccggcatc tcgggcatta
     3781 cacacctctt gcactgctct tcgatgggtg tgcccaccag cactctctcg acatcgacat
     3841 cagcatgctg ccacgcggcg cctcccacga acttgtctat ctccaatgca acctggcgcc
     3901 ctgctcctat tgcggcgatc acagtggccg gaccggtgac gcaatcgccc ccggcgaaga
     3961 cacccggcac gttagtctgc atcgtgtcag ggtctgtaat gaccagaccc gacttggtca
     4021 tgagcagcgc ctcctgcccg tcggcaggct tacccgcgaa tgaggtatcg acagactggc
     4081 ctatggccgc gatgactgtg tctacgtcca ctatggtgcg cgacgcctcc acgggcaccg
     4141 gtcgcctgcg acccgacttg tcgaaatcgc ccaacctcat gcgcacacac tccatctgcg
     4201 tgacctttcc atctgcaccg atgagcctgg ttggggcggt tagataatgc acctgaatgc
     4261 cttcctcgat agccgcctcg atctccgcgg gatcggccgg catatccgac cgcagcctac
     4321 ggtagactat gtgaacctct ttggccccca gtcgcagcgc agtgcgagcc gcgtcgattg
     4381 ccgcgttgcc tccgcctact acggctacgt tccgcccgac cttgaccaga cggcccaggg
     4441 ccgcatctcg caggaactcc actgccggca gaaccccttc gagatcctcg ccaggcacgc
     4501 ccattgctgc gctgttgtga gcccctacag acacgaagac cgcatcatac tccgtcttga
     4561 gctggtccag cgtcacgtcc acacccaccc tcgagttgag gcggatgtct acgccggcct
     4621 tcctaatggc ctcgatctcg aactccataa tgtccctggg caaccggtac tctgggattc
     4681 cgacggtcag cattccgcct gccacaggca acgcctcgaa tacggtgacc ttgtggccga
     4741 acagggccag atagtgcgcg gcagacagac ctgccgggcc agctcccaca atggccacac
     4801 acttgccgga aggcggcgcg aaatccgccc tatactggtg cttattcttg atttcccaat
     4861 cggctgcgaa ccgcttcagc gatttgatcg ccacaggttc gtcgaacgtc ccgcgcctgc
     4921 actttgactc gcacggatgg tcgcatacgc gcccgcatat ggcagggaac ggattcgtcg
     4981 cccggatgag gtctaccgct tcatcgaacc tgcgctgctt gatcaaggcg atgtagcgag
     5041 gcacatccac ccccgccggg caggagttct gacacggcga gttgaacagg gccgcgcacg
     5101 acgaagccgg gcactttttg ttccgaatgt gagcctcgta ttcgtctctg aagtaccgga
     5161 ttgtggacat caccggattg ggggcgctct ggcccaggcc gcacatggcg gtagtccgaa
     5221 tgtgttcgcc cagttcgatc aggagttcga tatcgccctc tctgccctgg cccttggtga
     5281 tgcggtccag tatctccagc atgcgcttgg tgccaatccg gcacggagcg cacttgccac
     5341 atgattcgtc ctggatgaac tgcatgaaga atttcgccaa gtccaccata cacgtgtctt
     5401 catccatgac aatgagaccg cctgagccca tcatggtccc tagttccttc agcgactcat
     5461 agtcaatcaa agtgttcagg aactgagctg ggatgcaccc accggaaggc ccgccggtct
     5521 gcgccgcctt gaacttcttt ccgccgggta tgcccccccc gagatcgaag attatgtcgc
     5581 ccagcgcagt gcccatggga acttccacga gaccagtgtt gttgatcttc ccagccagtg
     5641 caaacacctt ggtcccctta ctcttctcag tgccgatgga agagaaccac tccgcgccgc
     5701 gcagaatgat gggcgcaatg ttagcataag tctcaacgtt gtttatgagt gtaggttgac
     5761 cccacaggcc ggacgctgcc ggatacggtg gccgaggtct cggctctccg cggcggctct
     5821 ccaccgacgc cagaagcgca gtctcctcgc cgcacacgaa agcgccggcg cccacacgaa
     5881 gatcgatatc gaaatcgaac ccggtaccga agatgttggc gcccagcagg ccatattccc
     5941 tggcctgcct tatcgcatgc tcaagatgcg ccacagccag cgggtattcg gcccgcacgt
     6001 agatatagcc ctggtttgcg cctaccgtgt accccgctat cgccatcgct tcgagcaggg
     6061 tgtgaggatc gccttccaaa atggagcgat ccatgaaagc tccggggtca ccctcgtcgg
     6121 cgttgcacac cacgtactta ggcgaaccct cagcctttcg ggtgaggtcc cactttatcc
     6181 ccgtggggaa ccccgccccg ccacgacccc tgagcccagc gcgcttcaaa gtgtcgacaa
     6241 tctcttccgg cttcatctct gtaagcgcct tgcccagcgc cgcgtagccg tcgcgggcta
     6301 tatactcgtc gatgttaaga gggtcgataa cacccgtgtt gcgcagagcg attctctctt
     6361 gccggctgaa gaaggtcata gcctcctggg tctctacgac ctcggcagtg cctggttccg
     6421 tataaagaag tcgcttcact atgcgccctt tcacaaggtg ttcatcaacg atctccctgg
     6481 catcgtcggg agtgagcttc ttatagaaga cgccttcggg gtacacgacc atgatcggcc
     6541 ccaggtcgca cggccccatg caccctgtga caaccacctt tatttcgcgc tcaatgcctc
     6601 ttgcggctat ctcagcttcc agcgcctccc gcaccgcctt gcagccggag gagacacagg
     6661 cagcgccgcc gcacacaagc acgtgcgctc gaaccagatc cattcgtatt cctccctcac
     6721 tacgcctgat ggacgtggca ctatactatg aatacttctt gaggacttcc ggcactcgat
     6781 caggctttat ccgcgcgtaa atgtcgtcgt cgactgtcat gactgggcct agcccgcagg
     6841 cgccgataca cctcattacc tggatagaga agagcctgtc gggagtggta tcgcccgcct
     6901 tgatgcccag cgccccttcc accttctcca ggatcttgtc gccgccccgc acatagcagg
     6961 cggtgcccag gcacacacct accttgtgct tgccctttgg cttagtagtg aacagcgcgt
     7021 agaaagtgat gacgctgtac acgtcggcca ccggaatgtc aagtcctgcc gccacccgct
     7081 cttgaacctc aatgggaagg tagcccacaa tctgctggat ttcgtggagg cacgggatga
     7141 gcactccgcg ctgcccctgg tacccggcga tgatctcgtc caccttcgcc cactgctcag
     7201 gtgtgatgtc cgtgatatcg cctgcgcact tggtttcaca gcaacccata gacctttcaa
     7261 cctcctctgc cggccattgc agccacaacc tatacatgca tgattcatgc cacattccaa
     7321 agtggcgttt agctggtgat taccgtagca cacttagaac gcccaatgca tctgtgcatc
     7381 gctagtgaac cgcttcacat tgccttccat agtgaagcgg ggttcatctc gaagcgcata
     7441 agccccatag cagcagtttg gccgctagga acacgcattc ggcacatgcg atgcgtcatt
     7501 gcatcgtgcg atgcatctct gcatcatcca ctgcggccgt tcgcgaatct ctgtatcttc
     7561 ctcactattg tggactggtt gacgcccagg gcacgggcta tcgcataggt gctagggcat
     7621 gttgccgatg cgcgctctat cagctgccgt tccacctctt ccacggcctt gcgcagcggc
     7681 atgatccccg acacgcagac ttccgctgtg ttcttcgccc ccgcactctt gatgtgagtc
     7741 ggaatatgct caatgccgat gacaggggca tccactgtca ccacaaggaa ctccagcacg
     7801 ttctcgagtt ccctcacatt ccccggccag ccatacctct ccagcgcttc tcgagcctct
     7861 ctggatattc ggcgtggcct accgtacctg gcgccccact tctcgaggaa atggtaagtc
     7921 agcggcgcaa tgtctccggc cctctccaca agggacggta ctcggatagg gatcacattc
     7981 agccggtagt ataggtcatt gcggaaacgc ccttccttca ctgcccgctc aatatcgcga
     8041 ttggtcgcag caatcacacg cgcatccacc ctgatctgct ttgtgcctcc cacacggacc
     8101 aggcacttct cttgcagcac ctgaaggagc ttggcctgga gcgccatggg catctcagtt
     8161 atctcgtcca ggaacaacgt accccctgaa gccagctcaa taagcccggg tttgccgctg
     8221 cggcgcgccc ctgtgaaggc tccttcttca tatccgaaca gctcggactc gagcagagtc
     8281 tcaggtatgg ccgcgcagtt gatcttgacg aacggctcgg cgcttcgatt accagttcga
     8341 tgaatatacc tggccaccac ttctttgccc acacccgatg cgccggtaat gagtacagtc
     8401 gatgacacac tcgatacctt ctgcatcaag gcgatgacgc tttccatcgc agggctgtac
     8461 gcaaccatgt ctaccggctc ggagtctgcc gcaaagctgt gggacgccga tcgggcatac
     8521 cggcctcgct ccatcttgcc tgtttggctc gcgtcaggat cgccggaacg agtacctaga
     8581 gcagctcgct ctcgacagtg agccggcgcg gcctccgatg aaaggcttga gaagaacgtg
     8641 cgcgaaagat cgattctgcc cgcgcctgag cacgccaatc gctcaagcac agacgtgccg
     8701 cccgcgctct ccatggcggc ggaatatgcc aacactgcct gacgccgcct ccaggcgccc
     8761 aggttttcag gatacccggg gccatcaacg cagcccctgc aaatcgacaa ctccacaacg
     8821 ggagcagtga tcccgccgga agacatcgca tccaggactt ccatacactc gcggaagcct
     8881 gcagcgttca ctgctccccg ccgaaaggcg ccatccgaag cttccacgct atgaagcaac
     8941 ccggccggca acggcagcac acgcccgggc ccttccgctg ccacatccgg cgctgatgga
     9001 gggcactgat cgacgcgcaa tcccgacttg tcaaacagtt cactcagttc agagaaggtg
     9061 agcaccacat cggccccacc ctctatcctc ctggcagccg ggcttacccg gccgcggcct
     9121 gcgtcgccgc attccagcct aaacgcagca cagggggtaa tgcagacaac ccgcgggcgc
     9181 cctgaggcgc gagccgcccg tgaggcagca atttccggcg acacggccgg cgcgaggttc
     9241 tgcaccagat caggccggtg tgcctcgatc agcctaaccg ccgccggaca cgtcgaggct
     9301 atcacggtgc gccccaaagc cgattccaga gctactcggt acgtggaggc cagcatctcc
     9361 gcccctgccg ccgcttccca cacctcggca aaacccagag ctcgaagcgc cccaatgact
     9421 cgcagcggat catcatctgg aaaggccccg gggaagccgg gcgatatcac tgcagcagta
     9481 tcaccttgtg ccagcaagtc cagcgcaagt tgggtgtggt cggcaagggc aatcgcgcca
     9541 tgagaacact cctctaggca aatgccgcat cctatgcacg tctcgccatc tgcgcgcaca
     9601 gcgccctctg ccgcgtgcat cgcctccaca gggcaggcgc gcacacatgc gtagcatctc
     9661 gtgcagaggt catcgcgtgt ctcgatcagg tccacatcag ccgcccccag tagtaatgca
     9721 taaacgcatc aatggcacaa ggccattata ccatcaccgg cagaagtgcg cacctccagc
     9781 ggcaggtgag ggtgcgtgta acgattgttg ctttcggggt cgcagatacc ccgcattcag
     9841 gcgtgcaatc tggccttgac agttgtggct cagtgatata cccccggggg tatctgccca
     9901 gagatacggt cacattttga caatactttc ggcgaaaaac gcaacttctg agtacatcga
     9961 tttggcccgg ggaccccgga gggcgaatcg gcactatcaa aggagccctc ggggccaatc
    10021 gcaacccagt agctgttact agtgatggca cggcgcgcca tcccgcgcgt gccgttacca
    10081 aatggcccta ttaagtggga tattatgttg gcccgtccgc ctgctttact gcccaaaccc
    10141 gtcggatacc ccccgatttt cgtgattggg aggcggtcag gtggcccttc gaaatcgatg
    10201 tactcaaaag ttgcgggtat ctccgaaact gatggcaaaa atcgacgata tgccagctcc
    10261 agccgttttg cccgacgggc atgatgtcaa gaccagctaa atggccattc cagcgaggcg
    10321 agcgtgaccc ccgcagccaa taacccgccg taatggtcga ggaggatgcc attctcccgt
    10381 gcggcagacg cagacggctc aggcaatctc atcgaatgct gagccaaccg gccatttcca
    10441 aagtccgcca aagccttagg ccgcctccgg atccgaatgc cgtatccaca tccgctctga
    10501 ccagctcgcc gaggccgcct gctcgcgcag tctttgtcgt tctcacacct ctagctgggc
    10561 ctgcagatat ttgcactctc agcccaacgc ctccagaggc aggacaagcc aagcggtgca
    10621 ccgcagggtt gacgggaaaa tacaggtgac tcgtttcgga acacgttttt ggcgatgtgt
    10681 ctggaaaacg aatacaccgc aaagggccag gtaggactac acattaccca tatccatgga
    10741 tgtttctggg tgctgcgctt gcctatgata ggccctgacg ggggttccga ggcatccttc
    10801 gccctggaat gggattacgt ggtctccgat acctgcccat ttggcttaac catattctgt
    10861 gtccggtttt caccggtagc gggcaaaacg tgggggtcta cagggtgtcc tacagggtac
    10921 aggatccggc catagatcac cccggctgcc cggggctctg cccccacttg tttcggcgta
    10981 tccatcggat gtagtataat ccagatagtc gctagcaggt gacaggggga ggcgcgtgta
    11041 gatgcaaaga acccgcgtag ccgtggttgg attgggtaac gtcgggcgag gagctgccga
    11101 ggcattagct gtatcgccag actttgagct tgcaggagtt gtcaggaggc gagcagagcc
    11161 cggtgcgcgc gtcgtcgggc cgaactggga ggcgcctgca gtgacccgaa tggaccagct
    11221 ggaagaggtc cgggccgccc tgatgtgcgg gcccacgcgc gagctggaac cgacggagca
    11281 cgctctgttg cgatcgggta taagcgtggt ggacacatac gacatacacg gagaaccgct
    11341 gtggcgccac aaacagtcgc tagctgcctc agccgccgaa ggtcaggcca cggcggtcat
    11401 agctgccgga tgggatccgg gtattgactc ggtgatcagg gcgctcctcg agatcatcgc
    11461 ccctaccgga atcacctaca ccaatttcgg accaggcatg agcatgggcc actctgtggc
    11521 tgtgcgggcc ataccgggag tggccgatgc ggtgtccgtc actctccccg caggctacgg
    11581 aaggcacaga cgcgctgtct acgttgagct cgacgggtcg cggccttttg ccgatgtcgc
    11641 gtccgacatc cgttgtgatc cctacttcgt tcgtgatgag accgatgtgt tcgaagtgca
    11701 cagcgcgagc caggcggccg atcccgggca cggtgtcgta atcgatagaa agggcatcgc
    11761 gggccgcacc gggaatcagc tgctcaggtg ggagatgcgg ataaccaacc cggccgttac
    11821 cgggcagatc atggtaggtg cgctcagggc agggctaaag aaggcgccgg gcgcgtacac
    11881 catgatcgag ctggcggcgg ctgacatgct gccggggacc ctagaggact ttgtgaagag
    11941 gatggtctga agtggttcag gcctaggcgg ggcagcgggc ttccatcacc ctattcttgc
    12001 cctggcgctt agcctcgtac atggccgcgt ccgcggctct cacaagggca tcgacggatc
    12061 cgccggactg gggcatggaa gccacgccca ggcttatggt agccgtcacc ggcgtgccat
    12121 cgaccataag cggcgttcgc tccatctcgg cccggagcct ctccgcgagc accagtccgt
    12181 cggctccagc agtattgggc aggattatca tgaattcatc tccggcgtat ctcgcgagta
    12241 tgtcggactt ccgggccacg gctctcatcg tgtcggcaag gtgaatcagg tgagtatcac
    12301 cgatggcgtg cccatacttg tcgtttatgt acttcaggtg atcagaatcc agcatgacga
    12361 gcgtaagggg agtactgtgc cgaatagctc tttcgatctc cttgtcgaga agatcgcaga
    12421 agtacctgta gtttcccagc ccggtaaggt catcagtgag cgataactcg cgggctgatt
    12481 ggtacaaccg agcattctca agggctatgg cagcgctgga cgcgatcgtc atcaggagat
    12541 ctctctcctc ctcaccgtag gcgttcatcg tatagctctg cgccgaaacc gcgcccacca
    12601 catgagcgcc tacgcgcatg ggcacaatga tcacagatgc tgtaacctca tcaacgttgt
    12661 gcccaagcct acgctccccg tccatcattt cgtcttcctg gcggttgaac agaatcggct
    12721 ggccacttgt gatcaccttt gccgtcggac ccgattccat cccaaaccgc gtcgaaggag
    12781 aacgaactcc ttggtccatg agaaacgcca tttcgatctc gcgacttcca gcgtggaata
    12841 tcgccacaaa aaacgcgtca acagggatta tccggcagac ttcgcgatag atcttttcga
    12901 aaagcgcgtc tcgttcgaga accgatgtga ggctcatgga tatctcgttg agagtggaca
    12961 gatagtccgc ccttcgctgt acctcatcca gtaggtgggc gttccccaca accacgctca
    13021 gctgtttccc cagcatctcg aggaactcac agtcttccgg cgtatacgcc gcgcccgacc
    13081 gcctattgcc gaaggtaagc gctccaatgc agtcaggacc ggatagcaga ggagtggcgg
    13141 caaccgcact taatccttct gcaaccactt ccggaatggc acccgaaaac gacgcgtagt
    13201 cggatactcg gaccgtctgc cgttgcgcaa cggccacgtt gaccagtccc tcatctttcc
    13261 taaagacgcg acctaggagc ccgggtggtg agttcctgca ggcggacgcg actagtctcc
    13321 cgcgccggtc cttgagagcg accatgccga agtcgccgcc aagcacctcc aagcctccgt
    13381 ctagaatcag ttcaagggtc gctctcagcc cgattccgga gttgatcttg cgggtgacac
    13441 gcgccagaac ctggtcccag tgggcccgcc tgtcaagctt gtcgcgggct gacgccagtc
    13501 gcgcagcaca ctccttcgac gcctgcgaca tgaggccaat tgccccgccc gcgaacataa
    13561 taacgccaca tctgtacggg agcgtccatc caggtatggc cggctcctgc gaccaaggaa
    13621 atgtagccgc gccgtagagc gcagcgcaca caataccgcc caaggagctg gaaatcgcgc
    13681 tttcctgcaa cgcgataatg gaaagcgcca cgaagtaaag cgcgtatgcc tcgcttgaga
    13741 gacctccgcc aaaatgcacg cagacggata tgaatcccat gtcgaaggcc gacgccacaa
    13801 cgctgactac gcgggagttg aacctgctgc ccagcatcca tatggcgacc gagagcgccg
    13861 cggcgggtat tacggtagta atcagtcctg gccgtattgc cagcttatga aggccgaaca
    13921 tgatgcatgc agcaacgatc actgaacgaa gcaagatgat ggcaatgcca gccagacgcg
    13981 catgct
//
LOCUS       DAOTAC010000024        13782 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_3757_length_13782_cov_5.311284, whole genome shotgun sequence.
ACCESSION   DAOTAC010000024 DAOTAC010000000
VERSION     DAOTAC010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 13782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13782
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3757_length_13782_cov_5.311284"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..1053
                     /gene="rbsK"
                     /locus_tag="PLM74_01955"
     CDS             55..1053
                     /gene="rbsK"
                     /locus_tag="PLM74_01955"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HON41879.1"
                     /translation="MQPRIVVVGSLNMDIGLGVKHVPRPGETTAALSARFSPGGKGAN
                     QAVAAAQLGAHVSMVGSVGADTQGDELVRNLREAGVCVDRIAREQGSTTGMAVIVVDE
                     AGQNSIVVWPAANGTLSADRVRAAQDVISEARALVVQFETPMAGVEEALAIARRHGVL
                     TVCNPAPARHVDAQFLGKVDILIPNEVEASMISDVFVQDMSSAMRAAESIRSRGAARV
                     VVTLGENGAIYAGPEGRLHCPAFSVRSVDTTGAGDAFVAGFAVAWLSQPDPWHSLQYA
                     CAVGAIVTQTMGAQSPALSMENVQELLRAARGVGDAHGARGQGGDTGIASRHTMDL"
     gene            1131..2474
                     /locus_tag="PLM74_01960"
     CDS             1131..2474
                     /locus_tag="PLM74_01960"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HON41880.1"
                     /translation="MTTKNTWLKAVLCLAALLAMALAPIAAHAASAPVKIKFWIEQTQ
                     DIAMQKMVEWFDLFNASQNEVRVEIRSAGEGDEYLQSVRAALASGSDIDMWRLFGPST
                     MPPFADAGLLLDLTPYAAKYNWKDRFFSYALESHSYKGKLMAFPANPETLLLYYNKTE
                     FDKRGWKVPTTWEELCATCENIQKIGRIPIAFGVRGFTPANEWWLSVVFNSAAGPENV
                     YKALTGQIPWTAQPFVDSIAKLKYMWDKGWIMNKQMYDVTSDDAVAVWGTQKALMMMD
                     GSWGVKYAPLYAKGFDWALAPFPAMGAASPTLPLALGFSVGVNAKTKHPDAVAKFLNW
                     LTENPKTAVEYGINSNLEFWLPLKKAAPDYLPATTAPKTADMYRTIYEAQSSGKYGYA
                     TWTFWSPGTQLYMYEKLPRVLLGLMSIEDYLKEAQSIFTQELAAGKVPPVPAPAK"
     gene            2588..3460
                     /locus_tag="PLM74_01965"
     CDS             2588..3460
                     /locus_tag="PLM74_01965"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HON41881.1"
                     /translation="MRKCRLGKRAMAALFCLPAAALVIVVIGVPSISSLAMSLFEWDG
                     LSKPAFVGLRNFADILFRDKEFRIGLVNNLKWTVIFMIVPIFLGLVIAFALASIKRMR
                     LVYMTVLFIPVVLSRVVVARLWGWVFNPFFGINTLFEKLGWTALCQSWLGNPHIALYS
                     LAFVDNWTWWGFVMVIFLAAIQQIDPAYYEAAVIDGASTAQKFRYITIPLIRPTLNFI
                     LLLTAVWSLSAFDFSYIMTQGGPGHATEMMSTWIYKQGILNFRVGYASAMSVLLLAFS
                     LLVILLFSAVRRDA"
     gene            3460..4305
                     /locus_tag="PLM74_01970"
     CDS             3460..4305
                     /locus_tag="PLM74_01970"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HON41882.1"
                     /translation="MVTRSKHRGEVRHQALLHAILIVACLVVLVPLFYLVINSFKDNY
                     EVILSPFALPSAPTIENYRKAWTTGGLAEAMLNTVLVVAVSTVVLAVVSCMMSYGMVF
                     LGVRSTPVWSALLMLAMQVPLHMYIVPLFVMWKRLGLVNNPLGIILIYAAVYVPFSVL
                     FLNSQFASIPKEITEAAEIDGCSYIGTLTRVVVPISRPALVTLTLINAKWIWNEFTFA
                     MTFLQEPRFQTVTARYLQFSARFVADFAVTGAGAVISVLPIVVLYLLLQRRFIEGMTA
                     GSIKG"
     gene            4342..5640
                     /locus_tag="PLM74_01975"
     CDS             4342..5640
                     /locus_tag="PLM74_01975"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HON41883.1"
                     /translation="MTHAEKQAAEMWQDIMTYSRTVENRMIECVDALEKRGYDVSEAR
                     RLIPAGWEAYNSGDYWALQKVIAMIWEALRRAPRMVNDDHKPDTWEELKRSWIRVESP
                     MIFNVDEGNRAYRDKVYGAWYGKCIGCALGDPSAGWPSAKVRKERGWVGDYLQKPDTR
                     NDDINYQIIVLHCIDEHGPGFTSRDLGYEWVQHLELDKTYTAERQALENLHRGLIPPY
                     SARENNPFSDWIGAQMRGEVHGLIAPGNPELAAELAYRDAIISHVKEGVYGEVFNSVM
                     VSLAFVTDDVGEIVNRALGYVPANSDFASVVRSTVAKCKEHGRWEDVLDWIDQAYGDL
                     HWIHTFPNAAIVVMSLLLGGGDFSKSVSMAASCGWDCDCSTGQVGATVGALIGERAIP
                     ARWKEPIAGRLDTDVIGFSSIELDKLTDWTCNVARRLAQA"
     gene            5669..7195
                     /gene="hutH"
                     /locus_tag="PLM74_01980"
     CDS             5669..7195
                     /gene="hutH"
                     /locus_tag="PLM74_01980"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007935211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HON41884.1"
                     /translation="MGVCIDGAGLSIPDVVAVARGGAPVSLDLDARRRIDRASAYVDT
                     LLRRERPVYGVTTGFGRFADVVISPDDSAALQRNLLLSHASGVGELLPDEVVRAILLL
                     RANALACGYSGARAEVVDTLIAMLNQGVHPLVPSQGSVGSSGDLAPLAHTMLVVIGEG
                     MARYKGEILPGAEAMARAGIPVVALRAKEGLALINGTQVMTAIGSLAVHDVSILAQVA
                     DIAVAMTTEALRGTTSAFDPRVQAVRPHPGQAASARNLLRLMEGSAIRESHRNCPKVQ
                     DAYSLRCAPQVHGAARDAIEYARQVVEREMNSATDNPLVFPEDGDVISGGNFHGEPVA
                     MAMDFLGIAASELASISERRTERMVNPQLSGLPPFLTEHGGLNSGMMIAQYTAAALVS
                     ENKILASPAVVDSIPTSGNQEDHVSMGTIAARKARQIAANVANVLSIEVMCAAQGIDF
                     LAPLEPGAGARAAHEAVRAQVPHLDHDRVLAPDIALIRELIQGEALLRAVEHAVGQLE
                     "
     gene            7257..8906
                     /gene="hutU"
                     /locus_tag="PLM74_01985"
     CDS             7257..8906
                     /gene="hutU"
                     /locus_tag="PLM74_01985"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002194617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HON41885.1"
                     /translation="MSRVIRAPRGTEISCKGWQQEAALRMLMNNLDPDVAERPDDLVV
                     YGGRGKAARNWEAFDAIVRALRGLESDETLLVQSGKPVAVFRTHEDAPRVLISNAMLV
                     PHWATWEKFWELEAMGLTMYGQMTAGSWIYIGTQGILQGTYETFAAAARKHFGGSLRG
                     KLVLTAGLGGMGGAQPLAATFNDAVMIAVEVDRSRIDRRIATKYCEMVTDSIDEAIAL
                     AREAQERGIPRSIALVGNAAETHPEFVRRGVIPDLVTDQTSAHDPLNGYVPAGLTLEQ
                     AAEMRKSDPDQYVRRARSSMATHVRAILDMIAQGAVAFDYGNNLRQVALDEGVTNAFD
                     YPGFVPAYVRPLFCEGKGPFRWAALSGDPKDIWVTDEVILKEFAYDEHLVKWIQLAHD
                     KVAFQGLPSRICWLGYGERARFGRIINDLVAEGRISAPIVIGRDHLDCGSVASPNRET
                     EGMKDGSDAIADWPVLNAMLNAVNGATWVSFHHGGGVGIGYSLHAGMVIVADGTEEAG
                     RRLERVLTGDPGIGVVRHADAGYEEAQEFAREHGIKIPEVG"
     gene            complement(8884..9849)
                     /locus_tag="PLM74_01990"
     CDS             complement(8884..9849)
                     /locus_tag="PLM74_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HON41886.1"
                     /translation="MERTTRERTTLEKTTLEKTILEETTLEHTTPMEPGEIIAQVNCL
                     KHVYPDQTTVDLCGLDFTVRRGQRVAILGANGSGKSTLIFHLLGLLSPVDGQVTVFGV
                     NPAKQFRDIRHRVGVLLQNVDDQIIGPTVWDDVTFTPRALGWPRSQVEARGNAICERL
                     EISHLKTRIPHYLSGGEKRKVALAGALVTEPELLIMDEPFTGLDPRSRSELVRLMNQI
                     HSEYGVTYVISTHEIDFVPHIANWVYVLGGQHGITLSGTPEQVFSQPEALRAVSIDLP
                     ALGELFEALQEKGWDVSVPLTVDQAVAELEKCRLDAAAPTLLRES"
     gene            complement(9879..10583)
                     /locus_tag="PLM74_01995"
     CDS             complement(9879..10583)
                     /locus_tag="PLM74_01995"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HON41887.1"
                     /translation="MDVQFIDELAANGRTPLHDALPWVKIAASATLLAASIAARTPSA
                     AAAAAAAVVMFASASRNQLRHAVAMALYPVFFAGLFALSWIRSNPLLALTVVIRSFSA
                     GLVAATLITTTPFVDVFAAISRVMPAIVSDALFMAYRSFFILVDELQDLLRAVRLRGG
                     AGRSIMEQLRTYGQVLGVVILHSADMTERMYRVMIVRGYSQRIQSAREWPPPRPLDYL
                     LIAYAMTVLGLVLALR"
     gene            complement(10588..11346)
                     /locus_tag="PLM74_02000"
     CDS             complement(10588..11346)
                     /locus_tag="PLM74_02000"
                     /inference="COORDINATES: protein motif:HMM:NF014007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HON41888.1"
                     /translation="MSHLHIPDGLIPLPWILAGIGLTVLLVCVALRALSMRDRARVVP
                     RIATLSALMLLAMGLPIPVLGYHLNLTVLTGMIAGPAEGFIAALVVNLILALTAHGGI
                     TVVGLNALIGGLEVCLGWLLWRSCRRVFRRPFAAAAIATVVALLISTTAMLGIVAGTR
                     INPDLALHRHSHAELAEHTEPADDHEHAELEEPADHEHAPGSDVGSLKAFAAIVYSAG
                     AVGWAVEALVVGSIAEFITKSRPDMLDPGSGRRV"
     gene            complement(11350..11856)
                     /locus_tag="PLM74_02005"
     CDS             complement(11350..11856)
                     /locus_tag="PLM74_02005"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HON41889.1"
                     /translation="MGIVIESGFHLRLGGAQVQADCFDDALHKLRERGFKLTPQRRAV
                     LELFYSDGAQHLTAQQAHDAIRGREPGIGLVTVYRCLELFTSVGILDKIKFNDGFDRY
                     ERRPDPSGHHHHLVCTGCGQVVEFGDCLIRSIEHELERMSGFCIEGHWLELIGQCPRC
                     RERAKEDN"
     gene            13572..>13782
                     /locus_tag="PLM74_02010"
     CDS             13572..>13782
                     /locus_tag="PLM74_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014187212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HON41890.1"
                     /translation="MDSGQGMRVVECVPNFSEGRRPEVVAEIVSAITSVEDVKLLDFS
                     SDASHNRSVVTFVGTPEVVEAAAFAG"
BASE COUNT         2810 a         3813 c         4366 g         2793 t
ORIGIN      
        1 gccgcagttg gtgctgaggg aatcttacgc aacgatgaaa tgcgagggat cgggatgcaa
       61 cccaggattg tagttgtggg tagcctgaac atggatattg gtctgggcgt gaagcatgtt
      121 cccagaccgg gggagactac ggctgcgcta tcggcccgat tctcaccggg cgggaaaggc
      181 gcaaatcagg ctgtggccgc cgctcagctg ggagcgcacg tgtctatggt gggcagcgtg
      241 ggggcggata ctcagggaga tgagcttgtt aggaatctta gggaggctgg tgtgtgtgtc
      301 gaccggatcg cgcgcgaaca gggctcgacc acgggaatgg ctgtcatagt ggtagacgaa
      361 gccggccaga attcgatagt ggtgtggccg gcggccaacg gcacactgtc cgcggaccgg
      421 gttagggcag cccaggacgt gatctccgag gcacgcgccc tcgttgtcca attcgagacg
      481 cctatggccg gcgtggagga agccctcgcc atcgctcggc gacatggcgt gctgacggtg
      541 tgcaatccgg cccctgcccg gcacgttgat gctcagtttc ttggcaaggt cgacatcctc
      601 atacctaacg aagtggaggc gtccatgatt tcggatgtat tcgttcagga tatgtccagc
      661 gcaatgcggg cggctgagag tattcgatcg cgcggggcgg ctagagtggt tgtcaccctt
      721 ggcgagaacg gagcgatata cgcggggcca gagggaaggc ttcactgccc cgcattcagc
      781 gtcagaagtg tagatacaac tggtgcaggc gacgcttttg tagcaggatt cgcagtggcc
      841 tggctctcgc agcctgaccc gtggcacagt ctgcagtacg cgtgcgcagt cggagccata
      901 gtcacccaga ccatgggagc acagtcgcct gccctttcta tggagaacgt ccaggaacta
      961 ctgcgcgctg cgaggggcgt cggggacgcc catggcgcgc gcggccaggg aggagatacc
     1021 gggatcgcca gccggcatac catggatcta tgaggttcga gtgtcgcggt accagacgta
     1081 ccaatgcatc caccatgcaa tcatggcaaa tgggaggttt gaacgcagaa atgacaacaa
     1141 agaacacatg gcttaaggca gtgctctgcc tggccgctct gctggccatg gccctggcgc
     1201 ccattgctgc tcatgcagca agtgctccgg tgaagatcaa gttctggatt gagcagacac
     1261 aggacatagc gatgcagaag atggtcgagt ggttcgatct gttcaacgcg agtcagaacg
     1321 aggtgcgtgt ggagatcagg tcggcaggcg aaggcgatga gtatctgcag tctgtgaggg
     1381 ccgcccttgc ttctggttct gacatcgaca tgtggaggct attcgggccc agcacaatgc
     1441 cgccattcgc agacgcgggc ctccttctcg accttactcc ctacgcggcc aagtacaact
     1501 ggaaagacag gttcttcagc tacgccctgg aaagccacag ttacaagggg aagctgatgg
     1561 ccttcccggc gaacccggag actcttctcc tgtactacaa caagaccgag ttcgacaagc
     1621 gcgggtggaa agtcccgacc acttgggaag agctgtgtgc aacgtgcgag aatatccaga
     1681 agatcgggcg tatcccgatc gccttcggcg ttcgcggctt cactcccgcg aatgagtggt
     1741 ggctatccgt ggtgttcaac agcgccgcgg gcccggagaa cgtgtataag gctctgacgg
     1801 gtcagatacc gtggacggcc cagcccttcg tagattccat cgctaagctg aagtacatgt
     1861 gggacaaggg ctggatcatg aacaagcaga tgtacgacgt taccagcgat gacgccgtcg
     1921 ccgtgtgggg cactcagaag gccctcatga tgatggacgg aagctggggc gtgaagtacg
     1981 cgcccctcta cgccaagggc ttcgactggg cacttgctcc gttcccggcc atgggcgccg
     2041 ccagccccac tctcccgctg gcgctgggct tttcagtcgg agtcaatgcg aagaccaagc
     2101 atcctgacgc cgtggccaag ttcctgaact ggcttaccga gaaccccaag acggccgtgg
     2161 aatacgggat caactccaat ctcgagttct ggctacctct gaagaaggcc gctccggatt
     2221 acctgccggc aacgaccgct ccgaagactg ccgacatgta cagaaccata tacgaagctc
     2281 agtccagtgg gaagtacggc tacgctacct ggacgttctg gtcgcccgga acgcagctgt
     2341 acatgtatga gaaactgcct cgcgtgctcc tggggctgat gtccatcgag gattacctga
     2401 aggaagctca gtccatcttc acacaggaac tcgcggcggg gaaggttccg cctgtaccgg
     2461 cgcccgctaa gtaggaggac tgactctacc aatagctgca gtttgaaggg cgcggcggcg
     2521 accggccggc acggccgacc atacatcgcc gctgcgccta cggcgcaggc gaaggagttg
     2581 tcttcggatg agaaaatgca gacttggcaa gcgggctatg gcggctctat tctgtcttcc
     2641 ggccgccgct ctggtgatag tcgtcatcgg cgtaccctcg atttcgagcc tggcaatgtc
     2701 ccttttcgag tgggatggcc tgagcaagcc ggcttttgtg gggctccgga attttgccga
     2761 cattctgttt cgggacaagg agttcaggat aggtctcgtt aacaatctga aatggactgt
     2821 catcttcatg atagtcccaa tctttctggg gctcgtgatc gcgttcgcgc ttgcgtcaat
     2881 caagaggatg cgcctggtgt atatgactgt cctcttcatc ccagtggtgc tgtcacgggt
     2941 agttgtggcg cgcctatggg gatgggtctt caacccattc tttggcataa acacgctgtt
     3001 cgagaagctc ggatggacgg ctctctgcca gtcatggctg gggaacccgc acatcgccct
     3061 ttactcgctg gcctttgtgg acaactggac gtggtggggg tttgtgatgg tgatcttcct
     3121 cgccgcaata cagcagatag accccgcgta ctatgaggcg gcagtgatcg acggcgcgag
     3181 tacggcacag aagtttcgct acatcacgat tccgctgata aggccgactc ttaactttat
     3241 cctcctgctc actgcagtgt ggtctctatc tgccttcgat ttcagctaca ttatgacaca
     3301 gggcgggccc ggacatgcta ccgaaatgat gtctacatgg atatataaac aaggtatcct
     3361 caatttccga gtaggctatg cgagcgccat gtcggtgctt ctgttggcct tttcgctcct
     3421 ggtaatcttg ctcttctcgg cagtcaggag ggatgcctga tggttacgag atccaaacac
     3481 agaggcgagg tgcgacatca agcactgctg catgcgatac tgatagttgc ctgtctggtt
     3541 gtgctggtgc ctctgtttta cttggtcata aactccttca aggacaacta cgaagttatc
     3601 ctatcgcctt tcgcactgcc ttcggccccc accattgaga actacagaaa ggcctggacc
     3661 accgggggtc ttgcggaggc tatgctcaac accgtcctgg ttgtggcagt ctccactgta
     3721 gtgctagctg tcgtatcgtg catgatgtca tatggaatgg tgttccttgg cgttcggtcc
     3781 acacccgtat ggtctgcctt gctcatgttg gccatgcagg taccgctgca tatgtacatc
     3841 gtgcctctgt tcgtgatgtg gaaacggctg ggtcttgtca acaacccgct agggatcatc
     3901 ttgatatatg cagcagtcta tgttccgttc tcggttctct ttctgaactc gcaatttgcg
     3961 tcgattccaa aggaaataac ggaggcggct gagattgacg gctgctcata tataggtaca
     4021 ctgaccagag ttgtagtccc catatcccgt cctgcgctgg tcaccctgac actgatcaac
     4081 gctaagtgga tatggaacga gttcactttt gccatgacat tccttcagga accgcggttt
     4141 cagacagtca cagcccgata tctgcagttc agcgccagat tcgtggctga ctttgctgtg
     4201 accggggcag gagcggtaat cagcgtgcta ccgatagtgg tgctgtacct gctgcttcag
     4261 cggcggttca ttgagggaat gacagcgggc agcatcaagg gttaacaggt tatcaagtct
     4321 cgccaaggga ggaataccag tatgacgcat gctgaaaaac aggcggccga gatgtggcag
     4381 gacataatga cctacagccg cactgtggaa aatcggatga tcgagtgcgt ggatgccctc
     4441 gagaagcggg gttacgatgt gtcggaggca aggcggttga ttcctgcggg atgggaggcg
     4501 tacaattccg gcgactactg ggcacttcag aaagtgatcg ccatgatctg ggaggcgttg
     4561 cgccgggccc ccagaatggt gaacgacgac cacaagcccg acacatggga agaactcaag
     4621 agaagctgga ttcgtgtgga gagccccatg attttcaatg tggatgaagg gaatcgggcc
     4681 tatcgtgaca aagtatacgg cgcgtggtac ggtaagtgta taggttgtgc cttgggcgat
     4741 ccgtcggccg gatggcccag cgcaaaagtg cgcaaggagc gcggttgggt cggcgactat
     4801 ctgcagaagc cggacacgag gaacgatgac atcaactacc agatcatcgt gttgcactgc
     4861 atagatgagc atggacccgg ctttaccagc agggatcttg ggtacgaatg ggtacaacat
     4921 ttggaactgg acaagaccta caccgccgag agacaggcgc tggagaactt gcacagaggc
     4981 ctcataccgc cgtattcagc gcgtgagaac aatccgttca gcgactggat tggagctcag
     5041 atgcgcggcg aggtgcacgg tcttattgcc cccggcaacc ctgaactcgc agctgagctg
     5101 gcgtacaggg atgcgattat ctcgcacgtg aaggaaggcg tgtacgggga ggtcttcaac
     5161 tcggtcatgg tcagtctggc gtttgtgacc gacgacgtgg gcgagatcgt caaccgggcg
     5221 ctcgggtatg tccctgcaaa cagcgatttc gcgtcagttg tccgatcgac tgtagctaaa
     5281 tgcaaggagc atggccgatg ggaggatgtg ctcgactgga tcgaccaggc ctatggcgat
     5341 ctgcactgga tacacacgtt cccgaacgct gctatcgttg tgatgagcct gcttttggga
     5401 ggcggcgact tcagcaagag cgtatcgatg gcggccagct gtggttggga ctgcgactgc
     5461 agtaccgggc aggtgggggc cactgtcgga gcgctcatag gcgagcgtgc catccctgca
     5521 aggtggaagg agcctatcgc cgggcgtttg gatactgatg ttatcggctt ttccagcatc
     5581 gagcttgata agctaaccga ctggacttgc aatgttgcgc ggaggctggc ccaggcataa
     5641 cgtgaacgaa gggactgaag ctgctgcaat gggtgtatgc attgacggcg ccggcctatc
     5701 cataccggat gttgttgcgg tcgcgagagg cggtgcgccg gtatcgcttg acctcgacgc
     5761 acggcgacga atagaccggg cctcggccta tgtggacaca cttctcaggc gcgaaaggcc
     5821 agtgtacggg gtaacgaccg ggttcggcag gttcgccgat gttgtcatct cgcctgatga
     5881 ctcggcagct ctgcagcgaa acctgttgct gtcgcatgcg tcgggagttg gcgaactcct
     5941 gcccgacgag gtggtccggg cgatcctgct gcttcgggcg aatgcgctgg cgtgcggata
     6001 ttcaggcgct agggccgagg tcgtcgacac tctcatagcc atgctcaacc agggcgtgca
     6061 ccctctcgtc ccgtcccagg gttcggtggg ttcaagcggc gacctggcgc cgcttgccca
     6121 taccatgctg gtggtcatag gcgagggcat ggcacgttac aagggcgaga ttctgccggg
     6181 agcggaggcc atggcgcgcg cggggatccc tgtggtcgct ctgcgcgcta aggaaggact
     6241 ggccctgata aacggcaccc aggtcatgac cgccataggt tcgcttgcgg tgcatgacgt
     6301 gtcgattctc gcgcaggtgg cggacatagc ggtggcaatg accaccgagg ccctacgggg
     6361 caccacatcc gccttcgacc cgagggttca agcggtgcga ccccatccgg ggcaggcggc
     6421 gtcggcccgc aacctgctcc gtctgatgga ggggagcgct atccgcgagt cgcacaggaa
     6481 ttgcccgaag gtgcaggacg cctattcgct ccggtgcgca ccgcaagtgc atggagctgc
     6541 gcgcgatgcc atagagtacg ccaggcaggt ggtggagcgg gagatgaact cggccaccga
     6601 caatcctctg gtcttccccg aggacggcga tgtgatatca gggggaaact tccacgggga
     6661 gcccgtggct atggccatgg acttcttggg gatagcagcg tctgagctgg cctccatatc
     6721 cgagcgtcgt actgagagaa tggtcaaccc tcagctgtca gggttgccgc cattcctcac
     6781 tgagcacggc gggctcaact caggcatgat gatcgcccag tataccgcgg ccgcgcttgt
     6841 ctccgagaac aagatactgg catcgcctgc ggtagtcgac tcaataccca cttcgggtaa
     6901 ccaggaggat catgtgagca tgggcacgat cgccgcgcgc aaggcgaggc agatcgctgc
     6961 aaatgttgcg aacgttctct ccatcgaggt aatgtgcgcc gctcagggca tcgacttcct
     7021 ggctccgctt gagccgggcg caggcgctcg ggcggcacat gaggctgtca gggctcaggt
     7081 accgcacctt gaccacgaca gggtgctcgc gcctgatatc gcccttatcc gagagctgat
     7141 ccagggggag gctctgctgc gagcagtgga acatgcagtg ggtcagctgg agtgagctgc
     7201 gctggacggt atgaccgtaa gtatagacat agatgtacgg ctggagggat caatggatgt
     7261 caagagtcat aagggctccg cgcgggactg agatctcgtg caaggggtgg cagcaggaag
     7321 cggcgctcag gatgctcatg aacaacctcg accctgatgt ggctgaacgg ccggatgact
     7381 tggtggtcta tggaggtcgg gggaaagccg cgcgcaattg ggaggcgttc gacgccatcg
     7441 ttcgggcgct tcgcggcctc gaatccgacg aaacgctact tgtgcagtcg ggcaagccgg
     7501 ttgcggtatt cagaacacac gaggacgcgc cgcgcgttct gatttccaac gccatgctcg
     7561 tgccgcactg ggccacctgg gagaaattct gggaactcga agcgatgggc ctaactatgt
     7621 acgggcagat gaccgccggc agctggatct acatcggcac ccagggaata ctgcagggaa
     7681 catacgagac ctttgcggcg gcggcgcgca agcatttcgg aggtagcctc aggggcaagc
     7741 tggtgctgac ggcggggctt gggggaatgg gtggtgctca acctctcgcg gcgacgttca
     7801 acgatgcggt gatgatagca gtcgaagtgg ataggtccag aatcgaccgg cggattgcca
     7861 cgaagtactg cgagatggtc accgacagca tcgatgaagc catagcgctg gcccgcgagg
     7921 cgcaggagag gggtatcccg cgatccatcg cgttggtggg caatgcggct gagacgcacc
     7981 cggaattcgt acggcgcggc gtgatacctg acctggtgac cgaccagacc tctgcgcatg
     8041 acccgctcaa cgggtatgtt cctgccgggc ttacgttgga gcaagctgct gagatgcgca
     8101 agtccgatcc tgaccagtac gtgcggaggg cccggtcctc catggccacc catgtcaggg
     8161 cgatactcga catgattgca cagggcgcgg tggcgtttga ctacggcaac aacctgcgtc
     8221 aggttgcgct cgacgagggc gtgacgaatg cgttcgatta ccctggattc gttccggctt
     8281 acgtgcgtcc gctgttctgc gaggggaagg gtccgttcag gtgggcagcc ctttcaggag
     8341 accccaagga catctgggtc accgatgaag tcatcctgaa ggaattcgcc tacgacgagc
     8401 acctcgtgaa atggatccag ttggctcacg acaaggtagc attccaggga ctgccgtccc
     8461 gcatatgctg gctgggctat ggcgagcggg ccaggttcgg gcggattatc aacgacctgg
     8521 tggctgaggg tagaatcagc gcgccaattg tgatcggccg cgaccatctc gactgtgggt
     8581 cggtggcgtc gcctaaccga gagactgaag gcatgaagga cggctccgac gccatcgccg
     8641 attggccggt gcttaacgcg atgctcaacg cggtcaacgg cgccacctgg gtgtcattcc
     8701 accacggcgg cggggtcggc atcggttact cgctccacgc cggaatggtc atagtggccg
     8761 acggcactga ggaggccggg cggcgcctag agcgcgtgct tacaggcgac ccaggaattg
     8821 gcgtggttcg ccatgccgac gccggctatg aggaagccca ggagttcgcg cgcgagcatg
     8881 ggatcaagat tcccgaagta gggtaggcgc ggcggcgtcg agcctgcact tttccagttc
     8941 ggcaacggcc tgatctacag tgagtgggac ggaaacgtcc cacccttttt cttgcagcgc
     9001 ctcgaacagc tctcccagcg cgggaaggtc aatgctcacc gcgcggagcg cttccggctg
     9061 ggaaaacacc tgctcgggcg tgcccgacag ggtaataccg tgctggcccc cgagaacgta
     9121 gacccagttg gctatgtgcg gcacgaagtc gatctcgtgt gtggagatta catacgtgac
     9181 cccgtactcc gaatgaatct ggttcatcag ccggactagt tccgaacgcg accttgggtc
     9241 gaggccggtg aaaggctcgt ccattatgag cagttcgggt tcggtaacga gcgcgcccgc
     9301 cagcgccacc ttgcgcttct cgccgccgct caggtaatgc ggaatcctgg tcttaaggtg
     9361 cgatatctcc aggcgctcac agatggcgtt gccgcgtgct tcgacctggc tgcggggcca
     9421 gcccagagcg cgcggagtga aggtgacgtc atcccacaca gtagggccga tgatctggtc
     9481 gtcgacattc tgcaacagca cgcctacgcg gtgacgtatg tcgcggaact gcttcgccgg
     9541 gttcactccg aacacggtca cctgcccgtc aacgggggat agaagcccga gaaggtgaaa
     9601 gatgagcgtg gacttcccgc tgccgttggc cccaagtatg gctactcgct ggcctcgacg
     9661 gaccgtgaag tccagcccgc acaggtcgac ggttgtctgg tcagggtata cgtgcttaag
     9721 gcagtttacc tgcgctatta tctcgcccgg ttccataggg gttgtatgct ccaaggttgt
     9781 ctcctccaag attgtcttct ctagggtggt cttctccaag gtcgttctct cccgggtcgt
     9841 cctctccaag gtcgacccat ccaaggttct gcccgcgatc atctgagcgc cagcaccaga
     9901 ccgagcacgg tcatggcata tgcaatcagc agataatcga gaggcctggg cggaggccat
     9961 tcacgcgccg attggattcg ctgcgagtat ccgcggacta tcatgactcg gtacattcgc
    10021 tcggtcatgt cggccgaatg caggatcacc acacccagga cctggccgta cgtgcgcaac
    10081 tgctccatga tgctgcgccc tgcgcctccg cgcagacgca cagcgcgcag caggtcctgt
    10141 agttcgtcta caaggatgaa gaacgagcgg taggccatga agagcgcatc cgagactatc
    10201 gccggcatca cccgggagat ggcggcgaag acgtcgacga agggcgtcgt agtgatcaac
    10261 gtggcagcga ccaggccggc cgaaaaggac cggatcacca ccgtcagcgc gagaagaggg
    10321 ttagaacgaa tccagcttaa ggcgaacagg cccgcgaaaa acaccgggta gagtgccatt
    10381 gccactgcat gacgcagttg gttacgtgac gccgacgcga acatgaccac ggcagccgcc
    10441 gcagcagcag ccgccgaggg cgtgcgggcg gctatcgatg ccgcgagcag ggtagccgag
    10501 gcagctatct tcacccaggg tagtgcgtca tgtagcggtg tgcggccatt cgctgccagc
    10561 tcgtcgatga actgcacatc catgagccta gaccctcctg ccggagcctg gatccagcat
    10621 atccggcctg gacttggtga tgaactcggc aatcgacccc actacgaggg cctcaacagc
    10681 ccatcccact gctccggctg agtacacaat agcggcaaag gctttcagcg atccaacgtc
    10741 gctgcccggg gcgtgctcat ggtcggcggg ttcctccagt tccgcgtgtt catggtcatc
    10801 tgcgggttca gtgtgttctg caagctcagc atgactgtgc cggtgcaacg ctagatccgg
    10861 gttgattctc gttcccgcta cgatgccgag catcgctgta gtcgatatca gaagggcgac
    10921 gactgtggcg attgctgccg cggcaaatgg ccgcctaaac actcgccggc aagatctcca
    10981 aagcaaccat ccaaggcaga cctcaagccc tcctataagg gcatttagcc ctacgacggt
    11041 gatgccgccg tgcgcggtga gcgccaggat caagttgaca acgagtgcgg cgatgaaccc
    11101 ctcggcgggc ccggcgatca tgcctgtgag gactgtgagg ttcaggtggt agcccagcac
    11161 cggtatggga agacccatcg ccagcagcat gagcgcggag agagtggcga ttcgcgggac
    11221 gaccctggcg cggtctcgca tgctgagagc ccgaagcgca acacagacta gaagcaccgt
    11281 taagccaatc cccgccagga tccagggcag ggggatcaac ccgtctggga tgtgaagatg
    11341 cgacatgtat cagttgtcct cctttgcgcg ttcgcgacac ctagggcact ggccgatcag
    11401 ttccagccaa tgtccttcaa tacagaaacc gctcattctc tccagttcgt gctctatcga
    11461 tctgatcaga cagtcgccga attcgactac ctgaccgcat ccggtgcaca ccaaatggtg
    11521 atgatgaccc gagggatccg ggcgtcgctc gtaccggtcg aaaccgtcgt tgaacttgat
    11581 cttgtcgagg attcctacac ttgtgaacag ctccaggcag cgatacacgg ttaccaggcc
    11641 aatgccgggt tcccgtcctc ggatcgcgtc gtgagcctgt tgagcagtga gatgctgagc
    11701 gccgtcggag tagaaaagct ccagcaccgc tctgcgctgc ggcgtcagct tgaacccgcg
    11761 ttcacgaagt ttgtggagag cgtcatcgaa gcagtcggcc tgcacctgag cccctcccag
    11821 ccgtaaatga aaaccacttt caattactat gcccattata gcccctccag actccccggc
    11881 gcaatatggg cgccgtttga catatgggcg ctgcttgacg aagaacttgt ggtgcgtcaa
    11941 gtctaggagg agaattgggg gcgagtttca aacgatgtac atcttgttca caaagtgaac
    12001 acctctgggg gtcggtccag tcaggcatca gggtcgcggg tgcggcggta tacccccaag
    12061 ggtatcttgg caggttttct cccctccgga ggcgttagac ggagattttc tgcacacatg
    12121 cggcgaaaaa tctccgcgtg agcgcgcccg gactactgat tctgccaaga ccatggcaat
    12181 aagcggcacg ctgaagcagg caaggcgttc aggggtgctc atatatggga tttctgtatg
    12241 ccgcaggatg tcggtggctg ttattagtat tgcgatggta cctgatcttc tgtgttatgg
    12301 cgcccacgcg gtccgaaacc agcgtcgcgg agattttctg cacacatgcg gcgaaaaatc
    12361 tccgtgttga gccgcggaga gtgcaaatat ccgcaggccc agccaaaagg cgcccgcacg
    12421 ccgacgactc cgcgagcaag cggccttcgc agtcgccggc tccgggaggc gcgatcatcg
    12481 tggacacctt gcgcttgttc gattatgccc caactatcgc ccgttctggg agcccatcgc
    12541 cgaaaacgtg ttccgtaagg cggcacccag atttcgctac gactacgcgg accgccgcta
    12601 cgacttttcc gccgccaagc ttcgcttcgt atacagccta gactgtgcgc ccatggggat
    12661 gggttcatta acgggttggt tggctatggt cgcgctcaca cggagactcc tcgctgcaca
    12721 cgtgcaggca gtctccgtcc accgcctcca tggggaggag gttgctccga tatccttgag
    12781 ggcggtacac ggcagcgcct gtggccgtga tgcctgactg gacggaagcc caaaggtgtt
    12841 tctttgtgaa caagatgggc atcatctcca gcgatgcggg taacgcatag tgccctatgg
    12901 gccaggattt ctggggggcc taggtagatc gacataggat ggccgaaaaa cctggcccgt
    12961 agggcatagc ggtcccgaac ggtaccatga ctcctgtagt tcatttggta gaggcggggg
    13021 ccataatatg gcaacgttgg cgtatctccc gagttcctcc ggaaaggccc ttaggcaacc
    13081 gataattcgc tgagaaaacc caagtcagcc cggttttctc cccgtgaccg tcaaaaccgc
    13141 gcacttgctg tgcagttggc gtacagaccg cactgtcttg ttacagggtc gctaactccc
    13201 tgctaaaccc gttgttactg aacgggctgc ccaatagata gatttagctg gccttttgat
    13261 atgcagttgc gtcgtgaacg ggtttccgca gaggtgtcgc gaattcgagt gacggttggt
    13321 gcataggcgc cgttgtagcc caccgcggga atgtgaccgc agtaatggcg ggaaccgcat
    13381 gacgcgacag gagaacaggt gaggaggatt tgccgcgact acggagaaag gctaacccag
    13441 cgcagcgcgg gttgaagcgg gcagggcaga ggtctggcgg agaccgggcg cagccggagc
    13501 gcaggttggc ggcaggctag gggtaggttg ggggcacacc gccggctgct gtatcgaggg
    13561 agtgaggtat catggattct gggcagggta tgcgtgtcgt ggagtgtgtg cccaactttt
    13621 cggagggccg gaggccggaa gttgtagcag aaatcgtgtc ggccatcacg tcagttgaag
    13681 atgtgaagtt gctcgacttt tcgtccgacg caagtcataa ccgttctgtc gttacctttg
    13741 tcggaacgcc ggaggtcgtg gaggcggcgg cgtttgcagg ca
//
LOCUS       DAOTAC010000025        13283 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_3924_length_13283_cov_4.960765, whole genome shotgun sequence.
ACCESSION   DAOTAC010000025 DAOTAC010000000
VERSION     DAOTAC010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 13283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13283
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3924_length_13283_cov_4.960765"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="PLM74_02015"
     CDS             <1..426
                     /locus_tag="PLM74_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HON41891.1"
                     /translation="MRDIQRVFAYHGAEHKTIAAWEAGEELTVENARRHGTAHARCGT
                     NFLLIVMLVSVLIYSLLGKQALWERIVTRILLLPVLAGVSYELLKIAGRPDPSPIVKW
                     ARAPGLALQRLTTREPDDSMLEVAIVSLQAVIEKDEAVN"
     gene            429..1496
                     /gene="prfA"
                     /locus_tag="PLM74_02020"
     CDS             429..1496
                     /gene="prfA"
                     /locus_tag="PLM74_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HON41892.1"
                     /translation="MFEKLKEIETRYEELEREMAEPGVIADQLRYKKVTKAHSDLEDI
                     VAHYRELKKVMGEIQATGEMLRDKLDSELRELAEAELASLEEDKSSLEEKLKVLLLPS
                     DPLDEKNVIMEIRAGTGGDEAALFAGDLFRMYSRYAERNGWQIESMSSSPTELGGFKE
                     VIVMISGDRVYSRLKYESGVHRVQRVPETEASGRIHTSAATVAVLPEAEEVDIEIDPN
                     DLRVDVFRSSGHGGQSVNTTDSAVRITHIPTGMVVTCQDEKSQHKNKDKAMKVLRARL
                     LDQLMEEQEAGIAETRKTQVGSGDRSERIRTYNFPQSRVTDHRIGLTTHRLTEFLDGD
                     LDEMIEALVVSEQSERLKKAE"
     gene            1513..2430
                     /gene="prmC"
                     /locus_tag="PLM74_02025"
     CDS             1513..2430
                     /gene="prmC"
                     /locus_tag="PLM74_02025"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HON41893.1"
                     /translation="MNTAATTSQAPQLIDILRRSTAFLARNGVESARLDAEVLLADVL
                     EMERIQLYVQHDRPLIHDELERYRDALKRRARRMPVAYVTGRKEFMSLDFAVDSRVLI
                     PRPDTEVLVEAVCSRLAALGIDDPIVVDIGTGSGAIACAIARGVPGARVLATDISGGA
                     LEVAASNVAGLGLTGRVLFAQGDLFEPTSGTEFEHMDVLVSNPPYVPSSDIPSLAPEI
                     SRYEPRLALDGGPDGLDFYRRIADGAPSRLTGRGFVALEVGEGQAASVSDILEQAGLA
                     DLEAICDYSGAIRVVVGSAPGVRGDTDVL"
     gene            2420..3565
                     /locus_tag="PLM74_02030"
     CDS             2420..3565
                     /locus_tag="PLM74_02030"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HON41894.1"
                     /translation="MCCDSSRNGATPVNTVRMAVDPKSPSQDLEAQLSSAAAILRSGG
                     LVAFPTETVYGLGANARDGRAVARIFQAKGRPQDNPLIVHIACLADLRAVVADITDDA
                     WALARAFWPGPLTIILPVGGDIAREVTAGGDTVAVRMPSHPVAMALIRASGLPVAAPS
                     ANISGRPSPTRADHVWADLAGRIEMIVDGGPAGIGVESTVLDITSSPPMVLRPGGVSV
                     EELRRIVPGVCVLPQYGTGAADGDEQEGPARSPGLRHEHYSPRAQLWLFTGEWEAQVQ
                     AIACRARAEAEAGKQVGLLISAETAQMVARADIPNTAVIAEVGSRFCLASVASRLFAA
                     MRQLDSEGVQIILAESYDTAGVGLAVMNRLVRSAGGRLVGPGESGRK"
     gene            3606..4034
                     /locus_tag="PLM74_02035"
     CDS             3606..4034
                     /locus_tag="PLM74_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HON41895.1"
                     /translation="MPRRILLVCSGNTCRSAMAEAIVRNLIESGSYPEDWTVESAGIS
                     AVDGDPATDNAVAVLAELGLDLAGHRARTLTPEMVSQADTILAMERRHLQRILELDPL
                     AAPRACLLAGSDIADPFGGGVEVYRYARDEIRSSIAARLG"
     gene            4058..4510
                     /gene="rpiB"
                     /locus_tag="PLM74_02040"
     CDS             4058..4510
                     /gene="rpiB"
                     /locus_tag="PLM74_02040"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HON41896.1"
                     /translation="MRIAVGSDHAGFDAKQDVLRTLKEQGHEVRDMGAYSRESVDYPD
                     IAVAVSESVARGEADRGILVCGTGIGMSITANKVRGIRAALCHDEFTARAAREHNDAN
                     VLAVGARTISVHAILRIVDIFLTAQFAGGRHAARVQKIAECESKYGGC"
     gene            4504..5142
                     /gene="upp"
                     /locus_tag="PLM74_02045"
     CDS             4504..5142
                     /gene="upp"
                     /locus_tag="PLM74_02045"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HON41897.1"
                     /translation="MLATGKVHVIDHPLIQHKITLIRDKNTGPKDFRDLVGEVAMLMA
                     YEVTRDLVCETVEVETPIGQATGKQLAGKKVGVVAILRAGLGMVDAVLKLIPAAKVGH
                     IGLYRDPDTLRPVEYYCKLPPDVSERELILLDPMLATGGSACAAIQFAKDRGANSIKL
                     VNLIAAPEGIRLVQQTHPDVDIYVAAVDQCLNSHGYIVPGLGDAGDRLFGTK"
     gene            5172..5624
                     /locus_tag="PLM74_02050"
     CDS             5172..5624
                     /locus_tag="PLM74_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HON41898.1"
                     /translation="MSQSQRPPWDAYFLEIARVVASRSTCLRRQVGAVAVRDRQILAT
                     GYNGAPSGLRHCGEVGCIRESMQVPSGERHELCRGLHAEQNVIIQAALHGVALSRATV
                     YCTHHPCVVCSKMMINAGVTRVVYSGGYPDRLSCEMLAEAGVQLVQAP"
     gene            5642..6667
                     /locus_tag="PLM74_02055"
     CDS             5642..6667
                     /locus_tag="PLM74_02055"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012939058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HON41899.1"
                     /translation="MTRYLGALLLSTACSWLLTPEMRKIALARGIVDHPRGRHIHAQP
                     VPFLGGVAMYAAFFIAAMTFGRPDRNTLGLLTGGAFTVALGIADDRAELRARHKLLGQ
                     IAAAVIVVLFGVKIEFVTNPFGGMIYLGVFSIPITVVWIVAFMNVVNFIDGLDGLAAG
                     VSAIGSFAMAAAALGNHQSRLAVLSLALAGSALGFLRYNFNPAKIFMGDAGAMFLGFT
                     IAGISAIGALKTPATLVLAVPVIVMGVPVLDTAFAIFRRVRRGVPVSSADRDHVHHRL
                     LAMGMTQRQAVLTLYAVSALLAATACGVVAVDPRLGIGGTVTAFCALIIVGGRSGVLK
                     SEIRRRD"
     gene            6687..7838
                     /gene="wecB"
                     /locus_tag="PLM74_02060"
     CDS             6687..7838
                     /gene="wecB"
                     /locus_tag="PLM74_02060"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007938945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HON41900.1"
                     /translation="MRHIKVLSVFGTRPDTIKLAPVVNELSKYPELIDSVTCGTAQHR
                     EMMDQVLGVFGITPDYDLGVMRDDQTLFDIAERGLRGLQEVLERERPDITLVHGDTST
                     AFVGALASFYCKVKVGHVEAGLRTNQKYDPFPEEMNRRLVGCLSDLHFAPTGEHLGNL
                     LRENFPRSNIYVTGNTVVDAVLAVSAARKGFADARLASIDFTGRRLVLVTAHRRENIG
                     EPLEAICGAVRDVLGEFPDTLVVMPVHPNPRVRSTVVRELSGVDRCYLIDPVSYPDMV
                     GLMQRCFMVMTDSGGLQEEAPALGKPVLVLRRTTERPEGLRAGTLRLVGVSREGIRTE
                     AGILLSNTEKYRQMAESPNPYGDGKASKRIVSGILHHFGMVDERPADFA"
     gene            7954..9231
                     /gene="murA"
                     /locus_tag="PLM74_02065"
     CDS             7954..9231
                     /gene="murA"
                     /locus_tag="PLM74_02065"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HON41901.1"
                     /translation="MEALIIRGGRPLNGEVRVGGAKNASLPIIAASLLAPGSSSIEDV
                     PRLKDIDTMCGVLSSLGARVSRSPKIIAVDASDISSVEPPYELVRAMRASFLVMGPLL
                     ARMGRVRVSLPGGCAIGSRPIDLHLKGLAALGAEIDMGYGQIEARAARLTGARVYLDF
                     PSVGATENIMMAAVLASGMTLIENAAQEPEVVDLANFLNSMGAHVRGAGTNVLRIEGV
                     AELEPACHMVIPDRIEAGTLMVAAAMTRGSLAIDNVVDEHLKPITAKLREAGVSVEER
                     DDGSIYVEMRQRPTGIDIKTMPYPGFPTDLQAQFMALTSISSGSSVITETVFENRFMH
                     VSELARMGASIKIDGRSAMIEGQPGLSAAQVRCTDLRAGAALVLAGLVASGETVVSDV
                     YHVDRGYERLEEKLARAGADIRRLTESEVEKAV"
     gene            9368..10339
                     /gene="spoIID"
                     /locus_tag="PLM74_02070"
     CDS             9368..10339
                     /gene="spoIID"
                     /locus_tag="PLM74_02070"
                     /inference="COORDINATES: protein motif:HMM:TIGR02870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HON41902.1"
                     /translation="MARTASILVALCVGVMLLIPASIALLVRPLSLVRPTDIWISVWD
                     HSARVVRRLSLQDYLVGVVAAEMPASFELEALKAQAVAARTYTLRRLANDAARMANEH
                     PSGAQICTDPSHCQAWNSREELVGKWGKAAYLPNIRKVIAAVESTYGLVMTYNGQLID
                     AVYHSCCGGMTEDAAEVWGRRIPYLVPVACGCGRRGVELAETRAVDNAHLLAALGIGA
                     RGVPAMASGVRVAARTGTDRASIVSVYGVPVSAAQLRKTLSLKSTRIVISSEGGKTIF
                     RTTGYGHGVGMCQWGAASMAQRGDTFDVILRHYYTGVRIQQIASGEK"
     gene            10355..11011
                     /locus_tag="PLM74_02075"
     CDS             10355..11011
                     /locus_tag="PLM74_02075"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HON41903.1"
                     /translation="MRLSFLKNYRRTLPIVWAVCFALGFVVGAWIGPGITSTAGRRAT
                     TGQASPAQTVSSADITSAQGQPDDDSRAQDSQAMEALDVPAREPETMIMPAKGKTVGA
                     YGWYRDPVTDDWRFRDGLDISCAPGTSVAAALSGTVTGVSRAQGGGYSVSLKNAGDVN
                     TTYSNLRAVWVDVGQQVAAGKYLGDIGAASSASGGVLRFTVECDGQSLDPAVCLGQRF
                     "
     gene            11079..11336
                     /gene="spoIIID"
                     /locus_tag="PLM74_02080"
     CDS             11079..11336
                     /gene="spoIIID"
                     /locus_tag="PLM74_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HON41904.1"
                     /translation="MREYIRRRVLEITDHMLETRATVRQTARLFNVSKSTVHKDVTER
                     LPSISKQLARKVKKILDQNKAERHIRGGEATRRKYRASGRA"
     gene            11397..12410
                     /locus_tag="PLM74_02085"
     CDS             11397..12410
                     /locus_tag="PLM74_02085"
                     /inference="COORDINATES: protein motif:HMM:NF010539.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HON41905.1"
                     /translation="MAWSFADVGVDLGTERVRVAVRGRGVIIDEPSAVAVNRQTGLFV
                     AAGHQAEELLADDPYKLVRLRPLSNGAVGRYDCALMLLRSVMSRAVGGRGLARPRVAM
                     SVPARPSQVEKRTWLQVAIESGARATALVEAPMAAAIGAGVDVLEPSGAMVVDVGAQV
                     TDMGVISTGGIVVSDRLKIAGTSMDRAIQVHARDVHGLVLGQRSAEKLKIELGAEAWT
                     GQEASAVAHGRNLADGLPRAEAFCAREISSALERPINQIIESARCVFGCTPPELAADI
                     AAGGIILTGGGSRLAGLSGLMSERMGVAFRVALDPDTCVIRGIGELLHGMDPRVTCAW
                     KGV"
     gene            12464..>13283
                     /locus_tag="PLM74_02090"
     CDS             12464..>13283
                     /locus_tag="PLM74_02090"
                     /inference="COORDINATES: protein motif:HMM:NF017400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HON41906.1"
                     /translation="MHKRLVDRTAATAATAMIAAVAIVMLCGSMFWGPSVYAAQERVD
                     TIDVSELRPGMKGHGMTVFRGLEPESFDVEVIGIIRRGGQLGNMVLVRVSGAAVEAAG
                     GVAEGMSGSPVYVGGKLAGAIAYVFPGSDHFVAGVTPIADMLRMLDYPDALGAFEEVP
                     AGARAATSLVVSGLSGRALGRLSEALERFGATVRPAASFGLGAGAGASEWVGRTGSTG
                     SSESVSGGRPAQEIKPGSAIALQLAQGDVEITAFGTVTYVEGDKFLAFGHPVLGN"
BASE COUNT         2596 a         3590 c         4415 g         2682 t
ORIGIN      
        1 atgcgcgata ttcagcgcgt atttgcgtat cacggcgcgg agcacaagac gattgctgcg
       61 tgggaggccg gcgaagagct cactgtggaa aacgcccggc gccacggcac ggcccatgcg
      121 cgctgcggca cgaacttcct gctcatagtg atgttggtga gcgtactcat ctactctctt
      181 ctgggcaagc aagctctatg ggaacgcatt gtgacccgta tcttgctgct gccggttctt
      241 gccggagtgt catatgaact actcaagatc gcggggcggc ctgatccatc gcccatagtg
      301 aaatgggcgc gggcgcccgg tcttgcgctg cagcgactta cgactcgcga acccgatgac
      361 tccatgctgg aagtggctat cgtgtcgctc caggcagtca tcgagaaaga tgaagcggtg
      421 aactaaccat gttcgagaag cttaaggaaa tcgaaactcg atacgaggag ctggagcggg
      481 aaatggccga gcctggggtc atagccgacc agctgagata caagaaggta accaaggccc
      541 attcagatct cgaggatatt gtcgcgcact atcgcgagct caagaaggtt atgggcgaga
      601 ttcaggcgac gggggagatg ctccgcgaca agctcgactc ggaactacgt gagcttgccg
      661 aagcagaact ggcgtctctt gaggaggaca aatcatcgct tgaggagaag ctcaaggtgc
      721 tcctgttgcc gtccgacccc ctagacgaga aaaacgtcat aatggagatc agggccggca
      781 caggcggcga tgaagcggct ctgtttgcgg gagacctctt ccggatgtac tcgcgctatg
      841 ccgagcgcaa tggctggcag attgagagca tgagctccag cccaactgag cttggtgggt
      901 tcaaggaagt catcgtcatg atctcaggcg accgagttta cagtcgcctg aagtacgaaa
      961 gcggagttca ccgtgtccag cgtgtgcccg aaacggaggc ttccggaagg atacacacgt
     1021 ctgccgccac tgttgctgtg ctgcctgaag cggaggaggt cgacatcgag atcgacccca
     1081 atgacctccg ggtggacgtg ttcaggtcgt ccggccacgg cgggcagagc gtgaatacga
     1141 ccgactccgc cgtgaggatc actcacatcc ccactgggat ggttgtgacc tgtcaggacg
     1201 agaaatcgca gcacaagaat aaggataagg ccatgaaagt tctgagggcc cgtcttctgg
     1261 atcaactcat ggaggagcag gaagcaggca tcgccgagac ccgcaagacg caggtgggct
     1321 cgggggatcg gagcgagcgt atacgcacgt acaacttccc ccagagcagg gtgacggatc
     1381 atagaattgg gctcactact catcggctga ccgagtttct cgatggcgac ctagacgaga
     1441 tgattgaggc gctcgttgtg agcgaacaga gtgagaggct gaagaaagcg gaataggggc
     1501 ggtttggggg caatgaacac ggcagcgact acttctcaag cgccgcagct gatcgatatt
     1561 cttcggcgct ccaccgcttt tctggcaagg aacggagtcg agtccgcaag gcttgatgcc
     1621 gaggtcttgc tggccgacgt gctcgaaatg gagcgtatcc agctgtacgt tcagcatgac
     1681 cgtccgctca tccacgatga gttggagcga tacagggatg ctcttaagcg tcgcgcacgg
     1741 cggatgccag tggcgtatgt taccgggcgc aaggaattca tgtcgctcga tttcgctgta
     1801 gacagccgcg tgctgatacc gcgccccgac actgaagtgc tggtcgaggc ggtgtgcagc
     1861 cggctggctg ctttgggcat cgatgatccg atagtagtag acatcggtac cggcagcggc
     1921 gcgatagcgt gtgccatagc gcggggagtt cccggagcgc gggtgctcgc aacggacatt
     1981 tccggtggtg ctctggaggt ggcagcatcc aatgtggcgg gattggggct cacaggtcgc
     2041 gtcctctttg cgcagggaga cctgtttgag cctacatccg gcacagagtt cgagcatatg
     2101 gatgtgctgg tgtccaaccc tccatacgtg ccgtcgagcg atattccttc tctagcccct
     2161 gagatatcgc ggtatgagcc acgcttggca ctggacggag ggccggacgg cctggacttc
     2221 taccgtcgca ttgctgatgg cgcgccaagc cgcctcacag ggcgtgggtt cgttgcgcta
     2281 gaagtaggtg agggccaggc ggcttcggtg tctgatatcc tggagcaggc ggggttggcc
     2341 gatctcgagg cgatttgcga ctactcgggc gcgattcgcg ttgttgtcgg ctcggcgccg
     2401 ggagtcaggg gtgacaccga tgtgctgtga cagttctcgc aatggcgcaa ccccggtcaa
     2461 cactgtgcgc atggcagtcg atccaaagag cccgtctcag gatctcgagg cgcagctgtc
     2521 gtcagcagct gccatcttgc gatcgggcgg gctcgttgca tttcccacag agactgtgta
     2581 tgggctaggc gccaatgccc gcgacggacg ggccgttgct cgcatcttcc aggccaaagg
     2641 caggccgcag gacaatccgc taatcgtcca catagcgtgt cttgccgact tgcgcgccgt
     2701 cgtcgccgac attactgatg acgcctgggc tcttgcgcgt gctttctggc caggtcccct
     2761 caccattatc ctccccgtgg gtggtgacat tgcccgagag gtcactgcgg ggggcgacac
     2821 agttgcggtt cgaatgccgt ctcacccggt ggccatggcg ctgatccgcg cttcagggct
     2881 tccagtcgct gcgccgtcgg ccaacatatc cgggcgcccc agccctacga gggctgatca
     2941 tgtgtgggca gatcttgcag gcaggattga gatgattgta gatggcggac cggccggtat
     3001 cggggtagag tcgactgtgc tagacatcac atcgtcgcct ccgatggtgc ttcggccggg
     3061 tggcgtgtcg gtggaggaac tccgccgcat cgtgcctggt gtgtgcgtgt tgccccagta
     3121 tggcacgggc gctgccgatg gagacgaaca ggaagggccg gcgcgctcgc ccgggttgcg
     3181 ccacgagcat tattctcccc gggcccagct gtggctgttc acaggagaat gggaggctca
     3241 ggtgcaggcg atcgcctgca gggcccgggc tgaggcagag gcgggcaagc aggtagggtt
     3301 gctgatcagt gctgagacag cccagatggt ggcgagagcc gacatcccaa acacggccgt
     3361 cattgcagag gtagggtcca gattctgcct ggcatcggtg gcgtcaaggc tgtttgccgc
     3421 tatgaggcaa ctggatagcg agggcgtcca gatcatactg gccgaatcct acgatactgc
     3481 cggggttggc ctggccgtaa tgaataggct ggtgagatcg gcgggaggaa ggcttgtcgg
     3541 cccgggcgaa tcaggcagga aatgagcttt tttcagcgaa attcatcgca cggacggtga
     3601 atgagatgcc gcgacggatc ctcctggtgt gtagcggaaa cacctgcaga agcgcgatgg
     3661 ctgaggccat agtcaggaac ctcatcgaat ccggttcgta ccccgaggac tggacggtcg
     3721 aatccgcggg catcagcgcc gtggacggcg atccggcaac tgataatgct gtagccgtcc
     3781 tggccgagct ggggctggat cttgcaggcc accgtgcgcg cacacttacg cccgagatgg
     3841 tctcgcaggc cgacactatt ctggcaatgg aacgcaggca tctccagcgc attctggagc
     3901 tcgatccgct ggccgcgccc cgcgcgtgcc ttcttgccgg gtccgacatc gccgatccgt
     3961 tcggcggcgg ggttgaggtc taccggtacg ccagagatga gatacgaagt tctatcgcag
     4021 ccagactggg atgatggaga gcggaggtgg tgactgattg agaatcgctg tcgggtccga
     4081 ccacgcaggt ttcgacgcga aacaggacgt attgcggacg ctgaaggaac aggggcacga
     4141 agtccgcgac atgggtgcgt attcgcgcga gagtgttgac taccccgata tcgctgtggc
     4201 cgtgtcagaa agtgtcgcgc gaggcgaagc cgatcgcggc attcttgtgt gcggcaccgg
     4261 tatcggcatg tcgataacgg ccaacaaggt tcgcggcatc cgagctgcgc tgtgccacga
     4321 cgagttcact gcacgcgcgg cgcgcgaaca caacgacgcc aacgtcttgg cggtgggggc
     4381 gcgcactatc tccgtccatg caatcctgcg tattgtcgac atcttcctca ctgcccagtt
     4441 cgcggggggt cgccatgccg caagggttca gaagatcgca gagtgtgaaa gtaaatacgg
     4501 ggggtgttag ccacgggaaa ggttcacgta atagatcatc cgttgatcca gcacaagata
     4561 acgctgataa gagacaagaa caccggaccc aaggacttcc gcgatctcgt gggagaggta
     4621 gccatgctca tggcgtacga ggtgacgcgt gatctggttt gcgagactgt tgaagttgaa
     4681 acccctattg gacaggcgac cggcaagcag ctcgccggca agaaggtcgg agtcgtcgcc
     4741 attctccgag ccggcctggg catggtggat gcggtactga agctgattcc agctgccaag
     4801 gttgggcaca tcggcctgta ccgcgatccc gatacgctcc gtccagtgga atactattgc
     4861 aagcttccgc ccgatgtgtc agaacgagag ctgatcttgc tggatccgat gctcgccaca
     4921 ggcggatcgg cctgtgccgc aatacagttc gccaaggaca ggggcgcaaa cagcattaag
     4981 cttgtcaact tgatcgcggc gccggaaggc atacggttgg tgcagcagac ccatcccgac
     5041 gtggacatat acgtggcggc ggtcgatcaa tgtctgaact cgcacggata catcgtgcct
     5101 ggactgggag atgcaggcga ccggctgttc ggcaccaaat aggagaagac cgaactcgtg
     5161 aggtggagag tgtgagccag tcgcaacgtc ccccctggga cgcctatttc ctggagatcg
     5221 cgcgtgtagt ggcaagcagg tctacatgcc tccgccggca ggtgggggcg gttgccgtac
     5281 gtgataggca gattctggcg actggctaca atggcgcgcc ttccggcctc aggcattgtg
     5341 gcgaggtcgg gtgcattcgc gaatcgatgc aggtgccgtc gggtgaaagg cacgagctct
     5401 gtcgcggcct gcacgcggaa cagaatgtga ttatccaggc ggctctgcat ggggtagctc
     5461 tgtcgcgcgc tactgtgtac tgcacgcatc atccgtgcgt agtctgttcc aagatgatga
     5521 ttaacgctgg cgtaaccagg gtcgtgtact ccggcggcta cccggacagg ctgtcctgtg
     5581 agatgctcgc cgaggcaggc gtacaactgg tgcaggcgcc ttaacgtagg agtgcttcaa
     5641 cttgactagg tacctgggag cgctgttgct ctctactgca tgctcatggc tccttactcc
     5701 cgagatgagg aagatcgcgc tggcccgcgg tattgtggat catcctagag gccgacacat
     5761 ccacgcccag cccgttccgt ttctcggggg agtggccatg tacgcggcct tctttatcgc
     5821 tgcaatgaca ttcggacggc ccgatagaaa cacattgggg cttctaaccg gaggggcatt
     5881 cacggttgcg ctgggcatag ccgacgacag ggctgagctg agagcccggc acaagcttct
     5941 cggccagata gcagcggcag tgattgtggt tttgttcgga gtgaagatcg agttcgtcac
     6001 gaacccgttc ggaggcatga tatacctcgg cgttttctca atacccatca ctgttgtgtg
     6061 gatcgtggcc ttcatgaacg tcgtcaactt cattgacggg ctggacggcc tggcggccgg
     6121 ggtttcggcc atcggttcat tcgcaatggc ggcggcggcg ctcggaaatc accagtctag
     6181 gctggcagtg ctgtcgcttg ccctcgccgg cagcgccctt ggtttcttgc ggtataactt
     6241 caatcccgcc aagatattca tgggcgacgc cggcgccatg ttcctggggt tcacgatcgc
     6301 cgggatctcg gccataggcg cgctgaagac cccggccacg ctggttctgg cagtgccggt
     6361 catagtcatg ggagttcccg tgctcgacac cgcttttgcc atattccgcc gtgttcggcg
     6421 cggagtgcct gtatcgagcg ccgatcgcga ccacgtgcac cacaggctgc tggccatggg
     6481 gatgactcag cgtcaggcgg tgctcactct ctatgcagta agtgccctgc ttgcagccac
     6541 tgcgtgcggc gttgttgctg ttgatccgcg tcttggcata ggtggcacgg taactgcgtt
     6601 ctgtgccctg ataatcgttg ggggcaggtc gggggtgctc aagtcggaaa tacgccgccg
     6661 ggactaactt gcacggaggt ccatgaatgc gacatatcaa agtcctctca gtctttggaa
     6721 ctaggcccga cacaatcaaa ctggctcctg ttgtcaatga actatccaag tatcccgagt
     6781 tgatcgattc cgtgacctgc ggaactgcgc agcacagaga gatgatggat caagtgctcg
     6841 gcgttttcgg cataactccc gactacgatt taggagtcat gcgcgatgac cagacgcttt
     6901 tcgacatcgc cgagcgaggc cttcgcgggt tacaggaggt gcttgagcga gagcgcccag
     6961 acattacttt ggttcatggc gacacgtcta ccgcatttgt gggcgcgctg gcgtcgttct
     7021 actgcaaagt caaggtcggg catgtcgagg caggactgcg gaccaatcag aagtacgacc
     7081 cgtttccgga agagatgaac cgaaggctcg taggatgcct ttcggacctg catttcgctc
     7141 caactggtga gcacctcggc aacctgctgc gggagaattt cccccgatcg aacatatacg
     7201 tcacagggaa cactgtagtc gatgcggttc tggctgtttc cgctgcccga aagggctttg
     7261 ccgatgcgag gctcgcatct atcgatttca cgggaaggcg cctggttctg gtaactgctc
     7321 accggcgcga gaatatcgga gagcctttgg aggccatctg cggcgctgtg cgcgatgtgc
     7381 tcggcgagtt ccccgacact ttggtggtta tgcctgttca tcccaaccca cgggttcgaa
     7441 gcacggtagt tcgtgagcta tccggggttg acaggtgcta tctgatcgac ccggtaagct
     7501 accccgacat ggtcggcttg atgcagagat gctttatggt catgaccgat tccgggggcc
     7561 tgcaggagga agcccccgcg ctgggcaagc ctgtactggt gcttcgtcgt acgactgaac
     7621 gacctgaagg gttacgtgcg ggcaccctga gactggtggg cgtatccaga gaaggtatcc
     7681 gcacagaggc agggattctt ctgtcgaata cagaaaagta tcgccaaatg gcggaatccc
     7741 ctaaccctta tggagacggc aaggcatcta agcgaatcgt cagtggaata ttgcatcatt
     7801 tcggcatggt tgatgagcgc ccagctgatt ttgcctagcg gagcagcgga ttacatatct
     7861 tactagttgt gcgccgacgt gccgcggtgc tacactaccc gggggtattg ggccatcttt
     7921 caggcgattg gattgtgatt gggggcagtc ggcgtggagg ctctgatcat caggggcgga
     7981 aggcctttga atggggaagt gcgtgtgggg ggcgcgaaga acgcgtcgct tccgataatc
     8041 gccgcttcac tccttgcgcc cggatcatcg agcattgagg atgttccgcg tcttaaggac
     8101 atcgacacca tgtgcggggt gctatcgtcg ctgggcgcca gggtgtctcg ctcacctaag
     8161 atcatcgccg tagatgcctc cgacatatcc tcagtggaac ccccatatga actcgtcagg
     8221 gcaatgcgcg cctcgtttct ggtcatgggg ccgctgctcg cgcggatggg gcgagtgcgc
     8281 gtttctcttc ccggaggatg cgcaatcgga tcgcgtccca tcgatctgca ccttaagggg
     8341 ttggccgcgc ttggggccga gatcgacatg ggctacggtc agatagaggc aagagccgcc
     8401 cggctcaccg gagctcgggt ctatcttgac tttcccagcg tcggggcgac tgagaacatc
     8461 atgatggcgg cagtgctggc gtccggcatg acgctcatcg aaaatgctgc tcaggaacct
     8521 gaggtagtgg acttggccaa cttcctgaac tccatggggg cgcacgtaag gggagcgggc
     8581 actaatgtgc tgcgaataga gggtgtggct gagcttgagc ctgcgtgcca catggtgatt
     8641 cccgatcgga tcgaggccgg cacgctgatg gtggctgcgg ccatgacccg tggttcgctg
     8701 gccatcgaca acgtggtcga cgaacacctg aagccgatca cggcgaaact gcgggaagcc
     8761 ggcgtttcgg tggaagagcg cgacgatggc tcgatatatg tggaaatgcg tcaacgccct
     8821 accggcatcg atatcaagac catgccatac cccggattcc ccaccgacct tcaggcgcag
     8881 ttcatggcgc ttacatcgat cagctcaggt tcatcggtga taaccgaaac cgtctttgag
     8941 aaccgcttca tgcacgtgtc tgagctggcc aggatgggcg cttcgataaa gatcgacggc
     9001 cggagtgcga tgatagaggg tcaacccggc ctgtcggcgg ctcaggtgag atgcacagac
     9061 ctgcgggccg gcgctgcatt ggtgctggcc ggcctagtcg cctcgggcga gactgtcgtg
     9121 tcggacgtgt accatgtgga ccgcggctac gaaaggctcg aagagaagct ggcccgggca
     9181 ggggccgata ttcgccgctt gaccgaatcc gaggtcgaga aggcggtcta gcctatctca
     9241 gccgtcggat cctgccttcc attttgtccc ctctgcttca gcccattcgt ctcacattca
     9301 tctgttctag ctttcccggc cacgaataga ctgtacggct ttccgcctgg gcggaggagg
     9361 tacagctgtg gcaaggactg ctagcatact tgtcgccctg tgcgtgggcg taatgctcct
     9421 gattcccgcc tccatcgctc tgctcgtgcg tcctcttagt ctggtccggc ccaccgacat
     9481 atggatatcc gtatgggacc actctgcgcg tgtcgtacgg cgtctttctc ttcaggacta
     9541 ccttgtggga gtagtggctg cggagatgcc ggcatcgttc gagttggaag cgctgaaagc
     9601 ccaggccgtg gctgcgagaa cttacacgct gcgacggctc gcaaatgatg cggcacgcat
     9661 ggccaatgag catccgagcg gcgctcagat ctgcaccgat ccctctcact gccaggcgtg
     9721 gaattcccgc gaggagctag taggcaagtg gggcaaagcg gcgtatctgc cgaacatacg
     9781 aaaggtgatt gccgccgtgg aatccaccta cgggttagtg atgacataca atggccagct
     9841 catcgacgcc gtataccact cgtgttgcgg aggaatgacg gaagacgcgg cagaggtctg
     9901 ggggcgaagg ataccatatc tggtccctgt ggcttgcgga tgtgggcgcc ggggtgtgga
     9961 gctagccgag acccgcgcag tagataacgc tcatcttctc gcagcgctgg gcatcggagc
    10021 gcgcggtgtg cccgccatgg cgtcgggagt aagggtggcg gcgcgcacag gcaccgatcg
    10081 cgccagcatc gtgtccgtgt acggcgttcc ggtatctgca gcccagctac ggaagacttt
    10141 atctttgaag tctactcgca tcgtcatcag ctctgagggt ggcaagacga tctttcggac
    10201 taccgggtac gggcatggtg tggggatgtg ccagtggggc gccgcgagca tggcgcagag
    10261 gggcgatacg ttcgacgtga tcctgcgcca ctactacacg ggtgtaagaa tacagcagat
    10321 cgcctcaggg gaaaaatagc cctgaggtgg ttttatgaga ctttccttcc tcaagaacta
    10381 cagaaggact ctaccgattg tgtgggcggt ctgcttcgct ctgggcttcg tagtaggagc
    10441 gtggatcggc ccaggcatca caagtacggc gggcaggcgc gccacgaccg gccaggcttc
    10501 gccggcccag accgtgtcgt ctgcggatat cacgtcggca caggggcaac cggatgacga
    10561 ctctagggcc caggattcac aggcgatgga agctctcgac gtgccggcaa gagagccgga
    10621 gaccatgata atgcccgcca agggcaagac ggttggcgcg tacggctggt acagagatcc
    10681 ggtcacagat gactggcggt tcagagacgg gttggacatc tcatgcgctc ccggaactag
    10741 tgtagcggcg gcgctgtcgg ggactgtgac tggggtatcg cgtgcgcagg gcggcggata
    10801 ctctgtaagc ctgaagaatg caggggacgt gaacaccacg tactccaacc tgagagcggt
    10861 gtgggtcgat gtggggcagc aggtggcggc cgggaagtat cttggcgata tcggcgccgc
    10921 ctcgtctgcg tcaggaggtg tacttcggtt cacggtcgaa tgtgacggcc agtccctaga
    10981 cccggccgta tgcctgggac aacgcttcta gctctagcat aatccacccc tgtacgaata
    11041 tagatctagc aaacgacagg ggggaggccg gacgccagat gagggagtat atccgccggc
    11101 gcgtcctcga aataaccgac cacatgctgg agactagggc gactgtgcgt caaacggcgc
    11161 gcctgttcaa cgtgagcaag agtacagtcc ataaggacgt gactgagagg cttccctcga
    11221 tcagcaagca actggcgaga aaagtcaaga aaattctgga ccagaacaaa gcggagaggc
    11281 acatcagggg cggtgaggcc acgcggcgta agtaccgtgc gagcggcaga gcgtgaccat
    11341 ttgacaggtt gcgcgacttg gcgggcgcgc gtggcgcgtg gaaggtaggg tgagctatgg
    11401 cttggtcctt cgccgatgtc ggagtagatc taggcacaga gagagtacgg gttgcggtgc
    11461 gcggcagggg cgttatcatc gatgagccct ctgccgtcgc tgttaacagg cagaccggtc
    11521 tgtttgtcgc cgctggccac caggcagagg aactgctggc ggatgatccg tacaagttag
    11581 tgcggctcag gcccctgtcg aatggggccg tgggcaggta cgactgcgct ctgatgctcc
    11641 tgcgcagtgt gatgtcgcgg gctgtagggg gaagaggcct agccaggccc agagtggcta
    11701 tgtcggttcc ggctcggcct tcgcaggtgg agaagcgaac gtggttacag gttgccatag
    11761 agtcgggcgc gcgcgcgacc gccctcgttg aggcccccat ggcagcggca atcggcgccg
    11821 gagtcgatgt tttggagcca agcggtgcta tggtggtgga tgtaggggct caagtcacag
    11881 acatgggcgt gatatcgacc ggcgggattg tcgtgtcgga caggctcaag atcgccggaa
    11941 cgtcgatgga cagagcgata caggtgcacg cccgagacgt gcacgggtta gtgctgggtc
    12001 agcgttcagc tgagaagctc aagattgagc tgggggctga ggcatggacc gggcaggagg
    12061 catcagccgt tgcgcacggc cggaatcttg ccgacgggtt gcccagggcg gaggcattct
    12121 gcgcgcgaga gatatcgtcg gctcttgaac gcccgatcaa tcagataatc gaatctgccc
    12181 gatgcgtgtt tggatgcacc cctcccgagc tggctgcgga cattgccgcg ggcggcatta
    12241 ttctcactgg gggtgggtcg cgtttggccg ggctcagtgg actcatgagc gagaggatgg
    12301 gagttgcgtt cagggtcgcg cttgatccgg acacctgcgt gattcggggc atcggagagc
    12361 tgcttcatgg gatggatccc cgcgtgacat gcgcatggaa gggcgtatga tgtgtggtat
    12421 gatacagagc aggagccatg agagaggggc gttgcggtct acgatgcaca agagattggt
    12481 tgatagaact gcagcgactg cggcgactgc aatgattgct gcagtcgcaa ttgtcatgtt
    12541 gtgtgggtct atgttctggg gccctagcgt gtatgccgcc caagagaggg ttgacacgat
    12601 cgacgtgtcg gagctgaggc ctggcatgaa gggacatggt atgaccgtgt ttcgcggcct
    12661 ggagccggag agcttcgacg tcgaggtgat agggatcatc cgccgagggg gccagctggg
    12721 caacatggta ctagtgcgag tatccggcgc ggccgtcgag gcggcgggcg gagtcgccga
    12781 aggtatgagt ggcagtcccg tatatgtagg tggcaagctg gctggagcga tagcatacgt
    12841 gttccccgga agcgaccatt tcgttgccgg cgtgactcct attgccgata tgctcaggat
    12901 gttggactac cctgacgccc taggcgcctt tgaggaggtc ccggcgggcg cgcgcgcggc
    12961 cacttccttg gttgtaagcg gcctatccgg gcgggccctg gggcggttat ctgaggctct
    13021 tgagcggttt ggagcgactg tccggcccgc ggccagcttc ggcctggggg cgggggcagg
    13081 agcgtcggag tgggtaggac ggaccggatc gacaggctcg tctgagtcgg tgagcggtgg
    13141 tcgcccggcg caggagatca agccaggctc agccattgca ctccagcttg cgcagggcga
    13201 cgtagaaatc accgcttttg gcacagtgac ctatgtggaa ggcgacaagt tcctggcctt
    13261 tggccatccc gtgctgggaa acg
//
LOCUS       DAOTAC010000026        13252 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_3934_length_13252_cov_5.135940, whole genome shotgun sequence.
ACCESSION   DAOTAC010000026 DAOTAC010000000
VERSION     DAOTAC010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 13252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13252
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3934_length_13252_cov_5.135940"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /gene="guaA"
                     /locus_tag="PLM74_02095"
     CDS             <1..790
                     /gene="guaA"
                     /locus_tag="PLM74_02095"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HON41907.1"
                     /translation="KGEACEVQKVFQGHFHMNLVVVDARDRFLGRLAGVVDPEAKRKI
                     IGEEFIRVFEEEARRLGDVRYLVQGTIYPDVLESQSQGRGGAGVKSHHNVGGLPENLR
                     FELVEPLRHLFKEEVRRAGAALGLPQSVVGRQPFPGPGLAVRIVGEVTRERLEVLREA
                     DAIVRDEIEKLAAAGGVWQYFAILPTIRSVGIVVEEGSRARSYSHMIAVRAVTSEDAM
                     TAEWARLPHDVLDAISRRIVSEVPGVNRVVYDITSKPPSTIEWE"
     gene            800..1690
                     /locus_tag="PLM74_02100"
     CDS             800..1690
                     /locus_tag="PLM74_02100"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HON41908.1"
                     /translation="MRTRLSTSSRSLFADLSLVGICVVWGTSFAIVKSVVGDINPATF
                     IAVRFGIASIILGLIASPRLKRLDRRTWTAGLLLAVPLIAGFLTQTYGLKATTASKAG
                     FITGMHVVFTPLLEYTIGRRKVLPYQTAGVAVALVGLGLLTLEGFGRPSAGDLLVLAC
                     AFAFGLHIVMLGHYSPRHDGFLLSFTQMVWSAVLAAVISGRDMAQALSFGPEVVWSVT
                     YLGATGTALAYLVQTIAQKYTPPTRAALIMQSEPVFAALFAWLLIGERMAAKQFAGAA
                     LILLGILVCQIGDIARPQEV"
     gene            1690..3141
                     /locus_tag="PLM74_02105"
     CDS             1690..3141
                     /locus_tag="PLM74_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HON41909.1"
                     /translation="MVLRIVEMALDRARAAGASYADARSLQQADEGLTVRNGHVEQAR
                     SGMSYGLGVRVLVDGQWGYAAGSVVSEAEAARLAGLAVEIAKASSVVRKREVRLAPVP
                     PAVDTWESPCRIDPWSVPTSRKVDMLREACKAAQSGGSDVRVAMAYMRAIREVKNYAS
                     TEGARITQTSTITSAGVRAVAVGAGEMQIRSYPGTFDGNVRQAGYEFVEEMDLAANAK
                     RVGAEAQALLSAEQCPSDTMTLILGTNQLALQIHESCGHPTELDRVYGTEASFAGTSF
                     LTTEKLGSYRYGSKFVNLYADSQIPGGLGTFAYDDEGTPACHSYLVKDGILVGYLMSR
                     ETAHDLGTVSNGAMRASGWDRMPIIRMTNICLEPGNDTLESLIAGTDDGVFMDGIKSW
                     SIDDRRLNFQFGSEIGYRIRNGQLAEMVKNPTYTGITPEFWGSCDGIAGPEEWVLWGV
                     PNCGKGEPLQIMRVGHGTSPARFRNVKVGVGRW"
     gene            3135..4487
                     /locus_tag="PLM74_02110"
     CDS             3135..4487
                     /locus_tag="PLM74_02110"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HON41910.1"
                     /translation="MVGKDRALSVCETAISACGADAAEVVLMLETDSLTRFANSEIHQ
                     NTTVDDATLFVRAVLGTRAGWASTNQITHDAVQEAALRAVQMARVQPENPDSPGLADP
                     AKYEEADGYDERTAAFTPADRAQAVSRIIRQADVHGLNASGSLRTRVTEVAVANSRGV
                     RAWTRGTDASLITVVMTGFESDAGSGYAEAASRRVGDLDFDTLGATAVHKCLSTRRPQ
                     DVEPGEYVVVMEPSAVATAMQMLAYMGMNGISFVEGRSYASGRLGTKVTSEMVTIADD
                     WRDPDTSPLPFDFEGTPRQRVTLIEQGTASGMIYDRSAAARANTKSTGHAVPGGGMPG
                     GYPLHLVMNAGDSSIEEMVAGTERGIYITRFNYANPVHPVKTIFTGMTKDGTFLIEDG
                     RIGRPLRNLRFTDSILDGLFASITDVSRDTGLLPTGNGMSIYRAPAIRARLQVTGSTV
                     "
     gene            4512..5978
                     /gene="gltX"
                     /locus_tag="PLM74_02115"
     CDS             4512..5978
                     /gene="gltX"
                     /locus_tag="PLM74_02115"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HON41911.1"
                     /translation="MNAECGSTVRVRIAPSPTGNCHVGTARTALYNMLYARKYGGKFI
                     LRIDDTDLSRSTKESEEGVLEGLRWLGIKWDEGPDVGGPYGPYRQMERAYTYREVADR
                     LMAEGKAYRCYCTPEELEAERREALAVGKPPRYYGKCRNLAPEECAKLEAEGRKHVIR
                     LRAPDKILTFVDLLRGHQQKDMGERGDFVIVKSDGSPVYNFATVVDEHYMAMTHVFRA
                     VEHLTNTFDQLAVYEAMGWDPPQFGHLTLMLNPDRTKISKRAGAVYIGEFRDMGYLPE
                     ALVNFLALLGWNPGTEQEIFSMDELVEAFSVETLSRSDAVFDIKKLDWFNGVYIRSLT
                     PEDLAARVRPFMEKAGYATSDQAFLEKAVTLVTDRLGKLTEAPDMLGFLFVDRVDLAR
                     ESFAGKGKLTPGDIAGALVECERVLGGLDQWTHEDIEASLRALAESLGWKVGDLLMQV
                     RVAITGRRVTPPLFESLVLLGREKSMARIARATRALEE"
     gene            5983..8313
                     /gene="pcrA"
                     /locus_tag="PLM74_02120"
     CDS             5983..8313
                     /gene="pcrA"
                     /locus_tag="PLM74_02120"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016609533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HON41912.1"
                     /translation="MGADLLDGLNPPQKEAVVCTDGPLLILAGAGSGKTRVLTHRVAY
                     LISHAGVPPYRILAVTFTNKAAAEMKSRVAGLAGPAARWVWVSTFHSFCARILREDIE
                     ALGYKKSFTILDESDQTTVIKRCLKQLNVSGDMFRPQSVLAAIGAAKNELMDVEVFRE
                     SARDVRQRTIVQLYEAYQGVLARDNALDFDDLIMIAVRLFRERPDVLEKYRNRFTYIM
                     VVEYQDTNHAQYMLVKLLAARTRNLCVVGDDDQSIYEWRGADIRNILEFERDYPEARV
                     VKLEQNYRSTQTILDAACHVIANNRDRKSKSLWTAREPGAKIALYSADTEKDEARFIA
                     DELDRLIVAEGRCYGDFAVLYRMNAQSRAIEEVLVNRGIPYRIVGGLKFYERREIKDV
                     LAYLRVIENPEDSVSLERIINVPRRGIGDSTLSAARALAQQKGLPLLEGVKMATESTE
                     LTTRARAALADFLATMSRLWPLRGEKTVSQITRAVLDETGYLAELEREQTIEAEGRIE
                     NLKELITVTQEFESRHAAQGTGAEAGVGAGPEGAQLEVHASGPNDLAAFLEEVSLLTD
                     LDRADLSTDQVTLMTLHSAKGLEFPVVFMAGMEENVFPLARSAYDPAALEEERRLCYV
                     GITRAKDRLYLVHAFTRAIFGSSTANPVSRFVEEIPGHLLSDAFAERYRVYHGGRGGV
                     PGRAWLGDGAGTRPEAVRAEKVASGVERAWATRQVPGRPEFEAGDRIKHAKFGEGTVV
                     SVQQSAGEWLATIAFPGAGVKQFVLSLAPIEKVRSV"
     gene            8323..10374
                     /gene="ligA"
                     /locus_tag="PLM74_02125"
     CDS             8323..10374
                     /gene="ligA"
                     /locus_tag="PLM74_02125"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HON41913.1"
                     /translation="MELADALGRVRELRELIRYHNHRYYVLDDPEITDAEYDALMREL
                     AALEEAHPELADPDSPSRRVGGEVAASFEPVMHEIPMLSLDNVFSEELLRAFDARVRR
                     ATGLPSVEYVAEPKIDGLAISLTYRDGRLSVGATRGDGERGENVTANLRTIRSIPLIL
                     PDGAPESLTVRGEVYMRKPDFVGLNEEREERGEPVFANPRNAAAGSLRQQDPHVTASR
                     KLDAFFYYLPISEQLGANTHWEALERMRNLGFRVNDKITLCRDIEQVIAFIHDIAGMR
                     ESLPYEIDGAVVKVNRFDLQRDLGFTSRSPRWATAYKYPAQQAETTVRDIVVQVGRTG
                     VLTPTAEFEPVDVGGVTVSRASLHNQDYVRAKDIRVGDRVIIQRAGDVIPEVVRVLTE
                     RRNGLEREFAMPAICPECGSGVVREAGEAATRCVNLSCPARIREGLIHFASRDAMDID
                     GMGPSTVERLLAAGLVRNVADIYTLTREQLKGIERMGEKSAANLAAAIDNSKANPLYR
                     VIFALGIRHVGENVAKLLAGHFRSMDALACATHVDLLSVPTVGEAIADSVVTFFESEE
                     NRAAIGRLAEAGVRMADEAPTGSETPGVNRSEFQGKSFVFTGALKRFTRQRAEEIAEM
                     LGGRAASSVSRKTDYVVAGEDAGSKLARARELGVPVLSEQEFVDMLDRAGIGAAMQGG
                     E"
     gene            10376..10684
                     /gene="gatC"
                     /locus_tag="PLM74_02130"
     CDS             10376..10684
                     /gene="gatC"
                     /locus_tag="PLM74_02130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HON41914.1"
                     /translation="MSLSREETARLADLARLSLSDDELARIAHDLTEIIDYMEKLAGI
                     DRAHVRPMSHVLGTVNVCRDDRAEPSLSQEEALANAPDRQGEFFRVPRIIDAGEASEA
                     "
     gene            10714..12195
                     /gene="gatA"
                     /locus_tag="PLM74_02135"
     CDS             10714..12195
                     /gene="gatA"
                     /locus_tag="PLM74_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HON41915.1"
                     /translation="MTLHELRDMLAQGQVSAREITSDVLAQIDEVEASVGAYVTVDRE
                     GALSSAEAADATIAQARRDGDFGAVGPLAGIPVSLKDNVCTRGMRTTCCSKMLEGFVP
                     AYDATVTARLREAGAVIVGKANMDEFAMGSTTQTSVFHMTRNPWDLERVPGGSSGGCA
                     AAVAAGEAVAAIGTDTGGSIRQPASFCGVVGLKPTYGRVSRYGVVAFASSLDTIGPLT
                     RDVRDCAVMLNAIAGRDERDATSAPDAVPDYTECVERGVQGLRIGIPKEFFGPGMSEG
                     VRDAVERALRALEGAGADIVEVSLPLVEHALAVYYLVATAEASSNMARLDGIRYGYRP
                     EGRGGMDVADLMSLSRGQGFGMEVKRRIMLGTFVLSSGNFDAFYGRALRSRRLLAEDV
                     QRALAECDCLVSPTAPTVAFRFDEEPDDPLAMYLQDIYTTLANLAGVPAMSVPCGLAD
                     GMPVGLQIMGRMFDEATLIRVGRAVELVSGIGGARPAVGGCAK"
     gene            12192..>13252
                     /gene="gatB"
                     /locus_tag="PLM74_02140"
     CDS             12192..>13252
                     /gene="gatB"
                     /locus_tag="PLM74_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HON41916.1"
                     /translation="MTERDDYEVVIGIEVHVELATESKVFCGCKTAFGAAPNTQVCPV
                     CLGMPGALPMLNKRALEYTIRAGLALGCTIAGWTKFDRKNYFYPDLAKGYQVSQYDIP
                     IARDGYIDIEVDGQPKRIRVRRVHLEEETAKLVHAGDDIISANQSLLDFNRSGIGLIE
                     IVSDADMRSADEARAYLTKLRSILVAIGVSDCKMEEGSMRCEANVSVRPRGSKEYGTL
                     VELKNIASFRAVHRAIQYESARQIDLVRSGGRVTRETRHWDDVRGETAFMRSKEQAAD
                     YRYFPEPDLVPLEIAPEWVEEIKGALPELPDARAARYVAEFGLPGSDAALIAESRELA
                     SFFDGVCDVLAKAGAGAVDR"
BASE COUNT         2627 a         3541 c         4536 g         2548 t
ORIGIN      
        1 caagggcgaa gcctgcgagg tccagaaggt attccagggc cacttccata tgaacctagt
       61 ggtcgtggat gcgcgggacc gcttcctggg ccggctggcc ggcgtagtgg accccgaggc
      121 caagcggaag ataattggcg aggaattcat ccgggttttc gaagaggagg cccgccggct
      181 gggcgatgta cgttacttgg tgcaggggac gatctacccc gacgtgctcg aaagccagtc
      241 gcagggccgc ggcggggccg gggtgaagag ccaccacaat gtgggcgggc ttcccgagaa
      301 tctccgcttc gaactagtgg agccgctcag acatctgttc aaggaggagg tccgccgagc
      361 gggcgcggcg cttggccttc cccagtcggt agtaggtagg caaccgttcc caggccccgg
      421 gctcgcagtc aggatcgtgg gagaggtgac ccgcgagagg ctggaggttc tgcgcgaagc
      481 cgatgccata gtgcgggatg agatcgaaaa gctcgcggcg gccggcggtg tgtggcagta
      541 tttcgccatc cttcctacga tacgaagcgt tggcatagtg gtggaggaag gttctagagc
      601 gcggtcgtac tcccacatga ttgcggtgcg cgccgtgacg agtgaggatg cgatgacggc
      661 tgagtgggca cgccttcccc acgatgtgct ggatgcgatt tcgagaagga ttgtctcgga
      721 agtccccggc gttaacaggg tggtgtacga catcacgtcc aagcccccgt ctactatcga
      781 atgggagtag ggcacgaaat tgcgtactcg gctttcgaca tcgtccagat cactctttgc
      841 cgacctcagc ctggtcggca tatgcgtggt gtggggcaca agctttgcga ttgtcaagag
      901 cgtggtcgga gatatcaatc ccgccacgtt cattgcagtt cgattcggaa ttgcgtcgat
      961 tatcctgggg ttgatcgcca gcccacgcct gaagaggctt gatagacgca cgtggacggc
     1021 aggcctgttg ctggcggttc cgctcatagc cgggtttctc acgcagactt acggcctgaa
     1081 ggccacgact gcctctaagg ccggctttat cacagggatg cacgttgtgt tcactccgct
     1141 tctggagtat acgattgggc gcaggaaggt actgccgtat cagactgcgg gggtagctgt
     1201 agccttagtc ggactgggat tactcacact tgaaggtttc gggcgtccgt cagcgggcga
     1261 tctgctcgtc ctggcgtgtg cgttcgcctt tgggcttcac attgtcatgc tggggcacta
     1321 ctcgcccagg cacgacgggt tcctgctttc gttcactcag atggtatggt cggcggttct
     1381 cgccgctgtg atttccggac gcgacatggc ccaggcgttg tcgttcggcc ccgaggtggt
     1441 gtggtccgtc acatatcttg gcgccacggg caccgcccta gcctaccttg tccagacaat
     1501 tgcgcagaaa tacactccgc caacgagggc ggctctgata atgcagtcgg agccggtgtt
     1561 tgccgcgcta ttcgcgtggc ttttgattgg agagcgcatg gctgcaaagc agttcgccgg
     1621 cgcggcgttg atactcctcg gcatactcgt gtgccagata ggcgatattg cacgaccgca
     1681 ggaggtgtga tggtgttgag aatagtcgaa atggctcttg accgggcgcg tgccgccggg
     1741 gcatcctatg cggacgcacg atcgctgcag caggccgacg agggcctgac cgtgcgaaac
     1801 ggtcacgtgg agcaggcacg cagcggcatg tcatacgggc ttggcgtaag ggtgctggtg
     1861 gacggacaat ggggttacgc cgcaggtagt gtcgtatcgg aggcggaggc agcccgtctt
     1921 gccggcctcg ctgtggagat cgccaaagct agcagcgtgg ttcgcaagcg cgaggtgagg
     1981 ctggcgcctg tgcctccggc ggtcgacact tgggaatcgc cctgccggat cgatccctgg
     2041 tctgtgccga cgtcgcgcaa ggtggatatg ctgcgcgagg cctgcaaagc tgcacagtcc
     2101 ggaggaagcg atgtacgagt cgccatggcg tacatgcggg ccatccgcga ggtgaagaac
     2161 tatgcaagca ctgaaggggc gcggatcact cagacgtcta ccattacgtc ggcgggtgtg
     2221 cgtgcagtgg cggtgggagc aggagagatg cagatacggt cgtatcctgg aaccttcgac
     2281 ggcaacgtgc gccaggccgg gtacgagttt gtcgaggaga tggacctagc tgcaaatgcc
     2341 aaacgcgtcg gagcggaggc acaggcgctg ctctcggcgg agcagtgccc ttccgacaca
     2401 atgacattga tccttggcac gaaccagctc gccctgcaga tccacgaatc gtgcgggcac
     2461 cccaccgaac ttgaccgcgt ttacggaact gaggcatctt ttgcgggaac gagctttctc
     2521 accaccgaaa agctgggcag ctacagatat ggctcaaagt ttgtgaactt gtacgccgac
     2581 tcccaaatcc cgggcgggct gggtactttc gcatacgacg acgagggaac gcctgcatgc
     2641 catagttatc ttgtgaaaga cggcatactt gtgggctact tgatgtcgcg ggagaccgcg
     2701 catgacctcg gaacggtgag caatggtgcc atgcgcgcca gcggatggga caggatgccc
     2761 atcatccgca tgaccaacat ctgtcttgag ccgggtaacg acactttgga atcgctgatt
     2821 gccggaactg acgacggcgt cttcatggac ggaatcaaga gctggagcat agacgatagg
     2881 cgactcaact tccagtttgg ctcggagata ggctaccgaa ttcgcaacgg ccagctcgcc
     2941 gagatggtta agaaccccac ctacacaggg ataacccctg agttctgggg ctcgtgcgac
     3001 ggaatcgccg gccccgagga gtgggtactg tggggagtgc ccaattgcgg caagggcgaa
     3061 cctcttcaga tcatgcgggt gggccatggc acatcgcctg cgaggttcag aaacgtgaag
     3121 gtgggtgtgg gaagatggta ggaaaggatc gggcgctcag tgtgtgcgag accgcgattt
     3181 ccgcctgcgg cgccgatgcg gcagaagtgg tactcatgct ggagaccgac agcttgacga
     3241 ggttcgccaa ttccgaaatc catcagaaca cgactgtcga tgacgctacg cttttcgtcc
     3301 gcgcggtgct cggcacgaga gccggctggg cctcaaccaa tcagatcacc cacgatgccg
     3361 tacaggaagc tgcgctgcga gctgtgcaga tggcacgcgt tcagccggag aaccccgatt
     3421 cgccgggcct ggccgatcct gcgaaatacg aagaggccga cgggtatgac gagcgaactg
     3481 cagctttcac cccagctgac agagcgcagg ccgtgagccg cataatacga caggcggacg
     3541 tgcatgggct caatgcttcc ggatcgctga ggacgcgagt gacggaagta gcggtggcta
     3601 actccagagg cgtcagggcg tggacccgtg ggacggatgc ctcattgata acagtggtaa
     3661 tgacggggtt cgagtccgat gccgggtcag gctacgccga ggcggcttct cggcgcgtgg
     3721 gcgaccttga cttcgacacc ctgggcgcta cggctgtcca taagtgcctt tccacgcggc
     3781 gccctcagga tgtggaaccg ggcgagtacg ttgtggttat ggagccttcg gctgttgcca
     3841 cagccatgca gatgcttgcg tacatgggca tgaacgggat atctttcgta gaaggcagga
     3901 gctacgccag cgggcgactg gggacgaaag ttacgtcgga gatggtcacg atcgccgacg
     3961 actggcgcga ccccgatacc tcacccctgc cattcgattt cgagggtact cccaggcaga
     4021 gggtgaccct gatcgagcag gggacggcct ctggaatgat ctatgatagg agcgcggccg
     4081 ccagggctaa cacgaagtct acggggcatg ctgttcccgg aggcggaatg ccgggcggct
     4141 atcctttgca tcttgtgatg aacgccggcg attccagcat tgaagagatg gttgccggca
     4201 cagagcgggg gatatacatc acgcggttta actacgccaa tccggtccac ccggtgaaga
     4261 ccatcttcac cggtatgacc aaagacggca cgttcctaat tgaggatggc agaatcggtc
     4321 gtcctttgag gaatctgaga ttcaccgact ccattctgga cgggctgttt gcaagcataa
     4381 cggatgtttc gcgtgatacg gggctgttgc ccacgggcaa cggcatgtcc atataccgcg
     4441 cgccggccat ccgggcgcgg ctgcaagtta caggatcaac ggtctaggca aagaggagct
     4501 gacaatggat catgaatgcg gaatgcggtt cgacggttag ggttcggata gcgccttcgc
     4561 ccacaggcaa ttgccatgtg ggcaccgcgc gcactgcgct ctataacatg ctttacgcca
     4621 ggaaatacgg cggtaagttc atcctcagaa tcgacgacac cgatctcagc cgcagcacaa
     4681 aggagtcgga agagggtgtg ctcgaaggac tgagatggct cgggataaag tgggacgaag
     4741 gcccggatgt cggagggccc tacggaccct atcgccagat ggagcgcgca tacacgtatc
     4801 gcgaggtcgc agataggctg atggcagagg gcaaggctta ccgatgctac tgcacacccg
     4861 aggaactgga agctgagcgt agagaggcgt tggcggtcgg caagccccca cgctactacg
     4921 gcaagtgcag gaaccttgcg cctgaagagt gcgccaagct cgaggcggag ggcagaaagc
     4981 atgtcatcag gctgcgagcg cccgataaga tcctgacctt cgtcgacctg ctccgcgggc
     5041 atcaacagaa ggacatgggc gagcgcggcg atttcgtgat agtcaagtcc gacggcagcc
     5101 cggtctacaa cttcgccaca gtcgtcgatg agcactacat ggctatgact cacgtgttcc
     5161 gcgctgtgga gcacctcacc aacaccttcg accagctggc cgtgtatgag gcaatggggt
     5221 gggatccacc ccagttcggg cacttgacgc tcatgctcaa ccccgaccgc accaagattt
     5281 cgaagcgcgc gggggccgtg tacatcggcg agtttcgcga catggggtac cttccggaag
     5341 ctctggtgaa ctttcttgcg cttctgggct ggaaccccgg cactgaacag gagatcttca
     5401 gcatggatga gctggtcgag gctttttccg tggagacgct ttcgcggtcg gacgccgtgt
     5461 ttgacatcaa gaaactcgac tggtttaacg gggtgtacat ccggagcttg acgcctgagg
     5521 acctggccgc cagggtgcgc ccgttcatgg agaaggccgg gtacgccact tcagaccagg
     5581 cgttcctgga gaaggccgtc acgttggtca ccgacaggct gggcaagctg accgaggccc
     5641 cggacatgct cgggttcctc ttcgttgaca gggtcgatct ggccagggaa tcgtttgccg
     5701 ggaagggcaa gctcacacct ggcgacatcg ctggagccct tgtggagtgc gagcgcgttc
     5761 ttggcgggct ggatcagtgg acccacgagg atattgaggc atcactgcgc gccttggcgg
     5821 agtcgcttgg ttggaaggta ggcgatctgc tcatgcaggt gcgtgtggcc attaccggcc
     5881 ggcgggttac cccgcctcta tttgaaagcc ttgttctgct ggggcgggaa aagtctatgg
     5941 ctcgcattgc ccgggccact cgggccctgg aggaataggc gcatgggcgc tgatcttctt
     6001 gacgggttga acccaccgca aaaagaggcc gtcgtgtgca ccgacggccc ccttctcata
     6061 cttgcaggtg ccggaagcgg caagacgagg gtgctcaccc accgtgttgc ctacttaatc
     6121 tcccatgcag gtgtaccacc ctacaggatt ctggccgtga cgttcaccaa caaggctgcg
     6181 gccgagatga aaagccgggt ggcggggctt gcggggccgg cggcgcggtg ggtatgggtg
     6241 tcgacattcc actcgttctg cgcgcgcata ctgcgcgaag atatcgaggc gctgggctac
     6301 aagaagagct tcaccattct ggatgagtcg gaccagacga ccgtgatcaa gcgctgtctg
     6361 aaacagctga atgtgtcagg cgacatgttc cggccgcagt cggtgttggc cgcgataggc
     6421 gcggccaaga atgagctcat ggatgtggaa gtctttcggg agagcgcgcg cgatgtgagg
     6481 caacggacca tcgtccagct atacgaggct tatcagggtg tattggcgcg cgacaatgcc
     6541 ctggatttcg acgacttaat catgattgca gtgcgtctgt tcagggagcg gcctgatgtg
     6601 ctggagaagt accgtaaccg gttcacgtac atcatggtcg tcgaatacca agacaccaac
     6661 catgcccagt acatgttggt caagctgctt gcggccagaa ctcgaaactt gtgtgtggtg
     6721 ggcgatgatg atcagtccat ttatgagtgg cggggagcgg atatccgcaa catcctggag
     6781 tttgagcggg actacccgga ggcgcgcgtg gtgaagttgg agcagaacta ccgctcaacc
     6841 cagacgattc tggatgcggc ctgtcatgtc atcgccaaca accgcgacag gaagagtaag
     6901 tcgctctgga ctgctcgcga gccgggcgcg aagatcgccc tgtacagtgc ggacacggag
     6961 aaagacgaag ctcgtttcat tgccgacgaa ctcgaccgcc tgattgtagc cgagggtcga
     7021 tgctatggcg acttcgccgt gctatacagg atgaacgcgc agtcgcgcgc tatagaggaa
     7081 gtgctggtaa acaggggaat accatacagg attgtgggag gcctcaagtt ctacgaacgg
     7141 cgcgagatca aggatgtttt ggcgtacctg cgagtgattg agaacccgga ggactcggtg
     7201 agcctggagc gaataatcaa cgtgcccagg cgtggaatag gcgattccac tttgtctgcg
     7261 gcccgggcgt tggcgcagca gaagggcctg ccgctcctcg aaggcgtgaa gatggcgacc
     7321 gagtcgacgg agctcacgac gcgggcgcga gccgcgctgg ccgatttcct tgcgacgatg
     7381 tcgcgtcttt ggccgcttcg gggcgagaaa acggtgtcgc agatcacaag agcagtgctt
     7441 gatgaaacgg gttacttggc agaactggag cgggagcaga ccattgaagc cgagggcagg
     7501 atcgagaacc tgaaggaact gatcactgtc acgcaggagt tcgagtcgag gcatgctgcg
     7561 cagggcacag gggccgaggc gggggtcggg gcgggcccgg aaggcgcgca acttgaggta
     7621 catgcgtccg gccccaacga tctcgcagcc ttcttggagg aggtttcgct cctcactgac
     7681 ctagataggg cggacctttc gactgaccag gtgacgctaa tgactttgca ttcggccaag
     7741 gggctggaat tcccggtggt cttcatggcg ggcatggaag agaacgtgtt cccgttggcg
     7801 cgttcggcct atgacccggc ggccctggaa gaagagcgca ggctatgcta tgtggggatc
     7861 acgcgcgcca aagacaggct ctatctggtt catgcgttca cccgcgctat attcggatcc
     7921 tccacagcca atccagtgtc gcggtttgtg gaggagatcc cggggcatct tctgtcggat
     7981 gcgtttgcgg agaggtatag agtctaccat ggcgggcgtg gcggggttcc gggtcgcgct
     8041 tggctcgggg atggagctgg cacgaggcct gaggcagtca gggccgagaa ggtcgcatct
     8101 ggagttgaaa gggcatgggc gacgcgccag gtgccaggca ggcccgagtt tgaggccggc
     8161 gacaggatta agcatgccaa gttcggagag ggcactgttg tgtcggtcca gcagagtgca
     8221 ggtgagtggc tcgctactat cgcgtttccc ggagcggggg tcaagcagtt tgtgctgagc
     8281 ttagcgccga ttgaaaaggt tcgctcagtc tgaacggggg tgatggagtt ggccgatgcc
     8341 ctagggcgag tgcgggaact cagagaactc attcgctatc ataaccaccg ttactacgtg
     8401 cttgacgatc ctgagataac cgacgccgaa tacgatgcgc tcatgcgcga gcttgccgcg
     8461 ctggaagagg cgcatccgga gctggccgac cccgactctc cgtcgcggag ggtaggcggc
     8521 gaggttgccg cctcatttga gccggtgatg cacgagattc ccatgctatc gctggacaat
     8581 gtgttcagcg aggaactgct tcgcgcgttc gatgcgcgtg tgcggcgcgc tactggatta
     8641 ccgagtgtgg agtatgtggc tgaaccgaaa atagacgggc tggccatttc cctgacgtat
     8701 cgagacgggc gcctttctgt gggggctact cgcggcgacg gcgaacgcgg cgagaacgtg
     8761 acggcgaacc tcaggaccat tcggtccata cctttgattc ttcctgacgg agcgccggaa
     8821 tccctcaccg ttcggggcga ggtgtacatg cgcaagcctg acttcgtcgg tctcaatgaa
     8881 gagagggaag agcgcggcga gcccgtattc gcaaatccca ggaatgctgc ggcgggatcc
     8941 cttcggcagc aggatccgca tgtgacggca tcgcgcaagc tggacgcctt cttctactac
     9001 ctgcccataa gcgagcagct cggcgccaac acgcattggg aggcgctcga acgcatgcgg
     9061 aacttgggct ttcgggtgaa tgacaagata accttgtgcc gcgacattga gcaggtcatc
     9121 gcatttattc acgacatcgc cggtatgcga gagtccttgc cgtatgagat cgacggggcg
     9181 gtggtcaagg tcaaccggtt cgatctccag agggatctcg ggttcacgtc ccgttccccc
     9241 aggtgggcta cggcgtacaa gtatccggcg cagcaggcgg agacgaccgt gcgcgacatc
     9301 gtggtgcaag tcgggcgcac aggagtgttg acgcccacgg cggagtttga gcccgtggat
     9361 gtaggcgggg ttaccgtgag ccgtgcatca ctgcacaacc aggactatgt gcgagccaag
     9421 gatattcgag tcggagatag ggtgatcata cagcgcgcgg gcgatgtcat cccggaggta
     9481 gtgagggtgc tcacggagcg ccgaaacggc ctggagcggg agttcgctat gccggccatc
     9541 tgccccgaat gcggatctgg cgtcgtgcgg gaggcgggcg aggcggctac caggtgcgtg
     9601 aacctgtcgt gtcctgcgcg catacgcgaa ggcctgatac acttcgcctc gcgcgatgcc
     9661 atggacattg atgggatggg gccgtccact gttgaaaggt tgctcgctgc aggactggtt
     9721 cgcaacgtgg cggacatcta caccctcact cgggagcagc tcaaggggat cgaacgcatg
     9781 ggggagaagt cggcggctaa tttggccgct gccattgaca atagtaaggc taacccgctc
     9841 taccgggtga tcttcgcgct gggaatccgg cacgtgggcg agaatgtggc aaagttgctg
     9901 gccggccatt tccgctccat ggatgctctg gcttgcgcga cccacgtcga tctgctgtcg
     9961 gtgcccacgg tgggcgaggc gatagccgat agcgtggtca cgttctttga gtctgaggag
    10021 aatcgggcgg ctatcgggcg gcttgcggag gcgggcgtga ggatggcgga tgaggcgccc
    10081 actggatcgg agacgccggg tgtcaacagg tcggagtttc agggaaagtc attcgtgttt
    10141 accggagcgc tcaagcggtt tacaaggcag cgggcagagg agattgcgga gatgctgggt
    10201 ggacgcgccg cgtcgagcgt gagccggaag actgactatg ttgtggcagg agaagacgca
    10261 ggatctaagt tggctcgggc gagagaactt ggagtccccg tcctttccga acaggaattc
    10321 gtggacatgc ttgatcgggc aggaatcggt gcagcgatgc agggtggtga gtaaattgtc
    10381 gttatctaga gaagagactg ctcgtctcgc ggaccttgcg aggctgagcc tcagtgatga
    10441 tgagcttgcg cggatcgctc atgacctcac ggagataatc gactacatgg agaagctggc
    10501 ggggatcgat agggcccacg ttcgacctat gtcccacgtg ctgggcacgg tgaatgtgtg
    10561 tcgcgacgat cgcgctgagc cttcgctatc ccaggaggag gcgctcgcga atgctcctga
    10621 taggcaaggc gagttcttcc gggtgccgag gatcatcgat gcaggggagg cgagcgaggc
    10681 atgagcggca gcgcagatac gaggctatgc catatgacat tgcatgaact ccgcgacatg
    10741 ctggcgcagg gccaggtgtc ggcgagagag atcacgagtg acgtcctcgc ccagatagac
    10801 gaggtggagg catcggtcgg agcatacgtg acagtggacc gcgaaggggc gctgagcagc
    10861 gctgaagcgg ccgatgcgac gatcgcccag gccaggcgtg acggcgattt cggcgcggtc
    10921 ggacctctgg ccggaatacc agtgtcgcta aaggataatg tgtgcacccg cgggatgcgc
    10981 actacgtgct gctcgaagat gctggagggc ttcgtccctg cgtatgatgc cacagtaacg
    11041 gcgcgtttgc gtgaggcagg ggcagtcatc gtgggcaagg ccaacatgga cgagttcgct
    11101 atggggtcca ctactcagac ctcagtcttt catatgacgc ggaacccctg ggacttagag
    11161 cgggttccgg gcggttctag cggcgggtgc gcggcggctg tggcggcggg cgaagccgtg
    11221 gcggccatag ggacagacac cgggggatcc atcaggcaac cggcatcttt ttgcggtgta
    11281 gtcgggctga agcctacgta cgggcgggtg agcaggtacg gtgtagtggc tttcgcatca
    11341 tcactggaca caatcggacc gcttacgagg gatgtgcggg attgtgccgt catgcttaac
    11401 gccatcgccg ggcgcgatga gcgggatgct acgtccgcgc ctgatgcagt gccggactac
    11461 accgagtgcg tggaacgagg agtacagggg ctcagaatcg ggatcccgaa ggagttcttc
    11521 gggcctggaa tgagcgaggg cgtgcgggac gcggtggagc gtgcactgcg ggctctagag
    11581 ggtgccgggg ccgatattgt cgaagtgtcg ctcccgctag tagagcatgc gctggcggta
    11641 tactacctgg tggctacggc ggaagctagt tcgaatatgg cgcggctgga cggcatccgt
    11701 tatggataca gacctgaggg gcgcggcgga atggatgtgg ccgacctcat gagcctaagc
    11761 cgcgggcagg gtttcggcat ggaagtgaag cgccgcataa tgctggggac attcgtcttg
    11821 agctccggca acttcgacgc gttttacggg cgggccctta ggtcgcggcg gcttctagcg
    11881 gaggatgtgc agcgggcgct ggcggaatgc gactgcctgg tgtcgcccac tgctccgacg
    11941 gtcgcgttca ggttcgatga ggaacccgac gatccgctgg ccatgtacct gcaagatatc
    12001 tacaccactc tggcaaacct ggcaggcgtg ccggcgatgt cggttccgtg tggactcgcg
    12061 gacggcatgc cagtggggtt gcagatcatg ggcaggatgt tcgatgaggc cacgttgatc
    12121 cgggttgggc gtgcggttga gctggtgtcg ggaatcggcg gggctaggcc cgcggttggg
    12181 gggtgcgcga agtgacggag cgagacgact acgaggttgt tatcggtatc gaagtccacg
    12241 tggagctggc gactgagtcc aaggtgttct gcggatgcaa aaccgcgttc ggcgccgcgc
    12301 caaacacgca ggtctgccct gtgtgcctag gcatgcccgg cgccctgccg atgctcaata
    12361 aacgcgcgct cgagtatacg atccgggcgg ggctggcgct gggctgcacc atcgcgggct
    12421 ggaccaagtt cgacaggaag aactacttct accccgacct tgccaaggga taccaggtgt
    12481 cgcagtacga tatccccatt gcgcgcgacg gttacatcga tatagaggtg gacgggcagc
    12541 caaagcggat acgggtcagg cgtgtgcacc ttgaggagga gaccgccaag ctagtgcacg
    12601 ccggcgacga cataatctcg gccaaccagt cgcttctaga cttcaaccgc tccgggatcg
    12661 gtctgataga gatcgtgtcg gacgccgaca tgcgatcggc cgacgaggct cgggcgtacc
    12721 tgaccaagct ccgatccatc ttggtggcta tcggcgtgtc cgactgcaag atggaggaag
    12781 gctcgatgag gtgcgaggcc aacgtgtccg tgaggcctcg gggttcgaag gaatacggca
    12841 cgttggtgga gctcaagaac atagcgtcgt tcagggcagt gcacagggcc atccagtatg
    12901 agtcggcgcg tcagatcgac ttggtacgct ccggtggacg ggtcactcgc gagacgcgac
    12961 actgggatga tgtccgcggt gaaacggcct tcatgcgcag caaggagcag gcggccgact
    13021 accgctactt ccccgagccg gacctcgtgc cgctcgaaat cgccccggag tgggtggaag
    13081 agatcaaggg cgctctgccg gaactgcccg atgcgagggc cgcaaggtac gtggcagagt
    13141 tcgggcttcc gggaagcgac gcagcgttga tagctgagtc ccgggagttg gcatccttct
    13201 tcgatggcgt atgtgatgtt cttgcgaagg cgggcgctgg ggccgtcgac cg
//
LOCUS       DAOTAC010000027        13167 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_3967_length_13167_cov_4.300641, whole genome shotgun sequence.
ACCESSION   DAOTAC010000027 DAOTAC010000000
VERSION     DAOTAC010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 13167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13167
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3967_length_13167_cov_4.300641"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /gene="hslV"
                     /locus_tag="PLM74_02145"
     CDS             complement(<1..368)
                     /gene="hslV"
                     /locus_tag="PLM74_02145"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020374732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HON41917.1"
                     /translation="MMVIEAEAKFRCTTVVGVLRDGKCALGSDGQVTLGNTVMKHGAR
                     KIRRLSDWNVLVGFAGSASDSLALLERFEGKLSEHKGNLVRAATALGREWRTDRVLQK
                     LEAMLIVTDGQRMLVLSGSG"
     gene            complement(349..1299)
                     /gene="xerC"
                     /locus_tag="PLM74_02150"
     CDS             complement(349..1299)
                     /gene="xerC"
                     /locus_tag="PLM74_02150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HON41918.1"
                     /translation="MSWADSFARHLESERNLSPRTVDAYTRDVAQFIAFCERLGIDED
                     DPRRIGVGAIRKFLSMLQTKGLSPSSVARKLSAIRSYLRYLSREGAIESNPAVVMGRA
                     RRPRRLPRALTPDEAARLISVAGGHSLQAIRDRAILELLYSSGIRLSELVGLDVQDYS
                     PGSRSVRVFGKGAKERIAPVGSQAVAAIDRYLVSARSGLLGESDCQALFMSKLGRRLS
                     GRAVERIMKKYLMKAGLPGKATPHSMRHSFATHLTDSGADLRAVQELLGHADISTTQI
                     YTSVSRERLYRVYQEAHPRAERRQAPTAGNGGGIDDGHRS"
     gene            complement(1286..2683)
                     /gene="trmFO"
                     /locus_tag="PLM74_02155"
     CDS             complement(1286..2683)
                     /gene="trmFO"
                     /locus_tag="PLM74_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009348773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HON41919.1"
                     /translation="MVTSGLKNRRDVARAGRRRARLPNEITVIGAGLAGSEAAWQAAS
                     LGARVRLVEMRPEVNTPAHSTSRFAELVCSNSLGSDREGTASGLLKEELRALGSVIMR
                     CADATRVPAGQALAVDRALFAAGVTEEIASVPQIEIVREEARHIPDGGIVVIATGPLT
                     SPALAADIARFLGDEHLHFFDAAAPIVDGASIDFEVAFWASRYDKGDADYVNCPMNQA
                     EYDSFWQALVSAEVTEMHEFESMRLFEACMPVEELARRGRDTLRFGPAKPVGLIDPRT
                     GKRPYAAVQLRRENAPGSLFNIVGFQTRLTRPEQRRVFRMIPGLAQAEFARYGMMHRN
                     TYINGPAHLAPTFSLKSVPRVFFAGQITGVEGYLESTASGLVAGINAARMAQGAEQIV
                     FPSQTAIGALCSHVSTPAAGTYQPMNISFGLLPAPGNHVRRAERRARQISDARTALAE
                     FIDEHLGGRSEHELG"
     gene            complement(2610..4745)
                     /gene="topA"
                     /locus_tag="PLM74_02160"
     CDS             complement(2610..4745)
                     /gene="topA"
                     /locus_tag="PLM74_02160"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HON41920.1"
                     /translation="MGKTLVIVESPAKVRTLSKFLGRQYTVKASIGHVRDLPKSKLGV
                     DVENGYTPKYINIRGKGDVIRELREAAKKADKVLLATDPDREGEAISWHLAHILKIDP
                     ASDCRIEFNEITESAVRNALKKPRPVDMARVDAQQARRVLDRLVGYNLSPFLWRKVRR
                     GLSAGRVQSSALKLICDREREIQKFVVEEYWTLAATLSPGARGRRFLARLMPPVEGRM
                     SSQDVNRIVGELEGAEYVVKSVTVKEQARKPSPPFTTSTMQQEAFRKLGFSARRTMSI
                     AQQLYEGVEVAGEGHVALITYMRTDSTRISHEAQAAARDFVKARYGQSYLPERPRVYK
                     SKKGAQDAHEAIRPTRIDRNPESLSGSLGRDQLRLYKLIWSRFMASQMSNAVYDATTI
                     DIDAGAGGTRHVFRATGQVMKFDGFSVLYTEGRDDDKNERDQALPALEAGQKLTLIEL
                     APEEHHTQPPPRFTEATLVRELEKHGIGRPSTYAPIIDTIRKREYATLEQKRFKPTDV
                     GLAVCDLLSEHFPTVVNLEFTASMEGELDEIEEKRSDWVQITDKFYKPFSERLREADA
                     VAERVVIADEPTDEDCPECGLAKLVIKAGRFGKFIACPRYPECTYRRSITRKVDAKCP
                     ECGGDMVQRRSKKGRVFYGCARYPECEFVSWNPPSGVVCPKCASFTTSRRVRAGVKYK
                     CANPKCGHEWAQEQEGRGAGGPEASTVAE"
     gene            complement(4802..5953)
                     /gene="dprA"
                     /locus_tag="PLM74_02165"
     CDS             complement(4802..5953)
                     /gene="dprA"
                     /locus_tag="PLM74_02165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HON41921.1"
                     /translation="MDDAKSDITWDDVPEPRDAELAAWVFWHMCPGIGPRTLWRLLME
                     FETAEAAARAATRGAKRPAFVSSEAWRFASSALDIVGAGQAEIELASRHGARVVALPS
                     QEYPPLLRRIYDPPAVLYVMGRTSLVEAPWIAVVGSRSATSYGTGAARYLSRQLAAAG
                     AGVVSGMARGVDSAAHRGAIEAGGVTAGVIGAGLAYQMTSRLECLAREVTQSGCIVSP
                     FHMTYPASRGTFPARNRVISGMAHACLVVEAGARSGALVTSTFATEHNRDVFAVPGDI
                     NRPTSAGTNALIYDGARPVIGARRFLEELAAAGVPVTLPDPGNAGRGQNLGPVDAAVL
                     EAVRSDTSFEDIHLALPMPARDVLTSLSRLEVAGLIVRGAGLRYSRRHC"
     gene            complement(5946..7457)
                     /locus_tag="PLM74_02170"
     CDS             complement(5946..7457)
                     /locus_tag="PLM74_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005581806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HON41922.1"
                     /translation="MGITGVSVAVEADLARGLPAFDIVGLPDSTVRESRERVRAAIRN
                     SGYDFPLAKITVNLAPAHIRKVGVGLDLPVAVSILAASGQVPARNLNCTTFMGELSLD
                     GALRPVRGVLPGCAAALGAGNSCVVVPFENAREAECVSGLDVKALGTLHEVVEYLRSG
                     AIPSFVRSHPACPQPPESIGSCDFDQVVGQDAGIRAIQVAVAGGHNLIMVGPPGGGKT
                     LLASCVPSILPAMDEHEALEVTCIQSITGTVSPDGLASKRPFRRPHHTVSPAGMLGGG
                     NPPMPGEVTLAHRGVLYLDEFCEFRPQVIESLRGPMEDGFIVVSRSHGRVSFPSRFML
                     IASANPCPCGYLGDPERACVCQGASLMRYRRRLAGPVIDRIDIQIEVPRVRRIDLERP
                     VAVLTSDAVREEIERARARQGGRFGRKGFTNSAIGPRQIESVARLAPAARRLLLDAVD
                     KMALSARAYHKLIRVSRTIADLDGADSVEDGHVLEALQYRALDLQPQAMLSYG"
     gene            complement(7542..7907)
                     /locus_tag="PLM74_02175"
     CDS             complement(7542..7907)
                     /locus_tag="PLM74_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011692316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HON41923.1"
                     /translation="MRAQDVGRAGEVLAREYLVSLGYEILGCNLRLGRNEIDILARDG
                     DVLVVVEVKTRRSTAFGRPEEQITLGKLRTLRLVATRVSAACRGAPVRVDVVAVMADA
                     ATGESLAGAAISHLRNVLT"
     gene            complement(8019..8633)
                     /locus_tag="PLM74_02180"
     CDS             complement(8019..8633)
                     /locus_tag="PLM74_02180"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HON41924.1"
                     /translation="MINRAALGEFDDCLRRSGFAVLAGIDEAGRGPLAGPVVAAAVVM
                     RPGFHPQDVIDSKMLSAARREAALQRILEACVEVSLGAAWPDEIDEINIRAATLSAMS
                     RAMSQLTVSPDLALVDGRDLPAMDCPGVALVRGDARSESVAAASIVAKVGRDAIMCQL
                     HEVYPDYGFDRHKGYPTKEHLQRLAVFGPCPLHRRSYRGVERTG"
     gene            complement(8630..9469)
                     /gene="ylqF"
                     /locus_tag="PLM74_02185"
     CDS             complement(8630..9469)
                     /gene="ylqF"
                     /locus_tag="PLM74_02185"
                     /inference="COORDINATES: protein motif:HMM:TIGR03596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HON41925.1"
                     /translation="MGLSWFPGHMAKAVGEIRQSMKLIDVVIEILDARAPRATANPYL
                     DEALGLKPRVVVLNKADLADARATQAWLDALREASSRAVQADCQKGAGVNEAISLAEQ
                     AARSTGLDSGGQRRRRRFRTAVVGIPNVGKSSFINRAARRSGARTGDRPGVTRAKQWI
                     VVSPSLEMLDTPGIMPPRVDDPAIWFALAAVGCIDDNLLDIESLSYNLISRLAELGAV
                     EFRARYGVPDDMVDPHIILEHVSLKRGCLKSGGEADTERGAGLIVRDFRSGKLGRVTL
                     EEP"
     gene            complement(9552..10166)
                     /gene="lepB"
                     /locus_tag="PLM74_02190"
     CDS             complement(9552..10166)
                     /gene="lepB"
                     /locus_tag="PLM74_02190"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HON41926.1"
                     /translation="MRRAAATFLQFAGLMLIIAVLTAFAVTFVAMAVVVQGSSMEPAL
                     HDGERVLVNRLVYKLRHPRRGEIVVFRHGHMPGSMLIKRVIGMPQEELSIVRGLVHVN
                     GAPITEDYTLERSAQDYGPVYVPDGRLFVLGDNRNHSEDSAHASIGTVPLRSIEGKAF
                     AVYWPLASVRMCRDPGYPESAEAAITATTAQISVSGAARVSSAP"
     gene            complement(10169..10522)
                     /gene="rplS"
                     /locus_tag="PLM74_02195"
     CDS             complement(10169..10522)
                     /gene="rplS"
                     /locus_tag="PLM74_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HON41927.1"
                     /translation="MNIIDTIEREQMRKDLPDFAPGDTVRVHVRVVEGGRERIQVFEG
                     VVIGRNGSGASETFNVRKLSAGIGVERLFPLHSPKIDKIEVVRFGDIRRAKLYYLRGR
                     VGKRARIRERKRERV"
     gene            complement(10586..11323)
                     /gene="trmD"
                     /locus_tag="PLM74_02200"
     CDS             complement(10586..11323)
                     /gene="trmD"
                     /locus_tag="PLM74_02200"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HON41928.1"
                     /translation="MEVDILTIFPGMFSGVMSESILGRAQSKGILTVRVHDIRDFALG
                     KHRIVDDYPFGGGPGMVMKPEPIAAAIEHALLCRESSGGGESRVVLTTPAGRPFTQSL
                     AREYSACEHLVILCGHYEGIDERVSTLVSDEVSVGDYVLTGGEIPAMVIVDATARLLP
                     GALGDASSAAEDSFWDGLLDYPHYTRPRLWRGLEVPDVLISGDHERIRRWRREQSLER
                     TRCRRPDLLEQAELTREDLKMLQNLAP"
     gene            complement(11333..11917)
                     /gene="rimM"
                     /locus_tag="PLM74_02205"
     CDS             complement(11333..11917)
                     /gene="rimM"
                     /locus_tag="PLM74_02205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HON41929.1"
                     /translation="MNPERGRLVLVGWIVGTHGVRGEVRVKPCAGSPDRFRGVARAFL
                     GPADAEADVDADADVGVGAGVCAGRNEYRPVDLESARPHGSLALLRIAGIADIDSALA
                     MRGMGLYLPASELPRLPEGEFYSFELVGLTCESTTGERLGVVVEVLEMPANDVLLVRP
                     EEGPDFLVPAANDIVHQVDLAGGRLVIDDRPGLR"
     gene            complement(11914..12174)
                     /locus_tag="PLM74_02210"
     CDS             complement(11914..12174)
                     /locus_tag="PLM74_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014042683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HON41930.1"
                     /translation="MAIGKKNRVELKDLVSYLASNLVDEPDTVRVREIQNGDSVILEV
                     SVAPDDMGKVIGRHGRVARAIRTVTKAAAARQGRRAMVEIVE"
     gene            complement(12187..12492)
                     /gene="rpsP"
                     /locus_tag="PLM74_02215"
     CDS             complement(12187..12492)
                     /gene="rpsP"
                     /locus_tag="PLM74_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HON41931.1"
                     /translation="MAVRIRLTRTGAAKQPHYRLVVADSRSPRDGRFIEIIGHYNPRM
                     DPPELVVDVEKAAEWIKRGARPSDTARALLAKAGLRGTTRGSQSDKASEPEAEPVRE"
     gene            complement(12552..>13167)
                     /locus_tag="PLM74_02220"
     CDS             complement(12552..>13167)
                     /locus_tag="PLM74_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HON41932.1"
                     /translation="QGVILTKLDGDARGGAALTVREVTGKPIKFVTTGEKLDALEQFH
                     PDRMSSRILGMGDVLTIIEKAQETFDLEQAKKLEEKLRKQEFTLDDFMRQLQDVRKMG
                     PLDQLLGMIPGVARSKALKDVRVDERQFTKVEAIIRSMTPAERKNPASIDGSRKRRIA
                     AGSGTRVQEVNALLKQYDQARKMLRQLGDASKRGGPMGKVPFMP"
BASE COUNT         2509 a         4371 c         3608 g         2679 t
ORIGIN      
        1 tcgccgctgc ccgacaggac cagcatccgc tgaccgtccg tcactatcag catggcctcg
       61 agtttctgaa gcactcggtc ggtgcgccat tcgcgcccca gagcagtagc tgcacgtacc
      121 agattgccct tgtgctccga aagctttccc tcgaaacgct cgagaagagc caaagaatcg
      181 gaagccgaac ctgcaaagcc tactagtacg ttccagtcgg acaacctccg tatcttgcgc
      241 gcgccatgtt tcatcaccgt gttgcccaaa gtgacctgac catctgagcc aagggcacac
      301 ttgccgtcgc gcagcactcc cacgacagtg gtgcacctga acttggcttc agcttcgatg
      361 accatcatct atcccccccc cattccccgc cgtcggcgcc tgccggcgct cagctcttgg
      421 atgggcttcc tggtagacgc ggtaaagtct ttcccgcgaa acgctggtgt aaatctgagt
      481 agtggagata tctgcatgcc ccagaagctc ctggactgca cgcaagtcgg caccggaatc
      541 agtaaggtga gtggcgaatg aatgcctcat cgaatgagga gtagccttcc cgggaagacc
      601 cgctttcatc aggtatttct tcatgatgcg ttccacggcc cgccccgaca ggcgccggcc
      661 aagcttgctc atgaagagcg cctggcagtc gctttcaccg agcagcccgg aacgagcgct
      721 cacgaggtac cgatcgatgg cggccactgc ctgcgatcca accggcgcta tcctctcctt
      781 agcgcctttg ccgaacaccc tcacgctgcg acttcccggc gagtagtcct gcacatccag
      841 gcccaccaac tcagacagac ggatgcccga cgaatacagg agttccaata tggcacgatc
      901 ccgaatggcc tggagcgaat ggccaccggc gaccgaaatc aggcgcgccg cttcatctgg
      961 ggtcagtgcg cggggaagtc gccggggtcg cctggcgcgg cccatcacaa cggctggatt
     1021 actctcgatc gcgccctcac gtgaaaggta tcgcaagtaa gaccgtattg cgctgagttt
     1081 cctcgcgacg ctgcttgggc tcaggccctt ggtctgaagc atggacagga acttccgtat
     1141 ggcgccgact ccgatccggc gcgggtcatc ctcgtcgatg cccagccgtt cgcagaatgc
     1201 tatgaattgc gcgacatctc tagtgtaagc gtcgacagtc ctgggggaca gattacgctc
     1261 cgactcaagg tgccgggcga aactatcagc ccaactcatg ttcgctcctc ccacctaggt
     1321 gttcgtctat gaactcggcg agggccgtcc gcgcatcgct tatctgacgc gctctccttt
     1381 cggctcgccg aacgtggttg ccgggcgccg gcagcaagcc gaagctgatg ttcatcggct
     1441 gatatgtgcc ggccgcgggc gtagatacat gcgagcagag ggcgcctatg gccgtctgac
     1501 tcggaaatac gatctgttct gctccttgcg ccatacgggc ggcattgatc cctgcaacaa
     1561 ggcctgaagc ggtggattcg agatagccct ctacccccgt gatctgccct gcgaaaaaca
     1621 ctcttggaac gctcttcagg gaaaacgtgg gcgcgaggtg tgccgggccg ttgatgtatg
     1681 tgttcctatg catcatgccg taacgcgcga actcagcttg cgcaagcccc ggtatcatac
     1741 ggaatactcg tctctgctca ggcctggtaa gcctggtctg gaaacccaca atgttgaaca
     1801 gcgagcccgg ggcattctcg cgcctgagct gcactgcagc gtaaggacgt tttccagttc
     1861 gcgggtcgat caaccccacg ggttttgcgg ggccgaagcg aagagtgtcg cgcccgcggc
     1921 gagccaactc ctctacgggc atgcacgctt cgaataggcg catggattcg aactcgtgca
     1981 tctcggttac ttctgccgaa accagggcct gccagaacga atcgtattcg gcctgattca
     2041 tggggcagtt aacatagtcg gcgtcgcctt tatcatacct agaagcccaa aaagccacct
     2101 cgaagtcaat ggaagccccg tcgactatgg gtgcggctgc gtcgaagaag tgcaggtgtt
     2161 cgtcgccgag gaagcgcgct atgtcggctg cgagcgcagg cgaagtgagc ggacctgtgg
     2221 ctatgacgac gattccgccg tcaggtatgt gccgcgcttc ctcgcggact atctcgatct
     2281 gaggtacgga tgctatttcc tccgtcactc ccgctgcaaa cagcgctcgg tcgactgcaa
     2341 gggcctgccc tgccggcacg cgcgtcgcgt cggcgcatcg catgatcaca gatcccaatg
     2401 cgcgtagctc ctccttgaga aggcccgacg cagtgccttc tctgtcggaa ccgagggagt
     2461 tgctgcacac aagctcagcg aaacgggagg ttgagtgagc cggggtgttg acctccgggc
     2521 gcatctcaac cagcctcact cgagccccga ggcttgcggc ctgccacgcc gcctcgctgc
     2581 cggccaaacc cgcccctatt acagtgatct cattcggcaa ccgtgctcgc ctccggcccg
     2641 cccgcgccac gtccctcctg ttcttgagcc cactcgtgac cacacttggg gttggcgcac
     2701 ttgtacttga ccccagcgcg cacccgcctg gaagtagtga aagatgcaca cttggggcag
     2761 acaacaccag atggggggtt ccatgacacg aactcgcact ccgggtatcg ggcgcacccg
     2821 tagaacaccc ggcctttctt gctgcgcctc tgcaccatgt cgccgccgca ctctgggcat
     2881 ttagcatcga ccttgcgggt tatgctcctg cggtaggtgc actcggggta gcgtggacaa
     2941 gcgatgaact taccgaacct acctgcctta atgaccagct tggctaggcc gcactcaggg
     3001 caatcctcgt cagtgggttc atccgcgatg accacgcgct ccgccacggc gtccgcctcc
     3061 cggagccttt cggaaaatgg tttgtagaat ttgtcggtta tctgcaccca atccgatcgc
     3121 ttctcctcga tttcatcgag ctcaccttcc attgaggccg tgaactccag attgaccacc
     3181 gtcggaaaat gctcgctgag aagatcgcac acggccaatc ctacgtcggt gggcttgaaa
     3241 cgtttctgct ccagcgtagc gtattcgcgc ttgcgaatag tatcgattat gggcgcgtac
     3301 gtactgggcc ttcctatccc gtgcttctcc agttcccgga ctaaagtagc ctcggtgaac
     3361 cttggcggtg gttgggtatg gtgctcctcg ggggcaagct caataagggt caacttctgc
     3421 cccgcctcga gcgctggcag cgcttggtca cgctcattct tatcgtcgtc tcgcccttcc
     3481 gtgtacagta cgctgaaccc gtcgaacttc atcacctgcc cggtagcgcg gaagacgtgc
     3541 ctcgtaccgc ctgcgcctgc gtcaatatcg atggttgtgg cgtcgtacac cgcattcgac
     3601 atctggctgg ccatgaagcg cgaccatatg agcttgtaaa gcctgagctg atcgcggccg
     3661 agactgcccg ataggctctc gggattccga tcgatccgtg ttggcctgat ggcctcgtga
     3721 gcatcctggg cgcccttctt ggacttatag actcggggtc gctcaggcag atagctttgg
     3781 ccgtatctgg ccttgacgaa atcacgcgcc gccgcctgcg cctcgtggga tatccttgtt
     3841 gagtcggtac gcatgtatgt aatgagcgcc acgtgccctt cgcccgctac ctcaacacct
     3901 tcgtacagct gctgtgcaat ggacatcgtt cgtctggcgg agaagcctag tttcctaaag
     3961 gcctcttgct gcatagtact ggttgtgaac ggaggcgacg gctttctggc ctgctccttg
     4021 actgtcaccg acttgacgac atattcagcg ccttccaact cgcccacgat tctgttgaca
     4081 tcctggctcg acatgcgccc ttcaactggg ggcatcagcc tggcaaggaa tctcctgcct
     4141 cgagcgcccg gcgaaagcgt ggcggcgagt gtccaatact cctccacgac aaacttctgt
     4201 atctcacgct cgcggtcaca gatcagcttg agggcggatg actgcactcg cccggccgac
     4261 agtcctcgcc gaaccttgcg ccagaggaac gggctgaggt tgtagcccac cagcctgtca
     4321 agcacccgtc tggcctgctg cgcgtctacc cttgccatgt cgacgggcct gggtttcttg
     4381 agcgcattcc gaactgcgct ctcagtgatc tcgttgaact cgatcctgca gtctgacgcc
     4441 ggatcgattt tgagtatgtg cgccaagtgc caggaaatgg cttcaccttc gcggtcgggg
     4501 tcagtggcaa gaaggacctt atcagccttt tttgcagcct ccctcagctc gcggatgaca
     4561 tcccccttgc cccggatgtt tatgtacttg ggagtgtagc cgttctccac gtccactccc
     4621 agtttgctct tgggcaggtc cctgacatga ccgatggacg ccttgactgt atactgtcgc
     4681 cccaagaact ttgacagagt cctaactttg gccggtgact ccacgataac gagagtcttc
     4741 cccatgcggc cacctccaac gttcgatgtc acgaacagtt taacccaggg cacatgcgag
     4801 gtcaacaatg cctccggctg tatcgcaggc cggcgccgcg cacgataagc cctgcaacct
     4861 ccagacgcga gagcgaggtt agcacatcgc gcgcgggcat tggcagcgcc agatggatgt
     4921 cttcaaacga cgtgtcagac cgaactgcct ctagtaccgc tgcgtctacc ggcccgagat
     4981 tctgccctcg cccggcattc ccagggtctg gcaacgtcac cggtacacct gcggccgcca
     5041 gctcctcgag gaatctccgt gcaccgatca cagggcgcgc tccatcgtat atgagggcgt
     5101 tagttcctgc cgaggtaggt cggttgatgt cgccgggtac agcgaagaca tcgcggttgt
     5161 gctccgtcgc gaaggtcgac gtgaccaagg cgccgcttct ggcccccgcc tccaccacga
     5221 ggcacgcatg agccataccg ctaatgaccc gattgcgcgc gggaaacgtg ccgcgagacg
     5281 ccggataagt catgtgaaac ggtgacacaa tgcagcccga ctgcgtcact tcccgtgcca
     5341 aacactcgag cctcgacgtc atctggtacg caagccccgc gccgatcacg cccgcggtca
     5401 cccctccggc ctcaatcgcc cctcgatgcg ctgcagaatc aacgcctctt gccatgccgc
     5461 tgaccacgcc ggcgcccgcc gccgccagct gacgcgagag gtatctcgcg gcgcctgttc
     5521 cgtaggatgt agccgaccta gacccgacca ccgctatcca cggcgcctcc actagcgacg
     5581 tcctgcccat cacgtatagc acggccggag gatcgtagat ccggcgaagc aagggcgggt
     5641 actcctggct cggcaaggcg acgaccctgg ctccatgcct cgacgccagc tcaatttcgg
     5701 cctgccctgc tcccactatg tcaagcgccg acgacgcgaa cctccaagct tcactactca
     5761 cgaaagccgg gcgtttcgcg cctcgggtcg cagcgcgcgc tgccgcctcg gccgtttcga
     5821 actccatgag caggcgccat agagtacgcg gacctatccc cgggcacatg tgccagaaga
     5881 cccacgcagc gagctcggca tcgcgaggtt cgggcacgtc atcccatgtt atatccgatt
     5941 tcgcgtcatc catatgacaa catcgcctgc ggctgcaaat ccaaagccct atactgcagg
     6001 gcctccaaca cgtgcccgtc ttccacgctg tcggcgccgt caagatcggc tatggtcctc
     6061 gacacccgga tcagcttgtg gtaagcccgg gccgacaggg ccattttgtc gactgcgtcc
     6121 agcagcagcc tgcgtgccgc cggggctaga cgggccacgc tctcgatttg cctgggccca
     6181 atagccgagt tggtgaatcc cttccgaccg aagcgccctc cctggcgcgc ccttgcgcgc
     6241 tcaatctctt cacgcacggc gtcggacgta aggactgcaa cgggcctctc aagatcgatc
     6301 cgcctaacac ggggcacttc tatctgaatg tcgatacggt cgatcaccgg accggccagc
     6361 ctgcgtcggt aacgcatcaa ggacgcgccc tgacagacgc atgcccgttc agggtctccg
     6421 aggtagccgc agggacaggg attcgccgag gcaatcagca tgaaccgaga cgggaagctg
     6481 actcggccgt gggagcggga cactacgatg aacccgtctt ccatggggcc tctgaggctc
     6541 tcgataacct gtggcctaaa ctcgcagaac tcatcaagat acagaacgcc tctgtgcgcc
     6601 aaggtcactt cgccgggcat gggagggttt ccgcctccta gcatgcccgc ggggctcacg
     6661 gtgtgatggg gacggcggaa cggacgcttg ctggcgaggc catcggggga tactgtgccg
     6721 gtgatactct gaatgcacgt gacctccaac gcctcgtgct catccattgc cggcagaata
     6781 gatggcacgc acgatgcaag caacgtcttt ccgcctcccg gagggcccac cattatcagg
     6841 ttatggccgc ctgctactgc tacctggatt gctctgatcc ctgcgtcttg ccccacgacc
     6901 tggtcgaaat cgcatgagcc gattgactcg ggcggctgag gacacgccgg atggctccgg
     6961 acgaagctgg ggatagcgcc ggagcgcaga tattccacta cttcgtgcaa cgtacccagg
     7021 gccttaacgt caagccccga tacgcactct gcttcgcgtg cgttctcaaa cggcaccaca
     7081 acacaggaat tgcctgctcc cagggcggcc gcgcagccgg gcaggacacc gcgaaccggc
     7141 ctgagggcac catccagcga cagttcgccc atgaacgtgg tgcaatttag gtttcgcgcc
     7201 ggcacctgcc ccgatgcggc aagtatcgaa acagccaccg gtagatccaa ccccaccccg
     7261 accttcctga tatgggcggg agccaggttc acggtgattt tggcgagcgg aaagtcgtat
     7321 cccgagttgc gtatggccgc ccgcactcgc tcgcgagact cgcgcactgt ggaatcaggg
     7381 aggccgacga tgtcgaatgc cggcaatcct cttgcaagat cggcctctac cgccacagaa
     7441 acgccggtga ttcccatgac tgcacatcct ccagctgtcg aaagcatcgt acgcgcggat
     7501 cgcctcctcc tgcccatgct tgcgcgcggt tggccctctt ctcatgtcaa gacgttccgc
     7561 aagtgcgaga tggcggcgcc ggccagggat tcgccggtcg cagcgtcggc catgaccgca
     7621 acaacgtcca cgcgcacggg cgcgccgcgg caagccgcag acactcgtgt agccaccagt
     7681 cgcagcgtgc ggagcttgcc cagggtaatc tgctcctcag ggcgaccgaa ggcagtcgag
     7741 cgtcgagtct tcacctctac tactaccagc acatctccgt cacgagctaa gatgtcgatt
     7801 tcgttacgac ctaggcgcaa gttgcacccg agtatctcgt agccgagaga tacaaggtat
     7861 tccctggcga ggacttcgcc cgccctaccg acatcctgag cccgcattcg cctgcctcct
     7921 cctataccgc tgattcgacg gcttcgacct atatccttcc acaaacgaca aaatcaccct
     7981 ttcgggtgat cctgcagatg tcgtgcgctg taacagaact agcctgtcct ctccacaccc
     8041 cggtaactcc ttctgtggag cggacatggt ccgaatacgg ccaggcgctg gagatgctcc
     8101 tttgtggggt atcccttatg tcggtcgaag ccgtagtcgg gatacacctc atgcagttgg
     8161 cacatgatag cgtctctgcc aaccttggcc actatggacg cggcagccac gctttcgctt
     8221 cgagcgtcgc ccctgaccag agccactccc gggcagtcca tcgccggcaa atcgcgaccg
     8281 tctacgaggg caaggtccgg cgacactgta agctgcgaca tcgctctgga catggctgaa
     8341 agcgtcgcgg ccctgatgtt gatctcgtcg atctcatcgg gccaggcggc cccgagactc
     8401 acctccacac atgcctccag tatccgctgg agggctgcct cgcgtctagc tgccgacagc
     8461 atcttcgagt cgatcacatc ctgtgggtga aaaccgggac gcatcacgac cgcagcggcc
     8521 accacagggc cggccaacgg ccccctccct gcttcatcta tgcctgctag gacagcaaat
     8581 cccgacctac gcaggcaatc gtcgaactcg cccagcgcgg cgcggttaat catggttcct
     8641 ccagcgttac ccggccgagc ttgcccgacc tgaagtctct gacgatcaga ccggcgcctc
     8701 tttcggtgtc agcctctccc cctgacttga gacacccacg tttcagtgat acgtgttcga
     8761 gaattatgtg cggatcgacc atgtcgtcgg gaactccgta tctggctcta aactcgaccg
     8821 cgcccagctc cgcgaggcgc gatatcaggt tgtatgagag gctctcaatg tcgagcagat
     8881 tgtcgtcgat acagcccaca gcggccagag cgaaccagat cgctggatcg tcaactctcg
     8941 gaggcatgat tccgggagtg tcgagcatct ccagactggg actgaccaca atccactgct
     9001 tggcgcgggt aacgcccggt cgatcgcctg tccttgcacc cgatcgacgt gccgcacggt
     9061 taatgaacga ggatttccct acattgggta tgcctactac cgccgtacgg aaacgtcgtc
     9121 gtcgccgctg gccgccactg tctagccccg tagatcgggc cgcctgctcg gccaagctta
     9181 ttgcctcatt tacccctgcg cctttctgac aatcggcctg caccgcccgt gaggacgcct
     9241 ctctcaaagc gtccagccac gcctgcgtgg cgcgtgcatc ggccaggtcg gctttgttca
     9301 gcacgaccac tctgggcttt agacccagag cttcatcaag gtacgggttg gcggtggcac
     9361 ggggggcccg ggcgtccagt atctctatca ccacgtctat tagcttcatg ctctgacgga
     9421 tctcgccaac agctttggcc atatgtcccg gaaaccagct gaggcccatc gtccactccc
     9481 ctccctactt tcgttctgta gtacacaagc gccgggccca ggtgtagccc ggcccggcct
     9541 gaagcgttcg gtcacggcgc gctcgatacg cgggccgcgc cactcaccga tatttgggct
     9601 gtcgttgcag taatcgctgc ctcggccgac tcggggtagc ctggatccct gcacatcctg
     9661 acactggcca gtggccagta cacagcgaaa gcctttccct caatgctgcg cagcggcacc
     9721 gtgcctatgg aggcgtgcgc gctgtcttca ctgtggttcc tgttatcacc caacacgaat
     9781 aggcgcccat cgggcacgta caccgggccg tagtcttggg ccgaacgctc gagggtgtag
     9841 tcttccgtaa tcggcgcgcc gttcacgtga acaagccctc taactatcga aagctcctcc
     9901 tggggcatgc cgataactcg ctttatcaac atgctccctg gcatatgccc gtgtcggaac
     9961 acaacaatct cgccgcggcg cggatgcctc agcttgtaca ccagcctgtt gaccaggacc
    10021 cgttctccgt cgtgcaaagc aggttccatt gaactgccct gcactacgac tgccatggcc
    10081 acaaaggtga cagcaaatgc tgtcaagacc gcgataatga gcatgagccc ggcgaactga
    10141 aggaaggttg ccgcggctcg cctcatgcct atacccgttc ccgcttgcgc tctcggattc
    10201 tggccctctt gccgacgcga ccgcgtaggt agtacagctt ggcccggcgg atatcgccga
    10261 atcggactac ctcgatcttg tcgatcttcg gcgaatgaag aggaaacagc ctctccacgc
    10321 ctattcccgc cgacagcttc ctaacgttga aggtctcaga agcgccgctg ccgttcctgc
    10381 cgatcacgac gccctcgaac acctgtatac gctcgcggcc gccctcgaca acccggacat
    10441 gcactctgac ggtatcgcct ggggcgaagt ccggcagatc tttgcgcatc tgctcccgct
    10501 ctattgtatc gatgatgttc acagtaatcc cactcctcac ccggtcttca acagacgtca
    10561 gtatatcaca aggaaactgg cccaatcaag gcgcgaggtt ctgcagcatt ttcaggtcct
    10621 ctcgcgtcag ctctgcctgc tccagcaggt cgggcctacg gcacctggta cgctcaaggg
    10681 attgctcacg ccgccaccgc ctgattctct catgatcgcc cgagatcaac acgtcgggca
    10741 cctcaaggcc ccgccatagc ctgggccttg tgtagtgcgg gtagtccaac aacccatccc
    10801 agaaggagtc ctcggcggcg ctggacgcgt ccccgagagc gccgggcaaa agacgcgccg
    10861 ttgcgtccac gatcaccatc gcagggatct cgccgcctgt caacacgtaa tctccaaccg
    10921 acacttcatc ggagacaagc gtactgactc gttcgtctat gccttcgtag tgcccgcaca
    10981 aaatcacgag gtgttcgcat gcggagtact cccttgccaa gctctgagtg aacggacgcc
    11041 cggcgggtgt ggtcagaacc acccgcgatt ccccgccccc gctggattct ctacacagta
    11101 gagcatgctc gatggccgcg gcgatcggtt ccggtttcat gaccatccca ggcccaccgc
    11161 cgaatgggta gtcatctact atccggtgct tgcccagcgc gaaatcgcgg atatcatgga
    11221 ccctcactgt cagaatccct ttactctgag ctcgcccgag aatgctctcg ctcatgacgc
    11281 ccgagaacat cccagggaag atcgtgagga tgtcgacttc catggtatgg cctcacctca
    11341 acccgggtct gtcgtcgatg acgagccgcc cgcccgccag atccacttga tgcacgatat
    11401 cgttcgccgc cggcaccagg aaatccgggc cctcttccgg cctgaccaac agaacatcat
    11461 tggccggcat ttcgagcact tcgacgacaa cgccaaggcg ctcgccagtg gttgattcgc
    11521 acgtcaaccc cactagctcg aagctgtaga actcgccttc tggtaaccgg ggcagctcgg
    11581 aagcaggcaa gtataagccc attcccctca tggccagcgc tgagtcgata tcggctatgc
    11641 ccgcaatcct gagtagcgcc agagacccgt gcggtcgggc cgactcgagg tcaacgggtc
    11701 tgtattcgtt cctgcccgca cacacgccgg cacccacacc cacatctgca tctgcatcga
    11761 catccgcttc cgcgtcagcg ggcccgagga acgcccgagc aactccacga aaccggtccg
    11821 gcgaaccggc acacggcttc actcgaactt cgccgcgcac cccatgcgtg cctacgatcc
    11881 aaccgaccag aaccagccgc ccccgctccg ggttcattcc actatctcga ccatggcccg
    11941 cctgccttga cgtgctgcag ccgcttttgt gactgtgcga atggcccggg ctacccgccc
    12001 atgcctacct attaccttgc ccatgtcatc aggtgcaacc gacacctcaa ggatcactga
    12061 atcgccgttc tgaatctctc tcactcgtac ggtgtccggc tcatccacga gattagaggc
    12121 caggtaagac actaaatcct taagctccac cctgttcttc ttgcctatag ccatagactc
    12181 caccagctac tcccgcaccg gctcggcctc tggttcagaa gccttgtcgg actgggatcc
    12241 tctagtcgtt ccccggagcc cggccttggc aagcagagcg cgggcagtgt cggatggacg
    12301 cgctccgcgc ttgatccact cggcagcctt ttccacgtcg acgacgagct caggcgggtc
    12361 catgcgaggg ttatagtgcc cgatgatctc gatgaagcgt ccatccctgg gcgatcggga
    12421 atctgcaacc accagcctgt aatggggctg tttggcagcg cctgttctgg ttagcctgat
    12481 acgaacggcc attttggttc ctacacctcc gaacaactga ttcacattac ttggtatcct
    12541 gcgtcattca tctatggcat aaatgggacc ttacccattg ggccaccgcg ttttgaggca
    12601 tcgcccagct gcctgagcat cttgcgggcc tgatcatact gcttaaggag tgcgttgacc
    12661 tcctgaaccc tcgtgccact gccggcggct atccggcgct tcctcgagcc atcgatactg
    12721 gcggggttct tcctttcggc cggcgtcatc gatctaatga tcgcctctac cttcgtaaat
    12781 tgcctctcgt ccacccggac atccttgaga gccttagacc tggcaactcc gggaatcatg
    12841 ccaagcagct gatcgagagg ccccatcttc cgaacatcct gaagttgccg catgaagtca
    12901 tccaacgtga actcctgttt ccgcagcttc tcctcgagct ttttggcctg ctccaggtcg
    12961 aacgtctcct gcgccttctc gattatggtc agcacatcgc ccattccaag tatcctggag
    13021 gacatgcgat caggatggaa ctgctcgagg gcgtcgagct tctcgcctgt agtaacaaac
    13081 ttgatcggct tgccggtgac ctccctcacc gtgagcgcag ccccgccgcg ggcatctccg
    13141 tcgagcttgg tgaggatcac tccctgg
//
LOCUS       DAOTAC010000028        13103 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_3990_length_13103_cov_4.130595, whole genome shotgun sequence.
ACCESSION   DAOTAC010000028 DAOTAC010000000
VERSION     DAOTAC010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 13103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13103
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3990_length_13103_cov_4.130595"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..849)
                     /locus_tag="PLM74_02225"
     CDS             complement(118..849)
                     /locus_tag="PLM74_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HON41933.1"
                     /translation="MMKRYIVNSVSSLVSIYMIFILIFAGLQSVSGYVKTGALDGSFE
                     GTIVGFFVWTFTIYAFSNLSWNLINEAQTGTLEQLYLAPCGFKWVGAATLMSDFVFSF
                     IPIMLLLMAMMATTGRWLKVDLVTLIPLLFITLLGAYGVGFALGGLALVFKRIQALFQ
                     IIQFVFIGLLVIPIRVPGVKYLPLALGNDLIYRSMVDGVRLWRLPLGDILTAVAVGAA
                     YLAAGIAVFSWCERIAKNRGLLGHY"
     gene            complement(894..1868)
                     /locus_tag="PLM74_02230"
     CDS             complement(894..1868)
                     /locus_tag="PLM74_02230"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON41934.1"
                     /translation="MSTLDTALQVQSLTKVFRDRGGVTVRAVDGVCFDVKRGEVLGLL
                     GPNGAGKTTSIKCILGLMRPDSGRIALLGQDIQAAYPRILGKMSAVLEGSRNLYWRMS
                     VWENVVFFAALHGVRSREDREYFGHLLKLFALEDKRHTEVRKLSQGMKQKASVVCALA
                     RRTPLVFLDEPTLGLDVETSLELRSVLKQLARDEERTFVVSSHDMDVIQDVCGRVVIM
                     SSGRVVVDDKVSHLMSLFRTKAYRIGLRGPAPQGLKERLAQEFASVQVTDGDADSRVK
                     VSLSEDGDIYRLMDILRGADAIIDEITHEEVDLEKVYLEIVREERARS"
     gene            2220..3263
                     /locus_tag="PLM74_02235"
     CDS             2220..3263
                     /locus_tag="PLM74_02235"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HON41935.1"
                     /translation="MHSTIYDVAERAGVSAKTVSRVINNSPLVAPDTRRRVLAAIAEL
                     DFHPDSAAKGLRQRSRKSVGFVIPYGSDFVFQDQGMWLQTNGAHKALAERGYDLVLAV
                     PQTPELVLAELNRLTRNRTVDGVILYAMEGADALLREFDRLNIRYMSLSMCYPGQKNN
                     FVDMDSPYTAHLATKYMIDQGARRIGLISEPERFFYSAKASSEPGYRLAHEQAGLPVD
                     ESLILAGDYSIESGYEMAKRLLTSRPAVDGVICMSDPMALGALRAVREQGLVPGRDID
                     VIAGDDFPTTRAMWPGLTSIRTPLYEQGYQAGVMIVDYIQSGKEIPGKLLRGELIVRE
                     SVRSRNGIREGLG"
     gene            3263..4249
                     /locus_tag="PLM74_02240"
     CDS             3263..4249
                     /locus_tag="PLM74_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HON41936.1"
                     /translation="MLWQSGSTLLTQSDRRSAGKAGALSGWSRRQHRTAYLMILPAVV
                     AMLLVHYMPMLWGFYISLHDVNLFTMADWMNAPFIGLRNFVDAFDPSTTTGARALRAL
                     LNITVYSAVTVSVGYLIGLAVALLLNQQFWGRTFVRGLILLPYITPDSVAYKVWTFIF
                     QSRIGILNRYLIQWGLVSEPPIWLIGPPALWAVVAASVWKGWPFSALILLAALQKIPQ
                     DLYEAARVDGASIWQQFAWITMPHLQSVTVTLLLMSILWNFNAFNQFYVMLGRDPGVA
                     AEVPSTLILREAFTNLRYGIGSALSIILMLILLIVTVVYLRVLRATAGAEEV"
     gene            4253..5122
                     /locus_tag="PLM74_02245"
     CDS             4253..5122
                     /locus_tag="PLM74_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HON41937.1"
                     /translation="MRPRRAIQQILLYLAVGLVVLVVLVPYAWMISGSFKETLELQSS
                     DVTVARLRPTWIPRRPTLRNYREVNSSVPMLSYFRHSILISLGTMAVATVLALLAAYA
                     LSRFNFRGREAYTVSVLATQMLPGILFLIPYYLIFTTISRKTGIPLRDTYWGMIFTYT
                     SFALPFSIVMLRSYMNGIPLEIDEQAMIDGCTRTRALWSVIVPLATPGIAAVAIYAFI
                     MAWNEVLFASVLTGRVTRTVSVGIMDYITATSSNWGGMMAACIVISIPVLILFTAMQR
                     YIVEGLVAGATKG"
     gene            5273..6538
                     /locus_tag="PLM74_02250"
     CDS             5273..6538
                     /locus_tag="PLM74_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HON41938.1"
                     /translation="MRRFLRAVVMLVFLLGVLTSMTSGAAREITFWVMPNAPQDTHTA
                     WLDSRIEEFRKETGITVHYEIVGWGDAWNRLVAAITSGEGADVVQVGTTWNPQLAAMK
                     GLAKLDTAEYGGTKAFMPANYESATYKGAAYGVPWFAETRCLFYNTDHFAQAKVQPPK
                     TYAELVEVGKALVKARGAGTAIAIAGNQAWDLLHNWAIVLWAFGGDLVSPDLKKATFN
                     GPEGVEAMNWYVDLVRTGLASKACAEYNQPNADAAFINGNVSMAFMGPWNVADIIHDN
                     PSLKYDIVEPPAGPKGRAAFSGGSNLAILAASKKQADSKAWVMYLIRKDTLISYTKDL
                     THMLPATKEAFADPFFSTGVNRAFKAALEYATAYPPLGVWGSIENDITGEFKAIFTDY
                     VTGQLGPNGVQQHLDIAAQRVDTSVKNER"
     gene            6641..9043
                     /locus_tag="PLM74_02255"
     CDS             6641..9043
                     /locus_tag="PLM74_02255"
                     /inference="COORDINATES: protein motif:HMM:NF023636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl hydrolase"
                     /protein_id="HON41939.1"
                     /translation="MPHYTSDDKLGSGVPLGGIGAGKIEIFPDGTCANFTHQNNWDNP
                     SGTAADPSAGPGAGPGAGPGAGPGVIDARVGHHFAIWAHRRVSADAGVGLIPGTGDGA
                     PVARLLQTVDVAGLARVERIEYEGSYPFARLDYVDSSLPAIVRLEAFSPIVPGDSRQS
                     AMPVALFVFHVTNTDPIAAYDVAVMAAARNSVGSWNVGRFNRTVTDENVAGVLFCVDS
                     PLPIDSRAGTVCLATTRAAGEVSCEPAWNLKTAEPFSLTLANEDLAPWNAFAESGRLS
                     LDLGLGLAPTPVPVPVPGPGRPSMASSDVVSGEGVELGAAVCVSTTLGPGETRSIPFA
                     MSWHMPQTHFGHVYERDFDDAWSVAVAALHNIGAIREGAGLWESCLAATRTPDPPPTP
                     ITAVSAVSAPRPALPAWLDDALRNNLYVLSSGSWWDRDGRFALFEASRTCQLMSTVDV
                     LYYASIPVSWCYPDLQESCISQIAAAQRNDGYVPHDLGRGRIDYPSDGTTSPPRWKDL
                     CPKFVLMVYRDCLWWGGKRLLDRMYSAAKRAMRWEMRTDANGDGLPDNEGADQTFDNW
                     SFHGTNSYTSSIYLASLLACERMAEIMADTRFAHECRAAFVRGQGSFEAQLWNGRFYS
                     ATAADPACTVGQLNGQWYAHLLGLGYIVPPLHVASAVRAMLDINGSASEYGAVNSVFP
                     DGRADRTNPHSGNIWPGEAYALASLAIYEGFVEDGLDLARKTWSNITDHIQNPWDQPD
                     VIDSLTGEYGFGDHYMRNMGIWALAFALAKHDCHAERALCALSQSRLTSVQGVILGRK
                     AF"
     gene            9763..11049
                     /locus_tag="PLM74_02260"
     CDS             9763..11049
                     /locus_tag="PLM74_02260"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HON41940.1"
                     /translation="MAATACGRQRPSHLRDDRINYAIFFADSFGYPLAFSLISPTTIM
                     PFLLTKLSASNLAIGMLPALASLGAFLPGLLSAAYLQKIPVKKHMLVVFGMVERTFVL
                     SMAAGLMLWAVSRPHVAVIWILASWTIMSLVSGFILPAFHSMMAKCIRPEVRGGMIGW
                     SGAAAGIGGVFAAQAAGVVLSHMPFPVNYSILFVAAFVILSATILPFLLTREPADQTP
                     EEIRSVAQYVRDAMKTVKSNKGYSFAIYALSVMSFALMAVTFYSTHAVRNLGAGEIEV
                     ARFAAITVGASVISFPVLGRIADRHGHKRVLQVAAMGFAIAAALALASNSVSGIRATL
                     VFASLGSTGLAVSQNVIWAEFAPSHADVPMYTAVSLLFLMPFRVVAPLMAGWIADVWG
                     FPTMFWAALTAGVAAFAILYTAVPEPRSMHCAAVDT"
     gene            complement(11164..11820)
                     /locus_tag="PLM74_02265"
     CDS             complement(11164..11820)
                     /locus_tag="PLM74_02265"
                     /inference="COORDINATES: protein motif:HMM:NF024741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HON41941.1"
                     /translation="MLGLVWKDFYINRSQLVFPAIVLCAIALLAAVGRSSPTTVDYAV
                     PLLFTYSGLMVPFFALNSAMLEEKNRTLAFLRSLPMRADAIVASKFVAPLLVGVLWVG
                     ASALAAIIMQAGMGEHTVSIMSGMLLVCATLPIAAIELVVFFRFGASAARTALIVVVG
                     TVYLVPLLAQGLVAKVKPIVVAMMDPEPVHAVLFAGGSLLLYFAGMLIAQAIFRRREM
                     "
     gene            complement(11822..12553)
                     /locus_tag="PLM74_02270"
     CDS             complement(11822..12553)
                     /locus_tag="PLM74_02270"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON41942.1"
                     /translation="MSENVLMLDGVCKSYRGFELRNVSFVLPRGYIMGLVGPNGAGKT
                     TTIRLIMDLARPDSGRIAVLGQQMAANQIELKRRIGYVPENHEFYSDMTVNWTAQFVG
                     RQYPTWNEARFRELLQRYDVDPRKKIGALSKGTKAKLAMALALAHHPELLILDEPTSG
                     LDPVVRRELLSDLLEFVADGDRSVLFSTHITSDLERVADYITFIVNGRVAASDEREVM
                     LERARASNPTASIEDLLWTYAKGGH"
     gene            complement(12546..12932)
                     /locus_tag="PLM74_02275"
     CDS             complement(12546..12932)
                     /locus_tag="PLM74_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HON41943.1"
                     /translation="MDITVSANNPVPLYQQIVHQVRAKVLTGELKAGDPLPSIRQLAS
                     QLIASVITTKRAYSELEAEGLIVTRAGLGTFVADLDPETIVELKKEVVMEHLEEGVRV
                     ARQLEFVDEEINRLFRQALDGSEKRV"
BASE COUNT         2716 a         4083 c         3688 g         2616 t
ORIGIN      
        1 gtagcaatcg cgaccatcgg gataatgtcc ctgattctcc tgggctagcc gtgacgttct
       61 gatgctcagg tccgacgcga ctaccgagac tccaaaaggt gcgggggctt cgcagggcta
      121 gtagtgccca agaagtcccc ggttcttcgc aatgcgctcg caccacgaga acaccgctat
      181 gccggcagcc aggtaggccg cgcccactgc gacggcggtg agtatgtcgc ccagaggtag
      241 gcgccacagg cgaacaccgt ctaccatcga cctgtatatg aggtcatttc caagggcaag
      301 cggcagatac ttcactcccg gtactcgaat agggatcacc agcaggccaa tgaatacgaa
      361 ctgaattatc tggaacaacg cctgtatcct cttgaatacc agagccagcc cgcccagggc
      421 aaagccgacg ccgtaggctc ccagcaacgt aatgaacagc aggggaatca gggtaaccag
      481 gtccactttg agccacctgc cggttgtggc catcatcgcc atgagcagaa gcatgatggg
      541 gatgaagctg aacacgaagt cggacatgag cgttgcggca cccacccact tgaacccgca
      601 tggggccagg taaagctgtt cgagcgtgcc ggtctgcgct tcgtttatga gattccatga
      661 gaggttcgag aaggcgtaga tggtaaaggt ccacacgaag aatccaacaa ttgttccctc
      721 aaaagagccg tcgagagcac ccgtctttac gtagccggac acactctgaa gccccgcgaa
      781 gatcaatatg aagatcatgt atatagacac caggctcgat acggagttga ctatgtaccg
      841 tttcatcatc gtccaatccc gcctgaaaac agcggcaaga acactcccca tcatcatgaa
      901 cgcgccctct cctctctcac gatctccaga tacaccttct ccagatcgac ctcttcgtgc
      961 gtgatctcgt ctatgatcgc atccgcgccg cgcaagatgt ccatgaggcg gtagatatca
     1021 ccgtcttcgc tcaatgagac cttgacccta gagtccgcat ccccgtctgt cacctgcacg
     1081 cttgcgaact cctgagccag tcgctccttg agcccctgcg gggcaggccc acgcaggcct
     1141 atccggtacg ccttcgtccg gaagagactc atcaagtggc ttaccttatc atcaacgact
     1201 acccgcccgg aggacattat gaccacgcgc ccgcatacat cctgaatgac gtccatgtcg
     1261 tggctgctca ctacgaaagt acgctcctcg tcgcgagcaa gctgcttcag gacgctccga
     1321 agctccagcg aggtttcgac atcgaggccc agggtgggct cgtccagaaa gactagtgga
     1381 gtgcgtcggg ccagggcgca cacgacgctg gccttctgct tcattccctg cgagagtttc
     1441 ctgacttctg tatggcgctt gtcctccagt gcgaatagct tgagcagatg gccgaaatac
     1501 tctcggtctt cgcgagacct gacaccatgg agtgctgcga agaacactac attctcccac
     1561 acgctcattc tccagtacag attccggctt ccctcaagaa ctgcgctcat tttccccaat
     1621 atccgcgggt aagctgcctg gatatcctgt cccaacagcg cgatccgccc cgaatcaggg
     1681 cgcatcagcc cgaggatgca cttgattgac gtggtcttcc ccgccccgtt tggtcccaaa
     1741 agccctagga cctcgccgcg cttcacgtcg aagcaaacac cgtcgacggc tcgaactgtc
     1801 accccgcctc tgtcacgaaa gaccttggtc agcgattgca cttgcagcgc agtatcaagt
     1861 gtgctcaagc gtcgcgcacc cccaaacaat attgtcgctg ccgcatctcc ttctcgggaa
     1921 tcgggccgcc cgacgaggca gcgcttccac acatcaatgc tagacgctag catcagcatt
     1981 gtcaatgtct ctgttgctaa tatcaactcc aatgtgaatt ctattaatga cgcatagcac
     2041 actccggagt gggcatacgc tgtacgcata gccggcgtca cccgcagaaa aaacttgtga
     2101 cgccagaagg aatctcatcc gacatgtcga agtgacttac cgctcggttg tgacatcgat
     2161 gtcatgcgac cagcccatca agaatacgct gcagtacact caaccggagg tgccgtcact
     2221 tgcacagtac catatatgac gtagcggaac gcgcaggcgt atccgcgaag accgtatcga
     2281 gggtcatcaa caactcacct ctagttgcgc cggatacgag gcgcagggtg ctggctgcca
     2341 tcgctgagct ggacttccac cccgattcgg cagccaaggg cctccgccag cgaagccgga
     2401 aatcggtggg gttcgtgatt ccctacggca gcgatttcgt ctttcaggac cagggcatgt
     2461 ggctgcagac caacggagcg cacaaggcat tggctgagcg aggctatgat ctggtactgg
     2521 ccgtgccgca gactccagaa ctcgtcctgg ccgagttgaa caggctcacg cgcaaccgca
     2581 ctgtggacgg cgtcatactc tacgctatgg aaggcgccga cgctctactc cgcgagttcg
     2641 atcgactcaa catcagatat atgtcgctgt cgatgtgcta ccccgggcag aagaacaact
     2701 tcgtcgacat ggactccccg tacaccgctc acctggcaac caagtacatg atcgaccagg
     2761 gcgcccggcg cataggcctg atttccgaac ctgaacggtt tttctactca gccaaggcca
     2821 gttctgagcc gggttaccgc cttgcccacg aacaggccgg tctgccggtg gatgaatcgc
     2881 tgatcctcgc cggcgactac tctatcgagt caggctacga aatggcaaag cgattgctca
     2941 catcgaggcc ggcagtggac ggggtcatat gcatgagcga tccgatggcg ttgggcgctc
     3001 tgcgggccgt gagggaacag ggcctggtcc ccggacgcga catcgatgtc atcgcaggcg
     3061 atgacttccc cacaacacgc gcgatgtggc cggggcttac gtccatcaga acgcccctct
     3121 acgagcaggg ttaccaggca ggcgtgatga tagtggacta catacaatca ggcaaggaga
     3181 tcccgggcaa gctgcttagg ggagagctga tcgttcgcga gtcggtgcgg tcccgcaatg
     3241 ggataaggga gggtctgggt tgatgctatg gcagtcaggc tccacgctgc taacgcagtc
     3301 ggataggcgg tcggcgggca aggccggcgc cctctccgga tggtccaggc gccaacaccg
     3361 aacggcctac ttaatgatcc tgccggcggt agtcgcaatg ctgctggtcc actacatgcc
     3421 gatgctgtgg ggtttctaca tcagtctaca cgatgtcaac cttttcacca tggctgactg
     3481 gatgaatgcc ccgttcatcg ggctgcgcaa cttcgtagac gcgttcgatc cgtctactac
     3541 cacaggtgcc cgcgcgctga gggcattgct caatatcacg gtgtattcag cggtgaccgt
     3601 cagtgtgggg tacctgattg ggcttgcagt cgctcttctg cttaaccagc agttctgggg
     3661 cagaacgttt gtgagaggac tgatccttct tccatacata acgccggact cagtagccta
     3721 caaggtctgg acattcatct ttcagtccag gatcggaata ctcaaccgct acctgataca
     3781 atggggcctg gtgtccgagc cccctatctg gctcataggt ccacccgcgc tatgggcggt
     3841 ggtcgcagcg agcgtctgga aaggctggcc gttctccgcc ctcatactcc tcgccgcact
     3901 gcagaagatc ccgcaggacc tttacgaggc tgcccgcgtc gacggcgcca gtatctggca
     3961 acagttcgcg tggattacca tgccacatct tcagtcggtc acggtgacac tgctcctgat
     4021 gtcgatacta tggaacttca acgccttcaa ccagttctac gtgatgctcg ggcgcgatcc
     4081 cggagtcgct gcagaggttc cgtctaccct gatactgaga gaggcgttca caaaccttag
     4141 gtacggcatc ggttctgccc tgtccataat cctcatgctg attcttctga tcgtaacagt
     4201 agtttacctg cgcgttctgc gggctaccgc aggcgcggag gaggtgtaga gtatgcgtcc
     4261 ccggcgggct atccagcaga ttctgctata cctggcagta gggctcgtgg tgctggtagt
     4321 gctggttcca tacgcctgga tgatctccgg atcgttcaag gagacactgg agttgcagtc
     4381 ttcagacgtc acagtcgccc ggctcaggcc gacgtggatt cccaggcgcc cgacactgcg
     4441 caactacagg gaagtgaata gctccgtccc gatgctctcg tatttccgac actcgatact
     4501 catcagccta ggcaccatgg cggtcgcaac ggtgctggca ttgctggcgg cgtacgcttt
     4561 gtcgaggttc aacttccgcg gacgtgaagc atacacggtc tcggttctgg cgacacaaat
     4621 gcttcccggc atactgttcc tcataccgta ctacctgata ttcaccacaa tctcgcgcaa
     4681 aaccggcatt ccgctccgcg atacctattg gggtatgatc ttcacgtaca cgtcctttgc
     4741 gctgccattc tccatcgtta tgctgagaag ctacatgaac ggcataccac tggaaatcga
     4801 cgagcaggcc atgatagacg gatgcacccg cacccgcgca ctctggagcg taatagtccc
     4861 gctcgctact cccggcattg ccgccgtggc aatctacgcg tttatcatgg catggaatga
     4921 ggtgttgttc gcgtcagtgt tgactggccg agtcacgcga actgtgtcgg tgggcattat
     4981 ggactacatc acagcgacca gctctaactg gggcgggatg atggccgcat gcatcgtcat
     5041 aagcatcccg gtcctgatcc tcttcacggc gatgcagcga tacatagtgg aaggattggt
     5101 ggccggggcg accaaaggct gagatccagg aatccgactg caaggacgtc cttggaggtg
     5161 atggcgccga catacctgcg gaaagctcag catggtcttg cgtttgcgtt tgcgtctgtg
     5221 tctgtgtccg cttcgatggc gatcacaaag tccaaaaagg aggacagttc agatgagacg
     5281 gtttctacgc gcggttgtga tgctcgtttt tctgttaggt gttttgacat cgatgacatc
     5341 cggggcggct cgagaaatca cgttctgggt gatgcccaac gcgccgcagg atacccacac
     5401 ggcatggcta gactccagaa ttgaggagtt ccggaaggaa actggtatca ccgtccacta
     5461 cgaaatcgtt ggatggggag acgcatggaa cagactagtg gccgcgatca ccagcggaga
     5521 aggcgccgac gttgttcagg tcggaaccac gtggaaccca cagctcgcgg cgatgaaggg
     5581 gcttgcgaag ctcgacacag ccgagtacgg tggcacgaag gcattcatgc ctgccaacta
     5641 cgagtccgct acctacaagg gtgcagcata tggcgtgcca tggttcgccg agactcgctg
     5701 cctgttctac aacaccgacc acttcgccca ggccaaggta cagcctccca agacctacgc
     5761 cgagctggta gaggtgggca aggcgctggt caaggcgcgc ggcgccggca cagccatcgc
     5821 tatcgccggc aaccaagcat gggacctcct ccacaactgg gcgatcgtcc tttgggcttt
     5881 cggaggcgat ctggtaagcc cggacttgaa gaaggcaacc ttcaacgggc ctgaaggtgt
     5941 tgaggccatg aactggtatg ttgaccttgt acgtaccggc ctggcatcta aagcatgcgc
     6001 tgagtacaac cagcccaacg cagatgcggc cttcatcaac ggaaacgtgt ccatggcgtt
     6061 catgggaccg tggaatgtgg ccgacatcat acacgacaac ccgtctctca agtacgacat
     6121 cgtcgaaccg ccggcaggtc caaaagggcg ggcggccttc tcaggcggaa gcaaccttgc
     6181 gattctcgct gcaagcaaga agcaggcaga ctctaaggcc tgggtcatgt acctgatccg
     6241 gaaagacact ctcatctcct acactaagga cctgacccac atgctgccgg ctactaagga
     6301 agccttcgca gacccattct tcagcaccgg cgtcaaccgg gccttcaagg ccgctctgga
     6361 gtatgcgacg gcatatccgc cgcttggcgt atgggggtcc atcgagaatg acattaccgg
     6421 cgagttcaag gcgatcttca ccgattacgt cacaggccag ctgggcccga acggagtgca
     6481 gcagcatctc gacatcgctg cgcaacgcgt agacacatct gtgaaaaacg aaaggtaatc
     6541 gagacggagc gcgggccggc catgttcccg gcatgtgtgg gcatggccgg cccgcgccta
     6601 gccccaatcc gtagcgacca cgaaacggag gacgacagag ttgcctcact acacgtcaga
     6661 tgacaagctg ggctccgggg ttccccttgg cgggatcggg gcgggaaaga tcgagatatt
     6721 cccggacgga acttgcgcca acttcacgca ccagaacaac tgggacaacc cgtcaggtac
     6781 tgccgccgat ccctctgccg ggcccggcgc tggccccggc gctggcccgg gcgctggccc
     6841 cggcgtcatc gatgctcgag tgggccatca cttcgccatc tgggcacaca ggcgtgtgag
     6901 cgcagatgca ggcgtgggcc tgatcccagg cacaggcgac ggcgcacctg tcgcacgtct
     6961 gcttcaaacc gtagacgtcg ccgggcttgc gcgcgtggag cgaatcgagt acgaaggatc
     7021 gtacccattc gcgaggctcg actacgttga ctcgagcctg cccgcgatag tgcgtcttga
     7081 ggccttttcg ccgattgtgc ccggcgattc gaggcaatcg gctatgcctg tggcgctgtt
     7141 cgtcttccac gtgactaata ccgacccgat tgcagcgtat gatgtagccg tcatggcggc
     7201 tgcgagaaac agtgtaggct cgtggaatgt cggccgcttc aaccggacgg tgactgacga
     7261 aaatgtagcc ggagtgcttt tctgtgtgga ctcccccctc cccatcgact cccgcgccgg
     7321 cacggtctgc ctcgcgacaa ccagagctgc aggagaggtt agctgcgagc cggcatggaa
     7381 tctcaagacc gccgagccct tcagcctcac cctcgccaac gaagacctgg ccccatggaa
     7441 cgccttcgcc gagagcggca ggttgtcgct ggacctcggc ctcggcctcg cccccacgcc
     7501 cgtgccagtg cccgtgcccg ggccgggccg gccctccatg gcgtcttctg atgtagtcag
     7561 cggcgaaggt gtcgaactgg gggcggcggt gtgcgtgtcg accaccctgg gccccggcga
     7621 aactcgatcc atcccgttcg ccatgtcctg gcacatgccg cagacgcact tcggccacgt
     7681 atatgagcgt gacttcgacg acgcgtggag cgtcgccgtg gccgcactac acaatatagg
     7741 cgctatccga gaaggcgcag gactgtggga gtcgtgcctg gctgctacca gaactcctga
     7801 tccgcccccc acacccatca cggcggtctc ggccgtctct gccccgcgtc ccgcgctgcc
     7861 cgcgtggctc gacgacgccc tgcgaaacaa cctctacgtg ctgtcctcag gaagctggtg
     7921 ggatcgggac ggacggttcg cactcttcga ggcgtcaagg acatgccagc tcatgagcac
     7981 cgtggacgtc ctgtactatg cgtccattcc tgtatcatgg tgctaccccg acctgcaaga
     8041 gtcttgcatc agccagatag ccgcagccca gcgcaacgac gggtatgtcc cccatgacct
     8101 ggggcgtggc aggatcgact acccaagcga cggcaccaca tcccctccgc gatggaagga
     8161 cctctgcccc aagttcgtcc tgatggtgta ccgtgactgc ctctggtggg gtggaaaacg
     8221 cctacttgac aggatgtata gcgcggccaa gcgcgcaatg cgctgggaga tgcgaaccga
     8281 cgccaacggc gacggcctcc ccgacaacga aggcgccgac cagaccttcg acaactggag
     8341 ctttcacggc acaaactcct acacgtccag catctacctt gcttcgctcc tcgcctgcga
     8401 acggatggcg gagatcatgg ccgatacccg gttcgcgcat gagtgccgcg ccgcattcgt
     8461 ccgggggcaa gggtcattcg aagcacagct gtggaatggc cgattctact cggcaaccgc
     8521 ggctgatccg gcctgcactg tcggccagct caatgggcaa tggtacgcac acttgctggg
     8581 gctgggctac atcgtcccgc cacttcatgt cgcgtcggca gtgcgcgcaa tgcttgacat
     8641 caacggctca gcctccgagt atggagcagt caactcagtc ttcccggacg ggcgtgccga
     8701 caggaccaac ccacactctg ggaatatatg gcccggcgag gcgtatgccc tggcctcgct
     8761 ggcaatatac gaagggtttg ttgaggacgg gttggatctg gcgcgcaaaa cgtggtccaa
     8821 catcaccgac cacatccaaa acccgtggga ccagccggac gtgatcgatt cgctcaccgg
     8881 cgagtacggg ttcggcgatc actacatgcg caacatgggc atatgggcgc tagccttcgc
     8941 gctcgcgaag cacgactgcc acgcagaacg ggccctttgc gccctctcgc agtcgcggct
     9001 gacatcagta cagggggtca tcctcgggcg aaaggccttt taactggtct agctgagatc
     9061 ggtgtgggct acaccggctg gagctgggat gttgtcaatt attaccgata ctttctgaga
     9121 tacccgcaat ttttgagtac atcgatttcg gaggaccgcc gaaccgcctc caaactgcga
     9181 aaatcggggg gtatccgacg aatcagagca ccgaaccaga caatcgagct gacataatat
     9241 cctataatct ggaccgatgt ggaaacagca tgcagacgat ggcccgtatt gccgcctatc
     9301 taccggataa tgggcgatag ctggccccga gagcgcccct cgcagcaccg attcgccccc
     9361 ctgggccccc ggcccaaatc gatgtactca aaagttgcgt ttttcgccga aactattgtc
     9421 aagaagtgac ggtatctctg ggcagatacc cccgggggta tatcgccttg ttgtaacgat
     9481 tgcctttccg accccgggcc tacccccaac gcctatcccc gggtcccctc ctgccccagc
     9541 ccccgctggc cgccttcact gtgattctgg cggaccgaag agcagcaatc gccagacgca
     9601 gactcggcgt acgctcacgg cccgcaggcc tggcgccgca cgcaaaatct cggtagaatg
     9661 gtcgtatggc gccagtgtca acgctagccc gatcccacgg ccgggcgcta gcagagcact
     9721 gcactttgga tagcaagcgg accggaaatg gaggaactag ttgtggcagc aactgcttgc
     9781 ggtcgccagc ggcccagcca tcttcgggat gaccgaatca actatgcgat tttcttcgcg
     9841 gactcattcg gctacccgct ggcgttctcg ctcatatcgc caacaaccat catgccgttc
     9901 ctcttgacca agttgagcgc ctccaacctc gcaatcggga tgctgccggc gctcgcgtca
     9961 cttggggcat tcctgccagg cctgctatct gcggcttacc ttcagaagat tccggtgaag
    10021 aagcacatgt tggtagtatt cggcatggta gagcgaacct ttgtactctc catggcagcg
    10081 gggctgatgc tgtgggcagt cagccggccg catgttgctg tgatatggat cctcgcttcc
    10141 tggacgatca tgagcctggt ctccgggttc atcctccccg ctttccattc catgatggcc
    10201 aagtgcatcc gacccgaggt gcgtggggga atgatcggct ggagcggcgc cgctgccggg
    10261 atcggaggcg tattcgcagc tcaggccgct ggcgttgttc tttcgcacat gccgtttcca
    10321 gtcaattact ccatactgtt cgtggcggcg ttcgtgatcc tctcagccac gatactgccg
    10381 ttcctcctta ctcgtgagcc cgctgaccag accccagaag agatccgctc cgtggcacag
    10441 tacgtacgcg acgcgatgaa gacggtgaaa tcgaacaagg gatactcatt cgccatatat
    10501 gcgttgtcgg tgatgtcatt cgcactcatg gccgtgacgt tctacagcac gcatgcagta
    10561 cgcaacctgg gcgccggaga gatagaagta gctcgcttcg ctgcaatcac agtcggagcg
    10621 agcgtcatca gcttccccgt gctgggcagg atcgccgacc gtcacggcca caaacgagtc
    10681 ctgcaggtag cagcaatggg tttcgctatt gcggccgcgc ttgcactggc atcgaactca
    10741 gtaagcggca tccgtgcaac gctagtgttc gcaagcctgg gttccactgg actcgcggtg
    10801 agccagaacg tgatatgggc cgaattcgcc ccatcacacg ccgacgtacc aatgtacacg
    10861 gcggtttcac tgctcttcct aatgccattc cgcgtagtgg cccccctcat ggccggatgg
    10921 atagccgatg tgtggggctt tcccaccatg ttctgggcag ccctcactgc gggagtcgcg
    10981 gccttcgcta tcctctatac tgcggtacct gagcctcgca gtatgcactg cgccgcagta
    11041 gatacctgat ctgccccgac ccccccatcc gcctccaagg ccctccgcca gagatccgcg
    11101 cgcccctgcg cgggcggtcg ttcattcgtc ggcgccaggc cgggagctcc ccaagacatg
    11161 ctgctacatc tcgcgcctcc tgaatatcgc ctgcgcaatc agcatgcccg cgaagtagag
    11221 cagcagcgag ccgccggcga aaagcacggc atggaccggc tcgggatcca tcatggccac
    11281 gacgataggt ttcaccttcg caaccaagcc ctgtgcgagc aacggcacta ggtacaccgt
    11341 tcccaccacg acgatcaggg ccgtacgcgc ggcgctggct ccaaacctga agaacacgac
    11401 aagctcgatc gctgctatgg gcaatgtggc acagaccaga agcatgcccg acatgatgga
    11461 cacggtatgt tcgcccatgc ccgcttgcat gatgatcgcc gccagagcag acgccccgac
    11521 ccacagcacg cccacgagta acggggccac gaacttcgag gcgactatgg cgtcagccct
    11581 catgggaagc gaccggagaa atgcgagcgt ccggttcttc tcctcgagca tcgccgagtt
    11641 gagcgcgaag aagggtacca tcaggccgga gtatgtgaac agcagaggga ctgcgtagtc
    11701 caccgtagtc ggtgacgatc tccccacggc cgccagcagg gcgatggcgc acagcacaat
    11761 cgccgggaac acaagctgtg accggttgat gtagaagtcc ttccatacaa gaccgagcat
    11821 atcagtggcc tcccttcgcg tatgtccaga gcagatcctc gatcgacgcc gttgggtttg
    11881 aggcgcgggc tcgctccagc attacctctc gctcgtcgct cgctgctacg cgcccattca
    11941 cgatgaaggt aatgtagtcg gccacccgct caagatcgga cgtgatgtgc gtagagaaca
    12001 acaccgaacg gtcgccgtct gcaacaaact ccaacaggtc agacagaagt tcgcgtctga
    12061 cgacaggatc caggccgctt gtgggttcat ccaggatcag cagctcgggg tgatgcgcca
    12121 gagccagagc catcgccagt ttagccttgg tgcctttgga caaggctccg atcttctttc
    12181 gcggatccac atcgtatctt tgcagcaact cccggaatct cgcctcgttc caggtcggat
    12241 attggcgccc gacgaactga gcagtccagt tcaccgtcat gtcagagtag aactcgtggt
    12301 tttcgggcac gtacccgatc ctccgcttca gctctatctg attcgccgcc atctgctgcc
    12361 cgagcactgc aatacgcccg gaatcgggcc gcgctaggtc cattatcagg cgaatggttg
    12421 tcgtcttccc ggcgccattc ggacccacaa ggcccattat gtagccgcgg ggaagaacga
    12481 acgacacgtt gcgtagctcg aatccccggt agctcttgca tacgccatca agcatcagca
    12541 cgttttcaga cacgcttttc actcccatcc agtgcctgtc ggaacaggcg attgatctcc
    12601 tcatccacaa actcaagctg cctggccacg cgaaccccct cttccaagtg ctccatcacc
    12661 acttccttct tgagctcgac gatggtttcc ggatcgaggt cagctacaaa cgtgcccaaa
    12721 cccgctctcg ttactatcag cccttcggcc tccaactccg aatacgcccg cttcgtggtg
    12781 atcacgctag caatcagctg gcttgccaat tgcctaatgg acggaagagg atcaccggct
    12841 ttcaactctc ccgtcagcac cttagccctc acctgatgca cgatctgctg gtacagggga
    12901 accgggttat tcgccgacac ggtgatgtcc ataccaaacc ccgccactgt gtatattgtc
    12961 tatatacact atactcacct gtcgccagta tgtcaacatg ggtattccat gacgcgggtg
    13021 gttccatcac gcgggtggtc tcatcgcgcg ggtggtccca tcgagtgtcc caccgcgccg
    13081 gcccggcccg ctccacgcgg ggg
//
LOCUS       DAOTAC010000029        12947 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_4063_length_12947_cov_4.819811, whole genome shotgun sequence.
ACCESSION   DAOTAC010000029 DAOTAC010000000
VERSION     DAOTAC010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 12947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12947
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4063_length_12947_cov_4.819811"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..911)
                     /locus_tag="PLM74_02280"
     CDS             complement(<1..911)
                     /locus_tag="PLM74_02280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HON41944.1"
                     /translation="MGGTTAMVFGMIGGLGLFLYGMTLTGDGLRRAAGEKMKHVLEVL
                     TSTPLKGVIVGAVVTAVMQSSSATTVMLVSFVNAGLMTLRQTVGVIMGANIGTTVTAQ
                     LIAFNLADYAYPAIGIGFGMIMLARRKVWRSVGQVILGFGILFLGLTTMSAAVAPLRG
                     SAVFAKAMRTLASSPVLALLTGLVMTLVIQSSAATIGMLLAVAIASPDLISIEVAIPI
                     LLGDNIGTCITALLASIGTNHTARRTAMVHLLFNVFGAIIFMILRIPFAWLVTAVSPV
                     GGIARQIANAHTMFNVLNTVVWLPGAG"
     gene            complement(921..2174)
                     /locus_tag="PLM74_02285"
     CDS             complement(921..2174)
                     /locus_tag="PLM74_02285"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HON41945.1"
                     /translation="MPRDRHVLHDAYALAIISAAALAVAYTIRWEVPLASAVVFVSLL
                     WASSLSVIVLRTTVVVMMLPVMMACIIKYDAGTSAILAMLGGISIQEFRIIRENNSPY
                     LTGLKLVLNRAITALCAICAWLAFHAIWPGRDIALGNFRFVAAAGVSGLVWVIVSGII
                     VNFQILLSKPVFRFRVDPSIVLNSVSSVVPSIVMGLMAAAVYEYGGLVAFALAELFFI
                     SNKNYTQRALVQKENAEQINCALARIIDSKDHYTAGHSERVAQLASYLAEACNLSRGD
                     VERIAYIARLHDLGKVNIPQEILTKPGELTESEFAEVKKHPGWGAELIRGMDKIYNDR
                     DYRAILDHHERYDGTGYPFGRKGKDISLWGRILAVCDAYDAMTSERVYRAAMTKDAAR
                     EELTRNAGTQFDPRLVQLFVSKVQG"
     gene            complement(2508..3227)
                     /locus_tag="PLM74_02290"
     CDS             complement(2508..3227)
                     /locus_tag="PLM74_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41946.1"
                     /translation="MVSRIVFRNASQRLAWMIGGVVMGGEREAKLSRAEEHLCQLPGV
                     ARARIVQGAEGDEIHVMVSGHLDQDEIKRMKKDIETIYLLDVGERVDYRKVSIAQVSA
                     AEEDVGRCSGSRPVLSRISLEYGPSRTVTAEIHLDYMGQSHVGKASGCSDGDCICDIV
                     KIATVDALESMMGGGYRLQASCELSGDRVVSEITVTCVSTGQRTRYIGAAYRKDDVPT
                     SVARSVLQALNRQLQRLAMAE"
     gene            3305..4222
                     /locus_tag="PLM74_02295"
     CDS             3305..4222
                     /locus_tag="PLM74_02295"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HON41947.1"
                     /translation="MSTDSSSRKRSVAALVFTTTMWGLSFISTKVLLRHMAPVQVAFL
                     RHAIASVATWIAVAVARPDIRVALRDLPSMLTAAIVGIPIYYYFENTGLLYLSAATAS
                     IITASTPAMTAAMESIFYRRRLPIAGWAGILVSAGGVALVVQSGAAQAAAASVAGRSD
                     FLLGAAMLLVSAFAWSLYTIFNRPNVASYGSLTTNAYQITFATAVLYVATLRNGIPWR
                     EALSSPQVLLNLGYLGIFCSAIGYLLYLFALRSLGPTVVSAFINLVPVFGVAGSALIL
                     GERVTLVQLVGGAIVVLGIFAVERMQGGA"
     gene            4222..5115
                     /locus_tag="PLM74_02300"
     CDS             4222..5115
                     /locus_tag="PLM74_02300"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HON41948.1"
                     /translation="MRRYTLLACDIDGTLLRSDGTASSRTLKALRLAETSGAMVIIAT
                     GRRIASTTPIARRLGIHSPCVAHCGAVVYDPSNESILMARQIPRQIALRACRLAGSVG
                     ADVAVHESVYSGRSIFVTTSKELEEAAEHWPFMTPYYRLVDGFEQACLADPVQLCLMA
                     DADIIPGLKRRLACDMPETLSIVDYGIVENGKHMLDIFAHGVDKAVGLTFVADLYGIG
                     KPETVGFGDSVNDIELICHAGLGVAMGNAPDDIRRIADLVAPSNDDDGVAAVIEELAA
                     AGLLGRGAVPSCIKHASSRLH"
     gene            complement(5096..5965)
                     /locus_tag="PLM74_02305"
     CDS             complement(5096..5965)
                     /locus_tag="PLM74_02305"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HON41949.1"
                     /translation="MLAGRCRRCIVGIVMAAIVVAVMAGLRLAPGLGFQGQSAHAPAA
                     HFLSKENAGSADANDLYVIGRCTTQLDPDQRGRNDNISIAAEKLNGTVVGPGQEFSFN
                     EVVGERSSELGYGPARVIRDGRSEPGIAGGICQLSSTLYNAALLAGMEITERRPHSRP
                     VPYLPAGLDATVSFGAADLRWVNVWATPVTVRARIGADSVSVWIEGQSAVPDVRLYTE
                     VIEVIAPRPAAPPDPTGRAKSPPLPGADAVASAGAPGYRVRVWAETEAPEGGRQRLLV
                     SEDLYEPVNAVAN"
     gene            complement(5996..10477)
                     /locus_tag="PLM74_02310"
     CDS             complement(5996..10477)
                     /locus_tag="PLM74_02310"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HON41950.1"
                     /translation="MGTNNRLARIIVPCIAVVLLMAACLLAAEPAMAARQAYAWLFAT
                     ARTYLPGEQAELGVRGRGVSQLYLDIYRFDGARHFSESGPDAMWSMNPARLPGRSLVR
                     TVSVRIPNPGGNLEASVKLDPLPPGAYLAVTRSKQLRQQDTVWFTVSGIGLISKQSAG
                     RLLVYAVDLRTGEPAAGVPVQVALMGIGGDDQLASATAASRTVTGVTDDSGLFSSPLS
                     QPAASAMVTGGRGDEFAVLYSSFWFDARQQKVYVYTDRPIYRPDNTVYFKGIARTMSD
                     PGYTIPAGQAVKVEIRDPRDNLLSKQELTTNDWGSFYGEFTLGSEPPLGVYSVVATIG
                     GEQHWSSFSVAEYRKPEWSVDVKFSRDRYIAGDTLQAICEASYYFGAPVANAQVEYRV
                     YRQRIGSFAAAASGADGEYGYDDYGDYDGYGDYYEDFVMSGEASTDSQGRALISFEAP
                     RADGRNYKYIMAVDVIDATNKRASGRNSALVAMGSFDMRVSTSAYLVSPGDRFQVRVK
                     TVALDGSSVGRKLELVMMKRTYDKTGYTDKPVATRAVETSSSGDAMVELTATEAGDYV
                     LAAKAVDERGNSIVAETHVWVSGTAGQLAAAWRSDVAVVCDKPSYKVGETARALITAP
                     ADVSSVLLTVEDREIRYVRVVRLENGSATVELPIVEEYAPNTYVTATAVASRVMQAAS
                     REIAVETPETALIVEIQPNKASYRPGEVATYLVKTKNAQGDAVQAEISFALVDESIYA
                     VRPDTTPDIGRFFHGRRERSVSTENSFPVTYYGGADKEGGAESARKYFPDTAAWFPSV
                     VTDANGHAVLRVTMPDSLTTWRATVRAHARSTMVGQAQQKVVVTMPLSARLGLPRHYT
                     LGDKAMVAGVVHNDTNRARRVSVTLRADGADVAGRAKQYVTVPAHGQASVEWEIEPRT
                     VGKVTLSLAARSWFLRDAVEMTVPVLPFGDHAERVWAGETRPADESGATLEFELPEDA
                     LRGATRVQLEVSPGYAGVVQQALEFLVNYPYGCVEQTMSAFMPDVVAHRAFTRLGLAL
                     PRSQTELARMVDSGLARLYKYQHHDGGFGWWEFDQSDTWMTSYVLFGLGRAKEAGFDV
                     SASVIDRALDYLAQSPTRAKMSNAREYAFASFVLAEYGRLKGESLNEFVRFGRQLQAL
                     SSVADPLTTAHITLAASCAGQESMAAAGAQRLSAVATRDSGQAYWRTQDTAHWWRNET
                     AESTAWAMMALLRVDPTSQLVPEAARHLAAARTGSQWRSTRESAAAVLALVEYMIQAG
                     DDVFAERKAVVSVNGKETASLTLGGVGSTESESEAASMSASITLDPNLIMPGRNQISV
                     KAQGGAVLYSARATWYAPKDRVEAGGDCAVITREYFRIDRNASAPKGQKYSVTPINCE
                     VGVCEEVLVRVTVRALTDLEYMAFEDPLPSGFEISEEPVDAYSWYYWYDRSEARDSKM
                     VVFATRIPAGKTQAFEYVLVPERPGSYRVMPTEAWSMYYPGLRARGNSSSIVVRRSK"
     gene            complement(10417..11337)
                     /locus_tag="PLM74_02315"
     CDS             complement(10417..11337)
                     /locus_tag="PLM74_02315"
                     /inference="COORDINATES: protein motif:HMM:NF013988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMMECR1 domain-containing protein"
                     /protein_id="HON41951.1"
                     /translation="MRNGGPVVTIFLIVVIAAGIVLMARAGGQVSVFSPRFEDGQREA
                     EAEGEGRGQGQGKAEAELSEPARDAEPRAGALDAYGDVLAELRGADREQMERVILDLA
                     RRFTEASVRGDPLPVARDIISVSGVPDCVSGILYRRSAGVFVTIIRNNAVRACVGSIW
                     AHFPNLTQEISHFAAAAAARDLRRPPLEARELAGCSYAVSVVGRLERVSPGIAWDPRS
                     YGVFVRAGERSGVILPGEALTYAKQVSWALSEAGIDARMPYEMYRFQTVKFGGSLSLS
                     SGLGLLRGYFTWAQTTAWRGSSFHALRSFC"
     gene            complement(11434..12423)
                     /locus_tag="PLM74_02320"
     CDS             complement(11434..12423)
                     /locus_tag="PLM74_02320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HON41952.1"
                     /translation="MSGEDTRDDRFSAGESVSWRSMAANLALVAFKLVAGIVGRSSAM
                     VADAAHSASDMLATFGVIVSFRISKRPADEDHPYGHGKAEPVAAKLVGAILIVAGSGI
                     AIEAVRKIIARDFARPGLIALIAAVASIVIKETMYQIAATTARRIRSSALMAEALHHR
                     SDALSSVGTFAGILGGRLGMPILDPIAGFVVAVMIIWMGIRIIRASSDELMDAQTDPE
                     TVNAVRATAAGVEGVCDVHSVVTRKYGGDSYVDLRIGVDPSISVVMGHEIAMEVERTI
                     RLECPSVRGVLVHVDPESVHVDDPEGHPLPRQGRNGYDDTAKAMHGANGQCTE"
BASE COUNT         2607 a         4154 c         3629 g         2557 t
ORIGIN      
        1 aaccccgccc ccggaagcca cactacggtg ttaagcacgt tgaacatggt gtgtgcattg
       61 gctatctgcc gggcaatgcc gcccacaggc gaaacggcag tgacaagcca tgcaaaagga
      121 atgcgcagga tcatgaagat gatcgcgccg aaaacgttga acaacaggtg caccatggct
      181 gttcgtctcg ccgtatgatt cgtgccgata ctggccaaca gggccgtgat gcacgttccg
      241 atattgtcgc ccagcagtat cggtatggcg acttcgatgc ttatcaggtc gggcgacgca
      301 atagctaccg ccaaaagcat gcctatcgtc gccgcgctgc tctggattac cagggtcatc
      361 acaagccccg tgagcagagc cagaaccgga ctggacgcca gggtgcgcat ggcctttgcg
      421 aagaccgcac tgcctcttaa gggggccaca gctgcagaca tcgtcgtgag cccgaggaac
      481 agaatcccga atccgagaat gacctgaccc acgcttctcc acactttcct gcgagcgagc
      541 atgatcatcc cgaaccctat gcctattgca ggatatgcgt agtcggccag gttgaacgcg
      601 atcagctggg ccgtgaccgt agtgccgatg ttagcgccca ttatgacgcc gacagtctgc
      661 ctgagcgtca ttagcccggc attcacaaag ctgacgagca tgacggtagt ggcgctgctg
      721 ctctgcatga cggcggtgac aaccgccccg acgattaccc ccttcagggg cgtagatgta
      781 agcacttcca gaacatgctt catcttctcg cccgccgcgc gccgcagtcc atcgcctgtc
      841 aaggtcatgc catacaggaa taggcccagc ccgcctatca tcccgaagac catagccgta
      901 gtaccgccca tgctatcaga tcacccctgg actttgctca caaacagctg caccaatcta
      961 ggatcgaatt gtgttccggc gttacgcgtc aattcctcgc gggcggcatc tttcgtcatc
     1021 gccgctctgt acaccctctc gctggtcatg gcgtcgtatg catcgcacac agcaagaatg
     1081 cgcccccaga gcgatatatc tttgcccttt ctgccaaacg ggtatccagt tccatcatac
     1141 ctctcgtggt gatcgagtat cgcccggtaa tcgcggtcgt tgtatatctt gtccatgcct
     1201 cgaatgagtt cagcgcccca cccgggatgc ttcttcacct cggcgaattc gctctcggtc
     1261 agttctccgg gcttggtgag tatctcctgg ggtatgttta ctttgcccag atcgtgcaac
     1321 ctagcgatat acgctatcct ctcaacatca cccctcgaca ggttacaggc ctccgcaagg
     1381 tagctggcga gctgtgcgac gcgttcggaa tgtccggcgg tgtaatggtc cttggagtcg
     1441 atgatccgcg ccagcgcaca attgatctgc tcagcatttt ccttctgaac cagcgcgcgt
     1501 tgggtgtagt tcttgttcga gatgaagaac agctcggcta aggcgaaagc cacaaggcct
     1561 ccgtattcgt acacggctgc ggccatgagg cccatgacga ttgatggcac cactgaactg
     1621 accgagttaa gcactatcga tgggtcaacc cggaaccgga acacgggctt tgagagtagt
     1681 atctggaagt tgacgataat acccgagact attacccaca ctagacctga cacgcctgca
     1741 gccgccacaa accggaagtt acccaaagcg atgtcccgcc cgggccatat ggcgtggaag
     1801 gcgagccagg cgcatatcgc gcatagcgca gtgatcgccc tgttcaacac tagcttgagc
     1861 cctgtcagat atggagagtt gttctcacgg attatcctga actcctggat gctaataccg
     1921 cccagcatcg caaggatcgc cgaagtgcct gcatcgtact tgataatgca tgccatcatc
     1981 accggaagca tcatcacaac tacggttgtg cgcagaacga taactgacag cgatgatgcc
     2041 cagagcagcg aaacgaatac gacggccgac gcaagtggaa cctcccaacg aatcgtatat
     2101 gcgacggcca gagcggcagc tgagattatg gccagcgcat atgcatcgtg tagtacgtgt
     2161 cggtcacgtg gcacccattc tccctcccat ccccgtacgg gttgcaaggc ccgatccggc
     2221 gcccaaaaaa gacccgtcgc agcgggttaa ggtcgctcat gaaacgcgcg cgggttaccc
     2281 gcgattacct gaccaggcag gtgtcggcga aagccacgac cgacatcagc agggtcatta
     2341 aggccgctac gaacccggcc aaagcgaact tcttcatacg cttcacctcc ccatgttgtt
     2401 gcgctactcg ggaggctcag ctcatcttcc gctctcatct cgtccactgc gacgggaaca
     2461 atcacgctgt gtttccctaa gctatcctgc gctatcccgg ctcgtcgtca ctctgccatc
     2521 gccaggcgct gcagttgccg gttgagcgcc tgcaagaccg agcgcgctac ggatgtaggc
     2581 acgtcgtctt ttctgtaggc cgcacctatg tacctggttc gctggccagt ggatacgcaa
     2641 gtgacagtaa tctcagaaac cacccggtcg cccgatagct cacagctggc ctgaagacgg
     2701 tacccaccgc ccatcatgga ctccagcgca tccactgtgg caatcttgac tatgtcgcat
     2761 atgcagtcac catcggagca tcccgatgct ttgcctacgt gcgactgccc catgtaatca
     2821 agatggatct ctgcagtcac agtgcgggac gggccatact ccagagaaat cctgctgagc
     2881 acaggcctgg acccggagca tcgccccacg tcttcctcgg ccgcgctcac ctgcgctatg
     2941 cttactttgc ggtagtcgac tcgctcgccc acatccagca ggtagatggt ctcgatatcc
     3001 ttcttcatac gcttgatctc gtcctgatcg agatgcccgg agaccatcac gtggatctca
     3061 tcgccctctg ctccctgtac gattctggcc cgggccaccc cgggtagctg acacaggtgc
     3121 tcttcggctc gggagagctt ggcttcgcgc tcaccgccca tcaccacacc tccgatcatc
     3181 cacgctaagc gctgcgaagc attccgaaag acaattctac tcaccatcgc gaatcccttc
     3241 aaatgtgtcg gatgcgtaag gtataattga tgttacttac gccacaatcg gagagatgcc
     3301 gcttatgtca acagactcaa gttcaagaaa aagatcggta gccgccctgg ttttcacgac
     3361 taccatgtgg ggcctgtctt tcatcagcac gaaagtgctg cttaggcata tggcccccgt
     3421 tcaggttgcg tttcttcgcc atgcgatagc gtcagtggct acgtggatcg ccgtggcggt
     3481 cgcacggccc gatatcaggg tcgcgctgcg cgacctgcct tctatgctga ctgcagccat
     3541 cgtgggaata cctatctact actactttga gaatactggg ttgctgtatc tatcggccgc
     3601 cactgcttcc attattactg catccactcc ggccatgact gcagcaatgg agagcatctt
     3661 ctaccgcagg cgtcttccca tcgccggatg ggccggcata cttgtctcgg cagggggagt
     3721 agctctggtt gtgcagtcgg gcgccgcaca ggccgccgct gcgagcgttg caggcagaag
     3781 cgacttcctg ctcggagcgg cgatgcttct ggtgtcggca tttgcgtggt cgctgtacac
     3841 cattttcaac aggccgaacg ttgcgtccta cgggtccctg accacaaacg cgtatcagat
     3901 cactttcgcg acagcagtgt tgtatgtggc cacactacga aacggcattc catggcgcga
     3961 ggcattgtcc agtccccagg ttctgctcaa cttgggctat ctgggcatct tctgttcggc
     4021 tatcggatac ctgctgtacc tgtttgcatt gcggtctctg gggccaacgg tggtatcggc
     4081 attcattaac ctggtgcctg tgttcggcgt agcaggaagc gcgctcatcc tcggggagcg
     4141 ggtgacgttg gtccagctcg tggggggagc catagtcgta ctgggaatat tcgcagttga
     4201 acgaatgcag ggaggggcgt aatgaggaga tatacattgc tcgcatgcga catagacggc
     4261 acgctgctca gaagcgacgg tactgccagc tcgcgcactt tgaaggcact gcgccttgcc
     4321 gaaacatccg gagccatggt aatcatagcc acaggacgaa ggattgcgtc gaccacgccc
     4381 atcgcacgcc gactgggcat acactccccg tgtgtcgctc attgcggcgc ggtggtctat
     4441 gatccgtcga acgagtctat cctgatggcc agacagatac cgagacagat cgcgcttcgg
     4501 gcatgtcgcc ttgccggcag tgtaggcgcc gacgtggcgg tgcacgaatc cgtatactcc
     4561 ggcaggtcca tattcgtgac tacatcgaag gagcttgagg aagcggcaga gcattggcca
     4621 ttcatgaccc cctactacag gcttgtggac ggtttcgagc aggcgtgcct cgccgacccg
     4681 gtgcagctgt gcctcatggc tgacgccgat atcatcccag ggctgaaacg ccgattggca
     4741 tgcgatatgc cggagacgct ctcgatcgta gactacggga tcgtggaaaa cggcaagcac
     4801 atgctggaca tattcgccca cggagttgac aaagccgtgg gactcacctt cgttgccgac
     4861 ctgtacggca tcggcaagcc ggagactgtc ggttttgggg actcggtgaa cgatatcgag
     4921 ttgatatgcc atgcagggct gggggtcgcg atgggcaacg cgcccgacga catccgccgc
     4981 atagctgacc tagtggcgcc gtcaaatgac gacgatggag tggccgccgt aatagaggag
     5041 ctcgcagccg ccggcctgct cgggcgcggc gcggtgccaa gctgcataaa gcatgctagt
     5101 tcgcgactgc attgacaggt tcatataggt cttctgagac tagcaggcgc tggcgacccc
     5161 cttctggcgc ctcggtttcg gcccacaccc ttacccggta cccgggcgca ccggcgctgg
     5221 caacggcgtc ggcccctggc agcggcgggg acttggccct gccggtggga tcagggggcg
     5281 ccgcaggcct cggtgcaatc acctcaatga cctcggtgta cagcctcaca tccggcacag
     5341 cgctctggcc ctcaatccat acgctcacgc tatctgctcc aatccgagcc cgcacagtca
     5401 ccggagtcgc ccacacattc acccaacgaa gatcggccgc cccgaaggac acggtggcat
     5461 ccagcccggc agggagataa ggcacaggtc gcgagtgtgg ccgccgctca gtaatctcca
     5521 tacccgcaag gagtgcggca ttatagagag ttgaagacag ctggcatatg ccgcctgcaa
     5581 tgccgggctc gctccgaccg tcccgaatga ctctggccgg accgtagcca agttcgctgc
     5641 tgcgttcgcc cacgacttcg ttaaacgaga actcctgccc cggccctacc acagtcccat
     5701 tgagcttctc cgcagcgatc gatatgttat cgttgcggcc tcgctggtcc ggatcgagct
     5761 gagttgtgca tcgaccgatc acgtaaagat cgtttgcatc agccgaaccc gcgttctcct
     5821 tcgatagaaa gtgagcagca ggcgcatggg cgctctgccc ctggaagccg aggccaggcg
     5881 caagcctcag tccagccatg acggcgacca ctatcgccgc catgactatc ccaactatgc
     5941 agcgtctgca tcgcccagca agcacctgcc ctcacctcac atgacaagac agtgactatt
     6001 tcgaacgcct taccactatg gacgagctgt tcccgcgagc acgtagccca ggatagtaca
     6061 tggaccatgc ctcggtaggc atgactctgt acgagccagg tctttccggc accagtacgt
     6121 attcaaatgc ctgggtcttt ccggctggaa tgcgagtcgc gaataccacc atcttgctgt
     6181 cgcgagcctc gctcctgtcg taccagtagt accaagagta ggcgtccaca ggttcctcgg
     6241 atatctcaaa ccccgacggg agcgggtctt caaatgccat atactccagg tcggtcaagg
     6301 cgcgcacagt cacacgcaca agcacctcct cgcagacgcc gacctcgcag ttgatcgggg
     6361 tcacagagta cttctgcccc tttggagccg aagcgttccg atcgatccgg aagtactcac
     6421 gagttatcac agcgcagtcg ccgccggctt ctacgcggtc cttgggagcg taccacgttg
     6481 cccgcgccga gtagagtact gcgccaccct gcgcctttac tgagatctgg ttacggcccg
     6541 gcatgatgag gtttggatcg agtgtaatcg aagccgacat cgatgccgct tccgactccg
     6601 actccgtact gccgacaccc cccagggtca gactagccgt ttccttcccg ttcacgctga
     6661 ccacggcctt ccgctcagcg aaaacatcat cgcccgcctg gatcatgtac tcaaccaatg
     6721 cgagcacagc cgcagccgat tcgcgggtgg aacgccactg cgacccggtt cgcgctgcag
     6781 ctagatgacg ggcggcctcc ggcaccagct ggctggtagg atccacccta agcagcgcca
     6841 tcatcgccca cgccgtgctc tcggcagtct cattccgcca ccagtgcgcc gtatcctgag
     6901 tccgccagta tgcctgcccc gaatcgcgcg tagccacggc gctcagccgc tgggccccgg
     6961 cggcggccat gctttcctgg cccgcgcagg acgccgcaag ggtgatatgc gcggtagtca
     7021 gcgggtcggc aactgagctc aaggcctgca actgcctgcc gaaccggacg aattcgttga
     7081 gcgattcgcc cttcagtcgg ccgtactccg ccaagacgaa cgaagcgaat gcatattccc
     7141 tggcattgga cattttggcg cgcgtcggcg attgggcgag atagtcgaga gccctgtcga
     7201 tcacggaagc gcttacgtca aaccctgctt ccttggctct acccagtcca aaaaggacgt
     7261 acgaggtcat ccacgtatcg ctctggtcga attcccacca cccgaatccg ccgtcatgat
     7321 gctggtactt gtatagcctg gcaagccctg aatcgaccat ccgggcaagc tccgtctggc
     7381 tgcgcggaag agccaagcca agacgcgtga acgcccgatg ggcaacgacg tccggcatga
     7441 aagcactcat cgtctgctct acacatccgt aagggtagtt cacaaggaac tccagggcct
     7501 gctggacgac gccagcgtaa cccggcgata cctccagctg cacacgggta gcgccccgga
     7561 gcgcgtcttc aggcagctcg aattcgagcg tcgcgccgga ttcgtcggca gggcgcgttt
     7621 cgcccgccca cacacgctca gcatgatcgc caaacggcag cacaggcacg gtcatctcca
     7681 ccgcgtccct caggaaccag cttctcgcgg ctagtgacag tgtcactttc ccaaccgtgc
     7741 gaggctcgat ctcccactcc actgaagcct gaccgtgagc cgggactgtg acgtactgct
     7801 tcgcacgccc agccacatcg gcgccgtcgg cccggagcgt taccgacacc cttcgcgctc
     7861 ggttggtatc gttgtgaacc acgccggcca ccatcgcctt atcgcccagg gtgtagtgtc
     7921 tgggaagccc gagcctcgcc gagagaggca tggtgacgac caccttctgc tgcgcctgcc
     7981 cgaccatggt tgaacgcgcg tgcgctctaa ccgtagccct ccacgtggtt agcgagtccg
     8041 gcattgtaac acgcagaacc gcgtgaccgt tggcatcagt caccaccgac gggaaccacg
     8101 cagcggtgtc ggggaagtac ttccgggcac tctcagcccc gccttccttg tcggcgccgc
     8161 cgtagtaggt cacaggaaac gagttctcgg tgctgacaga cctctcgcga cgtccgtgga
     8221 agaacctgcc gatgtcgggc gtggtatcag gccgaacagc gtatatggac tcgtccacca
     8281 gggcaaagga tatctcggcc tgcaccgcgt ctccctgagc gttcttggtc ttcacaaggt
     8341 acgtggccac ctcaccgggc cgataggatg ccttgttcgg ctgtatctcc acgattaggg
     8401 cagtctcggg cgtctcaacc gcgatctccc gcgaggcagc ctgcatcaca cgggacgcga
     8461 ctgccgtggc ggtgacgtac gtgtttggcg cgtattcctc cacgataggc aattccaccg
     8521 tggccgatcc gttctccagt cgtaccacgc gcacgtaacg gatttcgcga tcctccacag
     8581 taagcagcac agaagagaca tcggcaggcg cagtgatcag ggccctggcg gtctccccta
     8641 ctttatagct gggcttgtcg catactaccg ctacatcgct gcgccacgca gccgcaagtt
     8701 gccctgccgt gcccgagacc cagacgtggg tctcggccac tatgctgttc ccccgctcgt
     8761 ccacagcctt cgccgcgagc acgtaatcgc ccgcctcggt tgccgtgagc tccaccatgg
     8821 catcgccgga agacgaagtc tccaccgcgc gcgtcgccac cggcttgtcg gtgtatcctg
     8881 tcttgtcgta agtcctcttc atcatgacga gctcgagttt ccggcccacg gacgagccgt
     8941 ccagtgccac agtcttgacg cggacctgga aacggtctcc cggacttacg aggtatgcgg
     9001 acgtcgatac acgcatatcg aatgagccca tagccaccag ggccgagttc ctgcccgaag
     9061 ctcgcttgtt ggtggcgtca atcacgtcca cggccataat gtacttgtag ttgcgtccgt
     9121 cggcacgagg cgcctcaaag gagatgaggg cccgaccctg agagtcggta ctcgcctcgc
     9181 ccgacatgac gaaatcttcg taatagtcgc cgtaaccgtc gtagtcgccg tagtcgtcgt
     9241 aaccgtattc gccatccgcg cccgacgccg ccgcagcgaa cgacccaatg cgctggcgat
     9301 agaccctgta ttcgacttgg gcattcgcca ccggcgcgcc gaagtagtaa ctggcttcgc
     9361 atatggcttg cagtgtgtcg ccggcgatgt acctgtcacg ggagaactta acatccaccg
     9421 accactcggg cttgcggtac tccgcgacac taaagctcga ccagtgctgc tcgccgccga
     9481 tggtggcgac tacagagtag acgccgagcg gcggttcgct acccagggtg aactcgccat
     9541 agaaagagcc ccagtcgttc gtagtgagtt cctgctttga cagaaggttg tcgcggggat
     9601 cgcggatctc gaccttcacg gcctgccccg cgggaatggt gtagcccgga tcgctcatgg
     9661 tcctggctat tcccttgaag tagacggtgt tgtcgggccg gtagatcggc cggtcggtat
     9721 agacgtaaac cttctgctgc cgcgcgtcga accagaagga tgaatagagc acagcaaact
     9781 catcgccccg cccacctgtc accatggccg atgcggcggg ctgcgacagc ggactggaga
     9841 aaagacccga atcatcggtc acgcctgtca ccgtgcgaga cgcggcggtt gccgaggcaa
     9901 gctggtcatc gccgccaatc cccatcagcg caacttgcac aggtacgcct gcggcaggtt
     9961 cgccggtcct gagatcgact gcgtagacta gcaggcgacc cgcagactgc ttggatatca
    10021 ggccgatacc gctgacagtg aaccacaccg tgtcctgctg tctgagctgc ttcgaccgag
    10081 tgacagccaa gtatgcgccc gggggcagcg gatccagctt cactgatgcc tccagattgc
    10141 ctccgggatt ggggatcctc acactcactg tgcgcacaag cgacctcccg ggcaggcgag
    10201 cggggttcat gctccacata gcgtccgggc cggattcgga gaaatggcgc gcaccgtcaa
    10261 accggtatat gtccaggtag agttggctga cgccccttcc cctcactcca agctcagcct
    10321 gttcacccgg caggtaagtc cgagccgtgg caaatagcca cgcataggct tgccgggcgg
    10381 ccattgcagg ctccgcggca agcaggcatg ccgccatcag cagaacgacc gcaatgcatg
    10441 gaacgatgat ccgcgccagg cggttgtttg tgcccacgtg aaataccccc tcaatagccc
    10501 gaggccgctg ctcaagctga gcgaacctcc gaacttgacc gtctggaacc tatacatctc
    10561 atacggcata cgagcgtcta tacctgcctc cgaaagggcc cagctcacct gtttcgcata
    10621 agtgagtgcc tcccccggaa gtatcacgcc cgacctttcc ccggcgcgta cgaatacccc
    10681 gtaggacctg ggatcccagg ctatccccgg cgacaccctc tcgaggcggc ccacgactga
    10741 cacagcgtag ctgcatccgg ccaattcccg cgcttccaat ggcggtcgcc tcaagtcgcg
    10801 cgctgccgcg gccgccgcaa agtgggatat ctcctgagtc aagttgggaa aatgcgccca
    10861 tatagatccg acgcatgcgc gcacggcatt gttcctgata atcgtaacga agaccccggc
    10921 agaacgccgg tagaggatgc cggacacaca gtcggggacg cccgacacgc tgatgatgtc
    10981 ccgagctacc ggcaatgggt cgccccggac agaagcctcg gtgaagcgcc tggcgaggtc
    11041 caggatgacg cgttccatct gttcgcggtc agcgccgcgc agttcggcca gaacatcgcc
    11101 gtaggcatcg agtgctccgg cgcggggttc agcgtcgcgg gcaggctcac tcagctcggc
    11161 ctccgccttg ccctgaccct gaccccgacc ctcgccctcg gcctcggcct cgcgttgacc
    11221 gtcctcgaat cgcggcgaga acacactcac ctgcccgcca gcccgggcca tgagcacaat
    11281 tcccgccgcg atcacgacga tcaagaaaat ggtcacgacc ggcccgccgt tacgcaatgt
    11341 ggatccagcc tccccggttc gccatctagt gtttttacat tctacagcac gaccggggaa
    11401 tcctacacag cagagaagac catatcaagg cgttcactcc gtgcactgcc catttgcgcc
    11461 atgcatcgcc ttggctgtgt cgtcatatcc atttcgccct tgacggggaa gcgggtggcc
    11521 ctccgggtcg tccacgtgaa cgctttcagg atccacgtga accagcacgc cgcgaacact
    11581 cgggcactcc agccttatcg ttctctcaac ttccatcgct atctcgtggc ccatcactac
    11641 ggagatcgaa ggatccaccc ctatgcgcag atccacgtag gaatcgcctc cgtacttgcg
    11701 cgtgacgacc gaatggacat cacacactcc ttcgacgccg gctgcagttg cccgtacagc
    11761 attgacggtt tcaggatcgg tctgtgcgtc cattagctca tcagatgatg cgcggattat
    11821 ccgaatcccc atccagatga tcatcacggc tactacgaat cctgctatcg gatccagtat
    11881 gggcatgcca agacgtcctc ccagtattcc cgcaaaggtg cccaccgagg aaagcgcatc
    11941 cgacctatga tgtagggctt cggccatgag agcgctgctc ctgatccgcc tggcagtggt
    12001 cgctgcaatc tggtacatgg tctccttaat tacgatagac gctactgcgg ctatcagtgc
    12061 gatcagcccg ggccgagcga agtcacgggc gatgatcttt cgcacagcct ctattgctat
    12121 gcccgaaccg gcaacgatca ggatcgctcc taccaatttg gctgccaccg gctcagcctt
    12181 tccgtgaccg tatggatgat cctcgtccgc cgggcgcttc gatattctga agcttacgat
    12241 aaccccgaaa gtggccagca tatcggaggc cgagtgggca gcatcggcca ccatggcaga
    12301 gctacgaccg acgatacccg cgaccagttt gaaggcgacc agtgcgagat tagccgccat
    12361 gctgcgccac gatactgact cgcccgcgct gaacctatca tcacgggtgt cctcgccaga
    12421 catgtatgca tcctccccca aatcctgttt gcgaaatgac gaaacggcac ccgcgggacc
    12481 tgtggatgga tcgcagtccc gcgagcgccg catgtatcgg acacccgctg cccctttggg
    12541 cccgatgccc cacctacaaa ggtgcaggca tctgctctga gcagatctca tcatgcacga
    12601 tgggagtgct cggtaccggg gttacgttca tcatgtatgc tatcccatcg cgtcgggagc
    12661 gtcaaggctc actcgagtca aggcggcacc ctctcccggg tcccggtccc gggggtgcgt
    12721 gtacggattg tggctttcgg agtcgcagat cccccgcatt caggcgtgtg gtatcgccaa
    12781 ggcacagcgg aggcagttgg agtcgcgcca aggaccaggg atagacccag gggtaggccc
    12841 agggacaggc ccgcgggtag gcccagcaat gggcccaggg gtgaacccgg ggatccgggg
    12901 gcgtgctgag gcagggaaag atgactgctg tggctcaggg atatacc
//
LOCUS       DAOTAC010000030        12780 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_4129_length_12780_cov_4.812809, whole genome shotgun sequence.
ACCESSION   DAOTAC010000030 DAOTAC010000000
VERSION     DAOTAC010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 12780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12780
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4129_length_12780_cov_4.812809"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2884
                     /locus_tag="PLM74_02325"
     CDS             <1..2884
                     /locus_tag="PLM74_02325"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HON41953.1"
                     /translation="TVSFREAYGITVDEIVESLINTMPPQVHLVLSTRERPSFGSLAR
                     WRVRGEALEITEKDLAFTSSEISGLFSGPYQHELTAAQVKVVLERTEGWIIALEMVWH
                     ALREGLSLPELFIRTPSSLESVFDYLAHEVLGKQEPYVTDFLLDVSALSELDPDVCGY
                     VSGRDDSESILSRLSTAGLFVYATDAGEGTFRFHNLLRTFLRRQARRDRMRWAECNRK
                     AAAYYGDRGQDTRVVDHLLAAGEYDGAARELIRLAPELVQSGRFEALAYWVGEMPGAV
                     LEFYPDLLLRRADVYRLSSNYRDALAWYSMADDIFADRSDAAGRSRALKGKAMVYLDT
                     VEPAMSDSLLSEALLLLPEEERLERATLLRMMAESKTNQGQMQEALKLVREAEQLISA
                     AMEDELDIRVHLRTGRLGAALASLQRRAGEEQQGRAAQGRSQQGLAADQACAEERGRP
                     ARSHRETQLLLSLVYSLIGEGEKARTCAEAAIQLGRDLKSPFVEAVGYMRLGHACHIS
                     PSASGAAAEACYRRALEMCDEIKVARGRAEPLMGLALVHAMSNDLARAEKEAAEALEI
                     CRSAGDEWLAGFVQIAMGAGMAYTGHDAGAASWLAEARETFARVGDEYCAAVCDLWLA
                     VLALRRHDSKTLRRILDGLFRSIRTHSYEFLFTKRTLFGPADLGVLAPLLVEAVRLGI
                     DREHVAWLHSATGLPSVEYHPGYTLHVRTLGRFVLYRGTEEVSNREWQREKARSLFQL
                     LVVNRKRYLHKDQILDALWPGLDSDAASSHFKVVFNAMLNVIEPGRPPRLNSFLINRE
                     GPLYGLNPLSGAWVDADEFESLVAKAGKLAPFDPQGACDLYRSAISLYRGDFLQECLY
                     EDWAIVERERLLAVYLRAAQSFAELLYGGHEIEECIDVCESILTRDMCWEEAYRLLML
                     CYLERGNRSMALRAYERCEAALQSEMGVNPSRDTAAVLERVRLSTT"
     gene            3110..3850
                     /gene="fabG"
                     /locus_tag="PLM74_02330"
     CDS             3110..3850
                     /gene="fabG"
                     /locus_tag="PLM74_02330"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014461644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HON41954.1"
                     /translation="MRLKDKVAIITGSGRGLGREAATLFAQEGARVVVCDINEQAAAD
                     TAKAISDAGGAAIWVRVDVTDPESVRVMVDRAMERFGRIDILVNNAGITADAMLVKMT
                     DDQWDRVINVNLKGVFNCTRAVAPTMIQQGSGKIISTASVVGIHGNFGQSNYIATKAG
                     IIGMTKGWAKELGRKGINVNAVAPGFTMTEMMSTVPEKVLTTIREKTPLGRLGEPKDI
                     AYAYLYLASEESNFVNGAVLQVDGGLVL"
     gene            3868..5163
                     /locus_tag="PLM74_02335"
     CDS             3868..5163
                     /locus_tag="PLM74_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HON41955.1"
                     /translation="MDLREVYRRKLISIEEAVGKIESGQSIVVAMCAAEPPGLLGAIS
                     SRKDELSDVTIMSCLLMRDYDFLRPEMRGHFLNEAWYYGPHSYRAHSGGTVTFLPNHL
                     HECGLKKKELDPPDVFWGTATPPDKNGYMSLSLSLTYEKPMIQAAKMVVLEINENLPR
                     TLGDTHIHISQVDYVVENTVPLVETQPIEPSDVEMEIGRNVASLIEDGSTLQLGIGGI
                     PNAVTACLMDKNDLGIHTEMITDGMVDLFNAGAVTGRRKTLWPEKMVGCFALGTNKLY
                     DFIDDNLGVELHGGHVTNDPWNIGRNYKMVSLNTTLQVDLTGQANSETLGTRQYSGGG
                     GALNTCMGAQMSPGGKSILALRATAKGGTVSTIVPCFKDGQSVTIPRADIDYVVTEYG
                     IAHLKSMNVRKRVDSLIAIAHPDFREWLRSEAKALQYIF"
     gene            5180..6181
                     /locus_tag="PLM74_02340"
     CDS             5180..6181
                     /locus_tag="PLM74_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HON41956.1"
                     /translation="MERYARVIGTGQYVPEIEVTNDEIAARVDKATPGLGAVVYKFEQ
                     NSGIRRRFYAPREWATSDLAVRAAQAALDDAGLKPEDIDLIILGTDSPDYITPATSVV
                     VQHKLGAANAGTYDVGCACASFPTAMAAGAGHLATNKWIKNVLVIGAYMMSKLADAAD
                     LMSFFYGDGAGAAVLTAGDRPGFITSSFLADGAYYKAWGIYSSGTAEPATCESVEAGR
                     TKVRLMDKYPAEVNHEGWPKLARLLAQRGGFTLDEVDQFIFTQVNQGSVGIVMETLGQ
                     PVEKAHCIMGNVGYTGSACIPMALDDAMKKGKIRPNDLVVLIGSGVGYNQAGVAFRM"
     gene            6397..7575
                     /locus_tag="PLM74_02345"
     CDS             6397..7575
                     /locus_tag="PLM74_02345"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HON41957.1"
                     /translation="MREAVIVSAVRTAIGTFSGTLANTSASELGATVIRAALERAKVE
                     PGAVDEVLMGCILQAGQGQNPARQAALKAGLPIEVPCTTINKVCGSGLKSVALAAQSI
                     IAGDAEIVVAGGTENMNLAPYMLDKARTGYRMGHGQLIDSMIHDGLWCIFGDTHMGIT
                     AENLAEQYSISRAEQDEFAAASQAKAAAAIESGRFADEIVPVLIPQRKGDPIKFDKDE
                     HVRPGTTVESLAKLKPAFKKDGTVTAGNASGINDGAAAVVVMSADRAKAMGLAPIAKV
                     RAYAAAGVDPSVMGIGPVPATRRALAKAGLTVEDIDLIEANEAFASQSLAVGRDLGWD
                     PAKVNVNGGAIALGHPIGASGARILVTLLHEMEKRDVKCGLATLCIGGGMGIAMIVER
                     "
     gene            7698..7946
                     /locus_tag="PLM74_02350"
     CDS             7698..7946
                     /locus_tag="PLM74_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41958.1"
                     /translation="MARETVIASFIMRFVKPGAAAATAGAGPGAGEATGSAAGGSGSH
                     AGVRIAVRHVQSGYERRFVNLDDAMDFIREEIRLLEQD"
     gene            7958..8827
                     /locus_tag="PLM74_02355"
     CDS             7958..8827
                     /locus_tag="PLM74_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HON41959.1"
                     /translation="MFGQQVVSGLSAGCLYALAALGLVLIYRTMDIVNFAHGEMAMVS
                     TFMAHTFLVRLGFSYTLAALGALIFAFIFGMAVERIFLRPIQGGPLISLMIMTLGLFM
                     VLNGAAGWVWGFDPVSFPRAVSGRPIWIGDLIITRDSILVLAVTIAMALALYLVLRFT
                     MAGIAIRATSQNSRAARLMGVPVPKVYALTWGISAVLGAVAGILIAPTTFLSPSMMAE
                     VQMKAFTAAVLGGFSSLPGAVVGGLLLGVLENLVAGYVSTELKSTFAFALIVVVLFIR
                     PSGLLGTPPKRKV"
     gene            8837..9871
                     /locus_tag="PLM74_02360"
     CDS             8837..9871
                     /locus_tag="PLM74_02360"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HON41960.1"
                     /translation="MERKTNNVLNGNEGLLRRIAGTPLGARLLLAAVLIVLPIITAAL
                     GRSYVVYLYSLVMVYVIVTLGLNILTGYTGQISIGHAGFMAVAAYTSAILTGRFGVPF
                     ILALIAASLLAAVLGLGLGVPALRLHGHYLAIATLGFGVAVGQLSASWDKLTGGYQGM
                     KAPVATILGFRFDTDLKYYYLVLALTWLLVEGAQNMLRSRPGRALMAVRDSEVAAQAM
                     GINLAKYKVTAFAVSAFYAGVAGVLHAHLTRYVSPYDFNMGVSMTLLSAIVVGGLGSV
                     PGSILGAIFMTILPHIFSRVKNLPLILAGLSLILVVLFLPYGLVSLPWKIKSAALGAR
                     ARRATKSGNA"
     gene            9858..10637
                     /locus_tag="PLM74_02365"
     CDS             9858..10637
                     /locus_tag="PLM74_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON41961.1"
                     /translation="MAMLELQGVTVRFGGLVAVNSLSFGVEEGTIHGLIGPNGAGKST
                     VFNVVSRFIRQDAGEIRFKGQALDCEPHEVVMRGIGRTFQNVELFRGQTVLDNVMIGL
                     HTAGKTDVLGGALQTPHVRQEEAALAERAYDTLGFLGIAEYAGRRAYGLPYGVQKLIE
                     LARALALRPSLILLDEPVAGMNPTERLELARVIRQTRSEFGMTVLLVEHDMSFVMNLC
                     DRITVMNFGSKIAEGAPSEVQQDPQVIQAYLGEEEQDAAAR"
     gene            10621..11340
                     /locus_tag="PLM74_02370"
     CDS             10621..11340
                     /locus_tag="PLM74_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON41962.1"
                     /translation="MLQLDSIDVSYGPVRALSGVSMEVPEGDIVALLGSNGAGKSTTL
                     RAISGIVRLTSGAIVFAGQRIDALSPERIVSMGVVQAPEGRQVFPELSVRENLLIGTY
                     SRRDRRRVPDDFNRVYMHFPILKERQTQAAGTLSGGEQQMLAIGRALMANPKLLLLDE
                     PSLGLAPLVTKQIFEIIRALNREGTTILLVEQNARLALAVSKYAYILETGRVALAGKS
                     EDLLATEEVKRSYLGAGHAAR"
     gene            11428..12615
                     /locus_tag="PLM74_02375"
     CDS             11428..12615
                     /locus_tag="PLM74_02375"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HON41963.1"
                     /translation="MKKILALLCVAMLAVMMAVGATAAAAPGITDNQVLIGAFQDQSG
                     AAAVVGINMRKGMEAYFNWVNAQGGVNGRKIKFIVEDDGFQAARAIAATKKLVEQDKV
                     FALVGTLGTPGVAATIDYIMEKQIPSIYQGSGISALAFPPKKYIFPVQPNFISEGRIV
                     ATYVVENLKMKRVALACEQTDIGTEGLRGVREQLATYKMDPVEVVNFGAADVDFSSQI
                     LKLMKAKPDAVIIYSTLKPAAGFLKQAKVMGLEAQFLTTYVNADPIQMPTLAGDACVG
                     LIAPGWVPVLGNDVDSVKYLEIYQATYPKEMPSAFAAAGFIAGEVFCEGLRRAGKNPT
                     RESLIAAMESMRDWDGIMAKGISYTPEFRSGKASMYFMRFAKPKFEEGSVEVISDWVK
                     LKI"
BASE COUNT         2589 a         3462 c         4222 g         2507 t
ORIGIN      
        1 gaccgtttcg ttccgggagg cttacgggat aacggtcgac gagatcgtgg agagcctgat
       61 aaacaccatg cctccacagg ttcacctggt cctatctact cgggagcggc cttccttcgg
      121 gtcgctggcc aggtggaggg tgaggggcga ggcgctcgag ataactgaga aggatctggc
      181 cttcacttcg tccgaaatat ccggcctgtt ttccgggccc taccagcacg agctcactgc
      241 agcgcaggta aaggtggttc tggagcggac tgaaggctgg attatagcgc tcgagatggt
      301 ctggcatgca ttgcgcgaag gattgtcgct cccggagtta ttcatccgca cgccatcttc
      361 gcttgagagt gttttcgact acctggccca tgaggtgctg ggcaagcagg agccgtacgt
      421 gacggacttc ctgctggacg tgtcggccct ctcggagctg gacccggatg tgtgcgggta
      481 cgtgtcggga agggacgata gtgaatcgat actgtcacgg ctttcaacgg caggcctgtt
      541 cgtgtacgcc actgacgcgg gagaagggac tttcagattc cacaatctcc tgcgcacgtt
      601 cctgaggcga caagccaggc gggaccgtat gcgctgggcg gagtgcaacc gcaaggctgc
      661 ggcctactac ggcgaccgag gccaggacac aagggtggtc gatcatctgc ttgctgcggg
      721 tgaatacgat ggagccgccc gcgagctcat caggctcgca ccggagcttg tacagagtgg
      781 tagattcgag gcgctggctt actgggtggg ggaaatgccg ggcgcggtgt tggaatttta
      841 ccctgacttg ttgctgcgca gggccgatgt gtacaggctg tcgagcaatt accgtgatgc
      901 tctggcgtgg tacagcatgg ccgatgatat cttcgccgat cgttcagatg ctgccggacg
      961 gagccgggcg ctaaagggca aggccatggt ctatctggac actgtggagc cggccatgag
     1021 cgattcgctg ctttccgagg cgctgctgct gcttccggag gaggagcgat tggagcgggc
     1081 cactttgctc aggatgatgg ctgagagcaa gactaaccag gggcagatgc aggaggcgct
     1141 gaagcttgtt cgtgaggcag agcagctgat cagcgcggct atggaggacg agctggatat
     1201 ccgtgtgcac ctgcgcaccg gcaggctggg ggctgcgctt gcatcgctgc agcgccgtgc
     1261 gggtgaggag caacaaggcc gtgcagctca gggccgttcg cagcaggggc tggcggctga
     1321 tcaggcctgc gccgaggaac ggggacggcc cgcccgttca caccgcgaga ctcagctcct
     1381 tctgtcgctg gtttactcac tgatcggcga gggtgagaaa gctcgcacgt gtgcagaggc
     1441 tgcaatacaa ctcgggcgcg atctgaaatc cccgtttgtg gaggctgtgg gctacatgcg
     1501 cctggggcat gcgtgccaca tctccccgtc cgccagtggt gctgccgccg aggcctgcta
     1561 taggcgggcg ctcgagatgt gcgatgagat caaagtggcg cgcggtcgtg ccgaaccgct
     1621 tatggggctg gcattggtac acgccatgtc caatgacctt gcccgtgccg aaaaggaagc
     1681 agcggaggcc ctggagatat gccggtcggc cggcgatgag tggctcgccg ggtttgttca
     1741 gattgccatg ggcgccggca tggcgtatac cgggcatgat gcgggcgcgg cttcctggtt
     1801 ggctgaagcc agggagactt ttgcgcgggt aggcgacgag tactgtgccg ccgtgtgtga
     1861 cctgtggttg gccgtgctcg cgctgcgccg ccacgattcg aagacgctcc gccgcattct
     1921 cgacgggctg ttcagatcta ttcgaacgca cagctacgag tttctcttca ccaagcgcac
     1981 gttgttcggc cccgccgatt tgggtgtcct ggctcctctg ctcgtagagg cggttcgcct
     2041 ggggatcgac cgcgagcatg tggcctggct tcattcggcc acggggcttc cgtctgtcga
     2101 atatcaccca ggctacactc tgcatgtgcg cacattgggc cgtttcgtgc tatacagagg
     2161 cactgaggaa gtgtcgaacc gcgagtggca gcgggagaag gcgcgcagcc tatttcagct
     2221 gctcgtcgtg aaccgcaagc ggtatctaca caaagaccag atcctcgacg cgctctggcc
     2281 ggggctggat tcggatgcag catccagtca tttcaaggtg gtcttcaacg ctatgctcaa
     2341 tgtgatcgag ccgggcagac cgccgcggct caactccttc ctgataaacc gcgagggacc
     2401 tctgtacggg ctcaaccctc tgtccggcgc ctgggttgac gcggacgagt tcgagagcct
     2461 tgtggccaag gccggcaagc tggcaccctt cgatccgcag ggagcgtgcg acttgtacag
     2521 aagcgcaatc agcctgtacc gcggcgactt tctgcaggag tgtctgtacg aagactgggc
     2581 catagtggag cgtgagcgcc tgctggccgt gtatctgcgt gcagcgcaga gctttgccga
     2641 attgctctac ggcgggcacg aaatcgagga atgcatagat gtgtgtgaat ccattctcac
     2701 acgcgacatg tgctgggagg aagcctatcg cctcttgatg ctgtgctacc ttgagcgcgg
     2761 caataggtct atggcgttgc gcgcctacga gcgatgtgaa gcagcactcc agtcggagat
     2821 gggggtgaac ccgagccgcg acaccgcagc tgtcctggaa cgcgtcaggc tatcaaccac
     2881 ctaaccacaa ccagcaaccc acaacccgcg atttgtaact cgtctgtaac ccgcgagtta
     2941 ccttcatgta acgccctcac agtattgtca gagtagcccg catagtcccc gatagcatca
     3001 tgagccatga gccacaaacc gtgagccata gaccgtgagt cataacgatg attgaccgca
     3061 ttatcggcca tcatgaacga cgacccatac taaggaggta ccaattctga tgcgactgaa
     3121 agataaggta gctataataa ccggttccgg tcgcgggctg ggcagggaag cagcgaccct
     3181 gttcgcgcag gaaggcgcga gggtagttgt gtgcgacatt aacgagcagg ctgcggccga
     3241 tactgcgaag gccatttcgg atgctggtgg agcagccata tgggtaaggg ttgacgttac
     3301 tgatcccgaa agtgttcgag tgatggtgga ccgtgcaatg gagaggtttg gccggataga
     3361 catcctggtc aataacgccg ggatcacggc agatgccatg ctggtgaaga tgactgacga
     3421 ccagtgggat cgcgtcatca acgttaacct caaaggcgtg ttcaactgca ctcgcgcggt
     3481 ggcgcccaca atgatccaac agggttcggg caagatcatc agcaccgcat ctgttgtcgg
     3541 aatacacgga aacttcgggc agtcgaacta catagccacc aaggccggca tcataggaat
     3601 gacaaaagga tgggccaagg aactagggcg taagggcatc aacgtaaacg cggtcgcccc
     3661 aggattcacc atgaccgaga tgatgtcaac ggtgccggag aaagtgctga ccacaataag
     3721 ggagaagacg ccattgggca ggctgggcga acccaaggac atcgcttacg cctatctgta
     3781 tctggcatct gaggagtcca acttcgtgaa cggcgcggtg ctgcaggtcg atggagggtt
     3841 ggtcctgtag ggcaaggagg gatagcattg gacctgaggg aagtgtatcg ccgcaagctg
     3901 atatcgatcg aggaggccgt tgggaagata gagtccggcc agtccatcgt ggtcgcgatg
     3961 tgcgcggccg aacccccagg gctcctcggc gccatttcgt ctcgcaaaga cgaactgagc
     4021 gacgtgacta tcatgtcttg cctactcatg agggactatg atttcctgag gcccgagatg
     4081 cgcgggcatt tcctcaacga ggcatggtac tacggaccgc attcgtacag ggcgcactca
     4141 ggcggcactg tcacgttctt acctaaccac ctgcacgagt gtggcctcaa gaagaaggag
     4201 ctcgaccctc ccgatgtgtt ctggggcacc gccacaccgc ccgacaagaa cggctacatg
     4261 tctttgtcac tcagcctaac ctacgaaaag ccgatgatcc aggcggccaa gatggtcgtg
     4321 ctggagatca acgagaacct cccacgcacg ctgggcgata cccatattca tatctcgcaa
     4381 gtggactatg tggtggaaaa caccgtgccg cttgtggaaa cgcaacctat cgagccttcg
     4441 gacgtggaga tggaaatcgg caggaatgtg gcttcgctga tcgaggatgg gagcacattg
     4501 cagcttggaa tcggaggcat tcccaacgcg gttacggcct gccttatgga caagaatgac
     4561 ctgggcattc acacggagat gatcaccgac ggtatggtgg acctgttcaa cgcgggagcg
     4621 gtgacgggca ggcggaagac gctttggccg gagaagatgg tcggctgttt cgcgcttggc
     4681 accaacaagc tatatgactt catcgacgac aacctgggag tggagctgca cggtgggcat
     4741 gtgaccaatg acccgtggaa catcggacgc aactacaaaa tggtaagcct caacaccact
     4801 ttgcaggtgg atctcacagg ccaggcgaac tccgaaaccc tcgggactag gcagtacagt
     4861 ggcggaggcg gcgctttgaa tacgtgcatg ggtgcgcaga tgtcgccagg gggaaagtcc
     4921 atactggcac tcagggcgac ggccaagggc ggcacggtat ctacgatagt gccgtgcttc
     4981 aaagacgggc agtcggtcac catccccaga gctgatatcg actacgtggt gactgaatat
     5041 ggcatagctc atctcaagtc gatgaatgtg cgcaaacgtg tggattccct gatcgccatc
     5101 gcacaccccg acttccgcga atggctcagg tcggaggcaa aggcacttca atacatcttc
     5161 taggatggag gcagtcctga tggagcgtta cgcacgagtc attggaacag gccagtatgt
     5221 acccgaaatc gaggtcacta atgacgaaat cgctgccaga gtcgacaagg cgaccccagg
     5281 gctcggcgca gtggtataca agttcgagca gaactccggc atccggcggc gcttctacgc
     5341 cccgagagag tgggccacgt ctgaccttgc ggtgcgggcc gcacaggctg cgctggatga
     5401 tgccgggctg aagcccgaag acatcgacct gatcatcctg ggcaccgaca gccccgacta
     5461 catcactcct gccacgtccg ttgtggtcca gcacaagctg ggcgccgcaa atgccggcac
     5521 ttacgatgtg ggctgcgcgt gcgcgtcctt ccccactgcc atggcggcag gcgccggcca
     5581 cttggccacc aacaagtgga tcaagaacgt gctggtcatc ggggcctaca tgatgagcaa
     5641 gcttgccgat gccgccgacc tgatgtcgtt cttctatggc gacggggcag gggccgccgt
     5701 gctcaccgcc ggcgataggc ccggattcat aacatcgtca ttcctcgcag acggcgctta
     5761 ctacaaagca tggggcatct actccagtgg aaccgccgag cccgctacct gcgagtcagt
     5821 cgaggcaggg cgaaccaagg tccgcctgat ggataagtac cctgccgagg tgaaccacga
     5881 aggctggccc aagcttgcgc gtctgctcgc gcagcgcgga ggattcaccc tagacgaggt
     5941 ggaccagttc atattcaccc aggtgaacca gggttcggtc ggaatagtaa tggaaacgct
     6001 cgggcagccc gtcgagaagg ctcactgcat tatgggcaat gtcggatata cgggctcggc
     6061 ctgcataccc atggctctcg acgatgcgat gaagaaaggc aagatcaggc cgaatgacct
     6121 ggtagtgctg atcggatcgg gagtgggcta taaccaggcg ggggtggctt tcagaatgta
     6181 gcgacaccga tgggtgttgc tcgtagagaa gaatgtgaag cagaacctga agatagcttc
     6241 cgatgcgtat gtcttggaaa accgcagggt agctctggag gggacgggtc catcagtgtt
     6301 gaatgattct catgtatgta gggtctatct tggagtatgg gcccggcata ttgccaaatc
     6361 tgtaagaaca tttcgaaatc gtggaggtag agaagcatga gggaagctgt catcgtctct
     6421 gctgtgagaa ccgctatcgg cactttcagc ggcactctcg cgaacacatc ggcgtctgag
     6481 cttggcgcca cagtcatccg agccgcgctg gagcgggcca aggtggagcc cggggctgtc
     6541 gacgaggtac ttatgggctg catcctccag gctggccagg gccagaaccc tgcgcgccag
     6601 gccgcgctca aggccgggtt gccgattgag gtaccctgca caaccattaa caaggtatgt
     6661 ggatccgggc tgaagtctgt ggcgctggct gcccagtcga ttatcgcggg ggacgccgag
     6721 attgtggtcg cgggcgggac cgaaaacatg aacctcgctc cgtacatgct cgacaaggcc
     6781 cggacgggat accgcatggg ccacggccag ctgatcgact cgatgatcca cgacggcctc
     6841 tggtgcatct tcggcgacac acacatgggc atcactgcgg agaacctggc cgagcagtac
     6901 agcatttccc gggcggagca ggacgagttt gccgcggcaa gccaggctaa ggcagcggcg
     6961 gccatcgagt ccggcaggtt tgccgacgag atcgtcccgg tgttgattcc ccagcgcaag
     7021 ggcgacccga tcaagtttga caaggatgag cacgtcaggc cgggcacgac ggtcgagtcc
     7081 ctcgcgaaac taaagcccgc cttcaaaaag gatgggacgg ttactgcggg caacgcgtcc
     7141 ggcatcaatg atggcgctgc agcggtagta gtcatgtcgg ctgatcgggc gaaagcaatg
     7201 ggtctggcac cgatcgcgaa ggttagagcg tatgcagccg ccggtgtgga tccgagcgtg
     7261 atggggatcg gccctgtgcc cgccacgcga agggcgctgg ctaaggcggg tctgacggtg
     7321 gaggacattg acctgatcga ggccaatgag gcgttcgcgt cacagtcgct ggccgtaggg
     7381 cgcgacctgg gctgggatcc cgcgaaagtc aacgtgaacg gcggggcgat tgctctgggc
     7441 catccgatcg gggccagcgg agcgaggatc ctcgtcacct tgctgcacga gatggagaag
     7501 agggacgtca agtgtggact ggccactcta tgcatcggcg gcggcatggg catagccatg
     7561 attgtcgaga gatagaccat tgagcctgcc ggggctggcc ggcgctctgc ccactcggag
     7621 cgtcggccaa tcccgcgggc cgagacttgg gaagcctggg acccttggga tatgagagag
     7681 cgcggaggat ttgatgtatg gctcgggaga ctgtgatcgc gtcgttcata atgaggtttg
     7741 tcaaacccgg ggccgcggct gcgactgccg gcgcagggcc gggtgctgga gaagctaccg
     7801 gctcggcggc gggcggatcc ggctcgcatg cgggcgtgcg gatagcggtg cggcatgttc
     7861 agagtggata tgagcggcgt ttcgtgaatt tggatgatgc gatggacttc atccgcgaag
     7921 agatccgcct gctcgagcag gactaggggg tggggatgtg ttcggccagc aggttgtgag
     7981 cgggctctct gcagggtgcc tgtatgccct ggcggcgctg ggacttgtgt tgatatacag
     8041 gaccatggac atcgtcaatt tcgcccacgg cgagatggcc atggtgtcca ccttcatggc
     8101 gcatacgttc ctggtcagac tgggattctc atatactctc gcagcgcttg gcgcactaat
     8161 attcgccttc atattcggga tggccgtaga gcgaatcttc ctcaggccga tccagggtgg
     8221 ccccctcatc agcctgatga tcatgacgct cggcctgttc atggtgttaa acggcgcagc
     8281 cggatgggta tggggctttg acccagtgag cttcccccgg gccgtttcag gccgccccat
     8341 ctggatcggg gatctgatca tcacgcgcga cagcattctg gtgcttgccg tgaccatagc
     8401 catggcgctc gcgctctacc tggtgctcag attcaccatg gcggggatcg cgatcagggc
     8461 cacgtctcag aactcgcgtg ccgccaggct catgggagtg ccggtgccga aggtgtatgc
     8521 gcttacctgg ggcatcagcg cagtgctggg ggctgttgca ggcatcctca tagctcctac
     8581 caccttcctt agtcccagca tgatggctga agtgcagatg aaagccttca ctgcggcggt
     8641 tctggggggc ttttcgagcc tgccgggggc tgtggtggga ggactgctgc ttggggtgct
     8701 ggagaacctc gtggcaggct atgtctccac tgagcttaag agcacgttcg ccttcgcgct
     8761 catcgtagtg gtgttgttca tcaggccaag tgggctattg gggacgcccc cgaaaaggaa
     8821 ggtgtgacgc ggcgcgatgg aacgcaaaac caacaacgtg cttaacggta acgaaggact
     8881 gctgcggcgc atcgccggta ctccgctggg cgcgaggctc ctactggcgg cggtgcttat
     8941 cgtgctcccc attatcacgg cggcgctggg ccggtcgtat gtggtgtacc tctatagcct
     9001 ggtgatggtg tatgtcatcg tgacgctggg cctgaacata ctgaccggct acaccggtca
     9061 gatctccata gggcatgccg ggttcatggc ggtagccgcc tatacgtcgg ccattctgac
     9121 aggaaggttc ggagtgccgt ttatactggc tttgatcgcc gcatcgctgc tggctgcagt
     9181 gctcgggttg ggcctgggag tgcctgcgct aaggctgcac ggccactacc tggccattgc
     9241 gacgctgggg ttcggggtgg cagtgggcca gctgtcggca tcgtgggaca agctgacagg
     9301 gggctaccag gggatgaagg ctccagtcgc aactatcctg gggtttcggt tcgacacgga
     9361 cctgaagtac tactacctgg tgctggcgtt aacgtggcta cttgtggaag gtgcccagaa
     9421 catgctgcgg agcaggcccg ggagggcgct catggcggtg cgcgacagcg aagtggcagc
     9481 gcaggccatg ggtataaacc tggccaagta caaggtgact gcgtttgcgg tgagcgcgtt
     9541 ttacgccggg gtggcaggag ttctgcacgc ccatctcacc aggtatgtca gcccgtacga
     9601 cttcaacatg ggtgtgtcga tgacactgct ttcagcgatc gtggtgggcg gattgggcag
     9661 cgtgcccggc tcgattctgg gggcgatatt catgaccatt ctgcctcaca tattcagccg
     9721 ggtgaagaac ttgcccttga tcctggccgg gctgtcgctg atcctggtcg tgctgttcct
     9781 gccgtacggt ctggtgagcc tgccgtggaa gatcaagagc gctgctttag gagcgcgggc
     9841 gaggagggcg accaagagtg gcaatgcttg agcttcaagg cgtgacggtc agattcggcg
     9901 gcctggtggc ggtcaactcg ctcagcttcg gtgtggagga aggcacgatc cacggtctta
     9961 tcgggcccaa cggcgccggc aagagcacgg tgttcaacgt cgtgtcgcgt ttcatacggc
    10021 aggacgcggg cgaaatccgc ttcaaaggcc aggctctcga ttgcgaaccg cacgaggttg
    10081 tgatgcgggg aatcgggcgt acgttccaga acgtagagct cttccgtggg cagacggtat
    10141 tggacaacgt gatgatcgga ctgcacaccg cgggcaagac cgacgttctg ggcggggcgc
    10201 tacagacacc gcacgtcagg caggaggaag cggcgctggc ggaacgcgcg tatgacaccc
    10261 tcgggtttct ggggatcgcc gagtacgccg gaaggagagc gtatggcctg ccgtacggtg
    10321 tgcagaagct gatagagctg gcgagggcac tggcgcttcg gcccagcctg attctgctcg
    10381 acgagccggt agccgggatg aaccctactg agcgcttgga acttgccagg gtgattaggc
    10441 agaccaggtc cgagttcggc atgactgtgc ttttggtgga gcacgatatg tcttttgtga
    10501 tgaacctgtg cgacaggatc actgtgatga actttggcag caagattgcg gagggcgctc
    10561 cgtctgaagt gcagcaagac ccgcaggtca tccaggccta cctgggagag gaggagcaag
    10621 atgctgcagc tagatagcat tgacgtctcc tacggcccag tgcgcgcgtt gagcggggtc
    10681 tctatggagg ttccagaagg tgacatcgtg gcactgctgg gctcgaacgg agcgggcaag
    10741 tcaaccacac tgcgcgcaat ctccgggatc gtgcgcctga caagtggggc gatagtcttc
    10801 gctggccagc gcatcgacgc gctgtcaccg gagcgcattg tctccatggg cgtggtgcag
    10861 gcgcctgagg ggcgccaagt ctttccggag ttgtcggtaa gggagaacct gctcatcggc
    10921 acgtattcgc gtcgcgacag aaggcgagtg ccggacgact tcaaccgggt ttacatgcat
    10981 ttcccgattc tcaaagaacg acagacgcag gctgcgggta cgctcagcgg cggagagcag
    11041 cagatgctgg ctattgggcg cgcgctcatg gccaatccca agctgttgtt gcttgacgag
    11101 ccgtcgctgg gcctggcgcc gctcgtcacg aagcagatat ttgagatcat cagggctctc
    11161 aacagagaag gaacgaccat tctactagta gagcagaatg cccgcctagc cctggcggta
    11221 tcgaagtatg catacatact cgaaaccggc cgtgtggcgc tggcggggaa gagcgaggac
    11281 ttgcttgcga ctgaggaggt gaagcgctct tatctagggg ccggccatgc ggccagatag
    11341 gcacgtgcat cggcggcaat cggcggaata tgcgggtgat ttgggcttgc tggtttcgcg
    11401 acgaatgata gagcaggagg ctagagtatg aagaagattt tggcattgct ttgtgtggcc
    11461 atgttggcgg tcatgatggc ggtcggcgcg actgccgccg cagctcctgg cataaccgac
    11521 aaccaggttt tgatcggggc atttcaggac cagtccggcg cggccgcggt agtgggtatc
    11581 aacatgcgca agggcatgga agcctacttc aactgggtta acgcgcaggg cggcgtgaat
    11641 ggacgcaaga tcaagttcat cgtcgaggac gacggtttcc aggcggcccg tgctatagca
    11701 gccaccaaga agctggttga gcaggacaag gtgttcgcgc tcgtcggcac gctgggcacg
    11761 cccggcgtag cggccacgat cgattacata atggagaagc agattccctc gatataccag
    11821 ggcagcggta tcagtgccct ggcgttcccg cccaagaagt acatcttccc agttcagccc
    11881 aacttcatca gcgagggtcg aatcgtggcc acgtacgtag tggagaacct caagatgaag
    11941 agggttgcgc ttgcgtgtga gcagaccgac atcggcaccg agggtctgcg cggcgtgcgg
    12001 gagcagctgg cgacgtacaa gatggacccg gtggaggtcg tcaacttcgg ggcggccgat
    12061 gttgatttca gctcgcagat tctcaagctc atgaaggcca agccggacgc tgtgataatc
    12121 tactccactt tgaagcctgc ggccggattc ctgaagcagg ctaaggtaat ggggctcgag
    12181 gctcagttcc tgacgacata cgtcaacgcc gatcccatcc agatgccaac gctcgccggc
    12241 gatgcctgcg tcggcctgat cgctccggga tgggtgcctg tgctcgggaa tgacgtggat
    12301 tcagtgaagt atctcgagat ctaccaggcc acctatccga aggaaatgcc cagcgcattt
    12361 gcggccgccg ggttcatcgc cggtgaggtg ttctgcgaag gcctgaggcg cgccggcaag
    12421 aaccccacgc gcgagagcct gatagctgcg atggagagca tgcgcgactg ggacggcatc
    12481 atggccaagg gtatctccta cactccggaa ttccgatcgg gcaaggcgtc catgtacttc
    12541 atgaggttcg cgaagcccaa gttcgaggaa ggctcggtag aagtgatatc cgactgggtc
    12601 aagctgaaga tatgaggttg agctcaaagc gttaggctat atgtcaaagc gaggccgggc
    12661 gaatcgcccg gccttcaaca ttgtaggatc ggtcccggca gatccgggct cataatccgg
    12721 cccgactccc cggcttgact gccggaagct tgccatcgcg gcatagaggg catcggtccg
//
LOCUS       DAOTAC010000031        12610 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_4201_length_12610_cov_4.651215, whole genome shotgun sequence.
ACCESSION   DAOTAC010000031 DAOTAC010000000
VERSION     DAOTAC010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 12610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12610
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4201_length_12610_cov_4.651215"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1195)
                     /locus_tag="PLM74_02380"
     CDS             complement(<1..1195)
                     /locus_tag="PLM74_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41964.1"
                     /translation="MPSIHRIQWFDAHVRTGRYPSARSLAERFEISHRQAQRDIEYMR
                     DSLGAPLEYCASRRGYRYIEDTFALPSLVVTAREGATLAALASQYSDIARLAFVSEGR
                     FGARYAEMANVLRRLGEAAVTDEPTEAASSDGHDRALHLPQPIPYTARLVPVGSLHGR
                     LPAGLEPYFGSADDDGSLVFTFPDASAFLSALLSAGIAFRVQSPAWLRRRLLAAADEL
                     AEANCDRPASDSESAQYDIPCRTAPATLNVSHTSKSLRGGAPGMRNSTGARLTPSWTS
                     YVGAAHGVLKAAGMIDLSMSQMMGMTGIGFHFIVHEECCPSSVTVYDWMSEHQQAMAR
                     IGVFAEPNMAEPGTPTYDAARRHTIHRIRESIDRGVGAILWGVDTGEFGVAYGYDDDD
                     QVLLVS"
     gene            complement(1618..1902)
                     /locus_tag="PLM74_02385"
     CDS             complement(1618..1902)
                     /locus_tag="PLM74_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015733014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HON41965.1"
                     /translation="MHLSNEHAKELIRIARKHGAMSVCIFGSYARGDADRESDLDVLV
                     EFEHGRSLFDLVRLERELRDALGVDVDVVTPNSLHPIIRERVMEERVPLL"
     gene            2139..3059
                     /locus_tag="PLM74_02390"
     CDS             2139..3059
                     /locus_tag="PLM74_02390"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HON41966.1"
                     /translation="MTTRRARYLWTILAVSVALSVCALGPARAMAASPAERDTCGIIA
                     FLTDWGTRDFYVGAAKGVAYSVFPEARLADITHEIEPYNIMEGAVTLLLAAREFPSGT
                     VFVAVVDPGVGTERRPIAVRTADGRFFIGPDNGIFTMVMQEFGVEEVRTITNRAFMRP
                     GPTSYSFHGRDIFTPTAANLAAGRKFAEVGPVADELILLDIKAARVENGKAYGEIVLV
                     DQYGNLQANIGRDLLAELGLTYGDTALVTVGGKTIPSKFVNTYGDVPEGDHLIFIAST
                     DWVEISINMDDAKAAFGAQIGSPVVIEKAR"
     gene            3237..4070
                     /locus_tag="PLM74_02395"
     CDS             3237..4070
                     /locus_tag="PLM74_02395"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HON41967.1"
                     /translation="MLKWCLEKHPGVAVFGSGKMIAYMTGFYIDEFLGVHKGALCPEW
                     AHASTNQESFETHRLMYQALGQKWVDDGCLTHAVYFLNYAREAQDAFVWNGFGSICVD
                     AVRAVEPIDVRAPEGIRITAIREEDIPVWLPLVDGHNRHVAKSPAFKPYLEPESPAEL
                     GNTLRQPGNLAWMAWHGGEAVGYMKVAPVEDGTAWIVNGEKKFAVNGAYVKPEYRSMG
                     IAKSLLSAVMEWALGEGFVRCSVEFEATNVEACRFWLKHFEPVCRSMVRRLDERILRT
                     L"
     gene            complement(4170..5105)
                     /locus_tag="PLM74_02400"
     CDS             complement(4170..5105)
                     /locus_tag="PLM74_02400"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HON41968.1"
                     /translation="MSRRQVALAVALLVVVGACGTCGAAPQGLWMQWILDPSHVSSNT
                     LIALHGLPGKTDGPMDWIDGPDGKWYRMDGSSNHFVIYSGATSGLALPSSAITVEAWA
                     MVSESANWAGLASFIQDNGSYERGWMLGCLDGRFMFGLASLGDRKVTDPYPSYLTYMK
                     ADGPYEIGKWYHVVGVYDGTTMRLYVDGRLAAESKDQHGVICQPDQGVLAIGAYVDND
                     ERHNLPGAIREVRIYSRALTADEVASSMPGSVVVSPDADAAQGIPVRLLMLDGSTIEG
                     FLVGAYGMDLLVAKGSATVQRIDSRQVGSVLMWPK"
     gene            complement(5155..5973)
                     /locus_tag="PLM74_02405"
     CDS             complement(5155..5973)
                     /locus_tag="PLM74_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41969.1"
                     /translation="MKRILALCTLAITLASFPLSIACGAPMTIVFNNGSMVTYDTDEI
                     KSITFSGTSATPVASPYGPPFAIVQPDIMEDFQNGLGNRWDPVAVVGGDLGRFARFES
                     GKLIVNVPQGNSWGKTGIQSKQPIFTVDRSFAAQPGLILIKTDPALTTGFVVTLASSW
                     HNDVWVLQTVWVHVVSATDEGEGSVVFINTQGPEKYGSDGLLPIKPVKAPEWVGLKVY
                     PGKVALQVSGMDSPAVIDMGWISEGTPVYLQVFSHPRAEGGPAAVAIDQIQVGR"
     gene            complement(6111..7481)
                     /locus_tag="PLM74_02410"
     CDS             complement(6111..7481)
                     /locus_tag="PLM74_02410"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HON41970.1"
                     /translation="MSRGANGSMHLDLCDDLVRRALAQGAADAEVFYRKVRKLEAMFE
                     KNDLQVPKGDTYEGVGIRVLLGGSNGARLGFAATNLLTPESLENALSAAIAIAHASPE
                     DANHSLPEPAPAKPVLGIYDQSAEELTLRDALDQGRLMLEAARGADPRVTLDSAMYTI
                     EVSEKAIVSSRGVRLSEKRSLFVCQAMAFARDGETVSSFDMEVGASSRMSGMDPAALG
                     RRLGEKVVATLGATTIPSFRGAVIIAPYAGMDLIVSPITFSSDAENVQTGRSKWKGML
                     GAQVLSPLLSVIDDPSIPGGASSTAFDREGVPARRLELVKDGVLNHYLYNCYTARKDG
                     VASNGRAAGTDQNTPSIAPTNFAIAPGERSLDEMMAACSKGLIVNRFSGNLDEVSGDF
                     SGVVKGGYYVEAGKIVCPVREAMIAGNIYDLLRQVTGVSRESVEIADIRLPYVQFDGC
                     SVTGKQ"
     gene            complement(7474..8883)
                     /locus_tag="PLM74_02415"
     CDS             complement(7474..8883)
                     /locus_tag="PLM74_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011430875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HON41971.1"
                     /translation="MAKSDREGDGMLDLLKAAVSGAAGYAEIRYHKREQTSIEAKNGR
                     LARVERRTLAGAGVRCLMDGCWGFVSTTDVTPGGLRKAISEAVEAAQIGAELRREKAV
                     LAPAKLAQGEFVYYDSAGEPSIGEKIQAVMAAEKRVRERSSLIVASSVRYLQYEDEKY
                     IVTSDGAAAHILDNKPSFHVIATAGRGDQLEMAIKSWGVTGSWKDLLDKTSLDKATND
                     AADLAVRKLGAGYVRGGQYTVILDPMLVGVLSHEAIGHTVEADFVQAGSVVQGKIGQK
                     VASDMITMVDDGTMAGAAGMVLADDEGVLGQRTVIIDRGILRDYLHSRESAQTFGAEP
                     HGNARAFTYGDEPLIRMTNTCILPGTDNLDQMIAGIDDGLLLREMGTGGGQADSTAEF
                     MFGVAEGYVINHGKLGELVKGITISGQAFEVLQSVDAVSSDFVCSLGAGHCGKWQPAK
                     VDSGGPYLRCRVTVGGRHE"
     gene            9496..9924
                     /locus_tag="PLM74_02420"
     CDS             9496..9924
                     /locus_tag="PLM74_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019550697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HON41972.1"
                     /translation="MAEPVCFDWSGYRRWMAQAANTLASARVDADHDFHNWCCFKAQQ
                     AGEYAVKAILHGLGVPAIGHSIVSLSERLREIGVETYEDFMTDARLLDRHYNPPRYPY
                     AYPSGNPHDYYDSAASGEALRACERVIELAHGVGRQVEEG"
     gene            10053..10730
                     /locus_tag="PLM74_02425"
     CDS             10053..10730
                     /locus_tag="PLM74_02425"
                     /inference="COORDINATES: protein motif:HMM:NF016365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HON41973.1"
                     /translation="MTGTLINAATVVAGTAVGLAVGKRMPERMSQAIIQGLGIFTVFI
                     GFKMAAETRNVLVALFAIVIGTAIGTALDIEGHLEIVAVGIERRFAKGGSGLANGFLA
                     ASLLYCVGPMSIIGSIEDGLSGDYRILMTKALMDGFCSIAFGATMGIGVALSALTILV
                     YQGGLTLAAAAVKGFLSDAVMTEMGAVGGLLIVGIGLNTLDVKKVHVGNMLPAIFVAG
                     ALARMLP"
     gene            complement(10799..11110)
                     /locus_tag="PLM74_02430"
     CDS             complement(10799..11110)
                     /locus_tag="PLM74_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41974.1"
                     /translation="MAQVLDGYLTRADDWHRRLLLSMSEPLEGVRPAVLTECTFVWLD
                     ELRRFRHLFRHAYGAELDRERVKDLVIQTLEMRSTLDEELCRLDAFVEALVKRHEEQD
                     T"
     gene            complement(11083..11268)
                     /locus_tag="PLM74_02435"
     CDS             complement(11083..11268)
                     /locus_tag="PLM74_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41975.1"
                     /translation="MSARSERACELRGLLVDMRTDMKAIDRLFRAADHAKVASLDRGS
                     DDMIVWAREWLRFSTAT"
     gene            complement(11252..11623)
                     /locus_tag="PLM74_02440"
     CDS             complement(11252..11623)
                     /locus_tag="PLM74_02440"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HON41976.1"
                     /translation="MDSHGFDQYLEHEDRIVAQREKDLAAHKERLARQAARLTEVLSA
                     LGARRIWVFGSFARGCVRSDSDLDMAVEGLPYSQDQLLGIAEQSLGGGLALDLVPWEA
                     AGALLQERILSEGRVLYEREE"
     gene            11798..12043
                     /locus_tag="PLM74_02445"
     CDS             11798..12043
                     /locus_tag="PLM74_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HON41977.1"
                     /translation="MIRMQVQLTEEQIEGLRAMASAEGVSVAELIRRGADMVLAGRGS
                     ISREERVRRALSIAGKFRSAETDISVNHDKYLAEDFL"
     gene            12040..12462
                     /locus_tag="PLM74_02450"
     CDS             12040..12462
                     /locus_tag="PLM74_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HON41978.1"
                     /translation="MMSVFVDTSALFALLSSNDVNHDAAWRVWEGLLRDETPLVCSNY
                     IAVEAAALVQRRLGMEALRAFRECICPMMDVEWVDRTAHDSAETALLTANRRSLSLVD
                     CTSFIIMRRLGITRAFTFNAHFAEQDFVCLPEMRLHDR"
BASE COUNT         2489 a         3723 c         3802 g         2596 t
ORIGIN      
        1 ccgagaccag caagacttga tcgtcgtcat catacccgta cgccaccccg aactccccgg
       61 tgtcgacccc ccaaagtatg gctccgactc cccggtcgat tgactctcgg attcggtgaa
      121 tcgtgtgccg acgcgcggcg tcgtatgtgg gcgtgcccgg ttcggccatg ttgggctcag
      181 cgaaaactcc gatcctggcc atggcttgct gatgctccga catccagtca tacaccgtca
      241 ccgacgatgg gcaacattcc tcgtgcacga tgaagtgaaa accgatacct gtcattccca
      301 tcatctggct catactgagg tcgatcatgc cggctgcttt cagcacgccg tgggctgccc
      361 caacataaga agtccatgaa ggagtgaggc gagcgcctgt cgagtttctc atccccggag
      421 cacctccgcg aagcgatttt gatgtgtgcg acacattgag tgtagcaggg gcggtgcgac
      481 atgggatgtc atactgtgct gattcgctgt cgctcgcggg gcggtcgcag tttgcctcag
      541 cgagctcatc ggctgccgca agcaagcgcc ggcggagcca ggctgggctc tgaactcgaa
      601 acgctatgcc cgcggagagc agggcagaca ggaacgcgct ggcgtcgggg aacgtgaaaa
      661 ctaggctacc gtcatcatct gcggatccga aatatggctc tagtcccgca ggcaggcgcc
      721 catgcagact tcctacgggc acaagccgag cggtgtacgg aatgggctgg ggaagatgga
      781 gcgctcggtc gtgcccgtcg ctcgaggcag cctcagtcgg ctcgtcagtg accgcagctt
      841 ctccaagacg gcgaaggaca ttggccatct cggcgtacct ggctccgaac cggccttccg
      901 agacgaacgc cagtcgggcg atgtcgctgt actgcgacgc cagagccgcg agggtagcac
      961 cctccctggc cgtcactacc aggctcggca gggcgaatgt gtcctcgatg taccggtagc
     1021 ctcggcgcga ggcgcaatac tcaagcggag ctcccagcga gtcgcgcatg tactcaatat
     1081 cgcgttgtgc ctgccggtgt gagatctcaa agcgttccgc caatgacctg gcgcttggat
     1141 atctgcctgt cctgacgtgg gcatcaaacc actgaatgcg gtgaatagac ggcatctgag
     1201 gctcccccaa acgagtagtt cggcgacgag ttcgacttcc ggcgccggca atcctgcata
     1261 agccgctgcc ggaacggcaa tggcagcttc ctcccttgat gatgaaggcc taagtggatg
     1321 acgggatcgc ccgatgggat gcttcgctcc cggcacggct gactctgaga cctgccggac
     1381 tgcctggcct tttgtcacgt gttcgaaaac gcgacaccca gattgtccca ctcccccctt
     1441 ggcccgccgc tacgcttttc ctggctacgc tttcctgctg ccgggctccg cttcgcttcg
     1501 tctatgcccg aaaccgcgag tacccatggg gatgggctca tttcgcagtg tagcgtccaa
     1561 tccgtgcaat gctatcagca atgtcctcta ggtaggggcg aacatcacgt gagcgaatca
     1621 tagtagcggc accctttcct ccatgacgcg ctcgcggatg atggggtgca ggctattcgg
     1681 ggtcactaca tcaacatcaa cgccaagcgc atccctaagt tctcgttcta ggcgaaccag
     1741 atcaaagagc gatctcccgt gctcaaactc aacaagaaca tcaaggtcgc tttcgcgatc
     1801 tgcgtcacca cgcgcgtacg aaccgaagat gcagacactc attgctccat gcttccgggc
     1861 aatgcggatg agttccttag catgctcatt cgagaggtgc atttcgacca acctccacaa
     1921 cgtgtcggtt cgttttctca gctcgtcact attctaccac atgctgggct cggcccgcat
     1981 cacttcccag ggggccttcc caggacccag gcccgtgagg acagactgtt acgtacgtac
     2041 agaaggaatg cccgagcgct atgttgaaca aagaagattg gcagaagcct ggcccgatct
     2101 gggagggcgg ggcgatcatt tacagggagg agatgcctgt gactacgaga cgagcaagat
     2161 acctctggac tatcctggcg gtgtctgtgg ctttgtcggt ctgcgcattg gggcctgctc
     2221 gggccatggc ggcgagccct gccgagcgcg atacttgcgg cattatcgca ttcctcacgg
     2281 attggggcac tcgcgatttc tacgtggggg ccgccaaggg cgtggcttat tcggtattcc
     2341 cggaggcacg cctcgcggat atcacccacg agatcgagcc gtacaacatc atggaaggtg
     2401 cggttaccct gctccttgcg gccagggagt tcccgtccgg cacagtattc gtggcggtcg
     2461 tggaccctgg ggtgggcact gagaggcgcc ccatagctgt ccggacggca gacggcaggt
     2521 tcttcatcgg cccggacaac gggatattca ccatggtcat gcaggagttc ggagtcgagg
     2581 aggttcgaac cataaccaac cgagcgttca tgcgccccgg gcccacatcg tactctttcc
     2641 atgggcgcga catcttcacg cccacggccg ccaacctggc ggcgggccgc aagtttgcgg
     2701 aggttggacc cgtggcggat gagctgatcc tgctcgacat caaggcggcg cgcgttgaga
     2761 acggaaaggc atacggagag atcgtgctgg ttgaccagta cgggaacctg caggccaaca
     2821 tcggtcgtga cctcctggct gagctggggc tgacatatgg agacacagcc ctggtgactg
     2881 tgggcggtaa gacgattccc agcaagttcg tgaatacata cggcgatgtt cctgaaggcg
     2941 accaccttat cttcatcgcc agtacggact gggtggagat ctctatcaac atggatgacg
     3001 ccaaggcggc ctttggggca cagatcgggt ctcctgtcgt gattgagaag gccaggtaaa
     3061 cgtgtgcgca atgtgggccg ggcagggtct gagggagtgg ctatatgcga gttgctgatt
     3121 tgaaacagtg tcatctggaa gaagcttgtg aactctttgc tgccacttat cgctatcaac
     3181 gtgaagcggt acccatactt gacggtagta acgcgagtac ggaaagagta ctgcccatgc
     3241 tgaaatggtg tctagaaaag caccccggtg tagcagtgtt tggcagcggc aagatgattg
     3301 cttacatgac ggggttttac atcgacgagt ttctgggggt ccacaaagga gccttgtgtc
     3361 cggaatgggc acatgcgtcg acgaaccagg aatccttcga aacccaccgc ctgatgtatc
     3421 aggcgttagg gcagaaatgg gtcgacgacg gatgcctcac tcatgctgtc tatttcctga
     3481 actatgcccg cgaggcacag gacgctttcg tctggaatgg ctttgggagc atatgtgtcg
     3541 atgccgttcg cgctgttgag cccatagatg tgagggcgcc ggagggaatc cggatcaccg
     3601 cgatacggga agaagacata ccggtctggc tacctctcgt agatggacac aatcggcatg
     3661 tggccaaatc gcctgcgttc aaaccatacc tcgagcccga aagcccggct gaacttggca
     3721 atacgttgag gcagccgggg aatcttgcat ggatggcatg gcacggcggc gaagccgtgg
     3781 ggtacatgaa ggtagcaccg gtcgaggatg gaaccgcctg gatagtcaat ggagagaaga
     3841 aattcgccgt gaacggcgcc tacgtgaagc ctgaatacag gagcatgggt attgccaagt
     3901 cgttgctctc agccgtcatg gaatgggcac tcggagaagg atttgtacgg tgctcagtgg
     3961 aattcgaggc aacgaatgta gaggcatgcc ggttctggct gaagcatttc gagcccgtat
     4021 gcaggtctat ggtgcggcgg ctggacgaga ggatattgcg cacactctga ggaggagctg
     4081 tacggagagg ctgtacagaa gacgaggggc gggatagacc cgccccttca ctggtctcca
     4141 agaagaacga gacagcttga agaagcccgt cacttgggcc acatcagaac agaacccacc
     4201 tgccgggagt cgatcctctg cactgtggcc gagcccttag ctacgagcag gtccataccg
     4261 taggcgccga cgaggaagcc ttcgatcgta gaaccgtcga gcatcagcag gcgtacggga
     4321 atgccctgtg ctgcgtcggc gtctggggag accactactg atcccggcat cgatgacgcc
     4381 acttcgtcgg cggtcagcgc tcggctatag atacgcacct cgcgtatggc tccgggcagg
     4441 ttatggcgct cgtcattgtc cacatatgcg cctatggcga gtacgccctg gtcaggctgg
     4501 caaatgacgc cgtgctggtc tttggactct gccgcgagcc ggccatccac atagagcctc
     4561 atcgttgttc cgtcgtacac ccctaccacg tggtaccact tgcctatctc gtaagggccg
     4621 tcagccttca tgtacgtcag gtatgaagga taggggtctg tcactttgcg atctccgagc
     4681 gaggccaggc cgaacatgaa ccgaccgtcg agacagccca gcatccagcc gcgttcgtag
     4741 cttccattgt cttgtatgaa gctcgcgagg ccggcccagt tcgccgactc tgagaccatt
     4801 gcccaggctt cgacggtgat ggcgctcgat ggcaaagcaa gaccgctagt cgctcctgag
     4861 tagatgacga aatggtttga agaaccgtcc atccggtacc acttgccgtc gggtccgtcg
     4921 atccagtcca tagggccatc ggtcttgccg ggcaggccgt gaagtgcgat cagtgtattg
     4981 ctcgacacgt gagacgggtc aagaatccat tgcatccaga gcccctgtgg agcggcccca
     5041 caggttccac aggcgcctac tactaccagg agtgcgactg ccagcgcgac ttgtctccga
     5101 gacatagggc tacacctcca attgcggact gaaccggcgg agcggacggg cagtctatct
     5161 gcccacctgg atctgatcga tagccacggc ggcaggcccg ccttccgcgc gagggtgcga
     5221 gaatacctgc agatacaccg gtgtcccttc gctgatccag cccatatcga ttactgcggg
     5281 gctatccatg cccgacacct ggagcgccac cttgccaggg tacaccttga gcccgaccca
     5341 ctcaggcgct ttcacaggtt tgatcggaag gaggccgtcg cttccgtact tctcggggcc
     5401 ctgggtgttg atgaacacga cggaaccctc gccctcgtca gtcgcgctga cgacatgcac
     5461 ccacacggtc tgaaggaccc acacatcgtt gtgccagcta gaggcgagtg tgaccacaaa
     5521 tcccgttgtg agcgctggat cggtcttgat cagtatcaag cctggctgcg ctgcaaacga
     5581 tcggtcaaca gtgaatatgg gctgcttgga ctggatgccg gtctttcccc acgagttgcc
     5641 ctggggcacg ttgacgatga gtttccctga ctcgaatctg gcgaaccttc ctaagtcgcc
     5701 tcctaccaca gcgacaggat cccaccgatt gcccagaccg ttctggaagt cctccattat
     5761 atccggttga acgatagcaa acggtggccc gtaaggtgat gctacgggtg tggcggatgt
     5821 gccggagaac gtgatcgact tgatctcgtc agtgtcgtat gtgaccatag agccgttgtt
     5881 gaagactatc gtcattgggg cgccgcaggc tatggagaga ggaaaggacg cgagcgttat
     5941 tgccagagtg cacagagcaa gaatccgctt catgaaatcg cctcctcaaa ttggcttacg
     6001 cctgacgaga ctgatgagcg atgattctgc aaatccgcgc cctttccttc gatccccatg
     6061 accgctgtgt cagaaaatct cgggctaccg ctgatcgggc agtattcgac ctactgcttt
     6121 ccagtgacgg agcacccgtc gaactggaca tacggaagcc tgatgtcggc tatctcgaca
     6181 ctctcgcgcg atacgccggt cacctgccgg agcaggtcat agatatttcc ggcgatcatc
     6241 gcttcccgga cggggcagac gatcttgccc gcctcgacgt agtagccgcc cttgaccacg
     6301 ccggagaaat ccccgctcac ctcgtccagg tttccagaga acctgttcac gatgagcccc
     6361 ttggagcatg cggccatcat ctcgtcgagt gaacgttcgc caggggcgat agcgaagttg
     6421 gtcggagcga tagacggcgt attctgatcg gtccctgcgg cgcgtccgtt cgaggcgacg
     6481 ccgtcctttc gggcggtgta gcagttgtag agatagtgat tgagcacgcc gtcttttacg
     6541 agctctagtc ggcgcgccgg taccccctcc ctgtcgaagg cggttgagct cgcgcctccg
     6601 ggaatcgacg ggtcatcgat tacagagagt agcggcgaca gaacctgtgc cccaagcatc
     6661 cctttccact tggaccggcc ggtctggaca ttctctgcat cagacgagaa cgtgataggg
     6721 gagacgatca agtccattcc tgcgtaggga gcgatgatca cagcgcccct aaaactgggt
     6781 atggtcgtcg cccctaaagt ggcgacgacc ttttcgccca agcgtcggcc tagagcggct
     6841 ggatccatgc cggacatccg gctcgacgcg ccgacttcca tgtcgaagga tgacacggtt
     6901 tcgccatcgc gggcgaacgc catggcctga catacgaata agctgcgctt ctcggagagg
     6961 cggactcccc gggagcttac gatggctttc tccgagacct ctatggtgta catcgctgaa
     7021 tccagagtca ctctcgggtc ggcgccgcgt gccgcttcca gcatgagacg gccttgatcc
     7081 agcgcgtctc ggagggtaag ctcctcagcg gactgatcat agatgccgag aactggctta
     7141 gcgggcgcgg gctcggggag ggagtggttt gcgtcctcgg gcgacgcatg ggctatggcg
     7201 atggcggcgg aaagggcatt ctctagcgac tcgggcgtga gaaggttggt agccgcgaaa
     7261 cccagcctag cgccgttcga tccgccgagc agcactcgta tccccactcc ctcgtacgtg
     7321 tcgcccttgg gaacctggag gtcgttcttc tcgaacatgg cctcaagctt cctgactttt
     7381 ctgtagaaca cttccgcatc ggctgcgcct tgggccagag cgcgccggac tagatcgtcg
     7441 cacagatcga gatgcatcga accattcgct cctctactca tgtcgtcctc caacagtgac
     7501 ccggcagcgc aggtaagggc ctccggagtc gaccttggcc ggctgccact taccacaatg
     7561 gcctgcgcca aggctgcaaa cgaagtctga cgagaccgcg tctacgctct gcagcacttc
     7621 gaaggcctgc cctgagatgg ttatgccctt gaccagctct ccgagcttgc cgtggttgat
     7681 aacgtagcct tcggccactc cgaacatgaa ctcggctgtg gagtcagctt gcccgccgcc
     7741 ggtgcccatt tctcggagaa gcaggccgtc gtcgatgccc gcgatcatct gatccagatt
     7801 gtctgtcccc ggcagtatgc atgtgtttgt catgcggatc aggggctcgt caccgtacgt
     7861 gaaggcacga gcattgccgt gcggttcggc cccgaaggtt tgggccgact cgcggctgtg
     7921 caggtagtcg cggagaatgc cgcggtctat gatgacggtg cgctggccca gaacaccttc
     7981 gtcgtctgcg agaaccatgc cggcggcccc tgccatagtg ccatcgtcta ccatggtgat
     8041 catatcggac gcgaccttct ggccgatctt gccctgcact accgacccgg cctggacgaa
     8101 gtcggcttcc actgtatgcc ctatggcctc gtgcgaaagc acccccacaa gcatggggtc
     8161 gaggatcacg gtgtactggc cgccccggac gtagcctgcg ccgagtttgc gcacggccag
     8221 atctgcggcg tcgttggtcg ctttgtccag actggtcttg tccaacaggt ccttccatga
     8281 gcctgtgacg ccccagctct tgatggccat ctcaagctgg tcgcccctgc cggcggtggc
     8341 tatgacgtgg aaactgggct tgttgtcgag aatgtgggcg gccgccccgt cgctggtgac
     8401 tatgtacttc tcatcctcat actgcaggta tctcactgag cttgccacaa tcagggacga
     8461 gcgctcgcga acacgcttct cggcggccat gactgcctgg atcttctccc cgatactggg
     8521 ttcgcccgct gagtcatagt agacgaactc tccctgggcg agcttcgccg gggcgaggac
     8581 agccttttcg cggcgcagct cggcgcctat ttgtgctgcc tccaccgcct cggaaatggc
     8641 ctttcgcagg ccgcccggcg tcacgtcggt ggtggagacg aatccccagc acccgtccat
     8701 gaggcacctg actccggcgc ctgcgagcgt gcgtcgctcc acccgggcca gtctgccgtt
     8761 tttggcctca atggacgtct gttcacgctt gtgataccgt atctccgcgt atccggcggc
     8821 tcccgaaact gccgccttaa gcagatcgag cattccatca ccttccctgt cggactttgc
     8881 catagcattc atgatccgat attcgcgttt ggtgtgcgtt caccttccag atgcgtcttc
     8941 cggcaaggtc ctggggcatg ctgcatccct gttcagatct gaacaggttt tccgcgctac
     9001 ctgttcagat cctgcccggt ttggcggggg ccatttgtca gaattcgcgc cggcagcggc
     9061 gcctcctttt tctggagcca gacccctcgt aaatccgcag gtagatgcgg ttcacataat
     9121 atggttgtct cttgacgtct cccgcgtctc cccgtaaaga cctccggacg ggcgcttgag
     9181 accataaaaa aacccaattc gtctgggtcg agtccccggc ccgtcaaaac cgagcacatt
     9241 ctgagcaggg ccggcggaaa accgggcaca attcttacgg gctcgctaac tccctgctag
     9301 acgtgggttt gcctaatagc ctgctcgcag ccgttttcgc caacagccgg cgactccgag
     9361 cgtaggcata cccccgcgcg aggtgttcgc cgcatgagca agactcatat cctttccagt
     9421 gatgcgggca atggaattcg gcagtaggta tttgctgcag agtggagaat gaacgtaggc
     9481 gggaggagga ggacgatggc ggagccggtc tgtttcgatt ggagtggata tcggcgttgg
     9541 atggctcaag cagccaacac acttgcgtcc gctcgggtcg atgccgacca tgacttccac
     9601 aactggtgtt gcttcaaggc gcagcaggcc ggggagtatg cggtgaaagc gatacttcac
     9661 ggcctgggcg ttccggcgat agggcattct attgtcagcc tgtcggagcg gctgagggag
     9721 attggagtcg agacatacga agacttcatg acagatgccc gactgctgga ccgacactac
     9781 aatccaccgc gttaccccta tgcatatccc tccgggaatc cgcacgatta ctacgatagc
     9841 gccgcttcgg gcgaggcgct ccgagcctgc gagcgcgtca tcgagctggc gcatggagta
     9901 ggccggcagg tcgaagaggg atgagattac tgaaggcggc gctcaccgaa ggggtcgtcc
     9961 tgctcgatcc gtatggagtg ttccaggtcc cttcgtccca ttgatcttct ggactgggac
    10021 gctgcaatca gaggaaggat acaggtactg ccatgacagg aacgctaata aacgcagcta
    10081 cagtggtcgc gggcaccgcg gtcggcctgg cggtggggaa gcgcatgccc gagaggatgt
    10141 cgcaggctat cattcagggg ctcggcatct tcacggtgtt cataggcttc aaaatggccg
    10201 ccgagactcg caacgtgctg gtagcactgt ttgccatagt gatcggcaca gctataggaa
    10261 cggcgctgga tatcgagggt cacctcgaaa tcgtcgctgt cggaatcgag cgacgattcg
    10321 cgaagggcgg ttcgggtctg gccaatggct tcctggcggc gagtctgctc tactgcgttg
    10381 ggcctatgag catcataggc tccatagagg acggactatc cggcgattat cggatcctga
    10441 tgaccaaggc cctgatggac ggattctgct ccatcgcatt tggcgccacc atgggcatag
    10501 gagtcgccct ctccgctctt acaatcctgg tgtaccaggg tggtctcaca cttgccgctg
    10561 cagctgtgaa aggttttctg agcgatgccg tcatgaccga gatgggcgca gtgggcgggc
    10621 tcctgatcgt agggatcggc ctgaatacac ttgacgtgaa gaaggttcac gtgggcaaca
    10681 tgctcccagc catattcgtc gccggggcgc tggcgcggat gctgccatag tcattgcagt
    10741 ggtctccgtg cggtcggacc ccacacgccc gaagcaatcg ggaagacgcg cttgtctatc
    10801 acgtatcctg ctcttcgtgg cgcttcacca aggcttcgac gaatgcatcc agtcggcata
    10861 gctcctcatc cagtgttgac cgcatctcca gggtctggat gaccagatcc ttcactcgct
    10921 cgcgatcaag ctctgcgccg taggcatgtc ggaagagatg gcgaaaacgg cgcagttcat
    10981 ctagccacac gaaagtgcat tcagtcagga ctgccggccg cacgccctcc aagggttcac
    11041 tcatgctgag aagaaggcgc cgatgccagt cgtcggcacg cgtcaagtag ccgtcgagaa
    11101 cctgagccac tctcgtgccc acacaatcat gtcgtcagaa ccacgatcga gcgaagctac
    11161 cttcgcgtga tccgcggcac ggaaaagccg atctatggct ttcatgtctg tgcgcatgtc
    11221 tacaagaaga ccgcgcagct cacacgcgcg ctcactcctc gcgctcataa agaaccctcc
    11281 cttccgacag gatgcgctct tgcagcaggg cgcctgcagc ctcccaaggt accaggtcaa
    11341 gagccagccc gccgcctagc gattgctcgg ctattcctag tagctgatcc tgtgagtatg
    11401 gcaggccctc cacagccatg tccaggtccg aatcagagcg tacacagcct ctcgcgaaag
    11461 agccgaagac ccagatgcgc cttgccccga gggcgctcag gacctcggtg agccgagcgg
    11521 cctgccgggc gagacgctcc ttgtgcgcgg cgagatcctt ctcccgctgc gcaacgatcc
    11581 gatcttcgtg ctcaagatac tggtcgaaac cgtgcgagtc cattgcgctc tgcctcctct
    11641 cgaccgctgg tcttcgagac gattatacca cacagccaga ttgtgtcagc gcggtgtgca
    11701 attcggtgtg caattcagat ggcgagctat gtccagagac atcagtatgt gatatcatga
    11761 taccatagga ggaaagactc actgaagggg gatgctcatg atccgcatgc aggtgcagct
    11821 cacagaggag cagatcgagg gcttgcgggc aatggccagc gcggaggggg tgtcggttgc
    11881 ggagctgatt aggcgcggcg ccgacatggt tctggccggt cggggcagta tcagtcgcga
    11941 ggagcgcgtt cggcgggctc tttcgattgc ggggaagttc aggtccgctg agactgacat
    12001 atcggtgaat cacgacaagt acctggcgga ggacttccta tgatgagcgt tttcgtcgat
    12061 acttcggcgc tcttcgccct actcagttcg aatgacgtca atcatgatgc ggcatggcgc
    12121 gtgtgggaag ggctactccg cgacgaaacg ccccttgtat gcagcaatta cattgcggtt
    12181 gaggccgcgg cccttgttca gaggcgactc gggatggagg ccctgcgggc gttccgggag
    12241 tgcatctgtc cgatgatgga cgtcgaatgg gttgaccgca ccgcgcacga ttcggcagag
    12301 accgctctcc taaccgcgaa tcgccggagc ctgagcctcg tcgattgcac cagtttcatc
    12361 attatgaggc gactggggat cacgagggcc ttcacgttta atgcccactt cgctgagcag
    12421 gattttgtct gcctgcccga gatgcgcctc cacgaccgat agctcaccag tgcctgatga
    12481 acctgccgag tgcgtgcttg aacagggcat tcgctcccgc gagggcgacc gccgctgcgg
    12541 cgagtacggc ggcaggcagg cccgggccgg catcgaccag gcgggccggc gcgttggcga
    12601 tcagcacgat
//
LOCUS       DAOTAC010000032        12582 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_4214_length_12582_cov_4.855991, whole genome shotgun sequence.
ACCESSION   DAOTAC010000032 DAOTAC010000000
VERSION     DAOTAC010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 12582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12582
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4214_length_12582_cov_4.855991"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="PLM74_02455"
     CDS             complement(<1..825)
                     /locus_tag="PLM74_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HON41979.1"
                     /translation="MGLYQVTIAITVGIFMMLAMGLNIITGYTGQVSLGHAAFFGIGA
                     YTSALLSVKAGVPFWLALPMAAVVTGAVGAILGAISTRLHDDSLAITTIGINFVVVSV
                     FQYSQFFGGALGVGGIEYPSVFGRALTKPEYLVVVIVCVSLGIWFDRRLVRSWLGLAL
                     RSVGQNPEAAESLGIPSARFKIAAFAIGTAYAGIAGSLYAHFMTFISARDFGFPTSIS
                     IISMAVFGGLGTLRGAILGSLALGLAPEIFRPLVNYRNLMYGALLLLMMRFQPSGLI"
     gene            complement(832..1692)
                     /locus_tag="PLM74_02460"
     CDS             complement(832..1692)
                     /locus_tag="PLM74_02460"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HON41980.1"
                     /translation="MLWQQLANGATMGMIYALITLGLTMVYGVLRILHISHAGIFALG
                     GYLAVLASRVSTNLAVVTAFAMLGCGVVGVLMQRWLYRPLLGESKVVSLIASIGMFTL
                     MEDLFRLVAGPYVLPLPVRAPFGLDRFETSHMTGNQLVVLIASVVLLIAAWFIIHRTR
                     LGLAWRATEQDFETASAFGVNIDRAVGLNFLLGSAVAGAAGALVGFYYNSVYPTMGSV
                     PAYKAMAIAVLGGLGNVWGTVVASLILGLVETFLVISPAGLILPRDSIAFVALILVLL
                     FRPHGLFGRR"
     gene            complement(1791..2954)
                     /locus_tag="PLM74_02465"
     CDS             complement(1791..2954)
                     /locus_tag="PLM74_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HON41981.1"
                     /translation="MRNPFRWLLIAFTGLVVCSLAVSVMGADPIKIGLHAPLTGFAAA
                     DGLSVRQSVELAVKQINDAGGINGRPIQLVIYDDRADAKEAVAVAAKLIEQDKVAAVV
                     GGSYSAPMRAVAPIYQQAKLPMVAGFAVHPDITRAGDFVFRVGMLGPIEGAAAGEVAG
                     KLLKAKTACQMTMDNDFGRALAEGFRARAAQLGIKIVWETLYPLGEMDFTPYLARLRQ
                     LNPDVVFTSGYYNEAAQFCKQAKEMGLKSQILGEEGFDSPKFIEIAGKAAEGVIIVTN
                     LNRDDARPAARAFIDGYRKLYKMEPDMVGASNYDAFLVIADALRRAKSTDPKAIRDAI
                     ASTRNFDGATGVISGFNALGECTKPIQAQVVKDGQFRYYGEVSDPAIITPPDK"
     gene            complement(3081..4154)
                     /locus_tag="PLM74_02470"
     CDS             complement(3081..4154)
                     /locus_tag="PLM74_02470"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HON41982.1"
                     /translation="MSSPLSGECSPVSVSCDGDRTASSSESLLPEGSGDPVGSRFCRN
                     AECTGHDPQRRPVLGYILALMAATIWGTLGIFAMLIYRYEIDPVVLVTARGVIASSVL
                     LITLFFTNRAALHIDTKDMPFFIAFGLVGVALNYLSYFEAIARTSATTAVILLYTSPI
                     FVTIAASLLFKEHFTPSKGLSLIVTLLGCFLVSGGYDPKRLVASPAGIAAGIAAAVTY
                     GSYTILSKRSLSKYRSSTTVLYALVFGSLFLALFSAARLGQLWGLPSQAWGLIVALAL
                     GPTLGAYALYVKALSHIEASHASIICMAEPAATAILAYLILGERMEVPQIVGAVTVVA
                     GVLILQLGPIASAHAQTRRSTPA"
     gene            4573..5310
                     /locus_tag="PLM74_02475"
     CDS             4573..5310
                     /locus_tag="PLM74_02475"
                     /inference="COORDINATES: protein motif:HMM:NF024934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HON41983.1"
                     /translation="MPDHLDRSHIGCVASVPIFRDLPRDTLLQLAAAVRHREFARGAL
                     IASYGDPIDNLIVVASGRLRLVHTSPSGREQIVREVGPGEFLGELGLVAAAQYDGDVI
                     AADDTRACMLSREAVRRAISFGPEASMRLIESLARRLARAEQVMGDLGARDVGQRLAA
                     ELVRAARGACECSGRRESDTVILPGPWSEVAARLGTTPESLSRRLRTFEDEGLISRPA
                     LEAESDLDGARRVVIVDLDRLREIAGL"
     gene            5783..6025
                     /locus_tag="PLM74_02480"
     CDS             5783..6025
                     /locus_tag="PLM74_02480"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HON41984.1"
                     /translation="MSDIRREMIILSGLNCPTCAAKLEKAVQSTPGVTEAKVVFGAGS
                     LNVEYDAEVVSYEKIADVVSRMGMAVTARVPGSTSR"
     gene            6031..7809
                     /locus_tag="PLM74_02485"
     CDS             6031..7809
                     /locus_tag="PLM74_02485"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HON41985.1"
                     /translation="MNLSTVNWLMIAVAIAAGAPIAKEAVTKLRMRQFSIALLVVLAS
                     IGAVLIGEAWEAAVVTVLYVLGGWLESRTLARTRSALRELVDAMPRTARVRRGDDIVE
                     VSAYEVVRGDIVVVRPGDLVPVDGEVVAGSASLDTSSVTGESMPMEAGVGARVTSGSV
                     SLGGYLEIRAEQVGADTTFSRLIYLVAEAGEQKPKVQRTLDRFAQWYTPSVISASVLV
                     YLISRDMHLALAFLVIACPGALVAAAPVAMIAGLGSSARRGVLIKGGERLERIAGIDT
                     VAFDKTGTLTLGRPRVVAVDAFEGSTPQQVLAAAASAELRSEHHLASAILARAEADGV
                     DPLPAHDWDLRPGQGVVAVTEDGRQAAVGNTALMKALGAAVSPEQLAAVESREAEGET
                     AALVSLAGRAIGLIGIADEPRPDAAMAITALRDAGIDRIVMLTGDNRRAALHIGAVLG
                     IHSDHIRAGLMPDEKVGAIRDLQATGARVAMVGDGINDAPALATADVSIAMGASATRA
                     AMEAADIALMSNNLMRIPHAMSHSRRILRVVRQNVALAVGVVVFLLAGVLAGRVHLAS
                     GMAVHEASILAVTLNGMRLLKQPRRG"
     gene            7929..8138
                     /locus_tag="PLM74_02490"
     CDS             7929..8138
                     /locus_tag="PLM74_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41986.1"
                     /translation="MAGDRTRQIRRVGALILVLLLIIAAVVLARWGNAGRTITSRTRI
                     EGASGDDADSRAPEGVDGDSCAPTG"
     gene            9054..10280
                     /locus_tag="PLM74_02495"
     CDS             9054..10280
                     /locus_tag="PLM74_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41987.1"
                     /translation="MGTINAGQGAGAVQSGAHDRLVLPGVARVGRRLGWGRNPERIPF
                     PSCLAACLRYIGDYYPWLPVREHSMDGRLDYSYIQTMGVTGMAFGLLWGPGWTSGNAN
                     LMSIADPDEIIDRAFRAAGRSCETMWRTDKPGDSDALRRTLVQSLELGRPVLAFGVIG
                     PPECCIITGYDYEEDAVIGWSFYQDDPRFAQGVEYEPNGCFRLQNWAQNTAGLIVVGD
                     RTDRGARPFDFAQSALATLNWALHVIHTPDAHGRKSGLAAYSAWAEQLICDSNFAMHD
                     EQQLWQRYAVHDSAVDTLAECRWTASRFLTLMADAVPELSAALLVAAEYYRQEYSLML
                     RIWSLVGGRGDPEAHTRFADPAVRRRIAGIVLSCRDSDARAAGMINQAVTAQITQCGR
                     QGKPANGTPRLPGARG"
     gene            10389..11240
                     /locus_tag="PLM74_02500"
     CDS             10389..11240
                     /locus_tag="PLM74_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HON41988.1"
                     /translation="MFRIMEKRLLAPAVHQLVVEAPLVARRARPGNFVIVRTNENGER
                     IPLTIADYDPEKGTVMLVVQEVGKSTMDIGMMEAGNSFTDVAGPLGQATPIKKVGTVV
                     GVAGGIGVAPLLPQIKLHKEIGNHVITIVGARTRDLLILKDETEQASHEVIFATDDGT
                     FGYHGFVTGALEELIKQGRQIDEVTAIGPVRMMQATCNLTRKYNIPTIVSLNAVMVDG
                     TGMCGCCRVTVGGETKFSCVDGPDFDGHKVDFDELASRQAFFRDEENLARELAEQKRG
                     GCRCHEK"
     gene            11219..>12582
                     /gene="gltA"
                     /locus_tag="PLM74_02505"
     CDS             11219..>12582
                     /gene="gltA"
                     /locus_tag="PLM74_02505"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HON41989.1"
                     /translation="MQVPREIVDRAEMPKQSPEERRSNFREVALGLDPELAVREAKRC
                     IQCKTRPCTKGCPVEVEIPEFIALMAQGKFQEADAKIKEKNSLPAICGRVCPQESQCE
                     SLCTLGKKFEPVAVGALERFAADWTREHNPESGCEKASSDGSARSLCAAADGVKRPSA
                     RIAVIGSGPAGLTCAAELALMGYEVDIYESLHETGGVLRYGIPEFRLPKAIVNQEVDY
                     VRSLGVRIFTNVLVGTTLDLNEMLDKGEYQAAFVGTGAGLPYFLEIPGENLNGVYSAN
                     EFLTRVNLMKAYRFPEYDTPVKIGKRVVVVGAGNVAMDAARTSLRLGAEDVKIVYRRS
                     RTEMPARLEEIENAEEEGVQMFLLTNPVRIISDGKGGVGAMECIKMELGEPDASGRRR
                     PVPVPGSEFTMDVDTVIIAVGQGPNPLLARATQGLALNRKGNIEVRDEYGATSIPGVW
                     AGGD"
BASE COUNT         2632 a         3579 c         3894 g         2477 t
ORIGIN      
        1 tatgagcccg ctgggctgga atctcatcat gagcagcagc agcgcgccgt acatgaggtt
       61 ccggtagttg acaagtgggc ggaagatctc gggtgctagg ccgagcgcca gcgaacctag
      121 aatggcgccg cgtagggtac ccaacccgcc gaaaaccgcc atagagataa tgctgatcga
      181 tgtagggaac ccgaagtcgc gtgcgctaat gaaggtcatg aagtgggcgt acagcgaacc
      241 cgcgatgcca gcgtaggctg tgcctatggc aaaagccgcg atcttaaacc gagcgctggg
      301 tattccaagt gactctgccg cttctgggtt ttgtcccact gaacggagag ccaagcccaa
      361 ccaggatctg actagccgcc tatcaaacca tatacccagc gacacgcaga cgatcacgac
      421 gaccaggtat tcaggtttcg taagggcgcg gccgaatacc gatgggtact caatccctcc
      481 gactcccagt gcgcctccga agaattggct atactgaaac accgagacga ctacaaagtt
      541 gatgccgatt gtcgtgattg cgagcgaatc gtcgtgcaat cgtgtgctta ttgcacccag
      601 gatagctccc acagctcctg tgactaccgc agccatcgga agagcgagcc agaaaggtac
      661 acctgctttg actgatagaa gggcagaggt gtacgcgcca attccgaaga atgcagcgtg
      721 ccccagcgag acttggcctg tgtagcctgt gatgatatta aggcccatgg ccagcatcat
      781 gaagattcca accgtgattg ctatcgtgac ttgatatagt cccatgtctg ctcacctccg
      841 tccgaacagg ccatggggcc tgaacagcag aactagtatg agggcgacga aggcgatgga
      901 atcacgtgga agtatgagcc ctgcgggcga tataacgagg aatgtctcca caagacccag
      961 gatgagagat gctactacag taccccagac gttgcccaga ccgcccagca ctgcgatggc
     1021 cattgccttg tacgcaggta cacttcccat ggtcgggtac acagagttgt aatagaaccc
     1081 taccagcgcg ccggcagccc ctgcaaccgc tgagccaaga aggaagttca gcccgactgc
     1141 gcgatcgatg ttgacgccga atgccgacgc agtctcgaag tcctgctcag tcgctctcca
     1201 tgccaggcca aggcgggttc ggtggatgat gaaccatgca gcgatcaaca gcaccaccga
     1261 tgcgattagg accactagct gattgccggt catgtgagat gtctcgaatc ggtcaagtcc
     1321 gaatggcgcc cgcaccggaa ggggaagcac gtaggggccc gccactagtc ggaagaggtc
     1381 ttccatgagc gtgaacatgc caatggacgc aatgagggac acgactttcg attcgcccaa
     1441 gagtggcctg tagagccatc tctgcatgag cacgcctacc actccacatc cgagcatggc
     1501 aaatgcggtc actacggcga gattcgtact gacgcgtgac gccaaaaccg ccaagtaccc
     1561 cccgagcgca aaaatgcccg catgcgatat atgaaggatt cgcaagacgc cgtagaccat
     1621 ggtcaggccc agcgtaatca gggcgtagat catccccatg gttgcaccgt tggccagctg
     1681 ctgccagagc aattgtgatc actccttact ggaagcgagt tgaacaagag ctggcggcgc
     1741 ctcacgtccg accgctcgcc ggctgtgaaa cgccgccaat agacggctct ctacttgtct
     1801 ggtggtgtga tgatggcagg gtcgctgacc tcgccatagt agcggaattg cccatccttg
     1861 acgacctggg cctgtatagg cttggtgcat tcacccaatg cgttgaaccc gctgattact
     1921 ccggttgcgc cgtcgaagtt tcgggtcgag gcgatcgcat cgcgtattgc tttcgggtcg
     1981 gtggacttcg cccgcctgag agcgtcggcg ataaccagga aggcgtcgta gttcgaggct
     2041 cctaccatgt ccggctccat tttgtagagc ttcctatatc cgtcgatgaa ggctctcgct
     2101 gccgggcgtg cgtcatctct gttcaggttg gtgacgatga ttacgccttc ggctgctttg
     2161 cccgcaatct cgatgaactt cggagaatcg aagccttctt cgccaaggat ctgggacttg
     2221 agacccatct ccttggcctg cttgcagaac tgggccgcct cattgtagta cccgctggtg
     2281 aacacgacat ccggattcag ctgacggagt ctagctaagt agggggtgaa gtccatctca
     2341 ccaaggggat agagagtctc ccacacaatc ttgatcccga gctgagctgc tctcgcgcga
     2401 aagccttcag caagggcgcg gccaaaatca ttatccatgg tcatctgaca ggcagtcttg
     2461 gctttcagaa gcttgcctgc aacttcgcct gcagcggcgc cctctattgg gcccagcatc
     2521 cccacgcgga atacgaaatc gcccgccctg gtgatatcgg gatggaccgc aaaccctgct
     2581 accataggga gcttagcctg ctggtaaatg ggagccacag ccctcatcgg ggcactgtag
     2641 gaaccgccga ccacggccgc gaccttgtcc tgttcaatca gcttggctgc caccgccaca
     2701 gcttcttttg catcggcccg gtcgtcgtag attaccagct ggatcggacg gccgttgatg
     2761 ccgcctgcgt cattgatctg ctttaccgca agctctacag actgcctgac actgagcccg
     2821 tcagcagcgg cgaacccggt gagaggagca tgcaacccga tcttgatggg atctgcaccc
     2881 ataacggaca cggccagaga acacacgacg agaccagtga atgcgatcaa gagccacctg
     2941 aatggattcc gcatcgttcg gcacctccaa atctgttttg tggacgatcc gctttctcca
     3001 tgatgcagac gtattgccta gatgaacggc aagacacctc ccgtagctag cccatcagac
     3061 gcacctcctc gcggtacaaa tcacgctggt gttgagcggc gggtctgagc gtgagcagag
     3121 gcgatgggcc cgagctgcag aatcagaacc cctgcgacta ctgtaaccgc tccaacgatc
     3181 tgtggcacct ccattctctc tccaagaatc aggtatgcga ggattgctgt ggcagcaggt
     3241 tcagccatgc agatgatgct ggcatgactg gcctcgatat gactgagcgc cttgacatag
     3301 agtgcatacg cgccgagcgt agggcccaaa gccagggcaa cgatcaaccc ccatgcctga
     3361 cttggcaaac cccagagctg gccaaggcga gccgccgaga acaaggcgag gaacagacta
     3421 ccgaaaacca gagcgtatag cacagtggtc gatgatctgt actttgacag cgaccgcttg
     3481 ctaaggatgg tgtaggatcc ataggtgacg gcagctgcga ttcccgctgc tattcccgcg
     3541 ggacttgcga cgaggcgctt tggatcatag ccgccggaca cgaggaagca accgagaagc
     3601 gtcacgatga gtgaaagccc ttttgacggg gtgaagtgct ctttgaagag aagagaggct
     3661 gcaatggtga cgaagatcgg agatgtgtac aataggatta cggctgttgt tgcggaggtt
     3721 cgggcaattg cctcgaagta cgatagatag ttgagggcca ctccgacaag cccgaatgcg
     3781 atgaagaagg gcatgtcctt tgtgtctatg tggagcgcag cgcgattcgt gaagaacaag
     3841 gtgatcagga gcaccgaaga cgcaataacg cctcgcgcgg tcacgagaac gacggggtct
     3901 atctcgtatc tatagatgag cattgcgaat atgcccaggg ttccccagat cgtggcagcc
     3961 atcaaggcca gaatataccc cagaaccggt cggcgttgcg gatcatgccc ggtgcattct
     4021 gcgtttcggc agaatcgaga cccgacagga tcgccggacc cctcaggcaa taggctctcc
     4081 gatgatgaag cagtgcggtc gccatcacaa gatacggaaa ccggcgaaca ttcgcccgat
     4141 agcggactgc tcaacgtgcc ccctcctcga tcaaccactc acggttgtac gggagataga
     4201 cgataactgt cgacgatcac cgctaaagga catgtttctt gggctgcata tgctactgct
     4261 tctagtgcga cctgttcgtc tgactctccg gtctgctacg cgcccagcgc ggacatggcc
     4321 tgggatcgca tgacagatga ctcaggtgga atacgcaatc tggtctgggt gatagatagc
     4381 tcaaacaagt attctgcgct gtgggcgcaa ttcctgcgag agcagccctg acggctttga
     4441 aggggtatca gtggatcgga tcagtcatat ggctgatcgg ccttgtttga cctcatggct
     4501 cgcttccgca atactatact tagtcactgt gagaatcgcc attgcatagt ctcgaaaggc
     4561 ggtgttcttg tcatgccgga tcatctcgac cgttcgcaca tcggctgtgt cgcaagtgtg
     4621 ccgattttcc gagacttgcc ccgcgatact ctgctgcagt tggctgcggc ggtgaggcac
     4681 agagagtttg ctcggggtgc gcttattgcg tcctatggtg accctatcga caacctcata
     4741 gtagtcgcat cggggcggct gaggttagtg cacacgtccc cctcggggag ggagcagatc
     4801 gtgcgcgagg tcggtcccgg tgagttcctg ggcgagctcg gactggtcgc ggctgcgcag
     4861 tatgatggag acgttatcgc cgccgacgac actcgggcct gtatgctctc aagggaggcg
     4921 gtccgccgag cgatttcgtt tggccccgag gcgtcgatgc gcctcattga gtcgctggcc
     4981 cggaggttgg cccgggcaga gcaggtaatg ggcgatctgg gcgcccgcga tgttggccag
     5041 cgtctggcgg cggaattggt ccgcgccgcc cggggcgcgt gcgagtgttc agggaggcgt
     5101 gagtccgaca ctgtgatact gcctggcccg tggtcggagg tggcggcccg gctggggact
     5161 acacccgaga gcctcagccg caggctgcgc acctttgaag acgaaggact catatcccgt
     5221 cccgctcttg aggccgaatc ggacctcgat ggggcccggc gagttgtcat cgttgatctg
     5281 gaccggttac gcgagatcgc cggcctgtag gctgctgggt cagcatgcat ggtgacagta
     5341 tgtgggatgt tgagggcccc atcggggttc ccaggggtcc tgttcatttc gtggcaggtt
     5401 ttccgggcaa cctgctcgga tcctgcctgg tttggcgggc actaattgtc tgaattcgtg
     5461 tccctctccc gagtctcccc tggaagtctc ccaaatgggg tttgagcccc ccaataatca
     5521 ccatatcggc tgggtttcgt ccgcggccca tcaaaaccga gcacattcta agcaggaccg
     5581 gcggaaaacc gggcacaatc cttgcaggct cgctaactac ctgctaaaca cggttttagc
     5641 gaaacgactg ctccaggccg ctttctcctg cggccgagat ccccaatggc aacactgctt
     5701 gacgcaaatc aatgcgtggc ccacagcgag tgtggcacac tgtaatcgac agcgcaaacg
     5761 ctgatcgaag gaggttgttc agatgtcaga tatccgtcgt gagatgatta tactctcggg
     5821 tctcaattgt cccacgtgcg ccgctaagct ggagaaggct gttcagtcca ctcccggggt
     5881 gaccgaggcg aaggtggtct ttggagcagg cagtctcaac gttgagtacg atgccgaggt
     5941 cgtgtcctac gagaagatcg ccgatgtcgt atcgagaatg ggcatggctg tgaccgcgcg
     6001 agtacctggg tcaacctcga ggtaagggcc atgaacctgt ctaccgtgaa ttggctgatg
     6061 attgctgtcg cgatagcggc gggcgctccc atcgcgaagg aggccgtgac caagctgcgg
     6121 atgcggcagt tcagcatcgc gctcctcgtg gtgctggcct ccatcggagc cgtactcata
     6181 ggcgaagcgt gggaagctgc ggttgtgact gtgctgtacg ttttgggagg ttggctcgaa
     6241 tcgcgcaccc tggcgcgcac ccgctctgcc ctgagagaat tggtggacgc gatgccgcgc
     6301 acagccaggg tgcggcgcgg cgacgatatc gtggaggtgt cagcctacga ggtagtcagg
     6361 ggcgatatcg tagtggtccg ccccggcgat ctggttccgg tggacggcga ggtcgtcgcg
     6421 gggagcgcct ccctcgatac gtcgtcggtg acgggagaat cgatgcccat ggaggccggg
     6481 gtgggagccc gtgtgaccag cggctcagtc tctctgggag gctacctgga aatccgcgca
     6541 gaacaggtag gcgccgacac caccttcagt cggctcatct acctggtggc cgaggcaggc
     6601 gagcagaaac ccaaagtcca gcgcacgctg gatagattcg ctcagtggta taccccgtcg
     6661 gtaatcagcg catctgtgtt agtctacctg atcagtcgcg atatgcatct cgctctcgcg
     6721 tttctcgtga tcgcctgccc cggcgccctg gtcgccgctg cgccagtcgc catgatcgcc
     6781 gggttgggct cgtcggcccg ccgaggcgtg ttgatcaagg gcggtgaacg cctggagcgc
     6841 attgccggca tcgacactgt ggcatttgac aagacaggca ccttaacctt gggcaggccc
     6901 agggtggtgg cggtagatgc cttcgaagga tcgacgccgc agcaggttct ggcagcggcc
     6961 gcctccgccg aactgcggtc agagcaccac ctggcatctg cgatactggc gcgggcggag
     7021 gccgacgggg tcgatcccct gcctgcccat gattgggacc tccgtccggg ccagggagtg
     7081 gtggccgtca ccgaagacgg gcggcaggca gccgtcggga ataccgctct catgaaggca
     7141 ctgggggctg ctgtctcgcc tgagcagctc gctgccgtgg aaagccgcga agccgaaggt
     7201 gaaacagcag ctctggtatc cctcgcgggc cgggcaatag gcctaatcgg aatagcggac
     7261 gaacccagac cagatgctgc gatggccata actgccctgc gtgatgcggg catcgaccgg
     7321 atcgtgatgc tcacaggcga caacaggcgc gccgctctgc atatcggcgc cgtcctcggc
     7381 atacattccg accacatacg tgcgggcctg atgcccgacg agaaggtagg cgctatccgc
     7441 gacctgcagg caaccggagc gcgagtggca atggtcggcg acggcatcaa cgatgcaccg
     7501 gcactggcaa cggcggatgt gagcatagcc atgggtgcct cggcaacccg ggctgccatg
     7561 gaggcggccg acatcgctct gatgagtaac aacctgatga ggatacccca tgccatgtcg
     7621 cattctcgga gaatactgcg ggtcgtgcgc cagaacgtag ccctggccgt gggagtggtc
     7681 gtgttcctgc tcgcaggcgt gctggccggt cgagtgcatc tcgcgtcagg catggctgtg
     7741 catgaagcca gcatacttgc ggtcacactc aacggaatgc gtctgctgaa acagcctaga
     7801 agaggctgat aagtgagacg cagactcgag aatggcaggc caagccatcg gcaggtaaag
     7861 ccgtatcgat agcgaaacac caagccaata atctgactcc gaatgagcct ttcgggaggc
     7921 gatggcggat ggctggggac cgaacaaggc agattaggcg cgtcggcgca ctgatcctgg
     7981 tgctgctgct gatcatagca gcggttgtcc tcgcaaggtg gggtaacgct ggcagaacga
     8041 tcacctctcg cacacggatc gagggcgcgt ccggagacga tgccgacagt cgcgcgcccg
     8101 agggggtcga cggagattcg tgcgcaccga ctggctgaag gccgtgacaa gcggcctgga
     8161 aggccgcacg gtggcgcctg acactagtgc acgacagatg cggaggggag agcgaagatt
     8221 ctagggcacg ctatgggcca caggaccacc ggatagggtc ccgcgccgtt cggtacctac
     8281 gtgttcgaca cagccggtta ttggcccgtc ctactcgttt tcggggaggt ttttcgctca
     8341 acctcctcgg attcttggtg gtttggtagg tacaaattgt ctgaattcgc gtcggcaacg
     8401 gcgctacctt tctccggagt cacacctgcg gcaaatctgc atgtagaggc cagttacata
     8461 atatggagat ccttgcgggt cccccgagtc ggccggaaag gcgctcggac ggctcgcgat
     8521 ctactaggaa accccagatt cgtgtggcct attccctctg agcgtcaaaa cctccagcat
     8581 tcaaaggagg tgcatcagga aacctcccac aattgttgta ggttctccga tcccctgcta
     8641 aacggctgtt tcgctaaagg gctgcgtaga gcctctttcg cttgccatgt gcgcagggaa
     8701 tgtactatgg aatgccgcga gattcccgat ccgtcccgtg cgccgtaggt tctccaacag
     8761 gccacacaga tccttgccca acccggctct ggctactcgg gtcttgtcgc gctcggaagc
     8821 cactgggcat taccgaagtg cccgatctgg ttaatctcgt atctcaactg cggaaatcgt
     8881 catcgtccgt gaccggcgtt cctccacaaa atggccgtat tgccgccaac ggaccatgtt
     8941 atactaaagg atagccgcca cgcctcactg cgcggcgacg gaccgggtgt tttcgcgacg
     9001 cccggtcctc agcatagttg tggctaatga aaggtgagga cggattgagt cacatgggaa
     9061 caatcaacgc tgggcagggg gccggcgcag ttcagtctgg cgcccatgat cggctggtgt
     9121 tgcctggtgt cgcccgggtc ggtcgccgtt tgggctgggg aaggaatccg gagaggattc
     9181 cgtttcctag ctgccttgcg gcctgcctca ggtacatcgg cgactattac ccatggctgc
     9241 ctgtgcggga gcacagcatg gacggtcgcc tcgactactc ctacatccaa accatgggcg
     9301 tgacaggcat ggccttcggc cttctgtggg gacccggctg gacctcgggc aacgccaacc
     9361 tcatgtcgat tgccgacccg gacgagatta tagatcgcgc cttccgcgct gccgggcgct
     9421 catgcgagac tatgtggagg acagacaagc ccggcgacag cgacgctctc cgccgcacgc
     9481 tggtccaatc cctcgaattg ggtagacctg tgctcgcttt cggcgtgatc ggcccgccgg
     9541 agtgttgcat aatcaccgga tacgactatg aagaggacgc cgtcatcggc tggagtttct
     9601 atcaggatga cccgcgattt gcacagggcg tggagtatga gcccaacggc tgtttcaggc
     9661 tgcagaactg ggcacagaac actgcgggcc taatcgttgt gggcgacaga acggaccgcg
     9721 gcgcccgacc attcgatttt gcccagtcgg ctctcgccac tctgaactgg gcgctccacg
     9781 taatccacac gcccgacgcg cacggtcgaa agagtggact ggctgcctat tcggcctggg
     9841 ccgaacagct catatgcgat tcgaacttcg ctatgcacga cgagcaacag ctctggcaaa
     9901 gatacgctgt gcacgacagc gcagtggaca cattggccga atgccgctgg accgcttcgc
     9961 gcttcctgac cttgatggca gatgcagtcc ccgaactatc ggcggccctg ctcgtggcgg
    10021 ctgagtacta tcgtcaggag tacagcctga tgctcaggat atggagccta gttggcggga
    10081 ggggagatcc cgaggctcac accagattcg ccgaccccgc ggtgcgccgc agaatcgccg
    10141 ggatcgtgtt gagctgtcgc gactccgacg ctagagctgc cgggatgatc aaccaggctg
    10201 tgactgcgca gatcacgcag tgcgggcgtc agggtaagcc ggcaaacggc accccgcggt
    10261 tgcctggtgc ccgcggttga cccgcagata tgtcaaggtt atgattgtat tcggggttta
    10321 gcactcgtta tcgttatgca gtatggctcg gtatacagta tggcttggta tgacgtgggg
    10381 gtgacttgat gttcaggatc atggagaagc gcctcctggc gccagctgtg catcagctgg
    10441 tggtggaagc acctctcgta gcgaggagag cgcgcccggg caattttgtg attgttagga
    10501 caaatgagaa tggtgagaga attccactca ccatcgccga ctatgaccct gagaagggga
    10561 ctgtcatgct ggtggtccag gaagttggca agagcacgat ggacatcggc atgatggagg
    10621 caggcaattc gttcacagac gtagcaggcc cgcttggaca ggccactccg atcaagaagg
    10681 tcggaacagt agtaggtgtg gccggcggaa ttggcgtggc tccgctcttg ccgcagataa
    10741 agctgcataa ggagatcggc aaccacgtca ttacgattgt gggcgcgcgc actcgcgatc
    10801 tgctgatcct taaggacgaa accgaacaag ccagtcatga agtgatattc gccaccgacg
    10861 acggcacctt cggataccac ggtttcgtca ccggcgccct agaagagctg atcaagcagg
    10921 ggcggcagat cgatgaggtc accgctatcg gtcctgtgcg catgatgcag gccacctgca
    10981 atctgactcg aaagtacaac atcccgacca ttgtcagcct caacgcggtc atggtggacg
    11041 gcacggggat gtgcggatgc tgcagggtta ccgtgggcgg cgagaccaag ttctcctgcg
    11101 ttgacggccc tgactttgac gggcacaagg tagacttcga cgaactggcg tcaaggcagg
    11161 ccttcttccg tgatgaggag aatctcgcac gcgaactggc cgaacagaag agaggtggat
    11221 gcaggtgcca cgagaaatag tcgacagagc ggaaatgccc aagcagtcgc ctgaggaaag
    11281 acggtccaac ttccgcgaag tggcactggg gctcgaccca gagttggcgg tgcgcgaagc
    11341 taagcggtgc atccagtgca agaccaggcc gtgtacgaaa ggatgcccgg tcgaggtgga
    11401 gattcccgag ttcattgcgc tcatggccca gggcaagttc caagaggccg acgccaagat
    11461 caaggagaag aacagcctcc cggcgatctg cggtcgcgtg tgcccccagg agagccagtg
    11521 cgaaagcctg tgcacccttg gcaagaagtt cgagcctgtg gctgtgggtg ccctcgagcg
    11581 gttcgctgcc gactggactc gggagcataa tcccgaatcc ggctgcgaga aggccagctc
    11641 cgatgggtct gcgcgttcgc tctgcgctgc ggctgatggg gtcaagcggc cgtctgcccg
    11701 catagctgta atcggctccg gccccgccgg gctcacgtgc gcagcagaac ttgcgctcat
    11761 gggctacgag gtcgacatat atgagtctct ccacgaaacc ggaggagttt tgcgttacgg
    11821 catacccgag ttcaggctgc caaaggcaat tgtgaaccag gaagttgact atgttcgcag
    11881 cctcggcgtc cgcatattca ccaacgttct ggttgggaca actctcgacc taaacgagat
    11941 gctcgacaag ggcgagtacc aggcggcgtt tgtcggcacg ggcgctgggc taccctactt
    12001 cctcgagatc cctggcgaga atctgaacgg cgtctactca gccaacgagt ttcttacgcg
    12061 tgtgaacctg atgaaggcct acagattccc tgagtacgac actcctgtta agatcggcaa
    12121 acgtgttgtc gtagttggcg caggcaatgt cgccatggac gcggcacgca cctcgctccg
    12181 cctgggcgcc gaagatgtca agatcgtcta caggcgttcg cgtacggaga tgccggcgcg
    12241 cttggaggag attgaaaacg ccgaagagga aggcgtgcag atgttcctgc tcaccaaccc
    12301 ggtccggatc atctccgacg gcaagggtgg agtcggcgcg atggagtgta tcaagatgga
    12361 actgggcgag cccgatgcca gcggtcgccg gcgtccggtg cctgtacccg gttctgagtt
    12421 caccatggac gtcgacactg tcataattgc ggtaggacag ggccccaacc cgctgctggc
    12481 cagggccact caaggtcttg cactcaaccg caaagggaac attgaagttc gtgacgaata
    12541 tggagcgacg agcattcctg gcgtgtgggc gggcggcgac at
//
LOCUS       DAOTAC010000033        12430 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_4295_length_12430_cov_4.450586, whole genome shotgun sequence.
ACCESSION   DAOTAC010000033 DAOTAC010000000
VERSION     DAOTAC010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 12430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12430
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4295_length_12430_cov_4.450586"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="PLM74_02510"
     CDS             complement(<1..296)
                     /locus_tag="PLM74_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HON41990.1"
                     /translation="MSSVGAATAVMADGLRKVYRVAVRKPGLWGSIRQLVACETRDVI
                     AVDGVSFDISQGEFVGYIGPNGAGKSTTVKMLTGILHPTSGLVEVNGLSPARARR"
     gene            complement(401..1264)
                     /locus_tag="PLM74_02515"
     CDS             complement(401..1264)
                     /locus_tag="PLM74_02515"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HON41991.1"
                     /translation="MERRCARPSAGAPGIVATAATRLRRAARTYEILVSASLRGKMQY
                     RLDFLGSTLIQTLMGADNILFIGAVLWRFGPVMGWSIFDIAMLYAVSRMGNGVYMLFC
                     NELDRFELYMVNGDFDTLLIRPWPTLFTLLARGVGIDRLAFLAQGIAAAALAAPRLVS
                     TGVIGGWDALWLLASVAWSALLYFAVGLATAAAGFWIVRIEELQVFTMNAPSTATAYP
                     LDVYPSWLKWVFVTLLPLGLGCYVPLRFILDKGGTALNLLWPALASPVAVWAALKLWK
                     TGEAHYTSTGT"
     gene            complement(1264..2061)
                     /locus_tag="PLM74_02520"
     CDS             complement(1264..2061)
                     /locus_tag="PLM74_02520"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HON41992.1"
                     /translation="MHAAVRQWRVLAGKGFAQNLQYLASHIVNTIASVVFGLIYIYVW
                     RAVTPESGFGAYSSSMIAQYICINQTTAWFTQFGMKIRQRMVQSVRSGRVAVELARPV
                     DFFWYHMSFEYGGQVYSFMFRGLAVGLVLSLLAQLRLPESASTWLGALASLAIGGYIG
                     ISLNYLVGISSFWTNEIRTLSWVLFSLEALLGGLSMPLEVLPRPLELLAKASPFAHLA
                     YYPARIYLELSGPAPILAAGLAWAAVLTIVARRVTSRARRRLEVQGR"
     gene            complement(2762..3199)
                     /locus_tag="PLM74_02525"
     CDS             complement(2762..3199)
                     /locus_tag="PLM74_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HON41993.1"
                     /translation="MSVYADTSALYAVIDADDANHCAARSTWVELLEGDTPLVCSNYV
                     LLETCALIQHRLGVDAVRGFRKSVYPVLQVEWVDKRMHETAVTAVVTANRRGLSVVDC
                     VSFEVMRVLGIDTAFSFDAHFEEQDFICLPHVGLHDRSAHLGE"
     gene            complement(3196..3435)
                     /locus_tag="PLM74_02530"
     CDS             complement(3196..3435)
                     /locus_tag="PLM74_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HON41994.1"
                     /translation="MQVQLTEEQVEALRAMAASEGVSISELVRRGADMVIGQARTMNR
                     REQVARAIAIAGQFRSGKSDVSVNHDAYLEEEYDA"
     gene            complement(3567..4004)
                     /locus_tag="PLM74_02535"
     CDS             complement(3567..4004)
                     /locus_tag="PLM74_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41995.1"
                     /translation="MKVFTPKDPTGPTIVAVLLIAIVMVVMFWGEIAAGGVGAAVVVG
                     TWVVLAALAWRAARSMADRKIVVDGNMVELVKGTSSKRVDLSGVESLEQRRVKSGRTT
                     YTAFLARLGSGELVELFADGQFSDQDGLKSAIADAAGKPWTTG"
     gene            4301..4792
                     /locus_tag="PLM74_02540"
     CDS             4301..4792
                     /locus_tag="PLM74_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070191 - methionine-R-sulfoxide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HON41996.1"
                     /translation="MLEGALDYPEDKAQLYVLLQTELRALVEGVPQPIPNLANASALL
                     WQGLRNINWAGFYLMDDGQLVLGPFQGKPACIYIPVGKGVCGTAVEEGRALRVDNVHD
                     FPGHIACDAASNSEIVIPIRDGERVVGVLDIDSPLLARFDSEDQDGLEGIVRILERAC
                     VWP"
     gene            4825..6024
                     /locus_tag="PLM74_02545"
     CDS             4825..6024
                     /locus_tag="PLM74_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019497521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HON41997.1"
                     /translation="MSIRRGIIAFVLLLCITVSSATAMGQIVTPEQMLKQIFTAEVMS
                     EDWFSAAFLAQVPVGQLSEIVRQYRAALGALEDVQGKAPAFRLTFSHGYVAAQIALDG
                     AGRVAGFFLGPPEPRVSGLDNALDGFRKLPGRVSVLVVSQSGVLAQLNPEQPLAVGSA
                     FKLAILAALKDRVAAGALAWDDVVTLEASHISLPSGILQSWPVGSPLTVHTLASLMIS
                     VSDNTATDALIALLGRELIEEYTALNHPLLATREAFALKNPANADHLKAYREGDMAQR
                     REVLKQLQSLPLPGVADFTSPQALDVEWFFSASELANLMAAVQDLSVFSINPGVASPA
                     DWQHVAFKGGSEPGVINLTTWLVGHSGMNYCVVATWNNDEVLDEVQFIGLYSGIIRQL
                     AQIDAKP"
     gene            6460..7161
                     /locus_tag="PLM74_02550"
     CDS             6460..7161
                     /locus_tag="PLM74_02550"
                     /inference="COORDINATES: protein motif:HMM:NF024246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HON41998.1"
                     /translation="MDRQTIAIDESKCDGCGACARACAEGVIEIIDGVARLVNEDHCD
                     GLGACIGDCPNGAISLVTVVCEPATAEAASGGPADGSSGRQTAGCPLWSISLDSVSTD
                     AGGQETAKSPSRAVPNWPIQVNLVSPTARWLAGSDLLVCADCVAYTCPDFHSRHLPGK
                     TLLVGCPKFDDRERFVSRLAEILLVAQVRSIEALHMDVACCSGLGRLVEEAQERAGTQ
                     VPVSTTVVGVDGRVL"
     gene            7345..9078
                     /gene="hcp"
                     /locus_tag="PLM74_02555"
                     /gene_synonym="priS"
     CDS             7345..9078
                     /gene="hcp"
                     /locus_tag="PLM74_02555"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019879115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HON41999.1"
                     /translation="MFCYQCEQRAANGGCRVMGVCGKDPQAAALQDLLIRASQGISMY
                     AHRARALGASDREIDLFVVEALFTTVTNVNFDPERLADLVRRAAQLRDKARDMYTHAL
                     AASSVAWDAGSGATSGATSGAASGQPAQAFQPAPDLGGPATFAPADDLAGLVKQGEEV
                     GAAWMARSIAVGADAAGVVNLALFGLKGMAAYADHAQILGVEDAEVYGFFHEAMDFLA
                     RDHFTLAEALEMALRVGEVNIKVMAMLDKANTTAYGNPTPTRVRVTPVKGHAILVSGH
                     DLADLEAILQQTEGKGINVYTHGEMLPCHGYPGLKKYPHLVGNYGGAWQDQRREFAEF
                     PGAIIMTTNCIQKPQDSYRHRIFTTGLVAWPGVRHIADRDFSPAIDAALAEPGFSEDE
                     PDHYITVGFGHNAVMGVAGQVIDAVKAGKIGHFFLIGGCDGAKSGRNYYTELAQRVPD
                     DCVILTLACGKYRFNKLDFGEVAGIPRLLDVGQCNDAYSAVVIAMELAKAFGTDINGL
                     PLSLVLSWYEQKAVCILLSLLHLGVRNFRLGPTLPEFLTPSVVKVLQDKFGIMPITTV
                     DQDLVAILGQA"
     gene            complement(9697..10776)
                     /locus_tag="PLM74_02560"
     CDS             complement(9697..10776)
                     /locus_tag="PLM74_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012749070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HON42000.1"
                     /translation="MKKVMQMFITVFIAAFALVGVTSYLNSSNGYSGADTLTVYNWGD
                     YIDPDLIHKFQRETGIRVIYETFDSNEAMLTKIAHGGTAFDIVIPSDYAINKMKAEGM
                     LIPLDHSKIPNLDNIDPRFLDLPFDEGNTYSVPYFWGTMGIIYNPELVPGKEFSSWAD
                     LWDEELGSEILLVDSAREVIGMGLNSLGYSLNDIDTEHLQEAYSKLCELTPNVKAIVG
                     DEIKMLLANEEAAVGVVWSGDAAIIMDENDKLDYVIPSEGSMVWFDNIVIPTTARNIE
                     GAHLFINFMLDPEIAAQNTEYVGYATPNAAALEHLPEEITDDERFYPDPDVTDNLEVL
                     ANLGKRMLGYYNELFLQFKMHRKRD"
     gene            complement(10773..11597)
                     /locus_tag="PLM74_02565"
     CDS             complement(10773..11597)
                     /locus_tag="PLM74_02565"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019005694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HON42001.1"
                     /translation="MSSNSKKTGKGITIYLMVVFFILYAPILYLAYYSFNSGGAMHSF
                     EGFTLEWYGEVFQDTRLVIILMNTLAIALFSAAISTMIGVAGAIAITHVRSKQFRSAL
                     LTLNNVLIVSPDVIIGASFLILFAAMGVKLGFASVLISHIAFSVPIVVLMVLPKLQEM
                     SPTLIYAARDLGATRWDVLTRVIVPYIAPGIFAGFFMALTYSLDDFAVTFFVTGNGFS
                     TLSVEIYSRARQGISLTINALSTLIFMVTLVLAVGYYVMNTRATTPGRPARVEAGE"
     gene            complement(11599..12399)
                     /locus_tag="PLM74_02570"
     CDS             complement(11599..12399)
                     /locus_tag="PLM74_02570"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003253139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HON42002.1"
                     /translation="MQYRTRNIYLIPYAAWIVVFVIAPIVLIAYYSMFDIDGALTLGN
                     YRRFFTPVYMGMAVRSFWYAFLVTLFSLLVAYPTAYLLTKTKHKQLWLLLMIMPSWMN
                     LLLKAYAFIGMFGTYGPVNALLGAVGIGTKQILFTDFSFIFVAVYIFIPFMILPIFNA
                     LDELNPSLVAAARDLGASGWDTFRRVIFPLTTSGVKSGCRAVFIPALSLFMITRLIAG
                     NRVITLGTAIEQHFLVTQDWGMGSTIAVFLIIAMGLTMVLTGSGEGGR"
BASE COUNT         2678 a         3767 c         3493 g         2492 t
ORIGIN      
        1 cggcgcgcac gcgccggcga cagcccgttt acctcgacaa gccccgatgt gggatgcaga
       61 atcccggtga gcatcttcac ggtagtcgac ttaccagccc cattcggtcc gatgtacccc
      121 acgaactcgc cctgggagat atcgaagctg accccgtcca cggcgatcac atcacgggtc
      181 tcgcacgcca ccagctgacg tatgctgccc caaagaccgg gtttcctcac cgccacacgg
      241 tataccttgc gcagcccgtc cgccatgacc gcggtggccg cccccacact gctcatagcc
      301 catcacccct tccgcatcga gaatcgcaag ttgcaggccg caactcgcct cgtgcttggc
      361 atctcggttg ccgtctgccg cgtgagccgc ggcaagccct ctacgtccca gtgcttgtgt
      421 agtgcgcctc ccctgtcttc caaagcttga gtgccgccca tactgccact gggctggcga
      481 gtgccggcca gagtaggttg agggccgtgc cgcccttgtc caggatgaac ctgagcggaa
      541 cgtagcagcc aaggcccagg ggcaacagcg tgacgaagac ccacttgagc caggatggat
      601 acacgtccag tggatatgca gtggcggtag acggcgcgtt catcgtgaac acctgcagtt
      661 cctcgatgcg aacgatccag aatcctgctg ccgccgtggc aagacccaca gcaaagtaga
      721 gcagcgcgct ccatgccacc gaggccagca gccagagagc gtcccatcca ccgattactc
      781 ctgtcgacac aagtcgcggc gccgcaagag ctgcagctgc tatcccctgg gcgagaaacg
      841 caagacgatc tatacccacg ccccgcgcca acagggtaaa gagcgtgggc cacggcctga
      901 tcagcagtgt gtcgaaatcg ccgttgacca tgtacagctc gaatctgtcg agctcgttgc
      961 agaacaacat gtatactccg tttcccatgc gggaaacggc atacagcatc gcgatatcga
     1021 agatgctcca gcccatcacc ggcccgaacc gccacagcac cgcgcctatg aacagaatgt
     1081 tatctgcccc catgagggtc tggatgagag tgctcccgag gaaatccaac cggtactgca
     1141 tcttccctct gaggcttgcc gagaccagta tctcgtacgt ccgcgctgcc cgacgaagcc
     1201 tcgtcgccgc agtcgctact ataccgggcg ccccagccga cggtcgcgca caccttcgtt
     1261 ccatcatctc ccctgcacct ccagtcgccg gcgtgctcgg gacgtgacac ggcgagccac
     1321 aatggtcaga acagccgccc acgcaagtcc cgcggccaag atgggcgccg gtccggaaag
     1381 ctccaggtag atgcgtgcag gatagtaggc cagatgagcg aagggtgatg ccttagcgag
     1441 aagctccagc ggacgaggca gcacctcaag cggcatagac agcccgccca acaaagcctc
     1501 cagggagaag agcacccatg acaacgtgcg tatctcattg gtccagaaag acgatatgcc
     1561 gaccaggtag ttcagggaaa tgccaatgta cccgccgatt gcgagcgatg ccagggcccc
     1621 gagccatgtc gaggctgatt caggtagacg cagctgcgcg agcagtgaca gcacaagccc
     1681 cactgcaagg ccgcggaaca tgaagctgta aacctgcccg ccatactcga aggacatatg
     1741 gtaccagaag aaatccacgg ggcgtgccag ctccaccgca accctgccgg acctgacaga
     1801 ctggaccatc ctctggcgta tcttcatgcc gaactgcgtg aaccatgcag tggtttggtt
     1861 gatgcatatg tactgggcga tcatggacga ggaatacgcg ccgaaaccgg attcgggcgt
     1921 caccgcccgc cacacgtata tgtagatgag gccaaaaact acactggcga ttgtgttcac
     1981 tatatgagac gccaagtact gaagattctg tgcaaacccc ttgcctgcca gcacccgcca
     2041 ctgcctgacc gcggcatgca ctttggaacc cccctgcccc tgctctgctc cgattgagcc
     2101 ctcgtccggc cactgccgca cgaagcctct acgtccagcg cgttcgtcta caatatcaca
     2161 gtgtgccggg ggctcgcaag cacccagcgc catagcatca cagcatcatt cgctctgccg
     2221 ctctgcgctg tgcgctctgg tagggcgtac gttgcggtcc ggtagtagcg gtcagtcggg
     2281 tcaatcgggc cgatcggggg gctacggtat ccccgcccac atacccccgg gggtatctag
     2341 gcggggatac cgtcaagttt tgacgatagt ttgggccgat gtactcaatt tttgagtacg
     2401 tccagttggg tcgctggaca gccggggttg ccaggggcct attcggggcc gatggtaccc
     2461 tattatgtgg gtaatcgcta gcaattagag ctatggccga cacgctgcta ccaaaactgc
     2521 ccacattatg gggtatagtg ccactcgccc taccggttct ggtgccctag tatgccggat
     2581 accccccggt ttccgtaatc cgggggtggt cagactgcca tccgaaatcg atgtactcaa
     2641 attctgaggg tatctccaaa actaatgtca aaaacggagt acgtgccagc tacagccgtt
     2701 ttggcgtcct actaccctca ttaggccagc ttggcggcat ttccggcgag cgcatcggtt
     2761 gttactcccc gagatgcgcg gacctatcgt gaaggccaac gtgtggcagg cagatgaagt
     2821 cctgttcctc aaaatgggca tcgaacgaaa aggctgtatc gatgccgagc acccgcatga
     2881 cctcaaagct tacacaatcg acgacactta gccccctccg attggcagtg acaacggcag
     2941 tcacggcggt ttcatgcata cgcttatcaa cccactccac ctgaagcacc ggatagacgg
     3001 acttccgaaa gcccctaact gcgtcaaccc ctagccggtg ctggatcaga gcgcacgtct
     3061 caagcaggac gtagttgctg cacacaagtg gtgtatcgcc ctccaagagc tcgacccaag
     3121 tgctgcgagc tgcacagtgg ttggcatcgt cagcatcaat caccgcatag agcgcggacg
     3181 tatctgcgta cacgctcatg cgtcatactc ctcctcaaga tatgcatcgt ggttgacgga
     3241 tacatcgctc ttccctgatc tgaactggcc ggctatggca atagctcgcg cgacctgctc
     3301 ccttcggttc atggttcgcg cctgcccaat gaccatgtct gcgcccctgc ggacgagttc
     3361 cgaaatcgat actccctcgg aagcagccat ggcacgtaag gcctcaacct gctcctcggt
     3421 gagctgcacc tgcatgcgaa ccacgtcttt tccctccccc acgccatctc cgaagccatc
     3481 ggtgcgacac tttggtctca ataccatcat tatatcacaa gtgatgtcat atgccaggcc
     3541 cggcatgatg tcacgttaac cttagcctag cccgtcgtcc aaggtttgcc cgctgcatcg
     3601 gcgatggcag atttcagtcc atcctggtcc gagaactgac cgtcggcgaa cagctcgact
     3661 agttccccac tgcctaggcg cgccaggaaa gcggtgtagg ttgtcctacc cgatttgacc
     3721 cgtcgctgct ccagcgattc cacgcccgat aggtcgaccc tcttcgatga tgttcctttc
     3781 accaattcga ccatgttccc atcgactact atcttcctgt cggccattga tcttgccgct
     3841 ctccaggcca aggccgcaag cacaacccaa gtacccacta ctacggccgc gcctactccg
     3901 cctgcggcga tttcgcccca gaacatgacg accatcacaa tcgcgataag caacactgcg
     3961 acgattgttg gaccagtcgg atccttcggc gtgaacacct tcaatgcagt cactccttca
     4021 tcaaatgcct cgctagctca cagtttacca ccgcagacca tcgtctacgt ctcctgatat
     4081 tgccatcagt cattcgacaa agcgcgcagc actcctctat gcgctcttga ccatgcgctc
     4141 cagggtgttc agctacgttc ggttgttcgc cgatagactg ctgagcatag gctgattgac
     4201 gccccagatg aaaggaggac ggttcccaac gcccaactgc cgcggcaggt gcacgccatc
     4261 cgcgcgtcga agatgtaagg atacggagga gggatcgctc atgctagagg gtgccctcga
     4321 ctatcctgag gataaggctc aactgtacgt cctcttgcag acggaactgc gggccctggt
     4381 cgaaggagtg ccgcaaccga taccgaacct ggccaacgcc tcggcgctac tctggcaggg
     4441 tctgcggaat ataaactggg ccggctttta cctgatggac gacgggcagt tggtcctagg
     4501 gccttttcaa ggcaagcctg cctgcatata cattcccgtg ggcaaaggcg tgtgtggaac
     4561 cgcagtggaa gaaggtcgag ccttgcgggt ggacaacgtg catgacttcc cggggcacat
     4621 cgcgtgcgac gccgcatcga attcggaaat cgtcatcccc atccgcgacg gcgagagggt
     4681 cgtcggtgtg ctcgacatcg acagcccgct cctagcccga ttcgatagcg aagatcagga
     4741 tggactggaa gggatcgttc gaatcctgga acgagcctgc gtgtggccgt agtatccaga
     4801 cttcagctga ggaggaatcc atccatgtca attcgcagag gcataatcgc cttcgtgcta
     4861 cttctatgta ttacggtgtc gtctgccaca gccatgggcc agatcgtcac ccctgagcag
     4921 atgctgaagc agatcttcac cgccgaagtt atgtcagagg actggttttc ggcggctttc
     4981 ctggcccagg tacctgtggg ccagctgagc gagatcgtca ggcagtatcg tgcagccctg
     5041 ggcgcacttg aagacgtaca aggcaaggca cccgcattca ggctcacatt cagccacggt
     5101 tacgttgccg cacagatcgc tttggacggc gcagggcgcg tcgccggctt tttcctcggc
     5161 ccgcccgagc cacgcgtaag cggactggat aacgcccttg acggtttccg caaactgcct
     5221 gggagagtga gtgtgctcgt cgtatcgcag tccggcgtcc ttgcccagct caaccctgaa
     5281 cagcccttgg ccgtcggctc agcgttcaag ttggcaatcc tggcggccct gaaagatcgg
     5341 gttgcggccg gcgctctagc ttgggacgac gttgtgaccc ttgaagcaag ccacattagt
     5401 ctgccgtcag gcatcctcca gagctggcct gtgggatcgc ccttgacggt gcatacgctg
     5461 gcatcgctga tgatctcagt cagcgacaac acggcaacag atgccctgat cgcactgctg
     5521 gggcgcgagt tgatcgaaga atacactgcc ctaaaccacc ctctgctcgc tacgcgcgaa
     5581 gcatttgccc tgaagaaccc ggcaaatgca gaccacctca aggcgtaccg cgagggcgac
     5641 atggctcagc gcagagaggt gctgaagcag ttgcagtccc ttcccctccc cggcgtcgct
     5701 gacttcacca gccctcaggc attagacgtc gagtggttct tctcagcaag cgaactagcc
     5761 aacctcatgg cggctgtcca ggacctatcg gtgttcagca ttaacccggg tgtcgcttcg
     5821 cctgccgact ggcagcatgt cgccttcaag ggcggctctg agcccggggt gatcaacctc
     5881 accacctggt tggtcgggca ttcggggatg aactactgcg tcgtggctac atggaacaac
     5941 gatgaggtcc tcgatgaagt gcagtttatc ggcttgtaca gcggaattat ccgacagttg
     6001 gcgcagatag acgccaaacc gtagcgaaag gcaccggata cgagtcatcg tcggaactca
     6061 cgcagaggca ccacctctcc tctggccacc tctgcatctg cttcagcaag cgctctctct
     6121 ttatccgggc tcagtgccac tggcgggcgg ggcttgcaga gctttagcgt aggaaaggag
     6181 ttctgcgagc ttcgcttcgt ctgcgatcga gacaacacgt atccatgcag gtgagtccat
     6241 ttgaatgaca tattgtgcag gaaggggtcg ttctctgtgg atggcgccgc cgccctccct
     6301 cgcatgccct cgcacatttc tattgactga tattgaccta ttgtggtacc ttaataccag
     6361 aatacctttg tagaattgtg gactagactg ctggcggccg tgggcagtac cacagccagt
     6421 caaacaaaag gacaacgttg ggaaaggatg tagagcacca tggatcggca gacaattgcc
     6481 atcgatgaaa gcaagtgcga tggctgcggc gcctgtgcca gagcctgtgc agagggagtc
     6541 atcgagatta tcgacggtgt agcgcgcctc gtgaatgagg atcactgcga cggactggga
     6601 gcgtgcatcg gcgactgccc caacggcgcc atatctttgg tcacggttgt ttgtgaaccc
     6661 gcgaccgccg aagccgccag tggcggcccc gccgatgggt ctagcgggcg gcagaccgct
     6721 ggttgccctc tgtggagtat ctctttggat agtgtgagca ctgacgcggg aggccaggaa
     6781 accgcgaaat ccccgtcgcg tgcagtcccc aactggccca ttcaggtgaa cttggtctcc
     6841 cccactgctc ggtggctggc aggatcggac ttacttgtgt gcgccgactg tgtcgcatac
     6901 acctgccctg acttccactc aaggcatctt cccggcaaga cgttgctggt gggttgcccc
     6961 aagtttgatg accgcgagcg cttcgtatcg cggttggctg agattctgtt ggtagcccaa
     7021 gtccggtcca tcgaagcgtt gcatatggac gtggcctgct gcagcgggct cggccgtctg
     7081 gtggaagagg cccaagagag agccggaacc caagtgccgg ttagcacaac tgtagtggga
     7141 gtggacggca gagtgctgta gataccgaaa gcagcgcagc cggcctaggg cgccgcagct
     7201 tgctggcacc acgcataagg cggggaacgc cgcacagccg agagtatccc cgtggcgggc
     7261 tcgtgagcct ccagtgccaa ccccggcggc gcttttctga atacaccgac ttctactgac
     7321 aatcaacaaa gggaggattg ccaagtgttc tgttaccagt gtgaacagag ggcggccaac
     7381 ggcggctgcc gagtaatggg agtatgcggc aaagacccgc aggccgcggc actccaggac
     7441 ctactcatcc gcgcgtccca gggaatctcg atgtacgccc accgcgcgcg agcgctgggc
     7501 gcatccgacc gcgaaatcga cctgtttgtg gttgaagcgc tcttcaccac agtcaccaat
     7561 gtcaacttcg accccgagcg cctagccgac ctggtccgcc gagccgccca actgcgcgac
     7621 aaggcccgcg acatgtacac ccatgcgctt gcagcgtcga gtgtggcatg ggacgctgga
     7681 tcgggcgcta cgtcgggcgc gacgtcaggc gcggcgtcag gccagcctgc acaggctttc
     7741 cagcctgccc ccgacctcgg cgggcccgcc acatttgccc cagcggacga cttggctggc
     7801 ctggtcaagc agggcgaaga agtaggcgcc gcctggatgg cccgctcaat tgccgtaggc
     7861 gccgatgccg ccggcgtggt gaacctagcg ctgttcggcc tgaaggggat ggccgcgtac
     7921 gccgatcacg cacagatact gggggtcgag gatgcagagg tctacgggtt cttccacgaa
     7981 gccatggact tcctcgcccg cgaccacttc acactggccg aagctctgga aatggccctt
     8041 cgcgtcggcg aggtgaacat caaggtaatg gccatgctcg acaaggccaa taccacggcg
     8101 tacggtaacc ctacgcccac gcgcgtgcga gtcaccccgg ttaagggcca cgccatcctg
     8161 gtatcaggcc acgatctcgc ggatctcgag gccattctgc agcagaccga aggcaagggc
     8221 atcaatgtgt acacccacgg cgagatgctg ccatgtcacg gataccccgg tctcaagaag
     8281 tatccccacc tggtgggcaa ctacggcggc gcctggcagg accagaggcg cgagttcgcc
     8341 gagttccccg gggctatcat catgaccacc aactgcatac agaagcctca ggactcatac
     8401 cggcaccgca tattcaccac cggccttgtg gcgtggcccg gtgtgcgcca catcgccgac
     8461 cgcgacttct cccccgcgat cgacgcggcg ctggccgagc ccggcttttc ggaagacgag
     8521 cctgaccact acatcaccgt tgggttcggg cacaatgccg tgatgggtgt ggccggacaa
     8581 gtgatagacg cggtgaaggc aggcaagatc ggccatttct tcttgatcgg cggatgcgat
     8641 ggagcgaaaa gcggcaggaa ctactacacc gaacttgccc agcgcgtccc ggacgactgt
     8701 gtcatactca ccttggcctg cgggaagtat cgcttcaaca agctcgactt cggcgaggtg
     8761 gctggcattc caaggctcct cgacgtgggc cagtgcaacg atgcttactc cgcagtggtc
     8821 atcgccatgg aattggccaa ggccttcggc accgacatca atggacttcc cctgtcctta
     8881 gtgctgtcgt ggtatgagca gaaggcagtg tgcatactcc tctccctgct tcacctggga
     8941 gttaggaact tcaggctggg gcccacactc cccgagttcc tcacaccctc ggttgtaaag
     9001 gtgctgcagg acaagttcgg catcatgccg attacaaccg ttgatcagga tctggtcgcg
     9061 atactaggcc aggcctaatc aggcggcgaa cagtctgcgc cccatgcgga agcatcgaat
     9121 ggcgagtcta tggacagcct agccggggat tgctccgata aagaggcctt gcgcaggccg
     9181 ttagggaaac ccgcatataa cagggactta gcgaacctgt aagaaatgtg actggttttc
     9241 tagtgaccct gctcaagatg tgtcaggttt gaacaccgac ggagctaagg ggtggtcgga
     9301 cttggatgct tctagtatat cggcaggcat ttgatgcctt cccagacgcc ctcgggaggg
     9361 tcacgcacat ggccatatta tgtaacccgc ctctgcctgc ggctttgctg gctgtctggc
     9421 tccagaaatc ggaaacgccg tggccggcgc gaattctgac aactcgtgcc ggccaaacct
     9481 ggcagaatcc gagcaggtcg ctcagagaac cggccacaaa acgagcagga taggccagca
     9541 ccaaatcagg ctaaccaaac tctgtaagga ctaaccagcc tccgtaaaca cgatgcacat
     9601 cttttccatc gctgtgggta atgcatagtg gaaagcccac aggtgatagg aacaccacgg
     9661 ggtctcatgt gaatacttgg ggtctcccga ccgtccctaa tccctcttcc tgtgcatctt
     9721 gaactgcagg aacaactcat tgtagtagcc cagcatccgc ttgcccaggt tggccaggac
     9781 ctctaggttg tcggtgacgt cagggtcggg gtagaacctt tcatcgtcgg ttatctcctc
     9841 cggcaggtgc tccagagccg ccgcattcgg tgtcgcatac ccgacgtatt cggtgttctg
     9901 cgcggcgatc tccggatcaa gcatgaagtt gataaacagg tgcgccccct cgatgttcct
     9961 ggcggtggtg gggataacga tgttatcgaa ccataccatc gatccctcgc tgggtatgac
    10021 gtagtccagc ttgtcgttct cgtccattat tatagcggca tcgcccgacc atacgactcc
    10081 aacagcagct tcttcattgg cgagcaacat cttgatctcg tctcccacaa tggccttcac
    10141 attgggcgtg agctcgcaca gcttgctgta cgcttcctgc agatgttcag tgtcgatgtc
    10201 gttgagcgaa tatcccaagc tattcagccc catgcctatt acctcacggg cgctgtctac
    10261 cagcaggatc tcacttccca gctcctcatc ccacaggtca gcccagctgg aaaactcctt
    10321 ccctggaact agttccgggt tgtagataat gcccattgtc ccccaaaagt aagggacgga
    10381 atacgtgttg ccctcgtcaa agggcagatc aagaaagcgc gggtcgatat tgtcaaggtt
    10441 cgggatcttg gaatgatcaa ggggaatgag catgccctca gccttcatct tgttgatggc
    10501 atagtccgag gggatcacga tgtcaaatgc cgtacccccg tgggctatct tggtcagcat
    10561 cgcctcattg gagtcgaatg tctcatagat gaccctgatg cccgtctcgc gctgaaactt
    10621 gtggatcaag tcgggatcga tgtagtcccc ccagttatat acagtgagag tgtccgctcc
    10681 tgagtagccg tttgacgagt tcagatacga cgtgacgccg accagggcga atgccgctat
    10741 gaataccgtg atgaacattt gcattacctt cttcattcgc ccgcctccac tctagccggt
    10801 ctgcctggtg tggtcgcgcg tgtattcatc acgtagtaac cgacggccaa caccaaggtg
    10861 accatgaaaa tcagcgtcga cagcgcattg atggtcagcg agatgccctg ccttgcccgc
    10921 gagtagatct ccaccgatag cgtggagaag ccgtttccgg tgacgaagaa agtgacggcg
    10981 aagtcgtcca gcgagtaagt tagggccatg aagaagcctg cgaagatccc aggcgcgatg
    11041 tatgggacta tcacccgggt caacacgtcc cacctggtgg ccccaagatc gcgcgctgcg
    11101 tagatcaatg tggggctcat ctcctgcagt ttcggcagaa ccatcaggac gacaataggt
    11161 acgctgaagg cgatgtgaga gatcaacacc gacgcgaaac cgagcttgac ccccattgcg
    11221 gcgaacagta tcagaaacga tgccccgatg atgacatcag ggctgactat caacacgttg
    11281 tttagagtca gcagtgcgct cctgaactgc ttgctccgga catgtgtgat ggctatcgcg
    11341 cccgcgacgc cgatcatcgt ggatatggcc gcagagaaca aggctatggc tagagtattc
    11401 atgagaatga tgaccaggcg cgtgtcctgg aacacctcgc cgtaccattc aagcgtaaac
    11461 ccctcgaagc tgtgcatggc cccaccgctg ttgaaagagt agtacgccag gtagaggatg
    11521 ggagcgtaca gaatgaagaa gaccaccatg aggtatatgg ttatgccttt ccccgtcttt
    11581 ttcgagttac ttgacatgct acctgccccc ctccccgctt cccgtcaaca ccatagtcag
    11641 gcccatggcg atgatcaaga acacagcgat ggtagaaccc atgccccagt cctgcgtcac
    11701 aagaaagtgc tgttcaatgg cggttcccag ggtaatcacg cgatttcccg caatcaggcg
    11761 cgtgatcatg aacagcgata gcgccgggat gaatacggct cgacatcccg acttgacccc
    11821 gctagttgtc agggggaata tcactcggcg gaaagtgtcc caccccgatg ccccaaggtc
    11881 gcgagctgcg gcaaccaacg acggattgag ctcgtcgagc gcgttgaaga tgggcaggat
    11941 catgaatggg atgaagatgt acactgccac gaatatgaaa ctgaagtcgg tgaacagaat
    12001 ctgcttcgtt ccgattccta ctgcgccgag cagagcgtta acagggccgt aggtcccgaa
    12061 cataccgatg aaggcgtacg ccttgagaag caggttcatc catgacggca tgatcattag
    12121 aagcaaccag agctgcttgt gctttgtctt tgttagcagg tacgctgttg gatacgcgac
    12181 caacagcgaa aacagcgtga ccaggaatgc ataccagaac gacctgactg ccatgcccat
    12241 gtagaccggt gtgaagaacc tgcgatagtt gcctaacgtc agcgctccat cgatatcgaa
    12301 catggagtaa taggcgatga gcacgatggg cgcgatgacg aagacaacga tccacgccgc
    12361 gtatggaatt aggtatatgt tacgtgttct atactgcata gcgcgcctct tcatacgatt
    12421 cgatacgctt
//
LOCUS       DAOTAC010000034        12344 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_4336_length_12344_cov_4.436651, whole genome shotgun sequence.
ACCESSION   DAOTAC010000034 DAOTAC010000000
VERSION     DAOTAC010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 12344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12344
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4336_length_12344_cov_4.436651"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1094)
                     /locus_tag="PLM74_02575"
     CDS             complement(<1..1094)
                     /locus_tag="PLM74_02575"
                     /inference="COORDINATES: protein motif:HMM:NF014598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HON42003.1"
                     /translation="MNSLLVMVVTFALYILAYNLYGKKLGSKVFELATRNITPAEKHN
                     DGVDYVPTNKFILAGHHFTSIAGTGPIVGPVLGVIWGWVPALLWVVFGSIFAGAVHDF
                     GALVLSMRHDGRSIGDITGSVVNPRTRSLFFLIIFFCLWIVIAIFAILMANLFVQYPQ
                     CVFPIWIEIAIAMGLSRYLYEKKGSLLIGSIVAIVLMYLTIWIGLYIPVALSKATWII
                     IIMIYIFFASTMPVQRLLQPRDYINALELGVAMVLLIVGIIVRPLPIIAPAVVSSPAG
                     APSMFPFLFITIACGAISGFHSLVSSGTSSKQIDKEPSALAVGYGGMLLEAALATLVI
                     IAVTTGVSKEAWLTTYANYAKVPTLPTFAEG"
     gene            complement(1254..2153)
                     /locus_tag="PLM74_02580"
     CDS             complement(1254..2153)
                     /locus_tag="PLM74_02580"
                     /inference="COORDINATES: protein motif:HMM:NF040786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism-associated LysR family
                     transcriptional regulator"
                     /protein_id="HON42004.1"
                     /translation="MDIRQLAIFVSIANHGTFTAAGKALYISQPTVSVQMAALEKELG
                     VALFERQSRGIALTPAGKILKRYAEDILMLKDQAVAAMGRYKHDVSGRLRVIASTVPA
                     DYILPGVVSRFLHAYPGVFVELSRADSATVWDAIRNYEAELGVAGSAREDSSLDHLAV
                     ARDELVLIAPFTEEYSHWEDPVPLESVLRAPMLCREPGSGTQKTFDDALRLLGVDPER
                     ISVRAQLDSVEAIKSAVAEGGGVAVVSSLAMQGDTTAQSIRILHIEGLSLVRCFYLIT
                     HAKRVLSPAAQAFRDFVVSSLTC"
     gene            complement(2182..3459)
                     /locus_tag="PLM74_02585"
     CDS             complement(2182..3459)
                     /locus_tag="PLM74_02585"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HON42005.1"
                     /translation="MKNRARALIKGFRGDKYAYGDGALGRVGAYADELGGRRALVIAN
                     RSAWLEPIASQVVSSLGQSGIELAGGRIAPGSRPNSLVDDVYRMALYIVLFKPDCIVA
                     VGGGSTIDAAKAANVVACLGHDDPELEGYYGMGLVGPALTRTGAKLLPMVAVQTASGS
                     GAHLTKYSNVTDAWAGQKKLIVDEIITPDRAVFDYSVTRSAPPDVTLDGAFDGLAHCL
                     EVFFGAKGANLGLLQEIAEVGIELLVSSIPAVMADPDAHEPREALGLATDLGAYAIMV
                     GGTNGPHLNSFSLVDVTSHGRACAVMNPYYAVFFAPAIEGKLRLVGDIFRRYGYIAQD
                     LDALSGRELGIAVAEGMTNLSRKIGFPTRLSELPGLTDEHISRALGAAKNPQLESKLQ
                     NMPVPLVASQVDKYMGPILEAAKSGDFSLIVNM"
     gene            3631..4425
                     /locus_tag="PLM74_02590"
     CDS             3631..4425
                     /locus_tag="PLM74_02590"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HON42006.1"
                     /translation="MLEQVKCLILDLDGTVYLGDQLIPGALLLRQAAQDFGIDVFFLT
                     NNSSRSRSYYASKLTRMGWPVTADGVLSSSQATAIVLKNRGYQRLCVVGTDSLTEELT
                     AYGLTVCGDEEEHVDCVVVGFDTSLVYGKLRAAGRHLQRGTPMISTNPDLVCPLEGGE
                     FIPDCAAISACLMTAYGVATEYIGKPERGMLDLVEANTPYRGKQIAMIGDRLYTDVRF
                     GSRWGLLSVLTLSGESSLSDLGPDDHPDLIVDDVGQLAKMIRQARS"
     gene            complement(4410..5018)
                     /locus_tag="PLM74_02595"
     CDS             complement(4410..5018)
                     /locus_tag="PLM74_02595"
                     /inference="COORDINATES: protein motif:HMM:NF016216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HON42007.1"
                     /translation="MEWTPGLSQRDRERIWQHLAKHPAIPALKRVEDVDAAHRYRAKL
                     VFFLTGTLYDLRDVCARGDCLGMQVFCHLDLVQGIGKDPAGIKWLATEIGVRGILTTR
                     STLIRAAKNEGLMAIQRLFMLDSESLKTGLEIIASSRPDAVEILPALVVPSMAHRLPM
                     DKVPPFIAGGLVETVDEIESIMASGALGVSTSKKELWAYERA"
     gene            5055..6266
                     /locus_tag="PLM74_02600"
     CDS             5055..6266
                     /locus_tag="PLM74_02600"
                     /inference="COORDINATES: protein motif:HMM:NF025418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HON42008.1"
                     /translation="MRRLQSMILVTSVVIAATLGAVTYAAPDVPNCPLHVDGGLLTIV
                     YDSPDSAPALSVEYDGLQLLLVHAVEPFGPFEQFGSSRRLELFAGAGLPKWGPGRTGS
                     LMMSGRTAPLPHVGYHLAVGRADYVRFVGMMESEQRRRLLGHRLEYSPTPWLHLGVSE
                     TAVVSGEASPLLYWPFPGLPLYALQRAVSQRDIAQDSLINVNFGADFVATLPGEGRRP
                     ELQAYGELLIDDAQGTMSDRDSVPDFLGGLVGVRVPRFTSDPRFSAYLEYVAISSYVY
                     SHRNPDNNYVYRGTIIGHSLGPDADMLAVVTDFRPDSKTAVSLTGAVVRHGESEIGHP
                     WSPAEGRDREFPSGIVEKTLKASLEVERMLLEPVLIVGRLELGTTFNKGHVQGSTQRY
                     WSAAAGIGVRL"
     gene            complement(6290..6727)
                     /locus_tag="PLM74_02605"
     CDS             complement(6290..6727)
                     /locus_tag="PLM74_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42009.1"
                     /translation="MKKTTALVTLVLLCTVALAMLPAQASAAGSAWPHLDRAQVSFIL
                     ADGVSAGLGASLSQYLRLAVNLDIRRGPGSAGASVLLVYPRQFTILTCLYGGVGLTRE
                     FAPDSTGLHLVGGGEFGAWFAEFEWLIPPRNYGKLRSGLRFVF"
     gene            6774..7241
                     /locus_tag="PLM74_02610"
     CDS             6774..7241
                     /locus_tag="PLM74_02610"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HON42010.1"
                     /translation="MRDANGPKADKYSESWAALWWLLALAYPVLLIVAASRPEFGAEF
                     TQMLVKRLLPGLAGRQVHVLVVAIRKSGHFLGYSLFATILVNAFLSFSRPPRNRRTVV
                     VAYLAAAIAAVGVAAIDEYIQVSAAFRSGSRQDVLLDSLGVLAALVVRIRRPE"
     gene            7379..8398
                     /locus_tag="PLM74_02615"
     CDS             7379..8398
                     /locus_tag="PLM74_02615"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HON42011.1"
                     /translation="MTLRKAKFAALTILVGVFALLAACPGSALAAHLIVTLTDGSQVT
                     YNIPVDESNVQSVALGSSQQSMTDAEAAIATVNGQPITRQDFYIALSEAAGRPVLDQL
                     ISEMLLAQAATEAGVSVSNEEVDREFESVRQQVGPEFESLLAQYGMTADDLRKNIKIS
                     LLVFRVSTKDVVVTDEQIAAYYNEHTADFESPEMVKASHILVETEADAQAALDRISAG
                     ADFADVAAAVSMDPMTAQNGGDLGFFAAGEMVPEFGDAAFALKTGEISGIVKTDYGYH
                     IIQVTDRVEPRRASFEEASGVIERLLRSAHAMKPADLIAQLKETSLITVFDSRFTDLG
                     TIPLP"
     gene            8412..9137
                     /locus_tag="PLM74_02620"
     CDS             8412..9137
                     /locus_tag="PLM74_02620"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HON42012.1"
                     /translation="MLRTVLFDLDGTLVHFEFDDFMKAYLGAISNKVAQLVDAKVFVA
                     QLLASTEEMVRSLDPARTNHDVFADHFFSGLGRTEDELMPVFDAFYDEDFPRLRDALG
                     VEPHPLARPMIQGLIDRGYEVVIATNPVFPANAIEERMRWGGLDGIPYRHVTSYETSH
                     FCKPNTEYYAEILDVVGRSPHECMMVGNDTCEDLVAGELGITTYLVEDYMLDRGPYRA
                     VPDHRGRFEDLARFLGSLGCVGA"
     gene            complement(9182..9595)
                     /locus_tag="PLM74_02625"
     CDS             complement(9182..9595)
                     /locus_tag="PLM74_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014259310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HON42013.1"
                     /translation="MSHERPTVGGEFDLIVTIINKGWADEIVEAARSAGAEGGTVLYG
                     RGTGVHERKSFFGILIEPEKEVVLTLIPRERTQSVLQAIIKAGQLERPGTGIAFVLDV
                     SQVAGIIHLANPANPASPAAPASPADSGGASVGSE"
     gene            complement(9547..10362)
                     /locus_tag="PLM74_02630"
     CDS             complement(9547..10362)
                     /locus_tag="PLM74_02630"
                     /inference="COORDINATES: protein motif:HMM:NF019194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HON42014.1"
                     /translation="MDRIVVFHGFGQVMAEVARTLIPLGALFTVFQVFYLKLPRTFVR
                     RVAVGIVWTFIGLSFFLQGVNIAFVPLGRALGAQLGKMDNPIAVLPVGFVLGLLVVLA
                     EPSARVLSGEVEKASAGHIRERLLLGTMALGVASLVSLGYYFVARGRPLTHIIIPGYG
                     VALALLRWIDPAFAAISFDSGAVATGPLTVTFLMSLGLGIAAQQPGGPTIGSGFGLVC
                     LIALASTLAVEGLSFAFRAKKDIAVPGEADTSDGGNSICHTKDQPSVGSSISS"
     gene            complement(10362..11054)
                     /locus_tag="PLM74_02635"
     CDS             complement(10362..11054)
                     /locus_tag="PLM74_02635"
                     /inference="COORDINATES: protein motif:HMM:NF019194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HON42015.1"
                     /translation="MSRQFRAAVSEVVNAVAPLTLAVAILSTVFLGLSWVMLAQLLVG
                     AVLTTAGLFLFLRGTRIALLPMGEMIGAYLPRRGSTALLVATAFIFTFSVTIADPSVH
                     VLVAQVGSFPDSGINPQVLAVLLSGGMGLLATIAILRILLNIPIKWLFAGAYAAVIVL
                     SAFTHPQLVPLFLDSGSVSTGPMAVPCIIALGLGTASVLQRKQTLADEFGLVGLATLG
                     PILVLMVWGVFM"
     gene            11302..>12344
                     /locus_tag="PLM74_02640"
     CDS             11302..>12344
                     /locus_tag="PLM74_02640"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HON42016.1"
                     /translation="MLSHSVLPVGRPGKLWAVVRLSSGSVQQGAAERLPLNVGVVLDR
                     SGSMGGRPLDYVKQAAKFLVEQVGANDFLSLTVFDSQVDVVFPAQRVTNNDVLKQAVD
                     SIVPGGSTNLSGGLLRGYEETLKECRSDQVNRVLLLTDGMANAGTVDPDMLAGKSGAM
                     LRKGVSLSTVGVGLHFNEDLLIQLAEAGKGSYYYVREPDEIPSVFASELQGLLSVVAQ
                     GISLEVHGLSGCRATAVLGYEPAFDDTGMSLSLPDMFHDEQKLLAVEIDHPALPAGEH
                     EIIRMKLSYADAAHDLDSVSLEVTAKLAVCSAGDQPKEPIFEVMKIVELARTAIAKDR
                     SVEAIDRGDFESGR"
BASE COUNT         2556 a         3514 c         3735 g         2539 t
ORIGIN      
        1 ccctcggcga acgtaggaag cgttggcact ttggcgtagt ttgcgtatgt cgtgagccat
       61 gcttctttgg agaccccggt agtgaccgcg atgatcacca gggtagccaa cgcagcctca
      121 agcaacatgc cgccataccc tactgccagt gcgctgggct ccttatcgat ctgcttcgag
      181 ctggtgccgc tcgatactaa gctgtgaaag cccgatatcg cgccgcacgc aatagtgata
      241 aacaggaacg ggaacatgga aggggccccg gcgggcgacg atactactgc cggtgcaatt
      301 atgggaagtg gccggacgat tatcccaact atcaagagta ccatcgccac gcccagctcc
      361 agcgcgttga tgtagtcgcg cggctgcaac agacgttgga cgggcatggt ggatgcgaag
      421 aagatgtaga tcatgatgat gattatccat gtagccttgc tgagggcgac cgggatgtac
      481 aggcctatcc agatggtgag gtacatcaga actattgcga cgattgatcc gattagcaga
      541 ctccccttct tctcgtacag ataccgcgac aaccccatgg ctatcgcgat ctcaatccag
      601 atcgggaaga cgcattgcgg atattgcaca aacaggttgg ccatgaggat cgcgaatatg
      661 gcgatcacga tccacaagca gaagaagatg atcaggaaga agagcgacct tgtgcgggga
      721 ttcacgactg accccgtgat gtcgccgatg gacctaccgt cgtggcgcat cgacaggacc
      781 agagctccga agtcatgcac ggcgcctgcg aaaatcgatc cgaagaccac ccagagtagg
      841 gcaggtaccc atccccatat cacccctagc acagggccga cgatgggacc ggtgccggcg
      901 atagatgtaa agtggtgacc ggcaaggatg aacttgttgg taggaacgta gtccacaccg
      961 tcgttgtgtt tctcagcagg tgtgatgttc ctggtcgcca gttcgaatac ttttgagccc
     1021 agcttctttc catagaggtt gtacgccagg atgtacaggg cgaacgtcac aaccatcacc
     1081 aagagagaat tcacttagtt cacctccgaa tcgcatttgg gaaaccgcgg atggtcgatg
     1141 cttcctggtc tatcccaact gctgattcgg tacaaattta cggactcctg ctaatttgca
     1201 cctgttatgt atagtcatct ggaactggcg acaaatcata cacttagggc atatcagcag
     1261 gtaagcgatg acactacgaa atctcggaat gcctgagctg caggtgagag cacacgtttc
     1321 gcgtgggtga tcaggtagaa acaccgaacg aggcttaagc cctcaatgtg cagaatgcgg
     1381 atggactgag cggtagtgtc tccttgcatc gcgagactgg ataccacggc tacgccgccg
     1441 ccttcggcga ctgcgctctt tatggcttcg accgagtcga gctgcgcccg gacggagatg
     1501 cgttcgggat ctacgccaag aagcctcaat gcatcgtcga aggtcttttg cgttcctgag
     1561 ccgggctcac ggcacagcat tggtgctcga aggaccgact ccaagggcac tgggtcttcc
     1621 caatgcgagt attcctcggt gaacggggcg atcagcacga gctcatcgcg ggccacggcg
     1681 agatggtcca atgatgagtc ttcgcgagcg cttcctgcca ccccgagctc tgcttcgtag
     1741 ttcctaatcg cgtcccacac ggtagccgaa tcggctcgcg atagctcaac gaagacccca
     1801 gggtaggcat ggaggaagcg cgatactacc cctgggagaa tgtagtcggc aggcacagtg
     1861 ctggcgatca cgcggagcct accggaaacg tcatgcttgt accggcccat tgcggcgacg
     1921 gcctgatctt tgagcatcag aatatcctcg gcgtagcgct tcaggatctt gccggccggt
     1981 gtcagcgcga ttccgcggct ctgccgttcg aacaatgcca cacctagttc cttctcgagg
     2041 gcagccatct gcacgctcac cgtcggctgg gatatgtaga gagccttccc agccgcggtg
     2101 aacgtgccgt ggtttgcgat gcttacaaat atggcaagct gtcgtatgtc cacttcaggc
     2161 cgccccctta aggagccagg atcacatgtt cacgatcagg ctgaagtcgc cggacttggc
     2221 ggcctcgagg attggtccca tgtacttgtc tacttgcgat gccacaagag gcaccggcat
     2281 gttctgcagc ttcgactcca gttgcgggtt cttggcagcg ccgagtgcac gcgagatgtg
     2341 ctcatcagtg agaccaggga gttctgagag cctggtcggg aagccaatct tgcggctcag
     2401 gttggtcatg ccctctgcga ccgctatccc cagttcacgt cctgacagcg cgtcgaggtc
     2461 ttgggcaatg tacccgtagc gccggaatat gtcgcccaca agacggagct tgccctcgat
     2521 cgcaggcgcg aagaatacgg cgtagtaggg gttcattact gcacaggcgc ggccgtgcga
     2581 cgtcacgtcc acaagcgaga agctgtttag atggggcccg ttggtccctc cgaccatgat
     2641 ggcgtaagcc ccgagatcag tggcaaggcc caacgcctct cggggctcgt gcgcatcggg
     2701 atcggccatg accgcaggaa tgctggacac aagcagctcg attccgacct ccgctatctc
     2761 ctgtagcagg ccgaggtttg cgccctttgc gccgaagaac acctccaggc agtgggcgag
     2821 cccatcgaat gccccgtcta gcgtcacatc aggcggcgcg cttcgggtca ccgagtagtc
     2881 gaatacggcc cggtcagggg ttatgatctc atccactatc agctttttct gcccggccca
     2941 ggcatcagtg acattagagt actttgtgag gtgtgctcct gatccggacg cagtctgcac
     3001 agccaccatc gggagcagct tggcgccggt gcgcgtaagc gccgggccca ccagacccat
     3061 tccgtagtag ccttccagct cggggtcgtc atgaccgagg caggcaacta cattggcagc
     3121 cttggctgcg tctatggtgc tgcctccgcc cacggctact atgcagtcag gcttgaagag
     3181 cactatatag agcgccattc tgtacacatc gtccaccagt gaattgggcc tcgatccagg
     3241 ggcaatcctg ccgcccgcca gctctatgcc tgactggccg agggatgaaa cgacctggct
     3301 cgcgatgggc tcaagccatg cgctgcggtt ggctattacc agcgcgcggc gcccgcccag
     3361 ttcgtcggca taggcgccca ctcggccgag tgccccatcc ccgtaggcat atttgtctcc
     3421 tcggaaacct ttgatcaagg cgcgggctcg attcttcaac tcttcagcac gcactctaca
     3481 caaccctttc gtttatctat gtgtccgtat gtcgtgacca tgctcacatt tcggcaagac
     3541 ggtccgaatt ccttgaaagg tttttgctcg gtctgtagaa tctccaatag ggtagaacag
     3601 actcatcttg gggaggtcgg attaccagca gtgttagagc aagtaaaatg cttaattctc
     3661 gatctagacg gtacagtgta cctaggcgac cagttgatac ctggcgctct gctcctgcgc
     3721 caggcggcac aggatttcgg aatcgatgtg ttcttcctca cgaacaattc ctcgcgctct
     3781 cgaagctact acgcgtctaa gcttaccagg atgggatggc cggtaaccgc cgacggcgtg
     3841 ttaagctcat cgcaggccac agcaattgtc ctcaagaacc gcggctatca gcgactctgc
     3901 gtggtgggca ccgatagcct cacggaagag ttgaccgctt atggtctcac ggtgtgcggc
     3961 gatgaagagg aacatgtgga ctgcgtggtc gtggggttcg acacttcgct tgtgtacggc
     4021 aaactgcggg cggccggaag gcacctgcag aggggcacgc ccatgatatc gactaatccg
     4081 gatctggttt gccccttgga aggtggtgag ttcatcccgg actgtgctgc gataagcgcc
     4141 tgcctgatga ctgcgtacgg cgtcgccact gagtacattg gcaagcccga gcgcggaatg
     4201 ttggaccttg tggaggcgaa tacgccatac cgaggcaagc agattgccat gatcggtgac
     4261 aggctgtaca ccgacgtccg ttttggctcc agatggggcc ttttgtcggt gctgacgctg
     4321 tcgggtgagt caagcctgtc ggacttaggc ccggacgacc atcccgactt gatcgtggac
     4381 gatgtagggc agttggccaa gatgatccgt caggcgcgct cgtaggccca tagttccttc
     4441 ttgctggtag atactccaag cgctccggag gccatgatcg attcgatctc gtccaccgtt
     4501 tccaccaggc ccccggctat gaacggcggg accttgtcca tgggcagccg gtgcgccata
     4561 ctgggaacga ccagagctgg aaggatctct actgcatcag ggcgcgatga tgcgatgatc
     4621 tcgaggccgg tcttgagcga ttccgaatcc agcatgaaca ggcgctgaat ggccatgagg
     4681 ccttcgttct tggccgctcg tatcagagtg cttctggtgg tgagtattcc gcgcacgcct
     4741 atctcagttg ccaaccactt gatgcctgcg ggatcctttc cgatgccctg caccaggtcg
     4801 aggtggcaga agacctgcat tcccaggcag tcgccgcgcg cacatacatc gcgcaggtca
     4861 tagagagtgc cggtaaggaa gaacactagc ttagcccggt agcgatgagc agcatctacg
     4921 tcttccacgc gcttgagggc gggtattgcc ggatgcttcg caagatgctg ccatatgcgt
     4981 tcgcggtctc gctggctgag gccgggggtc cattccatgg ccatccactc cttcttagct
     5041 tggaggtacg tacagtgcgc aggttacaat caatgatact tgttacgtct gtggttatcg
     5101 cggcaacctt gggcgctgtc acctatgctg cccctgacgt ccctaactgt ccgctgcatg
     5161 ttgacggcgg cttactgacc attgtgtatg attcgccaga ctcagcgccg gccctttctg
     5221 ttgaatacga cggacttcag ttgctgctgg tccatgcagt tgagccgttc gggcctttcg
     5281 agcagtttgg ctcttcgaga cggctcgaac tgttcgccgg agcgggcttg ccgaagtggg
     5341 gacccggccg tacgggatcg ctgatgatgt cggggcggac agctcccctg ccgcatgtcg
     5401 gttaccacct tgcggtgggc cgcgcagatt atgtccgctt cgtgggcatg atggagtcgg
     5461 agcaacgccg acggctgctc gggcaccggc tggagtattc tccgaccccg tggctgcacc
     5521 tcggggtgag cgagactgct gtggtgtcgg gggaggcctc tcccttgctc tactggccct
     5581 ttcccgggct tccgctgtat gctctgcaac gggctgtcag ccagcgcgac attgcgcagg
     5641 acagtctcat caatgtcaac ttcggagccg acttcgtggc gacactgcct ggggaggggc
     5701 gacggccgga actgcaagca tacggagagc tgctgatcga cgacgcgcag ggaacaatgt
     5761 ccgacaggga ttcggttccg gacttcctgg gcgggctggt cggagtgcgc gttccacgat
     5821 tcacatcgga tcctcgcttc tcggcgtacc ttgagtatgt ggcgatctcg agctacgtat
     5881 actcccacag aaatcccgac aacaactacg tgtaccgcgg aacgataatc ggacatagcc
     5941 tggggcctga cgccgacatg ctggcggttg tcacggattt ccgtccggac tccaaaacag
     6001 cggtgtcgct taccggcgcc gttgttcgcc atggtgagag cgagatcggc catccgtggt
     6061 cgcctgcgga gggccgcgat cgcgagttcc cgagcggcat cgttgaaaag acgctcaagg
     6121 catcgctgga agtggagagg atgctgcttg agcccgtgtt gatcgttggg cgattggagc
     6181 ttggtacaac gttcaacaag gggcacgtcc agggcagtac acagaggtac tggagtgccg
     6241 cggcaggtat cggcgtcagg ctgtagcggg cagcgacggt gcgcaggaat cagaacacga
     6301 accgcagccc tgacctgagc ttgccgtagt tgcgcggcgg gatgagccat tcgaactcgg
     6361 cgaaccatgc gccgaactcg cctccgccga ccaggtgtag cccggttgag tcaggggcga
     6421 actcacgggt gaggcccacc cctccgtaga ggcaggtcaa gatcgtgaac tgcctcgggt
     6481 agactaggag caccgacgcc ccggcagacc ccggcccgcg cctgatgtca aggttgaccg
     6541 cgagacgcag gtattgtgag agacttgctc ccaggcctgc gctgacaccg tctgcgagga
     6601 tgaaagacac ctgtgcacga tccagatggg gccatgcaga gcctgctgcc gaggcctgcg
     6661 ccggcagcat tgccagtgcg actgtgcata gcagaaccag tgtgacgagt gcggtagtct
     6721 tcttcattga atacgctcct tatgtacata cattctgcgt ggaggacccg atattgagag
     6781 acgcaaatgg acccaaagcc gataaatact ccgagtcatg ggccgccctg tggtggctct
     6841 tagcactggc atacccagtt ctcctgatag tcgccgcaag tcgccccgaa ttcggagccg
     6901 agttcaccca gatgctggtt aaacgactgt tgccgggcct tgcaggccgg caggttcacg
     6961 tgctggtggt ggcaatacgc aagtccggac atttcctcgg ctattcttta ttcgccacga
     7021 ttctcgtaaa cgcctttctg tctttctcac gccctccgcg caaccgcagg accgtggtgg
     7081 tcgcgtacct ggcggccgca atcgcggccg tgggggtcgc ggccatagac gagtacatcc
     7141 aggtgagcgc ggcattcaga tccgggtcgc gacaggacgt gctgctggac tcactgggtg
     7201 tgctagcggc tttggtcgtc cggatcaggc gccccgagta gggcacgcgg tcagcaccca
     7261 tgtgggaata cacacatgtg ggaacacgtg tcgcttccgg cacgtatgag ctataatagg
     7321 aaaggctgtc tgtcgaactc gcgtcggcgg cctgaaggat ggtggaggat tgatgttgat
     7381 gactcttcga aaggctaagt tcgctgcgtt aacgatacta gtgggagttt tcgccctttt
     7441 agcagcgtgc ccgggttcgg ctctagcggc gcaccttatc gtgaccctaa ccgacggctc
     7501 acaagttacg tacaacattc cggttgacga gtcgaatgtt cagtcagtgg ctctgggctc
     7561 atcgcagcaa tcaatgaccg atgccgaagc ggccattgcc acggtgaacg gccaacccat
     7621 tactaggcag gatttttaca tagctctatc ggaggcagcg gggaggcctg tgctggatca
     7681 gctcataagc gaaatgctgc ttgcacaggc tgcgaccgag gctggtgttt cggtgtccaa
     7741 tgaagaagtg gatcgggaat tcgaatccgt tcgccagcag gtgggcccgg aatttgagag
     7801 tcttttggcg cagtatggaa tgaccgccga cgacctcagg aagaacatca aaatcagcct
     7861 gctcgtcttc agagtgtcta cgaaggacgt tgtggtgaca gatgagcaga ttgctgccta
     7921 ctacaacgaa cacacggccg acttcgagag ccctgagatg gtcaaggcca gtcacatact
     7981 ggtcgaaacc gaagcagacg cgcaggcggc tctggaccga atcagcgcag gagcggattt
     8041 tgccgacgtg gcggcagctg tgtcgatgga tcctatgacg gcccagaacg ggggagacct
     8101 cgggttcttt gccgcgggag agatggtgcc cgagtttggg gacgctgcgt ttgcattgaa
     8161 gactggtgag atcagcggga tagtaaagac tgactatggt taccatatca tccaggttac
     8221 tgacagggtt gagccccgcc gcgcgtcctt tgaggaggct agcggcgtaa tcgagcgact
     8281 gcttagaagc gcccatgcga tgaaaccggc ggatctcatc gctcagctta aggagacgag
     8341 tctgatcact gtgttcgatt ccaggttcac ggatctgggt acaataccgc taccttagga
     8401 gttgtggtct tatgctgcgg acggttctat tcgacctcga cggcacgttg gtacatttcg
     8461 agttcgatga tttcatgaag gcgtacctag gggccatcag caacaaggtt gcgcagctag
     8521 ttgacgccaa ggtgtttgtg gcccagctac ttgcgtctac agaggagatg gtcaggtcac
     8581 tcgatcccgc gaggaccaac cacgacgtgt ttgctgacca tttcttctca ggtctggggc
     8641 gcacggaaga tgagctgatg cctgtattcg acgcattcta cgatgaagac tttccgcgtc
     8701 ttcgtgacgc gcttggcgta gagccgcacc ccctggcgcg gccgatgatc caagggctga
     8761 tcgatcgcgg ttacgaagtg gtgatcgcca ccaatcctgt ctttccggcc aatgccatag
     8821 aggagcggat gagatggggc ggactggatg gtatacctta ccgacacgtt acctcctacg
     8881 agacttcgca tttttgcaaa cccaacactg agtactacgc ggagatcctc gatgtagtgg
     8941 gcagatcgcc tcacgagtgc atgatggttg gcaacgacac gtgcgaggac ctggtggcag
     9001 gggaacttgg tatcactacc tacctggtcg aagattacat gctcgaccga ggcccctacc
     9061 gggcagttcc ggatcatcgg ggtcgcttcg aggacctggc gcggttcctg ggatctctgg
     9121 gttgcgttgg cgcctgacag gcgccccatc gacaagcacc gcatcgccca tcgcccgacg
     9181 gttactccga tccgaccgaa gcgccgccgg agtccgccgg gctggctggc gcggccggac
     9241 ttgccgggtt ggccgggtta gccaggtgga ttatgccggc tacctgtgac acgtcgagca
     9301 cgaacgctat gccagttccg ggccgctcaa gctggccggc ctttatgatc gcttgcagca
     9361 ctgactgcgt gcgctctcgg ggaatgaggg tgaggactac ctctttctcc ggctcaatca
     9421 gtattccgaa gaagctcttg cgctcatgca ctccggtgcc ccgcccgtag agcacggtgc
     9481 cgccctcggc gcccgcgctc cgcgcagctt ccactatctc gtctgcccat cccttgttta
     9541 tgatcgttac gatgagatcg aactccccac cgacggttgg tctttcgtgt gacatatcga
     9601 attcccccca tcagaagtat ctgcctcgcc gggcacggcg atgtctttct tcgctctgaa
     9661 tgcgaaactc aacccctcca ctgcaagcgt ggaagccagc gcaatgaggc acacaagccc
     9721 gaaacccgag ccgatggtcg gccctcccgg ctgctgggcc gcgatgccca ggcccagcga
     9781 catgaggaag gttaccgtaa gcgggccggt cgccaccgcg cctgagtcga aagatatcgc
     9841 ggcgaacgcc ggatcgatcc atcggagaag cgcgagggcg accccgtagc ctgggatgat
     9901 gatgtgggtg agcggacgac cacgtgcgac gaaatagtag cccagcgata ccagtgacgc
     9961 tactcctaga gccatggtgc cgagtaacaa tcgttcgcgg atgtggcctg ccgaggcctt
    10021 ttccacttcg ccgctgagca cgcgggcgga cggttcggcc aacaccacga gcagaccgag
    10081 cacgaacccg acgggcagaa ccgcgatcgg gttgtccatc ttgccgagct gagcgccgag
    10141 cgcccgaccg aggggcacaa aggcaatgtt gacgccctga aggaagaacg agaggcctat
    10201 gaatgtccac acaatgccta ccgccacacg acgcacgaaa gtgcgcggca gcttgaggta
    10261 gaatacctgg aagactgtga acagcgcgcc cagcggtatc agtgtgcgcg ccacttcggc
    10321 catcacttgc ccaaacccgt gaaacaccac tatgcgatcc atcacatgaa cactccccac
    10381 accattagca ccagtatagg gccgagcgtc gccagtccta ccagcccgaa ctcgtcggcc
    10441 agggtctgtt ttcgttgaag cacagacgct gtgcccagac caagggcgat gatgcacggc
    10501 acggccatgg gccctgtaga cacgctgccc gagtcgagga agagcggcac tagctgaggg
    10561 tgtgtgaatg ccgaaagcac aattaccgcc gcgtaggccc cggcgaacag ccatttgata
    10621 gggatgttga gaagtatcct gagtattgcg atggtggcca aaagccccat cccgccggat
    10681 agcagaaccg ccaggacctg cgggttgatg cccgaatcgg gaaacgagcc gacctgcgcg
    10741 accaaaacgt gcactgacgg atcagcgatc gtgacgctga aagtgaagat gaaggctgtg
    10801 gccaccagta gcgcggtcga cccgcgcctg ggcaagtagg cgccgatcat ctcacccatt
    10861 ggaagtagcg ctatgcgggt gcctctgaga aacaggaaca gacctgctgt ggttagcacg
    10921 gccccgacca ggagttgtgc cagcatcacc catgacaggc ccaggaagac cgtactcagg
    10981 atcgctacgg ccaaggtgag cggcgctact gcgttgacta cttctgaaac ggctgcacga
    11041 aattggcgag acaccgagca ctctcctttc tgattgtatc ttcgcttcgc gtcgcgagag
    11101 tcctcttagt cgtcgccttc gatatcctgg agatatgtga gattgagacg caccaggaaa
    11161 tcctcttgca gttgatgaag gtgaaatcgg ctgccgtgac tatgaagtaa agtgaaggta
    11221 cccatatcgt ttagaacgga gaggctgaag ttgggctgta atcatactgg tgaccacgga
    11281 aattcggtgc aggcgcaggt catgttgtct cattcagtat tgccggtggg caggcccgga
    11341 aagctgtggg cagtcgtaag gctgtcgagc gggtcggtac aacaaggcgc tgctgaacgg
    11401 ctgccgctca atgtgggcgt ggttctcgac cgaagcggtt ccatgggggg gcgccctctc
    11461 gactatgtca aacaggccgc caagttcctg gtggaacagg ttggcgcgaa cgacttcctc
    11521 tcactgacgg tgttcgacag ccaggtcgat gtggtcttcc cggcgcagcg cgtaaccaat
    11581 aatgacgtgc ttaagcaggc cgttgattcg attgttcccg gaggatcaac taacctatcg
    11641 ggcgggctgc tcagaggcta cgaggagacc ctgaaagagt gcaggtcgga tcaggtcaac
    11701 cgagtcctcc tgctcactga tggaatggcc aacgccggca cagtagaccc cgatatgctg
    11761 gccggcaagt ctggggcaat gctgcgtaag ggagtgtcgc tctccactgt cggagtagga
    11821 ctccacttca acgaagacct gctgattcag ctggcggaag caggaaaggg gtcctactac
    11881 tacgttcggg agcctgacga aatccccagc gtattcgcgt ccgagcttca ggggctgctc
    11941 tcggtggtcg cacaaggtat cagcctggaa gtgcacggcc tatcgggatg tcgcgcaaca
    12001 gcagtcctcg gttatgagcc ggcgtttgac gacaccggca tgtcgctcag tctgcccgac
    12061 atgttccacg atgagcagaa gttgctggcg gtggaaatcg accatcccgc tctgcctgca
    12121 ggcgaacacg agatcatcag gatgaaactg tcatatgcag acgccgccca cgacttggat
    12181 tcggtgtcgc tcgaggtaac ggccaagcta gctgtgtgct ccgcaggcga ccagccaaag
    12241 gagcctatct tcgaggtcat gaagatcgtt gagcttgcca ggaccgcgat tgcaaaagac
    12301 cggagcgtcg aagcaataga tcgcggcgac ttcgaatctg gccg
//
LOCUS       DAOTAC010000035        12326 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_4348_length_12326_cov_5.161275, whole genome shotgun sequence.
ACCESSION   DAOTAC010000035 DAOTAC010000000
VERSION     DAOTAC010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 12326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12326
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4348_length_12326_cov_5.161275"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..152
                     /locus_tag="PLM74_02645"
     CDS             <1..152
                     /locus_tag="PLM74_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein"
                     /protein_id="HON42017.1"
                     /translation="VPSPLAPPPGCPFHPRCPRAKPDCMEPGRPPREVEVAPGHRVAC
                     NYPVE"
     gene            276..851
                     /locus_tag="PLM74_02650"
     CDS             276..851
                     /locus_tag="PLM74_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42018.1"
                     /translation="MESEPNVYAEGAITWAVDKLGITDYAFLCYLFVEDAYELGNSIV
                     LDGQGSTAKEAADAYCAREHRGVPPRGAYVFYDCLGTIEGEYRNWGHIGLSLGDGQVI
                     HAWNRARTDHYLGIEELTPGPGFEKPQYVGWTPVATILRGMTVTRGTSGHQARLVIAA
                     VRSFSTRGSSLEGSTACASYHRGSHCKSRPC"
     gene            787..1551
                     /locus_tag="PLM74_02655"
     CDS             787..1551
                     /locus_tag="PLM74_02655"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HON42019.1"
                     /translation="MKAALLVPRITADRTANLDRVEQMAADAAGSGAGLVLLPEAVLT
                     GLINNDDRAHDLPLGQSVPGPATERLGAVACLNGLWIGFGLIERDGDCLYDSAVLIAP
                     DGSVALRYRRNQPHWHGVAADPAIYRHGTSIEVARTPIGNVGFLICGDLFDDALVVRF
                     RALRPDLLLFPFARCFPDGSADQARWDAEEMPEYARQVASVGAPALMTNYIGDPDIEE
                     CPSFGGAFAVSAGGEVVASLPLGVEGILLVDLDRLL"
     gene            1624..2097
                     /locus_tag="PLM74_02660"
     CDS             1624..2097
                     /locus_tag="PLM74_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008594583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HON42020.1"
                     /translation="MHDPDVHFKRITAATVLDVCKLSQTLSDEQRKMVADNAVSLAQA
                     AYSDGAWVRAIYADSDLVGFIMLHLGSDYDDGIDCPGVFLWRLMIAGPFQGKGYGARA
                     IHRMLAHLRSMGVRELYTCCETGPAGPEGFYRRLGFEPTGDCYGDQPEYVLRLDE"
     gene            2094..2249
                     /locus_tag="PLM74_02665"
     CDS             2094..2249
                     /locus_tag="PLM74_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42021.1"
                     /translation="MSSEDDKPFAAPNKRAPWDELGLADQLELLSAAADTRIRQTGLS
                     ADALSVW"
     gene            2534..2749
                     /locus_tag="PLM74_02670"
     CDS             2534..2749
                     /locus_tag="PLM74_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004163690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HON42022.1"
                     /translation="MTNEFTAVIEQDCEWFIAYSPEVPGANGQGRTKDEALKSLSEAI
                     RLILEDRREQGLRGIPPDAVVERVSVQ"
     gene            2890..3522
                     /locus_tag="PLM74_02675"
     CDS             2890..3522
                     /locus_tag="PLM74_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42023.1"
                     /translation="MRAWNRAVSMIVLGSIAPIALMLLGWWGTLWLLGDTPWIPWMAG
                     AGFLAGLTLDATILRSRLGSLYTIPWPALVCVAVFYSVLIYGFFMAFPVPNLIVGILG
                     GFVVGSRHGGDRIRTLSAEHDARIAAVVSAGILFVLCCVTAWLALTEPTITSQVQHML
                     GLPFEPTIGLLRVVTLIGGLGLVAVEYFATLATADWARRAGPAGTAEDRR"
     gene            3738..5624
                     /locus_tag="PLM74_02680"
     CDS             3738..5624
                     /locus_tag="PLM74_02680"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HON42024.1"
                     /translation="MRYRKPLLALIICLGLTSLAGCNGCDGVGTKTFSITGRVTTAGL
                     GHPVTAHPVTGAAVSVGQVAATTNSAGAFELHGLTTTDSQVTITVTKKDYAPATATVA
                     VFNNATIVQDFYLVSAPAGTGIVSVDGRVEFMASELYGRVAAASHDAWTDPVALGEPD
                     SVLVELESDLTAANVDALLRDVGSSAYEIRQIINRAIVKVPQGRSLDEFMDEIKRSPM
                     VRSVEPNGLVAAARVPNDPSYSQQAYWYGAMNLPASWETITGRADITIAVVDTGLRYN
                     HPDIGNLVPGWDFVDGDSDPWDPGKPGQSISSHGTHVAGTIGALTNNSIGVAGINWNA
                     RIMSVRVLDTTGRGSWADISSGIRWAANAGADVINLSLGGYEGSSVLQEAVSYAARRG
                     AIIVAASGNDGISSVMYPAAYPEVIAVGAAAKPNSVADFSNGGTSLDLIAPGVSVYST
                     NYSASTGHGYAYAQGTSMASPHVAGLVGLMLANGVPSSQVREVLRETAIRPQDMLGWD
                     TWFGYGFVNAYAAVTNARLTRAALVATNDQFDAISSVVYPGSNRQFSMTRLPQAAALY
                     VFGWIDVNNSRSLDDGDYTGYAQLSTLGKTQVPAFITLNIHSEWSPSIQETINKGLGA
                     IGVR"
     gene            6180..6893
                     /locus_tag="PLM74_02685"
     CDS             6180..6893
                     /locus_tag="PLM74_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015052651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HON42025.1"
                     /translation="MKVTVFNGSPRGRNSNTHRIVEPLLEGAREAGAETEEVFLVERD
                     IKHCRGCFGCWGKTPGKCVINDEMPRLMDLFLESDYVGLGTPIYSMYMTGLLKSFTER
                     LLPLATPHIRKNADGTFYHDGRVKRFPRVFCVANCGFPGEHNFDLLKAMVVQMSPVLE
                     VYRNCGAILSDAGAADIPAISEFYDALRQAGKEMVTGGRVSDETVRKIHAELISDEEY
                     MAHANKDWDEEIRKAESQG"
     gene            7013..8566
                     /locus_tag="PLM74_02690"
     CDS             7013..8566
                     /locus_tag="PLM74_02690"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HON42026.1"
                     /translation="MRRWAKQRWLAAVILVGALGAMGALGAVATTTAVAEDAAAGFPD
                     MSAIDRLVDRQMNKHRIPGISLVVFEDGEVTYAKGYGTAGHGQPMTPDTPMIIGSTTK
                     PFTAVSILQLVEQDLVDLDSPVKAYLPWFRVSDPVASTSITIRHLLNHTSGLSELGYN
                     RVLRSDATLEQGVRDLQHARLTAPVGTTFQYFNPNYATLALIIETVSGQSYANYATEH
                     IFETLGMTNSYADKEDALEAGLAQGHSKLFGFPVARGPVFRAYLLGAGYIVSTARDLS
                     RFLMAIDNEGAYEGGRILSSDSVALMASPPDQVRGTSYGMGWSAGTHRGEPIGGHGGS
                     DEVYSCQTALLTDRHRGYALLMNEQHLIDGMVASSQLQIGLRDLLIGRQPDDGGISMK
                     LIGIVILAVFLIALVGTIRSFIRMRGWSREARSLTARQIAWRIAPHLLVPALTLIVLY
                     SFLGSVILREPRAWNFRVVGAYFMPDIALLLLLAIVPDIVQGIYMIAAAMADRPAGRA
                     ARPRGSSRR"
     gene            complement(8667..9125)
                     /locus_tag="PLM74_02695"
     CDS             complement(8667..9125)
                     /locus_tag="PLM74_02695"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004815022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HON42027.1"
                     /translation="MRVLVLNRPNLNMLGIRERQLYGDRTYSELVEFIKQEGKQKGVF
                     VQVSQSNCEGRLITAIQRARGRFDAIILNAAGYTFTNIAILDALKAVSLPVVEVHLTD
                     IGSREDFRKFSYVALYAEKVIVGKGFEGYAEALDYLFDKYGSSGGDGGKG"
     gene            10083..10328
                     /locus_tag="PLM74_02700"
     CDS             10083..10328
                     /locus_tag="PLM74_02700"
                     /inference="COORDINATES: protein motif:HMM:NF025210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HON42028.1"
                     /translation="MCLQELIPDQVLYEGDWRVLKVLGPLSFSLTGILAAISSVLAEA
                     GVSIFSVSTYNTDYILVKYDRLEDAMEALTGRGVEFI"
     gene            10441..11514
                     /locus_tag="PLM74_02705"
     CDS             10441..11514
                     /locus_tag="PLM74_02705"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HON42029.1"
                     /translation="MRGTARRRPRIANVVLIGLATVVVLFSVCSMVIVKTMYDKQFQR
                     ADKPRFSGYLTYGDVDGYHRRAVRFKSGNNALTGYVYGERNDKGLVVIAHGLGGGAES
                     YLAETMYFVDNGWRVFSFDCTGSHESEGKGTIGLPQSVLDLNSALSYIESDSSLDGLP
                     AMLYGHSWGGYAVAAVLNYGHDIAAAASIAGFNAPMEILFERAEKMMGSIAYLEYPVL
                     WAYQTMLFGRAARLTAVDGINSADTAVMIIHGNKDTTVSYNRTGVIAHQGEITNPNVV
                     YKTCTAENHNGHRNLFRSEAAVEYTNQKNRVYQELHDLYNGSIPDDVLADYYANIDKT
                     LTSELDTGFMDEINRFFERCLPD"
BASE COUNT         2602 a         3362 c         3730 g         2632 t
ORIGIN      
        1 atgtgccgag cccgcttgct ccgcccccgg gctgtccatt ccatccgcgc tgccccaggg
       61 ccaagcccga ctgcatggaa cccggccgcc caccgcgcga agtggaagtg gcgccagggc
      121 acagagttgc gtgcaattac ccggtggagt gagcggggtt agctcgacca caggacagac
      181 gtcgatggac ttgtcgactc gcccgcttgt tagccgggtc ataccattca tgatactgtc
      241 acggcatgag ttgggatgag aagaggggga ctctattgga aagtgaaccc aatgtgtatg
      301 ccgagggcgc catcacctgg gcagttgata agctgggtat caccgattat gcgtttctgt
      361 gctatctgtt cgttgaggat gcttacgagc ttggaaacag catcgtgctt gacggacagg
      421 ggtcgacagc caaggaggca gcagacgcgt actgtgcgcg agagcatcga ggcgttccac
      481 cgagaggcgc atacgtcttc tacgattgct taggaaccat cgagggcgag taccgtaact
      541 ggggccatat tggattgtct cttggtgatg ggcaagtcat ccatgcatgg aaccgagccc
      601 gcaccgacca ctacttaggt atcgaagagt tgactcccgg acctgggttt gagaagccgc
      661 agtacgttgg atggactccg gtggcgacga tattgcgcgg gatgaccgta actcgaggga
      721 cctcgggcca ccaagcgcgt ctggtgatcg ccgctgtccg ctccttcagc actaggggga
      781 gtagccttga aggcagcact gcttgtgcct cgtatcaccg cggatcgcac tgcaaatctc
      841 gaccgtgttg agcagatggc ggcggacgct gccggctcgg gtgccgggct cgtactgctg
      901 ccggaagctg tgctcacggg cctgatcaac aacgatgacc gggcgcacga tcttccgctc
      961 ggtcagtccg ttccgggccc ggcaacggag agactcgggg ctgtagcttg tctgaacggc
     1021 ctgtggattg gttttggact gattgagaga gacggcgatt gtctatatga ctccgccgtc
     1081 ctgatcgcac cggacggctc cgttgccttg aggtatagac ggaaccaacc acactggcac
     1141 ggtgtcgctg cggacccagc catctacagg cacggaacct ccatcgaagt cgcccgcacg
     1201 cccatcggga atgtcggctt tctcatctgc ggcgacctct tcgatgatgc cctggttgtc
     1261 cgcttcagag cactccgccc tgacctgctc ctgttccctt tcgcccggtg ttttccggat
     1321 gggtcggcag atcaggctcg ctgggatgca gaggagatgc cggagtatgc tcgccaggtc
     1381 gcgtcggtgg gcgcgcccgc tctcatgacc aactacatcg gcgatcccga tattgaggag
     1441 tgcccgagct tcggaggcgc cttcgccgtt tcggcaggcg gtgaggttgt agcgtccctg
     1501 ccgctgggtg tggaaggtat tcttcttgtc gacctagata gacttctttg agacgattgt
     1561 gtgcgggcgt gccggttttg ctgctgcgct tgactgaaga tcagcggagg aggagatagg
     1621 catatgcatg atcctgatgt acacttcaag cgaataactg ctgccacagt gctggacgta
     1681 tgcaagctca gtcaaaccct ctctgatgag cagcgtaaga tggttgccga caacgcagtc
     1741 tcgctcgcgc aagccgccta ttcagacgga gcgtgggtga gagcaatcta cgcggacagc
     1801 gatctcgtcg gcttcataat gctgcatctc ggatcggact acgacgatgg gattgactgc
     1861 cctggcgtgt tcctctggag actgatgatt gcagggcctt ttcaggggaa aggatacggc
     1921 gctcgcgcca tacataggat gcttgctcat ctgcggtcga tgggtgtgcg cgaactatac
     1981 acgtgctgcg agactggtcc ggcaggcccg gaggggttct accggcgact tgggttcgag
     2041 cccactggag actgctacgg agaccagccc gaatacgtcc tgaggctgga cgagtgagct
     2101 ccgaagacga caagccgttc gcggcgccta acaagcgtgc cccgtgggac gaacttgggc
     2161 tggcggatca actggaactg ctgagcgccg ccgctgacac gcgaatccgc cagaccgggc
     2221 tgagtgctga cgcactatcg gtgtggtaag atcatcttgg cacttgacgt aacggcaaga
     2281 aggtggtacc agcaatcgcc gtaactgaaa cactgatcag cgagatggtg ctggtagcgg
     2341 catccgaatg cagtcttgac gctgacatcc tctctggatc ctgcgctggc gctgcggacg
     2401 agaattcggg tgtagatctt gtgattgtca gagctaggcc tttctacagc ggaccgagtg
     2461 tacggagaaa ccaagatgtc aagatatgcc gagatgacag acataccggc atgaatagag
     2521 gaggccggag cctatgacca acgagtttac ggcagtcatc gagcaagact gcgagtggtt
     2581 catcgcctac tctcccgaag tgcctggcgc caacggccag gggaggacga aggatgaagc
     2641 gctcaagagc ctttcggagg ctatccgcct gatcctcgag gaccgaaggg agcaggggtt
     2701 gcgcggcatc ccccctgacg cggtcgtcga gagggttagt gtccagtgaa acgaggcgca
     2761 ccgcaagcat gtccagggca cgggatcgag tgcggtggct cagctcgggc ctcagagcgc
     2821 cgccgctgac acaggccacc tcagctggca tcatacctgc gatgattagc gtaatggggg
     2881 tctgcttgca tgcgagcgtg gaatagggct gtgtccatga tcgtgctggg cagtatcgca
     2941 cctattgccc tgatgctcct cggctggtgg gggacgctct ggctccttgg cgataccccg
     3001 tggattccat ggatggcagg ggccggtttc cttgccgggc tcacactgga cgcgacgatc
     3061 ctgcgcagcc gattgggttc gctatacacc attccgtggc cggccctcgt gtgtgtggcg
     3121 gtcttctact cggtcttgat ctacggattc ttcatggcct ttccggttcc aaacctgatt
     3181 gtcgggatac tcggcggttt tgtcgtaggt agtcgccacg gcggagatcg catacgcacc
     3241 ttgagtgccg agcacgacgc tcgcattgca gcggttgttt ctgcaggcat tctgttcgtg
     3301 ctatgttgtg tgaccgcgtg gttagcgctg acagagccta ctattactag tcaggtgcag
     3361 cacatgctcg gcttgccatt cgagcccacc ataggtctgc tgcgagtggt cacgctcatc
     3421 ggaggtctgg gtttagtggc tgtagagtac ttcgccacat tggccactgc ggactgggca
     3481 cgacgtgctg gtccggccgg aaccgcagag gatcgccgct gacatatgcg gctaccagat
     3541 cgccccatcc tcaaacctgc ctaagtcaaa cctcgaagaa ggttgataac gggactggca
     3601 tttctggaag gattacgcag tgttctatcg aaatacaagt ctgcgaggat tccagaatct
     3661 cgggggccga aggactatgg ctcccacttg ctttggtgca ggtaggccgt cccaagactt
     3721 cgacgaggag gaaaaacatg aggtatcgca agccattgct cgcactcatc atttgcttgg
     3781 gcctcacatc gctggcgggt tgtaacggtt gcgatggggt agggactaag acattcagta
     3841 tcactgggcg tgtgacaacc gctggccttg gccacccagt gacagcacat ccagtcacag
     3901 gagcagcagt ttcagtaggc caggttgcgg ccacgacaaa cagcgcaggt gcatttgagc
     3961 tccacgggct tacgacgact gacagtcagg tgacgatcac agtcaccaag aaagactacg
     4021 ccccggcaac ggccaccgtt gcggtcttca acaacgccac catagtgcag gacttctatc
     4081 ttgtatctgc tccggccggt acagggatag tctctgtgga tggccgggtt gagttcatgg
     4141 cgagcgaact gtatgggaga gttgccgctg cctcgcacga tgcgtggacc gatcctgtcg
     4201 ccttggggga gccggatagt gtgctcgtgg aactagagag tgaccttact gccgccaacg
     4261 ttgatgcgct tttacgagat gtcgggagta gtgcgtacga gatcaggcaa atcatcaaca
     4321 gggcaatagt gaaggttcct caagggcgat cactagacga gtttatggac gagatcaaga
     4381 gaagtcccat ggtgagatcc gttgaaccca atggtcttgt agcggctgcc agggtcccca
     4441 atgatccgag ctactcacag caagcctact ggtatggggc catgaatctc cccgcctcat
     4501 gggaaacgat cacaggaaga gcagacatta ctatcgcggt agtcgatact ggtctacggt
     4561 acaatcaccc tgatatcggc aacctcgttc caggctggga cttcgtggac ggagatagtg
     4621 acccgtggga tccgggtaag ccaggccaat caatcagcag tcacggcacc catgtggctg
     4681 gaacgatagg ggcactgaca aacaactcta ttggcgttgc tggtataaac tggaacgcgc
     4741 gaattatgtc agttcgcgtg ctcgacacga cgggtcgggg ctcgtgggca gatatctcct
     4801 caggaatccg gtgggccgcc aatgcagggg cggatgtcat caaccttagt ctaggtggtt
     4861 acgagggctc aagtgtgcta caggaggcgg tctcttacgc tgcacgtaga ggcgccataa
     4921 tcgtcgcggc gtccggcaat gacgggattt cctcagtgat gtatccggcc gcttatccgg
     4981 aagtgatagc cgtcggggcc gccgcgaagc ctaatagcgt agccgacttt tccaacggag
     5041 ggactagcct cgatctcatc gctcctgggg tcagtgtata tagcaccaat tacagtgcct
     5101 cgactgggca tggctatgca tatgcgcagg ggacctcaat ggcgtcgccc cacgttgccg
     5161 ggctggtagg gctgatgctg gcgaacggcg taccatcatc ccaagtgcga gaggtgttac
     5221 gggagacagc gatacgacca caagacatgc ttggatggga cacgtggttc ggttacggtt
     5281 tcgtgaatgc ttacgctgca gttactaatg cccgtctcac gagggctgct ctcgtcgcca
     5341 caaacgacca attcgacgcc atcagctctg tcgtgtaccc agggagtaac aggcagttca
     5401 gcatgactcg cttaccgcaa gctgcggcgc tatatgtctt cgggtggatc gatgtcaaca
     5461 actctcggtc tctggatgac ggcgactaca caggctatgc gcagctttct acgttaggca
     5521 agacgcaggt gcccgcgttc attacgctga acattcattc ggaatggagt ccgagcatcc
     5581 aggagaccat caacaagggt cttggcgcta tcggcgtgag gtgagctgaa gacggcccct
     5641 ataaagagat catggcgcaa ggcaatctcc tgctagttgg ttccatcggt ctacactcct
     5701 gatttgaccg tcggcaggga gtgaaacgaa cgtgctcgcc tccgaacgcg acccagagga
     5761 agctgaaagc gtcagcgaag tgcgtagcgc cgggcaggta tcaagacctg cccggcgcca
     5821 acgtttcacc cgggcgtcgc gtggttacgc ccatctggac atccgaccct gggttctggc
     5881 aatcccgctc gtctctcctc tccaactttg tagcgttcac cgcagatgct gaacgttaca
     5941 tccacacatg ctgccctgag gcgctccttg tcaactatgc caatccaatg tcgatgctgt
     6001 gccgtgccat agaatcggtt ttgtctaaag ggccctgagt atcggcacag gaagaaccag
     6061 agtcttgacg aatcgttccg gagataagtg aggcgcattg ggcgccgggg agtctcaaga
     6121 gaagagcaag acggaaccct agtcgaggac aagacgggga taagagggga gcggtctaga
     6181 tgaaggtgac agtcttcaac ggcagcccgc gggggcgcaa cagcaacact cataggatcg
     6241 ttgaaccgct gctggaaggg gcaagagagg caggagccga aacagaagag gtattccttg
     6301 tagagcgcga cattaagcac tgccggggct gctttggctg ttggggcaag acaccgggaa
     6361 agtgtgtgat caacgatgag atgcccagac tgatggatct ctttcttgag tcggactacg
     6421 tgggactggg cactccgatc tactctatgt acatgacggg attgctgaag agcttcacgg
     6481 aaagactcct gcctctagcc acgcctcaca tccgcaagaa tgcggatggc accttctatc
     6541 atgacggcag agtcaagcga ttcccgcgcg tgttctgcgt ggcgaattgc ggattccccg
     6601 gggagcacaa cttcgacctg cttaaggcca tggttgttca gatgtcccct gtgctggagg
     6661 tctatcgtaa ctgcggagcg attcttagtg acgccggagc agctgacatc ccagcgatta
     6721 gcgagttcta cgacgcactg cggcaggccg gcaaggagat ggttaccggc ggtcgggtct
     6781 ctgacgagac tgtcaggaaa attcacgccg agttgatcag cgatgaggag tatatggctc
     6841 acgctaacaa ggactgggat gaggagatac gcaaggcaga atcacagggc tagcaggtga
     6901 ttgtgccaga gggggtatcc cttcgaagta ttgctttgca gactcacttg tgagatgatg
     6961 gtgctgtttc cagcggaagg atactcgaaa gcgacttggg gagggctgcc aagtgagacg
     7021 ttgggcaaaa cagaggtggc ttgcggctgt tatcctagtt ggcgccctgg gcgctatggg
     7081 cgctctaggc gctgtggcta cgacgacggc tgttgcagag gatgcggcgg caggctttcc
     7141 cgacatgtcg gctatcgatc gcctcgtaga ccggcagatg aacaagcacc gcatcccggg
     7201 catctcgctt gtcgtgttcg aggacggtga ggtgacctat gccaaaggct acggcaccgc
     7261 agggcatggg caacccatga cgcctgatac accgatgatc ataggttcga ccacgaagcc
     7321 gttcacagca gtgtctatct tgcagctagt cgagcaggac ctcgtcgacc ttgactcgcc
     7381 tgtcaaagct tacctgccgt ggttccgcgt gtcagacccc gttgcttcga cgagcatcac
     7441 tatccgccat ctattgaacc atacaagcgg actctccgag ctcggttaca accgcgtgct
     7501 acgtagcgac gcgacgcttg agcaaggggt gcgtgacctc cagcatgccc ggttgactgc
     7561 gcctgtgggc actacattcc agtacttcaa ccccaactac gcgaccctgg cgctgattat
     7621 cgagacagtc agcgggcagt cctacgcgaa ctatgcgact gaacacatct tcgagacgct
     7681 ggggatgacc aacagctatg ccgataaaga ggatgctctg gaggcagggt tggcccaggg
     7741 gcacagcaag ctgtttggtt tccctgtagc ccgcgggcct gtcttccgcg cctacctgct
     7801 gggtgcaggc tacatcgttt cgactgcacg cgacctgagc cgttttctga tggcaataga
     7861 caatgagggg gcctatgaag gcggcaggat cttgtcctcg gatagcgtag cactcatggc
     7921 gtcgccgcct gaccaggtgc ggggcactag ctacgggatg ggctggagcg ccggcacaca
     7981 ccgcggagag ccgatcggcg ggcacggagg ttccgatgag gtgtattcgt gtcagacggc
     8041 gttactaaca gaccggcatc gcggctatgc cttgctcatg aacgaacagc atctcataga
     8101 cggcatggtg gcctccagcc aacttcagat cggcctccgg gacttgctca tcgggcgaca
     8161 gcccgatgat ggtgggattt caatgaagct catcggcatc gtcatacttg ccgtgttcct
     8221 gatcgcgctc gtaggcacga ttcgttcctt catacgcatg cgtggatgga gtcgcgaagc
     8281 caggtctctt acagctcggc agatagcgtg gcggatcgcg ccgcacctgt tggttccggc
     8341 tctcacccta atcgtgctgt acagcttcct cgggagtgtg attctcagag agccgagggc
     8401 ttggaacttc cgggtcgtag gagcttactt catgccggac atcgcattgc tcctgctact
     8461 ggccatcgtg ccagacattg tgcagggcat ctatatgata gcagctgcca tggcggaccg
     8521 ccccgccggc cgcgcggcca ggcctcgggg cagcagccgg cggtagaatt cggccaacgt
     8581 tgtcccagcc actatcgcgc atggtctttg ccagtctacc gaagttgaac cttcacagtt
     8641 catctagcaa ggcttgcgag aaggccctat cccttcccgc catcgcctcc tgagcttcca
     8701 tatttgtcga aaagatagtc caaggcctcg gcgtagccct cgaagccctt gcctactatc
     8761 accttttcgg cgtaaagcgc aacataggag aacttccgga agtcttccct cgatccgata
     8821 tcggtgaggt gtacctcgac gacgggcaga ctcacagcct taagtgcgtc aagtatggcg
     8881 atgttcgtga aggtgtagcc ggcagcgttc aagatgatgg cgtcgaatct ccccctggct
     8941 cgctgaatcg ccgtgataag cctaccctcg cagttggatt gcgaaacctg aacgaaaaca
     9001 cccttttgtt tgccctcttg ctttatgaac tccacaagct ccgagtaggt cctgtcgccg
     9061 taaagctgac gttccctgat gcccagcata ttcaggttgg ggcggttaag tacaagtact
     9121 ctcaccgcac tcttcctccc aaaatcacct tatcaggttt cgtttatcct ttcgagaatc
     9181 gccgtcgcgg cgccttccac agcggccggt tatggatctc gaagtcgcag aatctctcgc
     9241 gtacaagact tccctagatg ttctcgccgc tcgagcgcac ggcgtggctt gaggtcggtg
     9301 aagtgtgcgg ggaagacgtg gtcgcccgca ggggttccac gtctttccgc agaaaccctg
     9361 cgcacgtcta cgccttagtt ccattgtaga tcagaatggc gtccttgagg acagctgctg
     9421 tgccggacgg aaagatgact gctgaggcac aggaatatac ccccgggggt atccgcccgg
     9481 ggataaggtc acattctgac accagtttcg gcgaaaaacg caagttctga gtacatcgat
     9541 ttaggccggg gtccccgggg ggcgaatcga ccccaaaaaa ggagccctcg gggccagata
     9601 ccgccgattt agtggtgaag cgctggcgca acgtgccatc gcttacgtgc cgttaccaaa
     9661 tgaacccatg atgtgggata ttatgtcggt ccgcccgtct gcttcactgc ccaaacgcgt
     9721 cggatacccc ccggttttcg tgatttggag gcggtcaggt ggctcttcga aatcgatgta
     9781 ctcaaaagtt gcggggatct ccgaaactaa tagcaaaaac tgacactatg ccagctcccg
     9841 ccgtgtttga cccacagcca tcgcatctag gccagctgaa cggcctttcc gtcacgtctg
     9901 gtgaatctac acctctcagt acattggaca atcggcggaa cctcaatctg agaatgacat
     9961 tgctagacgg catatatggc atatgtcggg tggaaggctc ggttggtagc cgggccggtg
    10021 tcgctgactg ggtagatggc ccattttggt ccgcaacaca cacgcaggaa gagttgtcga
    10081 tcgtgtgcct gcaggagctg atacccgatc aggtcctgta tgaaggagat tggcgggtcc
    10141 ttaaggttct gggtcctctg agcttctcct tgaccggcat acttgcggcg atcagcagtg
    10201 tcttggctga agcaggcgtg agcatattct ccgtgtcgac gtacaacacc gactacattc
    10261 ttgtgaaata tgaccggctg gaagatgcta tggaggcgct gaccgggcga ggtgtagagt
    10321 tcatataggc cggccgcagc ctggcggagt tgtgcggaat gggatatgat gggcccatat
    10381 ggacgctcac ggcgccaacg tgcgaaatcg cggcactcga tcaaaggggt gtggcttaca
    10441 gtgaggggaa cagccaggag aagaccaaga atagcgaatg tcgtcttgat cggtctggca
    10501 actgtggtcg ttttgttctc ggtctgctcg atggtcattg tgaagaccat gtacgacaag
    10561 cagtttcaga gggccgacaa gcccagattc tcgggctacc ttacgtacgg cgatgtggac
    10621 ggataccacc gcagagctgt gcggttcaag tcgggcaaca acgctcttac gggttacgta
    10681 tacggtgagc gcaatgacaa gggattggtt gtcatcgccc atgggctagg gggcggcgcc
    10741 gaaagctatc tggcggaaac gatgtatttt gtggacaatg gctggcgtgt gttctcgttc
    10801 gactgtactg gaagccacga gagcgagggg aagggcacta tcgggcttcc acaatcagtg
    10861 ctggacctga attcagctct gagctacata gagagcgaca gctccctgga tggtctcccc
    10921 gctatgctct atgggcacag ctggggcggc tatgcggttg ctgctgtctt gaactatggc
    10981 cacgacatcg ccgcggcggc gagtatcgcc ggcttcaatg cacctatgga aatcctcttc
    11041 gagcgggcag agaagatgat ggggtcgatc gcatacctgg aatatccggt tctctgggca
    11101 taccagacga tgcttttcgg ccgcgccgcg cggcttacgg cagtcgacgg cataaacagc
    11161 gcggacactg cggttatgat catccatggc aacaaggaca caacagtgtc gtacaatagg
    11221 accggcgtca tagcgcatca gggcgagata acaaatccga acgtggtcta caagacgtgc
    11281 acggccgaga accacaatgg gcacagaaac ctatttcgat ctgaggcggc ggttgagtac
    11341 accaaccaga aaaaccgtgt ataccaggag ttacatgatc tctacaatgg cagcatacct
    11401 gacgacgtcc tggccgacta ctacgcgaat atagacaaaa ccctgaccag tgaacttgat
    11461 accggcttca tggacgagat caaccgcttc tttgagcgtt gtctgccgga ctgatcggtt
    11521 ctttggatgt ctccccgggt acgtccggtg gccccctgtg gggttgtggg gcatcctgtc
    11581 cgtttctgaa cacctttttc gggcatcctg ttcagatcct gcctggtttg gcgggggcca
    11641 tttgtcagaa ttcgcgccgg caacggcgcc ctcctttttc tggagccaag gcctcggaaa
    11701 aaccgcaggc agaggcgagt tgcataatac ggtcatacgc acacgccttc cgagtctcct
    11761 cggaaaggcc ccggagacgg gtttcagacc cctaatcact cccaaatcag ctgggccccg
    11821 gcttctgcct gtcaaaaccg ggcacattct gaacagcctg cgcagaaaac cggccgtttt
    11881 ccttctacac gttacccata tccatggatt ggcccggata caaaggccga agcgccaagg
    11941 atgtccacat attgcagctg agcgggaaac tgctgacgcc gtaccatggg tacccagacc
    12001 gcataagctg actgactacc gggccggcga cctgcttagg ctcggaaacg atagttacag
    12061 gcgaaaaagg ctttgcacag gtctttggta aaacgggcgt ttagcagggg attagcgagc
    12121 ctacaacaaa tgtgggtggt tttccgacgt acctccttgg aatgtgcgag gttttgacgg
    12181 ctaggcgaag agggccggac caatatgggt tttcttagta gatcaagggc tgcctgaggg
    12241 ctttttccgg tgcgctcgag aacctcacac gcgtatccat gcctgagccg aatcggccgg
    12301 ccgcaaccag gggtatgtcc ccgagc
//
LOCUS       DAOTAC010000036        12305 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_4363_length_12305_cov_4.663673, whole genome shotgun sequence.
ACCESSION   DAOTAC010000036 DAOTAC010000000
VERSION     DAOTAC010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 12305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12305
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4363_length_12305_cov_4.663673"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1318)
                     /locus_tag="PLM74_02710"
     CDS             complement(<1..1318)
                     /locus_tag="PLM74_02710"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HON42030.1"
                     /translation="MRTGVQTRLLASYLVLLILMIAVGGVGILSDRRVENEYVELVHS
                     NLPITILLHKLREGLLEKGMAARGFVLYQDNKLVDQFYTAEKTIKANIEAIAELDPSE
                     ESLNYLDQIDQTNKQYTRVCEHALDLARCQEIEDARDLLAEQAPALIDEFDRLTAEWF
                     AFLDGRNNDSLLRAKEESRRSTWLTAGAVLISVIVVMIMATSVARTVAVPVAKMRAAA
                     EAVAAGDLTVPLPTIKTRDEMRDLNEAFRVMISNLTRLLGDMRAESDKVAGASRELST
                     STEESARAVGQITDTIQQMAAGAEQQSASAGRTVSAGEQIKAGIEQVASGAGEQTRQL
                     HRASELVAQMVNELEQISAYLGQMEKDMRATVDASKEGDRCVTRAAQSIARIKEASAE
                     VESASAGLGKSSREIGRVVQVIGDIADQTNLLALNAAIEAARAGEQG"
     gene            complement(1319..3229)
                     /locus_tag="PLM74_02715"
     CDS             complement(1319..3229)
                     /locus_tag="PLM74_02715"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HON42031.1"
                     /translation="MRLGIRAKLIGSYALLLALSITVNLVGMWADKRADESNRYVIEQ
                     TLPVSALVYKARSELHEKGANLRSFIITSDEAHAMHFYNTDAKMAETIDAARSAFPDE
                     RSADYLDEFESHNAEYNVIADDIMMMVRMGDNEEAGAMLATGIEPLALTIDHLVADWS
                     EFVEERNAEWLAAATQTNTIATLVSIAGAAVSAVAVIIIAAILIRGIAVPVAQVKRVA
                     EAVAQGDLTVSMPVIRTRDEMQDLNEAVHTMLQNLVGLLGDMRTDANNVAGASHELST
                     SAEGSACTVAQITATIQQMAAGAEQQSAGADRTVSAGEQIKAGIEQVAAGAGEQTRQL
                     HRASQLVAQMVNELEQISAYLGQMEKDMRATVNASKGGDRCVTKAAQSIARIKEASAE
                     AESASVGLGKSSREIGRVVQVIGDIADQTNLLALNAAIEAARAGEQGRGFAVVADEVR
                     KLAERSLAETKAISKLIEVTVADTGKVSAAIEISSKLVEESMPLVKASMSSLKKIGEH
                     ASNNLTLVDSAVKLRQSVMDAAGTVRESMVEAVAVADENAAAAAQMSVGIAEVQKSIE
                     SVAAVSEENAAAVREVSASAEEVSASIAHIASASQSLDQMASRLQELAAKFRTVHDEH
                     DDAVFIEERRIS"
     gene            complement(3400..5262)
                     /locus_tag="PLM74_02720"
     CDS             complement(3400..5262)
                     /locus_tag="PLM74_02720"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HON42032.1"
                     /translation="MKLSIRAKLVGSYALLLALIVAVNLVGIWADRQAAANNHSVIEQ
                     SLPASALVYRVRSEVFEKGTAVRGFMITLDESNITKFYDINNTMMDTLNAARETFVNE
                     ESYRYLDEIMSTNDAYNNLVNEVMIMARVGKTEEAMARLTADGQELLNQFDSLIADWS
                     AFVSDTNQQWLADAARASRMATLISIAGTVISAAVMVIVAASLVRGITVPIVRVRHVA
                     EAVAAGDLTVSVPAIRTGDEMQDLNEAVRTMIRNLTALLGEMRIESDNVASASYQLSA
                     SSDESARAVEQITATIQQMAGGAEQQSASATRTASAGEQIKSGIEQIARGAGDQTQQL
                     HQASGLVAQMVEGLEQISAYLGQMDKDMRSTADAAEEGDRYVGQAAQSIARIKEASAE
                     VESASAGLDKSSREIGRVVQVIGDIADQTNLLALNAAIEAARAGEQGKGFAVVADEVR
                     KLAERSLAETKAISKLIEATMADTGRVSKAIETSGELVEESMPLVEASMSSLKKIRQH
                     ASDNLTLVGSAVKLRQSVMDAADKVKQSMVEAVAVSDENAAAAEEMTAGVAEVQKSIE
                     NVAAVSEENAAAVEEVSASTEEVSSSIEDMASASQSLAAMANRLKELAARFRTA"
     gene            5470..5545
                     /locus_tag="PLM74_02725"
     tRNA            5470..5545
                     /locus_tag="PLM74_02725"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5504..5506,aa:Glu,seq:ttc)
     gene            5846..8200
                     /locus_tag="PLM74_02730"
     CDS             5846..8200
                     /locus_tag="PLM74_02730"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HON42033.1"
                     /translation="MATGSGIRQIAGSLRFKLLILILLAVLPLLAFSVYMAMNERSEA
                     LEQAEYQSRLLLTHIVGADSEDIGNLLRVAAVVWDASLPHTREESDPLRLTGVLSESW
                     HSRRQTSSFAVLDGTGRVLAATWGPDGPSDLGTSSAFREAREYGRPFAEVTDSNPFGS
                     STAMAIYIPSAARSRSSDNDSLDDARAILRRHGGQMAAVFLHPEWFAESFRDLALPEG
                     ATLAVHDMTGRNLMRYRKDEGNDVPRDDEGTSMCDHEVLNAVRSSRYDVGSVTSTGCD
                     GLQRLISYGKVGSSNDDPQFYVTMSVPVAMIEVAAKREVAYTFAAVAAVVLIVVPLSW
                     SFAKRQVIAPAGSLARAADHLASGDLATRYDGPRVSGELGELAYAFDRMAETLEERTV
                     QLEYLIYHDSLTGCYNRPYFDHVLSAFEDESKLPMTFVLADVNGMKQVNDLLGHEAGD
                     ELLKTMASIIVGAVGDEGMVFRWGGDEFLVVMANVERRTALRLASRVRKAVGQLNVGT
                     VSASIALGAGTRISLVQSVTETIQRAEGRMFRNKFMEMTSPRGTLVHSLRKALAESTH
                     ETEEHSSRMRFLAVELAHTLGLSDNSIDDLSLLCVLHDIGKVGIPDQILRKSGPLSPE
                     EWKVMRTHPEIGARIVEGSAELSHVANAILSHHERWDGRGYPRELEGAEIPLAARMLA
                     IVDAYDAMVNDRPYRRALSHTEAMEEIQRNSGTQFDPDLVQAFAEMMEALEGYKAYES
                     AWIRAEQFIFEEDIGWNDSAEAEAAVGGGGNGLAEGGDSGNGQG"
     gene            8365..9942
                     /locus_tag="PLM74_02735"
     CDS             8365..9942
                     /locus_tag="PLM74_02735"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HON42034.1"
                     /translation="MFKRITLSILAALLLVSASASVFGATPVKGGTLKIAASSIQQLD
                     PYKTAANDETNICGLVFDPLVIIGKDNFKPRPHLAQKWETPDANTWIFHLRKGVYFQE
                     GNEVFKKGAKREVTADDVVYSIERFLKVSTAFTLGDIKSVKALDRYTVEIKTPVPNPF
                     LVADPNRIASVGIVPREAIEKLGEDGFARRPIGSGPFALKSFATDQGAVLERNPNYWL
                     PVYLDKVEFVVIPDPTVQILALTAGEVDIVPYLFNLDSMASVAKNPKLATLSRGGSYR
                     GLGFNVTKPPFDELEVRDAISKAMDIDAAFNAVVAPHGERAYGQCAPWLPHGYDTSLK
                     SLWKYDPKEAVAILNKAGFRDTNGDGILDRNGKPLKVEIKTIAGSQVRTLTILATQLR
                     KIGIDASILQQDTAVWASDIIDGNTEVFFDFSFAGTTGLHSLFHSSSIGKTNGHYYSN
                     ATVDSLLDQALKTTDYTKLTSLWKQAQRKIMEDRVSIPLYFEWGYSIVNKRVNDFVPP
                     WGGMHLVTLENSVWISK"
     gene            10061..11008
                     /locus_tag="PLM74_02740"
     CDS             10061..11008
                     /locus_tag="PLM74_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011228608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HON42035.1"
                     /translation="MGKYIVRRLLATIPVLIGITFITSAMVSLAPGDAVKLMLGSETA
                     NAANVERLQKELGLDRPWYEQYFRYMAGLLKGDMGRSIVYHRPAADQIKEKLGNTIVL
                     AISSTIFALVVSIPLGVLAAAKRRSWVDYAATTLAMIGVSLPGFYLGLLMILFFGVKL
                     GWLPIRGMGVTGAGLWAYVRHLIMPSITLGSALAAILTRLTRSSVLEALSQDYVRTAR
                     AKGVHEKVVLYKHALRNALLPVATTFGLQFGSLLGGAVITETIFSWPGIGMLAVNAVK
                     QRDIPVIQGTTLVFALSFVLVTLAVDILYMLIDPRIRYE"
     gene            11001..11879
                     /locus_tag="PLM74_02745"
     CDS             11001..11879
                     /locus_tag="PLM74_02745"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HON42036.1"
                     /translation="MSKEAKGTIRSEFAHVVRVFFRHKLGVAGLVMLIVFAGAAAFAP
                     VVAPRDPYEMDLASPFLPPGSPGYILGTDNYGRDILSRLIHGSRISLLIGIVVVSIAT
                     VIGSILGLVAGYYGGWVDNLVMRLVEVFYSFPFLILVIGVMAILGPSIFNVMLVLGVV
                     SWPLYARLVRAQVMALKSEDFVKAASALGCSDARIMFRHILPNVLTPVIVSATLGIPG
                     AILSSASLGFLGFGVQPPTPEWGAMVSEAKDFILLNSNMIIWPGLCIFLVVLSFNFVG
                     DALRDALDPRLAKQLN"
     gene            11989..>12305
                     /locus_tag="PLM74_02750"
     CDS             11989..>12305
                     /locus_tag="PLM74_02750"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HON42037.1"
                     /translation="MSKSISIDDRLAKVPDYTRFLTVDELYERARTLAEKHPEIAKLT
                     IVGKSTAGSEIPMVSIGNGPASILVFACPHPNEPIGAMTTCFLMDELVADDNLRSGRT
                     WHI"
BASE COUNT         2383 a         3641 c         3578 g         2703 t
ORIGIN      
        1 tgccctgctc cccggcgcgg gcggcctcga tggcggcgtt tagagctaga aggttggtct
       61 ggtctgcgat gtcgccgatt acctgtacca cccggccgat ctcgcgggag ctcttgccga
      121 ggcccgccga tgcggattcg acttcggcgg aggcttcctt tatcctggcg atgctctgcg
      181 cagctcgggt cacgcagcga tcgccctcct tcgaagcatc cacagttgcg cgcatgtcct
      241 tctccatctg gccaaggtac gcgcttatct gctcaagttc attcaccatc tgcgccacaa
      301 gctcggacgc ccgatgaagc tgccgcgtct gctcgcctgc gcccgacgca acctgttcga
      361 tgccggcctt gatctgctcg ccggcgctca cggtccggcc agcacttgcg ctctgctgct
      421 cagcgcctgc ggccatctgc tgaatcgtat ccgtgatctg ccccaccgcg cgcgccgatt
      481 cttcagtcga tgtggacagc tcacgactgg cgccggcaac tttgtcggat tcagcgcgca
      541 tatcacccaa gagccttgtg aggttgctga tcatcacgcg gaaggcttcg ttgaggtcgc
      601 gcatctcatc gcgggttttg atcgtcggca atggcacagt caaatcgcct gcggccactg
      661 cttcagccgc tgctctcatc ttggccaccg gcacagcgac agtgcgcgcg acggacgtgg
      721 ccatgatcat tacgactatc acgctgatga gcaccgcccc ggcggtgagc cacgtactgc
      781 ggcggctctc ttctttggcc cgtagcagtg aatcattgtt gcgcccgtca aggaacgcga
      841 accattcagc tgtcagtctg tcgaattcat cgatgagcgc cggcgcctgc tccgcgagaa
      901 ggtcccttgc gtcttcgatt tcctggcatc tggccaggtc cagggcatgc tcacatactc
      961 tcgtgtactg cttgttggtc tggtctattt gatcgaggta attgagggac tcttcgctcg
     1021 ggtccagctc ggctatggct tcgatgttgg ccttaatcgt cttctcggcc gtgtagaatt
     1081 ggtccacaag cttgttgtcc tgatacagga caaaaccccg ggcggccatg cccttttcca
     1141 gcagaccctc gcgcaacttg tgcagcaaga tcgttatggg taggttggag tgcacaagct
     1201 ccacgtactc attctccacc cgcctatccg acaggattcc taccccgccc actgcaatca
     1261 tgaggatgag caagacgagg tacgatgcca acagcctggt ctgtacaccc gtgcgcatct
     1321 atgagatcct cctttcttcg atgaaaaccg catcatcatg ttcatcatgc accgtcctga
     1381 acttggcggc gagctcctga aggcgactgg ccatctggtc aagggactgg gacgcggacg
     1441 ctatgtgcgc gatggaggcg cttacttcct ctgccgaggc ggagacttcc ctgactgcgg
     1501 cggcgttttc ctctgatact gccgccacgc tctcgatgga cttctggact tcagcgatgc
     1561 cgacactcat ctgtgccgcc gcagccgcgt tttcgtcggc cactgccaca gcctcgacca
     1621 tcgactcccg gaccgtgccc gctgcgtcca tcacgctctg tcgcagcttg acagctgagt
     1681 cgacgagcgt caggttgttg gaggcatgct cgccgatttt cttcagcgac gacatggagg
     1741 ctttgaccag cggcatactc tcttcaacca gcttgctcga gatctctata gcggcagata
     1801 ctttgccggt gtcggccacc gtgacctcga tcagcttgga tatcgccttc gtttccgcaa
     1861 gcgaccgctc tgccagcttc cgcacttcat ctgcgaccac cgcaaatcct ctgccctgct
     1921 ccccagcgcg ggcggcttcg atggcggcgt ttagagctag aaggttggtc tggtctgcga
     1981 tgtcgccgat cacctgtacc acccggccga tctcgcggga gctcttgcca aggcccaccg
     2041 aggcggattc ggcttcggct gaggcttcct ttatcctggc gatgctctgt gcagccttgg
     2101 tcacgcagcg atcgccgccc ttcgatgcat ttacagttgc gcgcatgtcc ttctccatct
     2161 ggccaaggta ggcgcttatc tgctcaagtt cattcaccat ttgtgccaca agctgggacg
     2221 cccggtgaag ctgccgagtc tgctcgcctg cgcccgctgc gacctgctcg atgccggcct
     2281 taatctgttc gccggcgctc acggtccggt cggcgcctgc gctctgttgc tcagcacctg
     2341 cagccatctg ctgaatagtg gctgtaatct gcgccaccgt gcacgcagaa ccctcggccg
     2401 acgtcgacag ctcatggctg gcgccggcaa cgttgttggc gtcggtccgc atatctccaa
     2461 ggagccccac caggttctga agcatggtgt ggactgcctc gttgaggtcc tgcatctcgt
     2521 cgcgggtcct gatgaccggc atggataccg tgaggtctcc ctgggcgaca gcctcagcga
     2581 cacgtttgac ctgcgcaacg gggacggcaa tgccgcggat cagaatcgcc gcgatgatta
     2641 tcacggccac ggcgctcacg gcggcgcctg ctatgcttac cagggtggct atggtattgg
     2701 tctgggtagc agcggccaac cactcggcat tgcgttcttc tacgaactcg ctccaatctg
     2761 cgaccagatg gtcaatggtg agcgccaaag gctcgattcc agtggccagc atggccccgg
     2821 cttcctcgtt gtctcccata cgaaccatca tcatgatatc gtctgctatc acgttgtact
     2881 cggcattgtg gctctcgaat tcgtccagat aatcggccga cctctcatca gggaaagcgc
     2941 ttcgagcagc atcgatggtc tcagccatct ttgcatccgt gttgtagaaa tgcatcgcat
     3001 gggcctcatc tgacgtgatg atgaagcttc gcaagttggc gcccttctcg tgcagctcgc
     3061 tgcgagcctt gtacaccagc gcagacacgg gaagcgtctg ctcgataaca tacctgttgc
     3121 tctcatcagc ccgtttgtcg gcccacattc cgactaggtt cacagtgata gacagtgcga
     3181 gcagcagcgc ataggagccg atcaacttgg cccgtatgcc gagtctcatt tagcaaccct
     3241 cccgacaaat ccgtgagctt caggacgtgt atactaaata tatcggcatg aactggtcca
     3301 gacgtgagag ttcttggtgg tctcccggac tcggacatgc ggaagcatcc tgactcaatc
     3361 gggacgccag agagacaggg cgtttcccct gctgagcccc tacgccgtcc tgaatctagc
     3421 tgcgagttct ttgaggcggt tggccatggc ggcaagcgac tgggaggcag acgccatgtc
     3481 ttcgatcgag gagctcactt cctccgtcga tgcagagacc tcttccactg ctgccgcgtt
     3541 ctcttctgat actgctgcca cgttctcgat ggacttttgg acctcggcca cgccggcggt
     3601 catctcttct gccgcggcgg cgttctcgtc ggacacagcc accgcttcga ccattgattg
     3661 ctttaccttg tcagctgcat ccatgacgct ctggcgcagc ttgacggcag agcccaccag
     3721 ggtaaggtta tccgatgcgt gctgacggat cttcttcagc gacgacatag aggcctcgac
     3781 caggggcatg ctctcttcaa ctagctcgcc cgaggtctct atggccttgg acactctgcc
     3841 cgtgtcagcc atcgtcgctt cgatcagctt ggatatggcc tttgtctctg caagcgaccg
     3901 ctccgcgagc ttgcgcactt catccgcgac caccgcaaag cccttgccct gctctcccgc
     3961 gcgggcagcc tctattgcgg cgtttagtgc gaggaggttg gtttggtccg cgatatcgcc
     4021 gattacctgc actacccggc cgatctcgcg cgaactcttg tctaggcctg ctgaggcgga
     4081 ttcgacttca gccgaagctt cctttatcct ggcgatgctc tgggccgcct ggcctacgta
     4141 gcggtcaccc tcttctgctg catctgctgt cgaccgcatg tccttatcca tctgacccag
     4201 ataggcgctt atctgctcga gtccctcaac catctgcgcc acaagcccgg aggcctggtg
     4261 gagctgctgg gtctggtcgc ctgcacctct agcgatctgc tcgatgcccg acttaatctg
     4321 ctccccggcg cttgccgtcc gtgtcgcgct ggcgctctgc tgctccgcgc cgccggccat
     4381 ctgctggatt gtggccgtaa tctgctcgac cgcgcgcgcc gattcgtcag acgacgccga
     4441 cagctggtaa ctggcgctgg ccacgttgtc ggactcaatg cgcatttccc cgaggagtgc
     4501 cgtgaggttc cggatcattg ttcgcactgc ctcattcaga tcctgcatct catcgccggt
     4561 cctgattgcg ggtacagaca ctgtgaggtc gcctgccgcg acggcctccg ccacatgtct
     4621 gacgcgcact atagggacgg ttatgccgcg gacgaggctg gcggcgacga tgaccatcac
     4681 ggctgcgctg atcacagtcc ctgctatgct gatcagggtg gccattctgc tggccctggc
     4741 agcgtcggcc agccactgct ggttggtgtc gctgacgaac gcgctccaat cagcgatcag
     4801 gctgtcgaat tggttaagca gctcctggcc atcggctgtc agtctggcca tggcttcctc
     4861 ggtcttgcct accctagcca ttatcattac ttcattgacc aggttgttgt aggcgtcgtt
     4921 tgtgctcatg atctcgtcga ggtatctgta ggattcctcg ttcacaaacg tctcgcgggc
     4981 cgcattaagt gtgtccatca tggtattgtt aatgtcatag aacttcgtga tgtttgattc
     5041 atccagtgtg atcatgaaac ctcgaactgc tgttcctttc tcaaagacct cgcttctgac
     5101 cctgtacact agcgcggatg ccggcaagct ctgctcgatg acactatggt tgttcgcagc
     5161 agcctgtcta tcggcccata ttcccaccag attcactgcg acaatcagcg cgagcagaag
     5221 cgcataggaa cccacgagtt tggctctgat actcagcttc aattggatat ccctccgtag
     5281 accggcaagt gatgagtcca gactgttcac atacaattat cggcatgatt cgataagaaa
     5341 taaggtcaaa gctgcaagcc taacgggatt ccgagagccc ggatcgccgc gggggtgagt
     5401 ttgacgaaag ccggcgctca tgttatcatt actgagcaaa actacaggcg gtcaatgcgg
     5461 agagatttgg ctcccatggt ctagcggcct aggacatcgc cctttcaagg cggtaacacg
     5521 ggttcgaatc ccgttgggag cgccatattg atagagaggc cccgcttcaa gcggggcctt
     5581 gatgcatggt gtggggtcga ttggtcaaca ctgcggtgcc cgcagcagcg cggcgcaggg
     5641 ggactatgtg gagggctcgg tggggcccgg tgccctgcgg ctgcaaaata caacaaatag
     5701 tggcattcct tgatcgaatc tgctatggtt gttgatgggt gcactattcc tcggtcccag
     5761 tcgaaagtga ccatcacgtg gagcactcgg gagtgatcga acaagtggcc ggcacttgcg
     5821 gatctctccg tagagggggt atacgatggc cacgggcagc ggcatcaggc agatcgcagg
     5881 tagcctgcgg ttcaaactgc tgattctcat tttacttgct gttcttccgc tccttgcctt
     5941 ctctgtatac atggccatga atgaacgctc cgaagcactt gagcaagccg aatatcaatc
     6001 gcgcctgctg ctcacccata tcgttggcgc agatagcgag gacattggaa acctacttcg
     6061 ggtggcagca gtagtgtggg atgcctcgtt gcctcacacc cgtgaggaat ctgacccatt
     6121 gcgacttacg ggtgttcttt cagagtcgtg gcattccagg aggcagactt ccagcttcgc
     6181 cgtgctcgat ggcacagggc gagtgctagc tgctacatgg ggccctgatg ggcctagcga
     6241 cttaggaacg tcctccgcgt tccgggaggc cagggagtac ggacggcctt tcgccgaggt
     6301 gacagactca aacccatttg gaagctcgac tgcaatggcc atatacatcc cgtcggcggc
     6361 gcggtcgcgc tcatccgaca atgacagtct cgatgatgcc agagcgatac tgcgacgaca
     6421 cggcggccag atggctgccg tttttcttca ccctgagtgg tttgcggaat cattcaggga
     6481 cctggctctg cctgaagggg cgacgctcgc tgtacacgac atgacgggca ggaacttgat
     6541 gcggtacagg aaggacgagg gcaacgatgt gccccgcgac gatgaaggta cgtccatgtg
     6601 cgaccatgag gtgctgaacg ccgtaagatc tagcagatat gatgtgggat ccgtcacatc
     6661 cacgggctgc gatgggctgc agcggctgat atcctacggc aaggtaggca gctcaaatga
     6721 cgacccgcag ttttacgtga ctatgagtgt tcccgtcgcg atgatagagg tcgccgccaa
     6781 acgagaggtt gcgtacacct tcgctgcggt ggctgcagtt gtgctgatcg ttgtgccgct
     6841 ttcgtggtct ttcgcaaagc ggcaggtgat cgcgcctgcc ggcagcctag ctagggcggc
     6901 ggatcacctg gcgtccgggg atctcgctac tcggtatgac ggtccgcggg tcagcggcga
     6961 actcggcgaa ctggcctacg ctttcgaccg gatggccgag actctggagg agcgcacggt
     7021 ccagcttgag tacctcatat accatgacag cctcaccggc tgctacaacc gtccctactt
     7081 cgaccacgtt ctttccgctt tcgaagacga gtcgaagttg cccatgacat tcgttctggc
     7141 tgatgtgaac ggcatgaagc aggtcaacga ccttctcggt cacgaggcag gcgatgagct
     7201 gctcaaaacg atggcgagca tcatcgtggg cgctgtcggc gacgagggca tggtattccg
     7261 atggggcggc gacgaattcc tcgtggtgat ggccaatgtt gagcgaagga cggccttgag
     7321 gctcgcttcc agggtacgca aggctgttgg gcaactgaat gtggggacag tctcagccag
     7381 cattgctttg ggcgcgggca cacgcatttc cctcgtgcag agcgtgaccg aaaccattca
     7441 aagggcagag ggccggatgt tcaggaacaa gttcatggag atgacctcgc cccgcggtac
     7501 cctggttcat tcgctgcgta aggcgcttgc ggagagcact catgaaacag aagagcattc
     7561 cagccgcatg cgcttcctgg cggtggaact agcccatact ctggggcttt cggataattc
     7621 catcgacgac ctttcgctgc tatgtgtgtt gcacgacata ggcaaggtgg gcatccccga
     7681 ccagattctg cggaagtcag ggccactgtc gccggaggaa tggaaggtca tgcgcaccca
     7741 tcccgagata ggcgcgcgaa tagtcgaagg atccgccgag ctttcgcatg ttgccaacgc
     7801 cattctctca catcatgagc gatgggacgg cagggggtac ccccgcgagc ttgaaggcgc
     7861 ggagatcccg cttgccgcta ggatgctggc aattgtcgat gcgtatgacg ccatggtgaa
     7921 tgataggccc taccgcaggg cattgtcgca cactgaagcc atggaggaga ttcagcgcaa
     7981 ttcagggacg cagttcgacc cagatctcgt gcaggccttt gctgagatga tggaggcgtt
     8041 ggaagggtac aaggcatacg aaagcgcgtg gattcgcgcc gagcagttca ttttcgagga
     8101 ggacattggg tggaatgact ccgccgaggc cgaagccgcc gtcgggggcg gaggcaatgg
     8161 actagcggaa ggcggagaca gcggaaacgg acaaggttga cattgcgaat agtaatccaa
     8221 tggaatatgc gcgcctcttg ccatcgcatg gtagatgccg agtgagatgc cggatgagct
     8281 tgacttaggc atgtgccatg cgtataatca accttataca aattagcagg ttaggacttc
     8341 agacgtggca ggaggtaaca ccgcatgttc aaaagaatca ctctatccat tctcgcggcg
     8401 cttcttctgg tctctgcctc cgcatcagtc ttcggggcaa caccagtcaa gggcggcaca
     8461 ctgaagattg ccgcatcgtc tatccagcaa cttgacccgt acaagactgc tgccaacgac
     8521 gagaccaaca tctgcgggct cgtattcgat cctctcgtca tcatcggcaa ggacaacttc
     8581 aagcccaggc cgcacctggc gcagaagtgg gagactcccg acgcgaacac gtggatcttc
     8641 catcttcgca agggcgtgta cttccaggaa ggaaacgaag tcttcaagaa gggcgcgaag
     8701 cgcgaggtca cagcggacga cgttgtgtac tcgatcgaac gcttcctcaa ggtgagcacc
     8761 gcattcaccc tcggcgatat caagagtgtg aaggcgctgg accgctacac ggtagagatc
     8821 aaaacgcccg ttcccaaccc atttctagtg gccgacccca atagaatcgc cagcgtcggc
     8881 atcgtgccgc gcgaggccat tgagaagctg ggcgaagacg gattcgccag gcgccccata
     8941 ggctccggtc cattcgcgct caagagcttc gcaactgatc agggcgcagt gctcgagcgc
     9001 aaccccaact actggctccc tgtgtacctg gacaaggtcg agttcgtggt catccccgac
     9061 cccacagtcc agatacttgc acttactgca ggcgaagtcg acatagtccc atatctcttc
     9121 aatcttgatt cgatggcatc cgttgccaag aaccccaagc tcgccacttt gagcagaggc
     9181 ggatcataca ggggtctcgg gttcaacgtg acgaagcccc cattcgacga gcttgaagtg
     9241 cgtgacgcca tatccaaggc catggacatc gacgcggcgt tcaacgcagt agtggcgccc
     9301 catggcgagc gtgcatacgg tcagtgcgcg ccgtggctgc cccatggcta tgataccagc
     9361 ctgaagagcc tgtggaagta cgatcccaag gaagcggtgg ctatcctgaa caaggccggc
     9421 ttccgcgata ccaacggcga tgggatcctc gaccgcaacg gcaagccgct caaggtggag
     9481 ataaagacta ttgccggttc ccaggtgagg acgctcacta tcctggcaac ccagctccgg
     9541 aagatcggaa tagacgcatc catcctccag caggacacgg cagtgtgggc atcggacatc
     9601 atcgacggca acaccgaagt cttcttcgac ttcagcttcg ccggaacgac cgggctgcat
     9661 tcgctcttcc acagcagctc gattgggaag accaatggtc actactactc gaatgcgact
     9721 gtggactcct tgctcgacca ggcgctcaag accaccgact acaccaagct gacgagcctt
     9781 tggaagcagg cgcagcgcaa gatcatggaa gatagggtgt ccatccctct ctacttcgag
     9841 tggggttatt cgattgtgaa caagagagtg aacgacttcg ttcctccgtg gggcgggatg
     9901 catctagtca ccctcgaaaa cagcgtctgg ataagcaagt agcgtcgacc gcggctaccg
     9961 cgttgcagct atggcgggga gggcgtcgct ttccgggcgc gaggctacgg aaacggaagg
    10021 cgatgtccct cccttccggg gttagtgggg agtgtcggaa ttgggcaaat acatcgttcg
    10081 tcgcttgctc gcgacgatac cggttctcat agggataaca ttcataactt cggcaatggt
    10141 tagcctggcg ccgggcgatg cggtcaaact gatgctgggc tcggagactg ccaatgcggc
    10201 caacgttgag cgcctgcaga aggagcttgg cctcgacagg ccatggtatg agcagtactt
    10261 ccgctacatg gcgggtctgc tcaaagggga catgggaagg tctatagtct accatcgtcc
    10321 ggcggctgac cagatcaagg agaaactcgg gaacaccata gtcctcgcaa tcagctccac
    10381 gatcttcgcg ctcgtggtgt cgatccccct cggcgtgctg gctgccgcca aacgacggag
    10441 ctgggtagac tacgccgcca cgactcttgc gatgataggg gtatcgcttc ccggcttcta
    10501 cctgggcctt ttgatgatac tgttcttcgg cgtcaagctc ggatggcttc ctatcagggg
    10561 gatgggggtc acaggggcag gcctctgggc ttacgtgcgc cacttgataa tgccttcgat
    10621 tacactgggc agtgcgcttg cggccatact gacccgcctt accaggtcgt cggtactgga
    10681 ggctctgtcg caggattacg tgcgcaccgc tcgcgccaag ggagtgcacg agaaggtggt
    10741 cctctacaag catgctctgc ggaacgcgct gctgccggtg gccaccacct tcgggctgca
    10801 gttcggatcg cttttaggcg gagcagtcat tacggagacg atcttctcat ggcccggcat
    10861 cggcatgctg gcggtaaatg ccgtgaagca gcgggacatc cccgtaatcc aggggacgac
    10921 gctcgtgttc gcgctgagct ttgtccttgt cacccttgcg gttgacatac tatacatgct
    10981 aattgatccg aggattcgat atgagtaaag aagcgaaagg tacaatcagg tcggagtttg
    11041 cgcacgtcgt tcgggtgttc ttccggcaca aactgggcgt cgcgggcctt gtgatgctga
    11101 tcgtgttcgc cggcgccgcg gcgtttgctc ccgtggtcgc gcccagagat ccgtatgaga
    11161 tggacctggc gagccccttt ctgcccccgg gctctcccgg ctacatcctc ggcactgaca
    11221 actacgggcg ggacattctg tctcggctga tacacggttc gaggatatcc ctgctcatag
    11281 gcatcgtcgt agtatcaata gccaccgtga tcggctcgat actgggcctc gtcgcaggct
    11341 actacggcgg ttgggtcgac aacctggtca tgcgtctggt ggaggtcttc tactccttcc
    11401 cattcctgat cctggtgatc ggggtcatgg ccattctggg gcccagcatc ttcaacgtca
    11461 tgttagtatt gggcgttgtc agctggccgt tgtacgcgcg cctagtgagg gcgcaggtaa
    11521 tggcgctcaa aagtgaagat ttcgtcaaag ccgcaagcgc cctggggtgc agcgacgccc
    11581 gcatcatgtt caggcacata cttccgaacg tgcttacgcc ggtgatagtc tcggcgacct
    11641 tggggatacc cggggctata ctctcatctg cgtccttggg gttcctgggg ttcggggttc
    11701 agccccccac cccggagtgg ggcgccatgg tcagcgaggc gaaggacttc atcctcctga
    11761 actccaacat gattatctgg cccgggctgt gcatattcct cgtggtgctg agcttcaact
    11821 tcgtcggcga tgcactgcgc gacgcactcg acccgaggct ggccaagcag ctgaactgac
    11881 ggatcagggc agtaaggggc agtaagacaa cggagcaaga agcacgaaag tcacattgca
    11941 cagatcgaga atcacattga tgcacgatta ggattaggag gattgcttat gagcaagtcg
    12001 atttccatag atgaccgtct ggccaaagtg ccggactaca ctcggttctt gacagtagac
    12061 gagctctacg agcgggctcg gacactcgcg gaaaaacatc ccgaaatcgc gaagctgacc
    12121 atcgtgggca agtcaacggc gggtagcgag atacctatgg tgtctattgg caatggcccg
    12181 gcgagcatcc tcgtgttcgc atgccctcat ccgaacgaac ctatcggggc catgacgacc
    12241 tgcttcctca tggatgaact cgtggccgac gacaacctgc gctccgggcg cacttggcac
    12301 atcat
//
LOCUS       DAOTAC010000037        12303 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_4364_length_12303_cov_4.781352, whole genome shotgun sequence.
ACCESSION   DAOTAC010000037 DAOTAC010000000
VERSION     DAOTAC010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 12303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12303
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4364_length_12303_cov_4.781352"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..905)
                     /locus_tag="PLM74_02755"
     CDS             complement(60..905)
                     /locus_tag="PLM74_02755"
                     /inference="COORDINATES: protein motif:HMM:NF012632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42038.1"
                     /translation="MSGLEDIVAVAAGANHSLALRADGTVWAWGANDRGQLGNGMYSD
                     RCDVPAHVAGLSGVRAIAAGECHSLAVRDDGTVWEWGDNSTYQIGHGAPTTTPAPVMK
                     AGMDRVESVAASGYRSLALKTDGTVWQWGFWQLALPWGDSVYWLNRYVGDAESVGLHG
                     VTSIAAGSDHSMALKDDGTVWAWGVNIHGQLGGGTKWDRDPLRVEGLDEVVAVTAGDR
                     NGLAVRADGSVWAWGAGAQNYLGTGTYSAIAPPTMVMGISGVADVASGEQIIVAADCF
                     GDLWR"
     gene            complement(948..1358)
                     /locus_tag="PLM74_02760"
     CDS             complement(948..1358)
                     /locus_tag="PLM74_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42039.1"
                     /translation="MRRAFGLLLIALCVLMAGCGLPVGISYTVSRRIVCADDPSQGIG
                     DVYISFSGSRSFGTAKTNDDGTWTKSGLRGSVTIAPADADWVFEPGYRQVSGAEQNVS
                     FAGVPSASNSLGFRQVVAGRNYALFLRADGTVYF"
     gene            complement(1451..4789)
                     /locus_tag="PLM74_02765"
     CDS             complement(1451..4789)
                     /locus_tag="PLM74_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020507743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Swt1 family HEPN domain-containing protein"
                     /protein_id="HON42040.1"
                     /translation="MAVTNHERVGEALDLLKEGLAPFVERELRARYGKYWITQATASW
                     PNDVNWTGDDDQPHLDCAVLLRLMWDQWNEVFRNTLGHAERSLVSELREVRNKWAHQD
                     RFSSDDAYRALDSASRLLTAVSAPQAADLDRIKMNLLRVRFEEQMRTEQRKSSGTAVQ
                     SAAMGSLRPWREVATPHPDVASGHYQQAEFAADLWQVHLGEGGDEYRDPEEFYRRTYL
                     TESLKHLLVRGVQRLAGTGGDPVVQLQTNFGGGKTHAMLALYHLMSGTPSGDLLGVDD
                     VLREAGVDKLPTARRVVLVGNKLSPGNPVTKPDGTVVRTLWGELAWQLGGREAFERVR
                     ADDENATSPGDMLRELFNKYGPCLILIDEWVAYARQLHDQSDLPAGSFETQFSFAQAL
                     TESAKLARQCLLVISLPASDTGASPHAQADDAEVGGHRGREALNRLRNVVGRVESSWR
                     PASAEEGFEIVRRRLFEPLVSQEQFVGRDTVARAFYDLYRTQHQEFPPECREVDYEKR
                     LRAAYPIHPEVFDRLYNDWSTLAKFQRTRGVLRLMAAVIHSLWEKGDRNPMILPANIP
                     IDDPRVQFELTRYLPDNWVPVIEKDVDGPNSLPHRLDGELPNLGKFSASRRTARTIYL
                     GSAPTATAANRGIEDRRVKLGCVMPGETPAVFGDALRRLAASATYLYQDGARYWYSTQ
                     PTVTKLAEDRAEQLRRDPDAVARELDRRLREDLRKKGDFSRVHPMPRSVQDVPDDLDA
                     RLVVLGIDHAHSKEADSPALAAARAILESRGNAPRLYRNTLVFLAVDRARLQDLDEAV
                     RRFLAWESIVADKDTLDLSPHQVKQASMQKTSADGAVTARIPEAYEWLLVPVQNSPQS
                     AVEWQVFRLSGQEPLAERASRKLRNDELLVSRFAGTRLRMELDRVPLWRGDHVEIRQL
                     VDDFARYNYLPRLVGPNVLIGAACDGVGTLTWVMDSFAYADSYDADAGRYRGLRSGQT
                     MSAEDCEHGLLVKPDVAMRQIEAETPAPVPRAVAGAAGGVRPDATPGGEGEPPAPPEQ
                     PVQVVLKRYHGTVSLDPERVGRDASQIADEIIAHLVGLVGAKVRVTLDIEAEIASGAP
                     DSVVRVVTENSRTLRFTSHGFERE"
     gene            complement(4793..7708)
                     /locus_tag="PLM74_02770"
     CDS             complement(4793..7708)
                     /locus_tag="PLM74_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013458839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1156 domain-containing protein"
                     /protein_id="HON42041.1"
                     /translation="MSIIMETGYKKKLIEVALPLEAINRASAREKSIRHGHPSTLHLW
                     WARRPLAAARAVIFAQMVDDPSAHPDLFPTEKAQEKERRRLFGIIEELVQWENTTNEE
                     VLERARAAIWQSWRATCAENVDHARAKELFDRNKLPAFHDPFAGGGSLPLEAQRLGLE
                     SYASDLNPVAVLINKAMIEIPPKFAGLPPMNPKSREENRMMPRQWKGAEGLAEDVRYY
                     GQWMRDEAEKRIGHLYPKVEITAEMAEERPDLVPLIGRKLTVIAWIWARTVKSPNPAF
                     AHVDVPLASTFMLSIKSGKEAYVEPVIEGDGYRFTVKVGKPRDPDAAKNGTKLGRGAS
                     FRCVMSGSPISSDYVYAEARTGHVGERMMAVVAEGDNGRVYLPPADEMESIARQARPE
                     WKPDVLMPENPRWFSPPLYGLRSFGDVFTPRQLVALTTFADLVQEARQLARLDAQAAG
                     LPGDSAQAGEVGSQAAAYADAVAAYLAFGVDKLSDRHSTLTRWDQTPTASGIINSFSR
                     QALPMTWDYAEGNPFSEASGNFVGGIGLIAKVVMHSLPAQFTGAASVADATVQILSSG
                     KIVSTDPPYYDNIGYADLSDFFYVWLRRSLRHVVPDLFTTLAVPKTEELVATPYRHGS
                     KDKANAFFLDGMTRAMRRLAGQAHPAFPVTVYYAFKQSESQTGEGTASTGWETFLGAT
                     IRAGFAITGTWPIRTELGNRILGQGTNTLASSIVLVCRKRPDDAPTATRREFITALRT
                     ELPKAIAHLQRSNIAPVDLAQAAIGPGMAVYTRYSEVLDAEGDALTVREALALINQTL
                     DEVLSEQEGEFDADTRWALAWFEQYGFSEGEYGVAETLSKAKDTTVEGMVRAGILVSR
                     GGKVRLLRPAELPEAWDPAKDTRYTVWETVHHLIRVLETKSESAAVELVRKLGARAQV
                     ARDLGYRLYTMCERKKRAQEALSYNGLVQSWPELARLALGDSAPDEVMATLFGEGEG"
     gene            complement(7722..11306)
                     /locus_tag="PLM74_02775"
     CDS             complement(7722..11306)
                     /locus_tag="PLM74_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HON42042.1"
                     /translation="MSRLEELLPNAAVRGVRPDCLVTVVGARWHGSDAVELTYKDPEG
                     HVANILLYRDDEPRLEVVEKGRPWSFDGDGGLFRLVSEALRIRLAHLFDPVMAVHTSL
                     IEPLPHQITAVYETMLPRQPLRFLLADDPGAGKTIMAGLLIRELIARGDLQRCMIVCP
                     GSLAEQWQDELYRRFHLSFEILTNDKLEASRTGNWFSENNLVIARLDKLSRDEDVQQK
                     LRAPGCQWDLIVVDEAHKMSATFFAGEVKFTKRYRLGQLLSTLTRHFLLMTATPHNGK
                     DADFQLFMALLDGDRFEGRYRDGAHTVDVSDIMRRMVKERLLKFDGKPLFPERIAYTV
                     PYQLSDAEARLYEEVTKYVREEFNRADSLENDKRAGTVGFALTILQRRLASSPEAIYQ
                     SLRRRRERLERRLRELRLLRRGAALAAAALAPDGPVLDPDDVEDLEDAPETEVEEVEE
                     QVLDQATAARTIDELEAEIGVLGHLESLAREVRRSGDDRKWRELASLLEEIFTPAAMA
                     NRLAEDTPPYGAGEISRPAPSPREKLVIFSEHRDTLVYLAERIRTLLGRPEAVVCIHG
                     GMGREERMKAQEFFKSDPEVRVLVATDAAGEGINLQRAHLMVNYDLPWNPNRLEQRFG
                     RIHRIGQTEVCHLWNLVAQETREGDVYQTLFRKLEEARTALGGQVFDVLGKVQFDGKP
                     LRDLLIAAIRYGELPEVREQLNRVMTEALDHERLRDLLDERALAVEAMDVSRVQRVRE
                     DMERAQARRLQPHYIESFFMEAFQRLGGVVRQREPRRYEVTHVPAQVRSRDRLIGVGE
                     PVLPRYERIVFEKSLISQQGRPPAAFVCPGHPLLDATLDLVLERHRDLLRRGTVLVDE
                     NDTGDMPRVLFYLEHALQDGAHTRTGERRVISKRMLYVELDAAGSARHVYYAPYLDYR
                     PLKPDEPDVEAVLRRPECAWISPDLEQSALGHAIACVVPEHLREVRIYRLSLIEKTYA
                     AVKDRLTKEINYWDYRASVLEEDERAGKPNARLNSREARRRADELHARLQRRLEQLNL
                     EGQISALPPVVLGGLLIVPVGLLAKMSGTGQAAPVSTVDTQEAAARARAIVMDVERGL
                     GYEPVDRETEKVGYDIESRVPGTGRLRFIEVKGRASGSATLTVTKNEILYSLNKPEDY
                     ILAIVEFVDGDQHRVHYVRTPFQREPDFGVTSVNYDFAELLARAEEPR"
     gene            complement(11747..>12303)
                     /locus_tag="PLM74_02780"
     CDS             complement(11747..>12303)
                     /locus_tag="PLM74_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HON42043.1"
                     /translation="QAPGLAVVFAILFGISGTMGMINMYYILGVIGKKYNSMRYVQLS
                     ILLIGICGGISGVVLGDLITRINTFGVSLIASVVSAAVVLIFLVFAPAFASVYYEDEW
                     AKDSKEMDIDNEQLHLFRKYKLSNREIEVCKLLLQGYTMRQIAAILSLAYPTVNTYCT
                     SIYRKLNINSRTELLLVFKDYALR"
BASE COUNT         2277 a         4101 c         3487 g         2438 t
ORIGIN      
        1 cgccggggtg cgcctatcgg ataggctgtc gcgggcgaag ccccgggata actcatgccc
       61 tagcgccaca agtcgccgaa acagtcggca gctactatta tctgctctcc ggaggcaacg
      121 tcggcgactc cggagatgcc catcaccatc gtcggcggag ctatcgcaga gtatgttccg
      181 gtccccaggt aattctgcgc gccggccccc catgcccaca cagacccatc ggccctcact
      241 gccagaccgt tgcgatcgcc ggcagtaacg gcaacgacct cgtcaaggcc ttctaccctt
      301 aagggatcgc ggtcccattt ggtgccaccg cccagttggc catggatgtt cacgccccat
      361 gcccacacag tgccgtcgtc ctttagggcc atgctatggt cagaaccggc cgcgatggag
      421 gtgactccat ggagcccaac ggactcagca tctcctacgt aacggttcag ccagtatacc
      481 gaatcacccc atggcaatgc tagctgccaa aacccccact gccatacagt gccatcagtc
      541 ttcaacgcca agctgcgata tccgctggcg gcaacagact caaccctgtc catgccggcc
      601 ttcatgacgg gcgccggtgt cgtggtcggc gcgccatgcc ctatctgata tgtgctgttg
      661 tctccccatt cccatacagt gccgtcatcc cttacagcta agctgtgaca ttctcctgcg
      721 gctatagccc gaaccccgct caggccggcc acgtgagcag gcacatcaca gcgatccgaa
      781 tacatcccat tccccagttg gccgcggtcg ttggctcccc atgcccacac ggtaccgtca
      841 gccctgagtg ccagactgtg attcgcgccg gctgcgacgg ctacgatgtc ttcaaggcca
      901 gacacccgca tcgcggcgcc atagatgtag accgacccgc gataccctca gaaatagact
      961 gtgccgtcgg ccctgagaaa tagtgcgtag ttcctgccgg caacgacctg ccgaaatcct
     1021 agactattcg acgcgctagg gacgccggcg aagctcacat tctgctcggc gccactcacc
     1081 tgccggtatc cgggttcgaa gacccagtct gcatctgccg gggcaatagt cacagatccc
     1141 ctgaggccgc tcttggtcca tgttccatca tcgttggtct ttgccgttcc gaagctgcga
     1201 ctgccactaa agctaatgta gacatctccg attccctggc ttggatcatc agcacagact
     1261 atccgccgcg agactgtata cgatattccc acgggcagcc cgcatccagc catcaagacg
     1321 cagagtgcga ttagcagcaa accgaatgcc cttctcatcg agcgcacctc caagcagtac
     1381 agcaactcaa ggttcatacc atgcccctgg ctggcctacc gccgttgagt tcgccagcca
     1441 agctcgctgt ttattcccgc tcaaacccat ggctggtgaa tctcagcgtc cggctattct
     1501 ctgttactac gcgaacgacg ctatcagggg cgcccgacgc gatctcggcc tcgatatcaa
     1561 gggttactct caccttcgcc ccgactagcc ctacaagatg tgcgatgatt tcgtcagcta
     1621 tctggctggc atctcggccc acgcgctccg gatcaaggga gactgttcca tggtatcgct
     1681 tgagcacgac ctgcacgggc tgctcaggcg gcgcgggcgg ctcaccctcc ccgcccggcg
     1741 tcgcgtctgg gcgtactccc cctgccgcgc cagcgactgc ccgcggcacg ggcgccggcg
     1801 tctccgcctc gatctgcctc atcgccacat caggcttcac cagcagccca tgttcgcagt
     1861 cctcagccga catcgtctgc cccgagcgca gcccgcggta tcgccccgca tcagcatcat
     1921 agctatcggc gtaggcgaag ctgtccatga cccaagtgag tgtgcctacc ccatcgcagg
     1981 cggctccgat cagcacattt ggtccaacaa gcctcggcag atagttatag cgcgcgaaat
     2041 cgtccacgag ctgcctgatc tctacatgat cgccccgcca tagcggcacc cgatcaagtt
     2101 ccatgcgtag acgcgtgccc gcgaagcggc tcaccagtag ctcatcgttg cgcagcttcc
     2161 tgctggcgcg ctccgccaaa ggttcctgcc ccgaaagacg gaacacctgc cattccacgg
     2221 cagactgcgg cgaattctga accggcacta gcagccactc ataggcctcc ggaatccgtg
     2281 cagtcaccgc accgtcggcg gaagttttct gcatagaggc ctgcttcacc tgatgaggcg
     2341 agaggtcgag ggtatccttg tcggcaacga tagactccca cgccaggaaa cgtcttacag
     2401 cctcgtccag atcctgaagg cgtgctctgt cgaccgccag gaataccagc gtgttgcggt
     2461 ataggcgcgg cgcgttgcct cgcgattcca gaatagccct ggccgcagcg agcgcggggc
     2521 tgtccgcttc tttgctgtgg gcatggtcga tcccgagcac aaccagcctg gcatcgaggt
     2581 catccggcac atcctgcaca gatcgcggca tgggatgcac ccggctgaag tcgcctttct
     2641 tgcgcaggtc ttcacggagc cgccgatcca gctctctcgc gacagcatca ggatcgcgtc
     2701 tgagctgctc ggcccggtct tcagccagct tggttacggt gggctgagta gaataccagt
     2761 agcgggcgcc atcctggtac aggtacgttg ccgaggcagc cagccggcgc agtgcatctc
     2821 cgaacaccgc gggagtctcg cccggcatga cgcagcccag cttcactcgc ctatcctcta
     2881 taccccggtt ggcagcagtg gccgtcggag ccgatccgag gtagatcgta cgagcagtgc
     2941 ggcgggaggc cgagaacttg cccaggttcg gcagctcgcc atctagacgg tgcggcaggg
     3001 agtttggccc gtcgacatcc ttctcgatca ctggcaccca gttatcgggg aggtagcgcg
     3061 tcagctcgaa ttgaacccga gggtcatcga taggaatgtt cgccggcaat atcatcgggt
     3121 ttcggtcgcc cttctcccat aggctgtgga tcacagcggc catgaggcgt agaacacccc
     3181 tggtgcgctg gaatttcgcc agcgtcgacc agtcgttgta gagccggtcg aacacctcag
     3241 gatgaatcgg ataggcagcc cttagccttt tctcgtaatc tacctcgcgg cactcaggcg
     3301 ggaactcctg gtgctgcgtc cggtacaggt catagaacgc gcgggctacc gtatctcttc
     3361 cgacgaactg ctcctgagac accagcggct caaacaggcg ccgccgcacg atctcgaagc
     3421 cctcctctgc gctggcaggg cgccacgacg actccactcg ccccaccacg tttcgcagcc
     3481 gattaagcgc ctctcgcccg cgatgccctc ccacctcggc atcatccgcc tgcgcatggg
     3541 gcgaagctcc agtgtcggat gcaggaagac tgatcacaag caggcactgc ctcgccagct
     3601 tcgcggactc cgtaagcgcc tgggcgaagg agaactgcgt ctcgaagctg ccggccggca
     3661 gatcgctctg gtcgtgaagc tgccgggcgt aggccaccca ctcgtctatc aatatcagac
     3721 agggcccgta cttgttgaac agctcccgca gcatgtcgcc ggggctcgtc gcgttctcgt
     3781 catcggcccg tactcgctcg aaagcttccc tgcccccgag ctgccatgcc agctctcccc
     3841 agagtgttct gacgacggtg ccgtcgggct tcgtaaccgg gttgccgggc gaaagcttgt
     3901 tccccaccag caccacgcgc ctggccgtcg gcagcttgtc cacccccgcc tcacgcagca
     3961 cgtcgtcgac ccccagcagg tcaccggagg gcgtgccaga catcaagtgg taaagcgcca
     4021 gcatagcgtg ggtcttgccg ccaccgaagt tcgtctggag ctgcaccaca ggatcgccgc
     4081 ccgtgcccgc caggcgctgc acgccccgca ccagcaagtg cttgaggctc tccgtgaggt
     4141 aggtgcgcct gtagaactcc tccgggtctc ggtactcatc gcctccctcg cccaggtgca
     4201 cctgccacag gtccgctgcg aactccgcct gctgatagtg cccgctggcc acatccgggt
     4261 gcggggtagc gacctcgcgc cacggcctca gacttcccat cgcagcactc tgaacagccg
     4321 ttccggagct cttgcgctgt tcggtgcgca tctgctcctc gaagcgcacg cgcagcaggt
     4381 tcatcttaat cctgtcgaga tcagcggcct gcggcgcgga gacggcagtc agaagtcggc
     4441 tggccgaatc aagggcacga taggcatcat cgctggagaa ccggtcctga tgggcccatt
     4501 tgttcctgac ctcccgcagc tcgctcacta ggctccgctc ggcatggccc agggtgttgc
     4561 ggaacacttc gttccactgg tcccacatga gtcgcaatag caccgcgcaa tccagatgcg
     4621 gctggtcatc gtcgccggtc cagttgacat cattaggcca agaggcagtt gcctgcgtga
     4681 tccagtactt gccgtaccgg gcccttagct cgcgctcgac gaacggcgcc agcccctcct
     4741 tgagcagatc cagcgcctca ccgacgcgtt cgtgattggt gacggccatc gtctacccct
     4801 caccttcccc aaacagtgtg gccattactt catcgggcgc gctatcgccc aatgcgagac
     4861 gagccagctc cggccagctc tgcaccagcc cgttatagga taaggcctcc tgcgcccgct
     4921 tcttcctctc gcacatggtg tataggcggt agcccaggtc ccgcgccacc tgcgcacggg
     4981 cgccgagctt cctgactagt tccaccgctg cgctctcgct cttggtttcc agcaccctaa
     5041 tgagatggtg cactgtctcc cacaccgtgt agcgtgtatc cttcgccgga tcccacgcct
     5101 caggcagctc tgcaggacgc agcaggcgca cctttccgcc cctggagacg agaatccccg
     5161 cccggaccat cccctccacc gtcgtgtcct tggccttcga gagggtctcc gccacgccgt
     5221 attcgccctc agagaagccg tactgctcaa accacgccag cgcccatcgg gtgtcggcgt
     5281 cgaactcgcc ctcctgctcc gacagcactt catccagggt ctgattgatc agagccagag
     5341 cctcgcgtac cgtcaacgcg tcgccctcgg catcgagcac ctcggagtac ctggtgtaga
     5401 ccgccatgcc cgggccgatc gccgcctgag ccaagtcgac cggggcaatg ttgctgcgct
     5461 gcaagtgcgc aatggctttg ggcagttcag tcctgagcgc ggtgatgaac tcgcgacgcg
     5521 tggcggttgg cgcatcgtcg gggcgcttgc ggcagacgag gacgatgcta gaagcaagcg
     5581 tattggtccc ctggcccaga atgcgattgc ccagctccgt tcgaatgggc cacgtgcctg
     5641 taatggcaaa gccagcacgt atcgtcgcac ccaagaaggt ctcccagccg gtactggcgg
     5701 tgccctcgcc agtctggctt tcagattgct tgaacgcata atagaccgta acagggaagg
     5761 ccgggtgggc ttggcccgca aggcgacgca tggcccgcgt catcccgtca aggaagaacg
     5821 cgttcgcctt gtctttgcta ccgtgacggt acggtgtggc taccagttcc tcggtcttgg
     5881 gcacagccaa agtggtgaag aggtcgggaa cgacgtgcct gagcgagcgg cgcagccata
     5941 catagaagaa atccgacaag tcggcgtagc cgatgttgtc gtagtatggg ggatcggtag
     6001 atacgatctt gccgctgcta agaatctgta ccgtagcatc ggcgacagat gcggcgcctg
     6061 tgaactgagc aggtaacgaa tgcattacta ccttggctat caaaccgatg ccacccacga
     6121 aattcccgga tgcttcgctg aaagggttcc cttcagcgta gtcccatgtc attggtagag
     6181 cctgtcggct aaatgagttt atgattccgc tagctgtcgg agtctggtcc catcttgtga
     6241 gggttgagtg acgatccgat agcttgtcca ccccaaacgc aaggtacgcc gctaccgcgt
     6301 ctgcgtatgc ggcagcctgc gatcctactt cgccggcctg cgcggaatcg ccaggcaggc
     6361 cagccgcctg agcgtcaagc cgcgctagtt ggcgtgcttc ctgcaccagg tcagcgaaag
     6421 tagtaagtgc tacgagctgg cgcggagtga agacatcgcc gaatgatctg agtccataca
     6481 gaggcggcga gaaccaccgg gggttctccg gcatgaggac atctggcttc cactccggtc
     6541 tcgcctgacg tgcaatgctc tccatctcat cggcgggcgg caggtagact cgcccgttgt
     6601 caccctccgc gacgacggcc atcatccgct cgcccacatg tcccgtcctc gcctcggcat
     6661 agacatagtc gctggatatc ggcgacccgg acatcacgca ccggaagctg gcaccccgcc
     6721 ccagcttggt gccgtttttc gctgcgtcag gatccctcgg cttccccacc ttcaccgtga
     6781 accggtaccc atcgccctct atcaccggct cgacataggc ttcctttcct gacttgatgg
     6841 aaagcataaa ggtagacgca agcggcacat cgacatgggc gaacgccggg ttggggcttt
     6901 ttactgtccg cgcccagatc catgcgatca ctgtgagctt gcgaccaatg aggggcacaa
     6961 ggtccgggcg ctcctccgcc atttcggctg taatctccac cttgggatat agatgaccga
     7021 tacgcttctc cgcctcatcc cgcatccact ggccgtagta gcgcacgtct tcggcgaggc
     7081 cctccgcgcc cttccactgg cgcggcatca tccggttctc ctcccgcgac tttggattca
     7141 tcggcggcag tccggcgaac ttcgggggga tctctatcat ggccttgttg ataagaaccg
     7201 ccaccgggtt cagatcgctc gcgtagcttt cgagccctag ccgctgcgcc tccaggggca
     7261 gggagccacc gccggcgaag gggtcgtgga aagcgggaag cttgttccgg tcgaacagct
     7321 cttttgcccg cgcatggtcg acattctccg cgcacgtggc gcgccaactc tgccagatcg
     7381 ctgcccgtgc gcgctccaac acctcttcgt ttgtggtgtt ctcccactga acaagctcct
     7441 caatgatgcc gaatagccga cgcctctcct tttcctgcgc cttctcagtc gggaagaggt
     7501 cgggatgcgc agacggatcg tctaccatct gggcgaagat caccgcccgc gccgcagcca
     7561 acggccgccg cgcccaccat aggtgcagcg tgctggggtg cccgtgacgt atcgacttct
     7621 ccctcgccga ggccctgttg atcgcctcaa gcggcaatgc cacttcaata agcttcttct
     7681 tgtagcctgt ctccatgatg atgctcatcc cactccattc actatctcgg ctcttcggcc
     7741 ctggccagca gctcagcgaa atcgtagttc acgcttgtca ccccgaagtc cggctctcgc
     7801 tggaagggag tgcgcacata gtgcacccga tgctggtcgc catcgacgaa ctccacaatg
     7861 gcaagtatgt aatcctcagg cttgttcagc gagtagagga tctcattctt cgtgaccgtc
     7921 aaggtcgccg acccgctggc ccgccccttg acctcaatga agcggagcct gccggtgccg
     7981 ggaacacgac tctcgatgtc gtacccaacc ttctcggttt ccctatcgac cggttcgtag
     8041 cccagcccgc gttcgacgtc catcacgata gcgcgagcgc gcgccgcggc ctcctgcgtg
     8101 tcaacggtgg agaccggcgc tgcctggccg gtgcctgaca tcttcgcgag caacccgacc
     8161 ggcacgatca gaagccctcc cagcaccact ggggggagag ccgatatctg cccttcgagg
     8221 ttgagctgtt cgaggcggcg ctgcagccgc gcgtggagct cgtctgcccg ccggcgggcc
     8281 tcccgcgagt ttaagcgggc gttgggcttg cctgcccgct cgtcctcctc aagtaccgac
     8341 gccctgtagt cccagtaatt gatctcctta gtcaggcgat ccttcaccgc cgcatatgtc
     8401 ttctcgatca acgaaagccg atatatgcgc acctcgcgca gatgctccgg caccacgcag
     8461 gcgatggcgt gcccgagcgc gctctgctca agatcagggg agatccatgc gcattcaggg
     8521 cgcctcagga ctgcctccac atcaggttca tccggcttca ggggcctgta gtcaaggtaa
     8581 ggcgcgtagt acacgtggcg ggcgctgccg gcggcgtcta gttccacgta gagcatgcgc
     8641 ttgctgatga cgcgccgctc acctgtgcgc gtgtgtgcgc catcctgcag tgcgtgctca
     8701 agatagaaca acacccgagg catatcgccg gtgtcgtttt cgtctaccag caccgttccg
     8761 cggcgaagca gatcccggtg gcgctcgaga accaggtcca gtgtggcgtc gagcagcgga
     8821 tggccggggc acacgaacgc cgcaggcggt cggccctgtt gggatatgag cgacttttcg
     8881 aacacgatcc gctcgtagcg cggaagcacc ggctcgccga cgccgatcag acgatcacgg
     8941 ctgcgaacct gcgccggcac gtgcgtcacc tcgtagcgcc tgggttctcg ctgcctgact
     9001 acgcccccaa ggcgctggaa cgcctccatg aagaacgatt cgatgtagtg cggctgcagg
     9061 cggcgcgcct gggcacgctc catgtcctcg cgcacgcgct gaacacggct tacgtccatc
     9121 gcctccaccg caagcgcccg ttcgtcaagg agatcgcgga ggcgctcatg gtcgagggcc
     9181 tcggtcataa cacgattgag ctgctcccga acctcgggga gctcaccata tctgatcgct
     9241 gcgatgagca gatcacgcag cggcttgccg tcgaactgta ccttccccag aacgtcgaac
     9301 acctgcccgc cgagggcggt gcgcgcttcc tcgagcttcc tgaacagcgt ctgatacaca
     9361 tctccctcgc gggtctcctg ggccaccaga ttccacagat ggcacacttc tgtttgaccg
     9421 attcggtgga tgcgtccgaa gcgctgctcc aggcggttgg ggttccatgg gaggtcgtag
     9481 ttcaccatga ggtgggcgcg ctgcaggttg atgccctcgc cggctgcgtc cgtcgcgacc
     9541 agcacacgta cctccggatc ggacttgaag aactcctggg ccttcatcct ttcctcgcgg
     9601 cccatgccgc cgtggataca caccactgcc tccgggcgcc cgagcaaggt tctgatccgc
     9661 tcggcgaggt acaccagggt atccctatgc tcggagaaga tgacgagctt ctcccggggc
     9721 gacggcgccg gacgggagat ctcgcccgcc ccatatggag gagtatcctc ggcgagacga
     9781 ttggccatgg cagccggcgt gaatatctcc tcaagcaagc tggccagctc ccgccatttg
     9841 cggtcatcac cgctgcggcg tacttcccgc gccagcgact ccaggtgccc cagaaccccg
     9901 atctcggcct ccagctcgtc tattgtgcgg gcagccgtag cctggtcgag cacctgttct
     9961 tccacttcct cgacctccgt ctccggcgca tcctctagat cctccacgtc gtcgggatca
    10021 agcacggggc cgtcgggcgc aagtgccgct gccgccaagg cagccccacg ccggagcagc
    10081 ctgagctcac gaaggcgcct ctccagccgc tcccggcgcc gcctcaaaga ctggtaaatg
    10141 gcctcgggcg aagaggccaa ccgcctctgg agaatggtca atgcgaaacc caccgtgccg
    10201 gcgcgcttgt cgttttcgag gctgtcggcc cggttgaact cctccctgac gtacttcgtg
    10261 acttcctcgt acagccgcgc ctcggcatcg gatagctgat atggcactgt gtaggcgatg
    10321 cgttcaggga acaggggctt tccgtcgaac ttcaggagcc tctccttaac catgcggcgc
    10381 attatgtccg acacgtccac ggtgtgcgcg ccgtcgcggt aacgcccttc gaaccggtcg
    10441 ccatccagga gggccatgaa tagctggaaa tctgcatcct tgccgttgtg cggtgtggcc
    10501 gtcatcagca ggaagtggcg ggtaagcgtg gagagcagtt gccccagcct gtagcgtttg
    10561 gtgaacttga cctcgcccgc gaagaatgtg gccgacatct tgtgggcctc gtcgacgact
    10621 atgagatccc actggcatcc gggcgccctc agcttctgct gcacgtcctc gtcgcgcgat
    10681 agcttgtcga gacgggctat caccaggttg ttctcgctga accagttgcc ggtgcgagag
    10741 gcctctagtt tgtcgttggt caggatctcg aagctcagat ggaagcgccg ataaagctca
    10801 tcctgccatt gctcggcgag gcttcccgga cacacgatca tgcatcgctg cagatcgccg
    10861 cgggcgatca gctccctgat gaggagaccg gccatgatgg tcttgcccgc tccggggtca
    10921 tccgcgagca gaaaacgcag cggctgccgg ggcagcatgg tctcatagac ggcggtgatc
    10981 tggtgtggaa ggggctcgat cagagacgta tgtacagcca tcaccgggtc aaacaggtgc
    11041 gcaaggcgga tgcgaagcgc ctccgacacc agtcggaaga ggccgccgtc tccatcgaag
    11101 ctccaaggcc ttcctttctc aaccacctct agccggggct cgtcatcgcg atagagcagt
    11161 atgttggcca catggccttc cgggtcctta tatgtaagct ctaccgcgtc cgaaccatgc
    11221 cacctggccc cgactacggt taccaggcag tcggggcgga cgccacgcac cgccgcattg
    11281 ggcaggagtt cctcaagtcg actcaccgtt catgccccac ttacctacaa gtcgatgccc
    11341 ggaggcgcct gagccgccgg gcccgtcatt tcgatatttc accttcttcg cggaaaaacc
    11401 tctagtctac gcgcccgcaa agggattgtc agactgcacg ccttcgatca cgctgccgcg
    11461 gaactcaagt gagtcctgtc ccctccatcc agggttcatt cgagcagcag ttgatgacag
    11521 cgaccttgcg tttctgcgcc gctgcggcga gattgagcgt cggaagatca atgaatcctc
    11581 tcgcttgatc ggcctcactc ggcggaggcg tacccggcgt ccagaactcc cgtcgacccc
    11641 ctgctggggg ttgtcggccg taggtatccg ccccaagact gtgtacttgt cgcatcgccc
    11701 cgccggccga gtaagttacg cggatcaaac gacatgcagc ccaatcctat ctcagtgcat
    11761 aatctttgaa gactagaagc agttcggtcc ggctgttaat attcaacttt cggtaaatag
    11821 aggtacagta tgtattgacc gttggatacg caagagaaag gatggctgca atctggcgca
    11881 ttgtatagcc ctgcagcagt agcttgcata cctcgatttc acggttgctc agcttgtatt
    11941 ttcggaacag atgaagttgt tcgttgtcta tgtccatctc ttttgaatcc tttgcccatt
    12001 cgtcctcgta gtacaccgat gcaaaggcag gcgcaaaaac caggaagatc agtaccactg
    12061 ccgccgaaac cactgaagca ataagggaaa caccgaatgt gttgattctg gtgatcagat
    12121 cacccaatac tactcccgat atcccaccgc aaatcccgat taggaggatg gataactgga
    12181 catagcgcat gctgttgtat tttttgccaa tgactccgag gatgtagtac atgttgatca
    12241 ttcccatcgt gccgcttatg ccgaacaata tggcaaacac aacggccaaa cccggagcct
    12301 gcg
//
LOCUS       DAOTAC010000038        12274 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_4379_length_12274_cov_4.171618, whole genome shotgun sequence.
ACCESSION   DAOTAC010000038 DAOTAC010000000
VERSION     DAOTAC010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 12274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12274
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4379_length_12274_cov_4.171618"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /locus_tag="PLM74_02785"
     CDS             complement(<1..861)
                     /locus_tag="PLM74_02785"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HON42044.1"
                     /translation="MHLKKLSLYGFKSFAGRVDLDLAPGIVGIVGPNGVGKSNISDAL
                     RWAMGEQSARLLRGSKMQDVIFAGSQSRKGLGYAEVSLVFDNSDQFLSIGFDEVTVTR
                     KVYRSGEAEYLLNGVPCRLRDIEDLFSGTGLGREAYSVVEQGKIDAILSARPDDRRSL
                     FDEASGITKYRARKSQAQRRLLEVRADQLRVSDVAAELGRQLPLLEAQAEKALRWQEL
                     TERLTQLDVDVLSYELSRMSQRLDALRAEIQSLQDQEHALLARAAECEAALEEARVRM
                     AQAESEVEALH"
     gene            complement(936..1637)
                     /gene="rnc"
                     /locus_tag="PLM74_02790"
     CDS             complement(936..1637)
                     /gene="rnc"
                     /locus_tag="PLM74_02790"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HON42045.1"
                     /translation="MSASTSLDEIQAKLGYEFSDQSLLLLSLTHTSYAAEHQAGHSES
                     NERLEYLGDAILKAVIADQLMRLLRLADEGRLSKLSAQILSGRALAEAALTLDLHRYI
                     RLGHGEETSGGRTRRRNLAGVMEAVIGAIYSDGGFGEARDFIVRVMEPIIERVISGPG
                     SDYKTALQEHVQGAGIGSLSYRTIRADGPSHRPWFTVEVLVGDRVVGSGQGPSKRAAE
                     QAAARCGMEMLRSAQ"
     gene            complement(1615..1863)
                     /gene="acpP"
                     /locus_tag="PLM74_02795"
     CDS             complement(1615..1863)
                     /gene="acpP"
                     /locus_tag="PLM74_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013756769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HON42046.1"
                     /translation="MTEAEIFETIREIVADKLGIPEEEIELDSDFIEDLGADSLYLNE
                     IVYELEEEFNVRVPDEAFEDLRTVGQAVKFVHERVHES"
     gene            complement(1856..2887)
                     /gene="plsX"
                     /locus_tag="PLM74_02800"
     CDS             complement(1856..2887)
                     /gene="plsX"
                     /locus_tag="PLM74_02800"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HON42047.1"
                     /translation="MRIAVDCMGGDLGPAEMVPGALKAAGECEANIVLVGDEKVIRQH
                     MGAVEGQTAAAHVDIVHAPSTISMDEDPLSAVRHKRDSSVVVAARLVREGSAEAMVSA
                     GSTGALVAIGALSVGRLPGVSRPVLATPVPTISGACVLLDIGANPDCTPEHLLEFATL
                     GSVYASSLLKLDRPRIGLLSNGTEPSKGNAMTVAANKLLTQSSLNFIGNVESRSVLFG
                     VADVVVTDGFTGNILLKSLEGVGSAIFDMVRQAAMENTRSKLGALLMKPALRQVKRRM
                     DYSEYGGAPLLGLAGLLVKAHGSSNRKAFANAIRVTSDLMGSGIGQKMAEACQIQKGS
                     SQTEVSRSD"
     gene            complement(2985..3167)
                     /gene="rpmF"
                     /locus_tag="PLM74_02805"
     CDS             complement(2985..3167)
                     /gene="rpmF"
                     /locus_tag="PLM74_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HON42048.1"
                     /translation="MANNPKQRFGKSRTRKRRANWKAEAPALSICPQCKKPKLSHSAC
                     PECGQYRGRTVLQTGE"
     gene            complement(3313..3735)
                     /locus_tag="PLM74_02810"
     CDS             complement(3313..3735)
                     /locus_tag="PLM74_02810"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HON42049.1"
                     /translation="MLLNLREEMAPIDLGRSLLKFVGPAEFVGEATNTGRRILVRGSA
                     RGRVTAVCDRCLEEFVMDLGAPIDESYYRVDEAPAEPDDDERIYGEDDIIDISSDIEQ
                     AFVLTLPIRIVCRDECRGLCPRCGRDLNTGECSCSVDD"
     gene            complement(3796..5013)
                     /locus_tag="PLM74_02815"
     CDS             complement(3796..5013)
                     /locus_tag="PLM74_02815"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HON42050.1"
                     /translation="MKVLVVNCGSSSLKYQLFDMDSKTCMARGNVERVGTAEAFIVHK
                     PNGGDKVRRDVAAADHEQAMKYVFECLTDPKIGVISDLREISAIGHRVVHGGEKFAGS
                     VLIDGAVMQALEECQDLAPLHNPPNIMGIEACRRLMPDLPMVGVFDTAFHQTMPREAY
                     LYGVGYDMYAKYGVRRYGFHGTSHRYVSARAAEILGRPVEELCMVTCHLGNGSSLAAV
                     KHGKSIDTSMGFTPLEGLVMGTRSGDIDPTIVSFLCEKLGRSASEVVLGYLNKNSGVL
                     GLSGGLSNDFRDLEEAAGRGHELAKLALDVFAYRVVKYIGAYAAAMGKLDVIVFTAGV
                     GENSGEMRRRILSRLSGFGISIDPAKNNVRGSEQIISTSESRVTVMVVPTNEEAMIAR
                     DTAEIVSRLGARS"
     gene            complement(5093..5977)
                     /locus_tag="PLM74_02820"
     CDS             complement(5093..5977)
                     /locus_tag="PLM74_02820"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HON42051.1"
                     /translation="MTGYARNLRLLVWALGCILLSAGICAASEVPSVVLRQGDRGEFV
                     CELQEYLAIAGYMDSAADGIFGSVTQQAVVAFQKSAGLTGDGIVGRKTWDALLSKAEA
                     ASARTYVVVAGDTLYGIARQFGVSVEAIASASGISRPESIKPGQKIVIPSSSGDASRA
                     ASSRSGAELLHWDVANRILKTRATIIDVVTGLSFEVQRRGGHNHADIEPLTSADTAVL
                     RRIYGGAWSWTRRAVIVVTGGRRIAASINGQPHGGQAITGNGCSGHICLHFLGSRTHA
                     SNSLDADHQRMVQAAVGH"
     gene            6153..7364
                     /gene="ylbJ"
                     /locus_tag="PLM74_02825"
     CDS             6153..7364
                     /gene="ylbJ"
                     /locus_tag="PLM74_02825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YlbJ"
                     /protein_id="HON42052.1"
                     /translation="MTAALASAVTIAIIALPEVSFRASLDGLRVWWDIVFPALLPFFI
                     ASDTLMGLGVVHYIGALLEPLMRPLFNVPGVGAFALAMGLASGYPIGARITARLRREG
                     LCSRVEAERLVSFTNTADPLFMVGAVAVGMFRIPALGAVLALAHYISAILVGLVLKHY
                     GRSEDRRSDTPPPHRREPLLSRAAHELYTARSQDNRSFGQLLGDSVKDSVSTLLLIGG
                     CIMVFSVVVRVLDVFRVTDMIARPIMFLLAPFGVSRDLVQALISGCFEITIGTEMASR
                     AAAPVIPRVVAASAIIAWSGLSVFAQAASMLFGTDVRMGVYFAARVLHAVLAAIVGLG
                     LACYGPWGAKLALTAMPGAHAAPGFFAIMGHSCVYLASIIGVLATATAAVSLAARIEV
                     VTFRVRPKGRH"
     gene            complement(7361..7804)
                     /locus_tag="PLM74_02830"
     CDS             complement(7361..7804)
                     /locus_tag="PLM74_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42053.1"
                     /translation="MKQSVIECLDRMERTIESSRKMLFSSSRIVDGVELLELIDRIRI
                     ALPEEIRQAEALQSERSRIAAESGRVEAGPSGQAQASGEVTARAREEAEAILAQARRE
                     AFEIRAGADEYAESTLSSLQETLDRTSFVVRKGIDELKRRKNDPD"
     gene            complement(7810..8295)
                     /gene="coaD"
                     /locus_tag="PLM74_02835"
     CDS             complement(7810..8295)
                     /gene="coaD"
                     /locus_tag="PLM74_02835"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010898740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HON42054.1"
                     /translation="MAVAICPGTFDPITCGHLDIITRASSIFDTVIVAILVNHGKRQM
                     FSTEERLEMLRTACAHLPNVEIDHFEGLLVDYVRKRGAHVVIRGLRVVSDFEFEMTMA
                     LMNRKLDPTIETMYIMASMEYSFLSSSLVKEVAALGGDIRCLVPASIAERVAARCRGE
                     A"
     gene            complement(8298..8867)
                     /gene="rsmD"
                     /locus_tag="PLM74_02840"
     CDS             complement(8298..8867)
                     /gene="rsmD"
                     /locus_tag="PLM74_02840"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HON42055.1"
                     /translation="MRVIAGRARGRPIACPKGRSTRPTSDRVRESLFAILGERVVDSK
                     VLDLFAGSGSLGIEALSRGAAMCVFVERDRGVCRVITDNLRSLDMQQQAVVVCADAVG
                     AIGQAPGAVSEHTPYDLVLADPPYVLEIAELLLSRIDTPRLLAGDGLLVVEHTSRTDW
                     PVRLGGLERIRQERYGDSRISFYHFAVEG"
     gene            complement(8869..9723)
                     /locus_tag="PLM74_02845"
     CDS             complement(8869..9723)
                     /locus_tag="PLM74_02845"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HON42056.1"
                     /translation="MTRIALVTDSSSDLTSEIYRENGITVVPLTVHFGDQSFVDVIEI
                     TSSEFFDKLKSSQVMPRTSQPSPADFVAVYERLLKSHDFVFSVHISSKMSGTVQSAQI
                     AAETVNPDLIEVIDGRAVSLVTGLMVLEAAEAIRQGKSVDDVRESIRHVMDNFGLYWT
                     LDTLEYLQKNGRIGRATAFIGGLLSIKPIMSIEEGEIAGVERVRGSNRVFPRILELMH
                     SRIGPGSPIDVIVLHADDESRGKRWLADVRAEFNCRHTWLTECGPIVGTHAGPGTIAV
                     AWVEPMQG"
     gene            complement(9736..11421)
                     /locus_tag="PLM74_02850"
     CDS             complement(9736..11421)
                     /locus_tag="PLM74_02850"
                     /inference="COORDINATES: protein motif:HMM:TIGR03599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HON42057.1"
                     /translation="MAGAKHDRAPMKKGLDGHALIDAFASGVAWLGENKSMVDSLNVF
                     PVPDGDTGTNMYLTVSAALKEAQKSRDSNVGAVADAIAIGSLMGARGNSGVIFSQLMR
                     GFAKRLHGLALASPSDVGAALSEAANVAYKAVMKPVEGTILTVARMGARAAVQAARDG
                     RDVQGVLDEAIRQGEITLSKTPDMLPTLKEAGVVDAGGKGLVVFMQGFAMGLRGETVS
                     AGAWAQAPVGAPQGPRPVQEEIKFRYCTEFIIRGDHLNPDAVRSSILSAAEGDSMLVV
                     GNENTIKVHVHTNSPGRVLDTCIAYGSLHEIDIDNMEDQHEEFESMGDLPKPRAASQA
                     PTPSVVKDVGVIAVAAGDGVERILMSLGADTIVTGGQTMNPSIQDIADAVQAVRAREV
                     IVLPNNGNVVLTAGKASEVPDLSSIKVHVVPSKTIPQGLAALLAMSPSASVGENIEKM
                     SEAIGKVKTGEITYAVRDSAANGLTIREHDIIGLFDGDLKAVGQDISEVARTLFSQMH
                     SSDDEIATILYGAGVDGDDAEALASSLQEQYPEVEFEVQYGGQPLYYYLISVE"
     gene            complement(11421..11798)
                     /locus_tag="PLM74_02855"
     CDS             complement(11421..11798)
                     /locus_tag="PLM74_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HON42058.1"
                     /translation="MGRAMNTELGQITIADDVIAATAGMAATECYGLVGMASRTITDG
                     IAELLGRDNIARGVEVDVEGDDVKINLYIIVEYGTRITEVANNVMDKVKYVLETTTGL
                     RVSEVNVTVHGVRVGAGGKERHK"
     gene            11967..12158
                     /gene="rpmB"
                     /locus_tag="PLM74_02860"
     CDS             11967..12158
                     /gene="rpmB"
                     /locus_tag="PLM74_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HON42059.1"
                     /translation="MPKMCAVCGKSPSSGNNVSHSELKTRRRWYPNLQKVRAMVDGSP
                     RRITVCTRCLRSGKVTRAI"
BASE COUNT         2458 a         3972 c         3249 g         2595 t
ORIGIN      
        1 atgaagagcc tctacctccg attccgcctg cgccatgcgg actctggctt cctccagcgc
       61 ggcttcgcac tctgcggccc tggcaaggag cgcgtgttcc tggtcctgaa gcgactggat
      121 ttccgcccgc aacgcgtcaa ggcgctgcga catgcgtgac aactcgtagg agagcacgtc
      181 cacatctaac tgtgtaaggc gctccgtgag ctcctgccag cgcaatgcct tctcggcctg
      241 tgcctcaagc agaggcaatt gccttcccag ctcggccgcg acgtcgctca ctcggagctg
      301 gtcggcgcgg acctccagca gccttcgctg cgcctgactc ttgcgggcgc ggtatttcgt
      361 aattcccgac gcttcgtcga acaaagaccg cctgtcgtcg ggtcgcgcag acaaaatggc
      421 gtctatcttg ccctgctcga caacggaata ggcctcgcgt cctaagcctg tgccggaaaa
      481 gaggtcctca atgtcacgaa gcctgcacgg cacgccgttg agcaggtatt cagcctcgcc
      541 ggaccgatac accttgcggg tgacagtcac ttcgtcgaat ccgatgctga ggaactggtc
      601 cgagttatca aataccagcg acacctcagc atagcccagg ccctttcggc tctggctccc
      661 cgcgaagatc acgtcctgca tcttggatcc gcgtagtagg cgggcgctct gctctcccat
      721 cgcccaccta agagcgtcgg atatgttgct cttccccacg ccgttgggac ccacgatgcc
      781 cacgatcccg ggagcaaggt ccaggtcgac gcggcctgcg aaagacttga acccgtacag
      841 agagagcttc ttaaggtgca aatctcgccc tccaataaag taggccggaa ctggccggcc
      901 cttgtagatc cgatgttgat ccgattcttc ctcgtctatt gggcagatcg cagcatctcc
      961 attccgcagc gtgcggcagc ctgctcagcg gctctcttgc tcggcccttg cccgcttcct
     1021 accacgcggt cgccgaccag aacctccacg gtgaaccagg gcctatgaga cggcccatct
     1081 gcacggatgg tcctatacga caaggacccg atgccggcgc cctgaacatg ctcctgcaga
     1141 gcggtcttgt agtcggatcc cgggcccgat atcacacgct cgatgatagg ctccatcacc
     1201 cggactatga agtcgcgcgc ctcgccgaaa ccgccgtcgc tgtagatcgc gccgatcacc
     1261 gcttccataa cccctgccag atttcgcctg cgagtacggc cgccggaggt ctcctcgcca
     1321 tggcccagcc tgatgtagcg gtgaagatcg agcgtgagcg cagcttcggc cagagcgcgc
     1381 ccgctcagga tctgggcaga cagcttagaa agcctgccct catcggccag ccggagcagg
     1441 cgcatcagct ggtctgctat gaccgccttc aagatggcgt cgccaaggta ttcgaggcgc
     1501 tcgttggatt cgctgtgccc cgcctgatgc tccgctgcat acgaagtgtg cgtaagcgaa
     1561 agaagcagca gagactggtc gctgaactcg tagcccagct ttgcctggat ctcgtcaaga
     1621 ctcgtggacg cgctcatgca caaacttgac tgcctgaccc actgtacgca ggtcttcaaa
     1681 ggcctcgtcg ggaacgcgca cgttgaactc ctcttcgagt tcgtaaacta tttcgttcag
     1741 gtacagagaa tcggcgccaa ggtcctcgat gaaatccgag tccaactcga tttcttcctc
     1801 gggtataccc agtttgtcgg ctacgatctc ccgaatggtt tcgaatatct cagcttcagt
     1861 cactgcggga cacctccgtt tggctagacc ccttttggat ttgacaggct tcggccatct
     1921 tctgcccgat gccggagccc ataagatcag acgtgaccct gatggcgttt gcaaacgcct
     1981 tccgattcga actgccatga gcctttacca gtaaccccgc gaggcccaga agaggcgcgc
     2041 ctccatactc tgagtagtcc atacgccgct tgacctgcct cagggcgggt ttcatcaaca
     2101 acgcgcctaa tttcgaccgg gtgttctcca tggcggcctg ccgcaccatg tcgaaaattg
     2161 cgctcccgac gccttccaac gatttgagca ggatgttgcc tgtgaaccca tctgtcacaa
     2221 caacgtccgc caccccgaaa aggactgagc ggctctccac attgccgatg aagttcagag
     2281 acgactgagt caggagcttg ttggcggcca ccgtcatagc gttgcccttc gacggctcgg
     2341 taccattaga caacaggcca attcgcggtc ggtcaagctt gagaagggag gatgcgtaga
     2401 ccgatccaag agtcgcgaac tccagcaggt gctcaggcgt gcagtcggga ttggcgccta
     2461 tgtccagcag aacacaggct ccgctgattg tgggaaccgg cgttgctaga acaggcctgc
     2521 tcactccggg gagcctgccg acagacagcg cgcctatggc gacgagcgcc ccggtgctgc
     2581 cggcgctcac catagcctcc gccgatccct cacgcaccag gcgcgcggcg accacgacag
     2641 atgagtcgcg tttgtgccgc actgccgaaa gcggatcctc atccatggat atcgtcgacg
     2701 gcgcgtgcac gatatctacg tgggcagcag cggtttggcc ctcgaccgcg cccatgtgct
     2761 gccttatgac cttctcgtcg ccaaccagca ctatgttggc ttcgcattcc ccggcggcct
     2821 ttagggcgcc cggcaccatc tcagcggggc cgagatcgcc gcccatgcag tcaactgcta
     2881 tgcgcatgcc cgatcgcccc tttccgcctc gaaataggga gaagcgggtt cagccgaaca
     2941 atccatgtcc gaatgaatcc gctcaacatc acgtgacacc actcctactc gccggtctgc
     3001 aaaacggtac gaccgcggta ctgcccgcac tcagggcaag cgctgtgcga cagcttgggt
     3061 ttcttgcatt gcggacaaat ggagagagct ggagcctcgg ccttccagtt cgcgcggcgc
     3121 ttgcgcgtgc gcgattttcc gaatctctgc tttgggttat tagccatgct atgggtccaa
     3181 tcgaggccta tacggaccaa agccccgacg aaacctctcc acctcctatt ttgaaaagtc
     3241 aagcgtcgtt attctactac gatttgagcg acggggcaag cagcagccta cgtaatcagg
     3301 ccagccttcc cattagtcat cgaccgaaca cgagcattcc cccgtgttca ggtcgcggcc
     3361 gcaccttggg cacagcccgc ggcactcatc cctgcacaca atccttattg gcagcgtgag
     3421 cacgaatgcc tgctctatgt ctgacgatat atcgatgatg tcatcctcgc cgtagatgcg
     3481 ctcatcatcg tcaggctcgg caggcgcctc gtcgacacgg tagtatgatt cgtcgatcgg
     3541 ggcccccaag tccatcacga actcctcaag gcatctgtcg cacacggccg tcacccgtcc
     3601 tctggcggac cctcggacca gtattcggcg gcccgtattg gtcgcctccc ccacgaattc
     3661 cgccggccct acgaacttaa ggagcgagcg ccccaagtcg atcggggcca tctcctcgcg
     3721 gaggttcagg agcattgtgg cccccttttc cgttctcaaa gactgcgcgc taaagcgcat
     3781 agcgcatcac ctctgctaag atcgggctcc cagacggctc acgatttccg ctgtgtcgcg
     3841 agctatcatg gcctcttcat tcgtgggcac gaccatgaca gtcacgcggg attcggaagt
     3901 cgatatgatc tgctcggagc ctctcacatt attcttcgca gggtctatgc tgattccgaa
     3961 cccggaaagc ctagagagaa tcctcctgcg catttcgccc gagttctccc cgactccggc
     4021 cgtgaagacg atgacatcaa gcttccccat ggccgcagca tacgctccga tgtacttcac
     4081 cactctgtaa gcgaacacgt ccagcgccag cttggcaagt tcatggccgc gacccgccgc
     4141 ttcctcgaga tcgcggaagt cgttcgatag acctccagat agcccaagca ctccagagtt
     4201 cttgttcaga tagcccagaa caacctcgct cgcgctccgg cctagcttct cgcagagaaa
     4261 ggagacaatt gtcggatcga tatcgcccga ccgggttccc atgaccagcc cttcaagagg
     4321 ggtaaagccc atgctggtat cgatagactt gccgtgcttc acagctgcaa ggcttgatcc
     4381 gttgcccagg tggcacgtga ccatacatag ctcctcgacc ggccgcccca gaatctcggc
     4441 cgcacgcgct gatacgtagc gatgagatgt gccgtggaaa ccgtacctcc ttacgccgta
     4501 tttcgcatac atgtcgtaac ccacgccgta caggtacgcc tccctgggca tggtctgatg
     4561 gaaggctgtg tcgaacactc ccaccatcgg cagatccggc atgagccttc tgcaagcctc
     4621 gatgcccatg atgttgggtg gattgtgcag aggagcaagg tcctggcatt cctccagcgc
     4681 ctgcatcact gctccgtcaa tcagcacgga tcctgcgaac ttctcgccgc catgcacaac
     4741 gcgatgccct atggcagata tctcccgaag atccgagatc acgccgattt tcgggtcggt
     4801 caggcactcg aaaacgtatt tcatcgcctg ttcatgatct gccgcggcca cgtcccgcct
     4861 gaccttgtcg cctccattgg gcttgtgaac aatgaatgcc tcggctgtgc cgactctctc
     4921 cacgtttccc cgcgccatgc aggtcttgct atccatgtcg aacagctgat acttgagtga
     4981 cgaactacca cagttgacta ccagaacctt cattcgaacc cacaacctcc ttgacgttga
     5041 aaaaagaaaa catggcatgc gccatgcttc gtccgagaac atgacgactc ccctagtgac
     5101 cgaccgccgc ctgcaccatg cgctgatgat ctgcgtccag cgagttgctg gcatgtgtgc
     5161 ggctacccag aaaatgcagg cagatgtggc cgctacagcc gttcccagtg atcgcctggc
     5221 ctccatgagg ttggccatta atggacgccg cgattcgtcg cccacccgtc accactatca
     5281 ccgcgcgcct cgtccaactc catgctcccc cgtaaatccg cctcaacacg gcggtatccg
     5341 cgctagtgag cggctcgatg tcagcatgat tgtgcccgcc gcgccgctgt acctcgaagg
     5401 agaggcctgt gacaacatcg atgatggtcg cccgggtttt gaggatccta tttgcaacat
     5461 cccagtgtag gagttccgct ccgctacgag acgatgccgc cctggacgca tccccagaag
     5521 acgacgggat cacgatcttc tggcccggct ttatcgattc cggacgggag atgccggagg
     5581 ctgatgcgat ggcctctaca gagaccccga actgcctggc aatcccgtac agggtgtcgc
     5641 ccgccactac cacgtatgtg cgggcagagg cagcctcggc cttgctcaaa agcgcatccc
     5701 aggtcttcct gcccacgata ccatctccag tgagcccggc ggacttttga aaagcgacta
     5761 ccgcctgctg ggtgacggat ccgaagatcc catcggcggc agaatccata taccctgcaa
     5821 tggccagata ctcctgcaat tcacatacga attcgcctcg gtcgccctgc ctcagcacta
     5881 cgctggggac ttccgatgca gcacatattc ccgcggaaag caggatgcac ccaagggccc
     5941 acaccaagag gcgcaggtta cgagcgtacc cggtcaccat agtcgccctc cttcaccttg
     6001 tatcctggcc tttattctac tgaacgctgc aaattccttc agcatacccg cgggccaagc
     6061 caaatataga ttctcaggac ggcctcccac aacgaaagga cgtgtatcct cagatgaaaa
     6121 ggcgcataga gcccagaaga agaggtgtct acgtgacggc tgccctggct tcggcggtca
     6181 cgattgccat catcgcactc cccgaagtgt cgttcagagc ctcgctggac ggactgcgcg
     6241 tatggtggga catagtcttc cctgcactgc tcccgttctt catcgcctca gatacgctaa
     6301 tgggcctggg agtagtccac tacatcgggg ctttgctcga accgctgatg cgtcctctgt
     6361 tcaacgtgcc gggtgtagga gctttcgccc tggccatggg gcttgcatcg ggttacccca
     6421 tcggcgcccg tatcaccgct cgcctacgca gagagggact gtgctcgcgt gttgaggcgg
     6481 aaaggctcgt atcgttcacg aacaccgcgg atccactatt catggtgggt gccgtcgcag
     6541 tcggcatgtt cagaataccc gcgctcggcg ccgtgctggc tctggctcac tacatatcag
     6601 ctatactcgt cggcctcgtt cttaagcact acggtcgaag cgaagacagg cgctccgata
     6661 ccccgccccc tcaccgccgt gagcccctgc tatcacgagc cgcccatgag ctgtacacgg
     6721 cgcgaagtca ggacaatcgt tcgttcggcc agctcttggg cgattcagtt aaagactccg
     6781 tcagcaccct gctacttatc ggcggatgca taatggtttt ctccgtggta gtccgtgtgc
     6841 tcgacgtgtt ccgtgtcacc gacatgatcg cgagacccat catgttctta ctcgccccgt
     6901 ttggggtctc ccgcgacctg gtgcaggcac tcataagcgg gtgcttcgag ataaccatag
     6961 ggacggagat ggcgagtcgg gcggctgctc cagtgatccc cagagtcgtc gcagcgagcg
     7021 ccatcatagc ctggagcggg ctgtcagtgt tcgcccaggc ggcaagcatg ctcttcggca
     7081 cagatgtccg catgggggta tatttcgccg cgagggtact ccacgccgtg ctggcagcca
     7141 ttgtcgggct aggacttgcg tgctatggac cgtggggggc taagctcgca ctaacagcaa
     7201 tgcccggcgc gcatgccgca ccgggcttct ttgccatcat gggccattcc tgcgtctacc
     7261 tggcctcgat catcggcgta ctcgcgaccg cgacggcggc ggtgtcgctc gcagccagga
     7321 tagaggtagt gacgttccgg gtgaggccca agggccgtca ctaatcagga tcgttcttcc
     7381 ttcgcttcag ttcgtcgatg cctttgcgaa ctacaaacga cgtccgatcc agcgtctcct
     7441 ggagagacga cagcgtagac tcggcatact cgtccgcgcc ggctcgtatc tcaaatgcct
     7501 cacgcctcgc ctgggccaaa atcgcctcgg cttcctccct ggcgcgtgcc gtgacctctc
     7561 cgctcgcttg agcctgaccc gacgggcccg cttcgacgcg accggactcc gccgcgatgc
     7621 gggatctctc agactggagc gcttccgcct gccttatctc ctcgggcaag gcgatgcgaa
     7681 tcctgtctat cagttcgagc agctctacac cgtctacgat gcgacttgac gaaaagagca
     7741 ttttccggct ggattcgata gtccgctcca tcctgtcgag gcactcgata actgactgtt
     7801 tcaatatcat cacgcctccc ccctacaccg cgcggcaaca cgctccgcta tcgatgcagg
     7861 caccaaacac cgtatgtcgc cacctagggc cgcgacctcc ttcaccaggc tagaactgag
     7921 aaacgagtat tccatggagg ccattatgta cattgtctcg atggtggggt ctaacttcct
     7981 gttcatcaga gccatggtca tctcgaactc gaagtccgac actacgcgaa gacccctgat
     8041 cactacgtgg gcgccgcgct ttctgacata gtcgacgagc aacccttcga aatggtcgat
     8101 ctccacatta ggcagatggg cgcatgcagt ccttagcatc tccagtcttt cctcagtgga
     8161 gaacatctgc cgcttgccgt ggttaacaag tatggccacg atcacggtgt cgaagattga
     8221 cgaggccctc gtaatgatgt cgagatggcc gcaggttatg gggtcaaaag tgccggggca
     8281 tatggcgaca gccaatctca tccctccacc gcaaagtggt agaaactgat cctgctatct
     8341 ccgtaccgtt cctgccttat tctctccaaa cctccaaggc ggaccggcca gtcggtgcgt
     8401 gaagtatgtt ctacgacgag cagcccatct cctgcgagca atcgaggagt atcgatccgc
     8461 gaaaggagca gttcggcgat ttccagcacg tatgggggat ccgcgagaac cagatcgtac
     8521 ggcgtgtgct ccgacaccgc gcccggggcc tgtccaatcg ctccgaccgc atctgcgcac
     8581 acgaccacag cctgctgctg catgtccaga gatctgaggt tatctgtgat cacgcggcaa
     8641 acaccacggt cccgctcgac aaagacgcac atcgccgccc ctctcgacag cgcctctatt
     8701 cccaggctcc cgctgccggc gaacaggtcc aacaccttcg aatcgacaac tcgttcgccg
     8761 aggatggcaa agagcgactc gcgaacacga tcggaggtgg gacgagtcga tctgcccttt
     8821 gggcaggcta tcggacgccc acgcgcccgc ccggctataa ctcgcatgtc agccctgcat
     8881 cggctcaacc caggccactg caattgtgcc cgggccggca tgtgtgccga ctataggccc
     8941 gcactcagtc agccatgtgt gccgacagtt gaactctgcc ctaacgtcag caagccatcg
     9001 cttcccacgc gactcatcgt cggcgtgcag tactatgaca tcaatggggg aacctgggcc
     9061 aattcgacta tgcatcagct ccaggatccg cgggaacacc cggtttgacc ctcggaccct
     9121 ctcaaccccg gctatctcgc cttcttcaat ggacatgatg ggcttaatgg agagcaaccc
     9181 tccgatgaac gctgtggccc gccctatgcg gccgttcttc tgcaaatact ccagtgtatc
     9241 cagcgtccag tagagcccga agttgtccat gacatgtcgt atgctttcac gcacatcatc
     9301 cacactcttg ccctgtcgaa tcgcctctgc agcctccagc accatcaggc cggtcacgag
     9361 cgaaacggcc cgaccgtcga tgacctcgat cagatccggg ttcacggtct ctgcagcaat
     9421 ctgagcgctc tgcacggtgc cgctcatctt ggacgatatg tgcaccgaga atacgaaatc
     9481 gtgcgacttg agcaagcgct cgtacacggc cacaaagtcg gccggcgaag gctggctggt
     9541 tctgggcatg acttgcgagc tctttagttt atcgaagaac tcagagcttg tgatctcaat
     9601 gacgtcgacg aagctttgat cgccaaagtg cactgtaagg ggcacgactg tgatgccgtt
     9661 ctcccggtat atctcgctgg taaggtcgga actgctgtcg gtaacaagcg cgattcgcgt
     9721 catattccat cctcctcact caaccgagat caggtagtaa tagagcggct gccccccgta
     9781 ctgaacctcg aactccacct cgggatattg ctcttgcagg ctggacgcca gagcctctgc
     9841 gtcatcacca tcgacgcccg ccccgtacag aatcgtggct atctcatcgt cggacgagtg
     9901 catctgagag aaaagcgtgc gggcaacctc ggaaatatcc tgtcctactg ccttgaggtc
     9961 accatcgaac agcccgatga tgtcatgctc tcggatagtc agaccattgg cggcagagtc
    10021 acgcaccgcg taagtgatct cgcccgtctt caccttccct atggcttccg acatcttctc
    10081 gatgttctcg cccactgaag cagatggaga catggccaga agagcggcca gaccctgcgg
    10141 aatcgtcttg ctgggaacta cgtgcacctt gatactcgaa aggtcgggaa cctctgaggc
    10201 ctttcccgca gtcaacacaa cgttgccgtt gttcgggagc acaatgacct ccctggcgcg
    10261 cacggcctgc acagcgtcgg caatatcctg aatgctcgga ttcattgtct ggccgccggt
    10321 cactatggta tcggcgccca ggctcataag gatcctctcc actccatcac cggcagccac
    10381 cgcaatgacc ccgacatcct tgaccacaga cggggtcggt gcctggctcg ccgcccgggg
    10441 ttttggcaaa tcccccatcg actcgaactc ttcgtgctga tcttccatgt tgtcgatgtc
    10501 gatctcgtgt agcgaaccat acgcaatgca cgtatccagc acccgtccgg gcgaattcgt
    10561 gtgaacatgc accttgatcg tgttctcgtt tcccacgacc agcattgagt cgccctccgc
    10621 agccgaaagg attgagctcc ttactgcatc tgggttcagg tgatcgcctc tgatgataaa
    10681 ctctgtacag tacctgaact tgatctcttc ctgcaccggc ctgggcccct gaggggcgcc
    10741 cacgggggcc tgcgcccacg ctcctgccga cactgtctcc ccgcgcaacc ccatggcaaa
    10801 tccctgcatg aagacgacaa gacctttccc gccggcatcc accacccctg cctccttaag
    10861 agttggcagc atatcagggg tcttgctcag ggtgatctcg ccctgccgta tggcctcgtc
    10921 gagcacgccc tgcacatctc gaccatcgcg ggccgcctga accgcggcgc gcgcacccat
    10981 ccgcgccacc gtcagtatgg ttccctccac aggcttcatc acagccttgt acgctacatt
    11041 ggccgcttct gacaaagccg ctccaacgtc agacggggag gctaaggcaa gcccgtggag
    11101 ccgcttggcg aaaccccgca ttagctgcga gaatatgacc cctgagtttc cgcgtgcgcc
    11161 catcagcgac ccaatggcta tagcatcggc aactgcgccc acattggaat cgcgagattt
    11221 ctgcgcttcc ttgagcgcag cagagaccgt taggtacata ttagttccgg tatcaccgtc
    11281 gggaacaggg aatacgttga gtgagtcaac catgctcttg ttctcaccca gccaggcaac
    11341 tccggacgca aaggcatcga tgagagcgtg cccatcaagt cccttcttca taggcgcccg
    11401 gtcgtgcttc gctccagcca ttatttgtgc cgttcctttc cgcccgcacc cacccgtacg
    11461 ccgtggacag tcacattgac ttccgaaaca cgaaggcccg tggtagtctc cagaacgtac
    11521 ttgaccttat ccatgacgtt attcgctact tcggtaattc gggtgccgta ttcgacaatg
    11581 atatacaggt tgatctttac atcgtcgcct tctacatcga cttctactcc gcgggcaatg
    11641 ttgtcgcgtc ccaagagctc agcaatgcca tcggtaatcg tacgcgatgc cataccgacc
    11701 agcccgtagc attcagtagc ggccattccg gctgtagccg ctatcacatc gtctgctatc
    11761 gttatctgcc cgagctccgt attcatagct ctgcccattg cgaaacctcc aatgcgaaca
    11821 tacgagacaa tggaatcgtg ggccaaacat ttttcatcta ttctatagat gtatgagcta
    11881 ttccttcttg gtgtgccctt gcgtagtcta cccggctgtg ataacattta agttgtgcgt
    11941 gtcaggcgct gcaaggagga attcgcatgc ccaagatgtg tgcggtctgt ggcaagtccc
    12001 cgtcatccgg gaacaacgtc agccactccg agcttaagac gaggcgccgt tggtatccga
    12061 acctccagaa agtcagggct atggtcgatg ggagtcctcg acggataacc gtgtgcacca
    12121 gatgccttcg ttcgggaaag gtgacccggg cgatctgaca tacggcctcg atctgaacag
    12181 ctttttcaag tgacgccccc tttatcgggg gcgtttcaca ttgtcaagcc tctctctata
    12241 agggccttgc tccgcagttg gcgcacgatg ccgt
//
LOCUS       DAOTAC010000039        12065 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_4480_length_12065_cov_4.821815, whole genome shotgun sequence.
ACCESSION   DAOTAC010000039 DAOTAC010000000
VERSION     DAOTAC010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 12065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12065
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4480_length_12065_cov_4.821815"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..401
                     /locus_tag="PLM74_02865"
     CDS             159..401
                     /locus_tag="PLM74_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HON42060.1"
                     /translation="MATKDYVFHLGDVVRMRKPHPCGCLTWEVLRVGADFRIKCQGCG
                     RVVMLPRPQFVKGVREIVSTSAGGDSHAEPGDRGPA"
     gene            373..1452
                     /gene="ychF"
                     /locus_tag="PLM74_02870"
     CDS             373..1452
                     /gene="ychF"
                     /locus_tag="PLM74_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HON42061.1"
                     /translation="MSLGIVGLPNVGKSTLFNALTRGRAQVANYPFTTIDPNVGVVEV
                     PDDRLAQVARLAGAEKVTPTVVQFVDIAGLVKGASRGEGLGNKFLSHIREVDAVAHVV
                     RCFTDPNVAHVDGALDPVRDMDIVETELILADLDTVGRRIAKTEPMLKTGDKRYRFEL
                     DTLMELKGLLESGRPAREMDLSFECLQLVESLFLLTMKPTMIIANLPEECADRLDEAA
                     AEISLREGVPVIPVCAELERQLGELEPEDARAYLASLGIERSALDSVVTAGYRLLDLI
                     TFFTIKGAETRAWTVRLGTSAPKAAGKVHTDMERGFIRAEVIQWDLLLQEGSFGAARD
                     HGRVRTEGKDYVVQDGDVILFRFNV"
     gene            1582..1869
                     /gene="rpsF"
                     /locus_tag="PLM74_02875"
     CDS             1582..1869
                     /gene="rpsF"
                     /locus_tag="PLM74_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012996264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HON42062.1"
                     /translation="MHAYESMYILSPTFGDEEIDGLISKFEELITSNGGEVTKTDRMG
                     RRRLAYVIGDHTEGYYVILYFNATADIVAELERVYKITDGVVRYLIIRKDK"
     gene            1910..2335
                     /locus_tag="PLM74_02880"
     CDS             1910..2335
                     /locus_tag="PLM74_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014345612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HON42063.1"
                     /translation="MNRIVLVGRLTRDPELRVTQNGVSVARFTVAVDRPFASQSGERG
                     TDFIDVVVWRKQAETVGTYMKKGRLVAVEGRLQVRTYQAADGQKRRAWEVIGDRVEFL
                     DRGPDSAARVPAVEEPPFPEPGEDGEPTFGDEIDLGEPF"
     gene            2377..2607
                     /gene="rpsR"
                     /locus_tag="PLM74_02885"
     CDS             2377..2607
                     /gene="rpsR"
                     /locus_tag="PLM74_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HON42064.1"
                     /translation="MKRERGRRSRRRVCSFCVDKVEHVDYKDTARLRRYISERGKIMP
                     RRISGNCAKHQRQVTLAIKRARHVALLPFTAE"
     gene            2880..3194
                     /locus_tag="PLM74_02890"
     CDS             2880..3194
                     /locus_tag="PLM74_02890"
                     /inference="COORDINATES: protein motif:HMM:NF024057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HON42065.1"
                     /translation="MGHQRDTDIARSFKTIDWLKAEVLGSVASVYRSLASTDEDPRAS
                     DLANAIIGCYLLAKRLGISYAALDEQININIRENIQNGHEVEQWYKDFSALASHRKGR
                     SS"
     gene            3191..4150
                     /locus_tag="PLM74_02895"
     CDS             3191..4150
                     /locus_tag="PLM74_02895"
                     /inference="COORDINATES: protein motif:HMM:NF021506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HON42066.1"
                     /translation="MNSPRTERTRALTEGALLAALTIVMYVADIYTKLLLYVIPVPVA
                     ILVIRNGMRTGVLATLVAALGVGAILGPIDGLIVLVRVGFIGIALGALLARKRSWLIS
                     LAGTSAASLAAFVSDFLLASWASGLSPGAMIAKVQEVFTEAGQRTMELYQKMGVPQES
                     LGLVRQMTELMPVWLKTFLPAVLVIGSVFSASIAYAATRWILVRMKRNVEPIPPFADW
                     RIDWRWAWGLIGALVLAYAVPAVNLGFVRSLAANAVAVYVMVYSLCGIAVLWSVLGSM
                     NVPKGFRVILALFIYMTPPFNWLLPMAGLLDGWLDFRKLASRA"
     gene            4240..4698
                     /gene="rplI"
                     /locus_tag="PLM74_02900"
     CDS             4240..4698
                     /gene="rplI"
                     /locus_tag="PLM74_02900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HON42067.1"
                     /translation="MKVVLQKDVPGLGKKDTAVDVAEGYARNYLIPRGLALPASSAAL
                     KRIEVIRDAERRKEAREEAKAREDARKLKEHGVTIRAKAGEKGRLYGSITSKDVADAI
                     RAVIGVDVDRRKIELKDPIRELGEHMVTVKLFSGVSADVMITVVPTEESR"
     gene            4785..6713
                     /gene="lonC"
                     /locus_tag="PLM74_02905"
     CDS             4785..6713
                     /gene="lonC"
                     /locus_tag="PLM74_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018130443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lon family ATP-dependent protease"
                     /protein_id="HON42068.1"
                     /translation="MGSRNLADKLQGDEQLMRQVMSMFGILSDLYGPDKLVLKAGKLD
                     ALDLMRSSKIEDRILALERLVFEDPTIDKVHKPDEYEAVLSDIEDEIADTIARRRVED
                     KIEKRISDRMQQRHEDYVNEIKMQILKEDSGPDTPETLRKLEELEQLEARQLARSAME
                     IIRPASMSEIVGQDRAVKALLAKVASPFPQHVILYGPPGVGKTTAARLALEEAKKVKL
                     APFAADARFVEVDGTTLRWDPREVTNPLLGSVHDPIYQGAKRDLAEGGVPEPKLGLVT
                     DAHGGVLFIDEIGEMDPILLNKLLKVMEDKRVTFDSSYYDPADPNVPQYIKRLFEQGA
                     PADFVLIGATTRDPDDLSPALRSRTAEVFFEPLTQKHIQEIVTNAATKLGVKIDSDIP
                     AIVSDYTIEGRKATSLLVDAYGLALFRQVQSDGASAGGVTITRADLEETIRLGRLSPY
                     VHARASHTSDVGKIFGLGVAGFLGSVIEIEAVTFPAREEAKGAIRFNETAGSMAKDSV
                     FNAASVFRRITGQDMADYDVHVNVVGGGDIDGPSAGTAVLLAILSSVYSCPIRQDVAV
                     TGELSIQGKVREVGGIFEKIYGARQAGIRKVIMPAENAKDVPSDITGIEVVPVASVSE
                     VFAHVFDGDIDFRKPNEE"
     gene            6719..8083
                     /gene="dnaB"
                     /locus_tag="PLM74_02910"
     CDS             6719..8083
                     /gene="dnaB"
                     /locus_tag="PLM74_02910"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HON42069.1"
                     /translation="MASVGVAGVERVPPQSLEAEQSTIGSMLLDKEAIATAAEILVAE
                     DFYRDAHRVIFDTLIELFNRGEPADLITVTEELRTRNALERVGGASYISTLANSVPTS
                     SNCEFYAKIVKNKSILRALVAAGAEIAALGYDVSADVDNSLDKAEQLIFRIAQSGEAG
                     TVADMKTVLMSTFDRIERLYSTKGALTGLPTGFVELDNMLSGLQPSELVVVAARPSMG
                     KTALALNIAEHVGLNERKPVLIFSLEMSREQLAARMLCSQASVDGQRLRRGNLTEADW
                     PRLSNALGRLSEAPVFIDDTPSVSALEIRTRARRLKAEHGLGIVIIDYLQLVQGRARV
                     ENRQQEIAEITRSLKALARELEVPVLALAQLSRAVEATADKRPLLSHLKESGEIEQSA
                     DVVAFIYREDYYKPDIEAERRNIAEIIVAKQRNGPTGSFELLWQREYTRFRNLEKYAQ
                     AAPA"
     gene            8098..8922
                     /locus_tag="PLM74_02915"
     CDS             8098..8922
                     /locus_tag="PLM74_02915"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HON42070.1"
                     /translation="MRRRALLAGVGAVVFVMLMMSASSQPGLASAWPAPVAPVAPAAL
                     NSQSRPLVLLVHGWLATSLDLLAIRLRLTRDGFDCDAIDFSYLGVLDSISDYAGELAW
                     KIEAEYADRRNIAVVAHSMGGLVTRYYLRNLRTTDQVKTVITIATPHHGTFGGMILPP
                     RSTPTSEMLPDSPFLEELNSVSETVPGVDFYAIWTPTDGVVIPPENSIMCGARNYKVS
                     GLGVTHLGLLMSDAAYAIILDVLHGRKPPATGPQTRDHDEAKRNPLPWLKLRDETQ"
     gene            9221..11806
                     /gene="mgtA"
                     /locus_tag="PLM74_02920"
     CDS             9221..11806
                     /gene="mgtA"
                     /locus_tag="PLM74_02920"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HON42071.1"
                     /translation="MFMSLSTEMAARPGRSTLLNLPLPELMERLAASSDGLSTEEAEK
                     RIERYGRNELAHRRVRPPVVSFLMLLAGPLNLVLIAAGVVSAFFGEMSGAIIIGIMVL
                     ASTTLEFVQEYTSERAAQKLARQVAVTATVLRDGVTTEIHMSEVVPGDVIYLSVGDIV
                     PADARVVAAKDFFVHQAALTGESQPVEKVLFSGSRPHNTPLPDMDHVVFFGTNVVSGT
                     ATALAVETNGRTEFGRITKSLTAERPETEFQRGIREFTRLLMRVIGVLVVFIFVLNAS
                     LHRGVLNSLLFAVAVSVGITPELLPMIITINLSTGARTMARNKVIVKRLQSIQNLGSM
                     DILCTDKTGTLTEGQLAVHSHVDAEGNESERTMELATVNSVFQASLRNPMDAAIARHA
                     SIDLSSYTKIDEIPFDFTRRRMSVIVAKAGRRMLITKGAPEGVLEVCTRVEFGADGVD
                     VREMTPELKAAALDEFVRLSSDGYRAVAVAYRTIGNGREVYSISDESNMTFVGYVSFI
                     DPPKESTREALEQAEALGVEIKILTGDNELVTTRICERVGLKVKSVLMGQDVDSMSDD
                     ELARRAEQATVCARLSPDQKDRVISALKRSGHVVGYMGDGINDAPSLRAADVGVSVSN
                     AVDVAREAADIILLQEGLDVLETGIVEGRKTFANTLKYIMMGTSSNFGNMFSVPAALF
                     LVPFLPMQPVQILLNNLLYDFAQVTIPTDKVDDDYIAEPKRWDIEFIKKFMIVFGPIS
                     SLYDVLTYLVMLYVFHADAPLFQTGWFVESLATQTLVIHVIRSRHSILRSRASLPLTL
                     SSLAVVAAGLAIPYTPIGRFFGFVPLPTAFFAVLLAMTAAYLAMVNAVKKVFYRKAGV
                     WGAGI"
     gene            11925..12000
                     /locus_tag="PLM74_02925"
     tRNA            11925..12000
                     /locus_tag="PLM74_02925"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11958..11960,aa:Lys,seq:ttt)
BASE COUNT         2552 a         3250 c         3860 g         2403 t
ORIGIN      
        1 gacagcgcag gcgaggggac taggccgagg tccgaccgag catcctgaga gaacacacgg
       61 ggctcggggt gggggcggcg gctggagctc cggggccggc cagcacggca gccggtctgg
      121 cggatggcga ggaatgagtc ggatctgggg gcagagacat ggcgactaag gattatgtct
      181 tccacctggg cgatgtggtt cggatgagaa aaccgcatcc ctgtggatgt cttacatggg
      241 aagtgctgag ggtgggcgcg gactttcgca tcaagtgcca gggctgcgga cgtgtggtca
      301 tgttgcctcg accgcaattc gtgaagggtg ttcgggagat cgtgtcaacc tctgcaggag
      361 gcgattcgca tgctgagcct ggggatcgtg ggcctgccta acgtgggcaa atcgacgcta
      421 ttcaacgcgc tgacgcgcgg tcgggcccag gttgccaatt acccgtttac tacgatcgat
      481 cccaatgtgg gggtagtcga agtgccggac gaccggctcg cgcaggtggc gcggctcgcc
      541 ggagccgaaa aggtgacccc gactgtggtc cagttcgtgg acatagcagg acttgtcaaa
      601 ggggcgagcc gcggggaagg gctgggaaac aagtttctgt ctcatataag ggaagtggat
      661 gcagtggccc acgtggttcg ttgcttcacc gatcccaacg tggcccacgt agacggcgcg
      721 ctcgatcccg ttcgcgacat ggatatcgta gagacagagc ttatcctggc tgatctggac
      781 acggtgggta gacggatagc caagacggag ccaatgctga aaacaggcga caagaggtac
      841 agattcgagc tggacactct aatggaactg aagggattgc tggaatccgg gcgaccggcg
      901 cgcgagatgg atctttcttt cgaatgtctc cagttggtag agagtctctt cctactaacg
      961 atgaagccga cgatgatcat agccaacttg ccggaggagt gtgcagaccg gcttgatgag
     1021 gccgcagcgg aaatctcgtt gcgtgagggc gtgcctgtta ttcccgtctg cgcggagttg
     1081 gagcgacagc tcggcgaatt ggaaccggag gacgcccggg cttacctcgc ttctcttggc
     1141 attgaacgtt ctgcgttgga tagcgttgtt acagccgggt atcgtctgct cgatctcata
     1201 acgtttttca ccataaaggg agctgaaacg cgcgcatgga ctgtgcgact gggcacttcc
     1261 gcgccgaagg cggcagggaa ggtccacacc gacatggagc gtggtttcat acgcgccgag
     1321 gtcattcagt gggatctact gctgcaggaa ggttccttcg gcgccgctcg tgatcatggg
     1381 cgcgtcagga ccgagggcaa ggactatgtc gttcaggacg gcgatgtcat tctattcagg
     1441 ttcaacgtgt gatcagggcg ttgttctgga aatatcaccc gtacggtggg ttgcgcctcc
     1501 ccgggcctaa tgttataatt catctcgcaa tccctgctcc gcgcgtgacg cggggccgaa
     1561 gtccgagggg aggtgaagtc agtgcacgcc tacgagtcaa tgtatatcct cagtcccacg
     1621 tttggcgatg aggagatcga cggtttgatc tccaagttcg aggagctgat cacgtcaaac
     1681 ggcggagaag tcaccaagac cgaccgcatg ggtaggcgca ggctggcgta tgtgataggg
     1741 gatcataccg aagggtatta tgttatcctc tatttcaacg caactgccga tatcgtggcc
     1801 gagctggagc gcgtctacaa gatcaccgac ggcgtggtac gctacctgat catccgcaag
     1861 gacaaatgac tccttgccgg gcctaaattt gaggcagggt ggtgttgtca tgaacaggat
     1921 cgtactggtg ggtcgcttga cacgcgatcc ggaactccgc gtgacgcaga acggcgtaag
     1981 cgtagccagg ttcactgttg cagtcgacag gcctttcgcc agtcagtcag gcgaacgcgg
     2041 gactgacttc atcgacgtgg tggtctggcg caagcaggca gagacggtgg gcacctatat
     2101 gaagaagggc cgtcttgtcg ctgtggaggg aagacttcaa gtccgtacct accaggctgc
     2161 cgatgggcag aagcgtaggg cgtgggaagt aataggcgac agggtggagt ttctggaccg
     2221 agggcctgac tctgcagcgc gcgttccagc ggtggaggaa ccgcctttcc ccgaaccggg
     2281 tgaagacggc gagcccacgt tcggagacga aatcgatctc ggcgagcctt tctaggcacc
     2341 acgcgccgct gcttcagcta ttggggggac atcacattga agcgtgaaag aggacgcaga
     2401 tccagaaggc gagtctgctc cttctgtgtg gacaaggtcg aacatgtcga ctacaaggat
     2461 actgccaggc tccgcagata tatctcggag aggggcaaga taatgcctcg ccgcatatcc
     2521 ggcaactgcg cgaagcatca gcgccaggtt accctggcga tcaaacgcgc ccgtcacgtt
     2581 gcgctgctcc cgttcactgc ggagtagcag gcgattgtcg ctgggctgca ggcgggcagc
     2641 tggggcctgc caggcttcag tatcagccga atccgaaaac tctttggagc ctccgggtcc
     2701 aaagagtttt ctcgcacata tcccttgcca cgtgcgctcg gcaggattta gcgtcgagca
     2761 tcgagaacga acggcatcga gaaccgatag ctacgaggtt gcgtgcgtca ggtgtgcatc
     2821 cgtgcgtcag ccacgaaact aggcatgggc ttcctacacg tgggcaggga ggaattggga
     2881 tgggacacca acgtgacacc gacattgcgc gtagtttcaa gacaattgac tggctcaagg
     2941 ccgaggtgct ggggagtgta gcttcagtgt accgctccct ggcaagcacc gatgaggatc
     3001 cgcgtgcgtc ggatcttgcc aatgcaatca tcggctgcta tctgttggcc aagcggctgg
     3061 gcattagcta tgccgccctt gacgagcaga ttaacatcaa cattcgggag aacatacaga
     3121 acggacacga agtcgagcag tggtacaagg acttcagtgc actcgcgagc cacaggaagg
     3181 ggcgttccag ttgaactcac cgcgcactga gcgaaccagg gcgctcactg aaggggcgct
     3241 tctggctgcc cttacgatag tgatgtacgt agccgacata tacaccaaac tgctgctgta
     3301 cgtaatacct gttcccgtag cgatcctggt tatcaggaac ggcatgcgta ccggcgtcct
     3361 tgccactctg gtagcggctc tgggcgtggg cgcgatcctc ggtccgatag atggcctgat
     3421 cgtgctggtc cgtgtgggtt tcatagggat cgccctcggt gcgctgcttg ccaggaaacg
     3481 ctcgtggctc atctctctcg cgggcacttc agcagccagt ctagctgcct ttgtgagcga
     3541 cttcctgctg gcgtcatggg cgtcggggct ttcgccgggc gccatgatag ccaaggttca
     3601 ggaggtgttc accgaggccg ggcagcgtac tatggaactg taccagaaga tgggcgtgcc
     3661 gcaggagtcc ctagggttgg tcaggcagat gactgagttg atgccggttt ggctcaagac
     3721 gtttctccca gccgtgctgg tgattggctc agtcttctcg gcgtcgatag catatgccgc
     3781 cacgcgctgg attctcgtga gaatgaagcg caatgtcgag cccattccgc ctttcgctga
     3841 ctggagaatc gactggcgct gggcatgggg gttgataggc gcgctagtgc tggcctatgc
     3901 tgtgcctgcg gtgaatcttg gtttcgtgcg ctccctggcg gcaaatgcgg tcgcagtcta
     3961 tgtcatggtc tattccttat gcggaatcgc agtgctctgg tcggtgctag ggtctatgaa
     4021 tgttcccaag gggttcaggg tgatccttgc cctgttcatc tacatgactc cgccctttaa
     4081 ctggcttctg cccatggccg gtctgctcga tggctggctc gacttcagga aacttgcgtc
     4141 gcgagcataa tgccctgtcc ggcagagatg catcggcaag atacgcgcag catacggaga
     4201 ggaggtagcg ggtgagtcac ggttcgcccg cgggcacaca tgaaagtagt ccttcaaaaa
     4261 gacgttccgg ggcttggcaa gaaggatacg gcagtagacg tggccgaagg ctatgcgcgc
     4321 aactacctaa tacccagagg actagccctt ccagcatcat cggcggcgct caagcggatc
     4381 gaggtgattc gagacgcgga gcggcgcaag gaggcacgtg aggaagctaa ggccagagaa
     4441 gacgctcgca agctaaagga gcatggcgtc acgattaggg ccaaggcagg cgagaagggc
     4501 aggttgtacg gttccatcac ctccaaggac gtagccgatg cgatccgggc ggtcatcggt
     4561 gtggatgtgg atcgccgaaa aatcgagctc aaggatccca tcagggaact tggggaacat
     4621 atggtgactg taaagctctt ctccggtgtg tcggcagatg ttatgattac tgtcgtgccg
     4681 acggaagaaa gccgctaggt cgcgtgaccg ccggcgcgct atcatagcgc atgcgctggg
     4741 ggaggaattg cgtcatgaag tcgctcgttg acaagatcat caagatgggg agcaggaacc
     4801 tggccgataa actccagggc gacgagcaac tcatgcggca ggtaatgtcc atgttcggga
     4861 tactctcaga cctgtacggc cccgacaagc tagtgctcaa ggccggaaag ctggatgcgc
     4921 tcgatctgat gcgctcatcc aagatagaag atcgcatact cgcgctcgag cgcttggttt
     4981 ttgaggatcc cactattgac aaggtgcaca agcctgatga atacgaagcc gtcctgtcag
     5041 acatagaaga cgagatagcc gacactatag cccgacggcg tgtggaagat aagatcgaga
     5101 agcggatctc ggaccgcatg cagcagaggc acgaagatta cgtgaacgag atcaagatgc
     5161 agatattgaa ggaggattca ggccccgaca cgccggagac ccttcgcaag ctggaggagc
     5221 tagagcagct cgaagcgagg cagctggcga ggtctgccat ggagatcatc cgtcctgcgt
     5281 cgatgtcgga gatcgtgggt caggaccgcg cggttaaggc gcttctggcc aaagtggcct
     5341 ccccatttcc gcagcacgtg atcttgtatg gtccgccggg agtgggaaag accacggcgg
     5401 cgcgtctggc gctggaagag gcgaaaaagg tcaagctggc cccgtttgca gccgacgcga
     5461 ggtttgtaga ggtggacggc acgacgctgc gctgggatcc gcgtgaggtc accaatccgc
     5521 tgctggggtc ggtgcacgac ccgatctacc agggggccaa gcgcgacctt gcggaaggcg
     5581 gggtgcccga gcccaagctg ggcctggtta cggacgctca cggtggtgtg ctgttcatag
     5641 acgagatagg ggagatggac ccgattctcc taaacaagct gctcaaggtc atggaagaca
     5701 agcgggttac ctttgattcg tcttactacg atccggccga ccccaatgta ccccagtaca
     5761 tcaagagact gtttgagcag ggcgcgccgg cggatttcgt cctcataggc gccacgacgc
     5821 gtgacccgga cgacctaagt ccggcgctgc gctcgcgcac ggctgaggtc tttttcgaac
     5881 ccctaactca gaagcacatc caggagatcg tgacgaatgc cgcaactaag ctgggtgtga
     5941 aaatcgactc cgacatcccg gcaatagtga gcgactatac gatcgaaggc cgaaaggcca
     6001 ccagcctgct ggtggacgct tacgggctgg cgcttttccg tcaagtgcag tctgacggcg
     6061 ccagcgccgg cggcgtgaca atcacgaggg ccgatctgga ggagaccatc aggctggggc
     6121 gattgtcgcc ctacgtgcat gcccgggcgt cgcatacctc cgacgttggc aagatattcg
     6181 gcctgggggt tgctggcttt ctgggctcgg tcatcgagat cgaggcggtt acgttccctg
     6241 cgcgggaaga ggccaagggc gcgatcaggt tcaatgagac tgccggcagc atggcgaaag
     6301 actcagtgtt caacgcggcg tctgtgttca ggcggatcac agggcaggat atggccgact
     6361 acgatgtgca cgtcaacgta gtgggcggcg gcgatatcga tgggccgtcc gcaggaactg
     6421 cagtgcttct cgctatactc agttcagtct attcctgccc catcaggcag gacgtggccg
     6481 tcacgggcga gttgtcaatc cagggcaagg tgcgcgaggt gggcggcata ttcgagaaaa
     6541 tctacggagc gaggcaagcg ggcatacgca aggtgatcat gccggccgaa aacgccaaag
     6601 acgttcccag cgacataacc ggcatagagg tggttccggt tgccagtgtc tccgaggttt
     6661 tcgcccatgt atttgacgga gacatagact tccgcaaacc aaacgaggag tgagagactt
     6721 ggcatctgta ggtgtagcag gcgttgagcg cgtgccgcct cagagtcttg aagcggagca
     6781 gtcgaccata ggttcgatgc ttctggataa agaggcgatc gcgacggctg cagagatcct
     6841 ggtcgccgag gatttctaca gagacgccca ccgagtaatc tttgacaccc tgattgagct
     6901 gttcaaccgc ggcgaaccgg ccgacctgat cactgtgacc gaggaactcc gcacacgaaa
     6961 tgcgctcgaa cgcgtgggcg gagcatcata cataagtacc ctggcgaact ctgtgcccac
     7021 atcgtccaac tgcgagttct acgcaaagat cgtgaagaac aagtccatac tgcgggcgct
     7081 ggtagctgcg ggcgccgaga tagccgcgct gggctacgac gtgtcggctg atgttgacaa
     7141 ctcgctagac aaggcagagc aactaatatt ccggatcgca cagagcggcg aggcgggcac
     7201 ggtggcagac atgaagaccg tgctcatgag cacgttcgac cggatagagc ggctctattc
     7261 caccaagggt gcgctcacag ggctgccgac agggttcgtt gaactggaca atatgctctc
     7321 cgggttgcag ccgtctgagc tggtagtagt tgccgcgagg ccgtctatgg gcaagacggc
     7381 gcttgcactt aacatagcgg agcacgtggg cctaaatgag aggaagcctg tcctcatatt
     7441 cagccttgag atgtcgcgcg agcagctggc tgccagaatg ctgtgttcac aggccagcgt
     7501 ggacgggcag cgccttcgcc gtgggaacct caccgaggca gactggccgc ggctttccaa
     7561 tgccctgggc cgtctgagcg aggcgccggt ctttatcgac gataccccca gtgtttcggc
     7621 tctggaaata cgcacccggg ctcggcgact caaagccgag cacggcctgg ggatagtcat
     7681 cattgactac ctgcagctcg ttcagggcag ggcacgcgtg gagaatcggc agcaggagat
     7741 cgccgagatc acccgttcgc tcaaggcctt agcccgcgag ctagaggtgc cggtattggc
     7801 cctggcgcag ctttccagag ctgtggaggc cactgccgac aagcgaccgc tcctatcgca
     7861 cctgaaggag agcggagaga tcgaacagtc ggcggacgta gtggcattca tctaccgcga
     7921 ggattactac aagccggaca tcgaggctga acgtcggaac atagccgaga tcatcgtggc
     7981 caaacagcgc aacggaccta cgggcagctt cgagctccta tggcagcggg agtacacccg
     8041 attccgcaac ctggagaagt acgcgcaggc tgcgccggca taggatggtg tgtggccatg
     8101 cgtaggcgag cgcttcttgc gggggtcggt gcagtcgtgt tcgtcatgct gatgatgagt
     8161 gcgtcgagcc agcccggcct cgcgagtgct tggccagctc cggtggcccc ggtggccccc
     8221 gcggcgctca atagtcaatc gcgtcctttg gtgttgcttg tccatggatg gttagccacg
     8281 tccctcgacc tgctggcgat aaggttgcgc cttacccggg acgggttcga ctgtgatgcg
     8341 attgacttct cgtacttggg cgtcctagac tccatatcgg actacgccgg cgagctcgca
     8401 tggaagatcg aggcagagta cgcagaccgc aggaacatcg cagttgtagc gcatagcatg
     8461 ggtgggctcg tcacgcggta ctacctgaga aatctgcgca cgacagacca ggtcaagacg
     8521 gtcatcacca ttgccactcc tcaccacggc acgttcggcg ggatgatctt gcctccaagg
     8581 tccactccaa ctagcgagat gctgccggat agccccttct tggaagaact gaacagtgtg
     8641 tcggagactg ttcctggcgt ggacttctac gccatatgga cgccaaccga tggcgtggtg
     8701 attccaccgg agaactctat catgtgcggg gctcgaaact acaaggtatc ggggctcggc
     8761 gtcacccacc tgggattgct catgagcgat gccgcctatg caataatcct ggacgtgcta
     8821 cacgggcgga aaccgccggc gaccggacct cagacgcgcg accatgatga ggccaagaga
     8881 aatccgctgc cgtggctgaa gttgcgcgat gagacgcaat gagccaagga attgctgggc
     8941 cgggcggtct ggctgaggca ttgctgcctt tgggatagcg cgcatgggac gcggttgaca
     9001 tcaatcgacg acaagcatat tctgactatg gtgtttgccg gacctgagaa atagttggcg
     9061 aggtgagccg ttaatgagta ccgacggcgc aggcagtaga cctatcagca gcatactatc
     9121 tatgatcgtt cgggaaggag cggcgcccac gcctgtgagg ctgcgctaac cttcgtgggc
     9181 atgttcgcgc ctccccgaaa cgacggggag gcgcattcac atgttcatgt cgctgtccac
     9241 cgagatggcc gcaaggccag gacgtagcac cctgctcaac ctgccgcttc cggagctaat
     9301 ggagcggttg gccgcatcga gcgacggcct ttccactgaa gaagccgaaa agcgtattga
     9361 gcgatatggc aggaacgagt tggctcacag aagagtgaga ccgcctgttg tatcgtttct
     9421 gatgttgctg gcgggccccc tcaacctggt gttgattgca gcgggggtgg tatcggcgtt
     9481 cttcggcgaa atgtctggag ccatcatcat cggcatcatg gtgttggcca gcactactct
     9541 cgagttcgtc caggagtata catcagagcg agctgcgcag aagcttgcca ggcaagtggc
     9601 cgtcacagcg actgtcctac gcgacggggt gacgactgag atccacatga gcgaggttgt
     9661 ccctggagat gtgatctatc tgagcgttgg cgacatcgtt ccggccgatg ccagagtggt
     9721 cgctgccaag gatttcttcg tccatcaggc ggcgctcacg ggcgaatcgc agccggtcga
     9781 gaaggtcctt ttctcgggat cgcgcccgca caacactccg ctacctgaca tggaccatgt
     9841 ggtcttcttc ggaaccaacg tggtaagcgg aacagcaact gcgctggcgg tggagaccaa
     9901 cggccgcact gaattcggcc gcattaccaa gagcctaaca gctgagcggc cagagaccga
     9961 attccagcgc gggatccgtg aattcacccg gttgctgatg cgagtgatcg gtgtgctcgt
    10021 ggtgttcatc ttcgtcctca atgcctcgct ccaccgtgga gtgctcaatt ccttgctctt
    10081 cgcagtagcg gtctcggtgg gcatcacccc cgaactgctg ccgatgataa tcaccatcaa
    10141 cctgtccacc ggggcacgga cgatggccag gaacaaagtg atcgtgaagc gcctgcagtc
    10201 catacagaac ctggggagca tggacattct gtgtaccgac aagactggga cgctcaccga
    10261 aggacagcta gccgtgcact cgcacgtgga tgccgagggc aacgagtccg aacgcacgat
    10321 ggagctggcg acggtgaact ccgtctttca ggcaagcctc agaaacccga tggatgcagc
    10381 catagccagg cacgcttcga tcgatctatc gagctacacg aagatagacg agatcccgtt
    10441 cgacttcacg cgccgacgga tgtcagtcat tgtagccaag gccggtcgca ggatgctgat
    10501 aaccaagggg gcgcccgagg gtgtgctgga ggtgtgcacc cgggttgagt tcggggcgga
    10561 cggggttgat gtgcgcgaga tgaccccgga gcttaaagct gctgctctgg acgaattcgt
    10621 aagactatca tcggacggtt accgcgcggt ggcagtcgcg tacaggacca taggcaacgg
    10681 ccgcgaggtc tattcgattt cggacgagtc gaacatgacg ttcgtggggt atgtatcgtt
    10741 catcgatcct cccaaggaga gcacgcgcga ggctctagag caggcagagg ctctgggagt
    10801 cgagatcaag atactcacag gggacaatga actggtcact acgcgaatat gtgaacgcgt
    10861 ggggctcaaa gtgaagagcg tgctcatggg gcaggatgtg gactccatga gcgatgacga
    10921 gctcgcccgc cgcgcagaac aggcgactgt gtgcgctcgg ctcagtccgg accagaagga
    10981 cagagtcata tctgccttaa agaggtcagg gcatgttgta gggtacatgg gcgatggtat
    11041 caacgacgcg ccgtcgctca gagcggccga tgttggagtg tcggttagca acgccgtgga
    11101 cgtagcgaga gaggcggccg acataatcct cctacaggaa ggcctggacg ttctggagac
    11161 cgggatcgtg gaaggccgga agacctttgc caacacgctt aagtacatca tgatgggcac
    11221 cagctccaat ttcgggaaca tgttcagcgt tccggcagcg ttgttcctag tgccgttcct
    11281 gcccatgcag ccggtgcaga tactcctgaa caacctgctc tacgactttg cgcaggtcac
    11341 gataccgact gacaaggtag acgacgacta catcgccgag ccaaagcgct gggacatcga
    11401 gttcatcaag aagttcatga tcgtgtttgg tccaatcagc tctctgtatg acgtgctcac
    11461 ttaccttgtg atgctatacg tgttccacgc cgacgctccg ctgttccaga ccgggtggtt
    11521 cgttgaatcc ctggccacac agaccctggt cattcacgtc attcggtcaa gacacagcat
    11581 actgagaagc agagccagcc tgcctctgac gctatcgagc ctggcagtag tggcggctgg
    11641 gctagccatc ccctatacgc caatcggccg ctttttcggg ttcgttccgc tgccgactgc
    11701 gtttttcgca gtgctactcg ccatgaccgc ggcatacctg gccatggtga acgcggtcaa
    11761 gaaggtcttc tacaggaagg ccggggtgtg gggagctgga atatgatgca tcgttaggag
    11821 gtattcgtgg gcattcgcag gcgtctcgtg gcgtcacgtg gcgtcacgtg gccttgaaac
    11881 gcccatagca gctgtggtat aatcacttgc tgaagatatt gagtgggccg ctagctcagc
    11941 cggtagagca ccggactttt aatccgggcg tcgcgggttc gaatcccgcg cggctcacca
    12001 ttattatgcc ccttttagct ggtccgatga taaatgccgc tggggactgc gcccagcggc
    12061 attcg
//
LOCUS       DAOTAC010000040        12004 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_4510_length_12004_cov_4.588752, whole genome shotgun sequence.
ACCESSION   DAOTAC010000040 DAOTAC010000000
VERSION     DAOTAC010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 12004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12004
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4510_length_12004_cov_4.588752"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1192..1749
                     /locus_tag="PLM74_02930"
     CDS             1192..1749
                     /locus_tag="PLM74_02930"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019686942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HON42072.1"
                     /translation="MPVIHGERVVLREFRKEDVAPIQAWVGNEEITRYLGFVMFPQSL
                     EETTSFVERQLVRRGTPDEGVFVIALRDDPSLEYVGAVGLHGISWRDRHCELGIVIGR
                     EDLLGKGLGTEAIRLALGFAFDFLNMHKVNLTVFEYNERGRRCYTKCGFKEEGRIREQ
                     RFYNGHYWDVIIMGITREEFAAVAR"
     gene            complement(1764..3056)
                     /locus_tag="PLM74_02935"
     CDS             complement(1764..3056)
                     /locus_tag="PLM74_02935"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HON42073.1"
                     /translation="MRLISLRKGRNATTLLNATLVVFLAAMILANIASSMENPLMPLY
                     LQSLGASVQQVGLFFTLTAIVPLVFQILGGFISDSIGRLQAIAIGSTAGLIGYIGYVL
                     APSWKWLLLAQGVASLAFCFVAPSFQAFIAEQSSEETRGRVFSVVDSVYMIVGVIGPP
                     LGGYVSKYYGYRAMFGVALVLYGSATMIRIYMARKANSGAARESSPPARDRLTFAGLK
                     HNLVSMASMVLAGGVITWLFISDGIGDVAYSIASRFEPLYYSNIIGLDNTQIGSFLSA
                     HHLVMMALLPVGGWFADRVGERAGIVLSNVAFAAGFAVFLAGKSYGHFMLVWIILGTG
                     ASFGRPAYNSLISKAVPLSMRGTAFGLFTTSLGLVSLPAPYIGGLLWNSFGPKATFVV
                     PFAASLAAAPLLWVKLSPAEIAKSVKKASEAAESPAEA"
     gene            complement(3142..3615)
                     /locus_tag="PLM74_02940"
     CDS             complement(3142..3615)
                     /locus_tag="PLM74_02940"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HON42074.1"
                     /translation="MPYCPECGAEFDAGVKFCAECGARLERVSASGQTSRVKGEPQAQ
                     QLEHVLWEGKPYLKEITTNTKYILTTQRLIIQMGLLGRREEQIDLGRVKDIRLTQGLS
                     DRMIGIGQIEIISTDPSTPSFRLMGIKDPRKVRDLIWSAVNQRRSAMGIRPRELL"
     gene            3814..4209
                     /locus_tag="PLM74_02945"
     CDS             3814..4209
                     /locus_tag="PLM74_02945"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HON42075.1"
                     /translation="MTKDGDARYWRDEAYEALRAAEVLLDNRRYVESGFFCHLATEKM
                     LKALVARRTHRTPPRMHALWRLAEIAGVEDDFNAKQVDTIADLSVFQVEGRYPTDRSA
                     LLDANPPERFKDLYDRTKEVLSCLDSLLR"
     gene            4185..4532
                     /locus_tag="PLM74_02950"
     CDS             4185..4532
                     /locus_tag="PLM74_02950"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HON42076.1"
                     /translation="MSGLPPEVAEAVGRFVRELESRIPVDRVILFGSYAHGTAVDDSD
                     IDIAVVSSAFGGNAWDDRRLLFDTMIAARLDSRLEPHPLSPRDYEAPSSWVQTEILDK
                     GIVVFARTDHSGE"
     gene            complement(4632..6137)
                     /locus_tag="PLM74_02955"
     CDS             complement(4632..6137)
                     /locus_tag="PLM74_02955"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HON42077.1"
                     /translation="MVGLKTGLIMPTDDIVEITLDTVKETVQDGDIVCVTEAVVARSQ
                     NRYVTVDELVQDLRSKLSVSEEGTLAVISPIVSRNRFVLVLSAIARAVRRGKVIVQLS
                     VPYDEVGNQVMDEDFANSRFRLKRTLGSLLEVRGNTPQMNVLIREILAALKFQELGYT
                     VTAIRKITGKGIADITLHDPEGRHLVAEVTFEDMPGTAEKVLRIAQDSDADGALVAAV
                     DLQTREISIVDAAGLLEGAAKPHVYPYSDRLALYDARDVITLGEIGERLFPHPITGID
                     YARMYAKAIEAEGARCDILYTNNPLAVFNYGHIDGIIIGAVHERESLKNLFLSFGTKT
                     PMLTVKDVGPGPWGVIGSNVSDLDAGILKLLPDNADDVCDTIRDRVREATGKDIEVLI
                     FGDGAYKDPDTGIYELADPYPSIGCSVGLRKASLRQGTKLKLLVETLFRQGRSREEIA
                     RELASRAPSRDSLGTTPRRITGILATMADLAAGSADAGTPIVLIRNFPHKG"
     gene            complement(6385..8490)
                     /locus_tag="PLM74_02960"
     CDS             complement(6385..8490)
                     /locus_tag="PLM74_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HON42078.1"
                     /translation="MASEIVQQPRIAEAWRHFVNTGEVDSSILRPEIAASWRRSSEAM
                     VDPKDALGRLPAGRRGIDDLLDDHCELIRVARPFMSSLHRFVVGSGFIVILADERGRV
                     MEIIGDEDALDRAGLVNLVPGSSWSEHHVGTNGIGTALACMAPLQVSGYEHYCERFHT
                     WTCSAAPILDERGHAIGALQMSGPSTRTHAHTLGMVVAAAEAIRAQMRINAKMRELTV
                     LNDNLNKMFLITSDGILLLDDRGMIRRANPVAEGILGAPMGDPGADAHSGADAHTDAH
                     TEGHAGGRAGPDGLYTAPVYELVNKPHYFTNMLGAGKAFDDVEMALKTPSGPVHCLVA
                     AKPVYDDHGNVNGGVVSLNPSKRITKLISRYSGSYATFRFDDILGRDEKLRRAVEVAS
                     IAAQSDSSVLICGESGTGKEMIAQAIHNHGKRACGPFVPVNCGAIPRELVGSELFGYE
                     EGAFTGAARGGRPGKFELAAGGTLFLDEIGDMPLEQQVALHRVLQDRRVIRIGGSAVT
                     QVDVRVISATNKDLLQAVAKGSFRQDLYYRLNVIQIQLPPLRERPDDIPLLFDFFMAK
                     AGRRLGSEVGSVDPRVVECLMNYDWPGNVRELQNVVERMVSIAADGRIGLQHVPEEIM
                     CHSPEDAQPAAFANIEDERRRMRRMLERREQEQLLTLMQKHGGNISRVARELGVSRNT
                     IYRKIRKAAAGESIRPTAQ"
     gene            8849..10324
                     /locus_tag="PLM74_02965"
     CDS             8849..10324
                     /locus_tag="PLM74_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HON42079.1"
                     /translation="MHKPVDRYYKLFIDGTWVDSKGGKTFDTYCPANGELLATCADAG
                     KEDVDLAVGSAWKAFDSWKNTSPVERSTILLKIADLIDANAEKLAMVETIDNGKPIRE
                     TMSVDVPLASDHFRYFAGVIRADEGSAVMIDKDHMSIILSEPIGVVGQIVPWNFPFLM
                     AAWKIAPALAAGCTVVIKPSSTTPLSTLEFAKLLQEVVPPGVVNVVTGRGSTTGEYML
                     KHPGFRKLAFTGSTEVGYDIARAAADKLIPATLELGGKSANIYFPDCPWDKAIEGVQV
                     GILFNQGQVCCAGSRVFVHEDIYDKFLAECVAAFNKVKVGLPWEEDTIMGSQVNETQL
                     KKILSYIDIGKKEGARVACGGERITDGELAKGAFMKPTILADVTNDMRVAREEIFGPV
                     VCFIKFRDEDEVVKMANDNEYGLGGAVWTRDINRALRVARAVETGRMWINDYNNLPAH
                     APFGGYKKSGIGRETHKMMLAHYTQKKNIFISTSEAKKGMY"
     assembly_gap    10478..10577
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10578..11807)
                     /locus_tag="PLM74_02970"
     CDS             complement(10578..11807)
                     /locus_tag="PLM74_02970"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HON42080.1"
                     /translation="MDVYVARQPIYDTRLNVSAYELLFRSSLNDVFSCSDSDYASLAL
                     LVNSFIAIGIDRLSNGRPVHVNFTQQLLQDGIPSLFPPDVVVVEVLENTMPSPAVLDA
                     LARLKEQGFSIALDDYIGQPHLEPFLKLVDIVKVDFLLASHAQRAAIPRSAVKPNTVF
                     LAEKVATRDEFQTAVRHGYRLFQGNFLSRPEIAKGSDIPPDALASLHLLRQVHDPSPD
                     FDTLASIISRDVSLSYRLLRVVNSAAFGLRHRVTSIKHALVIMGLSEIRRWIAVLTVT
                     GLSGEKPRALAEAAIVRARMTELLATPAGLAHRASELFLTGLLSLMDALLDRPMSEVV
                     DLLPLTEDTRTALLGEAGPFLPVLQMVTAYENAQWEQVEATAPALGIHQAFLPEAYAD
                     SLAWADELGRLEKCYAC"
BASE COUNT         2475 a         3631 c         3406 g         2392 t
ORIGIN      
        1 cagataggag tgccgggaat gaagtagtat atatggtcca ggagcatcaa ccccatggcc
       61 agcagcttga gcctgaacgc gtccagccct gtcttcatca cttgccccgc acccccatac
      121 atcggtcagc tcatctgcgc cacatctgtt ggctccatat tcgccttcac gcaccatcac
      181 acctctcgtc tccccaagcc gacattgtct ttcccggccc gcaagctcgc cctcgcacat
      241 ggcggcccgc ccgcccccac ccagccagct cgaccagctc gccaagtgca tgcaggcttt
      301 gtgagcaata gcagttggcg aaggggatag acatcgccag gctggatagt gagcggcgag
      361 acaacaccct ccccgatcat tacggtggtt gttggctgag ggcgtcatgc tgacctcgcc
      421 ggaatggcta tttagctggt cttggcatca tggctgtcgg acaaaacggc tggagctggc
      481 atatcgtcgg tttttgccat tagttttgga gatacccgca acttttgagt acatcgattt
      541 cgaagagcca cctgaccgcc tccaaatcac gaaaatcggg gggtatccga cgggtccggg
      601 cagtaaagca ggcggcgagc cgacataata ccccatatca tggcgccatt tggtaatggc
      661 gtgtgagcga tggcatgttg tgccagcgat tcgccactaa atcggcgcca tatggccccc
      721 agggctcctt ttgcggggcc gattggcccc ccggggcccc cggcccaaat cgatgtactc
      781 acaaattgcg tttttcgccg aaagtattgc cgaaaagtga ccgtatctct gggcggatac
      841 ccccgggggt atatccctga gccacagcag tcatctttcc gtcacttagc acgcccccgg
      901 atctaccccc ggacttaccc ctgggcctat ccccggatgt atcgctgggc ccatccccag
      961 atctatccct gagcccattc ctggacctac ccgcgggtct atccctggat ccctggcccc
     1021 ggtgcggccc caaccgcttc cgctatggcc ctggcgagat tgcacgtctg aatgggggta
     1081 tctgcgacgc gaaagcaaca atcgccacac gcaccccagc tcgccgggta gcatggacga
     1141 acgcgaaccg cacgtggtat gatagtccca ttattcagca agagagaggc gattccagtg
     1201 atccacggtg agcgagttgt gctgcgcgag ttccggaagg aagacgtcgc gcccattcaa
     1261 gcctgggtcg gaaatgagga aataacgagg tatctcgggt ttgtgatgtt cccccaatcg
     1321 ctggaggaga ctacatcctt cgtcgagagg cagctagtca gaaggggaac gccggatgaa
     1381 ggggtcttcg tcatcgccct ccgtgatgat cccagcctgg agtacgtagg ggctgtaggc
     1441 cttcacggca taagctggcg cgaccgtcac tgcgagctgg ggatcgtaat aggccgtgag
     1501 gacctgctgg gcaaggggtt gggcacagaa gccatacggc tagctctggg gttcgcgttc
     1561 gatttcctga acatgcacaa ggtgaacctg acggttttcg agtacaatga gcgcggtcgc
     1621 agatgctata ccaagtgcgg attcaaggag gaaggccgca tcagggagca gcggttctac
     1681 aacggacact actgggacgt catcatcatg ggtatcacgc gcgaggagtt cgccgccgtc
     1741 gcacgctagg ccgccgtcgt gcgctaggcc tcagcaggcg attccgcagc ctccgacgct
     1801 tttttcacgc tctttgctat ttcggccggg ctcagtttca cccagagcag cggcgccgcc
     1861 gccaggctgg cggcgaacgg gacaacaaac gtggctttgg ggccgaacga attccacagc
     1921 agtccgccga tgtatggggc aggcagagat acaagcccga ggctggtcgt aaagagcccg
     1981 aaggccgtcc cgcgcattga gagcggcact gctttcgata tcagcgagtt gtatgccggc
     2041 ctaccgaacg atgcccctgt gccgaggatg atccacacca gcatgaaatg cccatagctc
     2101 ttgccggcca gaaacaccgc gaatccggcc gcgaacgcaa cgttgcttag gacaatcccg
     2161 gcgcgctcgc ccacccgatc ggcgaaccat ccgccgacag gaaggagcgc catcatcacc
     2221 aggtggtgag ctgacaggaa cgatccgatt tgggtattgt cgaggccaat gatattgctg
     2281 tagtacagcg gctcgaaccg cgacgctatg ctgtaggcta catcgcctat tccgtctgag
     2341 ataaacagcc acgtgatgac gccgcccgcg agcaccatag acgccatgga gaccaggttg
     2401 tgcttgagcc ccgcaaacgt gaggcggtcg cgcgcgggag gactcgactc ccgggccgca
     2461 ccgctgttcg ctttgcgggc catatagatc cgtatcatcg tcgccgatcc atacagaacg
     2521 agcgctaccc cgaacatggc ccggtatccg tagtacttcg acacataccc cccaagcgga
     2581 gggccgatga ctcccacgat catgtaaacg gagtccacca cagagaagac ccgcccgcgc
     2641 gtctcttcag acgactgctc tgcgatgaaa gcctgaaaac tgggggccac aaagcagaag
     2701 gccagcgagg ccacgccctg cgcgaggagg agccacttcc aggacggagc cagtacgtat
     2761 cctatgtacc cgataagccc ggccgtactg cctatcgcga ttgcctgcaa tcggccaatg
     2821 gagtcagaga tgaacccgcc tagtatctga aagaccaacg gcactatggc cgtgagtgtg
     2881 aagaacagcc caacctgctg cacactggcg cccagagact gaaggtacag aggcatcagc
     2941 gggttctcca tactcgaagc tatgttggcg agtatcatgg cagctaagaa gaccactagc
     3001 gtcgcgttga gcagagtggt cgcgttccgg cctttccgca gactgatcaa cctcatctac
     3061 cttcatctct cccatgtaca tgacaacgcc cggcgacgtc cgaccggcgg cgtgcactcc
     3121 gaatcatact gagccaaacg gtcacagcag ttcgcgcggg cgaatcccca ttgcgcttcg
     3181 gcgctggttg acggcgctcc aaatgaggtc gcgcacttta cgcggatcct taatacccat
     3241 caggcggaat gatggcgtcg acggatcagt ggagataatc tcgatctgcc cgatgcctat
     3301 catccggtct gaaaggcctt gcgtaaggcg gatatccttc accctcccaa ggtctatctg
     3361 ctcttccctt cgcccaagta gccccatctg aatgatgaga cgctgggtag tcaggatgta
     3421 cttcgtattg gtcgttatct ccttcaggta gggcttcccc tcccacagca catgctccaa
     3481 ctgctgcgcc tgcggttcgc ccttaacccg cgaggtctgg cctgaggcac taaccctctc
     3541 cagccgcgca ccgcactccg cgcagaactt gacgcctgca tcgaactccg ctccgcattc
     3601 gggacaataa ggcaacaacg acacctcccc tatcgccatc ggtcctctga ccggttcaat
     3661 actatacgac tcccgttcct ttttcaaata gccccaaatg ctggccgcct gggccgacgg
     3721 atggtagtat gtacatgggc caaattgcat gggcgcatcg gcgctctggg ctcgaccgtc
     3781 tttggccggc gcgcgccgag ggaggcgata accttgacga aagacgggga cgcgcgctac
     3841 tggcgcgatg aggcttacga agcgcttcgg gctgccgagg ttctgctgga caaccgccga
     3901 tacgtggaaa gcggcttctt ctgccaccta gcgacggaga agatgctcaa ggcgctggtt
     3961 gcccggcgaa cccatcgaac gcccccgcga atgcacgcgt tgtggcgtct tgccgagata
     4021 gccggtgtgg aggacgactt caacgccaaa caggtggata ccatcgcgga cctgtcggtg
     4081 ttccaggtag agggccgcta tccgactgat cgaagcgcgt tgctggacgc caacccgccc
     4141 gagaggttca aggacctgta tgacagaaca aaggaggtcc tgtcatgtct ggactccctc
     4201 ctgaggtagc tgaggcggta ggccgatttg tccgcgagtt ggagagcaga atcccagttg
     4261 atcgggtcat tctgttcggg tcgtacgctc acggaactgc agtcgacgac agcgatatcg
     4321 atattgccgt ggtgtcttca gcattcgggg gaaatgcctg ggacgaccgg cgccttctct
     4381 ttgacacaat gatcgcagcc cgcctggact cgcgcctgga accgcaccct ctttcgcccc
     4441 gtgattatga ggcgccgtct agctgggtgc agacagagat acttgacaaa ggcatcgtcg
     4501 tcttcgcccg gaccgatcac agcggcgaat aggaaaggcc ccgccgcggc ggggcctaca
     4561 tttcacactc tgctgatatc acatgctgct catattgtgc gttcagcgtt cagcgttcag
     4621 cgtcctgcga ctcaaccctt gtgcggaaag ttccgtatca gcactatggg tgttcccgca
     4681 tcggcagaac ccgcagcgag gtcggccatg gtggccagaa tgccggtgat cctgcgcgga
     4741 gtggtgccca ggctgtcgcg ggacggcgcc cgggaggcta gttccctggc gatctcttcg
     4801 cgtgagcgcc cctgcctgaa aagggtctca accagcagtt tgagtttcgt cccctgccga
     4861 agcgacgcct ttcgcaggcc aacgctgcat ccaatggacg ggtatgggtc ggcaagctcg
     4921 tagatgcctg tgtcggggtc cttgtaggcg ccgtccccaa aaatcagcac ctcgatatcc
     4981 ttgccggtcg cctccctgac tcggtctcgg atggtgtcgc acacgtcatc ggcgttatcg
     5041 ggcaggagct tgagtatgcc cgcgtctagg tccgacacgt tggacccaat cactccccac
     5101 gggccgggcc ccacgtcctt gacagtcagc atgggcgtct tggtgccgaa cgacaggaac
     5161 aggtttttca gcgattcgcg ttcgtgtact gcgccgatga ttatcccgtc gatgtggccg
     5221 tagttgaaca ccgccaacgg gttattggta tagagaatgt cgcaccgggc gccctcggcc
     5281 tcaatagcct tggcgtacat acgtgcatag tcgattccgg tgatcgggtg agggaacagc
     5341 ctctcgccga tctcgccaag ggtgatcacg tcgcgtgcgt cgtacagggc aagcctgtcg
     5401 ctgtacgggt atacatgcgg tttcgcggcg ccttcaagga gaccggccgc atccacaata
     5461 gagatctcgc gagtctgtaa gtccacggca gccacaagcg caccgtctgc atcgctgtct
     5521 tgggctatgc gaagaacctt ctccgcagtg ccgggcatgt cttcgaaagt cacctcagcg
     5581 accaggtggc gtccctcggg gtcgtggagt gtaatgtcgg cgatgccctt acccgtgatc
     5641 ttgcgtatgg cagtcaccgt gtatcctagc tcctggaatt tcagagccgc aagtatctcc
     5701 cttatcaaca cgttcatctg aggcgtattg cccctcacct caagcaggct tccaagggtt
     5761 ctcttgaggc ggaaacggct attggcgaag tcttcgtcca tcacctggtt gccaacctca
     5821 tcatacggca cgctcagctg gacgatgacc tttccacgcc ggacagcacg ggctatggcc
     5881 gatagcacca gcacaaagcg gtttcggctc acgatggggc ttatgaccgc caacgtaccc
     5941 tcttcgctga cagagagttt ggagcgcagg tcctgcacca gctcgtccac ggttacatag
     6001 cggttctgcg aacgggcgac aaccgcctcc gttacgcaga ctatgtcgcc atcctggacc
     6061 gtctctttga ccgtatcaag ggtgatctcc acaatgtcat cagtgggcat gatcaggccc
     6121 gttttcagtc caaccaaggc gactcctgca tgttgcggta ggtcaaaact cacagggttc
     6181 acttcccttc agtagggctt gcgcgcacgc aacaaattat attatcgcac aaggttgcct
     6241 ggactacaat ccaggaattg ccgggatgga attgcccgga tatcggcccg gggactgccc
     6301 ctgagactgc ccgaaggcca cccgaaggct acccgaaggc cgccaggaaa ttcgaggggc
     6361 atctctagaa acgctgagcg tcgctcactg cgcagtcggc ctgatgctct ctcctgcagc
     6421 ggctttccgg attttccggt atatcgtgtt gcgcgaaacg ccgagttcac gggcgacgcg
     6481 gctgatgttg cccccgtgtt tctgcatgag ggtcaacagc tgctcctgtt cgcgccgctc
     6541 aagcattcgc cgcatgcgac gcctctcatc ctcgatgttg gcgaatgccg ctggttgcgc
     6601 atcttccggc gaatggcaca taatctcctc cggaacatgc tgcaacccga tcctgccgtc
     6661 agctgcaatg ctaaccatac gctcaactac gttctgcagc tcacgtacat tgcccggcca
     6721 atcgtagttc atcaagcact ctacaacgcg cgggtcgacg ctaccgacct ctgagcccag
     6781 ccttctacct gccttggcca tgaagaagtc gaacagcaac ggtatgtcgt ccggccgctc
     6841 gcgcagcggc ggcagctgga tctgtatcac gttaagtcta tagtacaggt cctgccggaa
     6901 gcttcccttg gctacggcct gcagcagatc cttgttggtg gcgctgatta ccctcacgtc
     6961 tacctgcgtg acagcgcttc cgcctattcg gatcacgcgc ctgtcctgca agactcggtg
     7021 caaagccact tgctgctcaa gcggcatgtc cccaatctcg tcgaggaata aggtgccgcc
     7081 tgcagccaac tcgaatttgc ccgggcgccc gcctcgcgcc gcccctgtga aggccccttc
     7141 ttcatagcca aacagctcgc ttccaacgag ttcgcggggg attgcgccgc agttgaccgg
     7201 aacaaatggt ccgcacgcgc gtttgccatg gttatgtatc gcctgggcaa tcatctcttt
     7261 tccggtgccg ctttcgccgc atatcaacac actgctgtcg ctctgcgctg caatagaagc
     7321 gacctccacc gctcggcgca gcttctcatc gcgccccagt atgtcgtcaa acctgaatgt
     7381 tgcatacgat ccgctatacc tgctgatgag cttggttatt cgcttggacg gattcagcga
     7441 caccacgcct ccgttgacgt tgccgtgatc atcgtaaacc ggcttggccg ccactaggca
     7501 atgcactggt ccgctgggcg tcttgagcgc catctccacg tcgtcgaagg ctttgcccgc
     7561 tccaagcatg ttcgtaaagt agtgcggctt gttcacgagc tcatacacag gcgcagtgta
     7621 cagcccgtcc gggcccgcgc ggccgccagc gtggccctcg gtgtgggcgt cggtgtgggc
     7681 gtcggcgcca gagtgggcat cggcgccggg gtcgcccatc ggtgctccca ggattccttc
     7741 ggccaccggg ttggcgcgac ggatcatgcc gcggtcgtcc agaagtagta ttccgtccga
     7801 ggttatcagg aacatcttgt taaggttgtc gttgagcacc gtaagctcgc gcatcttcgc
     7861 gttgatacgc atctgagcgc gaatggcttc cgccgctgct actaccatcc cgagagtgtg
     7921 tgcgtgagtg cgcgtggacg ggcccgacat ctgcagagct ccgatcgcgt gcccgcgttc
     7981 gtcgagtata ggcgccgcag agcatgtcca agtgtgaaat cgttcgcagt agtgctcgta
     8041 gccggaaacc tgaaggggag ccatgcatgc gagggcagtg ccgattccgt tagtacccac
     8101 gtgatgttcg gaccagctcg atcccggcac gagattgacc agaccggcac ggtcgagggc
     8161 atcctcatcg ccgattatct ccatcactcg tccccgctcg tcggccagga ttactatgaa
     8221 gccagacccc acgacgaacc tatgcaggct ggacatgaat gggcgcgcta ccctaatcag
     8281 ctcacagtga tcatccagca gatcgtcaat accccgccgg ccggctggga ggcggcccag
     8341 agcgtccttt ggatcgacca tggcctcgct tgaccgtctc catgatgccg ctatctccgg
     8401 acgaaggata gacgagtcta cttcccccgt gttgacaaag tgccgccatg cctcggcaat
     8461 acgtggttgc tgcacaatct cggaagccat ctgcttctca ccgccgattc gtcattttcc
     8521 ttcctgctac taccgattct gcattgcccg ccaaggccct gcctgcctgg gcagctaccg
     8581 gccagactgc agcggtagtg taccttttgt gtacctttag cgtaccgcag tagcaacact
     8641 atagcaacat aaacggtaca tatccgcgct ggacatcatc tgtgcctatt cggccgcggg
     8701 caccggcacg ccccgatcca tctgcgcatt tcatgccgag gatccgtgac tccttttgat
     8761 ggcacattac ttgcttcgat atgcggtcgg atcgggtcga ttccatacgt caattcggaa
     8821 gccatgaatt cggagggagg atctagtcat gcacaagcct gtggacaggt actacaagct
     8881 gttcatcgac ggtacgtggg tagacagtaa ggggggcaag acgttcgaca catattgccc
     8941 tgccaacgga gagttgcttg ccacgtgcgc agacgcaggc aaggaagacg tggacctcgc
     9001 cgttgggtca gcgtggaagg cctttgacag ctggaagaac acgagtcctg tagagcgctc
     9061 cacgattctg ctgaagatcg ccgacctcat cgatgcaaac gcagagaagt tggccatggt
     9121 agagaccatc gacaacggca aacccatccg ggagaccatg tcggtagacg tacccctggc
     9181 ttccgaccac ttccgctact tcgccggcgt cattcgcgct gacgaaggca gcgctgtgat
     9241 gatcgacaag gaccacatga gcatcatcct cagcgaaccg attggggtcg taggccagat
     9301 agttccgtgg aacttcccgt tcctcatggc tgcatggaag atcgcaccgg cgcttgcggc
     9361 aggctgcacc gtggtgatca agccatcgtc gacgacaccg ctctccaccc tggaattcgc
     9421 caaactactg caggaggtag tcccgcctgg agtggtcaac gtagtcaccg gtcgcggttc
     9481 aaccaccggc gagtacatgc tcaaacaccc cggattccgg aagcttgcgt tcaccggctc
     9541 tactgaagtc ggctacgaca tcgcacgggc agcagccgac aagctaattc cggccaccct
     9601 cgagctgggc ggcaagtcag ccaacatcta cttccccgat tgcccgtggg acaaagccat
     9661 cgaaggcgta caggtcggta ttctgttcaa ccaggggcaa gtatgctgcg ccggctcgcg
     9721 ggtcttcgtc catgaagaca tatacgacaa gttccttgcc gaatgcgtcg ccgcgttcaa
     9781 caaggtgaag gtgggcctgc cgtgggaaga ggacaccatc atgggctcgc aggtgaacga
     9841 gactcagctc aagaagatat tgtcgtacat cgacattggc aagaaggaag gtgcccgcgt
     9901 ggcctgcggc ggtgagcgga tcaccgacgg cgaactggcc aagggtgcat tcatgaaacc
     9961 cactatcctt gcagatgtca cgaacgacat gagagtggcg cgtgaagaga tattcggtcc
    10021 ggtagtgtgc ttcatcaagt tccgcgatga agacgaggtc gtcaaaatgg ccaacgacaa
    10081 cgaatacggc ctcggcggcg ctgtgtggac ccgtgacata aaccgcgcac tgcgtgttgc
    10141 ccgagccgtg gagaccgggc gcatgtggat caacgactac aacaacctgc ccgcccatgc
    10201 cccgttcggc gggtacaaga agtcgggcat aggcagagag acacacaaga tgatgcttgc
    10261 tcactacaca cagaagaaga acatcttcat cagcacgagt gaggccaaga agggcatgta
    10321 ctaagtccta aggtcgcggc attggatcgc taaacgcgcc ctgagagtgg gcctcagcgt
    10381 cggcctcagc gtggacctga gagtggtacg gcccggtgtg ctgcggagca gatgcagccg
    10441 ccgggccgac ctctcagcag gggcggtctc tcagcagnnn nnnnnnnnnn nnnnnnnnnn
    10501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10561 nnnnnnnnnn nnnnnnntca gcaggcatag cacttctcca gcctgccaag ctcatcggcc
    10621 caggccagcg aatcagcgta tgcttcgggg agaaacgcct ggtggattcc aagagccggc
    10681 gcagtggctt ctacctgttc ccactgggcg ttttcatagg ccgtcaccat ctgcagcaca
    10741 ggtagaaacg gaccagcctc gccgagcagc gccgtgcgcg tatcttccgt gagcgggagc
    10801 aggtccacaa cttcactcat ggggcgatcg agcagagcgt ccatcaaaga gagcagtccg
    10861 gtcaggaaaa gctccgacgc ccgatgggcg aggcctgcag gggtggctag aagctcggtc
    10921 attcgtgcgc gcacaatggc cgcctcagcc aaagcccttg gtttctcgcc gctaagccca
    10981 gtaacggtaa gtaccgcgat ccagcggcga atctcactga gccccatgat gacaagagca
    11041 tgcttaatcg aggtcacccg gtggcgaagc ccgaacgcgg cagagttcac cacgcgcagc
    11101 agcctgtacg agagggatac atcgcgcgag ataatcgagg caagggtatc gaaatccggc
    11161 gacggatcgt gcacctgtcg gagcagatgg aggctcgcaa gggcatcagg gggaatgtcg
    11221 gaacccttcg cgatttcggg tcgactcagg aagttgccct gaaagagacg gtatccgtgc
    11281 ctcacggccg tctggaactc atcccgcgtt gccaccttct cggctagaaa gacagtgttg
    11341 ggcttgacgg cggatcgagg aatcgcggca cgctgggcat gcgatgctag caagaagtcg
    11401 actttgacga tgtcgacaag cttcaggaat ggctccagat gcggctgacc aatgtagtca
    11461 tcgagagcga tgctaaagcc ctgctcctta aggcgggcga gcgcgtcaag caccgcagga
    11521 ctaggcatcg tattctccag tacttccacg actacgacat caggcggaaa cagcgacgga
    11581 atcccgtctt gcaggagctg ctgggtgaag ttgacgtgta cgggcctccc gttgctcaag
    11641 cgatctatgc ctatagcgat gaaggaattg accaggagtg ccaaagacgc gtagtctgaa
    11701 tcgctgcacg aaaacacgtc gttcaggctc gaacgaaaca gaagctcgta agctgataca
    11761 ttgaggcggg tatcatatat cggttgccgc gccacataga catccatact ccctcactca
    11821 tttcacatca atccatcagt cctccgttga attcaccgaa tcttccagta ttcctcttcc
    11881 ggacgtccag cgcagattcc agggtatgcg aaaccgcaga gggcccgatc ctgtgcccta
    11941 cgggccaggt ttttcggcca tcctgtgtcg gtttacccag gaccctcgaa aatcctgtcc
    12001 ccca
//
LOCUS       DAOTAC010000041        11880 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_4570_length_11880_cov_4.126427, whole genome shotgun sequence.
ACCESSION   DAOTAC010000041 DAOTAC010000000
VERSION     DAOTAC010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 11880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11880
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4570_length_11880_cov_4.126427"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..1367
                     /locus_tag="PLM74_02975"
     CDS             3..1367
                     /locus_tag="PLM74_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HON42081.1"
                     /translation="MVVDVEKIEVYSHDETSETAYVRMPEVVVKPRDASDVSQILRLA
                     NREMIPVTPRVSGTGLSGGCVPMFGGIVLSMERMNRIIEVDGDNLFIVVEPGVTTGEV
                     QRRAREEGLLYAGDPCSAESSQIGGNLAENAGGNKAVKYGTTSRHVYGLEVVLPDGEI
                     MRLGGKCVKDVTGYDLIHLLVGSEGTLGVITRAWLKLLPLPRFSVALLAPFESMQTAI
                     EVVPRIMTAGGIIPTSVEFMDSPSIRAAELYLNSSLPYSDAGAYIIIELEGNSESQLE
                     SEYEFIGKLCQESGALEVFVADNLSTQDRIWKARKCIAEALRIVSPVYCMEDITVPTS
                     EIPGLIAEITEIAARNGVSIPCFGHAGDGNIHATLLRGDMDEPSWERMRDSLLEEIYQ
                     ATYRRGGNLSGEHGIGAKRLEAWRKFADPVQLRVVKSIKAALDPNGIMNPGKVVDYGR
                     PNPE"
     gene            1716..1901
                     /locus_tag="PLM74_02980"
     CDS             1716..1901
                     /locus_tag="PLM74_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42082.1"
                     /translation="MTIFLALGQYSAMGLTLTAKSIVRFSRIEKDPAFAEYYLLGTLY
                     SMLVALLVGLALKVVLP"
     gene            1898..2536
                     /locus_tag="PLM74_02985"
     CDS             1898..2536
                     /locus_tag="PLM74_02985"
                     /inference="COORDINATES: protein motif:HMM:NF027589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SatD family protein"
                     /protein_id="HON42083.1"
                     /translation="MIPMGEFVAIKMDMRGSRRIGDRERAQEQFLQVMESLNREFAPS
                     IQARFIVTHGDEAQGMLKREFSPNVLAVMERAMEGVYPVELRFGIGIGTLSTPLQPDA
                     IGMDGQAWHAASRALDLARSRRKHVMFSGFGEQTDEQLCALANLLLHLRNGWSPEQRR
                     AIELVDAGMMQSAAAEALGVSESAISQRLAAAGWHFYKDGRDAVISLLRPQR"
     gene            2709..3560
                     /locus_tag="PLM74_02990"
     CDS             2709..3560
                     /locus_tag="PLM74_02990"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HON42084.1"
                     /translation="MSLYCLPLIRGRLPSLRPSEQKVARFIMDNPQAVVNMSSVELGA
                     SAGVSESTVIKCSQRLGFNGFVQLKLALARDLAVAPTTAFGEVEQGDSAEVVIQKVFH
                     ANSTALSDTSKVLDPGQLSAAAALISRARAVSFFGMGASAVVAQDAKQKFMRIGILAE
                     CEIDPHLQLTRVSLLGPDDVVAAISYSGETVDTLEVLRLARGVGAHTICITNHPSSSA
                     TEHSDVILVASAAESGLRGGALASRIAQLSVVDCLFVLVAMERYDNAMTCLVKTRQAV
                     AGRKGRS"
     gene            3563..5260
                     /gene="nagZ"
                     /locus_tag="PLM74_02995"
     CDS             3563..5260
                     /gene="nagZ"
                     /locus_tag="PLM74_02995"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HON42085.1"
                     /translation="MYSPGSANAGLVRALMESMTIEDKIGQMIMVGFDGQSLTPELEA
                     HLNDVHAGNVIFLGRNYADPKQMWGFTNDLQRMASRRSVPVGLLTAIDQEGGIVARLL
                     NGVTVFPGNMALGATGRADYARRAAKVTAEEMLQLGLNMNLAPVLDVNNNPGNPAIGV
                     RSYGESPELVAAMGAKAIAASQSTGVLATAKHFPGKGDVTVDSHIDLPHVPHSRERLN
                     QVELVPFKAAIAAGVGAIMTAHVTFPAIEPDPLLPATMSQRVLQGLLRDELGFKGIII
                     TDDLFMGAISKSYGLAEAAIRSINAGADIVLMCHKPDEQAVAIGAIWEAVRSGRISME
                     RIDSSVRRVLSMKALFGILTPPVLTSMPKGVGSQANRKLALAIARESVTVIQDRDGII
                     PFLLPEDDVGGGASGECGVGRDDSNSVDVLVISPDIKNLTMVEDTGSHGSPLAKAVRM
                     FVPGASEMTVSQSPSDREIADAVTGAARRDLVIVGTNNGHLYPAQAQLVKRVVQAGSP
                     VVVVGMRNPYDVRDFAEVSTYIAAYSYRECSMQAAAEVIFGFTVPSGQLPVTIPGCK"
     gene            5306..6211
                     /gene="murQ"
                     /locus_tag="PLM74_03000"
     CDS             5306..6211
                     /gene="murQ"
                     /locus_tag="PLM74_03000"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007727456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HON42086.1"
                     /translation="MSITEGRNPKARNLDMLSVREILQLMNEEDRTVACAVGAVLDQV
                     AEAVDLVVSSLAVGGRLFYAGAGTSGRIALLDAAECPPTFGTPPELVQVLIAGGETAF
                     ARAREGVEDDFDLGMEDARARRISGLDVVVGVSASGNARYVSGVLTCARREGAATIGL
                     ACNPSSAMDGLVDVAITPVVGPEVVMGSSRLKAGTAQKMVLNMISTASMVKLGRVYQD
                     LMIELRPANLKLAGRMRRIVSIAAGVDDDTAGRLIEQADGNLKAAVLMAKNGGSLDHA
                     MAVLDQTGNNLRAALELVEAENAGE"
     gene            6208..7512
                     /locus_tag="PLM74_03005"
     CDS             6208..7512
                     /locus_tag="PLM74_03005"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HON42087.1"
                     /translation="MTGTAVNVRMAGLTEPIRVVGLMSGTSADGIDAALVEISEQSSP
                     SPQPGLPPELSTGLPAREDSGTLPVHTLSIRTLACVNSPFDAELRERVFELFSPPSST
                     VDKICRINVELGEAFARAALEVIRAAGLEPKDVHLMGSHGQTIQHMPACHATLQIGEA
                     AVIAQRTGITTVSNFRARDMAAGGEGAPLVPYFDWLAFRHPESTRVLQNIGGIANMTV
                     LPAGCGLEDVYAFDNGPGNMIIDYMASALTAGRQRYDEDGAMARAGRVNHELVAQLME
                     HPFILQRPPKTSGREEFGAQFAQDLISRWRRLGLTDEDMVATGTAFTAEAIAWHLKSY
                     VTPRSGLDEVILSGGGAFNPVLVQMIRDRITPIPVVCADEYGVSSDAKEAIAFAVLAY
                     ETMRGRPANVPSATGAAEPVVLGCITPGFRGAQWQYGGLSVR"
     gene            7618..8856
                     /locus_tag="PLM74_03010"
     CDS             7618..8856
                     /locus_tag="PLM74_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HON42088.1"
                     /translation="MERVQVLAGIDVAMGGAGADVGACIGAEAGADAARALAGKRLGL
                     ITNPTGVTRSLDATLDVLHRDGRFRLVAAFGPEHGLRGEAQDAAHIGDFRDERTGLPV
                     YSLYGANRRPTPEALAGLDALIFDIQDAGVRFYTYISTMVLAMRTCAEVGKEFIVLDR
                     PNPISGALVEGGILDKRFTSFVGACPVPVRHGLTVGELAMWANETLGIGCELTVCKMM
                     GWRRTMWADDTGLPWVMPSPNLPSLDTATVYPGACFVEGTNVSEGRGTTRPFELIGAP
                     WIDGRALADLLNDLGLAGCRFRPVFFEPRFSKHAGQVCGGVQIHVLDRDVFRPVRTGV
                     HILAAICKLCPDRFQWIGTPEQQAAGRLHVDLLAGTDELRQALDSGGDRRPIDDLLSK
                     WEADAAGFMKDRERFLLYCS"
     gene            9615..9935
                     /locus_tag="PLM74_03015"
     CDS             9615..9935
                     /locus_tag="PLM74_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42089.1"
                     /translation="MRMLGTYGLVAKIIKEAWNSDHWEPAGFHASRQGAAEYWAHETV
                     HRIGEPYCRLILARSSSMDLRKEKSMMKRVEKARAAIAKEAKRFVVRHLVCKADARRA
                     PSSS"
     gene            10136..10579
                     /locus_tag="PLM74_03020"
     CDS             10136..10579
                     /locus_tag="PLM74_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42090.1"
                     /translation="MTTNLTHEDKYPASSILGEYKQQSRVEARFGFLKSPYLLGQILL
                     QKQSHIEALGYVLLMALLIATLLERRVRRNLETEGTPIMIPGQKKTMRPMARIIVKML
                     DAVQVAYVEHEGEARRVWIDSRHGFDMPRLLRLAFLPPISTRILH"
     gene            complement(10815..11735)
                     /locus_tag="PLM74_03025"
     CDS             complement(10815..11735)
                     /locus_tag="PLM74_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42091.1"
                     /translation="MCRGRLELFGAIVLLVCLLSSEVSASPIETPLLLYLVEVVSVNG
                     ASTRHGWFVVPLGEAFSIRSGQQSLADSFALELRAERIDEHGFVVTMLSIIAIGAQTV
                     AASTSIRCEPGVRTCVGSVSTDWSNAEAYITVGMFDWDDPNAPVVLAANLRHLGNPMT
                     APRRSPRAEIWLQGSSDAGPSPMVCMSVPVGKVVSLTLSVGAASGLTLGISDTVQLRC
                     GLRAFADVVLVRTDRKLEHKRYEHNWQLGLELSDGSALGRASIGGRRSPSSISFDVGK
                     NLTSGIWVAAGIGATHVGPTTCRLGLIVDF"
     gene            complement(11737..>11880)
                     /locus_tag="PLM74_03030"
     CDS             complement(11737..>11880)
                     /locus_tag="PLM74_03030"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42092.1"
                     /translation="TSSDDLREKTKTPVLGDIPLVRLLFQQTSSKQAQSELLIFITAE
                     IVD"
BASE COUNT         2450 a         3267 c         3838 g         2325 t
ORIGIN      
        1 gtgtggtagt tgacgtggag aagatcgaag tgtactcgca cgacgagacg tcggagacgg
       61 cttacgtgcg gatgccggag gtggtcgtca agccgcgcga tgcctcggac gtttcgcaga
      121 ttctgcgttt ggccaaccgc gagatgatcc ctgtaacccc cagagtgtct ggcacgggtc
      181 tgtccggggg atgtgttccc atgttcgggg ggattgtgct gtcgatggag cgaatgaaca
      241 ggatcatcga ggtggacggg gacaacctgt tcattgtggt ggagccgggg gtgaccacag
      301 gcgaggtcca gaggagggcc agggaggaag ggctgcttta cgcaggcgac ccgtgctcgg
      361 ccgaaagctc gcagataggc gggaatctgg ccgagaatgc cggcggcaac aaggctgtga
      421 agtacggcac cacgtcgcgg catgtatacg ggcttgaggt agtgctgccc gatggcgaga
      481 taatgcgtct gggcggaaag tgcgtcaagg acgtgactgg atacgacctg atccacctgc
      541 tagttggttc cgagggcacg ctgggcgtta tcacccgggc ctggctcaaa ctccttccgc
      601 ttcccaggtt cagcgtggcc ctgctggcgc cgtttgagtc catgcagacg gcgatcgagg
      661 tcgttcccag gattatgacc gccgggggca tcatacccac gtctgtggag ttcatggaca
      721 gcccgtccat aagggctgcc gagctctacc tcaactcgtc tttgccatac agcgatgccg
      781 gcgcatacat catcattgag ctcgaaggca actccgaaag ccagctggag tcggagtatg
      841 agttcatagg caagctctgc caggagagcg gggcgctcga agttttcgta gcagacaacc
      901 tgtctacgca ggaccggata tggaaggcgc gcaagtgcat cgcggaggcc ttgaggattg
      961 tgagcccggt gtattgtatg gaggatatca ccgtgcccac gtcggaaatc ccgggcctca
     1021 tagcggagat aaccgagatc gcggctagaa acggggtctc gataccgtgt ttcgggcacg
     1081 ccggcgacgg caatatccac gccaccttgc tccgcggcga catggacgaa ccgtcctggg
     1141 agcgcatgcg cgacagcctg ctggaggaga tctaccaggc gacctaccgt cgcggaggta
     1201 acctctcggg cgaacacggg atcggcgcca agaggctgga ggcgtggcgc aaattcgcag
     1261 acccggtgca gcttagggtg gtcaagtcaa tcaaggccgc tcttgacccc aacgggatca
     1321 tgaacccggg gaaggtcgtg gactatggcc gaccaaaccc agagtaaagc gaaacactcc
     1381 ctgcgaacgt cttcccgaac cattccccat ggatgcgtgt ggttatgctc ggagaggaag
     1441 cgaagcctgg cagcaggaga agagtagcgg ccgacgacag ggccgatggc aaaagctagg
     1501 tgtctcgttt ccgaacactt ttttggcgat gtgtctcaaa aacgagcaat gggtagtgca
     1561 taattgaacg catacacgta gacgattgac ttaagctacg tagcttaata tagtagtatt
     1621 aagccgtcag gcttaatgac ccattagctg ctcggctcgg gctgctgcgc tcgggctggt
     1681 actcccgggc catcggcgcg ctcgagaggt ggataatgac catcttcctg gccttggggc
     1741 agtacagcgc catgggcctt acgctgacgg cgaaatcaat cgtcagattc tccaggatcg
     1801 aaaaggaccc ggcgttcgcc gaatactacc tgctgggcac gctctacagc atgcttgttg
     1861 ccctcctggt cgggctggcg ctcaaggtgg tgctcccatg attcccatgg gcgaattcgt
     1921 cgcaatcaag atggacatgc gcggctcaag gcggatcggc gaccgtgagc gggcgcagga
     1981 gcagttcctt caggtaatgg agtctctgaa cagagagttt gcgccgtcga tccaggcccg
     2041 attcatcgtc acccacggcg atgaggcgca gggtatgctc aagcgggagt tctcgcctaa
     2101 cgtactggca gtgatggagc gggcgatgga aggagtatac cctgtggagc tgcgctttgg
     2161 aatcggcata ggcacactca gcactccgct gcaaccagat gccatcggaa tggacggcca
     2221 ggcctggcat gcagccagcc gtgctctcga cttggcgagg agccggagga aacacgtcat
     2281 gttcagcgga tttggggagc agaccgacga gcagctctgc gctctggcca atctgctgct
     2341 acacctgcgg aacgggtgga gcccggagca gcgccgggcg atcgagctgg tggacgcagg
     2401 gatgatgcag tccgctgcag ccgaggccct cggcgtctct gaatctgcaa tctctcaaag
     2461 acttgctgcc gccggatggc acttctacaa agacggccgc gatgccgtga tcagtctgct
     2521 cagacctcag cgctagccat tggcattgct gccctgccgc tgccctgtag cagccccgtt
     2581 gctaacatgt tgctgccggg ccgccgagtc gatcccgccg agacaataag caaatattgc
     2641 gagtcagcgt ccaagtaaga aggaattgtt ccgtacctgt ggaattgcct actaagaggt
     2701 gttgtgggat gtcgctatac tgcctgccgc tgataagggg acgattaccg tcgctgcgac
     2761 cgtctgagca gaaggtcgcg cgtttcatca tggacaatcc gcaggctgtg gtcaacatgt
     2821 cttcggtcga gttgggggcg tcggcgggcg tcagcgagtc cacggtcatc aagtgcagcc
     2881 agaggctggg gttcaatgga tttgtccagc tcaagctggc gctggcgcgc gatttggcgg
     2941 ttgctccaac taccgccttt ggcgaggtgg agcaggggga cagtgccgag gtggtcatac
     3001 agaaggtgtt ccatgcgaac tcgacggcgc tttccgacac atccaaggtg ctcgatccag
     3061 ggcagctgag cgccgccgcc gctctgatct ccagggccag ggcggtgtcg tttttcggga
     3121 tgggggcgtc ggcggttgtg gcgcaggacg cgaagcagaa attcatgaga atcggcatcc
     3181 tcgccgaatg cgaaatcgac ccgcacctgc agctgacgcg agtgtcgctt ctcgggccgg
     3241 atgacgtagt agcggcgatc tcctactcgg gcgagaccgt cgacactctg gaggtccttc
     3301 gtcttgccag aggggttggc gcgcatacca tatgcatcac caaccacccc agctccagtg
     3361 cgactgaaca ttccgatgtg attctggtgg cttccgcggc cgagagcggc ctgcgaggcg
     3421 gcgctctggc ttcgcggata gcccagctgt cggtggtcga ctgcctgttt gtgctagtgg
     3481 ccatggagcg gtatgacaat gccatgactt gccttgtcaa gacgagacag gcagtcgcag
     3541 gcaggaaggg aaggtcgtag ccatgtactc gcctgggagc gccaatgccg gtcttgtcag
     3601 ggcgctgatg gagtcgatga ccatagaaga caagatcggc cagatgataa tggtcggttt
     3661 tgacggccag tcactcacgc ctgaactcga agctcacctg aacgacgtgc acgcgggcaa
     3721 cgtgattttc cttgggcgca actatgcaga ccccaagcag atgtggggtt tcacaaacga
     3781 tctgcagcga atggcgtccc gccgctcggt tccggtgggg cttcttacag ccatcgatca
     3841 ggaaggcggc attgtcgctc gactgctcaa tggagttacg gtctttccgg gcaatatggc
     3901 cctgggagcc acaggtcggg ccgactatgc gcggcgtgcc gccaaggtga ccgccgaaga
     3961 gatgctgcag ctgggcctga acatgaacct ggctcctgtt ctggacgtga acaataaccc
     4021 cggcaacccc gccatcgggg tgaggtcata cggggagagc cccgaactag tcgctgcaat
     4081 gggcgcgaag gcgatcgcgg caagccagtc cacgggggtg ctggcaacgg ctaagcactt
     4141 cccgggcaag ggcgatgtca ctgtggactc ccatattgac cttccccatg tccctcacag
     4201 tcgcgaaaga ctgaaccagg tagagctggt gcccttcaag gccgcgatcg cggcgggggt
     4261 aggggcgata atgactgccc acgtcacgtt tccggccata gagccggacc cgcttctgcc
     4321 ggcgacgatg tcgcagagag tgcttcaggg tctgctccgg gacgagctcg ggttcaaggg
     4381 catcatcatt accgatgatc ttttcatggg cgccatatcg aagtcgtatg gcctagccga
     4441 ggcggcaatc aggtcgataa acgccggcgc cgacatcgtt ctcatgtgtc ataagcccga
     4501 tgaacaggcc gtcgcgattg gcgcaatctg ggaggccgtg cgctcagggc ggatatccat
     4561 ggagaggatc gactcatcgg tccgccgggt gctctcgatg aaggcgctat tcggaatctt
     4621 gaccccgcct gtgctgacga gcatgccaaa aggcgtgggt tcgcaggcga atcgaaagct
     4681 tgcactggcg atcgcgcgcg agtcagtgac ggttattcag gacagggatg ggattatccc
     4741 attcttgctg cccgaagacg atgtgggcgg tggggcaagc ggggaatgcg gggttggccg
     4801 cgacgacagc aatagcgttg acgtgctggt gatcagcccg gacatcaaga accttaccat
     4861 ggttgaggac accggatccc atggatcgcc gcttgccaag gctgtgcgca tgttcgttcc
     4921 gggggcgtcc gagatgaccg tgtcgcagtc gccgtcggac cgggagatcg cggacgcagt
     4981 aaccggcgcc gcccggcgcg atctggtgat cgtaggcacg aataatgggc acctctaccc
     5041 tgcacaggct cagttggtga agcgggtcgt gcaggccggg tcgcctgttg tagtggtggg
     5101 aatgcgtaac ccgtacgatg tgagggattt tgccgaagtc agcacctaca tagctgccta
     5161 cagctaccgg gagtgtagta tgcaggctgc ggcagaggtc atattcggct tcactgttcc
     5221 atcagggcaa ctgccggtca ccatcccggg ctgcaagtag caccgcacgc ggcgcccgtt
     5281 cgcttgatga aggatgtgag tagtggtgag catcactgaa ggccgaaacc ccaaagcccg
     5341 caaccttgac atgctctctg tgcgggagat cctgcagctg atgaatgaag aagacaggac
     5401 tgtagcatgc gctgttgggg cagtgcttga tcaggtggcg gaagctgtcg acctggtagt
     5461 aagctcgctc gcggttggtg ggcggctgtt ctatgccggc gcgggaacga gcggacggat
     5521 tgcgcttttg gacgctgccg aatgcccgcc gactttcggt acccctccag agctggttca
     5581 ggtgctgatc gccggcgggg aaactgcttt cgcgcgggcg cgtgagggag tggaagacga
     5641 tttcgacctg ggcatggaag atgccagggc aagacggata tcagggctcg acgtcgtggt
     5701 gggcgtgtcg gccagcggga atgcccggta tgtgtcgggt gtgctgacat gcgccaggcg
     5761 tgagggcgcg gctactatcg gtcttgcgtg taacccgtca tctgccatgg atggcctggt
     5821 ggatgtggcc attacgcctg ttgtgggtcc tgaggtagtg atgggatcga gcaggctcaa
     5881 ggcgggcacc gcacagaaaa tggtgctcaa catgatcagc actgcgtcta tggtgaaact
     5941 gggccgagtg tatcaggatc ttatgataga gctccgaccg gcgaacctca agctcgccgg
     6001 cagaatgagg cgcattgtgt caatagccgc aggcgtggat gacgatactg cagggcgcct
     6061 gattgagcag gcagacggaa accttaaggc tgcggtgcta atggcgaaga acggcggaag
     6121 cctggatcac gccatggcag tgctggatca gacaggtaac aaccttcgcg ctgcccttga
     6181 gctagtcgag gcggagaatg ctggagaatg acgggaacgg cggtgaatgt tcggatggcg
     6241 gggctgaccg agccaatcag ggtcgtcggc cttatgtctg gcacgtcggc cgacggcatt
     6301 gatgcggcgc tggtggagat atcggagcag tctagtccat cgccgcagcc gggcttgcca
     6361 ccggagctgt ctaccgggtt accggcccgg gaggattcgg gaacactgcc cgtccacaca
     6421 ttgtccatcc gcacattggc ctgcgtaaac tcgccgttcg atgccgaact gcgggagcga
     6481 gtatttgagc tcttctcgcc gccctcctcg acggtggaca agatatgccg gatcaatgtt
     6541 gagctggggg aggcgttcgc ccgggcggcg ctcgaggtga tacgcgctgc agggcttgaa
     6601 cccaaggacg tccatctcat ggggtcacac ggccagacca ttcagcatat gccggcatgc
     6661 cacgcgacgc tgcagattgg agaggcggcg gtaatcgccc agcgtaccgg catcaccacg
     6721 gtatcgaact tccgcgcacg cgacatggcg gccggcggcg agggcgcgcc gctggtgcct
     6781 tacttcgact ggctggcgtt ccgccatcct gagagcacgc gcgtgcttca gaacatcggc
     6841 gggatagcca acatgacggt gttgccggcc gggtgcgggc tggaagacgt ctacgcattc
     6901 gacaacggcc ccgggaacat gatcatcgac tacatggcga gcgcgctcac ggcaggcagg
     6961 cagcggtacg acgaggacgg cgccatggct agggcaggtc gggtcaatca cgagcttgtg
     7021 gcccagttga tggagcaccc cttcatactg caacgcccgc ccaagacgag cggtcgggag
     7081 gaattcggcg ctcagttcgc gcaggacctg atctccaggt ggaggcgtct ggggctgacc
     7141 gatgaggaca tggtcgccac aggcactgcc tttacggcag aggcgattgc gtggcacctg
     7201 aagagctacg tcactccgcg gagcggcctt gacgaagtga tcttgagcgg aggcggtgcc
     7261 ttcaaccctg tactggtaca gatgatacgt gatcgcatta cgccgattcc tgtggtgtgc
     7321 gctgacgaat acggagtgtc aagcgacgcc aaagaggcca tagccttcgc tgttttggca
     7381 tacgagacta tgcgcggccg cccggcaaac gttccgagcg ccaccggagc cgcggaaccg
     7441 gttgtcctgg gctgcatcac cccgggattc cgcggagcgc agtggcagta cgggggactt
     7501 agcgtacgat gacgaggttc ttcgggcagt gcattcagca cgctgcattg gcaggacagg
     7561 tggggacgag attgtcaacc ggtcagacgg tacatgggcg gtgagggagg cttagtgatg
     7621 gagcgagtcc aggttctcgc tggaatcgat gtggcgatgg gcggcgccgg cgcagacgta
     7681 ggagcctgca ttggtgctga agccggtgcc gacgcagcgc gggctctcgc cggaaagcgc
     7741 ttgggcctca taacaaaccc cacgggagtg actaggtctc tagatgcgac gttggatgtt
     7801 cttcaccgcg acgggcgatt tcggctggtg gcggctttcg gtcctgagca tgggctgagg
     7861 ggagaggccc aggacgctgc gcatatcggc gatttccgcg acgaacgcac ggggcttccg
     7921 gtgtacagcc tgtacggggc gaaccggcgg cctaccccgg aggcactcgc cggcctggac
     7981 gcgctaatct tcgacattca ggatgcgggc gtcaggttct acacgtacat ctcgacgatg
     8041 gtgctcgcga tgcgcacgtg cgccgaggtc ggtaaggagt tcatcgtcct cgaccggccc
     8101 aatccgatat ctggcgcgtt ggtggagggc ggtattctgg acaagcgctt cacctcgttc
     8161 gtaggggcat gcccagtgcc ggtgcggcat gggctgacag ttggagagct ggcgatgtgg
     8221 gccaatgaaa cgctcggaat cggctgcgag ctgacggtgt gcaagatgat gggatggagg
     8281 cgaaccatgt gggctgacga taccggcctg ccgtgggtta tgccttcgcc caacctgccg
     8341 tcgctcgata ccgcgaccgt ctatcccggc gcctgcttcg tggaaggcac gaacgtctcc
     8401 gaaggtcgcg ggacgacccg cccgttcgag ctgatcggcg cgccgtggat cgacggccgc
     8461 gctcttgccg acctgctcaa cgacctgggg cttgccggct gcaggttcag gccggtcttc
     8521 tttgaaccga gattctccaa acatgcaggc caggtatgcg ggggcgtaca gatccacgtg
     8581 cttgataggg acgtcttcag gccggttcgg actggggttc atatcctcgc ggccatatgc
     8641 aagctttgcc ccgatcgatt ccagtggatc ggcaccccgg agcagcaagc cgcaggcagg
     8701 ctacacgtgg acctcttagc tggcacggac gaactccgcc aagcactcga ttccggtggt
     8761 gatcgccgac ccatcgacga tttgctgtcg aaatgggagg cagatgccgc aggtttcatg
     8821 aaagaccgcg agcggtttct gctttactgt agctgagcgg cccttcggtc ccggtttgcc
     8881 aggtttcaga gccgaccggg caaggcgagt aatctaggag agcagcatga ctgcaggatt
     8941 ctgcattctc cgcggtccaa cgcggaggtt ttcttccgtg tacgtgcgaa gagtctccgc
     9001 caggcgggtt ttgggccgcc ataatgtcat aaaccggcac tttgagggcc atcttgctgg
     9061 tctgaccagg tgcgagcttc caacttccgt tccgcatgta acatatggca agcaggcatt
     9121 ttggagatga ccagctaacg acttgtgtgc tctttccggg tagtgtggat gccggacgtg
     9181 catgatactg tacgtggaga ctatcttgga ggtccttagc gcggtaaggc cagtgaccgc
     9241 aggcgcgact tctctgatcg cagccatggc ggatgagatt ggcctcaccg acatcataga
     9301 tggatgctgc gaatgggacg agacgagata cagtatcccg ccggcaaggc catcgagacg
     9361 cttgccatct gcgtcctttc agaacgagag gtgctgtacc gcatcgagta gttccacgag
     9421 ggccaggatg tggaggcgct cttcggaacg ggggtcagag atcacaattt cgacgatgat
     9481 gcccttggca caggccttga ccagttattg gcggccggcg ccaacggtgt tcttggcgag
     9541 gcgatctcgt ccgcgctcga aaagcacggc attacggtgg gttcaactca cttcgatacg
     9601 acgaacctct cggtatgcgg atgctgggca cctacgggtt ggtcgccaag atcatcaagg
     9661 aagcgtggaa ctcggaccat tgggaacctg cgggctttca tgcttcccga cagggagccg
     9721 cggagtactg ggcccacgag actgttcaca gaataggcga gccatattgc cggcttatcc
     9781 ttgcccgctc gtccagcatg gacttgcgca aggagaaatc catgatgaag cgggtagaaa
     9841 aggcccgggc cgccattgcc aaagaagcca agagattcgt cgtgagacac ttggtctgca
     9901 aggcagatgc gcgacgggcg ccgagctcct cgtagacagg cacgctcaac gatttcgcta
     9961 gctatcgttt gaggtaaccg aggaggccgt cagagagaaa cgcattcggc gaggcaggcc
    10021 caggaaagat gagcaaccgc ccgcagagag gacagtgtgt agggcgtcgg tcacaatcga
    10081 cgggacagag caagcccagg tggagaaggc tcgttaacgc gaaagctact tcgttttgac
    10141 aaccaacctg acacatgaag acaaatatcc ggctagcagc atactaggag agtacaagca
    10201 gcagtcaagg gtggaagcga gattcggctt ccttaagagt ccgtacctgc tcgggcagat
    10261 ccttctccag aagcaaagcc atatagaggc cttggggtac gtactgctga tggcgttgct
    10321 catagccact ctactggaga ggcgcgttcg caggaacctt gaaaccgagg gtacgcccat
    10381 catgatacca ggccagaaga agacgatgcg gcccatggcc cggattatcg tcaaaatgct
    10441 cgacgcagta caggtagcgt atgtagaaca cgaaggagaa gcacgccgcg tctggataga
    10501 ttcgagacat gggtttgaca tgccgcgact ccttcggctc gccttcttgc cgccgatatc
    10561 cacacggatc cttcactgag gtggccgacg gacccgtcct cgaatgccaa gatgggagcg
    10621 tctacatgcg gaacgtgggc tacgctcttc acgcgcaagg aagtcgaagc atggttccaa
    10681 agtttgtgga tatcgcgtat ggagtaagga gtagccttga cgaacaacgg cagaccgcct
    10741 ccgccccgtg ttcatacggg ttgtctgggc gtttgtgcac aggtaacaga gcagtactag
    10801 atgggctgaa agcgttagaa atccactatc aggccaaggc ggcatgtagt tggcccgaca
    10861 tgagtggctc cgattccagc cgccacccat ataccactag tgaggttttt gccaacatca
    10921 aaggagatac tcgaggggga tcggcgacct ccaatgctgg ctcgccccaa cgcgctgcca
    10981 tccgatagtt ctaggcccaa ctgccagttg tgctcgtatc gtttgtgctc tagcttccgg
    11041 tcggtgcgca ccagaacaac atcggcaaag gcacgaagtc cacatcttaa ctgcactgta
    11101 tcggagatgc caagagtgag ccctgatgct gcacctacag ataaggtaag tgagactacc
    11161 tttcctacag gtacagacat gcacaccatc ggactggggc ccgcatcgct tgatccctga
    11221 agccagatct cggcccgcgg agatcgtctg ggtgcagtca taggattacc caaatgccgc
    11281 aggtttgcag cgagcacaac cggagcattt ggatcatccc agtcgaacat gcccacggtt
    11341 atgtaggcct ctgcattgct ccaatctgtg gaaactgaac cgacacacgt tcgcacgcca
    11401 ggctcacacc tgatcgacgt acttgcagcc acggtctgag ctcctatggc tataatggag
    11461 agcatagtaa ccacaaaccc atgttcgtct attcgttccg cccgtagttc tagggcaaac
    11521 gagtcagcca gtgactgttg cccggagcga atggagaatg cttctcccaa gggcacgaca
    11581 aaccatccat gtctggtact ggccccattc actgatacga cctcaaccag gtatagaagc
    11641 agaggtgtct cgatcgggga tgcgctcacc tcagatgaaa gtaagcacac cagaagcact
    11701 atggctccga aaagctccag tctgccccta cacatgtcag tccactattt cggcggtgat
    11761 gaatatcagt aactcgctct gcgcctgttt cgaactggtt tgctggaaca gcaagcgcac
    11821 caacgggatg tctcccagga ctggcgtctt ggtcttctcg cgtagatcat ctgatgaggt
//
LOCUS       DAOTAC010000042        11854 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_4586_length_11854_cov_4.217815, whole genome shotgun sequence.
ACCESSION   DAOTAC010000042 DAOTAC010000000
VERSION     DAOTAC010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 11854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11854
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4586_length_11854_cov_4.217815"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="PLM74_03035"
     CDS             <1..470
                     /locus_tag="PLM74_03035"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HON42093.1"
                     /translation="VILIHMPPLRERPDDIPLLALHFAQSYAREMNKCVSLAPSALEL
                     LKQQPWRGNVRELKNVVERAMVFANSHAIDAQELAAHLSGTSVGGSSRVSQSAVATTQ
                     GLPLQQAVAEYRRSYINQALARHGGNVTHAALDLGIARETLHRILRQARDSDV"
     gene            617..2110
                     /locus_tag="PLM74_03040"
     CDS             617..2110
                     /locus_tag="PLM74_03040"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004607041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HON42094.1"
                     /translation="MRDPQSVLKREPYKMYIDGKFVASDSGKTFDVVSPVTNKVIATA
                     YEGGSSDAERAISAARRAFDDGPWSRMSQAKRSSLLLRAGEILKERQDKFMAIEGINC
                     GKLYPSLKYYELPSAIDAFQFAAGKARCLEGRVVPVDGGGHYLNYVMWQPCGVVAEIL
                     PWNGPLMMGCQKVSMILAAGNTVVIKPPTWAPLSLLELASVFHEVGFPEGVVNVVPGP
                     GSSVGDTLVRSPLVDMVSLTGGTETGKQIFAAAADTVKSIALELGGKSPNIVFDDVDV
                     PEVAKWAFFAFTLNSGQVCVAGTRLILHEAIYDGFINELVGLCDQVVPGDGFTPGVNF
                     GPIISRSHYESVWRHIGQGIVEGARLVAGGFRYTDPTLASGNFIPPTIFADVTPDMSI
                     FQQEIFGPVLCVSKFSTESEAIQLANAVDYGLAGGVFTGDAKRAHRVAEKIRAGQVYV
                     NTYYSKGIMESPGIGWKSSGVGGAGITKYMHPKTVFVELEDGAVPPM"
     gene            2135..3481
                     /locus_tag="PLM74_03045"
     CDS             2135..3481
                     /locus_tag="PLM74_03045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:3200..3202,aa:Sec)
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HON42095.1"
                     /translation="MTSLADKLRALCYINQFYAGIGGEDKADIGVSVFEGAKGPSMGM
                     QGMWHHDMEVVATIACGDNFANTESTFPELAAALAEAVDKFKPHVFISGPAFNAGRYG
                     VACARTADYVHSELGVPSVTGMYPENPAVPMFVRHNYIVATPETAAGMAKALPPLASL
                     ALKLAKGEPIGTALEEGYIPTGHRRNELHRLTAATRVVDMLMARLNNKPFVTEIPIRV
                     FETTPAAPRVTDIADSVIALITTGGLVPQGNPDGLRQAFSTTYGSYSLAHLSSMPAGD
                     YESIHGGYDTTMVNADPNRLVPLDALRQLEAESSVGGVWDVFLTTCGIGTNVSSSVSI
                     GRRMAAQIKQAGVRAAILTSTUGTCTRCGATISKELDRAGIPNALITAFTSIAENVKA
                     NRIVFGGHFTSPVGKPGLPAQRELAYRRRIVETALRALATDVQAPTVFTVDEGGEI"
     gene            3484..4800
                     /locus_tag="PLM74_03050"
     CDS             3484..4800
                     /locus_tag="PLM74_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010956676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HON42096.1"
                     /translation="MAGTMRLTRNYYDVKRAVWANKTRLADGVLHINRDEIETLVKPI
                     LGGIAEARFELVAPGENTRIIHVLDTVQPMYKPEGNAYPGIIGPPTTTGSGATNLLRG
                     FVVMESAALPWESSSASSGLLYPRDAIVDMTGPVSEYSPFSHTSNLVMCYNLVEGKSS
                     AEYDAAIRLSAMRVADYLARVTQEAQPDEVELFSLDDAADDLPKVVLVWQCQNQGVYA
                     NTLLYGHPIDNLVPTWLHPNEMLDGAVVSGNYVWPAFKVPTYLHANHPILLELYRRHG
                     VDLNFAGVVFSRSHNPTHWHKERSASFCVKLARMLGAQGLVMAWEGGGNAAVDAMLTI
                     EYAEKLDIAATAVTFEFGGQDGTEGDLLVDSVPEAIAIVSGGSIEKTISLPPVDRVVG
                     SEVLRLNKESGGWFPNAAEAITFDTTTHIYCSGNQSGHGRLYGAAY"
     gene            4946..5074
                     /locus_tag="PLM74_03055"
     CDS             4946..5074
                     /locus_tag="PLM74_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42097.1"
                     /translation="MADVKTEQFGFFWWINWIIRILLWLLTIAIVFWFILRIFGWC"
     gene            complement(5393..9655)
                     /locus_tag="PLM74_03060"
     CDS             complement(5393..9655)
                     /locus_tag="PLM74_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42098.1"
                     /translation="MPHFRTSTARDEVTLMEACCSDGTTGKGARPARATLMVLLLLGL
                     ALIIYPVFGTSAAEILGLPLEIRGDVEFRIRSAWGDPLALTGTGYSPGHVDIAQLLSL
                     SVRGQVAPGISVSGTLDNRKDGNLQLLELTLDGDPFKGKFGGLAYRSENPYTAYTSRL
                     RGLEVRAEYPAVQAGVTVGRVQGIAAKKTFQGSTAQETIVYEPRGTYAPSPASTGLSS
                     SLEGMEYYQLSGVFDPDYMGAWIRYTDCVPLGEERTLKQTLNLWDLGYLYLDGPDGTG
                     VISAGDGVALSRGQYVTVSSSYEMLAMRSEVRDILRGQIQSFIRSYNAKNMLTGSEQR
                     RYPFMYGSETETAFLEDLVGLHARIVAGTAHDDPGALLDAPASSYARRRLYDMGQTDV
                     VSGSVSVEVRRGGQYYPADTELALGFHVMYDTGVIEFDFPATFFNTYDGIRLKYRHTV
                     AQGIFNLGISIALGSEHVYLNDNLLRRDLDYVIDYEFGILTILLPLGPDDVVRVEYEY
                     FRGPFGAPADYKSNFIGAVLGWTPNEQVKVRMEIARYADDAKSAALPDATPVMPNTHT
                     VMGLSGKFESKGLSISGDLAVSHDEFPFDDNRKPKAANAVLGVMAAEDANGKPYLVIS
                     HRDGMSIGIVGTGAFRSYGIGSGLAGPVVRTMASSGDVWFFATDGGLTVLSAVPGPAG
                     QSPFDYVGNWRRLYTSAGLPSNNLTSVALTPLSVWVGTADDGLATAYLDDLETWTVYK
                     KSSVTGLLSNSISGIAYDPIQDVIVVGTECGLASLHGGHFDVERAGVNVRAVVSGAEA
                     VGGARTFAATDEGVYARNDSGQWSLVVSGAAVADATAIAVWGGVLWIGTGDGLYAWDG
                     SEAVPVDATLGYSVTGLGVGPGFKYDGQALWAGTEARPDADTGKGYALAVFEVVKVDV
                     AVRHSGSDLEISAEDPRRYLNISPEDHTVTGYAARASARYAIGAGSIYGTYEAVTPGF
                     SKLGQTSRQGLDTWRIGAQWPLGSKASISAEHSQSRVQAYSGRPDQVEPKELFTISSR
                     LAGSLDIGPKIDVSYTTSKVDDAAAEGFEREERTISVGGRQAFFEGALTLGAGYDNTE
                     SLSTAVPSSSYTQIAMRGDATLKLDALSVTARYRKPVKTISPGKPEERTSGNEEISLN
                     AQWAKQLGSVSVRASYRQLNRTDIATERKLDDKRADVRATLPALKLGAGTLTPSAVLR
                     WEQVVPYSGQLRRVAGAQASLSGVLGLLRTTSGAGLTRTEYPDIHKVTLDSEAFLTMG
                     GGVVATFAPQVDIRWKRSTSQRPDLGDVSTDSLTGTVRGAWTPGQGLSNVASASYTLS
                     ASAGGQKHMLSLSDSLSAAIGSKATATVDVSATSTAPAVGQLLGGEKAEVRGDIKGGV
                     RYKITDVWSVGVTAGYSLRLLSTGAERLKSAVTFEAGLRAIF"
     gene            complement(9782..10675)
                     /locus_tag="PLM74_03065"
     CDS             complement(9782..10675)
                     /locus_tag="PLM74_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42099.1"
                     /translation="MRTKYADWLIVVALAAMVVLAAPTAFAQTDEDPVGTGALFDIGM
                     GARALGMGGAFVAVADDANALYYNPAGLALIEGRKVTSLYSTLMGAGSYFGAGYAQKN
                     IGAAVFGLMSTVEKTDQYGNPTGELGYREGTLVGGYARAFGPFALGGTLKLYAQEADA
                     NRGFGATCDVGALMSLPYMGGIRLGAVARSMLGTIEFVSGHKDNFDPSYVLGLSLTPI
                     KGLLVAADYDVTHSVGRLGAEYQITDVFAARAGASANADKQFGFTAGAGLAYQSFRFD
                     YAYQFHPELPDTHWLSLGFSF"
     gene            complement(10950..11375)
                     /locus_tag="PLM74_03070"
     CDS             complement(10950..11375)
                     /locus_tag="PLM74_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42100.1"
                     /translation="MMNIKPRAAGGYITLLLVIIALMAGYVVSSYYMPDVSFVDPWQL
                     IAFELLLYVTVCYLWDRRSGAPEAFLKALGMVAGRLGLAALSALAIMRYWPGFASCKA
                     AFAQAVLASQPVVSIQIAYTVLLFALPFRVLRKRDWTSW"
     gene            complement(11372..>11854)
                     /locus_tag="PLM74_03075"
     CDS             complement(11372..>11854)
                     /locus_tag="PLM74_03075"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HON42101.1"
                     /translation="PGFAFAVSATTMVGQALGAGRPDVAERAGWQSWKMSATVMGLMG
                     AVLFFFPGILMRLYVDDPEIIALGAGVLRIMACAQIPMSAAYVFMGALRGAGDTKSVL
                     YVAMSSVWLVRFSLAALFVNVFGWGLAGAWYAMALDWVVRGSVSMARFAGGRWKEMTV
                     "
BASE COUNT         2548 a         3612 c         3382 g         2312 t
ORIGIN      
        1 gtgtgattct catacacatg ccgcctctcc gtgaacgccc ggatgacatc cccctgctgg
       61 cactgcattt cgcccaatcc tacgcgaggg agatgaacaa atgcgtaagc ctcgctccct
      121 ccgccctaga gcttctcaaa cagcagccct ggaggggaaa tgtgcgcgaa ctgaaaaacg
      181 tcgtagaacg cgcaatggtg ttcgcgaatt cccacgcgat agacgcgcag gaactggcag
      241 cacacctatc gggcacctca gtcggaggtt catcacgtgt gagccagtcc gctgtggcca
      301 cgacacaggg cctccccttg cagcaggccg tcgccgagta ccgccggtcc tacatcaatc
      361 aggcactagc caggcatggc gggaatgtca cccatgccgc tctggatcta ggcatcgcca
      421 gagagaccct tcatcgaatc ctccgtcagg cgcgtgacag tgatgtgtga cacgtaggtc
      481 acacatgtga tattcattcc acagccggtc tcccctcctc tcaccatcag cccacgtata
      541 cactgtacgg tagttggcac gcaccttgcg ttcattgact attagctaca gctatggcca
      601 aaagggaggt tcgcatatgc gtgatccgca aagtgtactg aaacgtgaac cgtacaagat
      661 gtacatcgac ggcaagttcg tagcttctga ctccggcaag acattcgatg tagtcagccc
      721 tgtgaccaac aaggtaatcg ctacagccta tgagggaggc tcttcagacg ccgaacgcgc
      781 aatatctgca gctagacgcg cgttcgatga tggcccgtgg agccgcatga gccaggcgaa
      841 acggagcagc cttctgctgc gcgcaggcga aatcctcaag gaacgtcaag acaagttcat
      901 ggccatcgag ggtataaact gcgggaagct gtacccgagc ctgaagtact atgaactgcc
      961 ctcggccatc gacgcttttc agtttgcggc aggcaaggcg cgctgccttg agggcagggt
     1021 tgtgcctgta gacggcggag ggcactacct gaattatgtg atgtggcagc cgtgcggagt
     1081 tgtggcggaa atcctgccat ggaatgggcc gctcatgatg ggttgccaga aagtcagcat
     1141 gattctggcg gccggaaaca ccgtagtgat caagccacca acttgggcgc ctctctccct
     1201 gctggaactg gcctcagtgt ttcacgaggt ggggttcccg gaaggcgtag tgaacgtggt
     1261 tccgggcccg ggctcaagtg tcggagacac actagtaagg agcccactgg tggacatggt
     1321 ctctctcact ggtggcaccg agacaggcaa acagatattc gcggccgcag ccgataccgt
     1381 caagagtatc gcgctcgaac tcggcggaaa gagcccgaat atcgtattcg acgatgtgga
     1441 tgtgcccgag gttgccaagt gggcgttctt cgcgttcaca ctcaactccg gccaggtgtg
     1501 tgtggcgggc accaggctga tactgcatga ggccatttac gacgggttca tcaatgaact
     1561 ggtgggccta tgcgatcagg ttgtacccgg cgacggcttc acacccggcg tgaacttcgg
     1621 cccgattatc tcccgctcgc actacgagag cgtatggcgc cacattgggc agggaatcgt
     1681 ggaaggcgcc cggctcgtcg ccggcggttt ccgctatacc gacccaacgc tggcttcagg
     1741 caacttcata cctcccacca tattcgccga cgtaacgccc gacatgagca tattccagca
     1801 agagatcttc ggacctgtac tatgcgtgag caagttcagc accgaatcag aggcgatcca
     1861 actggccaac gccgtcgatt acgggctagc cggcggtgtt ttcacaggcg acgcaaagcg
     1921 cgcccatcgc gtggcagaga agatcagagc cggtcaagtg tatgtgaaca cctattacag
     1981 caagggcatt atggaatcgc ccggtatagg atggaagtcc agcggcgtag gaggtgcagg
     2041 aatcacgaaa tacatgcatc cgaagacggt attcgtggaa cttgaagacg gcgccgttcc
     2101 gccaatgtaa ctgaacctca ttcggaatgg aggagtgaca tcattggcag acaagcttag
     2161 agccttgtgc tacatcaacc agttctatgc cggcattggc ggggaggaca aggctgatat
     2221 tggagtaagc gtctttgagg gcgcaaaggg cccctccatg ggtatgcagg gcatgtggca
     2281 tcatgacatg gaggttgtcg ccactatcgc ttgcggcgac aacttcgcta atacggaaag
     2341 caccttcccg gagctggctg ccgccctggc cgaagcggtc gacaagttca aaccccacgt
     2401 gttcatatcg gggccggctt tcaacgctgg gcgctacggg gtagcgtgtg cgcgaacggc
     2461 ggattacgtc cactcggaac tgggcgttcc cagcgtcacc ggcatgtacc ctgagaaccc
     2521 ggcggttccg atgtttgtga ggcacaacta catcgtcgcg acgcccgaga ccgcagcagg
     2581 aatggcaaaa gccctcccgc cccttgcatc gctagcgctc aagctggcca agggcgagcc
     2641 cataggcacc gccttggagg aaggatacat tccgacagga catcgtagaa acgaattgca
     2701 ccggctgaca gctgctacac gcgtcgtgga catgctcatg gccaggctga acaacaagcc
     2761 cttcgtcacc gagataccca tacgcgtttt cgagaccaca cccgccgccc cgcgagtcac
     2821 cgacatcgca gactcggtta tcgcactgat caccacaggc ggactcgttc cccagggcaa
     2881 tcccgacgga ctgaggcagg cgttctccac cacctacggg tcgtactcac tcgcgcacct
     2941 gtcgtctatg ccggccggcg attacgaatc gatacacggc ggctatgaca ccaccatggt
     3001 aaacgccgac cccaaccgac tggtcccttt agatgccctc agacagcttg aggccgagag
     3061 cagtgtaggc ggcgtgtggg acgtgttcct cacaacatgc ggcataggca ccaacgtgag
     3121 cagttcagtg tcgataggaa gacgtatggc cgcccagatc aagcaggccg gcgtccgtgc
     3181 agctatcctt acatccacct gaggtacatg tacacgttgc ggtgcaacga tatccaagga
     3241 actagacagg gctgggattc cgaacgccct tattacggca ttcacatcca tcgccgagaa
     3301 cgtaaaagcg aacaggatcg tattcggagg gcatttcacc agccccgtag gcaaacccgg
     3361 ccttccggcc caacgtgaac tagcctacag aagacgtatt gtggaaactg cactcagggc
     3421 gctggccact gatgtacagg cgccaaccgt gttcacagtg gacgaagggg gagagatcta
     3481 gccatggctg gaacaatgag gctcacgcgg aactactacg atgtgaagcg cgcagtctgg
     3541 gccaacaaaa ccagacttgc agacggtgtt ctgcatatca accgcgacga aatcgaaacg
     3601 ctggtgaaac cgattctggg cggaattgcg gaggcccggt tcgagctggt ggctcccgga
     3661 gagaacaccc gaataatcca cgtacttgat acggtacagc ctatgtacaa gccggaaggc
     3721 aacgcatatc cagggatcat cggcccgccc actaccacgg gctcaggcgc caccaacctg
     3781 ctccgaggct ttgtcgtgat ggagagcgcc gccctaccgt gggaatcgtc cagcgcgagc
     3841 agtggtctgc tgtaccccag ggacgcgata gtggacatga cggggccagt atctgagtac
     3901 agcccattct cccataccag caatcttgtg atgtgctaca acctagtgga gggcaaatca
     3961 tcggctgagt atgatgcagc gataagactg tcggcgatgc gtgtagccga ctatctggcc
     4021 agagtcacac aagaagccca gccggacgag gttgagctat tctcgctaga cgatgcggcg
     4081 gacgaccttc ccaaggtggt gctcgtatgg caatgccaga atcagggcgt ttacgcaaac
     4141 acattgctct atgggcatcc cattgacaat ctggtaccca catggctgca ccccaatgag
     4201 atgcttgacg gagccgttgt gagcggcaac tacgtgtggc ctgccttcaa ggtccccacg
     4261 tatctgcatg caaaccaccc aatactgctc gagctctatc ggcgacatgg cgtagatctg
     4321 aactttgcag gcgtcgtctt cagccgcagt cacaatccaa cccactggca caaggaacgt
     4381 tcggccagct tctgcgtgaa actggcgcga atgctcggcg cccaaggcct tgtgatggcg
     4441 tgggaaggcg gaggcaacgc cgcggtagat gcaatgctta cgattgagta tgccgagaag
     4501 ctcgacattg ccgcaacggc cgtaacgttc gagtttggag ggcaagacgg cactgaaggc
     4561 gaccttctgg tagacagtgt gcccgaggcc atcgccattg taagcggtgg aagcatagag
     4621 aagacgatat cactcccgcc agtggaccga gtagtgggca gtgaagtgct gcggctcaac
     4681 aaggagtcgg gaggttggtt tccgaacgcg gcggaagcga tcacattcga taccaccacc
     4741 cacatatatt gctccggcaa ccaatcgggc cacggcaggc tgtatggagc tgcatactag
     4801 ccacctttat caggcacagt ggtatgtcct atccattgac cacctgcccc ttgactgaac
     4861 tgcttcgagg tcaggacatc cgtcatatgc gcaattcgtg gcagacgcat aacatgaaac
     4921 acagcaaacg cggaggtgat gacccgtggc agacgtcaaa accgaacaat tcgggttctt
     4981 ctggtggatc aactggataa tccggattct gctctggctc cttaccatcg caatcgtgtt
     5041 ctggttcatc ctcaggatct tcggctggtg ctaatcggcc gcagatagta gttatttagc
     5101 ggaggtgagc cccctatggc agaattgaaa actgagtgcc tgtttctccc gtggtggctg
     5161 tggtggcttc tagtccttac ggccatttcg ttcctgatct ggttcgtgct ctgtttgttc
     5221 ttcggaatct gctagtaggt gcgtccgtgc ggcctcgggc cccttgcacg aatctgccgc
     5281 cactgacacc tgatccgcag agtggactgg gctcctagcc cagtccactc gcgtcaatca
     5341 tcccaaaaac atcgtccgac aaaccccagg cgcgagcagg atcaggcgga tgctagaaga
     5401 tagccctcaa cccggcctcg aaagtcacgg ccgatttcag ccgttcggcg ccggtggata
     5461 gcaatctgag agagtagccc gccgttaccc caacggacca cacgtcggtg atcttgtacc
     5521 taacccctcc ctttatgtct cccctaacct cggccttttc gccgccgagc agctgaccga
     5581 cggcaggcgc tgttgacgtt gccgacacgt ccaccgtagc ggtggccttg ctgcctatgg
     5641 ctgcgcttag gctatcggaa agactgagca tgtgcttctg gccgcctgcc gaggccgaca
     5701 gggtgtagga ggcggacgca acattggaga ggccttgccc gggtgtccat gcgcctctca
     5761 ctgtgcctgt cagcgagtcg gtactgacat ctcccagatc cgggcgctga gaggtggaac
     5821 gcttccacct gatgtcgacc tgcggcgcga acgtcgcgac gacgccgcct cccatggtca
     5881 ggaacgcctc ggaatccagg gtaaccttgt gtatatcagg gtattccgtt ctagtcaggc
     5941 ccgctcctga cgttgttcgg agaaggccta gcacgccgga gagactggcc tgcgccccgg
     6001 cgactcgccg caactgcccc gagtagggga ccacttgctc ccatcgcagc accgcggatg
     6061 gggtgagcgt cccggccccg agcttcagtg caggcaacgt ggctctgaca tcggctctct
     6121 tgtcgtctag cttccgctca gttgcaatat cggttctgtt gagctgccta tagctcgccc
     6181 tgacactgac cgagccgagc tgctttgccc actgggcgtt aaggctaatc tcctcgttcc
     6241 cgctcgtgcg ctcttccggt ttaccgggcg agatggtctt gaccggcttg cggtacctgg
     6301 cggttaccga taacgcgtcc agcttcagag tcgcatcgcc tcgcatagcg atctgagtgt
     6361 acgagctgct gggtacggct gtgctaaggc tttcggtgtt gtcatagccc gcgcccagcg
     6421 tgagtgcgcc ttcgaagaat gcctggcgcc cacccaccga tatggtacgt tcttctctct
     6481 cgaaaccctc ggcggcagcg tcgtcgacct tggacgtggt gtacgacacg tcgatcttcg
     6541 gcccgatgtc aagagaaccc gccagcctgc tggatatcgt gaacaactcc ttgggttcga
     6601 cctgatcagg tctgcccgaa tacgcctgga cccgcgactg cgagtgttcc gccgaaatgc
     6661 ttgccttaga ccccaagggc cactgcgccc cgatgcgcca ggtatctagc ccttggcgcg
     6721 acgtctgccc tagcttgctg aaacctgggg taacagcctc gtaagtaccg tatatgctgc
     6781 cggcgccaat cgcgtatcgg gcgctggcac gcgccgcgta tccggtaact gtgtggtctt
     6841 ccggcgagat gttcaggtag cgtcggggat cctccgcgga gatctcgaga tcggatccgc
     6901 tgtgtcgcac agccacgtcg accttcacta cctcaaagac cgcaagtgcg tagcctttgc
     6961 ctgtgtcggc gtcaggacgc gcctcggtgc ctgcccacag agcttgtccg tcgtatttga
     7021 agccagggcc gaccccaagg cccgtaacag aatatcccaa agttgcatcc accggcacgg
     7081 cctccgagcc gtcccatgca tacaggccat cgccggtgcc gatccagagc accccgcccc
     7141 atactgcgat tgccgttgca tccgccacgg ctgcacctga cacaacaaga ctccattggc
     7201 ccgaatcgtt ccgtgcgtac acaccttcgt cagtcgcagc gaacgtgcgc gcgccgccta
     7261 cggcttcggc tcctgacacc acggctcgaa cgttcacgcc agcccgctca acatcaaaat
     7321 gccctccgtg aagcgaggcc agtccgcatt cggtgccaac tactattacg tcctgaatcg
     7381 gatcgtatgc gatgcccgat atggaattcg agagcaatcc ggtcaccgac gacttcttgt
     7441 agactgtcca tgtctccaga tcgtccagat acgctgtggc cagaccgtcg tcagcggtgc
     7501 caacccaaac tgacaacgga gtaagcgcca cgctcgtcag gttgttgctg ggcagcccgg
     7561 cagatgtata cagtctacgc caattgccaa catagtcgaa cgggctctgc cctgcgggcc
     7621 ccggcacggc tgaaagcaca gtcagaccgc cgtctgtggc gaaaaaccac acatcgccgc
     7681 tgctcgccat ggtgcgcaca acaggcccgg ccaaccctga accgataccg taggagcgga
     7741 aagctccagt tcccacaatt ccgatactca tgccgtcgcg gtgcgaaata accagataag
     7801 gtttgccgtt agcgtcttcg gcagccatca ctcccagcac agcgttggcc gccttgggtt
     7861 ttcggttgtc gtcgaagggg aactcgtcgt gactcacagc caggtcgccc gagatgctca
     7921 gccccttgct ttcgaacttg cccgaaagcc ccatcacagt gtgggtatta ggcatcacgg
     7981 gtgtagcatc aggcaatgcc gccgatttgg cgtcatccgc atagcgcgca atctccatcc
     8041 tcaccttgac ctgttcgttc ggggtccaac ccagaacggc cccgatgaaa ttcgacttgt
     8101 agtcggctgg cgcgccgaac ggccccctga agtactcgta ttcaaccctc accacgtcat
     8161 caggccccag cggcaatagg atagtgagga tgccgaattc gtagtcgatc acgtaatcca
     8221 ggtcgcgccg gagcagattg tcgttgaggt atacatgttc gctgccgagc gcgatggaga
     8281 tacctagatt gaagatgccc tgggccacgg tgtgccggta cttcaggcgt atcccgtcgt
     8341 acgtgttgaa gaaggttgct ggaaagtcga actcaatcac gcccgtgtca tacatgacgt
     8401 gaaatcccaa cgccagttcg gtgtcggccg ggtaatactg ccctcctctg cgcacctcta
     8461 cactgactga cccagacacc acgtctgtct ggcccatatc gtacaggcgt cgccgcgcat
     8521 atgaggatgc cggcgcatcc agaagcgcgc cggggtcatc atgggctgtg cctgccacta
     8581 ttcgcgcgtg caggccgacc aggtcttcaa ggaaggcagt ctcagtctcg cttccataca
     8641 tgaacggata tcgtctctgc tcgctgcctg taagcatgtt cttggcgttg tatgaacgta
     8701 tgaacgactg gatctgtccc cgcaggatat cacggacctc gctgcgcatt gccagcattt
     8761 cataggagga cgaaaccgta acgtactgcc cgcgggatag tgctacccca tctccggctg
     8821 agatgacccc cgtcccgtcc gggccatcca ggtacaggta tcccaggtcc caaaggttaa
     8881 gggtctgttt tagggtacgc tcttccccga gcggaacgca gtccgtgtaa cgtatccagg
     8941 cgcccatgta gtccgggtcg aatacacctg agagctggta gtattccata ccttcgaggg
     9001 aagacgagag ccccgtcgac gcgggcgacg gcgcatacgt acctcggggc tcatacacta
     9061 tcgtctcttg agcagtacta ccctggaagg tcttcttggc cgcgatgccc tgcactcttc
     9121 ctacagtaac accggcctga actgcagggt actcagcgcg tacctccaga ccacgcagac
     9181 gagacgtgta ggcggtatag gggttctcac ttctgtacgc aaggcccccg aatttgcctt
     9241 tgaacgggtc gccgtccaga gtcagctcca acagctgaag attgccatcc ttgcgattgt
     9301 cgagtgtccc cgataccgag atcccgggag caacctgccc tctgacactc aacgacagaa
     9361 gttgtgctat gtcgacgtgc ccggggctgt aaccggttcc cgtgagcgca agcggatctc
     9421 cccaagccga gcggatcctg aactccacgt cgccacgtat ctctagaggc aagcccaaga
     9481 tctcagcggc cgacgtccca aagacagggt agatgataag tgccaaccca agcaacagaa
     9541 gcaccataag ggtcgcgcgg gcaggacgag caccttttcc ggtggtcccg tcagagcagc
     9601 aggcctccat cagcgtcacc tcatcacggg cagttgaagt gcggaagtgt ggcacgatcc
     9661 ccctcgttta cctttccgag ggcgagatgt acctacatgg atgattccac agaaaaggag
     9721 ggtccccttt cgtcaataat cggaagcccc tccatgcgtc attagcggac gcgagtccgc
     9781 cctagaacga aaagcccaac gatagccagt gggtgtcagg cagctcgggg tggaactggt
     9841 aggcgtagtc gaacctgaag ctctggtagg cgagcccagc accggcagtg aaaccgaact
     9901 gcttgtcggc gtttgcgctc gcgccggccc gagctgcgaa aacgtcggtt atctggtact
     9961 cggcgcccag gcggccgact gagtgcgtaa cgtcgtaatc agcagctacg agcaatccct
    10021 tgataggcgt gagcgacagc ccaaggacgt acgacgggtc gaaattgtcc ttgtggccgc
    10081 tcacaaactc gatggttccg agcatgctgc gggccacagc gccgagcctg ataccgccca
    10141 tgtaaggcag actcatcaac gcacctacgt cacaggtggc gccgaagccc cggttggcat
    10201 cggcctcctg cgcgtagagc ttgagagttc cgcccagggc aaatggccca aaggcgcgtg
    10261 cgtaccctcc caccagagtg ccctcgcggt agcccagttc accggtggga ttgccgtact
    10321 ggtcggtttt ctccactgtc gacattagcc cgaacacagc cgcgccgatg ttcttctggg
    10381 catagcccgc gccaaagtag ctgcctgccc ccatcagtgt cgaatagagc gacgtcacct
    10441 tgcgcccctc tattagggct aggcctgcgg ggttatagta cagagcgttg gcgtcgtcgg
    10501 ccacggccac aaacgccccg cccataccga gcgcccgagc gcccattccg atgtcaaaca
    10561 gtgcgccggt gccgacagga tcctcgtcgg tctgtgcaaa agccgtcggc gccgcaagca
    10621 ccaccatcgc cgccagcgcg actacgatta gccagtcagc gtatttcgtt ctcatttcgt
    10681 cacccccggt tagcagctaa tgaccagaat gctcagctag atgagctttt agttgtcgat
    10741 gtggtgggta tacatggtcc acctgcacta tattgctata cttcaacgga gaactcagtt
    10801 caccttctga taggcgcggt agccgggggg attcttcggt tgagtccggg aggatggcag
    10861 tggtgtgcgc gagtacctgg ggcggatgga tgatgaggcg acgggccggc gccgcgtgcg
    10921 gctcggcagg cagaaggcag acgatccgac taccagctgg tccagtctcg tttgcgcaga
    10981 actctgaagg gaagagcgaa gagcagcacg gtgtaggcta tctgtatcga cacaacgggt
    11041 tgggaggcga gaaccgcctg ggcaaaggcg gccttgcaag acgcgaatcc cggccagtat
    11101 ctcatgatcg cgagggccga gagcgcggcg agacctagcc tgcctgcaac catccccagg
    11161 gctttgagaa aggcctccgg ggcgccggac cggcggtccc acagataaca aacagtcaca
    11221 tatagcagaa gttcaaaggc gatcagctgc cacggatcaa cgaaggatac atccggcatg
    11281 tagtagcttg agacgacgta tcccgccatg agggctatga tgacgaggag gagtgttatg
    11341 tagccccctg ccgctctggg cttgatgttc atcataccgt catttccttc catctgccgc
    11401 cggcgaagcg tgccatcgaa accgacccgc gcacgaccca atcgagggcc atcgcgtacc
    11461 aagcgccagc caggccccat ccaaacacgt tcacaaacag cgcagccagc gagaaccgga
    11521 ccagccatac gcttgacatg gcgacgtata gcacggactt ggtatccccc gctccacgca
    11581 gcgcccccat gaacacatat gccgcagaca tgggaatctg ggcgcaggcc attatccgga
    11641 gcacaccggc gcccaaggcg atgatctcgg gatcgtccac atagagccgc atgagtatcc
    11701 ccgggaagaa gaacaagacc gcacccatga gccccattac cgttgcgctc atcttccacg
    11761 actgccaccc ggcacgctcc gcaacgtcgg ggcgcccggc tcccagcgcc tgccccacca
    11821 tggtagtggc cgacaccgca aacgcgaacc ccgg
//
LOCUS       DAOTAC010000043        11809 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_4604_length_11809_cov_4.778629, whole genome shotgun sequence.
ACCESSION   DAOTAC010000043 DAOTAC010000000
VERSION     DAOTAC010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 11809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11809
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4604_length_11809_cov_4.778629"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="PLM74_03080"
     CDS             complement(<1..749)
                     /locus_tag="PLM74_03080"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HON42102.1"
                     /translation="MSRRKNELIPVIAVVAALAVVAIVWVVVTRATAVAIVNGQRITR
                     REFVTRLEQVTGEQVLSDLINEQLIGQAAKKAKVTVTSAEVEAEMEKLREQIGPSFDS
                     VLAQYGMTVEDLRKNMELNLLVYKVSTKDITVSEDALKAHFEENKSDYDQPEMVKASH
                     ILVDTEEKAKEIQKKLAEGADFAELARSESTDPMSAAEGGDLGFFARGRMVPEFEKVA
                     FSMSPGQTSGPVKSEYGYHIIRVTERKDAHEA"
     gene            complement(909..3170)
                     /locus_tag="PLM74_03085"
     CDS             complement(909..3170)
                     /locus_tag="PLM74_03085"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF000907.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase/30S ribosomal protein S1"
                     /protein_id="HON42103.1"
                     /translation="MSVRTPRIRLAEHAGFCAGVERAVGIARETGISAMRAGYAGVVT
                     LGPLVHNPAVVASLAHCGVQQVSSVDEARGRVAVIPSHGLSPAVVQDAREKGLSLVDA
                     TCPHVTRVQREVADAHRGGYFVVLVGDELHPEVRAVVPFAEGGIAVVSGAREAAAMEL
                     PKEPITVVAQTTQTASTVDAVVAAIAARGKQVRLVNTICPATSLRQEAARKLAREVDL
                     MVVVGGRTSANTMRLAEVCSDTGVRVLKIEKADELDVEAVRDSGTIGITAGASTPSWV
                     IEEVMDAMNEIIEGNPDEICRSEQGAEQAETVDRVEQAEPAEPVDHVEQVEQAEPAEP
                     VDQVEQAEPAEQADTHAEAAEESSPGESREEPKPAAPTHVGPPEPEVLKARVEEIYDD
                     RVIVQIETGEMATIVTKSLTADAIAHPSEVVALGEEIYVVLERHSRGSDTLFASKRQA
                     DRLLLWKRLEHARDTGETISGKVTEAVKGGLVVDIGVRGFVPASQVGRRFVEDLSTYV
                     GEELKLKVREVERLRSNVVLSQRDYLEEQEKLARERMFDTLERGQVICGTVTRLAVFG
                     AFVDIGDGVEGLLHISDIAWSRIKHPGEVLSENQPVEVVVLNVDRERQRISLGYKQLQ
                     PDPWENVSKRFPVGSVVSGTVTKLVEFGAFVSLAEGIEGLVHISQLADRRVARPDEVV
                     SVGETVTIKVIGLKEQEHRISLSIRQALEDGERNEYRKYMKDNRSDDGVTIGDRIAYD
                     PSKFKSGADEDDE"
     gene            complement(3167..3814)
                     /gene="fsa"
                     /locus_tag="PLM74_03090"
     CDS             complement(3167..3814)
                     /gene="fsa"
                     /locus_tag="PLM74_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012811990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HON42104.1"
                     /translation="MKLFIDSANIEEVRQAAEMGIISGVTTNPSLVAREGRDFRQVVT
                     EICGLVDGPVSAEVISLDAAGMVHEAIDIAAWSPNVVIKVPMTAEGLKAVHQLTAMGV
                     KTNVTLVFSSNQALLAARSGATYVSPFVGRLDDISSDGCDVVAETAAIFDMHDIDTQI
                     IAASIRHPLHITRAALAGADIATVPYAVLTQMIKHPLTDAGVARFMADWEKARRK"
     gene            complement(3836..4447)
                     /locus_tag="PLM74_03095"
     CDS             complement(3836..4447)
                     /locus_tag="PLM74_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HON42105.1"
                     /translation="MFYAIVRRLIRVYFHLFYGFKVTGLENVPTAGAIIVAANHASAL
                     DPLALGAACPRPLRFMAKAELFDNPILALILRGLRAFPVKRGQVDREAYRTSMETLRA
                     GDALGMFPEGTRSKTGELQAAHPGAARFSMHTGAPIVPAAVVGTFAGHRKGMNRFREA
                     KVRVVFGAPVHPRAPVEGRITKEDSDRLAHQIMERIQDLINAA"
     gene            complement(4429..5121)
                     /gene="cmk"
                     /locus_tag="PLM74_03100"
     CDS             complement(4429..5121)
                     /gene="cmk"
                     /locus_tag="PLM74_03100"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HON42106.1"
                     /translation="MSLERSIAIDGPAGSGKSTVARMLARALGYRYVSTGDLYRALAL
                     EVLRRGVDPHDEESVVKVAQSVSMSVEPTPGGAQRTIVGGEDVTALIRSAEVDSIVSP
                     VSVYPAVRAHMVKLQRAIAQDADVVMDGRDIGSVVLAESRCKFYLTASPQERAARRQR
                     DYAAQGRRVGLDTVCEEIAQRDMIDSTRRVSPLAVVPDAIVIDCTAMSPAEVVTAMMQ
                     VVGGADTCSTQL"
     gene            complement(5212..5703)
                     /locus_tag="PLM74_03105"
     CDS             complement(5212..5703)
                     /locus_tag="PLM74_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42107.1"
                     /translation="MSRRSDIATTMLTAAVCTLIALCVTAQWMIASGRISRANLLVER
                     AEGQVVWTWGSSGSDGSIPVGPAPESGGLLLSLSEPSESIVVLLNGEAVADFRYKRAA
                     IVVHRGDMVEIDAGQGPWGDGQATTLLQVEIADTTPNIRAPRRGMRIQLSPGRTAVAN
                     IVL"
     gene            complement(5705..6970)
                     /locus_tag="PLM74_03110"
     CDS             complement(5705..6970)
                     /locus_tag="PLM74_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HON42108.1"
                     /translation="MSNVVAVIGAGAAGMMAAGAAAGAGASVTVYERNTVAGKKILVS
                     GKGRCNFTRDCSAQELVEGFGPRGKFLYSALSSFGPAQMCALMENLGVPYKVERGRRV
                     FPASDRASDVRDALYMRAAESGARFVLGRRVSRLAFEGGAVVGVIDTRDAIFRADAVV
                     VATGGASYPATGSTGDGYALARQVGHSVNEPMPALVPMETVEEWPRPLAGLALRNVEV
                     TLYDRGRTRGREFGEMLFTHYGVGGPIILTLSRDVCQAQRSRREVEPGRFTLGIDLKP
                     ALAEATLDRRIQRDLAAMSRKQLNNALDMLLPKALIPAVIRFAEVDETTPANQVTAAQ
                     RAALVGALKDLRLTVLRPRPLEEAIVTSGGVELSEVDPRTMQSRLAPGLFFAGEVLDV
                     DGPTGGYNLQAAFSTGYLAGISAARQRRG"
     gene            complement(6981..7718)
                     /locus_tag="PLM74_03115"
     CDS             complement(6981..7718)
                     /locus_tag="PLM74_03115"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HON42109.1"
                     /translation="MTTERIAKFLARSGVASRRACEQLVASGRVTLNGVLVTTPATFI
                     DPERDVVAVDGQAVHPATDHIYLALNKPRGVVTTARDTHHRPTVLDLIPDMGRRIYPV
                     GRLDMDSDGLLILTDDGELAFGLTHPSRHAPKTYLVRLQRRVTSDDLARLAHGIRLDD
                     GPTLPAKARLADGTGKLIEIEMHEGRKRQIRRMFASLGNRVFRLTRTRFGCVELGRLL
                     EGSYRELTREEVDGLKRLLQQSAAEGR"
     gene            complement(7715..8365)
                     /locus_tag="PLM74_03120"
     CDS             complement(7715..8365)
                     /locus_tag="PLM74_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019005614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HON42110.1"
                     /translation="MAQQFAVIGLGRFGMSVARTLSELGQEVFAIDVDEDKVQRIAPV
                     VTHAVVADVTDIEAMRSIGIRNVDVAVITVGSEVKASMVGTMVLRELGVRTIVAKAND
                     DLHGKVLEKLGADRVVYPERDGGSRLARVLTSTNLIEQIDLDPDFSIVEMTAPSSLAG
                     KSLAELSVRARFGVYVMAIRSGDVMVVAPGADDVIHADDVLVVIGRNDRLQRFRKA"
     gene            complement(8492..8890)
                     /gene="ytfJ"
                     /locus_tag="PLM74_03125"
     CDS             complement(8492..8890)
                     /gene="ytfJ"
                     /locus_tag="PLM74_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HON42111.1"
                     /translation="MPDHPIDGLMKTTLDSIGQMIDVNTILGDPVETPDGNVIMPVSR
                     VTFGFAAGGSDWAAESAPASNSSPFGGGGGAGISLNPVGFLVVGNKQVRFLPVTTDAV
                     FDRLLDVVPDVLTRMGAIRPQPDTVKPPLL"
     gene            complement(8943..9560)
                     /locus_tag="PLM74_03130"
     CDS             complement(8943..9560)
                     /locus_tag="PLM74_03130"
                     /inference="COORDINATES: protein motif:HMM:NF022615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HON42112.1"
                     /translation="MRWAQVGLTAGATLAVVLAMVLYWPFRLDLSIARDSGRTRATVR
                     VWPWPFFIFVPFYRRDVPPPTVPTARWTLSDLTETFVRLRIMARVITSAASLGHRLTE
                     LSWHSSIGTGDAASTAIACGELWAVKSVVAAWACSRWGPAHKIPSFSVTPVFGKAQFS
                     TSFHLVVETALATLLLDARLRAALLSLVATLRKRRANELSSGLEY"
     gene            complement(9591..10121)
                     /gene="scpB"
                     /locus_tag="PLM74_03135"
     CDS             complement(9591..10121)
                     /gene="scpB"
                     /locus_tag="PLM74_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HON42113.1"
                     /translation="MPQTNLHEAQAAIEAILFAAAFPLSVKDLAAAIQIDEDTTAKLL
                     RELGDRHSVPESGIVLSKLDGSFILMSRPEYAPFVENLSARQRPAPLSPASIETLSII
                     AYRQPVTRGEIERIRGVSSDSSIATLSERGLIAEVGRRNSVGRPVLYGTTPEFLMYLG
                     LTSLSDLPPLPESEDQ"
     gene            complement(10099..10848)
                     /locus_tag="PLM74_03140"
     CDS             complement(10099..10848)
                     /locus_tag="PLM74_03140"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HON42114.1"
                     /translation="MKVDTEGFSGPLDLLLSLIEKKQIDICEVSISDIADDYLEQVSA
                     MEWLDIDQAAEFLIIAATLLYIKVRALLPPEEVPEELAAATSEMTDPRAELVARLVEY
                     RRYRDAARVLRNLEAVGSRMYTRPLRPLPTPTVRANPVEGLTGHDLLQAYLDASSSIP
                     EEFPYLPADEIPIATQMVSVLVRLARKGKLGLFEFLGGARSRHLLVSTVLAVLELVRR
                     GRISASQPAPFGEIWMFATSQAIGGAADELT"
     gene            complement(10845..>11809)
                     /gene="trpS"
                     /locus_tag="PLM74_03145"
     CDS             complement(10845..>11809)
                     /gene="trpS"
                     /locus_tag="PLM74_03145"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004095957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HON42115.1"
                     /translation="FSGMRPTGMLHLGNLAGALANWVRLQDDYECFFAAVDWHALTTG
                     YQDTSMIREYTRQVLIDFISAGIDPERSIVFRQSDVKEHAELHLLLSMITPLGWLERC
                     PTYKEQLQQLAGREITTYGFLGYPVLMASDILVYKADTVPVGEDQLPHLELAREIARR
                     FNYMYAPVFPEPQAKLSKVAVLPGTDGRKMSKSYGNTIQIADPPEKIRQAVQSMVTDP
                     ARIHKSDPGHPDVCTVFAYQSAFAPESAEGRRGECEGAEIGCVACKRQLADTLISVLA
                     PIHERRRQLEATPGLVESIIADGAMRARPIAARTLEEVRSAMRI"
BASE COUNT         2292 a         3803 c         3297 g         2417 t
ORIGIN      
        1 gcctcatgcg cgtctttcct ttcggtgaca cggatgatgt ggtatccgta ctcgctcttt
       61 accggcccgc tagtctggcc cggcgacatc gagaaagcaa ccttctcgaa ctccggcacc
      121 atacgcccgc gcgcgaagaa cccgaggtcg ccgccttctg ccgccgacat cggatcggta
      181 gactcgctgc gtgccagttc agcgaagtcg gccccttcag ccagcttctt ctgaatctcc
      241 tttgccttct cctctgtgtc gacaaggata tggctggcct tcaccatctc aggctggtcg
      301 tagtcggact tgttctcttc gaagtgtgcc ttcagggcat cctcgctcac agtgatgtcc
      361 tttgtcgaca ccttataaac cagaagattc agctccatgt tcttccggag gtcttcgacg
      421 gtcatcccat actgggccag caccgagtcg aaactgggcc ctatctgctc gcgtagcttc
      481 tccatctccg cctcgacctc ggcggaggtc acagtcacct tggccttttt ggccgcttgc
      541 cctatcagct gctcgttgat gagatccgac agaacctgct cgcctgtgac ctgctcaagc
      601 cgggtgacga actcacggcg cgtgattctc tggccgttga cgatggcaac agccgtcgct
      661 ctagtgacga cgacccacac tattgcgaca acagccagag ccgccaccac tgcgatgact
      721 ggaataagtt cgttcttgcg cctagacatg ctttggcatc cccctttgca actccgtccc
      781 aagtgacatc ccacctagcc ttaacgacgc ctgccctcgg caagttccgg gggcagacgg
      841 ccagagctag tctagcacat ggtaatttga gtgtcaacga aaccccatat agaaggcttt
      901 ataagggact actcgtcgtc ttcatcggcg ccgcttttga acttgctggg gtcataggct
      961 atccggtcgc ctatagtaac gccgtcatca gaccggttat ctttcatgta cttgcgatac
     1021 tcgttccgct ccccgtcctc aagtgcctgt cggatcgaga ggctgattcg gtgttcctgc
     1081 tctttcaggc ctatcacctt gatagtgact gtttcgccta ccgacaccac ctcgtccggc
     1141 ctcgcgacgc gcctgtcggc gagctgcgag atgtgcacta gcccttctat accttcggcc
     1201 aggctgacaa atgcgccgaa ttccactagc ttggtcactg tcccgctcac cacagaaccg
     1261 accgggaacc tctttgatac gttctcccac gggtccggct ggagttgctt gtagccaagg
     1321 gaaatcctct ggcgttcgcg gtcgacgttc agcaccacga cctccacagg ctgattctcc
     1381 gatagcactt cgcccggatg cttgatcctc gaccatgcga tatcggagat gtgaagcagc
     1441 ccttctactc catcgccgat gtcgacaaac gcgccaaaca ccgcaagccg ggtcacagtg
     1501 ccgcaaatca cctggccgcg ttcgagtgtg tcaaacatgc gctcccgggc aagcttctcc
     1561 tgttcctcaa ggtagtcgcg ctgcgacaat accacgttgg agcgaagcct ctcgacttcg
     1621 cggaccttca atttcagctc ctcacctaca taggtgctga gatcctcaac gaatctccgc
     1681 cctacttgcg acgccgggac gaatccccgc acgcctatgt ctacgactag cccacccttg
     1741 acggcctcag tgaccttgcc cgaaatggtc tctccggtgt ctctggcatg ctccagtcgc
     1801 ttccacagaa ggaggcggtc cgcttgcctc ttcgaggcga acagagtatc tgaaccccgg
     1861 ctgtgccttt ccaagactac gtatatctcc tcgcccaaag cgaccacttc agaggggtgg
     1921 gcgatcgcgt cggcggtgag gctcttggtc acgatagtag ccatctcgcc cgtttcgatc
     1981 tgaacgatga cacggtcgtc gtagatctct tcaacacggg ctttaagcac ttccggctcg
     2041 ggcggcccca catgtgtggg tgcggcaggt ttaggctcct ctcgggactc gcctggcgac
     2101 gattcctctg cagcctcggc atgcgtgtcg gcctgctctg ccggttctgc ctgctcgacc
     2161 tggtctaccg gttcggccgg ttcggcctgc tcgacctgct caacgtggtc taccggctcg
     2221 gccggttcgg cctgctcgac ccggtctacc gtttcggcct gttcagctcc ctgctccgac
     2281 cggcaaatct cgtccgggtt cccttcgata atctcgttca tagcgtccat gacctcctcg
     2341 attacccaac tgggcgtgga tgctcctgcc gtgatgccga ttgtgcctga atctcgcaca
     2401 gcctcaacgt cgagttcgtc ggccttctcg atcttgagca ccctgacgcc ggtgtcactg
     2461 catacttccg ctagccgcat ggtattggca ctggtccgac ctccgactac gaccatgaga
     2521 tcgacctcgc gtgcaagctt cctggcagcc tcctgcctca gagatgtcgc cgggcagatt
     2581 gtgttgacca gcctgacctg cttgccccgg gcggcgatgg ctgcaaccac cgcatccacg
     2641 gtcgatgccg tctgcgtcgt ctgcgcaact acggtgatcg gctccttcgg tagctccatg
     2701 gccgcagctt cacgggcccc cgagactacg gctattcccc cctccgcgaa tgggacaacc
     2761 gcacgcacct cgggatggag ctcatcgcct acgagcacta cgaaatagcc gcccctatgc
     2821 gcatccgcca cctctctctg cactcgcgta acgtgcggac aggtggcatc taccaatgac
     2881 aatcctttct ctcgcgcgtc ttgcacgacg gcgggcgaaa ggccatgcga cgggatcacg
     2941 gcgacgcgcc ctcgggcttc gtcaaccgaa ctcacttgct gcacaccgca gtgtgcaagg
     3001 cttgcaacta cagcagggtt atgcactaga ggccccagcg tgacaacgcc ggcgtacccg
     3061 gcgcgcatag cgcttattcc ggtttcacgc gcgatgccaa ctgcacgttc cacccctgcg
     3121 cagaagcctg catgctctgc caacctgatc cggggagtac ggacgctcac tttctgcgtg
     3181 ccttctccca atctgccatg aacctcgcca cgcctgcatc agtcagaggg tgtttgatca
     3241 tctgagtcaa aaccgcgtac ggcaccgtcg ctatgtcggc tcctgccaac gcggctctgg
     3301 taatatggag cgggtggcga atgctcgcag caatgatctg agtatcaatg tcatgcatgt
     3361 cgaaaatggc cgcggtttca gcgactacgt cgcagccgtc ggagctgatg tcgtccagcc
     3421 tgcccacgaa tgggctgacg taagtcgcgc ccgatcttgc tgccagcaag gcctggttcg
     3481 atgagaacac cagagtaaca ttggtcttga cgcccatggc ggtcagctga tgcacggctt
     3541 tcagcccttc tgcggtcatc ggcaccttga tgaccacatt gggagaccag gctgcgatgt
     3601 caatggcctc gtgtaccatt ccggcggcgt caaggctgat cacctcggcg cttaccggcc
     3661 catctaccaa tccgcagatc tccgttacca cctggcgaaa atctcggccc tcccgagcca
     3721 caagggacgg gttggtcgtc actccgctta tgattcccat ctcagctgcc tgacgtactt
     3781 cttcgatatt agcgctatcg atgaagagct tcaaatcggt acactccctg caatttcatg
     3841 ctgcattgat caaatcctgg attcgttcca ttatctgatg cgcaagtcgg tcagagtctt
     3901 ccttcgtgat acgcccctct acaggagctc tggggtgaac cggggcgccg aatactactc
     3961 tcaccttggc ttcgcggaat ctgttcatac ctttacggtg cccggcgaac gtgccgacga
     4021 cggccgcggg cactatcgga gccccggtgt gcatcgaaaa cctggccgct cccgggtgcg
     4081 cagcctgtag ttcgccagtc ttgctgcgcg tgccttccgg gaacattccc agggcatcgc
     4141 cggctctaag cgtctccatg gaggtacggt aagcttcacg gtctacctgc cctcgcttga
     4201 ccggaaaagc gcgaaggccg cggagaatca gagccaggat cggattgtca aacagctctg
     4261 ctttggccat gaaccgcagc ggcctcggac atgccgcgcc gagcgcaagg gggtcgaggg
     4321 cgctggcatg attagcagcc actatgatag caccggccgt cggcacgttc tccaggccgg
     4381 tgactttgaa accgtagaac aggtgaaagt agactctgat cagccttctc acaattgcgt
     4441 agaacaagtg tcggcccccc cgaccacctg catcatcgcg gtcaccactt ccgcggggct
     4501 catcgcagtg cagtctatta cgattgcgtc gggcaccacg gcaagcggag atacccggcg
     4561 agtgctgtca atcatgtcgc gctgggcgat ctcctcgcac accgtgtcca gcccaacccg
     4621 cctgccctgc gcagcgtagt ctcgttggcg tcgcgcagca cgttcctggg gcgatgctgt
     4681 gagatagaac ttgcagcgcg actcggccag tactacactg ccgatgtcgc gcccgtccat
     4741 gactacatcc gcatcttggg ctatcgcgcg ctgaagcttg accatgtgcg cgcgaaccgc
     4801 cggatacaca gaaaccgggg agacgatgga atccacctcc gcgctgcgta tcaacgcggt
     4861 tacgtcttcg cctccaacta tggtgcgctg agcgccgccc ggcgtcggct cgacgctcat
     4921 cgataccgac tgcgcgacct ttaccaccga ctcctcatca tgtggatcca cgcctcgcct
     4981 caatacctcc agggcgagcg cacggtacag atcgccagtg ctcacatatc tataccccag
     5041 ggcccgggcc agcatgcggg cgactgtgct tttgccggag cctgcaggcc catctattgc
     5101 aatcgaacgt tcaagcgaca tgcgtcggac tctcctatcg gtgcacaatt cactgttatt
     5161 ctacatccga accgctattc cttcgccagt ggctcgaaat atgattcgaa tctataatac
     5221 aatgttcgct acagctgttc taccaggcga cagctgtatg cgcatgcccc gcctcggcgc
     5281 ccgaatgttg ggagtcgtgt ccgcgatctc cacctggagt agggtagtag cctgcccgtc
     5341 gccccaaggg ccttggccgg catcgatttc aaccatgtcg cccctgtgaa caactattgc
     5401 ggcgcgcttg tacctgaagt ccgcaactgc ctcgccgttc aggagcacga cgatgctctc
     5461 cgatggttcg gacagagaca gcagaaggcc ccctgattca ggcgccggcc ccacgggaat
     5521 gcttccgtcg ctcccgctgg aaccccatgt ccacacgacc tgaccttcgg ccctctcaac
     5581 aaggagattt gcgcgcgaaa tgcggcctga agcgatcatc cactgcgcgg ttacgcacaa
     5641 cgcaatgaga gtgcaaactg cagcagtgag catagtagta gcgatatcgc tgcggcgcga
     5701 caattcagcc tctcctctgc cgagccgcgg agattccggc cagatagccc gtggagaagg
     5761 cggcttgcag gttgtagcct ccggtgggac cgtccacgtc gagcacctct ccggcaaaga
     5821 atagacccgg cgcaagcctg gattgcatgg tacgcggatc gacctcgcta agttccaccc
     5881 cgcccgaggt cacgatggcc tcctccaacg gacgcggcct aagcactgtg aggcgcaaat
     5941 ccttaagcgc gccgaccaga gctgcccgct gtgccgccgt gacctggttg gcaggagtag
     6001 tctcgtccac ttcggcaaag cgtattacag ccgggatgag cgcttttggc aacaacatgt
     6061 cgagggcatt attcagctgc ttccgcgaca tggcggccag gtcgcgctgg attcgccgat
     6121 ccaaagtcgc ctcggcaagc gccggtttga gatcgatacc aagagtgaaa cgaccgggct
     6181 cgacctcccg gcgagacctc tgggcttgac atacgtcacg gctcaacgtg aggatgatcg
     6241 ggccgcccac gccataatgc gtgaacagca tctccccgaa ttcgcggccg cgcgtgcgac
     6301 cgcggtcata caatgtaacc tccacgttcc gaagcgccaa gcccgcaagg ggcctgggcc
     6361 actcttcgac cgtttccatc ggtaccaggg caggcatggg ctcgttcacc gaatgcccca
     6421 cctgtcgggc cagcgcatat ccgtcgcccg tgcttccggt ggcagggtag gacgcgccgc
     6481 cggttgcaac gacaacagca tctgcgcgaa atatcgcgtc ccgggtatcg attactccaa
     6541 caaccgcccc gccctcaaat gcaagccggg aaacgcgtcg gcccaacacg aagcgcgcac
     6601 cactttccgc ggcgcgcatg tacagggcat cgcgtacgtc tgacgcgcgg tcgcttgcgg
     6661 ggaataccct tctgcctcgc tcgaccttgt agggcacgcc gaggttctcc atgagcgcgc
     6721 acatctgggc aggcccgaaa gatgatagtg ctgagtacag gaacttgccg cgaggaccga
     6781 agccctctac tagttcctgt gcggagcaat ctcgggtaaa gttgcatctg ccctttcctg
     6841 acacaagtat cttctttccg gccaccgtgt ttcgctcgta caccgtgacg cttgctcctg
     6901 cgccggccgc tgcgccggca gccatcatgc ctgcggcgcc cgctccgatt actgccacaa
     6961 cattcgacat cgccaagcca tcagcgccct tcagcagcgc tctgctgcaa cagtcgtttg
     7021 agcccgtcga cctcctcgcg cgtaagctcg cggtagctgc cctctaggag gcggcccagt
     7081 tccacgcagc cgaatctggt acgtgtcaga cggaagaccc ggttgcccaa agaagcgaac
     7141 atacgccgga tctgacgttt cctgccctca tgcatctcta tctcgattag cttgccggtt
     7201 ccgtccgcaa gtcgggcttt ggcggggagc gtagggccat cgtctaggcg gatgccgtga
     7261 gcgaggcggg ccaggtcgtc agaggtcaca cgcctctgca gcctgaccag gtacgtcttt
     7321 ggtgcatggc ggctggggtg ggtcagcccg aaagcgagtt ctccgtcgtc agtcaagatc
     7381 aaaagaccgt cggagtccat gtccagcctg cccacgggat agatcctgcg gcccatatcg
     7441 ggaatgagat cgagcacggt ggggcgatgg tgagtgtcgc gcgccgtggt cacaacgccc
     7501 ctgggcttgt tgagagcaag gtagatatgg tcagttgcag gatggaccgc ttggccgtcg
     7561 acggctacga catcgcgctc tgggtcaatg aaagtggcag gtgtagtcac caatacgcca
     7621 ttcaatgtta cgcgcccgga tgccacaagc tgttcgcacg cacgccgcga cgccaccccc
     7681 gaccgagcta ggaacttcgc gattcgttca gtcgtcatgc cttccgaaag cgctggagcc
     7741 ggtcgttgcg cccgatcacc accagcacgt cgtctgcgtg aatgacgtcg tcggcgccgg
     7801 gagctactac catcacatcg ccggacctaa ttgccatcac gtacacgcca aaacgggccc
     7861 gcaccgaaag ctccgcaagg gacttcccgg ccaggctcga cggggcagtc atctccacga
     7921 tgctgaagtc gggatcaagg tcgatctgct cgatgaggtt cgtggacgtg agcacgcgag
     7981 ccaaccgcga acctccatca cgctcagggt agacaactct gtcggcgcct agtttttcca
     8041 gaaccttgcc atgcaggtca tcgttagcct tggcgacgat ggtccgaaca cctagttccc
     8101 taaggaccat ggtccccacc atgctggcct tgacttcgct ccccacggtg atcactgcta
     8161 cgtccacgtt gcgaattcct atcgacctca tggcctcgat gtcggtgaca tcggccacca
     8221 ctgcgtgggt tacgaccggc gctattcgct gtaccttgtc ttcatccaca tcaatggcga
     8281 aaacctcctg gccaagctcg gacagggtgc gcgccacact catgccgaac cttccaagcc
     8341 cgatcactgc aaactgctgt gccatgtgcg ttcatcctct gttgccctcg cgcaaaacga
     8401 ggccgccgtt tggggctagc gccctaccgg attataccac aagcccaggc gctctaccag
     8461 tgccggactt gctttgggct gccgccgacc tctagagcaa cggaggcttt actgtgtcgg
     8521 gctgtgggcg aattgccccc atccgagtca gcacgtccgg tactacatcc agcaagcgat
     8581 cgaacacggc atccgtcgtg accggcagga acctgacctg cttgttgccg actaccagga
     8641 agcccactgg gttcagcgag atgccggcgc cgccgcctcc tccgaacggt gaagagttcg
     8701 aggcgggcgc cgattctgcc gcccagtcgg atccgccggc cgcgaacccg aaagtaacac
     8761 gtgaaacggg cataatcacg ttgccatcgg gggtctcgac agggtctccc agaatcgtgt
     8821 tgacatcgat catctggccg atgctgtcga gagtagtctt catcaatcca tctatgggat
     8881 ggtcgggcaa actctctctc ctccccagca tgcacgtggc gtgcaggcgc tgatcatcta
     8941 cactagtatt cgagaccgga ggacagttca ttcgctcgac gcttccgtag cgtcgctacg
     9001 agcgacagca gggcggcacg cagccgcgca tctagaagga gagtggcaag ggccgtctcc
     9061 acgactagat gaaatgaggt ggagaactgc gctttgccaa agacaggtgt gacagagaac
     9121 gacgggatct tgtgagccgg accccatctg gagcatgccc acgccgcaac gaccgacttg
     9181 acggcccaca gctcaccaca ggcgatggca gtggaagcag catcgcctgt gccgatagat
     9241 gaatgccatg acagctccgt cagcctatga cccagcgagg cggcagacgt gatcaccctg
     9301 gccattatgc gcagccgtac gaaggtctct gtaaggtccg agagcgtcca acgagcggtc
     9361 gggacggtgg gaggcggcac gtccctgcgg tagaaaggca cgaagatgaa gaaaggccat
     9421 ggccagacgc gaacggttgc ccgcgtacgc cccgagtcgc gcgcaattga tagatccagc
     9481 ctgaatggcc agtacagaac catggcgagc acgaccgcga gcgtcgcgcc cgccgtcagc
     9541 cccacttggg cccagcgcat caagaccact cctgtaggta ttgtgcgcgt tcactggtct
     9601 tctgattcgg gcagcggtgg caaatccgag agagaagtga gaccgagata cataaggaac
     9661 tccggtgtag tcccgtacaa aacaggcctg cccacgctgt tgcgcctgcc cacctcggcg
     9721 atgaggccac gctctgagag agtggcgatg ctcgagtcag acgacacgcc gcgaattcgc
     9781 tcgatttcgc ccctggtcac aggctggcgg taggctatga tagatagagt ctcgatagac
     9841 gccggcgata agggcgcagg acgctgcctt gccgacaggt tctccacaaa tggggcgtac
     9901 tccggacgac tcatcaagat gaatgagccg tctagcttcg aaaggacaat ccccgactcc
     9961 ggaaccgagt gcctgtctcc gagctcgcga aggagcttag ccgtcgtatc ttcgtctatc
    10021 tgaattgctg ccgctaagtc cttgacactc agggggaacg ctgcagcaaa caggatggcc
    10081 tcaattgccg cctgcgcttc atgtaagttc gtctgcggca cccccaatcg cctgtgatgt
    10141 cgcgaacatc cagatttctc cgaacggtgc aggttgtgat gccgagatgc gaccgcgcct
    10201 cactagttcc aggacggcca aaacggtgga gacgagcaga tggcgggatc tcgcgccgcc
    10261 aagaaactcg aacaatccaa gctttccctt gcgcgccagc cgtacaagca ccgaaaccat
    10321 ctgggtggct atgggtatct cgtcggcagg taggtacggg aactcctccg ggatacttga
    10381 cgaggcgtcc agatacgcct ggagcaggtc gtgcccggtg agcccctcca ccggattggc
    10441 ccgaacagtg ggtgtcggca gagggcgcag agggcgcgtg tacatacgag accccaccgc
    10501 ctccaagttc cggagcaccc gggccgcatc gcggtacctg cgatactcca ccagacgcgc
    10561 aacaagctca gcgcgggggt cggtcatctc agaagtggca gcggcgagct cttcgggaac
    10621 ctcctccggg ggcagcagag ccctcacctt aatgtatagc agagtcgccg caatgatcag
    10681 aaactcagct gcctgatcga tatcaagcca ttccatggca gatacctgtt cgaggtaatc
    10741 gtcggcgata tcgctgatcg atacttcgca tatgtcgatc tgcttcttct ctatcagcga
    10801 gagtagtaga tcgagcggcc ctgagaagcc ttcggtatct accttcatat cctcatcgcc
    10861 gagcgcactt cctccagcgt gcgggctgct ataggcctgg cgcgcatcgc gccgtcggcg
    10921 atgatcgact ctaccagacc gggagtggcc tcaagctgcc gcctccgctc gtgaatgggg
    10981 gccagaactg agatgagcgt atcggcgagt tgcctcttgc aggccacgca gccgatttct
    11041 gccccctcac attcgcccct gcgcccctct gccgactcgg gcgcgaacgc cgactggtat
    11101 gcaaacaccg tacacacatc cgggtgcccc gggtcgctct tgtgtatcct ggcgggatcg
    11161 gtgaccatgg actgcacggc ctggcgtatt ttctccggcg ggtcggcgat ctgaatggtg
    11221 ttgccgtagc tcttgctcat cttgcgaccg tccgtgcccg gtagcaccgc aactttgctc
    11281 aatttggcct gaggttctgg aaagaccggc gcatacatgt agttgaaccg gcgggcgatt
    11341 tccctggcga gctccaggtg gggcagctgg tcttcgccca caggcaccgt atcggccttg
    11401 tagactagta tgtcgctggc catcaacaca gggtagccca ggaatccgta ggtggttatc
    11461 tctcttccgg caagctgctg cagttgctcc ttataggtcg ggcatcgttc gagccagccc
    11521 aggggcgtta tcatcgacag caataagtgc agttccgcat gttccttcac gtcagactga
    11581 cggaagacga tggagcgctc gggatcgata cccgccgata tgaagtcgat gagcacttgc
    11641 cgggtatact cgcgaatcat cgacgtatcc tgatatcctg tggtgagagc atgccagtcg
    11701 accgctgcaa agaaacactc gtagtcgtcc tgcaggcgaa cccagttggc taaggctccg
    11761 gccaagttgc ccaggtgaag cattcctgtg ggcctcatcc cgctgaata
//
LOCUS       DAOTAC010000044        11749 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_4645_length_11749_cov_4.528391, whole genome shotgun sequence.
ACCESSION   DAOTAC010000044 DAOTAC010000000
VERSION     DAOTAC010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 11749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11749
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4645_length_11749_cov_4.528391"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..608
                     /locus_tag="PLM74_03150"
     CDS             126..608
                     /locus_tag="PLM74_03150"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HON42116.1"
                     /translation="MLQVDLHGMKTAEAMACFTRAYDSLLGAGARPGACLKVIHGYGS
                     GGAGGDTRRAVRSLLHSNPACADYTPGEDIDGSPGYTIVYPRRRLPSGAERLYPGIVE
                     YCAAPKTKLDVVRRFVRRSTESDIERALRELESGGRLRSFIKNGRKHYVDPHVVETRF
                     "
     gene            656..1042
                     /locus_tag="PLM74_03155"
     CDS             656..1042
                     /locus_tag="PLM74_03155"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HON42117.1"
                     /translation="MAEGLELRGVWARSGGYHFAYPDGRPWVGDMGPVQHLLAAVVGC
                     AGITMQAILSRMKIDHQGITIDAVATKADSTPTYVKSISLQATVHGAAADAAQLARVS
                     DLTEKYCLVAQTLRHDPDLTLSTITS"
     gene            1098..1367
                     /locus_tag="PLM74_03160"
     CDS             1098..1367
                     /locus_tag="PLM74_03160"
                     /inference="COORDINATES: protein motif:HMM:NF014624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HON42118.1"
                     /translation="MADESKQSVINRLKRVEGQVKGIQRMIDQGKRCSDILDQVAAAR
                     TALYNAGVLILEKHSRDCILGADDSDAVEEKVEELVKVIKKFARS"
     gene            complement(1614..2798)
                     /locus_tag="PLM74_03165"
     CDS             complement(1614..2798)
                     /locus_tag="PLM74_03165"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HON42119.1"
                     /translation="MSESRLKKYLPLIVVAIVSSIVTSIVVVSYVFPDRRGNLAAAVG
                     TAVPVSQVSQVDYKSIALPSFADVAEAVGPAVVRVDTERVEQFTTGDPRMDWMFRGLF
                     QGQQVVPGTGSGFIVSPDGHIVTNYHVIEGARKIEVTLTDGRKLRAQVIGGSKESDVA
                     VIKINAKNLPTAQLGDSGKLRPGDWVVAIGNPYGFDHTVTAGIVSALGRSIDDGDGNT
                     LATGDLIQTDAAINKGNSGGPLINMQGEVIGINTAIIQYAQGMGFAISMNSVRDVLNE
                     MMTRKVTEQSTSAPKPWLGIWYQQLDENLASYLQLPDTNGILITDVYKGSPADKAKLQ
                     KGDVVREVNRKPITKDLSLADEIGKMKIGDTVLLWIWRDGQRMYVSVKLAAYPDEEPE
                     LR"
     gene            complement(2791..3456)
                     /locus_tag="PLM74_03170"
     CDS             complement(2791..3456)
                     /locus_tag="PLM74_03170"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HON42120.1"
                     /translation="MTCNMTQRLISAYVDRELSEDERARVRQHLALCAECMAVYEMTL
                     EVKTALGSMAPSPCPECLWEGVRDRIAALPFQAEPERAPRLSPLRAVWSFARLVVPAA
                     VAGTIVAIPLVHMIFGVSVFGPAGPRQQPIASSTPPRLVITTPAAKESGVARYPATSP
                     VVSGQTASMSSSSLFGLDTTIRRGDNNDEHSSSPDEQLNLLLRSMQDSSFTYTWIGGS
                     THE"
     gene            complement(3453..4058)
                     /locus_tag="PLM74_03175"
     CDS             complement(3453..4058)
                     /locus_tag="PLM74_03175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HON42121.1"
                     /translation="MVNQQALATTAQHLSREAEFESLVLRYQNSVYNIALRMSGNPDE
                     AQDLAQEAFVRAYRSFDSYRRGTSFDKWLYKIVTNLYIDEVRRRRRRPYVESLDERIT
                     MSGGDEIEREIADVSHSPETVYDREHLDMCIQSALAELPPEFRMAVILCDIEGFSYEE
                     IAQIMGTSIGTVRSRIHRGRRGLRERIAPCFPARSGEVRPT"
     gene            4242..4949
                     /locus_tag="PLM74_03180"
                     /pseudo
     CDS             4242..4949
                     /locus_tag="PLM74_03180"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
     gene            5580..6818
                     /locus_tag="PLM74_03185"
     CDS             5580..6818
                     /locus_tag="PLM74_03185"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR00045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HON42122.1"
                     /translation="MVKQPKVVIAPDSFKGSLTARQVCEAAEEGLRRVWPEAQVVSVP
                     MADGGEGTVQSLVDATSGRIVNIDVTGPLGEPVAAFFGVLGDGVTAVIEMAAASGLPL
                     VPQGLRNPLSATTRGTGELIESALDMGCRKFIIGIGGSATNDGGAGMAQALGVRLLDA
                     HGVEIRPGGAELARLTRIDASGLDPRASQSEFVVACDVDNPLTGPRGASAVYGPQKGA
                     TPEMVKILDDALKNLAAVVRRDLGVDVDGIPGAGAAGGLGAGLMAFLGASLKRGIDIV
                     VETVRLREQMKGAALVITGEGRTDFQTLFGKTPMGVANVAKSLGIPVVVISGSIADDA
                     GGLYAHGIDALMSIAKGPCTIDEAIAGAGPLMADAAETAARLIAVGLGASLGPGASLG
                     REAGPGRETREGSGLLGGLE"
     gene            6818..8305
                     /gene="murD"
                     /locus_tag="PLM74_03190"
     CDS             6818..8305
                     /gene="murD"
                     /locus_tag="PLM74_03190"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HON42123.1"
                     /translation="MLTDAQAGVLRGLAGKRAAIVGIGVTNVPLIRFLAGQGVQVTAC
                     DQKQENSLASELTSLAGLDVQYLLGPGYLDALPGFDIVYLSPGVNPGQPEIEAARRAG
                     VAISSEIDLVLRLSAAPTVGITGSSGKTTTTTLTGLILREDVRRHHPGRQVLVGGNIG
                     APVIERVLTVGPDDLIVLELSSFQLKLLEVSPRVAAVLNVTPNHLDVHPSMEDYIWSK
                     ENIVRYQRDDDWAVFGADNDVTQLMAERAPGQIMFFSVSRPVPRGAFLDGDRLMMRWS
                     EGAGSGDLGGSGGSGTGDTVVARRSDLKIPGMHNVQNVLAAVAVCAALGAEPGSMRDA
                     IAAFSGVEHRLEPAGEIDGVVFINDSIATTPSRTIAALTAMERPIVLIAGGYDKHLPF
                     DEMARLALGRVHTVALVGVTADKIAQAFEEAAGKAGVEPPSIIRADTFREAVGAARLA
                     ARSGDAVLLSPACASYGLFRNFEERGRAFKQLVSEFAAEAGKAGE"
     gene            8378..9598
                     /locus_tag="PLM74_03195"
     CDS             8378..9598
                     /locus_tag="PLM74_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42124.1"
                     /translation="MQGSIGRRGRKHAKVRRIRAHHSSLPRAASAAVLFAICTMVLAV
                     NAVWWKTERGYPMGMSLFDALPGLGRASAGAATAPNASDGKTGLMLSVDIDITRDPGS
                     EVASSRWTSSQDGVIELYARDEASAGSGGRSGYRMLDRVEAGPVVELSCATLSQQPEA
                     GSSAAQLERPMWGTLLVAAAEYDQSVGAMSKSKVTTAYAWNGRNLVEVWSATTRSEAV
                     WNLAWDAPKETEAGRAHAGSGSGSSSSSGPGLGAGAGTGAGAGSADWMRLTSLSKVDF
                     VASSQAGSRPSIVLRSSHKHESYDENIDVFTTIKSREVVQTYYWSDKWSAFILSEAIV
                     GHSGASGMEYPGAVSRFSIPAGTALAVLESERQCAEGLVFDPPHLLRVKMPDGRRCFV
                     DDGSVVPTPSRAMV"
     gene            9691..10545
                     /gene="folD"
                     /locus_tag="PLM74_03200"
     CDS             9691..10545
                     /gene="folD"
                     /locus_tag="PLM74_03200"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HON42125.1"
                     /translation="MPATIIDGKAVAASIRQDAAARAARFKEEYGRPPGLAVVIVGDN
                     PASRVYVNSKKKACAECGIYSEEHALPADTAEDDLLDLVRHLNSDPNIDGILVQLPLP
                     DHISEDKVIETITPAKDVDGFHPINVGNLVVGKPALRPCTPAGIMELIRRTGVELKGK
                     HAVVVGRSNIVGKPVALMLLAEHATVTICHSRTVDLPSVCRTADVLVAAVGRPEMIKG
                     DWVKPGAVVIDVGVNRIAEKKLVGDVDFDGASAVAAAITPVPGGVGPMTIAMLMQNCV
                     EAAFAAQR"
     gene            10567..11466
                     /locus_tag="PLM74_03205"
     CDS             10567..11466
                     /locus_tag="PLM74_03205"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HON42126.1"
                     /translation="MDYEIYDVMIIGAGPAGMSAAVNARVRNKSTVLISRRFASPSLA
                     KAPSVHNYLGVEELSGSELYSRFLEHARRAEARLIEADVTGIYDMGGHFAALTSAGDI
                     SARAVIVATGAVQSASIPGESALLGAGVSYCATCDGPLYRGKRVVVLGYTPEAREEAN
                     FLAEICSEVTYVDLQRGKAEKGVPLAESIREVVAKVAGIRGESFVTHVELEGEAIPTD
                     GVFVIRESIPAERLLDGLAVTEGAIVVDREMATSVPGVFAAGDCTGRPWQVAKAVGEG
                     LVAGLSASRYVDRAMAAARAEGR"
     gene            11471..>11749
                     /locus_tag="PLM74_03210"
     CDS             11471..>11749
                     /locus_tag="PLM74_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HON42127.1"
                     /translation="MNQHRRPELAACTAPIMPGAEPYSMGPADAQVACLVVHGFTGSP
                     YEMRPFAERLAARGVYADVPLLPGHGTSVEDMSRTSFPDWVAGAEAAYL"
BASE COUNT         2247 a         3359 c         3798 g         2345 t
ORIGIN      
        1 taggcaatga gagcgatacg ggtagggggt gtcgtcgccg cagggctcat ggttcctgag
       61 gtgtactgtg tcaccccttt tcgctacatc ttttttcatg gtatttgtcg ggcggggcgg
      121 ttaatgtgct acaggtagat cttcacggca tgaagacggc agaagccatg gcttgcttca
      181 caagggcata cgacagcctc cttggcgcag gcgcgcgccc cggcgcatgc ctcaaggtga
      241 tccatggcta cggatcggga ggagcgggcg gcgatacgcg tcgcgcagtg cgctcactgc
      301 ttcactccaa cccggcctgt gccgattaca ctcctgggga agacatcgac ggcagcccgg
      361 ggtacaccat agtctacccg cgacggcgtc tgccgtccgg cgcggaaagg ctttacccgg
      421 ggattgtaga gtactgcgcg gcgcccaaaa ctaagctcga cgttgtccgc cggtttgtgc
      481 ggagaagcac tgaaagcgat atcgaacgtg ccctacgtga actagaaagt ggcggcaggt
      541 tgcgatcgtt catcaagaat gggcgtaagc attatgtgga cccgcacgtt gtagaaacga
      601 gattctagat gcgagtttga cgcatacggg tctttgtgaa agggggaaca atctattggc
      661 tgaagggctt gagctcaggg gcgtgtgggc tcgatcaggc ggttatcact tcgcataccc
      721 ggacggacgg ccgtgggtag gcgatatggg tcccgtgcag cacctgcttg cagccgtcgt
      781 agggtgcgcg ggcatcacaa tgcaggctat tctgagcaga atgaagattg atcaccaggg
      841 aatcaccata gacgcggttg ccacaaaagc cgatagtacc cccacgtacg tgaagtcgat
      901 ttcgctgcaa gccacggtgc atggcgctgc agcggacgct gctcagttgg cacgggtgtc
      961 tgatctcacc gagaaatact gccttgtggc ccagacgctc aggcatgacc ccgacttgac
     1021 attgtccacc ataaccagtt gattgcctgg gttgaccaca tgggctgacc acctggagag
     1081 aaagggagtg tgcgttcgtg gcggatgaga gcaagcaaag cgtgatcaac aggctcaagc
     1141 gcgtagaagg tcaagtaaag ggcatacaac gcatgattga ccagggcaag agatgctccg
     1201 acattctaga tcaggtggcc gcggcacgga ccgctctcta caatgcgggc gttctgattc
     1261 tggaaaagca ttctcgcgat tgcattctcg gcgctgatga ttccgacgca gtcgaagaga
     1321 aggttgagga actggtgaag gtcatcaaga agttcgcccg gtcgtgagat ctagccccag
     1381 aaacggagct ggagcgagca ggtgcgtgag agaatcgtcc ccatggcaac aaccgcaacg
     1441 tcacgtctta tcctgctcgc cgtggatagt gtgtgcactc ttgctccgga tattcagcga
     1501 gccgggcggc ccaatacagg aataatttgg ggtccagcct ccggatcaga cgctccggat
     1561 cagatggctg gaccccttgg ctaggcccgt ttctcacagt ccatcaggag cggctagcgg
     1621 agctccggct cttcatcagg gtaggcggca agcttcaccg atacatacat cctctggccg
     1681 tctctccaaa tccacaggag tacggtgtcg ccaatcttca tcttgccaat ctcatcggcg
     1741 agtgatagat ccttcgttat cggcttccgg ttgacttcgc gcacaacgtc gcccttctga
     1801 agtttggcct tatctgcggg actgcctttg tagacgtcgg taatcagaat gccgtttgtg
     1861 tcgggcaact gcaagtagct ggcaaggttc tcgtccaact gctgatacca gatgccgagc
     1921 cacggcttag gagcacttgt ggattgctca gtcactttgc gcgtcatcat ctcgttcagt
     1981 acgtctctca cggagttcat ggagatcgca aaccccatgc cctgcgcata ctggatgatg
     2041 gcggtgttaa tcccgatcac ttcaccctgc atgttgatga ggggcccgcc gctattgccc
     2101 ttgttgattg ctgcgtcggt ctgtatcagg tcgcccgtag ccagggtgtt cccgtcgcca
     2161 tcgtctatgc tcctgcccag cgcactcacg attccggctg tgaccgtgtg gtcgaacccg
     2221 tagggattac ctatggctac tacccagtcg cccggcctga gcttgcctga atcgccgagc
     2281 tgtgctgtgg ggagattctt ggcgttgatc ttaatcacgg caacgtccga ttccttgcta
     2341 ccgccgatta cctgggcacg aagtttccgc ccatccgtca gcgtgacctc gattttccgc
     2401 gccccttcga tcacatggta gttcgtaaca atgtgcccgt ccgggctgac aatgaaacct
     2461 gagccggtgc ccggcactac ctgctggccc tggaaaaggc cccggaacat ccagtccatg
     2521 cgaggatctc ctgtcgtgaa ttgctccacg cgttcagtgt caactcggac taccgccggc
     2581 cccactgcct ccgctacgtc agcgaaggac gggagtgcta tggacttgta gtctacttgc
     2641 gatacctgcg ataccggcac tgcggttccc acagccgcag ccagattgcc tctgcggtct
     2701 gggaacacgt acgacactac tacgatagag gtgactatgc tgcttactat ggccaccact
     2761 atcagcggaa ggtacttctt aagcctggat tcactcatgc gtgctccctc caatccatgt
     2821 ataggtgaaa ctcgaatcct gcatgctcct gagcagcagg ttcagctgct cgtccggcga
     2881 actggaatgt tcatcattat tgtcgccgcg gcggatcgtc gtgtccaggc cgaacaggct
     2941 cgatgacgac atgctagctg tctgcccgga gacaactggg ctcgttgccg ggtatcgagc
     3001 gacccccgac tctttggccg ccggcgtggt tatcacgagt cgtgggggag tggaggacgc
     3061 tatgggttgc tgccgcgggc cggccggacc gaatacgctc accccgaaga tcatatgcac
     3121 cagcgggatg gcgacgattg tgcctgctac agccgctggc acgactagcc ttgcgaatga
     3181 ccagactgcc ctgagcggac tcagccgcgg cgcgcgttcc ggttcagcct gaaagggcaa
     3241 cgccgctatt cggtctcgga cgccttccca caggcactcg gggcatggcg aaggcgccat
     3301 cgagcccaga gccgtcttca cttcaagagt catctcgtac acggccatgc actctgcgca
     3361 cagggccaga tgctgtcgca ctcttgccct ttcatcctcc gacagctcgc gatcgacata
     3421 ggccgatatc agccgctggg tcatgttgca cgtcatgtcg gcctcacctc ccctgaccgc
     3481 gccggaaagc agggcgcaat gcgctcgcgc agccctcgtc ttcccctgtg aatgcgcgac
     3541 ctcactgttc cgatcgacgt gcccattatc tgagctatct cttcgtatga aaacccttcg
     3601 atatcacaca gtataaccgc catgcgaaac tccggcggca gctcggccaa tgcactttgg
     3661 atgcacatgt ccaggtgctc ccggtcatac accgtctccg ggctgtgcga aacgtcggcg
     3721 atctcgcgtt cgatctcgtc gcctcccgac attgttatgc gctcatctag cgactcgacg
     3781 tacggcctgc gcctgcgcct gcggacttcg tcgatgtaga ggttggttac gattttgtag
     3841 agccacttgt cgaaggaggt gcctcgccgg tagctgtcga acgacctgta tgcccttacg
     3901 aaagcctcct gtgcgagatc ctgggcctcg tcggggttgc ccgacatacg aagcgcgatg
     3961 ttgtatactg agttctgata gcgaagtacg agcgattcga actccgcttc cctcgacagg
     4021 tgctgtgcag ttgtggctag tgcctgctgg ttgaccaaga gcatcacccg cccagacgtg
     4081 atctgcgcct aactgtccgt ttcacttggg taacaacgtc atgcgcgagg cgcgagttcc
     4141 cgcttcgatt ataccttccc acaggcagat gggcgagggc ggcttaggcc ctgaatggga
     4201 actcgctgga aaccaaaggc atgaacaagg aggtggaggg gatgtcagcc ggagcaccgg
     4261 atctgcgctc tttcatccct ccggctcaag tcatcgaagt cattgctgct ctgagggagc
     4321 tgggcaatac ggcgtacatt gcaggcggcg ccgtgagaga catggtgatg ggcaggccca
     4381 gaccggagga ttgggacgta gcgacctccg ccagtcctga gcaggtgatg gctgcgtttg
     4441 accgggtgat cccgagtggc gtggctcacg gcacggtgac agtgctggac aacggcatga
     4501 gcatcgaggt gaccacatac cgcgtagacg ggccgtatct ggacggtagg catccggagt
     4561 acgtgcggtt tgttccgcgc attgaggatg acctcgcccg acgggacttc accatgaacg
     4621 ccatggctta cgatcccatc gccggagagc tgatagaccc atacggtggg cgcgaggcca
     4681 tacgagcacg tgtcattact tgcgtgggcg agcccagaga caggttctca gaagacaagc
     4741 tccggatggt gcgtgccgct cgctttgctg cgaaactcag gtttagggcc gacccgcgtc
     4801 tcgtggaggc cgctcgtgaa ttggcgcccc agatacggca ggtatcggtg gagcgcgtat
     4861 acgacgaact cgccaagatg atggggacgc gcgcaccttc gctcggcttc gagctgctcg
     4921 aggagatggg gatcaccccg tacgtcctgc ctgagctgtg cgccgacgtg ccacgcgagc
     4981 ggagggcccg tctgtacgct atgtgcgacg aattgccgcc tgacctggta gaacgcttcg
     5041 ccgtgcttct tgcccctctc ggcgagagtc gtgcccggca gacgctgtcc cgaatgcgtg
     5101 cgtccaatcg caccggagcc gacgtggttg ccatagtccg gggcgcgcag gcagtgctga
     5161 actcgcattc ggctgcggat gcgaatgcgg attcggatcc ggccggcctt gccggcctgt
     5221 cttcctactc tttgagactg gaagcatcgc ggatcggcgt gggcaacctg gcgccggctg
     5281 tgcgggtggc atgcatcttt gcagggtgct ccgcagatga aagacgtgcc ctggatgagc
     5341 tagtccgctc cgttgttcag cagaagcctg ccctgcgcat ccgggacttg gctgtggacg
     5401 ggcatgacgt gatgaacgca acgggcattg gcccaggccc tggcgtgaga ctcgttcttt
     5461 cggcgctcct cgatgaggtt ctgcgcgatc ctgccctgaa caccagagag cgcctgctca
     5521 agctggctcg caaccgctat caatcgctcc attcagttcc gcgttctggg ggtgttttca
     5581 tggtcaagca accgaaagta gtaatagccc ctgactcgtt caaaggcagc ctcactgccc
     5641 ggcaggtgtg cgaggccgca gaagaagggc tgcgcagggt gtggcccgag gcccaagtag
     5701 tgtcggtccc aatggccgac ggcggcgaag gcacggtaca gtcgctggtc gatgcaactt
     5761 ccgggcggat agtcaatatc gacgttacag gtccgctggg cgaaccggtc gccgcattct
     5821 tcggagtgct cggcgacggc gttacagctg tcatagagat ggccgcagcg tctgggttgc
     5881 cgctggttcc ccagggcctc cgaaacccgc ttagcgccac aactcggggc acaggcgagc
     5941 ttatcgaaag cgctctcgac atgggttgcc gcaagttcat aatcggcatc ggcggctccg
     6001 ccactaacga tggaggcgcc ggcatggcgc aggcgctcgg cgtgaggctt cttgacgcgc
     6061 atggtgtcga gatccgaccg ggcggcgccg agcttgcacg cttgacgcga atcgatgcgt
     6121 cgggtctgga cccgcgggcg tcgcagtcgg aattcgtggt tgcatgcgat gttgacaacc
     6181 ctctcaccgg cccgcgcggc gcgtcggctg tgtatggccc gcagaagggc gcgacgcccg
     6241 agatggtcaa gatacttgac gacgccctga agaacctggc ggcagttgtc aggcgcgacc
     6301 tgggagtgga cgtggacggt atccctggcg ccggtgccgc aggggggctg ggcgcaggcc
     6361 tgatggcatt cctcggcgcc agcctcaaac gaggaataga cattgttgta gagactgtgc
     6421 ggctcaggga gcagatgaag ggagcggccc tggtcatcac gggcgaagga cgcaccgact
     6481 tccagaccct cttcggcaag acccccatgg gagtggcgaa cgtggccaaa tcgctgggaa
     6541 tcccggtcgt ggtcatatcc ggctcgatcg cagatgatgc cgggggtctg tacgcgcacg
     6601 gcatcgacgc actgatgagt atcgcaaaag gtccatgcac aatcgacgag gcaattgcgg
     6661 gcgccggccc gctcatggcc gatgctgcag aaacggcggc gcgcctcatt gccgttggtc
     6721 tcggcgcgag ccttggccct ggtgctagcc taggccgcga ggctggccct ggccgcgaga
     6781 ctcgcgaggg cagcggtctt ttgggggggc ttgagtgatg ctgaccgacg ctcaagcggg
     6841 cgttctccgg gggctcgcag gcaaacgcgc agccatagtg ggcataggcg tgactaatgt
     6901 tccgctgatt cggtttctgg cgggtcaagg ggtgcaggtt accgcatgcg atcagaaaca
     6961 ggagaattcc ctcgcctctg agctgacctc ccttgccggg ctagatgttc aataccttct
     7021 ggggccaggc taccttgatg cgctgccggg cttcgacatc gtgtatcttt caccgggagt
     7081 caatccaggg cagcccgaaa tcgaggccgc acgccgcgcc ggcgtggcca tcagctccga
     7141 aatcgacctc gtgcttaggc tatcggccgc cccgactgtg gggattacag gcagttcagg
     7201 caagaccacc actactactc tcacgggact catcctgcgg gaagacgtga ggcgccatca
     7261 ccccggtcgc caggtgttgg tgggcggcaa cataggcgct cccgtcatcg aacgcgtgct
     7321 cactgtgggt cctgacgacc tgatagtgct ggagctatcc agtttccaac tgaagttgct
     7381 tgaagtcagc ccgcgtgttg ccgcggtgct caacgtcacc ccgaatcacc tcgacgtgca
     7441 tccgagcatg gaagactaca tctggtcaaa ggagaacatc gtacgttacc aaagggacga
     7501 cgattgggca gtattcggtg ccgacaacga tgtcacgcag ttgatggctg agcgggcgcc
     7561 tggccagatc atgttcttca gtgtgtcgcg accggtgcct cgcggggcct ttctcgacgg
     7621 ggacaggctg atgatgagat ggtctgaggg agccggatca ggcgacttag gcggatcagg
     7681 tggatcgggc acgggcgaca cggttgtcgc gcgccgctct gacctcaaaa taccggggat
     7741 gcacaacgtc cagaacgtgc tggcagcagt tgctgtatgc gccgcgctag gcgcagagcc
     7801 gggctctatg cgcgatgcga tagcagcatt ttcaggagtg gagcaccgcc tggaaccggc
     7861 tggcgaaatc gacggcgtgg tcttcatcaa cgactcgata gcgactactc cgtcccggac
     7921 gattgccgct cttacggcaa tggagcgtcc tatcgtgctc attgcaggtg gttacgacaa
     7981 gcatcttcct ttcgacgaaa tggctcggct ggcgttgggc agagtgcata ctgtggcgct
     8041 tgtgggcgtg accgccgaca agattgcaca ggcatttgag gaagcagctg gtaaagcggg
     8101 ggtcgaaccg cctagcatca ttagggcgga cacattccgc gaggctgtgg gagcggcgcg
     8161 actcgcggcg cggtcgggcg atgctgtact gctctcgccg gcatgcgcca gctacgggct
     8221 gttccgcaat ttcgaggaga gggggcgcgc attcaagcaa ctggtgtcgg agtttgcggc
     8281 ggaggcgggc aaagctggtg agtgatgacc cgaatccgta atcgtaggcc ccctgcacga
     8341 gtaggattta accagtctcg tgaggggggt tgcttttatg caaggcagta ttggcagacg
     8401 cggtcgaaaa cacgccaaag tcaggaggat ccgtgcccac cacagtagct tgccgcgggc
     8461 ggctagcgcc gctgtgctgt ttgccatatg tacgatggtg ctggcagtga atgccgtctg
     8521 gtggaagacg gagcgagggt accccatggg catgtcgctg tttgacgcct taccgggtct
     8581 cggacgcgcc tcggcgggcg cggcgacggc gccgaatgca agcgacggca agaccggcct
     8641 catgctcagc gtggacatcg acataacgcg ggatcctggc tccgaagtgg ccagctcgcg
     8701 atggacatcg tcgcaggacg gtgtgataga actctacgcg cgcgacgaag cttctgccgg
     8761 aagcggcgga cggtcgggat accgcatgct cgaccgcgtg gaggccggcc cggttgtgga
     8821 gctatcctgt gcgacgctgt cacagcagcc agaggccgga tcatccgctg cgcagcttga
     8881 gcggcctatg tggggcacgc tgctggtggc tgcggccgag tacgatcaga gtgtgggagc
     8941 catgagcaaa tcgaaagtca ctacagcgta tgcttggaac ggccgcaatc tggtcgaagt
     9001 ctggtcggcc acgacgcgct cggaagctgt atggaacctc gcgtgggatg cgccgaagga
     9061 gaccgaggct ggtcgtgcgc acgcaggctc gggctccggc tcgagctcga gctcgggccc
     9121 gggcttgggc gccggggctg gtactggtgc tggtgcgggc agcgcggatt ggatgcgttt
     9181 gaccagtctt tcgaaggtcg attttgtcgc atcgtcgcag gcagggtcgc ggccttccat
     9241 agtgctgcgg tcgtcgcaca aacatgagtc atacgacgag aacatcgatg tgttcactac
     9301 catcaaatcc cgcgaggttg tgcagactta ctattggagc gacaagtgga gtgcgttcat
     9361 cctgtctgag gcgatagtag gccacagcgg ggcgtctggg atggagtatc cgggagctgt
     9421 gagtcggttc agtatcccgg ctggaacagc cttggcagtg ctggagtcgg agaggcagtg
     9481 cgcggaagga cttgtgttcg acccacccca cctgctcagg gtgaagatgc ctgacggcag
     9541 gcggtgcttt gtggacgacg gcagtgttgt cccaacgcct tcgcgtgcca tggtataatg
     9601 caactgtccg gccaatgccc gccggcagtg gcaccgctgt ccggccagcg gggggctgat
     9661 ggctgcatta ctgctgaatg gagtgattct atgccggcta ctataatcga cggcaaggct
     9721 gtagctgcgt caatccgaca ggacgcagcg gctcgggctg ctcgcttcaa ggaagagtac
     9781 ggacgtcctc cggggctcgc agtcgtcatt gtgggcgaca acccagcatc acgcgtttat
     9841 gtcaacagca agaagaaggc ctgcgcggag tgtgggatct actctgaaga acatgcgctt
     9901 ccggctgaca ctgccgagga cgatctgctg gacttagtgc gccacctcaa ctctgacccc
     9961 aacatcgacg ggattctggt tcagctcccg cttcccgatc acatctctga agacaaagtc
    10021 atcgagacaa tcacgccggc taaagatgtc gatgggtttc acccgatcaa cgtggggaat
    10081 ctggtcgtgg gcaagccggc gctcaggcca tgcacgcccg cagggatcat ggagctgata
    10141 cgccgcaccg gagtagaact caagggtaag cacgcggtgg tagttggcag gagcaacata
    10201 gtaggcaagc ccgtcgcgct gatgctcttg gctgaacacg ccacagtcac catctgccac
    10261 tcgcgcaccg tcgatctacc atccgtgtgt cgcacagcag atgtgctcgt ggcggcggta
    10321 ggcaggccgg agatgataaa gggagactgg gtcaagcccg gcgcggtcgt gatagacgtg
    10381 ggcgtcaaca ggatagccga gaagaagctc gtgggcgatg tggacttcga cggagcgagc
    10441 gcggttgccg ccgccataac cccggtgcca ggcggtgtcg ggcccatgac catcgccatg
    10501 ctgatgcaga actgcgtgga agcggccttt gcagcccaga ggtagcaact ggggagttga
    10561 ttgacagtgg actacgagat ctacgacgtg atgatcatcg gagctggccc cgccggcatg
    10621 tcggcggctg tgaatgcgcg cgttcgcaac aagtcgacgg tcctcatttc ccgcaggttc
    10681 gccagcccga gccttgcgaa agcgccgtca gtccataact acctaggcgt ggaggagttg
    10741 tcgggatccg agctatactc gcgctttctg gagcatgcgc gtagggcgga agcacggctg
    10801 atcgaagccg acgtgacagg tatctacgac atgggtggcc acttcgcggc gctcacctcg
    10861 gccggcgata tctcggcgcg tgccgtgata gtcgccacgg gggccgtgca gtcggcgtcg
    10921 attccgggcg aatcggcgct gctcggtgca ggagtcagtt actgcgctac ctgcgacggc
    10981 cctctttacc ggggaaagcg tgtggtggtg ctgggataca cacccgaggc gcgcgaagag
    11041 gccaacttcc tcgccgagat atgctcggaa gttacatacg tcgacttgca gcggggcaag
    11101 gccgagaagg gcgtgccgct ggcggagtcg attcgtgaag ttgtcgcgaa ggtcgcgggc
    11161 atacgaggcg agagcttcgt tacccatgtt gagctggagg gcgaggcaat ccctacggac
    11221 ggggtattcg tgatacgcga gtcaatcccg gcggagcgtc tcctggatgg tctggctgtg
    11281 accgagggcg ccatcgtagt tgatcgagag atggcgacaa gcgtacctgg ggtgtttgcc
    11341 gcgggcgact gtaccgggcg accatggcag gtagcgaagg ccgtaggtga aggattggtg
    11401 gctgggttat cggcatcccg atatgtcgac agggccatgg cggcagcgcg ggccgaaggc
    11461 cggtgagacc atgaatcagc atcgaaggcc tgaacttgcg gcgtgcactg cgccgataat
    11521 gcctggtgct gagccctact cgatgggccc agctgatgca caggttgcct gcctcgtggt
    11581 gcacgggttt accggatcgc catacgagat gaggccgttc gcggagagac tggcggctcg
    11641 gggcgtgtac gcggacgtgc cgctcctccc agggcatggc acgtcagtgg aggacatgag
    11701 ccgcacatcc tttccggact gggtggcagg agctgaagca gcctatcta
//
LOCUS       DAOTAC010000045        11691 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_4673_length_11691_cov_4.452733, whole genome shotgun sequence.
ACCESSION   DAOTAC010000045 DAOTAC010000000
VERSION     DAOTAC010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 11691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11691
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4673_length_11691_cov_4.452733"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            220..699
                     /gene="smpB"
                     /locus_tag="PLM74_03215"
     CDS             220..699
                     /gene="smpB"
                     /locus_tag="PLM74_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HON42128.1"
                     /translation="MSNEKTHEKLVATNRKARHEYFIEETYEAGIALTGTEIQSVRQG
                     RVSLGDAYAEVRGSEVFLVNSHIAEYDYGNRFNHNPRRPRKLLLHKSEIRRLDSRVRE
                     RGYTIVPLRMYLRGGLAKLEIGLARGKKTWDKREDIAKRNVQREIERTAKASGRERE"
     gene            780..1133
                     /gene="ssrA"
                     /locus_tag="PLM74_03220"
     tmRNA           780..1133
                     /gene="ssrA"
                     /locus_tag="PLM74_03220"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            1392..1952
                     /locus_tag="PLM74_03225"
     CDS             1392..1952
                     /locus_tag="PLM74_03225"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HON42129.1"
                     /translation="MAEKDIRERIKEVAVEHFNTSGYHGTTIRNIAKDVNCSLPMIYY
                     YFNSKKELFDEIIKKEYFEVLKRQAALSNTSNIVDFYTKFVYNLNFLSNYDKQVYRLG
                     IKVYLSFDGDQELMEIMDEWEKTILPRHYQILEPHLKDVDNGIAIVRTLVHLLETLIE
                     SIVVKNRFLPEEEVREEISIVLRGCE"
     gene            2040..2591
                     /locus_tag="PLM74_03230"
     CDS             2040..2591
                     /locus_tag="PLM74_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42130.1"
                     /translation="MDIETRLALLQNTYAASIAETVNTYDKLKVLDTIVERRKERQAQ
                     TAPFLNQQLGIETVEDVFGKLSEIYGCANWSVETTDKGYVATAVSCKLCALSKKMGGA
                     NPCNGWCLDPMSAMITAAGKIDAESIIVESTLMVGDCCKVLINISAEQDDTAETSLTY
                     GDDGPNTSLPSRRITSGCSILLC"
     gene            2686..3636
                     /gene="rnhC"
                     /locus_tag="PLM74_03235"
     CDS             2686..3636
                     /gene="rnhC"
                     /locus_tag="PLM74_03235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HON42131.1"
                     /translation="MKRRWFELSEKDLSGDDMARLARFVAERGWEVCQERAIQHGRQM
                     KISDGQNQATVVVYFTGKSLVQGRSSSLTDELAEWCSTSLRGSVSRTEAHGGGRLPNP
                     AHACGIPRIGIDESGKGDYFGPMVAAGVYVDRGSELVLVKMGVVDSKRLSDARIAKLA
                     QAIRSECPHSVVVIGPARYNQLYVSMRNVNVMLAWAHSRCLENVLTRVECGLAVSDQF
                     GDESYLTQALMERGRKIKLEQHPRAEADVAVAAASILARDEFVRRLSALSQQAGIALP
                     KGASDPRIVAVASEIASKGGREALADYAKLHFRTTESLGI"
     gene            3666..4187
                     /locus_tag="PLM74_03240"
     CDS             3666..4187
                     /locus_tag="PLM74_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42132.1"
                     /translation="MQMDGENGSSENRRRHWAGIVLIIVGCYLLASQFVSGRIMGMLA
                     APGIGALLLLWGAVSRSVGLIVVGGVVFGSGAGSFLASEVFWRLKGPANAGVIVLSMA
                     AGWFMIMLLSERFAGNPQRWALVPAVIMTAVGVPLILSSVAPVVLEWVVTWWPALLII
                     AGLLLFLRRGGGG"
     gene            4286..5464
                     /locus_tag="PLM74_03245"
     CDS             4286..5464
                     /locus_tag="PLM74_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HON42133.1"
                     /translation="MRARQIREGIFWMGAVDWDRRLFDSLIPLPDGTSYNAYLVRGSS
                     KTALLDTVDPSVYYQLDAQLASVEHLDYVVAHHGEQDHSGSIPAVLARYPEASVVCSP
                     KCKPMLIDLLHVAEERIVVVDDGDTIDLGGRTLKFVHAPWVHWPETMLTYVPEDKILF
                     TCDFLGSHLATSELYSTGRPREREAAKRYFAEIMMPFSNLIVKNLDKIDGLDVDVVAP
                     SHGPVVSDPGYITGCYREWSAGPMRNKVCLPYVSMHGSTRVMVDALVSALVGRGVAVE
                     RFELTGSDLGNVAMSLVDAATVVIGTPTVLGGAHPAAAHVALLANALRPRTKFVSVMC
                     SYSWGGRAVEQISGLIGNLRAEVLEPVVCKGLPRSQDLQAIERLADQIAQKHRELGLA
                     "
     gene            5999..6949
                     /locus_tag="PLM74_03250"
     CDS             5999..6949
                     /locus_tag="PLM74_03250"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019534167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HON42134.1"
                     /translation="MDVTTRERYLGSMIGLAVGDAVGAQVEFMRPGDFEPVVDMVGGG
                     VFGLPAGAFTDDTSMALCLAQSLVERGGFDPVDQLERYLRWMREGYMSSIGRAFDVGG
                     TCADAIERFEATRRPYCGSTNPNRAGNGSIMRLAPVPLFFAHDPEKAVEMSAASSRTT
                     HGARNAVDGCRYLGALIVGAVGGASKEQLLSPRYTPAPGLWERAPLADAIREVADGSF
                     VMKNPPEIRGTGYVVDSLEAALWAFHHGHSFEDGLMLAVNLGDDADTTAAVYGQLAGA
                     FYGVSAIPQRFRDRLVMRDLIEELAEKLYQCAFEATHGGS"
     gene            7391..8431
                     /locus_tag="PLM74_03255"
     CDS             7391..8431
                     /locus_tag="PLM74_03255"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HON42135.1"
                     /translation="MSTTLQDIADMLKLSKSTVSRALSGDPRVAEDTRARVASLARHM
                     GYTPNPTARALATRRTNTIGLAVPWAPRSLSDPFYLEFLGAAGDEAMRSGYSLFLSTP
                     DGDGAGAVRAHAELADPRRIDGMILTEPRANDERIALLQSVGLPFVVLGVVPDPLVSW
                     ISGDNTTGARDAVDYMISLGHTRIACITGPPDQTASDARFEGYRLAMSGAGLPLDRNI
                     IEAGDFTQSGGYSAMHAIIGSRGVPSAVFACNDVMALGAMRALREAGLAVPSDVSVVG
                     FDGISMAEYVDPPLATVKQPIQELGRMAVQILIGRMSGEGGPVRCILPVQFQPRDSVG
                     SPRLGANAADRG"
     gene            8518..9708
                     /locus_tag="PLM74_03260"
     CDS             8518..9708
                     /locus_tag="PLM74_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013933060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HON42136.1"
                     /translation="MMRRKVTVLLLIVLAVFSLTGLAAEKKLTVISGWSGPEMDAFMP
                     VLREFEKETGIRVDYQIYRAEDLAVLLPAQFDSKTAPADVIFMWAWFINKQAQSGHVL
                     DMTGVLNESDFVPGALDAVKSGAKLWGAPYTGKIKPGFWYRKSFFKKHGLAVPNTWDE
                     FVDLLARIKKIPGVRAPIASGNGVGWPLSDVTEHFIATYGGPAMTRDLISGKIAWTDP
                     KVRAVFENRLVPLLKAGYFSEPIEWTMAADLWWAGDYALYFMGSWITGMVKDPTDLGV
                     FTLPGARALVFATDYAFVPAYTKYPAEARKLLAHLATRGQTLQVAAGGHIATYTGAPL
                     EAYPPVDREVASLLKGLEALPDLDDTIGGEWQPAFWDQLKLLWVSPARVGEVLDNLQR
                     KAGK"
     gene            9812..10687
                     /locus_tag="PLM74_03265"
     CDS             9812..10687
                     /locus_tag="PLM74_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012003873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HON42137.1"
                     /translation="MHTLRGRALFFVPALALLAVFVVYPVVNTVWLSFFTSEGQFALL
                     GNYAEVLSQPEMFDPRGFTRGFPYGALVHNVMWVVIHLPLTAGLGLVLAVILREIKGG
                     AVVKAIIFLGMVTPMVVGGVILRFLFGGNVGILPSLMSLIGLPRRTLTAYPDTALFSL
                     IIGSLWLWTPFSMILYSAGLETIPDYVYEAAVIDGASATRAFFSITVPMLRPVTGVVI
                     TMTFLWVLKIFDIVYVATMGGPGGASNVLALQMYMYAFREFDANSAAVVATFLMLVAL
                     VVSIPTFRSVRGEQP"
     gene            10684..11535
                     /locus_tag="PLM74_03270"
     CDS             10684..11535
                     /locus_tag="PLM74_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HON42138.1"
                     /translation="MRSTSIFARRKSSTIALYVAAGLAALIWLIPFLGVFMASIRPLS
                     EISRGWWKFEQFNPTFRNYVGAWNHPAAPLGKGMLNSLRVAVPATILPLIIASIAAYG
                     FARFRFRLRDLLFMAVVALMTLPQQMIAVPIYRIMRGLGAVDSYTGLVLVHAAWGLPW
                     IMYFMRNFFTTVPIEVEEAAKVDGAFDLRVFLQIVMPMSLPAVASAAVLQFMWVWSDF
                     FLALILLYSPDKLLATQRVPLLRGVYHVDWGVLSAGSVIVMAVPIIVFALMQRYYIRG
                     MVGWTSK"
BASE COUNT         2391 a         3082 c         3706 g         2512 t
ORIGIN      
        1 tgtcggcttc gtcatcaatc ggtatcatga gaaggggttc gccagaggcg ccaagcgtga
       61 acagatcgct ctttgttcgg agttaggcat tgagctttcc gacttcgtgg gtgtgtgcct
      121 gcaagccatg cagcagcacg ccgcggagct gggcctgtga tgtagatacg caggcagcgg
      181 ccgggtttgc gcggggcctg gggcgtgggg gtgtgttggg tgtcgaatga gaagactcac
      241 gagaagcttg tagccactaa ccggaaggcc aggcacgagt atttcatcga ggagacatat
      301 gaggcaggca tcgccctgac cgggaccgag atccagagtg taaggcaggg ccgggtcagc
      361 ctgggcgacg cgtacgccga agtcagaggc agcgaggtgt ttctggtcaa ttcgcacata
      421 gcggagtatg actatggaaa caggttcaac cataacccca ggcgtccgcg gaaactgctg
      481 ctgcacaagt cggaaatccg gcgcctcgat tcgcgagttc gggagcgcgg gtataccata
      541 gtgccgctta ggatgtacct tcgggggggt ctggccaagc ttgagatcgg cctggcccgc
      601 ggcaagaaga cgtgggacaa gcgcgaagac atcgcaaagc gaaacgtgca gagagagata
      661 gagcgcacgg cgaaagcatc cgggcgcgag cgggagtagt catgacccaa gagtgacggg
      721 cgaaaaccgc ctgttgcatc gtttgttcgt cgcgtggtat gatactataa gcagtgatag
      781 ggggcgttac ggtttcgacg gggagtgctg tgaggggcgt ggcgagccga gatcccacct
      841 gctcgttaat cggtggaagc acgataagtg ccgataactc actagcatac gcagcctagt
      901 aatctaggtt gccgtccgac ccgggctcat tccgggagcc tggcaagggc gtcatttaag
      961 ccgggatgca cgtgcgccaa ttctcggagg cgcgcgccaa gataatcgag atagccctcg
     1021 tgcagcgcgc tcgtgcgcgg cacgttgggt gaaagcaagt acgcgggcta cgctcgtaga
     1081 agcgcgccaa gtggtcttct cggacccggg ttcgactccc ggcgcctcca ccaagtttgc
     1141 agagagtctt ttgtcggtgt gtggctccac tttgcctaga aaactgagta atcaagccat
     1201 tcctgcgtaa tagcggagcg gcttttacca tgttgtgaca gttctacacg gtatgcacgc
     1261 ttgccagtcc cggcaagcgt ttttgtctgc gcagactcgg aaacggtgcc aaacatgttt
     1321 ccgaaaccta ttgcagcccc gcatctcata tgatacaatg ctaacgaacg ttaggtagaa
     1381 aggtgttcgg tatggcagaa aaggatatta gagaaaggat taaagaagta gcagtagagc
     1441 atttcaacac cagtggttac catggaacaa cgatccgcaa catagctaag gacgtgaact
     1501 gctctttgcc aatgatctat tactacttca acagcaagaa ggagttgttt gatgaaatca
     1561 taaaaaagga gtatttcgaa gtcctgaaga ggcaagcagc actctcgaac acgagtaaca
     1621 ttgtagattt ctatacgaag tttgtgtaca acctaaactt cctaagcaac tatgataagc
     1681 aagtctaccg gcttgggatc aaggtctacc tgtccttcga tggcgatcag gaactgatgg
     1741 aaatcatgga cgagtgggaa aagaccatac ttccacgaca ctatcagata ctagagccac
     1801 atctgaaaga cgtagacaat ggcatcgcga ttgtacgaac gctagtacat ctattggaaa
     1861 cgttgataga aagcattgtc gtcaagaaca gattcctacc tgaagaagaa gtccgagagg
     1921 agatctcgat tgtactccgc ggttgtgaat gacctgtctt tcgtacttga cgggtgttag
     1981 tgtttgcatg acctaacgaa cgttatgaca aatagcatac tcaaaggaga gacagtaaaa
     2041 tggatattga gacgagactt gccctactgc aaaataccta tgcggcatcc atagcggaga
     2101 ccgtgaacac ctatgacaag ctaaaggtgc ttgacacaat cgtagagaga aggaaagaaa
     2161 ggcaagcaca gacagcacca tttttgaacc agcagcttgg tattgagact gttgaggatg
     2221 tgtttggaaa gctttcagag atctatggct gcgcaaactg gtctgtggaa acaacagaca
     2281 agggatatgt cgctacagca gtctcgtgca agttatgcgc gctgtccaag aagatgggag
     2341 gagcgaatcc ctgcaatgga tggtgcctag atcccatgtc agccatgatc actgccgcag
     2401 gcaagatcga tgctgaaagc ataattgtcg aaagcacttt aatggtcgga gactgctgca
     2461 aagtgctcat caatatctcc gcagaacagg atgatactgc tgagacttcg ctaacatatg
     2521 gtgatgatgg cccaaatacc agtttgccat cgagacggat cacttccggc tgttctatac
     2581 ttttgtgtta ggcacgatat gctcgacaca tgggaacgcc tacatgcgga acgtgaccta
     2641 gagaggaatc acgccgttgc cgcagaaact acatagagta catgcttgaa gcgaaggtgg
     2701 ttcgaactga gcgaaaagga cttgtctggc gacgatatgg cgcgacttgc acggttcgtg
     2761 gccgagcgcg gctgggaagt gtgccaggag cgggcgatcc agcacgggcg gcagatgaag
     2821 ataagcgacg ggcagaatca ggccacagtg gtcgtctact tcacagggaa gtcgctggtc
     2881 cagggtcgct catccagcct tacggacgag ctcgctgaat ggtgttcgac gagtctacgg
     2941 ggaagcgtct cccgcacgga agcacatgga ggtgggcgct tgccgaatcc agcgcatgca
     3001 tgcgggattc ccaggatagg gatagacgag tccggcaagg gcgactattt cggaccgatg
     3061 gtagctgccg gcgtatacgt tgaccgggga tcggagcttg tcctggtcaa gatgggggta
     3121 gtagacagca aacgtttgtc ggatgcgcgc attgcgaagc tcgcccaggc aatcaggagc
     3181 gaatgcccgc attctgtggt cgtcattggc ccggcccgct acaatcagct ctatgtgagt
     3241 atgcgcaatg taaatgtaat gctcgcatgg gcccattcga ggtgcctaga gaacgtgctg
     3301 acgcgggtgg agtgcggcct tgccgtctcc gatcaatttg gcgacgagtc ctaccttacg
     3361 caggccctta tggagagggg tcgaaagatc aagctggagc aacatccacg ggccgaggcg
     3421 gacgtggctg tggctgccgc ctctattctg gcgcgggacg agttcgtacg taggctttcg
     3481 gctctgtcgc agcaggcggg gatcgcgctc cccaagggcg cgtcagaccc tcggatagtg
     3541 gccgttgcat cggagatagc gtcgaagggc gggcgcgagg ctttagccga ctatgctaaa
     3601 ctgcatttca ggactaccga atcgctgggt atatagatat cgccttagcg cgtgggaggg
     3661 gaggcatgca gatggacggg gaaaacggct cgtcggagaa ccggaggagg cattgggcgg
     3721 gcatcgtgct catcattgtc ggctgctacc tgctggcatc gcagtttgtt tcgggcagga
     3781 tcatggggat gctggcggcg cctgggatcg gtgcgctcct cctgctatgg ggagcagtta
     3841 gcaggagtgt ggggctgatt gtcgtcggtg gcgttgtgtt cggcagcggc gcggggtctt
     3901 tcttggccag tgaggtgttc tggcgtctta agggccctgc caatgcggga gtgatcgtgc
     3961 tcagcatggc ggccggctgg tttatgataa tgttgttgtc tgaaaggttc gctggcaacc
     4021 cccagcgctg ggcgctggtt ccggccgtga ttatgaccgc tgtgggcgtt ccgttgatac
     4081 tgtcgagtgt ggcccccgtt gtgctagaat gggttgtcac ttggtggcct gccttgctca
     4141 tcatagccgg tttgctcctt ttcctccgca ggggaggcgg aggatgacag aggcgctgac
     4201 cgaggcggtg ccgtcggaac gcaacggaga tatcccctgg agataatgtg tgatgccgca
     4261 atcgattgga ggtacgctgc atacaatgag agccaggcag attcgcgaag gtatattctg
     4321 gatgggcgcg gttgactggg accgcaggct tttcgattcg ctgattcccc ttcctgacgg
     4381 aacaagctac aacgcgtatc ttgtcagggg cagcagcaaa accgctctgc ttgatacggt
     4441 cgacccgtct gtgtactatc aactcgacgc tcagctggcc tcggtggaac accttgacta
     4501 cgtagttgcg caccatgggg agcaggacca ctcaggctcg atacccgccg tcttagcgcg
     4561 ttaccccgag gcctcagttg tatgctctcc caagtgcaag cccatgctca tcgacctgct
     4621 tcacgtggcg gaggagcgca tcgtcgtggt tgacgatggc gacactatcg atctgggcgg
     4681 gcggactctc aagttcgtgc acgctccgtg ggtgcactgg ccggagacga tgctgaccta
     4741 tgtgcccgaa gacaagatcc tgtttacctg cgatttcctc ggcagtcacc tggcaactag
     4801 cgagctttac tcgaccgggc gacctcggga gcgcgaagcg gccaagcggt attttgccga
     4861 aatcatgatg cccttcagca acctcatcgt gaagaacctc gacaagatcg acggtctcga
     4921 cgtagatgtc gtcgcgccta gccatggccc agtagtgtct gatccgggtt acatcaccgg
     4981 ctgctacaga gaatggagcg ctggccccat gcgcaacaag gtatgcctgc cctatgtaag
     5041 catgcacggc agcacacgcg tgatggtcga cgcgctggtt tcggcgctgg taggccgcgg
     5101 cgtagcggtg gagcggtttg agcttacagg gtcggatctt gggaacgtgg cgatgtcatt
     5161 ggtggatgcc gccactgtgg tcatcggcac tccaacagtg ctcggcggtg cgcatccggc
     5221 tgcggctcac gttgctttgt tggccaatgc gctgcgccca cgcaccaagt tcgtatcagt
     5281 aatgtgctcc tatagctggg gcggtcgggc agtggaacag atctcggggt tgatcggcaa
     5341 cctccgggcc gaagtgctcg aacctgtggt gtgcaagggg ctcccgcgaa gccaggacct
     5401 gcaggcgata gagcgtttgg ccgaccagat cgcccagaaa catcgcgagc tggggttagc
     5461 ataagcggga aggcaagcca gcaaactggc tgggcaaacc gcgcagcccc agcccagtgc
     5521 gtatgcatta cccatacgta tgtctttcga cgaaaccgtg tttagcgggg agttagctta
     5581 cctgtaacaa tactgcacgg tttctgcggc gacctgtaca ggaagtgccc ggttttgacc
     5641 agcggcagga atgactcggg agtcattggc gtttcatgga gaatctgctg ctgcctaact
     5701 ccctctcggg catattcgag ggatagaggt acgttaccga attatgtgaa acgcctctac
     5761 atgcggattt gacggaggcg tgcctccggg aaaaggaggc gccgttgccg gcgcgaattc
     5821 acacaactcg tgcccgccaa accaggcagg atctgagcag gttgctcgaa aagcctgttc
     5881 aagagcgaac aggtgcctcg gagccccgtc agggcgtagg agaacgccgt accgcggaac
     5941 atccatggat atgggtaatg cgtagagagg tttgtaagag gaacaggagt tgtgcctcat
     6001 ggatgtaacc actcgtgaac ggtatctcgg aagcatgatc ggcctcgcag tgggtgacgc
     6061 cgtaggggcg caggtcgagt tcatgcgtcc tggggatttc gagcccgtcg tcgacatggt
     6121 gggcgggggc gtgttcgggt tgcctgcggg cgccttcacc gacgatacat ctatggccct
     6181 gtgtcttgcc cagtcgctgg tggagcgcgg tgggttcgat cccgttgatc agctcgagag
     6241 ataccttagg tggatgcggg aaggctacat gtccagcatc ggtcgggctt tcgatgtggg
     6301 gggcacatgc gccgatgcta tcgaaagatt cgaggccacg cgccggccct attgtggcag
     6361 cacgaatccc aatcgcgccg gcaacggctc gatcatgagg cttgcgccgg ttcccctgtt
     6421 cttcgcgcac gacccggaga aggcggtgga aatgtcggcg gcgagctcgc gaacgactca
     6481 cggagcccgg aacgccgttg acgggtgcag gtacttagga gcgcttatcg tgggcgctgt
     6541 cggcggtgca tcgaaagagc agcttctttc tccccggtac acgcccgcgc cgggtttgtg
     6601 ggagcgcgcg ccgctggccg acgccattcg agaagtcgcg gatggctcat tcgtaatgaa
     6661 gaaccctccc gagatacggg ggacaggcta cgtggtagat tcgctcgagg ccgcgttatg
     6721 ggcattccac cacggccaca gctttgagga cgggctcatg ctggcggtga accttggcga
     6781 cgatgccgat accacagccg ccgtgtacgg gcaattggca ggggcgttct acggggtttc
     6841 agcaattccc cagcgtttcc gtgaccgatt ggtgatgcgc gacctcatcg aggaattggc
     6901 ggagaagctc taccagtgcg cgtttgaggc gacgcatggt ggttcctgat tcggaacccc
     6961 cagcgtgcga ctgtcgcacg ctggggaaga tagtggggtg ccgggggctg tacatccgac
     7021 gaacagcccc cggcactttc gcatgttcat agagccgccc cctcggcccg cccatgtggc
     7081 tcccaggcgg ccaaacggta ttgagcggta ggctgtccac ctaaaggagg aattgggacc
     7141 tccatgtcga acaatacaac cggttgcaca agcgtgcaca tggtgccgat aaagcgccac
     7201 ttaaacccgg taaacatcgt aatgcaaccg tttggtgaat gcacgccgac ctgttctgct
     7261 gcggcgcagg acgacaatgc atcgatacgg ggatgcgtca atatgaagat gcgtcgatcc
     7321 gggaatgtgg cgcgacgccc tactgggatg caacagcagg cccacattgt gacatggcag
     7381 gagtgatcac gtgagtacta cgcttcaaga cattgcagac atgctcaaac tttccaagtc
     7441 cacagtgtcg agggcgcttt caggtgaccc tcgggtcgct gaagacacgc gggcgagagt
     7501 ggcgtcgctc gccaggcaca tggggtatac ccctaacccc actgcccgtg ccctcgccac
     7561 ccggcgaacg aatacgatcg ggctcgcggt gccgtgggcg ccgagatcgc tctctgaccc
     7621 tttctacctt gagtttctgg gcgctgcggg cgatgaggcc atgcgcagcg gatacagcct
     7681 gttcctgtcg acgcccgacg gggacggggc cggggctgtg cgcgctcacg cggaactggc
     7741 cgacccgcgc cggatagatg ggatgattct gacggagccc agggcgaacg acgagcgcat
     7801 cgcgctcttg cagagtgtcg ggctaccttt cgtcgttctc ggagtggtgc ccgacccgct
     7861 tgtctcgtgg atcagcggcg ataacacgac cggggcccgt gacgcagttg actatatgat
     7921 cagcctgggc cataccagga tagcgtgcat aacagggccg cccgaccaga ctgcttcgga
     7981 tgccaggttt gagggctacc ggctggctat gtccggcgcc ggcctgcccc tggatcgcaa
     8041 catcatagag gccggcgact tcacgcagtc aggggggtac tccgccatgc atgcgattat
     8101 cggctcgcgc ggtgttccgt ctgcggtgtt tgcatgtaac gatgtgatgg cgcttggggc
     8161 aatgcgcgca ctgcgcgagg cgggtcttgc ggttccttcc gacgtgtcgg tagtgggttt
     8221 cgacgggata tccatggcgg aatatgtgga cccgccgctc gcgacggtaa agcagcccat
     8281 ccaggagctc gggagaatgg cagtacagat tcttatcggg agaatgagcg gcgaaggcgg
     8341 tcctgtccgt tgcattctgc ctgtgcagtt ccaacctcgt gattcggtgg ggtcgccgcg
     8401 cctcggggca aacgcggctg atagggggtg atccggcaca cgatacacgt tccggctggt
     8461 atcagaagga tgtcagtaag cctgcagacc tatgcagacc actagaacgg agggattatg
     8521 atgcggcgaa aggtaacagt cttgctcctt attgtccttg ccgtcttcag tctgaccggg
     8581 ttggctgccg agaagaagct taccgtaata agcggatggt ctgggccgga gatggacgca
     8641 ttcatgccgg tgctccggga gttcgagaag gagactggga ttcgcgttga ctaccagatc
     8701 tatcgtgctg aggaccttgc cgttcttctt ccagctcaat tcgactcgaa gaccgctccg
     8761 gcagatgtga tcttcatgtg ggcatggttc atcaacaagc aagctcagag tggtcatgtc
     8821 ctggacatga ccggtgtgct caatgagtcg gatttcgtgc cgggtgctct tgacgccgtg
     8881 aagtcaggcg ccaagctgtg gggcgctccg tacaccggca agatcaagcc cggattctgg
     8941 taccggaagt ctttcttcaa aaagcatggt cttgctgttc ctaatacgtg ggatgagttc
     9001 gtggatctcc ttgcgcgcat taagaagata cccggcgtgc gtgcccccat cgccagcggc
     9061 aatggtgtgg gctggccgct gtcggacgtg acggaacatt tcatcgctac gtacggcgga
     9121 cccgccatga cgcgtgatct gatttccggg aagattgcct ggaccgatcc gaaggtccgc
     9181 gccgtgttcg agaaccgctt ggtgccgctg ctcaaggcgg gctacttcag cgaacccatc
     9241 gagtggacta tggcggcgga tctgtggtgg gcaggggact atgctctgta cttcatggga
     9301 agctggatca ctggaatggt aaaggacccg accgacctgg gagtgtttac cctccccggg
     9361 gctcgggcac tggtgttcgc caccgactac gcctttgttc cagcgtatac caagtatccc
     9421 gctgaggcca gaaagctgct cgcccatctc gctacccgtg gccagacgct gcaggtagct
     9481 gcgggcggcc acattgctac atacacgggc gcgcctcttg aggcatatcc tccggtggac
     9541 cgcgaggtcg catctctgct caaggggctt gaggccctgc cggacctgga cgatacgatt
     9601 ggcggagaat ggcaaccggc cttctgggat cagctcaagc tgctctgggt gagcccggca
     9661 agggtagggg aagtcctgga taacctccag cgcaaggcgg gcaagtaggg caagcagatc
     9721 gtaagcagat ggacaaggca ctccggccgg ccgccgcgtc cgagcggcga ccggtcgggc
     9781 ctttacggag cacctcgaag gaggcgggaa catgcacaca ttgcgtggca gggccctttt
     9841 cttcgttccg gccctggcgc ttctggcagt gtttgtggtt taccctgtcg tcaatacggt
     9901 atggctgagc ttcttcacaa gcgaaggcca gtttgcgctg cttgggaact acgcggaggt
     9961 gctttcgcag cccgagatgt tcgatccgcg cggtttcacg aggggatttc cctatggtgc
    10021 gctggtccac aacgtgatgt gggtcgtcat acatctgccg ctgaccgcgg gcctcggtct
    10081 ggtgctggcg gtgatcctcc gtgagatcaa gggaggggct gtagttaagg ccataatatt
    10141 ccttgggatg gtcacgccca tggttgtggg tggagtcata ctgaggtttc tgttcggcgg
    10201 gaatgtgggc atcttgccat ctctgatgtc gctcatcggg ttgcccaggc gcacccttac
    10261 cgcctatccc gacacggcgc tgttttcgtt aatcatagga tcgctctggc tatggacgcc
    10321 attcagcatg atcctgtatt cagcaggcct tgagaccata ccggactatg tgtacgaggc
    10381 cgctgtgatc gatggcgcgt cggcgactcg ggcgttcttc tccatcacgg tgccgatgct
    10441 acggccggtt accggcgttg tgatcaccat gacttttctc tgggttctca agatattcga
    10501 catcgtgtac gtggctacta tgggcggccc cgggggcgcg tcaaacgtgc ttgcgctgca
    10561 gatgtacatg tacgcgtttc gtgagttcga cgcaaactca gcggctgtgg tagccacatt
    10621 cctgatgttg gtggccttgg tcgtgtcgat accaacattc aggtccgtaa ggggtgagca
    10681 gccgtgagaa gcacatccat cttcgcgcgc cggaagtcat ctaccatagc cttgtacgtt
    10741 gctgcggggc ttgcggctct gatctggctc attcctttcc tcggggtatt catggcatcg
    10801 atacggccac tgtccgagat cagtcgagga tggtggaaat tcgagcagtt caaccctacc
    10861 tttcgaaact acgtgggcgc atggaaccac cctgcggcgc cgctgggaaa gggcatgctt
    10921 aattccctgc gtgtggctgt tcccgccacc attctgcctc tcattattgc gtcgatcgcg
    10981 gcgtatggtt tcgcgcggtt ccgctttcgc ttaagagact tgctcttcat ggccgttgtg
    11041 gcgcttatga cgctgccgca gcagatgatc gcggttccaa tataccgcat catgcggggc
    11101 ctcggcgcag tggactccta caccgggcta gtactggtac atgcggcctg ggggttgccg
    11161 tggatcatgt atttcatgcg caacttcttc accacagtgc ctatcgaggt ggaggaggcg
    11221 gcgaaggtgg acggtgcgtt cgacctccgg gtcttcttgc agattgtcat gcctatgtcg
    11281 ctgcctgctg tagcttcagc agctgttctg cagttcatgt gggtctggag cgacttcttc
    11341 ttggccctaa tattgctgta ctcacccgac aaactcctgg ccactcagcg cgtgcccctg
    11401 ctccgcggcg tctaccacgt tgactggggg gttctctctg ccggcagcgt catagtcatg
    11461 gcagtgccca tcatcgtgtt cgcactcatg caacggtact acataagggg catggtcggc
    11521 tggacttcga agtagctaat aaggtctttc ggagggatgg atgatggcct tcacacgagt
    11581 ggatatcgca cagaagcggc tggacgatca cgccccgtat gccggcgaag ccgcggtggc
    11641 ggatgcgagg catttggcgc ggaggctcga gggcgcgcgg gtgctgcacg t
//
LOCUS       DAOTAC010000046        11518 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_4777_length_11518_cov_3.890430, whole genome shotgun sequence.
ACCESSION   DAOTAC010000046 DAOTAC010000000
VERSION     DAOTAC010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 11518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11518
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4777_length_11518_cov_3.890430"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="PLM74_03275"
     CDS             <1..274
                     /locus_tag="PLM74_03275"
                     /inference="COORDINATES: protein motif:HMM:NF015402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HON42139.1"
                     /translation="VIVSGKHTGFPTRMDDVRAVKRVLPEVPVLIGSGLRQSSAAEFL
                     EVADGAIVGSSLKQDGDAANPVDCERVRSLMEVVRRVRETREAERA"
     gene            314..1249
                     /locus_tag="PLM74_03280"
     CDS             314..1249
                     /locus_tag="PLM74_03280"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HON42140.1"
                     /translation="MCRHEYDLVVIGDSNTDIYARVSSYPAAGGCTFGERLESLPGGT
                     AVNTAVAAARLGLRVAIATVVGHDAEGQAILDLLQSEGVDARFAARVPQPTGCVFVAL
                     GPGEEHTFFSLRVECADWQLRREHIPFEAIRESAALYVSGVSIAEAGIVKGSADSVLA
                     ALRIARDAGATTFLDPNLRLESPDVPIDIAQAMETCIRTVDYYMPNESEMEALALRCG
                     QLHGRVATVVKQGSRGCTVISGSGRSQIAAKPVRAVDTTGAGDSFNAGFIAGILGGRP
                     ADGAGRLGAIVAGITVSRVGTISAFPTRSEVEQEG"
     gene            1252..2277
                     /locus_tag="PLM74_03285"
     CDS             1252..2277
                     /locus_tag="PLM74_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HON42141.1"
                     /translation="MSVKFRKVLLVLAILSLAVLQVAPGSAKTVLRVAVVAGDRIGDR
                     GFTDSAREGLVRAAKDFDIQYKIFECKADASLYYDRTLAAAENFDLVFMDPGYFFDKE
                     LSEIAPKFPKVTFVYIDGTSDIPGVVSIDFKEHEGSFLAGALAGLMASNPKLKLGKPG
                     KVVGFVGGADWPVIRNFLAGYEQGVKYVDPSIKVESVFAGTHYDPAKGKEATLQAHKR
                     GANIVFQAAGPSGLGVLEAARDAGFYAIGVDIDQCDSQPGFIMASMLKRVDNAVYDTI
                     KLAVEGKLAKGTVFPYGVRERGVGLCECKHMEDAVPKDVFEKLRDIEAKIARGEIVVN
                     EYTPTSK"
     gene            2377..3921
                     /locus_tag="PLM74_03290"
     CDS             2377..3921
                     /locus_tag="PLM74_03290"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON42142.1"
                     /translation="MEQAAVRLNNITKRFGKLAANDDVTIEVRRGEIHAIVGENGAGK
                     STLMNVVYGLVWPDEGSISINGVERSIRCPSDAINLGIGMVHQHMMQVGSYSVLENII
                     LGREPRTKWGSTDRKCARAAIAQLSSGFGLNVDMDQRVCDLPVGLRQRVEIVKLLYRG
                     ADIIVLDEPTAVLSPQGTEELLAVMRNLRSQGKTILFISHKLPEVLSVADRISVMRKG
                     KLVATVESGAATEEKLVELMIGHWPARTSGSARSAAGQGVLGVEGLCVSEPGSASGLR
                     NVHLQVRAGEIVAIAGVEGNGQRELAGAIIGIARPTKGRMFLDGQEITHLPVRARRER
                     GMAFVPEDRVDLGLVLNATVTENAILGPAYRTSPVSKGWIMNWSAADSAARRIIDRYR
                     VKMSGPARTSVMGSLSGGNMQKLLVGRELAGDVKCMVICQPTRGLDIGAKAGIYDALF
                     AMRDAGCAILAVSTDLDEIFEIADRVVVMYKGEIAADLARADSSPEEVGLYMTGVRSD
                     RVGPGF"
     gene            3905..4957
                     /locus_tag="PLM74_03295"
     CDS             3905..4957
                     /locus_tag="PLM74_03295"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HON42143.1"
                     /translation="MGRGSEAWNDMATFIVAFAVALLMGAVVIAATGSSPWDAYRAAL
                     RAAAGSGYRFLNTLCNTTVLIMTGMACSVAFNSGIQNIGAEGQLYLGAFAAAMAGLIR
                     GIPAFVHVPLTLAVAACAGAVWALMPGILRASRGTNEVVVTLMMNYIAVLLCSYFVNY
                     PFRNAAATWPETAMIEASARLPYLHPLSRFNGSFFVALACVVMIVFINRRTRTGYEWR
                     MVGLNERACTVAGIVPGKVMLQAMIVSGAIAGLAGGAEVIGVRHRFMDAFSPGYGMTG
                     VLIALVARNNPVSVALVAFIFSLVKTGAGGMELVADVPAELSEILLTLIVFMLAAQRS
                     LRGTLRAARTQQHAGR"
     gene            4962..5888
                     /locus_tag="PLM74_03300"
     CDS             4962..5888
                     /locus_tag="PLM74_03300"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HON42144.1"
                     /translation="MGWAVDVADLLGATFRLATPVVLAAIGGLVSFHAGIMNVAMEGF
                     MLISAFTAVLVSYYAGAWAGVLVAVAVCLAFSALYALFVVDLQSDGFAIGFALNILAT
                     GLTIYLVRILGTDIFNSPEIQPLPHLGIRALAASPILNAILNGHSVTVYVTPVLVWAM
                     SAVLYRTPFGAHLRAAGEAPAALAASGVSPRRVRYAASLICGAFCGMAGAHLSLGYLT
                     QFIRDMTAGRGFMALAAILVGRGRPAATALVGLLFAASEAISMKIQAYAAPQFALMLP
                     YVVTIIAVTFMSGKPKKNVSSAPEETAETATE"
     gene            5896..5973
                     /locus_tag="PLM74_03305"
                     /pseudo
     CDS             5896..5973
                     /locus_tag="PLM74_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016562212.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-specific adenosine deaminase"
     gene            complement(6075..6350)
                     /locus_tag="PLM74_03310"
     CDS             complement(6075..6350)
                     /locus_tag="PLM74_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42145.1"
                     /translation="MHHDEIVGVANTIPLWWDRPFEELPEEGTRPNTLGGLQAAIIPA
                     CRGRELSSSILLEMISLARRDGHRALILPVRPSRKRASTLFRALSCL"
     gene            complement(6441..6929)
                     /gene="nrdG"
                     /locus_tag="PLM74_03315"
     CDS             complement(6441..6929)
                     /gene="nrdG"
                     /locus_tag="PLM74_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HON42146.1"
                     /translation="MTDAMPMTVKLAGIVDESVVDGPGVRLVVFAQGCPHRCRGCHNP
                     HTWDFDGGTEVSIEQILLRVEANPILSGITLSGGEPFAQAPGFARLARKARRRGLSVM
                     TYTGYTREHLVDSLAPGVRDLLLATDILIDGPFISALADPGLPYRGSRNQRIIQVRDR
                     VP"
     gene            complement(6926..8878)
                     /gene="nrdD"
                     /locus_tag="PLM74_03320"
     CDS             complement(6926..8878)
                     /gene="nrdD"
                     /locus_tag="PLM74_03320"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HON42147.1"
                     /translation="MAVARRYYPIHSTRPARSIRPTCPASHAYAAHSPARAHVLGKSS
                     PPNLMDSIAEILKETSRENANIGNSPSAKVLQIASEASKEYYLTQVIPADISRAHQDG
                     DIHIHDLDFYGKTLTCVQIPLGRLLADGFNTGHGYVRPPKRPASAAALAAIIIQSCQN
                     DMHGGQSFPFFDADMAPYMEGRSEDEVYQAMEALVFNLNSMHSRAGAQVPFSSLNLGC
                     DTSQEGRLTSKCLLEAYEAGLGRGETPVFPNIIFRVKAGVNASPGDPNYDLFLLACRV
                     AARRMNPTFSFMDSSFNAPYGSEVSYMGCRTRVIANRHGAAVSAGRGNLSFTSVNLPR
                     LALRAGGNEDRFFILLGRCMDLAARQLLHRCNVQSRLRVRDMPFLMGQHIYMGSEELC
                     ADDDIAPAIKHGTLSIGFIGLAETLTVLRGAGRGKSAEVSPGSGVGFGIAASDEAGVL
                     GYEIVRFMRNRVDEYADAHDLNFTLLATPAEGLSGRFVAIDRERFGTIRGVTDRAYYT
                     NSFHVPVGAHMSIYDKLAREGKYHSLTNAGHISYVELASPPLHNVEAVESIVRAMVRA
                     DLGYGGINFPIDECLACGYSGVIESVCPACQSGSIRRVRRITGYLSTVDRFNDAKRAE
                     LADRAAHMRDAEGARTGAHKRGAERR"
     regulatory      complement(8906..9130)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(9281..10291)
                     /locus_tag="PLM74_03325"
     CDS             complement(9281..10291)
                     /locus_tag="PLM74_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42148.1"
                     /translation="MITRRNARLVLHVLCLALTVMPTIRTISATQAPGPMEVSSTADI
                     AEVAPAGSIICCSTKSGETSMVDISTGTVQILPFSGSSIEISPDRKTLAYVDRREQLH
                     VYHLDSGIDVRLGDPEESEKYGSPTFVSNDTIAYVKRNRSYRTAVCLTHIDRLDERPW
                     VGELPEGVSTSSSTLKWIPGTGEELYSFVLGNVYSIYVLAPHGSTTLVSADPKSGASL
                     RYPAVSPDGATIAYVHKSSISSIWTINIDGRNKRQLTYSDGSWPTWSPDGLHIAFLIH
                     AAGERGLLKQDIYDPSGTDRSVGSFLFGIGIIRRDGTPVRSVCGVDGKPVLTPGDYIA
                     WR"
     gene            complement(10354..10674)
                     /locus_tag="PLM74_03330"
     CDS             complement(10354..10674)
                     /locus_tag="PLM74_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014795039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HON42149.1"
                     /translation="MADRVTQEALMSIGRRYAEVLRRELTLRSLYVFGSYATGKYGDD
                     SDIDIAVVSDDLTGDMIEDTFALMRLRREVDTRIEPHPFLTSDFTENNPIAREIMETG
                     IRIL"
     gene            complement(10644..11045)
                     /locus_tag="PLM74_03335"
     CDS             complement(10644..11045)
                     /locus_tag="PLM74_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015759407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HON42150.1"
                     /translation="MQKDELVAYWLDTANRDYQTVKHLYDSGDYHWSLLMGHLVIEKL
                     LKAVLIACQPEGTSVPRSHDLLLLAEKSGIGMTQRQKDLLDLTTTFNVAARYPDYQQS
                     FYRKCTREYTAERMAEIEELWAWLTELLKKR"
BASE COUNT         2257 a         3198 c         3662 g         2401 t
ORIGIN      
        1 agtgatagtc agcggcaagc acacgggctt tccaacacgc atggatgatg tgcgtgccgt
       61 gaagcgagta ttaccggaag tgcccgtcct catcggcagt ggcctgaggc agtcaagtgc
      121 ggcagagttt ctggaagtgg ccgatggggc catagttggg tcatcgctga aacaggacgg
      181 cgatgctgca aatccggtgg actgcgaacg agttcgttcc ctaatggaag tcgtgcgtag
      241 agtacgggaa acacgggaag cagagcgtgc ataatccgta gtcgctacgg gacgagcgcg
      301 gggggccatt tgcatgtgtc gacacgaata tgacctggta gttatcggcg actccaacac
      361 cgacatatat gctcgcgtca gcagttatcc tgccgcaggg ggctgtacgt tcggcgaacg
      421 gctggagtcg ctaccggggg gaacggctgt caacacggca gtagcagcgg cccgcctggg
      481 tctgagggta gccatagcta cggttgtcgg gcatgacgcc gaggggcagg cgatattgga
      541 cctgctccag agcgaaggcg tagatgccag attcgccgcc cgagtgccgc aacccaccgg
      601 atgcgtgttc gtggcgctgg gcccaggcga ggagcacacg ttcttctcgc tcagggtcga
      661 gtgcgctgac tggcaacttc ggcgtgagca cattccgttc gaggctatcc gggaatcggc
      721 tgcactgtac gtgtctggag tatcgatagc agaggccgga atagtgaagg ggtctgctga
      781 ctctgtgctg gctgcgctgc gcattgcgcg cgacgcgggc gcgacgacat tcctcgatcc
      841 caacttgagg cttgaaagcc cggatgtgcc catagacatc gcccaggcca tggagacatg
      901 cattcgcacg gttgactact acatgcccaa cgaatcggag atggaggcgc tcgctttacg
      961 ctgcggccag ctgcatggcc gggtggctac cgtcgtcaag caggggagcc gggggtgtac
     1021 ggtcatatct ggctccgggc ggtcgcagat cgcggccaag cccgtccggg cggtcgatac
     1081 aaccggcgcc ggcgactcat tcaacgccgg cttcatagct gggatacttg ggggcaggcc
     1141 tgcagacgga gccggcagac tgggtgcgat cgtggcaggt attacagtgt cgcgggtagg
     1201 gacgatatct gctttcccga cgaggtcaga agtcgaacag gagggatgat catgtctgtc
     1261 aagttcagaa aggttcttct tgtcctagcg atcctaagcc tcgcggttct tcaggtagcg
     1321 ccgggctcgg caaagaccgt tctcagggta gctgtggtcg ccggagaccg aataggcgac
     1381 cgaggtttca ccgattcagc gcgcgagggc ctggtcagag cggcaaaaga cttcgacatc
     1441 cagtacaaga tctttgaatg caaagccgat gcgtctctct actatgacag aacgcttgcc
     1501 gcggcagaga acttcgatct ggttttcatg gatcccggct acttcttcga caaggaactc
     1561 tctgagatcg cccccaagtt ccccaaggtc acgtttgtct acatagacgg tacgtccgac
     1621 ataccgggag tagtatcgat cgacttcaag gagcacgaag gttcgtttct ggcaggtgcc
     1681 ctagccggac ttatggcgtc taaccccaag ctcaagctgg gtaagcccgg taaggtggtc
     1741 ggatttgtcg gaggcgccga ctggcccgtc attcgcaatt tcctcgcagg atacgaacag
     1801 ggggtcaagt acgtcgaccc atcgatcaag gttgaatcgg tgtttgccgg cactcactac
     1861 gatccggcca aggggaaaga ggcgacgctt caggcgcaca agcgcggcgc gaatatcgtg
     1921 ttccaggcag ctggcccgtc gggcctgggt gttcttgagg ccgcgcgcga cgcggggttc
     1981 tacgcgattg gagtagacat cgatcagtgc gacagccagc caggtttcat catggcgagc
     2041 atgctcaagc gagtggataa cgcagtatat gacacgatca agctcgcggt agagggtaag
     2101 ctcgcgaagg gcacggtctt cccgtacggg gttcgggagc gcggcgtggg gctgtgcgaa
     2161 tgtaagcata tggaggacgc tgtgccgaag gatgtctttg agaagcttcg cgacattgaa
     2221 gccaagatcg cgcggggcga gattgttgtg aacgagtaca cgcccacgtc taagtgacta
     2281 tcggaccgga ggagagaaca acctgagcag ataacctgag gagatactcc atgagtggcg
     2341 aggcgtacgc ctcgccactt tcaggggggc cacgcgatgg aacaggcagc agtaaggctc
     2401 aacaacatta cgaaacggtt cggcaagctc gccgcgaatg acgatgtcac tatcgaggtg
     2461 cgcagaggag agatacatgc catcgtgggc gaaaacggag ccggcaagtc tacacttatg
     2521 aacgtggtat atggtctagt ttggccggat gaagggagca tatccatcaa tggagtcgag
     2581 cggagcataa ggtgcccgtc tgacgccatt aacttgggaa tcggcatggt ccatcagcac
     2641 atgatgcagg tgggctcgta ttctgtactg gaaaacatca tcctgggtag ggaacctcgg
     2701 accaagtggg ggtccaccga ccgaaagtgc gcccgtgcag ccatcgccca gttgtcgtcg
     2761 ggattcgggc taaatgtgga catggaccaa cgcgtgtgtg accttccggt tgggctaagg
     2821 cagagagtgg agatagtcaa actactgtac aggggtgccg acatcatagt tctcgacgaa
     2881 cctacagcag tactaagccc tcaggggacg gaagagcttc ttgcggtgat gcgtaacctg
     2941 aggtcgcagg gcaagacgat cctcttcatc tcgcacaaac tgcctgaggt gctctcagtt
     3001 gcggatcgca taagcgtgat gcgaaaaggc aaactcgtgg ccacagtcga gtctggcgca
     3061 gcgactgagg agaagctggt ggaattgatg ataggccact ggcccgccag aacctcaggc
     3121 agcgcacggt cggccgcagg ccagggcgtg ctgggggttg agggattgtg cgttagcgag
     3181 cccggcagtg cctcaggcct gcgcaacgtc caccttcagg ttagggcggg cgagatcgtg
     3241 gccatcgccg gagttgaagg caatgggcag cgggagcttg cgggcgccat cattgggata
     3301 gcaaggccga cgaaaggcag gatgtttttg gacggacagg agatcacaca cttgcccgtg
     3361 cgcgcgcggc gtgagcgggg gatggctttt gtgcccgagg accgtgtcga tcttggcctt
     3421 gttctaaatg ccacggtgac cgaaaacgcc atactcgggc cggcctatcg cacgagcccc
     3481 gtgagcaaag gttggatcat gaactggagc gcggccgaca gtgctgcccg acggattatc
     3541 gatagatatc gggtaaagat gtcgggcccg gcgagaacaa gcgtcatggg gtcgctttcc
     3601 ggaggcaata tgcagaaact gctggtggga agggagctcg ccggcgatgt gaagtgcatg
     3661 gtgatatgtc agcccactcg gggactggac attggtgcga aggctggcat atacgatgcg
     3721 ttgttcgcca tgcgcgacgc agggtgtgca atcctcgccg tcagcactga cctggacgag
     3781 atcttcgaga tcgcagatcg agtagtggtc atgtataagg gtgaaatagc cgccgatctt
     3841 gcgcgcgcgg atagctcgcc tgaggaagtc gggctataca tgacgggagt caggagtgat
     3901 cgtgttgggc cggggttctg aggcgtggaa tgacatggcc accttcattg tggccttcgc
     3961 tgtggccctg ctcatgggcg ccgtagtgat agcggccacg gggtctagcc cgtgggatgc
     4021 gtacagagcg gctctgcgcg ctgccgccgg aagcggatac cggtttctca atactctatg
     4081 caatactact gtgctaataa tgacaggcat ggcgtgttcg gtggcgttca actcggggat
     4141 ccagaatatc ggcgcggagg gccagctgta cctaggggct ttcgctgctg caatggccgg
     4201 actgataagg gggattccag cgtttgtgca cgtgccgctg acgctggccg tggccgcctg
     4261 cgcgggggcc gtatgggcct tgatgcccgg gatcctcagg gcatcgaggg ggaccaacga
     4321 agtagtagtg accctgatga tgaactacat tgctgtgctg ctatgctcat atttcgtaaa
     4381 ctacccgttc cgaaatgcgg cagctacatg gcctgagact gctatgatcg aggcttcggc
     4441 ccgcctgccg tatctccacc cgctgagtcg gttcaacggc tcatttttcg tagcccttgc
     4501 gtgcgtggtc atgatcgtct tcatcaatcg caggaccaga acagggtatg aatggcggat
     4561 ggtcggcctc aacgagcgcg catgcactgt agcaggcatt gtgccgggca aggtcatgct
     4621 tcaggcgatg atcgtgtcgg gagcgatcgc gggattggca ggaggggccg aagtcatagg
     4681 cgtacgtcac aggttcatgg acgccttttc gcccggatac gggatgacag gggtgttgat
     4741 agcgctggtg gccaggaaca acccggtctc ggtggccctt gtcgcgttca tcttctcgct
     4801 tgtgaagacg ggggccggcg gaatggagct cgtggcggac gtgcccgctg aactgagcga
     4861 gattctgctc acactgatcg tcttcatgct ggccgcgcag aggagtctgc gcggtacgtt
     4921 gcgcgctgct aggacgcagc agcatgccgg gagatgagga aatgggttgg gcagttgatg
     4981 tagccgacct gctcggcgcc acgtttcgat tggccacgcc tgtcgtgctc gcggccatcg
     5041 gcggattggt gtcgttccac gcaggcatca tgaacgtcgc catggaagga ttcatgctaa
     5101 tatcggcgtt tacggccgtt ctggtaagct actacgctgg agcgtgggcg ggcgtactcg
     5161 tggcggtagc cgtatgcctg gcctttagcg ccctctacgc attgttcgtc gtagatctgc
     5221 aaagcgacgg attcgcgata ggattcgcgc ttaacatact tgctaccgga ctgactatct
     5281 acctagtcag gatactcgga accgacatat tcaactcacc cgagattcag ccgctcccac
     5341 accttgggat ccgtgctctc gccgcatcgc ctatcctgaa tgccatccta aatgggcatt
     5401 cagtgactgt gtacgtcact ccggttctgg tatgggcgat gtctgccgta ctctaccgga
     5461 ctccgttcgg agcgcatctg cgcgccgcgg gagaagcgcc ggcggcgctc gccgcatccg
     5521 gtgtgagccc gcgcagggtg agatacgctg catccctaat atgcggcgct ttctgcggta
     5581 tggcaggcgc gcatctttcg ctggggtacc tgacgcagtt catcagggac atgactgcag
     5641 ggcgtgggtt catggccctt gccgccatct tggtcggcag aggacgccct gcggcaacag
     5701 cgcttgtggg cctgctgttc gcggcgtcgg aggcgatatc tatgaagatt caggcgtacg
     5761 ctgctccgca gttcgctctg atgctgccct acgtagtcac gatcatcgcc gtcacgttca
     5821 tgtcgggcaa gccgaagaag aacgtctctt cggcgccgga ggagacagcg gagacggcaa
     5881 ccgagtgagg gtacgcggga ggcgcttgcg gaggcggaga aggcgctggc cagaggggat
     5941 aggcctatcg gcgccgtgat cgtccacaac ggctaggtag tggccagggg ctcgaatacc
     6001 ttatccactg actgtgtgta ctgtccacac gtttggctcg acatcctctc cgatatcgcg
     6061 cgaacggtca atatctacag gcacgagagc gccctgaaca atgtacttgc ccgtttccgg
     6121 ctgggcctca caggcagtat caacgcgcgg tgcccgtctc gtctggccag ggagatcatc
     6181 tccagtaata tggacgagct cagctcccgg cctctgcacg ctgggataat cgcagcctgc
     6241 aggccgccga gggtgttggg ccgagtgcct tcctcgggaa gctcctcgaa aggcctatcc
     6301 caccacaacg ggatagtgtt ggcgacgccc acaatctcgt cgtggtgcat gtctttcggt
     6361 gctcctgcag cagatccgga cgttcggcga tagagaatac ctgatactcc attggatcac
     6421 cccatctctc ggcgctaaga tcatggtaca cggtcgcgca cctgaattat cctctggttc
     6481 cgcgagcccc ggtaaggcag gcccggatcc gcgagcgccg agatgaaggg gccgtcgatc
     6541 aggatatcgg tggccagcag caggtcgcgt acgccggggg cgagggagtc gacgagatgc
     6601 tcccttgtat accctgtgta ggtcatgact gatagtcctc tgcggcgggc tttgcgggcc
     6661 aggcgggcga atccgggcgc ctgggcgaag ggctcgccgc cgctcagggt gatgccgctc
     6721 agtatagggt tcgcctctac gcgaagcagg atctgctcga tactgacctc ggtacctcca
     6781 tcgaagtccc atgtgtgcgg attgtggcac ccccggcacc gatgcgggca cccctgagca
     6841 aacacaacca gtctcacacc gggcccatcc actacgctct cgtccacgat gccagctagc
     6901 tttacggtca tgggcatggc gtctgtcatc gtctttctgc tccccgcttg tgcgccccag
     6961 tcctggcgcc ctcggcatcc ctcatgtgcg ccgccctgtc ggcgagttca gcgcgcttgg
     7021 cgtcattgaa cctgtctacg gtggacagat acccggtgat tcgcctcacg cgtctgatcg
     7081 accccgactg gcacgccggg cagactgact cgatgacccc ggagtagccg catgcgaggc
     7141 actcgtctat cgggaagttg atgccgccgt atcccaggtc cgcgcgcacc atggcacgga
     7201 caatgctctc gacggcttcc acattgtgca gcggcgggga ggcgagctcc acgtaggaga
     7261 tgtggccagc attggtcagg ctgtgatact tgccctcgcg tgcgagttta tcgtagatcg
     7321 acatgtgggc acccaccggc acatggaaac tgttcgtgta gtaggcgcgg tcagtgacac
     7381 cccggatcgt gccgaacctc tctcgatcga tggcgacgaa ccttccggat aggccttcag
     7441 ccggcgtcgc cagcagtgtg aagttgaggt cgtgcgcgtc agcgtattcg tcgacgcggt
     7501 tgcgcatgaa cctgacgatc tcgtagccca acacgcctgc ttcgtcactg gcagcgatgc
     7561 cgaacccgac gccgctcccg gggctgacct cggcgctctt gccgcgccca gctccgcgca
     7621 ggacggtcag ggtttcggcc agtccgatga atccgatgga cagagtgccg tgcttgatcg
     7681 ccggcgcgat gtcatcgtcg gcgcacagtt cttccgagcc catgtagatg tgctgaccca
     7741 tgaggaaggg catatcacgc actctgagac gggattgcac gttgcatctg tgtagtagct
     7801 gcctggccgc caggtccata cagcggccga gcaggatgaa aaagcggtct tcgttgcctc
     7861 cggctcgcag ggcgagtcgc ggcaagttca ctgaagtgaa ggaaaggttg cctcgtccgg
     7921 cgctcacggc ggcgccgtgc ctgttcgcga ttactcgcgt tctgcatccc atgtaggaga
     7981 cctccgaacc gtagggcgcg ttgaatgagg aatccatgaa gctgaacgtc gggttcatgc
     8041 gccttgccgc cacgcggcac gcgagcagga agagatcgta gttcggatcg cccgggctcg
     8101 cgttgacgcc tgccttgact ctgaagatga tgttgggaaa gacgggcgtc tcgcctctgc
     8161 cgagtcctgc ttcgtaggcc tctagcagac acttggatgt gagcctgcct tcttgggaag
     8221 tgtcgcagcc caggttgagg ctggagaatg gcacctgagc gcctgccctg ctgtgcatgg
     8281 agttgaggtt gaacaccagc gcctccatgg cctggtagac ctcgtcttct gagcgtcctt
     8341 ccatgtacgg ggccatgtcg gcgtcgaaaa atggaaacga ctgtcctcca tgcatatcgt
     8401 tctgacagct ctgtatgatg attgcagcca gagcagcggc ggacgctgga cgtttgggcg
     8461 ggcgcacata gccgtgaccg gtgttgaaac catccgcaag cagcctgccc aggggtatct
     8521 gcacgcaagt cagggtcttg ccgtagaaat cgaggtcgtg tatgtggatg tctccgtcct
     8581 ggtgcgcccg ggagatgtcg gcggggatca cctgagtgag gtagtactcc ttgcttgctt
     8641 cagatgcgat ctgaagcact ttggcagacg gggaattgcc tatgttggcg ttttccctgc
     8701 tggtctcctt gaggatttct gcgatggagt ccatcagatt gggcggggaa gacttgccga
     8761 gaacgtgcgc acgcgcgggc gaatgcgccg cgtaggcatg ggaagctggg caagttgggc
     8821 gaatcgagcg agctgggcga gttgagtgaa tggggtagta acgacgtgca acagccatgg
     8881 tcaggcctcc ctttctccac gaaggtggct tgcatccccc gaaggcaggt ctcctggctg
     8941 ccggatcatc gcatgtgcgc gtcttccccc tgaataagca ggagtgactt gcggcgtgcg
     9001 tccatacttc ccggttacag tggcgggacc gcgggcttgg caggcgacac aacacccgat
     9061 ggtgcgtact cgcccgctgc ctcttcccta ttctccctca tacgagggca cctggcaggg
     9121 gtcgcctatt tggtcttgac catctcgaat gcggctagag tataggtcgt cgcgccgcga
     9181 tcgggatgtg tagttacaat ataacacaac gagcggcagt gagtaagctc acctgggtgc
     9241 gcgggtgggt gcatcgcacc ttcgacgcgc acaagagtgc ctatctccac gcgatgtaat
     9301 cgcccggcgt caacacgggt ttgccatcga ctccgcacac tgacctgact ggagttccgt
     9361 cgcggcgtat gatgcctatt ccgaagagga aggatccgac gcttctgtcg gtcccggacg
     9421 ggtcgtagat atcctgcttt agcaggccgc gttcgcccgc ggcgtggatg aggaaggcga
     9481 tatgcagtcc atccggcgac catgtgggcc agctgccgtc ggagtaggtg agttgccgct
     9541 tgtttcttcc atcgatgttg atggtccaga tagaactgat tgaagacttg tgcacgtagg
     9601 cgatggtggc gccgtccggg cttacggccg gatagcgcag cgaggcgccg cttttcgggt
     9661 ctgcggatac gagcgtcgtg ctgccgtggg gggcgaggac gtagatgcta tacacgttgc
     9721 ccagaacgaa cgaatacagc tcttcccctg tgcctgggat ccacttcagc gttgacgacg
     9781 atgtagaaac accttcgggc aattcgccta cccaggggcg ctcatcgagg cggtcgatgt
     9841 gggtgaggca gaccgcggtg cgatagctac gattcctctt tacgtacgca atcgtgtcgt
     9901 tggagacaaa tgttggcgaa ccgtacttct ctgactcctc cggatcgccg agcctcacgt
     9961 ctatgccgct atccaggtgg tagacgtgca actgctctct gcgatctacg taggccaggg
    10021 tcttccggtc cggagagatc tcaatggacg aaccggaaaa cggaaggatc tgcaccgttc
    10081 ctgtggagat gtccaccatg ctggtctcgc ctgatttggt ggagcagcag ataatcgacc
    10141 cggcgggagc aacttcggcg atgtcggcag tcgacgaaac ttccattggg cctggcgcct
    10201 gtgttgcaga gattgttcgt atcgtgggca taaccgtcag tgcaaggcaa agtacgtgga
    10261 ggacaagacg agcgtttcta cgcgtgatca tgtgtggatc gcctccgaag agagaatgcg
    10321 ccctttccgg tgtcgatgct caactgcgac cggctacagt atgcggattc ctgtctccat
    10381 aatctcgcgt gctatcgggt tgttctcggt gaagtccgag gttaggaacg gatgcggttc
    10441 gatgcgcgtg tctacctccc gacgcagtct catcagtgca aaagtgtctt ctatcatgtc
    10501 tccagtcaga tcatcagata ccaccgcaat gtcgatatcg ctgtcatcgc catatttgcc
    10561 tgtggcgtag gaaccgaaaa catagagact acgaagagtc agctcccgac gcagcacttc
    10621 ggcataccta cgtccgatgc tcatcagcgc ttcttgagta actctgtcag ccatgcccac
    10681 agctcctcga tctccgccat gcgttcggcg gtgtactccc gagtgcactt gcggtagaac
    10741 gactgctggt agtccggata gcgggcggca acgttgaacg tcgtagtcag atccaacagg
    10801 tccttctgcc gctgggtcat tccgattcca ctcttctccg ctagtagcag taagtcatga
    10861 gaacgaggaa cacttgttcc ctcgggttgg cacgcgataa gtacggcctt cagcaatttc
    10921 tcgatgacca aatgccccat gagcagcgac caatgataat cgccgctatc atagagatgc
    10981 tttacggtct ggtagtctcg attggccgta tccagccagt aggccaccag ctcgtccttc
    11041 tgcattcgga tcaccccctc gagtgcacat tatatctcac gttccgcatg taacccacat
    11101 tcagcccccc gaggggtgcg tgtaacgact gttgctttcg gtgtcgcaga taacccgcat
    11161 tcaggcgtgc aatctcgcca ggtccacagc tgtggctcag ggatataccc ccgggggtat
    11221 ccgcccggag atacggtcac ttttcgacaa tactttcggc gaaaaacgca attcctgagt
    11281 acatcgattt gggccggggg ccccgggggg ccaatcggcc ccgcaaaagg agccctcggg
    11341 ggtcgatatc gccgatttat tggcgaaacg atggcactac acgccattac ctgcgcgccg
    11401 ttaccaaatg aagccatgat gtggtagatt atgtcggcct gcccgcctgc tccaccgcct
    11461 aagcccgtcg gatacccccc gattttcgtg gttaggaggc cgtccggcgg tcctctga
//
LOCUS       DAOTAC010000047        11480 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_4795_length_11480_cov_4.410066, whole genome shotgun sequence.
ACCESSION   DAOTAC010000047 DAOTAC010000000
VERSION     DAOTAC010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 11480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11480
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4795_length_11480_cov_4.410066"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1636..2163
                     /locus_tag="PLM74_03340"
     CDS             1636..2163
                     /locus_tag="PLM74_03340"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HON42151.1"
                     /translation="MAQAARDCRRCKLGGMRKNAVPGEGNPHARIMWIGEGPGADEDE
                     QGRPFVGAAGRMLDRLIKAMGLVREEMFIGNVVKCRPPHNREPEPDEVRACLPYLARQ
                     IELIRPEIIVILGATALKALIDPRAYITRMHGRWIERGGIMIMPTFHPAALLREPAKK
                     HDVWADMQQVLEQMR"
     gene            2249..2650
                     /locus_tag="PLM74_03345"
     CDS             2249..2650
                     /locus_tag="PLM74_03345"
                     /inference="COORDINATES: protein motif:HMM:TIGR04086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HON42152.1"
                     /translation="MKAGSPRRESDRAQSPIWKLVVLGCGVSFLATLVLSAIGGVLTT
                     VFTFSPATIETAIPVIGYLSAGIGGLYVGRKTGERGGRNGLIVGLAYLGFVLLVSAAV
                     IREPMSMASVLTRGASIVILSTLGGIIGVNL"
     gene            2842..3201
                     /locus_tag="PLM74_03350"
     CDS             2842..3201
                     /locus_tag="PLM74_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42153.1"
                     /translation="MIHLAREDVLRGLERFRSIAKQDFLASNLTDNPEFWAAQAAARR
                     EQYDRLIEAIQGSGVPGAVDKAMEWYNELPPLYEEPEFSQPDTRGRKQALEVFFKAVG
                     IDRRTIRDARDQKAARM"
     gene            complement(3298..3915)
                     /gene="lexA"
                     /locus_tag="PLM74_03355"
     CDS             complement(3298..3915)
                     /gene="lexA"
                     /locus_tag="PLM74_03355"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003251517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HON42154.1"
                     /translation="MSSLGAREKQVLEYIARELKVRGYPPSVREIGEAVGLRSSSTVH
                     GYLSSLERKGYIRRDPAKPRAIEVLLGPDQLPPPTREIMNVPIVGRVTAGQPILAVEN
                     VDDYFPLPRSLAGEAESFMLRVSGDSMVGAGILDGDYVIVRKQQAAANGEIVVAMIDD
                     EATVKRLFRADGRVRLEPDNPAYEPIVSDCVAILGKVIGLVRKIH"
     gene            4075..4446
                     /locus_tag="PLM74_03360"
     CDS             4075..4446
                     /locus_tag="PLM74_03360"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HON42155.1"
                     /translation="MQPSHPGSASVTSRRAAYRRRLHRGALCISTLLRVSVAGLLIGI
                     VASVIVWGLSETAPSAQSAEPEQYIVVTVQPGDTLWGIARRVSEGTSDLRVLTNRIKK
                     LNSLDSAMLLPGQSLTVPIGL"
     gene            complement(4641..5459)
                     /locus_tag="PLM74_03365"
     CDS             complement(4641..5459)
                     /locus_tag="PLM74_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012036756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HON42156.1"
                     /translation="MPELPELTILASQMRRELTGKRISRVECFQPKCSNVEPSRLDVT
                     LSGRRVGETTSKGKWLFMPAENGCTLLINLGMGADMVRFVPGGPMPPKYQFRLDLEDG
                     TGFTIRFWWFGYVHLVLNDELQAHAMTRDLGPSPLDPKFTADDFRRLVRRGHGMIKPF
                     LLDQRHVSGIGNAYVHDILFRARIHPRRPIPGLSDAEIDALYASVRDVLGTAIVMGGA
                     YYEKDFYGHPGGYTAEHWLVGYADGRPCTVCGATIRKIKTGSTSSYICPSCQPL"
     gene            complement(5517..5738)
                     /locus_tag="PLM74_03370"
     CDS             complement(5517..5738)
                     /locus_tag="PLM74_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015335161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HON42157.1"
                     /translation="MPIFEYKCKQCSRKFEELVIGCCEKVKCPDCGSEQVDKLLSSFG
                     FKSGSTFASSSSGSSCGSCSGGSCSTCGH"
     gene            complement(5765..6550)
                     /locus_tag="PLM74_03375"
     CDS             complement(5765..6550)
                     /locus_tag="PLM74_03375"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HON42158.1"
                     /translation="MIHVGTSGFSYKDWRGVFYPDSMADSDMLAYYATRFSTVELNFT
                     YYQMPGVRTIEGLCRKTPEGFTFCVKAHSSMTHKVADDEVQVRQDFDKFRAAMQPMAD
                     QGKLGCVLCQFPWGFKRTAKNEAYVQRLPEFLPDWPIVVEYRNVEWVSQATLEALKVA
                     GLGFCCVDEPRLRGLFPPLAVYTSPIAYVRFHGRNAKSWWNHTEASERYNYLYTQDEL
                     NEWIPKIRDLEHSTEHAFVFFNNCHAGQAATNAQMMESLLGLI"
     gene            complement(6578..7030)
                     /locus_tag="PLM74_03380"
     CDS             complement(6578..7030)
                     /locus_tag="PLM74_03380"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HON42159.1"
                     /translation="MLWFGDPPPASEWPEPDSVIVVPMMNRSLLLVHSLRRSGWEFPG
                     GRILAGESPEQAGRREAAEEAGACLGELRTLCWYTVTVWNTLLPGPSRGIVYAADLAS
                     FGSPADEDEVADVRLFSSPPEELSFKDGFTELVFRLMFGTAGAQQTSR"
     gene            complement(7083..8234)
                     /gene="dinB"
                     /locus_tag="PLM74_03385"
     CDS             complement(7083..8234)
                     /gene="dinB"
                     /locus_tag="PLM74_03385"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF010731.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HON42160.1"
                     /translation="MNPKLRTIAHIDMDCFFAAVEALDHPEYRGKPLIVGGQRDSPRA
                     VVSTCSYEARKYGVRSAMPISQAARLCPDALFVTGNMRRYTDVSQQIMECLAEFTPRI
                     QQISIDEAFLDMTGCEHFYNNAQAMGLSIKQQIREKTGLTASCGIAPNKFLAKLASSK
                     SKPDGLLVITADEVGDLLAPLPVRELWGVGERGEQELARYGITTVSDLRAWPAQWLAE
                     RFGKWGMLIYDLARGIDDDPVVTDEECKSYGSESTFDSDVDDIDDLCACLARHAQRVG
                     KRLRAGGKLARTVTLKVKFADFDQITRAKSVSESFDSDDDIYNIACELLRELDMRMAV
                     RLIGVSVSGLTECRQLSLFESPRGKLDRVLDSITDRLGRKGVSRGRELE"
     gene            complement(8270..8851)
                     /locus_tag="PLM74_03390"
     CDS             complement(8270..8851)
                     /locus_tag="PLM74_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HON42161.1"
                     /translation="MSDTVVPVGVSNRHLHVTQDDLEALFGKGYQLRVKKDLSQKGQF
                     AAEETVTLVGPKAAMANVRILGPCRKRTQVEVSRTDCFQLGLNPPVKDSGDLDGSESI
                     KIVGPKGEVTISTGVILAKRHIHMTPEEAEKLGVCDRDVVMVRAPGERALIFDEVLVR
                     VHPTFTWEMHVDTDEANAASLKNGSLVTIIKKD"
     gene            8980..9055
                     /locus_tag="PLM74_03395"
     tRNA            8980..9055
                     /locus_tag="PLM74_03395"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9013..9015,aa:Trp,seq:cca)
     gene            complement(9160..9972)
                     /locus_tag="PLM74_03400"
     CDS             complement(9160..9972)
                     /locus_tag="PLM74_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HON42162.1"
                     /translation="MDKLIITAALVGAELTREQMPHLPVSPTEIAEAAAAAREAGASI
                     VHLHVRDAKGMPTQDVAVFREVITQIKKRTDVICQISTGGAVGTPIELRAEPLSLRPE
                     MATLTTGTVNFGDDVFMNEPSAIEFLASRMKKEGVKPEIECFDAGHMANALRLVKKGL
                     VDEPLYFDFVMGVPGGIPGTVPDLMHCVASLPAGAQWQVAGIGRAELQLAAVAIVLGG
                     NVRVGFEDNIYYSRGVLADSNAQLVERVARISRELGREVATPDEARKLIGLA"
     gene            complement(9976..10374)
                     /locus_tag="PLM74_03405"
     CDS             complement(9976..10374)
                     /locus_tag="PLM74_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009710490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HON42163.1"
                     /translation="MMEDVLIRVRMSQADAHYGGDLVDGARMMQLFGDIATELLIRHD
                     GDEGLFLAYDMVEFRAPVFAGDYIEARGRITRAGNTSRAISFEAFKVIEAGRDETDPS
                     RAYVLEEPVLVARATGTCVVPKDKQRRKGD"
     gene            complement(10394..11170)
                     /locus_tag="PLM74_03410"
     CDS             complement(10394..11170)
                     /locus_tag="PLM74_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OAM dimerization domain-containing protein"
                     /protein_id="HON42164.1"
                     /translation="MPQVDLKSVRPYGDTLDDGRVQLGFTLPIRYGPEAKEAARQLVI
                     KMGLESVDVVSSHDLGGQFTYFVVYGKCTHSVDVTSLRVPKVQVEQMDFFKINDLIRD
                     RLGRKITVVAACTGSDAHTVGIDAIINMKGYAGEYGLERYPWFRAHNLGAQVPNETLV
                     AKAIETDADAILVSQIVTQKNVHISNMTRLVDLLEAEGIRDDVILVAGGPRITHELAL
                     ELGYDAGFGPGTLPSHVAAYIAQELIRKEHRTSPDLGEER"
     gene            complement(11176..>11480)
                     /locus_tag="PLM74_03415"
     CDS             complement(11176..>11480)
                     /locus_tag="PLM74_03415"
                     /inference="COORDINATES: protein motif:HMM:NF020610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysine 5,6-aminomutase subunit alpha"
                     /protein_id="HON42165.1"
                     /translation="KYVFNNARHRGEDIVFKPGGIIRQRAEQVLGDTVRMLEYVRDLG
                     LMEAMGEGVFAGIARTADGGRGLDGVVARSDTYWNPFEEAMSEQLGLAASADPSRR"
BASE COUNT         2301 a         3411 c         3279 g         2489 t
ORIGIN      
        1 cgctacacct cgacctcgcg cccatatgag gcacacccat agtatagagc atgggtctac
       61 gcctgtcaaa cgctttccgg acccctctcg ggacccattt ccggaccatt tccggaccat
      121 ttccgggtgg cttcggggct ctttccaggt ggttcgagcg ccgaactcgc agatatggcg
      181 ctcgggcctt ccgacacttt ccatgaagcc ctgttataca gggcgttctc gctacgcccg
      241 tacgccaagc cgtgttggcc agctaatgaa cgcctcgcga tccatggtag gcaacatata
      301 ctggaaggga ttcgagtctt gctgattggc cgaacacctt gcgtcgggca ggcctttcag
      361 cgaaaccgcg tctagcaagg agtcctctca cctgtaacaa tactgtacgg tttgtgctcc
      421 aacctgctca ggaagtgccc ggttttcacc gggagcaggc aaaactcggg agtttttggc
      481 atttcgtgga ggctctgcgg gcgcctgggg ccttctcggg catattcgag ggatagagtc
      541 atgttaccgg attatgtaaa acgcctctgc cagagcattc agcggcgacg tgattacagg
      601 aaaagaccga gcccctgccg tcgcgaattc agacagttat cacctgccaa accaggcagg
      661 atctgaacag gatgctcgaa aagccagctc aaaaatggac aggatgcccc agaaccccga
      721 tagcccacca caggagatcg tcacgcccag gaacacccat ggacatgggt aatgtgcagg
      781 cctacctggc cttttgtcat gtgttcggtt ttggaacagg ttttcgcgga tgtgtcgcga
      841 atccgaacaa tggttggttc ataacggaac gtatacccgt ataaaagcgg aggatggaat
      901 gattcggtga ccgccacggt aggcaatggg atgatatggc tgctacaggg gtcaggatgc
      961 tagtatatga catcgttagt cagcgccaat gggattatat gttgcgccta cttcgcctga
     1021 ggcgtcttat gcgtctatgc gtccgatcat gcaccaccca ttgttcgttt tggagacaca
     1081 tcgccaaaaa cgtgttcgga aacgggacac cgagattttc ccatcagccc tatggtctgc
     1141 cgctacgctt ttcctactgc cagacttcgc ttcgcgtcgt ctatgaccga aagcccgcgt
     1201 ccccatgggg acgggttcat ttcacttctg gtggggtgcc tgggcatcgt ttctcgccta
     1261 aggcctgcac aaaacgtgtt ttcccatcga ggccctgcat cacactgttc cgccggatga
     1321 cctggcctca gcctcgacag tggtgcgatc cgagggtgca gaggagggca ggggagggca
     1381 gtcaggaaaa tcggcgggga cagcaaggaa ttcgatgcat gtggcataat tgatacaggt
     1441 agtgggggga gacgtaccgt ggatgaggaa aagcaactgc agttgttcga tagcttcgtg
     1501 aatgatgcag tggatgacga gcgtaaaagg cccgtgagct ggcaccgcga tcatgaaccg
     1561 attccggaac cgactctgcg gcgagccttg tggcctgaga ctgatcgccg cgacgactta
     1621 cctgatctgg aggccgtggc tcaagcggcg cgtgactgcc gccggtgcaa gctaggtggt
     1681 atgcgcaaaa atgcggttcc gggtgaaggc aaccctcatg cccgaataat gtggatcggc
     1741 gaaggtccgg gcgctgatga ggacgagcag ggtaggcctt ttgtcggagc ggcgggccgc
     1801 atgctcgata ggctcatcaa ggccatgggc cttgtgcgag aggagatgtt cataggcaac
     1861 gtagtgaaat gccggcctcc tcataaccgt gagccggaac cggacgaggt gcgtgcctgc
     1921 cttccatatc ttgcgcgtca gatcgagctc atcaggccgg agatcatcgt tattttgggc
     1981 gccactgcac ttaaggcgct gatagatccg agggcgtaca taacccgcat gcacggaagg
     2041 tggattgagc gcggcggaat catgatcatg ccgacgttcc atcctgctgc gctccttagg
     2101 gagcctgcca agaagcatga tgtgtgggca gatatgcagc aggttcttga gcagatgaga
     2161 tgatggcctc ggcctgagag acgaatatcc cgaggagcag actaatagtc ttccagtgga
     2221 ggcatgctac cgaggaggtg cgcagtgcat gaaggccggc tctcccaggc gagagagcga
     2281 ccgcgcacag agccccatct ggaagctggt tgtgctcggt tgcggggtca gtttcctcgc
     2341 tacgctggtg ctctcggcaa ttggaggcgt gctcacaacc gtgtttacct tcagccccgc
     2401 cacaatcgaa actgccatac ccgtcatagg atatcttagc gccgggatcg gcggcctata
     2461 tgtgggcaga aagacggggg aaagaggagg gaggaacggc ctcatagtcg gcctggccta
     2521 tcttgggttc gtcctcctag tgtcggcggc agtcatacgt gagccgatga gcatggcgtc
     2581 tgtgctgacg cgaggtgcgt caatcgtgat cttgtctaca cttgggggga taatcggcgt
     2641 taatctgtga gtacgctacg cgcatcaatg cttcgacgat agtcgaggac gtcagagagg
     2701 gcggccggta gttgcaggcc gccctcgtcc gtttcaatgc gggcatacac ttcggcagaa
     2761 ggaatccgcg catcgacagc gaacaagtac ccaaccgaca ggcggtacgt tccgataaaa
     2821 aacgacgggg ggaatggtaa aatgatacac ctggcgcgtg aggacgttct gcgagggctt
     2881 gagcggtttc gcagtatcgc aaagcaggac ttccttgcga gcaacctcac ggacaatcca
     2941 gagttctggg cggctcaagc ggcggcgcga agggaacaat acgataggct gatcgaggcg
     3001 atacaggggt ccggtgtgcc tggggcagtg gacaaggcaa tggaatggta caacgagctt
     3061 ccaccactct atgaggaacc cgagttcagt cagccagata cgcgcggcag gaaacaagct
     3121 cttgaggtct tctttaaggc cgttgggata gatcgcagaa caatacggga tgcccgggat
     3181 cagaaagcgg cgcgtatgta gacgcgtctt tttgtttttg ggccccgaca atgacaagtt
     3241 gaacgtacgg aaacgccggg cggttactct accacccggc gtctggtatt cttgtgctca
     3301 gtggatcttt cgtaccaggc cgattacctt tccgaggata gccacgcagt cgctgactat
     3361 cggttcatag gcgggattat ccggttccag ccttaccctg ccgtctgcgc ggaacagccg
     3421 tttgacagtg gcctcgtcat caatcatggc tacgacgatc tcgccgttgg ctgccgcttg
     3481 ctgcttccgc acgatcacgt aatcgccgtc gagaatgccg gcgcctacca tgctgtcgcc
     3541 cgacaccctg agcatgaagg actccgcttc gcccgctagg gagcggggaa gagggaagta
     3601 gtcgtcgacg ttttcaacgg ccaggatggg ttggcctgcc gttacacgcc ccacaatggg
     3661 cacattcatt atctctctcg tgggaggagg aagttggtca ggccccagca ggacctcgat
     3721 ggctcgcggt ttggccgggt cgcgccggat gtagcccttg cgctctaagg atgataggta
     3781 accatgcact gtggagctgg acctgaggcc taccgcctcg ccgatctccc tgactgacgg
     3841 cgggtatcca cggactttta gttcacgggc aatgtactcg agaacctgct tctccctggc
     3901 gccaagtgac gacaaggcga ccaactcctt tctgccaagc acagtatacc ataggctgca
     3961 tactcctgca aacatgcgtt cgcgaacggc tgttcgtccg gcgcttgaca cggaacatat
     4021 gttctgttat gctgggtcta ccgatagaac atatgtttgc caggagggac cactatgcag
     4081 ccaagccacc ctggttcagc aagcgtcacc agtcgccgcg ccgcctatcg gcgcaggctc
     4141 catcgaggtg ctctgtgcat ctcaacactt cttcgagtgt cggttgccgg gctcctgatt
     4201 gggatcgtgg catcggtcat cgtttggggc ctgtcggaga cagccccgtc tgcgcaatcg
     4261 gctgagcccg agcagtacat cgtggttacg gttcagccag gcgacacctt gtggggtatt
     4321 gcgcgccgag tcagcgaagg cacaagcgac ctgcgcgtgc taacaaaccg aataaagaag
     4381 ctcaattcgc tcgactccgc gatgttgcta ccggggcaga gccttactgt gccgattggc
     4441 ctgtgacgca ccgcctcacc ggggctcgtc ggggttaata ggggttcata aagagaacgt
     4501 cgcataatac acattatgtt aacttagtta tgtgtcacag gcgccggaac gtgcatggac
     4561 ctataacata gacaagcgga aaacatatag ggatatgaag aacatgtgga gatgtagaga
     4621 tgtagagatg cgcagctggc ctacagcggc tggcatgacg ggcatatgta tgacgatgtc
     4681 gatcctgtct tgattttgcg tatcgttgcg ccacaaacgg tgcaaggccg gccgtcggca
     4741 tagcccacta accagtgctc tgctgtatag cctccaggat gaccgtagaa gtccttttcg
     4801 tagtaggcgc ctcccatcac tatggcagtg cccaaaacgt cgcgtacaga ggcgtacagc
     4861 gcgtcgattt ctgcatcgct caagccggga atcggccttc tgggatggat ccgggcgcga
     4921 aacaggatgt cgtggacgta ggcgtttccg atgccggata cgtggcgctg atcgagcaga
     4981 aaaggcttga tcatcccgtg cccgcggcga acgaggcgcc tgaagtcatc agcggtgaat
     5041 tttgggtcga ggggcgacgg tcctaggtcg cgcgtcatgg cgtgcgcctg caactcgtcg
     5101 ttgagtacaa ggtgcacgta gccgaaccac cagaacctga tggtgaaacc agtgccgtcc
     5161 tcaaggtcca gtcgaaactg gtatttgggc ggcataggac ctccgggcac gaaccggacc
     5221 atgtcggccc ccatccccaa gttgatcagc agagtgcaac cgttctcagc cggcatgaag
     5281 agccatttcc ctttgcttgt agtctccccc accctcctgc cagataatgt tacgtcaagc
     5341 cggctgggct cgacgttcga gcatttgggc tgaaagcatt cgacgcgcga gatacgtttg
     5401 cccgtcagct ctcgtctcat ttgacttgct agtatggtga gttcggggag ctcaggcatg
     5461 ggatacacct ccaggctcta taggaaacgg cgccgcgtgc gcggccgact gtaggctcag
     5521 tgcccacagg tgctgcatga tccgccgctg caagaaccgc atgagctgcc gctcgatgat
     5581 gaagcaaagg tcgatccgct cttgaaccca aaggacgaga gcagtttgtc cacttgttca
     5641 cttccacagt cggggcactt caccttttcg caacagccga tcacgagttc ctcgaacttg
     5701 cggctgcact gcttgcactt gtattcgaat atgggcatga tgatcctctc cttactcaac
     5761 tcaatcatat caggccaagt aacgactcca tcatctgagc gttggtcgcg gcttgccctg
     5821 cgtggcagtt gttgaaaaac acgaaagcgt gttcggtgct gtgctcgagg tcgcgtattt
     5881 tcggtatcca ctcattcagc tcatcctgtg tgtacaggta gttgtaacgt tcagaggcct
     5941 cagtatggtt ccaccacgac ttggcgtttc tgccgtggaa cctgacgtac gcaatgggcg
     6001 aggtgtacac ggcaagcggc ggaaagagcc cgcgcagcct gggttcgtct acacaacaga
     6061 agccaaggcc cgcgactttc agggcttcca aagtggcctg cgacacccac tcgacgttgc
     6121 ggtactccac cacaatgggc cagtccggga ggaactccgg cagtctttgc acatacgcct
     6181 cgttcttcgc tgtgcgcttg aaaccccatg ggaactggca caggacgcac cctagcttgc
     6241 cctggtccgc cataggctgc atggccgctc gaaacttgtc aaagtcctgc ctgacttgaa
     6301 cttcatcatc agccaccttg tgcgtcatgc tgctgtgggc tttgacgcag aacgtgaatc
     6361 cttcgggggt cttgcggcac agcccttcta tggtgcggac gcccggcatc tgatagtagg
     6421 tgaagttcag ctcgacggtg gaaaagcggg tcgcgtagta ggctaacatg tcactgtcgg
     6481 ccatgctgtc ggggtagaac actccccgcc aatccttgta gctgaaccct gaagtgccca
     6541 catgtatcat tcagctcacc ccctggtcgc gtcagcgtca gcgcgaggtc tgctgcgctc
     6601 cggcagtgcc gaacatcagc cggaacacaa gttccgtgaa gccgtctttg aacgagagct
     6661 cctcgggtgg cgacgagaat aggcgcacat ccgcgacttc atcttcatca gcaggcgacc
     6721 cgaacgaggc caggtcggct gcgtacacga tcccgcgcga tgggcccggc agcagcgtgt
     6781 tccacactgt caccgtatac cagcacagcg tacgcaactc gccgaggcag gctcctgcct
     6841 cctcagccgc ttcccttctc ccggcctgct cagggctttc gcctgcgagt atccttccgc
     6901 ccgggaactc ccaccccgat ctgcgcagac tgtgcacgag cagcaacgac cggttcatca
     6961 tcggcactac gattacgctg tccggctctg gccattccga agcgggtgga ggatcgccga
     7021 accagagcac tgtatgcact cctgggccat ctaggcaaac tcgggactga ccacccggtg
     7081 gattactcca gttccctacc gcggctcaca cctttcctcc ccaatcgatc agtgatgctg
     7141 tcgagcacgc gatcgagctt gcctcggggc gattcgaaca gcgacagctg cctacattcg
     7201 gtcagccctg acaccgagac tccgattagc ctgacagcca tacgcatgtc gagttcccgc
     7261 aggagctcgc aggcgatgtt gtagatatcg tcatcgctgt caaaggactc actcacgctt
     7321 ttcgcccgcg ttatctggtc aaaatcggcg aacttcacct taagagtgac ggttcgggct
     7381 agctttcccc ctgcgcgaag ccttttgccg acgcgctgtg catgcctggc caggcatgca
     7441 cataggtcgt cgatgtcatc tacgtctgag tcgaaggtgg actctgagcc gtaggacttg
     7501 cactcttcgt cggtcaccac ggggtcgtcg tctatgcctc tagcgaggtc atagatgagc
     7561 attccccact tgccgaagcg ctcagcaagc cactgggcag gccatgcgcg aagatccgac
     7621 accgtagtga tgccgtacct ggccagctcc tgctctcccc tttcccccac tccccacagc
     7681 tctcgcacag gcaggggcgc caggaggtcg ccgacctcat cggccgttat caccaacaac
     7741 ccgtctggct ttgacttcga cgaggccagc ttggccagaa acttgttggg agcaatgccg
     7801 catgacgcgg tcagccctgt cttctcccgt atttgctgct tgatcgagag gcccatagcc
     7861 tgggcgttat tgtagaagtg ttcgcacccc gtcatgtcga ggaaagcctc gtcaatcgag
     7921 atctgctgga ttctgggagt gaactcggct aggcactcca tgatctgctg cgacacgtcc
     7981 gtgtatcttc tcatgttgcc cgtgacgaac agtgcatctg ggcatagccg cgctgcctgc
     8041 gaaataggca tcgccgatct cacgccgtac ttccgcgcct cataagagca tgtcgatacg
     8101 accgcgcgtg gcgagtcgcg ctgccctccg acgatcaggg gtttgcctcg gtactcaggg
     8161 tggtccaggg cttcaactgc ggcgaaaaag cagtccatat cgatgtgagc tatcgtccgc
     8221 aacttggggt tcacctcttg gatcgcccac ctgcctgccc gaccggcgct cagtctttct
     8281 tgattatggt aaccagcgac ccgttcttca gggacgccgc gttggcctca tccgtgtcga
     8341 cgtgcatttc ccaggtgaat gtgggatgca cacgcaccag cacttcatcg aagatcagtg
     8401 ctcgctcgcc cggagcccgg accatgacta catcgcggtc gcaaaccccg agcttctccg
     8461 cttcctcagg ggtcatgtga atgtgccgtt tggccagaat gactcctgtt gatatcgtga
     8521 cttcgccctt ggggccgacg atctttatgg actcagatcc gtccaggtcg cctgaatcct
     8581 tgaccggcgg attgagcccc agttggaagc agtcggtgcg tgatacctcc acctgggtgc
     8641 gcttgcggca cgggcccagt atccgaacgt tggccatagc tgccttgggg ccgacaagcg
     8701 tgacggtctc ttccgctgca aactgaccct tctggcttag gtccttcttc actcggagct
     8761 ggtatccctt tccgaaaagg gcctcgagat cgtcctgtgt gacgtgaagg tgacgattgg
     8821 atactcctac cggaacgacg gtgtccgaca tgactgcgcc tcctgttcta tacaatacaa
     8881 ggctatgatg ccacgattac atgatagccc ttgaggctgc gcttgacaac cgaagacggc
     8941 gactgtagaa tcgaggtagt cggatgatcc ggcgcgtgca ggggagtagc tcaactggta
     9001 gagcatcggt ctccaaaacc gagggttgcg ggttcgagtc ctgtctcccc tgccattctt
     9061 tcctccagaa agtgaacaag ccacctcggc ccagccgggc aaggtggctt tatgctttgc
     9121 acatgactct atctcgccat gcttcttctc ggtttgccgt tacgctagtc cgatgagctt
     9181 ccttgcctca tcgggcgtag ccacctctct gccgagttcg cgtgagatcc tggctacgcg
     9241 ctccaccagc tgggcattgc tatccgccag gaccccgcgg gaatagtaga tgttgtcttc
     9301 aaagcccacg cgaacatttc cgccaaggac tatcgcaact gcagcaagct gcagctctgc
     9361 cctgccgatg ccggcaacct gccactgggc gcctgccggc aggctcgcga cgcagtgcat
     9421 caggtccgga actgtgccgg gtatgccgcc gggcacgccc atgacaaaat cgaagtaaag
     9481 cggttcgtcc actaatccct tcttgaccag ccgcaatgcg ttggccatat gtccggcatc
     9541 gaagcactcg atctccggtt tcacgccctc cttcttcatg cgggaggcca gaaactcaat
     9601 cgcactgggc tcgttcatga acacatcgtc gccgaagttc acggtgccag tggtcagcgt
     9661 ggccatctcg ggcctgaggg agagtggttc agcccgcagc tctatgggcg ttccaacagc
     9721 gcctcccgtg gagatctggc agatcacgtc tgtgcgcttc ttgatctgcg taatgacttc
     9781 gcggaacacg gcaacatcct gcgtgggcat gcctttcgcg tccctcacgt gcagatgaac
     9841 gatggacgcg cctgcttccc tggctgccgc ggccgcctcg gctatctccg tcggcgagac
     9901 cggcagatgc ggcatctgtt cacgcgtgag ttcagcgccg accagagcgg ctgttatgat
     9961 caacttgtcc actcgtcagt ctcccttcct tcgctgcttg tctttgggca caacacacgt
    10021 gcctgttgcc cgggcgacca aaacgggctc ttcgagcaca taggctcgag acgggtcggt
    10081 ctcgtctcgt ccggcctcaa tgaccttgaa agcctcaaac gaaatggcac gcgaggtgtt
    10141 gccggcgcgc gtgatgcgcc cgcgagcctc gatgtagtca cctgcgaaca ccggtgctcg
    10201 aaactccacc atgtcgtaag ccaaaaacag cccctcgtcg ccgtcgtgcc tgatcaagag
    10261 ctcggtggcg atatccccga acaactgcat catcctggct ccgtccacca ggtcgcctcc
    10321 gtaatgggca tctgcctggc tcattctcac cctgatgagg acgtcttcca tcatattctg
    10381 taccgcctcc tttctatcgt tcctcgccca ggtccgggct ggttctatgc tccttcctga
    10441 tcagctcctg agcgatgtag gccgcaacat gcgacggcag tgttccgggg ccgaaaccgg
    10501 catcgtagcc caactcaaga gcaagttcat gcgtgatccg aggcccgccc gcaactagta
    10561 tcacgtcgtc gcggatgccc tccgcttcca gaaggtccac tagtcgcgtc atgttggata
    10621 tgtgaacgtt cttctgcgtt actatctgtg acaccaaaat ggcgtcagcg tcggtctcga
    10681 tcgccttcgc caccagcgtt tcgttgggca cctgcgcgcc gaggttgtgg gcgcgaaacc
    10741 acggatatct ttccagcccg tactcgccgg cgtacccctt catgttgatt attgcatcga
    10801 tgcccaccgt gtgcgcatcg gacccggtgc acgcggccac tacggtgatc ttccggccta
    10861 gtctgtcgcg gattaggtcg ttaatcttga agaaatccat ctgttccacc tggaccttcg
    10921 gcaccctcag cgaagtcacg tccaccgagt gggtgcactt gccgtatacc acgaaatacg
    10981 taaactgtcc tccgaggtca tgcgaactga ccacatccac gctctcaagg cccatcttga
    11041 tcacgagctg gcgggctgcc tccttagcct ccggcccgta cctaatggga agggtgaagc
    11101 ctagctgaac ccttccgtcg tccagagtgt cgccgtaagg cctcacactt ttcaggtcca
    11161 cttgcggcat ggcgctcacc tcctgctcgg atcggcggag gccgcgagcc ctagctgctc
    11221 tgacatggcc tcctcgaatg gattccagta tgtgtccgac cttgccacga ctccgtccag
    11281 ccctcgtccg ccgtccgccg ttcgcgcgat ccctgcgaaa acgccctcgc ccatggcttc
    11341 catcaggccc aggtcgcgta cgtactcgag catgcggacc gtatcgccaa gcacctgctc
    11401 cgcccgttgc cggatgatgc cccccggctt aaagacgatg tcttcgcccc ggtgtctcgc
    11461 gttgttgaac acgtacttgg
//
LOCUS       DAOTAC010000048        11359 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_4875_length_11359_cov_4.408882, whole genome shotgun sequence.
ACCESSION   DAOTAC010000048 DAOTAC010000000
VERSION     DAOTAC010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 11359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11359
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4875_length_11359_cov_4.408882"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2019
                     /locus_tag="PLM74_03420"
     CDS             <1..2019
                     /locus_tag="PLM74_03420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HON42166.1"
                     /translation="MAAGIVAGYLKNAPSVEDVDVEARRVLPTVVYDVNGNVVTKLKI
                     EDRQWVRLADIPQHLIDAVIAVEDHRFYEHHGISVRRILGALWYDIRHLSDDQGGSTI
                     TAQLARNVFLSHEKTISRKIWEALYAFQLERRYTKDEILEFYLNWVYFGHGTYGVQAA
                     SNLFFGKPVDQLTLGEATLLAGIPKGNELYSPIKNEQTRQNGLNRRKIVLSRMVDVGY
                     ITPEQAAQAAEEPLRLASRDNTSAPMGKYVNFMVRDYLLQKYGKDTVYRGGLSVKTTI
                     DLNAQRAAEDAISRLLPTGSTPESRKDNSNLTYPQAALVAIDAQTGQIRALVGGRGED
                     EYNRALRAERSPGSSIKPFVYTTAIDSGYTTTTVVVDEPVEYSAGDGKTWAPRNFNGK
                     FVGALRLRQALEQSTNTVAVKLTEKLGPAKVISYARKMGITTLVDTGPKNDRGLALAL
                     GGLTRGVTPIEMVEAYSPLANSGVKVTPYFILEVRDADGVLLESNVPRREMVLDERTA
                     YIVADMMRGVIYSPNGTGKRADIGRPAAGKTGTADSNTDAWFVGFTPDMVAAVWIGED
                     ANREMRYPNIGVIGSGKAAEIWGAFMKKALAGAPPRDFTVPEGVLTGVRVCTASGELA
                     TPDCPESSVISEKYIEGTQPVHACSVHSGSSPFGVPWQSNAAAPPEEP"
     gene            complement(2038..4518)
                     /locus_tag="PLM74_03425"
     CDS             complement(2038..4518)
                     /locus_tag="PLM74_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HON42167.1"
                     /translation="MKGGEISVDERSLRVLEYYKVRDMLENCVSCGLGRERVRALLPR
                     TQAAAVEEMQSETSEARSALDAGKQIPLGGIRDIRTAVHRASVGAMLGCDELLDVLST
                     LASSRRLKKCLAALGEAYPVLSDAARGIDVPEALERAIEEAISESGEVLDRASPALRS
                     IRSRIRSLNSRVRDTLDSIIRSPQWGKILQDPVVSIRNGRFVVPVRAEMRSHASGIVH
                     DQSGSGATLFIEPMVVVELNNEIRSAVAQEEAEVERILMDLSGRVGAARDRLEMTMAT
                     LGRVDFIMARGKLSQQMGASRPILNSEGSINLIGARHPLLRGDVVPIDPYLGRSFTTL
                     VITGPNTGGKTVTLKTIGLLTLMAQSGLHIPAEPGSEVALFDHVLADIGDEQSIEQSL
                     STFSSHLTHIVEILEQVDENSLVLLDELGAGTDPQEGAGLAMAILEHLHAAGARTVAT
                     THYSELKVFAHTTEGLSNASVEFDVETLRPTFRLSIGVPGASNAFAIAERLGLVGAIL
                     DRARSFVGVGGQRMETMISSLKEEHERASAARSDAEALRARYLNLKDKYEAQVSRSTA
                     ERAEVLRKARVEAELVVTEAKKEADAIIGRLRAAQVEQAARLSTPSGAGQGGVDDEIR
                     GARRDLEALRDLARELTSIDEAPGASSAQETRPEDAIGDDDDSAEMRPGDEVEIVALG
                     QRGRILERGGADEFVVSIGLMRVNAKRDSLRRLRSGGDDRRKGATAGSDSGAVRIESE
                     KARTILTELDLRGMRVDEALDAVDKYLDDAVLSGLPRARIIHGKGTGALRDAVRDQLR
                     QDRRVASIRPGEAGEGGDGVTVASFQKD"
     gene            complement(4515..6737)
                     /locus_tag="PLM74_03430"
     CDS             complement(4515..6737)
                     /locus_tag="PLM74_03430"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3656 domain-containing protein"
                     /protein_id="HON42168.1"
                     /translation="MVSVPGRSSGLHRPRLLVLTGTTAEAFAAVSCGAQAVGFAAGFS
                     LDADPDSRYEGIRRATHYCRVRGVQVYAILNSPANADSAYAAPSMEATVYVSGAECAV
                     ANSLGAFEAARRAFPDWPIWAGWGLAAHNSDSVAALEQAKVSGVILPPELTAGEIAEI
                     AGRTSVQLAAFVHRELCTFYEGHCVSPGVLSPGVGPAECDSWCRRGYTLEAGGAGEPG
                     RRSARRRLLSIKALEALPMLRELVGAGLTWLMVDGAFRGPEHVAIVTGIYSSALDRLA
                     RASGSFAATASEMSLLASASGPELSTGYYERGPGAGIASPKWSGRPKDQATQIIDSDV
                     AAEARARIAAGPPRMPVHMRATARVGGMFLLELADGEGHAVSISGTVPAEAARTTPLS
                     HEYLHRQLSRLGDTPFVLESLQCELDGVAIVPVSDISDVRRRAACALELMRAAPRGER
                     ICDGASMPWAPARGLSSGRTEVAARVSNAQSAAAAAEAGAGLVCIGGEAFVPKVPVGI
                     SEIEGAVLAAHESGAKLYYATSRIIHDREMNAAREGLRRAADIGADGFLVANLGLMDL
                     AAQLTPGAVIADWSFGIIDPFGWSLLSAMGATGFIIPPWLGRDGARLLVHGLGPGAPE
                     MLVFGRVELGVSEYCAVDSVLGGRSGRRACSAPCMSGSFSLRDSGGHIYPVRCDRSCR
                     MHVFDCDELDLIDTLPELGGMGIGSVWLDLRGEPSLRVADVCGEYVPAARRAFGGPRS
                     "
     gene            complement(6792..7421)
                     /locus_tag="PLM74_03435"
     CDS             complement(6792..7421)
                     /locus_tag="PLM74_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42169.1"
                     /translation="MSSNDDGISPIDEEVETVELSREFRERFVRNLQGRDYILYGGVL
                     QLARERGLRRISTSIVQLPSKENSMYAVVEAEIETSDGVFRELGDASPESVSRSIQPH
                     LLRMAATRAKARAMRDAVGIDMVALEELGDAQPAEDFYDEAPAESRAEDFVIRFGKYS
                     GRSLGEILVIDSGYVEWLSNNARDETVRVAAKLLTDAAPEHAATISPAE"
     gene            complement(7405..8103)
                     /locus_tag="PLM74_03440"
     CDS             complement(7405..8103)
                     /locus_tag="PLM74_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HON42170.1"
                     /translation="MLHVIIRAVTVYFVVLAAMRVMGKREIGNLSPFDLVVAIMIAEL
                     GAIPLQEHDIPLLHGLASIVVLAGVQVVVALLCLKYPGVRRAVIGEPTVIIEDGRIMG
                     SNMRKLRYNINDLLSQLRDKNVFNISDVEFAMLEPSGDLSIMLKSQRRPVTPEDLSVP
                     TSYEGPAYPLVADGKIQYKYLKMLDLTVKDVIDRLLEKDVHNLEDVFLASLDTSGELY
                     VTMKDEVSRDELER"
     gene            complement(8190..9926)
                     /gene="polX"
                     /locus_tag="PLM74_03445"
     CDS             complement(8190..9926)
                     /gene="polX"
                     /locus_tag="PLM74_03445"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HON42171.1"
                     /translation="MEPLNQRIAASFDEIADLLELAGDNQFKIRAYRRAAEAVRSLDE
                     PAAEVLRRDQSAVPGIGEALRSKIWEMELSGRMEYLERLRAEIPAAIVVLTQIPGIGP
                     KTARRLYESLGIEDVASLLAAARAERVREIPGFGAKTEANIIKAIQSMQSDGGRKPLY
                     MALPLAEQIVKELLEVNEVEHVDVGGSIRRRRDTIGDIDIIAAASDSGRLMDRFASLD
                     VFCEITSMGHARASAITWSQIRCDLWVVQPESYWTGLHHVTGSKDHHVRLRGIAGKMG
                     LLINERGVYRDSDGQAIAIDSEEAIYSLLGMSYIIPELREDRGEIEAALRGALPAIID
                     RHSIRGDLHTHTSWSDGGASIDDMAKAARAIGYDYFAITDHSRSLGVAHGLSTERILR
                     QIEEVREANARTDGIRILAGAEVDILKDGSLDFPDEILERLDVVVASIHSGFQQDRDT
                     ITGRMVAAMRSPHVDILAHPTGRLLARRPGYEVDIDTVIREAARTGTALEINSYPDRL
                     DLRDEHARAARDAGVMISVSTDSHSPEELPNIEYGVWVARRAWLEPGNVVNAMGYDEL
                     IEWLHTHKASQS"
     gene            complement(9889..10458)
                     /locus_tag="PLM74_03450"
     CDS             complement(9889..10458)
                     /locus_tag="PLM74_03450"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HON42172.1"
                     /translation="MNVSSIDWLSAVLLLMLAADFIVGFRRGLVRQVIDFAGIILAIL
                     AAMRYWRPVAALLERWFAALNPPASNMLGFLAVLIVALVVVDLLGHALSELAKAPGVS
                     LVNGFGGALLRVARGCLLISVVLSLVMSLNLRAVNDAVRSSELAMRLTGFAPSVWKSL
                     DRFIPDDLVIPGLGGSGDGATKPANRGVI"
     gene            complement(10476..10724)
                     /locus_tag="PLM74_03455"
     CDS             complement(10476..10724)
                     /locus_tag="PLM74_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015044023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HON42173.1"
                     /translation="MKDSSRVSALIMGEEYVIRGDAPSEYIEDLARDLDSRMQKLSES
                     NPRLSLHQTAVLSALNLADELSSCRRRYADLLRLLEQV"
     gene            complement(10844..>11359)
                     /locus_tag="PLM74_03460"
     CDS             complement(10844..>11359)
                     /locus_tag="PLM74_03460"
                     /inference="COORDINATES: protein motif:HMM:NF015129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HON42174.1"
                     /translation="CAGLHIDGVTFSRDECPPFHPGRTAAIVRDGVRLGTVGQVHPLV
                     AAGYQIPEATLLFELDMTQLWEAAQCIREYAPLPKYPAVERDVAMVLRRDIASADVER
                     TIRAVGGALVESVRLFDVYEGPQVGEGRRSLAFSITYQAADRTLTDAEVNQVHDAVRV
                     ALARELGAVLR"
BASE COUNT         2279 a         3734 c         3170 g         2176 t
ORIGIN      
        1 atggctgcag gcatagtggc cggctacctc aaaaacgccc ccagcgttga ggatgtcgat
       61 gtcgaagctc gccgggttct gccaacggtg gtatatgacg tgaacggcaa tgtcgtgacc
      121 aagctgaaga tcgaggatcg tcagtgggta cggctggccg atatcccgca gcatctcatc
      181 gatgccgtaa tagccgtaga agaccacagg ttctacgaac accacggtat cagcgttcgc
      241 aggatcctgg gcgcactgtg gtacgacatt cgtcacctat ccgatgatca gggcggtagc
      301 accatcactg cgcaattggc caggaatgta tttctctctc acgaaaagac catcagccgc
      361 aagatctggg aggcgctcta cgctttccag cttgagcgga ggtacacaaa agacgagata
      421 ctggagttct acctcaattg ggtgtacttc ggtcatggca cttacggcgt tcaggcagca
      481 tccaacctgt tcttcggcaa acccgtcgac cagctgactc tgggcgaagc aacgctccta
      541 gccggcattc ccaagggaaa cgagctctac tcgccgataa agaatgagca gaccaggcag
      601 aatggactga atcggcgcaa aatcgtactc agccggatgg tggacgtggg ctatatcacc
      661 cccgaacagg cggcccaagc cgctgaagaa ccgctgcgcc tggcgtctcg tgacaatacg
      721 tcggctccca tgggcaagta cgtcaacttc atggtccgcg actacctgct ccaaaagtac
      781 ggaaaggaca ccgtataccg tggaggtctg agcgtcaaga ctacgataga cctgaacgcg
      841 cagcgtgccg cggaagacgc catatctcgc ctgctgccca cgggcagcac tcccgagtct
      901 cgcaaagaca acagcaacct gacgtaccca caggctgcgc tggtggccat agacgcgcag
      961 acaggccaga tacgagcctt ggtaggaggg cgaggcgagg atgagtataa ccgcgcgctc
     1021 cgtgcggaac gctcgccggg ctcttcgatc aagccctttg tctacactac ggcgatagac
     1081 tcgggctaca ccacgacaac ggtggtagtg gatgaacccg tagaatactc tgcaggcgat
     1141 gggaagacct gggctcccag gaacttcaac ggcaagttcg tcggggctct caggcttcga
     1201 caggcgctgg agcaatcgac gaatactgtg gccgtcaagc tcacggagaa gctgggcccg
     1261 gcgaaggtga tatcttacgc tcggaagatg ggcatcacta cacttgtgga caccggaccc
     1321 aagaacgatc gcggtctggc cctggctctt ggagggctca cccgcggggt gacgcccatc
     1381 gaaatggtag aggcctacag ccctctggcc aactcagggg taaaggtcac accttacttc
     1441 atcctggaag tgcgggatgc cgacggcgta ctgctcgaat ccaacgttcc caggcgtgag
     1501 atggtgctgg atgaacgcac cgcatatatc gtcgccgaca tgatgagagg tgtcatctac
     1561 tcgccaaacg gaacggggaa gcgcgccgat attggacgcc ctgccgcggg caagaccgga
     1621 actgccgaca gcaacacgga tgcctggttc gtgggcttca cgcctgacat ggtggcggct
     1681 gtgtggatcg gcgaagacgc caatcgcgag atgcgttatc ccaatatcgg cgtcataggc
     1741 agcggaaaag ccgccgagat atggggcgct ttcatgaaga aggcccttgc cggggcgcca
     1801 ccgcgggatt tcacggtccc cgaaggcgtg ctgacagggg tgcgcgtgtg caccgcatca
     1861 ggtgagctgg ccacccctga ctgccctgaa tccagcgtca tatccgaaaa gtacatcgaa
     1921 ggcacccagc cggtgcacgc atgcagcgtt cactccggct ctagcccatt cggggttcct
     1981 tggcagagta atgccgcggc accgcctgaa gagccctgat agtgaggacc ggcgtgatca
     2041 gtccttctga aacgatgcga ctgtcacgcc gtcgccgccc tcgcccgcct ctccgggcct
     2101 aatgctagcc acccgtctat cctgccgcag ctgatcgcgg actgcgtccc gtagagctcc
     2161 tgtgcctttg ccatgtatta tccttgcacg cgggaggcca gacagcactg cgtcatcaag
     2221 atacttgtca accgcatcaa gagcctcatc gacgcgcatg cctctcagat ccagctcggt
     2281 caggatggtg cgcgcttttt ccgactctat gcgaactgct ccgctgtctg atccggccgt
     2341 tgcccccttc ctcctgtcat cgccaccgct tcgcagtcgg cgaagggaat cccgcttcgc
     2401 gttcaccctc ataagcccta tactgactac gaactcgtcc gcgccgcccc gctccaagat
     2461 cctaccgcgc tggcccagag cgacaatctc tacctcatcg cccggacgca tctcggcgga
     2521 atcgtcatca tcgccgatcg cgtcttctgg ccgggtctcc tgagcgctgc tggcgcccgg
     2581 cgcctcgtcg atcgatgtaa gctcgcgcgc caggtccctc aaagcctcaa ggtctcgccg
     2641 ggcgccccgg atctcgtcat caacgcctcc ctggcctgcg ccgctcggcg tggacaggcg
     2701 ggccgcctgt tctacctgcg ccgctcggag gcgtccgatt atggcgtcgg cttctttctt
     2761 cgcctcagtg acgaccagtt cagcctcgac tctggccttg cgcaacacct ccgcccgttc
     2821 agcggtggac ctgcttacct gtgcttcgta cttgtctttg aggttcaggt agcgcgcgcg
     2881 aagggcttct gcgtccgacc tcgccgcgct ggcacgctca tgctcttcct taaggctcga
     2941 tatcatcgtc tccatgcgct gcccgccgac tcccacaaaa gatcgtgccc tgtccagtat
     3001 ggcgccgacc agtccgagcc gctcggcaat ggcaaaagcg ttgctggcgc caggcacacc
     3061 gatggacagc cggaaggtgg gcctgagagt ctccacgtca aactccacac tcgcgttgct
     3121 caacccctcg gtggtatgag cgaacacctt cagctcgctg tagtgcgtcg tggccactgt
     3181 gcgggcaccg gcggcgtgca ggtgttccag gatggccatg gccaggccgg cgccttcttg
     3241 tggatccgtg cccgctccca actcgtcgag gagcaccagg ctgttctcgt ccacctgctc
     3301 aagtatctcc acgatatgcg tcaagtgcga ggagaatgtg gacaaactct gctctatgct
     3361 ctgctcatcg ccgatgtcgg cgagcacatg atcgaacagg gcaacctcgc tccccggttc
     3421 ggccggtatg tgcagcccgg actgggccat gagagtcagg agacctattg ttttcagggt
     3481 gacggtcttg cctccggtgt tgggcccggt aatgaccagc gtggtgaaac tcctgccaag
     3541 atacggatcg atgggcacta cgtcgcctcg aagcagaggg tgccttgcgc ctatcaggtt
     3601 aatgctgcct tccgagttca gtatcggcct cgacgcgccc atctgctgcg acagcttgcc
     3661 ccgggccatg atgaaatcta cccggccgag cgtagccatg gtcatctcaa gcctgtcgcg
     3721 ggctgcgccc acgcggcccg aaaggtccat cagtattcgc tcaacctcag cctcctcctg
     3781 ggcaactgct gagcgtatct cgttattcag ttcgactaca accatgggct cgataaatag
     3841 cgtggctcct gagcccgatt ggtcatgtac aatcccggat gcatgcgaac gcatctcggc
     3901 gcgcaccggc accacgaacc gaccgttcct tatgctgacg acgggatcct gaagaatctt
     3961 gccccactgg ggtgaccgga tgatggagtc gagagtatcc cgaacacggc tgtttagaga
     4021 gcgaatcctc gaccgtatgc tacgcagggc agggctcgcc cgatccagca cttcgcctga
     4081 ctcggaaata gcctcctcga tcgcacgctc cagcgcctcc ggaacgtcga tgccccgggc
     4141 ggcatcgctc agcacggggt aggcttcgcc cagagcggcc aggcacttct tcagtcgccg
     4201 gcttgatgcc aaggtagaca gcacgtcgag caattcgtcg cagccgagca tggcgcctac
     4261 agacgctctg tgtactgccg tgcgaatatc gcgaatcccg cctagcggga tctgcttccc
     4321 ggcatccagc gcggaccggg cttcgctcgt ctcgctctgc atttcctcca cagctgcagc
     4381 ttgggttcgg ggaagaagag cccgcacccg ttcccgaccg agcccacagg agacgcagtt
     4441 ttccaacatg tctcgaacct tgtagtactc caagacgcgc agcgaacgct cgtccaccga
     4501 aatctctcca cctttcatga acgcgggcca ccaaaggctc gacgcgctgc cggaacgtac
     4561 tccccacaca catcggccac tcgcaatgag ggctcgcctc tgaggtccaa ccacacactg
     4621 cctatgccca tgccgccgag ttcagggaga gtgtcgataa ggtcaagttc gtcgcagtcg
     4681 aatacgtgca tacggcatga tctgtcgcag cgcacggggt atatatgccc gccgctgtcg
     4741 cggagcgaga aggaaccgct catgcacggc gccgagcagg cacgccgccc cgatctgcct
     4801 cccagcacgc tgtcgaccgc gcagtactcg ctgacgccca actcgacacg tccgaacacg
     4861 agcatctcgg gggcgccggg cccgagccca tgaacgagca ggcgtgcccc gtcccgccca
     4921 agccagggcg ggataatgaa cccagtcgct cccatggcgg aaagcagcga ccatccaaat
     4981 ggatcgatga tgccaaacga ccagtcggct atgaccgcac ccggtgtcag ctgcgccgcg
     5041 aggtccatga ggccaagatt ggcgacgagg aatccatcgg ccccgatatc ggccgcccgc
     5101 cgaagtccct cccgggcagc gttcatctct cggtcatgta ttatccggga tgttgcgtag
     5161 tatagctttg ctccgctctc atgcgcagcc aacaccgcgc cctcaatctc ggagattcca
     5221 accgggactt tcgggacgaa cgcctcaccg cctatgcaca caagaccggc ccctgcttct
     5281 gctgcagcag ctgcgctttg ggcattgctc actctggctg ccacttccgt acgaccggag
     5341 gagagaccgc gggcgggcgc ccatggcatc gacgcgccgt cacagatgcg ttcgccgcgc
     5401 ggcgcagccc tcataagctc cagcgcgcag gcagcccgac gtcgcacgtc gctaatatcg
     5461 ctcacaggaa cgatggccac accgtccagc tcacactgga ggctctccaa cacgaaagga
     5521 gtgtcgccga gccgcgacag ctggcgatgg agatattcgt gtgaaagagg cgtcgttcgc
     5581 gcagcttcag ccggaaccgt gccagatatg ctcacagcat ggccctcgcc gtccgcgagt
     5641 tccagcagaa acatgccccc aaccctcgcg gtcgcacgca tatgcacggg cattcgcgga
     5701 ggcccggcag caatccgggc cctggcctct gccgccacgt ccgaatcgat tatttgcgtg
     5761 gcctgatcct taggcctccc cgaccatttt gggctggcga tgccggcccc cgggccccgc
     5821 tcatagtagc ctgtggatag ctcggggcct gatgcggagg ccaaaagcga catctccgac
     5881 gccgtagcag cgaaggagcc agaagcacgc gccagcctgt ccagggcgga gctgtagatg
     5941 ccggtaacga tcgccacatg ctccggcccc cgaaaagccc cgtccaccat cagccaagtc
     6001 aggccggccc ccaccaattc gcgcagcatg ggcagggcct ccaacgcctt gatgctgagg
     6061 aggcggcgcc tagcgctgcg cctacccggt tcgcctgcac cgcccgcttc cagcgtatac
     6121 ccgcgcctgc accaactgtc gcattcagcg ggtccgacgc ccgggctgag cacgccgggg
     6181 gaaacacagt ggccttcata gaacgtacac agctcacggt ggacaaaggc ggcgagttga
     6241 actgacgtgc gcccggcgat ctccgcgatc tcacccgccg tgagctcggg cggaaggatg
     6301 actcctgaca ccttggcttg ctccagagcg gccacgctgt cgctgttgtg agcagccaaa
     6361 ccccaaccgg cccatatggg ccagtcggga aacgcgcgcc tggcagcctc aaaggcgccc
     6421 agcgagttgg cgacggcgca ttccgccccg gaaacgtaaa ccgtcgcctc catgctgggc
     6481 gcggcatatg ccgaatcggc gtttgccgga ctattcagaa tcgcgtagac ctgtactcca
     6541 cgcacgcggc agtagtgtgt tgcccgacgg atcccctcat aacgtgagtc gggatcagca
     6601 tctaggctga atccggcggc aaagcctacc gcctgcgccc cacacgacac agctgcaaaa
     6661 gcctcggcag tcgtgcctgt gagcacgagc agcctgggcc tatgaagtcc gctcgagcgc
     6721 cctggcactg aaaccatgtc aatcgccccc agggtctggt cgacatgcta cacccaatta
     6781 tagcagaacc gtcactcggc aggcgagatc gttgcagcgt gctccggggc cgcatccgtc
     6841 agcagcttcg ccgccacccg caccgtttcg tctcgggcat tgttgctcag ccattcgacg
     6901 taacccgagt cgatcacgag tatctcccct agcgaccggc cggagtactt cccgaacctg
     6961 atcacgaagt cctccgcgcg gctctccgcc ggcgcttcgt cgtagaagtc ctcagctggt
     7021 tgagcatctc ccagttcctc gagggccacc atgtcgatcc caacagcatc cctcatcgcc
     7081 ctggccttgg cccgcgtggc agccattctc agaaggtgcg gttggatcga ccgtgacacg
     7141 ctctcaggag acgcgtcgcc tagctcgcgg aagactccat cagatgtctc aatctcagcc
     7201 tctaccaccg catacataga gttctcttta gagggcagct gaacaatgct ggtgctgata
     7261 cgccttagcc cgcgctccct ggctagctgg agcacgcccc cgtaaaggat gtaatcacgt
     7321 ccctggaggt tccgcacgaa tcgttcacga aactctcgtg aaagctccac tgtctccacc
     7381 tcctcgtcta tcgggctgat gccgtcatcg ttcgagctca tcccgggata cctcgtcttt
     7441 catagtaacg tacagttcgc ctgatgtgtc gaggctggcc agaaagacat cctccaggtt
     7501 gtggacgtcc ttctccagaa gcctgtcgat gacatccttc acagtcaagt cgagcatctt
     7561 gagatactta tactggatct tcccgtccgc cactaatggg taggcgggac cctcgtacga
     7621 tgtgggcact gacaaatcct caggcgtcac cggtcgccgc tgcgacttga gcatgatgct
     7681 cagatctccg gatggctcca acatggcgaa ctccacatca gagatgttga agacgttctt
     7741 gtcccgaagc tgactcagca gatcattgat attgtaccga agcttcctca tgttacttcc
     7801 cataattctt ccgtcctcaa tgatcacggt gggttcgcct atgaccgctc ggcgcactcc
     7861 ggggtacttt aggcacagca aggccactac cacctgaact ccggccaaga ccacgatgga
     7921 cgccagcccg tgcaggagcg ggatatcgtg ctcctgcagc ggaatggctc cgagttccgc
     7981 tatcatgatg gccactacca gatcgaacgg tgagaggttg ccgatctccc gcttccccat
     8041 cacacgcatg gcagccagga caacaaagta gacagtgact gcccggatta ttacgtgcag
     8101 catgtcggcc tccagattcg ccaaaaatca gcgcgtggcg cataccgtag tatgcccacg
     8161 cgcgaattcg agctatgccg accggcccgt cagctctgag aagccttgtg cgtgtgcaac
     8221 cactcaatca gctcatcgta gcccatggca ttgactacat tgcccggttc cagccaggcc
     8281 ctccgggcca cccacactcc gtactcgatg ttgggaagtt cctccgggga gtgcgaatct
     8341 gtgctcacgg atatcatcac cccggcgtcg cgcgccgccc ttgcgtgctc gtcgcgcaag
     8401 tcgagccggt cggggtatga gtttatctcc aaggcagtgc ctgtgcgtgc agcctcacgg
     8461 atcaccgtgt cgatatccac ctcgtagccc gggcgcctcg ccagcaatct gcctgtggga
     8521 tgagccagta tgtccacatg cgggcttcgc atcgcagcga ccatcctgcc ggtaatcgta
     8581 tcgcggtcct gctgaaaacc gctgtgtatc gatgccacaa ccacgtccag ccgctccaga
     8641 atctcatcgg ggaagtccag actgccgtct ttcaatatgt ccacctcagc gccggccagg
     8701 atccggatcc cgtctgtacg cgcattggcc tctcgcactt cctcgatctg ccggagaatc
     8761 ctctcggtgg acaggccgtg tgctacgccc agcgaacggg agtgatcggt gatggcgaag
     8821 tagtcgtacc caatggcacg cgcggcctta gccatgtcgt ctatagatgc tccgccatcg
     8881 ctccagctgg tgtgtgtatg cagatcgcct cggatggaat gcctatctat gatcgcgggc
     8941 aaggccccgc gcagcgcagc ctcaatctcg cctctgtctt cgcgcagttc cggaatgatg
     9001 tagctcatcc cgagaagaga gtagatcgct tcttcgctgt caatggcgat ggcctgcccg
     9061 tccgaatcgc ggtagacccc tcgctcgttg atgaggaggc ccatcttgcc agcaatgccc
     9121 ctgagtctca catgatgatc tttggagcct gtcacgtggt ggagaccggt ccagtacgac
     9181 tccggctgca ctacccacag atcgcatcgg atttgcgacc aagtgatggc gctagccctt
     9241 gcatgtccca ttgatgtgat ctcgcagaac acgtccagcg acgcgaacct gtccatcagc
     9301 cgcccgctat cggacgcagc agctatgatg tcgatatcgc cgatggtatc gcgcctccgc
     9361 ctaatgctcc cgcccacgtc gacatgctcc acttcattta cttcaagtag ttccttgacg
     9421 atctgttcgg caaggggcag ggccatatag aggggtttgc gtccgccgtc tgactgcatg
     9481 gattgaatgg ccttgatgat attcgcctcc gtctttgcgc cgaaacctgg tatctcccta
     9541 actctctctg cccgcgctgc cgccagcagg cttgccacat cctcgatgcc cagggattcg
     9601 taaagtcgcc gggcagtttt cggcccgatc ccaggaatct gagtcagcac tacaatagcc
     9661 gcgggtatct cagcacgtag acgctccaga tactccatcc ggccgctcaa ttccatttcc
     9721 catatcttcg accgaagggc ttcgccgatg ccgggaacgg ccgattgatc acgcctgagc
     9781 acttcggctg ctggttcatc cagcgacctg acagcctccg ccgctcgcct gtaggctctg
     9841 atcttgaatt ggttgtcgcc tgccagttcc agaaggtcgg caatctcgtc aaatgacgcc
     9901 gcgattcgct ggtttagtgg ctccatctcc cgatccccca agcccgggta tgaccagatc
     9961 atccgggatg aacctgtcca gagacttcca gacggacggt gcgaatcccg tcagcctcat
    10021 cgccaactcg gatgaacgta cagcatcgtt cacggccctc aggttgagcg acatgacaag
    10081 cgagagaacc acactaatga gcagacaccc cctggccacc ctgagcaggg cgccgccgaa
    10141 tccgttgacc agcgatactc caggcgcctt cgcaagctcc gaaagggcgt gcccaagcag
    10201 gtccaccaca accagcgcga cgattaacac cgcaaggaaa ccaagcatgt tcgatgcggg
    10261 cggattgagc gcggcaaacc acctctccag gagagctgcg acaggcctcc agtatctcat
    10321 cgccgcgagg atcgctagta ttatgcccgc gaagtcaatc acctgacgca ccagtcctcg
    10381 gcggaaaccg acgatgaagt cggcggccaa catcaggagc aagactgcgc tgagccagtc
    10441 tatggaggag acgttcattg gaatccctcc ccgaactaca cctgctcgag caatctgaga
    10501 agatcggcgt agcgccggcg gcacgatgag agctcatcgg ctaagttgag ggctgagagc
    10561 accgccgtct ggtgcaacga aagcctggga ttggactctg acagtttctg catacgcgag
    10621 tccaagtcgc gcgcgagatc ctcgatgtac tcgctgggcg catctccgcg tatgacatac
    10681 tcctcgccca ttatcaaagc gcttactctc gagctatcct tcatgagatg accgctcccc
    10741 tcgacgctat gatgcgggat cgagcccgcc gccatctatg agagtttgcc aaacgtacgc
    10801 tatatcctgc aatgtgcgcc ccccagcccc agcggcgaca gccctatcga agcacggcgc
    10861 ctaactcccg agcgagagcc acccgaaccg catcatgcac ctggttcacc tctgcatccg
    10921 tcagcgtacg gtccgcggcc tggtaagtga tggagaacgc caggcttctc ctgccctctc
    10981 caacctgtgg accctcatac acatcgaaca accttacgga ctccacaagg gcgcctccca
    11041 cagcgcggat ggtgcgctcg acatccgctg acgctatatc gcgcctcaga accatagcga
    11101 catcgcgctc gaccgcaggg tattttggca gcggcgcata ctcccgtatg cactgcgcgg
    11161 cttcccaaag ctgcgtcata tccagctcaa acagcagggt agcctcgggt atttggtatc
    11221 cggcagcgac gagaggatgc acttgcccga ctgtgcccag ccgcacaccg tcgcgtacta
    11281 tggcggcagt ccggccggga tggaatggcg gacactcgtc acgactgaag gttacgccgt
    11341 caatgtgcag accagcaca
//
LOCUS       DAOTAC010000049        11279 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_4916_length_11279_cov_4.468550, whole genome shotgun sequence.
ACCESSION   DAOTAC010000049 DAOTAC010000000
VERSION     DAOTAC010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 11279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11279
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4916_length_11279_cov_4.468550"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            666..1961
                     /locus_tag="PLM74_03465"
     CDS             666..1961
                     /locus_tag="PLM74_03465"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HON42175.1"
                     /translation="MKNRTLRTGDRRLIRDINMSLVVNSIRQHGPISRVDVARATGLG
                     RSTVSGIVSLLLKEEFVREIGSGHTDSTSPAVGRPPVMLEFNPRSRFVVAVKLAPASI
                     TAAVTDLNADVVGLVERPINARLSEGAVRQQISEAVYAVVSECRVSMDQVIGVGVAVP
                     GIVDSRTGVSISPQFFLWQNLPLRNLLEEEFSVPVFIDNDANAATLAEKLRGHGRQAS
                     DFVCVTIGIGIGAGIIIDNKLYRGAVAGAGEIGHITIQEDGPACTCGNHGCLEAFASD
                     AALVREMKRIVESGRSSAVTRISAEESRDLDRPMIARAAREGDDAALSVVRHAGEHIG
                     VGLATLVNILNPEMIVVAGEAAIDFGDLLLEPIRDSIRRRSVSVLAQRLEVVESLLGD
                     RIWIVGAASLVIDDFFGAPIYERHDHVAAVSIPDLMQGS"
     gene            2049..3224
                     /locus_tag="PLM74_03470"
     CDS             2049..3224
                     /locus_tag="PLM74_03470"
                     /inference="COORDINATES: protein motif:HMM:NF012926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HON42176.1"
                     /translation="MHDTLVDASISLITNNQHASGAYIASPSFPTYAYCWLRDGTYTA
                     HAMDLYGEHESAKAFFRWVNATISSNRADTERTSDAIEDAEPEGRRRAAQSVEQARRL
                     LRARWTLDGKRDNSEWPNYQPDGYGAWLWGLDEHIERTGDLGLWHESREAVGYVVECL
                     SQVWSTPSSDCWEEYPDRIHTSTLICIAGGLERIGRRASDDRALSLAAAIRDYIIRYG
                     VFDGRLAKLCSHERRGDQWPPKRRTHAGPSIGDSVDASLLWAAVPYGVLDPADPRMVS
                     TVSAIERGLVSGGGVRRYAADTYYGGGLWPLLTAWLGWYYCRTGDREKALGCLRWVEN
                     QATDGVHLPEQVNRGLVDPVLYEPWVTCWGPPANPLLWSHAMYLILRSELDDRAREV"
     gene            3230..4114
                     /locus_tag="PLM74_03475"
     CDS             3230..4114
                     /locus_tag="PLM74_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007488313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HON42177.1"
                     /translation="MYIGKGRKRQATIAAFLAPTVAGFLLFSLIPIARSLYLSFTSWD
                     ILQPAEWVGLENYKVLLRSEEFWRVMRNTGEYIVLYVPLMLVSSLALAALLNRRFAGV
                     GVYKVLFFLPVLTSWVAGSLIWRWILNHKYGLVNILLGHIGIAGPAWLDDKYWAMPGI
                     VLASVWKDAGFFALIFLGALKGINHEYYEAAQIDGASPWKRFRHITLPLVSPTTFFVT
                     ITSIINSFQVFAQVQIMTYGGPAGATQVIMERIYKYAFQFFKMGYASALSWVLFVIIL
                     IITVIQWRLQKRWVFYDG"
     gene            4104..4946
                     /locus_tag="PLM74_03480"
     CDS             4104..4946
                     /locus_tag="PLM74_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015256139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HON42178.1"
                     /translation="MMAKRAGAFRKVLFYVTISLVALFMILPFLWMISTSFKESEAVM
                     VLPIRWIPERISLKAYRDLFTISTVVPFHRATLNSLAVSVLTTFVNLASASMAAFALA
                     KFDFKARDKVLFAFLATMMVPGAVTMIPNYLVLRTLRLTNTFTGLVLPSIYNAWALFL
                     LRQNIMALPEPYFEAAIIDGATPFAVYRHVVLPMVKPILASLGVLTFMGTWNDYLWPL
                     IMISDPEKMTLTLALGNLNSRWGQKWELLMAGNLISMLPIIMTYVFAQKYFESGISVG
                     GLKS"
     gene            5088..6338
                     /locus_tag="PLM74_03485"
     CDS             5088..6338
                     /locus_tag="PLM74_03485"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HON42179.1"
                     /translation="MHKKRLALAAVCVALVALCAMPVFGAKKVTITYGNFSAGSDNEP
                     TLNAMKAAFEKANPDIEIKLQSTGFGEYFRLLQTQVAGGTAPDCYELNYENFVAFAAK
                     NALADLGPLAKAAGVSFDGYDANALDAFKYKGKQYGVPGSYSVVLLFYNKDLFDRAKV
                     AYPNDNWTWKDVIESGKKIRALGDDTFGIIQTVQFWEFYKTIAQNGGSLFNADRTKFT
                     VNSPENVEALQYMVDKIIKYNIEPTQAQKSNQGEWDMFAAGKVGMLITGNWAFTYMKD
                     KCKFRWDIAVEPGNKQKACHFFSNGYAINKSSKKQQEAMRWITWLASSKESSMLRLEA
                     GWETPPVSDKSVIDAYIAERPPDNRKAVFDSLKYLVTPPVIEQFNEMAEIVDLQLQKA
                     RDGEKTPKQALDDAQRELEQKIKL"
     gene            6491..8902
                     /locus_tag="PLM74_03490"
     CDS             6491..8902
                     /locus_tag="PLM74_03490"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HON42180.1"
                     /translation="MPHHDEFRVCAEYACGSGARLRLTDGFVTAQFDNVGQLVPRVEF
                     FFRDGRLHITHSLATEPMNVGDLESRACIVDPDTGAKLEVESNPFRFTLVSADGRTLL
                     HNASGEPHFKFRAAPDGEMFQFTLSLGSSASHYYGFGERFDSLDQNGLNPDVCVVNQY
                     CRQGSRTYIPMPWFVTEVGYGIHVVTDRYVRFGLAPRLPGTMVIEAQIDPGRPVLESA
                     VFLGTPADIVRAYARSTGATALPPKWAFGPWMSGNAWNTQRDVEEQLRVSDELGLPAT
                     VVVIEAWSDEATFYIWNDAHHAPESGEYGFRLDEFTFPAEGKWPDPKALADHIHDSGM
                     KLVLWQIPVLKRLAAHEPGCAQHERDEEHAIARGYVVRNADGTPYRIPDGWFAGSLLL
                     DFTNPEATEWWFSKRRYLIDQLGVDGFKTDGGEFIFDDRVSFWDGRNGASMRNRYAMT
                     YIEAYRDFAGPGRITFSRAGHAGAQSSGLFWAGDQESSFAESRAVLTAGLSIGLSGVP
                     FWGFDMAGLSGEIPTAELFIRALEMAAFSPVMQYHSDGPAAWDRTPWNIAARTGDERV
                     VSTYRTYANLRMNLLPYIYNEAVSSAKSSEPLMRALMIDFPCDPASFSIDDEYMFGRD
                     LLVAPVLHEHARERSVHLPPGQWLDFWTFEEIGTGPCTMPSYPSDIGKIPVFVRAGAI
                     LPINLGDSMHLGSTTGVRGVGYRNLSFLITGVPAQEWVFTDDEGTRIRFVPGDGGLRV
                     VADYAGAVDHVNLLILGHGGHGQRERADRQCRTVWAAGGRRAEVVRVGTESLIQGILL
                     PLLLP"
     gene            9145..10440
                     /locus_tag="PLM74_03495"
     CDS             9145..10440
                     /locus_tag="PLM74_03495"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HON42181.1"
                     /translation="MSRLERTNGRERSTQMSTDMMYPNPEADARAEAQGFDARRLRHA
                     VDLVKRRCAGESDHPSYPGAVLLIMRHGVEVVHEAFGFRSYDESAEPMTRDTIFDVAS
                     MSKPIATATAVLRLVETGEVNLDDPIARYIPGVGIAADGAGAGAGAGAGAGADITLMN
                     LLTHTSGLPPSTTVFKGAESRDDCVRIARSVLPEYAVGSKVEYSCMGFILLALLVEAV
                     AGESLNVFARREVFDKLGMKDTGYYTVERFAERARVAPTESRTSCEKGEELWAWLNKT
                     GQFLDLHTRRQVAWGAVHDENALAMGGVSGNAGVFSTAADIARFGQMYLQHGCYMGAR
                     VLSPATVELATRNFTAGMGDNRGLGWQLKGLNTSFGSLISDRAYGHTGFTGTVLWIDP
                     DRELIVVLLTNRVHETRENQALVDLRPRFLNAVVAAISR"
     gene            11009..>11279
                     /locus_tag="PLM74_03500"
     CDS             11009..>11279
                     /locus_tag="PLM74_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HON42182.1"
                     /translation="MKTLEPIGDGQSVPSRAYERIMEFIAEGDLVGGKLPPETVLAQQ
                     LGVSRTALREALQRLESEGYIVRRRRVGTIVLASRLKLDAGLERLN"
BASE COUNT         2413 a         3008 c         3495 g         2363 t
ORIGIN      
        1 ccggagagat ctaccttgtt taccaccagg gcgatgaggc gctggtgcag ctagatgccg
       61 accacatgtt cgactgcctg gccagactgg agaaggtcga cgaagcgatt tgcgccaccg
      121 tgacgtggct ggacaagacg ctttaggctg gcaagctgac ctgctgaaag ccagagacac
      181 acctggagcc gaggctgaat acctcggctt tctgtttggg tgtgtctggc atgatcgccg
      241 cacatacagg cttccatatg gtcgcgtaaa ggactgctca gcccgttttt ccggctcgcg
      301 cgctgtgtat ggccttcttg gcggggtgtt gcagctgcat accgtgtcgc gccgagagcc
      361 gtgcagccag gcgcctgaca gagccgtgtc ctgctcagaa gccgtagata ctgcagaatg
      421 gataggtctg gttagtctgg tacctcagct gcagaagttt cgtctggccg aagaaggatt
      481 tccgcccaat gagtagaact caacccacgc agagttaata cggtcatcga acaaactaat
      541 accgtaactc cataaaacgg gattaccagc gataatgaac ccatattaca tcaatagtgc
      601 attcatagac cataatagcg tgatcgcgac tatattgatg tatgggaaga gaggcatggt
      661 cgtctttgaa gaatcggact ctgagaacag gtgacaggcg gctgattcgc gacattaaca
      721 tgtcgctagt cgtgaactcg ataaggcagc acgggcccat atcccgcgtg gatgtagcgc
      781 gcgcgactgg gctgggaaga tcaaccgtat cgggaatcgt gagcctattg ctcaaggagg
      841 aatttgtgcg cgagatcggc agcggtcata ccgactccac ttcgcccgcc gtgggccgac
      901 ccccggtaat gctggagttc aaccctcgtt ccaggttcgt ggtagcggtg aaacttgctc
      961 ctgcgtcgat cacggctgct gtgaccgacc ttaacgccga tgtggttgga cttgtggaaa
     1021 ggccgataaa cgccaggctg agcgaggggg ctgtaaggca gcagatatcc gaagccgtat
     1081 acgcagtcgt ctccgaatgc cgggttagta tggaccaagt cattggcgtg ggtgtggctg
     1141 tgccgggcat cgtggattcg cgtaccggcg tgtcgatctc gcctcagttc ttcctctggc
     1201 agaaccttcc tttgcggaac ttgctggagg aggagttcag tgtgcccgtg ttcatcgaca
     1261 acgacgcgaa cgccgccact ttggcagaga agctgagggg gcacggtagg caagcatcgg
     1321 atttcgtgtg cgtgacgatc ggcatcggta ttggcgcagg catcatcatc gataacaagc
     1381 tgtaccgggg cgccgttgcc ggagcaggcg agatagggca catcacaatt caggaagacg
     1441 gccctgcgtg cacctgcgga aatcatggct gccttgaggc gtttgcgtcc gacgccgccc
     1501 ttgtgcgcga gatgaagcga atagtcgagt cgggccgtag cagcgccgtc actcgaattt
     1561 ctgcggagga gagccgggac ctcgacaggc cgatgatcgc tcgtgccgcc agagaagggg
     1621 atgatgcggc tttgtcagtg gtgaggcatg ctggtgaaca tattggagtc ggtctggcga
     1681 cattggtgaa catcctcaac cctgagatga tcgtggttgc aggcgaggct gccattgact
     1741 ttggcgatct cctcctcgaa cccatacgtg attccatccg tcgccgttct gtgagcgttc
     1801 tcgcccagag gctagaggtc gtcgaatcgc ttctcggcga caggatatgg attgtgggcg
     1861 ctgcctcgct ggtcatcgac gatttcttcg gagcgcccat atatgaacgg catgaccacg
     1921 tggcggcagt atcgattcca gacctgatgc aaggctcata gatcagaccc caccagttgg
     1981 cgagcgagac agtaatcgga gcacggcagg ggatgctcca atgacaattc cggaacggga
     2041 gatttgtgat gcacgacacg ttagtagacg caagcatttc gctgataacg aataaccaac
     2101 acgcttctgg ggcgtacata gcctcgccca gcttcccgac ttacgcatac tgctggctcc
     2161 gcgacggaac ctataccgct catgctatgg atctttacgg tgagcatgag agcgcgaagg
     2221 cctttttccg ctgggtgaac gcgaccattt caagcaaccg tgccgacact gagcgcacct
     2281 cggatgccat cgaagacgcc gagcccgaag gacggcggcg agccgcacaa tcggtcgaac
     2341 aagcacggcg gttgctcaga gcacgatgga ctttggatgg caagcgcgac aactccgaat
     2401 ggccaaacta ccagccagac gggtatgggg catggttatg ggggcttgac gagcatattg
     2461 agcgcactgg agaccttggc ttgtggcatg agtcgcgcga ggccgttggg tacgtcgtgg
     2521 agtgcttgtc gcaggtgtgg tcgacgccta gcagcgattg ctgggaggag tatcccgata
     2581 ggattcacac atccactctg atctgcatag ctggcggcct tgagagaatc ggtcgccggg
     2641 cgtccgatga tcgggctctc tcattggccg ccgcaatccg ggactacatc attcgctacg
     2701 gtgtgttcga cgggcgcctt gcgaagctct gcagccatga gcgacgtgga gatcaatggc
     2761 ctcccaagag gcgtactcac gcggggccat ccatcggcga ttcagtcgat gcaagcttac
     2821 tctgggccgc agtgccctat ggagtgcttg accccgccga tccgcgcatg gtgagcacag
     2881 tcagcgcgat cgagcggggt ctggtttcgg gaggcggagt gcgccgctac gcagccgaca
     2941 cctactacgg cggcggtctg tggccgctcc ttaccgcgtg gctgggctgg tactactgcc
     3001 ggacgggcga cagggagaag gcgctggggt gcctcaggtg ggttgagaat caggctaccg
     3061 atggggttca cctgccggaa caggtgaacc ggggtcttgt cgatcctgtg ttgtatgagc
     3121 catgggtcac atgctggggg ccgccggcga atccgcttct ctggtcacat gcaatgtatc
     3181 tcattcttcg tagtgagctc gatgatcgcg cgcgggaggt gtagggcgta tgtacatcgg
     3241 caaggggagg aagcggcagg ccactatcgc ggcattcttg gcgccgactg tagcagggtt
     3301 tctccttttc agcctgatcc caatagcccg atcgctttac ctcagcttta cgtcatggga
     3361 tattctgcag cctgccgaat gggtgggact ggagaactac aaggtgctgc tgcgcagcga
     3421 agagttctgg agggtgatga gaaacacggg ggagtacata gtcctctatg ttcctctgat
     3481 gctcgtatcg tcgcttgccc tggcggcgct cctgaacagg aggttcgccg gagttggcgt
     3541 gtacaaggtg ctgtttttcc ttccggtctt gacatcctgg gtggccggtt cgctgatctg
     3601 gaggtggatt ctcaaccaca agtacggcct agtgaacatc ttgctcgggc acattggcat
     3661 tgcgggcccg gcatggctcg atgataagta ctgggcgatg ccgggaatcg ttcttgcgag
     3721 cgtatggaag gacgcagggt ttttcgccct gatattcctg ggcgcgctca agggcattaa
     3781 ccacgagtac tacgaagccg cccagatcga tggcgcaagt ccatggaaga ggttcaggca
     3841 cataactctg ccactcgtgt cgcctaccac gttctttgtg accattacgt ccatcatcaa
     3901 ctctttccag gtattcgccc aggtgcagat catgacgtat ggagggcctg ccggcgctac
     3961 tcaagtcata atggagagaa tctacaagta cgccttccaa ttcttcaaga tgggctatgc
     4021 atcggccctc tcgtgggtgc tgttcgtaat aatactcata atcaccgtga tccagtggcg
     4081 cctccagaag aggtgggtgt tctatgatgg ctaagcgagc gggcgcattt cgtaaggttc
     4141 tgttctacgt caccatcagt ctggttgctc tgttcatgat actcccgttt ctgtggatga
     4201 tcagcacatc gttcaaagag agcgaggcag ttatggtgct gccgataagg tggatacctg
     4261 agagaatcag cctcaaggca taccgtgacc tgttcacgat atcgacggta gtgccattcc
     4321 accgggccac gctgaactcg ctggcggtat cggtgctcac cacctttgtg aatctggcgt
     4381 cggcgtctat ggctgcattc gctctggcga agttcgattt caaggcgcgc gacaaagttc
     4441 tattcgcttt tctggccacg atgatggttc ctggggcggt cactatgatc ccgaactacc
     4501 tggtactgcg cactcttcgc ctgaccaaca cgttcaccgg acttgtgctg ccctcgatat
     4561 ataacgcctg ggcgctgttc ctgctgaggc agaacataat ggcgctgccg gaaccgtact
     4621 ttgaggccgc gataatcgac ggagcgacgc ccttcgccgt gtataggcat gtggttctgc
     4681 ctatggtgaa gccgattctg gcatccctgg gagtgctgac gtttatgggg acctggaacg
     4741 actacctctg gcctctgatc atgatatccg accccgagaa gatgactctc actcttgccc
     4801 tgggcaacct gaactcaaga tgggggcaga agtgggagtt gcttatggcc gggaatctca
     4861 tttcgatgct gccgataatt atgacttatg tctttgccca gaaatacttc gaaagcggga
     4921 tctctgttgg ggggctcaaa tcctagaaaa gtgccgggag tgaaagaagg tgagctcggg
     4981 taacgggcaa cgcaacgaca tgagtggaga ctgttggagt attgtcggac tgaccataat
     5041 ggcgtggcgg ctttccacta tctactcact actagggagg gatacgcgtg cacaagaaga
     5101 gacttgccct ggcggcagtg tgtgtcgccc tcgttgctct gtgcgcaatg ccggtctttg
     5161 gcgcgaagaa ggtaaccata acctacggca acttctccgc gggatccgac aacgaaccta
     5221 cgctcaacgc gatgaaggcg gcgtttgaga aggccaaccc agatatcgag atcaagcttc
     5281 agagcactgg attcggcgag tacttcaggc tcttgcagac tcaggttgca ggagggacag
     5341 cgccggactg ctacgaactg aactacgaga acttcgttgc cttcgcggcc aagaacgccc
     5401 ttgccgacct cggtccactg gccaaggccg ccggagtcag ctttgatggg tacgacgcca
     5461 acgcgcttga tgctttcaag tacaaaggaa agcagtatgg agtgcccgga agctactctg
     5521 tagtcctcct tttctacaac aaggatctct tcgacagggc caaggtggcc tatcccaacg
     5581 acaactggac gtggaaagac gtaatcgagt ccgggaagaa gattcgggcg ctcggagacg
     5641 acaccttcgg gatcatccag actgtgcagt tctgggagtt ctacaagact attgctcaga
     5701 acggcggcag cctgttcaat gccgatcgca ccaagttcac agtgaactcg cctgagaatg
     5761 tggaagcact gcagtacatg gtcgataaga ttatcaagta caatatcgag cccacccagg
     5821 cacagaagtc caaccagggc gagtgggaca tgttcgccgc cggtaaagtc ggaatgctta
     5881 taacgggcaa ctgggcattc acttacatga aggacaagtg caagttcagg tgggacattg
     5941 ctgtggagcc cggaaacaag cagaaagcat gccacttctt ctcgaacggg tatgccatca
     6001 acaagtccag caagaagcag caggaagcca tgagatggat aacatggctg gcgtccagca
     6061 aggaatcgtc aatgctgagg ctggaggcag ggtgggagac tccgcccgta tctgacaaga
     6121 gcgtcatcga tgcgtacatc gcggagcgtc cgccggacaa tcgcaaggca gtctttgact
     6181 ccctgaagta tctggtgacg ccgcctgtaa tcgagcagtt caacgagatg gcggagatag
     6241 tcgatctcca actgcagaag gctcgggatg gggagaagac ccccaagcag gcgctggacg
     6301 acgctcagag agaactggag cagaagatca agctatagac catacgccgc aggtcatggg
     6361 cttgggcgag aggcccgggc atcacgccga agacgacagg ctccgccgat tcgcagggta
     6421 aggcggacgg cggagcctgc tttggcggaa tgcagttcca ggaatcaaca cggatcacga
     6481 ggtgtacggc atgccgcatc atgatgagtt cagagtgtgc gccgagtatg cgtgcggcag
     6541 cggcgccagg ttgcgcctca ctgacggctt cgtaacggcg cagttcgaca acgttgggca
     6601 gctcgtgccg cgagtcgagt tcttcttccg cgatgggcgt ctacacataa ctcattcatt
     6661 ggcgacagaa cccatgaacg taggagacct tgagtcgcgc gcatgcatag tggatccgga
     6721 taccggcgcc aaactggagg tggaaagcaa cccatttcgg ttcactttgg tatctgccga
     6781 tggacggacg ctcttgcaca atgcgtccgg cgaaccgcat ttcaagtttc gcgctgctcc
     6841 cgatggggag atgttccagt ttacgctgag cctgggtagc tccgcatccc actattacgg
     6901 cttcggcgaa cgatttgact cgctggacca gaatggactc aaccctgacg tatgtgtagt
     6961 gaatcagtac tgcagacagg gcagtaggac gtatatccct atgccatggt ttgtgaccga
     7021 ggtgggctat gggatacatg tcgttactga taggtatgtc cggttcggtc tggctccgcg
     7081 gttgccggga acgatggtca ttgaggccca gatcgatccg ggcaggcctg ttctggagtc
     7141 agcggtcttt ctggggactc ccgctgacat cgtccgcgct tatgccaggt ctactggcgc
     7201 gacggcgctt ccacccaagt gggcgtttgg cccgtggatg tcgggaaacg cgtggaatac
     7261 tcagcgtgac gtggaggagc agctgcgggt cagtgatgag cttggcttgc ctgcaacggt
     7321 cgtggtcatc gaggcttggt cagacgaagc cacgttctac atatggaacg acgctcatca
     7381 cgcgcccgaa tcgggcgaat atggattcag gctcgatgag ttcacgtttc ctgctgaggg
     7441 caaatggcct gatcccaagg cccttgcaga ccacatacac gatagcggga tgaaactggt
     7501 attatggcag attccggttc tgaaacgatt ggctgcgcac gaacccgggt gcgcgcaaca
     7561 tgagcgtgac gaggagcatg ccatcgcccg aggttatgtg gtccgaaacg ctgacggaac
     7621 accataccga atacccgatg ggtggtttgc ggggagcctg ctcttggact tcacaaaccc
     7681 ggaggcgacc gagtggtggt tcagcaagcg gcggtacctc atagaccaac tcggagtgga
     7741 tggcttcaaa accgatggcg gggagttcat cttcgacgac agagtgtcat tctgggatgg
     7801 gcgcaacggc gcttccatgc gcaacaggta tgcgatgact tacatcgagg cataccgcga
     7861 tttcgccgga cccggtcgga ttaccttcag ccgggcggga cacgccggcg ctcagtcgtc
     7921 gggcctgttc tgggcgggcg atcaggaatc gtcgtttgcg gagtctcgtg cggttctgac
     7981 tgccgggctc agcatcggcc tgtcaggcgt tccgttttgg gggttcgaca tggccgggct
     8041 atccggcgag atccccacag ctgagctgtt catccgggcc ctcgagatgg ctgcgttctc
     8101 gccggtgatg cagtaccata gcgatggccc agcagcatgg gaccggacac cctggaacat
     8161 cgcggcgcgg accggagacg agcgcgtagt gtccacttac cgaacttacg cgaacctaag
     8221 gatgaaccta ttgccctaca tctacaacga ggcagtgagc tcggccaagt cgagcgagcc
     8281 tctcatgagg gcactgatga ttgacttccc ttgcgatccg gcgtccttct caattgacga
     8341 tgagtacatg ttcgggcgcg acctacttgt ggcgcccgtg ctgcacgaac atgctcgcga
     8401 gcggtcggtt catctgccgc ctgggcagtg gctggatttc tggaccttcg aggagattgg
     8461 aacgggacca tgcacgatgc cgagctaccc cagcgatatt gggaaaatcc cggtgttcgt
     8521 cagggcagga gcgatcctcc ctatcaacct aggtgatagc atgcaccttg gcagcacaac
     8581 gggggttcgc ggcgtcggat accggaacct ttcgttcctc atcaccggcg tgccggctca
     8641 ggaatgggta ttcactgacg atgaaggcac aaggatccgg ttcgttccgg gcgacggcgg
     8701 attgcgcgtc gttgcggact atgccggcgc cgtggaccat gtgaacttgc tcatcctggg
     8761 gcatggcggg cacggccagc gtgagcgtgc cgaccgccaa tgtagaactg tctgggccgc
     8821 gggaggccgg agggcagagg tggttcgggt cgggactgaa agcttgatac aagggatcct
     8881 acttccgctc ttactgccgt aacgtgcaac ctgcccactg cgcttaccgc cggggctcag
     8941 gccaagctga gcccggcggt tcttccttgc tccacagcgt cggaccggca gaacctgtag
     9001 gtgcgtagac cagacctagc gaacgctcac ctacaagaca tccgcctatt gggcccatac
     9061 actcaaggaa caatccacat acgcggcgaa ttccctgtcc agcggagtgt gaaggggatg
     9121 cgatgaggag acgatggaga aacgatgagt agactagaga gaaccaacgg tcgcgaaagg
     9181 agcacccaga tgtcaaccga catgatgtac ccgaaccctg aggccgatgc ccgggccgag
     9241 gctcagggat tcgatgcgcg caggcttagg catgctgtgg accttgtgaa gaggcgatgc
     9301 gccggcgaaa gcgatcatcc atcataccct ggggctgtgt tactgataat gcgccatggt
     9361 gtcgaggtag tgcatgaagc attcggcttt cgatcatacg atgaatcggc tgagcccatg
     9421 acgcgcgata ccatattcga cgtagcatct atgagcaagc ccatagccac ggcaactgct
     9481 gtcctgagac ttgtggagac gggcgaggtt aacctagacg acccgatcgc gcggtacata
     9541 cccggggttg gcatcgctgc agatggagct ggtgcgggcg ccggcgccgg cgccggagct
     9601 ggagccgaca ttacgctcat gaacctgctc acccatacgt caggcttgcc gccatcgact
     9661 acggtattca agggtgcaga gagcagggac gactgtgtgc gcattgcgcg ttcggtattg
     9721 ccggagtatg cggtaggaag caaggttgag tacagctgca tgggtttcat cttgctcgca
     9781 ttgcttgtcg aggcggtcgc cggggagagc ctcaatgtgt ttgcccgaag ggaggtcttc
     9841 gacaagcttg ggatgaagga tactggctac tatacagtag agcgattcgc cgaacgggcg
     9901 cgggtggcgc ccactgaaag caggacctcg tgcgaaaagg gcgaggaact atgggcgtgg
     9961 ctaaacaaga cagggcagtt cctggacctt cacacccggc ggcaagtggc gtggggcgcc
    10021 gtacacgatg agaatgcgct tgccatgggc ggggtgagcg gcaacgcggg agtcttctcc
    10081 acagcggccg acattgctag gttcgggcag atgtacctac aacacgggtg ctacatgggg
    10141 gcaagggtgc tgtcgcctgc aaccgtggaa cttgccacgc gcaacttcac cgccggcatg
    10201 ggcgacaacc ggggcttggg gtggcagctc aagggcctca acacgtcatt cgggagcctg
    10261 atatccgaca gagcatatgg ccacaccggg ttcaccggca cagtcctctg gatcgaccca
    10321 gatcgtgaac taattgtcgt gctccttacg aacagagtgc acgagacgag agagaatcag
    10381 gcgttggtgg atcttcgacc gaggtttctc aatgcggttg tggctgcgat aagcagatag
    10441 aagagccggc gtggtgttct ggcttggcgg gacctaacga ggttcagagg catcctgtcc
    10501 gtttttggac gggattttcg agcatcctgt tcagatcctg cctggtttta cacgcgataa
    10561 ttgtctgaat tcgcgccagc acgggtgctg gcttcctctg tcatcatgcc gccgctgaat
    10621 ccgcaggcag aggcgtttgg tataatctgg ccacgcaact ctatgccctg gatatgcccg
    10681 aggcggagct agacggtcgg aaatccccca tgaaacgcca aaaggtccca agattgacct
    10741 gcccccggtg aaaaccgggc acttcctgag caggtcgtcc gaaaaaccgg gcacattcct
    10801 cacaggttcg cgaactccct gctaaacacg ggtttgccta aaggcctgtg ccacatcgcc
    10861 ttcaccaaca aatcgcatct ccgagcgtgg gcacattccc cacgaaaaat ctttgcgagc
    10921 ggcaagatat ggcggaagga tattatgatc atagcgcgaa atggtattac gtaatacctt
    10981 tcgcgctaca gtgaaagggt ggtctgttgt gaaaacccta gagccaattg gagacgggca
    11041 gtcggtgcca tcgcgcgcat acgagcggat aatggagttc attgccgagg gcgaccttgt
    11101 cggcggcaaa cttccccccg agacagtcct agcacagcag cttggggtga gtcgcacggc
    11161 tcttcgggag gcgctacagc gtctcgaatc cgaagggtac attgtgaggc ggcgcagggt
    11221 gggcactatt gtgctcgcaa gccggctcaa attggacgca gggcttgaga ggctgaatt
//
LOCUS       DAOTAC010000050        11016 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_5066_length_11016_cov_4.803029, whole genome shotgun sequence.
ACCESSION   DAOTAC010000050 DAOTAC010000000
VERSION     DAOTAC010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 11016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11016
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5066_length_11016_cov_4.803029"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..1019
                     /locus_tag="PLM74_03505"
     CDS             156..1019
                     /locus_tag="PLM74_03505"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HON42183.1"
                     /translation="MSTAGRAASRAACRAARSAAGGAVRTPARTAGFVWEHFKFNLAS
                     AMEYKWSFVSQVSFMVLNDFLLLFFWWLIFGKVQTIGGWTFADVMTMHAVLATAYGLS
                     VSVFGNVNRLARIIAEGQLDYYLLLPRNPLVHVLISRSDISGLGDVVFGLVAFCLFTP
                     ATPGRIALFVIVSLASCTVYTCFAVITGSIAFFIGNAQTISAQLVGALITFAGYPGSI
                     FKGLVRAVLYSVIPVGFVIYVPVSLLRSFSLSHFAGLLAFAAAMVCGASWLFAAGLRR
                     YESGNLVSARI"
     gene            complement(1016..2296)
                     /locus_tag="PLM74_03510"
     CDS             complement(1016..2296)
                     /locus_tag="PLM74_03510"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HON42184.1"
                     /translation="MPTKRLYYDDPYLAGFTCQVLHCEPAARNSHSAPLYEVITDLTA
                     FYPNSGGQPSDRGTLAGTDVVDVIERDDEVVHITSTAVPLGPAQGRIDIERRFDHIQQ
                     HSGQHILSGAFASLFGMETVGFHLGDEYVTIDLNSPDISHDQWAAAERLACEVVFQNR
                     PVRATFWDRDALNDAIDPSRLRKLPTRTENIRLVDINGFDLTPCGGTHVRASGEVGLI
                     KITRTERIRGITRVEFVCGRRALADYRWKNQAAYEAAAMLSVHARDLPESVSRLQMQL
                     RDTARQLEHALKDLYRYQAEAMYDSAEPAGESGIRVVSWASTDGHDIGYLKMVAHEIC
                     QHPSTVALLGSSNGESAHVVFEKSHDVSIDMAKLLREVLPSVDGKGGGTHQIAQGGGR
                     RPDRLPYAISLAAEMVSKAVAGIEAETAASADRH"
     gene            2397..3029
                     /locus_tag="PLM74_03515"
     CDS             2397..3029
                     /locus_tag="PLM74_03515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HON42185.1"
                     /translation="MTLGYLTVRGRGRAEIVVKRSKFIATASPVAAEEDAQGFVDEVR
                     REFWDATHNVYAFVVGEHNEVARSSDDGEPSGTAGRPVLEVIRREDVHYCAVVVTRYF
                     GGTLLGAGGLIRAYSAAAREGLYAAGIARAQPMCEVRFVIEYPMLGKVQNQLAETGVE
                     TVDVDYAERVTMRVMVPSDALDLFAKTMTELLSGAFEPQVGEERLALVDP"
     gene            complement(3111..6644)
                     /locus_tag="PLM74_03520"
     CDS             complement(3111..6644)
                     /locus_tag="PLM74_03520"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HON42186.1"
                     /translation="MSCAAKPRANGRCRGCSTEHMKDFVHLHVHSQYSFLDGADTCTT
                     LVARAASFGMPAIAITDHNNVSAMVELTKAAAKHGIKPICGTELTVRHEGSETHEDRS
                     NGGDTARAARTDRPAGHPLSHLTLLAADPCGYSNICRILTDAYMSSPRLDPHASLEIL
                     RRHSGHIIALSGCRRGRVASLIAAGRFVEALAAAKELASIFGHDNFYIELQHTLTPGA
                     SALNASLAELASAAGLGLVATNNVHYAEKSRFEVHDALTCVRTLTTLDDIHPERRVNA
                     ENYLKSSEEMGELFAKYPEAIANTLAIAERCTPGLDLSRRLFPKYQASPGGRGCGNGS
                     VSANDAGNSNDSARHPAGEPAAAILRRLTMQGAVERYGRITPAIRSRLDHELNIITTL
                     GFEDYFLAVWDIACWARSQGIRYAGRGSAADSAVAYCLRLTNVDSIARGLLFERFMSL
                     ERAQKPDIDIDFESERRDDVARYVYQRYGEEHVASVCTFNTFQGRSAVRDFGRVLGFP
                     QADIDRLAKTLPHVPADAVRAAFSRYPEMRDSGIPAWKYELLLTLAESVAGYPRHIGT
                     HLGGLVISSEPLTCVTPLQMAAKGIAITQFDKDTIEDLGLIKLDLLSLRTLGAVEHAV
                     DMVRGSGATSDNRATSGSGATNGSHADARSDSCAARSDGSGSGPDPGFDFDRIPHGDR
                     ATYEMLNAGETIGVFQLESPAQRALQTRLGADSFEDIVASVALIRPGPIQGNMVEPFI
                     ARRRGDEEISYIDPRLAPILDKTYGVVLYQEQVIQIATTIAGFTPGEADRLRKVMTHF
                     RSMREMEEIGRDFVAKAMAGGTDPKVAETIFSYIVGYAGYGFCEAHAAAFADTAYRTA
                     YMVRHYPAHFYAAVLSAQPMGFYSPRTLLVEAKRRGVRVLPLDINRSQEKYSVETIEG
                     TGGKTKDEAIGPAIRVGLMQVKGISKDAIRRILAAREEGKPFRSAPDFLYRVEIPRDI
                     VQNLALAGAFDSLEPNRRALAWRLGSLADDAAALRAACAAAHGSGQAALVGTLEPPIA
                     YETHVGSEMPDFTEVEKFKHEMSILGLCVSRHAMEFFRPVLARGGVLTSREIAGARHG
                     QAVRTAGLVVRPHRPPTRSGKTVVFLSLEDEMGLTDVTVFESIYQKYGSLIYGSPSLL
                     ISGTVSRRGDGVSVIARSIQRLGRRQGSSAK"
     gene            complement(6589..6834)
                     /locus_tag="PLM74_03525"
     CDS             complement(6589..6834)
                     /locus_tag="PLM74_03525"
                     /inference="COORDINATES: protein motif:HMM:NF039800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6504 family protein"
                     /protein_id="HON42187.1"
                     /translation="MARKIDAHVRVVEGPAGSPSRFYWPDKWRPVAQVIDYWRESGEW
                     WDGEEEKDYYLITSAGGGCYELCREAPGEWALSRVLD"
     gene            complement(7649..9025)
                     /locus_tag="PLM74_03530"
     CDS             complement(7649..9025)
                     /locus_tag="PLM74_03530"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42188.1"
                     /translation="MRSIAYIHIPFLHSAAHVMLNPDLRGKSFAAVNKNTVIGVSPEL
                     SAAGVLPGATRRHARQACPGAVFVDQEPDAYPRLAGKVWDICYEHTPLVEPIAIDGAF
                     TDFTGCGDARAIADSIVSESRDKARLEVRIGLAGNRLMARVAVEADVGMGVSVGIGVG
                     AGRAAGAGADAGPDASVAAGVAVGPHPEQVITVASGTEERLLSKLPVGSLWPLSPDTV
                     DKLIKLGINTVGQLQAVPLPMLTSQLGEAGLAAHLLSRGIDRSPVRAAYPEETIECYM
                     SFDFPVCEWPDLEQCLRVCTSEIAARLSASGSRAVSCIELELETAQRASAAGGIAPKG
                     AGNSNGKRDSADNIDGRSSGPVLKRHYFRRPADSLQQITRAVIHAAEGRVSGPVSAIR
                     VRASGLVAPAAVQLSIFGPAAQPETGQRELEHAIESIREKFGARSVTTANTLVRSRRD
                     RMLAESLI"
     gene            9271..9732
                     /locus_tag="PLM74_03535"
     CDS             9271..9732
                     /locus_tag="PLM74_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42189.1"
                     /translation="MHCDALYTGKSAAPVVGDLSWHFDTNPDPSVDLRLANFEESLEA
                     AVFLLERGWEKAAFYVLGYGIHALQDVFAHGDSTPLDHVWAVVIGTMQKEGQTGKLEL
                     WSWMDAADMDGPNTPAQGGESQRAQTAKEVTTAILQGFRDLWQSIRGLFRD"
     gene            9774..10517
                     /locus_tag="PLM74_03540"
     CDS             9774..10517
                     /locus_tag="PLM74_03540"
                     /inference="COORDINATES: protein motif:HMM:NF012632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42190.1"
                     /translation="MNTTAHPDRKAVLKLAATALCIVTLASTWSAIGSYSAETAPGPG
                     STIAAGSGYSLLIGAGGSVWGWGGNDSHQVNGDMWQISYAPRRVGISGVVSVAAGSSF
                     SMALTGDGLVWQWGEISEKFGKSVYLRIPTRVLGLPRARAISAGGDHSMAMCSDETVW
                     TWGQNGHGQLGGGDAKYRATPGIVEGLSNVVAIDAGSGSCISLKADGTVWTWGYRSEF
                     DSRGMGSCTGFGGRRYCVGAGRQEAALSV"
BASE COUNT         2064 a         3152 c         3495 g         2305 t
ORIGIN      
        1 gactcctttc aactatgtgc tgtatgcgcc ggcgcgcctg ttcgtccagt tcgacatgga
       61 cttcttcctg agcaccgtga cgatgcaggc agtgtgggtg gctgtcctgg cgctactggt
      121 cacggcgctg tacggcatgg gggtgagacg gctcaatgtc aacggcgggt agagcagcga
      181 gtagagcagc atgcagagca gcgcgtagcg cggcgggcgg ggcggtgcgc acacctgcgc
      241 ggactgcagg tttcgtgtgg gagcacttca agttcaacct ggcctcggcc atggagtaca
      301 agtggagttt tgtctcccag gtgtcgttca tggtgttgaa cgacttcctg ctcctgttct
      361 tctggtggct gattttcggg aaggtacaga ccatcggcgg ctggaccttt gccgatgtca
      421 tgacgatgca cgcggtgctg gcgacggcat acgggttgag cgtgtcggtg ttcggcaatg
      481 tgaatcgtct ggcccgcatc atagccgaag gtcagctcga ttactacctg ttgctgccac
      541 gaaacccgct agtccatgtg ctcataagtc ggtcggacat ttcgggcctt ggcgacgtgg
      601 tgtttggcct cgtggcgttc tgcctgttca ccccggccac ccccggcagg atagcgctgt
      661 ttgtcatcgt atcgctggcc tcatgcactg tgtacacttg tttcgcagtc atcacgggca
      721 gtatcgcgtt ctttatagga aacgcccaga ccatcagcgc acagctcgtc ggggccttga
      781 tcacattcgc tgggtacccc ggctcaatct tcaaaggcct ggtccgggcg gtgttgtact
      841 cggtaatacc tgtgggtttc gtaatctacg tacccgtttc gttgcttcgc agcttcagtc
      901 tctcccactt cgcaggtttg ctggccttcg cggctgcgat ggtatgcggc gcatcgtggc
      961 tgtttgcggc cggcctccgt cgctacgagt cgggcaacct cgtatcggcg aggatctagt
     1021 ggcggtcggc cgacgcggcc gtctccgctt cgatgccggc tacagctttc gataccatct
     1081 cggcggccag ggagatcgca taaggcaacc tgtcggggcg cctgccgcct ccttgggcta
     1141 tctggtgagt accgcctccc ttgccatcga ctgaaggcaa cacttcgcgg aggagttttg
     1201 ccatgtcgat agacacatca tgggattttt cgaacacgac gtgcgcggac tcaccatttg
     1261 acgagcccag gagggctacg gtactcgggt gctggcagat ctcgtgcgcg accattttga
     1321 ggtagccgat gtcgtgccca tcggtagatg cccatgacac tacacggata ccggactccc
     1381 cagccggctc ggcgctatcg tacatcgcct cagcttgata ccggtacagg tccttcaggg
     1441 catgttccag ctgacgcgcg gtgtcgcgta gctgcatttg cagacggctt accgattcag
     1501 gcagatcgcg cgcgtgcact gagagcatgg cggctgcctc atacgccgcc tggttcttcc
     1561 agcggtagtc tgcgagcgcg cgacgcccgc agacgaactc cacacgggtg atgccccgga
     1621 tacgttcggt gcgggtgatt ttgatcaggc cgacctcgcc ggaggcccga acgtgcgtgc
     1681 cgccgcaggg ggtaaggtcg aaaccgttga tatcaacgag gcggatgttc tcggtcctgg
     1741 ttggcagctt gcgcaggcgc gatgggtcga tggcgtcatt cagggcgtcg cggtcccaga
     1801 aagtggccct aacgggccta ttctggaaca cgacctcgca cgcaaggcgt tcggctgcag
     1861 cccactggtc gtggctgata tcgggagaat tgaggtctat cgtaacgtat tcgtcaccta
     1921 ggtggaaacc gacggtttcc attccgaata ggctcgcgaa tgcaccggac aggatatgct
     1981 gcccgctgtg ctgctggatg tggtcgaacc tgcgctcgat atcaattctg ccctgtgcag
     2041 ggccgagcgg gacagctgtg gaagtaatat ggacgacttc atcgtccctt tcgatcacgt
     2101 cgaccacatc cgtcccggcc agagtgccgc gatcggacgg ctggccccct gagttggggt
     2161 agaacgcggt taggtcagtg ataacctcat atagaggtgc tgaatgggag ttcctggcgg
     2221 caggctcgca gtggagaacc tggcaagtga agcccgcgag gtagggatcg tcgtaataca
     2281 gcctcttggt tggcactgtt cgaattcgcc tcctcaagct cttttcgagc cgcggttgcg
     2341 cttaggtact tctactgcgg aacaggattc cctctcacga acaaggggtg gtttttgtga
     2401 ccttgggcta tttgaccgtg cgcggccgag gacgcgcgga gatcgttgtg aaaaggagca
     2461 agttcatcgc tacggcgtca ccagtcgctg ccgaggaaga cgcgcaggga ttcgtggatg
     2521 aggtccgccg ggagttctgg gacgccactc ataatgtata tgcgttcgtc gtcggcgagc
     2581 acaacgaggt agctcgctcc agcgacgacg gcgagccttc cggcaccgcc ggccggcccg
     2641 tgctagaagt catccgccgc gaagatgtgc attactgcgc agttgtggtc actcgctact
     2701 tcggcggaac actgctcggc gccggcggac tgatcagggc ctacagcgcg gcggcgcgcg
     2761 aagggctgta tgctgcgggc atcgcgcggg cgcagcccat gtgcgaggtg cgctttgtca
     2821 tagaataccc aatgctgggc aaggtccaga accagctagc cgagacgggc gtcgaaacgg
     2881 tcgatgtcga ctacgccgaa cgcgtgacca tgcgggtcat ggttccatcg gatgcccttg
     2941 acttgtttgc gaagaccatg accgagctgc tatccggggc tttcgagccg caggtgggcg
     3001 aggaacgcct tgcgctggtc gatccatgac ccggcgctac tacgcggcgc catgacgcgg
     3061 cgccatagcc ccgcgccata gccccgcgaa ccgtggcgct gcggacgccg ctattttgcc
     3121 gaactgccct gccttcgccc cagcctctgt atggaacgcg caattaccga cacgccgtcg
     3181 ccgcgccgcg acaccgtccc tgagatgagc agggacgggc ttccgtatat cagtgaccca
     3241 tatttctgat atatgctttc gaacaccgtc acgtccgtaa ggcccatttc gtcttctaaa
     3301 gacagaaaca ctaccgtctt gccgcttcta gtggggggcc tgtgggggcg caccacaaga
     3361 cctgctgtgc gcaccgcctg cccgtggcgc gcgcccgcga tttcgcggga tgtgaggacg
     3421 ccgcctcgtg ccaggaccgg ccggaagaac tccatggcgt ggcgcgacac gcagagaccg
     3481 aggatggaca tctcatgctt gaacttctcg acttcggtga agtcgggcat ttcggacccc
     3541 acatgtgttt catacgctat gggcggttct aaggttccca caagtgctgc ctgccccgat
     3601 ccgtgggcgg ccgcacaggc ggcgcgaagt gcggcggcat cgtcggccag actgccgagg
     3661 cgccaggcga gcgcccggcg gttgggttcc aatgaatcga aggccccggc cagcgcgaga
     3721 ttttggacga tgtcgcgcgg gatctcaact cgatagagga agtccggcgc cgaccggaaa
     3781 ggcttgcctt cctcgcgggc cgccaggatc ctgcgaattg cgtctttgga tatgcctttg
     3841 acctgcatga ggcctacacg gatagcagga cctatcgcct cgtctttcgt tttgccccct
     3901 gttccttcta tggtctccac cgaatatttc tcctggctgc ggttgatgtc gagcggcagc
     3961 acacgcacgc cgcggcgctt ggcctccacg agcagtgtgc ggggcgagta gaaccccatg
     4021 ggctgggcgc tcaggaccgc agcgtagaag tgggccgggt agtggcgcac catgtatgcc
     4081 gtgcggtagg cggtgtcggc gaacgcggcg gcatgcgcct cgcagaaccc atagcccgcg
     4141 tagcccacaa tgtaggagaa gatggtttca gctaccttcg gatctgttcc gcctgccatg
     4201 gctttggcca cgaagtcgcg gccgatctcc tccatttcgc gcatggaccg aaagtgtgtc
     4261 atcactttgc gcaggcggtc ggcttcgccc ggggtgaaac ccgctatggt cgtggcgatc
     4321 tggatcacct gctcctggta gaggacgact ccgtaagtct tgtcgagaat cggggccagc
     4381 ctgggatcta tgtaggagat ctcctcatcg ccgcggcgac gcgcgataaa cggctccacc
     4441 atgttgccct gaatcggccc cggtcggatc agagcaacac ttgctacgat gtcttcgaag
     4501 ctgtcggcgc cgaggcgtgt ctgcagggcg cgctgggccg ggctttccag ctggaacacg
     4561 ccgatggttt cacctgcgtt gagcatctcg tacgtggccc tgtcgccgtg gggtatgcgg
     4621 tcgaagtcga agccggggtc ggggccagag ccggatccat cactgcgagc ggcgcaggag
     4681 tcgctgcgag cgtcggcgtg actgccgttg gttgcgccac tgccgctagt cgcacggttg
     4741 tcgctggtcg caccactgcc gcgaaccatg tctacggcgt gttccactgc ccccagtgtt
     4801 cgcagggaga gcaggtccag cttgatgagg cccaggtctt cgatggtgtc tttatcgaac
     4861 tgggtgatgg cgatgccctt ggcggccatc tgaagtgggg ttacacaggt aagaggttcg
     4921 cttgagatga ccagcccgcc caggtgagta ccgatgtggc ggggatagcc tgccacagac
     4981 tctgccagag tgagcaggag ttcatatttc cacgcgggta tgccggagtc gcgcatctcg
     5041 gggtaccggc tgaaagctgc gcgcactgcg tccgccggga cgtggggtag tgttttggcc
     5101 aggcgatcga tatcggcctg cgggaatccg agtacccggc caaagtcgcg cacggccgac
     5161 ctgccttgga aagtgttgaa tgtgcagact gacgcgacat gctcctcgcc atagcgctga
     5221 tatacgtagc gtgccacatc atcacgccgc tctgactcga agtcgatgtc gatgtcaggc
     5281 ttttgggcgc gctccaggct catgaatctt tcaaagagca atcctcgggc tatcgaatcc
     5341 acattggtca ggcggaggca atacgccacg gcggaatcgg ccgcggatcc gcgaccggca
     5401 tagcggatgc cctgcgaccg ggcccagcat gcaatgtccc aaacggcgag gaagtagtct
     5461 tcgaagccca gggtggttat gatgttgagc tcgtggtcca gcctcgatcg aatggctgga
     5521 gtgattcgac cgtaccgttc gacggcgcct tgcatcgtaa ggcgtctgag gatggcggcg
     5581 gccggttcgc cggccggatg cctggcggag tcgttggaat tgccggcgtc gttggcggaa
     5641 acgctgccgt tgccgcagcc ccggccgcct ggcgaggcct gatactttgg gaacagacgg
     5701 cgcgatagat ccagccctgg cgtgcatctt tcggctatcg cgagagtgtt ggcaatggcc
     5761 tcggggtatt tggcgaacaa ctcgcccatt tcctctgagg atttcaggta gttctcggcg
     5821 ttcacgcgcc gctcggggtg tatatcgtca agggtggtga gggttcggac gcatgttaac
     5881 gcgtcgtgca cctcgaaccg ggacttctct gcatagtgca cgttgttggt ggcgaccagg
     5941 ccgaggccag cggcggatgc cagctcggcc agggaggcgt tgagcgccga cgcgcccggc
     6001 gtcagtgtgt gctgcaactc gatgtagaag ttgtcatggc cgaatatgga ggcaagttcc
     6061 ttggctgccg ccagagcttc gacgaacctt ccggcggcaa tcagggaggc aacgcgtccg
     6121 cggcggcagc ccgacagtgc gattatgtgg ccggaatgtc gtctcaatat ctccagggag
     6181 gcatggggat caaggcgggg tgaggacatg taggcatcag tcaatatgcg gcagatattg
     6241 gaatagccgc aggggtcggc agcgagcagg gtaaggtggg agaggggatg gccggcaggg
     6301 cgatcggtgc gggcggcgcg ggcagtgtcg cctccgttgc tgcggtcttc gtgagtctcg
     6361 gatccctcgt gccttactgt cagctctgta ccgcatattg gcttgattcc atgctttgcg
     6421 gcggctttcg tcagctctac catggcactg acgttgttgt ggtcggttat ggctatggcg
     6481 ggcatgccga aggatgcggc ccgggccacc agagttgtgc aggtatccgc gccgtcgagg
     6541 aaggaatact gggaatggac gtgaagatgg acgaagtctt tcatgtgctc agtcgagcac
     6601 ccgcgacagc gcccattcgc ccggggcttc gcggcacagc tcatagcagc cccctcccgc
     6661 cgaggtgatc aggtagtagt ccttttcctc ctcgccgtcc caccattcac ccgactcgcg
     6721 ccagtaatcg atgacctgtg cgaccgggcg ccatttgtcc ggccagtaga accgggacgg
     6781 cgaaccggcg ggcccttcta caacacgaac gtgagcatcg attttcctgg ccatagaaat
     6841 cacgtcctat ctgctagact gaaagcgcgc tcttgtcagc ccatgtgccg acatcacctg
     6901 tattcggatc tggcctccgg catgcaatcc cgtcaggagc acaacgaaga cagccggagc
     6961 tgaatgggac ccggggatag acccaggggg cagatccgag gacaagtcca cagatagatc
     7021 caagggcaga cccgagggtc gacccaagga ctgcccgaag gatgaaaggg caattaatgt
     7081 agtccggtgt tatacccccg ggggtatcca agcagagatg ccgtcagatt ttgataacag
     7141 ttttgccgat ggacgcagag tttgagtaca tgcatttgag tccaggccgc ctgggtatca
     7201 tagacagcta taaagggatt ttacagacag atcttctgat attataagtc aagaacatgg
     7261 cataacgcac cgtctctcgc gaggcaatac caaatatatc cgtataatgg gatatcatgt
     7321 ttgccaggct gtccgatttc gcgcccggac tgataggata ccccccgttt tcgtggtttg
     7381 gaggcagttg ggtgctcctc cgaaatcgat gtactcaaaa gttgcgggca tctccgaaac
     7441 tattgatgaa aaatgacact atcgcagctc aggccgtttt gacccacagt tatcgcagct
     7501 agaccagcta aagggccttt tccgccagga tgcccagcga ccccgaaaat ccaggaccac
     7561 tcgaacccag aaactccggg ggtctcacta gtggtcatcc tcgaacaaga ttattgctgc
     7621 catattatta cctatataac ggaggaaact agatcaacga ttcagccagc atgcggtcgc
     7681 gtcgcgacct tacgagagta tttgcggtgg ttacgcttct tgcgccgaat ttctcccgta
     7741 tagactctat cgcgtgctcg agttcacgct gcccggtttc gggttgcgct gccgggccga
     7801 atatggacag ctgtaccgct gcgggcgcta ctagcccgga agcgcggacg cgtattgccg
     7861 acaccggccc ggacaccctg ccctcggccg catgtatcac agctcgcgtg atttgctgca
     7921 aggagtcagc ggggcggcga aagtaatggc gcttcagtac cgggccgctg ctcctgccat
     7981 cgatattgtc ggcactatcc cttttaccgt tgctgttccc ggcgcccttt ggtgctatgc
     8041 cgccggcagc gctcgcccgc tgcgccgttt ctagctcgag ttcgatgcag gatacagcgc
     8101 gactgcccga ggcgctgagc ctagcggcga tctcagaagt gcatacgcgc aggcactgct
     8161 ccaggtcggg ccactcgcat acgggaaagt cgaacgacat gtagcattca atcgtttcct
     8221 ctgggtacgc cgcacgaaca ggggatcggt cgatgccccg gctcaacaag tgagccgcaa
     8281 ggcccgcttc gcccagctgg gaagtgagca tgggcaaggg aaccgcttgg agttggccca
     8341 ctgtgttgat ccccagcttt atgagcttgt cgacagtatc cggcgatagg ggccagagcg
     8401 aaccaaccgg taacttcgac agaagccgct cttccgtgcc ggacgcgact gtgatcactt
     8461 gctctggatg gggacctaca gctacgccgg cagcgacgct ggcatcggga ccggcgtcgg
     8521 cgccagcacc ggcggcgcga ccggcaccaa cgccgatgcc gacgctgacg cccatcccca
     8581 catccgcttc tactgcgact ctggccatga gccgattgcc cgccagacct attcgaacct
     8641 ctaagcgagc cttgtccctg gattcgctca cgatggaatc ggctattgct cgggcgtcgc
     8701 cgcaaccggt gaagtcggtg aatgcgccgt ctatagcgat gggctcaaca agcggagtat
     8761 gttcgtagca gatgtcccac accttgccgg cgagacgcgg gtaggcatcg ggttcttgat
     8821 ctacgaagac cgcgcctggg catgcttgcc gagcgtgcct gcgcgttgcc cctgggagca
     8881 cccctgctgc ggacaattcg ggcgatactc ctatgacggt gtttttgttg accgccgcga
     8941 aggatttgcc ccgaagatcg ggattgagca tcacgtgagc ggcggaatgt aagaatggga
     9001 tgtgtatgta agcgatggag cgcattctga tacgcctccg ctatagaaca tatgttcggt
     9061 aaactcatta tatgcggaga acggcgggaa gtcaacgaac ctgaagctga acctgaagct
     9121 gaacatctta cttgttcagc gcggtcagca gtcccgcctg ccgtaagttc ggatttcgcc
     9181 gaataacggc accaaatgca caagacccgg aaagcagcag ctgccgttgg cttcagtgat
     9241 ggtctggcta atgccctgaa gctgggagcc gtgcactgtg atgctttgta tacggggaag
     9301 tctgcggcgc cagtcgtcgg ggatctgtcg tggcatttcg ataccaaccc cgacccgtcc
     9361 gtcgatttgc gtctcgcgaa tttcgaggag tcgcttgagg cggcggtctt ccttctcgaa
     9421 cggggatggg agaaggcagc gttctacgta ctgggctacg ggattcatgc tcttcaggac
     9481 gttttcgctc acggcgattc gaccccgcta gaccatgtat gggccgtcgt aattgggacg
     9541 atgcagaaag agggacagac aggcaagctc gaactgtgga gctggatgga cgcagcagac
     9601 atggacggcc ccaatactcc ggcccaaggc ggagaatccc agcgggctca gaccgctaag
     9661 gaagtgacga cagccatact gcaggggttt cgtgatctgt ggcagtcaat ccgcggactg
     9721 tttcgggact agcggcggcc tatggacaca cgtcagggaa gggggttgcg cgggtgaaca
     9781 cgaccgctca tccggatcgg aaggccgttc tgaagctggc ggcaaccgcg ctctgcatcg
     9841 tgaccctcgc gtcgacatgg agcgcgatcg gaagttactc cgcagagact gcgccgggac
     9901 ccggttcgac cattgctgca gggtccggat attcgctcct catcggcgcc ggcggaagcg
     9961 tgtggggctg gggcgggaat gactcacatc aggtgaatgg cgacatgtgg cagatttcat
    10021 atgcgccacg cagggtcggc atctcgggag ttgtctctgt cgcggcaggc agtagcttct
    10081 cgatggccct gaccggcgat ggcttggtct ggcagtgggg cgagatatcc gagaagttcg
    10141 gcaagtccgt ctatcttcgc attcctacgc gcgtccttgg cctgccgcgg gcgcgggcga
    10201 tatccgctgg tggggatcac agcatggcga tgtgcagcga cgaaactgta tggacctggg
    10261 gccagaacgg gcatgggcaa ctcggcggcg gcgatgctaa gtatcgtgca actccaggca
    10321 ttgtcgaggg cctgtcgaac gttgtggcca ttgacgccgg ctcgggttcc tgcatttcgc
    10381 tcaaggccga tgggacggtt tggacgtggg gttaccgtag tgaattcgac agccgcggga
    10441 tgggatcatg cactggcttt gggggccgac ggtactgtgt gggagctggg cgccaagagg
    10501 ctgctctttc cgtttgacgt aacctacaat ctactatggc ccgctcgagt agacaccata
    10561 gaaagcggag tccgcttact cccgtaattg cgtatcctca ttgaccgcag ggcgccggcc
    10621 cacactacca ggtggcaccg gcgccttggc ggcagcggca acctgccgaa aagacgcatc
    10681 gagttgccta taggcgcgca tctcgtggag gcatatcctg gatatctata gccaaccaat
    10741 atgggaatga acactttggc ctcgataatg ggcaatgtat atgagccgta ggctgcgtgt
    10801 ttgctgcgaa acgcctatct gcctctacgc attgcccaca tgactggaaa ggatgcaggt
    10861 cttgctgatt ggccgaacac atgccgggct ctgcctacgt tcggagacgc cagttgttgg
    10921 cggtgatttt atgcaggcct ttgggcaaac cctcgtttag aagggagtta gcgaacctgt
    10981 aaggaaaaca gccggttttc tgcgagggct gttcag
//
LOCUS       DAOTAC010000051        10850 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_5176_length_10850_cov_5.433256, whole genome shotgun sequence.
ACCESSION   DAOTAC010000051 DAOTAC010000000
VERSION     DAOTAC010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 10850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10850
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5176_length_10850_cov_5.433256"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(243..692)
                     /locus_tag="PLM74_03545"
     CDS             complement(243..692)
                     /locus_tag="PLM74_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42191.1"
                     /translation="MELTDARGVMLQVTLPGDTGRADVPLRPEEMDAAAHFIRSQGYS
                     PEHFGLEPVGHSNYMAWPVPADQILPEPGGEHEPDAESTLGVSVDLLVEPPKEPSNKP
                     AAGPEAEPAVEPAVEREPPRPKQAAAPDAQAKPHTIVWKAFPRPRSQ"
     gene            complement(754..1896)
                     /locus_tag="PLM74_03550"
     CDS             complement(754..1896)
                     /locus_tag="PLM74_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HON42192.1"
                     /translation="MSEQSTAQVETPARGLGAGISSILRNWSVPIFFLAISIAGIAAA
                     RLDINYLINEVVGRFGRNAFLVISLIIPVMAGLGLNFGIVIGAMAGQTALVIVTHYHI
                     EGLAGFALAAVISVPIAMLLGWLVGRTLNQAKGREMVTSMIMGFFANGIYQLVFLVLL
                     GTLIPMKDPVLMLGSGIGLRNTIDLIGLSYSLDRAMDWLWNGFVFTSIGGTRLGPWRF
                     PICTLFIIGILCVLTTFITRTKLGQEMRAVGQDMHIAQVAGINVDRTRIIAIMMSTVL
                     AAWGQLIFLQNIGTFNTYSSHEQVGMFSVAAILVGGATTRRATIWNAIVGTLLFHTLF
                     VVSPLAGQRLFGVSQVGEYFRSFLAYGVIAVALALHAWQGRAGKSK"
     gene            complement(1902..2966)
                     /locus_tag="PLM74_03555"
     CDS             complement(1902..2966)
                     /locus_tag="PLM74_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HON42193.1"
                     /translation="MSDESKAGIRQLAKNAYEQLGLPRLIIIAFLLSLFALAVIMRMD
                     LSILIKDSITRIGMNGILVLAMLPTLACGVGLNFGLPVGVIAGLVGAVTSMNWGLKGF
                     AGFFAAMAIAIPLAVIAGYLYAMLLERVQGQEMMVGTYVGFSVVSGMCMFWLLAPYRN
                     PAMIFALGGKGLRTTITLDRTFGKVLNDFLGFSIFNVYIPTGLLLFMGVFFVLIALFF
                     KSKAGLAMRTVGSNPMYARSSGINVQRMRTLGVTLSTVLAAIGILVYSQSYGFVQLYT
                     APLTAAFPAVASVLIGGASLRKATIGHALIGTVLFHTLLTIALPVTQTVLQGADISEI
                     ARMIMSNGMIIYALTRAERR"
     gene            complement(2968..4572)
                     /locus_tag="PLM74_03560"
     CDS             complement(2968..4572)
                     /locus_tag="PLM74_03560"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012200076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HON42194.1"
                     /translation="MGSGNVLELKGITKSFFGTTVLKDVNLSMSPGEIHALVGENGAG
                     KSTLMNILFGMQVILDTGGFEGQIVVGGQPVTIDSPRKAMELGIGMVHQEFMLIPGFT
                     VSENIKLNREPTRSNVASRLFGPKLETLDEPLMQSDSRVALGKLALSIDEMALVGGMP
                     VGHMQFVEIAREIDKKNVRLLVFDEPTAVLTESEASLLLAAMKRLSSEGIAILFITHR
                     LDEVMEVADNITILRDGEVVSRMPRDEAKLDRIAQLMVGRKIERPVLPPRKSEPSDKD
                     VVLKIENLKVDMPGERVRELNLAVRRGEILGIGGLAGHGKVGIANGVIGMYPASGLVT
                     KDGVRIPLNDPKHALLSGMAFVSEDRRGVGLLLDESIEMNIILTAMQVQGKFLQPGVL
                     PSLRLEDRAAMREHALSVIRDLDIRCTGPAQLVRRLSGGNQQKVCLGRAFTMEPEVLW
                     VSEPTRGIDVGAKQLVLDLLVKFNREHGMTIVMTSSELGELRKICDRIAIVYRGQIAG
                     VLPPDASDVDFGLMMAGKSATLEEVS"
     gene            complement(4695..5810)
                     /locus_tag="PLM74_03565"
     CDS             complement(4695..5810)
                     /locus_tag="PLM74_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014452182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HON42195.1"
                     /translation="MFKKSLLVIAALILVLGVASVSLGAPAFKIGVVTGTVSQGEDEY
                     RGAEFVVAKYPGMIEHRTYPDNFMAEQETYIAQITSLAADPAIKAIVVNQGVPGTVAA
                     IRKVKQLRPDILFVIGEPHEDPPTIDPVADLSLIPNNPARGRSIPQLAKKLGAKTFLH
                     YSFPRHMSYADLAARRDIMDEECKKLGMQFVFVTAPDPMGEGGLPAAQQFILEDVPRQ
                     VAKYGKDTAFFSTNCGMQEPLITAVLKAGAIFPEQCCPSPTHGYPAALGLEITEQMAG
                     NFPAIVKAINAEIVKRGMSGRMATWPVASSYLNTVAGVEVAKLAVEKKLNIKDMAAVK
                     KVVEQLAGVPMEFERHSATGCYYRYLISSIVFGKDVK"
     gene            6121..7128
                     /gene="glpX"
                     /locus_tag="PLM74_03570"
     CDS             6121..7128
                     /gene="glpX"
                     /locus_tag="PLM74_03570"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HON42196.1"
                     /translation="MSSVEDREREIMLELVRVTETAALRAARCMGMGDCNLVDDAAVD
                     GMRGMLSLININGTIVIGEGEKDQAPMLYCGERVGTGRDECDVDLAVDPVEGTRLVAN
                     GLPNAISVMVVADRGTLMPVPTFYMHKIAVGPEARDYIDINAPVRENLRVVAAALGRK
                     VRDLTVVILNRPRHDQLISEVRECGARIKLIQDGDVAAAISTALPNTGVDMLMGIGGA
                     PEAVLTAAAIKCLGGELQTKLWIRDDEDAARARERGFDDMERVYCSEDLARGNSIVFA
                     ATGITDGDMLQGVRYYGQKATTESIVMRMLTGTVRRIHTTHDLSRKTLRSFKAGQEMI
                     P"
     gene            complement(7100..7975)
                     /locus_tag="PLM74_03575"
     CDS             complement(7100..7975)
                     /locus_tag="PLM74_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42197.1"
                     /translation="MTRMAIAVILAVTLSVAAWSAPGADGRWLSRGVSGLTVTPAIIQ
                     KELGRFLWLPDTRVGQINVANRGTTPIVVDFQIAGIAHNADGSIDVLPCPPEVAAGLI
                     LSPAGPMTVMPDRAAAVRLELDRAYIDMPGRSGLCAALIISSAPMPARLASQGLEVGS
                     TIVLPVMVKLSWRDRARLAVVEACVESSGAENSRPALALKVANEGGAYARVDGRVSIT
                     AADGTRAAEAEFGPAVALPGCMRAFRMELEGPELPPGRYLVDARIRADGKAAGGAKFA
                     VEVGPKITVSSLGRP"
     gene            complement(7986..8525)
                     /locus_tag="PLM74_03580"
     CDS             complement(7986..8525)
                     /locus_tag="PLM74_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42198.1"
                     /translation="MITIPFSPRHGRNRSAIVRVALVLAFAVLTAWGMHANCQEECSL
                     VVQGRIAPAVSIYTSPDLIEWNDLYPGTNDLENAVTVTMLSNVDYEATISCSTPYMKG
                     PGLTELLTNPLEWKCSGGDYRVISVAPATIRLGGPSASGGDSELISFRQVVEYSDPPL
                     DNPAKAYVMTVRFAIEPRL"
     gene            complement(8598..9392)
                     /locus_tag="PLM74_03585"
     CDS             complement(8598..9392)
                     /locus_tag="PLM74_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HON42199.1"
                     /translation="MVIDSHAHLDDGAFSNEIDMVIARARAAGVGAIINPGGDVASSK
                     AAIANATAYPEVYALAGIHPHEATGIVDEAIGEIAEILSAPKVVGIGEIGLDYYRELS
                     PRDDQLRVFRAQMDLAREMGLPVEIHNRDAHSDILKIVQEYSDHIPAIVLHCYSGSAE
                     MARELVRIGCHISLAGPVTYRNARKQVEVAAQIPLDHLLVETDSPYLPPSPYRGQRNE
                     PAYVVEVVRKIAETRNTSFQDVAAATSANAAKIFGISPGRAMQDRP"
     gene            complement(9396..>10850)
                     /gene="metG"
                     /locus_tag="PLM74_03590"
     CDS             complement(9396..>10850)
                     /gene="metG"
                     /locus_tag="PLM74_03590"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF020892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HON42200.1"
                     /translation="IQPASRRHEMVNFIKGGLEDLCVSRTTFTWGIPVPFALGHVVYV
                     WLDALTNYLTAIGYVDDPERFARYWPADAHVVGKEIVRFHSVIWPAVLMALGLPLPKC
                     VFGHGWWTVEGEKMSKSKGNVIKPKEYADEFGVDALRYFVLREAPFGQDADFSRMSFI
                     YRVNADLANDLGNLLSRTTAMINKFAGGVVPATGEWIDMDRAIPELAGEVVEKAADAF
                     DRLDFQEGMAQVFRLVDAANKYVDEAAPWALARTDEGRRRLGTVLYNLAESLRIATVA
                     LSPVLTRKASEIWVQLGLSGDPADAGWDATRWGGMVPGTQIRRGEPVFPRFDTKAEAV
                     SVSAGKPADKPADKPADKAADKPADKPADKPADGPAPPESDLIGIEDFARVDLRVATI
                     LEAERVSGSDKLVKIQVDLGQERRQIVAGIARHYSPEELVGKQVVVVSNLKPATLRGE
                     VSNGMLLAASTGGDLKELSVLTLDRPIASGSKVK"
BASE COUNT         2258 a         3389 c         2959 g         2244 t
ORIGIN      
        1 tctacaggtc ccccagctgg cacctgtcac tccctactta aactttactc acccctcgat
       61 agcctgtcta gcagcccttc gatataggtt atcgtacatg cttgtcctga ttattcacgc
      121 aaccggtaac ggaaatcgcg aaatcgagag agcagggacg gcccgaggcc gtccctgccg
      181 cgcgtatccg tcgcgtatcc gttacacggt tgccatttcc gtcgatgtgc tgctgtgcgg
      241 ccctactgag agcgaggcct tgggaacgct ttccatacga ttgtgtgcgg tttcgcttgt
      301 gcatctggcg ccgctgcctg tttcggcctg ggcggttccc gctctactgc cggctctacc
      361 gccggctctg cttccggccc tgccgctggc ttgttcgacg gttccttcgg cggttccaca
      421 agcagatcta cgctcacacc cagggttgac tccgcgtcgg gttcgtgttc gccgcctggc
      481 tcaggcagga tctggtccgc gggcacgggc caggccatgt agttcgagtg cccaacgggc
      541 tcaagtccaa agtgctccgg cgaatagccc tggctacgga tgaaatgagc tgccgcatcc
      601 atctcctcgg gtcgcaaggg cacatcggcg cggcccgtgt cgccaggtaa agtcacctgc
      661 agcatcaccc ccctggcgtc agtcagttcc aatgggccgg cccatcatca ctatattccc
      721 gccagggttg tcacttgcct ctgagccgac agcttacttc gatttccccg cccgcccctg
      781 ccacgcgtgg agcgccaacg ccacagcgat gactccgtac gcaaggaagc tccggaaata
      841 ctcgccaacc tgggagaccc caaacaggcg ctgcccggcc aatggcgata ccacgaacaa
      901 tgtatggaaa agcagagtcc cgactatggc gttccatatg gtcgcgcgcc tggtcgtagc
      961 gccgcccacc aggatcgcgg cgactgagaa catacccacc tgttcatggc tgctgtacgt
     1021 gttgaacgtg ccgatattct gcaggaatat cagctgcccc cacgcagcaa gcacagtcga
     1081 catcattatc gcaatgatgc gcgttcggtc tacattgatc cccgccactt gggcgatgtg
     1141 catgtcctgc cccaccgccc tcatctcctg ccccagtttg gtacgggtga tgaaggtcgt
     1201 cagcacgcag agtatgccga tgataaacag cgtgcagatg ggaaaccgcc acgggcccag
     1261 cctggttcca ccgatcgagg tgaacacgaa gccgttccac agccagtcca ttgcgcgatc
     1321 cagcgagtag gaaaggccta tcaggtcaat ggtattcctc agtccaatac ctgaccccag
     1381 catgagcaca gggtccttca tggggatgag cgtgcccagc aagaccagga agactagctg
     1441 gtagatgccg ttagcgaaga agcccatgat catgctcgta accatctcgc gacccttggc
     1501 ctggttgagc gttctcccta caagccagcc caatagcatg gcgatgggta ccgagatgac
     1561 cgccgctagt gcaaagccgg ccaggccttc gatatggtag tgcgtcacga tcaccaaggc
     1621 tgtctgcccg gccatggcac caatcactat tccgaagtta agccccaacc ccgccatgac
     1681 cgggattatc agcgatatca ccaggaacgc attgcgtccg aatcgcccta ctacctcatt
     1741 tataaggtag ttaatatcca gccgtgccgc agcaattccg gcaatcgata tggcaaggaa
     1801 gaagatcggg actgaccagt tcctgagtat gctcgagatt cccgcgccca ggcctctagc
     1861 cggcgtctcg acctgtgcag ttgattgttc cgacataccg gctacctcct ttcagcccgg
     1921 gtgagggcgt agatgatcat tccgttgctc atgatcatgc gtgcaatttc gcttatgtct
     1981 gctccctgca gcaccgtctg ggtgaccggc aaggctatcg taagcagcgt gtgaaatagc
     2041 acggtcccga tgagagcgtg cccgatagtg gccttgcgaa gcgaagcgcc gccaatcaga
     2101 acagacgcga cggcagggaa tgccgccgtc agtggcgccg tatacagctg cacaaagccg
     2161 tagctctgtg aatacaccag gattcctatg gccgctagca ccgtggagag tgtcacgccc
     2221 agggtgcgca ttcgctgcac gttgataccg gaagacctcg catacatcgg gttagacccg
     2281 acggtgcgca ttgccaagcc cgctttggat ttgaaaaaca gggcaatcag gacgaagaac
     2341 acgcccatga acaacagaag ccctgtggga atatacacgt tgaagatgct gaacccaagg
     2401 aagtcattga gaaccttccc gaacgttcgg tcgagtgtga tcgtagttct caaacccttc
     2461 ccgcccagag cgaatatcat cgccggattg cggtagggcg caagcaacca gaacatgcac
     2521 atgccggata ccaccgaaaa ccctacgtag gtgccgacca tcatctcctg gccctgaaca
     2581 cgctcaagca gcatcgcgta gagatagccc gctatcaccg ctagaggtat ggcaatcgcc
     2641 attgccgcaa agaacccggc gaagccctta aggccccagt tcatgctggt cacagctccc
     2701 acaagaccgg ctatgacgcc cacgggaagg ccgaaattga ggccgactcc acacgccaga
     2761 gttggcagca tggccagaac cagaattccg ttcatgccta tcctggtgat cgagtccttg
     2821 atcaggatgg acaagtccat gcgcatgatc actgccagcg caaacagcga cagtagaaag
     2881 gcgatgatta tgagccgagg tagccccagt tgctcatacg cattcttggc aagttgccga
     2941 ataccggcct tggactcatc actcatgtta gctcacctcc tcgagcgtcg cgctcttgcc
     3001 ggccatcatc aggccgaagt caacatcgga cgcgtcgggc ggcagcacac cggcgatttg
     3061 cccacggtag actatggcta tcctgtcgca gatcttcctc agttcaccaa gctccgatga
     3121 cgtcatcacg atggtcatgc catgctccct attgaacttg accaataggt cgagcaccaa
     3181 ctgcttggcg cccacgtcta tgccgcgcgt aggttccgaa acccagagca cctcgggctc
     3241 catcgtgaac gcccgaccaa ggcacacctt ctgctggttg cctcctgaaa gccgtcgcac
     3301 aagctgagcc ggtcctgtgc acctgatatc aaggtcacga atcacggaaa gagcatgctc
     3361 tctcatggcg gcccgatcct caaggcgcag acttggcagc acgcctggct ggagaaactt
     3421 gccctgaact tgcatagccg tgagaatgat gttcatctct atggactcat ctagcaacaa
     3481 tcccacgcct ctacgatcct ccgatacgaa ggccatgccc gaaagcaacg catgtttcgg
     3541 gtcgttcaga ggtatccgga ccccatcttt ggtgaccaga ccgctcgctg gatacatgcc
     3601 tatcacgccg tttgcgatac ccaccttacc gtggccggca aggccgccta tgcccaggat
     3661 ttctccgcgt ctcaccgcaa ggttcagctc gcgtacgcgc tcaccaggca tgtctacctt
     3721 gaggttctcg attttcagga cgacatcctt gtcggacggc tccgacttcc tcggcggcag
     3781 aacaggcctt tctatttttc ttcccaccat gagctgggcg attctatcca gttttgcctc
     3841 gtcgcgaggc attcttgaga ctacctctcc gtcgcgaagt atggtgatgt tgtccgcgac
     3901 ctccatcact tcatcaagac ggtgcgtgat gaagagaatc gcgatgccct cggagctcag
     3961 ccgtttcatg gcggcgagca acaaagaggc ctcgctctca gtaagaacag cggtaggctc
     4021 atcaaacacg agtaaccgca cgttcttctt gtcgatctcg cgagctatct ccacaaactg
     4081 catatgcccc accggcatcc cgcctaccag cgccatctca tcgatggaga gcgctagctt
     4141 gcccagggcc actcgagaat cggattgcat caaaggctcg tccagtgttt ccagcttggg
     4201 cccgaagagg cggctcgcta cattgcttcg agtcggctca cggttgagct tgatgttttc
     4261 ggaaacggtg aaccccggta tcagcatgaa ctcctggtga accatgccga tgccgagttc
     4321 catcgctttc ctcggcgagt caatggtgac tggctgccca cccacaacta tctgaccctc
     4381 gaatccgcct gtatcgagta tcacctgcat tccgaagaga atgttcatga gtgtgctctt
     4441 gcctgcgcca ttctcgccca cgagcgcatg aatctcccca gggctcatac tcagattaac
     4501 gtccttcaga acagtagttc caaagaagct cttggtaata cccttgagct cgaggacatt
     4561 tccgctgccc atatcaagac ccctctcgat agtctgaaag ggcaatcacg cgcgggcgcc
     4621 gaacaaagcc cggcgcccgc cacgctatgc gctgcacacc acgatggtca gcaactgccc
     4681 tttctagtgc tccgctactt cacgtccttg ccaaatacga tcgagctaat cagatagcgg
     4741 tagtagcagc ctgtcgcgct gtgacgctcg aattccattg gcacgccggc gagctgctca
     4801 acaactttct tcacggcagc catgtccttg atgttgagct tcttctccac ggcgagcttg
     4861 gcaacttcca caccggccac ggtattcaga tagctggatg ccacaggcca ggtcgccatg
     4921 cgccctgaca ttcccctctt gactatctct gcgttgatgg ccttaactat cgcagggaag
     4981 ttgcccgcca tttgctcagt gatctccagg cccagagctg caggataccc atgagtcggg
     5041 ctcgggcagc actgctcggg gaagatcgct cctgccttaa gaaccgctgt gatcaagggt
     5101 tcctgcatac cgcagttggt agagaagaag gcggtatcct ttccgtactt ggccacctgc
     5161 cggggtacat cctccaggat gaactgctgg gccgcgggga gtccgccctc tcccatgggg
     5221 tcgggagcgg tcacgaaaac aaactgcatc ccgagtttct tgcactcctc atccatgatg
     5281 tcgcgcctgg cagccaggtc ggcataggac atgtgacgcg ggaacgagta gtgcaggaag
     5341 gtctttgcgc ccagcttctt ggccagttga ggaatggacc gaccacgagc tgggttattc
     5401 gggatcaagg aaaggtcggc taccgggtcg atcgttggcg ggtcttcatg aggttcgccg
     5461 atcacgaaga gaatgtccgg cctgagttgc ttgaccttac ggatagcagc cacagtgccc
     5521 ggcactccct ggttgaccac gatggccttg atcgccgggt cggcggcgag actggtgatc
     5581 tgagcgatgt atgtctcctg ctcggccatg aagttgtcag gatacgtacg gtgctcgatc
     5641 ataccagggt acttggcgac aacgaactcg gccccacgat attcatcttc gccctgtgat
     5701 actgtaccgg taacaacacc gatcttgaac gcaggcgcgc ccaacgatac gctagcgaca
     5761 ccgagaacca ggataagcgc agcaatcacc aataggcttt tcttaaacat ctaagcgctt
     5821 ctctcccttc cattcttccg gggttgccgt atatgcgaca cctaccccga agcataggtt
     5881 gaaagctatt attcgccttg gctctccagt ttcctgctta cagcccgacc ttgtgtttat
     5941 tccctgtggc tctctgcgcc cgcacaatcc cgatttccgc ctactccccg gacaggacta
     6001 atcgtcgata cgtagtaatt gtagtgtgaa acacgagcga tattgacttt gcggtccgca
     6061 tagaatggac atggattaca cgtttcatgg attacacgtt ttgagaggaa tgagcttgcc
     6121 atgagttctg ttgaggacag agaacgcgag ataatgttgg agttagttcg agtgaccgag
     6181 actgccgcgt tacgagcagc ccgctgcatg ggaatgggcg actgcaacct ggtggacgac
     6241 gcggcggtcg acggcatgcg cgggatgctg tcacttatca atatcaacgg tacgatagta
     6301 ataggcgaag gtgagaaaga ccaggccccg atgctctatt gcggtgagcg ggtaggtact
     6361 ggccgtgatg agtgcgatgt cgatctggcc gtcgaccctg tggaaggtac caggctcgtg
     6421 gccaacggct tgcccaacgc catatcggtg atggtcgtgg ctgatcgagg caccttgatg
     6481 ccggtgccca cattctacat gcacaagatc gcggtcggcc ccgaagctcg cgactacatc
     6541 gacataaacg cccctgtccg cgagaacctg cgggtcgtag ctgcagcttt aggccgcaag
     6601 gtcagagacc taacagtagt cattctaaat cggcccaggc atgaccagct catcagcgag
     6661 gtgcgcgagt gcggcgcccg gataaagctc atacaggacg gcgatgtcgc tgctgcgata
     6721 tcaactgccc ttcctaacac aggcgtcgat atgctgatgg gaataggcgg cgcgccggag
     6781 gctgtgctca cggcggccgc catcaagtgc ttaggcggcg aactccaaac gaagctctgg
     6841 ataagggatg acgaagatgc ggcccgtgcc cgcgaacgcg gcttcgatga tatggagcgt
     6901 gtgtattgtt ccgaagactt ggcacgcggc aatagcatag tatttgccgc cacaggcatc
     6961 accgacggtg atatgctgca gggcgttagg tactacggcc agaaggccac taccgagtct
     7021 attgtcatgc gcatgctgac tggtaccgtc cgccgcatac ataccacaca cgatctttcg
     7081 cgaaagactc tcaggtcgtt caaggccggc caagagatga taccgtgatt ttcgggccga
     7141 cttccacggc gaatttggcg cctcccgcgg ctttgccatc ggccctaatc cgcgcatcga
     7201 cgagatagcg gccaggcggc aactcggggc cctcaagctc catgcgaaat gcccgcatgc
     7261 accctggcag cgctaccgcc ggcccgaatt cggcctcagc ggcacgcgtt ccgtcggcag
     7321 ccgtaatgga cacccgccca tcgacccttg cgtatgcgcc gccctcgttg gcaaccttca
     7381 gcgccagcgc agggcggctg ttttccgcgc ctgagctttc aacacacgcc tccaccactg
     7441 caagccgggc acggtcgcgc cacgacagct tgaccattac gggaagcact atcgtgctgc
     7501 cgacctccag gccctgcgac gcgagccggg cgggcatagg tgcgctcgag atgatgagcg
     7561 ccgcacagag cccactccgg ccgggcatgt cgatataggc ccggtcaagc tccagcctga
     7621 ccgctgcggc tcggtcgggc attacggtca tcggccccgc cggcgacaga atgagcccgg
     7681 cggcgacctc gggtgggcag ggcagcacgt ctatgctgcc atccgcgtta tgcgcgatcc
     7741 ccgcgatctg gaaatccact acgatcgggg tcgtgccgcg gttcgccacg tttatctgcc
     7801 cgacccgggt gtcgggcagc cacagaaacc gacccagctc tttctggatg attgcgggcg
     7861 taacggtcag ccctgatacg cctctagaaa gccatcgacc atctgccccg ggggcggacc
     7921 atgccgcgac agacagtgtg acggcaagaa ttacggcaat agccattcgt gtcatcgatg
     7981 ttcgcctaca gccgtggctc aattgcaaac cgaactgtca tcacgtatgc cttcgccggg
     8041 ttatccaaag gagggtcgga gtattctaca acctgacgga atgagatgag ttccgagtct
     8101 ccgccgctgg cgctgggccc tccgaggcgt atcgtagcag gcgccacaga tatcacccgg
     8161 taatcgcctc cactgcattt ccattcgaga gggttggtca gcaactcggt gagcccaggg
     8221 cccttcatgt acggtgtgct gcagctgatt gtggcctcgt agtcaacatt gctcagcatg
     8281 gtgactgtga cggcattctc gagatcattg gttccggggt acaagtcgtt ccactcgatt
     8341 aagtcgggcg aggtgtagat actgactgcg ggcgcgatcc ttccctgaac tacaagactg
     8401 cactcttcct ggcagtttgc gtgcatgccc catgccgtca gcacagcaaa cgccaagaca
     8461 agcgcgacgc gaacgatggc cgaacgattt cggccgtgcc tgggactaaa cgggatcgtg
     8521 atcatacgcg aaacccctcc ttgatacatt tgtcaagaag gggcattccc gctggtgcca
     8581 tattattcct ctcacactta agggcgatct tgcatcgccc gacctggtga tattccgaag
     8641 atcttagcag cattggccga cgtcgccgca gctacatcct gaaatgaggt gttcctggtc
     8701 tccgcgatct tccgcaccac ttccacaaca taggcaggtt cgttccgctg tccgcggtac
     8761 ggactcggcg gaaggtatgg agagtcagtc tccaccaaaa ggtgatctag tgggatctga
     8821 gcggcaacct ccacttgctt tcgcgcgttc cgataagtga cgggcccggc cagagagatg
     8881 tggcacccga tcctcaccag ttcacgcgcc atctctgcgc tgcctgagta gcagtgcaag
     8941 acgatcgccg ggatgtgatc ggagtactcc tgcactatct tcaggatgtc gctgtgggcg
     9001 tcacggttat ggatctcgac aggcaggccc atctcccgcg ccaaatccat ctgcgcacga
     9061 aatactcgca gctgatcgtc gcgcggcgac agttcgcggt agtagtccag gcctatctca
     9121 cctatgccta ctactttagg agccgacaga atctccgcga tttcgccaat cgcctcgtct
     9181 acaattcctg tcgcttcgtg cggatgaatc ccggccagcg cgtagacttc gggatatgcg
     9241 gtcgcgttcg cgatggccgc tttcgatgac gcaacatcgc cgccggggtt tatgatggcc
     9301 cccactcctg ccgcccgcgc cctggcaatg accatatcga tttcgttcga gaacgcgccg
     9361 tcatccaggt gcgcgtggga gtcgataacc aatctctact tcaccttgct cccggacgca
     9421 atcggccgat ccagcgtaag cacggaaagc tccttcaggt cgcccccggt agacgctgcc
     9481 agcagcatgc cattggagac ttcacctcgc agcgtggcag gcttcaggtt ggaaaccacg
     9541 acaacctgct tgcctacgag ttcctccggc gagtagtgcc gggcgatccc cgcaacgatc
     9601 tgccggcgct cctgccccag gtccacctgg atcttgacta gcttgtcgga tccggatacc
     9661 ctctcggcct ccagaatggt agccactcgc agatccaccc tggcaaagtc ctcaatgcct
     9721 atgagatccg actcaggcgg cgcaggccca tctgccggct tatcggccgg tttgtccgcc
     9781 ggtttgtctg ccgccttgtc tgccggctta tccgccggct tatccgccgg tttgccggcc
     9841 gataccgata cagcctcggc cttcgtatcg aaacggggga acaccggttc gccgcggcga
     9901 atctgggtgc ccggcaccat gccgccccag cgcgtcgcgt cccatccggc atctgcagga
     9961 tcgccggaaa gaccaagctg cacccagatc tcgctcgcct ttcgggtcag tacaggagag
    10021 agcgctacgg tagcgatgcg gaggctttca gccaggttgt acagaaccgt gcccagcctg
    10081 cgccggccct cgtccgtcct ggccaatgcc catggagcag cctcatctac atacttgttg
    10141 gccgcgtcga ccaggcggaa gacctgagcc atgccctcct ggaagtcaag cctatcgaag
    10201 gcgtccgccg ccttttcgac aacctcgcct gcaagctccg gaatggcccg gtccatatcg
    10261 atccactcgc cggtcgctgg tacgacgccg cctgcgaact tatttatcat cgcagtggtt
    10321 cggctcagca gattacccag atcgttggcg agatcggcgt tcaccctgta gataaaggac
    10381 atgcgagaaa aatctgcgtc ctggccgaac ggcgcctcgc gcagcacgaa gtaacggagc
    10441 gcatccacgc cgaactcatc tgcgtattcc ttcggcttga tcacgttccc cttggacttg
    10501 gacatctttt cgccctcaac agtccaccag ccgtgcccaa acacgcattt gggcaagggc
    10561 agacccagcg ccatgagcac agcgggccat atcaccgagt ggaacctgac gatctccttg
    10621 cccactacgt gcgcatcggc cggccagtac cgcgcaaatc tctcaggatc gtccacgtat
    10681 ccgatggccg tgaggtaatt cgtaagcgca tcaagccata cgtacactac gtgacccagg
    10741 gcgaacggca ccgggatgcc ccacgtgaag gtagtacgcg acacacacag gtcctccagc
    10801 ccgcccttta tgaagttgac catttcatgg cgcctgctgg ccggctgaat
//
LOCUS       DAOTAC010000052        10708 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_5264_length_10708_cov_5.065803, whole genome shotgun sequence.
ACCESSION   DAOTAC010000052 DAOTAC010000000
VERSION     DAOTAC010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 10708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10708
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5264_length_10708_cov_5.065803"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(801..1082)
                     /locus_tag="PLM74_03595"
     CDS             complement(801..1082)
                     /locus_tag="PLM74_03595"
                     /inference="COORDINATES: protein motif:HMM:NF017416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HON42201.1"
                     /translation="MGIKNSSGKLLYRFSLEDRVPDKHLLRSIREAVGFSFIRRIAEP
                     FYSHIGAPSAEHIVALKMALLGYLYGIARQPKLAEECRLNMAFVLVPRP"
     gene            1280..2113
                     /locus_tag="PLM74_03600"
     CDS             1280..2113
                     /locus_tag="PLM74_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42202.1"
                     /translation="MKKRLLVLGLALLLAVVWTSLVSAAELTLGGGIVYQLGSYGFTF
                     SNGDEEAPYDCSASAPPLRYELSLAYGRWNLNGFLTKQILGNGTVDEDLVELEENLAY
                     YGGVTAFGGDVAYLFDIGSFKLGPSIGYRSTQVISGVKSSDETGFGAEGIGFQTRGLR
                     LGAYTEAALGDRFSFGASLGCSPRLGVAEISEGRFSDTEDHMHGYYSQVHEIPAPSKA
                     FSFDLAVTASVRVMPAIEIVGGYRYEHASIELDPESSSHNVSAYTMNASVLSVGARYR
                     F"
     gene            complement(2575..2982)
                     /locus_tag="PLM74_03605"
     CDS             complement(2575..2982)
                     /locus_tag="PLM74_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HON42203.1"
                     /translation="MNVYAYPAVLQPEDVGGYSVEFPDLLGCCTEGDSLAEAVVMARD
                     AVGLYLYSLEEHGDHIPPASSLDTISTDPGSFASLIDIDMLAYRQKHDNRAVKKTLTI
                     PAWLNTMAEANNINFSQTLQSALKEQLGLTDRL"
     gene            complement(3005..3205)
                     /locus_tag="PLM74_03610"
     CDS             complement(3005..3205)
                     /locus_tag="PLM74_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004831652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HON42204.1"
                     /translation="MKPRELIKLLESDGWYLDRVQGSHYAMRHPNKPVQPVIPYNNKD
                     LKRGTLAKIRKQAGLDNPACGN"
     gene            complement(3380..6268)
                     /locus_tag="PLM74_03615"
     CDS             complement(3380..6268)
                     /locus_tag="PLM74_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42205.1"
                     /translation="MSHVVSGDGWRPLPYWVSPARPFYPSVGLEKLWPRLFVDEAIHI
                     ASQMVAILLHLDQHVHVLRNYRIYERRLGTQGLSPVGHALRGRKSAPTGESPNQSQRA
                     RYFASGGTSHMAEKARIYLAAVFICLAFIAHPDRSQAQATYATEFDVALTVTSITKSE
                     SFDGWGELSKRSQRNESYSTDLIMSVYRNKLDRYIEDPFKALKNVDYRTTTMFEIASG
                     SNSVEDSAGKSSRFPVKVNISSRLWERSIEGKDKGLVLTYVYDGQGTLDKTGVGLHIS
                     PIPPGSGKGPVVSLVSGYRLVVSAGNIQSFTDPSAPRAKARYYDQYLKKWIDDPQKYG
                     IAPSSHWVLSNTHLDPEIGEDATVKLAEFPNPWELQKFLTDPKGSATFTVKASYYVDN
                     SSEKSTTDILVTLTLTPRPQKLEAVIYGPSGYEEWLPKGGRDEKSAGNGVVITTRLQL
                     ENDPLEFPMEKVQFRYELVDTSKERGVCLNSPGLDLAAAMSGSLDDPEYDLKISAKNN
                     PHLEVSEDGQTATTKEQAINPQFPSISSVRVDCFDWGAHAKLKVTAVLEDGTEIPAHV
                     NGEPDKTEFAIPIDDNDNYVADAWERQMKVFGNNLPAAWDESPHPQGQRANGDGISLY
                     EKYRGLYCSKLGTGSSGAGSLTHERLDPNHKYLFVCDENGILGAILHDPVAEAAARSF
                     EALSGLRIRLVSGSYWTGPGSIGDARRIVNFNNGDDTNAVEQTALHVTVTNDYNLVPP
                     GWLEFWESSRGEPLIVGVFDTWQYSSGYAFPDIGGGKIGSPKYTYQINISLGLLDYNL
                     KGSAAAHVRRQDWACEEDDSIERDFQEWMDEHPHTYEDFHPEPGQSTELLDAYEARRE
                     QQKQEHEALEERIKQAVEDYIDAHPDEWQRRRAIQIAYVLAHELGHGVGMSHHADDSG
                     DAMCIMTVFQCDYNTDPYDVLDARQLDKIPNKICPSCVRQIVVSDGKE"
     gene            complement(6895..7259)
                     /locus_tag="PLM74_03620"
                     /pseudo
     CDS             complement(6895..7259)
                     /locus_tag="PLM74_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005345391.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
     gene            complement(7289..7393)
                     /locus_tag="PLM74_03625"
                     /pseudo
     CDS             complement(7289..7393)
                     /locus_tag="PLM74_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674407.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            7611..8999
                     /locus_tag="PLM74_03630"
     CDS             7611..8999
                     /locus_tag="PLM74_03630"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HON42206.1"
                     /translation="MRRTAVITLATLLLLSCAVAEAKPSSEPCPVHAGAWSSVGQLRS
                     LIATGADVNEVDELGDTMLISALRFGLGQDFVTVLLQAGADVNATSMVGETVLMLALQ
                     SQHGLDVVTALLQAGADVNAKDATGRTPLLYAAMYSPNPRIIQALLNAGADPRAKADD
                     GWTAFDAAGYHDSIRNSDVYWLLKPDGTTARPSLLDLGSWRTADELRSLIEAGADVNE
                     ADEAGNTPLMLALSIGHKTNVIKALLEAGADVNARDENGTTPLVSAVMRDYGPDVITA
                     LLDAGADVNAGTTEESWSAPRGQTALMYATISARVDVMNALILAGADVDAADGNGDTP
                     LTTAAMYSSVSGYVDAVRTLIAAGADVNVRTKAGTTALMRASEDGGIDAVRALIAAGA
                     DVNAKDKLGWTPLIFAVSRVSNPEVIRALLNAGADRRAKTDMGKTAFDLAGLNSNIKD
                     TDVYRLLKPNTP"
     gene            10520..>10708
                     /locus_tag="PLM74_03635"
     CDS             10520..>10708
                     /locus_tag="PLM74_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HON42207.1"
                     /translation="MITAQGLTKRYGDVLAVDNVDLQVNAGEIFGFLGPNGAGKTTAI
                     NMLIGITSPTSGAVRLGGV"
BASE COUNT         2159 a         3011 c         3182 g         2356 t
ORIGIN      
        1 ccttttagct ggtccgatga taaatgccgc tggggactgc gcccagcggc attcgtattt
       61 gtctgcgaaa tgtgtgcgaa actccttcgt gtgtgcaagg cttatttccg gtccccgcca
      121 agaagcatgc tctcaagttt gtttacagcg ccctgttgca tgtcgggaag cacatgggag
      181 ggcatacgga atcaggggca agccttgcgg tcgtgcatct ggttcatgtc gctttcttct
      241 ttgtagccag gcatatcgcg gggtagcatt ccgatcatgt tcatacgccc aacaagcgaa
      301 acctagacgc caaacgaatg acaacgaccc gtttttggcg tctgtctcat agacaaccgc
      361 gtcgtacgat ggcgcgtgaa cgggttaagg tcgacctaag cgcagaggtt atgaagatca
      421 agccttctga caggagcgat cataatgcgc agtgttggaa tcgcgttgct cgtcctagca
      481 tgctcggctt gtctgaccag tggatgcgac agttcaaaat gctgactaat ccttatgctg
      541 atgcagctga catggctcgt atcgtgtaca tggttgcttg ctctgctctg gagttgctca
      601 tatagaactc cgttggccag tgagtccaat gatgcgttgg cctgcaagcg gatagaatcc
      661 atatgcagag tgccgccttc ttcaacccgc aggcctgaca ctacctgacg atctccctga
      721 agaacttggc gtagacagcc tttccaaacc gctgtcgata cttcgatagt atgctatggt
      781 cacggggaag ctcatcgacg tcatggccta ggaaccaaaa cgaacgccat gttcaacctg
      841 cactcttcgg ccagcttcgg ttggcgtgcg attccataga ggtaacctag tagagccatc
      901 ttcaaggcca cgatatgttc agccgatggc gccccaatat ggctgtagaa aggttctgcg
      961 attctgcgaa tgaacgaaaa gccgactgcc tcacgtatgg atctgagcaa atgcttgtca
     1021 gggactctat cttccaggct gaaccggtac aacagcttac ctgacgagtt cttgatcccc
     1081 atcatcatgc atcacctacc gctggcagta gcctccacgg ctattctgcc agcctgacgg
     1141 gtgtttatgt gcgagtttgg cgacagacct gctagcatac acgtccacct gtcacaacca
     1201 agaaggagtg cactctccca tgtcgaaaat actcaaacag ccatctgctg gggccaaagg
     1261 ccctgaaagg aagagtgcaa tgaagaaacg tctactagta ctagggctcg cgctactgct
     1321 cgcagttgtc tggacaagcc tggtcagtgc tgcagagctt acgctcggcg gcgggatagt
     1381 gtaccaactg ggaagctatg gcttcacgtt cagcaatggc gacgaagaag cgccctacga
     1441 ttgttccgct tctgcacccc cgcttcggta tgagttgagt cttgcatatg gacgatggaa
     1501 tctgaatggc tttctcacga agcagatcct gggcaatggc acggttgatg aagacttggt
     1561 ggagctagaa gagaatctcg cctactacgg aggagtaacc gccttcggtg gagacgtcgc
     1621 ctatctgttc gatatcgggt cattcaagct tggtccttcc atcggctacc gaagcactca
     1681 ggtcatttcc ggagtcaaga gttcggacga aaccggcttc ggagctgagg gcattggatt
     1741 ccagactcga ggcctcaggc ttggcgccta tacggaggca gctctgggcg acaggttctc
     1801 atttggggct tcacttggat gttctcctcg ccttggcgta gctgagattt ccgagggcag
     1861 attctctgac acagaagatc acatgcatgg atactacagc caagttcacg agatcccggc
     1921 gcccagcaag gccttctcgt tcgacctggc agtaaccgca tcggttcggg taatgccagc
     1981 aatcgagatc gttggcgggt atcggtatga acacgcctcc atcgagcttg atccggagtc
     2041 aagctcgcac aacgtgtcgg cgtatacaat gaatgcaagc gtgttaagcg tgggtgcaag
     2101 gtacaggttc taggctgttc caggggtatt tccacaaccg aatagtgcta agaagccgcc
     2161 ggcgcacggg atttcaccgc ccggcggttt ttccgctttt acgcccttct agctctacac
     2221 gttacccaca tcgctggaac gaatgcacat cttgcttata gaatagagta gataccttgc
     2281 ccgagggact cgccctgcgc cagcgagcgc atgccggtag ctcgaatcga tttgggcgcg
     2341 atgccttggc ggatacccgc atggggtatc tgtgcagctc ttctcccctt ctgaggcgtt
     2401 ggacggagat ttgccgcact catacggcga aaactctctg cgttgggccc cggagatcgc
     2461 aaacgcttgc aggcccagct agagcggcta gaacgcggaa actccaggag gattcactct
     2521 agaccgccgg agcgggccca ctctttcccg cctcgccccg cccaagcccc gtggctacag
     2581 ccggtcagtc agcccgagct gctctttgag cgctgactgc agagtctgtg agaaattgat
     2641 gttgttggcc tctgccatgg tgttcagcca tgcagggatc gtgagcgtct tcttcacggc
     2701 gcgattatca tgcttctgac ggtatgccaa catgtcgata tcgatcagcg aagcgaagct
     2761 gcccggatca gtacttatgg tatcgagcga cgacgctgga gggatatggt cgccgtgctc
     2821 ttcgagcgaa tagaggtaca agcccacagc gtcgcgggcc atcactaccg cttccgcaag
     2881 gctatcacct tctgtgcagc agccaagaag gtctgggaac tcgactgaat atccgccaac
     2941 gtcctccggt tgaagtactg caggataggc atagacgttc atgctgatac ctccgtttct
     3001 tgggtcaatt gccacacgcg gggttatcaa gcccagcctg cttccgaatc ttggccaggg
     3061 ttccacgctt aagatctttg ttgttgtagg ggattaccgg ctggacgggc ttatttggat
     3121 gccgcatggc atagtgcgaa ccctgcactc tgtccaggta ccagccgtca gattccagga
     3181 gcttgatcag ttctctcggt ttcaacttgc cccctcccag tacgccaaga atagcacgtg
     3241 tcaatacgta tgccaatggc acctgggtgg cggcaatacg ggatggcgca ttgagaaccg
     3301 cactgaaagg cagctcctgg tgcagtgcga ttcgtcatcc ttctacgccg tggacaacaa
     3361 caatcatttc ccgcccgtgc tactccttcc cgtccgacac gacgatctgc ctcacgcagc
     3421 tcgggcagat cttattcggg atcttgtcga gctggcgggc atccagcacg tcgtaggggt
     3481 cggtgttgta gtcgcactgg aacactgtca tgatgcacat ggcatcgccg gagtcatctg
     3541 catggtgcga cattcctacg ccgtgaccca gctcgtgcgc cagtacataa gctatctgga
     3601 ttgcgcgccg ccgctgccac tcgtcggggt gggcgtcgat gtagtcttcc acagcctgct
     3661 tgatgcgctc ctcaagagcc tcgtgctcct gcttctgctg ttcccttcga gcctcatagg
     3721 cgtcgaggag ctcggtggac tgccccggtt cggggtggaa gtcttcatag gtgtggggat
     3781 gctcatccat ccactcttgg aaatcccgtt cgatgctgtc gtcctcttca catgcccagt
     3841 cttgccgtct gacgtgcgcc gccgcacttc ccttcaggtt atagtcgagt agtcctaagg
     3901 agatgttgat ctggtaggtg tacttgggtg acccgatctt ccctccgccg atgtcgggga
     3961 aggcatatcc gctgctatac tgccacgtgt cgaacacacc cacaataagt ggctcgcctc
     4021 tgctggactc ccagaactcc agccacccgg ggggtacgag gttgtagtcg ttggtgacag
     4081 tgacatgcag cgctgtctgc tcaaccgcat tcgtatcgtc gccgttgttg aagttgacta
     4141 tgcgccgggc atcgccgatg ctgcctgggc cggtccagta gctgcccgac accagtcgta
     4201 ttctcagccc tgagagggcc tcgaaagagc gcgccgccgc ctcggcgacc gggtcgtgca
     4261 ggatggcgcc cagaatgccg ttctcatcgc acacaaacag gtacttatgg ttgggatcca
     4321 accgctcatg ggtcagagaa cctgcgcccg agctgcctgt gcccagctta ctgcagtaaa
     4381 gaccgcggta cttttcatat agcgatatcc cgtctccgtt cgccctctgc ccttggggat
     4441 ggggtgattc gtcccaggct gcaggcaggt tgttgccgaa caccttcatc tgcctctccc
     4501 aggcgtccgc tacgtagttg tcgttatcgt ctatgggaat agcgaactcc gtcttgtcgg
     4561 gctctccgtt tacgtgagcg gggatctcgg tgccgtcctc aagcacagcc gtgaccttca
     4621 gcttggcgtg ggcgccccag tcgaagcagt cgacccgcac cgagctgatg gaggggaatt
     4681 gggggttgat ggcctgctcc ttcgtggtag ccgtttggcc gtcctccgac acttcgagat
     4741 gagggttgtt cttcgcgctg atcttcaggt cgtattccgg gtcgtcaagg ctgccgctca
     4801 tcgccgccgc cagatccaat cccgggctgt tcaggcacac tccgcgctct tttgacgtgt
     4861 ccaccagctc atagcggaat tgcaccttct ccatggggaa ctcaaggggg tcattctcta
     4921 gctgcagcct tgtggtgatg accacgccat tgcctgcgga tttctcgtcg cgcccaccct
     4981 tcgggagcca ctcttcgtac ccggacgggc catagattac tgcctccagt ttctgcggtc
     5041 gaggggtcag ggtgagggtc accaatatgt ctgtcgtcga cttctcgctg ctgttgtcga
     5101 cgtaatacga tgcctttacg gtgaatgtag cactgccctt gggatcggtc aggaacttct
     5161 gaagctccca ggggttgggg aactctgcaa gcttcacggt tgcatcttcg ccgatctcgg
     5221 ggtcgagatg ggtgttcgac agcacccagt gagacgaggg ggcgataccg tacttctgag
     5281 ggtcatcgat ccatttcttg aggtactgat cgtagtagcg cgccttggct ctcggggccg
     5341 atgggtccgt aaagctctgg atgttgcctg cggataccac cagcctgtag ccgctgacga
     5401 gactgacgac gggccccttg cccgacccgg ggggtatggg gctgatgtgt aggcccacgc
     5461 cggtcttgtc gagcgtgccc tggccgtcgt agacgtaggt aaggaccagg cctttgtctt
     5521 tgccctcgat gctccgctcc cataatcggc tggatatgtt cactttcaca gggaaccggc
     5581 tgcttttgcc ggctgagtct tctaccgagt tggagcccga agcgatctcg aacatagtag
     5641 tcgtgcggta gtcgacattc ttcagggcct tgaagggatc ctcgatgtag cggtcaagct
     5701 tgtttctgta gaccgacatt atgaggtcag tgctgtatga ctcgttccgc tgcgaccgtt
     5761 tcgacagctc gccccagcca tcgaaggact cgctctttgt gatgctcgtg acggtgaggg
     5821 cgacgtcgaa ttcggtggca tatgtagctt gggcctgaga acggtcgggg tgggctatga
     5881 atgccaggca tatgaagact gccgcaagat agatccgcgc tttttcggcc atgtgagagg
     5941 taccccctga tgcgaaatag cgtgctcgct gcgactgatt cggggattcg ccggtaggcg
     6001 ccgatttcct cccgcggaga gcgtgcccga ctgggctaag gccctgtgtg cccaggcgcc
     6061 tttcatagat gcggtagttg cgcagaacat gcacgtgctg atcgaggtga agcaggattg
     6121 ctaccatctg actcgcgata tggatggcct catcgacaaa aagacgaggc cacagcttct
     6181 ccaaccccac gctcgggtag aagggccttg ccggcgaaac ccagtatgga agcgggcgcc
     6241 atccatcgcc cgagaccacg tgactcacag gatgtcatac ctcgacgtgt ttgcggaacc
     6301 gctggtgctg taacttcggt ggttgacctt cggcagataa ccgtggcaca tagcctagag
     6361 gattgacgac tgaatttgac gagtacctgc aatcggcggt gtacctgaag aaggccgctg
     6421 gacacgctct cagccgaaga cgttgtctta ggtcacaagc agctcctcga agtagaggcg
     6481 gcgtttcgaa cgctcaagtc gtcgccggag ctcaggccta ggtactatcg cgtttccaac
     6541 cggatcaagg cgcatgttcg catctgctgg ctggctcttc tgctggtcag gatagcggag
     6601 atcaaggtag gcaagtcatg ggcgaacacc agaagccaac tcgaaaagat acccattgct
     6661 gagatcggga ctcccgacgg acgaattgta cagcacaccg agatcagcgc agcttaggag
     6721 gccattctga ttcgtccgga ggtcagcgaa cccaagagaa ttcatggaat cagcctcaga
     6781 acgaggcctg gcgcgtagta acacgactct tcgaccccgt gcttccattg gccgatctat
     6841 ctgctgctac gcgcattgtt tgcctacgcg gtgtcgaact cgagaaatcg tgagctaccg
     6901 gcgttcgtga gctatgtcga gctggtgctt tagggcctcc tgaagtgtac gcgagaagtt
     6961 gatcccgtgc tcgatggcca gatcgtctag ccatttgggt attgtcaggg tcttcttgac
     7021 ggctcggctt tctatggcat cacgtactag aggcatccat gcgtcgatca gtatgagaac
     7081 ctgtcgccgt cctgctccat tccgtacagg tgcaatccca gcgcctgtcg ggcattttgg
     7141 agcgcttcct cggtggaacc tgcgcaaggg aggcatcccg gcagatccgg gaactcaatg
     7201 gatatgccgt cctcggcata ggttaggacg gccggaaaga tgtatcggtc tggtcccatg
     7261 gctgagtcac tgcccttcgc tctggggttt agaaccggac gccagactgc ttttcaatgg
     7321 ctctcagagt cccaatcggc acgtccttca ccgggtgcgt gactgtgatc ctgcccttcc
     7381 tggttggatt tcgcactgca cacacccttt ggcctgacgc caacacgtgt gaaggaacgt
     7441 gttaagggga cagccgtgga atcgaaggcg gaatcgagtt atcctgcagg tgatctgctg
     7501 cgcgcgggca gacggaagga caattagcgc cccaagacga actgtgcatg tcactgggta
     7561 cttgtggagt acgcatcgcg caccatgata tggaaaaggc ggagatacac ttgagaagaa
     7621 ctgcggtgat caccctcgcg actctcttgc ttctctcctg tgcggtggct gaggccaagc
     7681 cttcctccga accttgtccg gtgcatgccg gcgcgtggag cagtgtgggg cagctgcgct
     7741 ccctgatagc gaccggcgct gatgtgaacg aggtggacga gctgggcgac acgatgctga
     7801 tatcggcact cagattcggg ctcgggcagg attttgtcac cgtgctattg caggcgggcg
     7861 ccgacgtgaa tgccacgagc atggtcggcg agacggtgct gatgctggca ctccaatccc
     7921 agcacgggct ggatgtcgtc actgcgctgc tgcaagcagg cgcggacgtg aatgccaagg
     7981 acgcgaccgg ccggacgccg ctgctttacg cggcgatgta cagcccgaac ccaagaatca
     8041 ttcaggccct gctcaatgcg ggggctgacc cgagggcaaa ggcagatgac ggctggacag
     8101 ccttcgatgc cgcaggttac cacgatagta ttaggaactc ggatgtgtac tggctcctga
     8161 agccagatgg cactactgcc aggccttcac tgctggatct cggatcgtgg cgtactgcag
     8221 atgaactacg atccctgatt gaagcaggcg ccgatgtgaa tgaagcggat gaagccggca
     8281 atacgccgct gatgctggca ctgtcaatcg gccacaagac gaatgttatc aaggcgcttc
     8341 ttgaggcagg tgcagacgtg aacgctcggg atgagaacgg cactacgccg ctggtgtcgg
     8401 cagtgatgcg tgattacggg ccggatgtca tcacggcgct attggacgcc ggcgccgacg
     8461 taaacgcggg cacgaccgag gagagttggt ccgcgccgag aggccagacg gcgctgatgt
     8521 acgcgacgat atccgcgcgc gttgatgtca tgaatgccct catactggcc ggggctgacg
     8581 tggacgcagc ggacggcaat ggcgacacac cgctgactac ggcggcaatg tacagctcgg
     8641 tatctggata tgtcgatgcc gtccggaccc tgatagcggc cggcgccgac gttaacgtgc
     8701 ggaccaaggc cggtacgacg gcgctgatgc gcgcatcgga ggatggaggt atcgatgccg
     8761 tgagggccct gatagcggca ggcgccgatg tcaatgccaa ggacaagctc ggctggacgc
     8821 cgctgatttt cgcagtgagc cgtgtatcga acccagaggt catccgggcc ctgctcaatg
     8881 cgggcgccga ccgcagggca aagacggata tgggcaagac ggcgttcgac cttgcgggac
     8941 tcaacagcaa catcaaggac acggatgtgt atcggctcct gaagccgaac accccctagg
     9001 ggagacgggg agtccggctg cgttagtcgt ccatggatcc catagctcca atctgcgctg
     9061 ggacagatgg ttcgtgtcgc aggcactgga cgctgatgga gcgtcagttg caccataggg
     9121 acacgagtgg ggggcccatg gcattgttca aagcgttgtt cagagcgttg acgacgagga
     9181 acctgtcgct tttgggtatt cagaatcacg cccttcagga caaccctgat acgtgcgaaa
     9241 cgacgcgcca agctgggtcc cccggtgggg tgtgtgtaag gactgtggct ttcggggtcg
     9301 cagacccccc cgcattcagg cccgtggtct cgccagggcc atagcggacg cggccggaat
     9361 cgtaccgggt ccaggggttc atggatagaa ctgcgggtaa atccagcggt aaacccgtgg
     9421 gtaggtccag gagtaaatcc gcgggtagat ccaggggtac gcccgcgtgt aggtccagga
     9481 atgggcccag gggtgaatcc ggggatacat ccgggggcgt gctgagggag gaagagagga
     9541 ctgctgtggc tcagggatat acccccgggg gtatctgccc ggagatacgg tcacttttta
     9601 acaatacttt cgtcgaaaaa cgcaattttt gagtacatcg atttgggccg ggggccccgg
     9661 gaggcggatc gaccccgcaa aaggagccct cgcggctaga tatcgccgat ttagtggcgc
     9721 atcgctgtca ctacatgcca tcacttgcgc gctgttacca gatgaagcca tgatgtggga
     9781 tattatgtcg gcccactcgc ctgctccacc gccccgaccc gtcggatacc ccccgatttt
     9841 cgtgattggg aggcggtcag gcggctcttc gaaatcgatg tactcaaaag ttgcgggtat
     9901 ctccaaaact aatggcaaaa atcgacgata tgccagctcc agccgttttg tccggcggcc
     9961 atgatgctaa gaccagctaa acagccattc cggcgaggtg agcatgaccc ccccagccaa
    10021 caacccaccg tgatgatcgg ggagggtgtt ttctcgctca ctatcccgcc tggcgatatc
    10081 cacctgcttt tccaattgct attgcccacg atacatacac gcaccccccc cggtgcggtt
    10141 gctctccaag ctgtgccttc gccagggtca aatccaaagt ggctgggaga gaaggcgacc
    10201 cgagggtaac cccgaggaac ccgagggtat cctggcatgc acgccgtcgg tctttggcga
    10261 tagtctcgcc aaaaaacgct cgcgctccaa ctccgcgacg cctgcaagag ccacgcagat
    10321 ccggcgtggt acccatggga aggccttggg taggcctacg ccaggggtag ggccatgtta
    10381 ggggtactca cgtggtacct cacgtgggag gtactcccgt gggatgttga ctgaccgacg
    10441 caagtagtcc atactctatc cgaatctatg cggacttgcc ggcgccctgg gcgcctgtca
    10501 cgcattggag ggatgggctt tgatcactgc acagggattg accaaacgct acggggatgt
    10561 gctcgccgtc gacaacgtag acctgcaggt gaacgcaggt gagatcttcg gatttctggg
    10621 cccgaacggg gcaggcaaga ccacggctat caacatgctc atcggcataa cttcccctac
    10681 atcgggcgct gtgcgcctgg gtggagtc
//
LOCUS       DAOTAC010000053        10529 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_5370_length_10529_cov_4.785373, whole genome shotgun sequence.
ACCESSION   DAOTAC010000053 DAOTAC010000000
VERSION     DAOTAC010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 10529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10529
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5370_length_10529_cov_4.785373"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..427)
                     /locus_tag="PLM74_03640"
     CDS             complement(74..427)
                     /locus_tag="PLM74_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42208.1"
                     /translation="MDTPISQEAEMFSAEALNRHPIGAFIAQCPDPCQPVAVEFECGH
                     CCKKACNARITGICGGFLALAAFNPCPGIIIKLFCGGGVVNTEYAQCTLIRLDKISNI
                     EIGLTVNAAPTCPCP"
     gene            complement(597..2204)
                     /locus_tag="PLM74_03645"
     CDS             complement(597..2204)
                     /locus_tag="PLM74_03645"
                     /inference="COORDINATES: protein motif:HMM:NF013788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C13 family peptidase"
                     /protein_id="HON42209.1"
                     /translation="MRRVLRYSVILIASLILTGVVAGAPLPVTPQINLQVTPLLRKIV
                     NSALSNLVTMGEVEGQTFGAARNQLVTLDQEGNITRSMSIPIEQPAGISAYTTGRAII
                     GDCGNNTVISVDTRSGQSAKVLDLGATDYGQLVAGDVVRSGRLSSVAFDGKYVYVAMS
                     AGYSSSIFAVDPASNRAVMQAWSPGDKPMAMQFAGGNLYVLDAENSQLRLFDSALRLS
                     TKAIDLQTPDPMGIVIRDNEVRVLTPSDRSIRILKPDLKVLTAPQLEPMWQIKEIVIG
                     PITALARKYALLICGDVAESGYDEFWNDTVWMYKTLLAAGYKENNIYVLYGWGNDYPS
                     ANPNYRHTGKVTDFPATATWVNKVLNGLKNGDSSIGVNKITSIDSLFVWVFDHGGGGN
                     PAYFCLRDGVYYDSDFAGRLNSLPCRERAIFMQQCRSGGFVDNLQNDKSFISAACMWN
                     ENAHRADLEYETYNGVKYHHGEYNYYVISALAGKTIIGAAANADSNGDGKTSAREMHA
                     FMAARENQPEHPQTWDSYGVGAAFLVR"
     gene            complement(2361..3635)
                     /gene="larA"
                     /locus_tag="PLM74_03650"
     CDS             complement(2361..3635)
                     /gene="larA"
                     /locus_tag="PLM74_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HON42210.1"
                     /translation="MRVVLSYGKTGLPVELPDVNVTVVRPVFVPGLDDQRGAVADMLK
                     RPIGCPALREMVRPSDTVAVVFCDATRPVPNRLILPVLLGELESAGVGRDQIVLINAL
                     GTHRPSPPEELVELLGEDLVREYRIVQHDCNDRGTMARAGRLSTGQELWVNKDYLNAD
                     FKILTGFIEPHFFAGFSGGGKLVFPGIASLENIKEAHGYRILSHAKSTWGITHGNPVW
                     ELLTEGAQLTAPDFIVNVTLNSEKAITGVFAGELRAAHARGAEHAKETAMRAVREPFD
                     IVITTNSGYPLDRNVYQTIKGISAAASIVRDGGAIIAAAECIDGIPAGSHFHELLQRA
                     GTPARALELIATPGFSMQDQWQVQLQAEIQTRADVYLYSHRLPKDDIRTTMLKPCDRI
                     EDTLADLLRKYGPGAKICVLPEGPQTIPYVEA"
     gene            complement(3669..4373)
                     /locus_tag="PLM74_03655"
     CDS             complement(3669..4373)
                     /locus_tag="PLM74_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013932409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON42211.1"
                     /translation="MLNVSGVSAGYGGVPALADVSLNIEQGEIVAIVGSNGTGKSTLA
                     RVISGLVKPTSGKITFSGQSIEKMPPHEIVKLGLVHVPEGRHVFGKLTVAENLMLGAY
                     TVSKEADIRARMEFVCGLFPLLKERKNQKAGSLSGGQQQMLALGRGLMANPRLLILDE
                     PSLGLMPKLVEELFESIRNVSREGITILLIEQRVLEALELCDRGYVIQNGRIVLEGPG
                     CELIESDMVRKAYLGM"
     gene            complement(4351..5085)
                     /locus_tag="PLM74_03660"
     CDS             complement(4351..5085)
                     /locus_tag="PLM74_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON42212.1"
                     /translation="MSELVVTGLTKRFGGLLALNGVSFSVRSGEILGLIGPNGAGKTT
                     LFNCLTGFLRIDGGSVEFDGRPITYWSPNRICALGIARTFQVVQVLQGMTVLENVMVG
                     AYLRDGRTAVAQRRAAEVLDRVGMGDKADMIAVNLTLPAKKRLEVAMCLATGPSILML
                     DESMAGLTPTEIHESSAMIRSLRDEGMALVVVEHVMEAIMPIADRVMVLDSGNKIAEG
                     APAEIVRDPRVVSAYLGDKYAERIRC"
     gene            complement(5070..6077)
                     /locus_tag="PLM74_03665"
     CDS             complement(5070..6077)
                     /locus_tag="PLM74_03665"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HON42213.1"
                     /translation="MRDNRQTQIIILIAFTVVGFALPLVAKARFAQHILIMVALYAVL
                     GEAWNIMAGYAGMVSIGQAAFFGLGAYVSSFLFVQYSVNPWIGFLASGLVTAAFGTLI
                     GLPFSRLRGRYFAIGTIALGQMIKVVFENWQRVGAATGLMLPLVDEGVANFQFHTTKL
                     PYYYVAFAMLLAVIAFMRAMERSRMGYYFRAIRENEDVASGLGVNKTAYKLIAIAVSA
                     GITGMCGTFLAQYSLYVEPEYMFNHTISVTIALIPVFGGAGSIAGPILGSAILIPISE
                     LTRAWLGSGGTGIDLMIYGAFIVLMCVYEPEGLMGVVQRANRKAIARRERRGSTQCPN
                     L"
     gene            complement(6077..6937)
                     /locus_tag="PLM74_03670"
     CDS             complement(6077..6937)
                     /locus_tag="PLM74_03670"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HON42214.1"
                     /translation="MILQLATGGLLLGCIYGLVAMGLSLIYGVMGIVNFAHGAFVMLA
                     MYASYWLYSLAGLDPVIGTPLVALLMFGFGIVVYRSIIGKVLRGPFLARLLVTFGLGI
                     FFVNLAQFLWTPDYRMVQESISHGIIMLGPIFIELPKLVASVGSLVVAGLVYWFLRKT
                     KTGLAIQAISIDRTAASLMGIDLERLNAIAFGLGLACAGAAGALLASFYYIFPDVGTM
                     FGLVALVAVALGGFGSTEGALIGAILIGIIETVGGFLVGPEYKYALIFLAYLLIVIVR
                     PKGLMGWGAD"
     gene            complement(7118..8395)
                     /locus_tag="PLM74_03675"
     CDS             complement(7118..8395)
                     /locus_tag="PLM74_03675"
                     /inference="COORDINATES: protein motif:HMM:NF024825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HON42215.1"
                     /translation="MYTRSNRRVAWRIALVAALVCVLAPMMSTMAADNVVRIGAVYPL
                     TGAVATTGADCRTGVELAVDIINGVYDLDFPFAKTAGIPSLGGAKIEMVFGDTRGDPT
                     QGMAETERLITEQKVVAIVGGYQSGVVKTATMASERLQIPYVLSDATSPALTERGFQW
                     FFRVIPHDGIQAKNALELVKDVEKKEKVSIKRVAVLWENTEWGANVAKEIKKWASEYG
                     FTVVADVPYTYRATDVMGEVLKLKAANPEVIIHAAYVSDAILFTQTFKAMKLQPKMII
                     GMAGYLDPNFLNTVGKDGENFFVRAVYGLDLASRKPVVAKINELYKKKYGHDMSPNAA
                     RSFTAPFVVADAINRAKSTKPEDIRKALLETNMPASSLISPYEGVRFDPETHDNVLAR
                     SLYLQVQDGKFRVVWPFELAAVPYVYPFPGWGK"
     gene            complement(8469..9224)
                     /locus_tag="PLM74_03680"
     CDS             complement(8469..9224)
                     /locus_tag="PLM74_03680"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HON42216.1"
                     /translation="MHTERGIRRVKLSDGIVEHIKSLIMAGRLKPGDKLPPEREFAAT
                     LGVSRTALREAVRTLSLMGLLDARQGEGTFVSGLVAGSFMKPLAPMLTMGNIDILELI
                     EARRIIESRAVGLCALRASEDELARISGIADEMQLNIGYIEHFNRLDLDFHMAISSGS
                     HNSVLVATLEAIRDALYEQVQDVQILPGAADRALGYHRRIAESLCIRDSERAERAMVE
                     HLNDVERAVVANIVGKRAIREGRGDNEGESTSV"
     gene            complement(9330..>10529)
                     /locus_tag="PLM74_03685"
     CDS             complement(9330..>10529)
                     /locus_tag="PLM74_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HON42217.1"
                     /translation="PREITGLDVNIVVLLGNGFPSGSHKVGPAYSTLIEACVDGVLDP
                     EKHTILGPSTGNFGIGVSYVCRLMGYRAVVIMPDNMSKERYDRIRKYGAALDLTPGTE
                     SDVILTLERTHELMKDPANFALAQFELLPNYRFHRHVTGSAAVEAALGVGNGRVACFV
                     SAPGSAGTLAAGDHIKSVYPEAKIAAVEPYECSTLSTGGRGQHRIEGIGDKMCTLIHN
                     VLTTDFIITVHDDECVQALKVMREGAHILERLGVKREAADSMRDLFGVSGMCNVIAAV
                     RMARYLRLGPDENVVTVATDGFDRYESVIDDLDRRYLETTDHVLERWACDIFAAQDDA
                     AIHDVRRAEAKEKLYAQKERDWTPFGYSVEYLDSMKSQDFWEDEYAKYAYYNEKIGQM
                     RGASGWR"
BASE COUNT         2204 a         3238 c         2770 g         2317 t
ORIGIN      
        1 acactgagca gctatgcaat cgcgggggat cgagcttcag cgtcgccggc ggttcgagca
       61 ggattcccgg cgactaggga caggggcaag ttggggctgc gttcactgta agtcctatct
      121 cgatgttgct tatcttgtcc aggcgtatca gtgtgcactg cgcgtactcg gtgttaacta
      181 ctcctcctcc gcaaaacaac ttgatgatta tccccgggca cgggttgaag gccgccagag
      241 ccaggaatcc accacagata ccggtgatcc tagcgttgca cgccttctta cagcaatgac
      301 cgcattcgaa ctccacagcc acaggctggc acggatctgg gcactgagct atgaaggccc
      361 cgatagggtg cctgttgagc gcctccgcgg agaacatctc ggcctcctgg gagattgggg
      421 tatccattat ctgccgccac ctttcagcgt tcttggcccc ttccgggcca tactaccatc
      481 aaggtatgct cctgcggcag agtatcaccc ctgcgtattg tcacgattta tacgattgac
      541 gtagaaaggc cgggctcccc ttcacgggtt gcccggcctc cttcgcgctt atccgactac
      601 ctaaccagaa atgccgcacc tactccgtag gaatcccacg tctggggatg ctccggctgg
      661 ttctctcgcg ccgccatgaa ggcatgcatc tcccgagcgc ttgtcttgcc atcgccgttg
      721 gaatcggcgt tggctgctgc cccgatgatt gtcttgccgg ccaaggcgct gattacgtag
      781 tagttgtact caccgtgatg gtatttcaca ccgttgtacg tctcgtattc gaggtctgcc
      841 ctgtgcgcgt tctcgttcca catgcaggca gcggagatga agctcttgtc gttctgcagg
      901 ttgtctacaa agccgccgct tctgcactgc tgcatgaaga tggccctctc tctgcatggc
      961 agcgagttca gcctgccggc gaaatccgaa tcgtagtaga ccccgtcccg caggcagaag
     1021 taggctgggt tgccgccgcc tccatggtcg aacacccata cgaagaggct atcgatggag
     1081 gtgatcttgt tcacgcctat cgacgaatcg ccattcttga gcccatttag gaccttgttc
     1141 acccaggtcg cagtagctgg gaagtcggta accttgccgg tgtgccggta gttggggtta
     1201 gctgaggggt agtcattgcc ccagccgtac agcacgtata tgttgttctc cttgtagcct
     1261 gctgcaagca gtgtcttgta catccatacc gtgtcgttcc agaactcatc gtacccgctt
     1321 tccgccacat ccccgcatat gagcagggcg tatttcctag caagcgccgt gatggggccg
     1381 atcacgatct ccttgatctg ccacataggc tctagctggg gcgcagtcag caccttgagg
     1441 tcaggcttga ggatccttat gctcctgtca gaaggtgtta gtacccttac ttcgttgtcc
     1501 ctgatgacta ttcccatggg gtccggagtc tggaggtcta ttgctttggt gctcagcctg
     1561 agggccgaat caaacaaccg cagttggcta ttctcggcat ccagaacata gagattgccc
     1621 ccggcaaact gcatggccat aggcttatct cccggcgacc atgcctgcat gaccgcccta
     1681 ttcgacgccg gatccacggc aaagatcgag cttgaatagc ccgccgacat cgcaacatat
     1741 acatacttac catcgaaagc aacgctcgat agtctaccgc ttctgaccac atcgcccgcc
     1801 acaagctgcc cgtagtccgt tgccccaaga tccagcacct tggcagattg accggacctt
     1861 gtgtccactg aaatcacggt gttgttgccg caatcgccaa ttatcgccct gcccgtcgta
     1921 tacgcggata ttcccgcggg ttgctcgatc ggtatcgaca tcgaccttgt gatgttaccc
     1981 tcttgatcca gcgtgaccag ctgattcctt gcggccccga aggtttgacc ctcgacctca
     2041 cccatggtga ccaggttcga cagcgcagaa ttcacgatct tgcgtagcag aggtgttacc
     2101 tgtaggttga tctggggagt gaccggaagc ggagccccag caacgactcc ggtcagaatc
     2161 aacgatgcta tcagtatcac agaataccta aggacccgac gcatctctct cactccttct
     2221 tctgcgtatg tatatgcagc ggagcagaat ttcgccaaga gccggattat tcctgtatcg
     2281 gggaaaaata aaccgccgtc tccggcggtg tgtagacgtc gtcatcggta tggccaacga
     2341 ttacaggcaa tgaaatggta tcaggcctcc acgtatggta tggtctgagg gccttcgggc
     2401 agcacgcata tcttagcgcc cggaccatac ttgcgcagca agtccgccaa tgtatcttcg
     2461 atcctatcgc atggcttgag catagtggtg cgtatatcat ccttcggaag acgatgggag
     2521 tacaagtaga catctgccct tgtctggatc tcggcctgta gctgtacctg ccattggtcc
     2581 tgcatggaga agcccggggt tgcgatcagt tccagcgccc gcgcgggcgt gcctgccctc
     2641 tgcagcagtt cgtggaaatg gctccccgca ggtatgccat cgatgcactc tgccgcagcg
     2701 attatcgccc ctccatccct tacgatggat gccgctgcgg agatgccttt tatggtctga
     2761 tacacatttc ggtccagtgg atacccgcta ttagtggtga tcacgatgtc gaaaggctcc
     2821 cgcaccgccc tcatagccgt ctccttagcg tgctccgcgc ctcgcgcgtg cgccgcccgc
     2881 agttctccgg cgaatacgcc ggtgatcgcc ttctcagagt tcagcgtaac gttgactatg
     2941 aagtccggtg ccgttagctg cgcgccctca gtaagcagtt cccacacagg attgccgtgc
     3001 gtgatgcccc aggtcgactt ggcatgcgac agtattcggt atccgtgggc ttccttgatg
     3061 ttctcaaggc tcgcgatacc tggaaacacg agcttacctc cgccgctgaa cccagcaaag
     3121 aagtgaggtt ctatgaagcc ggttagtatc ttgaaatccg cgttgaggta atccttgttc
     3181 acccacaatt cctgcccggt gctgagacga ccggcacgcg ccatcgtccc gcggtcgttg
     3241 cagtcgtgct ggacgattcg gtattccctg accaggtcct cgccgagaag ttccacgagt
     3301 tcctcaggcg ggctgggcct gtgcgtgcca agcgcattga tcaggactat ctggtccctc
     3361 cctacgccgg cgctctccag ctctcccagg agaaccggga ggataagcct attaggcaca
     3421 ggcctggtcg catcacagaa gaccaccgcc accgtatccg aaggcctaac catctcgcgt
     3481 agcgccgggc accctattgg acgcttcaac atatcggcga ctgctccccg ttggtcatcg
     3541 agccccggta cgaacactgg ccggactaca gtcacattga cgtctggcag ttctacgggc
     3601 aatcccgtct tgccgtaaga tagtacaact ctcactacat gactcccccg tcgatggtca
     3661 gtatggcgtc acatccccag ataggccttg cggaccatgt cgctctctat cagctcgcac
     3721 cccggcccct ccagtacgat cctgccgttt tggataacgt accctctatc gcacagttcg
     3781 agggcctcca gcactctctg ttcgatgagg agtatggtta tcccttccct gcttacgttg
     3841 cgtatggact cgaaaagctc ctcaaccagt ttgggcatga ggccaagtga gggttcgtcc
     3901 agtatgagga gtcggggatt ggccatcaaa cctctaccca gcgccagcat ctgttgctga
     3961 cctccgctga ggctgccggc cttctggttc ttccgctcct tgagcaacgg aaacaggccg
     4021 catacgaact ccatccgcgc gcggatatct gcctccttag agacagtata cgcacccagc
     4081 atgaggttct ctgcgaccgt caatttgccg aagacgtgtc ttccctcggg cacgtgcacc
     4141 agtccgagtt tgactatctc atggggcggc attttctcta tactctggcc gctgaatgtt
     4201 atctttccgg atgtcggctt caccaggccg gatatcacac gtgcaagagt ggacttgccg
     4261 gttccgttcg acccgacaat ggccacgatc tctccttgct caatgttcag cgatacatcg
     4321 gcaagcgccg gcaccccacc gtaacccgca ctaacacctg atacgttcag catatttatc
     4381 acctaagtac gccgatacca cccgcgggtc tctcacgatt tcggcaggtg cgccctcggc
     4441 gatcttgttt cctgaatcca aaaccataac ccgatcagcg atgggcatta tggcttccat
     4501 gacgtgttcg actactacaa gcgccatgcc ttcatcacgc agactccgga tcattgccga
     4561 cgactcatgt atctctgtag gggtcaggcc ggccatggat tcatcgagca tgaggatgct
     4621 cgggcctgtg gcaaggcaca tagccacttc cagacgcttc ttggcaggta gggtcagatt
     4681 gactgcaatc atgtccgcct tgtcgcccat gccgacccgg tcgagcactt cagccgcccg
     4741 tcgctgagct actgccgtgc gtccgtctct caagtaagcg ccgaccatca cattttccag
     4801 tactgtcatg ccctgcaata cctggactac ctgaaaagtg cgtgcgatac ccagggcgca
     4861 aattcggttg gggctccagt acgtgatcgg gcgcccatca aactccaccg aacccccgtc
     4921 tattctgaga aacccggtca agcagttgaa caaggtggtc ttgccggcgc cgttggggcc
     4981 gatcaacccc agtatctctc cgctcctgac ggagaacgaa acaccattca gagccagtag
     5041 ccctccaaat cgcttggtga ggcctgtcac tacaagttcg gacattgagt cgaacccctc
     5101 ctctcgcgcc gcgctatcgc ctttctgttt gcccgctgca ctacgcccat gagcccctcc
     5161 ggctcatata cgcacatcag cactatgaac gcgccatata tcatcaggtc tattcctgtt
     5221 ccgcctgatc cgagccacgc acgcgtcaac tccgatatgg ggatcagaat cgcagatccg
     5281 agtatgggcc ctgctatgct gccggcccct ccgaacacag gaatcaaggc gatagttacc
     5341 gagatcgtgt ggttgaacat gtactcgggc tcgacataca gactgtactg cgcaaggaag
     5401 gtaccgcaca tccccgtgat gccggcgctc actgcgatcg caatgagctt gtatgcagtc
     5461 ttgtttaccc ccaggccgct ggcgacgtct tcattctcac gaattgctct gaagtagtag
     5521 cccattctgg atcgctccat ggcccgcatg aaggctatca cggcgagcag cattgcgaag
     5581 gcaacatagt agtaaggaag cttggtagta tggaactgaa agttcgcgac gccctcgtcc
     5641 accagcggca gcatcaggcc tgtggctgcg ccgactcgtt gccagttctc gaacacgacc
     5701 tttatcatct gacccagtgc gatggtgccg atcgcgaagt accttccgcg cagacgcgag
     5761 aatggcagcc caatcagtgt tccgaaggcc gcggtcacga gccctgaggc tagaaagcca
     5821 atccacgggt tcacactgta ctgtacgaac aggaatgagg atacgtaggc gcccagaccg
     5881 aaaaacgcag cctgacctat cgacaccata cctgcgtacc cagccattat attccacgcc
     5941 tcgcctagta cagcatacag cgcaaccatg atgagtatgt gctgcgcgaa gcgggctttc
     6001 gccacaagcg gcagcgcgaa acccacaacc gtgaaggcta tgagtatgat tatctgtgtc
     6061 tgacgattgt cgcgcattag tcggcccccc atcccatgag cccttttggc cgtacgatga
     6121 ctatcagcag gtaggccagg aatatgagcg catacttgta ttcaggccca acgaggaaac
     6181 cgcccacggt ctcgataatg ccgatgagga tcgcaccgat aagcgcgccc tctgtgctgc
     6241 cgaaaccacc gagcgctact gccaccaaag ctacgaggcc gaacatcgtg cccacatcgg
     6301 gaaagatata gtagaagctt gcaagcagcg ctccggccgc acctgcgcag gccaggccaa
     6361 gcccaaacgc tatagcattc agtcgttcga ggtcgatacc catgagcgag gcagcagtac
     6421 ggtcgataga tatggcctgg atcgcaaggc cggttttggt tttgcggagg aaccagtaga
     6481 ccaaccccgc caccaccagg ctgccaacgc tcgctaccag tttgggtagt tcaatgaaga
     6541 tcggccccag catgattatc ccgtgggaaa tggactcctg aaccatgcgg tagtccgggg
     6601 tccacagaaa ctgggccagg ttgacgaaga agataccaag tccgaacgtg accaaaagac
     6661 gggcgaggaa cgggccacgc aagacttttc cgataattga acgatatacc acgatgccaa
     6721 acccgaacat cagcaaagca actagaggcg tgccgatgac ggggtctagg cccgccaggc
     6781 tgtacaacca gtaacttgca tacatggcca acatcacaaa agccccatgg gcgaagttga
     6841 cgattcccat gactccatag atgaggctca aacccatggc aaccagaccg tatatgcagc
     6901 cgagcaacag gccccccgtt gcaagttgga gtatcacgga agaccacccc tgtcaaagtg
     6961 tatggcagac aagaacaagt agatgactga gaatgtcact gcggtagtcc cactgacgaa
     7021 aggcgcccga gcaggcgggg aaggcatgcc gagagtcgct gtaaaccgcc ccacgcctgc
     7081 ccggttcctg tgctatgtca gccagactat gtctgcttta cttaccccat cctgggaacg
     7141 ggtatacgta aggcacagcc gccagctcga acggccatac taccctaaac ttgccgtcct
     7201 gcacctgaag gtagagagac ctcgcaagca cgttatcgtg agtctcaggg tcgaatctga
     7261 ctccctcgta aggcgaaatg aggctgcttg ccggcatatt ggtctcaagc agcgccttcc
     7321 tgatatcctc gggcttggtt gatttggccc ggttgatcgc gtctgccacg acaaacggag
     7381 cggtgaagct gcgagccgca ttgggactca tatcgtggcc gtacttcttc ttgtatagct
     7441 cgttgatctt ggccacgaca ggcttgcgcg aggccaggtc aaggccgtac actgcgcgaa
     7501 cgaagaagtt ctccccgtct ttgcccacgg tgttcaggaa gttgggatca aggtatcctg
     7561 ccattccgat gatcatcttc ggctgcagct tcatggcctt gaacgtctgc gtgaacagga
     7621 tggcgtcaga cacatacgca gcgtggatga tgacctcggg gttggcagcc tttagcttga
     7681 gcacctcgcc cataacatca gtagcccggt atgtgtaagg tacgtctgca acaacagtaa
     7741 atccgtattc actggcccac ttctttatct ccttggctac gttggcgccc cattctgtgt
     7801 tctcccagag tacggccact cgcttgatgg agaccttctc tttcttctcg acatccttca
     7861 cgagttcaag agcattcttg gcctggatgc catcgtgcgg gataacccgg aagaaccact
     7921 gaaacccacg ctcagtgagt gctggggagg tggcgtccga caggacgtac ggtatctgaa
     7981 gtcgctcgga tgccatggta gctgtcttca caacgccact ctgataaccg ccgacgattg
     8041 cgacgacttt ctgctcagtg atcaggcgtt cagtctctgc catgccctgt gtcggatctc
     8101 cacgagtatc tccaaatacc atttcgatct tcgccccgcc caggctcgga atgccggcgg
     8161 tcttggcaaa cggaaagtcc aaatcgtaga cgccgtttat gatgtctacg gccagttcga
     8221 cgccggtacg gcagtcggcg cccgtggtgg cgactgctcc ggtgagagga tagacagcgc
     8281 cgatcctgac gacgttatca gccgccatcg tgctcatcat cggcgcaagc acgcacacca
     8341 gtgcggcaac taacgcgatc ctccaggcaa ccctacgatt actgcgtgtg tacaccgtca
     8401 tgcctcccta agttgatttc ggccgctgga tctaccagcc atacttgccc ccggacaact
     8461 gagacgccct acacagatgt gctctctccc tcattatcac ctcttccctc gcgaattgcg
     8521 cgtttcccaa ctatgttggc cacgaccgcc cgctcgacgt cgttgaggtg ctcaaccatc
     8581 gctctctccg cacgctcact gtcgcgaatg cagagagatt ccgcgatgcg gcgatgatac
     8641 ccgagtgctc tgtcagcggc gccgggaaga atctgaacat cctggacctg ttcgtagagg
     8701 gcatctcgaa tggcttccag ggtcgccact aacaccgaat tgtgagaacc gctcgatatg
     8761 gccatgtgaa agtcaaggtc gagtcggttg aagtgctcta tgtacccgat gttgagctgc
     8821 atttcgtcgg caatgccgga gatgcgcgcc agttcatctt cggaggctct cagggcgcaa
     8881 agacccaccg ccctagattc aattattcgc ctcgcctcga ttagctccaa gatgtcgatg
     8941 tttcccattg tcagcatggg ggcgagcggt ttcatgaatg agcccgcaac caaccctgac
     9001 acaaaagtac cctcgccctg cctcgcatca aggaggccca taaggctaag ggtacggact
     9061 gcttcacgta gagctgtgcg actcactccc agagtcgccg cgaactcccg ttccggtggc
     9121 agcttgtcgc cgggtttcag gcgccctgcc atgataagcg atttgatgtg ttcaactata
     9181 ccatctgaaa gcttgaccct gcggataccc ctctcagtat gcacaaggga cacctccaat
     9241 ggtatgagga tcataccact gtagatatac gacgcccgga ggacaattcc tcctgggcgt
     9301 cgtacaggtg cgtagtcaca taatcggcat cagcgccatc cagaagctcc gcgcatctga
     9361 cctatctttt cgttgtagta tgcgtacttg gcatactcgt cttcccagaa gtcctgcgac
     9421 ttcatgctat ccaagtactc cacgctgtag ccgaacgggg tccagtccct ctccttctgc
     9481 gcatacagct tctccttggc ctccgccctt ctcacatcgt gtatcgcggc atcatcctgt
     9541 gcggcgaata tgtcgcacgc ccacctctcg agcacgtggt cggtggtctc aaggtaccga
     9601 cggtccagat catcgatgac cgattcgtac ctgtcgaacc cgtctgttgc gactgtgaca
     9661 acattctcgt cggggcccag cctgagatag cgagccatgc ggactgcggc tatgacattg
     9721 cacatgcccg acacgccgaa caagtcgcgc atagaatccg ccgcctcgcg cttcaccccc
     9781 aaccgctcaa gaatgtgcgc gccttcgcgc atcaccttca gagcctgaac gcactcatcg
     9841 tcgtggacgg tgatgatgaa gtccgtggtc agaacattgt ggatgagggt gcacatcttg
     9901 tcgccgatac cctcgatccg gtgctggccg cgaccgcccg tcgacagcgt cgaacactcg
     9961 tagggctcca ctgccgcaat cttggcctcc ggatagacgg atttgatgtg atctcctgca
    10021 gccagcgttc ccgccgaccc aggcgccgac acgaagcatg ccacccgtcc attaccgacc
    10081 ccaagcgccg cctccaccgc cgccgacccg gtcacatgcc tgtggaacct gtagttcggc
    10141 agcagttcga actgggccag agcgaagttt gcggggtcct tcatgagctc gtgtgtgcgc
    10201 tcaagcgtca ggatcacgtc cgactcagtg cccggtgtca ggtcgagtgc ggcgccgtat
    10261 ttgcggatgc gatcgtatct ttccttgctc atgttgtcgg gcatgatgac taccgcccga
    10321 taccccatga gccgacagac gtacgagacg ccgattccga agttgcccgt agacggcccc
    10381 agaatcgtgt gcttctccgg atcgagcacg ccgtcgacgc acgcctcgat gagcgtagag
    10441 taggccgggc ctaccttgtg cgatcccgac ggaaacccgt tccctaggag aaccacgatg
    10501 ttgacgtcga gtccggtgat ctcccgcgg
//
LOCUS       DAOTAC010000054        10440 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_5430_length_10440_cov_4.633028, whole genome shotgun sequence.
ACCESSION   DAOTAC010000054 DAOTAC010000000
VERSION     DAOTAC010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 10440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10440
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5430_length_10440_cov_4.633028"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="PLM74_03690"
     CDS             complement(<1..325)
                     /locus_tag="PLM74_03690"
                     /inference="COORDINATES: protein motif:HMM:NF024485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HON42218.1"
                     /translation="MANEVQTITVKVRRADPDVNGGEASYQSYTVPLEPGSTVLSVLR
                     YITENIDRTLSYYRSCRIGKCAGCRMVVNGKTRLACTTAVTGDITLDPLPGYPLIKDL
                     VVDLSA"
     gene            complement(332..2101)
                     /locus_tag="PLM74_03695"
     CDS             complement(332..2101)
                     /locus_tag="PLM74_03695"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HON42219.1"
                     /translation="MSTLEKTADEIVITDVLIVGSEGAGARAALEASKRCKSVVVATK
                     GVVGKSGATLTADADIDIDSRSAAELFGLPGDMNDSPEKFAEDMCKEGEYLNNQRLVK
                     IHCDEAPMRLKELVDWGARIDKLTHAPGHTYPRGVWVPGTEFARVLTKELKKRDNIKL
                     LEHMMITDLLTDNGRVVGAAGIEITTGKFFVIKAKAVIFCTGGAMRIYPHTTAPDELS
                     GDGLAMAYRVGAKLIDMEFPMFLPYTLIKPDSLNGVDFSYLLSAYVQAQALNRHGERY
                     MAKYDPERLEHSTRDVNSVAAMIEVLDGKGSPAGGTYLSLKHLPDNLIDFSAEWFPSN
                     ISHWRYGGFNMTDYLPDLHTQAMETSPASHFWNGGVQIDEFCQTGIAGFYACGEGTGS
                     IHGANRVSGNALTMTQVWGPRAGIYASEYANKAGSGNIDLDQVMAIKERLHAPLKRDD
                     GVDPIALRKQIQDVAWKYVGVVREEKGLNTGLEEIAAMRAQLDQVYVSNKAKRFNHEW
                     VEALQLENMLDVMEMVAEASLVRKESRGALYRRDFPKTDNINWVKNVIVQKDGDKIAT
                     SVHGVDLEKYQPTREIRDYGSKE"
     gene            complement(2126..3286)
                     /locus_tag="PLM74_03700"
     CDS             complement(2126..3286)
                     /locus_tag="PLM74_03700"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HON42220.1"
                     /translation="MRGQYSIFPREEYEARLGRAQELMQAQNIDALLITAEANYFYFT
                     GHRTHSPWSTFTRPHVAVLTRDGGATMIVHCFTRPEAQSRSFVSDVREYGSLMEDAVP
                     QIKQALSDMGLASAVIGCELGYEQRLGLPMLAWKQIVSAFPRAKFVDASELLWRLRVV
                     KSEAEVERLRKACQATSRAFEACYSQVREGWTEEQVAVVMSKAMLDAGAEAPGFNIIC
                     SGKDDYARISARPTGRALRKGDMLWVDASAVFDGYWSDFCRAAVPGGPNPEQVRLQRI
                     AHEATMAAVEAIKPGVPVKMIAETCARQLGKHGFNLTFDAGRCGHGLGLASTEPPHVA
                     TYDPTILEEGMVITVEPGIVNEEGVFCIEENVAVTKDGFEVLSGASREIYAI"
     gene            3674..5455
                     /locus_tag="PLM74_03705"
     CDS             3674..5455
                     /locus_tag="PLM74_03705"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HON42221.1"
                     /translation="MLSLRMVQGDVQRVAEAIAVALDVDVEITDRDLIRVAGTGEFSR
                     QLGNSLAGQGRVHAHVLQTGATVVIENPGDHELCRTCVMAKRCYATAELCCPINHGDA
                     VAGVVGLVSHTQAQRRRLLDNVRANVAFLERMADLLSAKITSEEAMVRDRFRRRQLEA
                     VIGAFDQGVLVVDEYGVMTQANRYALDALHLSPEVIVGKPLQDVLTGTGLYDTLGSGI
                     EIKHHRTRLKQHSGDTIDAICNAYPVAVDGKTLGAVLTFQRLDDVSRFTYEIWEHENR
                     ATSFDDIIGDSRPMSSVKEFAAKIAPGNSTVLLRGESGTGKGLMARAIHRASRRKDGP
                     FVVVNCSAIPDTLLESELFGYEEGAFTGARKKGKLGRFELANNGTLFLDEIGDMPLHL
                     QGKLLRALEDRMIEPVGSVESRPIDVRIIAATNRDLDAMVESREFRKDLFYRLNVVPI
                     WMPPLRKRREDIPMLARFFLDKNCRTAGKSTVAFSDEALRCMLAYEWPGNVRELSNSV
                     EYAVNIESESTVSIASLPQRIRTAPGPGSTDNADGPQALEALERQAIVEALQRFDGEP
                     RAKENAARELGIHVTTLYRKIAKYNLA"
     gene            complement(5459..7348)
                     /locus_tag="PLM74_03710"
     CDS             complement(5459..7348)
                     /locus_tag="PLM74_03710"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HON42222.1"
                     /translation="MNGGLAMLGGYMGRVLWVDLSTGKLTDQRLDDDICQDYIGGYGI
                     GMRMIYDNQQAGLDPLAPESIVAFGTGPLTGSPAVIGSRFTVMGKSPVSGTWGDANSG
                     GYFGPTLKRAGYDMVFFRGQSETPVYLVIDNGRAELRDASGLWGMTTYDVDSTLKGLF
                     GRDAQVACIGPAGERGSLLSCIIHDRGRAAARSGFGAVLGSKKVKAIVVRGDKPVPVA
                     DSERAKALRSKYVKQIRVDHVGSSQLYMVGTPALFGLCALGGDSPVKNWAGASLHDFT
                     QSQIEALHHDRFYKFKTKKYGCFGCPIACSGTFDIEEGPYAVHDSHQPEYETIAAFGS
                     NCMNSSPESIIKANDICNSYGLDTIGTGSMIAFAMEAYEKGLISKEDAGGLELSWGNP
                     DAIVKLAEDIALGRGLGEVLSMGLERAAKVIGGGAESFAIHVQGEAIAMHDPRFEPTM
                     SVIYRMFPGKHIQASQFCKPPAFDDEIPAYGSDRERQAGRGTQLKSLECLCNVVNCAG
                     VCVFGYLSTHVDCVPEFLSAVMGVDMGVPEAIEAGERISNMRQAFNIREGVNLIKAHV
                     PGRMLGNPPQQEGPTAGFSVDSGLILSEYLDAMQWSHEDGRPSKRRLLELGMEDIAQD
                     LYVAS"
     gene            complement(7402..8226)
                     /locus_tag="PLM74_03715"
     CDS             complement(7402..8226)
                     /locus_tag="PLM74_03715"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HON42223.1"
                     /translation="MNGPKGKIAVLVVAIIVVFALNFPVYWMFVQSITSESMFTERPS
                     LLPKAPSLDAFRRICEAKPIGQWFVNTLMVAVGTVLLSMPLAAGAAFSLSRYKTTLNS
                     ITGLAILGTQMLSASLLAIPIYVLFRRTGLHDKLGGLIVANTAFTLPMSIWVLKGFFD
                     AIPKELDEAAMIDGCTVGQMIRRVIMPLVLPGIVAVSIFAFILAWDEFFFARTLVSSQ
                     GNWVMSVGLSSFREEFTIQWSDMMAAAVLFTIPPIVLFMFIQKHLVSGLTGGAVKG"
     gene            complement(8234..9112)
                     /locus_tag="PLM74_03720"
     CDS             complement(8234..9112)
                     /locus_tag="PLM74_03720"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HON42224.1"
                     /translation="MRTASPNAAAAGNKASSALILPAAMVMGAVVIYPILTTMWLSLT
                     DAPLISLSPPSFAGLSNYADWLSNPEFWRSLWITLVYTFGVTVGSYAIGLITALLLNM
                     EFPGRAILRGLVLLPWAIPEVAAVMTWNWMLDYQFGIVNSLLGSQIGWVTDSAVAPWT
                     VTIVTIWKQFPLATVVLLAGLQAIPRELYEAANVDGASKLQQFIYVTLPGLKPVNSVL
                     VMMLILYTFKRISIIYLLTGGGPARATEILPVTTYLEAFKYFRLGSASAVGILALLFT
                     LVITLVYSRLFMREEA"
     gene            complement(9214..>10440)
                     /locus_tag="PLM74_03725"
     CDS             complement(9214..>10440)
                     /locus_tag="PLM74_03725"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HON42225.1"
                     /translation="LIALVMAPIGIASGANKQIQFPSWMWGEAGTGDWFKDGVALYES
                     QNPGVKVAPALVPAGQYEEKLLIDMAGGGAPDLAPVFTNMMPKLMRMGLLEPLDDYLA
                     KAEWKDNLLPVQKVAQYKGKTYGVVLTASPNGLIYNKQLLDKAGVGVPTTPEEMLEAA
                     RKVKKATGAFGYGFATRTADVLEAYIPLMQWAIGFGGDFSKDGVPTANDPKTIEGLNF
                     LKRFFDEDLTPKGLDGPMLRKMFVEGKIAMLIDGPWAITYVKEAAPDLYKYVGFAAPP
                     TPTHAAITGGAFYTIPVRAKYKQEAFALLSVYNQPEWQSRWLEDLLQIPGQKAEISPK
                     LAKEHPWIEGMVDVAAKYPAGFGYSAPGFEEYAGEVQRMVVDGIARVWGGRATVEAAM
                     NDVQQKMVKWAATLKR"
BASE COUNT         2174 a         3191 c         2823 g         2252 t
ORIGIN      
        1 cagcactgag gtccacaacc aggtccttga tgagagggta acctgggagc ggatcgagcg
       61 tgatgtcgcc cgtgaccgca gtcgtgcatg caagtctggt cttgccgttg acgaccattc
      121 tgcatccagc gcacttgcct atcctgcacg accggtagta cgacagcgtc ctgtcgatgt
      181 tctcagtgat gtacctcagc acgctgagca cggttgaacc cggctccagc ggcacagtat
      241 acgactgata gctagcttcg ccgccgttga cgtcagggtc tgcccgccgc accttgacag
      301 tgattgtctg tacttcgttc gccatccgtt gtcactcctt gctcccgtaa tcccggattt
      361 ccctggtcgg ctggtatttc tcgaggtcga cgccatgcac cgacgtggca atcttatccc
      421 cgtccttctg cacaatgacg ttcttgaccc agttgatgtt gtcggtcttg gggaagtctc
      481 tgcggtagag agcgccccgg ctttccttcc tcactaggct tgcctcggcg accatctcca
      541 tgacgtccag catgttctca agctgcagcg cctccaccca ttcgtgattg aagcgcttgg
      601 ccttgttgct cacgtacacc tggtcaagct gggcgcgcat cgctgcgatc tcctcaaggc
      661 ccgtgttgag ccccttctcc tcgcggacca cgccgacgta tttccacgcc acatcctgga
      721 tctgcttgcg cagagcgatc gggtcaactc catcgtcgcg cttcagaggc gcatggagcc
      781 gttccttgat ggccatgacc tgatcgagat cgatgttgcc gctgccggcc ttgttggcat
      841 actcggaagc gtagattccg gcgcgcggtc cccacacctg ggtcatggta agggcgtttc
      901 cggatactct gttcgcgccg tggatgctgc cggtaccctc gccgcaggcg tagaagccgg
      961 caataccggt ctggcagaac tcgtctatct gaactccgcc gttccagaag tggcttgcag
     1021 gcgaagtctc catggcctgg gtatgcaggt caggcaggta gtccgtcatg ttgaacccgc
     1081 cgtagcgcca gtgcgatatg ttagacggga accattcggc gctgaagtcg atcaagttgt
     1141 cgggcaggtg cttcagggag aggtaggtgc cgcccgcagg cgaacccttc ccgtcaagca
     1201 cctctatcat cgcagcgacg gagttgacgt ccctggtgct gtgctcaagc ctttcagggt
     1261 cgtacttagc catgtatctc tcaccgtggc gattcaaggc ctgtgcctgg acataagcgc
     1321 tcagaagata tgagaagtcg acgccgttca gcgaatccgg cttgatcagg gtgtacggca
     1381 ggaacatggg aaactccatg tctatgagct tggcgcctac ccggtaagcc atcgccagtc
     1441 cgtcgccgct cagttcgtca ggagcggtag tgtgcggata gatgcgcatg gcgccgccgg
     1501 tgcagaatat aactgccttt gccttgatca cgaagaactt gccggtggtt atctcgattc
     1561 cagctgcgcc caccacgcgt ccgttgtcgg tgagcaggtc agtgatcatc atgtgctcga
     1621 ggagcttgat attgtctctc ttctttagct ctttcgtgag cacgcgcgcg aactccgtgc
     1681 ctggaaccca tacgcccctg gggtaagtgt gaccgggcgc atgtgtcagc ttgtcaatgc
     1741 gggcgcccca gtcaaccagt tccttcagcc tcatgggcgc ctcgtcgcag tgaatcttga
     1801 ccaggcgctg gttgttgagg tactctccct ctttgcacat gtcttcggcg aatttctcgg
     1861 ggctgtcgtt catgtccccg ggcagtccga acagctctgc ggcgctgcgg ctatcgatgt
     1921 cgatgtctgc gtcggctgtg agcgtggctc cgctcttgcc cacgactccc ttggttgcta
     1981 ctaccaccga cttgcaccgc ttgctcgcct ccagcgctgc cctggctccc gcgccttcgc
     2041 tgccgactat cagtacgtca gttattacga tctcatcagc ggtcttttcg agtgtactca
     2101 ttctccacct ctccatcctg gcaaactaaa tggcgtagat ctcccgcgaa gctcccgaga
     2161 gaacctcaaa tccgtccttg gtcactgcga cgttttcctc tatacagaac actccttcct
     2221 cattcacgat cccgggctcc acggtaataa ccatgccttc ttccagaatc gtcggatcgt
     2281 atgtggcgac gtgggggggt tcagtgctcg caaggccgag cccgtggcca caccgaccgg
     2341 cgtcgaaggt aaggttgaaa ccatgcttgc ctagctgcct ggcgcacgtt tcggcaatca
     2401 tcttgacagg cactcctggc tttatcgctt ctacagcagc catcgtggcc tcgtgcgcta
     2461 tccgctggag cctaacttgc tcagggttgg gtcccccggg aaccgccgcc cggcaaaagt
     2521 cgctccaata gccgtcgaac accgcactgg cgtctaccca gagcatgtcg cccttccgca
     2581 gagcacgccc tgttggacga gcgctgatcc ttgcgtaatc gtccttgccg gaacagatga
     2641 tattgaaccc gggggcctcg gcgcctgcgt ccagcatcgc cttgctcatc acgactgcaa
     2701 cctgctcttc tgtccatccc tcgcgcactt gtgaatagca ggcttcgaac gccctgctag
     2761 tcgcctgaca agcttttctc aagcgttcca cttccgcctc ggacttcacg actcgcaatc
     2821 tccaaagtag ctcggatgcg tccacgaact tcgccctggg aaaggcggat acgatctgtt
     2881 tccacgccag catgggcagc ccaagcctct gttcataccc aagttcgcat ccgatgacgg
     2941 ccgaggccag ccccatgtcc gacaacgcct gcttgatctg gggcaccgca tcctccatca
     3001 aactgccgta ttcgcggaca tcggacacaa atgaccgtga ctgcgcctct ggcctggtaa
     3061 agcagtgcac gatcatggtg gcccccccgt cgcgcgtgag aacggccacg tgtggacggg
     3121 tgaaagtcga ccacggcgag tgagtcctgt gacccgtgaa gtagaagtag ttggcttctg
     3181 ccgtgatcag cagggcgtcg atattctgcg cctgcatgag ctcctgggcc cgtcctaacc
     3241 ttgcctcgta ctcctctctg gggaatatgg aatactgacc tctcatccct atctaccagt
     3301 ccttcctcgt gctgtgtgtt ttcgccggtc ctaccgtcca ctatgcgttg agatcgcaga
     3361 caacctatcc gtcacagaga taaatgcaag atacgtgcca ccgcaggttt ccacaaaacg
     3421 cggcggaggt cccgagagcg gtcacgcccc cgaatgccgc tgtgacctgg gctatcgcca
     3481 atcatcacat ccctgtgcgt ctcacaatcg gcgtcgccgc agccatttat gcactacgta
     3541 gtagcgcatt gcagtagcaa attgcaatac cgtcttgcaa tttgctatta tgaagacttc
     3601 tatgctatgc tcacatggaa gggcccgctt cgggccccat ctgcgccgtc tgtagtcagg
     3661 ggaggatgcc cacgtgctaa gcctgagaat ggttcagggt gatgtacaga gagtagccga
     3721 agccattgca gtagctctcg atgttgacgt ggagatcact gatcgggatc tgatcagggt
     3781 ggccggcaca ggggagttca gcaggcaact gggaaacagc ctcgcagggc agggacgggt
     3841 ccacgcccat gtgctgcaga ccggtgccac tgtagtgatt gagaatccgg gtgatcatga
     3901 gctatgccga acttgcgtga tggcaaagcg ctgttacgcc acggccgaac tatgttgccc
     3961 catcaaccat ggagacgcag tcgccggagt agtggggttg gtcagccaca cgcaggcgca
     4021 acgccgaagg ttgctcgaca atgtgcgtgc caatgtcgcg ttcctggaac gcatggccga
     4081 cctgctcagc gccaagataa ccagtgaaga agccatggtg cgcgatagat tccgccgccg
     4141 tcaactcgaa gcggtgatag gcgcgttcga tcagggcgtg ctggtggtgg acgaatacgg
     4201 agtcatgaca caggctaatc gatacgcgct cgacgcactt cacctttctc cggaggtcat
     4261 cgtcggcaag cctctgcagg acgtgctaac aggcacaggg ttgtacgaca cactaggaag
     4321 cggaatcgag atcaagcatc acagaactcg cctgaagcag cactccggag acacgatcga
     4381 tgccatatgc aacgcgtatc cggtggcagt cgacggcaag accctcggag ctgtgttgac
     4441 ttttcagcgc ctagacgacg tgtcgaggtt cacatacgag atctgggagc acgagaacag
     4501 ggcgacctcc ttcgatgaca taatcggcga ttcgaggccc atgtcgtcag tgaaggagtt
     4561 cgccgcaaag atcgctccgg gcaattccac agtgcttctc aggggagaaa gcggcaccgg
     4621 caagggcctc atggcccggg ctattcacag agcaagcagg cgaaaggacg gtccgttcgt
     4681 cgtggtcaac tgcagcgcca tccccgatac gctgctcgag agcgagctct tcggctacga
     4741 ggaaggggcg ttcacgggag cacgcaagaa agggaagctg ggccggtttg aacttgccaa
     4801 caacggcacc ctcttcctgg acgaaatcgg cgacatgcca ctccacctgc aagggaaatt
     4861 gctgcgtgcg cttgaagaca gaatgattga gccggtagga agcgtggaat ccaggcccat
     4921 cgatgtgcgt ataatcgccg ccactaaccg cgatcttgat gcgatggtag agagccggga
     4981 attccgcaaa gacctgttct acaggctcaa cgtcgttccc atctggatgc ctccgctcag
     5041 gaagcgaaga gaagatatcc ccatgttggc acggttcttc ttggataaaa attgccgcac
     5101 cgccggcaaa agcacagtgg cgttcagcga tgaagcgctc cgatgcatgc tcgcgtacga
     5161 gtggccgggc aacgtgcgcg agttatccaa cagtgtggaa tatgcggtga atatcgagtc
     5221 tgaatcgact gtaagcatag ctagccttcc gcagagaatc cgaacagccc caggccccgg
     5281 cagtaccgat aatgcagatg gcccacaagc cctggaagca ctggaacgcc aggccatagt
     5341 ggaggcgctc cagcgctttg atggtgagcc gcgcgccaag gagaacgcgg cgcgcgagct
     5401 tggaatacac gttactacgc tatacagaaa gatcgccaag tacaacctag cctagccgtc
     5461 agcttgccac atagagatcc tgagcgatat cctccatacc cagctccagg agtctcctct
     5521 tcgatggacg cccgtcttca tgcgaccact gcatggcgtc gagatactca gacagtatca
     5581 agccggagtc taccgagaac ccggcagtcg ggccttcctg ctgaggaggg tttccgagca
     5641 ttcggccggg tacatgagcc ttgatcaagt tgaccccttc gcggatgttg aaggcttgcc
     5701 tcatgttcga tatgcgctcg cccgcttcaa tcgcttccgg cacgcccata tcaactccca
     5761 tgactgcaga gagaaactcg ggcacgcaat ccacgtgggt agacaggtat ccgaacacac
     5821 atacccccgc gcagttgacc acgttgcata ggcattccag cgacttgagc tgcgtgcctc
     5881 tccccgcctg ccgctctcgg tcagacccgt aagctggtat ctcatcatca aacgcaggcg
     5941 gtttgcagaa ctgagacgcc tgtatgtgct tgccggggaa catccggtat atcacgctca
     6001 ttgtaggttc gaatctcgga tcatgcattg ctatggcttc gccttgtacg tgaatggcaa
     6061 aagactcagc gcccccgcca atgacctttg ctgccctttc aagccccatc gacaggactt
     6121 cgccaagtcc gcggccgagt gctatgtcct ctgccagctt cactatggcg tcagggttcc
     6181 cccatgacag ctcgaggccg cccgcgtcct ccttgcttat caggcctttt tcgtatgcct
     6241 ccatcgcgaa tgctatcatc gatccagtgc cgattgtgtc cagcccgtag ctgttgcata
     6301 tgtcgttggc cttgataatc gattccgggc tgctgttcat gcagttggac ccaaaagcgg
     6361 ctatcgtctc gtattcaggc tgatggctgt catgaacagc atacggacct tcttcgatgt
     6421 cgaacgtgcc gctgcacgca atcgggcatc cgaagcaacc gtacttctta gtcttgaact
     6481 tgtagaacct atcgtgatgc aacgcctcga tctgcgactg agtgaaatcg tgcaggctgg
     6541 cgccggccca gttcttcacg ggcgaatcgc cgcccagcgc acacaggccg aagagagcag
     6601 gagtcccaac catgtacagt tgcgacgatc ctacatgatc gaccctgatc tgtttcacat
     6661 acttgctcct cagcgccttg gccctctcgc tgtccgcgac cggaacgggc ttgtcgccgc
     6721 gaaccacgat ggccttgacc ttcttagagc ccagcactgc cccgaaaccg gagcgtgcgg
     6781 cagcccggcc cctatcgtgg attatgcagg aaaggaggct cccgcgttcc cccgcaggcc
     6841 cgatgcaggc cacctgggcg tcgcgaccga acaggccctt caaggtgctg tccacatcgt
     6901 acgtggtcat tccccagagc cctgatgcat ccctaagctc agcacggccg ttatcgatga
     6961 ccaggtacac aggggtctcc gattggcctc tgaagaacac catgtcgtag ccggcgcgct
     7021 tcagagtagg gccgaagtac cctccggagt tcgcgtctcc ccacgtaccg gagacgggcg
     7081 atttgcccat cacagtgaag cgcgaaccta tcacggcggg gctccccgtc agtggtccgg
     7141 tcccaaacgc cactatgctc tcgggagcca gcggatcgag accggcttgt tggttgtcgt
     7201 atatcatcct cattcctatg ccgtaaccgc caatgtagtc ttggcatatg tcatcatcca
     7261 agcgctggtc tgtcagtttt ccagtagaca gatccaccca tagtactctg cccatgtatc
     7321 caccgagcat cgccaaccct ccgttcatgc cctcatctcg taggacttgt acatgtatac
     7381 ctcgacttct tggtgcaact tctatccctt gaccgctcct ccagttagtc cgctgacaag
     7441 atgcttctgg atgaacatga acagcacgat cggaggaatg gtgaacagca cggcggccgc
     7501 catcatgtcg ctccactgga tggtgaactc ttctcggaaa gagcttaggc cgaccgacat
     7561 cacccagttc ccctggctgc tcacgagggt acgcgcaaag aagaactcgt cccaggccag
     7621 gatgaaagcg aatatcgata cagccactat ccctgggagc accagcggca ttatcacccg
     7681 ccgaatcatt tgcccaacgg tgcagccatc tatcatggct gcctcgtcca gctccttggg
     7741 gatggcgtca aagaagcctt tcagcaccca tatgctcata ggcaacgtga aggcagtgtt
     7801 tgcgactatc aacccgccca gcttgtcgtg gagcccggtc cgtctgaaca gcacgtatat
     7861 tgggatcgca agaagcgatg cagacagcat ctgcgtcccc aaaatagcaa gcccagtgat
     7921 agagttcagc gttgtcttgt aacgtgagag gctgaaggcg gcgcccgcag ctagtggcat
     7981 tgataggaga acagtaccaa ctgcgaccat cagggtattc acaaaccatt gccctatcgg
     8041 cttggcttcg caaatcctcc tgaatgcgtc gagcgacggg gctttgggca gcaggctcgg
     8101 ccgctcggtg aacatgctct cactcgttat tgactgcaca aacatccagt acacggggaa
     8161 gttgagggca aaaacaacga ttatcgcaac aaccagaaca gctatctttc cctttggtcc
     8221 attcatgccg ccctcaagcc tcctctctca tgaaaagcct agagtagacc aacgtgatca
     8281 caagtgtaaa caacagcgca agtatcccca cagccgacgc cgaaccgaga cggaagtact
     8341 tgaacgcctc aagataggtg gttaccggca gtatctcagt ggctcgcgca ggccctccgc
     8401 ctgtaagaag atagattatg gagatccgct tgaatgtgta gagtatcaac atcatgacca
     8461 gcactgagtt cacgggcttg aggcccggca aggtaacgta tataaactgc tgcagcttgc
     8521 tcgctccatc cacgttggca gcctcgtaga gctctcgagg aatggcttgc agtcccgcaa
     8581 ggagaactac cgtggccagc gggaactgct tccatatcgt tacaatggtc actgtccagg
     8641 gcgccacggc tgaatcagtg acccatccta tctgcgatcc cagcaggctg ttgacgatgc
     8701 cgaactggta atcgagcatc cagttccatg tcattaccgc agccacctca gggatagccc
     8761 acggcagcag cactagtcct ctcagtatcg ctcttcccgg aaactccata ttcagtagca
     8821 gggcggtgat cagcccaatc gcgtatgagc cgacggttac tccaaacgta tatacaagcg
     8881 taatccagag tgaacgccaa aactcgggat tgctcagcca atcggcgtag ttggacaacc
     8941 ccgcaaatga cggcggtgaa agcgaaatca agggcgcatc cgtcaagctc agccacatgg
     9001 tcgtcagtat cggatagatc accactgcac ccatcaccat ggcggcgggc agaatcaggg
     9061 cagatgatgc tttgttgccc gctgcagcgg cgttaggcga cgctgttcgc attcggctca
     9121 ccaagaatag cctcctaaac tatgactaac tactaacatt gatgatcgtg agagggcgga
     9181 tttccccatc cgccctctcc atgtgtcgtc ttgctaccgc ttcagcgtgg cagcccactt
     9241 caccatcttc tgctgcacgt cgttcatggc cgcttcaaca gtggcccgcc cgccccacac
     9301 acgagcgatt ccatctacga ccattcgctg aacttcgccg gcatactcct caaaaccagg
     9361 ggccgagtag ccgaaccctg cagggtactt ggcggcaaca tccaccatac cctcgatcca
     9421 cgggtgctcc ttggcaagct tagggctgat ctcagccttc tgccccggga tctgcagcag
     9481 atcttcaagc catctgctct gccactcagg ctggttgtag acggagagta gcgcaaacgc
     9541 ttcctgcttg tacttggcac gaacaggaat ggtgtagaaa gcgccacccg tgatcgccgc
     9601 atgggttgga gtaggcggag ccgcaaaccc cacatacttg tagaggtccg gagctgcttc
     9661 cttcacatac gtgatcgccc aaggcccgtc tatgagcatg gcgatcttcc cctcgacgaa
     9721 catcttccgg agcattggac catcaaggcc cttcggggtc aggtcttcgt cgaagaaacg
     9781 cttcaggaaa ttcaggccct ctatggtctt gggatcgttc gctgtgggca cgccgtcttt
     9841 ggagaaatcg cccccgaaac caatagccca ctgcatgagc gggatatagg cctcaaggac
     9901 atctgctgtt ctggtggcga agccgtaccc gaaggcgccg gtagccttct tcaccttccg
     9961 cgcagcctcg agcatttcct cgggagtagt aggtaccccg acacccgctt tgtccagcaa
    10021 ctgcttgttg tagatcagcc cgttgggcga agccgtcagg actactccat aggtcttgcc
    10081 cttgtactgc gccaccttct ggaccggaag caggttgtct ttccattcag ccttggcaag
    10141 gtagtcgtcc aggggttcaa gcaagcccat gcgcatcagt ttgggcatca tgttggtgaa
    10201 cacaggcgcc aagtctggcg cgcctccacc cgccatgtcg atgagcagtt tttcctcata
    10261 ctgacccgct ggaaccagag cgggcgccac cttcactccg gggttctggg attcgtaaag
    10321 cgctacgccg tctttgaacc aatcaccggt tccggcttcg ccccacatcc agctcgggaa
    10381 ctgaatctgc ttgtttgcgc cgctggcgat tccaatcggc gccatgacca gagctatcag
//
LOCUS       DAOTAC010000055        10348 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_5481_length_10348_cov_4.490139, whole genome shotgun sequence.
ACCESSION   DAOTAC010000055 DAOTAC010000000
VERSION     DAOTAC010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 10348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10348
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5481_length_10348_cov_4.490139"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            745..4638
                     /locus_tag="PLM74_03730"
     CDS             745..4638
                     /locus_tag="PLM74_03730"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HON42226.1"
                     /translation="MRIPIRRRAALIVALAAFAVTAIMLASCWASAAPNLPYIAKTSP
                     GSEEVVRVDRERRTPEYVDGEIIVKLRPVFMMAGEGAGSAADGFRSLNARLGAVVAET
                     LPIGEIQEAYLVRIPAGMSVEEAVQCYAANEFVEHAVPNFLRYPDAAVFPNDAWFVQK
                     RMWGMLNQGQDFYPGVFWEKGIPGADIHATEAWGVRTDASSILVAVIDTGVQVSHVDL
                     AGNIWRNPGEIPDNGIDDDENGFIDDVYGWDFFNDDNSVYDDQVVDRHGTHVSGTIGA
                     VGNNGIGAVGVAWKARIMSCKFLGAGGGSDADAIKAINYARQMGAKIASCSWGGGGYD
                     PVLEMAMFESGMLFVCSAGNSATDNDIEPHYPSSYDLPNLISVAASDWNDNLASFSCF
                     GAESVDLAAPGYWILSCAPVNRGLWMGGTSMATPHVSGAAALVAAEYPDMPLYPGAEG
                     WSEGDLTVKDALLLSVDGNPAFAGKMTTGGRLNVANAIKLEFPVRITEASADATFGDA
                     PLHVNFSAAVEYPIAIDEAWWSFGDGSDDADGYTASHTYEEEGTYLACFHAVTCEGVE
                     SAWPMQIVVANPGTIILVDDDGGFNFEAYFLYACEQAGLDCVVVDSRYPLGLPDDFTD
                     RMLVWNTSFSWSDTLLPDQEDYLARFLDNGGRLLIISPEYFYDLGLTPFAWDYLHVWD
                     YDDNVPMEHWDGIEGDVITDGMSFYGDIGFGLEDALWPDFNARPILKNEDYGIEIWPA
                     LRYLDETYRVVFTTVPWEELPIFDPGEDGDPINPDPNNSAYFLTKIYNYLMGEINTPP
                     TVDKIEADIYFAEVGEEITFTAQAHDADGDELTYLWEFDSPGEPIDPVEGNSFKMAFE
                     APGVYAVILTVIDEHGEFRKELIAVTAVNPGCIVFVDDDDSDDDTEEYFFAAFDAIEQ
                     EYLAIMPSIAAGKYGGLDGLERFRVVWNCGELGGLDAAEQAAIASILGNGGRLFLAGQ
                     EVMFGLDKSAEGKAFAREYLHVTGVEHDVKTKYVTGVAGDPITGSAGQVGLTFPEGFD
                     DWTDTLTLAPEAQAIFLNDKSKPCALRYVGDKHRLVFMAVAFEAFPLEQADEPTGRTG
                     VRNGTPLFDAASLLDNVLEWIDIPVVAVTKPAQGQVCTGFANISWIAHDYAGSELVIK
                     IEYRMADGSTWIPIASGEANDGSYLWDLTKLSKSGQYVVRVTADRSDGVSVSGDSRPF
                     VVSVVAANRFAAGPNPASDAVNFYINASGEATLYVYDIAGRQVFSQKIENGQTYFEWH
                     LVNNAGKPLANGLYLCYMVTADGVRTDITRLVISR"
     gene            4653..5549
                     /locus_tag="PLM74_03735"
     CDS             4653..5549
                     /locus_tag="PLM74_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42227.1"
                     /translation="MKTKHANWMVIAVMAATLLLVLAPAAALAAAEDPVGTAALFDVG
                     MGARALGMGGAFVAVVDDANALYYNPAGLALIEGRDVTSMYSTLFGAGNYLGVGYAQK
                     NIGAAVFGLLSAATGTDEYGNPTDEFGYREGTLAGGYAHAFGPFALGGAVKLYAQDAS
                     TNRGFGATGDIGALITLPYLDGIRFGAVARNLIGSVKFESGHTDSFDKSYVLGLSVKP
                     LKGLTLAADYDITRSTGRVGAEYQIIDAFAARAGATVNADKQWGFTAGAGFAFAGFRV
                     DYAYQFHAELPDAHWVSLGYSF"
     gene            complement(5750..6529)
                     /locus_tag="PLM74_03740"
     CDS             complement(5750..6529)
                     /locus_tag="PLM74_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HON42228.1"
                     /translation="MNERRLVTAQELADMLQLSVETIWRYTRNERIPYVKLSSRQYRY
                     DPDKVMHRLGMNAGGHGSGSVCEAGPEYMTDKVYTYEDYLQLPDEEIYHYEILDGMLI
                     REPAPSVHHQRVSRRLQRLLEEYFASRDSTGEVFDAPIDVTLSDTNVVQPDLVYVPGA
                     CSEIVEEARIDGAPYLVVEILSKWTRSKDRIRKRNIYERMQVPHYWVVDPEDETIEAY
                     ALRDGAYVLRSSGLSEGEFVHPDFPGFAFDLAALWRRPGTA"
     assembly_gap    6643..6742
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7844..8815
                     /locus_tag="PLM74_03745"
     CDS             7844..8815
                     /locus_tag="PLM74_03745"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HON42229.1"
                     /translation="MEYRVLVTPAFFGRKNLTPIHLLSEAGCEVVPNPHGRTATEDEM
                     CALVADVDAAIVSLDRVTEKVLASGPRLRVVARCGVGYDNIDVAAATRMGIFVTNVPG
                     ANSRAVAEMTVGLMFDVARRISMMDRRMREGIWKGHVGFELGGKTLGIIGTGSIGKLV
                     AELGVGLRMRVLCYSRTHDRIWADAIGAAYVSLDELLRTSDVVSLHIALTDDTRNLIS
                     ERELSLMKPSAILINTSRGAALDEDALYVALREGRLSGAGLDVFVEEPLDQSRLLVLE
                     NVVLTPHISGLTVESAHAMAMGAARNVVAVLHGRTPENAVNAPRASG"
     gene            8894..>10348
                     /locus_tag="PLM74_03750"
     CDS             8894..>10348
                     /locus_tag="PLM74_03750"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HON42230.1"
                     /translation="MHTAHTWYTQHASQSSRIQPELHTSPLHVPKSGDSTSFECGTCP
                     PVRRFDVSISIGGDAGQGVESAGAIACRALVRAGYFAFGMADYRSRIRGGTNFYQVRA
                     SDAPVLCHGDPVHVIATLTRDAAFTQLGNLARGGALVCDESVDLEGSIRPDVRVLSVP
                     ITNIAENAGSKASMNMAALGAAMGLVGFSIDTLCAAIEQRFARKAESVVRANVQAARE
                     AHEYVTGRFAESFPFRLHPADPPSASPPASPSMSTSSSPSHGRILLSGNDAFCLGAVA
                     GGCRFIAAYPMTPATTILEWMAAHEGDLGIVAVHAEDEIAAACMAVGASLTGARAMTS
                     TSGGGLCLMTETCGMAGMTEVPLVIVDVQRGGPSTDLPTRTEQSDLLLAFHPSHGDFP
                     HIVVAPGTVQQCFEAGYRAFNLAERYQCPVIVLLDSYIGGSLVTLGRSCLSWNEVVRD
                     RGEYVGGYNADVEATEAEAETETGAGAADAEVHVD"
BASE COUNT         2053 a         2860 c         3190 g         2145 t
ORIGIN      
        1 ctcttcgcct ggccgtcaaa acctcgcaca ttctgaggag gtacgtcgga aaaccgccca
       61 tatttgctgc aggctcgcta atcccctgtt aaacgcccgt ttagccaaag gcctgcgtaa
      121 agcctattct gcctgtcact ggcgtttccg agcctaagca ggtcgccggc ctggccgtcg
      181 gtcggctccc tcggtctggc taccgatggt acgtgcatca gcgttttccc gttcagctgc
      241 aatatgtgga tatccggggc gcttcggcct ttgtatctgg gccgatccat ggatacgggt
      301 aacgtgtaat ccctgtaacg ggggatacct gcagagccta ctcgagcgcc caatcctgtg
      361 ccgcaacgaa cgctttgcgc ggcgtcaagg agtctgccga gtggcggcgc acagtcccgc
      421 ctcatctggg ttcgacataa tcccacgccc aatccgatca tgctcgcgcc gttgtgcgta
      481 cacacgtgtc ctatcgactc cacattgcgt gttgtacttc ttgggcattt gtacagagct
      541 acatagtatt ctgtgcccta gtatcgacat ttatgggagg aaatggtaca agcttgtcga
      601 aatcccaatg tgtatgaccg ggctacttac gtcctactgc aaatcgatat gcggaggtga
      661 tagacgctct gcagtctttc gatcgacccc atgatgagtc tttctgaagc gcaagtatgc
      721 gactacacaa ggggggtaag tgtgttgaga atcccaattc gaaggcgtgc cgcgctgatt
      781 gtggcccttg cggcatttgc ggtgacggcg attatgctag caagctgttg ggccagtgca
      841 gcgccgaacc tgccgtacat tgccaaaacc tcgccgggtt ctgaggaggt cgttcgggtc
      901 gatagagagc gccggacgcc agagtatgtc gacggcgaga ttattgtgaa actcagacca
      961 gtgttcatga tggcgggtga gggcgctgga tctgcggctg acgggttccg ctcgctcaat
     1021 gcgcggctgg gcgcggttgt cgcagaaacc ctgcccatcg gcgaaatcca ggaagcgtac
     1081 cttgtcagga tccccgctgg catgagcgtg gaagaggctg tccaatgcta tgcggccaac
     1141 gagttcgttg agcatgcggt gcccaacttc ctgcggtacc cagacgccgc agtgttcccc
     1201 aatgatgcct ggtttgttca gaagaggatg tgggggatgc ttaaccaggg gcaggacttc
     1261 tacccggggg tcttctggga gaaaggtatc ccgggcgcag atatccatgc tacggaggcc
     1321 tggggtgtgc gtaccgacgc atcttcgatt ctcgtggctg taattgacac cggggttcag
     1381 gtgagccacg ttgatctggc aggtaacatc tggcgcaatc ccggcgagat accggataat
     1441 ggcattgacg acgacgagaa cgggttcata gacgatgtgt acggatggga cttttttaac
     1501 gatgacaatt cagtctatga cgaccaggta gtcgaccggc acggaacaca cgtcagcggc
     1561 accataggcg ccgtgggcaa caacggcata ggcgctgtcg gcgttgcatg gaaagcacga
     1621 attatgtcgt gcaagttcct aggcgcaggc ggaggcagtg atgctgacgc catcaaggcg
     1681 atcaactatg cgaggcagat gggcgccaag atcgcaagct gctcctgggg tggaggcggg
     1741 tatgaccctg tgcttgagat ggcgatgttc gagtcgggta tgctcttcgt atgctcggca
     1801 gggaacagcg ctacggataa cgacatcgaa ccccactatc cctcctcgta cgatctgccc
     1861 aatctgatat cggtggcggc gtctgactgg aacgacaacc ttgccagctt ctcttgcttc
     1921 ggagcggagt cggttgacct tgccgcgcca gggtactgga tcctgtcttg cgcacccgta
     1981 aaccgcggac tgtggatggg cggtacgtcc atggccactc cgcacgtgtc gggcgcggcg
     2041 gccttagtag cggccgaata cccggacatg cctctctatc caggggccga gggatggagc
     2101 gagggtgacc ttacggtaaa ggacgcgctg ctattgtcgg ttgatgggaa tcctgctttt
     2161 gcaggcaaga tgaccaccgg cgggcggctg aacgtggcga acgccatcaa gctggaattc
     2221 cccgtgagga tcactgaggc ctctgccgac gcgacctttg gggatgcgcc tcttcacgtc
     2281 aacttctctg ctgcggtcga gtatccgatt gcgattgatg aagcctggtg gtcttttggg
     2341 gacggctccg atgatgcaga cggatatacg gcgtcccaca cctacgaaga agagggaacc
     2401 tatctggcct gcttccatgc tgtcacctgc gagggcgtgg aatcggcttg gccgatgcag
     2461 attgttgtag ctaaccccgg gaccatcatc ctcgttgacg acgatggagg gttcaacttc
     2521 gaggcgtatt tcctctatgc atgcgaacag gccggcctgg actgcgtcgt ggttgactcg
     2581 cggtacccac tgggcctgcc tgacgatttc actgaccgga tgctcgtgtg gaacacttcg
     2641 ttctcatgga gcgatactct gctccctgac caggaggact atctggccag attcctggac
     2701 aatggcggca gactactcat tatttcaccg gagtacttct acgacctggg ccttactccg
     2761 tttgcatggg attatctcca tgtatgggac tacgacgaca acgtaccgat ggagcattgg
     2821 gatgggattg agggagacgt aatcaccgat ggtatgagtt tctacggtga catcggattt
     2881 ggcttggagg atgcactctg gcctgacttc aatgcaagac caatcctgaa gaacgaggat
     2941 tacgggatcg agatatggcc ggccttgcgg tacctggatg agacctaccg tgtggtcttc
     3001 accaccgtgc catgggagga actccccata ttcgatccag gagaggacgg ggatccgatc
     3061 aacccagacc ccaacaactc cgcgtacttc ctaacaaaga tctacaatta cctgatgggc
     3121 gagatcaaca cgccgcctac agtcgacaag atagaggcag acatctactt cgcggaggtg
     3181 ggcgaggaga tcacatttac cgctcaggct cacgatgcgg acggcgatga attgacctac
     3241 ctgtgggagt tcgatagccc cggggagcct atcgatccgg tggagggcaa ctccttcaag
     3301 atggctttcg aagcccccgg cgtttacgcg gtcattctga cagtgattga cgagcatggt
     3361 gagttcagga aggaactcat cgcggtgacg gcggttaacc cgggctgcat tgtgtttgtc
     3421 gacgacgatg attcggatga cgatactgaa gaatacttct ttgcggcatt cgatgctatc
     3481 gagcaggaat accttgcgat aatgccctcg attgcggcgg gcaagtacgg tgggctcgac
     3541 ggccttgagc ggttccgcgt ggtatggaac tgcggcgagc ttggcgggct cgatgcggca
     3601 gaacaggcgg ccatagctag cattctgggc aacggcggga ggttgttcct agccggccag
     3661 gaagtaatgt ttgggctgga caagtctgcc gaaggcaagg ccttcgcccg cgagtatctg
     3721 catgtgaccg gagttgagca tgatgtcaag accaagtatg tcaccggcgt cgcgggcgac
     3781 ccgataaccg gaagcgctgg tcaagtaggt ctgacgttcc ctgagggctt cgatgactgg
     3841 accgacaccc ttacgctggc gcccgaagcc caggcgatct tcctcaacga caagagcaaa
     3901 ccttgtgcgc tcaggtatgt cggggacaag catcgccttg tgttcatggc cgtggcgttc
     3961 gaggctttcc cgcttgagca ggccgatgaa cccacgggca gaaccggcgt gcgcaatggc
     4021 acacccctct ttgatgcggc gagtctcctc gacaatgtac tcgagtggat cgacatcccc
     4081 gtggtggcgg tgaccaagcc tgcacaggga caggtctgca ctgggtttgc caacatcagc
     4141 tggatagcgc atgactacgc cggcagcgaa ctagtcatca agattgaata ccgcatggct
     4201 gacggaagta catggatccc cattgcctcc ggcgaggcga acgacggctc ctacctgtgg
     4261 gatctcacga agctcagcaa gagcgggcaa tacgtggtcc gagtgaccgc ggataggtcc
     4321 gacggcgttt cggtaagcgg cgatagcaga ccgttcgtag tgtcagtagt cgcggccaac
     4381 aggttcgctg caggcccgaa tcccgcgtcc gacgccgtca acttctatat caacgcttcc
     4441 ggcgaggcaa ctctgtatgt gtacgacatc gccggtcgac aggtcttctc acagaagatc
     4501 gagaacggcc agacctactt cgagtggcat cttgtgaaca acgccggcaa acctctggcc
     4561 aacgggctct atctgtgcta catggtcact gccgacggtg tgagaactga catcacgcgg
     4621 ctcgtgatca gccgctaagt gggggtgacg agatgaagac gaaacacgcc aactggatgg
     4681 tcatagccgt aatggctgcg acgttgctgc ttgtgctagc gccggcggcg gccttggctg
     4741 cggcagagga tcccgtgggc actgcggccc tgttcgacgt gggaatgggc gcccgggcgc
     4801 tcggaatggg tggcgcattc gtagctgttg tcgacgatgc caacgcgttg tactacaacc
     4861 ctgcggggct agccctgatc gaggggcgtg acgtgacttc gatgtactcg actctgtttg
     4921 gggccggcaa ctacttggga gtcggctatg cacagaagaa cataggtgcg gctgtattcg
     4981 ggctgctgtc ggcagctaca ggtactgacg agtacggcaa ccctactgac gaattcgggt
     5041 accgcgaggg aacgctggcg ggcggatacg cccacgcttt cggcccattc gcgttgggcg
     5101 gggcggtcaa gctctatgcg caggacgcaa gcacaaaccg aggtttcggc gcaaccggcg
     5161 acataggggc gctgatcacc ctaccttacc tcgacggaat caggttcggt gcggtggcgc
     5221 gcaacctcat cggctccgtg aaattcgaga gtggacatac tgacagcttc gataagtcgt
     5281 atgtattggg cctgtcagtg aagccgctta agggcctcac gttggcggct gactacgaca
     5341 taacccgctc cacgggccga gtgggcgcag agtatcagat catcgacgca tttgctgctc
     5401 gggcaggcgc gacggtgaat gccgacaagc agtggggctt caccgccggc gcggggtttg
     5461 cgttcgcggg cttccgtgtg gactacgcct accagttcca cgcggaatta cctgacgccc
     5521 attgggtatc gctgggctac tcgttctagg aggcccgtgg gcagttccgc aggtgatgcc
     5581 gcgcgatcga gaagcgcggt gagggttgcc ggcccatctt ctgggcgggc agccctcctt
     5641 ctgcttatgc catgagctca ctggagttcc ccgcgagttg accgggctgc cgcagaccgg
     5701 gctgcgaggg gcaggccatg ctgcttcggg atggattcag agaagcagac tatgctgttc
     5761 caggccgtct ccacagggcc gcgaggtcga atgcgaatcc tggaaagtcg ggatgcacga
     5821 actcgccttc gctaagcccc gagcttcgca gaacatatgc gccgtcgcgc agggcatagg
     5881 cctcgatagt ctcgtcctcg ggatcgacca cccagtagtg cggcacttgc atgcgctcgt
     5941 atatgttgcg ttttctgatt cggtctttgg accgggtcca cttcgacagg atttccacaa
     6001 ccagatatgg ggcgccgtcg atgcgtgcct cctccacgat ctcgctgcat gcgccgggaa
     6061 catagactag gtcgggttga acaacgttgg tgtccgacaa cgtcacatca atgggcgcgt
     6121 cgaatacttc gccggtcgag tcccgggaag caaaatactc ctccaaaagg cgctgcagac
     6181 gtctggacac tcgctgatga tgcacggacg gcgccggttc gcgtatgagc atgccatcga
     6241 ggatctcgta gtgatagatt tcttcatcag gcagctggag gtagtcctca tatgtgtaca
     6301 ccttgtccgt catgtactcg ggcccggctt cgcacactga accggaccca tggcctccgg
     6361 cgttcatgcc caggcggtgc atgaccttat ctgggtcata ccggtactgc ctgctagaca
     6421 gcttaacgta tgggattcgt tcgttgcggg tatagcgcca tatggtctcc accgacagct
     6481 gaagcatgtc ggccagctct tgcgctgtta ctaacctacg ctcattcaca ggagtacccc
     6541 tttcatatcg ccaggaacct acgtcgcctg agccggacgc ttgcagaggg ccgcgaggtc
     6601 gaatgcgaac cccggaagac cgacgtactc aggcctggtt ctnnnnnnnn nnnnnnnnnn
     6661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6721 nnnnnnnnnn nnnnnnnnnn nngggccgcg aggtcgaatg cgaaccccgg aagaccgacg
     6781 tactcaggcc tggttctacg cgtaccctta ctcacggcga ctccctcccc tcaggcaaag
     6841 catacagggt cacgcaagta aaagcaagtt ataacaagta atgataagcc atagagatca
     6901 gcggtgcgct gcactcagcg tggcgctcca gattttgcgc ggcgcctcgg attgtgtgta
     6961 gcacttcgga ttgtacctgg cacttcggat tgtgcctggc accttcccgg ggtgtactcc
     7021 tgtgccgctc agctggctaa ccgtaactcg caatggccaa ccaaacccaa ggtgaaacga
     7081 acccatcccc atgggtacgc gcgttttcgg tcatggacga agcgaagcct ggcagcaggt
     7141 aaggcgtaac ggcagactac atacagggct aagggcacga tctaggcgtc gatcatatga
     7201 atgcagtgta tccatggata ttcctgggag taacgctctc ctgcggtggg ccccagcggg
     7261 gctctgaggc atcctgtcca tttttgaacg ggcttttcga gtgtcctgtt cagatcctac
     7321 ctggtttggc aggcgacaat tatctgaatt cgcaccggca ggggttcggc ctttttctgt
     7381 aatcacgatg tcgctaaatc cgcaggcaga ggagtctgta ataatctggt cacgcgcctc
     7441 tatccctcga atgtgcccga gacagagtta ggcgactgcg aattctccac gaaatgccag
     7501 caattcccgg gttttgccgg ctcccggtca aaaccgggca cattctgcgc aggtacctcg
     7561 gaaaaccggg cacgtttctt gcaggtctgc caactccctg ctaaacacgg ttttgcccaa
     7621 aggcctgcat aacgccgctt tcgccgatga ctggcgtctc cgagcgtagg caaatccccg
     7681 gcgcgaggtg ttgattcgca catcggctgc ccatgccgag agaaggggat ggcgcccgag
     7741 tctcgaagaa gatgcacgca aggcggtaga ggcggtcgcg atgtgcagcc gccaaccccc
     7801 attacccata cctgatgact atcaggccgg ggaggtccgt gccatggaat acagggttct
     7861 cgtaacaccc gcgtttttcg gccgcaagaa cctgacgccg atccatctgc tcagcgaggc
     7921 cgggtgcgag gttgtgccga atccacacgg gcgcacagcc acggaggacg agatgtgcgc
     7981 tctggtggcg gacgtggacg ccgcgattgt cagtctggat cgggttaccg agaaggtgct
     8041 tgccagcggt ccgcggttga gggtggtggc aaggtgcggg gtgggatacg acaacataga
     8101 cgttgcggcc gccacgcgta tggggatatt cgtgacgaat gtgcccggtg caaatagccg
     8161 tgcagtggcc gagatgaccg tcgggctcat gttcgatgtg gccagacgta tctcgatgat
     8221 ggatcggagg atgcgggaag gcatatggaa gggacatgta gggttcgagc ttggagggaa
     8281 gactctgggg attatcggga cgggcagcat cggcaagctc gtggccgagc tgggtgtggg
     8341 gctcagaatg agggtgctct gctattcacg aacacacgac cgcatctggg ctgacgcgat
     8401 tggagctgcc tacgtgtcgc tggacgaatt gctcagaaca tcggatgttg tgagcctgca
     8461 cattgcgttg acagacgaca cacgcaacct gatttccgag cgcgagttat cgctgatgaa
     8521 gccgtcggcg attctgatca acacatcccg cggcgcagct ctggacgagg atgcgctgta
     8581 tgtagcatta cgggagggcc gcctgtcggg agctgggctg gatgtgttcg tagaagagcc
     8641 cctagaccag tctcgcttgc tcgtgctgga aaacgtggtg ctcacgcccc acatcagcgg
     8701 cctgacggtt gaatccgctc acgccatggc gatgggcgcg gcgaggaatg tcgttgcggt
     8761 tctgcatggc cggacgccgg agaacgcggt aaatgcaccg cgagcgtcag gataacatga
     8821 ccgaaatcgc gtaacatgtt ccctcacgaa ctgggcagaa tgagactgac ccagaagtcg
     8881 ggaggggcag tcagtgcaca cagcacacac gtggtatacg cagcacgctt cacagtcgtc
     8941 gcgcatacag ccggagctac atacatcgcc gctacacgtg cccaagtccg gtgactccac
     9001 gtccttcgag tgtggtacct gccctcctgt gcgccggttt gacgtctcca tatccatagg
     9061 gggcgatgca ggtcaaggtg ttgagtcggc cggcgccata gcctgccgtg ctctggttcg
     9121 ggcaggctat ttcgctttcg ggatggcgga ctaccgatct aggatccgcg gcggaaccaa
     9181 cttctatcag gtccgagcga gcgacgcgcc tgtcctttgc catggcgatc cagtccatgt
     9241 cattgccaca ctcacgcgtg acgctgcctt cacccagctg ggcaacttag ctaggggcgg
     9301 tgccttggtc tgcgacgaat ctgtcgatct tgagggtagt ataaggcccg acgttcgcgt
     9361 tctttctgtc ccaataacca acatcgcaga gaacgcaggc agcaaggcat cgatgaacat
     9421 ggccgcgctc ggggcggcca tggggcttgt cgggttcagt atagacactc tgtgcgctgc
     9481 aatagagcaa cggtttgccc ggaaagccga gtcagttgtg cgtgcgaatg tccaggccgc
     9541 gcgggaagcg catgagtatg tcacgggccg atttgccgaa agtttcccgt tcagactgca
     9601 tccggcagac cccccgtctg catccccgcc cgcgtctccg tctatgtcca cgtcgtcatc
     9661 accttcgcac gggcgcattc tgctgtcagg taacgacgcc ttctgcctgg gagcagtggc
     9721 tggaggctgt cgtttcattg ccgcctaccc gatgactccg gctactacga tacttgagtg
     9781 gatggcggcg cacgagggcg atctgggcat tgtggcggta cacgccgagg acgagatagc
     9841 tgcggcctgc atggccgtgg gggccagcct gaccggtgcg cgggcgatga cgtccacgtc
     9901 ggggggcggt ctttgcctga tgacagagac atgcggcatg gcggggatga ccgaggttcc
     9961 cctggtcatt gtggacgtcc agcgtggggg gccgtctact gacctgccga cccggaccga
    10021 gcagtccgat ctgctgctcg cgttccaccc gtctcatggc gacttcccac acatcgtggt
    10081 cgcacccggc actgtgcagc aatgcttcga ggccggctac cgtgccttca accttgccga
    10141 acgatatcaa tgcccagtga ttgtcctgct cgattcgtat atcgggggtt ccctcgtcac
    10201 actgggccgg tcatgcctga gctggaacga ggtcgtgcgc gaccgcgggg aatacgtggg
    10261 cgggtacaac gcggatgttg aggcgacaga ggccgaagca gagaccgaga ccggggccgg
    10321 cgcagcagat gccgaagtgc acgtggat
//
LOCUS       DAOTAC010000056        10273 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_5537_length_10273_cov_5.117636, whole genome shotgun sequence.
ACCESSION   DAOTAC010000056 DAOTAC010000000
VERSION     DAOTAC010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 10273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10273
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5537_length_10273_cov_5.117636"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..988)
                     /locus_tag="PLM74_03755"
     CDS             complement(<1..988)
                     /locus_tag="PLM74_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42231.1"
                     /translation="MAHSSVLASISHAVMDGAGEQMALVVHSGDMAARARREVLSMTE
                     SGAALVIPVYTLDQFAAAILRRAGGRPRSRPIGELEREIVIGRLLKGWAGSGQLAALG
                     HSADRPGVARAFAGAILDMKQVAATPDQVEQALTGGSAPTGVHADFLAVWRDYEAFLE
                     GHGLADGEDLYIDAASALIAQGGQALGDLGRVWFDQFFDVTPAEGALISAVARFVPAT
                     CVGLACTQCDDAGSVIERARRLVEPATVEVSVRGGSLKPELSIITAPGPAAEAREVAR
                     EVKRLIVEEGRKPWEICVSSADSAEFIPALLEAFEEFGVACAAPRKRLLSSSM"
     gene            1980..2840
                     /locus_tag="PLM74_03760"
     CDS             1980..2840
                     /locus_tag="PLM74_03760"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HON42232.1"
                     /translation="MQIVTDHGADISPEQREGLDIHYVPLTITLGGRVYRGGVDISVE
                     EFYELLERTGEMPSTSQPSPGDFAELYRNLARTDPDILSVHISSGLSGTVNTARMGAS
                     MVPEANVTIYDTKTLSGAQGWHVEAAARAAKAGWSVDRIVGMLQRMTAATDTLYTLST
                     LKYLIHGGRISHLKGLVASILNIKPIIGVEKVGGTYVTRGQTRTLDRAIDTIVNMVAS
                     VYPANTVMRMQVLHGLCPEGAVRLRAGLDRIFQCRWLPVAPIAPVLGAHTGPGLVGVV
                     FAPEAQLPKF"
     gene            complement(2845..4602)
                     /locus_tag="PLM74_03765"
     CDS             complement(2845..4602)
                     /locus_tag="PLM74_03765"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HON42233.1"
                     /translation="MHVCSGSIRRRARVLVIAGIALILSGLCTCADAQATPPAIGSAR
                     SRQRITVMTYNIHAGLGVDFVYSLDRIADLIRAEQADLIGLCEVEQKTQKVNFDDQAK
                     LIADRLGFHHAYGPIFARPTGFFCNALISRFPILSHRVHQLPNPNRTQARAALEAQVD
                     VDGTIVTVFVTHLEVADSGSRVAQAEALADIASSTPTPRIIMGDFNATPTHALETALM
                     LREHNDTYALHRVLVDSRWLVALGPFEREYLKGGYTIGVFDPSARIDYIFASNDIGVA
                     GEIGIARVPRSLASDHLPYVVTLELPRKQEGQARLAATTVDATAETLGSRVLGPIRPL
                     PDSPDGCGPLVAVMRSEDVKAWYEDMGWYYEDDLDMIEEMVDGIGLGGLECALVPADE
                     LQSLVATVSRRPTLLVLSNARRMSERQMRAVRDFVAVGGRVLATYQTSLKTEAEAVAG
                     EYGFGLADLFGAELAGWTGVSPMHDAIAPIGKGEVDAANGSDAVIRAIWEGVGAPVRL
                     RVPEGMVTKPWPGSVVLGQWSDRTGAPSRALPLNAAIVLNGPVMYVGADLLSWDALQE
                     EPARALAANMVRFMLGVDH"
     gene            4706..5527
                     /locus_tag="PLM74_03770"
     CDS             4706..5527
                     /locus_tag="PLM74_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RMD1 family protein"
                     /protein_id="HON42234.1"
                     /translation="MNPTLFRAVAVSRELNLTTIALQFGIERPFSWEDTLVLSERHLD
                     GILPQPAGKGVYLFSFGTLVFVNCADSDIMDVIAYLKKLDRNLGEFARVEYVDDYKLT
                     VSGDELSVTYDELITPEMKDYYRELVAVILAKSTAMERIEDEISKLFDDVEQFITQLK
                     RGTLSISDERLARLSGRILSYRYSSISYIMILEKPDVVWRSQEAQELYSDLERLFELP
                     DRYEKISHKTQTLLDMTQVFADLYHAKRGTRLEVAVILLIAFEIILSLVERFWPF"
     gene            complement(5496..7001)
                     /locus_tag="PLM74_03775"
     CDS             complement(5496..7001)
                     /locus_tag="PLM74_03775"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HON42235.1"
                     /translation="MAIFAVRRRGRTSCRHALVGLVFALLAVSALDGPVRAERADEVD
                     LGNIAGIICELSLPKYAGRLTGTDGNWAAVNYVADCFRRLGLASPDGLDGYLQPYIQP
                     TRLMHSPPRLEIVGASGSVARQFRFITQFSPSTAPGCRVSGQVTAPGVVLQHAADIDR
                     VRGEAVLLVPMHLAGNPAEIEAIMKRVAASSGAGQAEFVGVVLEIDTAGRGYFPIVPY
                     IAPSAASADQGDGGQQPTAESTANEPMVFTCDRPTFAELAAWSGAAPAATPTAIATAT
                     SAADSPSGTIRMSMDYSIEPVRTANVVGVLPGGAHHIIIGAHLDHVGSNMDGTYNPGA
                     MDNASGVAAIIEIARALSVGPVRPSLTVVFVAFNGEEQFMYGSRHYVSEPCLPLGHAA
                     MLNIDTVGSPRGVLLQIENASGHDTQLARDLGRVASKLGIPFRKSMSSGSDHSSFANA
                     GVEAVNLIQPDFAGGYHGPGDTADQVDPGRVAEVVRLVLEYIRTAKTAPPD"
     gene            complement(7259..7633)
                     /locus_tag="PLM74_03780"
     CDS             complement(7259..7633)
                     /locus_tag="PLM74_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42236.1"
                     /translation="MLKRILVIALPVGLIALFIAVMLSAAVLKHPLGSADDVPAWLSR
                     VEAHVMDEQWQDALEGARKLADAFNRVTVRVQFSAQLHDVLDLRAAIARAEGFIRAQN
                     RAGAMAELAEARTLWDRIGSRH"
     gene            complement(7651..>8289)
                     /locus_tag="PLM74_03785"
     CDS             complement(7651..>8289)
                     /locus_tag="PLM74_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HON42237.1"
                     /translation="SQLTYFDYILGITIGSIAAALTTDLSSTAWPHWVGLTAWTAAGL
                     TMQWVTLKWRFASKYIDGEPTVVIMNGKIMEGAMRKLRYRASDLMEQLRVKDVFDIGE
                     VEFAILETNGTLSVLKKTQFQPVTRSDMSISTQYQGIGTELIYSGVIFDQNLRQVHLD
                     RSWLDSELRKKGIQDSRDVFLAVLDTSGNLYVDTYHDRVENPVDTGDYPGPY"
     assembly_gap    8290..8389
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8411..>10273
                     /locus_tag="PLM74_03790"
     CDS             8411..>10273
                     /locus_tag="PLM74_03790"
                     /inference="COORDINATES: protein motif:HMM:NF023835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase-associated domain-containing
                     protein"
                     /protein_id="HON42238.1"
                     /translation="MILHAAWLPFADAAAGAVPTASALAGNQAAPAPGGRLFIWGESP
                     ERFGLKLRGRKGPFPGHPFQASATELMQAFALLVAAAGCSLNRNQGTVGSAAGPDAVR
                     ASAKLVQKPRKSHALILLPTDDGYPIPSPALLKQARRVLPTARLERWTANGGSVGLHN
                     AGCFQATGVSISALDIIRLLTVLPQSRRDDGGDYPEPDDRLVRLRLSAAPGSDFAFWT
                     QASKLALELMAKQRFMPDICVSTDDSRARAVWRPLWESAQDVSRFRALADAMPLLCRA
                     VWPLEESGSARIVPSDVILRSFIDTSMDETVRGMLAMERNIRWADSIARQEFDDAERR
                     LLEMAMLSNKRPACAWICSLNKEKPEIRLEQARLDSVRRGISDWIAAASLRQAPASSV
                     PSVSAASGFRTCFRLEPPNTEADSADHECETDSAAEFWTLHYYLQAADDPSVLVPAAR
                     VWEESGGMLRFLGKRFENPHERLLADLGQAARHFPAIETSLDTATPESCELTTTEAYS
                     FLREVSPQLEQLGLGVLAPPWWERVSSGPARLGVRLKVRSESAQDAGAGEEANVAPRF
                     SLDSLVDYDWQVALGDEVMSKDEFRRLADLKVPLVRVRGEWVEINPREVESAIAF"
BASE COUNT         2061 a         3181 c         2923 g         2008 t
ORIGIN      
        1 gcatcgacga tgacagcaga cgtttcctcg gcgccgcgca cgccactccg aactcctcga
       61 acgcctccag cagcgccgga atgaactctg ccgagtccgc gctggagacg cagatctccc
      121 acggcttacg tccttcctcc actatcaacc gcttgacctc acgggctacc tcgcgggcct
      181 cggccgctgg gccaggcgca gttattatgg aaagttcagg cttcaaggac ccgccgcgaa
      241 cgctcacctc gacagtggca ggctccacaa ggcgcctggc tctttcgatt acagaacccg
      301 cgtcatcaca ctgggtgcag gcgagaccga cgcaggtcgc cggcacaaaa cgggctactg
      361 cggatatcag ggccccctca gcaggcgtaa cgtcgaagaa ctgatcgaac cagaccctcc
      421 ctagatcccc gagcgcctgc ccgccctggg ctatgagtgc ggaagcggca tcgatgtaca
      481 agtcctcccc gtcggccagg ccatgccctt caaggaaggc ttcataatcg cgccagacag
      541 ccaggaagtc ggcgtggact cccgtcggcg cactgccgcc tgtcaacgcc tgctcgacct
      601 ggtcgggagt ggccgcaact tgtttcatgt cgagaatcgc cccggcgaac gcgcgcgcga
      661 ctccgggccg gtctgcagag tggcctagag cggcaagctg ccccgagccg gcccatccct
      721 taagcagacg gcctatgacg atctcacgct cgagctcgcc aatgggcctg gatcttggcc
      781 gtcctcctgc cctgcgcaag atggccgccg cgaattgatc aagcgtatag actggaatca
      841 cgagggcggc gccggactcc gtcatcgaca gaacctcgcg ccgcgccctt gcggccatat
      901 cacctgaatg aaccaccagc gccatctgct cccccgcgcc gtccatcacg gcatgggaaa
      961 tactcgcaag aaccgatgag tgcgccatga gcccaacaca ccaccccaag ctccgttcgg
     1021 gcgcctgagc gcccggctat atctttcacc ttctgaggtg tgtatccctt ccgaatgtag
     1081 gtaaacaacc ccaaagcgaa gtcatgccgc cgtgaagtgc gatctggcgt ctacgcgcct
     1141 tatcactggt acccaaaccg cagcagccgg ccgaccaccg ggccagcgac ctacttaagc
     1201 tcggaaacgc tagttgcagg cgaaagaggt tttacacagg cctttggcga aacgggcttt
     1261 ttgcagggga ttagcaaacc tacaacgaat gtgggtggtt ttccgacgta ccttcttaga
     1321 atgtgcgagg ttttgacggt taggcgaagg atgagtacaa tcaagcgcgg acgggaagga
     1381 ggattttaga tgagctgggg tgtaatgatt gcggtactct ttgttctcgc ggttgccata
     1441 ggggttgcca tgaagaaggg caagtcatag ctgtcagtgt cgtagcttgg cggagtgaaa
     1501 cacgcagatc ctcgtaagcg tagaatagca cccacgtgga gccaggccgg cgaacatgcg
     1561 ataatccccc ttgagaacac agattcttgg ggggatctgc ttatgcccga tacgctaccg
     1621 cgcgatggcg gttacccggt gcgagttgcg ttggctcctg gacggattgt cattggagca
     1681 aaagaaggct catcaggctg taagcgccag gacagtgccg tgaaatcctt gtcattatca
     1741 taggcaatct cttccttcag ctgctagcca ctatacgagc taacttggcc tatgggagct
     1801 aggaattgtt cgctttcagg ttgtttgccc atagtcacca aaccgcactg caagctcgcg
     1861 tcccgctgaa aaccttgtgg agaacccaca cgtgatgcgc gtagcacaaa gaacgctggt
     1921 atgcgtggat aatcggtgat agtattgatt catcaagaaa gcgctggagg aaccactgaa
     1981 tgcagatagt cacagatcac ggagcagaca tcagccccga gcagcgagaa gggcttgata
     2041 tccactatgt tcctctcacg attacgctcg gagggcgagt gtaccggggc ggtgtggata
     2101 tcagtgttga agaattctat gaacttctcg agcgcaccgg agaaatgccc agcacgtcgc
     2161 agccgtctcc cggggatttc gccgagctat atcgaaatct ggcccgcacc gatccggata
     2221 tcctgtcggt ccacatatcg tcagggctta gcggcacagt gaacacggct cgaatgggag
     2281 cgagcatggt gcctgaagcg aatgttacca tttacgatac caagaccctg tctggggcgc
     2341 agggatggca tgtagaagcc gccgcgcgcg ccgctaaggc agggtggagc gttgaccgaa
     2401 tagtcggaat gctgcaacgg atgaccgctg ccaccgacac gctgtacaca ctgtctacct
     2461 tgaagtacct tatacacggc ggacgcatca gccatttgaa agggctggtc gcatcgatac
     2521 tcaatatcaa gcccataatc ggagtggaga aggtcggagg cacctatgtc actcgcggcc
     2581 agacccgcac actggaccgc gccattgaca ctattgtaaa catggtggca tcggtctacc
     2641 cggccaacac cgtaatgaga atgcaggtgc tgcatgggct gtgccctgaa ggcgcggtgc
     2701 gcctgcgcgc agggctcgac cgcatattcc aatgccgctg gctgcctgta gctccgatcg
     2761 ctccggtact cggcgcgcat accggcccgg gccttgttgg cgttgttttc gcgcccgagg
     2821 cgcagcttcc caagttctag ctgcctagtg atcgacgcct agcataaacc tgaccatatt
     2881 ggcggcaagc gccctcgccg gctcttcctg cagcgcgtcc cagctcagca ggtctgctcc
     2941 aacatacatg acgggaccat tcagcacgat ggcggcattt agtggcaacg ccctggatgg
     3001 tgccccggtc ctgtcagacc actggcctaa caccaccgaa cccggccatg gttttgtgac
     3061 catcccctcc ggcacgcgca gcctcacggg agcgccgacg ccttcccaga tcgcgcggat
     3121 cacagcgtct gagccattcg ccgcgtccac ttcaccctta cctatcggcg caatcgcgtc
     3181 gtgcatcggc gacacaccgg tccaccccgc taactccgcg ccaaacagat cagcaaggcc
     3241 aaacccgtat tcgccggcaa cggcctccgc ttccgtcttg agagaagtct ggtacgtggc
     3301 aagcaccctg cctccgactg ccacgaagtc cctcacagcc cgcatctggc gctccgacat
     3361 cctcctggcg ttggaaagca ccagcagagt cggcctccgg gacacagtgg cgaccaggct
     3421 ctgaagctcg tctgctggca caagcgcgca ttcgagcccg cccagcccga tgccgtctac
     3481 catctcctcg atcatgtcga ggtcgtcttc atagtaccag cccatgtcct cgtaccatgc
     3541 cttcacatcc tcgcttcgca taaccgcaac cagcggcccg catccgtccg gtgaatccgg
     3601 cagcggtcga atcgggccga gaacacgtga accgagagtc tcggcagtcg catcaaccgt
     3661 ggtcgccgcc aaccgagcct gtccttcctg tttgcggggc agttccagag tgactacgta
     3721 tggcaggtgg tcggaggcca ggctcctggg cacgcgggcg atgccgatct cgccagcgac
     3781 tccgatatcg ttcgaagcga atatgtagtc gatgcgggca ctgggatcga atacgcctat
     3841 tgtatagcct cccttgaggt actcgcgctc aaaaggcccc agcgccacca gccacctact
     3901 gtctaccaga acccggtgga gagcgtaggt atcgttatgc tctcgcaaca tcaaggccgt
     3961 ctccagtgca tgggtaggcg tcgcgttgaa gtcgcccatg atgatccggg gcgtcggtgt
     4021 gctcgacgcg atatcggcca atgcctcagc ctgtgccacc cgggatcccg agtccgcgac
     4081 ttcaaggtgg gtaacgaaca ccgtcacaat cgtgccgtcc acatccacct gcgcttccag
     4141 tgccgcccgc gcttgtgtac ggttggggtt gggcagctga tggacgcggt gagacagtat
     4201 gggaaagcgg ctaatgagag cattgcagaa gaagcctgtg gggcgggcga atatggggcc
     4261 gtaggcgtgg tgaaaaccga gcctgtcggc gatcagcttc gcctggtcat cgaaattaac
     4321 cttctgggtc ttctgctcta cctcgcacag gcctatgagg tcggcctgct ctgcacggat
     4381 caagtcggcg atgcggtcca agctatagac gaagtctacc cccagcccgg catggatgtt
     4441 gtaggtcatg accgtgatgc gctgccgcga tcgggcgctg cctattgcgg gcggcgtcgc
     4501 ctgggcatca gcacatgtgc acaggccgct aaggatcagc gcaatcccgg caatgactag
     4561 cacccgagca cggcggcgga ttgatcctga acacacatgc atggtggacg cctcccccat
     4621 tgtcgtctcc tgattaccat ttcctgagct gactctccgg ccataggcat gatataatat
     4681 ggaaaactgc tgagaggtgg acgccatgaa tccaactctg tttagagctg tggctgtgtc
     4741 aagggagctg aacctcacca caatcgcctt acagttcggc atcgagcgtc cgttttcctg
     4801 ggaggacacc ctggtgctct ccgaaaggca tctggatggc atcctgcccc agcctgcagg
     4861 taagggagtg tacctgttct ccttcgggac gctggtcttc gtcaactgcg ccgatagcga
     4921 tatcatggat gtcatagcct acctgaagaa gctcgaccgc aatcttgggg agtttgcgcg
     4981 agtggagtat gtagacgact acaagctgac tgtgtccggc gacgagctat cggtcaccta
     5041 cgacgagctc ataacgcctg agatgaaaga ctactaccga gaactggtgg cggtcattct
     5101 tgccaaatcc actgcgatgg agcgcataga agacgagatc agcaagctat tcgatgatgt
     5161 agagcagttc atcactcagc tcaaacgggg cacgctgtca ataagcgacg agcgcctagc
     5221 taggctctcg gggcggattc tgagctatcg atacagcagc atttcgtaca tcatgatcct
     5281 ggagaaaccc gatgtagtct ggcgcagcca ggaagcacag gaactctatt cggacctcga
     5341 gcgcctgttc gagctgccgg accgttacga gaagatctct cacaagactc agacgctgct
     5401 cgacatgacg caagtatttg cagatctgta ccatgccaag cgaggcactc gcctcgaggt
     5461 ggctgtcata ctgctgatcg ccttcgagat catcctcagt ctggtggagc ggttttggcc
     5521 gttctgatgt actccaatac cagcctgact acctcggcca ccctacccgg gtcaacctga
     5581 tcggcagtgt ctcccgggcc gtggtaaccg ccggcgaaat ccggctgaat caggttcacc
     5641 gcctccaccc cagcgtttgc gaaggacgaa tgatcgctgc cgctgctcat cgacttgcga
     5701 aacggaatgc ccaatttcga cgctaccctg cccaaatccc gcgccagctg agtatcgtgg
     5761 ccgctcgcgt tctcgatctg caagagaact ccacgcggcg atcccactgt gtcgatattc
     5821 aacatggccg cgtggccaag cggcaagcac ggttccgaca catagtgccg cgaaccgtac
     5881 atgaactgct cctcgccatt gaaagccacg aagactacgg tcagcgacgg acgaaccggg
     5941 ccgactgaga gcgccctcgc tatctcgata atcgcagcca cccctgaggc gttgtccatg
     6001 gcgccggggt tataggtacc gtccatgttg ctgcccacgt gatcaagatg ggcgccaatg
     6061 attatgtgat gtgcgccccc cgggagcact cctaccacgt tggctgtacg cactggctcg
     6121 atagagtagt ccattgacat gcgtattgta ccggaaggcg agtcggcggc tgaggttgca
     6181 gttgctattg cggtcggggt tgcggccggg gctgccccag accaagcagc aagctccgcg
     6241 aaagtcggtc tgtcgcaggt aaacaccatg ggctcgttgg cagtcgattc agccgtcggc
     6301 tgttggcccc cgtcgccctg atccgccgaa gccgcgctcg gagctatata cggcacaatc
     6361 gggaagtatc ccctgcctgc tgtgtcgatt tcaaggacga ccccgacaaa ctcggcttga
     6421 cctgcgccgg aagaagccgc gacgcgtttc atgatcgctt cgatctcggc tggattccct
     6481 gcgagatgca tcggaaccag cagtaccgcc tcgccgcgca ccctgtcgat gtcggcggcg
     6541 tgctgaagaa ctaccccagg ggccgtaacc tgtccgctga ccctgcagcc aggcgcagta
     6601 gatggggaga actgtgtaat gaacctgaac tgccgtgcga cactccccga cgcgcctacg
     6661 atctcaagcc tgggcgggct gtgcatgagc cttgtaggct ggatatacgg ctgcaaatac
     6721 ccgtcaagcc catcggggct tgcaagcccc agacgcctga agcagtcagc cacgtagttg
     6781 actgctgccc agtttccgtc ggttccggtc aggcgacccg cgtactttgg caagctaagc
     6841 tcgcaaatga tgcctgcgat gttcccaagg tcgacctcgt cggctcgctc tgccctgacc
     6901 ggcccgtcaa gcgccgagac tgccaacaat gcgaacacca ggccgaccag cgcatgccgg
     6961 caactcgtcc ggcctcgcct gcgcacggca aatatcgcca cccgatttac ctccagtcaa
     7021 gacttctgca gttcatctgg caaaggcgga acacaccgga aaataccgca aggttcacgg
     7081 tctgtcccat accctgtatg ttttcccaca ggccggacaa atccttgccc aacccgactt
     7141 tggcgacctg ggtcttgtcg cgagcggaag ccatacgtca ctgctaagtg gatcgtgtac
     7201 ccgcgttccg ggttgatgtt tcgacatccg cgtcatttcg cctgcaggcg atcggtgctc
     7261 agtgacggct tccgatcctg tcccataggg tccttgcctc ggccagctcg gccatggccc
     7321 ctgcccggtt ctgggccctt atgaacccct cggcccgagc tattgcggcc ctgagatcga
     7381 gtacgtcgtg gagttgggcc gagaactgca cacgcacagt caccctattg aacgcgtccg
     7441 ccagtttcct cgcgccctcc agcgcatctt gccactgttc gtccatgaca tgcgcctcta
     7501 cccgagatag ccatgccggc acatcatcgg cactccccag tgggtgcttg agaaccgcag
     7561 ccgacagcat caccgcgatg aacagagcga tcaagcccac cggcagtgca atgacaagaa
     7621 tgcgcttcag catggcgcag acctccagtc tcagtaaggt cccggataat cgcccgtgtc
     7681 caccgggttt tccacacgat cgtggtatgt gtctacatac aggttaccgg aggtgtccag
     7741 gacggccagg aagacgtctc gggagtcttg tatccctttc ttcctgagtt cagagtccag
     7801 ccacgaacgg tccaagtgca cctgcctcag gttctggtcg aatatcacgc cactgtatat
     7861 gagctcggta cctatgccct ggtactgtgt ggagatgctc atatcgctcc gcgtcacagg
     7921 ctggaattgg gtcttcttca acaccgacaa cgtcccgtta gtctcgagta ttgcgaactc
     7981 cacttcccca atgtcgaaca cgtccttcac acgcaactgc tccatgagat cggatgcacg
     8041 gtagcgcagc ttcctcatgg cgccttccat tatctttccg ttcattatca cgaccgtggg
     8101 ttcaccgtca atgtacttgg acgcgaacct ccactttagc gtgacccact gcatcgtgag
     8161 ccccgccgcc gtccacgcgg tcagcccaac ccagtgaggc cacgctgtcg atgataagtc
     8221 ggtggtgagc gcggcagcta tggacccgat agtaatgccg aggatgtagt cgaaatacgt
     8281 cagctggctn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna cttatggacg
     8401 gtgaagcaga gtgatccttc acgcagcctg gcttcctttc gctgacgccg ccgcaggggc
     8461 agtgcccacg gcaagtgcac tcgcagggaa tcaagccgcg ccggcgccgg gagggcgcct
     8521 tttcatatgg ggcgaatcgc ccgagcgttt tggcctgaag ctacgcggtc gcaagggccc
     8581 tttccccggg catccgtttc aggcttctgc cacagagctg atgcaggcat ttgctcttct
     8641 cgtggctgct gccggctgca gtctgaaccg caatcaaggc acagtagggt cggcggctgg
     8701 gcccgacgcg gtgcgcgcca gcgcaaagtt ggtgcagaag cccaggaaga gccatgccct
     8761 catactgctc cctacggatg atggttatcc cattccgtcg ccggctctgc ttaagcaggc
     8821 acggcgtgtt ctgccgactg cgcgcctcga gcggtggaca gccaacggcg gttcggttgg
     8881 cctacacaac gcaggttgct tccaagccac cggagtaagc atttcggcgc tcgatatcat
     8941 tcgtctgctc acggtattgc cgcagtcccg ccgggacgat ggaggcgact atcccgagcc
     9001 cgacgacaga ctcgtcagat taagactctc agccgctccc ggcagtgact ttgccttttg
     9061 gacacaggcg tccaaactgg ctcttgagct catggccaag cagaggttca tgcccgacat
     9121 ctgcgtctct acagacgact cacgagcgag agctgtatgg cgccccttgt gggaatccgc
     9181 ccaggacgtg tcgcgctttc gcgcgctggc cgacgccatg cccttgctgt gcagggctgt
     9241 gtggccgctg gaggagtctg gatcggcccg gatcgtgcct tcggacgtga tcttgcggag
     9301 tttcatcgat acttcgatgg atgagactgt caggggcatg ctggctatgg agcgcaacat
     9361 ccggtgggcc gattccattg ccaggcagga gtttgacgac gccgagcgcc ggttgctgga
     9421 gatggccatg ctcagcaaca aacgaccggc ctgcgcctgg atatgcagcc tcaacaagga
     9481 gaagcccgag atccggctgg aacaggcgcg cctcgactca gtccgccggg gcatcagcga
     9541 ttggatagcc gccgcgagct tgcgtcaggc gcctgcctcc agcgtgccct cggtttccgc
     9601 tgcgagcggt ttccgcacct gttttcgcct ggagccgccg aataccgagg ccgactcggc
     9661 ggaccatgag tgtgagaccg actccgccgc cgaattctgg acgctccact actacctcca
     9721 ggccgccgac gaccccagcg tactggtgcc ggcagctcgc gtatgggaag aaagtggcgg
     9781 tatgctgcgc ttcctgggca aacggttcga gaacccccac gagcgtctgc tggcagacct
     9841 cggtcaggcc gcgcgtcatt ttcccgcgat agaaacaagc ctcgacacag caacacctga
     9901 atcgtgcgag ctcactacga ccgaggccta ctcgttcctg cgggaggtct ccccgcagct
     9961 tgagcagctc ggcctgggag tactcgcccc gccctggtgg gaacgggtgt cgagcggccc
    10021 cgccagactc ggagtacgtc tcaaagtccg atccgaatct gcgcaagacg ccggcgccgg
    10081 tgaggaggcc aatgtcgcac ctcgcttctc cctagatagc ctcgtcgatt acgactggca
    10141 ggtagccctg ggcgatgagg tcatgagcaa ggacgaattc cgccggctcg ccgacctcaa
    10201 agtcccgctg gtgagggtga gaggcgagtg ggtcgagatc aatcccaggg aggtcgaatc
    10261 ggccatcgcc ttc
//
LOCUS       DAOTAC010000057        10171 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_5615_length_10171_cov_4.863681, whole genome shotgun sequence.
ACCESSION   DAOTAC010000057 DAOTAC010000000
VERSION     DAOTAC010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 10171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10171
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5615_length_10171_cov_4.863681"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="PLM74_03795"
     CDS             <1..778
                     /locus_tag="PLM74_03795"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HON42239.1"
                     /translation="GWPPGGFGRTQPGYKRTTFTFGNLSDADFSDFFKQFFGGFDIDG
                     GIFGQGVREHEFAQRRPARRDIEHEIEIGLEEAYRGGERSLELVDPDGARRRIAVTIP
                     AGIRNGSKLRVAGEGTGRTGSRGDLYLKVSIRPHPRFELRGNDLHTVVDVPVTVAALG
                     GEVAAPTLDGANVSLTIPAETQNGRVLRLRGLGMPSVRGESRGDLLVQVSLTMPRSLT
                     DRERELFRELASLRGDKVGAGPSADAGAATGAGENATRRR"
     gene            796..3411
                     /gene="clpB"
                     /locus_tag="PLM74_03800"
     CDS             796..3411
                     /gene="clpB"
                     /locus_tag="PLM74_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HON42240.1"
                     /translation="MRFDKLTVKSQEAIARAQSIARQRQHQGIGPAHLLAALLEQKEG
                     VVAPVLEKLDAAPEAVAREVAAALDKTPKVYGQEPGQYLTTPAANVLEDASSEAERLK
                     DEYVSTEHILLALAGSEDDTGKLLRSMGIKKDDIYKALVDVRGTQRVTDQNPEEKYQA
                     LERYSRDLTALAKRGSLDPVIGRDEEIRRVIQVLSRRTKNNPVLIGEPGVGKTAIAEG
                     LAQRIVAGDVPEGLKNKRVLALDLGSLIAGTKYRGEFEDRLKAVLKEIEEAAGEVILF
                     IDELHTLVGAGAAEGAMDASNMLKPALARGELKAVGATTIDEYRKHIEKDAALERRFQ
                     PVMVNEPTVEDTIAILRGLKERYEVHHGVRIKDSALVAAAVLSHRYISDRFLPDKAID
                     LVDEAASRLRMEIDSLPAEIDEVSRRIMQLEIERHALDKEDDDASRERLAKLDSELAE
                     LREKSDGMKAHWQREKDTISQIRQIKQKIEEAKHEEQNAERAGDLGRAAELRYGVQLT
                     LSRELDEANAKIAKLQTEKRFLKEEVEEEDIAEIVAKWTGIPVSRLVEGETQKLLRME
                     DLLRERVIGQDEAVRAVSNAVRRARSGLSDPARPIGSFLFLGPTGVGKTELGKALAQF
                     LFDDERAMIRIDMSEYQERHTVARLIGAPPGYVGYEEGGQLTEAVRRRPYTVILLDEI
                     EKAHQEVFNVLLQLLDDGRLTDGQGRTVDFRNTVVIMTSNLGSQYITEYAGRGDTSDG
                     GDHERGHDRMRDTIMDVLKQSFRPEFLNRIDEILIFNRLGRNEISRIVEIQLRLVRAR
                     LADKGFSIVWTPAVREYLARAGYDPIYGARPLKRAIQKELENALALEILQGRFKEGDV
                     IQVSVAEDGESLAFQ"
     gene            complement(3626..4060)
                     /locus_tag="PLM74_03805"
     CDS             complement(3626..4060)
                     /locus_tag="PLM74_03805"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HON42241.1"
                     /translation="MLSRHRIMERLDLISASVVRMQRFAGMSYGDFIADPDNAAIVES
                     YLRRSLEAVFDVGRHILAHTEGADLAGEYESIARGLGDLGVVDRAMSDTLVMMAGYRN
                     RLVHLYHLVSDRGLLDIVSENLPDLTRFVAQVDDYLTCAEHS"
     gene            complement(4050..4544)
                     /locus_tag="PLM74_03810"
     CDS             complement(4050..4544)
                     /locus_tag="PLM74_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HON42242.1"
                     /translation="MAIDGPGEVGALAAVCEAHGVTLCYLFGSQSENAWQILLGEQVA
                     INDPLADIDVGVVLAEFDSFREARALHRIYARLLSPLQDLFSPYTVDLVFLQEHHSVF
                     QMEAIKGRCAYSISNEYRFDYEEAIARRAADFRPVLDAYLEDVAQGFRERYRAQGGVV
                     RDAE"
     gene            4754..5077
                     /locus_tag="PLM74_03815"
     CDS             4754..5077
                     /locus_tag="PLM74_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42243.1"
                     /translation="MLTALNALRNIRAYGDDDVAQGRFDNLTLCEAHLLSDAVSHRTD
                     VALPEEVASATVLEKGFTAMELTLMLQIAGLKVENVWGGTAGNWGRRPLLMDEIELMV
                     VARLD"
     gene            5093..6145
                     /locus_tag="PLM74_03820"
     CDS             5093..6145
                     /locus_tag="PLM74_03820"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional class I SAM-dependent
                     methyltransferase/GNAT family N-acetyltransferase"
                     /protein_id="HON42244.1"
                     /translation="MIRDVIDLSQLTAPGLVLDVAGGGEGMIGHALGRQVVSIDISRS
                     ELEEVSNDALKLVMDAADMSFLDGSFTDATCFFGLMYMPTQLRPRVLAEVFRVLAPGG
                     RLRIWDAEIPSVEACGSHEDRDHRDEIFLVELEVMIDPGEHVRPHRTTYGVGWDDKAQ
                     TCCSITRAAVDCGFVVLGHAGSGRTFHLSLLKPYGTDGQLAGRTPTGVGPMRIVRAAA
                     RDLQDAYGVLADSCRRVVSRGRPVPHWLFAFEGQQHVADKIRDCDYFIGYIDDSPAGA
                     GIGAALLEWASSYIASRGRSIVRLECDAKSPGLCAYYESLGFQDRGLVSSANQLRRYE
                     RRYERRDTQQAPWYNR"
     gene            6176..6523
                     /locus_tag="PLM74_03825"
     CDS             6176..6523
                     /locus_tag="PLM74_03825"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HON42245.1"
                     /translation="MGYRMPDPAERAMELNSELNRIVGILKARNVDKVILFGSLADGR
                     VGSTSDIDLIIVDNTDKRFLDRLGDIYSAVQPRVATDLLVYTPDELERLIEESSFVRE
                     AVLRGRVVYEKDS"
     gene            6507..6914
                     /locus_tag="PLM74_03830"
     CDS             6507..6914
                     /locus_tag="PLM74_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003869055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HON42246.1"
                     /translation="MRRTPSDEAGRWFLQAQRDLDDARYSAAGQRFNLACFLAQQSAE
                     KALKALLYFAGAEAVWGHSVAELCQDIIAIHPDLAPLAAIGGPLDKYYIPTRYPNGLP
                     GGIPSDAFVSADAQMAISMAEQVLDTISSKLTS"
     gene            complement(7099..7605)
                     /locus_tag="PLM74_03835"
     CDS             complement(7099..7605)
                     /locus_tag="PLM74_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42247.1"
                     /translation="MRPENYLVVQARIAKERKNLDRLLNQMSRHGIYPEVHAESMGGF
                     RLCDNEALRIIGSMLHDFYTFVENIAKTVAARVDGGIPDGDDWPRELLEQMSLPIPGL
                     RPPLLSGDTVELLNKFRAFRHVFRNIYGFQLDAGRTLELLRDLNRTVVALNTDLDRFL
                     SEIDRAVL"
     gene            complement(7602..7964)
                     /locus_tag="PLM74_03840"
     CDS             complement(7602..7964)
                     /locus_tag="PLM74_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HON42248.1"
                     /translation="MTDDGIRGWDVPEIREAVFRRSKQRADLVRADAMERARRIANLL
                     KVRYSASAVTLFGSLAETCFHDKSGIDILVEGFRGQYWKMYVIADEIAGPFDLSIVCI
                     EDAQDTLIEHAREGGICL"
     gene            complement(8146..9891)
                     /locus_tag="PLM74_03845"
     CDS             complement(8146..9891)
                     /locus_tag="PLM74_03845"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON42249.1"
                     /translation="MSPTKEEGFLQIARKALPLVCRVSPGLFVFEYAVTVLDALLLVL
                     TTVALRDFFEHVTGLTAGSVSFDSTLMSLLLFALLKVADEVIDGVANFIGEHYAGKSS
                     QVMMNELNLKADRLDALDFEDPQILNQMDQAYRGAYNVRNLVHVLMDIACLYIPYFVF
                     MGVFLRRAEPMLPLILLLVFVPVLASHFLRSSYYTRLDDRTVALRRKEKHYKECLTAR
                     AFFKETRALGAGAFFLSRYIQAFKNAQELRYQTELGSQGVAIAASGLALAGYTAILGI
                     LVSLVIRGRITIGSFSAILAATLEMFAMMTEVLDNRLGELSAGLGQIRKYFRFLDLPE
                     QEYGRIHVDDPKDIVLEGVTFTYPGAARPALSGISLRITAGETLAVVGENGSGKTTLA
                     KLILGLYRPDKGRILVGDVPLRELASSSAYKNASCVFQDFQRYQLSLRDNVAISDFIK
                     LSASSDDEIQDALAAAGFRADDQQMTQGLDTLISKEFGGTDLSRGEWQRLSIARAYYR
                     SHQLIVLDEPTAAIDPFEEDRIYRDFMRLKKNHTAVIITHRLGAVRLADRVLVLKEGR
                     IADIGTHDRLLDESQ"
     gene            complement(9888..>10171)
                     /locus_tag="PLM74_03850"
     CDS             complement(9888..>10171)
                     /locus_tag="PLM74_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42250.1"
                     /translation="LLVLDEPAAALDALAESRIYRIIRDIGARRCTTTLYVTHRLAAA
                     RSADEIILMKDGRIIEQGTHAALLARDSYYAQMFNTQRKWYLRGQVAKA"
BASE COUNT         2201 a         3000 c         3092 g         1878 t
ORIGIN      
        1 cgggtggcct ccgggcgggt tcgggcgcac ccaaccggga tacaagcgaa ccacgttcac
       61 cttcggcaac ctgtcggacg ccgactttag cgacttcttc aaacagttct tcggagggtt
      121 cgatatcgac ggcggcatct tcgggcaagg ggttcgcgaa catgagttcg cccagcggcg
      181 ccctgcgcgg cgcgacattg aacacgagat cgagatcggt cttgaggaag cgtaccgtgg
      241 cggcgagcgt tcgctcgaac tggtggatcc ggacggggcc aggcggagga tcgcggttac
      301 cataccggca ggcatccgaa acggaagcaa gctgcgcgtg gcaggcgagg gtacaggcag
      361 aaccggatcg cggggcgatc tctacctgaa ggtgagtatc cgtcctcacc cacgtttcga
      421 attgcgcggc aacgaccttc acaccgtggt agacgttccg gtaaccgtcg cggcgctggg
      481 cggcgaggtt gctgcgccca cgctggacgg cgcgaatgtg tcgctcacca tcccggccga
      541 aacccaaaat ggccgagtat tgaggcttcg cggcctcggc atgccgtccg tgcgcggaga
      601 aagccggggc gacctgcttg tgcaggtgag ccttacaatg ccgaggagct tgaccgaccg
      661 cgagcgagag ctctttcggg agcttgcctc gctgcgaggg gataaggtcg gtgccgggcc
      721 gagcgccgac gcgggcgccg cgacaggcgc cggcgaaaac gcaaccagac gcagataaca
      781 aggagggttg gctacatgag attcgacaaa ctcacagtca agtcacagga ggcaatcgcc
      841 cgcgcgcaga gcatcgcgcg ccagcgacag catcaaggca tagggcccgc tcatctcctc
      901 gcggcactcc ttgaacagaa ggaaggcgtg gtagcccccg tccttgaaaa gctggacgcg
      961 gcgcctgagg ccgtggcccg agaggtcgcg gcggcgctcg acaaaacccc gaaggtctac
     1021 gggcaggaac ccgggcagta tctcaccaca cctgcggcga acgtgctgga agatgcgtca
     1081 tccgaagctg agcgtctcaa ggacgagtat gtaagcactg agcacatcct tcttgccctg
     1141 gccggcagcg aagacgatac cggcaagcta ctgcggtcga tgggcatcaa gaaggacgac
     1201 atatacaaag ccctggtgga cgtgcgcggc actcagcgag tgaccgacca gaacccggag
     1261 gagaaatacc aggcgcttga aagatacagc cgcgacctga ccgcgctcgc gaagcgcggt
     1321 tcgctcgacc cagtgatcgg cagagatgaa gagatccgcc gcgtgatcca ggtgctgtcg
     1381 aggcgcacca agaacaaccc cgtcctcata ggcgaacccg gcgtaggcaa gaccgccatc
     1441 gccgaagggc tggcccagcg aattgtggca ggcgacgtgc ctgagggcct gaaaaacaag
     1501 cgagtgctgg ctctggacct tggatcactc atcgcgggga cgaagtacag aggcgaattc
     1561 gaggacaggc tgaaggccgt actcaaggaa attgaggagg ccgcaggtga ggtcatcctg
     1621 ttcatcgacg agctacacac cctggtgggc gcgggcgcgg ccgaaggcgc tatggacgcc
     1681 tccaacatgc tcaagcccgc attggccaga ggcgagctta aggcagtggg cgcaaccacc
     1741 atcgatgagt acaggaagca tattgagaaa gacgcggctt tggagcgcag gttccagcct
     1801 gtcatggtca acgagcccac ggtggaagac accatcgcca tcctgcgcgg gctcaaggag
     1861 aggtacgaag tccatcacgg agttcggatc aaggactcgg cgctggtggc ggcggcagtg
     1921 ctgtcgcacc gctacatctc cgaccggttc ctgccggata aggccatcga cctagtggac
     1981 gaggcggcat ctcgcctcag gatggaaatc gactcactcc cggccgagat agacgaggtg
     2041 agcaggcgga tcatgcagct cgagatagag cggcatgcgc tggacaagga agatgacgac
     2101 gcctcccgcg aaaggctcgc gaaactagac tcagagcttg ccgagttgcg tgagaagtcg
     2161 gacggtatga aggctcactg gcagcgggag aaggatacga tatctcagat ccgccagatc
     2221 aagcagaaga tcgaagaggc caaacatgaa gagcagaacg ccgagcgcgc cggcgacctg
     2281 gggcgcgcgg ctgagctcag gtatggagtc cagctcacgc tctcccgcga gcttgacgag
     2341 gcaaacgcca agatcgccaa gctgcagacg gaaaagcgct tcctcaagga agaggtcgaa
     2401 gaagaggaca tcgcggaaat cgtggccaag tggaccggca ttccggtgtc gcggcttgta
     2461 gaaggtgaga cccagaagct cctgcgcatg gaagacctcc tgcgcgagcg cgtgatcggc
     2521 caggacgagg cggtgcgcgc agtgtccaac gctgtgcggc gcgcgaggtc ggggctttct
     2581 gacccggcca ggcccatagg ttcgttcctg ttcctcgggc ccactggcgt aggcaagacg
     2641 gagcttggga aggccctggc ccagttcctg ttcgacgatg aacgcgccat gatccgaatc
     2701 gacatgtcgg aatatcagga gcggcacacc gtagcccgcc taatcggggc ccctcccgga
     2761 tacgtgggct acgaagaagg cggccagctg accgaggctg taaggcgccg gccctacaca
     2821 gtaatcctgc tggacgaaat cgagaaggcg catcaggaag tcttcaacgt gctgctgcag
     2881 ctgctcgacg acggcaggct gaccgacggg caaggacgga cagtcgactt ccgaaacact
     2941 gttgtgataa tgacgtccaa cctgggaagc cagtatatca cggaatacgc cggtcgcggc
     3001 gatactagcg atggcggcga ccatgagcgc ggccacgacc ggatgcgcga cacgataatg
     3061 gacgtgctca agcagtcgtt caggccggag ttcctgaaca ggatagacga gatcctgatc
     3121 ttcaaccgtc ttggcagaaa cgagatatcc cggatcgtcg agatccagct taggttggtg
     3181 cgcgcgcgcc tggccgacaa gggtttctcc atagtatgga cccccgcggt ccgcgaatac
     3241 ctggctcgcg ccggttacga cccgatctac ggcgccagac cgcttaagcg tgccattcag
     3301 aaggaactcg agaacgccct cgcactcgag atactccagg gcaggttcaa agagggcgac
     3361 gtaatacagg tgagcgtggc cgaggatggc gagtcgttgg cgttccagta ggccattgtg
     3421 gcacgcattc cggcaaacga tgttgcgcgg caatgcccgg actggtatgg tcgtatcacc
     3481 gacacgcaat cgaggtgact gacccatgac ccgcactaga gtcaggctgt ccggtaaaca
     3541 ccaggtcgtg attgccaagc cggaatcacg cacgtgcaga ttcgccggcg cgcgttctgc
     3601 atcttccccc gcagacaggc gacgcctacg agtgctcggc gcacgtcaga taatcgtcga
     3661 cctgggccac gaatcgagtg agatccggca gattctcgga tacgatgtcg agaagtcctc
     3721 tgtcggatac caggtggtac aggtggacga gacggtttcg atacccggcc atcatcacca
     3781 gagtatcgga catggccctg tctactaccc caaggtcgcc caggccacgg gctattgact
     3841 cgtactcgcc ggcaaggtcg gcgccttctg tgtgggccag aatgtgccgt cccacgtcga
     3901 agacggcttc cagcgaacgc cgaaggtagc tctcgacaat ggcggcattg tccgggtcgg
     3961 caatgaaatc gccatatgac attccggcga atcgctgcat gcggacaacg ctcgcgctta
     4021 tcagatcgag tcgctccatt atcctgtgtc tactcagcat cccgcaccac ccctccctga
     4081 gccctgtagc gctccctgaa cccttgggca acgtcctcaa gatacgcgtc aagcacggga
     4141 cgaaaatcgg cggccctgcg ggcgatcgcc tcttcgtagt cgaaccgata ctcattgctg
     4201 atcgaatacg cgcacctacc cttgatggcc tccatctgga acaccgaatg gtgctcctgc
     4261 agaaacacca ggtcgacggt gtacggagag aacaggtcct gaagcggcga caagagccgg
     4321 gcgtagattc ggtgaagcgc tctcgcctcc cggaatgagt cgaattcggc caacacgacg
     4381 cccacatcta tgtcggccag cggatcgttg atggcgacct gctcgccgag aaggatctgc
     4441 caggcgtttt ccgactggga gccgaacagg tagcatagag taacgccgtg tgcctcacag
     4501 acggccgcca aagcgccgac ttcaccaggg ccatcgatag ccaacgccca tcacatcctc
     4561 tctccatcca gtatcgccac agtcggcacg acagatgagt tctgtccatc tcacccactc
     4621 tcctgcaggc ggtcgcgagg cagtggcgca ggaaccagac cacccgcgga gaatcacgta
     4681 cgggcacact gatgatagga ggtgtgcccc ataacttgga agggtttctt cgatggtccc
     4741 gggaggcagg ttcgtgctca cagcgctcaa cgcgttgaga aatatccgcg cctacggtga
     4801 tgacgacgtc gcccagggca gattcgacaa cctcactctg tgtgaggcac acttgctcag
     4861 cgacgcggtg agccaccgga cggatgttgc ccttccggag gaggtagctt ctgccaccgt
     4921 cctggagaag ggattcaccg cgatggagct gacattgatg ctgcagatcg ccgggctcaa
     4981 agtcgagaat gtgtggggcg gcacggccgg caactggggc agaaggccgc ttctgatgga
     5041 cgagatcgag ctgatggtcg tagccaggct ggactgagga ggtcttcctt gcatgatcag
     5101 ggatgtaatc gacctgtcgc agctcacggc ccccggcctg gtgctagacg tagcaggggg
     5161 cggcgaaggg atgatcggtc acgcgctggg cagacaagtg gtctcgatag atatctcccg
     5221 gtctgaactg gaggaagtat caaacgatgc gctcaagctg gtgatggacg cagcggacat
     5281 gtccttcctt gatggctcct tcaccgacgc gacttgtttc ttcgggctga tgtacatgcc
     5341 aacccagctc cgcccgcgag tgctggcaga ggtctttaga gtgctggccc ctggtggacg
     5401 cctgcgcata tgggatgccg agatcccgtc ggttgaggcg tgcggcagtc acgaggaccg
     5461 cgaccaccgt gacgagatat tcttggtcga acttgaggtc atgatcgacc caggggagca
     5521 cgtacgcccg cacagaacaa cctatggagt cggatgggac gataaggcac aaacctgctg
     5581 cagcattacc cgggcagcgg tcgactgcgg ctttgtggtg ctgggccatg cgggctcggg
     5641 tcgcactttc cacctgagtc tgctcaagcc ttacggcact gacgggcagt tggccggccg
     5701 tacgccgaca ggagtcgggc caatgcgcat tgtgcgtgcc gctgctcgag atctgcaaga
     5761 tgcctatgga gtgctcgccg acagttgccg acgagtcgtg agtcggggca ggcccgtccc
     5821 ccactggctg tttgccttcg agggccagca gcatgtagcc gacaagatac gcgactgcga
     5881 ctacttcatc ggctatatcg acgattcgcc cgccggcgcg ggcatcggcg cagcattgct
     5941 tgagtgggcc tcttcataca tagcttcgcg cggcagaagc atcgtgaggc tggagtgcga
     6001 cgcgaaaagc ccggggctct gcgcctacta cgaaagcctc ggatttcagg atcgcggcct
     6061 ggtttcctct gccaatcaac tgcgccgtta cgagcgccgt tacgagcgcc gggacacgca
     6121 acaagcgcca tggtataatc gctagtgaga gagtgtattc ctgggaggca ggctcatggg
     6181 ctatagaatg cccgacccgg ctgaaagggc tatggagctc aatagcgagt tgaaccgaat
     6241 tgtgggtatt ctgaaggccc ggaatgtcga caaggtgatt ctgttcggct cgctagcgga
     6301 cggtcgcgtc ggctctacca gcgatataga cctgatcatc gtggacaaca ccgacaagcg
     6361 cttcctagat agattgggcg atatctactc cgcagtccag cccagggtgg ccactgacct
     6421 acttgtgtac actcccgacg aactggagcg cctaatagag gagagctcat tcgtgcgcga
     6481 ggccgtgctt aggggaaggg ttgtatatga gaaggactcc tagcgacgaa gcaggcagat
     6541 ggttccttca ggcccaacga gacctggacg atgcccggta ctcagctgcc ggtcagcgct
     6601 tcaacctggc gtgttttctc gcacagcaat cggccgagaa agccctaaag gcactgctct
     6661 actttgcagg cgcagaggct gtgtgggggc actcggttgc cgagttatgc caggacatca
     6721 ttgccattca tcctgatctt gcgccgctgg ccgcgatagg gggcccgctg gacaagtact
     6781 acatccctac aagatatccc aatggcctgc cgggagggat accgtcagac gcgtttgtgt
     6841 cggcggacgc tcagatggcg atctcgatgg cagagcaagt cctcgatact atctcgtcga
     6901 agctgacctc ttgagctcaa cgggaccgag atgtcaactg tgaggctgga gtgcgccgcg
     6961 aaaggcccgg ggctttgcgc cgattacaaa agcctcgggt ttcaggatcg cggcctagtg
     7021 tcgcccgccg atcagctgcg ctgttgcagc tgtgaaggct gatgagggca gaacaggcat
     7081 ctgccgcccc ggcttttctc acagtactgc ccgatcgatt tcggacagga accgatccag
     7141 gtcggtattg agcgccacca ccgtcctgtt taggtctctc agcaattcca gagtccgccc
     7201 tgcatccaac tgaaatccgt agatgttcct gaaaacgtgc ctgaatgcgc ggaacttgtt
     7261 caggagctct accgtgtcgc cggacagcag cggcggacgc aatccaggga taggcagaga
     7321 catctgctcg agcagctctc tcggccagtc gtcgccgtca ggaatacctc catcaactct
     7381 cgcagctacg gtcttggcga tgttctctac aaacgtatag aaatcatgaa gcattgatcc
     7441 gattatgcgc agtgcctcgt tgtcgcacaa ccgaaaaccg cccatactct ctgcatgcac
     7501 ctcagggtag atgccatgcc tcgacatctg gttgagaagg cgatcgaggt tctttcgctc
     7561 cttggctatt cgggcttgga ccactaggta gttctcgggc ctcaaaggca aatcccccct
     7621 tcccgcgcgt gctcaatcag cgtgtcctgc gcgtcttcga tacacacgat gctgaggtcg
     7681 aaagggccgg cgatctcatc cgctatgaca tacattttcc agtattgacc cctgaaccct
     7741 tcaacgagaa tatctatgcc tgacttgtcg tggaagcagg tttcagccag ggagccaaat
     7801 agcgtgaccg ctgaggccga atagcggacc ttcagaagat tggctatgcg acgcgctctc
     7861 tccatcgcgt cagccctgac aaggtcagca cgctgcttcg atcgtcgaaa caccgcctct
     7921 cggatctcag gtacgtccca cccgcggatc ccatcatcag tcacggtcac accccctccc
     7981 ggaaaaggcc aagccctaca ggctgccgat gcgctgtgct cacgcccaca cagccagatc
     8041 cacgatcaca caagagatta ttcccccacc atcaaggcga ctccttcatc gatacacacc
     8101 tttctactgc aggtacctgc tctgggccgt ccagagtcta cggtattatt gcgactcgtc
     8161 taggagtcgg tcgtgggttc cgatgtctgc tattcggcct tccttgagca ccagaacgcg
     8221 atcggccagg cgtactgcgc ccaggcggtg cgtgatgatg acggcagtgt ggttcttctt
     8281 cagtctcatg aagtcgcggt agatgcggtc ttcctcaaag ggatcgatgg cggccgtcgg
     8341 ctcgtctagc acaataagct ggtgagacct atagtaggcc cgggcaatgg acaggcgctg
     8401 ccattcgccg cgcgacaggt cggttcctcc gaattccttg ctgatcagcg tatccaggcc
     8461 ctgcgtcatc tgttggtcgt cggcgcgaaa cccggccgcc gccagggcat cctggatctc
     8521 gtcatcgctt gaggcgctca gcttgatgaa gtcgctgatg gcgacattat ccctcagcga
     8581 tagctggtaa cgctggaaat cctgaaacac gcaggaggcg ttcttgtagg cgctgcttga
     8641 ggccaactcc ctcaagggaa catcgcccac caagatccgg cccttgtcgg gccggtacaa
     8701 gcccagaatc agtttggcca gcgtcgtctt ccccgaaccg ttttcgccga cgacggcaag
     8761 cgtttctccg gcggttatgc gaagggagat gccactaagg gccggtcgcg cagcgccggg
     8821 ataagtgaag gtgactcctt cgagcacgat atctttcgga tcgtcgacgt gtatccgccc
     8881 atactcctgc tccggcaggt cgagaaacct gaagtacttg cggatctgtc ccaggccggc
     8941 agacaactcg cccagcctgt tgtccaagac ctctgtcatc atggcaaaca tctcaagcgt
     9001 cgccgccagg atggccgaga acgagccgat ggtaatcctg cctcgtataa caagcgaaac
     9061 gagaattccc agaatagccg tatatcctgc cagggcaagc ccgctcgcgg cgatcgctac
     9121 gccctggctc ccaagttcag tctggtaacg cagctcttgg gcattcttga aagcctggat
     9181 gtaccgggac aggaaaaacg ctccggcgcc cagcgccctg gtttccttga agaacgctct
     9241 ggccgtcaga cattctttgt agtgcttctc cttgcggcgc agcgcgacag tgcgatcatc
     9301 gagccgcgta tagtagctgg atcgcagaaa atgggaggca agcacgggaa cgaagaccag
     9361 cagcaatatc aagggaagca ttggctcggc acgtcgcagg aagactccca tgaacacgaa
     9421 atacggaata tataggcagg cgatgtccat caggacatgg acaagattgc gtacattata
     9481 ggctccgcga taggcctggt ccatctggtt cagaatctga gggtcctcaa agtccagcgc
     9541 gtccagcctg tctgccttca ggttcaactc gttcatcatc acctggctgc tcttgccggc
     9601 gtaatgctca ccgatgaaat tggcgactcc gtcgatcacc tcatctgcca ccttcagcaa
     9661 ggcaaacagg agcagcgaca tcaaggtcga gtcaaacgaa accgagccgg ccgtgagccc
     9721 ggtcacgtgt tcgaagaaat cccgaagagc cacagtcgtc aacacgagca acagtgcatc
     9781 cagaacagtg actgcgtatt cgaagacaaa aaggccgggc gatacccggc aaaccaatgg
     9841 cagcgctttg cgggcaatct gcagaaagcc ctcttctttt gtgggactca tgctttggcc
     9901 acctgccctc tcaggtacca cttccgctgt gtattgaaca tctgggcata gtaggagtcc
     9961 ctggcgagca gggcggcgtg cgtgccctgc tcgatgatcc ggccgtcctt catcaggatg
    10021 atctcatcgg cggatctggc cgcggccaag cggtgggtaa cgtacaaggt agtcgtacac
    10081 cgtctggcgc cgatgtcgcg gataatgcga tagatccggc tctcagccag agcgtcaagg
    10141 gccgccgcag gttcgtccag caccaagagc t
//
LOCUS       DAOTAC010000058        10108 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_5661_length_10108_cov_4.216453, whole genome shotgun sequence.
ACCESSION   DAOTAC010000058 DAOTAC010000000
VERSION     DAOTAC010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 10108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10108
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5661_length_10108_cov_4.216453"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="PLM74_03855"
     CDS             <1..266
                     /locus_tag="PLM74_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON42251.1"
                     /translation="VVELFDNPIHPYTSGLLTSRVRREYKGRDLPYIEGYVPRAYEFP
                     EGCRFHPRCPRAMAKCREIPPPEFEPASGHTVACWLLEEGDDR"
     gene            263..1252
                     /locus_tag="PLM74_03860"
     CDS             263..1252
                     /locus_tag="PLM74_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HON42252.1"
                     /translation="MKIVSTRNLKKYFPIRSGVFLQVSGWVRALENVNMEISQGETIG
                     IVGESGCGKSTLGRAIVKIYEPTAGSITYCDPSGASHDISAKIDRRLEPVFRKDVQMI
                     FQNPFDSLDPRMTIRDTIREPLHTHRLLASKKEMDGRVAELLMKVGLYPEYAQRYPHE
                     FSGGQRQRIAVARSISVNPRLLICDEPTSALDVSVQSQIINLLKDIQRDTNMALIFIS
                     HNLDVVHHMSDRIMVMYLGNVVESAPATELFDHPAHPYTQALMSAIPSWNPKDRKLGL
                     VKLEGEPPSPINPPSGCPFHPRCRHRLAACSKVKPEAAEVSKGHFSACHLHGV"
     gene            1841..2611
                     /locus_tag="PLM74_03865"
     CDS             1841..2611
                     /locus_tag="PLM74_03865"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009218281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HON42253.1"
                     /translation="MGRARKLRINRGKRLAIQVGGRAYARIPIKTHVVTAADDIVDVV
                     QRYAGPLLHPRDVLVISEKVVSITQQRAYPISQIKPSCLARILSKFVHKSPYGIGLGS
                     PWTMELAIGEASPLRVVTAAAMAGVAKVFGARGVFYRVCGSGVAAIDGPCDYTLPPYN
                     RYAILGPSKPSAVAESASRVAGVPVAIVDANDLGVTVLGASSGCLERVFLAEVLADNP
                     LGQSCEQTPIGIVRPARYVPVRGGAVTLRRSQEGWRRC"
     gene            2605..3573
                     /locus_tag="PLM74_03870"
     CDS             2605..3573
                     /locus_tag="PLM74_03870"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HON42254.1"
                     /translation="MLRRGTGHTSGRRRGGRVAIEAALVFMVVVALAGAAWRTRGRMG
                     SDIALGSAVSAIVVEVESGDVLYEQGAERRIPPASLAKLMTVLLAVEAVEGGLASMLD
                     VVTVSRHAASMGGSQVWLGAGERYTLGQLLESVMIASANDSAVAVAEHLAGSERQFVG
                     LMTKRASELGMCSTRFVNATGLPAGEGEHDGYTTASDMARLAREALGHQVILEWSSTR
                     SKAFRNHPLFIMSNTNPLLGMYPGCDGLKTGHTKAAGYHLIATARADGVRLVAVIMHA
                     DNEEARATGCARLLDYGFAALRAGATARSTTETGVRKPGVRDPGVR"
     gene            3674..4072
                     /locus_tag="PLM74_03875"
     CDS             3674..4072
                     /locus_tag="PLM74_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HON42255.1"
                     /translation="MELPPLGDQELELLRFITEHAPITVREAVDSYGKDRGLARTTIL
                     TMMERLRAKGYLIRSEQGTANEYSPRLSRSELMRGVVRGFVEKTLGGTLSPFVAYLTQ
                     DAAVSRDELEDLKRLVEQLESREGRGRGRQ"
     gene            4069..5223
                     /locus_tag="PLM74_03880"
     CDS             4069..5223
                     /locus_tag="PLM74_03880"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HON42256.1"
                     /translation="MSAVLYDLLLVVMPQRWAGSMWGALWQGSAALAVVWIICRAFPR
                     IPANTRCWLWRLGLMKPFLQLLAAPQVDLAWLPRARTVLASVVSQRFAADLPTRCALL
                     IEASRPMMALWIAGVAAVAASILAALARSRSIAERCAPMRDDELDEVVAELCRRIGIS
                     ARPDTVVADWASVPMILRRPRGHVMVVPSDLLISARHEDLRLALAHELAHIKRKDLAW
                     NWLPAAAKALFFVNPLVWVAGRELGIAQEMACDEMAVRASGAGFARYGKLLLTMVKPR
                     RRAEPTWAVATVSAGASAKVMRRRLIELGHAQDPPALRLAGSMVALLIVCALVMPFRI
                     VEAPRNEGQWPGVSMIRTRELLVGSLERTAMAIIVERRVAESRASLPQHP"
     gene            complement(5284..6240)
                     /locus_tag="PLM74_03885"
     CDS             complement(5284..6240)
                     /locus_tag="PLM74_03885"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HON42257.1"
                     /translation="MATPSFRPYQTEDDYWRMREFLRLVFMANNGLERSWHVARLDYA
                     RWHLCLNCDKVRLEDIVYLWETDCDDGQEREIAAIVMPEGGPGEAHLMVHPDYHTAEL
                     EEEMLSIAEERLASTGPDVVRKLCVWSPEQDSVRQSVLSKRGYEKRSAAEHQWRRDLG
                     QPIPDAPIAPGYTIRSLGDGLELLERCYASGLGFHEGDINTAVENRDDPTWYRNIQNA
                     PLYRRDLDLVAVAPDGAIGAFCTIWFDDVTRSAYVEPLATVPAHRRLGLGRALLTEGL
                     RRLRCMGATRAFVGGFSAAANALYGSVMGPKYELYEPWVKKW"
     gene            complement(6247..7872)
                     /locus_tag="PLM74_03890"
     CDS             complement(6247..7872)
                     /locus_tag="PLM74_03890"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008241 - peptidyl-dipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HON42258.1"
                     /translation="MTTDIRAFVDDRVESISNLAKVINLATWNADTTGDSGQRRVATE
                     ARKRLAQLLSDPGDYRILREAQQAQSETVGTPDGVDPLLAREVSVLARMYARNQMTPE
                     EIAEQTDLQAEITDIFVHFRADLEGQPASENDINQVLAEERDNLRRLKAWEASKQIGR
                     EAAPKVVELVEARNRAARRMGYRNYYSMSLANDELDETWLFTLVDSLAAKTDAVFASE
                     KARLDRSLAAKFGVEPEQIGPWHYSDPFFQRAPADGSVDLDPVFCGKDLPALARRFYD
                     GIGMEVDDILSRSDLYERPGKYQHAYCEDMDREGDIRVICNLRDNHDWMSTLLHELGH
                     GVYFNYIDPGLPYLLREHAHLLTTEAVAMVMGDQTYDARWLTEIAGVEPARARELEVA
                     AARVSALESLIFIRWCLVMVNFERALYASPRRNLNTLWGQMVSRYQLVQPPAGRDEPD
                     WAAKIHMALYPVYYQNYMLGQLLSVQFQSAIARRFGTKSLTGMPEAGRWLIDSVLGPG
                     AKWHWNEMIQRATGSGLSDQPFVNDIAVLNEKE"
     gene            complement(7974..9383)
                     /locus_tag="PLM74_03895"
     CDS             complement(7974..9383)
                     /locus_tag="PLM74_03895"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HON42259.1"
                     /translation="MRTCNIPWHARKPKYSRATVAWLRLAAVCVGLALLVGSAHAAAP
                     LASASRILVIAPHPDDETLGAGGLIYSACRRGANVKTVFMTMGDGSAVAAGMLFGTAR
                     PSLHDMVALGLRRRNEAVAALGVLGVDEDDIVFLGYPDGGLADIIDNRHWGPAKPYTS
                     KHTGHAGNPYVSSFTPDAQYCASRIVDDLATILAEYMPDMVLVPHPNDTHSDHWATYA
                     LTMYTIEQLSYVELTGASVLAYMLHTGPDWPQPWGYRPDLPLDPPRGRDSPPTAWISA
                     ELTEDAIAAKLKALEKYRSQLSGLSAFLKSFIRTNESFCSVPVLDLAPPQVFGRTHKN
                     DAPSTRPDPSPFRVLPPSGEIRSVVANRTDDGVAVTAVLAWRPSPSVTYRLQIVSCDP
                     APELSGLLYGQRLRPTAKELTYETTPSSGQRRPWEAVFLIPQHDLESLGPKALIEVVT
                     IRRGLVLDRSGWILLRLCQ"
     gene            complement(9507..>10108)
                     /locus_tag="PLM74_03900"
     CDS             complement(9507..>10108)
                     /locus_tag="PLM74_03900"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HON42260.1"
                     /translation="VIRASIEEFAKGYEVASTNEIVRKAGISKGLLFHYFGSKKNLYM
                     TVFREVVQRTTETLLSRAGELPGDVIERMLRLTLIKLELYREDPKGWHFLADAVSNPP
                     HELKQEIRSVQAELTDFGMKAFLTGLDLAQLRPDVDAECLLRFVLLFLKGLEQEYLGR
                     GDLSSLDWEQIIKEFMVYVELLRTGIGSQCGSDRDPHMG"
BASE COUNT         1981 a         2882 c         3181 g         2064 t
ORIGIN      
        1 cagtagtgga gctgtttgac aatcctatcc atccgtatac gtccggactg ctgacctcgc
       61 gcgtgaggcg agagtacaag gggagagacc tgccgtatat cgaaggttac gttccgagag
      121 cctacgagtt cccggaaggc tgcagattcc atcccaggtg cccgagggcc atggcgaaat
      181 gcagggagat cccgcctccc gagtttgagc cggcgtccgg ccacaccgtt gcctgctggc
      241 tgctggagga gggcgatgat agatgaagat tgtgtctacc agaaacctca agaagtattt
      301 cccgataagg tcgggggtgt tcttgcaggt cagcggctgg gtgagggcgc tggagaacgt
      361 caacatggag atcagtcaag gcgagacgat cgggatcgtc ggcgagtcgg gctgcggcaa
      421 gagcacgctc ggaagggcga ttgtgaagat ctacgagccc acggccggca gcatcacgta
      481 ttgcgaccca agcggggcca gtcacgatat ctccgcgaag atcgatagac gactggagcc
      541 ggtattccga aaagacgtgc agatgatctt ccagaacccg ttcgactcgc tcgaccccag
      601 aatgactatt cgcgacacaa tacgtgaacc cctccacact cataggctgc ttgccagcaa
      661 gaaggagatg gacgggcgcg tagcggagct actgatgaaa gtggggctgt accccgaata
      721 cgcgcagaga tatccgcacg agttctccgg cgggcagcga cagagaatcg ccgtagcccg
      781 ttcgatatcg gtcaacccaa ggcttctgat atgcgacgag cccacttcgg ccctggacgt
      841 atcggttcag agccagatca tcaacttgct caaagacata cagcgcgaca ctaacatggc
      901 actcatattc atctcccata acctcgacgt ggtgcatcac atgagcgata ggatcatggt
      961 catgtacctc ggaaacgttg tggaatccgc acccgcaacc gagctattcg accatccggc
     1021 tcacccttac actcaggcgc tgatgtcggc catcccaagc tggaacccga aagatcgaaa
     1081 gctcggcctc gtcaagctgg aaggagagcc cccgagcccc atcaacccgc cttcaggctg
     1141 tccattccac cccagatgcc ggcaccggct cgccgcctgc agtaaggtca agccggaggc
     1201 ggcagaagtg agcaaggggc acttctcggc ctgccatctg catggcgtat agagagcgtt
     1261 gggcagcgaa cagcgtctgg gcaataatgt tgcccataca ctctgagaag gaacgtcgga
     1321 gaaccacaca catctgatct atgcattacc cacatcgctg gtaacgatat acagtttggc
     1381 aacagaatga gcaccttgct cggaagactc gattacccgt gccatttcta cgaggcgctc
     1441 ccgggcggcg gtccggtcgg acgtcaaggt tgcgggcgct gcggtatacc ctccaagggt
     1501 atccgggaag cttttctccc cttaggaggc gctagacgga gattctttgc acgcacgcgg
     1561 caaaaaatct ccgctttgcg ccgtggagac tgcaagtctc tgcaggccct tacgcccgga
     1621 cctttccatg gatgcgggta acgcatggcg catttgctgc gggttccctg attccctgct
     1681 atccgatgcc tgctgctcac tccgttcagc ttcgacgacg gtgtcggtga ggcgagtcac
     1741 atggttgact gcgattgact acaagattag tctatgctac acttgactac agcagtaggc
     1801 aatacggttg catgttgcac gggagcgggg gagtacgggg atgggcagag cgaggaagct
     1861 gcggatcaac cgcgggaaga gactagcaat tcaagtcggt gggcgcgctt atgcgcgtat
     1921 tccgatcaag actcacgtcg ttacggcagc agacgatatc gttgatgtag ttcaaagata
     1981 cgcagggcct ctgctgcatc cacgggacgt gctggtgata agtgagaagg tagtatcgat
     2041 aactcagcag agagcttacc ccatcagtca gatcaagcct tcatgtcttg ctcgtatcct
     2101 gtccaagttc gtgcacaagt cgccttatgg aattgggctc ggtagtccgt ggacaatgga
     2161 gctcgccatt ggggaagcga gcccgcttcg ggtcgtgact gccgctgcca tggcaggggt
     2221 tgccaaggtt ttcggcgccc ggggggtgtt ctaccgggta tgcggctccg gcgttgccgc
     2281 aatcgatgga ccgtgcgact ataccctgcc gccctacaac cgctacgcga tccttggccc
     2341 ttcgaaaccc agcgcagtgg cggagtccgc cagcagagtc gcgggagtgc cggtcgcgat
     2401 cgtcgatgca aatgacctcg gcgttacggt gctgggggct tcgtcgggat gcctcgagcg
     2461 tgtgttcctc gcagaggttc ttgccgataa tcccctcggg caatcgtgcg agcaaacccc
     2521 gattggcata gtccgcccgg cgcgttacgt cccagttcgg ggcggcgctg tcacgcttcg
     2581 gcggtcccag gagggttggc ggagatgctg aggcgtggaa cggggcatac gtccggacga
     2641 aggcgcggag gccgggtcgc aatcgaagct gcgttggtgt tcatggtagt cgtcgcgctt
     2701 gccggggcag cgtggcgcac acgtggtcgg atggggtctg atattgccct gggctctgca
     2761 gtatcggcta tagtagtgga agtcgagtca ggggacgtgc tgtacgagca gggcgccgaa
     2821 cggcgaatac ctccggcgag cctcgccaag ctgatgactg tcctcctggc ggtggaagca
     2881 gtggagggcg gactggcgag catgctggac gttgttacag tgagtaggca cgccgcgtcg
     2941 atgggaggat cccaggtgtg gttgggcgca ggtgaacggt acactctggg tcagcttctg
     3001 gagtcggtga tgatagcgtc ggcgaatgat tctgcggttg ccgtagccga gcaccttgcc
     3061 gggagtgaga ggcagttcgt cgggttgatg acgaaaaggg ccagcgagct gggaatgtgc
     3121 agcacgcgct tcgtgaatgc cacaggtctt cctgcaggag aaggcgagca cgatggatac
     3181 accacagcct ccgacatggc ccgccttgcg cgcgaggccc ttggccacca ggtgattctg
     3241 gagtggagca gcacacggag caaggcattt cggaaccacc ccctgttcat catgagtaac
     3301 acaaatccgc tgcttggcat gtatccagga tgcgacgggc tgaagacagg ccacaccaag
     3361 gcggccgggt atcacctgat agcgactgca agagcggatg gtgtccgcct tgttgccgtg
     3421 attatgcatg cagacaacga ggaagcacgt gccacgggct gcgcacgtct tctcgattat
     3481 gggtttgcgg cattgcgcgc cggagctacg gcgagatcta caaccgaaac cggggttcgc
     3541 aagccaggag tgcgtgatcc cggagtgcgt tgacagcgtg tcacaggata cgtatactga
     3601 ctacagatat agttaagcac cgacggacgt gcgccatgtc acgactcgat gggtggagca
     3661 gagggtggta ggtatggagt taccgccttt gggcgatcag gaacttgagc tgctgcggtt
     3721 cattactgag catgcgccga tcactgtacg cgaggccgtc gacagctatg gcaaggatcg
     3781 cggccttgct cgaactacga tccttaccat gatggaaagg ctcagggcaa aggggtatct
     3841 catcaggtcc gaacagggaa cggcaaacga gtactctccc cgtctttccc gttcagagct
     3901 tatgcgcggg gtggtccgcg ggtttgtgga gaagactctt gggggcacgc tttcgccttt
     3961 tgttgcgtac ctgacgcagg atgctgcagt aagcagggat gagctggaag acctcaagcg
     4021 tcttgtggag cagctagagt cgcgggaggg aaggggcagg ggtaggcaat gagcgctgtc
     4081 ttgtacgact tgctactggt agtcatgccg cagagatggg ccggctctat gtggggcgcg
     4141 ctatggcagg gaagcgcggc gctggcagtg gtgtggatca tatgcagggc tttcccgcgc
     4201 atccccgcca atactcgttg ctggctctgg cggctcgggc tcatgaaacc ctttcttcag
     4261 cttctggctg ctccgcaggt cgatctcgct tggctgccgc gcgccagaac cgtcctggcg
     4321 agtgtggtat ctcagcgttt cgctgcggat ctgcccactc gttgcgcttt gctgatcgag
     4381 gcgagccggc ccatgatggc cctttggata gcgggcgtgg cggcggttgc ggcaagcatc
     4441 ctggctgcgc tcgccagatc acgctcgata gcggaacgtt gtgcgccaat gcgtgatgac
     4501 gagcttgacg aggtcgtagc cgagctgtgc cggaggatag gaatcagcgc acgcccggac
     4561 acggtagtcg ccgattgggc gtctgttccc atgatcctgc ggcggccgag agggcacgtc
     4621 atggttgtcc cttcggatct gctgatctcg gcgcgtcatg aagacctgcg attggcactc
     4681 gcacacgagc tggcgcacat caaacggaag gacttggcct ggaattggct tcccgcggcc
     4741 gccaaagcgc tcttcttcgt gaacccgttg gtatgggtgg ctggccgcga gctcgggatc
     4801 gcgcaggaga tggcgtgcga tgagatggct gtgcgagcct ccggtgcggg gtttgcgcga
     4861 tatggaaagc tccttctcac gatggttaag ccgcgccgca gagccgagcc aacgtgggca
     4921 gtagcgacgg tttctgcagg cgcgtcggcc aaggtgatgc gcaggcgact tatcgagctc
     4981 gggcacgcac aagatccgcc ggcgctgagg ctggcaggat ccatggtggc gctgctcatc
     5041 gtatgcgctt tggtgatgcc ctttcggatc gtggaggcac cgcgcaatga gggccagtgg
     5101 cccggagtat ctatgatcag aacgcgggag ctacttgtcg gatctttgga gcggacggcc
     5161 atggcaatca tcgtcgagcg acgcgtggcg gaaagccggg caagcctccc gcaacaccca
     5221 taacggcgag cgtagcgcca gatgccggtg aacgctcgaa cgcgcgcaca gggtgaggtt
     5281 cggctaccac ttcttcaccc atggctcgta cagctcgtat ttaggcccca tgactgagcc
     5341 gtaaagcgcg ttggccgcgg cactgaaccc acccacaaat gctctcgtgg ctcccatgca
     5401 ccggagtctt ctcaggccct ccgttaggag cgctcttcct aggcccaggc ggcgatgggc
     5461 gggcacggtg gccagcggct cgacgtaggc gctccgagtc acatcgtcaa accagatggt
     5521 gcaaaatgct cctatggcgc cgtccggcgc gacggctact aggtccaggt cgcggcggta
     5581 tagcggcgcg ttctggatgt tcctgtacca ggtgggatcg tccctgttct cgacggcagt
     5641 gttgatgtcg ccctcatgaa agcctagccc cgatgcgtag cagcgctcga gaagctccag
     5701 gccgtctccg agggaacgaa tggtgtaacc tggggctatc ggcgcatcag gtatgggttg
     5761 gcccaggtcg cgccgccact ggtgttcagc tgcactacgt ttttcgtaac ccctcttgct
     5821 caggacagac tggcggaccg agtcctgctc gggcgaccac acgcacagct tgcggactac
     5881 gtcagggcca gtcgacgcaa gacgctcctc cgcgatcgat agcatttcct cctccagttc
     5941 agctgtatgg tagtcgggat gtaccataag atgcgcctcg cccgggccgc cttcgggcat
     6001 gacgattgcg gctatttccc gctcctggcc gtcgtcgcag tcggtctccc acagatacac
     6061 aatgtcttcc agacgtacct tgtcgcagtt gaggcagaga tgccagcgag catagtccag
     6121 ccgcgcgacg tgccaacttc tctcaagccc attgttagcc atgaacacta ggcgtaggaa
     6181 ctcgcgcatt cgccagtagt cgtcttccgt ttgatagggg cggaaggatg gcgttgccaa
     6241 gtacattcac tccttctcgt tcagcacggc tatgtcgttg acgaacggtt gatcgcttag
     6301 cccgctgcct gtggctcgtt ggatcatctc gttccagtgc cacttcgctc ccggcccaag
     6361 tactgagtcg atcagccacc gaccggcctc cggcatgccg gtgagcgact tggtcccgaa
     6421 acgccgggcg attgccgact gaaattgcac cgacagtagc tggccgagca tgtagttttg
     6481 gtagtagacc ggatagagag ccatgtgtat cttggcggcc caatccggct catcgcgccc
     6541 ggcgggcggc tggaccagct gatagcggct gaccatctgc ccccacaggg tgttcaagtt
     6601 ccggcgtggg ctcgcataga gggcgcgttc gaaattgacc atcactaggc accaacggat
     6661 gaatatcagc gactcaaggg ccgacacccg agcggcggca acctccagct cgcgggcgcg
     6721 tgccggctcg acgcctgcga tctcagtaag ccagcgagca tcgtaggtct ggtcgcccat
     6781 aaccatggct acggcttcgg ttgtgagcag gtgggcgtgc tcgcggagga ggtagggcag
     6841 gccggggtcg atgtagttga agtacactcc atggccgagc tcgtgcagga gtgtgctcat
     6901 ccagtcgtga ttgtcgcgca gattgcatat gaccctgata tctccctcac ggtccatgtc
     6961 ctcgcagtat gcgtgttggt acttcccggg gcgctcgtac agatcgcttc gggagaggat
     7021 gtcgtcgacc tccattccta tcccgtcgta gaaacggcgg gcaagggcgg gcaagtcttt
     7081 gccgcagaag acgggatcca gatctacact gccatccgcc ggagcgcgct ggaagaaggg
     7141 gtctgagtag tgccaggggc cgatctgctc gggctcgacg ccgaacttgg cagccaagga
     7201 ccggtccagg cgggccttct ccgatgcgaa gacggcgtcg gtctttgcag cgagcgagtc
     7261 gaccagcgtg aacagccacg tttcgtccag ctcatcattg gcaagcgaca ttgagtagta
     7321 gttgcggtat cccatgcgcc tcgcggccct gttgcgcgcc tcgactagct cgactacctt
     7381 tggagcagct tctcgtccga tctgcttgga ggcttcccat gccttgagcc gcctaaggtt
     7441 gtcgcgctct tcggcaagca cctggttgat gtcgttctcg gacgccggct ggccctcgag
     7501 gtcggccctg aagtgcacga agatgtcggt gatctcggcc tgaagatcag tctgttcagc
     7561 gatctcctcg ggagtcatct ggtttcgggc gtacatccgg gcaagcacag aaacctctcg
     7621 ggcgagcagc gggtcgaccc cgtcaggtgt tcccaccgtc tcagattgag cctgttgagc
     7681 ctctcgcagt attcggtaat cgccgggatc cgaaagcagc tgggcaaggc gcttcctggc
     7741 ttctgtggca acacggcgct gcccagagtc gccggtggtg tcggcgttcc aggtggccaa
     7801 gttgatcacc ttggcgaggt tgctgatgga ttccacacga tcgtcaacga atgcgcggat
     7861 gtctgttgtc aagtgaagca cctccaaata ttccacgctg caattacaaa caccttgctg
     7921 acctcatgtg cacaaggctg ccgagcccgg tctaccctgt gaaggcgcta tggtcattga
     7981 cacaaccgca acaggatcca tcctgaacgg tcaagcacca acccgcggcg gatagtcacg
     8041 acctcgatca gagccttagg gcccaaggat tccaggtcgt gctgcgggat aaggaacacc
     8101 gcttcccagg gtcggcgctg tccacttgac ggcgtagtct cgtaggtcag ctccttggct
     8161 gtcgggcgta gccgctgtcc gtacagcagc ccggacaatt ctggggctgg gtcgcatgat
     8221 acgatctgaa ggcggtacgt tacggaggga ctgggtcgcc acgccaggac tgcagtgacg
     8281 gcgacaccgt catcggttcg attagccaca acggacctga tctcgccgct cggagggaga
     8341 acccggaatg gactcggatc aggccgagtg ctgggggcat cgtttttgtg cgtccgtccg
     8401 aacacctgcg gcggcgccag gtcgagcact ggtactgaac agaatgactc attggttctg
     8461 atgaaactct tcagaaaagc ggagagaccc gatagttggc tcctgtactt ctcgagcgct
     8521 ttgagcttcg cggcaattgc gtcctcggtc agctcagcac ttatccatgc tgttggaggt
     8581 gaatcccgcc ctctgggcgg atcaagtggc agatcgggcc ggtaccccca cggctggggc
     8641 cagtcaggac cggtgtgtaa catgtaggcg agtacgcttg cgccggtgag ctccacgtag
     8701 ctgagctgct cgattgtgta catggtgaga gcataggtag cccagtggtc gctgtgtgtg
     8761 tcattggggt gggggacgag caccatgtcc ggcatgtact cggccagtat cgttgcgagg
     8821 tcgtctacta ttcttgaggc gcagtactgt gcgtctggcg tgaatgaaga tacataggga
     8881 ttaccggcat gccctgtgtg cttcgatgtg taaggcttgg ccggccccca gtgccggttg
     8941 tctatgatgt cggcaagtcc gccgtcaggg tagccgagga acacaatgtc atcttcatcc
     9001 acgccaagaa ctcccagtgc agcgacggct tcgttgcggc ggcgtagccc aagagccacc
     9061 atatcatgga gcgagggccg cgccgtgcca aacaacatgc ctgccgcaac ggcggatccg
     9121 tcgcccatgg tcatgaacac cgtctttacg ttcgctcctc tccgacaggc ggagtagatc
     9181 agtcctcctg cccccaaagt ctcgtcatct ggatgagggg ctatcacaag gatacgtgat
     9241 gccgatgcga gtggggcagc tgcatgggcg gacccgacga gcagcgcaag cccgacgcat
     9301 acggcggcaa gcctcagcca ggcaactgtt gcccgggagt atttcggctt ccgcgcatgc
     9361 caagggatgt tgcatgttct catgctgaac cacttaggcg cctgcgtact tcggcgcata
     9421 tgcgacgatg ggaaacagcg cccgggatag cttcggtaat ggggaaatgg attcctgcca
     9481 tgttatcgcc gaaactgtgg caatcgctac cccatatgcg gatcgcggtc cgatccgcac
     9541 tgtgagccta ttcctgtcct cagcaactca acgtagacca tgaattcctt aatgatctgt
     9601 tcccagtcga gtgatgagag gtcgcctctg ccgaggtact cctgttcaag gcccttcagg
     9661 aagagcagga cgaacctcag gaggcactcc gcatccacgt cggggcgcag ctgagcgaga
     9721 tcaaggccag ttaggaatgc cttcatgccg aagtcggtca actcagcctg caccgaccgt
     9781 atctcctgct taagctcatg ggggggatta gagacggcat ccgcgaggaa gtgccatcct
     9841 ttggggtctt cccggtacag ctccaatttg atcagagtaa gtcgcagcat gcgctctatc
     9901 acgtcaccgg gcagttcgcc cgcgcgtgac agcagcgtct cggtggttct ttgcactact
     9961 tccctgaaca cagtcatgta gaggttcttc ttgctgccga agtagtggaa gagcaacccc
    10021 ttggagatcc cggccttcct cacaatctcg ttggtagacg caacctcata gcccttggcg
    10081 aactcctcga tgctggctcg aatcactc
//
LOCUS       DAOTAC010000059        10042 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_5711_length_10042_cov_4.804546, whole genome shotgun sequence.
ACCESSION   DAOTAC010000059 DAOTAC010000000
VERSION     DAOTAC010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 10042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10042
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5711_length_10042_cov_4.804546"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(209..3052)
                     /gene="polA"
                     /locus_tag="PLM74_03905"
     CDS             complement(209..3052)
                     /gene="polA"
                     /locus_tag="PLM74_03905"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015254251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HON42261.1"
                     /translation="MTERKARKILIIDGHSLAHRAFHALPPTLSRADGTPTNAVLGFC
                     NMMVKLLEQEQPDVGICAFDRPSPTFRHEEYEQYKATRKPMDESLRVQMPIVREAARA
                     FGFSVVELDGWEADDVIGTLSRMADEAGDEAIIVTGDRDALQLASDRVRVVLTKRGIS
                     EFEEYGPEEVRARYGFGPELIPDFKGLMGDVSDNIPGVPGVGEKTAMRLVSEIGTVED
                     VLAGAHSIKQERLKNALLENAEQARASKHLATIDRKAPVGVDLEQCCEVRRDPGHLAE
                     FLRKQDFRLLLSRLGLVGAGPGDRAGQASLFGAAPSGEAAQGVGGGSEVEASNGEGGA
                     PGSENVQIVTTQEGVEELAARLRRAGRFAFDVVSVGARTMTARVVGLAFVTPGVSSYV
                     PIGSSEDRADGDRVGASDAGRVNPHMAANALRPVFADAAIEKSCHDVKEKIILLRSIG
                     CELRGVSFDSMIAAYLVDPAAPNDEMCDLAEKWMAVSVPRVRESIRQEAKRTRDPESC
                     VGAEVAAGMCARCLAVMPQLEATLLASVSSYGMDRLYRDVEMPLATVLADMEMTGVKV
                     DAARLRELSSEMDSILNQLVAEIYRHAGEEFNINSTKQLGYVLFERLGLPPVKKTKTG
                     YSTDAEVLETLAELHPVVGKVLEYREISKLKSTYVDALPELINPRTGRIHTTFNQTVA
                     ATGRLSSTDPNLQNIPVRTEEGRKIRDVFIPGEDGWVIISADYSQIELRVLAHISQDA
                     TLIQSFLRDEDIHRRTAAEVFGVDFEDVTPEMRSRAKAVNFGIVYGISDYGLARNIGV
                     KPAEAKEFIERYFDRYSGVRRYMDSIVAQAKLDGYVTTLMNRRRLLPELSSPSFPVRK
                     FAERVAMNTPIQGSAADIIKRAMVNVHARLTKEGFRARLLLQVHDELVLEAPADEVQA
                     VATLVAGEMSESVQVAVPLKVDVATGKSWMDAK"
     gene            3233..4444
                     /locus_tag="PLM74_03910"
     CDS             3233..4444
                     /locus_tag="PLM74_03910"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HON42262.1"
                     /translation="MALTAASRKLIMALALAAALILGIAVGPSASARAAVSDISGHWA
                     ESAISTLVKMGVVQGYPDGTFRPEGLVNRAEFAKIVARAFGYAPTVDTRFRDMDGHWA
                     EPFVSALDASEVVTGYPDGTFRPAQNVSRAEMAAILARVAQLGKVGEADATAWNPSFR
                     DVDSTHWAYRYVEVARRLDIIPLHFGLVFEPDRPATRAETAHMVNALAQAQFARGTVS
                     GVSKATETINIKNAAGTDQVVQVDPDTVIYRNGVVSDLESVRSKDSVYAVGTGYGAAR
                     FVLAEGQVTREDITKKVSAITRGVITPEQVDAISRGKWDEARKGMSPALAARLVEMGL
                     TDDEAQALLSQDWESLPELGQTRLAQALSEEVGVSSELIEAVMRRDWKAARSYAELEA
                     AQILLSRFLDM"
     gene            4609..6045
                     /locus_tag="PLM74_03915"
     CDS             4609..6045
                     /locus_tag="PLM74_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42263.1"
                     /translation="MIRSRASLIAVLACAAVTIGLALAAHGPAPAQAAKGDGRSVELT
                     IYNSDLALVKEKRAMEIPEGLSTIMFRDISARINPATVSFASLTAPQAVRVREQNFEY
                     DLVEDVKLMRKFVGERITVRTRSGEVFTGYLLTGARGSSASNIVMSSGPDGTGQVFTV
                     YLGDVQSVEYPSLPEGLITRPTLAWLMSNTSGATTHNVLVTYLTGGLSWAADYVAVIG
                     PNDDFIDLRGWVTITNESGAAFKDAKLKLVAGDVHQVREEMYDVVRASGAKLAMAEAG
                     PSFEEKAFYDYHLYTLQYPTTLKENQVKQIELLAADKVPAKKLLVYDGARFGTKVNSI
                     LEFWNKQDGNLGMPLPKGVVRVSKMDSDGSLEFIGEDRIDHTPKDEKVRITLGSAFDV
                     IGSRTQVSTKRISDRVREETWKIEVRNHKEEPVEVTVLERMWGATQWEITTASAKWNK
                     LDSQTAEFPTKIAAGGTATITYTVRYSY"
     gene            6385..9162
                     /locus_tag="PLM74_03920"
     CDS             6385..9162
                     /locus_tag="PLM74_03920"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HON42264.1"
                     /translation="MIPIPDSVLDRKMSLTPEAITILESRYLLKDKEGNIVETPEQMC
                     LRLAREAARAGVRYEGTRETEDERVRLFYWLLVDELFSPNSPTWMNAGTANSQNAACF
                     VLPVEDSLPQIFDAVRGAALIHQSGGGTGFDFSSLRPKGDVVRSTGGVASGPVSFMSV
                     FNAATETIKQGGKRRGANIGILRVDHPDIESFITCKEDNAALSNFNISVGITDSFMQA
                     VEDDAEYELINPRSKKATGTKRASEVFTKIVNHAWRNGEPGVVFLDRLEEGNPTPALG
                     RITATNPCGEQPLLPYEACHLGSVNLQRMYREEPLSDDDVLSELSLSDSFAEVCHNVL
                     AAEMRHINWQLLRTAVHGALDFLDSIIDVSEYPLPEISQMVRRTRKIGLGFMGLADLF
                     ILLGVAFASEKAELLAELVSRFITHEARLKSQQMAVDRGPFPAFAESRYAEMGEPPMR
                     NATVTTVAPTGTISMVANASSGCEPLFGVAFTKQNVLGGKSLTQVNSLFLEDAKKRGI
                     YSEDLMERIAENGGSLSGISGIPDDMVALYRTALEIPWEWHVRIQAAVQRGIDNAVSK
                     TINLPGNATPDDVRAALTLAHRLRCKGITVYRSGSREVEVIATKAKATAGAEVDLSYG
                     AAGATGVAGSGLDDLCGSPTPRGKKPYTVDESLWGRLQPIDRPETLCGITKRIATPSG
                     TLWATLNLYDQRPFEMFCQIGRAGTDIYAFTESIARLISLALRAGVDPSEIARQLRGI
                     GGSGQVGFGPNKVLSVPDAIGRMLDQTIQARYAGAEWATPSQRGIAGQSGNDEDPNAA
                     LSSTSTFTSTSTLGDALLALSGDVSAVQATLLFADESNGRGQDYGNAGTSGGAISDEA
                     RSESAVLETATAAEAQSAYSGSSSGQNTQADSHSGKKATRYDICPQCGNYSLIYASGC
                     SECRLCGYSAC"
     gene            complement(9666..>10042)
                     /locus_tag="PLM74_03925"
     CDS             complement(9666..>10042)
                     /locus_tag="PLM74_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42265.1"
                     /translation="QKKEAPLPARIQTIIACQTGQDLNRMLEKPVQIWAGCPRTPLQP
                     TTVERHAQKYPWIRVMHRGYPTGFLAEPVGHRTVLHAQAFPKATDGDASFSRPGCEGG
                     FAGGRSTAAEGNTHLSRPHAGP"
BASE COUNT         2124 a         3135 c         2817 g         1966 t
ORIGIN      
        1 atcctaagag caaaccaggg gtgcacctcc tttcgggctt tcaggatcga aaggcatatc
       61 tagactcaaa tgtgatactc ccgatgtgct gggaggtaag accgaaacga gccatttagc
      121 tcatacggct gttctattct acactcaacg cgtaattcct tcaaggtgcg gcatcgtcat
      181 ccaacaattt tcgtgcctgc atgaagcgct atttcgcgtc catccacgac tttccggttg
      241 ccacgtcgac tttcaaaggc actgccacct gcactgattc gctcatctct ccagcgacta
      301 gagtcgccac tgcctggacc tcgtcggcgg gcgcctcaag taccaattcg tcatgtacct
      361 gcagcaggag tcgcgcccga aaaccctcct tcgttagccg tgcatgcaca ttcaccatcg
      421 cccgcttgat gatgtcggct gccgaaccct gaatcggcgt attcatggcc acgcgctccg
      481 cgaacttccg cacgggaaat gacgggctgg atagctcggg cagaagccgc ctgcgattca
      541 tgagagtcgt cacgtaaccg tccagtttgg cctgggccac aatggagtcc atataccttc
      601 gaacaccgct gtacctgtcg aaatacctct cgatgaattc cttggcttca gccggtttca
      661 ccccgatgtt tctcgccagg ccgtagtcgc tgatgccata gacgattccg aagttgaccg
      721 ccttcgcccg cgacctcatt tccggagtaa catcctcgaa atctaccccg aatacctcag
      781 ccgctgtgcg ccgatggata tcttcatccc ttaagaaaga ttgtatgagt gttgcatcct
      841 gagatatgtg tgcaagtact cgaagttcta tctgagaata atcggcgctg ataatcaccc
      901 acccgtcctc gccagggatg aacacgtccc tgatcttccg tccttcctcg gtgcgcacgg
      961 ggatgttctg aagattagga tccgtactgg agagcctgcc cgtggcggca acagtctggt
     1021 taaaggtcgt gtggatccgc ccagttcgtg ggttgatcag ctccggaagc gcgtccacgt
     1081 atgtagactt caacttggag atctcacgat actccagcac cttgccgacc accgggtgca
     1141 gctccgcaag ggtctcaagt acctcagcgt cggtggaata gccggtctta gtcttcttga
     1201 caggaggcag acccagtcgc tcgaagagca cgtagccaag ctgctttgtg gagttgatgt
     1261 tgaactcctc gcccgcgtgc cggtagatct cggccacgag ctgattcaga atggaatcca
     1321 tctcactcga cagctcgcgt aggcgagccg cgtccacctt gacccctgtc atctccatgt
     1381 cggcaagcac agtagccaat ggcatctcca catcgcggta taggcggtcc attccatatg
     1441 aagagactga ggccagaagt gtcgcctcaa gctgcggcat gaccgccagg cacctggcgc
     1501 acatcccggc agcgacctct gccccgacac agctttccgg gtcacgcgtt cgcttagctt
     1561 cctgacgtat ggactcgcgg actctgggaa ccgacaccgc catccacttc tcggccagat
     1621 cgcacatttc gtcattgggc gcagccgggt ccacgaggta tgcagcgatc atgctgtcga
     1681 aggagacgcc gcgaagctca cagccgatcg aacgaagcag gatgatcttc tccttcacat
     1741 catggcaact cttctcaatg gcagcgtcgg caaacaccgg tcgcagcgca tttgctgcca
     1801 tgtgcgggtt cacgcgaccc gcatcactcg ctcccaccct atcgccgtcg gctctatcct
     1861 cgctactgcc tatgggaacg tatgacgaga ctcccggggt cacgaaagcc agcccgacta
     1921 cacgggcggt cattgtccgc gcgcccacgc tcaccacgtc gaaggcgaac ctgcctgccc
     1981 tccgcagacg cgctgccagc tcctccacgc cttcctgagt ggttactatc tgtacatttt
     2041 cgcttccggg cgcaccgcct tcgccgttgg acgcctcgac ctcactgccc ccgccgactc
     2101 cctgggccgc ttcgccggaa ggcgctgctc cgaacaagct ggcctgcccg gcacgatcac
     2161 ccgggcccgc ccctacaagc cccaatcgcg agagcagtag tcggaagtcc tgcttgcgga
     2221 ggaactcggc caggtggccc ggatcgcgcc gcacctcgca acactgctcc agatcgacgc
     2281 cgaccggagc tttcctgtcg atagtggcca gatgcttgct ggctctagcc tgttcggcat
     2341 tttccagcag cgcgttcttc agcctctcct gctttatgga atgggcgccg gccaacacgt
     2401 cttcgactgt gccgatctcc gaaaccagtc gcatcgcggt cttctcgccc actccgggca
     2461 cgccaggaat gttgtcggac acatccccca tcagtccttt gaaatccggt atcagctccg
     2521 gcccgaaccc gtacctcgcc cgcacttctt cgggcccgta ttcctcaaat tccgatattc
     2581 cgcgcttcgt cagcaccacc cgcacacgat cggaagccaa ctgcagcgca tcgcggtcgc
     2641 cggtcacgat gatcgcctcg tccccggcct cgtccgccat ccgcgacaat gtgccgatga
     2701 catcatcagc ctcccaaccg tccaactcga ccacggaaaa gccgaaagcc ctggctgctt
     2761 cgcgcactat cggcatctgg acccgtagcg attcgtccat cggcttcctg gtagccttgt
     2821 attgctcgta ctcttcatgc ctaaaggtgg gcgacggccg gtcaaaagcg cagatcccca
     2881 catccggctg ctcctgctcc agaagcttca ccatcatgtt gcagaacccc agcaccgcat
     2941 tggtgggcgt gccgtcggct ctggaaagcg tggggggcag ggcgtgaaaa gccctgtggg
     3001 ccaggctgtg gccgtctatg atgaggatct tgcgcgcctt gcgttctgtc aaatccgctc
     3061 tccctttccg atccagtcta ctccagtcgc cctcatatcc atttagtttt ccacacgagc
     3121 gccggcaatc cctcctccct cctgtcgtcc catcacttgc ttgcaggaaa cgtcatatgt
     3181 atggcgaaaa gcaccatacg catgccaagc tgcgatgaaa ggagttatga caatggcttt
     3241 gactgcagct tcgaggaagc tgatcatggc gctcgcgctc gccgcggccc tgatactggg
     3301 catcgcagtg ggcccatctg cgtctgcccg agcggccgtg tccgacatct ccgggcactg
     3361 ggccgaatcg gctatatcta cattggtcaa gatgggcgta gtgcagggat atcccgacgg
     3421 gacattccgc cccgagggcc ttgtaaaccg ggcagagttc gccaagatcg tggcgcgcgc
     3481 cttcgggtac gcacccacgg tagatacacg tttccgcgat atggacggcc actgggctga
     3541 gccattcgtg tcggcactgg atgcatccga ggtagtcacc ggctatccgg acggcacatt
     3601 tcgaccggca cagaatgtat cgagagccga gatggcggct atcctcgctc gcgtcgccca
     3661 gctgggcaag gtgggcgaag ccgacgccac ggcctggaac cccagcttcc gcgacgtaga
     3721 ctcaactcac tgggcgtata gatacgtaga ggtagcccgc cgtctggaca tcatcccgct
     3781 ccatttcgga ctggtgttcg aacccgatcg cccggccacc agggccgaga ctgcgcacat
     3841 ggtcaacgcg ctagctcagg cgcaattcgc gcgagggact gtcagcggcg tcagcaaggc
     3901 tactgagacc atcaacatca agaacgcggc cggaaccgac caggtggtac aggtcgatcc
     3961 ggacaccgtt atataccgaa acggcgttgt ttcggatctg gagtcggtgc gttccaagga
     4021 ctccgtctac gccgtgggaa cgggctacgg cgcggcacgt ttcgtgctgg ctgaaggtca
     4081 ggtcacccga gaggacatca ccaagaaggt gagtgccatc acccggggcg tcatcacgcc
     4141 cgagcaggtg gacgccatat cccgcggcaa gtgggacgaa gcgcgcaaag gcatgagccc
     4201 ggccctggct gcgcgtctag tggaaatggg gctgacagac gatgaggcgc aggctcttct
     4261 cagccaggac tgggagtccc tccccgaact ggggcagacc cgcctcgccc aggccctttc
     4321 ggaggaggtc ggtgtatcgt ccgagctgat cgaagccgtg atgcgccgtg actggaaggc
     4381 ggcccgaagc tacgctgagc tggaagcagc ccaaattcta ctatcacggt tcctggatat
     4441 gtagcggggc cagcataatc tgtcgaatac acgtccgcag agccgccgcg ccggaacaaa
     4501 cggtgcggcg gcatcgtctc tacaagtgca aaacggagtc tcaacggagt cgcatatgat
     4561 ctcatggaat ctcattacag aatctcacta ccgggagggc gatacgatat gatccgaagc
     4621 cgagcaagtc tcattgcagt actggcctgc gccgccgtca ctatcggcct cgccttggcc
     4681 gcgcacgggc ccgcgccggc ccaggcagca aagggggatg gaagatcggt ggagctgact
     4741 atctacaact ccgatctggc ccttgtgaaa gagaaacgag ccatggaaat ccccgaaggg
     4801 ctgtcgacca tcatgttccg cgacatctcc gctcggatca atccggctac cgtcagtttt
     4861 gcatcgctga ccgcgccaca agcagtgcga gtgcgagagc agaacttcga gtacgatcta
     4921 gtggaagacg ttaagctcat gcgcaagttc gtaggcgagc gtatcaccgt gcgcacgaga
     4981 agcggcgagg tcttcacagg ctacctactc accggagccc gtgggtcgtc cgcctccaac
     5041 atcgtgatgt cttccgggcc tgacggcacg gggcaggtat tcacagtcta cctcggcgat
     5101 gtccagtctg tagagtatcc cagcctcccc gagggcctca taacccgacc cacactggca
     5161 tggctgatgt ccaacacgtc gggggcgacc acccacaacg tgctggtcac ctacctcaca
     5221 ggcgggctat cctgggctgc cgactacgtg gcagtcatcg gcccgaatga cgatttcatc
     5281 gatctgcgcg gatgggtcac catcaccaac gaatcgggag cagcgttcaa agacgccaag
     5341 ctaaagctgg tagcaggcga tgttcatcag gtacgggaag agatgtacga cgtggtcaga
     5401 gcctcgggag cgaaactcgc catggccgag gccggcccat cctttgagga gaaggctttc
     5461 tacgactacc atctgtacac gttgcagtac cctacgaccc tcaaggaaaa ccaggtgaag
     5521 cagatcgagc tcctagctgc cgacaaggtt ccggcgaaga agttgctggt gtatgacggc
     5581 gcgcgattcg gaacgaaggt caactccata ctggagttct ggaacaagca agacggtaac
     5641 ctgggcatgc cgctgcccaa gggagttgtt cgcgttagca agatggacag cgacggttcc
     5701 ctggagttca tcggcgagga caggatcgac cacactccca aagacgaaaa ggtccgcatc
     5761 acactggggt cggccttcga tgtcataggc tcgcgaaccc aagtgtccac gaagcgcatt
     5821 tccgacagag tgcgggaaga gacctggaag atcgaagtgc gtaaccacaa ggaggaaccc
     5881 gtagaggtta ccgtgcttga gcggatgtgg ggcgccacac agtgggagat cacgaccgca
     5941 tctgcgaaat ggaacaagct agactcccag accgcagagt tcccaacaaa gatcgccgca
     6001 ggcggcacag ccacgataac gtacaccgta agatactcat attagccttt tggcctcgat
     6061 cgcggagggc aaacgctaac gcatcagcac cgaatccaat cgcaccagag caagccgtac
     6121 cagtactacc ccccaaggta aggccgccga ttgactcggc ggctttaccc tttctatgat
     6181 tcaccttcta tgattcaccg tgctgcagaa aaccgcagct gatggctttg tgacgaacac
     6241 catacccaca tctaccagct gtttttatga tactgccaag ggcttgcact gctgccgaca
     6301 accacgcacg acacacattt tcgtggtatg atagtgatac ttggtggatc gaacacgttt
     6361 tcgcacaaga caggaggcag tgcaatgatc cctattccgg actctgttct agaccgcaag
     6421 atgtcgctca ctcctgaagc gatcaccatt ttggaaagca gatacctgct gaaggacaag
     6481 gaaggcaaca tcgttgagac ccccgagcag atgtgcctcc ggttagcccg agaggcagcg
     6541 cgagccggag tccggtatga gggaacccgc gagaccgaag acgagcgtgt ccgcctgttc
     6601 tactggctgt tggtggacga actgttttcg cctaactctc ccacctggat gaacgcgggt
     6661 accgcgaact cacagaatgc agcctgtttc gtgctgccgg tggaagactc cctgccgcag
     6721 atcttcgacg cagtgcgagg cgcagctctg atccatcagt ccggcggcgg cacgggattc
     6781 gatttctcga gcctcaggcc caaaggcgac gtcgtgcgct ccacgggcgg agtcgcatcg
     6841 ggcccggtat cattcatgtc ggtgttcaac gcagctactg aaactatcaa gcaaggcggc
     6901 aaacggcgcg gcgccaacat aggcatactc cgcgtcgacc atccggatat cgagagcttc
     6961 atcacatgca aggaagacaa cgcagcgctc tccaacttca acatctcagt gggtatcacc
     7021 gatagtttca tgcaggcggt cgaggatgac gccgaatatg agctgataaa cccgcgttct
     7081 aagaaggcga cgggtacgaa acgcgcgagc gaggtcttca ccaagatagt caaccacgca
     7141 tggcgcaatg gcgagccggg cgtggtcttc cttgataggc tggaagaggg caaccccacc
     7201 cccgccctgg ggcgcatcac agccaccaac ccctgcggcg agcagccgct gcttccatat
     7261 gaagcctgtc atctcggcag cgtcaacctg cagcgcatgt acagagaaga accattatca
     7321 gacgacgacg tgctgagcga gctatccttg agcgacagtt ttgcagaggt gtgtcacaat
     7381 gtgctggcag ccgagatgcg ccacatcaac tggcaactgc ttcgcacagc cgtgcacgga
     7441 gccctagact tcctggattc aataatagac gtgagcgagt atcctctgcc cgaaatcagc
     7501 cagatggtga ggaggacacg caagatcggg cttggcttca tgggcctggc cgacctgttc
     7561 atcctgctgg gagtggcgtt cgcctcagaa aaggcagagc tccttgcgga gctggtgtcg
     7621 aggttcatca ctcacgaggc ccggctgaag tcgcagcaga tggctgtgga tcgcggtccc
     7681 ttcccggcct ttgccgagag ccggtacgcc gagatgggcg aacctccgat gcgaaacgcc
     7741 acagtgacca ctgtcgcgcc taccggcacc ataagcatgg tagccaacgc atcatcaggg
     7801 tgcgaacctc tcttcggagt ggccttcacc aaacagaacg tgttgggcgg caagtccctg
     7861 acccaggtga actcgctttt cctggaggac gccaaaaagc gcgggatcta ctctgaagac
     7921 ctcatggaga ggatcgctga aaatggcggt tcgctttcgg gcatctccgg gatccccgac
     7981 gacatggtcg cgctctatcg cacagcgctg gagataccct gggaatggca cgttcgcatc
     8041 caggcggcag tgcagcgcgg catcgacaat gctgtgtcca aaacgatcaa cctaccgggc
     8101 aatgcaaccc ccgacgacgt gcgggcagca ctgactctcg cgcataggct ccgctgcaag
     8161 ggcattacgg tgtacaggtc aggtagtcga gaagtagagg tcatagccac aaaggctaag
     8221 gcaaccgcag gggccgaagt tgacctgtca tacggtgcgg ccggcgcgac cggcgtcgcc
     8281 gggtcggggc tggacgatct atgcgggtct cccactcccc gcggcaagaa gccgtatacc
     8341 gtcgacgagt ccctctgggg aaggctgcag ccaatcgacc ggccggaaac cctgtgcggg
     8401 atcaccaagc gcatagccac tccgtccggc acactctggg ctacgctcaa tctgtacgac
     8461 caacgtcctt tcgagatgtt ctgccagata ggcagagccg gcaccgacat atatgcgttc
     8521 accgagagca tcgcccgcct catctctctg gcgctgcgtg caggcgtcga tccatccgaa
     8581 atcgcgcgtc agttgcgcgg aatcggcgga tccggccagg tgggcttcgg cccgaacaag
     8641 gtgctctccg tacccgacgc aatcggacgc atgctggatc agaccatcca ggcgcgctac
     8701 gccggcgccg aatgggccac accttcgcag cgcggcatag ccgggcaatc cggaaatgac
     8761 gaagatccca atgcggcgct gtcatccaca tccacattca cgtccacatc gacgctgggc
     8821 gatgcgcttc tcgccctatc cggggacgtc tccgcggttc aggccacact gcttttcgct
     8881 gacgaaagca acgggcgcgg acaagactac gggaacgcgg gtacctccgg cggagcaatt
     8941 tcggacgaag ccagatcgga gtcggcagtc ctggaaaccg cgaccgcggc cgaggctcag
     9001 tctgcgtatt ccggcagcag cagcgggcag aacacacagg ctgacagcca ttccggcaag
     9061 aaagctaccc gctacgacat ctgcccccag tgcggcaact actccctgat atacgcctcc
     9121 ggttgcagcg aatgcaggct ctgcggctac agcgcgtgct aaagcgcgca ggaagaacgc
     9181 tcatcaagtc catgttcggg tccgggccta tgcttggccc agaaccgggc ctgcgttcgg
     9241 tcgaggccca ggtcccggcc tgcgtctagg tctaagtccg agtccacggc tgctcggctg
     9301 tagctggctg gaataatatg gtctgttcac attctgaacg cattcttgcc gatctgttca
     9361 ttttcgctgc aggtgctgat gcataatgaa atgcatagct gcatagagtg ccggaagcgt
     9421 cgcggctatg gtgtttcgtc ctatttccgc tgtcctaatc cggcatttcc ctatatttat
     9481 ccccaagtcc catatatcat tttacaccat tcgcggttac ccaggtcgaa tcgcttactc
     9541 gtcccgcttt atgcacctat gcgtcgcatt atgcatcagc tatcgtccgc tttcgcgacg
     9601 cattcacgaa aaagcgttca taaaacgaac agatcacaaa acgccagcta cacgagccat
     9661 ggcgcctaag ggcccgcatg cggacgagag agatgtgtgt tgccctcggc ggcagtgctc
     9721 ctccctccgg cgaacccgcc ttcacacccc ggcctggaga acgaggcgtc gccgtccgtg
     9781 gccttcggga aagcctgcgc gtgcagaacc gtacggtgtc cgacaggctc ggccaaaaaa
     9841 cctgtcggat agcccctgtg cattacccgt atccatgggt atttctgggc gtgacgttcc
     9901 actgtggtgg gctgtagcgg ggttctgggg catcctgccc atatctgaac aggcttttcg
     9961 agcatcctgt tcagatcctg cccggtctgg caggcgataa ttgtctgaat tcgcgccggc
    10021 aacggcgcct cctttttctg gc
//
LOCUS       DAOTAC010000060        10041 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_5712_length_10041_cov_4.646405, whole genome shotgun sequence.
ACCESSION   DAOTAC010000060 DAOTAC010000000
VERSION     DAOTAC010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 10041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10041
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5712_length_10041_cov_4.646405"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="PLM74_03930"
     CDS             <1..582
                     /locus_tag="PLM74_03930"
                     /inference="COORDINATES: protein motif:HMM:NF014042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HON42266.1"
                     /translation="GALLRDGREVLGETSVGSSTSRIERVFLRPANPRPLPEALRAIA
                     EADAIVLGPGSLYTSVMPNLLVDQVAEAISASEAVKIYVSNVMTEPGETQGYTAQDHL
                     AAIFRHAGPDLVDWIIVNDAPITDGMMRKYSYEGAEPVMWDREVLESMGIQVVEAPVA
                     SQTELVRHDPDAIAAEVMALVASTRGASTATCE"
     gene            complement(569..1099)
                     /locus_tag="PLM74_03935"
     CDS             complement(569..1099)
                     /locus_tag="PLM74_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HON42267.1"
                     /translation="MRQLTSLSYYVVLLLLVGIPVYGAMKGIDVYSCFIAGASDGLRT
                     IARIAPAMIAMFVAVGMFRDSGALDAAARALSPAARALGIHPDLLILAALRPISGSGA
                     LGYLAKLLAAYGPDSAEGLLASVIQGSTDTSLYIIALYFGSVGITRTRHTVPAALLAS
                     LAGFLAAVFICRFTHM"
     gene            complement(1096..1683)
                     /locus_tag="PLM74_03940"
     CDS             complement(1096..1683)
                     /locus_tag="PLM74_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HON42268.1"
                     /translation="MMGIIWVAMLAIGTIWSAVARGPQAVTAMAISSTKEAVQLCLAL
                     AGSIGLWSGMAQIAQQSGLTTALASAIRPLIGPLFPELEPKSKSTPLIAASMAANLLG
                     LGMAATPLGLSAMQQLAIESRTQSEPSDAMCTFVIITASSLTLVPTTIISLRAEMGSA
                     DPAGVIGATIVATACSTAVAVIVDFALGKRGRKNR"
     gene            1849..2829
                     /gene="whiA"
                     /locus_tag="PLM74_03945"
     CDS             1849..2829
                     /gene="whiA"
                     /locus_tag="PLM74_03945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HON42269.1"
                     /translation="MDFYSLMKDELVRAMPERGCCRVAELSALLMADGVIRTSEGHVS
                     IVISQTHAGVARKVVSLIRDLEWKALERPGVEVAVRYRKRKGAPRGNLYVVRIANHDE
                     AIDLLDRLGLGLGLAGPDGLVHYAERQVLARRCCKRAYIRGVFLGAGYMTDPSKGYHL
                     EIAVRGLENAEYISGLLAEFGIRAGIVPRRGRSVVYLKDGDDIVELLRVMQASSALLE
                     LENARILRSIRGDVNRLVNAENANLAKTVDASLGQIRDIQLIDDVMGIRRLPPTLAAA
                     ARLRLENPEASLTELGAMLSPPVSKSGVNHRMRRIARIADDIRLQSRRER"
     gene            2842..4239
                     /gene="rpoN"
                     /locus_tag="PLM74_03950"
     CDS             2842..4239
                     /gene="rpoN"
                     /locus_tag="PLM74_03950"
                     /inference="COORDINATES: protein motif:HMM:NF009118.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HON42270.1"
                     /translation="MSYGMVQQQVQKLVMTPQLRQAIQLLQLPTVDLAQYVQNELLGN
                     PVLEEADQPAEGSADENQASRADDDQIDWAGYFAGDGGADSGWPSDLSDRDSDDDPVR
                     LIRWEPSLAERLHVELRVASTCPRTLCIGEYLLGCIDDSGFFRGDVADVAAALGVPEP
                     EVEEVLHLIQCLDPVGVGSRSVRECLELQISAKSAPDDMRALAMRIVREYLDDLAEGR
                     ILRIASELGADVAQVQEAADMIRALSPRPGQDYAGGGQARYIVPDVIVEKVGDDYVVT
                     SCDSASPRLVINSAYRAMLSDARTDTAARDFIRERLNSAAWLIRALGQRRDTITKVAT
                     SIVAHQRDFFDRGILHLRPLTLREVAVDIDMHESTVSRATAGKYAQTPRGTFELRFFF
                     GSGVSTLGGDTASAASIKKYIKEMIASEDAKSPLSDQEIADALTGEGICISRRTVAKY
                     RGELAIPASTRRRRY"
     gene            4385..5422
                     /locus_tag="PLM74_03955"
     CDS             4385..5422
                     /locus_tag="PLM74_03955"
                     /inference="COORDINATES: protein motif:HMM:NF016114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HON42271.1"
                     /translation="MGKTADIIAVQCRIAPELTERIVLRHNILRHVSYSQPIGRRALA
                     QCIGRSERAVRREENALREVGLILVGSDGIRLSRDGEDILWELGEYVRLLTGTGELER
                     TLAGRFGLEQAIVVTGDSDRDDAVKREIARAGASVLRDVISDGDVVAVTGGTTMAALA
                     QEAVEVRSVRDLVVVPARGGLGEDVEKQADTVAASLAKKLGGSYRLLNLPDDLGLESA
                     ASVREEPRIKQVLDTIRQARVVVHGVGTALEMAKRRNLSEAEIDHIKQLGAVGESFGF
                     YFNSAGEVVYTTSSLGLRLADLSSVPTVICVGGGASKAQALSAVLRNGFSHIVVTDEG
                     AAREINREQGR"
     gene            5450..6454
                     /gene="gap"
                     /locus_tag="PLM74_03960"
     CDS             5450..6454
                     /gene="gap"
                     /locus_tag="PLM74_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HON42272.1"
                     /translation="MAVKIGINGFGRIGRLVFRAGEKRPEMEIVAINDLMDAKTNAHL
                     LKYDSVHGTYEGDVKAGDEYIEAGGRTVKVFAEKDPSKLPWGDLGVDIVIESTGVFRS
                     KEKASAHLAGGAKKVIITAPGKNEDITIVMGVNEKKYDPANHHIISNASCTTNCLAPV
                     AKVLMDSFGIRRGLMTTVHSYTNDQRILDLAHSDLRRARAAGMSMIPTTTGAASAVAL
                     VLPELKGKLNGFAMRVPTPNVSVVDFVADLEKKATAEDVNNALRAAAEGPLSGILAYT
                     QEELVSHDFNHDPHSSIVDGLCTMVIDGDMAKVIAWYDNEWGYSNRVVDLAGYIAKSL
                     "
     gene            6489..7685
                     /locus_tag="PLM74_03965"
     CDS             6489..7685
                     /locus_tag="PLM74_03965"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HON42273.1"
                     /translation="MRKLTVEDVKVTGKRVFVRVDFNVPMDADRNITDDRRIAAAVPT
                     IQYLIDQGARVILASHLGRPKGRFDPGSTMDPVADRLSALLGRPVRKLPDCVGHEVEE
                     AVAAMLPGDVVLLENVRFHKEEEANDGEFAVRLASLADLFVNDAFGAAHRAHASTEGI
                     AHYIPGVAGFLMRKEIEIMGQALADPARPFVAILGGAKVSDKIGVIDNLLTRVDTLLI
                     GGGMAFTFLKAQGHEVGKSLVDAERLDYAGQALSRAREKGVRFELPTDVVAAERFEAD
                     SPHRVVGVNAIPADWMGLDVGPDTTRRFAEIVRGAGTVVWNGPMGVFEMDAFAAGTRG
                     LAEAMASSGAVTIVGGGDSAAACEQFGLADKMTHVSTGGGASLEFLEGRVLPGVAALG
                     DKEPGV"
     gene            7692..8459
                     /gene="tpiA"
                     /locus_tag="PLM74_03970"
     CDS             7692..8459
                     /gene="tpiA"
                     /locus_tag="PLM74_03970"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017251638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HON42274.1"
                     /translation="MRSPIVAGNWKMHKTIAEARALACELVDGVSGFAGCQVVLCPPF
                     TAIHTVAGSVAGSDIEVGAQDVYWEAKGAYTGEVSCEMLIDAGCRWVIIGHSERRAMF
                     GETNDTVNRKVKAALAAGLLPIVCVGESLAQREQGITADVVSKQAEGAFMGVPADKAG
                     QVVVAYEPIWAIGTGRAATGEDANEVARAIRRTLSRLYSDRVSEAVRIQYGGSVKPSN
                     MAEFARQSEIDGALVGGASLAAEDFAGIIGATVNAKK"
     gene            8503..9798
                     /gene="eno"
                     /locus_tag="PLM74_03975"
     CDS             8503..9798
                     /gene="eno"
                     /locus_tag="PLM74_03975"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HON42275.1"
                     /translation="MSIITDVYAREILDSRGNPTVEVEVTLSDETVGRACVPSGASTG
                     QHEALELRDGDPGRYLGRGVTKAVDNVNDTIADEIVGMDARDQALVDLTLIELDGTPN
                     KSKLGANAMLGVSLAVAHAAAEYIGLPLYQYLGGVGARTLPVPLMNILNGGKHADNNV
                     DIQEFMIAPVGADSFAHALRMGAEIYHSLKAVLKKRGLSAGVGDEGGFAPNLDSNEQA
                     IAVVVEAIDKAGFAPGKDVFVALDVAASELWRDGKYVLESEGKELDAAGLVDYYENLV
                     GKYPIISIEDGLAEDDWDGWRIATLRLGDRVQLVGDDLLVTNVERIEKAIEQGVANSV
                     LIKVNQIGTLSETLDAIEMAKRAGYTAVISHRSGETEDTTIADIAVATNAGQIKSGAP
                     CRTDRVAKYNQLLRIEDLLADAGMYAGMDAFYSIKRAQR"
     gene            9825..>10041
                     /locus_tag="PLM74_03980"
     CDS             9825..>10041
                     /locus_tag="PLM74_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003386966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HON42276.1"
                     /translation="MHGITRIQGVLVGHAQNDEALTGCTVVLTPGGAVAGVDVRGSAP
                     GTRETDLMRPCNQVEKVQAVVLSGGSAY"
BASE COUNT         2118 a         2677 c         3359 g         1887 t
ORIGIN      
        1 ggcgcgttgc tgagagatgg ccgggaggtc ctcggcgaga cttcagtagg aagcagcaca
       61 tccaggatag agcgggtgtt tctgcgtccc gcaaatccca ggcccctgcc ggaggcgctg
      121 cgggcgatag cggaggccga tgctatcgtg ctgggtccgg gcagtctgta cactagcgtg
      181 atgccgaatc tgctcgtgga tcaagtggcg gaggcgatct ctgcatccga agcagtcaag
      241 atatacgtga gcaatgtcat gacagaacct ggcgagacgc aggggtacac tgcccaggat
      301 cacctggccg ccattttccg tcatgccggg ccggatctgg ttgattggat cattgtcaat
      361 gacgctccta taactgacgg aatgatgcga aaatactcat atgagggcgc cgaaccagtg
      421 atgtgggaca gggaggtcct ggaatccatg ggaatccagg tagtggaggc gccagtggca
      481 agccagactg agttggtgag gcacgacccc gatgcgatcg cagcggaggt catggctttg
      541 gttgcgtcga cgcgaggggc gagcaccgct acatgtgagt gaacctgcag atgaacacgg
      601 ccgctaagaa ccctgctagg cttgcgagca gtgccgcggg cactgtgtgg cgcgttcggg
      661 ttatcccgac agagccgaag tagagagcga tgatgtaaag agacgtatcc gtcgaaccct
      721 gtatgacaga tgccagtaat ccctcagccg agtccggccc gtaagctgcc agaagcttgg
      781 ccaagtatcc cagagctcct gaacctgata tgggtcgaag cgcggcaagg atcaagagat
      841 cagggtgtat tccgagggcg cgcgccgcag gcgagagggc gcgcgccgct gcatctaggg
      901 ctcctgaatc gcggaacatc cccacagcga cgaacatggc gatcatcgcc ggcgcaatgc
      961 gcgctattgt tctcaagccg tccgatgcgc cggcgatgaa gcagctatat acgtcaatac
     1021 ccttcatggc gccgtacacg ggtattccca caagcagaag aagcacaaca taataggaca
     1081 gggacgtgag ctgtctcatc ggttctttcg acctctcttc ccgagggcga agtcgacgat
     1141 tactgcaacc gcggtggaac atgcggtcgc gacaattgtg gctccgatga cgccggccgg
     1201 gtcagccgat cccatctcgg ctctgagcga tatgatcgtc gtagggacca gcgtgaggct
     1261 ggaggcagtg atgatgacga aggtgcacat ggcgtcactg ggctctgact gggtgcggct
     1321 ttcgattgcc agttgctgca tggcggagag cccgagtggg gttgcagcca tgcctagccc
     1381 gaggaggttc gcagccatgc tcgcggcgat gagtggcgtt gacttggact taggctctag
     1441 ctcggggaag agcgggccaa tgagcggccg gattgctgag gccagcgcag ttgtgagccc
     1501 ggactgctgc gctatttggg ccatgcccga ccaaagacct attgagcccg ccaatgccag
     1561 gcagagctgc acagcttctt ttgtcgagga gatggccatc gccgtaacgg cctgaggccc
     1621 acgggcgaca gctgaccaga tcgtgccgat cgccaacatc gcgacccaga taatgcccat
     1681 catgcctttt cctccacaca ttggcccggc caatttcgac ttgcgccgcc gctccatcgt
     1741 attcatgccg tccgggcgag tatgcgctat gctcactcac gcagatgaag ggaatcgcca
     1801 agttggcggg gaatagagtc agtgctgagc ccggggagtg atttccgaat ggacttttac
     1861 tcgctgatga aggatgaact agttcgggcc atgcccgagc gtggttgctg tcgcgtggcg
     1921 gagctatcgg cgctgctcat ggctgacggt gtgatccgga cctcagaagg acatgtgagc
     1981 atagtgatct ctcagaccca cgctggcgtg gcgcgaaaag tagtctcact cattcgtgat
     2041 ctggagtgga aggctctgga gcgccctggc gtggaagtcg ccgtccgata tcgaaagagg
     2101 aagggcgccc cccgcggcaa cctgtacgtg gtccggatag cgaaccatga tgaagccatc
     2161 gatctgctgg accgcctggg cctgggcctg ggcctggccg ggccggatgg ccttgtgcac
     2221 tacgcggagc gtcaagttct cgccaggcgt tgctgcaaac gggcgtacat tcgcggcgtc
     2281 ttcctgggag caggctacat gaccgacccg tcgaaaggat accatctgga gatagcagtt
     2341 cgaggcctgg agaatgcgga gtacatttcg ggtcttcttg ccgagttcgg gattcgcgca
     2401 gggatagtgc cacgtcgagg gcgaagcgtg gtgtatctca aagacggcga cgatatcgtg
     2461 gaattgctcc gcgtgatgca ggcgtccagc gcgcttctgg agcttgagaa cgcgcgaata
     2521 ctgcgatcga tccgcggcga tgtcaaccgc ctggtcaacg ccgaaaacgc caacctggcc
     2581 aagactgtgg acgcctcttt aggtcagata cgcgatattc agctcataga cgatgtgatg
     2641 ggcatcagga ggcttccccc gactcttgcc gcagcggcgc gcctcaggct tgagaacccg
     2701 gaagccagcc ttacagagct cggagccatg ctatcgccgc ctgtgagcaa atcgggcgtt
     2761 aaccaccgca tgcgcaggat tgcccgaata gctgacgaca ttcgcttgca gagcaggcgc
     2821 gagaggtgat gccccgtgga aatgtcctac gggatggttc agcagcaagt ccagaagctg
     2881 gtgatgactc cccagttacg ccaggctatc cagcttcttc aattgccgac cgtcgatctg
     2941 gcgcagtacg tccaaaatga gctgctcgga aacccggttc tggaagaagc cgatcagccg
     3001 gccgaagggt cggccgatga gaaccaggcg tctcgcgccg acgatgacca gatagactgg
     3061 gcaggctatt tcgccggcga tggaggcgcc gattctggtt ggccttcgga tttgtcagat
     3121 cgggacagtg atgacgatcc ggtcaggctc atcagatggg agccttcgct tgcagagcgc
     3181 ctgcacgtag agctaagggt agcgtcgact tgccccagga ctctatgtat cggcgagtat
     3241 ctcctcggat gcattgacga cagcggcttc ttccgcggag acgtagctga tgtggcggcc
     3301 gccctaggag tgccggagcc tgaggtggaa gaggtgcttc atctgatcca gtgcctggat
     3361 cccgtgggcg tagggtcgcg aagcgtccgg gaatgccttg agctgcagat atctgccaag
     3421 tcggctcccg atgacatgcg cgcgctggct atgcgaatcg ttcgagaata cctggacgac
     3481 ctggccgaag gcaggatatt gcgaatcgcc tctgagcttg gcgcagatgt cgcgcaggtt
     3541 caagaggccg ccgatatgat acgcgctctc tctccccgcc cggggcagga ctacgcgggg
     3601 ggcggacaag ctcggtatat cgttcccgat gtgatagtcg aaaaggtagg ggacgattac
     3661 gttgtgacat cgtgcgattc ggcatcgccc cggttggtaa taaacagcgc ataccgagcg
     3721 atgctgtctg atgcgcgtac tgatacggcc gccagggatt tcatacgtga acgccttaat
     3781 tcagctgcgt ggctcatacg cgcccttggt cagcgtcgcg acaccatcac caaggtcgcg
     3841 acttccatcg tcgcccatca acgggacttc ttcgaccgag gtattctcca tctgcgtcca
     3901 ctaacgctta gggaagtggc tgttgacatc gatatgcacg agtcgaccgt cagtcgggcc
     3961 actgcaggga agtacgcgca gaccccccgc ggcacgttcg aacttcgctt cttcttcggt
     4021 tccggggtga gcaccttagg cggcgatact gcatcggctg ccagcataaa gaagtacatt
     4081 aaggagatga tcgcctcgga agacgccaaa tcgccgctat ccgatcaaga gatagccgac
     4141 gctttgactg gcgagggcat ctgtatttcc aggaggacag tagcaaaata caggggcgag
     4201 ctggccatac ccgcgtcgac gcgtcggagg aggtactgag cgctactgcg ctgcggcggc
     4261 cgagtcagag gatgaactga gtccaggagg aacttggccc tacgcgcaga agatgttata
     4321 ctgtaacggg tgggacagga attggctatg cgggacgcaa agcgtccagc taagggagac
     4381 cgccatggga aagacggctg acataatcgc tgtgcagtgt cggatagcgc cggagcttac
     4441 tgaaagaatc gtgctccggc acaacatcct gaggcacgta tcgtattcgc agcctatcgg
     4501 gcgcagagcg cttgcccagt gcatagggag aagcgagcgc gcggtcaggc gagaggagaa
     4561 cgcgcttcgg gaggtcggtc tcatcctcgt gggctcggat ggcatccgcc tctccagaga
     4621 tggggaggat atcctgtggg aactgggaga gtatgttcgc ctgctcaccg gcaccgggga
     4681 acttgagagg acgctggcgg ggagattcgg cctcgagcaa gccatagttg tgaccggcga
     4741 ctccgaccgc gatgacgcgg tgaagagaga gattgcgcgt gcaggcgcga gcgtgctcag
     4801 ggacgtaatc tccgatggcg acgtggtagc tgtgaccggc ggtactacaa tggcggctct
     4861 ggcccaggag gcggtggagg tacgctccgt ccgagatctt gtggtggttc ccgcccgggg
     4921 cgggctagga gaggatgtcg aaaagcaggc cgacacggtt gctgcgtccc tcgccaagaa
     4981 gttgggaggc agttacaggc ttctcaacct ccctgacgac ctcgggctcg agtctgctgc
     5041 aagtgttcgc gaagagcccc ggatcaagca ggtgctcgac acgatccggc aggcgcgtgt
     5101 ggttgtgcat ggcgtgggca cggccctgga aatggcgaag aggaggaacc tctccgaggc
     5161 cgagatcgac cacatcaagc agctaggagc tgtgggcgaa tcgttcgggt tctacttcaa
     5221 ctccgccggc gaggtcgtat acaccacgtc cagcctgggc ctaaggcttg ccgacctgtc
     5281 gtcagtccct acggtgatat gcgtgggcgg cggggccagc aaggcacagg cgctgtcggc
     5341 ggtgttgcgt aacgggtttt ctcacatcgt tgtcacggat gaaggagcgg cgcgcgagat
     5401 caatcgcgaa caggggaggt gaaccccgcc ggaggatcgg cgggagccaa tggcagtcaa
     5461 gattggaatt aatggttttg gaagaatcgg acggctcgtg ttcagggcgg gcgaaaagcg
     5521 tccggaaatg gaaatagtgg ccataaacga cctgatggat gccaagacca atgctcatct
     5581 tctgaagtac gattcggtcc atggtacata cgaaggcgac gtgaaggcag gcgacgagta
     5641 catagaagcc ggcggtagaa ccgtcaaggt ctttgcggag aaggacccgt ccaagttgcc
     5701 atggggcgat ctgggcgtgg acattgtgat cgaatcgact ggggttttcc ggtccaagga
     5761 gaaggcatcg gcgcaccttg ccggcggcgc aaagaaagtc atcattacgg cgccgggcaa
     5821 gaacgaggac ataacaatcg ttatgggtgt caacgagaag aaatacgacc cggcaaacca
     5881 ccatatcatc tccaacgctt catgcaccac taactgtctt gcgcctgtgg ccaaggttct
     5941 catggattcg ttcggcatcc ggcgcgggct catgactact gtccactcgt acactaacga
     6001 ccagcgaatc ctggacctgg ctcactcgga tctcaggcgg gcgcgggctg caggcatgtc
     6061 catgataccc accacgacgg gagcagcgtc tgcggtggcg cttgtattgc ccgagctcaa
     6121 aggcaagctg aacgggtttg ccatgagggt gcccacgccc aacgtgtcag tggtggattt
     6181 cgtcgccgac cttgagaaga aggcgacagc ggaagatgtg aacaacgcgc tccgcgcggc
     6241 ggcagaaggt ccgctttcgg gcatccttgc ctacacgcaa gaagaactgg tgtcacacga
     6301 cttcaaccat gatccccatt cttccatcgt agacggactg tgcaccatgg ttatcgacgg
     6361 agacatggcc aaggtgatag cctggtacga caacgagtgg ggatactcca accgcgtggt
     6421 cgacttggca ggctacatcg cgaagtccct ttagccaaag tcgcgcggcg gagaggagct
     6481 gattggcatt gcgcaagctc acagtggaag atgtaaaagt aaccggcaag cgcgtattcg
     6541 tgcgagtcga tttcaacgtg ccgatggacg ctgaccggaa cataaccgac gacaggagaa
     6601 tcgctgcggc ggttccgacc atacagtacc taatcgatca gggggcgagg gtgatactcg
     6661 cctctcacct cgggcgcccc aagggcagat tcgatcccgg atccacaatg gatcctgttg
     6721 cggatcgtct ttcggcgctc ctgggtcggc cggtgcgcaa gctccctgac tgtgtggggc
     6781 atgaggtcga ggaggccgtg gccgccatgt tgccggggga tgtggttctc ctcgaaaacg
     6841 tgaggttcca caaggaagag gaggctaacg acggggagtt cgcggtgcgt ctggcatcgc
     6901 tggccgacct gttcgtcaac gacgcattcg gcgcagcgca cagggcgcat gcttcaacgg
     6961 aaggaatagc ccactacata cctggcgtgg ccggattcct aatgcgcaag gaaatagaga
     7021 tcatggggca ggctctggcc gatcccgctc ggccgttcgt cgcgatactc ggaggggcta
     7081 aggtatcgga caagatcggc gtcatcgaca acttgcttac tcgcgtggac acgctcctca
     7141 ttggaggcgg catggccttc acgttcctga aggctcaagg gcatgaggta ggtaagtcgt
     7201 tggtagatgc ggagaggctg gattatgccg ggcaagcctt gagtcgcgcg agggagaagg
     7261 gggttcggtt cgagcttccc acggacgtgg ttgcggcaga gcggttcgaa gctgactcgc
     7321 cgcatcgggt tgtcggggtc aacgccatac ctgctgattg gatgggcctg gatgttggtc
     7381 ccgatactac tcggcggttc gccgagatag tacgcggcgc aggcactgtg gtgtggaatg
     7441 gtccgatggg agtgttcgag atggatgctt tcgccgcagg cacacgcggc ctggccgagg
     7501 cgatggcatc gtcgggcgcc gtaactattg tgggaggcgg cgactccgcg gcggcctgcg
     7561 aacagtttgg cctggccgac aagatgacac atgtatcgac aggcggcggc gcgtccctcg
     7621 aatttttgga gggacgcgta ttgccgggcg ttgcggcgct gggggataag gaaccggggg
     7681 tgtaggaatc tgtgaggagt ccaatagtag caggcaactg gaaaatgcat aagacgatcg
     7741 ctgaggctcg cgcgctcgcg tgtgagctgg tcgatggggt ttcggggttt gcaggatgcc
     7801 aggtcgtgtt gtgtccgccg ttcacggcaa ttcacacggt ggcagggagc gttgcaggat
     7861 ccgacatcga agtgggagcg caagacgtgt attgggaagc caagggcgcg tacacaggcg
     7921 aggtctcctg cgagatgctg atcgatgccg gatgccgctg ggtgatcatt gggcattctg
     7981 agagaagggc tatgttcggc gaaacgaacg acacagtcaa tcgtaaggtg aaggcggcat
     8041 tggcggcggg tctgctgccg atagtatgcg tgggagaaag cctcgcccag cgcgagcagg
     8101 gcattacggc cgacgtagtg agtaagcaag cagaaggggc tttcatgggt gtgcctgccg
     8161 acaaggccgg ccaagtcgta gtggcgtacg aacccatctg ggccattggc acgggacgcg
     8221 ccgctacagg tgaagacgcc aacgaggtcg ccagggctat ccggcgtacg ctgtcgaggt
     8281 tgtactctga tcgggtgagc gaggcagtga ggatccagta tggaggaagc gtaaagcctt
     8341 ccaacatggc cgagttcgcc aggcagtcgg agatagatgg tgcgctggtg ggcggggcga
     8401 gtcttgcggc agaggacttc gcggggatca ttggcgcaac ggtgaatgcc aagaagtgag
     8461 caggtgagaa aggagcacag gcggcgtgtg ccgccgacgg aaatgtcgat aatcactgat
     8521 gtgtatgcgc gcgagatact cgactccagg ggaaacccca ctgtcgaggt ggaggttacg
     8581 ctctccgacg agacggtggg cagggcatgc gtgccttcgg gtgcctctac cggccagcat
     8641 gaagcgcttg agctgcgcga cggagacccc ggtcgctacc taggccgagg cgtgacgaag
     8701 gccgtagaca atgttaacga cacgatcgct gatgagatcg tgggcatgga tgcccgagac
     8761 caggcgctag tcgacctgac actgatagag ttggacggga cgcctaacaa gtcaaagcta
     8821 ggcgccaacg ccatgctcgg agtgtcgctg gccgtggcgc acgctgcagc cgaatacata
     8881 ggtctaccgc tttatcagta tctgggaggc gtaggcgccc gcacactgcc ggtgccgcta
     8941 atgaacatcc tgaacggcgg caaacatgcc gacaacaacg tggacataca ggaattcatg
     9001 atcgcgcctg tgggggccga cagtttcgcg cacgccctta ggatgggggc tgagatatac
     9061 cattcgctca aggccgtgct gaagaagcgc gggctctccg caggtgtggg agacgaggga
     9121 ggatttgcgc ccaacctcga ttcgaatgag caagccatag ctgtagtggt cgaggccata
     9181 gataaggccg gtttcgcgcc cggtaaagac gtgttcgttg cgcttgacgt agctgcaagc
     9241 gagctatggc gggacggcaa gtacgtgctc gaaagcgaag gcaaggagct tgatgctgcg
     9301 ggtctggtgg actactacga gaacctggtc ggcaagtacc ccattatctc catcgaggat
     9361 gggctggcag aggatgactg ggatggctgg cgcatcgcta ctctgaggct gggcgaccgg
     9421 gttcagctcg tgggcgacga tctcctcgtc acgaacgtgg agcggattga gaaggcgatc
     9481 gaacagggtg tggccaactc tgtgttgata aaggtgaacc agatcggcac gctctccgag
     9541 acgctggacg ccatcgaaat ggccaagcga gccggctaca cggcggtgat ctcgcaccgg
     9601 tcgggcgaga ccgaagacac taccatcgcc gacatcgcgg tggccacgaa tgcgggccag
     9661 atcaaatcgg gcgccccgtg ccgtacggac cgggtggcca agtacaacca gctcctccgt
     9721 atagaggatc tcctggccga tgcaggcatg tatgccggca tggacgcgtt ctactcgatc
     9781 aaacgcgcgc agcgctagca atgcgcgagt gggggcgatg agtcgtgcac ggcataactc
     9841 gaatccaggg cgtgctcgtg gggcacgctc agaatgatga ggccctaacc ggttgcacag
     9901 tggtgttgac tccgggaggg gcggttgcgg gcgtggacgt aaggggatca gcccccggta
     9961 cgcgcgagac cgatctcatg cgtccctgta accaagttga gaaggtccag gccgtggtgc
    10021 tttcaggagg aagcgcgtac g
//
LOCUS       DAOTAC010000061         9891 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_5831_length_9891_cov_4.298699, whole genome shotgun sequence.
ACCESSION   DAOTAC010000061 DAOTAC010000000
VERSION     DAOTAC010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 9891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9891
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5831_length_9891_cov_4.298699"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..1293
                     /locus_tag="PLM74_03985"
     CDS             103..1293
                     /locus_tag="PLM74_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HON42277.1"
                     /translation="MNTRDKHFETLSIHAGGEDRNPKNALNYPIFMTSTFEFDSVEHA
                     DATFGFETEDYVYTRGNNPTLKLLEERMAVLEGGAASVAFASGMAAISSVLMSLLKPG
                     DEVIAHRVLYGSAWNLIKNILPQYGVSSRMVNLVGIGDLADQITERTKVIYFETPTNP
                     NLDIIDIAAVCDIARAKGARVVVDNTFATPFFQRPLKLGADVVVHSATKYISGHGDVI
                     AGIAVAKDRDYAGELKFGYMCEFGGVMSPFTGWLLLRGLKTLALRMREHEKNALAVAR
                     YLEQHPRVKRVYYPGLPSSPGHETAKAQMSGFGGMVSFDIDGTLAQSKQVVNSMRLFK
                     LAVSLGDCESLVEHPFAMTHRGYTEDEIYAAGLTPSMIRLSIGLEHQDDLIADLEQAL
                     AAIG"
     gene            complement(1353..2783)
                     /locus_tag="PLM74_03990"
     CDS             complement(1353..2783)
                     /locus_tag="PLM74_03990"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HON42278.1"
                     /translation="MRNPDSSVASLLPFDPSYTVAQVRDLLTRGANVNETINDGWTPL
                     MFAVHRGAGLEIVNALIEAGANVNAADDEGITALMLASMRAPDPVVAQALIHAGANVN
                     AATDDGFAALMFAARHNPCPGVAKVLLSEGAAVDTATDTGSTALMFASMNTAAAAVLE
                     PLLAAGANPNAVDGTGWAPLFMAAANTDDPKVIDALLEAGADPNALDEAGNTPLMIAL
                     TYTRSLAIIDILAGLDNDVNYCTPCGIGPMQLAAQRDDSELVKAILCQAGAKLREDFA
                     PELGPSISAEAKAASYCPNGDANVRDEHGGSPLIRAIADDADPEVVAALLEAGADVHA
                     GESDDGRTPLAYAAMSASDPRVIRMLLDSGSNIHVRYEDDYTVLMLAAGDNHNPEIIR
                     MLVAAGVDINAVKQPEGWTALMIAAQAGTPHAIIEALLDAGADGTLRCAAGKAAFDYA
                     RGRKLLKGTDVYWRLRDAKYQPPAPR"
     gene            complement(3272..3631)
                     /locus_tag="PLM74_03995"
     CDS             complement(3272..3631)
                     /locus_tag="PLM74_03995"
                     /inference="COORDINATES: protein motif:HMM:TIGR01764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HON42279.1"
                     /translation="MEINKEWLSVQEAADYLGVARPTIYRWAKESKIRIFKLAKGVAR
                     VKRQDLETFLQQAPVLYSEPADHGDHATQPGFVRETARQQSQTADPILDVLGSLSGEP
                     ISAEEIEKELHGNEEMS"
     gene            complement(3730..4380)
                     /locus_tag="PLM74_04000"
     CDS             complement(3730..4380)
                     /locus_tag="PLM74_04000"
                     /inference="COORDINATES: protein motif:HMM:NF017747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutD family protein"
                     /protein_id="HON42280.1"
                     /translation="MSADIQVTRRSQHRTSAWSGGETTELAIFPPNSNYAARNFEWRV
                     SSATVTSETSEFTRLPGYSRRLLVLAGELRLQSADNHETILHPGEQCSFRGGMPITSY
                     GLATDFNLMLGPGWEGSLTHVILAPGESRTWHPADTAQHALIYCRQGQVSITPANARA
                     QNLDAGDLMWAISDARCLESFDIVGEGKGQSTLVLVTVADAQKSPDKSSLATLMTI"
     gene            4610..5836
                     /locus_tag="PLM74_04005"
     CDS             4610..5836
                     /locus_tag="PLM74_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HON42281.1"
                     /translation="MVDANSTRPRARDIGIDIGVFRPGRWNAITDALGVVVGHVTLVE
                     GDGGLAPGRGPIRTGVTVILPHSGNLFEEKVPAAAYVLNGFGKSIGLHQVNELGNLET
                     PIALTNTLNAPLVADALIEWSIKSNPQIGIGTGTVNPVVGEVNDGSLNDIQGRHVRKQ
                     HVFEAIVKARSGAVAEGSVGAGTGSICMGWKGGIGTSSRVLPPRRGGYTVGVLVQTNF
                     GGALTVNGAPVGRELGRYPNSADFPYETPGTGDVEGADPEGIDLEIRVREVDDPDGSV
                     MVIVATDAPLDQRQLERLAKRAGLGLARTGFYSSNGSGDFFIAFSTAARIPHGSPLTA
                     KAEVVSNDAMSPLFLAVLEATEEAVINSMLRATTVIGRDGRTVDAIDIDQLLRVMGKY
                     NALNWSRVLPPWGSME"
     gene            6297..8516
                     /locus_tag="PLM74_04010"
     CDS             6297..8516
                     /locus_tag="PLM74_04010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HON42282.1"
                     /translation="MEPVMEARTESVPVCGGATLATSITLPGPGAYPVVIVRTIYGRR
                     NLQSIGDLLANHGYATVIQDVRGSGESSGQFGFLAQEPKDAVQTAQWVLDQPWCDGRL
                     AILGISYLSAASIAIAAEFPDEVKAAVWVTVPVRKDLLCFQDGALRLHHTLPWTYMRR
                     ASLSSFNPEALYRKTPLIDAIPQSVDSSWAAMLDEGPRSGFWDGNDLWSYVQRTCVPG
                     LHFGGWFDFLLDATLVPYSEMVQSGAPQRLVLGPWSHNGTIGGPERISGDVSYGYWSG
                     QREGGLDGLHEDPVDMSSPFMEECVSWLDFWVKGAGDSPPRSSVKCFFTGHEPHWAEL
                     GAWPHPRASGVRMDLGAAAGGQSEAGSRGRGVLSAFSPVFSADGAFGTNPVAASESFV
                     YDPDDPVSTEGGAVWAFPKAGMAPGPSVSSAHLREDVLTYDSEVLGSPLEICGAAEVE
                     LHASSDAPDTDFVAMLLDVDEDGVARYVSDGIVRARYRNSLDREDFLEPGQIYLFRIR
                     LKGCAHTFGPGHRVRLTVTSSNFPKFDRNPNTGEPIYRSRRMQKAVQTVYCGPALPSA
                     VVIPVLSGYQVRSLHDDDREWAERLLKESWGSANIVTRGRLVDASRLPGFVAVAGSGD
                     RLGLLTYEERNGQCEIVTLNSLRRGMGIGQGLIWRVVECAKEAGASRVWLITTNDNTD
                     ALRFYQKAGFRIVAVHQDAVALARRIKPEIPIMGENGIPIRDEIELELMLDTDHGNV"
     gene            complement(8562..9047)
                     /locus_tag="PLM74_04015"
     CDS             complement(8562..9047)
                     /locus_tag="PLM74_04015"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HON42283.1"
                     /translation="MSIRCFVDTSAWFALLMAADQYHAAVRAEYALAHSEGGIFITSS
                     LVLGELHTLLLARTGTAAGFWSFRDDIALSGQVRVLHPTTNQLDMAFDLLRRRPDKSY
                     SFVDATSFVMMRDESIHTALSLDRHFAQEGFAVIPVPEECLHEPPQAYPAAPTSAGEG
                     S"
     gene            complement(9044..9310)
                     /locus_tag="PLM74_04020"
     CDS             complement(9044..9310)
                     /locus_tag="PLM74_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HON42284.1"
                     /translation="MPDRSRMHRMQFYIEPQVHDELSLLARRRNVAMAELIREAIASF
                     IARETPLQDDPALRIIGMIDSPDAPGDAAVNHDRYIYRKDWEDR"
BASE COUNT         1967 a         2737 c         3075 g         2112 t
ORIGIN      
        1 tttggcgcac gcccgatgct gcgagctggc aatcaggcag tcgggcggat caccggcttt
       61 gcgaatcaac tcggcgaatg ccaggagaga tgagagaatc cgatgaacac tagagataag
      121 cattttgaga cgctgtccat tcacgccggt ggcgaggata ggaatcccaa gaatgcgctg
      181 aactatccta tctttatgac ctccaccttt gagtttgatt ctgtggagca tgccgatgcc
      241 acgtttgggt ttgagactga agactacgtg tacacccgcg gcaacaaccc taccctcaaa
      301 ctgctcgaag aacgtatggc ggtactggag ggaggcgcag cctcggtggc ttttgcttcg
      361 ggcatggcgg ccatcagctc ggtactgatg tcgctgctca aacctggcga tgaggtcatt
      421 gctcacaggg tgctgtacgg ctcggcctgg aatctcatca agaacatatt gcctcagtat
      481 ggtgtgagct cgcggatggt caaccttgtg ggcatcggcg atcttgcgga tcagattacc
      541 gaaagaacca aggtcattta cttcgaaacg ccgaccaatc ccaacctgga catcatcgac
      601 atcgccgctg tatgcgacat cgctcgggcc aagggagcga gagtagtggt cgacaatacg
      661 tttgctacgc cattctttca gcgccccctg aagctcgggg ctgatgtagt agtgcacagc
      721 gccaccaagt acataagcgg gcacggtgat gtcatcgcag gcatcgctgt ggccaaggac
      781 agggattacg ccggcgagct caaattcggt tatatgtgcg aattcggcgg agtcatgagc
      841 ccattcaccg gctggctgct gctgcggggc ctgaagactc tggcgctcag gatgcgcgag
      901 catgagaaga acgctctggc agtggccagg tatcttgagc agcatcccag ggtgaagagg
      961 gtgtactacc ccggcttgcc cagttcgccc gggcatgaga ctgcaaaggc ccagatgagc
     1021 ggattcggcg ggatggtcag cttcgatatc gatggaacgc tggcccagtc caaacaggtg
     1081 gtgaacagca tgagactgtt caagctggct gtaagcctgg gcgactgcga gagcctagta
     1141 gagcatccct ttgcgatgac ccacagaggg tataccgagg acgagatcta cgccgcgggt
     1201 ctgactccca gcatgatcag gctatctatc ggcctggaac accaggacga cctgatcgcc
     1261 gacctcgagc aggcgctcgc ggcgatcgga taagacagta aacagtaagt tgagcgcagt
     1321 gcattatcgg ccacgctatt cgcaactctg gcttaccttg gcgctggagg ttggtacttt
     1381 gcatcgcgca gtcgccagta cacatcggtg cctttgagta acttccttcc cctggcgtaa
     1441 tcgaaggcgg ccttccccgc tgcgcatctg agcgtgccgt ctgcgcctgc gtcaagcagc
     1501 gcttctatga tggcgtgtgg cgtaccggct tgggctgcaa tcatcagagc agtccagccc
     1561 tctggctgct taactgcgtt gatgtctaca cctgcagcaa caagcatacg gatgatctcg
     1621 gggttgtggt tgtctccagc ggccagcata agaaccgtgt agtcgtcttc atagcgcacg
     1681 tgaatgttgc tacccgaatc gaggagcatc ctgatcactc ggggatcgga agcactcata
     1741 gctgcatagg ccagaggcgt ccgcccgtcg tcggactcac cggcatggac gtcagcgcct
     1801 gcctcaagta atgccgccac cacttcagga tcggcgtcgt ctgctatggc gcgtatgagc
     1861 ggtgagccgc catgctcgtc gcggacgtta gcgtcgccgt ttgggcagta gcttgcggct
     1921 ttcgcctctg cggagatgct cgggcctagt tcgggagcga aatcctcacg aagcttcgcg
     1981 ccggcctggc agagaatcgc tttgaccagt tctgaatcat cgcgttgcgc agccagctgc
     2041 atgggaccga ttccgcacgg cgtgcagtag ttgacgtcgt tgtcgagccc ggcgaggata
     2101 tcgatgatcg ccaggctgcg ggtgtaggtc agcgcgatca ttagcggtgt gtttcccgcc
     2161 tcgtcgaggg cgtttgggtc ggctcccgcc tccagaaggg cgtcgattac cttgggatca
     2221 tctgtgtttg cggcagccat gaacaacggt gcccagccgg tgccgtccac tgcattcgga
     2281 ttcgctccgg cggccagcaa cggttccaaa accgcggcgg cggccgtgtt cattgacgcg
     2341 aacatcagcg ctgttgaccc tgtatcggta gccgtatcca ctgcagctcc ttcgctcagg
     2401 aggacctttg cgacaccagg acacggattg tgacgagcgg cgaacatcag ggcggcgaaa
     2461 ccgtcgtcgg ttgcagcatt gacattggcc ccggcatgta tcagcgcttg ggctaccacc
     2521 gggtcaggcg ccctcatgga agcgagcatt agggctgtta tgccctcgtc gtcggcggca
     2581 ttcacattgg cgcctgcctc gattagagcg ttgacgatct caaggccagc gccgcggtga
     2641 actgcgaaca tgagaggcgt ccagccgtca ttgattgtct cgttgacgtt agccccgcgg
     2701 gttaggagat cccgtacctg agctactgtg tagctaggat cgaagggtag aagagaggcg
     2761 acgctcgaat cgggatttct gatggggaat acctccttcc aaacaggcgg gtgttgggcg
     2821 ggataggcaa cattgtcatc tctgttgagt attcgccggt tggtgtcgat ggaccttcag
     2881 aaccgcaatc tcgaaccgtt ggcgtctatt ctcatcagca ttcttcgccc cctatccacc
     2941 tagcttcacc tgggcaaaca aggatacact agcatttcgc gttgcaaggt gatcgaaggc
     3001 gatgtgccgg agtgacccgc tcaatgatga tctgccccga gctggctgct tcaatgatcg
     3061 cattgataaa acgggtagct tctgcgaaac tctccctacg aaagatcagg gtgatcgctt
     3121 cgtcaagcac atggtcgctt gtatagatac gctcacctgt gcggctgagg gcggcgtaat
     3181 actcagctgc ttcggcatgc cgcggatcct tacggcgccc gagggcaatc cagccccatg
     3241 tatctatgaa ggcaggccca cacactatga cttagctcat ctcctcattg ccgtgcagtt
     3301 ccttctcaat ttcctcagcc gaaatcggct ctccggaaag ggaaccgagg acgtctagta
     3361 tcgggtctgc tgtttgtgac tgctgccggg cagtctcgcg cacaaagcct ggctgggtag
     3421 catggtctcc atgatctgct ggttctgagt acaacactgg agcctgctgc aggaaagtct
     3481 ccaggtcctg gcgtttcacc ctagcgaccc ccttcgcaag cttgaatatg cggattttcg
     3541 attcctttgc ccagcggtat atggtaggac gtgccactcc aaggtagtcg gccgcttctt
     3601 gtacagacaa ccattctttg tttatctcca tgagagcacc tcctatgcag cgtagttacg
     3661 ggggcgctcc tgtgccggtg cacatgaact cgcgcacgga tcgccagcct ccctttgatg
     3721 cataacgtat catattgtca taagcgttgc gagggaagac ttgtcggggc tcttctgcgc
     3781 atcagcgacc gtcacaagta ccaatgttga ctggcctttg ccttcaccga ctatgtcgaa
     3841 ggactcgagg caccttgcat cggatatcgc ccacatcaaa tcccctgcgt ccaggttctg
     3901 agctcgcgca ttcgcaggcg ttattgagac ctgtccttgc cggcaataga ttagggcgtg
     3961 ttgcgcggta tcggcgggat gccaggtgcg actctctccc ggcgcgagga tgacatgggt
     4021 gagacttccc tcccagcctg ggccaagcat caagttaaag tccgttgcca gcccgtagct
     4081 agttatgggc atgccgcctc gaaacgagca ctgctcgcct ggatgcagta tcgtttcgtg
     4141 gttgtcagcc gactgaaggc ggagttcgcc ggccagcacg agcaggcgcc tactatagcc
     4201 cggcaggcgt gtgaattcac tggtttcgga cgtcactgtg gctgagctaa cgcgccactc
     4261 gaaattccgc gccgcataat tgctgttggg agggaatatg gccagctccg tcgtttcgcc
     4321 tccgctccag gcgctcgtgc ggtgttggga tcgccgagtc acctgtatgt ctgcagacat
     4381 gctatggcct cccgaactga actgtagtca ccgtaatggg gtcgcaactg ctggatctgg
     4441 acccatatcc ggatctggat cgggatgagt attcgacaac cacgcgaaag acgccttcgt
     4501 cgcaaaggac atcactggac gcagcgatcc aaggtgacct ggcaggaaag gcgaatatgt
     4561 atgggcaaga aacgcagaaa ggcgaaagac agggaggcga gcagcggaca tggtggatgc
     4621 gaatagcact cggccccgtg cccgggatat tgggatagat atcggggtat tcagaccggg
     4681 tcggtggaat gccatcaccg acgctctggg agtcgtggta ggccacgtta cattagtgga
     4741 aggggacggg ggactggccc cgggacgcgg ccccatcagg accggggtga ctgtgattct
     4801 cccgcacagc ggcaatctgt ttgaggagaa ggtcccggcc gcggcgtatg tcctcaacgg
     4861 gttcggcaag tcaatcggcc tacatcaggt gaatgagctg ggcaacctgg agacgcccat
     4921 agcgctcacc aacaccctga acgctccgct cgtggcagac gcgttgatcg agtggtccat
     4981 caagagcaat ccccagatag gcatcggaac ggggactgtc aaccccgtcg tcggagaggt
     5041 caacgacggc agtctcaatg acatccaggg ccgccacgtt cggaaacagc acgtgttcga
     5101 ggcgatagtc aaggccaggt ctggcgctgt tgccgaaggg tcggtcggag caggcacggg
     5161 aagcatctgt atgggatgga aaggcgggat aggcacgtcg tctcgcgtgc ttccgcccag
     5221 gcgcggcggc tacaccgtcg gggtgctggt tcagaccaat ttcggcggcg cgctcacagt
     5281 aaacggggca cctgtcggac gcgaactggg gcgctacccg aacagtgcgg attttcccta
     5341 tgagacgcct gggactgggg atgttgaggg ggcagatccc gagggcatag atctcgagat
     5401 tagggtccgc gaggtcgacg atcccgacgg atccgtgatg gtcatcgtgg ccaccgatgc
     5461 ccctctcgat caaaggcagc tggagcgact ggcgaagcga gccggccttg ggctcgcccg
     5521 caccggcttc tacagcagca acggctcggg ggacttcttc atagccttct ccaccgccgc
     5581 gcgcattcca catggtagcc ctctgaccgc caaggccgag gtggtgtcga acgacgcgat
     5641 gtctcccttg ttcttggcgg tgctcgaggc caccgaggag gccgtcatca actcgatgct
     5701 tagggccact acggtcatcg ggcgcgacgg cagaaccgtt gacgctatcg atatcgatca
     5761 gctgctcaga gtcatgggga agtacaatgc gctgaactgg agtagggtgc taccaccgtg
     5821 gggaagcatg gagtagatcg tctcgcaggc aattcggcct gtcaacattg aaccggcatt
     5881 cgggcgcgtg tagcgattgt tctccttgcg cctctggagc gttatcttcg tggcaacgcg
     5941 ttaagttgcg gccgaagcaa gtgtgtgctc ttcggcccaa aaaccgagta aatgccagct
     6001 cgggtcgttc tgatgcccat cgtcactcat taggccagtt gggcgccatt tttgcagagc
     6061 gtggcagctt ctttcctgta tccacctgga gatacccaac tcgcgttccg tttcggtcgc
     6121 tgatcgacag ccttctgaag atccgtgtga caggctgccc catgaaccaa gcacacaact
     6181 gcgcctcata ccttagtcaa gcagcgccgc ccggcgaagc aggcatgtgg cgccaagcgg
     6241 tgaatctcaa ccagagtatg cagtgcgccg aggtacatcc gagggaggag ccagctatgg
     6301 agccagttat ggaagccagg actgaatctg tgccggtgtg tggtggagcg acccttgcga
     6361 catctatcac cctgccgggc cccggggcct accctgtggt cattgtgcgg acgatctatg
     6421 ggcgtcggaa tctgcagagc atcggcgatc tgctggcgaa tcacggctac gccactgtga
     6481 tccaggacgt gcgtgggagc ggcgagtcgt cggggcagtt cggattctta gctcaggaac
     6541 ctaaggacgc tgttcaaacc gcacaatggg tgctggacca accatggtgt gacgggaggc
     6601 tcgccatact cgggatctct tatctttcgg ccgcttcgat tgccattgcg gccgagtttc
     6661 ccgacgaggt gaaagcggcg gtctgggtta cggtgcctgt caggaaggat ctcctttgct
     6721 tccaggacgg agcactccgt cttcaccata ccttgccctg gacgtatatg agaagggctt
     6781 ctctgagcag ctttaacccc gaggcccttt accgcaagac gcccctgatc gatgccattc
     6841 cgcagagcgt agactcttcg tgggccgcca tgttggacga aggcccccgg tccgggttct
     6901 gggacggaaa cgatctatgg agctatgtgc agagaacctg cgttcccgga cttcacttcg
     6961 gcggatggtt cgacttcctg ctcgacgcta ccttggttcc gtactccgag atggtccagt
     7021 ctggcgcgcc ccagaggctt gtgctcggcc cgtggagtca caacggcacg atcggcgggc
     7081 ctgagaggat atcgggagat gtgtcatacg ggtactggtc cgggcagcgt gaaggcggtc
     7141 tggatggtct gcatgaggat ccggtggaca tgtcttctcc tttcatggag gagtgcgtga
     7201 gttggctgga cttctgggtg aagggcgcag gcgattcgcc gccgaggtct tctgtgaagt
     7261 gtttcttcac ggggcatgag cctcactggg ctgagcttgg ggcgtggcct catcccaggg
     7321 cctcgggcgt gaggatggac ctcggggctg cagctggtgg tcagtctgag gccgggagtc
     7381 gaggcagggg cgtcctttct gccttctctc ctgtcttctc tgcggacggg gccttcggca
     7441 caaaccccgt ggccgccagt gagtcgtttg tgtacgatcc ggatgatcct gtttccactg
     7501 agggtggcgc ggtgtgggca tttccaaagg ccgggatggc gcctggccct tcggtgtcgt
     7561 ccgctcattt gcgggaggat gtattgacct acgattctga agtgttggga agtccgttgg
     7621 agatatgcgg cgctgcggag gttgagctgc acgcgtcgtc agacgcgccc gacacggact
     7681 tcgtcgcgat gctgctggat gtggatgaag acggtgttgc aaggtacgtt tcagacggca
     7741 tagtgagggc tagatatcgc aatagtcttg acagagagga cttcctggag cccggccaga
     7801 tctatctctt ccgcatacgc ctgaaggggt gcgcccacac tttcggccca ggccatcggg
     7861 tgcgactcac agtgacgagc tcgaacttcc cgaagtttga ccgtaatcct aatacgggag
     7921 agcctatcta tcgaagccgg cgaatgcaga aggccgtgca gacagtctac tgtgggcctg
     7981 cgcttccatc ggcggtggtg atccctgtgc tcagcggata tcaggtgcgc agtcttcatg
     8041 atgacgaccg tgagtgggcg gaaaggctgc tgaaggagag ctggggttcc gccaacatcg
     8101 tgacgcgggg acggctggtg gatgcgtcta ggcttccggg gttcgttgcg gtggcaggta
     8161 gcggcgatcg cctcggcttg ttgacgtatg aggagcgcaa cggtcagtgc gagatcgtca
     8221 ctctgaacag cttgcggcgg gggatgggca ttgggcaggg gctgatctgg agggtagtgg
     8281 agtgtgcgaa ggaggccggg gcgtctagag tgtggctgat caccactaat gacaacaccg
     8341 atgccctgag gttctaccag aaagccggtt tccgcatagt tgcggttcac caggatgctg
     8401 tggctctggc caggcgtatc aagcctgaga taccgataat gggggagaac gggataccca
     8461 ttcgcgatga gatcgaactt gaactgatgc tggatacgga tcacgggaac gtatagacgg
     8521 ggcggacagg tgaggcacgg tggcgtttcc gattgcgtgc ctcaactccc ttcgcccgcg
     8581 gaggtcggcg ccgcggggta ggcctgaggt ggttcgtgca ggcactcttc cggtacgggg
     8641 ataacggcga acccctcttg ggcgaagtgc ctatcaaggc tgagtgcagt atggatggac
     8701 tcatcccgca tcatcacaaa agatgttgca tctacgaacg agtacgattt gtcgggtcga
     8761 cggcgaagaa gatcaaaagc catgtcaagc tggtttgttg ttggatggag cacgcgaacc
     8821 tggccactta gcgcgatgtc gtcgcgaaag gaccagaagc ccgctgcggt accagtgcgt
     8881 gcgagcagca gtgtatgcaa ctcccccagg acaagactgc tggttatgaa gatgcctccc
     8941 tcgctgtggg ccagggcata ctcggcgcgc acagctgcgt ggtattggtc tgcagccatg
     9001 agcagtgcga accaagcgct cgtgtcgacg aagcagcgga tgctcatcgg tcctcccaat
     9061 ccttgcggta gatgtagcgg tcatggttta ccgccgcatc gcccggcgcg tcaggcgagt
     9121 caatcatacc gataatcctg agggcagggt cgtcctggag gggcgtctcc cgggcgatga
     9181 aactcgctat ggcctcgcga atcaactcgg ccattgcgac gttgcgacgc ctggccagta
     9241 acgacagctc atcatggacc tgaggttcga tgtagaactg catgcggtgc atgcgactac
     9301 gatcaggcac gcttgacacc tccatgatcg agcaatgtat acacatcaca ggatacatct
     9361 atacccacca tagcgtcggt agattcgcgc gtcaaggtcc ctgttccgca agtccctgtt
     9421 ccgcaagtcc ctgttctgca agggagcgcg taacgattgt tactttcggg accgcggata
     9481 ccccgcattc acacgtgcaa tctcgccaga gcaacagctg tggctcagta atataccccc
     9541 gggggtatct gcccggagat acggtcactt tttaacaata ctttcgtcga gaaacgcaat
     9601 ttttgagtac atcgatttgg gccgggggcc ccgaggggcg aatcggccgt gtcaaaggag
     9661 tcctcggggc cagataccgc cgatttattg gcgtgtcgat ggcacaacgt gccattgctt
     9721 acgcgccgct accaaatgga gccatgatat gggatattat gtcggctagc ccgcccgctt
     9781 cactgcccag acgcgccgga taccccccga ttttcgcgat ttggaggcgg tcaggcggct
     9841 cttcgaaatc gatgtactca aaagttgcgg gtatctccga aactgatggc a
//
LOCUS       DAOTAC010000062         9700 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_5998_length_9700_cov_5.589943, whole genome shotgun sequence.
ACCESSION   DAOTAC010000062 DAOTAC010000000
VERSION     DAOTAC010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 9700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9700
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5998_length_9700_cov_5.589943"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1067
                     /locus_tag="PLM74_04025"
     CDS             <1..1067
                     /locus_tag="PLM74_04025"
                     /inference="COORDINATES: protein motif:HMM:NF012892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HON42285.1"
                     /translation="FNAGITPVMITGDHKDTAVAIAHDVGIMTGAAVAVTGSELSRMP
                     DDELDRIVDRVRVYARVAPEHKVRIVDAWRRRGHVVAMTGDGVNDAPALRRADIGAAM
                     GITGTDVAKSAADMILTDDNFATIVAAVREGRVVFDNIRKSVQYLVSCNIGEIIVIFA
                     AILLGLPRPLLPIQILWLNLITDGLPALALGVDPPERDLMRRKPRGRDGSVLDRASSV
                     RLVVYGASVAGLGLMSFAMGLGWPGPINVSPDSLVAARTMCLMTMSFSQLFHAFNFRS
                     ELSSLFSVGILANPKLMLAFLTSGLLQLGIVYLPGVSPFFNVVDIHGAQFLESVGLAA
                     LMIVIGELVKAVSRMRHVRS"
     gene            1179..1724
                     /gene="raiA"
                     /locus_tag="PLM74_04030"
     CDS             1179..1724
                     /gene="raiA"
                     /locus_tag="PLM74_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HON42286.1"
                     /translation="MEITVVGRNVAVTDALRAYAEKKVTKLQRYFERGIMEAQVSMTI
                     ERGIHSVDITIQVDGLLLRGEEHTGDMYASIDGAIDNIERQIRKHKTRINRRLRQEGN
                     KLVESAVAVGPAADVVEAPEPKVVKTKRFAIKPMSVEEAIMQMELLGHDFFVFMDDSS
                     EDVNVVYRRRDGNYGLIEPEF"
     gene            1826..2962
                     /locus_tag="PLM74_04035"
     CDS             1826..2962
                     /locus_tag="PLM74_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42287.1"
                     /translation="MRPFVGFVFEGGQHSGDVEGWVARAREEMAILLAQRLVDTGLLD
                     KVVFVTDRVALADRAASFAGVDTAITSDEPAASFHFGRRLAQVVAEYDAAGFVYMSGG
                     SGILMDMAELADFVLATQARPGSIVANNVYSADMFGVADSRVALSAELPSADNGVPMM
                     VHAAGIPVYGLPPTTGNTFDIDTPSDLLVLSDVIPVLAPYAQSVRDVVAEGPVGRAVG
                     ILSAARAALARDLAEIALIGRVSPATVADLNSRTTCRLRVYSEERGMRAFGRDKPGMA
                     RSLIGKMIQACGPRAFFADLAWCSDAAFIDTRVLFAHVGAELSQEERFCSDLFLWEKV
                     PGEDVRCLVHAALDAQIPVMLGGHSLVSGAVRALAACTGRRGVV"
     gene            3019..5592
                     /gene="secA"
                     /locus_tag="PLM74_04040"
     CDS             3019..5592
                     /gene="secA"
                     /locus_tag="PLM74_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009623675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HON42288.1"
                     /translation="MFDVNAKDVRELEARLSAVSALAPEAEEATDEQLLARTSEFRNR
                     IDRGESVDSLLPEAFATAREVARRQLGLYPYDVQVMGGMVLHQGKIAEMKTGEGKTLV
                     ATMPAYLNALEGRGVHIVTVNDYLARRDAEWMGPVYRALGLSVGSILHDLDASARRQA
                     YGSDITYGTNNEFGFDYLRDNMATSADQQVQRGHNYAIVDEVDSILIDEARTPLIISG
                     QAEESTEKYFVFARIADSLKQEQDYTVDEKANSVALTESGVSRVERWLGVDNLFDEAN
                     IELTHHINAALRAKALMRRDRDYIVKEDEVIIVDEFTGRLMFGRRYSDGLHQAIEAKE
                     NVRVQRESQTLATITFQNYFRMYAKLAGMTGTAATEENEFIKIYNLHVVQVPTAKPMI
                     RQDLPDIVYKTERAKFNAVVEEICERHATGQPVLVGTVSIEKSERVSEMLTRRGVPHQ
                     VLNAKHHEREAEIIKLAGQPGTVTIATNMAGRGTDIVLGPGVPELGGLHVLGTERHES
                     RRIDNQLRGRSGRQGDPGSSRFYVSLEDDLMRLFGGEFISGMMDRLGWGEDDPIEHPQ
                     LTRAIQRAQEKVEGRNFDIRRQVLEYDDVMNKQREAIYARRDAIMRGTDLRQQAMELL
                     SVVAEGVVTAHADEKLASSEWDLAGLSAEIGSILALRAKPDLSGYAQRSWADLHDGVM
                     GMFRSAYEQKEAAVGSERLRELERAIMLYMIDSKWVDHLQAMDMLREGIGLRAYGQMD
                     PLVEYKRESFLMFNDLLKSVGEDFVRYLLRVQVRNVSESGEDEEGDRARESVGTRAPA
                     QAQAQVDGPRRPKYTVTTNREDGGSSRQPQKRAVKKVGRNDPCPCGSGKKYKKCCGAG
                     K"
     gene            5622..6738
                     /gene="prfB"
                     /locus_tag="PLM74_04045"
     CDS             join(5622..5690,5692..6738)
                     /gene="prfB"
                     /locus_tag="PLM74_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_143334208.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HON42289.1"
                     /translation="MRAELEQPIESLDARIEEMRAYLDIPGKLARLAEYEARSSEPGL
                     WDNRDAAQELMQKIATVQAQIGRFESLKSKFDDLRILAELADEEDDEHAIAECMEQID
                     ALDAAISRIELSNLLSGPYDAANAIMAIHPGAGGTESQDWAEMLMRMYSRWAERNGYR
                     VEVADLLPGEEAGIKSATLIMSGQNAFGYLKGERGVHRLVRISPFDASARRHTSFASV
                     DVIPEMGEEEIEIDPSDLKIDTYRSGGAGGQHVNKTDSAVRITHLPTGIVVQCQNERS
                     QHSNKETAMRILRGRLASLALEQQEKELAAIRGEQHEIAWGSQIRSYVFQPYTMVKDH
                     RTDIETGNVNAVMDGEIDELIEGQLRLAAQSANRGVR"
     gene            6738..7460
                     /locus_tag="PLM74_04050"
     CDS             6738..7460
                     /locus_tag="PLM74_04050"
                     /inference="COORDINATES: protein motif:HMM:NF022655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2993 domain-containing protein"
                     /protein_id="HON42290.1"
                     /translation="MAARRGKGGAARMFAIYLVLAVAITEAALPSLIEQRVERAVASG
                     FAGVGSVEARVRAFPAVRMLFGHFDRLNVNIRRAQLAGLNVDSIEVRSIGARLPLSDF
                     APGSRRWAHALGDAELVVSVLDADINDYLKTRDDALRMFSASFNEGVARLDGTVSFAG
                     LNVRVSSQGRLVIEDNARLVYVVDKLSVERSELPEFLLDALEGKIELGVDLSTLPFAT
                     VLEGIRVSAGVARVYGRTVKGG"
     gene            7465..9468
                     /locus_tag="PLM74_04055"
     CDS             7465..9468
                     /locus_tag="PLM74_04055"
                     /inference="COORDINATES: protein motif:HMM:NF039831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5693 family protein"
                     /protein_id="HON42291.1"
                     /translation="MINRRQPWTIGWLVALVVVAALTSAWIAVRRHRVEEMNRRVELC
                     MDWREGSLFAQRVGLAEADYLEQLRDAGITSIAVDEETLGSLQASGDIVMLRASELAA
                     VSSIAPELLPAAAGTAAFSGGHTVVVPLSKDAAAFIADRLPIRLQGGPSLVTVLSGRT
                     SASIIGLPLEEARNINLGPRPSALAKIAAAGLHTVLRFTNYPGVSEKWIESVVRPVDP
                     AHKVDSVIFAGTEVLGYPALTAVTARALGEEGMTYGSIEFQVQAGENQITQAAGYSVV
                     RVHSITRGEVDRGLSVQTMTDRYVRAARERNIRVMYMRPIMKQIDARALPELNDSFIR
                     AVADGLQRAGFTLGPARPFGQADPGLAPTLVVVVGIAAGCVMLLNAIWNLTFPVSAGL
                     VAVAAAGFAALSAFGRADLAKQAAALAAAIAYPSLSAFVLLSPFPTSRPTDCIVKFIV
                     ASALSLAGGLMLAGCLTSLAFMIKAKQFMGVKLMHVAPMLFLLMAYWKYVYRRGDETL
                     TASARRLLSSPILVWHAVVIGALGAAGLIYIARTGNTSVLPVGVPAWEQAMRSVLERF
                     LPVRPRTKEFLLGHPAFVLSVTLAWAGDRVVLLPLAALALIGQISLANTFAHLHTPIA
                     VTLTRVLVGAGLGLAIGLAVAGVFLAIRRGLRRSAGKIAGREG"
     gene            9476..>9700
                     /locus_tag="PLM74_04060"
     CDS             9476..>9700
                     /locus_tag="PLM74_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase CsaB"
                     /protein_id="HON42292.1"
                     /translation="MRVVVSGYHGFGNLGDEAVLAALIQQMKEIAPEAEFVALSGDPA
                     CTVSAHGIRAILRTSVSAVVRELRRADLLVS"
BASE COUNT         1943 a         2587 c         3214 g         1956 t
ORIGIN      
        1 gcttcaacgc cgggatcacg cctgtcatga tcaccggaga tcacaaagac acagctgtcg
       61 ccatagctca tgatgtagga atcatgactg gagcggctgt tgcggtcacc ggcagtgagc
      121 tgtcgaggat gcccgatgat gaactggatc ggatcgtaga ccgcgtacgc gtatatgccc
      181 gggtcgctcc tgaacacaag gtgcgtatcg tcgacgcctg gcgccgtcgc ggccacgtag
      241 tggccatgac tggggatggg gttaacgacg cgcctgcact gaggcgcgcc gatatcgggg
      301 ccgccatggg catcaccggg acggacgtag ccaagagcgc cgccgacatg attctaaccg
      361 acgacaattt cgccactatt gtagcggctg tgcgcgaggg acgggtggtc ttcgacaata
      421 tcagaaagtc agtacagtac ctcgtgtcgt gcaatattgg cgagatcatc gtcatatttg
      481 cggccatctt gttgggcctg cccaggccgc ttctgcccat ccagatcctg tggctcaacc
      541 tgatcaccga cggcctgccg gcattggccc tgggcgtaga tcctcccgag cgcgatctga
      601 tgcggaggaa gccgcggggg cgcgatggat cagtcctaga ccgagcatct tcagttcggc
      661 tggtggtcta cggggcttcc gtcgccggcc ttggcctgat gtcgtttgcg atggggcttg
      721 gatggccggg tccgatcaac gtctcgccgg atagcctcgt tgcggccagg acaatgtgct
      781 tgatgaccat gagtttctcg cagttgttcc acgcgttcaa tttccgttct gagctgagct
      841 cgctatttag cgtgggcata ctcgcgaacc ccaagttgat gctggccttc ctcacatccg
      901 gcctgcttca gctgggcatc gtgtaccttc ccggggtcag cccatttttc aacgtagtgg
      961 acatccatgg ggcccagttc ctagaatcgg tgggtctagc tgccctcatg atagttatcg
     1021 gcgaacttgt gaaggcagtg tcgcgtatgc gacacgttag gagctgaatc gatgtcttct
     1081 tgcgtttttc caagaggcgc aggattttcg ccccggaggg ggaagtacta tcctagccag
     1141 gacggcgcac tcaattagtc cggaaggggc tgatgcgaat ggagatcaca gttgtcgggc
     1201 gcaatgtcgc agtgaccgac gctcttcgcg cgtatgcgga gaagaaggtc accaaattgc
     1261 agaggtattt tgagcgcggc atcatggaag ctcaggtttc catgactatt gagcgcggca
     1321 tccacagtgt cgacatcaca atacaagtgg acggattgct tctgcgaggc gaggaacaca
     1381 ccggcgacat gtacgcgtcg atcgatgggg ctatcgataa catcgagcga cagatcagga
     1441 aacacaaaac ccgcatcaac cgcaggctgc gccaggaagg caataagctg gttgagtcgg
     1501 cggtggcggt aggccctgcg gctgatgttg tggaggcccc cgaacccaag gtcgtaaaaa
     1561 ccaagcgatt tgcgataaag ccgatgtcgg tcgaggaagc gatcatgcag atggaactgc
     1621 tcgggcacga tttctttgtg ttcatggatg attcgagcga ggatgtcaat gtagtctaca
     1681 ggcgcaggga cggcaactac ggtctgatcg agccagagtt ctgattgggt tggttagtcc
     1741 ggtacggcgg gagcggagcg cggcggcgct gccggtcccg ccgctcgtta tgacggtcgg
     1801 tggtgtgtga aggggtgtga tgcgggtgcg tccatttgtc ggtttcgtgt tcgaaggtgg
     1861 acagcattcg ggcgatgttg aaggatgggt agcccgggcg cgggaggaga tggccatcct
     1921 gctggcccag agactggtcg acacgggcct gcttgacaag gttgtgttcg taaccgaccg
     1981 tgtggccctc gcggatcgcg ccgcatcttt cgcaggggtg gatacggcaa tcacatccga
     2041 cgaacccgcc gcgtctttcc acttcgggcg gcggcttgcc caggtcgtcg ccgagtacga
     2101 cgccgccgga ttcgtctaca tgagcggcgg atccggcatt ctcatggaca tggccgaatt
     2161 ggccgacttc gttcttgcga ctcaggcccg gccgggcagt attgtagcta acaacgtcta
     2221 ctcggccgac atgttcggcg ttgccgactc aagagtggcg ctctcggcag agctcccatc
     2281 tgccgacaac ggagtgccca tgatggtgca tgcagcagga atacctgtct acggattgcc
     2341 gcccacaacc ggaaatacat tcgacatcga cacgccgtcg gatctgctag tgctaagtga
     2401 cgtaatcccg gtccttgcac cctacgccca gagtgtcagg gatgtggtgg cggaaggacc
     2461 ggtcgggaga gcggttggga ttctatcggc cgccagggcg gctttggcca gggatcttgc
     2521 tgagattgcc ctgattgggc gggtatcgcc ggccactgtg gccgatctga actcgcgcac
     2581 cacctgtcgc ctccgggtat attctgagga acgcggaatg cgcgctttcg gtcgtgacaa
     2641 gccgggaatg gccagaagcc tgattggcaa gatgattcag gcgtgcggcc ctcgggcgtt
     2701 cttcgccgat ctggcgtggt gttccgacgc cgcgttcatt gatacgcgcg tgctcttcgc
     2761 ccatgtgggc gcggagcttt cgcaggagga acggttctgt tctgacttgt ttctctggga
     2821 gaaggtgccc ggtgaggatg tcaggtgcct ggttcatgcg gcattggatg cacaaatacc
     2881 ggttatgctg ggtggacatt cgctggtgtc tggggccgtt agggcgctgg cggcgtgtac
     2941 cggcaggcgc ggtgtggtat aattgcttga tgcaaagact caagcgaggt ggctttgttg
     3001 ctcaaccttt tcaagcgtat gttcgacgtt aatgcgaagg atgtcaggga gctcgaggca
     3061 aggctttcgg ccgtatccgc gcttgcgccg gaagccgaag aggccaccga cgaacagctc
     3121 ctcgcacgta cgtctgagtt cagaaaccgc atagatcgag gcgagtccgt cgattcgtta
     3181 ctgccggagg ctttcgcgac cgcacgcgaa gtcgcgcgcc gccagctggg cctgtacccg
     3241 tacgatgtac aggtcatggg tggcatggtg ctccatcagg gcaagatagc tgagatgaag
     3301 acgggcgagg gcaagacgct cgtggctacc atgcccgcat accttaacgc cctggaaggt
     3361 cggggagtcc atatcgtcac ggtcaacgac tatctggcca ggcgcgacgc tgagtggatg
     3421 ggtcctgtgt accgtgccct cggcctcagc gtaggctcca tactgcacga ccttgacgct
     3481 tccgcgcgcc gacaggcata cggaagtgac attacgtacg gcactaacaa cgagttcggt
     3541 ttcgactatc ttcgcgacaa tatggctaca tccgccgatc agcaggtgca gaggggccat
     3601 aattacgcca tcgtagatga agtcgactct atcttgatag acgaggcgcg caccccgctg
     3661 atcatatcgg gccaggcaga ggagtccact gagaagtact ttgtgttcgc caggattgcc
     3721 gatagcctga agcaggagca ggactacacc gtagatgaga aagcgaactc agtggcccta
     3781 accgaatcag gcgtctcgcg ggtagagcga tggctgggag tcgacaacct cttcgacgag
     3841 gccaacatag aactgacgca ccatatcaac gccgcgctcc gggcgaaggc gctgatgcgc
     3901 agggacaggg attacatcgt caaagaggac gaagtaataa tcgtcgatga gttcaccggg
     3961 cgcctcatgt tcgggcggcg gtacagcgat ggcctgcacc aggcgattga ggccaaagag
     4021 aatgtaaggg tacagagaga aagccaaact ctggccacga tcacattcca gaactacttc
     4081 cgcatgtacg cgaagctggc cggcatgacg ggcacggcgg cgactgagga gaatgagttc
     4141 atcaaaatat acaacctcca tgtggtgcag gttcctacgg cgaaacccat gatcaggcag
     4201 gacctgccgg acatcgtgta caagactgaa agggccaagt tcaacgcggt cgtggaagag
     4261 atatgcgaaa ggcacgccac cggccagccg gtgctggtgg gtacagtatc gatcgaaaag
     4321 tctgagcgtg tttcggagat gcttactcgt cgaggtgtgc ctcaccaggt tctcaatgcg
     4381 aagcaccatg agcgcgaggc tgagataatc aagcttgcgg ggcagcccgg cacggttacc
     4441 atagctacga acatggccgg tcgaggcacc gacatagtgc tcggcccggg cgtgcccgag
     4501 cttgggggtt tgcacgtgtt gggtaccgaa cgccatgaga gccggagaat cgacaatcag
     4561 ctccgagggc gttcgggaag gcagggcgat cccggatcgt cgcggttcta tgtgtctctt
     4621 gaagacgact tgatgaggct gttcggcggg gagttcatat ccggcatgat ggaccggctc
     4681 ggatggggag aagacgaccc tatagaacac ccgcaactca ctcgggcgat ccagcgcgcg
     4741 caggagaagg tggaagggcg caacttcgat atcaggcggc aggtgctgga atacgacgac
     4801 gtaatgaaca agcagcgcga ggcgatatac gcgcggcgtg acgcaataat gagaggcacg
     4861 gatctgcggc aacaggcgat ggaactgctg tctgtagtgg cggaaggggt cgtgacggcg
     4921 catgcggacg agaagctcgc gtcgtcggag tgggatctcg cgggcttgtc tgcggagatc
     4981 gggagtatac tggcgttgcg tgccaagccc gacctatccg gatatgccca gcgttcgtgg
     5041 gcagatctgc acgacggggt catgggtatg ttccgaagcg cctacgagca gaaagaggcg
     5101 gcggtcggaa gcgagcgtct gcgagagctg gaacgcgcta tcatgctgta catgatcgac
     5161 tccaagtggg tcgaccactt gcaggccatg gatatgctca gggagggaat tggcctcagg
     5221 gcatatggcc agatggaccc gctcgtggag tacaagagag aatctttcct catgttcaac
     5281 gatctgctga agagcgtagg agaggatttc gttcgctacc tcctccgagt gcaggtccga
     5341 aatgtcagtg agagcggcga ggacgaggaa ggggatcgtg cccgggagtc ggtcggaacg
     5401 cgggcgccgg cgcaggcgca ggcgcaggtg gatgggccta ggcgtccaaa gtacacagtg
     5461 accaccaaca gggaggacgg aggttcgtcc aggcaaccgc agaagcgggc cgtcaagaaa
     5521 gtgggacgga atgatccatg cccgtgcggc agtgggaaga agtataagaa gtgctgtggc
     5581 gcaggcaagt agagcagagc agagcagagg gagtgtgcct gatgagagcg gaactggagc
     5641 agccgattga gtcgctggat gcgagaattg aggaaatgag ggcttatctt tgacatcccg
     5701 ggcaagctag cccgactggc agagtatgag gctcgctcga gcgagcccgg gctgtgggac
     5761 aaccgcgacg cggcccagga actgatgcag aagatcgcga cggtgcaggc ccagatcgga
     5821 agattcgagt cgctgaaatc caaattcgat gatctgcgta ttctggcgga gcttgccgat
     5881 gaagaggacg atgaacatgc gatcgccgaa tgcatggagc agatagacgc tttggatgcg
     5941 gcgatcagtc ggatagagtt gtccaatctg ctgtcagggc cttacgatgc ggccaacgca
     6001 atcatggcga ttcacccggg cgcaggcgga accgagagcc aggactgggc ggagatgctt
     6061 atgcgcatgt actcgcggtg ggcggagagg aacggctatc gtgtagaagt ggccgatctg
     6121 cttcccggtg aggaggccgg catcaagagc gctaccttga tcatgtcggg gcagaacgcg
     6181 ttcgggtatc tcaagggcga gcggggagtg cacaggcttg tgcggatatc gccgtttgac
     6241 gcatcggcgc gcaggcacac ttcatttgca tcggtggatg ttatccctga gatgggagaa
     6301 gaagagatcg agatcgaccc gtcggatctg aagatagaca catatcgttc gggcggcgcc
     6361 ggaggccagc atgttaataa gacagatagc gcggttcgca tcacgcactt gccgacaggc
     6421 atagttgtgc aatgccagaa cgagaggtcc cagcatagca acaaggagac tgccatgcgc
     6481 attctccgag gccggcttgc cagccttgcc cttgaacagc aggagaagga gttggcagcg
     6541 attcgcggag agcagcacga gatagcgtgg ggcagccaga tcagatcata tgtgttccag
     6601 ccctacacca tggtcaagga ccacagaacc gatattgaga ccggcaatgt caacgccgtg
     6661 atggacgggg agatagacga gttgattgag ggccaactca ggctggccgc tcaatcggcc
     6721 aacagaggtg tccgctgatg gcggcaagac gaggtaaggg cggcgccgcg cgcatgtttg
     6781 ccatatatct tgtgctggcc gtcgccataa ccgaggctgc gctcccgtcc ctcatcgagc
     6841 agcgcgtgga gagggcggtt gcgtccggtt ttgctggcgt gggatcagtt gaggcgcggg
     6901 tgcgggcctt tcctgccgta aggatgctct tcgggcactt cgatcggctg aacgtgaata
     6961 tccggcgagc gcagcttgcg ggcctcaacg tagactccat cgaagtccgt tccataggcg
     7021 cccgattgcc tctttcggat ttcgccccgg gctcgaggag atgggcgcac gcccttggcg
     7081 atgcggaact tgtcgtttcg gtcctcgatg ccgacatcaa tgactatctg aaaacgcgcg
     7141 acgatgcgct caggatgttc agtgcctcct tcaatgaagg tgtggccagg ctggatggta
     7201 ctgtctcctt tgcagggctc aatgtgaggg tgagttcaca gggccgtctg gtgattgagg
     7261 acaatgcacg cctcgtttat gtagtggaca agctgagcgt cgagcggagc gagttgccgg
     7321 aattcctgct tgacgcgctc gaaggcaaga tagagctggg tgtggacctg tcgacgctgc
     7381 catttgccac ggttttagag ggcatacggg ttagcgccgg cgtggcgcgc gtgtacggca
     7441 ggacagtcaa agggggctag tggcttgata aaccgcaggc agccgtggac gatcggatgg
     7501 ttggttgcac ttgtagtagt agccgcactc acatcggcct ggatagcagt ccgaaggcac
     7561 cgggtcgagg agatgaacag acgtgtcgag ctctgcatgg actggcgaga gggctctctg
     7621 ttcgcgcagc gggttgggct ggcggaggca gactaccttg agcagttgcg cgatgccggc
     7681 atcacgagca tagccgtgga cgaagagacg ctcggctcac tgcaggcatc cggtgatatc
     7741 gtgatgctca gagcctctga actggccgcg gtctcgagca tagctccgga attgcttccc
     7801 gctgccgcgg gcacggccgc attctccggc gggcacacgg tcgttgtccc gctaagcaag
     7861 gatgcggccg cgtttatcgc cgaccgacta cccatccgcc tgcagggcgg gccgtcactg
     7921 gttacggtgc tttccggccg gacgtcggct tctatcatag gccttccgct ggaagaggcg
     7981 cgaaacatca atcttggccc acgcccgtct gcgctggcca agatcgcagc cgccggtctg
     8041 cacacagtcc tgagattcac caattaccca ggcgtatccg aaaaatggat agagtcagtg
     8101 gtgcggccag tcgacccggc tcataaggtc gactcggtga tattcgccgg aactgaggtg
     8161 ctggggtatc ccgcgctaac ggctgtaacc gcgcgtgcgc tgggcgaaga gggtatgacc
     8221 tacggcagca tagagttcca ggtacaggcc ggggagaacc agatcacgca ggctgccggt
     8281 tacagtgtgg tgcgtgtgca tagcatcacc cggggtgagg tcgaccgagg gctatcagtt
     8341 cagaccatga ccgacaggta cgtgagggct gcaagggaac gcaacatacg cgttatgtat
     8401 atgcgtccaa taatgaagca gatcgacgca cgtgctttgc cggagctgaa cgattcgttc
     8461 atccgcgcag tcgcggacgg gcttcagagg gcaggcttca cacttggtcc ggcgcgcccg
     8521 tttgggcagg ccgatccggg gctcgcgccg acgctcgtgg ttgtcgttgg catagcggca
     8581 ggttgcgtaa tgcttctgaa cgccatctgg aatctgacct tccccgtttc cgcaggccta
     8641 gtggccgtgg ctgctgcggg tttcgctgcc ctgtcggctt tcggccgcgc cgatttggct
     8701 aagcaggcgg cggctctggc cgcggccata gcgtacccgt cgctgtccgc cttcgtgttg
     8761 ctgtctccgt ttcccacgtc tcggccgaca gactgtatcg tcaagttcat cgtggcgtcc
     8821 gctctttcgc ttgccggcgg cctgatgctt gcgggttgcc ttaccagcct tgcgttcatg
     8881 atcaaagcca agcagttcat gggcgtcaag ctcatgcatg tggcgccaat gttgttcctt
     8941 ctgatggcat actggaagta cgtgtaccgc cgtggcgacg agacactcac cgcatctgcc
     9001 aggcggttgc tatcgtcgcc gattctcgta tggcatgcgg ttgtcatagg agctctggga
     9061 gcggccggct taatctacat tgcgcgcaca ggcaacacat cggtattgcc tgtgggcgtg
     9121 cctgcgtggg agcaggcgat gcgctcggtg ctggaaaggt ttctgcctgt gaggccgagg
     9181 acaaaggaat tcctgctagg ccatcccgcc ttcgtgcttt cggtgaccct ggcctgggca
     9241 ggcgatcgtg ttgtgttgct gcccttggcc gcgctggccc tgataggtca gatttcgctg
     9301 gcgaatacct ttgctcatct gcacacgccc atcgcggtca ccctcaccag ggtgctggtg
     9361 ggcgcgggac tggggctcgc catcgggctc gcagtcgccg gcgtgttcct ggcaatccgt
     9421 cgtggcctgc ggcgatcggc aggcaagatt gccgggcggg aaggatgacg ccggcatgcg
     9481 tgttgtggtt tcggggtatc acgggttcgg caacctgggc gatgaggcgg tattggctgc
     9541 cctcattcag cagatgaagg agattgcgcc cgaggcagag tttgtggctc tctcgggcga
     9601 ccctgcgtgt accgtatcgg cccacggaat ccgcgcgatt ttgcgcacct ctgtttctgc
     9661 cgtcgtgcga gaactccggc gggcggatct cctagtgagc
//
LOCUS       DAOTAC010000063         9618 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_6059_length_9618_cov_4.805814, whole genome shotgun sequence.
ACCESSION   DAOTAC010000063 DAOTAC010000000
VERSION     DAOTAC010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 9618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9618
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6059_length_9618_cov_4.805814"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /locus_tag="PLM74_04065"
     CDS             <1..565
                     /locus_tag="PLM74_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42293.1"
                     /translation="CERSWFVSIAEQGEVVSPDGSLAAWESMAEGRWTLHRTDWQLGG
                     PGGEREWELVGHRGPIHSVSWKGDSSQLAGATDGGVVIWNATSGKLIRSMPVGGPGVK
                     GQGAAGPGATGPGATYVSWSPGPGGRWILAGLDSGTSVVIDADTGERLAEVPTRHRGS
                     SAWAPKGDRLATVGIDNAVHLYGERGK"
     gene            complement(673..1620)
                     /locus_tag="PLM74_04070"
     CDS             complement(673..1620)
                     /locus_tag="PLM74_04070"
                     /inference="COORDINATES: protein motif:HMM:NF016114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HON42294.1"
                     /translation="MSDDKGRTEHDLGSLVLISEMYYIQGKTQKDIADSLGLSRPAVS
                     RILQAARDRGIVSISIVDPSEQLKTLEEELTRAFGLKGCRLSPSGEDYGILKARLGYR
                     AAELLLTGLEPGDVIGIGASSTVYEAVKAFRETRSYPGLRVVPLSGGSLGIDGQPQIN
                     QVVHMAAQRLNAESIYLNAPLYVGKDSTARLLMQDTYIRQCTSLWDQLGNALIGVGRG
                     STVDAQDPYFIAALEAVRQSRVAAAVCGWFVDGEGNPIRGRNTTSVSVDQLRKARNAI
                     AVAGGKSKARAVHSVLRGKFVTHLVTDEDVAREILFMES"
     gene            1838..2896
                     /locus_tag="PLM74_04075"
     CDS             1838..2896
                     /locus_tag="PLM74_04075"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HON42295.1"
                     /translation="MFVKSKAAVITGKGQVEVREIDMPEPGQGEVLVRQKAVALCTLE
                     QRFFTGVFPRYPGCWGHEVAGIVEAIGPNTCTPLKVGDYVARGGGSACGECYECAMGR
                     DSFCPVEKERLHKQQNSSRADGISGIFGMSQYASVDPHNLVKMSKSVPFEEAALAEPI
                     ACAVNSADKLDIELGQTVVVIGAGAMGLANVLVARARGAFVVVSDLDPARRAHALKAG
                     AHAAIDPNSGDIAGQIEAINDGRRADVVIVAIGVKPANDDALSLLEPNGKMMLFASAH
                     PAVPMSIDPNIVHRSGIWITGSTGKDRRALYQASLLISRGIINVKPMIEKVIPLDQAQ
                     RAFEEAVKVDTYRVVITM"
     gene            2953..3804
                     /locus_tag="PLM74_04080"
     CDS             2953..3804
                     /locus_tag="PLM74_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HON42296.1"
                     /translation="MLMAMSPILQDARERQYGVAAPGVNDMQTVSAVFEAARELRAPM
                     ILDCVEMNDLEAVADITKFFAGRYPEVPAALNLDHGHSFDVCARAIRAGFTSVMIDCS
                     KLPFDENVRITAEVVRMAHAAGVSVEAELGRVGSARNYSEDRTAPLTDPAEAVEFVKR
                     TSVDCLTVAIGTAHGLYEGETPELDFQRLAELRAAVPVPLVLHGGSSTGDDKLARAVR
                     AGISKINLFSDLSSAGTNAIRALLASGRTTALGFLPLSMVYGAGIEAYKQMVMHYIEV
                     FGSRGVL"
     gene            4011..4895
                     /locus_tag="PLM74_04085"
     CDS             4011..4895
                     /locus_tag="PLM74_04085"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HON42297.1"
                     /translation="MNTCSARLHSEERKTALIALLPSLVIFALFTVYPIIYSLYLSLF
                     DCSLLGGPRTFVGLGNYAKMLASADFRVSMANTVKYAVGTVPIGAALSMFVAVLLNQK
                     IRGRGFYRVAYFMPVVTSMVAVAIVWTWMFDPYYGLFNALLERIGINGPAWLADPRWS
                     LPAVIILGIWKNLGYNMVIFLAGLQDVPVEYYESAEIDGAGGWQKFRYVTMPLMRGSI
                     GFVLIMSTIKSFQVFGQVYVMTGGGPMNSTMVLVYYIYRQAFEFYRSGYASAVAWFLF
                     AVILVLTMLQKRFLKVEN"
     gene            4919..5809
                     /locus_tag="PLM74_04090"
     CDS             4919..5809
                     /locus_tag="PLM74_04090"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HON42298.1"
                     /translation="MSTIVSMTNSGATDAVRAKRRRVVTKTAGLGLVHIVLILGAIFM
                     VLPFVWMLLTSFKDRSEVFTYSIQWLPSRINLDGYREVWSEIPFARMYLNSFIVAISV
                     TLCQLVTSALAGYAFARLDFPGRDVVFMIYLSAMMIPHQATVIPTFLLVRKFNMVNRY
                     SSLILPFAAGPFGAFLLRQFFMGMPRSLEEAAIIDGCSRFGVLRSVVLPLCKPALVTL
                     GLFQFMFSWNDFMWPLIVTNKMSMRTLQVGLALLRAEVSTEWPMLMAATVMATVPILV
                     AFLAAQRQFIESIAFTGLKY"
     gene            5937..7232
                     /locus_tag="PLM74_04095"
     CDS             5937..7232
                     /locus_tag="PLM74_04095"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HON42299.1"
                     /translation="MRRCCGKRMVISCLAAVTLLALCVGSMAAAPAKVKLTYWHFLGG
                     DMGKRHETLIKEFNKANPDIEVESLYSGNAWTMRDKLLAAVAGKQPPDVSMIDQFWAA
                     QLASTGAIIKMQTLIDGPDGIDKADVNKTAWMTATVDNEIWTMPYAMSNIVLYYNKDM
                     FKAVGLDPNKPPTTWGELVDYAKKLTRDVNGDGKIDEWGLSFPIQAGTGTVYYYITFL
                     WQAGGELYNADYSKVAFNSPAGVEALQFWMDLVYKHNVLPLAPPTEGFTVGRIAMELA
                     SSSTLETRQAKCKFPIGVAHIPSGKHKVTGVGGNNLAIFKNTSAKEAAAWKFVKWMSS
                     PEMNLRWSTMTGYTPLRDSVVNSQGYKDYLKANPEVATMAGQMSYARPRPNNETYPEV
                     SRILGLAVEKALFSKADPKQLLDEAAVESNEYIKSITGK"
     gene            7355..8152
                     /locus_tag="PLM74_04100"
     CDS             7355..8152
                     /locus_tag="PLM74_04100"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HON42300.1"
                     /translation="MYFGTTTMVFYSKPLDEAIAAISRCGFDYAELWMDQVWREAPER
                     AESSVRAMLDKHGMKATVHCPIMDINIASPNAGIRKESVKQLLDCIDFAGQIDARLVV
                     VHPGRRFSLKESVEEHWAAQVEALGCAFDRAKRLGVTLALENMEIDKEIASVRDYPDM
                     LRVIDDCGVPDLKITLDTAHMRDTQVILRFIDKLGPRIAHVHVSDATDKALHLRIGEG
                     SLDLPAIVRALEDHRFAGVYSLETYIPGDEEALAEERRKLGNLFAGA"
     gene            8346..9221
                     /locus_tag="PLM74_04105"
     CDS             8346..9221
                     /locus_tag="PLM74_04105"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HON42301.1"
                     /translation="MGYPVVPGHEASGVVVQAGEAVRRPLAPGTRVSLDLLNRCGECE
                     FCRTGRGNLCKYMYRGKLNVLGGFGQYVSVPSEQVFEIGESISFEEAALAEPTACVIH
                     SLGKANARFAETMLVYGVGPMGMLHVIVGKAMGLEVGVVDPDPTRREMALSLGARFAA
                     ASGPDEELKSVVVDGFGASPDIVIVTAPAVQAAESACALAGNTSRIVLFGMFPKGAMM
                     DADPNKIHYSELQIMGSESRTPLDFHTAVCAITRGIVDVKPMVSQVVPLDRVEQALSE
                     APSVDTMRVVLSLND"
     gene            9250..>9618
                     /locus_tag="PLM74_04110"
     CDS             9250..>9618
                     /locus_tag="PLM74_04110"
                     /inference="COORDINATES: protein motif:HMM:NF013916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42302.1"
                     /translation="MIGKHIRMKSLINPATGRTVIVAMDHGQIIGPAQGLESPLDAFR
                     RVVRGRPDAILTTRGMVEHGFEALDSGVGLIIRITGGFTVLGGRFEEAMISSATHALK
                     LGATGVAVTVKFGHEREDEFT"
BASE COUNT         1997 a         2565 c         3046 g         2010 t
ORIGIN      
        1 gtgcgagcgc agctggttcg tgagcatcgc cgagcagggc gaggtcgtga gcccagacgg
       61 tagtctggcg gcctgggagt cgatggcgga aggacgctgg acgcttcacc gcaccgactg
      121 gcaactaggc ggtccgggcg gagagcgcga gtgggaactc gtggggcacc gcgggccgat
      181 ccacagtgtg tcatggaagg gcgacagttc ccaactggcg ggcgcgactg atggcggtgt
      241 tgtgatctgg aatgcgacga gcggcaagtt gattcggtcc atgcccgtcg gagggccggg
      301 agtgaagggg caaggagcgg cggggcccgg agccaccggg ccgggagcga catatgtctc
      361 gtggagcccg gggccaggtg ggcgctggat attagcaggc cttgatagtg gaacctccgt
      421 tgtgatcgat gcggacaccg gtgagcgcct ggccgaggtg ccgacgcgcc accggggctc
      481 gtcggcctgg gccccaaaag gcgatcgttt ggcgacggta ggcatcgaca acgccgtgca
      541 cctgtacggt gagcggggca agtgagacgg ggagtttgca agcgggcgaa ccgctgatcg
      601 agcgggcacc tgagccgacg agcacctgag cggacgggcc cctgagccga cgagcacctg
      661 agccgacgag ctctaggact ccatgaacag gatttctcgg gcgacgtctt catcggttac
      721 gagatgtgtc acgaacttgc ctcgcaggac tgaatgaacc gctcgcgcct tgctctttcc
      781 gcctgcaacg gcgattgcat tgcgcgcctt tctcagctga tcgacgctca cagacgtagt
      841 gttcctgcct ctgatcggat ttccctcgcc gtcaacgaac cacccgcaca ctgcagccgc
      901 cactcgggac tgcctgactg cctccagggc ggcgatgaag tagggatcct gggcgtcgac
      961 ggtgctccct ctgcctacgc cgatcagagc atttccgagc tggtcccaaa ggctggtgca
     1021 ctgcctgatg taagtgtcct gcatcaagag ccgggcggtg ctgtctttgc ccacatacag
     1081 cggagcattg aggtatatgg actctgcatt caggcgctga gcagccatgt gtacgacctg
     1141 gttgatctgc ggttggccgt cgatgccaag gctcccgccc gacagcggaa ccaccctgag
     1201 gcccgggtag ctccgtgttt cccgaaaggc cttgacggcc tcgtacacgg tggagctggc
     1261 cccaattcct attacgtcgc ccggttccag gcccgtcaga agcaactccg ccgctctgta
     1321 gcccagacgc gccttgagaa tgccgtaatc ctcacccgac ggagagagcc tgcaaccttt
     1381 gagcccgaag gcgcgagtca gttcttcttc gagcgttttc agctgttccg atgggtcgac
     1441 tatggatatg ctcactattc cccggtctct cgcggcttgc agaatccttg acacagccgg
     1501 tcgcgatagc ccgaggctgt ccgcgatgtc cttctgcgtc ttcccctgaa tgtagtacat
     1561 ctcgcttatc agcaccagag aaccgagatc atgttcagtc ctacctttgt cgtctgacac
     1621 ccgggcccct cccacagcat gttcatcccg ctcctgccgc agctggcggg ttgattattt
     1681 gacacgacca gggagatttc cttcctccgg ccagtgaggc ctcgatgcgc tgaacaaatg
     1741 acagaaagga attccacacc ccgtatagaa gatggactgg cgagtcgaca gatattgctg
     1801 cgaggattat cgtcttgcgg tatggggagg tatggccatg tttgtgaagt cgaaggcggc
     1861 ggtgataacc ggaaaaggac aggtggaggt gcgggaaata gatatgcctg agccggggca
     1921 gggtgaggtg cttgtgaggc agaaagccgt ggctctatgc actttggagc agaggttctt
     1981 cacaggggtt ttccctcgct atccagggtg ctggggtcat gaggtggcgg gtatcgtgga
     2041 ggcgatagga ccgaatacct gcactccgct gaaggttggc gactatgtag cccgcggcgg
     2101 gggcagcgcc tgcggcgaat gttatgagtg tgccatgggt cgagactcgt tctgcccggt
     2161 cgagaaggag cgcctacata aacagcagaa tagtagcagg gcggacggga tttccggcat
     2221 cttcggcatg tcgcagtatg cttcggtcga tccgcacaac ctggtcaaga tgtcaaaaag
     2281 cgtgcccttt gaggaggcgg ccttggccga accgattgcc tgtgccgtga atagtgcaga
     2341 caagctcgac atagaactgg ggcagacagt tgtggtaata ggtgcgggcg ctatggggct
     2401 ggccaatgtc ttggttgcac gggcgagagg tgcgtttgtg gttgtgagcg acctggatcc
     2461 tgcaaggcgc gcgcacgctc tgaaggccgg ggcgcatgcc gctatcgacc ccaacagcgg
     2521 tgacattgcc ggccagattg aggcgatcaa cgatggccgt agagccgatg tggtaatagt
     2581 ggcgatagga gtcaagcctg ctaatgacga tgccctgagt cttctggagc ccaacggcaa
     2641 gatgatgctg ttcgcgtcgg ctcatccggc ggtgcccatg tccatcgatc ccaatatagt
     2701 acatagatcc gggatatgga tcacaggatc caccggcaag gatcgaagag ctctctatca
     2761 ggcctcatta ctcatttctc gtggaatcat caatgtcaag ccgatgattg agaaggtcat
     2821 ccctcttgac caggcgcaac gggctttcga agaagcggtt aaggtggata cctatcgcgt
     2881 tgtaatcacg atgtagccac tgcgggcagg actgcccatc atgaactagc ctagatgagg
     2941 aggaatagcc tgatgctcat ggcaatgagt ccaattctgc aggatgctcg agaaagacaa
     3001 tacggtgtgg cggcgcctgg ggtcaacgat atgcagacgg taagcgctgt gtttgaggcc
     3061 gcgcgcgagc ttcgtgcgcc gatgatcctg gactgcgttg agatgaacga tcttgaggca
     3121 gtggctgaca taaccaagtt cttcgcaggt agatacccgg aggtgcccgc tgctctcaat
     3181 ctcgatcacg ggcatagctt cgatgtgtgc gccagggcga tcagagccgg gttcacttcg
     3241 gtgatgatcg actgctccaa attgcccttc gatgagaacg tgaggatcac ggcagaggta
     3301 gtgaggatgg ctcacgcagc aggagtatcg gtcgaagctg aactcggtag ggtaggcagc
     3361 gcccgcaact actccgagga ccgaactgct cccctcactg atcccgcgga ggcagtggaa
     3421 ttcgtcaagc ggacctcagt ggattgcctg accgtggcta ttgggacggc acacgggctg
     3481 tacgaaggcg aaacgcctga gctagatttc cagcgactgg cggagcttcg cgcagctgtg
     3541 ccggtgccct tggtgcttca cggcggttcg tctaccggcg acgataagct ggcccgcgcg
     3601 gtgagggcag gcatcagcaa gatcaacctg ttcagcgacc tatcgtcggc aggcacgaat
     3661 gccatccggg cgcttttggc cagcggcagg actaccgctc ttggattcct tccacttagc
     3721 atggtgtatg gagcgggcat cgaagcctac aagcagatgg taatgcacta catcgaagtg
     3781 ttcgggtctc gcggtgttct gtagatctca atgaatatca agaaaacata gtgcctgcgc
     3841 aaaaggagtg tcgaaagaaa ctttcatatt gcagaaggaa tttccgaagc ctgcggcgaa
     3901 cagatgtctc gaactcatct cacgggctct gatcgacgac gtgtggactc aggccgacaa
     3961 cgactgaggc tgtcggcgca aaagatgtgt tgtgggggtt ggccacgtcg atgaatactt
     4021 gcagcgctcg cttgcactct gaggaaagga agacggcgct catcgctctg ttgccgtcgc
     4081 tcgtgatctt cgctctcttc acagtctacc caatcatcta ctcactctat ctgagccttt
     4141 tcgactgctc gctgctggga ggcccccgaa cgtttgtggg gctcggcaac tacgccaaga
     4201 tgcttgccag cgccgacttc agggtgtcta tggccaatac cgtgaaatac gctgtgggca
     4261 cagttcccat cggggcggct ctgtcgatgt tcgtggcggt ccttctcaat cagaagatca
     4321 gaggccgcgg cttctacaga gtagcctact tcatgccggt agtcacgtcc atggtggctg
     4381 tggccatagt gtggacgtgg atgttcgatc catactacgg tcttttcaat gcgctcctcg
     4441 agcgaatcgg cattaacggg ccggcgtggc ttgccgaccc tcgctggtcg ctgcctgcag
     4501 taatcatcct gggcatatgg aagaacctcg ggtacaacat ggtgatcttc ctggccggct
     4561 tgcaggacgt gccggtcgag tactacgaga gtgcggagat cgacggcgcc ggcgggtggc
     4621 agaagttccg ctatgtgact atgccgttga tgcgtggttc gatcgggttc gtgctcatca
     4681 tgagcacgat caaatcgttc caggtgttcg gacaggtgta cgtcatgaca ggcggcgggc
     4741 cgatgaactc cacaatggtc ttggtctact acatatacag gcaggcgttt gagttctaca
     4801 ggagcggata tgcctcggca gttgcttggt tcctgtttgc cgtgatactt gttctcacta
     4861 tgctccagaa aagattcctc aaagtggaga actgacgaga gggcgaggtg cagcagcgat
     4921 gtctacaatc gtgagcatga caaacagcgg tgccaccgac gccgtccgcg ccaagcgcag
     4981 gcgtgttgtg acgaaaaccg cgggccttgg gctcgtgcac atagtcctaa tcctgggcgc
     5041 catattcatg gtgctgccct ttgtgtggat gttgcttacg tctttcaagg atagaagcga
     5101 ggtgttcacg tactccatcc agtggctgcc gtctcggatt aacctcgacg gctaccggga
     5161 ggtgtggtcg gagatacctt tcgcgcgcat gtacctgaac agcttcattg tcgctatatc
     5221 ggtcaccctg tgccagctgg ttacgagcgc cctggcgggg tacgccttcg cccgactcga
     5281 cttcccgggc agggacgtgg tgttcatgat atatctttcg gccatgatga taccgcacca
     5341 agctaccgtc atccccacat tcctactggt gcgaaagttc aacatggtaa acaggtattc
     5401 ttcgctcata ctgccctttg ccgccggacc gttcggcgca tttctgctgc ggcagttctt
     5461 catgggcatg ccgagatctc tggaggaggc ggccatcatc gatggctgtt ctcggttcgg
     5521 tgttcttcgt agtgtagtat tgcctctgtg caaacctgcg ttggtcactc ttgggctgtt
     5581 tcagtttatg ttctcctgga acgatttcat gtggccgctg atcgtaacga ataagatgtc
     5641 tatgaggacg ctccaagtcg gcttggccct gctgcgtgcg gaggtctcga ccgaatggcc
     5701 catgctcatg gctgctacag ttatggcaac agttccgata ttagtggcct tcctggctgc
     5761 acagaggcaa tttatcgaga gtatcgcctt tacaggcctc aagtactgag gatgatgcat
     5821 tcgcgtacgg aaggagggca gttaaggctg gactcggatg gctgtaagac gcggacctgg
     5881 gcccccacgg cgcccatggc gccaggggca ttaatctacg aagggagttt gactcaatga
     5941 gacggtgctg tgggaaacgg atggtcatct cttgcctggc tgctgtgacg cttctggcac
     6001 tctgtgttgg ctccatggcg gcggcgcccg ccaaggtcaa actgacctac tggcattttc
     6061 tcggcggtga tatggggaag cgccatgaga ccctaatcaa ggagttcaac aaggctaacc
     6121 ccgacatcga ggtggagtcg ctctactcgg gcaacgcctg gaccatgcgc gacaaactgc
     6181 tggccgcagt ggccgggaag caacccccgg atgtgtcgat gatagaccag ttctgggcag
     6241 cgcagctggc gtcgaccggc gcgatcataa agatgcagac gctgatagat gggcccgatg
     6301 ggatcgacaa ggctgatgtg aacaagactg cctggatgac ggcgaccgtg gataacgaga
     6361 tatggactat gccgtacgcc atgagcaaca tagtgctgta ctacaacaag gacatgttca
     6421 aggctgttgg gctcgacccg aacaagcccc cgaccacatg gggagaactg gtcgactacg
     6481 ctaagaagct gacgcgggat gtgaacggcg acggcaagat cgacgagtgg ggcctgtcat
     6541 tcccgattca ggccggcacc gggaccgtct actattacat cacgttcctg tggcaggcag
     6601 gcggagagct ctacaatgcc gactatagca aagtggcgtt caacagcccc gccggcgtag
     6661 aagcacttca gttctggatg gaccttgtat ataagcataa tgtattgcct ctggcgcctc
     6721 ctaccgaagg attcacagtg ggcaggatcg caatggagct tgccagctcg tccacgctcg
     6781 agaccaggca ggccaagtgc aagttcccca tcggcgtggc gcatatcccg tcgggcaagc
     6841 acaaggtcac aggagtcggc ggcaacaacc tggctatatt caagaacacc tctgctaagg
     6901 aggctgccgc ctggaagttc gtgaagtgga tgagcagccc cgagatgaac ctcaggtgga
     6961 gcaccatgac cggttataca ccactccgcg actctgtggt taactcgcaa ggctataagg
     7021 actacctcaa ggccaaccct gaggtggcca cgatggcagg gcagatgtct tatgccaggc
     7081 cccgcccgaa caacgagacg taccccgagg tatcgcgcat tcttgggctg gcagtggaga
     7141 aggccctgtt ctcgaaggcc gatcccaagc agcttctgga cgaggcggca gtggaatcca
     7201 acgagtacat aaagtctatc acgggcaagt agaacaaact ttgtaaagac agtgacaggt
     7261 actggctgat tggacaacgt ggttccgggc gcgcccggga gaaccgggac tggcgcccgg
     7321 aaccatcaat cggaattgtg gaagtgagaa atgcatgtac ttcggcacaa caacgatggt
     7381 tttctactcg aagccccttg atgaggcgat tgcagccata tccagatgcg gatttgacta
     7441 cgcggagctg tggatggacc aagtatggcg cgaggcgccg gagcgcgctg aaagcagtgt
     7501 ccgcgcaatg ctcgacaagc acgggatgaa ggctacagtc cattgcccga tcatggacat
     7561 caacattgcg tctcccaacg ccggaatcag gaaggaatcc gtgaagcagc tgctcgattg
     7621 tatagatttc gctggtcaga tcgacgcacg tctcgtggta gtccaccccg gcaggcggtt
     7681 ttctctcaag gagtcagtcg aggagcactg ggcagcgcag gtcgaggcac tcggctgtgc
     7741 gttcgacaga gcaaagcgtc tgggcgtgac tctggctctg gagaacatgg agatagacaa
     7801 ggaaatagcg tcggttaggg attaccccga catgctcaga gtgattgatg actgcggtgt
     7861 gccggacctc aagattacgc tggataccgc gcacatgcgc gatacgcagg tcatattgcg
     7921 gttcattgac aagcttggcc cgaggatcgc tcacgtgcac gtgagcgacg ccacagataa
     7981 ggccctccac ctccgaatag gcgaggggtc gttggacttg ccggccatag tgcgagctct
     8041 cgaagatcac aggttcgcgg gtgtgtattc acttgagacg tacataccgg gagacgaaga
     8101 ggctctggcg gaggagcggc ggaagctggg taacctgttt gccggagcgt gaatcggagg
     8161 atcgtgcgga gggaggatgg tttagatggc gactatgaag gcagcggtcc tagttgggcc
     8221 acgcagtatc gagatacagg agagggagat accgtctgtc gggcccaatg aggtgctcgt
     8281 caatgtgaag gcctgcggga tatgtagttt cgagagacgt ctcttcactg gagagaaaga
     8341 agtgcatagg ctacccggta gttccggggc acgaggcgtc tggcgtggtt gtgcaggcgg
     8401 gagaggctgt gcggcgaccg cttgcgcccg gaacccgtgt gtcgctggac cttctgaaca
     8461 ggtgcggcga gtgtgagttc tgccgtaccg gcaggggcaa tctgtgtaag tacatgtacc
     8521 gcggcaagct gaacgtcctg ggcggtttcg ggcagtacgt atcggtgccg agcgagcagg
     8581 tattcgagat cggtgagtcg atcagtttcg aggaggcggc cttggctgag ccgactgcgt
     8641 gcgtcatcca ttcgctgggc aaggcgaatg cgcggttcgc cgagaccatg ctggtgtatg
     8701 gtgtcggccc catggggatg ctccatgtca tcgtaggaaa agccatgggt ctcgaagtgg
     8761 gcgtggtaga tcccgatcct acccgtagag agatggcgct ttcgctgggc gcaaggttcg
     8821 cggcggcgtc cggacccgat gaggagctca agtctgtcgt agtcgacggt ttcggggctt
     8881 cccctgacat cgttatcgtc actgctccgg ctgtgcaggc ggctgagtcg gcgtgcgccc
     8941 tagcggggaa tacctccagg atagtgctgt tcggcatgtt ccccaaaggc gcaatgatgg
     9001 atgcggatcc caacaagata cactactcag aattgcagat aatgggctcg gaaagccgta
     9061 cgcccctgga ctttcacacc gcggtctgcg ccataacccg aggcattgtc gatgtgaagc
     9121 ccatggtctc gcaagtcgtg ccgctcgacc gggtcgagca ggcgctttca gaggcgcctt
     9181 cggtcgatac gatgcgggtc gtgctttcgc tcaatgactg atacttgata agcggaaggt
     9241 gtggtagtct tgataggcaa gcatattaga atgaaatcgc tcatcaaccc ggcgaccgga
     9301 cgcaccgtaa ttgtggcaat ggaccatggg cagataatcg ggcctgcaca gggcctggaa
     9361 agcccgcttg atgcattccg gcgtgtcgtt cgcggcaggc cggacgcgat tttgaccacc
     9421 cgcggcatgg tagagcacgg cttcgaggcg ctagactcgg gcgtgggcct cataatccgg
     9481 atcaccggag gcttcacagt cctcggaggc aggttcgaag aggccatgat cagctcagct
     9541 acgcatgcgc tcaagctcgg cgccacggga gtggccgtga ctgtcaagtt cggccatgag
     9601 cgtgaagatg agttcacg
//
LOCUS       DAOTAC010000064         9608 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_6069_length_9608_cov_4.521407, whole genome shotgun sequence.
ACCESSION   DAOTAC010000064 DAOTAC010000000
VERSION     DAOTAC010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 9608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9608
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6069_length_9608_cov_4.521407"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1243
                     /locus_tag="PLM74_04115"
     CDS             <1..1243
                     /locus_tag="PLM74_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019123308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HON42303.1"
                     /translation="VEHDEETIRSADYVIDIGPGAGAHGGEVIAAGTVGEVQRNPASL
                     TGQYLSGARTIPVPPARRSPNGKSLVIKGAREHNLKSLDVSIPLGLFVCVTGVSGSGK
                     STLINEILHARLAHDLNRAHARPGAHDDILGIEYLDKVIGIDQSPIGRTPRSNPATYT
                     GVFDLIREVFARTTEARAKGYKPGRFSFNVKGGRCEACRGDGIIKIEMHFLPDVYVPC
                     EVCHGARYNRETLDVKYKSKSIADVLAMKVEEALEFFYNVPRIRRKLQTLWDVGLGYI
                     QLGQPATTLSGGEAQRVKLATELSRRTNGKTLYILDEPTTGLHFADVGKLLEVLQKLV
                     DGGDTVLVIEHNLDVIKTADHIIDLGPEGGDRGGRLIAEGTPEQVAAIRESYTGQFLA
                     GILRTEAKSEHGLEAMTGAVG"
     gene            1230..3080
                     /gene="uvrC"
                     /locus_tag="PLM74_04120"
     CDS             1230..3080
                     /gene="uvrC"
                     /locus_tag="PLM74_04120"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015759320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HON42304.1"
                     /translation="MPLDDTLAGLPTSPGVYLMKDDAGQVIYVGKAINLRNRVRSYFQ
                     SSRNHSPKVSRMVERIADIDYLVTGSELEALILECNLIKKHRPHYNVRLRDDKTYPFI
                     RVTVNEPYPRIFMTRTVVRDGSRYFGPYTDVPAVRETLRMLKTLFPLRQCSRKFDPES
                     PARRPCLNFHIRRCLGPCSGQVEPRLYREMVDEVVLFLEGRQADLIESLRRRMEAAAD
                     GLDFEQAARLRDQMRAVEKVVEKQRIVSDAGVDQDIIAFATDPWGACVRVMFVRDGKI
                     VGSQHFMLGGADGADDAEMLSAFIKEYYSEDSPVPRELLLQAAIDDADILEKWLAGRR
                     GGAVSVKAPQRGARRKLVELAAQNAREYLTRARAAEQSEMRTRMDGLAELAAYLKLGA
                     PPARIECYDISNLQGTDAVGSMIVFEEGRPAKSQYRRYRIRHVDGQDDFAMMGEVIRR
                     RLARATGENPDPRFGRLPDLIVVDGGKGQLNSAGAAVDAMAQSAGLPRVPVVSLAERN
                     EEIFLRGESAPLMLPRESKSLRLMQHMRDEAHRFAVAYHRALRGKKGIASELDQVPGI
                     GKKRLVALMKRFGSLEALASASEDELVSVHGMTAPAARAVWEHLHPQEGT"
     gene            3081..3779
                     /locus_tag="PLM74_04125"
     CDS             3081..3779
                     /locus_tag="PLM74_04125"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HON42305.1"
                     /translation="MNARISGGAAKPAAVTATVTATVDSRIGQVRAEAGVGGLRRVLL
                     PQVDTSRLLHLPLPGPIIRTLAGGALTPATLLSNLGLNQAMIGPKELAAGPNRISRSG
                     SRWAGADPRDVLTSVLHDLKLFFDGDLDPRGLLFDYPIDLTWCSHFTQQIMTGMASIP
                     PGTVTTYGALATAAQSPRAARAAGSVVGANPLGVVVPCHRVIGSNGALTGFGGGLPLK
                     VALLALEETAVTQS"
     gene            3858..4244
                     /locus_tag="PLM74_04130"
     CDS             3858..4244
                     /locus_tag="PLM74_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HON42306.1"
                     /translation="MKRRELLGKCPVCGEGLDVTRLACRSCGTAIEGRFDTCKFCRLT
                     PEHRAFVDVFIKNRGNIKEVEKELGISYPTVRNRLDAVIAALGYRVDEPAMEKGDDGA
                     HRKAIVEALARGEISADDAVKQLRVK"
     gene            4263..5414
                     /locus_tag="PLM74_04135"
     CDS             4263..5414
                     /locus_tag="PLM74_04135"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HON42307.1"
                     /translation="MSEERKIILNLLAEGRITADEAEQLLEALDESDLYGMGDSGTVD
                     EVDQVGPAGQVHQADQGELGSRLEARVDVIPDDLSERLSKAVSEMAEAGQELPDRLAR
                     MLGSMFGSFGWAFGPVGVQAEQTFESEMPADCVISVIDFATSNGSVKVTGWDRSGYKV
                     VARAMVRGAETKSEAERRLASALSLSLEGQVMRLECTDGTLSNSLSIEAYVPRANTYD
                     VKVSSRNGSAKLELLTAGSVDVRSANGRVVLEDITAAKVTASARNGSVTASGDLGDAS
                     LETANGSVSVVLSYRSAGRLRMNTGNGSVRLNVPRDAGVIYEISAHTVNGSVRTSLEG
                     AEVRRERGSGRDAGRTMSVHTRPGSAVPLPPVVAVEARALNGSVSVHSV"
     gene            5442..5810
                     /locus_tag="PLM74_04140"
     CDS             5442..5810
                     /locus_tag="PLM74_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42308.1"
                     /translation="MEAEKRQILEMVRDGKLTVEEAQKIMDAMEQGDEGMPPATATRP
                     PRFLRVRVKDEDGSKVNVSVPISLVRVLWKFIPRDAMRELEGHNIDIDAILAAVNEGA
                     QGNLVDVEDEDGTKVEIIVE"
     gene            5899..6093
                     /locus_tag="PLM74_04145"
     CDS             5899..6093
                     /locus_tag="PLM74_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HON42309.1"
                     /translation="MQRRLYRSVRERMLGGVCGGIADYLGTDPTLIRLVWVALSLFTR
                     GFPGVILYIVGWIVIPEEPV"
     gene            6121..6522
                     /locus_tag="PLM74_04150"
     CDS             6121..6522
                     /locus_tag="PLM74_04150"
                     /inference="COORDINATES: protein motif:HMM:NF015953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HON42310.1"
                     /translation="MAGNYNDRPHGTMPGWLVKWLINALALLIVSYVPYGRSQLIHID
                     GAGAALVAAMVLGIVNTFIRPIFIFLSLPMQFATFGLFTLVINALMLMLGDWLMGSSL
                     DLPGGLFAAIIASVLLGLTGAALNAIIRGDK"
     gene            6526..7320
                     /locus_tag="PLM74_04155"
     CDS             6526..7320
                     /locus_tag="PLM74_04155"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HON42311.1"
                     /translation="MIRLVAIDLDNTLLRSDLSISSTNRNALKRAREAGVVIAIATGR
                     MFQTASLILSDLGPDMPVAAYNGALIRMRDSGETLCSMPIQPDDGERVVRFLWDFDVA
                     FQAYFDDQLYVPRLTSASAYYSSRYGAAVHVLSDIDEFARRPSLKYLIIEDPGRIDSV
                     RQALEGVIGPNLRTMRSQPGMLEIVDSRVSKGRALRHLASHFSIDIAHTMAIGDSEND
                     IDMLREAAIGVAVSGADHDVKAVADYVVASNDNDGVAEALDMFVSC"
     gene            7357..8181
                     /gene="cdaA"
                     /locus_tag="PLM74_04160"
     CDS             7357..8181
                     /gene="cdaA"
                     /locus_tag="PLM74_04160"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HON42312.1"
                     /translation="MTALSFPDVTPMQVLTTIADVAIVAFLVYQVMTLFKGTQAVSLI
                     KGLAILFAANAVAGLLKLDALHWLLRQVTTMAFVGIPIVFQPEIRKGLDRLGRGRLFG
                     TPMSAIGPEETRRVLAEVCRAAEAMSREYTGSLIVMERETGVGDVAESGVKLDAHVSA
                     EILLNIFTPHTPLHDGAAVIRGNRIVAAACILPLTDRRAPGRKVGTRHRAALGISERS
                     DAVVVVVSEETGAISLASGGELARDLAPAELRGRLDMLFDVNGKEGKFWRIRGAGR"
     gene            8178..8906
                     /locus_tag="PLM74_04165"
     CDS             8178..8906
                     /locus_tag="PLM74_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42313.1"
                     /translation="MTSTARGWREISMRALALGLAAVIWVVAGSEKSRIASTPTFEVI
                     RDARIVVKNVPQSMVVTEVPSSVTVRLRGTRGLETTAGDAEIIAVVNMAGVTPGQHLL
                     AAEPLPPSGLEVVRSGPIMVGVTVEELVSAEFEVEAVIAASLAPEGMAIANTAPPVVR
                     VEGPKEAVGSVSRVVALALSRVEEAPAEGEGQGEDRAEGQAPPAGLMAETQSGAVAVL
                     LALDDNGEVVQGVEVYPGAVLVRW"
     gene            8949..>9608
                     /locus_tag="PLM74_04170"
     CDS             8949..>9608
                     /locus_tag="PLM74_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012811906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HON42314.1"
                     /translation="MGVLFGTDGVREVANTRLTPELAFRLGRAGAAIARSHNNGRPLL
                     VVGGDTRRSTGMLEAAVVAGITSAGADAALAGVIPTPAVAYLTGALGADAGVVVSASH
                     NPAEYNGIKFFGPDGFKLADELEDTIEAGALAAGGGDMGAATWREADGLPRPHGTGVG
                     VVSDIVDAVDMYVRFAVGATRRRLDGLHVVIDCANGASFRTSPAAFRALGARTTVIND
                     SP"
BASE COUNT         1943 a         2552 c         3198 g         1915 t
ORIGIN      
        1 ggtggaacac gacgaggaga ccatcaggtc ggcagattac gttatcgaca taggtcctgg
       61 cgccggcgcc catggcggcg aggtgatagc ggcgggcact gttggcgaag tccagaggaa
      121 tccggcctcg ctgacaggcc agtacctttc aggagctcgc accatccctg tgccgccggc
      181 gaggagatcg cccaacggga agtcgctggt gatcaagggc gctcgcgagc ataatctcaa
      241 gagcctagat gtgtcgatac ccttggggct gttcgtatgc gtgacgggcg tttccggatc
      301 cgggaagagc accttgatca acgagatact ccacgcgcgg ctcgcgcacg acctgaaccg
      361 ggcgcatgcc aggccgggcg cgcacgacga catactgggc attgaatacc tcgacaaggt
      421 catcggcatc gaccagtcgc cgataggtcg gactccaaga tccaacccgg ccacctacac
      481 cggtgtgttc gatctcatca gagaggtatt tgccagaacg accgaggcaa gggcgaaggg
      541 ttacaagccc ggcaggttca gtttcaacgt caagggcggt cggtgcgagg cgtgccgggg
      601 cgacggcatc atcaagatcg agatgcactt cctgcccgac gtgtacgttc catgcgaggt
      661 gtgccatggc gcgcggtaca acagggagac cctagacgtg aagtacaaat ccaagagtat
      721 cgcagacgtg cttgccatga aggtcgagga agcattggag tttttctaca acgttccgag
      781 gatccggcgc aagctgcaga ctctttggga tgtaggcctt ggctatattc agctgggcca
      841 gccggccacg actctttcag ggggcgaagc ccagcgcgtg aaactcgcca ctgagttatc
      901 acggcgtact aacggcaaga cattgtatat attggacgag cccaccaccg ggctgcattt
      961 tgcggacgta ggcaagctcc tagaagtgct gcagaaactg gtggacgggg gcgatactgt
     1021 gctggtaatc gagcataatc tggatgtcat caagactgcc gaccacatca tagatcttgg
     1081 ccctgaaggc ggcgacaggg gcggacgcct gatcgccgag ggcaccccgg agcaagtcgc
     1141 agccattcgc gagtcatata cagggcaatt cctggccggg attctgcgga ctgaggcaaa
     1201 atccgagcat gggctggagg cgatgaccgg tgccgttgga tgataccctt gccggccttc
     1261 ccacgtctcc aggcgtctac ttgatgaagg atgatgcggg gcaggttatc tacgtgggca
     1321 aggccataaa cctcagaaat cgagtccggt cgtactttca atcatctcgc aaccattcgc
     1381 ctaaggtttc gcgaatggtg gagcgaatcg ccgacataga ctacttggtc accggctcag
     1441 agctggaggc cctcatactc gagtgcaatc tcatcaagaa gcacaggccg cactacaatg
     1501 tcaggcttcg cgacgacaag acgtacccgt tcatcagggt gactgtgaac gagccttatc
     1561 ccaggatctt catgacccgg acagtcgtga gggatggttc gcgttacttc ggtccctaca
     1621 ccgacgtccc ggccgtgcgc gagacgctac ggatgctgaa aaccctcttc ccgctaaggc
     1681 agtgttcgcg gaagttcgac ccggagtccc ccgcgcgccg tccttgcctc aactttcaca
     1741 tccggcgctg cctcgggccg tgtagcgggc aggtggaacc ccgtctctac cgagagatgg
     1801 tcgacgaagt tgtgctcttc ctggaaggta ggcaggcgga cttgatcgag agtctccgca
     1861 ggcgcatgga ggccgcagcc gacggcctcg attttgaaca ggccgcccga cttcgcgatc
     1921 agatgcgcgc tgtagagaag gttgtcgaga agcagaggat cgtctccgat gcaggcgttg
     1981 accaggatat aatagcgttc gccaccgatc cctggggcgc gtgtgtgcgg gtaatgttcg
     2041 tccgcgacgg caagatcgtg ggtagccagc acttcatgct gggcggcgcc gacggagccg
     2101 acgacgccga gatgctgtcg gccttcatca aggaatacta ctcggaggac tctccggttc
     2161 cccgggaatt gctcctgcag gcagcaatag acgatgcaga tatcctagaa aagtggctgg
     2221 ccggtcgtcg cgggggggcc gtgtcggtta aggcgccgca gcgtggcgcc cgtcgcaagc
     2281 tggtggaact ggccgcccag aatgcccgtg agtacctcac acgtgcgcgc gcggcagaac
     2341 agagcgagat gcgcacgcgc atggacggcc tggctgaact tgcggcctac ctgaagctgg
     2401 gtgcgcctcc cgccaggata gagtgttacg acatatccaa tctgcagggc actgatgctg
     2461 tgggatctat gatcgtgttc gaggagggac gtccggcaaa gtcgcaatac aggcggtacc
     2521 gtatccgcca tgtggatgga caggacgatt tcgccatgat gggcgaggtc attaggaggc
     2581 gactggcccg tgcgaccggg gagaaccctg atccgcgctt cgggcgtctg cctgatctca
     2641 ttgttgtgga cggtggcaaa ggccagctca attcggcagg cgctgctgtg gatgccatgg
     2701 cccaatcggc aggattaccg agagttcccg tagtcagtct ggccgagagg aatgaggaga
     2761 tcttcttgag aggcgaatcc gcgccgctca tgctgcctcg agagtcgaag tcgctccgtc
     2821 tcatgcagca tatgagggat gaggcgcaca ggtttgctgt agcataccat cgggccctga
     2881 ggggcaagaa gggaatcgcg tctgaacttg accaggtacc gggaataggc aagaagcggt
     2941 tggtggccct gatgaagagg ttcgggtcgc tggaggcgct ggcaagcgct tcggaggatg
     3001 aactcgtctc agtccacggc atgacggccc cggcagccag ggccgtgtgg gagcatcttc
     3061 atccacagga gggaacatag atgaatgcgc gaattagcgg cggcgctgcc aaaccggcgg
     3121 ccgtgacagc gacggtgaca gcgacggtcg acagtcgtat cggccaggtg cgggctgagg
     3181 caggcgtcgg agggctcagg cgagtcctgc ttccccaagt ggatacctct cgactgctgc
     3241 acttgccctt gcccggtccg atcattcgta ctctggcagg gggtgcgctc acgccagcga
     3301 cactcctgtc gaacctgggt ctgaatcagg cgatgatcgg cccaaaggag ctcgcggcag
     3361 ggccaaacag aatctcgcga tccggttctc gttgggcagg cgcggatccc cgggacgttc
     3421 tgacgtctgt gctgcatgac ctaaaactct tcttcgacgg cgacctggat cctcgaggac
     3481 tcctctttga ttaccccatt gacctcacgt ggtgtagcca cttcacccaa cagatcatga
     3541 ccggcatggc tagtattccg ccggggacgg tcacgaccta cggagcactg gcgacggcgg
     3601 ctcaaagccc gcgcgcagcc cgggccgcag gatctgtggt cggcgccaac cccctggggg
     3661 tagtagtgcc gtgccatcgg gtgatcgggt ccaacggggc cttgacaggc ttcggtggcg
     3721 gattgccgct caaggtggca ctgttggcct tggaagagac cgcggttaca caaagttaat
     3781 gcacgtattg acattatcaa catgtggctt tatgatgtga attaggagct atgccggacg
     3841 agggaggtgt gccttcaatg aagcgacgtg agcttctagg caaatgcccg gtgtgcgggg
     3901 agggcctgga cgtgaccagg cttgcatgca gaagctgcgg taccgccata gaggggcggt
     3961 ttgatacatg taagttctgt cgactcacgc ctgagcaccg cgcgttcgtg gacgtgttca
     4021 tcaagaacag gggaaatatc aaagaggtcg aaaaggagtt gggaatatcg taccctaccg
     4081 tccgcaacag gctggatgcg gtcatagccg cgctgggcta cagggtggat gaacctgcga
     4141 tggagaaggg cgatgacggt gctcaccgca aggcgattgt ggaagctctg gcacgcggcg
     4201 agatcagcgc cgacgacgcg gttaagcaac tccgggtcaa gtgatcaggg gaggacggag
     4261 caatgagcga agagcggaag attatcctga acctactggc ggagggcaga atcactgccg
     4321 atgaagccga gcagctatta gaggcattgg acgaatccga cctgtacggc atgggcgatt
     4381 ccggcacagt cgatgaggtt gatcaggtcg gtccggccgg tcaggtacac caggctgatc
     4441 agggcgagtt gggcagccgg ctggaggccc gcgttgatgt tatcccggac gatctttccg
     4501 agaggctttc gaaggcggtg tcggaaatgg ccgaggccgg ccaggagttg cccgacaggc
     4561 tggcgcggat gttgggctcc atgtttggct cgttcggctg ggcgttcggc cccgtcggag
     4621 tccaggccga gcagacgttt gagtccgaaa tgcctgcaga ttgcgtcata tccgtcatcg
     4681 acttcgcaac ctcaaacggc agtgtcaagg tgacgggctg ggataggtca ggttacaaag
     4741 tagtcgctag ggcaatggtg aggggcgcgg aaaccaaatc cgaggcggag cgtaggttgg
     4801 cttcggcgct ttcgctttcg cttgagggcc aagtgatgcg gcttgaatgc accgatggca
     4861 cccttagcaa ctcgctctcc atcgaagcgt acgtgcctag ggcgaataca tatgatgtca
     4921 aggtgagttc gcgcaacggc tctgcgaagc tggaattgtt gaccgccggg tcggtcgatg
     4981 tgcgctcagc caacgggcgc gtcgtacttg aagatattac ggcggcgaag gtcactgcgt
     5041 ctgcgcgcaa tggatctgtg acagcatccg gggacctggg cgatgctagc cttgaaacag
     5101 ccaacggctc tgtgagcgtt gtcttgtcat acaggtccgc cggaaggctc cggatgaaca
     5161 ccgggaatgg cagcgtgcgg ctgaacgttc ctcgtgatgc cggcgtaatc tatgagatca
     5221 gcgcacacac ggtcaacggc tcggtcagga cctctctaga aggcgccgaa gtccggcgcg
     5281 aacggggttc tgggagagac gcgggccgga ccatgtctgt gcatacgcgt cccggtagcg
     5341 ccgtgccact gccccccgtt gttgcggtgg aagcccgcgc gctcaatggt tcggtatcgg
     5401 tgcacagcgt ctaggatgaa gtttcagggg agggttgagc agtggaagca gagaagagac
     5461 agattcttga gatggtgcgc gacggcaaac tgactgtgga agaagcgcag aagataatgg
     5521 atgccatgga gcagggagac gaaggcatgc ctccggctac tgcgactaga ccacccagat
     5581 ttctgcgtgt tcgggtgaaa gacgaggatg ggtcgaaggt caacgtcagt gtgccgatat
     5641 cgcttgtgcg tgtactgtgg aagtttatcc ctcgcgatgc catgcgcgag ctggaagggc
     5701 acaacatcga catcgacgcc attctcgcag cagtcaatga gggcgcgcag ggaaacctgg
     5761 tcgatgtaga agatgaagac ggaacaaagg tcgagataat cgtggagtag ggcgttgtac
     5821 cgaaggtagt agaggattct tggcagaatg ctagaataat atcaagatga atgcaggccg
     5881 actgggggtg tgggcagtat gcagaggcgt ctgtataggt cagtgcgcga acgcatgctt
     5941 gggggcgttt gcggcggaat cgccgactat ttgggaaccg atccaacact catacgtcta
     6001 gtatgggtag cactctccct gttcaccaga gggttccctg gagtcatact ttacatcgtc
     6061 ggatggatcg tgataccgga ggaacccgtt tgattactct gtggaaggct ttgcattcag
     6121 atggcaggta actacaatga tcgcccccat ggcacgatgc cgggttggct ggtgaaatgg
     6181 ctcatcaatg cgctggcgct gctgatagtg tcgtacgttc cctatggccg cagccagctc
     6241 atacacatcg acggcgctgg agcggccctt gtagctgcga tggtattggg catcgtcaat
     6301 acattcatca ggccgatatt catattcctc agcctgccga tgcagttcgc caccttcggc
     6361 ctgttcactc tggtcatcaa tgcgctcatg ctcatgctgg gcgattggct gatgggttcc
     6421 tcgcttgacc tgcccggagg gctgtttgcc gccatcattg cctccgtcct gctcggcctg
     6481 accggcgccg cgctcaatgc gatcatccga ggagacaagt agccattgat acgtcttgtg
     6541 gccatcgatc tggacaatac gttgcttcgc tcggacttgt cgatttcttc tacgaaccgg
     6601 aacgctctga agcgggcccg cgaagccgga gtcgtgatcg cgatagcgac gggcaggatg
     6661 ttccagaccg ccagcttgat actgtctgac ctggggcccg acatgcccgt agccgcatac
     6721 aacggcgcat tgatcaggat gcgagacagc ggcgaaacgc tgtgcagcat gccgatacaa
     6781 cctgacgatg gcgaacgcgt cgtgaggttc ctgtgggact tcgacgttgc gtttcaggca
     6841 tacttcgacg accagctcta cgtgccgcgc ctcacatcgg cctcggccta ctacagctcc
     6901 aggtacggcg cggcggtgca tgttctgagc gatatcgacg aatttgcgag gcggccgtcg
     6961 ctgaagtact tgatcatcga agatcccggg aggatcgact cggtgcggca ggcgcttgaa
     7021 ggggtcatcg ggcctaacct gcgcaccatg aggtcacagc ctgggatgct tgagatagtt
     7081 gattcgcgtg tgtcgaaagg gcgggcactc cgtcatcttg ccagtcactt tagcatagat
     7141 atcgctcaca cgatggccat cggcgactcg gagaatgata tcgacatgtt gcgcgaggcg
     7201 gcgatcggag ttgccgtgtc aggcgccgac cacgacgtga aggcagttgc cgactatgtg
     7261 gtggcttcga atgacaatga cggtgtggcg gaggccctcg acatgttcgt atcgtgctga
     7321 gtcggcctga ttcgattccg ttggggagtg actggattga ccgcgctgag ctttcccgat
     7381 gtgactccga tgcaggttct gaccactatt gccgacgtgg cgatagtggc tttcctcgtc
     7441 tatcaggtta tgacgctctt caagggcact caggcagtgt cgctaatcaa gggactggcg
     7501 attctgtttg ctgccaatgc tgttgctggg ttgctcaaat tggacgctct gcactggctg
     7561 ctcaggcaag tgacaaccat ggcgttcgtg ggaatcccca ttgtctttca gcctgagatc
     7621 cgcaaaggct tggatcggct tgggcgcggt cggctgtttg gcacgcccat gagcgccatc
     7681 ggccctgagg agactcgcag ggtgttagct gaagtgtgta gggccgccga ggcgatgtcg
     7741 cgggagtaca ccgggtcctt gatcgtcatg gaaagagaga ccggcgtggg tgatgtagcc
     7801 gaatcaggag tgaagctgga cgcgcacgtc tccgcagaga tattgctgaa catcttcacc
     7861 cctcatacac ctctccacga cggggcggct gtgatccgcg gaaatcggat agtagctgcg
     7921 gcttgtatct tgccgctcac ggataggcgt gctcctgggc gcaaggtcgg caccaggcac
     7981 cgtgcggcgc tgggcatcag tgaacgtagc gatgcggtag ttgtggtcgt gtcggaagag
     8041 acaggggcga tctctttggc gagcggcggt gagctcgctc gcgatcttgc gcccgcggag
     8101 cttcgagggc ggcttgatat gcttttcgat gtcaatggca aagagggcaa gttctggcgg
     8161 ataaggggcg cagggcgatg acatcgactg caaggggctg gagagagata tccatgaggg
     8221 ctctcgcgct cggcctggcg gcagtgatat gggtagttgc cggcagtgag aagagccgca
     8281 tagcgtctac acccacattt gaggtcatcc gagatgccag aatcgttgtc aagaatgttc
     8341 cccagagtat ggtagtgact gaggtcccgt cctctgttac agtgcggctg aggggaacta
     8401 gggggctcga aaccacggcg ggcgacgctg agattatcgc cgtggtcaat atggcgggtg
     8461 tgactcccgg gcaacatctg ctggctgccg agcccttgcc cccttccggg ctggaagtcg
     8521 taaggtccgg ccctatcatg gtaggtgtga cagtggagga attggtgtcc gctgaattcg
     8581 aggtggaagc ggtcatcgcg gcgagcctgg cgcccgaggg catggcaata gcgaacacgg
     8641 cgccgcctgt ggtgcgcgtg gaaggcccga aagaggcagt gggcagtgta tcgcgtgttg
     8701 tggcgctcgc tctgagccga gtggaagagg cgccggcaga gggtgaggga cagggcgaag
     8761 acagggcaga gggccaagcg ccgcctgcag gcttgatggc ggagacccaa tcgggagcgg
     8821 tcgctgtact gctggcattg gacgataatg gcgaggttgt acagggcgtg gaggtgtatc
     8881 cgggggctgt gttggtacgc tggtaatctc gctgagtgcc ctacatggag tggaggtcga
     8941 attgtgcagt gggtgtgctt tttggaactg acggggtgag ggaagttgcc aatacccgac
     9001 taacacctga gcttgcattc aggctgggca gggcaggcgc cgcgattgct cggagtcaca
     9061 ataatggcag gccgcttttg gtagtgggcg gcgatacgcg aaggtccacc ggcatgctgg
     9121 aggcggccgt agtggccggc atcacttccg ctggtgccga tgcagccctg gcaggggtaa
     9181 tacccacacc ggccgtggcg tatctcacag gcgcactggg tgctgacgcg ggcgtggtgg
     9241 tctcggcctc tcacaaccct gcggagtaca atggcatcaa gttcttcggc cctgacgggt
     9301 tcaagctagc cgacgaactg gaggacacga tcgaggctgg ggccctagca gcaggaggcg
     9361 gcgacatggg cgcggcgacc tggagagagg ccgatggcct tccacgcccg catgggaccg
     9421 gcgtgggggt cgtaagcgac atagtcgatg ctgtagatat gtacgttcgg ttcgctgtgg
     9481 gcgccacccg gcgcaggctg gacgggctac atgtagtgat cgattgcgca aatggtgcct
     9541 cctttcgcac ctctcccgcg gcctttcggg cgttgggcgc caggacgacc gtgatcaatg
     9601 attccccg
//
LOCUS       DAOTAC010000065         9538 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_6135_length_9538_cov_4.721713, whole genome shotgun sequence.
ACCESSION   DAOTAC010000065 DAOTAC010000000
VERSION     DAOTAC010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 9538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9538
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6135_length_9538_cov_4.721713"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(205..1218)
                     /gene="dmpG"
                     /locus_tag="PLM74_04175"
     CDS             complement(205..1218)
                     /gene="dmpG"
                     /locus_tag="PLM74_04175"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008701 - 4-hydroxy-2-oxovalerate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HON42315.1"
                     /translation="MTIRKRIHIVDTTLRDGMHAVSHQFTPNQARSIAAALDAAKVEM
                     IEVSHGDGLGGSSVQYGFAAASDEEYLEAVAGAIVNSQLAVLLLPGIGTQEDLKMARD
                     HGAAAVRVATHATEADIGEQHIKLARDLGMRSIGFLMMSHMVSPEKLLEQAKLMESYG
                     AEVVYVADSAGAMLPEDVRARVGLVKENLGVDVGFHAHNNLMLAIGNTLAAIESGATY
                     VDATLRGLGGGAGNSPTEVLAGVLDRAGYDTGMDFYKAMDAAEQVVEPLMHRPQQVSN
                     ASLMLGYAGVYSSFLLHTHRAAERFGVDSRDILVELGRHRIVGGQEDTIIDVAYGLSK
                     ASR"
     gene            complement(1215..2096)
                     /locus_tag="PLM74_04180"
     CDS             complement(1215..2096)
                     /locus_tag="PLM74_04180"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003542209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HON42316.1"
                     /translation="MDRVGVAIVGPGNIGTDLMYKVLRSRRMELRMMSGIVPTSDGLS
                     RAKQLGIGTTSDGIGPVLDDPEIAIVFDATSAKVHLEHAPLLERAGKFAVDLTPASVG
                     PFVVPAVNAQVHVNTRNVNMVTCGGQATVPIVAAISRVARVRYAEIVATIASKSAGPG
                     TRQNIDEFTETTARAICVVGGAEKGKAIVILNPAEPPIIMRDTVYAIVDDPDGEQIRA
                     SVREMVATVQSYVPGYRLKFDPLVEDDKVTVMVEVVGAGDYLPTYSGNLDIMTAAACA
                     VGERLAENLLQKGDCKA"
     gene            complement(2124..2894)
                     /locus_tag="PLM74_04185"
     CDS             complement(2124..2894)
                     /locus_tag="PLM74_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HON42317.1"
                     /translation="MIRTLSTQLIDAELQRRPIAQITADLPQMTIEDAYAVQTETIRR
                     KMRSGEKIVGKKIGLTSKPMQTLLGVSQPDYGILLSSMVVDEFEPVDSSELIQPKAEG
                     EIAFILRTDLHGPGITVPDVLRATDAVMACIEIVDSRVENWKIRIQDTVADNASSARL
                     VLGGKLVGTRDLDLRLIGMILRKNGEIASTGAGAAVLGHPASAVAWLANQLAQFGVGL
                     RAGEVVLSGAITAAVPVSPGDIVCAEFDHLGAVTARFR"
     gene            complement(2990..3769)
                     /locus_tag="PLM74_04190"
     CDS             complement(2990..3769)
                     /locus_tag="PLM74_04190"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HON42318.1"
                     /translation="MGGRGKQDNIVQSVMRAVDILEAFERRKGELGVSDLARELGLHK
                     STVYGILNTLAQRGLVEQVASTGKYQLGIRLFEMGHRIIEEMDFQSLAAPIMESLVDK
                     YEETCHLVVRNGPDVVYVEKRESARSVRIASKVGTRLPCYCTGVGKALLAEMSDDEVH
                     ELYKGKQLKAFTSSTMTDMELLKGSLRKIREQGYAEDYGEFDESVRCVSAVVKDHTGR
                     PVAAISLSGPALRMDETRMREMALAVREATERLSRQLGGRR"
     gene            complement(3789..5444)
                     /locus_tag="PLM74_04195"
     CDS             complement(3789..5444)
                     /locus_tag="PLM74_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HON42319.1"
                     /translation="MRIVVVGGGAGGASAAVKARRINEDAEILIYDKGPHLSYANCGL
                     PFFLGGEIEKREDLFVVPFSRFDGWFNVDVHPWHSVEEINRKNKTVTVRDIQGGKVFV
                     DSYDRLILATGSQPFVPSIEGLRDGIRNVFTLTTVPDVDAACACLRDDEPQQALVIGG
                     GFIGLEVTEALTMRGVAVTLVERLPQVMPIVDSEFSNHLLGELGRLGVRVILGDTVVG
                     LDVDGDRVTGAVLASGTRVPCSLVMIAAGMRPDVSLPVAAGLDIGVAGGIVVNDRMQT
                     SDADIYAAGDCVESEELLTGRRVLSLLAGPANKQGRVAGANAAGADYVFRGALGTCIV
                     RVGRMTVAKTGLSERDAERANKAHFVSDNYARDHAGCYPGSTELHIKLTVEPDTGRLL
                     GAQVAGESGVDKRIDVFATAIYAGLTVFDLEQLDLAYAPQFSSAKGPEVMAGMAAANI
                     VRGEVRNITSCGLRQILGDPGLQLVDVRTDEEARRGMIPGAVHIPLHELRRRLSELDP
                     ARTTVVYCRTGIRSYIASRILTQRGFADVRNLSGAWLSWIIGN"
     gene            complement(5522..6103)
                     /locus_tag="PLM74_04200"
     CDS             complement(5522..6103)
                     /locus_tag="PLM74_04200"
                     /inference="COORDINATES: protein motif:HMM:NF021252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family (seleno)protein"
                     /protein_id="HON42320.1"
                     /translation="MADKVRCMRRARELAAQYTTKYWGCAQSSFSATLDALREEGVEL
                     VSKEEQDEIFKGLIGLSGGTGNLGGGTCGALAGTAFAISLASGVGRKEQLEDKMNRWI
                     AFDNVAETIGKQFMHEYGGLRCRDVTWNRWGKWWDSWNPVAKAEFAKEERARGCLCKD
                     RCTIALAAGWAVGYILDILDDPRTLEQMKELHG"
     gene            complement(6154..6750)
                     /locus_tag="PLM74_04205"
     CDS             complement(6154..6750)
                     /locus_tag="PLM74_04205"
                     /inference="COORDINATES: protein motif:HMM:NF021252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HON42321.1"
                     /translation="MDSNGNKEKCIQRARELAVEYQTTLVGCAHGSFAATVDALREFG
                     IELVTPEVEDIMFKGLVGLTGGLGNMNTGTCGAVTGSSFAVSLACNITRELTEQQGKG
                     MRWIAYYNVADGVANRFIEKYGSIVCRDIQLRIFGMAFDSRVPGRNKELFGCASKHNC
                     QTPGACTIANGAGWAAERIWDMVHNPKDLTWVISEHEK"
     gene            complement(6773..8440)
                     /gene="ilvD"
                     /locus_tag="PLM74_04210"
     CDS             complement(6773..8440)
                     /gene="ilvD"
                     /locus_tag="PLM74_04210"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HON42322.1"
                     /translation="MQSDEIRKGYHRAWHRSLLKANGYTDEELARPLVGVANSQNEIV
                     PGHKHLDQIAEAVKSGIRMAGGTPVEFPVIAVCDGLAMGHGGMRYPLPSREHIADSVE
                     IMACAHQLDALVLIPNCDKVVPGMLMAAARLDIPSIVISGGPMLAGRLCGEDIDVSRV
                     GEAQGAYAAGRIDDGELLEYENRGCPTVGSCAGMFTANTMNCVTEALGMGLPGNGTVP
                     AVYAERIRLAKIAGMRIVDLLRSGVRPSSIMTREAFENAIAVDMALGGSTNTVLHLPA
                     IAHELGIHIDLDLFDELSEKTPHLCNMSPAGPHHLQDLYEAGGVPAVMKELAQLGLIH
                     EDARTVLGISIGGIAASAAVRRPDVIRPVDNPYHAQGGIAVLRGNLAPECAVVKRSAV
                     AQEAWYHKGPARVFDSEEVAEEAIFGHRINKGDVVVIRYEGPMGGPGMREMQMATAAL
                     VGLGLGKDVALITDGRFSGATRGIAIGHISPEAMAGGPIAVVREGDTIEVDIPGKKLN
                     LLIPDSELQARLDAWRPPTPRVKKGYLNAYSRLATSASTGAVFREQD"
     gene            complement(8500..>9538)
                     /locus_tag="PLM74_04215"
     CDS             complement(8500..>9538)
                     /locus_tag="PLM74_04215"
                     /inference="COORDINATES: protein motif:HMM:NF014500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HON42323.1"
                     /translation="ALTGFLVSIPVFYDVVFVILVPLAASLSQMTKMKFEYYVGALVV
                     GAGIAHCFVPPTPGPLASAAILNVPLGTMIGVGIVIGIPILIASVLVYKVFFVDRPGF
                     WNPAKDVDVDIAADAGSASFDSSKLPGFVASILPIALPVVFILIGTVSQAIYGKEVPA
                     AVKFLSDRTVAMLAGTIAAYVLASTRVSHDQIDKSISKALAASGVVLVITGAGGSLGA
                     VLGKTDIGNVMVAASKSLRLAPVVLAFIVAVLIKLAQGSGTVAMITTAGLIAPMLQQT
                     GSGGLWVAMAASAGGIALGHINDSGFWVTAKTAGLTTSGGLKTYTLACTIVAVVAMVF
                     IVIGQVLFPAL"
BASE COUNT         1880 a         3108 c         2416 g         2134 t
ORIGIN      
        1 aggaccgaga ccaccaatac tagggacttt ctcaacaact gcctctcctc ctcactggta
       61 agtatgccag ggggggccaa ccgaacacgt gtgccagcgc cccccgggta ttgaggagaa
      121 ttatacaggc gcagcgtatg agtgtcaagt gcgaaaaccg gctttgttat gcgtctgtga
      181 taatgtcacc cacgaacaag gtcatcagcg tgaagccttg gacagtccat aggccacgtc
      241 aatgatggta tcttcctgcc cacctactat cctgtgccgc ccgagttcca caaggatgtc
      301 ccgcgagtcg acaccgaacc tttcggcggc tcgatgggta tggagcagga agctcgagta
      361 cactcctgca taccccagca ttaggctggc gttggatacc tgctgagggc gatgcatgag
      421 cggctccact acttgctctg ctgcgtccat tgccttatag aagtccatac cagtgtcgta
      481 gccggctctg tcgagcactc ccgccagcac ttcagttggg gagttgccgg cccctccacc
      541 caacccgcgc agagtagcat caacatacgt ggctccggat tcgattgcag ccagcgtgtt
      601 tccaattgcc agcatgaggt tgttgtgtgc gtggaatccc acgtccacac cgaggttctc
      661 cttcaccagt cccactcgcg cccgcacatc ttccgggagc atggcgccgg ctgaatcggc
      721 cacgtagact acttcggctc catagctctc catcagcttc gcctgttcca gcaacttctc
      781 gggtgaaacc atatggctca tcatcaggaa accgatggat ctcattccta gatcccgggc
      841 gagcttgatg tgctgttcac ctatgtcggc ctcagttgcg tgcgtggcca ccctgactgc
      901 cgctgctcca tggtccctag ccatcttgag gtcttcctgg gtccctatgc cgggcagcag
      961 caacactgcg agttgcgaat ttactatagc tccggcaact gcctctaggt actcctcatc
     1021 tgacgccgca gcaaatccgt attgcaccga tgatcccccg agcccgtctc cgtggctaac
     1081 ctcgatcatc tccaccttcg cagcatcaag cgcagctgct atgcttcgag cctggttcgg
     1141 cgtgaactgg tgactcacag catgcattcc gtctcgcaaa gttgtgtcga ctatgtgaat
     1201 acgcttgcgt atcgtcatgc cttgcagtct cccttctgca ggaggttctc tgccaacctc
     1261 tcgcccacag cacaagccgc agcggtcatg atatcgaggt tccccgagta tgtcggcaga
     1321 taatcgcctg ctccgaccac ctcgaccatg actgtgactt tgtcatcctc aaccagcgga
     1381 tcgaacttga gcctgtatcc ggggacataa gactgcaccg tcgccaccat ctcgcgcact
     1441 gaagccctga tctgctcgcc gtcggggtca tccacaatgg catagaccgt gtctcgcatt
     1501 atgattggcg gttcagcagg attaaggatg actatggcct tgcccttctc cgctccgcca
     1561 acaacgcata tcgccctagc ggtggtttca gtgaactcgt ctatattctg cctcgtgcct
     1621 ggcccggcgc tcttgctcgc tatcgttgcg actatctctg catacctcac cctagcaact
     1681 ctgcttatcg cagccacaat tggcacagta gcctgtcctc cacacgtgac catattcacg
     1741 ttacgagtgt tcacgtgtac ctgggcgttt accgcaggga ccacgaatgg ccccacagat
     1801 gccggtgtga gatctacagc gaatttgccc gcgcgctcca ggagcggagc gtgctctagg
     1861 tgcacctttg cactggtagc atcaaaaaca atcgctatct ccggatcgtc caggaccggt
     1921 ccgattccgt cggatgtagt tccgatgccg agctgcttgg cgcggctaag accgtctgac
     1981 gtggggacta tgcccgacat cattcgcagc tccatgcgcc tgcttcgaag taccttgtac
     2041 ataaggtcgg tcccgatgtt cccggggccg acaattgcga ctcctactct gtccaaagca
     2101 ttagcctccc aaagagatct cacctatcgg aagcgtgccg tgacagcccc cagatgatca
     2161 aactcggcgc acacgatgtc gcctggcgac actggcactg cggcagtaat cgcccctgaa
     2221 agcaccacct cgccggctct aaggcccact ccgaactgag ccagctgatt ggcgagccat
     2281 gccacggccg aggcagggtg gccaagcacg gcggcgcccg ctcccgtcga ggcgatctcg
     2341 ccattcttgc gcagtatcat cccaatcagc cgcaggtcaa gatcgcgagt gccaaccagt
     2401 ttgccgccga gcaccaggcg tgcacttgat gcgttgtcgg ccactgtgtc ttgaatgcgt
     2461 atcttccagt tttccaccct cgaatccaca atctcgatgc atgccatcac agcgtcggtc
     2521 gcccgtaaca catcgggcac cgtgatacca ggaccgtgaa gatccgtgcg aagaatgaac
     2581 gctatctcgc cctcggcctt gggctgtatg agttcagatg agtctacagg ctcgaactcg
     2641 tccaccacca tggaactaag caagatgccg tagtcaggct gggagacgcc cagaagcgtc
     2701 tgcattggct tgctcgtgag tcctatcttc ttgcccacta tcttttcgcc ggaccgcatc
     2761 ttcctacgta tcgtctccgt ctgaaccgcg tacgcgtctt caatggtcat ctggggcaaa
     2821 tccgccgtga tctgcgcgat gggtcgtcgc tgcagctcgg catcgatcag ttgggtcgat
     2881 agggttcgga tcatctcctg actaagtcgt gcgcagcagg cgataccctc acgatccaga
     2941 ctgtccattt caggtctcct cctcgctcct ttgtcctgcc atgttgactc tatcgccgtc
     3001 ctcccagctg cctcgagagt cgctctgtcg cctccctgac tgcaagagcc atctctctca
     3061 tccttgtctc atccatgcgc aacgcgggcc cagagaggct gatggccgcg acaggccgcc
     3121 cggtatggtc ttttactaca gccgacacac acctgacgct ctcgtcaaac tccccgtagt
     3181 cctcggcgta accctgctcc ctgatcttcc tgagtgaccc cttaaggagt tccatgtcag
     3241 tcatggtact gctagtaaag gctttcagct gcttgccttt gtacagctcg tgtacttcgt
     3301 catcggacat ctcggccaaa agcgctttgc caacccctgt gcagtaacac gggagccttg
     3361 tgcccacttt cgaagctatc cgcaccgacc gagccgactc tctcttttcc acgtacacga
     3421 catcgggccc gtttcgcact acgagatggc aggtctcctc gtacttatcc acgagtgatt
     3481 ccataatagg ggcggccagg gattggaagt ccatttcttc gattatcctg tggcccatct
     3541 cgaacagacg tatgcctaac tggtatttcc cagtggaagc aacctgctcg accaggccac
     3601 gttgtgcaag cgtgttcaag attccgtaca cagtgctctt gtgcaaaccg agctctcgtg
     3661 cgagatcgct caccccaagc tcgccttttc tgcgttcaaa agcttcaaga atatctacag
     3721 cacgcatgac tgactgcacg atgttatctt gttttccccg accgcccact actgtaccct
     3781 ccgctacgtt agttaccgat gatccatgaa agccaggctc cgctcaagtt cctcacatcc
     3841 gcgaatccac gttgggtaag aatgcgtgac gcaatatacg acctgatccc tgtcctgcag
     3901 tacaccactg tagttcgggc tggatcaagc tcactcagcc tgcgccggag ttcatgcagg
     3961 gggatgtgca ccgcacccgg aatcatacct cggcgggcct cctcatcggt cctcacgtcc
     4021 accagctgca agcctggatc gccaaggatc tgcctcaggc cgcacgaagt gatattgcgc
     4081 acttctcctc ttacgatatt ggctgccgcc atccctgcca tgacctcggg ccccttggcc
     4141 gacgagaact gaggcgcata ggcaagatcc aactgctcca ggtcgaatac ggttaggcct
     4201 gcgtatatcg ctgttgcgaa tacgtctata cgcttgtcta ccccgctctc tccggcgacc
     4261 tgcgctccga gcagtcgccc tgtgtccggc tcgaccgtca gctttatgtg aagctctgtg
     4321 ctgccggggt agcatccggc gtggtcacgc gcatagttgt cggatacgaa atgcgccttg
     4381 ttcgcccgtt ccgcgtcacg ctctgaaagc ccagttttcg ccaccgtcat ccgcccgacg
     4441 cgcactatac atgttcccag agccccgcgg aagacgtaat cggcgccggc agcgttggct
     4501 ccggccaccc ttccttgctt gttggcaggc ccggcaagca ggcttaggac gcgcctgcct
     4561 gtcagcaact cttcactttc gacacagtcg cccgctgcat atatgtcagc gtcgctggtc
     4621 tgcatccggt cgttcacaac gattccaccg gctaccccaa tatcaagccc tgccgccaca
     4681 ggcaaactca cgtcgggcct cattcctgcc gctatcatca caagcgaaca cggtaccctc
     4741 gtaccgctcg ccagcacagc acctgtcacc cggtctccgt ccacatccaa cccgaccacc
     4801 gtgtcgccca gaatcactct gactcccagc ctgcccagct cacccagcaa gtggttggag
     4861 aactccgagt cgacgatagg catcacctga ggcaacctct ccacaagcgt cacggccacg
     4921 ccccgcattg tgagcgcttc agtgacctcg agcccgatga accctcctcc gatcaccaac
     4981 gcctgctgcg gttcatcatc gcgcaggcac gcgcaagctg catctacatc cggcactgtt
     5041 gttagggtga acacattgcg tatgccatct cgcaggcctt cgatgctggg cacaaaaggc
     5101 tggcttccag ttgccagaat caagcgatcg taactatcga cgaacacctt gccgccctga
     5161 atgtcgcgga cggtgaccgt cttgttcttc ctgttgatct cctcaacact atgccacgga
     5221 tggacatcaa cattgaacca accgtcgaaa cgagagaatg gaacgacaaa cagatcctct
     5281 cgtttctcta tctcgcctcc tagaaagaag gggagcccgc agttggcgta tgacaagtgc
     5341 ggccctttgt catatatcaa aatctccgca tcttcgttga ttctgcgtgc cttcacagcc
     5401 gcgctggcgc ccccggcgcc accgccgacg acgacgattc tcatggcaaa ccttccctca
     5461 ggcggcggga tgcgcccccc aaaccgggac gctccccgct cgccgcagac gatttcactg
     5521 actagccatg cagctctttc atctgttcta gagtcctcgg gtcatccagt atgtccagta
     5581 tataccctac agcccagccc gccgccagtg caatagtaca cctgtctttg cacaggcatc
     5641 ctcttgccct ttcttctttg gcgaactcgg ccttggcaac cggattccac gagtcccacc
     5701 acttgcccca ccgattccac gttacgtcgc ggcagcgcag tccgccatat tcatgcatga
     5761 actgcttccc aatggtctct gccacgttat caaaggcgat ccaccggttc atcttgtcct
     5821 caagctgctc tttgcgcccg actccggaag ccaggcttat cgcgaaagcc gtgccggcca
     5881 gcgccccgca cgtacccccg cccaggttgc cggttccgcc tgatagccca atgagtcctt
     5941 tgaatatctc gtcttgctcc tctttggaga ctagctcaac gccttcctcc ctgagggcgt
     6001 ccaatgtggc gctgaagctg gattgtgcgc aaccccagta cttggtggtg tactgcgctg
     6061 cgagttctct tgcgcgccgc atgcatctta ctttgtcagc cacgtcgatc tccctctctc
     6121 tcggctttct cggcgattct tggtgtgcaa cttctacttc tcgtgttccg aaatgaccca
     6181 tgtgaggtct ttaggattgt gcaccatgtc ccatatgcgc tctgcggccc atcccgctcc
     6241 attcgcaatg gtgcacgctc caggtgtctg acaattgtgc ttgctcgcgc atccgaagag
     6301 ttccttgttc ctgccgggaa cgcgtgaatc gaacgccata ccgaatatcc tgagctgaat
     6361 atcgcgacag acaatcgagc cgtacttctc aatgaacctg ttggccacgc cgtcagccac
     6421 gttgtagtag gcgatccatc tcatgccctt gccctgctgc tcggtgagct ctctggtgat
     6481 attgcatgcc aagctaaccg cgaaagagga ccccgtcacg gcgccacatg tgcccgtgtt
     6541 catgtttcca agtcctccgg tgagcccgac gagtcctttg aacatgatgt cttctacctc
     6601 aggggtgacg agttctatgc cgaactcacg gagggcgtcg actgtagctg cgaatgagcc
     6661 atgcgcgcat ccaaccagag tggtctggta ttccacagca agctctcttg ccctttggat
     6721 gcacttctcc ttgttgccat tactatccat gattgtccac ttcccctcag tactagtcct
     6781 gttctcgaaa taccgcaccg gtgctggcag atgtagcaag cctcgaatac gcgttcagat
     6841 agcccttttt caccctcggt gtcgggggtc tccatgcatc gagcctggcc tgaagttcac
     6901 tatcgggtat cagcaggttg agtttcttcc ccgggatgtc tacctcgatc gtgtcgccct
     6961 cccgaacgac cgcgatcggt ccgcccgcca tggcctcagg agagatgtgg ccgatagcga
     7021 tgcccctggt agcgccggaa aaccgaccgt cggttatgag cgctacgtcc ttgcccaatc
     7081 ccagcccgac caaggcggcc gtcgccatct gcatctcccg cattccgggt ccccccatcg
     7141 ggccctcgta gcgaatcaca acaacgtctc ccttgttgat cctatgtccg aagatggctt
     7201 cctcggccac ctcttccgag tcgaagaccc tggccggtcc cttatggtac cacgcttctt
     7261 gagccaccgc gctccgctta actacggcgc actcgggggc caagttgcct cgcagcaccg
     7321 caatgccgcc ctgcgcatgg tagggattgt caacgggcct gattacatcg ggtctgcgca
     7381 ccgcggcgct tgcagcaatg cccccgatgc ttatcccgag cacggtacgt gcatcctcat
     7441 gtatgagccc cagctgagca agttccttca tcaccgccgg tacccctccg gcttcgtata
     7501 gatcttgaag gtggtgcggt ccggccgggc tcatgttgca cagatgcgga gtcttctcgc
     7561 tcagctcgtc gaacaggtca aggtcgatgt gtatgcccag ttcatgggcg atcgccggaa
     7621 gatgtaagac cgtatttgtg gagccaccta atgccatgtc gaccgcgatg gcgttctcga
     7681 atgcttcccg agtcattatg gacgacggcc tgacgcccga gcgcagcaag tcgactatcc
     7741 tcatgccggc aatcttggca agtcggattc tctcggcgta cacagccgga acagtgccgt
     7801 tgcccggcag gcccatcccc aatgcctcag tgacgcagtt catggtgttg gcggtaaaca
     7861 tgccggcaca cgatccgaca gtaggacagc ctctgttctc gtactccaga agctcaccgt
     7921 cgtcgattct gccggcggca tatgctccct gcgcttcgcc tactctgctc acgtcgatgt
     7981 cttcgccgca cagcctgccg gcgagcatcg gaccgccgct aatcacgatt gacggtatgt
     8041 cgagcctggc agcggccatg agcattcctg gcacgacctt gtcgcaattg ggtatcagaa
     8101 caagtgcatc tagctgatga gcgcaggcca tgatctcaac agagtcggct atatgctccc
     8161 tgctgggcaa agggtatctc atgcctccgt gccccatagc caagccgtcg cacacggcga
     8221 tcaccgggaa ttccaccgga gtcccgcctg ccatccgtat ccccgacttg acggcctctg
     8281 cgatctgatc caggtgtttg tggccgggca ctatctcatt ctgcgagttg gccaccccca
     8341 cgagcggacg agctagctcc tcatcggtgt agccattcgc cttgagcaag gatctgtgcc
     8401 atgctctgtg gtagcccttc ctgatctcat cgctctgcat ttcatgacct ccgttcaatc
     8461 gggcccgacc ggcctaatcc cgggagcccc aaccggttcc tatagcgccg ggaagagaac
     8521 ctgcccgatg acaatgaata ccatcgcaac aaccgccact atggtgcacg cgagagtgta
     8581 agtcttgagt ccgcccgagg tggtgagtcc agctgttttg gcagtgaccc agaaaccgga
     8641 gtcgtttatg tggccaaggg cgattccgcc ggcagatgcg gccatcgcta cccacaatcc
     8701 gccgcttccc gtctgctgca acatcggcgc tatgaggccg gcggtggtaa tcatcgcaac
     8761 ggtccccgac ccctgcgcga gcttgatgag gacagccact atgaaggcca acaccactgg
     8821 cgcaagcctg agcgacttgc tcgcagctac catcacgttg ccaatgtcgg tcttgcccaa
     8881 gaccgcaccg agcgatccgc ctgcgcccgt aatcaccaac acaacacctg acgcggccaa
     8941 tgccttgctg atggatttgt caatctggtc atggcttacc cgagttgagg ctagcacgta
     9001 tgcggcaatg gtgcccgcaa gcatagccac ggtccggtcg gataagaatt tgaccgcggc
     9061 tgggacttct ttgccgtaga tcgcctggct tacggtgccg atcaaaatga acactactgg
     9121 aagagctatc gggaggatgg aagcgacaaa gcccggcagt ttgctggaat caaacgatgc
     9181 gcttccggca tcggctgcta tgtcgacgtc gacatcctta gcggggttcc agaatcccgg
     9241 cctgtcgacg aagaagacct tgtacaccag aactgatgct ataagtatgg gaatgccaat
     9301 cacgatgcct acgcctatca tcgtgcccag gggaacattg aggatcgccg ccgaggccaa
     9361 aggtcctggc gtcggaggaa cgaagcagtg agctattccg gcgcccacca cgagagcgcc
     9421 aacatagtat tcgaacttca tcttggtcat ctgcgatagg ctagccgcca gtggcacgag
     9481 aatcacgaag accacgtcgt agaagaccgg aatggacact agaaagcctg ttaacgcc
//
LOCUS       DAOTAC010000066         9437 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_6200_length_9437_cov_4.938499, whole genome shotgun sequence.
ACCESSION   DAOTAC010000066 DAOTAC010000000
VERSION     DAOTAC010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 9437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9437
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6200_length_9437_cov_4.938499"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="PLM74_04220"
     CDS             <1..342
                     /locus_tag="PLM74_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015044812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HON42324.1"
                     /translation="QGGTNLSGGQKQRIAIARALVKRPKVYVFDDSFSALDFATDARL
                     RAALTSEVRDTGATVLIVAQRVSTIMNADRIIVLDKGVIVGMGTHEELMEHCAVYREI
                     VFSQLAEEEVA"
     gene            405..2357
                     /locus_tag="PLM74_04225"
     CDS             405..2357
                     /locus_tag="PLM74_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013731551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON42325.1"
                     /translation="MRGMGPGGRPGGRGHGPMGAMMPTEKAKDFKGSLRRLAGYLKPH
                     KNQVAAVMVMALISVGLSIVGPKILGRATNLLFEGVIGKQLPPGLTKESIIAMLKAVG
                     KDRMADMVSGMNIVPGRGVDFAAIGRVLLTLAGVYLASALFGWMQHYIMAGVSQRTVY
                     GLRKEADAKLARLPLKYYDSNTRGEILSRLTNDIDNIASSLQQSLNQLTTAAFTVIGV
                     LIMMFSISPLLAAIALATLPLSVLVTLMIAKRSQKQFAAQWESTGALNSHIEEMYTGH
                     NIVKVFNRQQTAVDAFDKENERLFQAGFKAHFISSTIQPGMQFINNLNYVAVCVIGGM
                     KVASGALSLGDVQAFIQYSRQFTQPIVQTASIMNILQSTVASAERVFQLLDEQEEVPD
                     TANPVKLEHVAGHVVFDNVSFKYKAETPLIEHMHLDVQPGQTVAIVGPTGAGKTTLVN
                     LLMRFYEIDEGSITIDGVDIRRMTRNSLRSLFGMVLQDTWLFAGTIRDNIAYGRENAS
                     EQEIMAAARAAYVDHFVRTLPDGYNTKLDDDASNISQGQKQLLTIARAFLADPEILIL
                     DEATSSVDTRTEVLIQEAMSELMKDRTSFVIAHRLSTIRNADTILVMDNGSIVEQGNH
                     KALMAKRGFYYDLYNSQFAGAYDEAV"
     gene            complement(2358..3878)
                     /locus_tag="PLM74_04230"
     CDS             complement(2358..3878)
                     /locus_tag="PLM74_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42326.1"
                     /translation="MRRRAGAIVIAALTLCVLCAAGGPPADAEQGGMQGGGVPSLGDT
                     LVPTSISAGLTALDQETASIIGPAAKVTGAVNNLLDSMHTFLLMMGARLDVEIQYEPM
                     DAPQAIHLRQSPLERRGDRRIRIDVTVSYQDDVEGRIPLPSVRRWLGSGLPKKGDRLA
                     GVAVRFRFPKSLTCYLEVPLECFDTAIPAWIKRTNENGMATIFLYPKLDQPLGGIREI
                     AEGSIEIDVPMGLSKGFSLNPFDMAIVRPLGDEIRRAVSAWRTVQIERHKPTNWVGSI
                     TLQREITAAWSAERPGPSRLHEGWFVSDKGRSKWTEEVTVNGIRLNDEGLGSGSFAYM
                     VAANIAGDSVHESIAECRSGPRKLTGTCEYNGAMSDANQGSANIELRVDHRGAGPEYT
                     VVLGTRAVQPSPWGFGLSMGGAIKHTSESCDGTSKWTDKFDINDSSVSSAATIPATKL
                     VSSYDHFRSMWKPFDPDAPTLQGSDSWYDDPVTSPDGSVTCTVRNTLTWSLRRVSD"
     gene            complement(3875..5275)
                     /locus_tag="PLM74_04235"
     CDS             complement(3875..5275)
                     /locus_tag="PLM74_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42327.1"
                     /translation="MAYESTAWHSSTEKAQRIALTCAVLLALGVFVTGGFSHAAATAA
                     TPQGEALRLARALHGATTAKEAESVIREIMTALSIPVLRPDMGLDWDDAETPAAGLFE
                     AEIAILARAFVAGSYMSLEELVQSWKDAGIGFEALPGEACTSANVESILRDVRRRAEG
                     LPGDWSGLVIRLIDALGDRDEYPFSLLGQAEQDEGAPRALAGSMQELAVTKAQEIAQG
                     TIGATVRDAQSEPGLDAAAFESTLSSKESMQQMVEGLMGGDIWDFMDPILQASQAAGE
                     DTLSADEVRSILSMVASMTHAASQQAGASLTPEAMASIQARQHHVEVLQAGKLPAVTE
                     TGEAETRANLRLMASLASEGLENARADYENDPERAVDARAAIGAAASLAGAHASADVA
                     QRNLESGEELIDSAERRRQAEQEGEAPPMAGLAISLDEKPMSRLVLDPVQVWLISMDI
                     AGAVRPDLSLLGGGRR"
     gene            complement(5350..5679)
                     /gene="csaA"
                     /locus_tag="PLM74_04240"
     CDS             complement(5350..5679)
                     /gene="csaA"
                     /locus_tag="PLM74_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012810950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone CsaA"
                     /protein_id="HON42328.1"
                     /translation="MAAFDDFQKLDIRIGTVVEATPFPEARVPAIKLIIDFGELGLRR
                     SSAQITRRYTPEALVGRQVAAVVNMPPRRIAGFSSEVLVLGGIPEQGDVVLLAPDRHV
                     ANGTQVG"
     gene            complement(5692..7461)
                     /locus_tag="PLM74_04245"
     CDS             complement(5692..7461)
                     /locus_tag="PLM74_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HON42329.1"
                     /translation="MFITLTPNRPVRILILLCAILAAWSGRPAGAEAPSRDEVEAFYS
                     YDSEAPLAAAQVLLGSSPWYREYEVRYSSLGEIVYARFMVPRSTETMPIAALGSASGS
                     ASSAVPCLVGLHGMFSDSEYQFWSIADFCAKRGIAVMTPSLPYHHKRTEGVPLIPGQQ
                     LIIGPPDAVRENLRRAVVDARRAVDWLAARPDIDQSAISIAGVSLGGIVASLAFKVEP
                     RFANGVFVVGGSGVGGILEHGRTDVLDIFRAAAKANVVNPQDFIDALQIADPINIPDL
                     VPRPALLMNGVSDIIMVMDNALRFRESMSLAEQIWTTGGHYFPMYAAEYLLMDYIAEQ
                     YAASPAFSRGVRIQVGSGFSFTERVRPPKAGADNLWVDVRAELGTPGLVADHQLSVPM
                     VNRAVPIVVMSRETYASLAPFLLGRHLPAFIYIINHDDSLELSAALAYAQVLGLRADP
                     QVYYIGRVATAASGESRDSRKESQWGEGGQGGYGGCGGNGGQESCFVGALSLMQVGYA
                     RSVLESIVRVPHALARPESVPVPLSIFANTQSLGGPGAEEAVGSWLAQATAVSRLKPI
                     PWFPDYPHDNPRWATENPRDGER"
     gene            complement(7647..8516)
                     /locus_tag="PLM74_04250"
     CDS             complement(7647..8516)
                     /locus_tag="PLM74_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013382591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HON42330.1"
                     /translation="MGYEIVTDSSADLTDELIEQYGIHIVSLSFRVGGEEFPCYVQGE
                     KTDYKQFYDRMRKGEMVDTSLIDMSTCRDIFEGILKRGNDVLYIGFSSALSGSYNAGT
                     MVAESLRDAYPERKIVTIDSLSASMGEGLLVYHVAEQRRSGKSIDEVSEWLIANRLHL
                     CHWFTVDDLFHLKRGGRISAATALVGTVLGVKPVLHVDDEGKLVAVGKVRGRRRSLEA
                     LVDEMEKTCANPTEQVVFISHGDCIEDAQYVERLVRERLNVKDVQINYVDPVIGAHSG
                     PGTVALFFLGTAR"
     gene            8684..9127
                     /locus_tag="PLM74_04255"
     CDS             8684..9127
                     /locus_tag="PLM74_04255"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HON42331.1"
                     /translation="MNADELEQHLAHFREVCTNEGLKLTYQRIEVFEEVVGSRDHPDA
                     ETIFNRVRDRLPTVSLDTIYRTLWMLNAVGLITTVGPPREKVRFDADVTPHHHFVCSR
                     CGAVYDINCPELGPVEAPNHIEGVGQVQSVRLELRGLCRQCAGHI"
     assembly_gap    9173..9182
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9251..>9437
                     /locus_tag="PLM74_04260"
     CDS             9251..>9437
                     /locus_tag="PLM74_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011499051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HON42332.1"
                     /translation="MSETTNTPCCDEIVRIPLIGEKAPSFQAVTTQGKISFPDDYKGK
                     WVILFSHPADFTPVCTTE"
BASE COUNT         1987 a         2991 c         2612 g         1837 t
ORIGIN      
        1 cagggcggaa ccaacctgtc gggcggacag aagcagcgca tcgccatcgc ccgagcactc
       61 gtcaaaaggc cgaaggtata cgtcttcgac gacagcttct ccgcgctgga ctttgccaca
      121 gacgcccgcc tgcgcgccgc gctcacaagc gaggtgcgcg ataccggcgc gaccgtcctg
      181 atagtcgccc aacgcgtcag caccatcatg aacgcagacc gcatcatcgt gctcgacaag
      241 ggcgtgatcg tagggatggg cacacacgaa gaactgatgg aacactgcgc ggtctaccgc
      301 gagatcgtgt tttcgcagct tgcagaggag gaagtcgcat gaacaaggaa acacagtcag
      361 cacggttcgc gcagcagtct gtccagtcgc ccgcggagcc aggtatgcgg ggcatgggcc
      421 caggcgggcg tccaggcggg cgtggccatg gccctatggg cgcaatgatg cccactgaga
      481 aggccaagga cttcaaaggg tcgctcagga ggctcgccgg ctacctcaag ccgcacaaaa
      541 atcaggtcgc cgccgtgatg gttatggccc tgatcagcgt gggcctctct atcgtcgggc
      601 ccaagatcct ggggcgggcg accaaccttc tctttgaagg agtaatcggc aagcaacttc
      661 ccccgggcct caccaaggag tccatcatcg ccatgctcaa ggcggtcggt aaggaccgga
      721 tggccgacat ggtgtcgggg atgaatatcg tgcccggccg cggcgtcgat ttcgccgcca
      781 ttgggcgtgt gctgcttacc ctagccggcg tgtaccttgc gagcgcccta ttcggctgga
      841 tgcagcatta catcatggcg ggcgtttcac agcgtactgt gtatggcctg cgcaaagagg
      901 ccgacgcaaa gcttgcgcgt ctcccgctca agtactatga cagtaacact cgcggtgaga
      961 tcctgagccg cctgaccaac gacatcgaca acatcgcaag ttcgttgcag cagagcctga
     1021 accagctcac gacagcggcg ttcacagtca tcggcgtgct catcatgatg ttctccatca
     1081 gccctctgct tgccgcaatc gcattggcca cactgcccct tagcgtccta gttacgctca
     1141 tgatcgccaa gcgctcccag aagcaatttg ccgctcagtg ggagagcacc ggcgctctca
     1201 acagccacat agaggagatg tacaccggcc acaacatagt caaggtattc aacaggcagc
     1261 agacggccgt cgacgcgttc gataaggaaa acgagcgcct gttccaggcg ggcttcaaag
     1321 cacacttcat atccagcacc atccagccgg gtatgcagtt catcaataac ctgaactacg
     1381 tggcggtgtg cgtcatcgga ggcatgaagg tggcaagcgg cgccctttca ctcggcgatg
     1441 tgcaggcctt catccaatac tcaaggcagt ttacgcagcc cattgtccag accgccagca
     1501 tcatgaacat ccttcagtcc acggtggcct cagccgagcg cgtattccaa ctcctcgatg
     1561 agcaggagga ggtccccgac accgccaacc cggtcaagct cgaacatgtg gcagggcatg
     1621 tcgtgttcga caacgtgtcg ttcaagtaca aggctgagac acccctcatc gaacacatgc
     1681 acctggacgt tcagcccggt cagactgtag ccatagtcgg cccaacgggc gcaggcaaga
     1741 caaccctggt caacctgctc atgcgcttct atgagataga cgagggcagc ataaccatcg
     1801 acggcgtaga catccggcgc atgacccgga atagcctgcg aagtctattc ggaatggtgc
     1861 tccaggacac ctggctattt gccgggacaa tccgtgacaa catcgcgtac ggtcgcgaaa
     1921 acgcctccga gcaggagata atggcggcag ccagggcggc ctacgtcgac cacttcgtac
     1981 gcaccctgcc tgacgggtac aacactaagc tagacgacga tgcgtccaac atatcgcagg
     2041 ggcagaagca gttgctgacc attgcccggg ctttcctggc cgatcctgag atactcattc
     2101 tagacgaggc cacaagctcg gtcgacaccc gcaccgaagt gcttatacag gaggctatgt
     2161 cggaactgat gaaagaccgc accagcttcg tcatcgccca ccggctatca acgattcgca
     2221 acgccgatac catcctggtc atggacaacg gcagcatcgt tgagcagggc aaccacaagg
     2281 ctctcatggc caagcgcggg ttctactacg atctctacaa tagccagttt gccggggcgt
     2341 atgacgaggc ggtgtaatca gtcactcacc cgcctcagcg accacgtgag ggtgttcctg
     2401 acggtgcaag tcacgctgcc gtcgggcgat gtaaccgggt catcgtacca cgaatcggag
     2461 ccctggagcg tcggagcgtc ggggtcgaat ggtttccaca ttgaccggaa gtggtcgtaa
     2521 ctactcacaa gtttcgtcgc tgggatggtt gccgcggagc tgacggagga atcgttgata
     2581 tcgaacttgt cggtccactt ggaggttccg tcgcagctct cggacgtatg cttgatggct
     2641 ccgcccatgg atagaccgaa accccagggg cttggctgca cggcccgcgt gccgagcacc
     2701 acagtgtact ccggcccagc gccgcggtgg tcgacacgaa gctcgatatt tgccgacccc
     2761 tggttcgcgt ccgacatcgc gccattgtac tcacacgtgc cggtcagttt gcgtggtcct
     2821 gacctgcatt cggctatgga ttcatggaca ctgtcgcctg cgatgttggc cgctaccatg
     2881 taggcaaaac tgccagaccc aagtccttca tcgttcaggc ggataccatt caccgtgacc
     2941 tcctccgtcc acttgctgcg gcccttgtcg cttacaaacc agccctcgtg gagcctgctt
     3001 gggcctggac gctcggcact ccaggcggca gtgatctccc tttgaagagt aatgcttccc
     3061 acccagttgg tgggcttgtg cctctcaatc tgaacggtcc gccaggcgct gactgctctg
     3121 cgtatttcgt ctccgagggg acggactatg gccatatcga atgggttgag cgaaaatcct
     3181 ttggacaggc ccatgggcac gtctatctcg atagagccct ccgcaatctc gcgtatgccg
     3241 cccaagggct gatccagctt cggatagagg aatatggtgg ccataccgtt ctcattggtc
     3301 ctcttgatcc aggcagggat ggcagtatcg aagcattcca gaggaacctc caggtagcac
     3361 gtgagcgatt tcgggaaccg gaatctcaca gccacgccgg ccagccgatc gcccttcttg
     3421 ggcaggccag agccgagcca cctccgcacg gagggcaagg gaatcctgcc ttccacgtca
     3481 tcctggtatg atacggtaac gtcaatgcgg attcgcctgt cgccccggcg ctcgagcggc
     3541 gactgcctga gatggatcgc ttgaggcgca tccatcggtt catactgaat ctccacatcc
     3601 agtcgagcgc ccatcatcaa cagaaaggtg tgcatcgagt ccagaagatt attgacggct
     3661 cctgtcacct tggctgcagg ccctatgatg cttgcagttt cctggtcgag cgctgtgagc
     3721 cccgcactga tactggtcgg taccaacgtg tctccgagcg acggcacccc gccgccctgc
     3781 atgcctccct gttcggcgtc ggcgggcggc ccgcccgcgg cgcacaggac acacagagtg
     3841 agcgctgcaa tcacgatagc gccggctctg cgcctcatct gcgaccacct ccaagcagcg
     3901 acagatcggg gcgcacagcc cccgcgatat ccatgctgat cagccatacc tgtaccgggt
     3961 cgaggacaag gcggctcata ggtttctcat cgaggcttat ggcaagcccc gccataggcg
     4021 gcgcttcgcc ttcctgttca gcctggcgcc ggcgctcggc gctgtcgatc agctcctcac
     4081 ctgattcgag attgcgctgt gccacgtcgg cagaggcatg cgccccggca agactggccg
     4141 cggccccaat cgccgccctg gcatccacgg ctctctccgg gtcgttttca taatcggccc
     4201 gcgcattctc gagtccctcc gacgccaagc tcgccatcag tcttaggttc gctctagtct
     4261 ctgcctcacc ggtctccgtt acagcaggaa gcttcccagc ctgcagcacc tcgacatgat
     4321 gctgacgcgc ctgtatggat gccatcgcct caggcgtaag cgatgctcct gcctgctgac
     4381 tcgcggcgtg cgtcatcgaa gctaccatgc tcagtatgga ccgaacctcg tcggcggaaa
     4441 gggtgtcctc tcctgccgcc tgagacgcct gcaatatcgg gtccatgaag tcccatatgt
     4501 cgccacccat caagccctcc accatctgct gcatgctctc cttcgatgat aacgtcgact
     4561 caaaggcggc agcgtccagg cccggttcag actgtgcgtc acgtaccgtg gcgcctatcg
     4621 tgccctgggc gatctcttgc gctttggtta cggccaattc ctgcatactg cctgccagcg
     4681 ccctaggcgc accctcgtcc tgttcggcct gtcccaacag gctgaatggg tattcgtcgc
     4741 gatcgcccag cgcatcgatt agcctgatta cgagaccact ccagtcaccc ggaagtccct
     4801 cagcgcgccg gcgcacgtcc ctcaatatgg actcgacatt cgctgaagta caagcctctc
     4861 cgggcagcgc ttcaaacccg atcccggcgt ccttccatga ttgcaccagt tcctcaaggg
     4921 acatgtagct gccggctaca aaggccctcg ccagaatggc aatctccgcc tcgaacagac
     4981 ctgccgcagg ggtttccgca tcgtcccagt caagccccat atcaggccga agaacgggta
     5041 tactgagggc ggtcatgatc tcgcggatca ccgactcagc ctctttcgca gtagtcgccc
     5101 cgtgcagagc gcgggccaga cgtaacgcct cgccctgagg tgttgccgct gttgcggcag
     5161 catgtgaaaa gccgcctgta acgaaaacgc ccaaggcgag tagaactgcg cacgtaagcg
     5221 cgattctttg tgccttctcg gtcgagctat gccaagccgt cgactcgtag gccaaacacg
     5281 ggtcacctcc taatgaatca gtattcgatc agcccgatgg aaccgatcct gatctccaga
     5341 gccactccat tatccaacct gcgtgccgtt ggcaacatgc cggtcggggg ccaacagcac
     5401 cacgtcgccc tgttcgggta ttccccccag cacgagcact tccgacgaaa accccgcgat
     5461 ccgccgcggc ggcatgttca ctactgccgc cacctgcctg cccaccaggg cctctggggt
     5521 atagcgccgc gtgatctggg cgctcgaacg cctgagcccg agttcgccga aatcgatgat
     5581 tagcttgatc gccggcacac gtgcctcagg gaatggcgtt gcctcgacaa ccgtgccgat
     5641 tcggatgtcg agtttctgga aatcgtcaaa cgcggccatc gctgtcagcc ctcacctttc
     5701 accatcacgc gggttctcag tcgcccagcg cggattgtca tgcggatagt cggggaacca
     5761 cggtatgggc ttgagcctgc ttacggccgt ggcctgggca agccacgaac ctacggcctc
     5821 ttccgcgccg gggcctccta aagactgcgt atttgcgaag atgctcagag gaaccgggac
     5881 tgattccgga cgggccaggg cgtgaggcac acgcacgatc gattccaaca cactccgagc
     5941 gtagcccacc tgcatgagcg acaaagcgcc cacgaagcag ctctcctggc ctccattgcc
     6001 tccacaacct ccataacccc cttggccgcc ttcaccccac tggctctcct ttcgactatc
     6061 tcggctttcg ccactcgccg ccgtcgcgac tcggccaatg tagtatacct gcggatcagc
     6121 gcgaagtccc agaacctgag cataggccag agcagcggat agctctagcg aatcatcatg
     6181 gttgatgatg tagatgaagg cgggcagatg ccgccccaga agaaatggcg ccagggaggc
     6241 atatgtctcc cggctcatca caacaatcgg cacagctctg ttgaccatgg ggacagagag
     6301 ctggtgatca gcgacaagac caggtgtacc caattcggcg cggacatcca cccagaggtt
     6361 gtcggcgccc gcctttggag gacgaacccg ctctgtgaag ctgaaaccgg agcccacctg
     6421 tatcctgacg ccgcgcgaaa acgccggcga cgccgcatac tgctcagcga tgtagtccat
     6481 gagaaggtat tcggcggcgt acatcggaaa gtagtgtcct ccagtggtcc atatctgctc
     6541 agccagggac atcgattcgc gaaaccgcag agcattatcc atgaccatga tgatgtccga
     6601 aaccccattc atcagcagcg ccgggcgggg cacgagatcc gggatgttga ttgggtcggc
     6661 gatctgaagc gcatcgatga agtcctgcgg gttcaccaca ttcgctttag ccgctgcgcg
     6721 gaagatgtct agaacatcag tgcgcccgtg ttcgagtatg ccccctactc ctgagccccc
     6781 tacaacaaac acgccgtttg cgaacctcgg ctctaccttg aaggcgagtg aagcgacaat
     6841 gccgccaagg cttactcctg ctatggaaat ggcgctctga tcaatatcgg ggcgcgcagc
     6901 gagccagtcc acagcgcgac gggcatctac gaccgcgcga cgcaaattct ccctcaccgc
     6961 gtcgggcggc ccgatgatga gctgctgccc aggtatgagg ggaacccctt ccgtccgctt
     7021 atgatgatac gggaggctgg gggtcatcac tgcgatgccc cgcttggcgc agaagtcggc
     7081 tatactccag aactggtatt cggagtcgga gaacattcca tgcagaccga caaggcaggg
     7141 aactgcagac gaggccgacc ccgaagccga gcccaaggcc gcgatcggca tcgtttccgt
     7201 ggaccgcggg accatgaacc gcgcatacac aatctcgccc aagctgctgt atctgacttc
     7261 atactcccga taccaaggcg aggaccccaa cagaacctgc gctgccgcaa gaggcgcctc
     7321 cgaatcgtag gaatagaaag cctccacctc gtcgcgcgaa ggcgcctcgg cgccggcggg
     7381 ccggcccgac caggcagcca ggatggcgca gagcaagatc aaaatgcgga ctggcctatt
     7441 cggcgtgagc gtaataaaca atgttgcagg gccccccgat ggacagttgt agtataatac
     7501 cctggctttt gacgaaaaga catgaactcc tcctgaacgg gtagggtcat cggcatcagt
     7561 caatttcgac acaggcagcg cagctcggag cattccgtgc tgcgctgcgt cttcaggggc
     7621 gcactagact gggggcatca gcagtcctac ctcgcggttc cgaggaaaaa caacgcaacg
     7681 gttccgggtc ctgagtgggc acctatgacc gggtcgacgt agttgatctg gacatctttc
     7741 acgttcagcc tctcacgcac gagcctctcg acgtactggg cgtcctcaat gcagtcgccg
     7801 tgggatatga acaccacctg ctccgtcgga tttgcacagg tcttctccat ctcgtctaca
     7861 agcgcctcaa gcgatctcct cctgccccga accttgccga cggcaacaag cttaccttcg
     7921 tcatcgacgt gcagcacggg cttgacgccc aaaactgtgc ccaccaaggc ggttgcggca
     7981 gatatcctcc cgcctctctt caggtgaaac aagtcgtcta ctgtgaacca gtggcatagg
     8041 tgaagtcgat tcgcaatgag ccactcgctc acctcgtcta tggatttgcc ggagcgccgc
     8101 tgttccgcca catggtagac cagcaatccc tcccccatcg acgcggaaag cgaatcaata
     8161 gtgactatct tgcgctctgg gtaggcatcg cgcaagcttt cggccaccat cgtgcccgca
     8221 ttataactgc cgctcagagc cgacgagaag cctatgtaga gtacgtcgtt gccccttttc
     8281 agaatgcctt cgaatatgtc acggcaggtc gacatgtcga tgagcgatgt gtccaccatc
     8341 tctcctttcc tcatcctgtc gtagaactgc ttgtagtccg ttttctctcc ctgcacatag
     8401 caggggaatt cctccccgcc tacgcgaaag ctaagggaca cgatgtgaat gccgtattgc
     8461 tcgatcagct cgtccgtgag atccgcggat gaatctgtca ctatctcata gcccatgttg
     8521 ttcactccct aacgtagtct tgcaccattc gccatctcaa tgcttcctcc tctaatctcg
     8581 gtcaacccag gggcgcatca gcccaggatt actcttgaca cgcggagaca tataggatat
     8641 attcctattt aggaacactt cttggccgga gggatgcgca cacatgaatg cggacgagct
     8701 agagcaacac cttgcacact tccgggaagt gtgcacgaac gaaggactga aactgacgta
     8761 ccaacgcatc gaggtcttcg aggaagttgt cggatcacgg gatcatcctg acgcggagac
     8821 tatcttcaat cgtgtacgcg atagacttcc caccgtctct ctggatacca tctaccggac
     8881 tctgtggatg cttaacgctg tgggtttgat aaccacggtg ggcccgcccc gtgagaaggt
     8941 tcgtttcgat gcggatgtga cgcctcacca tcacttcgtg tgttcgcggt gcggcgcggt
     9001 ctacgacatc aactgccccg agctcggtcc ggtcgaggcg cccaatcata tagaaggcgt
     9061 cgggcaagtg caatcagtcc gtctggaatt gcgcgggcta tgccggcaat gcgcagggca
     9121 catctagccg attcagtcgg cgagcccaac atagcgggtg gcaggcagtc ggnnnnnnnn
     9181 nncacatgaa acagtcaaca cagcacacct agcatcgccg agaggatgtt cacgaaagga
     9241 gaatgcacat atgagcgaaa ccacaaacac cccatgctgc gacgaaatcg tacgcattcc
     9301 gctgatcgga gagaaggctc cgagcttcca ggcagtcacg acacagggca agatctcgtt
     9361 ccccgacgac tataagggca aatgggtcat tctcttcagc cacccggccg acttcactcc
     9421 cgtatgcacc accgagt
//
LOCUS       DAOTAC010000067         9390 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_6246_length_9390_cov_4.165292, whole genome shotgun sequence.
ACCESSION   DAOTAC010000067 DAOTAC010000000
VERSION     DAOTAC010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 9390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9390
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6246_length_9390_cov_4.165292"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /locus_tag="PLM74_04265"
     CDS             <1..1002
                     /locus_tag="PLM74_04265"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HON42333.1"
                     /translation="SGGTDVYAETVELAAYANRAQGLADSLRFAVAADAPGYVYWIET
                     AGAGPRSNVRVIAAPIAAGEAIARHLLEGQESVIFTSATLAVGENFSYIKRDVGLDLA
                     DSGRVSELLVPSPFDFERQALLAVCDSLPAPDTHGWEEALAEALSGVLEASGGRAFVL
                     FTSYKLLGATARRMEEFFRQHKIKLLQQGEAPRHTLLASFRRDVRSVLFGTDSFWEGV
                     DVPGEALSCVVIPRLPFAVPTVPLVEARIEHLRAGGVDPFREYTLPAAVLRLKQGFGR
                     LIRTTADRGAVVVLDSRTARRYYGRIFIAALPGCTTFSGSVTGICARLAQWLERPEH"
     gene            1102..2004
                     /locus_tag="PLM74_04270"
     CDS             1102..2004
                     /locus_tag="PLM74_04270"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HON42334.1"
                     /translation="MLVFYVGAKSRVLIFAVAVLMLVSFAVTAIGSVAEVGLGSAISS
                     AAGVGRNAIAAVTSGSRLVPIYCVARDDRVCSLTVDATWGDDKTVALLDLFERHGLKV
                     TFFLAGHWIKSYPDKVREIASRGHELGNHSWTHPHMSALSRDKIRDELERTQQAIADL
                     VGKRPVVFRPPFGDYSNTVITVAQECGLKTIQWSVDSLDWKDLSAGQIHERVVKRVRP
                     GAIILFHNAGKNSVEALDWIIRDLKTEGYAMLPVSELLLDGETYIDRSTGEQRSVGPR
                     APVPGTGTRETPERRPAVDSPGPL"
     gene            2037..3038
                     /locus_tag="PLM74_04275"
     CDS             2037..3038
                     /locus_tag="PLM74_04275"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HON42335.1"
                     /translation="MIIVTVKRRTVVLAIVAIVLMIVSACVAMSMPRLKRMTTGVRPG
                     VTLSGVAVGGLFADEVRQVVLGLAPRVERMPREAMYHSETGEVVPEEPGVAVDVDRTV
                     IAVMAAQRGANLEITTRQVPPAVTAAMLAPVYRVATSERAVALAFNVAWGEEWLPQIM
                     AVLSKAGVHCSFFITGTWARKFPDIVQALATAGHEIANHGWDHPNPARMSEADLTELI
                     ERNETLLRELTGVRTQLFAPPYGEVNQRVCKVAARLGYTTIMWTVDTIDWQRPAPEVV
                     AERALKRLAGGNIILVHPTEPTAKALPSILTSLSQQGYRAVIVSELLRLGEPAHDRG"
     gene            complement(3073..4410)
                     /locus_tag="PLM74_04280"
     CDS             complement(3073..4410)
                     /locus_tag="PLM74_04280"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HON42336.1"
                     /translation="MQPNTTVTALVVLLYIASSITGMRSSKPGSQVPEPPGVHETVPT
                     PQTQSNAPKDLAGRNAAISDNANVRSGPGTKYSKVGTLAKNTHVRLVGEVNGWYQVSL
                     PRGGYGWIAGSLLQVAPPTGSITQPRHDVVGYYTINYPGDRSSYDVVHAYSDSLTAIA
                     PFSFVVDGSGNVRGDHSKDAMALATSKGLANLALIHNLTGQSFDKGQISALLNSRPAR
                     QRAVSDILEIVKTHGYSGVNIDFENVPPADRSKLTQFMKELRAALAPSGYRVTMSVPA
                     KYKDAPTSAWVGAFDYYELGRVCDQVMIMTYDEHTSGTRPGPIASLPWVEKVIKYAST
                     QMPKRKVLLGVAAYGYDWNTNTNRARAVTYQQAVANAAKHGKKIQWDADAATPHYRYK
                     SGSVTREVWFESSDSLKAKLKLVEKYSLGGIAIWRLGQEDAGYWKLIQKELLK"
     gene            4668..5225
                     /gene="dut"
                     /locus_tag="PLM74_04285"
     CDS             4668..5225
                     /gene="dut"
                     /locus_tag="PLM74_04285"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HON42337.1"
                     /translation="MTSHGGTPQGMTQGTPKANRSVNSYCNSGSVRVEVLRGSTCPRP
                     GMPCYMSEHATGFDLQACLNQDVQLAPGQRALIPTGLRLAIPQGFEGQVRPRSGLAAR
                     HGITVLNSPGTIDSDYRGEICVILINLGSEPYTISHGDRIAQLVIAPTTRAELVEVES
                     LDTTERGEGGFGSTGVSVGEGSENA"
     gene            5218..5418
                     /locus_tag="PLM74_04290"
     CDS             5218..5418
                     /locus_tag="PLM74_04290"
                     /inference="COORDINATES: protein motif:HMM:NF020945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0E family sporulation regulatory
                     protein-aspartic acid phosphatase"
                     /protein_id="HON42338.1"
                     /translation="MHEELNDRIAELKREIEALRGELRHAVLREASSPGKEGTYAIAD
                     RLDRLIVEFMKVRDQVDEAHRD"
     gene            5498..6013
                     /locus_tag="PLM74_04295"
     CDS             5498..6013
                     /locus_tag="PLM74_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42339.1"
                     /translation="MSKIIGTFSSRDSAESCIRELRNSGFDNNRISLVAKGDGAQGQG
                     QGQGQGRGDRGGGATTMGIGSSAATGVTTGGVLGGVAGLLAGLGALTIPGLGPIVAAG
                     PLAATLTGAVGGGLVGGLVDMGIPQERSQYFESKVREGRILAVVDADSDKVDKAARTM
                     RDFGASDVETH"
     gene            6158..6946
                     /gene="dapB"
                     /locus_tag="PLM74_04300"
     CDS             6158..6946
                     /gene="dapB"
                     /locus_tag="PLM74_04300"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HON42340.1"
                     /translation="MDGEIRVLVTGAAGRMGREVVKAVCGADGLVLAGAVDIALVGQD
                     AGTVAGIRPTGVPIGADLEAALRDLRPDVMVDFTIARAAQANIMTAIDHGVRFVVGTS
                     GMGTEAVEAILHRAAVRGAAGAIVPNFALGAVLMMRFARAAAKYFPDCEIIELHHDGK
                     VDFPSGTAKATAHEVASARGDAVGHARGAGTRGVEIEGVPVHSVRLPGLVAHQEVIFG
                     GPGQTLTIRHDSLGRDSFMPGVLMAIRKAVDLDRVVLGLDPLLD"
     gene            7011..7604
                     /locus_tag="PLM74_04305"
     CDS             7011..7604
                     /locus_tag="PLM74_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009330692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HON42341.1"
                     /translation="MSLEGRTIGVALTGSHCTIPEVLPQIEALIERGASVIPILSYSV
                     ANTDTRFGRAQDIREKLEEMTGAEVIDTISRAEPIGPKRLLDCLIIAPCTGNTLSKLA
                     LGITDTPVLMAAKAQLRNGRPLVIGIATNDGLSGSARNIATLLARKNVYFIPYGQDDP
                     KGKPTSLVARMCDTERTVEEALAGKQVQPLLVEKWKD"
     gene            7716..8732
                     /locus_tag="PLM74_04310"
     CDS             7716..8732
                     /locus_tag="PLM74_04310"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006837480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HON42342.1"
                     /translation="MLNVAVVGATGAVGQEMISAIFELGLPVDDLILASSERSAGRVV
                     STPLGDIEVREATPELFKGVDVAFFAAGGSVSLELAPEAARRGALVIDNSSAFRMNPD
                     VPLVVPEVNIEAARNHKGIIANPNCSTIIAIVPVAPIYRRWGMERMIVSTYQAVSGAG
                     VEAMEALTVESRAVLAGGAPEPSVLPFKSARVHHQIAFNLIPHVDSFGPDGFTREEHK
                     MTDETRKILGDPTLRISATTVRVPVYRSHSESINLQTREHAPIEEVRELLSTAPGVKV
                     VDDPENFLYPMPVHTSGTDDIHVGRLREDPSAPNAIWLWVAGDQLRKGAASNAVQIAV
                     ALYK"
     gene            8747..>9390
                     /locus_tag="PLM74_04315"
     CDS             8747..>9390
                     /locus_tag="PLM74_04315"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289936.1"
                     /note="catalyzes the formation of 4-phospho-L-aspartate
                     from L-aspartate and ATP; diaminopimelate sensitive;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HON42343.1"
                     /translation="MGIVVQKFGGTSVANEESRGMLVAKVTEARHDGKDVVVVVSAMG
                     RKPAPYATDTLLDLVGSVCKSAPARERDAIAACGEIISTVVVATALEAAGCPAVSLTG
                     GQAGISTDSNYTDARITKIEPRRILEVLSDGKVAVVAGFQGVTETGDVTTLGRGGSDT
                     TAAALGATLGAEIVEIYTDVEGVMTADPRVVPEARFQDTMSYTDLIEMANQGAKV"
BASE COUNT         1848 a         2665 c         3055 g         1822 t
ORIGIN      
        1 agcggcggca cggacgtcta tgccgaaacc gtcgaacttg cggcctacgc caaccgtgca
       61 caaggactcg ccgattcgct gcggtttgcg gttgcggccg atgccccggg ctatgtgtac
      121 tggatcgaaa ccgcaggcgc aggtcccagg tccaacgtgc gtgtgattgc ggcgccaatc
      181 gccgcaggcg aggccattgc ccgccatctc ttggaggggc aggagtcggt catattcacc
      241 tcagccacgc ttgctgtggg cgagaacttc agttacatca agcgcgatgt cggcctggat
      301 ctggctgact cggggcgtgt gagcgagttg cttgttccgt ctcctttcga tttcgaacgc
      361 caggcgctgc tggccgtgtg cgacagcctg cccgccccgg acactcatgg ctgggaagag
      421 gcgttggccg aagcgctcag cggcgtgctg gaggcctcgg gcgggcgtgc gtttgtgctg
      481 ttcacgtcct acaagctcct tggcgccact gcgcggcgta tggaggaatt cttccgacag
      541 cacaagataa agctgctaca acagggcgaa gctcctcgcc atacgcttct cgccagcttc
      601 cgccgggacg tgcgctcggt cctgttcgga accgacagct tttgggaagg cgtggacgtg
      661 cccggcgagg ccctctcttg cgtggtcatt ccgaggctac catttgccgt acctaccgtg
      721 ccgctggtgg aagcgagaat cgaacacctt cgcgccggcg gggtcgaccc ttttcgcgaa
      781 tacacccttc ctgccgcggt gctcaggctc aaacagggat tcgggcgcct gattcgcact
      841 actgccgacc gcggggcggt tgttgtgctc gattcgcgca cggcccgccg ctactatggc
      901 cggatcttca tcgcggccct gcccggctgc accacgttct cggggagcgt caccggaatc
      961 tgcgctcgtc tggcccagtg gctggagcgg cccgagcact gagagccgtc caagtcgaat
     1021 gatcggcctc gggtccggat tctatacttc atgaacatgc atatgtacta ggggcaatcc
     1081 agcgaaggag gaagcatccc agtgcttgta ttctacgtgg gcgcaaagag cagagtcctt
     1141 atcttcgccg tcgcggtgct gatgctggtc tcgttcgcgg ttaccgccat cgggagcgtc
     1201 gccgaggtcg gcctaggaag cgccatttca agcgccgcgg gcgttgggag aaatgccatc
     1261 gctgcagtca cttcaggcag caggctggtt ccgatctact gcgtggcacg ggacgaccgg
     1321 gtttgctcac tgaccgtcga cgccacctgg ggcgatgaca agaccgtggc gcttctggac
     1381 ctatttgaga ggcacgggct gaaggtcacg ttctttctgg cggggcactg gatcaagtcg
     1441 taccccgaca aggttcgcga gatcgcgtcc cgcggccacg agttgggtaa ccactcctgg
     1501 actcatccgc atatgagcgc gctctcgcgt gacaagatcc gagatgagct tgaacgcacc
     1561 cagcaggcca tagctgatct cgtcgggaag cgtcctgtgg tcttccgacc accattcggc
     1621 gactacagca ataccgtgat caccgtggcc caagagtgcg ggctgaagac cattcagtgg
     1681 agcgtcgact cgctggactg gaaggacctg agtgccgggc agattcatga gagagtagtg
     1741 aaacgggtgc gccccggcgc gataattctg ttccacaatg cagggaagaa ctcggtggaa
     1801 gctctggact ggatcatccg cgatctgaag acagagggat atgcgatgct ccctgtgtcg
     1861 gaactactac ttgatggcga gacctacata gaccgtagca cgggcgagca gcgctctgta
     1921 gggcccaggg cgcccgtacc cggtacgggc acacgcgaga cgccggagcg ccgaccggcg
     1981 gtggactcgc cggggccact ctaggctcgg agcagggagg gaggaaagga gagaacatga
     2041 tcatcgtgac agtcaagcgg cggactgtgg tgctcgcgat agtggccatt gttctcatga
     2101 ttgtgtctgc gtgcgtggcg atgagtatgc ccaggttgaa acggatgacg actggtgtaa
     2161 ggccgggcgt gaccttaagc ggcgtcgcgg tgggcgggct gtttgctgac gaagtgcgac
     2221 aggtcgtgct cggcctagcc ccccgcgtcg agcggatgcc ccgagaggcg atgtaccatt
     2281 ctgagacggg cgaggtggtg ccggaggagc caggtgtggc ggtggatgtg gaccggactg
     2341 tgatagcagt gatggccgcg cagcgagggg ccaaccttga aataacgacc aggcaggtgc
     2401 cgcctgcggt cacagctgcg atgcttgcgc ccgtgtaccg ggtggctact tcggagcgtg
     2461 ccgtggccct ggcgttcaat gtggcctggg gcgaagagtg gcttccccag atcatggccg
     2521 tgctaagcaa ggccggtgtg cactgctcct tcttcatcac gggcacctgg gcacggaagt
     2581 ttcccgatat cgtgcaggct cttgccaccg cagggcatga gatagccaac cacggttggg
     2641 accaccccaa tcccgcacgc atgagcgagg ccgaccttac cgagctgatc gagcggaacg
     2701 agacgctctt gcgcgagctg accggggtgc gcactcagct gtttgctccg ccctatggtg
     2761 aagtgaacca gagggtgtgc aaagtggcgg cgcgcctggg ctacactacg atcatgtgga
     2821 ctgtggacac cattgactgg caaagaccgg cgcctgaagt cgtagccgag cgtgccctaa
     2881 agagattggc cggcggaaac atcatactgg ttcatcccac cgagcccaca gcgaaagccc
     2941 ttccatcgat tttgaccagc ctgtcgcagc agggctatcg cgccgtcatc gtatcagagc
     3001 tgctgcgcct gggagagcct gcccatgaca ggggatgacg caccagaggg atgaccgcgc
     3061 cgtcgggccg tctcacttga gcaactcctt ctggatcagc ttccagtagc ccgcatcttc
     3121 ctggcccagc cgccagattg ctatcccgcc gaggctgtac ttctccacca gcttcagctt
     3181 tgccttgagg ctatcggagc tctcaaacca tacttcacgt gtgacgcttc cggacttata
     3241 gcggtaatga ggagtcgctg cgtccgcgtc ccactgtatc ttcttgccgt gcttggccgc
     3301 attcgccact gcctgctggt atgttacggc tcttgccctg ttggtgttgg tgttccagtc
     3361 gtagccgtag gcggccacac cgagcaggac ctttcgcttt ggcatctgcg tgctagcata
     3421 cttgatcacc ttctccaccc acggcagaga cgctatgggt cccggccggg tgcccgacgt
     3481 gtgctcatca taggtcatga tcatcacctg atcgcacaca cgccccagtt cgtagtagtc
     3541 gaaggcccct acccacgccg aggtaggagc gtccttgtac ttggccggca ccgacatggt
     3601 gaccctgtag cccgacggcg ccagcgcagc gcggagttcc ttcatgaact gagtcagctt
     3661 cgaacggtcg gccgggggca cgttctcgaa gtcgatattg acccctgaat agccgtgtgt
     3721 cttcacgatt tcgaggatgt cgctgaccgc tctctgtcgc gctgggcgcg agttgagcaa
     3781 ggccgatatc tggcccttgt caaaagactg cccggtcagg ttgtgtatga gtgctagatt
     3841 ggccaacccc tttgacgtcg ccagagccat ggcgtcctta ctgtggtcgc cccggacatt
     3901 acctgacccg tcgacaacga aggagaacgg agcgatcgct gtgagcgagt cggagtacgc
     3961 atgaacaacg tcgtatgacg atctgtcgcc cgggtaattt atggtatagt acccgaccac
     4021 atcatgcctg ggctgcgtta tgctgccggt cggcggcgca acctgaagca gcgaaccggc
     4081 tatccatccg tagccgcccc ggggcaatga tacctgatac cacccgttaa cctctcctac
     4141 caggcgcaca tgagtgttct tggcaagagt gccaactttg gagtacttgg tgccggggcc
     4201 tgaacgtacg ttggcattat cggatatggc tgcgttgcgg cctgcaagat ccttaggtgc
     4261 attgctttgt gtttgggggg tgggtacagt ttcgtggaca ccgggaggct caggtacctg
     4321 ggagcccggc ttactggagc gcatgcctgt tatggagctt gcaatgtaca ggagaacaac
     4381 caaagctgtg actgtcgtgt tcggctgcac ctggtaccct cccttccaca gtatcccgga
     4441 tgatgttacg tgtgccgcag cgcagctgag gatggatttc gctgcctctt cacgtttacc
     4501 ttcaagcaaa taggggagtg aatgggtctt attggacaca ctgttaggcg cacgttgtat
     4561 ttcgtgttga tccgcaggac ttcgacgcaa tggggcgaat ggatatcgaa acgtactggc
     4621 cggcttggcc cggcagtgat aggcatggca gagaaggtga acgacagatg acgtcacacg
     4681 gaggtacgcc gcagggcatg acgcaaggca cgccgaaggc caataggtca gtgaacagct
     4741 actgcaattc ggggagcgtg cgggtggagg tactgcgcgg gtcgacttgc ccccgtccgg
     4801 gcatgccgtg ctacatgagc gagcacgcaa ccggtttcga cctgcaggcc tgcttgaacc
     4861 aggatgtcca actcgccccc ggtcagcggg cgctcatacc cacaggtcta cgactcgcga
     4921 tccctcaggg cttcgaaggg caggtaaggc cgcggagcgg gcttgcggcg cgacatggca
     4981 ttaccgtcct gaactcgcca ggcactattg actcggacta caggggcgaa atatgcgtga
     5041 tcctcataaa ccttggctcc gagccgtaca ctatcagcca cggcgacagg attgcacagc
     5101 ttgtgattgc ccccacgacc cgggcggaac tagttgaagt cgaatcgctt gacactaccg
     5161 agcgcggtga gggcggtttc ggcagcacag gcgtgtcagt cggggagggg tccgagaatg
     5221 catgaggagc ttaacgacag gattgctgag cttaagcggg agattgaagc actcaggggc
     5281 gagttgcgac atgcagtgct gcgcgaggca tcgtctcccg gtaaggaagg cacgtatgcc
     5341 attgccgatc ggctcgacag gctcatagtg gagttcatga aggttcggga ccaggtcgac
     5401 gaggcgcacc gcgactagcg aaggcagaat gaactccggc ctcctgggta cactagctgc
     5461 agtgtccaag ctttccagct tctaaggagg tcgagtcatg tcaaagataa tcggtacgtt
     5521 ctcgtcgcgc gattctgctg agtcctgcat tcgcgagctg cgcaactcgg gcttcgacaa
     5581 taaccgcata tccctagtag ctaaggggga tggagcgcaa gggcaagggc aagggcaggg
     5641 gcagggtcgg ggcgataggg gcgggggcgc cacaacaatg ggcataggtt cgagcgcagc
     5701 tacgggtgtc acaaccggag gagtgctcgg cggggttgcc ggattgctgg ccgggctggg
     5761 cgcgcttaca atcccggggc tcgggccgat tgtggctgca gggccactcg cagccaccct
     5821 caccggagct gtgggcggag ggctcgtcgg cggcttggtg gatatgggaa ttcctcagga
     5881 acgaagccag tacttcgaga gcaaggtgcg cgagggccgc atccttgcgg tggttgacgc
     5941 cgattccgac aaggtggaca aggctgcgcg caccatgcgc gattttggtg cctcggacgt
     6001 ggagacccac tgatccacag acagcacaga gcagctcgcc ggcggcgcta cttccgccgg
     6061 cgagccatcg gcggaatgct gggcgaagcc tgccgctggg gcaggctgcc gaaatgaaca
     6121 gcgaagtctg aagaatgcga cggggaggag ctttgagatg gatggcgaga tcagggtgtt
     6181 ggtaaccggg gctgccggta ggatgggccg tgaagtggtg aaggcagtgt gcggcgccga
     6241 cggtcttgtg ctagcgggcg cagtcgatat cgcgctggtc gggcaggacg caggcacggt
     6301 ggccgggatc aggcctaccg gcgttcccat cggtgccgat ctcgaggcag ccttgcgcga
     6361 tttgcggccc gatgtcatgg tggacttcac catagcccgg gctgcgcagg cgaacatcat
     6421 gaccgccatc gatcacggag tccggttcgt cgtcggcaca tccggaatgg ggacggaggc
     6481 agtcgaagcc atactacata gggcggcggt acgcggcgcg gcaggcgcta tagtacccaa
     6541 cttcgccctc ggtgcggtct tgatgatgcg attcgcccgg gcagccgcga agtactttcc
     6601 agattgcgag atcatagagc ttcaccacga cggtaaggtg gacttccctt cgggcactgc
     6661 caaagcgacc gcccatgaag tagcctcggc tcgaggagat gccgttgggc atgcgcgcgg
     6721 ggccggaact cgcggggtcg agatagaggg agtgccagtc cactcggtgc gcctgccggg
     6781 ccttgtggca caccaagagg tgatatttgg cggtcccggg cagacgctca ctatccgaca
     6841 cgactcttta ggcagggact cattcatgcc cggcgtgctc atggctatca gaaaagccgt
     6901 ggatctcgat cgagtcgtgc tgggccttga cccgctgctg gactaatcaa tcgtccatac
     6961 acctccctgt gacgttcgcc atatactgac tgcacgggag gtgtcacatg atgagcctcg
     7021 aagggagaac catcggggtg gctttgaccg gttcgcactg cacgataccg gaggtgcttc
     7081 cccagatcga agcgctgata gaacggggtg cctcagttat tccaatacta tcgtattcgg
     7141 tggctaacac cgatacgcgt ttcggccgcg cccaggacat acgtgagaag ctggaagaga
     7201 tgacaggcgc ggaggtgatt gacaccatct ccagagccga acctatcggc cccaagcgat
     7261 tgctggactg cctgatcata gccccatgca caggcaacac attgtcgaaa ttggcactgg
     7321 gtatcaccga cactccggtg ctgatggccg ccaaggcgca gctcaggaac ggcaggccac
     7381 tggtgatcgg catcgccacc aatgatgggt tgtcggggtc ggcccggaat atcgccactc
     7441 tgcttgcgcg caagaatgta tatttcatcc cgtacgggca ggacgacccg aaagggaagc
     7501 ctacatcgct ggttgcaagg atgtgcgata ctgagaggac ggtagaagag gccctggctg
     7561 ggaagcaggt tcagcccttg ttggttgaaa aatggaaaga ctagtctgcg acgagcggcg
     7621 aagcatgggg aatttccctc agcgagcagg aatacgacca actgcggcca atatacttag
     7681 tttaatttcg tctgacgcag gggagttgag atgtaatgct taatgtagca gtagtgggcg
     7741 caaccggtgc tgtggggcag gagatgatct cggccatatt cgagcttgga ttgccggtgg
     7801 atgacctcat actggcatcg tcggagaggt ctgcaggtcg ggtggtgtcg acgccgctgg
     7861 gcgatatcga ggtgcgcgaa gcaacaccgg agcttttcaa aggtgtggat gtggccttct
     7921 ttgcagccgg gggcagcgtg agcctggaac tcgcgcctga ggcggcgcgc cgcggcgccc
     7981 tggtgatcga caactctagc gccttccgaa tgaaccccga cgtgcccctc gtggttccgg
     8041 aggtcaatat cgaagcagcc cgcaatcaca aggggatcat tgccaacccc aactgctcta
     8101 cgataatcgc catcgtgccg gtggcgccga tctacagacg gtgggggatg gagcggatga
     8161 ttgtctccac gtaccaggcg gtatcgggcg caggcgtcga agccatggag gcgctcacgg
     8221 tcgaaagccg ggcagtgctg gccggcggtg ccccggagcc gtcagtgctg cctttcaagt
     8281 ccgcgagagt acatcatcag atcgcgttca accttatccc gcacgtagac tctttcggcc
     8341 ccgatggttt cacacgcgaa gagcacaaga tgaccgacga gacccggaag atactgggcg
     8401 accccacgct ccgcataagc gcgaccacgg tccgagtgcc cgtctaccgc tcacattcgg
     8461 agtcgatcaa cctgcaaaca cgcgagcacg ctcccatcga agaggtgcgc gagcttctgt
     8521 caaccgctcc gggagtcaag gtcgtggatg atccagagaa cttcctgtat cccatgccag
     8581 tacacacatc gggcactgac gatatccatg tcggccggct acgcgaagat ccttcggcgc
     8641 ctaacgcaat ctggctgtgg gttgccggag atcagctcag aaagggcgcg gcgtccaacg
     8701 cggttcagat tgctgttgca ctctacaagt aggggaggcg gacacggtgg ggatagtggt
     8761 tcagaaattc ggaggtacgt ccgtcgctaa cgaggaatcg cgtgggatgc tggtggcgaa
     8821 ggtaacagag gcacggcacg acggaaagga cgtagtggtc gtcgtatcag ccatgggccg
     8881 caagcccgcg ccgtacgcca ccgacaccct gcttgacctg gtcggatcgg tatgcaagtc
     8941 tgcgcctgcg cgggagcgtg acgcaatcgc tgcctgtgga gagataatat ctacggttgt
     9001 ggttgcgacc gcccttgagg cggcgggatg cccggccgtg tcgctcacgg gcgggcaggc
     9061 cggcatcagt accgatagca actacactga cgcgcgaatc acgaagatcg agcccagacg
     9121 gattctggag gttctatcag atgggaaggt agcggttgtc gcgggtttcc agggagtaac
     9181 tgagacaggc gatgtcacca cattgggacg cggtggcagc gacacgacgg ccgctgcttt
     9241 gggcgcaacg ctgggcgccg agatcgtgga gatatatact gacgtagagg gcgtgatgac
     9301 cgctgatcct cgcgtggttc ccgaggccag attccaggac accatgtcat acaccgacct
     9361 catagagatg gccaaccagg gcgccaaagt
//
LOCUS       DAOTAC010000068         9370 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_6258_length_9370_cov_5.124423, whole genome shotgun sequence.
ACCESSION   DAOTAC010000068 DAOTAC010000000
VERSION     DAOTAC010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 9370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9370
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6258_length_9370_cov_5.124423"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            41..>292
                     /locus_tag="PLM74_04320"
     CDS             41..>292
                     /locus_tag="PLM74_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42344.1"
                     /translation="MIGHSKAEVLESIVKGAAALPPAAVTCACLVLRDHVPCPLWVVF
                     IPSFLAWASIGLAGLDMQSLLGSLALSVALSPLLAMGLVL"
     assembly_gap    293..302
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            748..2460
                     /locus_tag="PLM74_04325"
     CDS             748..2460
                     /locus_tag="PLM74_04325"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HON42345.1"
                     /translation="MPRKYRLILALALTVALAIGAAGVPGAAAVKFEDWGPHVDEIIM
                     PIIKNADARRIAFERGESIVWAGLTKPTDIDQAKSLPYADMKMTLGFHMFYLCFNMRK
                     GPLDAQPLRQAIAHLTDRDNIIRTLFKGYMLPMSSFVPQVSPFYNKNVPFYEFSAKKA
                     EEVLKNAGYKPDGTGKNWIDPKTGKPLPEMKIFTPTYEVAPTSAELGKMIADSCQKIG
                     LPVKPEPMDFNVMLDKLDIGEFDMYCLAWSLSKLPTFLHSFFHSSMDVEAGYNRPGIR
                     NPELDKVLEDLYYATDLKAARTAADKAQLILAREMPYVPLYSRPYIDAFNKTQVTGYV
                     DMNGFGAASYNNPWTLLNIRRVDRFGKAIEGGTIRWALSEEPKNLNPITASSAYEQEV
                     LSKVFDSLTASHPDTLDDMPWMAEKWDIGTWDVEPGKKGTTVTWYVRKGIKWSDGMPF
                     TAEDVKFTIDYLKENECPRYLDATMDIVKTELINKYTVKVYFNSVSYWHFYNAGISML
                     AKHIWKDVKDWKTFQPWLEPHPTIKGYTKLVGTGPFIMKEYRPGEFVRLVKNPNYWRL
                     KPGK"
     gene            2561..3544
                     /locus_tag="PLM74_04330"
     CDS             2561..3544
                     /locus_tag="PLM74_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HON42346.1"
                     /translation="MGFRSYLLRRLGYAVIVLLVILTLNFILFRVMPGDPEKMIIDPN
                     FKQEAKAALRKQYGLDDPPLVQYKNYMLALLRFDLGLSFNTQRPVWDELKERLPNTIT
                     LFLVTFISTAAVGISLGVYSAANRGRFTERFVVGAGLFAHAVPGFFVQLMLLLLFGYY
                     WPILPIHGTMSAPPPDGAFAQFLDRAWHIILPAGSNLVMGFGSWALYTRNTMLEALGQ
                     DYILTARAKGLPKRDVLYHHALRSVLPPIVTLIFMSLPGAVSGAVITESIFSWFGVGR
                     YLLDATLQMDYPAAQGAFYLIALAVIISNLAADIAYGLVDPRIRVGAGGTK"
     gene            3541..5139
                     /locus_tag="PLM74_04335"
     CDS             3541..5139
                     /locus_tag="PLM74_04335"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HON42347.1"
                     /translation="MRQDAKAAQRADVAKAAAAATPRPLTFWEQYRRKSQGMVGLAIV
                     AVYVIVAVFAPWIAPYNPKDDLYLADASAAPVWMSKLSAKFRDCPPTIRLKLGFDEWN
                     QEELGAATLSPFDDGEGSGIQLSYPATEPRESEAEEDFSFSFVGNAHWNPWASSSPEE
                     PSDQVADEEISTESGASAQEVVLSHVMQYNYKSPNTFSAQFDYSIDAPDTAETSISVE
                     LVTPNGEAYEVWDRTLSGSADSESAIIDARDFDLKLKLGLSFFGEPSQVLFVERGDYT
                     IRLRMSSISPDGPVTVKISPVAFGVLGMVHGVLGVDHMGSDIWSQLIYGTRIALAIGL
                     STAFIAVAIGTTVGIVAGYVGGAVDEFLMRVADVLLAIPELPILIILGALFGKSVLNI
                     VILLALLSWMGIARLVRSQTLSLKERQFVEASRASGAPQSYIMLHHILPNVMPLIFAN
                     LVLRIPGAILAEASLSFLGLGDPRVCTWGRILQNAKEFGAFTTMAWWWLVPPGLALTF
                     MSLAFVFIGNSVNEILNPRYRERS"
     gene            5142..6134
                     /locus_tag="PLM74_04340"
     CDS             5142..6134
                     /locus_tag="PLM74_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012580202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON42348.1"
                     /translation="MALLEVRDLKMYYRTLRGHVRAVDSISFDIERGKALGIAGESGC
                     GKSSMATAVLRLLPTNGEYHGGTIMMDGEDILHMPEEEFRQRIRWSKISMVFQGAMNA
                     LNPVHRVGEQIVEAILHHEEMPREKAVERAESLLDLVGINPKRFGEYPHEFSGGMKQR
                     AVIAMSLACHPSLIIADEPTTALDVMVQAQILKAMAELRSELNLSMMLITHDLSVIAQ
                     TCDSIAVMYAGKIVEYGSVYDVYGTPVHPYAAGLVRSFPNIHAERSAVRSLPGFPPNL
                     LAPPSGCRFHPRCPLADEECMKEEPVLREVAKTGHMAACHKAEIVQQGVDIWAQ"
     gene            6122..7165
                     /locus_tag="PLM74_04345"
     CDS             6122..7165
                     /locus_tag="PLM74_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON42349.1"
                     /translation="MGAVAAKLDRKQFQHGDVIMEVRSLTKYFPVRLSFIQSLRTDKP
                     PVLKAVDDVSFDVYKGEVLGIVGESGCGKTTLARTILRLTDATSGSVRLEDVDIMKLN
                     PLEMKNLRRKMQVIFQDPYESMNPRMDVQTIISEPLRIQGLAKTVHEARDVVSSVLAD
                     VEMIPPEEYMSRYPHELSGGQRQRVAVARALALDPDFIVADEPVSMLDVSIRGEVLNL
                     MLNLSREKGVTFVYITHDLATARHICDRIAVMYLGKIVEMGTAEEVIRNPLHPYTTAL
                     IGAVFVPDPTHRGISEVLKGEIPSPVNPPSGCHLHPRCPYAMDICREAEPNFLVYEGT
                     HQAACHRAEKARR"
     gene            7303..8280
                     /locus_tag="PLM74_04350"
     CDS             7303..8280
                     /locus_tag="PLM74_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42350.1"
                     /translation="MSGGELVSVPQDHGLRGMIACITDQVGQPGLMVLDVASGSSAVL
                     ASADCSWRSVPAVAPSGDRVALQGADGRLKVVNVIADQTESMGEPIPGLITPKWSPGG
                     NSIMYELDAATYVVDLDEAHGQSSLVAAMATWMGDGSRIAYVADSVLHARDTWTGQAV
                     QLTEGPERIWGLAASLDGAHAAYVASDDEGALLRVVDIFTREAWDARTSRGIWPWSLT
                     WSPDGKMLAYCVGGSGERGGQRVSVYMVDARGISASSTVEPNIVAGVVSDPQCAGLAW
                     SPDSQAVAIAARTSGSGRYAVHAITRAGETHQLTEQGNCILPDWGPSPI"
     gene            complement(8350..8997)
                     /locus_tag="PLM74_04355"
     CDS             complement(8350..8997)
                     /locus_tag="PLM74_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016328862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS and ACT domain-containing protein"
                     /protein_id="HON42351.1"
                     /translation="MFVRDVMTANPITVTPDVSVMEALEIMKRSKVRRLPVVQGGKLV
                     GIVTDRDLLQVSPSPATSLSVFEINYLISKMTVKEAMTTQVITVSPDDTIEKAAIVMR
                     ENSIGAVPVIDGKMVAGIITETDIFDAFLEMLGATRAGVRLTVDLPDRKGELARVAGA
                     ISEAGANIVSVATFELSPGTVRIVIRLEPENADKAAEAISACGFKVVHRVRSYVQ"
     gene            complement(9176..>9370)
                     /locus_tag="PLM74_04360"
     CDS             complement(9176..>9370)
                     /locus_tag="PLM74_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42352.1"
                     /translation="PGEGALTRCPKCGSPVRRLFSSRMGVIFHGAGYYVTDHRSEEYK
                     KAARADNTGTGSCSSDSCSK"
BASE COUNT         1902 a         2656 c         2844 g         1958 t
ORIGIN      
        1 tggaaggcag cctggctaca gcctgggggg tcagagatcg gtgatcggcc attcgaaagc
       61 ggaagtactg gaatcgattg tgaaaggcgc ggcagcgttg cctccggctg ccgtgacttg
      121 cgcatgtctg gtacttcgcg atcatgtgcc ctgcccgctc tgggtcgtgt tcatcccttc
      181 ttttctggcc tgggcatcca tcggacttgc cggcctcgac atgcaatccc tacttggttc
      241 gctcgccctg tcagttgcgc tctcccctct actcgcaatg ggactcgtgc ttnnnnnnnn
      301 nntccgtcca gcccgtctga agcatcccga gtggcactca ccacgcttcg ccagtcgata
      361 gctgccacaa tcggcatcat ggctcccctc gcgcttgttt cagcgtgcat cggcgggact
      421 atccgggcgt ctcttgacag agcgaagccg cctacttggg tgaagtgatc tgcgccgaag
      481 cagaaggaat tcctgagggc ttctagaata cctaataagc ggcaggattg cgtgagtcgc
      541 ccaaatcgcc tccccggtac ctacatcgga aggaattctg cgtgggtctg ttgaactaac
      601 tcgcatgtgt cctagtgtcc ttactatcta ttccgcgtcg caacgcggca gattgggagg
      661 agggagtgcg aggggtactc atgattctct tggtcctgcc gcgatatcgc tagacgaaaa
      721 acagacgaac aaggaggtat acgtgtaatg ccgaggaagt atcgcttgat tcttgctttg
      781 gcgctcacgg tggccctggc cataggcgcg gccggagttc cgggcgctgc tgccgtgaag
      841 tttgaggact ggggtcctca cgttgacgag atcattatgc ctatcatcaa aaacgcggat
      901 gcccgcagga tcgcctttga acgcggcgag agcatagtct gggctggtct caccaagccc
      961 accgacatcg accaggcgaa gtcgcttccc tacgccgaca tgaagatgac cctgggcttc
     1021 cacatgttct atctctgctt caacatgcgc aagggccccc tggacgctca gccgctccgc
     1081 caggccattg cgcatcttac tgatcgcgac aacatcatcc gcacgctgtt caagggctat
     1141 atgctcccca tgagcagctt cgttccgcag gtctcgccgt tctacaacaa gaacgtgcct
     1201 ttctacgagt tcagcgcgaa gaaggctgaa gaggtcttga agaatgctgg ctataagccg
     1261 gacggcactg ggaagaactg gatcgacccc aagaccggca agcctctccc tgaaatgaag
     1321 atcttcacgc ccacatacga ggttgctcct acatcggccg aactgggcaa gatgattgca
     1381 gactcctgcc agaagatcgg tcttccggtc aagcccgagc ccatggactt caacgtcatg
     1441 ctcgacaagc ttgacatcgg cgagttcgac atgtactgct tggcctggtc gctgagcaag
     1501 ctcccgacgt tcctccacag cttcttccac tcgtcgatgg atgtggaagc cggctacaac
     1561 aggcccggca tccgcaaccc tgagctggac aaggtactgg aggatctgta ttacgccacc
     1621 gacctgaagg cggccagaac cgccgccgac aaggcccagc tgatcctggc tcgcgagatg
     1681 ccctacgtgc cgctatattc caggccgtac atcgatgcgt tcaacaagac gcaggtaacc
     1741 ggctatgtcg acatgaacgg ttttggcgcc gcaagctaca ataacccgtg gactctgctg
     1801 aacatccgca gggttgaccg gttcggcaag gccattgagg gcggcaccat aaggtgggcg
     1861 ctctccgagg aacccaagaa cctgaacccg atcaccgcaa gcagcgctta cgagcaggaa
     1921 gtgctatcta aggtgttcga cagcctgacc gcgtcccatc cggatacgct cgatgatatg
     1981 ccgtggatgg ctgagaagtg ggatatcggc acctgggacg tcgaacccgg caagaagggc
     2041 accactgtca cctggtacgt caggaaggga atcaagtggt ccgatggtat gccattcaca
     2101 gctgaggacg tcaagtttac catcgactac ctgaaggaga acgagtgccc tcgttacctc
     2161 gatgcgacta tggacatcgt caagacggag ctgatcaaca agtatactgt gaaggtctac
     2221 ttcaactccg tcagctactg gcatttctac aacgccggta tcagcatgct cgccaagcac
     2281 atctggaagg atgttaagga ttggaagacg ttccagccct ggctggagcc gcatccgacg
     2341 attaagggct acaccaagct cgtcggcacc ggtccattca tcatgaagga gtaccgtccg
     2401 ggcgagtttg tcaggctcgt caagaacccc aactactggc gcctgaagcc cggcaagtag
     2461 acgcagcagc gcgaccaaac cgaattgcgc ggaccgccgc ccgcgatgca aaatccgggc
     2521 ggcggttccg ggtgaagcgg cagaacgagg tgagatacac ttgggttttc gctcatacct
     2581 tctgaggcgg ctaggatatg ccgttatcgt tctcctcgtc attctcacgc ttaacttcat
     2641 actctttcgt gtgatgccgg gcgatcccga gaagatgatc attgatccca actttaagca
     2701 ggaagccaag gcggcgctga ggaagcagta tggtctggac gatccgcccc tggttcagta
     2761 caagaattac atgctcgcac tgctcaggtt cgacttggga ctctctttta acacgcagcg
     2821 tcctgtgtgg gacgagctca aggagaggtt gcccaacacc atcacgctct ttctggtgac
     2881 gttcatatcc acagccgcag tgggcatatc tctcggggtg tactccgcgg ccaacagggg
     2941 gcggtttacg gagaggttcg tcgttggcgc cggcctgttc gcccacgctg ttccgggatt
     3001 cttcgtccag ctcatgctcc tgcttttgtt tggttactac tggcctatat tgcctataca
     3061 tggcacgatg agcgcgccac cgcccgacgg cgcctttgcc caatttcttg accgtgcgtg
     3121 gcacataatt cttcccgccg gatccaacct tgtcatgggg tttggatcat gggcgcttta
     3181 caccaggaac accatgctag aggccctggg ccaggactac atcctcactg caagggccaa
     3241 agggcttccc aagcgggatg ttctgtacca tcatgctctg cgtagcgtgc tgcctcctat
     3301 cgtgaccctg atcttcatgt ctttgccggg agcggtatcg ggggcggtca tcacggagag
     3361 catcttctcc tggttcggcg tgggtagata cttgctcgat gctaccctcc aaatggacta
     3421 tccggcggca cagggcgctt tctacctgat tgcgctggcc gtcatcatat ctaatctagc
     3481 ggcggatatc gcgtacggcc tggtcgaccc gaggatccgc gtgggggcag gtggtaccaa
     3541 atgaggcagg acgccaaggc agcccagagg gccgacgttg ccaaggccgc agccgcggct
     3601 actcccaggc cgctcacgtt ctgggagcaa taccgcagga agtcgcaggg catggtgggc
     3661 ctggcaatag tggcggttta cgtcatcgtt gcagtgttcg ctccatggat agcgccgtac
     3721 aaccccaagg atgacctcta cctcgcagat gcgagcgccg cgccggtctg gatgtccaag
     3781 ctcagcgcga agttccgaga ttgccctccg acgatccggc tcaagctggg ctttgatgaa
     3841 tggaaccagg aggaactggg cgcagccact ctatcgccgt ttgacgatgg cgaagggagc
     3901 ggaatccagc tcagttaccc tgcaactgaa ccgcgggaat ctgaggcgga agaggacttc
     3961 agtttctcgt tcgtgggcaa tgctcattgg aacccttggg cttcaagcag tcccgaggaa
     4021 ccatccgacc aagtcgcaga tgaagagatc tcaacggaaa gcggtgcttc cgcgcaggaa
     4081 gttgtgctga gccatgtgat gcagtacaac tacaagtcgc ccaacacgtt ttccgcgcag
     4141 ttcgactact cgatagacgc gcccgacacc gccgagactt ctattagtgt cgagctggtg
     4201 actccgaatg gggaagcgta cgaagtgtgg gacaggactc tgtcggggag tgcagactcc
     4261 gagagcgcga taatcgacgc ccgcgacttt gatctcaagc taaaactggg gctgtcgttc
     4321 ttcggcgagc ccagccaggt gctatttgtc gagaggggcg actacacaat ccggcttagg
     4381 atgtcgtcca tctcgcccga cgggccggta accgtgaaga taagtccggt ggcctttgga
     4441 gtcctcggca tggtgcacgg cgtgcttgga gtcgaccata tgggttccga catctggtcg
     4501 cagcttatct acgggacgcg catagcgctc gccataggcc tgtcaacggc tttcatagcg
     4561 gtagccatcg gcaccactgt agggatcgta gcgggctacg ttggcggcgc ggttgatgag
     4621 ttcctgatgc gtgtggcgga cgtgctgctc gccataccgg aactgcccat actgattatc
     4681 cttggcgcgc tattcgggaa gagcgtactc aacatagtga tactactggc gttgctgtca
     4741 tggatgggca tcgcgcggct cgtgcgctcc caaacgctat cgctcaagga gcggcagttt
     4801 gtcgaggctt cccgggcatc gggggcgccg cagagctaca tcatgctgca tcacatactg
     4861 cccaacgtga tgcctctgat cttcgccaac ctcgttctgc ggataccagg cgccatcttg
     4921 gcggaggcgt cactcagctt cctgggccta ggcgacccgc gagtgtgcac atggggccgc
     4981 atactgcaga acgcaaagga gtttggggct ttcaccacga tggcctggtg gtggctggtc
     5041 ccgccggggc ttgcactgac attcatgagc cttgcctttg tgttcatcgg aaactcagtc
     5101 aacgagatcc taaatccacg ctatagggag agatcgtaat catggccctg cttgaagtga
     5161 gagacctgaa aatgtactac cgtaccctcc ggggccatgt cagggcggtc gattccataa
     5221 gcttcgatat cgaacgtgga aaggctctgg gaatcgccgg agagtccggg tgtggaaagt
     5281 ccagcatggc aacggcagtc ctcaggctgc tgccgactaa cggtgagtat cacggcggaa
     5341 cgataatgat ggatggcgag gacattctgc acatgccgga ggaggagttc cggcagagaa
     5401 tccggtggag caagatatct atggtgtttc agggcgcgat gaatgctctc aaccctgttc
     5461 accgcgtagg cgagcagatc gtcgaagcca tcctccacca cgaggagatg ccaagggaga
     5521 aggcagtgga gcgggctgaa agcctgctgg atctggtcgg catcaacccc aagcggttcg
     5581 gcgagtatcc tcatgaattc agcgggggca tgaagcagag ggcggtcatc gccatgtcat
     5641 tggcatgcca tcccagcctg ataattgcgg acgagcctac cactgccctg gacgtgatgg
     5701 tgcaggcgca gatcctgaaa gccatggctg agctcagatc tgagctcaac ttgtcgatga
     5761 tgctcattac tcacgaccta tcggtcattg cgcagacctg tgacagcata gcggtgatgt
     5821 acgccggcaa gatagtcgag tatggaagcg tgtacgatgt gtacgggacg cctgttcatc
     5881 cgtacgcagc cggcctggtc aggtcattcc ccaacattca cgccgaaaga tccgcggtca
     5941 ggtcccttcc gggcttccct ccgaacttgc tggcgcctcc ttccgggtgc aggttccatc
     6001 ctcgatgtcc gctcgcggac gaagagtgca tgaaggagga acccgtattg cgcgaagtcg
     6061 cgaaaaccgg tcatatggcg gcatgccata aggcggagat tgttcagcag ggggtggaca
     6121 tatgggcgca gtagcggcta agcttgatag gaagcagttc cagcatggcg acgtgataat
     6181 ggaggtacgg agcctgacca agtacttccc cgttaggctc agcttcatcc agtcgctgag
     6241 gaccgacaaa cctcccgtgc tgaaggccgt cgatgacgtg agcttcgatg tttacaaagg
     6301 cgaggtgctc ggcatagtgg gcgagtcggg ctgcggcaag actacgctgg cccgcactat
     6361 tctgaggctg accgacgcga catccgggtc ggtcaggctc gaagatgtcg acatcatgaa
     6421 gcttaaccct ctggagatga agaacctgcg ccgcaagatg caggtgattt tccaggaccc
     6481 gtacgagtcg atgaacccca ggatggacgt gcagacgatc atctctgagc ctctgcgcat
     6541 tcaggggctt gccaaaaccg ttcacgaggc gcgcgatgtg gtgagttcgg ttctcgcgga
     6601 cgtggagatg atcccgccgg aggagtacat gtccaggtat ccgcacgagc tgtcgggggg
     6661 ccagcgacag agagtggccg ttgcgcgagc gttggctctc gaccccgatt tcattgtggc
     6721 agatgagccc gtgtcgatgc tggacgtgtc gatccgaggc gaagtcctga acctcatgct
     6781 caacctgtca cgtgagaagg gggtcacctt cgtctatatc acgcacgacc ttgccaccgc
     6841 caggcacatc tgtgatagga tcgctgtgat gtatctgggc aagattgtgg agatgggcac
     6901 ggctgaggag gtcatccgga atcctctgca cccgtacact accgctctca tcggggcggt
     6961 gttcgtgccg gatccgactc acagaggcat ttccgaagtc ctgaagggcg agataccgag
     7021 ccctgtgaat ccgccgtcgg gatgccatct ccatccgcgc tgcccgtacg caatggacat
     7081 atgcagagag gcagaaccca acttcttggt gtatgagggg acgcatcagg cggcgtgtca
     7141 cagggccgag aaggcccgcc ggtaatcatc ggcccacgaa ggggccaaca cttctgtagg
     7201 ttgccaaggg ctgcaggcac tctgagagtg cctgcagcat atattttacg ggcgctactt
     7261 atgggcgttt cgttaggcac ttgcggaagc ggggtgtagt cgatgtccgg aggggagctg
     7321 gtttccgtcc cgcaggacca tggattgcgt gggatgatcg cctgcattac tgaccaggtc
     7381 ggccaacccg ggttgatggt gctggatgtg gcgagcggtt cgtcggcggt gcttgccagc
     7441 gcggactgct cgtggagatc agtgccggcg gttgcgccga gtggagatcg cgtggcgcta
     7501 cagggcgccg acggacggct caaggtagtc aacgtcatag ctgatcagac ggagtcgatg
     7561 ggtgagccca ttccggggtt gatcacgccg aagtggtcgc ctggcggcaa ctcgatcatg
     7621 tacgaactgg acgcagctac ctacgtggtt gatctggatg aagcccacgg gcagtcgagt
     7681 ctcgttgcgg cgatggcgac gtggatgggc gacgggagta gaatcgcata tgttgccgat
     7741 agcgtgctcc atgctcgaga cacctggact gggcaggctg ttcagcttac tgagggcccc
     7801 gagcgaatat gggggttggc agctagcctg gacggcgccc atgcggcata tgtggcctca
     7861 gatgatgagg gggctctgct tcgggtggtg gacatcttca ccagggaggc atgggacgcg
     7921 cggacctctc gagggatatg gccctggtcg ctgacatggt cgcccgatgg caaaatgctc
     7981 gcctactgtg ttggaggctc gggggagcga ggcggccagc gtgtgtctgt gtacatggta
     8041 gatgcgcgcg gcatcagtgc cagcagcacg gtggaaccga acatagtggc cggcgtagtg
     8101 tcggacccac aatgcgccgg gcttgcatgg agcccggact cgcaggctgt ggcgatagcg
     8161 gctcggacta gtggatcagg ccggtacgcg gtacacgcga tcactcgtgc cggcgagacg
     8221 caccagctaa ctgagcaggg aaattgcatt ctcccggact ggggtccatc gcccatatag
     8281 cagtgcagac gcggcacgca aggggcgaga agcgagctga ccctcgcccc cgcaggcgtg
     8341 cgccaccggc tactggacgt acgacctcac ccggtgcact accttaaacc cgcatgctga
     8401 tatggcctcg gcggctttgt cggcgttctc aggctccagc cgaataacga ttctaacagt
     8461 gccgggcgac agctcgaagg tggccacgct gacgatgttc gcgccagctt cgctgatcgc
     8521 gccggctact cgggctagtt cccctttgcg gtcgggcagg tcgacagtga gcctgactcc
     8581 ggctctggtg gcgcccagca tttcgaggaa tgcgtcgaag atgtcggtct cagtgattat
     8641 gcctgcgacc atcttgccat cgatgacggg tactgcgccg atgctgttct cgcgcatcac
     8701 tatggcggcc ttttcgatgg tgtcgtcggg cgatacagtg atcacttggg tggtcatggc
     8761 ctctttcacg gtcatcttcg agatgaggta gttgatttcg aatacggaga gcgatgttgc
     8821 cggcgacggc gatacctgca acaggtccct gtcagtaacg atgcccacaa gcttcccgcc
     8881 ctgcacaaca ggcagccttc ggaccttgct gcgcttcatt atctccaggg cctccattac
     8941 cgacacgtcc ggggttaccg ttatggggtt cgctgtcatt acatctctta caaacatgtg
     9001 tgcgcccttc cggggccttt caccttcctt tcggcaggcg ccggcgcgag accggcgcag
     9061 tttcactggc ctggcgcagt tgttctactt ctgtgggcaa cataccttct taggtgagca
     9121 agaggcggga acccccgcct cacgctgaac ggctatcatc aagaaaacgt cgatcctact
     9181 tcgagcagct atctgacgaa cacgagcctg tgccggtgtt gtcggcgcgc gctgccttct
     9241 tgtactcctc tgatctgtga tcagtcacat agtagccagc cccatggaat atcacaccca
     9301 tcctggagct aaaaaggcgc cggacaggag aaccgcactt ggggcagcgc gtgagcgcgc
     9361 cttcgcctgg
//
LOCUS       DAOTAC010000069         9361 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_6264_length_9361_cov_5.308726, whole genome shotgun sequence.
ACCESSION   DAOTAC010000069 DAOTAC010000000
VERSION     DAOTAC010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 9361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9361
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6264_length_9361_cov_5.308726"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="PLM74_04365"
     CDS             <1..357
                     /locus_tag="PLM74_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014117899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HON42353.1"
                     /translation="RTAYLLHRLAMGNQAPTPADILGVATNGGAWLIGRPDLGSLEEG
                     KAADMFLVKVDTLEWVGADFDPASALATIGLYRPVDYTIVAGKVVVKDGRLVNVDETD
                     VARKARELSRRLCEGR"
     gene            439..1797
                     /locus_tag="PLM74_04370"
     CDS             439..1797
                     /locus_tag="PLM74_04370"
                     /inference="COORDINATES: protein motif:HMM:NF016083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF401 family protein"
                     /protein_id="HON42354.1"
                     /translation="MSYLGLAVGTILILAAVRYKVNVGIAAAMAGLIVASTSGGGLAV
                     LYASLREAVIEPAGLTLLVSIAFITMIGNTMKVSGALDDLTGGIGRVIRDARVVAFLL
                     PSLVGLLSVPGSSVFSAPLVDSVGRSLGMTREQRVTTNVMFRHGWYYIYPIYAPLLLI
                     QQISGVPYSAFLLPGSIATLIVFALTARVVFVTEPLTAEPSLSTGDCSGGLCGCSHDS
                     ITVCARAGVSAGDTTATGSRAVASAHPIYSILLAASPILVVLLCGVGTRINFSIATAL
                     GALTALLAAPSDGPYLKEVARRFREGAVRGIDWQVVGTVAGTVFFGRTIVNSQVLASM
                     AAGTGSSASVLSLFVFGFVLPFSSAFIIGSHLPAVGIAGPLLVPLLSGLRNPAGLVVA
                     LYMAASSGYLLSPAHVCFISTVQYLRADVRSSWRMLWGPGGAGLLLGLLFWAVQVAGN
                     LL"
     gene            1981..3855
                     /locus_tag="PLM74_04375"
     CDS             1981..3855
                     /locus_tag="PLM74_04375"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HON42355.1"
                     /translation="MADIVVVAPYREMADMARRIAGQFGSSISIVDGNLQEGLDAARD
                     ASEHGARVVVSRGGTALLITQASDVCVPVVNIPFISNELVHALRQAVRISRHVGIIRF
                     ANTLPPDIATLSDILDARIVDMPILSESEAAACIADAVEDGIGVIVGGVCGVTHAAAA
                     GLPGVLIESCEDSLHLAFSEAVSVVGKTRVLQRRIAVLRQAIDVASDGIIVVDQDGRA
                     VELNRAAEQLTGVRRRDAVGKHVCGRSAAQAAIGGASGSDVPIALGPSTMSYSVVVDG
                     EEIGAVVVAPRTKEAHTRSADGRKGQSTWSSAARYTFDNIMGCSRALQTAIDTAKSYA
                     SCDYDVLLTGETGTGKELFAQSIHNASARRNGPFVTVNCAALPETLLESELFGYAEGA
                     FTGARRGGKVGLFEQADGGTVFLDEIAVMPLSVQQRFLRVLEDRRIMRIGDDRIVSLD
                     VRVVAATNREISTLVDDGGFLPDLFYRLDVLRLALPPLRDRKEDIPIIAQHLMDTCCA
                     GSGLEPRRFTPAAFAVLSEHEWSGNVRELRNAVQRLIVCGRNLTVSATEVKHALGAHA
                     HPLPVREILRSSVRSHEFEQIARVLEECKGNRTVAAERLGISRVTLWRKLRDHVSPGG
                     "
     gene            4072..5037
                     /locus_tag="PLM74_04380"
     CDS             4072..5037
                     /locus_tag="PLM74_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HON42356.1"
                     /translation="MRLARVLVPVVILSLVALMFGQVVAAAGFPEKPITYMICFDPGG
                     ESDITARLQQKYLEDVLKTKVVITYKVGGGGAVGWSELIRSKPDGYTIAGDNLPHTIL
                     QPMQRGDAGYTTEQLKRVYCFESTPCALVVKKDSPFKTLDDFIAFAKKNPGAVTLGGS
                     GSWTANHLGTIELEKAAGVKLTYIPFTGSGSATPALLGGHVTGLMTYTTMAAQQLDKF
                     RILAVAAPKRSAVFPDAPTFRELGYDIVEGAYRGVSVPPNTPDNIVKILADAFEKVNK
                     NPEFAKKMAELAFDLEFMGPAEYTKFIQTRKAYYTDLLKDMDIKQ"
     gene            5134..6654
                     /locus_tag="PLM74_04385"
     CDS             5134..6654
                     /locus_tag="PLM74_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HON42357.1"
                     /translation="MNTALTGILQILSPGVLLLMLGGTVIGLVAGALPGISATMAVAL
                     LVPFTFSMPAISGLALLGAIYMSAIYGGCFSAILINTPGTPSSIGTCFDGYPMARQGR
                     GQQAIIVATVASVLGGLFGVAALAFLAPPLANVALKFGPPEYFWVAIFGLTIIATLCS
                     KSLIKGIMGGLLGLLISTIGIAPIGGDVRFTFHTPALQGGVELVGTLIGLFCLPEVLS
                     MILDGSRTYETVDVKQSKGVFGTTFRQVLAQPMNLLTSSVIGTVVGIIPGAGGSIANL
                     VAYNEAMRSSKHPEKFGTGIIDGVIATETANNATVGGGLIPLLTLGVPGTPVDAVIYA
                     GLLIHGLRPGAELFTRFADVTYGFIFSLFIATLMMLPVGLLLGTALHKLVSKLSIRIL
                     APSIMFLSIIGSYAIRNNIMDVWVMLICGVIGFMLKMLKVDPAPVVLGLILGPLAEAG
                     LVQGLLMGRASGMGWRLFFTRPVSLLLLAMSVFSLVWPIISAQIRRRRAKEVGTGA"
     gene            6647..7090
                     /locus_tag="PLM74_04390"
     CDS             6647..7090
                     /locus_tag="PLM74_04390"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HON42358.1"
                     /translation="MRNSNVVASILMLVIAAILWPQMKHLTYFGIVFPRAVIVALVAA
                     AVLLLLLTLIRREAGKHDIQAPNLGTVAIVVALMAVWIAIMPWLGFYVTSVAVFGVLM
                     LVIDPSVRNAKSFFVSLAGVAIELALFYVAFSTLLEVPLPRGILF"
     gene            complement(7222..8304)
                     /locus_tag="PLM74_04395"
     CDS             complement(7222..8304)
                     /locus_tag="PLM74_04395"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019119321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HON42359.1"
                     /translation="MDKLDIAVVPGDGVGSEVMAEGLKVLHAVARIHGGLEFRLREFP
                     WGCEHYLKTGRMMDDDGLDVLADHDAILLGAVGAPGIVPDHVSLWGLLLPIRKTFDQY
                     VNLRPIKLLQGVPGRLVGKGPDDIDFVVVRENTEGEYSGVGGRVHIGTPYEVAVQSNI
                     FTRTGTERIMRYAFDLARARNKSKSVVSVTKSNACNYSMVFWDEVFREVARDYPDVET
                     HQVHVDAASMYLITKPEWFDVIVATNLFGDILTDEGAAIQGSIGLAAGANLNPERKYP
                     SMFEPIHGSAPDIAGKGIANPIAMIWSVAMMLDFLGHPELGKLVMQAIECTLSDCKAP
                     RPIDLGGTASTQQVGDAVCERLLALK"
     gene            complement(8327..>9361)
                     /gene="larA"
                     /locus_tag="PLM74_04400"
     CDS             complement(8327..>9361)
                     /gene="larA"
                     /locus_tag="PLM74_04400"
                     /inference="COORDINATES: protein motif:HMM:NF033504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HON42360.1"
                     /translation="NGIILPPILEEIAAAGIAKSDTTLVVATGIHRGVTRDEMAETLG
                     SDIVREYRIVNHDCDDESNLVYMGRLSHGTEFYVNRIVYEAEMLVTTGVIGVHYFAGF
                     SGGRKSIFPGVSGRVSIEHNHSLMTSQSATVAALAGNPVSEEMVEAGRKVDVGFIVNV
                     VVDATGNVCAVVAGDVEQAWLEGVRQCREISVTPIDHLFDVAIASAGGYPKDINVYQA
                     QKALDNAQRAVAPGGTIVLVADCTEGFGEPTFERWMAESSSLDDIFARFAQKFELGGH
                     KAYSLARVLREKEVILVSGLPDSTVRQLFMTPARSIDDALRLVAEKYGAAFSCVVMPN
                     AGSIVPHLEN"
BASE COUNT         1787 a         2606 c         2818 g         2150 t
ORIGIN      
        1 aggacggcat atctccttca caggctggca atgggcaacc aggctcccac tccggcggat
       61 atcctcggtg tcgccacaaa cggcggcgct tggttgatcg ggcgacccga cctgggctca
      121 ttagaggaag ggaaagccgc cgacatgttc cttgttaaag tcgataccct tgagtgggtt
      181 ggagcggatt tcgaccccgc atccgcactg gccactatcg gcctctaccg gcccgtagac
      241 tacaccatcg ttgccggcaa ggtcgtcgtg aaagacggcc ggcttgtcaa tgtcgacgag
      301 accgatgtgg ccaggaaagc aagagagctg tcgaggcggc tatgtgaggg aaggtgagcc
      361 actcagaatg ggtggggtgg cacgcaggca atgcagttag gtggctgacc taggtcagtg
      421 cgaaggagcc tggtggtatt gtcgtatttg ggccttgccg taggaaccat cctcatactc
      481 gccgctgttc gctacaaggt gaacgtcggg attgctgccg ctatggctgg actgatcgta
      541 gcttcgacga gcggcggcgg actcgcagtg ctgtatgcct cactcaggga agcggtgatc
      601 gagcccgcag gcctcacgct gcttgtgtcg attgccttca tcactatgat tggcaacaca
      661 atgaaggtat caggcgctct ggatgacctc acgggaggta tcggtcgggt gatccgggat
      721 gcgcgtgtcg tcgctttctt gttgccttcg ctcgtcggct tgctgagcgt gcccgggtct
      781 tcagtgttct cggcgcctct tgtggattct gtgggtagga gcctgggcat gaccagggaa
      841 cagagagtga ccacgaatgt tatgttccgg cacggctggt attacatcta ccccatctat
      901 gcgcccctgc ttctaattca gcagatctcg ggcgttccct acagcgcttt cctcctcccc
      961 ggttcgattg cgactctgat tgtctttgcg ctgacggcgc gcgtggtgtt cgttacagag
     1021 cccttgactg cagagccaag cctgtcgaca ggcgattgta gtggggggtt gtgcggctgt
     1081 tcacacgaca gcatcaccgt gtgcgcacgt gctggcgtca gcgccggcga cacgactgcg
     1141 actgggtcac gagcagtggc atcggcacat cctatctact cgatcctgct ggcggccagt
     1201 cctatcctgg ttgtgctgct gtgcggtgtg ggaacgagga tcaacttctc tattgcgaca
     1261 gctctgggag cattgacggc cctgctcgcg gcgccgtcag atggaccgta cctgaaagag
     1321 gtagcccgca ggtttcgcga gggagccgtt cggggcattg actggcaggt cgtcggaaca
     1381 gtggcgggca cggtcttctt cggcagaacc atcgtcaact cgcaggttct tgcttcgatg
     1441 gccgccggca caggtagttc ggcgagcgtg ctgtcgctgt tcgtgttcgg ctttgtcctg
     1501 cctttctcat ctgccttcat catcggatct catttgcctg cagttggcat cgcgggcccg
     1561 ctgctagttc cacttctatc tgggctccgc aatcccgccg ggcttgtggt tgccctgtac
     1621 atggccgcat ccagcggcta tctcctgtcg cctgcccatg tctgtttcat ctcgacggtc
     1681 cagtacctaa gagctgatgt caggtcgagc tggcggatgc tatggggacc agggggagcg
     1741 ggtcttctgc tggggttgct cttctgggca gtgcaggtcg ccgggaatct tctgtgaccg
     1801 gttttaccag agggaaacgc ctgtaaccgt tgaatactca ttcaggagtt gctgtacaca
     1861 tcgatctctt gatgcactaa catatactca ttggtccttg ctgtttcgct ctgtcgggtg
     1921 acaaggcgta agggctgtct gccggcgccc tacgactacg atgccgaggg agaaatcgca
     1981 atggctgata ttgtggtggt tgccccctat agggagatgg ccgacatggc caggcgaatc
     2041 gctggccaat tcggctcgtc catttccatt gtggacggca atcttcaaga aggcctggac
     2101 gctgcgcgtg acgcttcgga gcatggagcc cgtgtagtcg tgagcagggg cggaaccgcc
     2161 ctgctgatta cccaggcatc tgatgtatgt gtcccagtcg ttaacatacc tttcatctcc
     2221 aacgagcttg tgcatgctct acgccaggcc gtacggatca gtcgacatgt ggggatcatt
     2281 aggtttgcaa acacgctccc acctgatatt gcgactctca gtgacattct tgacgcccga
     2341 atcgtcgaca tgccgatact gtccgagtct gaggcagccg cctgtatcgc agacgctgtg
     2401 gaagatggca tcggggtaat cgtgggggga gtatgcggtg taacccatgc ggcagccgcg
     2461 ggattgcctg gcgtcctcat cgagtcctgc gaagacagcc ttcacctcgc cttttccgaa
     2521 gccgtcagtg tggtcggaaa gactcgcgtt ctccagcggc gtattgccgt gctccgccag
     2581 gccatcgatg ttgctagcga cgggataatc gtggtggatc aggatggacg agctgtcgag
     2641 cttaaccgcg ccgcggaaca gcttaccggt gtgagacggc gggacgctgt gggcaaacac
     2701 gtgtgcgggc gatctgcggc tcaggcggcc ataggagggg cttccggcag cgatgtgcct
     2761 attgccctgg gccctagcac aatgagttac tctgttgttg tggacggcga ggagattgga
     2821 gccgtggttg tggctcccag aaccaaagaa gcgcatactc ggtcggccga tgggcggaaa
     2881 ggccagtcta catggtcatc tgcggcccgg tacacgttcg ataacataat gggctgcagc
     2941 agagcgcttc aaaccgcgat tgacaccgca aagtcatacg cctcgtgcga ctatgatgtc
     3001 ttgctgacgg gcgagacagg caccggcaag gagctgttcg cccagagtat acacaatgcc
     3061 agcgcccgaa ggaatgggcc gtttgtgacg gtcaactgtg ccgccctgcc tgagacattg
     3121 ctcgaaagcg agctcttcgg gtacgcagaa ggggctttca ctggagcgcg gcgaggaggg
     3181 aaagttggtc ttttcgagca ggcggacggt ggcacggtat tcctcgacga aatcgcagtg
     3241 atgccgctat ctgtccagca gagattcctc cgtgtgctgg aggacaggcg gatcatgcgc
     3301 atcggcgacg accgcattgt atctctggat gtacgtgtag tggctgcgac caatcgtgag
     3361 ataagtaccc tcgttgatga cggcggcttt ttgccggacc tcttttatcg ccttgatgtt
     3421 ttgcgactgg cgttgccccc gcttcgtgac cgcaaggagg atattcccat catagcgcaa
     3481 cacttgatgg acacttgttg cgccgggtca gggctcgaac cgcgacgctt cacgccggct
     3541 gcattcgcag tgctgtccga gcatgaatgg tcgggtaatg tccgagagct ccgaaacgcg
     3601 gtacagaggc tgattgtatg cggcagaaac ctaacggtat ccgccacaga agtgaagcat
     3661 gcattgggcg cccacgccca tcccttgcct gtacgggaga ttcttcgttc ctcagtccgc
     3721 agtcatgagt ttgaacagat agcacgtgtg ttggaggagt gtaagggaaa cagaaccgtc
     3781 gccgcggagc gactcgggat tagcagggtg actctatggc ggaagctcag agaccatgtt
     3841 tcacccgggg gttagcgtct tgtttcattc gcgtaacacg gttgtaacat ggtcaacatg
     3901 tagctgcgcg agattactac gttccggctg ttgccgatgc atttcgccat ccgaggcgga
     3961 ctggcatgcc atttgcattt agttagctgg aagggttgtg tcggcccttg cgggacgacc
     4021 tgtcttggtg tctaggtcag gaactgatta tgaaggaggt aggaaaaccc catgagactg
     4081 gcacgagttc ttgtacccgt cgtgatccta agtctggttg ctctgatgtt tgggcaggtg
     4141 gttgcggccg cgggcttccc tgagaagccg attacctaca tgatctgttt cgatccgggc
     4201 ggggagtccg acataacggc taggcttcag cagaagtatc tggaggatgt cctgaagacc
     4261 aaagtcgtca taacctacaa ggtgggcggc ggcggcgcgg tgggttggtc ggagctaatc
     4321 aggtctaagc ccgatggata cacgatagca ggcgacaatc ttccgcacac tattctccag
     4381 ccgatgcagc gcggtgacgc aggctacaca actgagcagc tgaagagggt atattgcttt
     4441 gagagcactc cctgcgccct tgtggtcaag aaggacagtc ctttcaagac gctggacgat
     4501 ttcattgcat ttgctaagaa aaaccccggc gctgtcactc tgggcggcag cggcagctgg
     4561 accgcgaacc acctgggcac aatcgagctt gagaaggcag cgggagtcaa actgacctac
     4621 atacccttca ctgggtccgg gtcagccacc ccggcgctct tgggcgggca cgtgacaggc
     4681 ctgatgacct ataccacgat ggcagctcag cagctcgaca agttcagaat tctggctgta
     4741 gcagcgccga agcgctcggc tgtgttcccg gatgctccga cgttccgaga actcggctac
     4801 gatattgtgg agggcgccta ccgcggagtg tcggtaccgc cgaacacgcc tgacaatatc
     4861 gtgaagattc ttgcggacgc attcgaaaag gtcaacaaga atcctgagtt cgccaagaag
     4921 atggccgaac tcgccttcga cctggagttc atgggccccg cagagtacac gaagttcatc
     4981 cagaccagaa aagcgtacta caccgatctg ctaaaggata tggatataaa gcaataagac
     5041 agcacctgtg agcaaactcg ccgtcggggc caggacgaca acggcctggc cccgatcacg
     5101 gctataggct acggcgggac tggagggtag agcatgaata cggctctcac agggatactt
     5161 caaatactta gtccaggcgt acttctcctg atgctcggag gaaccgtaat cggtcttgtg
     5221 gcgggggcgc ttccggggat ctcagcgaca atggccgtcg cgctgctggt gccgttcacg
     5281 ttctcaatgc cggccattag cggccttgcg ctgcttggcg ctatctacat gtccgctata
     5341 tacggcgggt gcttctcggc gattctcatc aacacgccgg gtacgccttc gtcgataggt
     5401 acgtgtttcg acggctatcc catggcccgg cagggacgtg gccagcaggc gatcatcgtg
     5461 gcaacagtag cgtctgtcct gggaggcctg ttcggcgttg cggcgttggc ctttttggcg
     5521 cctccccttg ccaacgtggc gctgaagttt ggcccgcctg agtacttctg ggtggccata
     5581 ttcggattga ccatcattgc cacgctgtgc tcgaagtctc ttatcaaggg cataatgggt
     5641 ggactgctcg gtcttcttat cagcactata ggaatagcgc cgataggagg agacgtgagg
     5701 ttcacgttcc acacccctgc tctgcagggc ggggtagagc tagtaggaac cctaataggt
     5761 ctgttctgtc ttcctgaggt gctcagcatg atcctcgacg gatcgcgcac ctatgagacg
     5821 gttgatgtca agcagtccaa gggtgtgttc ggcacaacct tccggcaggt gctggcccag
     5881 ccgatgaacc ttctcacctc aagtgtaatc ggcaccgttg tgggcatcat ccccggcgcc
     5941 ggcggcagca tagccaactt ggtcgcgtat aacgaggcta tgaggtcgtc gaagcatccc
     6001 gagaagtttg gtaccggaat catcgacggc gtgatcgcta cagagactgc aaacaatgcg
     6061 accgtgggcg gcgggctgat tcccctgctt acgctgggag tcccgggcac cccggttgat
     6121 gcggtgatat acgcaggctt gctcattcac gggcttcgcc cgggggcgga gctgtttacc
     6181 cggtttgccg atgtcaccta cgggttcatc ttctccctgt tcatagcaac gctaatgatg
     6241 ctgcccgtgg gtctgttgtt aggaacagcc cttcacaaac tggtgtcgaa gctctccata
     6301 cggatcctgg ctccatccat catgttcctc agcataatcg gatcatatgc catcaggaac
     6361 aacatcatgg acgtatgggt catgctcata tgcggcgtga tagggttcat gctgaagatg
     6421 ctgaaggtcg atccggctcc cgtagtgctc ggcctgatcc tcgggccgct tgcggaggca
     6481 ggtttggtgc agggcctact gatgggccgc gcgtccggca tgggctggcg gctattcttc
     6541 acgcgcccgg tatctttgct gctcctggcg atgtcggtgt tctcgcttgt atggccgatt
     6601 atctcggcgc agatacgtag gcgtcgcgca aaggaggtcg ggacaggtgc gtaacagtaa
     6661 cgtagtggcc tccatactga tgctcgtgat agcggcaata ctgtggccgc agatgaaaca
     6721 cctaacatat ttcggcatcg tcttcccgcg cgctgtgatt gtggcgcttg tggcagcggc
     6781 cgttctgctg ttgttgttga cgttgatccg gcgcgaagcc ggcaagcacg acatacaggc
     6841 cccgaacctt ggaaccgtcg ccattgtagt agcattgatg gctgtatgga tcgcgatcat
     6901 gccctggctg gggttctatg ttaccagtgt ggcggtcttt ggagtgctga tgctggtcat
     6961 cgacccgtcg gtgaggaatg cgaagtcatt cttcgtgtcg cttgcgggcg tcgccatcga
     7021 gctggcgctc ttctacgtag cattcagtac cttgctcgaa gttccgcttc ccagggggat
     7081 tctgttctag ttatcggtcg gacttctcat caatgcagcc gggcgagggg tgaccctgtt
     7141 cgcccggctg cattgtatct gggatccggt atcgtgtggc gcctcgcatg gcgacgcgac
     7201 gcggcaggca ggaggccttg tctacttaag tgcaaggagg cgctcacaca cggcatcgcc
     7261 cacttgttgt gtagatgccg taccgcccag atcgatcggc ctcggcgctt tgcagtcgct
     7321 gagagtgcat tcgatcgctt gcattactag cttgcccagt tcgggatggc cgaggaagtc
     7381 cagcatcatt gctacagacc agatcattgc gattggattg gctatgccct taccggctat
     7441 gtctggagcc gagccgtgta tgggctcgaa catggacggg tacttcctct ctgggttgag
     7501 gttagcgcct gcggccagac cgatgcttcc ctgtatggct gcgccttcgt ctgtgagaat
     7561 gtcgccaaat agattcgtgg cgactatgac gtcgaaccat tccggcttgg tgataaggta
     7621 catggaagct gcgtcaacgt gcacctgatg cgtctctacg tcggggtaat cgcgggccac
     7681 ctcgcgaaag acctcgtccc agaagaccat gctgtagttg catgcgttcg acttggtgac
     7741 tgagaccacg ctcttcgact tgttgcgggc gcgggccagg tcaaatgcgt accgcatgat
     7801 ccgctcagtg cctgttcgtg tgaatatgtt gctctgcaca gctacttcgt agggagtgcc
     7861 gatgtgaacc ctgccgccga cccccgagta ttcgccctcg gtgttctcgc gaacgacgac
     7921 gaagtcgata tcatccggcc ccttgcctac gagcctgcct ggtacgccct gcagaagctt
     7981 gatggggcga aggtttacat actgatcgaa tgtcttgcgg attggcagca ggagacccca
     8041 tagtgacaca tggtcaggca ctattccagg cgctccgact gcgccaagca gtatggcgtc
     8101 atgatctgcc agcacatcca ggccgtcatc atccatcatc ctgcccgtct tcaggtagtg
     8161 ctcgcaaccc cacggaaact cgcgaaggcg gaactcgagg ccgccgtgta tgcgtgcaac
     8221 agcgtgcagt accttcagtc cttcggccat cacttcactg cctacgccat ctccgggcac
     8281 tactgcaatg tccagcttgt ccatattgtc cctcctcagc gttaactcag ttttctaggt
     8341 gaggcacaat gctgccggca ttaggcatta ccacgcaaga aaaggcggcg ccatatttct
     8401 ctgcaacaag tcgtaacgcg tcatctatgg agcgagccgg cgtcatgaac aactggcgta
     8461 ccgtgctgtc gggcagtccg gacaccagga tcacttcctt ctcgcggagt acgcgggcga
     8521 ggctgtaagc cttatgtccc cctagctcaa acttctgggc aaaccgtgca aagatgtcgt
     8581 ccagcgatga cgattcggcc atccatctct caaacgtggg ttcgccgaag ccctccgtgc
     8641 aatcggccac gagcactatc gtacctccgg gtgcgactgc cctctgcgcg ttgtctaggg
     8701 ctttctgcgc ctgatagaca ttgatgtctt tcgggtatcc gcctgcgctg gctattgcta
     8761 cgtcgaacag gtggtcaatg ggcgttacag agatctcgcg gcattggcgc acgccctcaa
     8821 gccatgcctg ttccacatcg cctgccacca cggcgcacac gttcccagtt gcgtcaacta
     8881 caacgttgac tatgaaaccc acatcgacct tgcgcccggc ctcgaccatc tcttcgctca
     8941 ccggattacc ggcgagcgcg gctacggttg cactctgtga agtcatgagc gaatggttgt
     9001 gttcgatgga cactctgccg gatacgccgg ggaatatcga ctttcggcct cctgagaaac
     9061 cggcgaagta gtgcacgcct atgacgcccg tggtgaccag catctccgcc tcgtagacaa
     9121 tcctgttgac gtagaattcc gttccgtggc tgagcctacc catgtagacc aggttggact
     9181 cgtcatcgca atcatggttg acgatccgat actcccgaac gatgtcagaa ccgagggttt
     9241 ccgccatctc gtctcgggtg actcctctgt gtatgcctgt tgccaccacc agagtcgtgt
     9301 cggatttggc tatgcctgcc gcggcaatct cctcaagtat cggtgggagg atgataccgt
     9361 t
//
LOCUS       DAOTAC010000070         9345 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_6271_length_9345_cov_4.935737, whole genome shotgun sequence.
ACCESSION   DAOTAC010000070 DAOTAC010000000
VERSION     DAOTAC010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 9345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9345
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6271_length_9345_cov_4.935737"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            595..1272
                     /locus_tag="PLM74_04405"
     CDS             595..1272
                     /locus_tag="PLM74_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HON42361.1"
                     /translation="MHKIYTGKTKDVFLQDDGNVLLHFKDTVTGVGDRIDSGANEVIG
                     EVAGKGRSSFRLTLRFFELLHESGIPTHFVSAGPDTNTIIARLARSFKLEVICREKAW
                     GSFVRRYGSYVHEGDPLPSLVEFTLKDDERGDPLITEDALVALNIVSGEAVDYMKATA
                     RQATSLIAGHLRERGLELIDIKYEFGEVDGQTMIIDEVSGDSMRVARHGQILLQTELE
                     EALFGKA"
     gene            1326..1796
                     /locus_tag="PLM74_04410"
     CDS             1326..1796
                     /locus_tag="PLM74_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42362.1"
                     /translation="MRRRLVLALLLMALLVASFPGPARATADRFDAGLFIPVIGNIVT
                     AEDVDTDTGQPIDITRAFSSGVSYIALWMNMVGTYEGAPLFIKVTYLDSEDAASDTWD
                     FEMQGSESDVGWVFLEPGEGNVDPATGGWRDGWYRITVLYDYQEMYDLYFYVGL"
     gene            complement(1882..3105)
                     /locus_tag="PLM74_04415"
     CDS             complement(1882..3105)
                     /locus_tag="PLM74_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012036130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HON42363.1"
                     /translation="MRRLTLTKSQARQFILAHQGLWPPRCLAGKDGVLEHIDRVGCIQ
                     FDPLDIVGHNSELVLQARVLGFRRAILQELLYDERQLIDGYDKMMSIYSVQDWPYFGR
                     DRESAARDLANGAEAIRSILPRVRKDIMERGPVSSIDLDYDERVRWPWGPARLARAAL
                     ESMYFCGELVIHHRVHTRRVYDLAIHHLPQDLLSAPDPNVSDEEYHDWRVLRRIGGIG
                     LLWERSGDAWLGIPGVRSRERRESLKRLLAQGKVTEVSVEGIAPTLYMRTSDEDTLHS
                     ALRHEGVAPRAAILAPLDNLLWDRRLVTELFDFDYRWEVYKPAPQRDYGYYVLPILYG
                     DRFAARFEPARDSAGRALVIRNWWWEPEITLSDDMRIALIDCFREFLAYLESDSLTID
                     SQVAERSNLGWLQPN"
     gene            3385..3747
                     /locus_tag="PLM74_04420"
     CDS             3385..3747
                     /locus_tag="PLM74_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42364.1"
                     /translation="MRQRVLKGASILALAWGAWWTFFGIASGIGERIGALGTVMHTLP
                     GLVFLAAAFIARRWNLPGGILLVAVGIGALRFFGYLPSHLLSITALLLGLPPMVSGAI
                     FIEAALRRSGGQLKQYER"
     gene            4569..5477
                     /locus_tag="PLM74_04425"
     CDS             4569..5477
                     /locus_tag="PLM74_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012994234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HON42365.1"
                     /translation="MVRLERLSGRPVLRPLPEHGWERSAVFNCAAIGKDGVFHLIYRA
                     CDLPSHDRFGDYVSRMGHAVSLNGVDFVRHDRPVFEGEGQQEQRGVEDPRIVQVEGET
                     TFHMMYTAYGARFDGDYRISMASSKDLVSWSRRGVVLDEPNKDASLFPSRVNGRYAMF
                     HRRWPHIWMAFSDDLVTWADHTVVMTTVPDSWESERIGIAGPPFRTTDGWVLIYHGVD
                     PSNTYRLGWALLDAVDPTKVVYRQSGPILEPELEWERVGWVPNVVFSCGQVVAGGVVY
                     VYYGAADTSIGVAAADLTDFVPKSAE"
     gene            5543..6580
                     /locus_tag="PLM74_04430"
     CDS             5543..6580
                     /locus_tag="PLM74_04430"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HON42366.1"
                     /translation="MGVTIEDVAKRAGVSVSTVSRVLNRKGRISQQTRQRVFDIAREL
                     EYPVDPVQSASRRRTNTIGIMFNRRLRSLVSDPFYGLVMVGVEESLRQDGYQVFFSTL
                     GERDEDIASIQGFASGRRVDGLILAGCDVEREYIDRIMSLGIPLVLVDNNIVSPKVPC
                     VMTDNTAGAREAVEHLIDLGHDDIAFVSGPLTHSSLFERYQGYRQAMEAHGLPVRQEL
                     LVIYEDVCNFSVDGGFEAATRLMNRSRRPTAIFAANDPLALGVMKGALSLGLTIPRDL
                     SVVGFDDVEAAAHTSPPLTTVRVDKTDLGRHAARLLSELIDGGSDYAPYKIVVCTELI
                     VRGSTGPTQRE"
     gene            6603..7490
                     /locus_tag="PLM74_04435"
     CDS             6603..7490
                     /locus_tag="PLM74_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HON42367.1"
                     /translation="MLGRLAEQYRTSDKAGYVMVLPYILHFLLFTAYPLVFAFVLVFN
                     RWDIVTPMEFIGIRNIQRLLRDATFWTAVINTLRFLASHIPLQIVLSLLIAVILNGPI
                     KLRSFFRGAFFLPFVISGAVVTILWQQLYSTDAGILNLILNRIGMQPVPWLTDPAWAI
                     YSIAIMATWKNMGFYIVLFLAGLQNVPHELYEAASIDGANRSQQFWKITLPLLQPVML
                     LVVILSTIGGFSLFIEPFVMTGGGPAESTLSVVLYLYKQAFQFLRMGYAATIGFALAI
                     MIFVVTFIQRKLLDTDQPA"
     gene            7510..8328
                     /locus_tag="PLM74_04440"
     CDS             7510..8328
                     /locus_tag="PLM74_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HON42368.1"
                     /translation="MRTLTKALGYILLTVAALTFIYPFLWMASASLKPENEIGTLALI
                     SRNFSLRGYSAVFKQIPIGRALFNSGLVSITSTTSVLVFSSMVGYALARLRFLGRDSI
                     FNLVLLTMMIPGQLLLIPMYILVVRLGWTDSYRALIIPAMISNMGIIVFRQFFKSLPQ
                     DLVDAARIDGCSDYRILFQIFWPLSKPVLITVGIITFIGSWNEVLWPLIVIRKWNMMT
                     MPQMVTLFHVGGLANAQVAAEMAAAMMLAFPVIVAYLFFQRYFIESLALSGIKG"
     gene            8415..>9345
                     /locus_tag="PLM74_04445"
     CDS             8415..>9345
                     /locus_tag="PLM74_04445"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HON42369.1"
                     /translation="MNPRSTMGVLLALALVCVLSVSAVGAKAPVTLTFWPSSNPQEIE
                     FAKIIVKAWNDSHPDIQVKMEPLPASRSTEEVLLAAIAAGTTPDICANIYPGAISQYI
                     AGKGLYPHDTLPGFYDFVYARTPKDVVDAFTYKDGHVYQIPWKGNPIMLAYNVKLLRA
                     NGIDTKSLATYSGFLAAAERMVKDLDGDGRPDQWLYAPTVDVTWWQRLFDFYTLYIGA
                     SGGKTLLSAAGKVDFENDAAVQVFTFLQTIFKKGLCPKGAMIGDAFLNGKIAATITGP
                     WSVPYYDQNAPAGFEYDFVPIPVPDGMRGPVITF"
BASE COUNT         1945 a         2610 c         2755 g         2035 t
ORIGIN      
        1 cgatcagaat cttaacccag agtttcatcc agtacttgcc ctccctcttg gtttggtccc
       61 gtctcttacg taatcaccag caccagtccg ctgcaccaga tcctgagggt cgttttccga
      121 gcccctgcag ggggaaatga gcgagaaaca tccatacagc aagtacactg agcatattct
      181 catctattcg cccggtctat cgtccgcagt gaagagagcc atcgctcggc cggcagaacc
      241 ggtgtgttga gatcacctcc cgcgcccttt tctgactgcg ccccgagctt atgggtacag
      301 ccaatgactt cgggcgacgc ctcgacaacg ttcggtccca aagccataca tacgccaacg
      361 ttcgatgttt catttatgcc agcatattca acagaagtac ctcagactcc ttcttcacag
      421 cccgaaaccc agcattcaac ctgcttttcg ccttttcacc aggtaggtcg cggatcggaa
      481 tggttggcaa cattcgggcc ggcggtgtag aattagctat cggaacactg catcaactga
      541 ggatcccgtg ggagcactgt acatagactt cgcgatgcag caggagggct gactatgcac
      601 aaaatataca ccgggaagac gaaggacgtg ttcttacaag acgacggcaa cgtcttgctt
      661 cacttcaaag acacggtaac aggcgtaggc gacaggatcg attccggagc gaacgaggta
      721 ataggcgaag tggccggaaa ggggcgctcc tctttcaggc tgacgttgcg tttcttcgag
      781 cttctgcatg aatccgggat acccacacac tttgtcagcg cagggcccga tacgaacaca
      841 atcatcgcca ggttggcgcg ttcgttcaag cttgaagtga tctgccgcga aaaagcctgg
      901 ggcagcttcg tgcgacgtta tggcagctac gtccatgaag gcgaccctct tccatcgctg
      961 gttgagttta cgctaaaaga tgatgagcgg ggcgaccccc tgattacaga ggatgccctg
     1021 gtcgctctga atatcgtgtc gggagaggcc gtagactaca tgaaagccac tgcgcgccag
     1081 gcgacatcgc tgattgcagg acatctgcgc gagcgagggc tggaactgat cgacatcaag
     1141 tacgaattcg gcgaggtcga cggccagacc atgatcatcg acgaagtatc cggcgacagc
     1201 atgcgggtcg cccgccacgg gcagattctg ctccagaccg agttggaaga ggctcttttc
     1261 ggcaaggcgt agatacccct actggcatag ttgcctcagt tctcaggttg aaaggggaga
     1321 tcttagtgag aaggagactg gttctcgcgc tgctgctgat ggcgttgctc gttgcatcat
     1381 tccccgggcc ggcgcgcgct accgctgatc gattcgatgc ggggctgttc atcccggtca
     1441 tcggaaacat cgtcaccgct gaggacgtag acacggatac aggtcaaccc atcgacatca
     1501 cgcgagcgtt ctcctcagga gtgtcctaca tagcgctatg gatgaatatg gttgggacat
     1561 acgaaggtgc tccgctgttc atcaaggtca cgtacctcga ctccgaggat gctgcgtcgg
     1621 atacgtggga tttcgaaatg caaggctccg aatctgatgt cggctgggtc ttcctcgagc
     1681 ctggcgaagg caatgtcgac ccggctacag gcgggtggcg cgatggctgg tatcgtatca
     1741 ctgtcctgta cgactaccag gaaatgtacg atctgtactt ctacgtgggc ctctaggcgt
     1801 gagatggcat gagacggctt gagatggcat aggactatag gccagttcga cgtgcccccc
     1861 agtggtctgg ggggcacacg cttagttcgg ctgcagccag cccaggttcg agcgttccgc
     1921 tacttgactg tcgattgtga ggctgtcgga ctcaagatac gccaggaatt cccggaagca
     1981 atctatcagg gcaatacgca tgtcgtccga gagggtgatt tccggctccc accaccagtt
     2041 ccggatgacc agggcgcggc ctgcgctatc gcgtgccggc tcgaacctgg ccgcgaatct
     2101 gtcgccatac aggataggga gaacgtagta gccgtagtct cgctgtgggg ccggtttgta
     2161 cacctcccag cggtagtcga agtcgaacaa ctctgttacg agccttcgat cccagaggag
     2221 attgtcaaga ggcgcgagta ttgctgcgcg tggagccaca ccctcatgtc gaagcgcaga
     2281 gtggagagtg tcctcatcgc tcgttctcat gtagagcgtg ggagcaatac cttctacgct
     2341 gacctcagtg accttcccct gtgcgagcag ccgcttaagc gactcccgtc gctccctgga
     2401 tctcacaccc ggtatgccca gccatgcatc gcccgacctt tcccatagca agccgattcc
     2461 accgatgcgc cgcagtacac gccagtcgtg atactcctcg tcgcttacgt ttggatcggg
     2521 cgccgagaga agatcctggg ggaggtggtg gatcgctagg tcgtataccc tgcgtgtatg
     2581 caccctatgg tggataacca gttctccgca gaagtacatg ctctccaatg cagcgcgggc
     2641 cagacgtgca gggccccaag gccagcgcac ccgctcgtcg tagtcaagat cgattgatga
     2701 tacggggcca cgttccatga tgtccttgcg gactcttgga aggatcgatc tgatcgcctc
     2761 ggcgccgttg gccagatcgc gcgcggctga ttcccgatcg cggccgaagt aaggccagtc
     2821 ctggacggag tagatcgaca tcatcttgtc gtaaccatct atgagctgcc gttcgtcata
     2881 cagcagttcc tgcagtattg cacgacgaaa acccaagacg cgtgcctgaa gcacaagctc
     2941 cgaattgtgg cccacgatat ccagcgggtc gaattgaatg cagcctacgc ggtcgatgtg
     3001 ctcaagcaca ccgtccttgc cggccaggca acgcggaggc cacagcccct gatgggccag
     3061 aatgaactgg cgggcctggc tcttcgtgag agtcaacctt cgcatttgcg tctacacatg
     3121 cccttcctgc aacataaccg gacatacgga cagcccaata cccggccgtc acccccagag
     3181 attctatcac gaacatctgt tcggatcaac cgcaaccccg cgtcgtcccc gcgtcgcgac
     3241 gccgtgactc tgcggcgctg cggcagcgac gcggcagtac agtagcgaag cagcagtgca
     3301 gtagcgacac tggttgtagt gaatcgtcat gctgatgcac tatactaagt actgacgaca
     3361 cacgaagtaa ggaggcgggc acagatgcga cagcgcgtgc tgaaaggggc atccatcttg
     3421 gccctggcat ggggcgcatg gtggacattc tttgggattg cgtccggaat cggagagagg
     3481 attggggcct tgggaacggt tatgcatacc cttcccgggc tggtgttcct ggctgccgcg
     3541 ttcattgctc gaaggtggaa cctaccgggg ggcatccttc tagtcgctgt cggcataggc
     3601 gcgctgcggt tcttcggata cttgccgtcg cacctactat cgattactgc gttacttctc
     3661 ggattgcctc caatggtgtc aggggccata ttcatcgagg ccgccttgag gagatcagga
     3721 ggtcagctta agcagtatga gaggtagtgg agtcgtgtcc cgaccgctca tgagaacccg
     3781 ctcgaaagtg gctatgcgtt actaacacta tacccccggg ggtatccgag caggcacgcc
     3841 gtcagttttt gccgatagtt tcgtcgatag acgcagtttt tgagtacatg catttggatc
     3901 ggggcctcca gccggcggat cggtgctgcg aggggcgctc tcggggtcgg tagcgaccta
     3961 gtatgtggca ggcgggcggg aggacatacc accacccacg tagcatcgcc agatacatcc
     4021 aggttgtggg acattctgtc gccttccttg cccggttaca tgccgggacc ggccggatac
     4081 cccccgattt tcgcgcttcg gaggcggtcc ggcggtcccc cgaaatcgat gtactcaaaa
     4141 aatgccggta tctccaaaac tattggtgat aactgacgat atgccagttc cagccgtttt
     4201 ggtccacggt catcatagct agccagctaa aaggcctttt tgcgaggatg accccttctg
     4261 ccgtcactct gttcggcaat atacgctcgc acttccactt cgtgatgcct acatgaacta
     4321 tacagacccc atgcgtcctg cagaaactgg ccggtggcgc ccgccaagcg catgatctcg
     4381 gcttgagccg gggtgagggc cgtgaggtga tctcaatgag ccgatagcgg gatccacagg
     4441 taatgtgtgc gcaaacggat gcgcaagaag gaaaggcggg ctcgatgtag aatacactct
     4501 gcgcaaacca gcgcaagcga tgctgcgcaa ttgcccaccg acaacatagc attgatggag
     4561 gtctggaaat ggtgcgactc gagcgtctct ctggccgccc cgttctacgc cccctgccgg
     4621 aacatggatg ggagcggtcg gcggtgttca actgcgccgc cataggcaaa gacggggtct
     4681 ttcatctgat ctatcgagct tgcgatctgc ccagtcacga caggttcggc gattacgtat
     4741 cgcgcatggg ccacgcggtc agcctcaacg gcgtggattt cgtgcggcat gacaggccgg
     4801 tgttcgaggg cgagggtcag caagagcagc gcggggtgga ggatccccgg attgtacagg
     4861 tagagggcga gactacattc cacatgatgt acacggccta tggcgctcgt ttcgacggcg
     4921 attatcgaat atccatggcg agctccaaag acctcgtgag ctggtcgcgg cgtggcgtgg
     4981 tgctcgacga gcccaacaag gacgcctcgc tctttccttc cagagtgaac gggcggtatg
     5041 ccatgttcca ccgcaggtgg ccgcatatat ggatggcttt ttccgacgac cttgtcactt
     5101 gggcggatca tacggtcgtg atgacgaccg tgccggattc gtgggaatct gagcgtatcg
     5161 gcattgcagg gcctcccttc aggacgaccg acggctgggt gctgatatac cacggtgtgg
     5221 atccatctaa tacgtacagg ctgggctggg ctctcctcga cgcggtggat ccaacgaagg
     5281 tcgtctaccg acagagcggg ccgatacttg agccggaact tgagtgggag cgtgtgggct
     5341 gggtaccgaa tgtggtgttc agctgtgggc aagtggtagc cggcggcgtg gtctacgtct
     5401 actacggcgc ggccgatact tcgatcggag tcgctgctgc agatctcaca gattttgtcc
     5461 caaagagcgc cgaatagtgc aaaactatgc gcaaacacag gagcaaatcg cggtatcgcg
     5521 gaattcgagg tgacgctttg agatgggtgt aacgattgaa gacgtggcga aaagggcagg
     5581 cgtgtcggta tcgactgtat ctcgcgtcct caacaggaag ggacgcatca gccagcagac
     5641 gaggcagcga gttttcgaca ttgccaggga gcttgagtat ccggttgacc cggtccagtc
     5701 agcttccaga cggcggacga acaccatcgg tatcatgttc aacaggcgcc tgcggtcgct
     5761 cgtgtcggac ccgttctatg gccttgtcat ggtgggtgtc gaggagagcc tgcggcaaga
     5821 cgggtaccag gtgttcttct cgaccctggg cgagcgcgat gaggacattg cctcaataca
     5881 agggtttgcc tcggggcggc gcgtagacgg ccttatactt gccgggtgtg atgtggaacg
     5941 cgagtacatc gaccgcatca tgtcgctggg aatccctctt gtgctagtcg acaacaacat
     6001 cgtctctccc aaggtgccgt gtgtgatgac tgacaacacg gctggagccc gggaggcagt
     6061 agagcacctg atagacctgg gtcacgatga catcgcattt gtgtcgggtc ctctcactca
     6121 cagttctctg ttcgaaaggt atcaaggcta caggcaggct atggaggcgc acgggctacc
     6181 ggtgcggcag gaactgttgg tcatctatga agacgtgtgc aacttcagcg tggacggcgg
     6241 gttcgaggct gcgacacgcc tgatgaacag gtcgaggagg cccacagcca tcttcgcagc
     6301 caacgatccg ctggctctcg gagtgatgaa aggcgcgctc agcctagggc tgaccattcc
     6361 ccgagatctg tcggttgtgg gctttgacga tgtggaggcg gcagctcaca ccagtcctcc
     6421 gctgaccact gttcgagtcg acaaaacgga ccttggccgc catgcggcga gactgctttc
     6481 agagctgata gacggcggct ctgactatgc tccatacaag atagtggtct gcactgagct
     6541 gatcgtgcgc ggatcaactg ggccgacaca gcgagagtaa tcctacgccg gaggtgatac
     6601 gtgtgctcgg gcggctcgcg gagcagtaca gaacgagcga caaggcgggg tacgtgatgg
     6661 tgctgcccta catcctccac ttcttgctct ttacggcata cccgctggtt ttcgcatttg
     6721 ttctggtgtt caacagatgg gacatcgtga cccccatgga gttcatcggc atcaggaata
     6781 tccagaggtt gctgcgggac gcgacgttct ggaccgcagt catcaacacg ctcaggtttc
     6841 tggcttctca tatcccattg cagatcgttc tgtctctcct cattgcagtc atcctaaacg
     6901 gcccaatcaa gctgaggtcg tttttccggg gagcgttctt tctgccgttc gtcatatccg
     6961 gtgcagttgt cacgatcctg tggcagcaac tatactctac cgacgccggc atactcaatc
     7021 tgatcctcaa ccgcatcgga atgcagccgg tgccgtggct taccgatcca gcctgggcca
     7081 tctactcaat cgccatcatg gctacatgga agaacatggg cttctacatc gttctgttcc
     7141 tggctgggct gcagaacgtg ccgcacgagc tatacgaggc tgcgagcatt gacggcgcca
     7201 ataggtctca gcagttttgg aagatcacgc ttccgctgct gcagccggtg atgctgctcg
     7261 tggtgatact ctccacaatc ggagggttca gtctcttcat cgagccgttt gtcatgaccg
     7321 ggggcggccc ggctgagagc actctgtcag tggtgcttta cctctacaag caggcattcc
     7381 agtttctcag gatgggctat gcggctacca tcggattcgc gctggccatc atgatattcg
     7441 tggtcacgtt catccagcgc aagctgctcg acactgatca gccggcgtag cgaggcggag
     7501 gtgtgatgaa tgcgtactct gacaaaagcg ctgggctaca tattgctgac cgtcgctgct
     7561 ctcactttca tctatccgtt tctatggatg gcatcagcat cgctcaagcc tgagaatgaa
     7621 atcggaaccc tggcgctcat atctaggaac ttctcactga ggggatacag cgcggtcttc
     7681 aagcagatac ccataggaag agccttgttc aactcggggc tggtgtcgat cacctcaacc
     7741 acatcggtgc tcgtgttcag ctctatggtg ggctatgccc tggcgaggct gaggtttctc
     7801 gggcgcgatt ccatattcaa cctagtgctg ctcacaatga tgatcccggg ccaactgctg
     7861 ctgataccca tgtacattct ggtggtccgg cttgggtgga ccgacagcta tcgcgccttg
     7921 atcatcccgg cgatgataag caacatgggg ataatcgtgt tcaggcagtt cttcaagtcg
     7981 cttcctcaag atctggtcga tgcggcaagg atcgacggct gctccgacta tcggatcctg
     8041 tttcagatat tctggccgct ttcaaagccg gtgttgatca ctgtaggcat tatcacgttc
     8101 atcggttcgt ggaatgaggt gctctggcct ctcatcgtca tcaggaaatg gaacatgatg
     8161 actatgcccc agatggtcac cttgttccac gtgggcgggc ttgcgaacgc gcaggtggcg
     8221 gcagaaatgg ccgctgccat gatgctcgct ttcccggtaa tcgttgcgta cctcttcttc
     8281 cagcgctact tcattgaaag cctagctctg tcgggcatca agggatagac aagatggaga
     8341 aggtgacgcg gcgatgcggc gaactggcga ggttccaggg acacaaaatc aaccggaggg
     8401 atgagagaat gcgaatgaac cctaggtcga cgatgggtgt acttctggct cttgcgctgg
     8461 tatgcgtgtt gtcggttagc gcggttggcg ctaaggcccc tgtcacgctg acattctggc
     8521 cttcgtccaa cccacaggag attgagttcg ccaagatcat cgtgaaggca tggaacgatt
     8581 cccaccccga catccaggtg aagatggaac ctcttccggc atcgaggtcc accgaggagg
     8641 tgctgctcgc agctatagcg gcgggcacga ctcccgacat ctgcgccaat atctatcccg
     8701 gcgcgatatc gcagtatatc gcgggtaagg ggctgtatcc tcacgatacc ctgcccgggt
     8761 tctacgattt cgtgtacgcg cgcactccca aagacgtagt cgacgcattc acctacaagg
     8821 atgggcatgt gtaccagatc ccctggaagg gtaaccccat aatgctcgcg tacaatgtca
     8881 agctgctgcg ggccaacgga atcgatacga agtcgctcgc aacctacagc ggcttcctgg
     8941 cggctgctga aagaatggtg aaagacctgg acggcgatgg gcgccctgac cagtggctct
     9001 atgcgccaac tgtcgatgtc acctggtggc agcggctatt cgacttctat acgctctata
     9061 tcggggccag cggcggcaag accctgctca gcgctgcggg caaggtggac ttcgaaaacg
     9121 acgcggctgt gcaggtgttc acgttcctac agaccatctt caagaagggg ctttgcccta
     9181 agggtgcgat gatcggagac gcattcctaa acggcaagat agccgccacg ataaccggac
     9241 cgtggtcagt cccctactac gatcagaacg cgcccgcggg ctttgaatac gacttcgttc
     9301 cgatccctgt gcctgacggc atgcggggac cggtgatcac cttcg
//
LOCUS       DAOTAC010000071         9180 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_6426_length_9180_cov_4.691178, whole genome shotgun sequence.
ACCESSION   DAOTAC010000071 DAOTAC010000000
VERSION     DAOTAC010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 9180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9180
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6426_length_9180_cov_4.691178"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(191..964)
                     /gene="sigG"
                     /locus_tag="PLM74_04450"
     CDS             complement(191..964)
                     /gene="sigG"
                     /locus_tag="PLM74_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014828615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HON42370.1"
                     /translation="MALNKVEICGVNTSKLPVLSSAKMRELLEAVRDGDEEARSRLIY
                     SNLRLVLSVIQRFTNRGEYVDDLFQVGCIGLIKAIDNFDLGQNVKFSTYAVPMIIGEI
                     RRYLRDNNPIRVSRSLRDIAYRALQVRDRLVADTSREPTVTEIAAALGIPREEVVYAL
                     DAIQEPISLFEPIYHDAGDPIYVMDQVSDDKNLDVNWLEGISIKEAMLRLPARERLIL
                     KLRFFEGRTQMEVADEIGISQAQVSRLEKAALKQMRASM"
     gene            complement(1171..1893)
                     /gene="sigE"
                     /locus_tag="PLM74_04455"
     CDS             complement(1171..1893)
                     /gene="sigE"
                     /locus_tag="PLM74_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006520351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HON42371.1"
                     /translation="MIERAVWIVRLLLIRILGRLATGDDSVCYVGSHETLPAPLTPDE
                     EACLIGRLMGGEEDVRAELVERNLRLVVYIARKFESTGVGVEDLVSIGTIGLIKAVNT
                     FDPSKRIKLATYASRCIENEILMHLRKTNRIRSEVSFDEPLNVDWDGNELLLSDVYGT
                     DDDLIYQAIEEEVDRALLSEALAALTGREKRIMELRFGFTGGSERTQKEVADLLGISQ
                     SYISRLEKRILIKLRHQMARAE"
     gene            complement(1923..2870)
                     /locus_tag="PLM74_04460"
     CDS             complement(1923..2870)
                     /locus_tag="PLM74_04460"
                     /inference="COORDINATES: protein motif:HMM:NF015384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HON42372.1"
                     /translation="MIRLYVDSLLLVAGSAFILDLVTLWAVRQMGGVGAPMAAPIPES
                     RPRLRFRSRLRSRFRLAAGAGLSTAVFVAMLGLAQAGLVDLKSGVSIVAAAAAGGASL
                     ILAFPGISLRRVVTALFYRSLLTALAGGAATAAYSFTAGNRVASFVAAIATVGMAAEA
                     GWGAVHRGIRDGLFVVPVDIDFGADRVSVQALIDTGNRLRDPASGNPVIIVEYDAIQS
                     ALPCEVRAALAQPDDDFMARACAVAGSAWSSRFRAIPYSSVGREKGMMVGFRPDRVQV
                     ADGRRMITTSHAVVCVHTSALCPGGNYRALVNPDVLTAA"
     gene            complement(3056..4129)
                     /gene="ftsZ"
                     /locus_tag="PLM74_04465"
     CDS             complement(3056..4129)
                     /gene="ftsZ"
                     /locus_tag="PLM74_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HON42373.1"
                     /translation="MFELEIEVDHLASIKVVGVGGGGSNAINRMISAGLRGVEFIALN
                     TDAQALRMADAPNKLQIGEKLTKGLGAGANPEIGKKAAEESREQLGQSLEGADMVFIT
                     CGMGGGTGTGAAPIIAEIAKEQGALTVGVVTKPFAFEGRRRMAQADQGIAALKDKVDT
                     LIVIPNDRLLQVVERNTSILEAFRVADDVLRQGVQGISDLIIVPGLINLDFADVRTIM
                     TDAGSALMGIGIASGENRASEAARTAISSPLLEASIEGAKGVLMNITGSSNLGLFEVN
                     EAAEIVSSSADPDALVIFGAVIDESMGDDLKVTVIATGFETAVRAQSTPAGSRLPHAD
                     RVQIKPLAGGDDLDIPPFLRRAR"
     gene            complement(4151..5365)
                     /gene="ftsA"
                     /locus_tag="PLM74_04470"
     CDS             complement(4151..5365)
                     /gene="ftsA"
                     /locus_tag="PLM74_04470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HON42374.1"
                     /translation="MGKRDIVVGLDIGSSKVCAVICEQRDDSSLDVVGMGVSPSAGLR
                     RGVIVDIDSAAAAISDAAARAENMSGCALVSAVASVSGAHAVTTYSKGIVAVSRPDRE
                     IGQDDVERVLDASRVVAIPPDREVIHVLPREFVIDGCRGIRRPVGMSGIRLEVETCII
                     TGSATSIQNVRRAAARVGLEIDQLVLQPIAAAEAVLTESEKDLGVLMIDIGGATTDIA
                     CYLDGTLQHTAVIPVGGSHITNDIALVLRLPVGYAEELKIKYGAARADMVNPDEMCGK
                     DNGISRHKLCQIIEARVYEIMEMVKAEADANGLAGQLPAGIVVTGGAAIMRGVVEVAQ
                     DVLKLPSRLGVPTGVSGMTDVVASPAYATALGLVKYVWASSGSASGSTTKRRSSGFAA
                     NVREWFREIFSS"
     gene            complement(5454..6203)
                     /locus_tag="PLM74_04475"
     CDS             complement(5454..6203)
                     /locus_tag="PLM74_04475"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HON42375.1"
                     /translation="MPDYSHMSRAPERQRRIPFPAIALLFIVLSIAAFLLSPYFEVRQ
                     VRVSGAHYLLEREVLLIADIPAKSNVFLVPAKEIERRLTATPRIRKARVDRIIPDTLR
                     IHLEERTTVAYLPYGGYFIDIDEYGCAVAVSEAITDPDVPLLLGVIPTYVAVGERVKP
                     ERSVAVGAAIGAALVRQKIPNLSEVDVTSPDDVVIRTSDGIRVLAGSADGIEERVRLL
                     DSILASLREQDMPVDYIDVRVEAKPVIRPKR"
     gene            complement(6265..7548)
                     /gene="murA"
                     /locus_tag="PLM74_04480"
     CDS             complement(6265..7548)
                     /gene="murA"
                     /locus_tag="PLM74_04480"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HON42376.1"
                     /translation="MEWLRIEGGRELRGRVSVPGAKNSCLKLMALCIMASSECTITNV
                     PSITDVDTMCDLLRAVGAVIVSRRDSVLSIDPRPISCTRLPEQLVRCMRASVQVLGPL
                     LARFGEVQMPYPGGCPIGSRPIDLHLKGLTAMGAQFSEEHGLISGRADRLVGTEIHLD
                     FPSVGATENLMAAACMATGTTVIRNAAREPEIVDQQNFLTRLGAQIRGAGTDTIRITG
                     LPELGSTTYPVMPDRIQAGTYMIAATATRGDVTVTDVVPEHLEAVGAKLAEAGAAIIA
                     SEDSVRVVMGDRPAPISVKSMPYPGFPTDLQAPMAALLTVGRGTSVVTENIFNNRFRY
                     VDELNRMGAQISVDGRTAIIRGVSELTGARVSAPDLRAGAALVVAGLTARGETIIDGV
                     EHITRGYDSLSERLASCGAHISHTVDAPEAASFDS"
     gene            complement(7592..8509)
                     /gene="murB"
                     /locus_tag="PLM74_04485"
     CDS             complement(7592..8509)
                     /gene="murB"
                     /locus_tag="PLM74_04485"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HON42377.1"
                     /translation="MTSMVTAEQARRLRMVYDAKIMLDELMKRYTSFQIGGPADVLVE
                     PHDVEALQTVLQWAWSEGLPVFVLGNGTNLLVADEGIRGVVVRIGPGLDDISFEGECV
                     RAGAGVTLPVLARECAERGLAGLEWAVGIPGSLGGALTMNAGAYSHTVGDVTREVKMI
                     GLDGAVSVKTGEEMQFAQRSSRLSQGDVIAYQALLALRHADPDEIRARTAGYMKDRRI
                     KQPLHMPSAGCVFQNPSGRGAGRYIDAAGLKGLRVGGAEVSPVHANFIVNTGGATARD
                     VLILMGVIRRTVYEKFGVDLVPEVRVVGG"
     gene            complement(8514..>9180)
                     /locus_tag="PLM74_04490"
     CDS             complement(8514..>9180)
                     /locus_tag="PLM74_04490"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HON42378.1"
                     /translation="LGFGSRFVASHHGRPMGQVELSVPGMHNMSNAIAAIALADQLGI
                     DFDVVRTGLARFSGADRRCQVLARTPDVTLVDDYAHHPAEIRATLAALRKAASGRLVA
                     VFQPQRFSRTKLLHDEFVRSFSLADVIAVTEIYYEGTGEEPIPGVTGRHLAESIEAYE
                     GRPVVYLADRDALEDFLRSVMTPGDTIVTMGAGDIYRTSRRFAEELLRSGARPGPSED
                     GRW"
BASE COUNT         1809 a         3008 c         2509 g         1854 t
ORIGIN      
        1 tcatcccccg gcgcgacgac atgcagtcgg atgaggttcc ctgagttgta ggcgggcgtt
       61 gcttcgtcgg aggctctggc tccggatggg cgggcagtcg cggccacgaa ggctatgaag
      121 gccagcgcca gtatggcggc acacgtgact accctaacag cgatttcgtg tttgatggcg
      181 taaccctcct ctacatcgaa gccctcatct gcttgagagc ggccttctcg agcctggaca
      241 cttgtgcctg cgaaatcccg atctcatcgg ccacctccat ctgagtcctg ccctcgaaga
      301 accggagctt caatatcagg cgctcgcgcg cgggcagtct cagcatggcc tcctttatgg
      361 agatgccctc aagccagtta acgtcgaggt tcttgtcatc cgacacctgg tccatgacgt
      421 agatgggatc gcccgcatcg tggtatatcg gctcgaacag tgatatcggt tcctgtatgg
      481 cgtccagggc atacactacc tcctcccgcg ggatgcccag agcagcggcg atctccgtca
      541 ctgtcggttc ccgggaggta tcggcgacaa gcctgtcacg gacctgtaga gcccggtagg
      601 cgatgtcgcg caaggaccgc gacaccctga tggggttatt gtcccgcaaa taccttctga
      661 tctcgccgat tatcatgggc actgcatatg tggagaactt gacgttctgt cctaagtcga
      721 agttgtcgat ggccttgatc aggccgatgc agcccacctg gaatagatcg tcaacgtatt
      781 cgcccctatt ggtgaagcgc tgaatgaccg atagaaccag tcggagattg ctgtatatta
      841 gcctcgagcg tgcttcctca tcgccgtcgc gcacggcctc taaaagttcc cgcatcttcg
      901 cgctcgacag caccggaagt ttcgacgtgt tgacgccgca gatctcaacc ttgttcaagg
      961 ccatttcgga acaactccac tatctgaact tcgtatcgac ggtcttccag taagagtatt
     1021 gctccgatgt tccaggttta ttctgtctca cttgttcgaa ttgggcgtca acatgtcggt
     1081 cgtcaacata atagagcgcc cggtcgtgtg ggtatccccc tcgaccgggc gcgattttgg
     1141 cgtgtgcctt ccactgcccc agtggcggtc ttactcagcc ctggccatct gatgcctcaa
     1201 ctttatcagg attctcttct ccagcctaga tatgtatgat tgcgagattc ccagcaggtc
     1261 ggctacttcc ttctgagtcc tctcggaccc gcctgtgaag ccgaacctca gctccataat
     1321 gcgtttctcc ctgccggtca gggcggccag agcctcgctg agcagggcac gatccacttc
     1381 ctcctcgatg gcctggtata tcaggtcgtc gtcggtcccg taaacgtctg aaaggagaag
     1441 ctcgttgccg tcccagtcga cgttgagcgg ctcgtcgaac gacacttcgc tgcgaatcct
     1501 attggtcttg cgcaggtgca tcaggatttc gttttcgatg cacctggacg cgtacgtagc
     1561 cagctttatc cgcttgctag gatcgaaggt gttgaccgcc tttatcaggc cgatagtgcc
     1621 aatggacacg agatcctcta cgcccactcc ggtgctttcg aacttgcggg cgatgtacac
     1681 gaccaacctc agattgcgct ccaccagttc tgccctgaca tcctcctccc cgcccataag
     1741 ccggccgatc aggcatgcct cctcgtccgg ggtaagaggg gccggaagcg tctcgtggct
     1801 tccgacatag cagactgagt cgtcacccgt agccagtctg ccgaggatcc tgatgagcag
     1861 caatcggaca atccagacgg ctctctcaat catctccatc tgagcaagcc tcccatgtgg
     1921 actcacgcgg cggttaggac atcggggttg acgagcgccc ggtaattacc gcccgggcat
     1981 agtgccgagg tgtgaacgca taccacagcg tgcgaagtgg tgatcattcg cctgccgtcc
     2041 gccacctgga cccggtcggg cctaaagccc accatcatcc ctttctctct gcctacggag
     2101 gagtacggaa tggccctgaa ccgcgacgac caggccgaac ctgcaacagc gcacgcccgc
     2161 gccatgaagt cgtcgtcggg ctgggctaag gctgccctga cctcgcaagg caacgcagac
     2221 tggatcgcat catactccac tattatcacg ggattgccgg aagccgggtc gcgcaagcga
     2281 ttgccagtgt cgatcagtgc ctgaaccgac acacggtcgg cgccgaaatc tatgtcgaca
     2341 ggcactacga acagcccgtc gcgaatgcct cgatggactg cgccccagcc agcctcggct
     2401 gccattccca ctgttgcgat ggctgcgacg aacgaggcta cccggttccc cgctgtgaag
     2461 gagtaggccg ctgtcgccgc gccgccggcc agcgcagtca agagagagcg gtagaacagc
     2521 gctgtaacta cgcgtcgcaa cgatattccg gggaacgcca ggatcagtga tgcgccgcct
     2581 gcagccgccg cggccacgat cgacacgccc gacttcaggt cgacaagtcc cgcctgcgcc
     2641 aggcccagca tggccacgaa cacagccgtc gacaggccgg ccccggccgc cagcctgaac
     2701 ctggacctga gcctggacct gaacctaagc ctgggcctcg actctggtat gggtgcggcc
     2761 atgggcgcac ccacgccgcc catctgccgg acggcccata aggtgacgag gtcgaggatg
     2821 aaagcgctac cggccacgag tagcagggag tcaacgtaca agcgaatcac ctatggcacc
     2881 cccccactac gtaattgtat tcagtgtatc accccctcac tccgaaaggt gtcgggttgg
     2941 ggcgacgaag agcgcgatgg attccgagat gaagaaacgg cgcatcctac ctgcctgaga
     3001 atggatgcgc cgctgtatgt tgccactagg cgactgttat gtacttggac cgctcctacc
     3061 tggcccgcct caggaaaggc gggatatcca gatcatcgcc gccggcgagc ggcttgatct
     3121 gaactctgtc agcgtgagga agcctggaac ccgcaggcgt tgactgggcg cgcacagccg
     3181 tttcgaatcc cgtggcgatc acggtgactt tgagatcgtc gcccattgat tcatcgataa
     3241 ccgcgccgaa gatcacgagc gcatcggggt cggcagaact cgacactatc tcggccgctt
     3301 cgttcacttc gaacaatccc aggttggacg agcccgtgat gttcatcaga acgcccttag
     3361 ccccttcgat cgaagcctcc agcagagggc tggatatggc ggtacgggcc gcttcggagg
     3421 cgcggttttc gcccgaggcg atgccgatgc ccatgagagc ggaacctgcg tctgtcataa
     3481 tcgttctgac gtcggcaaaa tccaggttga tcaggccagg cacgattatc aggtcggaaa
     3541 tgccctgaac gccctggcgg agcacgtcat ctgccacgcg gaacgcttct aggatcgaag
     3601 tgttgcgctc cactacctgc agcaggcgat cgtttggaat gactattagg gtgtcaactt
     3661 tgtctttgag cgcggctatt ccctggtcgg cctgggccat ccggcgtctg ccctcaaaag
     3721 cgaacggctt cgtgaccacg cccactgtga gagctccctg ctcctttgcg atctccgcaa
     3781 taatgggcgc ggcccccgtg ccggtgcccc cacccatacc gcaggtgata aagaccatgt
     3841 cggcgccttc aagcgactgc cccagttgct cacggctctc ctcggcggcc ttcttgccga
     3901 tctcgggatt cgcgccggcg ccaagcccct tagtgagctt ctcgccgatc tgcaacttgt
     3961 ttggagcgtc cgccatgcgc aaagcctggg catccgtgtt caaagcgatg aactcgaccc
     4021 cacgaagccc ggcgctgatc atcctgttga tggcgttgct gccgccaccg cctacaccca
     4081 ctaccttgat gctggccaaa tgatcgacct cgatctctag ttcaaacacc tcgcatcccc
     4141 cttgcagctc tcacgaggag aaaatctccc gaaaccactc gcgaacgttc gcggcaaaac
     4201 cactagatcg gcgcttagta gttgaccctg acgccgatcc tgaactcgcc cacacatact
     4261 tcaccaggcc gagcgccgtg gcgtatgcag ggctcgccac aacatcagtc atgcccgata
     4321 cccctgtggg aacgcccagg cgcgacggca gcttgagcac atcctgggca acctcaacca
     4381 cgccgcgcat gatcgcagcg ccgccggtca ctacgattcc ggcggggagt tgcccggcaa
     4441 ggccattcgc atctgcctcg gccttgacca tctccatgat ctcgtacacc cgcgcctcga
     4501 ttatctggca tagtttgtgc cgggatattc cgttatcctt cccgcacatc tcgtccgggt
     4561 tcaccatgtc cgcacgggcc gcgccgtact tgatcttgag ttcctcagca tagcccacag
     4621 gcagacgaag caccaacgcg atatcgttcg tgatatggct cccgcctacc ggtatcactg
     4681 cggtatgctg gagtgtgccg tcaaggtagc acgcgatatc agtcgtggcg ccgccgatat
     4741 cgatcatcag cacgcccaga tccttctccg actcagtgag cacggcttcg gccgctgcga
     4801 taggttgcag cactagctga tctatctcca ggcccacgcg ggccgccgcg cggcgcacgt
     4861 tctgaatgga cgtggctgaa ccggtgatta tgcacgtctc cacttcaagc cggatgcccg
     4921 acattcctac cgggcggcgg atgccccggc aaccatctat cacgaactcc cggggaagca
     4981 catgaatcac ttcgcggtcg ggcggaatcg ccacgaccct tgatgcatcg agcaccctct
     5041 ccacgtcgtc ctggccgatc tccctatcgg gcctgctaac agccacgatg cccttgctat
     5101 atgtagtcac ggcatgcgcc cctgacacgc tcgccaccgc cgaaaccaag gcacaacctg
     5161 acatgttctc agcccgcgcg gcggcgtcgg atatggccgc cgccgcagaa tcgatatcca
     5221 caatcacccc gcgcctgagg cctgcggatg ggctgactcc catgcccacc acgtccaggg
     5281 atgaatcatc gcgttgctcg catatgaccg cacacacttt gctcgatccg atgtcgagcc
     5341 caactacgat gtctctttta cccaaactag ctccctccgc ccgcaccgaa ccgcgtcgcc
     5401 acgtctttgc atgattctag aactccgtcc gtttccctct tgtgcccgcc ggtttatctc
     5461 ttcggtcgta tcacgggttt ggcctccact cggacgtcaa tgtaatccac aggcatgtcc
     5521 tgttctctga gtgaggccaa gattgaatcg agaagcctta cccgctcctc gatgccgtcc
     5581 gcggagcccg ccagtacgcg gataccgtct gaagtcctaa tcacaacatc atcaggcgaa
     5641 gtcacgtcca cttccgacag gttgggtatc ttttgtcgta ctagggcagc cccgatcgct
     5701 gccccgacgg caacagatcg ctcaggcttg acgcgctcgc ccacggccac gtacgtggga
     5761 atcaccccga gcaatagagg tacatcgggg tcggtgatcg cctcggacac agccaccgcg
     5821 cagccgtact cgtcgatgtc gatgaagtag cctccgtatg gaagataagc cactgtagtg
     5881 cgctcctcca ggtggatccg gagcgtatcg gggatgatcc tgtctacgcg tgctttccgt
     5941 attcgcggcg tggcggtaag tcttctctca atctccttcg caggaacaag aaagacgttg
     6001 gactttgctg ggatatccgc gatcaacagg acctcccgct ctagcaggta gtgggcgccg
     6061 ctgacgcgca cctgcctgac ctcaaaatac ggagaaagca ggaaggcggc gatgctcaga
     6121 acgatgaaca gcagcgcgat cgcggggaat ggtatccgcc gctgccgctc cggcgctcga
     6181 gacatgtggg aatagtcagg cacagcttgc acctccgccc ggcgcgacca gcctgttccc
     6241 attataccac gccagaaggg gattttacga atcgaaggat gctgcctccg gagcatccac
     6301 agtatgactg atgtgggctc cgcaggacgc caatcgctca gaaaggctgt catagcctcg
     6361 cgtgatgtgc tctacaccgt cgataatggt ttcgccgcgg gccgtcagcc cggccacgac
     6421 gagagcggcc cctgcacgca ggtctggagc ggaaacccgt gcgcctgtca gttcactcac
     6481 gccgcggatt atggcggtcc tgccatcaac cgaaatctgg gcgcccatgc ggttcaattc
     6541 gtccacatag cggaacctgt tgttgaagat gttctcggtg actacacttg tgccacgccc
     6601 caccgtgagc agcgccgcca tgggcgcctg cagatcagtg ggaaatccag ggtaaggcat
     6661 gctcttgact gatatgggcg caggcctgtc acccatgacc acccttacgg aatcctccga
     6721 agcaataatg gccgctccgg cctcggccag cttcgctccg acagcctcca aatgctctgg
     6781 gaccacatca gtgaccgtaa catcgccgcg ggtggcggtt gcagcgatca tgtaggttcc
     6841 cgcctgtatc ctatcgggca ttaccgggta agtagtgctt cccagctcgg gcaggccggt
     6901 gatcctgatg gtgtcagtcc cggcgccgcg aatctgcgcg cccagccttg ttagaaaatt
     6961 ctgctggtcc acgatctcag gctcgcgggc agcgttccgt atcacggtgg tacctgtcgc
     7021 catgcatgct gcagccatca ggttctcggt ggcgcctaca cttgggaaat ccaggtgtat
     7081 ctccgtgccc accaggcgat cggcgcgtcc gctaatcagc ccatgctctt cggaaaactg
     7141 ggcgcccatc gcggtaagcc ccttaagatg caggtctatt gggcgtgacc ctatcgggca
     7201 tccgccggga tacggcatct gaacctcgcc gaatctggcg agcaaaggcc ccagcacctg
     7261 caccgaagca cgcatacacc taaccagctg ctccggcaga cgggtacacg agatcggcct
     7321 cggatcgatg gataggacag aatcgcgccg gctcacaatc acggctccga ccgctctgag
     7381 aaggtcgcac atagtgtcga catcagtgat ggaaggcacg ttggtaatgg tgcactcaga
     7441 ggaagccatt atgcaaagag ccatcaattt gaggcatgag ttcttggcgc cgggcaccga
     7501 cacccttccg cgaagctccc ggcccccctc tattctcagc cactccatgg attccacctc
     7561 gaacccttaa taggcacggc cagcagccac tttagcctcc tactacacgc acctcaggca
     7621 ctagatccac cccgaatttc tcgtacacgg tgcgcctgat cacacccatg aggataagca
     7681 catcgcgagc ggtggcgccg cctgtgttga cgatgaagtt ggcatggacc ggtgatactt
     7741 cggctcctcc cacccggagc cccttgagcc ccgcagcatc tatatacctg ccggcccccc
     7801 gccccgacgg gttctggaat acacatcccg cgctgggcat gtggagaggt tgcttaatcc
     7861 ttctgtcttt catatagccc gctgtgcggg cgcgtatctc gtcggggtcc gcatgacgca
     7921 aggcaaggag cgcctgatat gctatgacat cgccttgcga aagacgactg gacctctgcg
     7981 caaactgcat ctcctcgccg gtcttgaccg aaaccgcgcc atctaggcct atcatcttga
     8041 cctcgcgggt gacatcgccg accgtgtggc tgtacgcccc tgcattcatc gtgagggccc
     8101 cgcctaagga accgggtatt cccacggccc actcaaggcc cgcaagcccg cgctccgcgc
     8161 actctctggc aagcacgggc agggtcaccc cggcgcccgc cctcacgcat tcgccttcga
     8221 atgagatgtc gtcaaggcca ggtccaatcc tcaccacgac tccccggatt ccctcgtccg
     8281 ccacgagcaa gttggttccg ttgccgagca cgaaaacggg cagcccttcg ctccatgccc
     8341 actgcaacac ggtctgcaag gcttccacat catgcggttc caccagcacg tcggctggac
     8401 cgccgatctg aaacgacgtg tatctcttca tcagctcatc aagcatgatt ttcgcgtcgt
     8461 aaaccatcct gagtcgcctg gcttgctcgg ccgtcaccat gctcgtcatc gagttaccac
     8521 cgcccgtctt ccgatggccc gggtcgtgct cccgagcgca gtagctcttc ggcgaaacgt
     8581 cgcgatgtcc tgtatatgtc gcccgcgccc atcgtgacga tggtatcgcc gggggtcata
     8641 acgcttctca ggaaatcctc cagagcgtcc cggtcggcga gatacacaac gggcctgccc
     8701 tcatacgcct cgatactctc agcgagatgc cgcccggtga ccccggggat aggctcttct
     8761 ccagtgcctt catagtaaat ctcggttact gcgatcacat ctgctaagct aaacgagcgg
     8821 acgaattcgt catgcagcaa cttggtcctg gagaaacgtt ggggctggaa cacagccacc
     8881 aacctcccgc ttgccgcctt ccgcaaagca gccaaggtgg cgcgtatctc tgcgggatga
     8941 tgcgcatagt catctaccag cgtcacatcg ggtgtacgtg ccaacacctg gcatctccgg
     9001 tccgcccctg aaaaacgggc cagccccgtc cgcaccacgt caaagtcgat gccgagctga
     9061 tcggcgagag cgatcgcggc tatggcatta ctcatgttat gcatcccggg caccgaaagc
     9121 tcaacctgtc ccatgggcct tccatgatgc gacgccacga agcgcgatcc gaatcccagc
//
LOCUS       DAOTAC010000072         9164 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_6437_length_9164_cov_4.637721, whole genome shotgun sequence.
ACCESSION   DAOTAC010000072 DAOTAC010000000
VERSION     DAOTAC010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 9164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9164
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6437_length_9164_cov_4.637721"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /locus_tag="PLM74_04495"
     CDS             <1..686
                     /locus_tag="PLM74_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42379.1"
                     /translation="FLMIENCGDIYSQYAYANLTWNGAFYDEFFNMYKYTFPEFIQVN
                     MVNPRRIADRAERAAWFYKDVARAFVLGSVFWTELGDRFCEEDDCLLDYFRAALRLRT
                     QAAPYIAHGVYQDDLGIAFATDDGLPVVSGPITASRWILPGGGVLVLISNPEQLSGIE
                     IRVDGPDRHAWVDGAELCVNAGALLAEPTVFGTSAADGSRVTLEVPRSQLSFLSMCPG
                     GGDSGASGP"
     gene            700..1134
                     /locus_tag="PLM74_04500"
                     /pseudo
     CDS             700..1134
                     /locus_tag="PLM74_04500"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1337..2620)
                     /locus_tag="PLM74_04505"
     CDS             complement(1337..2620)
                     /locus_tag="PLM74_04505"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HON42380.1"
                     /translation="MRARFSCTVPQRRIRPALLRVAAVITLAVTSIAFMPVWPASADN
                     PVRAARNVILLIGDGMGSAQVETCRRAAALAGRRLAMDSMPVTSLVITTSAGGEITDS
                     AAAATAMATGRKTVNGRLGIGPDGEYLPNITESVRATGGLIGLVTNCSITSATPAGFV
                     VHIPDRDSGRDIARAMVEAGPDVALGGGAAFFMPTLFERARPALDSALEKGYTVVMEL
                     DELEAAFTLPLLGLFAMTDMPYELDRDLAEEPSLRQMTQKALALLAKSSGDAGFFLMV
                     EGGRIDHACHAMDSNRLIAEVASLDDAVSAALEFARRRGDTLVVVTADHETGQLEVTN
                     DGRIVFGHDGHTEALVPLFAEGPGQEWFSIGVLDNTDIARLIARALCPPGAVSGRGPA
                     TRDRPGRRPSTPGDRMPSPHRRSSSPCEHRFRLRT"
     gene            complement(2649..3509)
                     /locus_tag="PLM74_04510"
     CDS             complement(2649..3509)
                     /locus_tag="PLM74_04510"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HON42381.1"
                     /translation="MKGVRAVGYRRSRWAVLAGRIAVYAIIAVGAVAMAMPFVWLVLS
                     SFKTLQEIVLFPPTFFPRNPTLSNFKQVFDRIPFARYYANSIVTSVIPTAVSVLTCTM
                     AGMGFAKYRFRGRDAIFWTILSTMMVPFPATIVPLYVMISRMRLVDTRLGLIIVGLSS
                     ASGIFMMRQFIMGIPDELLDAARIDGASEFRIFRQIIVPLCKPAIATQALFSFTNHWG
                     AYIWPLIVVNSDELRTLPLAIPYFSGQYYSFQNLINAASLMAVLPVLILFLFTQRYFV
                     QGITITGMKN"
     gene            complement(3506..4411)
                     /locus_tag="PLM74_04515"
     CDS             complement(3506..4411)
                     /locus_tag="PLM74_04515"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HON42382.1"
                     /translation="MVNVETRKYRAYRLQRNAAPYMFILPGTVFYLMVTIIPMMIGFW
                     MSFHNWTIIRPTKPWVGLANYQKVLANPVFWTAMKNTLVYTAGVVPFQMILALLAAVL
                     LNTEIRGRTLFRLLYYLPVVTPLSIAAVIWTWIYHPNLGLLNWFLRSIGFSGRDWLGD
                     PKVAMLSVIIVAIWSGLGYRMVIFLAALQGIPETYYEAAMIDGAGRFDLFRFITMPLL
                     RPATLYVLITSVIGSFQVFGLVNVLTGGGPLDATNVLVMWIHRRAFGDYQFGEAAATS
                     FVLFALILAITAIQWKALGREVTYE"
     gene            complement(4550..5821)
                     /locus_tag="PLM74_04520"
     CDS             complement(4550..5821)
                     /locus_tag="PLM74_04520"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HON42383.1"
                     /translation="MRRETMRALTMVCAVMLILAVVSGTGLAKRKVVLVHWQHHTPAR
                     KDMVEAFAADFMKANPDIEIKIESVPEADYFQKLLPALAAGSGPDTFQIPAGQVLTYA
                     RSGVLQPLDPGVATAKQIEADFVPGSIERLLIEGKYYGFPVDTQTIVLFYNPKLFKEA
                     GLDPDKPPQTWDEAIKYAKLMTKRDASGVTTQMGLATGGYGPVIASLMIQNGVRMWNA
                     QTDLPDFDSPKAAEALQFAADILTAHKVEDTKMGSRWNAFRAEKLGMVYAHPAMIGSF
                     RATVPDLVFRIAEVPAPKAGGSRSTLLSSWAYVVSAKAPSDAATRWIAFLTSADAQRQ
                     WITKTGDLPSRKALLDLTEYKSDPLLAPCMESMKKAVPAPWTTRQLDDTLFRKAYQLM
                     TLNAETAQTAVKWLTAEGIKAEKEQREMAKP"
     gene            6115..7626
                     /locus_tag="PLM74_04525"
     CDS             6115..7626
                     /locus_tag="PLM74_04525"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HON42384.1"
                     /translation="MGFRRHSTFSRSAQTRWAVSFMAAAVLMLGGGAGLRGATFKCVV
                     LAQESTSVAAIDGPRRGDEVGVRMIPGSERGEGEDAITPAAAYVIYINWDAFRRDYHN
                     WANEQGAPGTPNLNRLASMGALFTAARNGFPSITNPMQASIVTGAWPGVHGNVYRYYD
                     VSGNIVRGAGRTCEAETIAEAAAASGLSTASVQQFMLQGRGTADDDPLHLYIQPGGRF
                     EERVAEAVKIIKGQPVRTLGGRIVAAAEIPRFMAIYGDDLDAAGHNSGSGYGAGIAFT
                     YEQWKAKMVRELVRMDAALGTLIRALIDVGIMERTAIILTADHGMTPYWGTSSLSDLV
                     RVLQSLGYKPKALSTDQKAPAGTDVVLVGTGISVQAYFRRQLGPDEYRSLVEAISAQP
                     YVGGCLTRDDLDRIGAHTRSGDLVVWPKPPHHFASNSITFGLGGNHDTLDPSSRNVFL
                     LVSGAGVQHGVVIDHPVEIIDVAPTISRLLGIRPPAQATGRVLDEALRAPDKS"
     gene            7839..8651
                     /gene="rsmI"
                     /locus_tag="PLM74_04530"
     CDS             7839..8651
                     /gene="rsmI"
                     /locus_tag="PLM74_04530"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HON42385.1"
                     /translation="MLRTQAVAGDRRRDGEGTGRDLAGAGKLFVIATPIGNLEDLTFR
                     AKRVLSEVDALACEDTRHTRILLDRYDIPRPRLLFSYHEHNEDRAAQRILGLLAADQN
                     VGLCSNAGYPGISDPGYVVISQAVSRGFAIEVIPGASAVPTALLSSGLPTSSFVFKGF
                     PPRKAGRRRAFLEAERDLPHTLVLYESPHRLAALLQAALEALGDRQAAVCIELTKMFE
                     DVHRGPLSALAKEFEGTKVKGEVAVVIAGNNPKFTADQDGSAGDGLSDERAE"
     gene            8651..>9164
                     /locus_tag="PLM74_04535"
     CDS             8651..>9164
                     /locus_tag="PLM74_04535"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-guanine transglycosylase"
                     /protein_id="HON42386.1"
                     /translation="MRSALNTLHGELPLPAFIPDATRAVVRSADSVDLAEARVPGLMV
                     NTFHLSASPGVSAVASVGGIHSFMGWSGLVAADSGGFQVFSLLSENPALGSVTDRGFV
                     YRRAKGGERQTLTPEKCIERQFRMGADIMFCLDYCTHPGAPASIQSESVRLTVEWARR
                     CQETFLRQADQ"
BASE COUNT         1881 a         2596 c         2820 g         1867 t
ORIGIN      
        1 cgtttctgat gattgagaac tgcggcgaca tatacagcca gtatgcttac gccaatctga
       61 catggaacgg cgcgttctac gacgagttct tcaacatgta caagtacacg tttccagagt
      121 tcatccaggt caacatggtg aacccgcgcc gcatcgccga tagggccgaa agggctgcgt
      181 ggttctataa ggatgtcgcc cgcgcatttg tgctaggatc agtgttctgg acggagcttg
      241 gcgacagatt ttgcgaagaa gatgactgtc tactggacta tttccgcgct gctctgcgtc
      301 tccgcaccca ggcggcgcca tacatcgctc acggtgtcta ccaggacgat ttgggcattg
      361 cgttcgcgac cgatgatggt ctgcctgtgg tatcgggtcc catcactgcg agcaggtgga
      421 tactgccggg aggcggcgtg cttgtgctca tctccaaccc cgagcagttg tcgggtatcg
      481 aaatacgggt tgacgggccg gacaggcacg cttgggtaga tggcgccgaa ttgtgtgtga
      541 atgcaggagc gctgcttgca gagcccacag tgtttgggac ttcggctgcc gatggcagtc
      601 gagttacgct agaagtgccc agatcgcagc tgtccttctt gtcaatgtgc ccaggtggag
      661 gtgactcagg tgcgtcgggg ccctagggta gactaccatg tgcactcatc tgagtcggat
      721 ggcgaagcaa gccccgatca gattccggaa ctcgcgaggc aagcccgcct tgccgcctgc
      781 gcgatcaccg accatttcga tccttatgcc ccgggctacg agccggtacc ggggaagtcc
      841 ctggatgtgg ctcgtctgct atcaatgcta aagtggcgcg acgaatggcg cttgtcatgc
      901 cgagaacacg gcgtggacct gttcgttggg atcgagcgcg gaccggtgcc tgttcctctt
      961 ggccgaacga ctccggacct gataattgcg agtgtacact atctaccggc gaacgtgccc
     1021 gcagtgcgag ggcgtctctt cgatgagacg tactggaatg cctacatgaa aagcgtgttc
     1081 gacgtcgcga gcggcccgca ggtggatgta gtaggacata tggcgggata cctgcccatg
     1141 aatcctatgc tgccgccaat atctactttc gaagagcgcc gcgagattga acgggagatc
     1201 gcgcgcaggt acttcacacg cgattggtat gagaaggtgt ttcgcattgc ggccacgcgt
     1261 ggaatagcgt gcgagctaca ctgcccgacc agaagccccg gccctgacat ggtgaggctc
     1321 gggctcagta tgggagtcaa gttctcagtc ggaagcgatg ctcacatggc gagctggatc
     1381 ggcgatgtgg gctgggcatt cgatctcctg gagtcgatgg gcgccggccg ggccgatctc
     1441 tggtcgccgg gcctcgaccg ctgaccgccc cagggggaca gagagcgcgc gcaatcaacc
     1501 ttgcgatatc ggtattgtcc agcacgccaa tgctgaacca ttcctgcccg gggccctcgg
     1561 cgaagagcgg aacaagggct tcagtatggc catcgtgccc gaacacgatc ctgccgtcgt
     1621 tggtcacttc gagctgccca gtctcatgat cggcagtcac cacgactagc gtgtcgccgc
     1681 gacgtctggc gaactcgagg gctgccgaga cggcatcgtc aagcgaggcc acttccgcga
     1741 tgagcctatt tgaatccatg gcgtggcagg catggtcgat gcgcccgccc tccaccatca
     1801 agaagaaccc tgcatccccg cttgacttgg caagcagcgc cagggccttc tgggtcatct
     1861 ggcgcaggct aggttcctcc gccaaatcgc ggtcaagctc atatggcatg tcagtcatgg
     1921 cgaacagccc gagcaggggt agggtgaacg cagcctcgag ctcatccagc tccatgacga
     1981 ctgtgtagcc tttctccagt gcactatcta gcgcagggcg tgcccgctcg aagagcgtgg
     2041 gcatgaagaa ggctgcgccc cctccaagcg ccacgtccgg cccggcctca accatcgcgc
     2101 gggcgatatc acggcccgag tcgcggtcgg ggatgtgtac tacgaatccc gcaggcgtcg
     2161 ctgaagtgat ggagcagttg gtcaccagcc caatcagccc cccagttgcc ctgactgact
     2221 ccgtaatgtt cggtaggtat tctccgtccg ggccgattcc caggcggcca ttgacggttt
     2281 tgcgaccggt tgccatggcg gtggcggcgg cggccgaatc ggtgatctca ccaccggccg
     2341 aagtggttat cacaagcgat gtgaccggca tcgagtccat cgccagccgc cgtccagcta
     2401 aagccgcagc gcgacggcac gtttcaacct gcgccgaccc catcccgtct ccgatcagca
     2461 atatgacatt gcgggcagca cgaacaggat tgtcggccga tgcgggccag acgggcatga
     2521 acgcgattga tgtaaccgca agcgtgatga cggccgccac ccggagcaag gctggtcgga
     2581 tccgcctttg tggcactgtg catgagaaac gcgcgcgcat cgcaagagcc ctcctgcacg
     2641 ccgcctggct agttcttcat gcccgtgatc gttatgccct ggacgaaata gcgctgagta
     2701 aagaggaaga gaattagaac gggcaaaaca gccattagcg acgcggcgtt gatcaagttc
     2761 tggaaactgt agtactgccc ggagaagtac ggaatggcca gcggcaaggt acgcagctca
     2821 tcgctgttga caacgatgag tggccatatg tacgcgcccc agtggttggt gaagctgaaa
     2881 agggcttgag tcgctatggc gggcttgcac agcggcacga ttatctggcg gaatatacgg
     2941 aactcgcttg ctccgtcgat ccttgccgcg tcgaggagtt cgtcaggaat gcccatgatg
     3001 aactggcgca tcatgaatat ccccgaggcg ctggagagcc cgactattat caggcccagt
     3061 ctcgtgtcca ccaacctcat cctagaaatc ataacataca agggcactat cgtcgcgggg
     3121 aacgggacca tcatggtcga aagaatggtc cagaagatgg cgtctcttcc ccggaacctg
     3181 tacttggcaa aacccattcc cgccatggtg caggtcagga cgctcacggc ggtgggtatg
     3241 actgaagtga ctatactgtt tgcgtagtag cgggcaaacg gtattctgtc aaacacctgt
     3301 ttgaagttgg acagcgttgg gtttctcggg aagaacgtcg gaggaaacaa cactatttcc
     3361 tgcagcgtct tgaaggaaga gagtacaagc cagacgaagg gcatggccat tgccactgcg
     3421 ccgacggcga taatggcgta aacggcgatg cgccctgcca gcacagccca tctagatctg
     3481 cgatagccca cagccctaac gcctttcatt cgtaagtaac ctccctcccc agagctttcc
     3541 actgaatcgc ggtgattgcg agtatgaggg caaacaggac aaacgacgtg gcggccgctt
     3601 cgccgaattg gtagtcgccg aacgccctgc gatgaatcca catgacaagc acgttggtgg
     3661 catccagggg gccgccgccg gtcaacacgt tgaccaggcc gaacacttga aatgatccga
     3721 tcacgctggt tatcagcacg taaagggtgg ccggtctgag caagggcatg gttatgaacc
     3781 tgaacaggtc aaaacggcct gcaccgtcga tcatggctgc ttcgtagtat gtttcgggta
     3841 tgccctgaag cgcagccagg aatatgacca tcctgtagcc gagtcccgac cagatggcaa
     3901 cgattatcac cgagagcatg gcaactttcg gatcgcccag ccagtcgcgg ccggaaaatc
     3961 cgatgcttcg caggaaccaa ttgagaaggc ccaggttggg gtggtagatc catgtccata
     4021 tgacagccgc gatcgagagg ggagtgacta cgggcaagta gtagaggagg cggaagaggg
     4081 tccgtccccg gatttccgta ttgagcaaca ccgctgccag gagggccagg atcatctgga
     4141 acggcacaac cccggcggta tagaccagtg tgtttttcat ggccgtccag aataccgggt
     4201 ttgcaagtac cttctggtaa ttcgccagtc caacccatgg cttggtcggg cggatgatag
     4261 tccagttgtg aaaactcatc caaaagccga tcatcatagg gattatcgtt accatcagat
     4321 agaacacggt gccgggaagg ataaacatgt atggagccgc attcctctga agccgatacg
     4381 cgcggtactt acgcgtctcg acgttgacca cgaagactcc cctccttgct ggttcgagaa
     4441 gcggacccct cctgccttgg gcgccggcat atagagactg cgtttgggct attagtcggg
     4501 gcccgcctcg catcgcgatg tcaaagggtc gaaaccatgc caggttccgt tacggctttg
     4561 ccatctccct ttgctccttc tctgccttga tgccttcggc tgtgagccac ttgacggccg
     4621 tttgcgcggt ttccgcattg agcgtcatga gctggtatgc tttcctgaaa agcgtgtcgt
     4681 cgagctgccg agtggtccac ggggcaggaa ccgctttctt catcgactcc atacaaggcg
     4741 cgagcagcgg gtcgctcttg tactccgtga ggtccagcag ggctttccgg gagggcaggt
     4801 cgcctgtctt cgttatccac tgtctctgtg cgtctgctga ggtcaaaaac gcgatccatc
     4861 gggtagctgc atctgacggt gccttcgccg acacaacgta tgcccacgat gaaagcagcg
     4921 tactcctgga ccctcctgcc ttgggcgccg gcacttcggc tattctgaac actagatccg
     4981 gcactgtggc cctgaaactc cctatcatgg cggggtgagc atagaccatg cccagctttt
     5041 ccgcgcggaa cgcgttccac ctggagccca tcttggtgtc ttcaacctta tgggccgtga
     5101 ggatgtcagc ggcaaactgc agggcctcag ccgccttcgg cgaatcgaaa tccggaaggt
     5161 cggtctgtgc gttccacata cggacgccgt tttggatcat caaagatgcg atgaccgggc
     5221 cataaccgcc ggtggccagg cccatctgag tggtgacgcc cgacgcgtcg cgtttggtca
     5281 tgagcttggc atacttgatc gcctcatccc aggtctgggg aggcttgtcg gggtcgagcc
     5341 ccgcctcttt gaagagcttg gggttgtaga acagcacgat ggtctgagtg tccacaggga
     5401 atccgtagta cttgccctcg atcagcagcc gctctataga cccgggcaca aagtcggcct
     5461 ctatctgttt ggcagtggct acgccggggt ccagaggctg cagaactcct gagcgagcat
     5521 aagtaagcac ctggccggcg ggtatctgga atgtatcggg cccagagccc gcggcaagcg
     5581 ccggcagcaa cttctggaag tagtcagctt cgggcacaga ctcgatcttg atctcgatgt
     5641 cgggattggc tttcatgaaa tcggccgcga atgcttcaac catatccttg cgagcaggag
     5701 tatggtgttg ccagtggacc aggactacct tgcgcttggc caggcccgtg cctgaaacta
     5761 cggccagaat gagcatgact gcgcagacca tggtgagcgc gcgcatggtt tcccttctca
     5821 tgagctttcc ctccctgatc gcgctgacgc tgtgggcgcc aggcgtttgt tcataatcaa
     5881 tataccagtt gagaatgaca cagtcaatga catttgcggc gctataaggc atcacaacat
     5941 acagttacct gcatagagcg tagttgcgaa ttccgggata ctaacttatc atggagtaaa
     6001 gatacgccat tgatgccttg tatcatccac aaacgccacc gattggcgaa ttctgtcggg
     6061 cttgatcggt gttttggggc cgcgaatcaa gccgcccaac aggaggttgg catattgggt
     6121 tttcgcaggc actcaacctt cagtcgatct gcgcagacga ggtgggctgt atcgttcatg
     6181 gccgccgctg tcctaatgct aggagggggc gcgggccttc gcggcgcgac gttcaagtgc
     6241 gtagttcttg cacaagagtc gacgagcgta gccgctatcg atgggcctcg gagaggcgat
     6301 gaggtaggtg tgcgaatgat tcccggaagt gagcggggcg agggggaaga cgcgattacc
     6361 cctgcagccg cctatgtgat ctacatcaac tgggacgcat tcagacgcga ttaccacaac
     6421 tgggcaaacg aacaaggcgc gccgggtacg cctaatctca atcgccttgc ttccatgggg
     6481 gccctattca cggcggcacg caacggtttc cccagcataa ccaatccgat gcaggccagt
     6541 atagtcactg gtgcatggcc tggggtccac gggaacgtct acagatacta cgacgtctcg
     6601 ggaaacatcg tgcgaggtgc cggccgcacc tgcgaggccg agactatcgc cgaggcagct
     6661 gccgcatcag ggctgtcaac cgcctcggtg cagcaattca tgttacaggg acgcggaacg
     6721 gccgatgacg atccgctgca tctttacatt cagccgggcg gcagattcga ggagcgggtc
     6781 gccgaagctg tcaagataat caaggggcaa ccggtacgca cactgggcgg cagaattgta
     6841 gccgcagccg agatcccgcg gttcatggcc atctacggcg atgacctgga tgctgccggc
     6901 cataattcgg gctctggata cggggcaggt atcgcgttca catacgaaca gtggaaggca
     6961 aagatggtgc gcgaactagt ccggatggac gcggccctag gtacactgat tcgcgcgctg
     7021 atcgatgtag gcatcatgga gcgaacggcc atcattctga ccgcagatca cggcatgact
     7081 ccgtactggg gaacctccag cctttcggat cttgttcgcg tgctgcaaag cctcggctac
     7141 aagcccaaag cattgagcac agatcagaag gcgcctgccg ggacagacgt agtgctggtg
     7201 ggaacgggta tttctgtgca ggcatatttc cggcgacaac ttggccctga tgagtatcgt
     7261 agcctagtcg aagccatatc tgcccagcca tacgtgggcg gttgtcttac ccgcgatgat
     7321 ctggaccgga taggcgcgca cacccgctcg ggcgatctgg ttgtctggcc caaacctcct
     7381 caccacttcg cgagcaacag catcacattc gggcttggcg gcaaccatga taccctcgat
     7441 ccgtccagcc gaaacgtgtt cctgctcgtg agcggggccg gagtgcagca cggagtcgtc
     7501 atcgaccatc ctgtagagat catcgacgtg gcgcccacca tctcgaggtt gctgggcatc
     7561 aggccaccgg cccaggccac cgggcgtgtg ctggacgagg cgcttcgagc gcctgacaag
     7621 tcctagccgt agcgaccgtt cctatgcgct cacaacgtca tatcgagaag tcatgtggta
     7681 agtcaagcag gaagcaggcc cggaggcgag tgggcccggc aggtcggtgg caagtagata
     7741 ccgagcgcga tgggttgctg agttgggctg ctgggttgag ttgactgctg cagacgtcgc
     7801 tgttatcatc gggttggcaa caggcgcggc gccgcatggt gctgagaacg caggccgttg
     7861 caggggaccg acggcgggat ggggaaggga cgggacggga tttggccggc gctggtaagt
     7921 tgttcgtgat tgcaactcca atagggaatc tcgaagacct cacgtttcgc gcgaagcggg
     7981 tcttgagcga agtcgacgcg ctggcatgcg aggacacgcg tcacactcgc atcctccttg
     8041 accgatacga catacccagg cccagactgt tgttttccta tcacgagcat aacgaggatc
     8101 gggcggcgca gaggattttg ggactccttg cggctgacca gaacgtaggc ctgtgctcga
     8161 atgccgggta tccgggcatc agcgatcccg gctacgtagt gatcagccag gcggtgtctc
     8221 gcgggttcgc gatagaggtg ataccgggcg catccgctgt gccgacggcg ctactgtcat
     8281 caggtctacc cacgtcgtca tttgtcttca agggattccc tcctcgaaag gccggtcgca
     8341 ggcgcgcatt cttggaagcc gaacgtgatc tcccacatac gcttgtgctc tacgagtcac
     8401 cccaccgtct ggcggcgctt ctgcaggccg cgctggaggc gctgggcgat aggcaggccg
     8461 cggtctgcat tgagttgacc aagatgtttg aagacgttca tcgaggccct ctatcggctc
     8521 tggccaagga gttcgagggc acgaaagtca agggagaggt ggctgtcgtg atcgccggga
     8581 acaaccccaa gttcacagca gaccaagatg gctccgctgg cgatggcctc tccgatgaga
     8641 gggcagaatg atgagatcgg cgctcaatac gctccatggc gaactgccgc ttccggcatt
     8701 catccccgat gcaacccggg ctgtcgtgcg ttcggccgac tcggttgatc tggcggaagc
     8761 acgcgtgccg gggttaatgg tgaatacgtt tcacctatcc gctagtcccg gggtctcagc
     8821 agtggcgtcg gtgggaggca tccattcctt catgggctgg agtggtctgg ttgcggccga
     8881 ttccggaggg tttcaggtct tttctctgct ttcggagaac cctgctctgg gcagcgtgac
     8941 agaccggggt ttcgtgtacc gtcgtgctaa gggcggggag aggcagacgc ttaccccgga
     9001 aaaatgcatc gaacggcaat tccggatggg cgcagatatc atgttctgcc tggactactg
     9061 cacgcatccc ggcgcgcctg ccagtatcca gtcggagagc gtacggctca ctgtggagtg
     9121 ggcgcggcgg tgccaggaaa cgttcctgcg ccaggcggac cagc
//
LOCUS       DAOTAC010000073         9089 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_6511_length_9089_cov_5.070733, whole genome shotgun sequence.
ACCESSION   DAOTAC010000073 DAOTAC010000000
VERSION     DAOTAC010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 9089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9089
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6511_length_9089_cov_5.070733"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(401..1417)
                     /locus_tag="PLM74_04540"
     CDS             complement(401..1417)
                     /locus_tag="PLM74_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009674795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HON42387.1"
                     /translation="MTEGSTVWRIINSPAADGATNMAIDESILEAVRQGQSPPTLRLY
                     TWKPACLSIGRFQRAARSVEWDALTSLGYDFVRRPTGGRAVLHDDELTYAVIADAAGL
                     ARAESGKAALVCGSSESNADNSERDSRRTEARTGTRHGTGTRIGTGTGVLDTYLALSR
                     GLAAGLARMGIPVELAPRDAGRAHRGCGREGESLGGTESGGRVGGSAACFDSPSAYEL
                     LAAGRKIVGSAQVRRGSTFLQHGSIPISMNFAAAEAAMGMPKGSGVDLSRCMATVGEF
                     VPGFPADISSEQLLKQLADAIVAGFVYEVMQTTRPGELTPMELELASELRVKYESEER
                     NLAR"
     gene            complement(1414..2493)
                     /locus_tag="PLM74_04545"
     CDS             complement(1414..2493)
                     /locus_tag="PLM74_04545"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HON42388.1"
                     /translation="MGQDRLGKVRVSAGTASVLGLSPIASFDEPTCAYVMIGERCTRD
                     CAFCAQARSSAAPGDRLSRVTWPEFSADDLAQALGAAAGEGKVQRACAQVVGTPGAFE
                     KAIAAVNLLKSAVGPDVPVSVSYSATARIEQVEALMEAGAERVALPIDACNDQLYRDI
                     KGGHDMSRALSLIGECARRFPGRVSTHLIAGLGETEEEIISLAASMYQEGVTVGLFAF
                     TPLPGTRLAHRHPPDLSSYRRIQLCCYLLKRAYMEQPAEGPPGFQFSSGRLTGISLRL
                     SVARVGARWGEAFQTSGCPGCNRPYYNERPGQIPYNYPRPLTPAESADAVAAALNGLE
                     LEGARPADTGLTQRESASTERRPAR"
     gene            complement(2717..4183)
                     /gene="gcvPB"
                     /locus_tag="PLM74_04550"
     CDS             complement(2717..4183)
                     /gene="gcvPB"
                     /locus_tag="PLM74_04550"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013144752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HON42389.1"
                     /translation="MKGPQPLIFELSSPGRTAMSLPAPEVPTPDVGSLLPAQALRHDP
                     PGLPEVSEVDVVRHFVNLSQLNHSVDNGFYPLGSCTMKYNPKVNEDAARMPGFARIHP
                     DQDEATVQGAMELLYSMDRYLSELCGMSRFTFQPAAGAHGEITGLFVIRAYHESRGEH
                     RTKVLVPDSAHGTNPASAAGAGMEIVVVKSDERGNVDVDDLKSKVGPDTAALMLTNPN
                     TLGLFEENVREIAALVHGCGALLYYDGANANAIMGKSRPGDMGFDVVHLNVHKTLSTP
                     HGGGGPGAGPVGVKSFLEPFLPAPLIKLNDATGMYAWDYDRPQSIGRVHSFNGNFGVV
                     VKAWAYIRSMGPDGLRTASEHAVLTANYVMRRLQPYYDLPYDRACKHECVLAGTRQKR
                     EHGVRTLDMAKRLLDFGFHAPTIYFPLIVDEAMMIEPTETESRETLDRFIDAMIKIAK
                     EAEEHPEVLHDAPHHTPVGRLDETAAARKPILRYTKES"
     gene            complement(4180..5538)
                     /gene="gcvPA"
                     /locus_tag="PLM74_04555"
     CDS             complement(4180..5538)
                     /gene="gcvPA"
                     /locus_tag="PLM74_04555"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003332441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HON42390.1"
                     /translation="MGSPHRYIPNTRADVEAMLKAVGADHIDDLFADIPEEFRLDRPL
                     TMPAALSEAELMSELTRLASKCADADGYASYLGAGIYDHYIPSVVDHLISRSEFYTAY
                     TPYQAEVSQGTLQAIFEYQTMISELTGMEVSNASMYDAGTGSAEAAIMACASTRRNKV
                     LVSRAVNPRYRQVLQTYAAPRGIDVVEIDFCKHRGVTPVSRITTDLAADAACLIVQSP
                     NYFGIVEEMEQLGAAIHAAGGLFVAVVNPISLGILAPPGSYGADIAVGEGQPMGIPMS
                     FGGPLLGFFATTGKLMRKMPGRLVGQTVDSSGRRGFVLTLQAREQHIRRESATSNICS
                     NEALCALASTIYMSVMGPAGLRKVAETCLYRAHYLRDRIESIAGFTIPLSGPFFNEFV
                     VRSSVCADDMNEELLAAGIIGGKSIECDYPELSGCTLWAVTERRTREQLDSLIRELEV
                     LA"
     gene            complement(5538..5918)
                     /gene="gcvH"
                     /locus_tag="PLM74_04560"
     CDS             complement(5538..5918)
                     /gene="gcvH"
                     /locus_tag="PLM74_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013756493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HON42391.1"
                     /translation="MSAVKYTRDHEWVKVEGKIATVGITDFAVQELGDLVYVELPAVG
                     DEVRAGERLAAVESVKAASDVFAPVSGEVVEVNEALEDEAGLLNESPMDEAWIARIEM
                     SDPKELDALMDRAEYDEFVKEASH"
     gene            complement(5926..7035)
                     /gene="gcvT"
                     /locus_tag="PLM74_04565"
     CDS             complement(5926..7035)
                     /gene="gcvT"
                     /locus_tag="PLM74_04565"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HON42392.1"
                     /translation="MLELRKTPLYDAHVEAGGRMVDFGGWALPVQYTSILEEHRAVRE
                     RAGLFDVSHMGEIHLTGPDAMAFIQHLVTNDCSQMQVHQIVYSPMCYPDGGVVDDILI
                     YRVCENDYWLVVNASNTDKDYDWITRNASGYDVTVSNISSKVAQVAIQGPVAQTILQR
                     NTDCDLDSIKFFWCRPETTVAGRKCLVSRTGYTGEDGFEIYCKPEDARHIWDTLLEAG
                     KTDGLIPAGLGARDSLRFEAALPLYGHEISQDISPLEGNLGFFVKLGKSEFIGREALS
                     AQKERGLSSRVVGLELAGRGVPREGYPVMAEGREIGRITSGMFSPTFNKGLAMALITT
                     EHARPGTALAIDIRGKAVPATVVKTPFYKKAYRKE"
     gene            7250..8623
                     /gene="lpdA"
                     /locus_tag="PLM74_04570"
     CDS             7250..8623
                     /gene="lpdA"
                     /locus_tag="PLM74_04570"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HON42393.1"
                     /translation="MHDIAIIGGGPGGYVCAIRAAQNGLSVALVEKGQLGGVCLNRGC
                     IPTKALAHTADVMDGASRAAELGLVIPEVRLDFARVMARKDKVVARLRGGVGLLLKRR
                     KVDVSSGVGTLAGTRAVEVTAPDGSVTRIEARNIVIATGSEPVAPRMFGYDGVNMLTS
                     DDVLKLKELPQSMLIVGAGVIGCEFASIFRTFGCEVTMVDIMPNILPMVDEDVANTVR
                     AAFAKRGIKIHTGTKITSVKVADGQVEATCEDGKTLIAQKGVLSVGRRPTTGGVGAAD
                     VGIDTGDAGEIIVDSQMRTNVPGIWAIGDVTGKSQLAHVASAQGLAVAANVAGQSRSV
                     EYYAVPNCIFTNPEVAAVGVSERNAQESGIDYRVGKFPFVASGKAVAMGAPEGFVKVI
                     ADGAGRVIGGQVVGAHASDLIAEIALAVSRRLKLEDISETIHAHPTLAESTAEAVEAT
                     VGQAIHI"
     gene            8767..>9089
                     /locus_tag="PLM74_04575"
     CDS             8767..>9089
                     /locus_tag="PLM74_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42394.1"
                     /translation="MADERVGVFRREKKRAGDASGPGKTHGDAVNPRSRNRTPRPQPR
                     SQSKHRTQPPPPRLSDHARLRSRLRVARALTLAAFGAAGFLAGAARLFGAYSPFGIVA
                     VAALAA"
BASE COUNT         1674 a         2815 c         2758 g         1842 t
ORIGIN      
        1 caggatgccc cagaaccccg ctagagccca ccacagggga acgtcacgcc cagaaatacc
       61 catggatacg ggtaatgcac agatggggat gggttcattt cattatcggc ttggttggct
      121 acagtaggtc aagggccgtt tgacagcttt tttggacgaa accaagagat acgcttgtac
      181 agccgcagct agcgtgtaac aagacggtac ggtttctgcg ccaacctgca agggaagagt
      241 acggtctgct ctgtcgtagt aggcattggc caccccgggg aaccgcacag ccgtgccatg
      301 caacgcagct gccaaccgat tccgagcact cggagtcgtt tcgcgggcgg gaggcggttg
      361 ggtcgggcaa gcagaggtgt ggtgtacagc ctgtcggacg ctacctcgcc aggttccgct
      421 cttcgctttc gtacttaacg cgcaattccg aggccaattc cagttccatc ggggttagtt
      481 cgcctgggcg ggtggtctgc atcacttcgt agacgaaccc agcgacgatg gcatcggcca
      541 gttgcttgag caattgctca gaggagatgt ccgccgggaa ccccggaacg aactcgccca
      601 ccgtggccat gcatcgcgac agatccacgc ccgagccttt cggcataccc atagcagctt
      661 ctgcggccgc gaagttcatg gatatgggga tggacccgtg ctgcaggaag gtcgagccgc
      721 gcctgacctg ggcggatccc acgatcttgc gccccgccgc cagaagttcg taggctgacg
      781 gggaatcgaa gcacgctgcc gatccgccca cacgacctcc cgattcggtc ccacctaggc
      841 tttcaccctc gcgaccgcaa ccgcgatggg cccgaccggc atcgcgcggc gcgagctcca
      901 cggggatgcc cattctagcc aagccagccg cgagccctcg gctgagggcc aggtaggtat
      961 ctagtacccc ggttccggtt ccgattctgg ttccggttcc atgtctcgtt ccggttctcg
     1021 cctcggttcg gcggctgtcg cgttcactgt tgtctgcgtt cgactcgctg ctaccgcaga
     1081 ccagggcggc tttgccgctc tcggcgcgtg ccaatccggc cgcgtccgca atcacagcgt
     1141 aggtcagctc gtcatcgtgc aacaccgccc gacctccagt gggtcgcctg acgaagtcat
     1201 aacccaggct tgtcagggca tcccactcca ccgacctcgc ggcgcgttgg aaccggccta
     1261 ttgaaaggca ggcgggcttc caagtgtaga gccgaagcgt cgggggcgat tgcccctggc
     1321 gcacggcctc gagtatcgat tcgtcaatgg ccatgttggt agctccatca gctgcgggcg
     1381 agttgatgat cctccatacg gtagaacctt ctgtcatcgg gccggtctcc tctcagtact
     1441 cgccgattcc ctctgggtga gcccggtgtc ggccggcctc gcgccttcca actccaagcc
     1501 gttgagcgcc gccgcaacgg catcggcgct ttccgccggg gtgagcggcc gtgggtagtt
     1561 gtacgggatc tggcctgggc gctcgttata gtaaggccga ttgcagccgg ggcatccgga
     1621 tgtctggaag gcctcgcccc accgggctcc cacgcgagcc acagagaggc gaagtgagat
     1681 gcccgtcagc cgacctgatg agaactggaa acccggaggt ccttcggcag gctgctccat
     1741 atacgcacgc ttcagcaggt aacagcagag ctggatccgg cggtaggagc ttaagtcagg
     1801 aggatgcctg tgggctaggc gagtgcctgg aagcggggtg aaagcgaaca gccccacggt
     1861 gacgccctct tggtacatgg atgctgctag ggagatgatc tcctcttcgg tctcgcccag
     1921 tccggctatg aggtgggtgc tcacgcgtcc ggggaagcgg cgggcgcatt cgccgatcaa
     1981 gctaagcgcc cgcgacatgt catgccctcc cttgatatcg cggtacagct gatcgttgca
     2041 ggcgtcgatg gggagcgcta ccctttcggc gcccgcttcc atcaaagctt ccacctgctc
     2101 aattcgagcc gtggccgaat aggacacgct cacaggcacg tccggcccga ctgcgctctt
     2161 gagcaggttt acggcggcga tcgctttttc gaacgcccct ggcgttccca caacctgcgc
     2221 acaggccctc tgcactttgc cttcgccggc cgcagcgccg agagcctggg ccagatcgtc
     2281 tgctgagaat tccggccagg tcacccggga gagcctatcg cccggcgcag cgctgctacg
     2341 tgcctgagcg caaaaagcac agtcacgggt gcatcgttcg cctatcatca cgtatgcaca
     2401 cgttggttcg tcaaacgatg cgatgggcga aagtcccaga acgcttgcgg ttccggccga
     2461 cactctcacc ttgcccagcc tgtcttgtcc catgctacat ccactcccgc gaactgatac
     2521 ttgtgaactg atacttgcga gaaccgttga ctcctcggca gcgaggcgtc aacggttctc
     2581 cactagctaa tctgtcgcca catgttggcc tatcgctttc ggatccgacc cgaggacgga
     2641 ccccgtcgat tccggggacc cttccaattc cggggacccc tgaaattccg cagtctccta
     2701 cgattccgtg gttcgcctac gattccttag tgtaccgcag gatgggctta cgcgctgcgg
     2761 cagtctcgtc caatcgacct acgggcgtgt gatgcggtgc gtcgtgcagc acctcaggat
     2821 gctcttcggc ttcctttgcg atcttgatca tcgcgtcgat gaagcgatcc aacgtctcgc
     2881 ggctctccgt ctcagtaggc tcgatcatca tcgcctcatc tacgatgagc gggaagtaga
     2941 tcgtcggggc atggaatccg aagtccagca ggcgcttggc catgtccagc gtgcgcacgc
     3001 catgctcgcg cttctgcctc gtccctgcca gcacacactc gtgcttgcac gcgcggtcat
     3061 acggaagatc gtaatagggc tggagcctcc gcatgacgta attggcggtc agcactgcgt
     3121 gctcgcttgc ggtcctgagg ccgtcgggcc ccatggaccg gatgtaggcc caggccttga
     3181 ccacgacgcc gaagttgccg ttgaaggaat ggactcgccc tatcgactgt ggcctgtcgt
     3241 aatcccatgc gtacatgccg gtggcgtcgt tcagcttgat caggggcgcc ggcaggaacg
     3301 gctccaggaa tgacttgacc cctaccggcc ctgcgcctgg gcctccgccg ccgtgcggtg
     3361 tggacagggt cttgtgcacg ttgaggtgga ctacgtcgaa ccccatgtcg ccagggcgcg
     3421 acttgcccat tatcgcgttg gcgttggcgc cgtcgtaata gagcagcgct ccgcacccgt
     3481 ggaccaacgc ggctatctca cgcacgtttt cctcgaacag gcccagggtg ttggggttgg
     3541 taagcatcag cgcggctgta tcaggcccga ccttggactt gaggtcgtct acgtccacgt
     3601 tgccccgttc gtccgacttg actaccacga tctccatccc ggcgcctgca gcgctggccg
     3661 ggttagtgcc gtgggccgag tcgggcacaa gcaccttcgt gcgatgctcg ccgcgcgatt
     3721 cgtggtacgc ccggatcaca aacaggccgg tgatctcccc gtgcgcgcct gccgccggct
     3781 ggaatgtgaa gcgactcatt ccgcatagct ccgacaggta tcggtccatg gagtaaagga
     3841 gttccatcgc tccctgcacg gtcgcttcat cctgatcagg gtggattcga gcgaaaccgg
     3901 gcatgcgcgc ggcgtcctcg ttcacttttg ggttgtactt catagtgcac gagcccagcg
     3961 ggtagaatcc gttgtctacg gagtggttaa gctgcgagag gttgacgaaa tgcctcacta
     4021 catcaacctc gctcacctcc ggcaggcctg gagggtcatg ccttagggcc tgcgccggga
     4081 ggagcgaacc cacgtccggc gtgggcacct ccggggccgg aaggctcatc gcggtccgcc
     4141 ccggtgaaga gagttcgaag ataagcggct gcgggccttt catgctagca cctccagttc
     4201 ccggatgagt gagtcgagct gttcgcgagt gcgcctttcg gtcactgccc acagcgtgca
     4261 ccctgaaagc tccggataat cgcactcgat agacttgccg ccgattatcc cggcagccag
     4321 aagctcttca ttcatgtcgt cggcgcacac cgaagagcgc acaacgaact cgttgaagaa
     4381 tggcccggaa agggggattg tgaagccggc gatcgactct attcgatcac gcagatagtg
     4441 ggctctgtac aggcaagtct cagccacttt gcgcagcccg gccggcccca tcacgctcat
     4501 gtatatggtt gatgctagag cgcacagggc ctcattggaa cagatgttgc tggtcgcgct
     4561 ctcgcgccgg atgtgctgct cgcgtgcctg aagcgtgagc acgaaccctc gccgaccgct
     4621 gctgtctacc gtctggccaa ctagacgtcc cggcatcttt cgcatcagct tgccggttgt
     4681 tgcaaagaag cccagcagtg gaccgccgaa gctcatgggt atccccatgg gctgcccttc
     4741 tcccacggcg atgtcggcgc cgtacgatcc aggcggcgcc aagatcccga gcgagatcgg
     4801 gtttacaacc gccacgaaca ggccgcccgc ggcatgaatc gccgctccta gctgctccat
     4861 ctcctccacg atgccgaagt agttgggcga ctgaactatg agacaggcgg catcggccgc
     4921 cagatctgtc gttatacgcg agaccggggt tacgccccga tgcttgcaga agtcgatttc
     4981 gaccacgtct atgccgcggg gcgcggcgta cgtctgcaga acctgccggt acctggggtt
     5041 aactgccctc gacacaagca ccttgtttcg cctcgtggag gcgcatgcca ttatggccgc
     5101 ctctgcactg cccgtgcctg cgtcgtacat ggaggcgttg gacacctcca tgccggtaag
     5161 ctccgagatc atggtctggt attcgaagat cgcctgcagc gttccctggc ttacctccgc
     5221 ctgatatgga gtgtaggctg tgtagaattc gctacgactg attaggtgat ccactacgct
     5281 ggggatgtag tggtcgtata taccggcccc gaggtaggat gcgtatccgt cggcatcggc
     5341 gcactttgac gccagacgcg tgagttccga catgagttcg gcctcactaa gcgctgccgg
     5401 catggtcagc gggcggtcca acctgaactc ctccggaatg tcggcgaaaa ggtcgtcaat
     5461 gtggtccgcg cccacggctt tgagcatcgc ctcgacatcg gcccgcgtat tcggaatgta
     5521 cctgtgcggc gagcccatca gtggctggcc tccttcacga actcgtcata ctcggcccgg
     5581 tccatgaggg cgtccagctc tttggggtcg ctcatctcaa ttcgggcgat ccatgcttca
     5641 tccatggggc tctcgttcag caggccggct tcgtcctcga gagcctcgtt cacctcgaca
     5701 acttcgcccg acacgggggc aaacacgtcc gaggctgctt ttaccgattc cactgcggct
     5761 agtcgctcac cggctcggac ctcatcgcca actgccggga gctcaacgta gaccaggtcg
     5821 cccagttcct gcacagcgaa gtcggtaatg ccgacggtgg ctatcttccc ctcaactttg
     5881 acccattcgt ggtcgcgagt atacttcact gcagacattc tcacactact ccttcctgta
     5941 agccttcttg taaaatggag ttttgactac tgtggccggc acggccttgc cccgtatgtc
     6001 gatggcgagc gctgtcccgg gccgcgcgtg ctcggtggtt atcagggcca tggcgagacc
     6061 cttgttgaaa gtcggggaga acatcccgct cgtgatgcgg cctatctccc gaccctcggc
     6121 catcactggg tagccttcgc gcggcacgcc gcgcccggcg agctcgagac ccaccacacg
     6181 cgagctcagt cctcgctctt tctgggcgga aagcgcttcg cgtccgatga attcggattt
     6241 gcccagcttg acgaagaagc ccagattgcc ctcaagcggc gagatatcct gtgagatctc
     6301 atgaccgtat agaggcagag ccgcctcgaa acggagggag tcgcgtgcgc ccagaccagc
     6361 tgggatcagc ccatccgtct tgcccgcctc caacagggta tcccagatat gtcgggcatc
     6421 ttccggcttg cagtagatct cgaaaccatc ctcgccggtg tatccagtgc gcgacaccag
     6481 gcattttcgc ccggcgaccg tcgtctcggg tctgcaccag aagaacttga tcgagtcgag
     6541 gtcgcagtca gtattcctct gcagaatggt ctgcgctaca gggccctgta ttgccacttg
     6601 agctaccttc gaggagatgt tgctgaccgt cacatcatat cctgacgcat tgcgagtgat
     6661 ccagtcatag tctttgtcag tattcgaggc gttaactacc aaccaatagt cgttttcgca
     6721 tactcggtag atcagtatgt cgtcgactac acctccgtcg gggtagcaca tggggctgta
     6781 aacgatctgg tggacctgca tctgcgagca gtcattggtc accagatgct ggatgaacgc
     6841 catggcgtcc ggccctgtca ggtgaatctc acccatatgc gacacatcga atagccctgc
     6901 tcgttccctc acagcgcggt gttcctcaag tatgctggtg tactgcaccg gcagtgccca
     6961 gccgccgaag tcaaccatac gcccgcctgc ctctacatgc gcatcataca gtggggtttt
     7021 gcggagctcc aacatcacgc cacctcctca ttccttacca caaacgtgct gtgattgata
     7081 ttcattatgc cttcctaaag tccttctgac ttgccggtcg atgagtgtgt ggattgaccg
     7141 gcgcccgcag gcgcttgccg caaagtggga ggaacgggta tcataagggg cgaatacaag
     7201 ggcaggcttg cgctgtcgtc agcggccaat agctaggagg aacaagagta tgcacgatat
     7261 cgcaataatc ggcggaggtc ccgggggtta cgtatgcgcc atcagggctg cccagaacgg
     7321 tctgtcagtt gcactagtgg aaaaaggcca gctcggaggt gtatgtctca accgtggctg
     7381 tatacccact aaggctcttg cgcataccgc cgacgtcatg gacggggcga gtcgcgccgc
     7441 agaactgggc ctcgtcatac cggaagtgcg gctggatttc gctcgagtga tggccagaaa
     7501 agacaaggta gttgcccggc tgcgcggcgg cgtcggtctg ctgctaaaga ggcgcaaggt
     7561 ggatgtgtcg agcggcgtgg gcacgcttgc cggcacgcga gccgtagaag tcacggcccc
     7621 tgatgggtcg gtcacgcgca tcgaggcgcg caacattgtc atcgctaccg ggtctgagcc
     7681 tgttgcgcct aggatgttcg ggtatgacgg cgtcaacatg ctcaccagcg acgatgttct
     7741 gaaactgaag gaactgccgc agagcatgct gattgtggga gccggcgtaa tcggatgcga
     7801 attcgccagc atctttcgga cattcggctg cgaggtcacc atggtggata tcatgcccaa
     7861 catactgccc atggtcgacg aagacgtggc taacacggtg cgggcggctt tcgcaaaacg
     7921 gggcatcaag atacatactg ggactaagat aacgtcggta aaggtcgccg acgggcaagt
     7981 cgaagccacc tgtgaggatg gcaagaccct gattgcgcag aagggtgtac tatcggtggg
     8041 gcgccgtccg accacgggcg gggtaggcgc tgccgatgta ggcatcgaca cgggcgatgc
     8101 aggcgagatc attgtggact cgcagatgcg aaccaacgtg ccgggaatct gggctattgg
     8161 cgacgttaca ggcaagtcgc agctggctca tgtggcctct gcacagggct tggcggtggc
     8221 agccaacgtg gccggccaga gtcgcagcgt ggagtattac gccgttccga actgcatatt
     8281 cacaaaccct gaggttgccg cggtaggtgt gtccgagcgg aacgcgcagg agtcggggat
     8341 cgactatcgg gtgggcaagt tccccttcgt tgcttcgggc aaggcggttg ccatgggggc
     8401 gccagagggt ttcgtcaagg tcatagccga cggcgcaggg cgcgtcatcg ggggccaggt
     8461 tgtgggggca catgcgtccg acctcatcgc cgagatagcc ctggcggtat cgcgccgtct
     8521 caagctggag gatatcagcg agaccatcca cgctcatcca acgttggctg agtctacggc
     8581 agaggcggtc gaggcgactg tggggcaggc gatacacatc tgatgccgtc ttttctctga
     8641 cggcgcccta gaccaatcta catgtttcac gctacccgcg tgctaaaatc atccatgaca
     8701 tcaacgcagt gtacatgtga gacgccgccc cccagcggcg tcgacatgtg ccggagggtg
     8761 acgcggatgg ccgacgagcg cgtgggagtg ttcagaagag agaaaaagcg tgcaggcgac
     8821 gcgtcgggcc caggcaagac gcacggcgac gccgtgaacc cgcggtcgcg caatcgcacg
     8881 ccccggcccc agccccggtc ccagtccaag catcgaaccc agcccccgcc cccgcgcctg
     8941 tcggaccatg ctcgtcttcg ttctcgcctt cgcgttgcta gggccctgac gctcgccgca
     9001 ttcggcgccg caggttttct ggccggagcc gccaggctgt tcggcgccta ttctcctttc
     9061 gggatagtgg ctgtagcggc tctcgcggc
//
LOCUS       DAOTAC010000074         9039 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_6552_length_9039_cov_4.893811, whole genome shotgun sequence.
ACCESSION   DAOTAC010000074 DAOTAC010000000
VERSION     DAOTAC010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 9039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9039
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6552_length_9039_cov_4.893811"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..961
                     /locus_tag="PLM74_04580"
     CDS             <1..961
                     /locus_tag="PLM74_04580"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HON42395.1"
                     /translation="ADTAFSAAYAAQKVGSSVVKIATKQEALVYDFFFMPVVQEREGL
                     GSGVIIDKSGYILTNNHVVEGVTEITVVLVDGRQLPGSIVGTDPYTDLAVIKVEGADL
                     PAAELGNSDKLVIGEPAIAIGNPYGFDHTVTAGVISALNRSVTIDASSGQALEGLIQT
                     DAPINPGNSGGALVTSTGTVIGINTAIIAQAQGIGLAIPINSARTVANEIIQYGKVRR
                     PYVGISRVTEINRYLATRNRLPVDQGLYISSVVRGGPADRAGLGSGDIIIAVGGHDIA
                     TAVDLQKAIYKAAIGDTLEFTVIGTNRRERTARVVLTEAPTSR"
     gene            1035..1514
                     /gene="rlmH"
                     /locus_tag="PLM74_04585"
     CDS             1035..1514
                     /gene="rlmH"
                     /locus_tag="PLM74_04585"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018701810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HON42396.1"
                     /translation="MRIDVVAIGKIKERYIEQAIDDYSRRLSRYCKLSVRTALEEPFS
                     DRDSEARRAQLRVIEGGRLLNLVRPGSYVIASDLAGREVTSEELSALIAGLASSGRSD
                     ITFLIGGPTGYAPEVLTRADDRIRFSRMTFPHQLMRVILLEQIYRAFKIDRNEPYHY"
     gene            1571..2443
                     /locus_tag="PLM74_04590"
     CDS             1571..2443
                     /locus_tag="PLM74_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019543573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HON42397.1"
                     /translation="MHSSEQEVGLKFRIIYYPFMGTDVDPERLLLMARLCYKPLGTDD
                     LYDMVWGSDASAGAKACGGTASGAGDQAVGKFVRTLISSGHLGALEHWYTSFAVEGYS
                     RISSQQNDRHRLMKMFKDPDVPEWVNDPEQGSADLSQLQQSQRYVREHNFHYVVPPSF
                     HQSPEFLQRFRRLQDDIRQLQQYGLNEAKLPAEDVRFGLSNATETRFVITTNARQLRH
                     MFNLRCCQRAQWEIRELFTGLLAEYKRIAPNVFWRAGASCDDLGYCPEGAASCGRAPT
                     LDALLEKSGMKRPD"
     gene            2856..3893
                     /locus_tag="PLM74_04595"
     CDS             2856..3893
                     /locus_tag="PLM74_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HON42398.1"
                     /translation="MAMMSGNSMAAEAKFKVGFIYIGPVGDAGWTTAHDEGRKFMEKE
                     LPWVESVCVESVSEDAMAERMMTQLARQGCKIIFATSFGYMDYVQNVAKNFPNVMFEH
                     CSGFKRDYPNVSTYFGRMYEPRFLSGMVAGMMTKTNVLGYVGAFPIPEVVRGINAFTR
                     GVRFVNPNAKVKVVWTNTWYDPATEKEAAKAALDAGADVIGQHQDTPAPMQAAAERGK
                     YGVSYNSPMSQFAPNAVLTGPVWNWGPYYVKRVKAAKEGTWKSEDYWGGMVDGVVGLA
                     PYGPMVTDKIKKAVADKQAEITAGKFHPFAGPIKDNTGKVKVPAGTTMTDAEMLSFDW
                     FVEGVEGTLPK"
     gene            4000..5523
                     /locus_tag="PLM74_04600"
     CDS             4000..5523
                     /locus_tag="PLM74_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018753040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON42399.1"
                     /translation="MRDITKRFPGVIASNHVNLAVRRGSIHALLGENGAGKSTLMNIL
                     TGLLRPDSGEICVRGERVALRSPASAIALGIGMVHQHFRLVQTFTVAENITLGMSRPR
                     FALDMAPIEREVAKLSSEYGLAVNPHAKIWQLSVGEQQRVEIIKMLYRGVDVLIMDEP
                     TAVLTPQEVRELFETLRRMTADGKTIIFITHKLDEVMEISDRVTVLRAGKDVFSCDTR
                     ETNARELASKMVGREIGQTPERPPVAIGDPVLEVRELTAIGDRGTMVLAGIDLDVRAG
                     EIHGIAGVAGNGQRELAEVITGLRRASAGSVNICGADMTNHTPRSIIEAGVSHIPEDR
                     LGVGLAPNLNITYNAIMKRYRCNEMCRGPIVDATRARTWTDGLVERFNVKTPNLRTAV
                     RLLSGGNSQRLLLARETSMEHKLVVAVHPTRGLDIGATEAVHTILLRERESGVGTLLI
                     SEDLEELIALCDRISVIYEGRIMGTVGRDELNLDEIGMMMAGISAAEKGDGHLEAGA"
     gene            5498..6601
                     /locus_tag="PLM74_04605"
     CDS             5498..6601
                     /locus_tag="PLM74_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HON42400.1"
                     /translation="MDTLKLVLEKRETVSSFYRFVSPLLAIIMALVLGAAFLAASGFS
                     PLKVYYYMWWGAFSDWYGISETLVTSIPIMLCALGVALAAKMLLWNVGAEGQFHMGAF
                     AAAWVALSMPDAPAWQVMPLMVLAGMAAGAIWALLPAVPRAYLGVNEIITTLLLNYVA
                     IYWVEYLVHGPWRDPVNLNFPLSAPFSSAARLPSLFGTRIHLGLVFALVAAGLIWFAL
                     GRTKWGYEVTVIGENPRAARYAGMNIERNIVLTMCLAGALAGLGGMAEVSAIAGRMTR
                     QLSPGYGYSAIIVAWLAKVNPWAVVLVSVLFGGLLVGGYSAQVIGVSAASVNMLQGLM
                     LFCVLATEALGRYRVRAVHAAVRGSGEGASIDG"
     gene            6594..7520
                     /locus_tag="PLM74_04610"
     CDS             6594..7520
                     /locus_tag="PLM74_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HON42401.1"
                     /translation="MADSMFVSILSAAVSSGTPVLYAALGELVCERSGVLNLGVEGMM
                     LVGAVSGFLTAVRTGSLAAGVLAALIAGGLMGLIHALMTITLKVNQVVSGLALTLFGT
                     GLSGYLGKSMIGVPLPVTFSQAPIPGLSSIPLIGTILFKHDLLVYLTYVVVPVLWFYL
                     YRTRPGLHLRSVGESPETADALGVNVFAIRYASVTAGGMLAGLGGAYLSLAYAPSWLE
                     NMTAGRGWIAVALVIFAVWDPFRAMLGAYLFGGIDALGFRIQVLGARIPSFFLKMMPY
                     VFTIVVLIIVTATAARRSSRAPEALGLPYERE"
     gene            7548..8873
                     /locus_tag="PLM74_04615"
     CDS             7548..8873
                     /locus_tag="PLM74_04615"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HON42402.1"
                     /translation="MADNADNKASERRQTIRWNFRISNIDAMLSNVAVGMVTPFIPVF
                     AIALGGSNTEVGLITAIPAFINMLMYLPAASFVERAGSRLKITVRTLLVARLLYLAMA
                     VVPFVPWPQYRAAMLIGILGVQTIPNVIMAVAWTALLGDMFPQEERAHLFAMRNMYSA
                     FVALISSLAAGILLDRIPYPANYMTLFSISFVTGMVALHYCAKLIEGPIRPRPTCRRS
                     LAERLRQPFADPECGRKFRVFAISAALLHLGINIAVPAYSIYHVRTLHLSNSVIGSLS
                     LAAGLTTVLAYPLWGRLSRRAGHSVVYLLSILAYAPFPALYGMHGSVTYLLALQASIG
                     FFNAGFALTILNLTLQHVNPEDSANGIAVFNMLINSTGVLGPPLAAFVVSRYGVTSAF
                     VLSTIVRAAGCIVYIAASGTDDTFVRLHKTLSPSALRRRRLNRKTRGVL"
     gene            8877..>9039
                     /locus_tag="PLM74_04620"
     CDS             8877..>9039
                     /locus_tag="PLM74_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019420265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HON42403.1"
                     /translation="MKLVIAIVRDDYSEILQDELADAGHRYTKLASTGGFLRKGNTTL
                     LVGIDDDQVG"
BASE COUNT         1778 a         2506 c         2843 g         1912 t
ORIGIN      
        1 cgccgacact gccttctctg cagcatatgc ggctcagaag gtgggatcgt ctgtagtcaa
       61 gatcgccacc aaacaggagg cgctggtcta cgacttcttc ttcatgcccg ttgtccagga
      121 gcgtgaaggt ctcggatcag gagtgatcat cgataagtcc gggtacatcc tgaccaacaa
      181 ccatgtggtc gaaggggtta ccgaaatcac tgtggtcctg gtagacggca ggcaactgcc
      241 gggctccatc gtgggcaccg atccctacac cgaccttgcg gtcataaagg tggaaggcgc
      301 ggatcttccc gcagcagagc tcggaaactc cgacaagctg gtcataggcg aacccgcgat
      361 cgctatcggc aacccgtacg gctttgacca caccgttaca gccggggtga tcagcgccct
      421 caaccgttcg gtcacgatcg acgcttcctc cgggcaggcg cttgagggac tgatccagac
      481 ggatgcgcct atcaacccgg gcaattcagg aggggctctg gtcacatcca ccggcacggt
      541 cataggcatc aacaccgcca tcatcgctca ggcacaaggc atcgggctgg cgattccaat
      601 caactcggca cgaactgtgg ccaatgagat catccagtac ggcaaggttc gccgtccgta
      661 cgtgggcata tcgagggtga ctgagatcaa ccgctacctg gccacccgga acagactccc
      721 ggttgaccag gggttgtaca tttcgtctgt cgtgcgcgga ggcccggctg atcgggcagg
      781 gctcggctcc ggcgatatca tcatcgctgt cggcggccat gatatcgcga cagcagtcga
      841 tctgcagaag gccatctaca aggccgccat cggcgatacg ctggagttca cggttatagg
      901 gacgaatcgg cgggagcgta cggcccgggt agtgctgact gaagctccga cgagccgttg
      961 acgggccggc atcggggtga ctccgctcgc ggcgagctcg tgtggtggca agctgctgac
     1021 tggtgactgg tgatatgaga attgacgttg tggccatagg caaaatcaag gagcgttaca
     1081 tcgagcaggc gatagacgac tactccaggc gtctctcccg ctactgcaag ctgagtgtgc
     1141 gcacagcttt ggaggagccg ttcagtgacc gcgacagcga agctaggcgg gcccagctca
     1201 gggtgatcga aggtgggcgc ctgctcaacc tggtgcggcc gggatcatac gtgatcgcgt
     1261 ctgacctggc cggcagggag gtcacatcag aggaactgtc tgcgctcatt gccgggcttg
     1321 cctcatcggg gcgaagcgat atcacgtttc tcataggcgg cccaacaggc tatgcgcccg
     1381 aggtgctgac tcgcgccgac gaccggatcc ggttttcgcg gatgactttt cctcatcagc
     1441 tcatgcgagt catcctgctc gagcagatct atcgcgcatt caagattgac cgcaacgagc
     1501 cttatcacta ctgactcgcg gccgattgca gccgctcagc tacttatact gtcatcaggg
     1561 aggacgtcac atgcacagct cggagcagga agttggcttg aagttcagga taatctacta
     1621 tccattcatg ggcacagacg tagaccccga aaggctgttg ctcatggcga ggctgtgcta
     1681 caaacccctg ggcacggacg acctctacga catggtatgg ggatccgacg cctctgccgg
     1741 ggctaaggct tgtggcggaa ccgcctccgg cgcgggcgat caggctgtgg gcaagttcgt
     1801 gcgcacactg atcagctcgg gccatcttgg ggccctcgaa cattggtaca ctagtttcgc
     1861 tgtggagggc tacagccgga tatccagcca gcagaatgac aggcacaggc tgatgaagat
     1921 gttcaaagat cccgacgtcc ccgagtgggt caacgacccc gagcagggtt cggccgatct
     1981 gtcgcaactg cagcagtctc agcgatatgt tagggaacac aacttccatt atgttgtccc
     2041 gcccagtttc caccagtctc ccgagttctt gcagcgtttt cgcaggctgc aggatgacat
     2101 caggcaattg cagcagtacg gcctgaacga ggccaaactg ccagcggagg atgtgcggtt
     2161 cgggctttcc aatgcgactg aaaccaggtt cgtgataaca accaacgcgc gccagctcag
     2221 gcacatgttc aacctcagat gctgccaacg cgcccagtgg gagatacgcg agctgttcac
     2281 agggttgctt gccgagtaca agaggatcgc cccaaacgta ttctggcggg ccggggcatc
     2341 ctgcgacgac ctgggctatt gccccgaagg cgccgccagc tgcgggcgag cgcccaccct
     2401 cgacgcactt ctcgaaaagt cggggatgaa gagacccgat tagccatccg ccgtttcccg
     2461 aggtttcccg atgctaatcg acattgttcg tggtatcatg ttgtagtccg gaccccgcct
     2521 catgcgcggg gtcgggttcg tgcttctttc gccgcattgc acgccagaca cacacggcga
     2581 gtgctgccgg tgtaccgcgg caggggaggt gtgaaggaaa acacagctcc gtgaagaata
     2641 aggaaagcag tttcgtatgg atctccgatg gttcgtctgc gcaggcgggg cgggtttctg
     2701 cggagccggg ccggtcggcg tggagttgat ctagcattat ctgactcggt acttagttct
     2761 tgcgatacca ctaccagaat tatcacggag ggatgaagat gggcagagcg aggaaggttg
     2821 ttctctcggt cgcgatcctg gtgctgctag gaggaatggc catgatgagt ggcaactcca
     2881 tggcggcgga ggccaagttc aaggttggtt tcatctacat tggccctgtg ggcgacgcgg
     2941 gctggaccac ggcccatgat gagggacgca agttcatgga gaaggagctt ccgtgggtgg
     3001 agagtgtctg cgtcgaatcc gtctccgagg acgcgatggc ggagcgcatg atgacgcagc
     3061 tggccaggca gggctgcaag atcatcttcg cgacaagctt cgggtacatg gactacgttc
     3121 agaacgtcgc caagaacttc cccaatgtca tgttcgagca ttgctccgga ttcaagagag
     3181 actatcccaa cgtgtcaact tactttggcc gcatgtatga gcccaggttc ctttcaggca
     3241 tggtagcagg catgatgacg aagaccaatg tcctgggcta cgtgggcgct ttcccaatcc
     3301 ctgaggtcgt tcggggtatc aacgcgttca ctcgcggcgt caggttcgtc aatccaaacg
     3361 ccaaggtgaa ggtggtatgg acaaacacgt ggtacgaccc ggccactgag aaagaagccg
     3421 ccaaggcagc tctcgacgcc ggcgccgacg tgatcgggca gcatcaggat acccccgctc
     3481 ccatgcaggc tgctgcagag cgcggcaagt acggtgtgag ctacaactcg ccgatgagcc
     3541 agttcgcccc gaacgcggtc ctcaccggcc ccgtatggaa ctggggtccg tactacgtca
     3601 agagagtgaa ggctgctaag gaaggcacct ggaagagcga ggattactgg ggcggcatgg
     3661 tcgatggcgt agtaggccta gcgccgtacg ggccgatggt caccgacaag attaagaagg
     3721 ctgtagcaga caagcaggct gagatcacgg ccggcaagtt ccatccattc gccggaccga
     3781 tcaaggacaa caccggtaag gtgaaagtgc ctgccggcac tactatgacc gatgctgaga
     3841 tgctcagttt cgactggttc gtcgagggcg ttgagggcac gctgcccaaa tagtctggac
     3901 atgtgagcag tacgggacgg ccggcgtcct gcgaaggggc cggccgcccc gtcacagtat
     3961 ggaggattga agttgggcac ggtagattat gccgtagaga tgcgcgacat caccaagagg
     4021 tttcccggtg tgattgccag caatcacgtg aacctcgcag ttcgcagggg cagtattcac
     4081 gcgctcctcg gcgagaacgg cgcaggcaag agtaccctca tgaacatcct taccggcctg
     4141 ctccgaccgg attccggtga gatctgcgtg cgcggggagc gagtcgccct tcgttcgcct
     4201 gcctctgcca ttgctctcgg aataggaatg gtgcatcagc acttccgtct ggttcaaacc
     4261 ttcaccgtgg ctgagaacat caccctgggc atgtcccgcc cacgctttgc gttggacatg
     4321 gcccccatag agcgggaagt tgccaagctc tcgtctgaat acggcctcgc cgtcaacccc
     4381 cacgcgaaga tatggcagct atcagtgggt gagcagcagc gcgttgagat catcaagatg
     4441 ctctaccgcg gtgtagacgt tttgataatg gatgaaccca cagctgttct cacgcctcag
     4501 gaagtcaggg aacttttcga aaccctccgc cgcatgaccg ccgacggcaa gacgatcata
     4561 ttcatcaccc acaagctcga cgaggtgatg gaaatctccg acagagtgac ggtcttgcgc
     4621 gcaggcaagg acgtcttctc gtgtgacact cgcgaaacaa atgccagaga gcttgcgtcc
     4681 aagatggttg ggcgcgagat tgggcagact ccggagcggc caccggtggc gataggagac
     4741 cctgtgctgg aggtgcgtga gctcactgct attggcgacc ggggcaccat ggtgctcgca
     4801 ggaatcgatc tcgacgtgcg cgcaggcgag atacacggca tagccggtgt ggccggtaac
     4861 ggccagcgtg aactggccga ggttattacc gggttgcgcc gggcaagcgc cgggagcgtg
     4921 aacatatgtg gcgccgacat gacgaaccac acccctcgct cgataatcga ggcaggggtg
     4981 agccacatac cggaggaccg ccttggggta ggtttggcgc ctaacctgaa cataacgtac
     5041 aatgcgatca tgaagaggta ccggtgcaac gagatgtgcc gggggccgat tgtcgacgca
     5101 acccgagcca gaacttggac ggacggattg gtggagaggt tcaacgtcaa gaccccgaac
     5161 cttcgcaccg ccgttaggct tttgtcgggc ggcaactcgc agcggctgct gcttgcccgc
     5221 gagacgtcaa tggagcacaa actggtcgtg gcggttcatc ctacccgcgg cctggatata
     5281 ggcgctaccg aggcagtcca caccatactc ctcagggagc gtgagagcgg cgtgggcacg
     5341 ctgttgatat cggaggatct ggaagagttg atcgcgctgt gtgataggat atcggtcata
     5401 tacgaaggac gcattatggg cactgttggc cgtgatgagc tgaacctgga cgaaatcggc
     5461 atgatgatgg ccggcatatc agctgcagag aagggagatg gacaccttga agctggtgct
     5521 tgagaagcgt gagacggtgt cttccttcta caggttcgtc tctcccttgc tcgcgataat
     5581 catggcactc gttttggggg ctgcgttcct tgccgcatcc gggttcagcc cgctcaaagt
     5641 gtattactac atgtggtggg gcgcgttttc cgactggtac ggcatttccg agactttggt
     5701 cacatcgatt ccgataatgc tgtgcgcgct aggtgtggcg ctggctgcca agatgcttct
     5761 gtggaatgtg ggcgctgagg gccagttcca catgggagcg ttcgccgcgg cctgggtggc
     5821 gctttccatg ccggatgcgc cggcgtggca agtgatgccg ctcatggtgt tggccggcat
     5881 ggcggcaggc gccatatggg ctcttctgcc cgcggttccc cgagcgtatc tgggcgtcaa
     5941 cgagatcatt accacacttc tgctgaacta tgtcgcaatt tactgggtcg agtatctggt
     6001 gcacggtccg tggcgggatc cggtgaacct caacttcccc ctgtcggcgc cgttttctag
     6061 cgccgccagg ctgccgtcgc tgttcggcac acgtatccac ctgggcctcg tgttcgcctt
     6121 ggtggcggcg ggcttgatct ggttcgcgtt ggggcgcacc aaatgggggt atgaggttac
     6181 ggtaatcggc gagaaccccc gcgctgcccg gtatgccggc atgaacatcg agcgcaatat
     6241 tgtgttgacg atgtgccttg ccggtgccct ggccggccta ggcggcatgg cggaggtgtc
     6301 ggcgattgca ggccggatga cgcggcaact gtcgccaggc tatggctatt cagcgatcat
     6361 cgtggcgtgg ctggctaagg tgaacccatg ggctgtagtg ctggtgtcgg tgctcttcgg
     6421 cgggctgctc gtagggggat actcggcgca ggtcatcgga gtgagcgccg cgtctgttaa
     6481 tatgctgcag ggcctgatgc tcttctgcgt gctggccacg gaagctctgg gccggtaccg
     6541 cgtaagggct gtacatgctg ctgtccgcgg gagcggggag ggggcgagca ttgatggctg
     6601 attccatgtt tgtgtccatt ctctcggccg cggtcagctc cggcactcct gtgctctacg
     6661 ccgccctggg tgagctcgtg tgcgagcggt cgggcgtgct taacctggga gttgagggca
     6721 tgatgctggt aggcgccgtt agcggattcc tcactgcggt tcgcacaggc agccttgcgg
     6781 cgggggtgct ggcagcgctc atcgccggag ggcttatggg tctgatacat gcgttaatga
     6841 ctatcacgct caaagtcaat caggttgtga gtggcctggc gttgactctt tttggcacgg
     6901 gtctcagcgg ctacctaggt aagtcgatga ttggagtgcc tttgcccgtc acattcagcc
     6961 aggcgcctat cccggggcta tccagcatcc cgttgatagg gacgattctg ttcaagcatg
     7021 acttgctggt gtaccttacg tatgttgttg ttcctgtcct gtggttctat ttgtaccgca
     7081 cccgtccagg cttgcacctt cggtcggtgg gcgaaagccc cgaaaccgcg gacgctcttg
     7141 gagtgaatgt ctttgcaatt aggtacgcga gcgtgacggc aggcggcatg ctggcagggc
     7201 tgggaggggc ctacttatcg ctggcctacg ctcctagctg gctggagaac atgacggccg
     7261 ggcgcggttg gatagcagtg gctctagtaa tattcgcagt atgggatccg ttcagggcca
     7321 tgctcggcgc atacctattt ggaggtattg atgcactcgg tttccgcata caggtgttgg
     7381 gggcgagaat accatcattc ttcctcaaga tgatgcctta cgtattcacg atagtggtgt
     7441 tgatcatcgt gaccgccacc gcggcgcggc gcagttcgag agcgccggag gcgttggggc
     7501 taccgtacga gcgcgaatga ccgggtcgaa cccggagggg tgtttgattg gcagacaacg
     7561 cggacaataa ggcgtcggag cgacgccaaa ccatcagatg gaatttccgg atcagcaata
     7621 tcgacgcgat gctttccaac gtggcagtgg ggatggtcac tcccttcatc cctgtgttcg
     7681 ctattgccct gggcggctca aacacagaag tagggcttat tacggctata cccgctttca
     7741 tcaacatgct gatgtacttg ccggcggcat ctttcgtgga gcgcgccggg agcaggctca
     7801 agatcaccgt gaggacgttg cttgtggcac ggctgctcta cctagccatg gcggtggtcc
     7861 cattcgtgcc gtggccccag taccgcgcgg cgatgcttat tgggattcta ggcgttcaga
     7921 ccatacctaa cgtcatcatg gcggttgcct ggaccgcgtt gctaggcgac atgttccccc
     7981 aggaggagcg ggcgcacctg ttcgccatga ggaacatgta cagcgccttc gtcgcgctga
     8041 tctcgtcgtt ggctgccggc attctgctcg accggatccc ctacccggcc aactacatga
     8101 cgctgttttc catatcgttc gtcacaggga tggtggcgct gcactactgt gcaaagctta
     8161 ttgagggtcc tatcaggccc aggcccacct gtcggaggtc tctcgcggag aggttacggc
     8221 agccatttgc cgacccggag tgcgggcgca agttccgcgt gtttgcgata agcgcggcct
     8281 tgctgcacct gggcatcaac attgcagtcc ctgcctactc catatatcat gttcggacgc
     8341 tccacctgtc caacagtgtc atcgggtcac tgtctttagc ggcagggctc actactgtgc
     8401 tcgcttaccc cctttggggt cgcctgtcgc ggagggccgg gcattcggtg gtgtacctcc
     8461 tgtccatact ggcatatgct ccatttccgg cgctctacgg aatgcacggg tcagtaacat
     8521 atctgctggc actacaggct agcataggtt ttttcaacgc gggttttgcc ctcacgattc
     8581 tcaaccttac actgcaacac gtgaaccccg aagacagtgc caacgggatc gcggtgttca
     8641 acatgttgat taactctacc ggcgtgttgg gtccgccatt ggcggcgttt gtagtgtcaa
     8701 gatacggtgt gacatcggcg ttcgtgttgt caacgatagt tcgcgctgcc gggtgcatcg
     8761 tgtacattgc ggcatcgggt acggacgata cctttgtacg gctgcacaag acgctttcgc
     8821 cgtcggcgtt gcgtcgacga aggctcaacc gaaaaacaag gggggtgctc tagcaggtga
     8881 agcttgtgat agctatagtt agggacgact attctgagat tcttcaggac gaactcgcag
     8941 acgccgggca ccgttacacg aaactagctt ccacaggcgg tttcctcaga aagggcaata
     9001 ccacacttct ggtgggcatc gatgacgacc aggtgggat
//
LOCUS       DAOTAC010000075         8871 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_6705_length_8871_cov_5.211547, whole genome shotgun sequence.
ACCESSION   DAOTAC010000075 DAOTAC010000000
VERSION     DAOTAC010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 8871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8871
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6705_length_8871_cov_5.211547"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="PLM74_04625"
     CDS             complement(<1..698)
                     /locus_tag="PLM74_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004573406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HON42404.1"
                     /translation="MAATVMLILVMMIVPIPPVFLDMLVALNMTFSLGVLLVTMYITE
                     PLQFSVFPSLLLLATLFRLSLNVSATKLILLRGNAGSVIQAFGGFVVGGNYVVGLIVF
                     LILVIIQFVVITTGAGRVAEVAARFTLDAMPGKQMSIDADLNAGMITEEEARRRRLKI
                     QQEADFYGAMDGSSKFVRGDAIAGIIIIVINIVGGIVIGIVQLGLSAGDAFQRFALLT
                     VGGGLVNQIPALLIS"
     gene            complement(768..1847)
                     /gene="flhB"
                     /locus_tag="PLM74_04630"
     CDS             complement(768..1847)
                     /gene="flhB"
                     /locus_tag="PLM74_04630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HON42405.1"
                     /translation="MASHEDRTEQPTQRRREEARKRGQVARSPDLAAAATFTVCLYVL
                     RAALSYSGRIIANLVSHSAQYLGSLGAVDLTEVGAHTLFVNGIGALLLAAAPVALAAA
                     VAGMGVNLAQVGLLVTPQALAPQLNRVDPISGLGRLFSKQALLEMLKAVAKVIVIGYV
                     AYQAIRARVPILLMTAYMDLGEGLRAAGDAVMYVAGRMVIPLVAIGAVDYVVRRRQLE
                     RELRMTKQEVKEELKQQEGDPLVRSRIRQRQRQLAMRRMMEDVKLADVVVTNPTHYAV
                     ALKYDREKMRAPHVVAKGQRIIAQKIREIAMAAGVPVVENPLVAQALFKSVEVGQEIP
                     PDLYRAVAEILAFVYRLGEQRGRRK"
     gene            complement(1880..2734)
                     /gene="fliR"
                     /locus_tag="PLM74_04635"
     CDS             complement(1880..2734)
                     /gene="fliR"
                     /locus_tag="PLM74_04635"
                     /inference="COORDINATES: protein motif:HMM:TIGR01400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HON42406.1"
                     /translation="MDDADNDRVRQAALCDAAGSRQVAMGVFGFTETGFLIFFLVLVR
                     MTGLFILAPVFAHASIPAQVKAWFAFALTLVIYPVVGQTSISVPGDVAQFGLVILREL
                     LVGFVVGYVALLVFAAAQYAGELIDTQIGFGMANLVDPSFGAQVTVVGQFHFLTATMV
                     YLAVNGHHFLVGALVRSYDVVPIGQAGLSPDLFDSIVLRFGELFKIAMRMALPAAMCL
                     LITEMGLGIAARAVPQMNVLMVGFPLKICVGLAVLVLAMPGFVGYVRVVLGRLQEAVV
                     LIIGAMRS"
     gene            complement(2665..2895)
                     /gene="fliQ"
                     /locus_tag="PLM74_04640"
     CDS             complement(2665..2895)
                     /gene="fliQ"
                     /locus_tag="PLM74_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018313572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HON42407.1"
                     /translation="MAVAFKVSAPVLLAALTVGVIVSVLQAATQIQEMTLTFVPKVVA
                     IAAVLLLAGPWMMRTMTGFARQLFAMLPALAK"
     gene            complement(2948..3748)
                     /gene="fliP"
                     /locus_tag="PLM74_04645"
     CDS             complement(2948..3748)
                     /gene="fliP"
                     /locus_tag="PLM74_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015839103.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HON42408.1"
                     /translation="MSKARTRMEKAICRASAAAALLVAAVGLALVWSAVAHATPLRIP
                     GIQLGVNPAAGPREVAGTLQILALLTVLSLAPAILLMMTSFTRIVIVLSFARSAMGTQ
                     QVPPNQVIIGLALFITLFVMMPVVAEINETAWQPYMSGRISQEVALERGAKPIRDFML
                     KQTREQDLSLFVDLSGRPEPARPDDVPTYVIIPAFMISELKSAFQMGFVIYIPFLVID
                     MLVASVLMSMGMMMLPPVLISLPFKILLFVLVDGWGLVVKSLVQSFAG"
     gene            complement(3745..4083)
                     /locus_tag="PLM74_04650"
     CDS             complement(3745..4083)
                     /locus_tag="PLM74_04650"
                     /inference="COORDINATES: protein motif:HMM:NF016250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HON42409.1"
                     /translation="MERSMAELLLRATAALAIVSGLIYLSGHLLKKAQAAGLIRAVAG
                     ERRIKLLETARLGPDSYLHLVQVDDGTVLVATGRGGVAVSASASPSASASVLEAVSTT
                     DSDAREVSAL"
     gene            complement(4090..4335)
                     /gene="fliN"
                     /locus_tag="PLM74_04655"
                     /pseudo
     CDS             complement(4090..4335)
                     /gene="fliN"
                     /locus_tag="PLM74_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288426.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
     gene            complement(4620..5609)
                     /gene="fliM"
                     /locus_tag="PLM74_04660"
     CDS             complement(4620..5609)
                     /gene="fliM"
                     /locus_tag="PLM74_04660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HON42410.1"
                     /translation="MAEVLTQEEINALLAQFAASDEESAAPAGATPEGRAYRLYDFKR
                     PNRFSREQLRTIHMLHETFARHLSTTLSAHLRTPVKVILGEVQQQIYDEYLSSLKAPE
                     VMYLFTPEPLEGQAAIEFSPYIVFIMIDRLMGGPGRVRGRIRELTEIEENLARSIVNR
                     TMEDLREAWDPMVKIRPHIDRYESNPQFAQVVSPNDTVCLITFQIKVGEASGSMSICM
                     PHSTLQPVLQRLSTQHWYQKGNRVNPERSSQAIATGLERVKVPVAAVLGSASIDMRDM
                     LDLQVGDIIRLNERASSSMTLVIGKTPKFHVIPGLRGKNHAVQIVRPLDSNEK"
     gene            complement(5652..6107)
                     /locus_tag="PLM74_04665"
     CDS             complement(5652..6107)
                     /locus_tag="PLM74_04665"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HON42411.1"
                     /translation="MRKRRLIVVLAVLLVAAAVIVGYVVISGRGPAGEASADEDGANG
                     VVQPNLAMGPGYTFDTFIVNVAGTGGRRYLKCTITVEFDKNAGVTEAGTKVPLLRDAM
                     IDILSSKTLDDLEPGPLRDALRGELVSTISSVITKARVMRVFFNEFVVQ"
     gene            complement(6148..6909)
                     /locus_tag="PLM74_04670"
     CDS             complement(6148..6909)
                     /locus_tag="PLM74_04670"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HON42412.1"
                     /translation="MSRRKKAEESGGQAPAWMTTYSDLMSLLLCFFVLLFAFSTVDAA
                     KFREALISLQGALGVLNQGPAMSNPADRPIPPENPSQVSEDSGEGAAEGVENLLVLKG
                     QLQQYLEERGLEGRVHLELNRRGLLIRFTDTVLFDLGRARLRLDSRQLLKDISKFIGP
                     IRNEVMVEGHTDNIPLRPDAQFETNWELSTARATTVVRYFIEELGLAPQRFSAAGYGE
                     YRYVMPNTSDGNRAMNRRVDILIVAMDGTDIAQGM"
     gene            complement(6906..7751)
                     /locus_tag="PLM74_04675"
     CDS             complement(6906..7751)
                     /locus_tag="PLM74_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="motility protein A"
                     /protein_id="HON42413.1"
                     /translation="MDIATIGGFVLGTAFILIALMNGSAGLRPFWDFDSVLITIGGTI
                     AAVMISFPFSRIKDLPRITAIAFREREADPVAIIDSMVDLAEKARREGLLALEDSVNR
                     AEDEFLKRGIQLIVDGTDPELVRDILETDLTYIEDRHKIGKSMYDAMTAYAPAFGMIG
                     TLIGLVQMLRTLDKPSTIGPAMSVALITTFYGAVIANLITGPIANKLALKSGAEIRLR
                     EMMLEGILSIQSGENPRIVEEKLMGFLAPKDRMRAQDQAEARKAGRRARTEMAAETGG
                     GAVAR"
     gene            complement(7764..7979)
                     /locus_tag="PLM74_04680"
     CDS             complement(7764..7979)
                     /locus_tag="PLM74_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019152781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HON42414.1"
                     /translation="MIRVTKLNGSPIHVNAELIETIASTPDTVITLTNGTKLIVAEPE
                     EKVVQEIIRYHRLVHRRAVIVRNWADK"
     gene            complement(8044..>8871)
                     /locus_tag="PLM74_04685"
     CDS             complement(8044..>8871)
                     /locus_tag="PLM74_04685"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HON42415.1"
                     /translation="YRPTGNAAFAGNLDPTSSSHVMDPVTVYDSEGQPHSLVVTFTKT
                     VDDEWDWTTTLDGGTPVTGGHLVFDTDGAITSGQTGTVVVDPAANVSVDFGSLAQVTG
                     STAVTGTQDGIPVNAISFNPGTGAAPLSITPDFSTITQVGDESSVSWSTQNGYPAGSL
                     ETFNIDDRGIITGVYSNGQYKVLGQMVLASFANSEGLMRQSNNMYAETTNSGAPQLGE
                     ATKSGRGAVLGTTLEMSNVDLAREFTDMIVTQRGFQANSRVITAADEMLQELLALKR"
BASE COUNT         1838 a         2863 c         2359 g         1811 t
ORIGIN      
        1 gagatgagca gggcggggat ctggttcacc aacccgccgc ccacggtgag cagcgcgaat
       61 ctctgaaacg catcgcccgc gctcaagcca agctgcacta tgccgatgac gatgccgccc
      121 acgatgttga tgactatgat tatgattccg gctattgcgt cgccccggac aaacttgctg
      181 gagccgtcca tggcgccgta gaagtcggcc tcctgctgta tcttgagcct gcgccgacgt
      241 gcctcttcct cagtaatcat gcccgcgttc aggtccgcgt cgatactcat ctgtttgccg
      301 ggcatagcgt ccagggtgaa cctggcggct acttccgcca ctcggccggc gccggtggtg
      361 atcacgacga actggatgat cacgagtatc aggaacacga tcagccctac tacatagttg
      421 cctccgacca cgaaaccacc gaacgcctgg atcacggatc cggcgtttcc ccgcagcaag
      481 atcagcttgg tggccgacac gttcaggctc aaccggaaca gcgtggcaag cagcagcaat
      541 gaagggaaga ccgagaactg cagcggctca gtgatgtaca tggttactag cagcaccccg
      601 agggagaacg tcatgttcaa ggccacaagc atgtccagga agacaggggg tatcggcacg
      661 atcatcatga cgaggatgag catgaccgtg gcggccatga acaggtcccc gcctttgaga
      721 tacctgatta cggcggacct cgcctctgaa cggatatcgg ccactgccta cttcctcctc
      781 ccccgctgct ctcccagcct atacacgaac gccagtatct cggccacggc ccgatacaga
      841 tccggcggta tctcctgccc aacctctacc gacttgaata aggcctgcgc cacaaggggg
      901 ttctcgacta caggcactcc tgccgccatc gcaatctcgc gtatcttctg tgcgatgatg
      961 cgttgccctt tcgccaccac gtgaggcgct ctcatcttct cccgatcgta cttgagcgcc
     1021 acagcgtagt gcgtggggtt agttaccaca acatcagcga gcttgacatc ttccatcatg
     1081 cgcctcatgg ccaactgcct ctggcgctgc ctgatccgcg atctcaccag aggatcgcct
     1141 tcctgctgct tcagctcttc cttgacctct tgcttggtca tgcgcagttc gcgttcgagt
     1201 tgcctcctcc ttacgacata gtccacggcg cctatcgcca caagcgggat gaccatccgg
     1261 cccgccacat acataaccgc gtcgcccgca gcgcgcaggc cttcgcccaa atccatgtag
     1321 gctgtcatca gcagtatcgg caccctcgcc ctgatcgcct ggtaggcaac atagccgatc
     1381 acaataacct tggcaaccgc tttgagcatc tccagcagag cctgtttcga gaataggcgt
     1441 cccagtccgg aaatgggatc tactctgttg agctggggcg ccaacgcctg cggcgttaca
     1501 agcagaccga cctgcgccag gttgaccccc atgccggcca cagccgccgc caatgccacc
     1561 ggcgctgcag ccagaagcag tgcaccgatc ccattcacaa acaacgtgtg tgctccgacc
     1621 tccgtaaggt ccactgcgcc aaggctaccc aagtactgag cgctatgcga aacgagattg
     1681 gcgatgatcc ggcccgagta tgatagcgca gccctcagca catacaggca taccgtgaaa
     1741 gtcgcggcgg cggcgagatc agggcttcga gctacctgcc cgcgctttcg ggcttcctcc
     1801 cgccgtcgtt gcgttggttg ctctgtcctg tcctcatgcg atgccaccgg tcctcacctc
     1861 cccgggcggc ttcacccaat cagcttcgca tggccccaat gatcagtacg acagcctcct
     1921 gcagtcgccc cagcacaacc cggacatatc ccacgaaccc cggcatggcc agaaccagaa
     1981 cggccagccc cacacatatc ttcaggggaa accccaccat cagcacgttc atctgcggca
     2041 cggcccgcgc cgcaattccc aggcccatct cagtaatgag caggcacatc gcagccggca
     2101 aagccatacg catggcgatc ttgaacagct ctccgaacct cagcacgata gagtcgaaca
     2161 ggtccggtga gaggccggcc tggccaatgg gaaccacatc atagcttcgc actagcgcgc
     2221 ccacgaggaa atggtgcccg ttgaccgcca ggtagaccat ggtcgccgtc aggaagtgaa
     2281 actggcctac cactgtgacc tgcgctccga aagacgggtc caccaagttg gccatgccga
     2341 accctatctg cgtatcgatg agttctcctg catactgcgc cgctgcaaac accaacaatg
     2401 ccacatagcc caccacaaac cctactagca gctcacgcaa tatcaccagg ccaaactgag
     2461 caacgtcgcc gggcaccgat atcgaagtct ggcccaccac agggtatatc acaagggtca
     2521 aggcaaacgc gaaccacgcc ttcacctgcg ccggaatgct cgcatgcgcg aacaccgggg
     2581 ccaggatgaa cagaccggtc atcctcacca gtaccaggaa gaatatgagg aatcccgtct
     2641 ccgtgaaccc gaacacaccc atggctactt ggcgagagcc ggcagcatcg caaagagctg
     2701 cctggcgaac ccggtcattg tccgcatcat ccatggccct gcaagcagca gcaccgccgc
     2761 tattgccacg accttcggca caaacgtcag ggtcatctcc tgtatctgcg tggcagcctg
     2821 gagcaccgac acaatgactc ccacagtcag cgccgcgagg agcacgggag cgctcacctt
     2881 gaaggccacc gccattgcct gcgatgccac agtcgccacc gtagcttcgt tcacttaccg
     2941 tacctcctca tcccgcgaag ctctgaacca aagacttcac gaccagtccc catccatcta
     3001 ccagcacgaa caggagtatc ttgaagggca gagatatcag cacgggcggc agcatcatca
     3061 tgcccataga catcagcaca ctcgcgacta acatgtcgat gaccagaaac ggtatgtata
     3121 tcacgaaccc catctgaaag gccgatttca gctcgctgat catgaatgcg gggattatga
     3181 cgtaagtggg cacatcgtcg gggcgcgcgg gctcaggtcg cccagataag tctacgaaaa
     3241 gcgataagtc ctgctcgcga gtttgtttga gcatgaaatc gcggatgggt ttggccccgc
     3301 gttccagcgc cacttcctga ctgatccgac cggacatgta cggctgccat gctgtctcat
     3361 tgatctcggc gactaccggc atcattacga agagtgtaat aaagagagcc agcccaataa
     3421 tgacctggtt tggcggcact tgctgtgtgc ccatggcgct gcgcgcaaac gacaggacga
     3481 tgactattcg cgtaaaagag gtcatcatca gtagaatcgc cggagccagc gaaagcaccg
     3541 tcaagagcgc caatatctga agcgtacctg ccacttcccg cggaccagca gccgggttaa
     3601 cgcccagctg aatgccggga atgcgcaggg gagtcgcgtg tgccacagcc gaccacacca
     3661 gtgccagccc taccgcggcg accagcagtg cagcggcggc agatgcccgg catatcgcct
     3721 tctccatgcg cgtgcgcgct ttgctcatag ggcgctcacc tcccgggcat ccgagtccgt
     3781 cgtggaaact gcctcgagca cggacgcgga cgccgacgga gacgcagatg ccgacactgc
     3841 cacaccgcca cggccagtcg ccaccagcac cgtcccgtcg tctacctgca caagatggag
     3901 atacgagtca ggcccgagtc gcgcagtctc cagcagcttg atgcggcgtt cgcccgccac
     3961 agccctaatc agtcccgcgg cctgcgcctt tttgagcaga tgccccgaaa gatagatgag
     4021 gccagagact atcgcgaggg cggcagtcgc ccggagcaac agctccgcca tgcttctctc
     4081 catctcgccc tacactgcct tctctggatt gctcatgaac tcgacgatcc tcacgccgaa
     4141 gttctcctcg atgacaacaa cctcgccccg cgcgatgaga cgaccgttaa cgaggatgtc
     4201 gacgttttcc ccggcggcct tctccagttc gatgacggat ccgggcccaa gcgccatgat
     4261 gtctcggacc agcatggtag tccggccaag ctccactatg accggcagag gcacgtccat
     4321 gagcaggtcg agattgcccg tggcagcttt gggtttggcc tcgtcaaact gcatgaactg
     4381 cacctgctgg atactcggca tgtctgctga tgcctgatcg gaacgtcgca ccacagatgg
     4441 cgccgcggca gcgggttcct cgtgcgctgc ggccgcagca tgtactgcag acgtcgacgg
     4501 gcccgcgaga tccggaacct cgggtgtcaa gtcgaaactg ggaacagcgt cgccatcggt
     4561 ctgaacgtca tcccagaatg tctcgtttcc agtagtctca tcgtgcaatg ttattcgcct
     4621 catttctcat tggagtccaa gggccgaact atctgaacgg cgtgattctt ccctctaagg
     4681 ccagggatga catggaactt gggagtcttg cctattacca acgtcatgct gctcgaggct
     4741 cgttcattca gtctgatgat gtcccctacc tgcaggtcga gcatgtcacg catgtcgatc
     4801 gaggcgctgc ccagcactgc cgcgactggg accttcactc gctcgagccc tgttgctatg
     4861 gcttgagacg acctctccgg gtttacccgg tttcccttct ggtaccagtg ctgtgtgctc
     4921 agccgctgca gcaccggctg gagtgtggaa tggggcatgc atatgctcat gctgcccgat
     4981 gcctccccca cctttatctg aaatgtaata agacatacgg tatcgttggg cgataccacc
     5041 tgcgcgaact ggggattcga ttcgtaccta tcgatgtgcg gacgtatctt gaccataggg
     5101 tcccaagcct ctctcaagtc ctccatagta cggttgacta tgctgcgcgc cagattctct
     5161 tcgatttccg tcagttccct gatgcgccca cgtactcgtc ccggcccgcc catcagcctg
     5221 tcaatcatta tgaacacaat gtatgggcta aactcgatcg ctgcctgtcc ttccaggggt
     5281 tcgggagtga aaaggtacat gacctccggc gccttaagcg aactcaggta ctcatcgtat
     5341 atctgctgct gtacctcacc gagaatcacc ttgacaggcg ttcgaaggtg agctgacaga
     5401 gtagtcgaca gatggcgcgc gaaggtctca tggagcatgt gaatagtcct gagctgctcg
     5461 cgggagaacc tgttcgggcg cttgaagtca tatagcctgt aggctcgccc ttccggcgtc
     5521 gcgcctgcag gcgcggcaga ctcctcatca gacgctgcaa actgagcaag caacgcgttg
     5581 atctcttcct gagtcaatac ctcagccaca cgcattcact cccggtcctg ccggcgcaag
     5641 cagtgcccgc actactggac cacaaattca ttgaagaaca ctcgcatgac cctggccttc
     5701 gtgatcacgg aactgatagt ggacactagc tcccccctga gcgcgtcacg cagcggtccg
     5761 ggttccagat catccagggt cttagatgaa agaatatcga tcatggcgtc acgcaacagc
     5821 ggtaccttgg ttccagcttc agtgacacct gcattcttgt cgaactccac tgtgatagta
     5881 catttcaagt accggcgtcc gcccgtaccc gcgacgttga caatgaatgt atcgaaggtg
     5941 tatccagggc ccatggcgag gttgggctgc accacaccat tcgcaccgtc ctcgtcggca
     6001 gatgcttcgc cggccggtcc gcgcccggaa atcaccacat agccgacaat aaccgcagct
     6061 gccaccagca aaaccgccag gactactatc agtcgccgtt ttcgcatccg cccgatcccc
     6121 ctgaccgtca ccgtctgggt ttcagcacta catgccctga gcgatgtctg tgccgtccat
     6181 ggcaactatg agaatatcca cacgcctgtt cattgcccga ttcccatcgg aagtgttcgg
     6241 catcacgtag cggtattctc cgtatcctgc cgcagaaaac cgctgcgggg cgagccccag
     6301 ttcttcgatg aaataccgta cgaccgtggt ggcccgcgcc gtcgatagct cccaattcgt
     6361 ttcgaactga gcgtcagggc gaagtggaat gttgtcggta tgcccttcca ccatcacttc
     6421 gttacggata ggacctatga atttggagat gtctttcaag agctgcctcg agtcgaggcg
     6481 caaccgcgcc cggccaaggt caaacaagac cgtatccgtg aaccggatga gcagcccacg
     6541 cctgttcagc tcaagatgta ctctgccttc gagcccgcgc tcctccagat actgctgcaa
     6601 ctgacccttg aggaccaaca ggttctccac accctcagct gcgccttcac cactgtcttc
     6661 gcttacctgc gaaggattct caggaggtat cggacggtcg gccgggttcg acatggcagg
     6721 cccctggttc agcactccca gggcgccttg cagagatatc agtgcctcgc gaaactttgc
     6781 ggcgtcaacc gttgagaacg caaacagcag cacgaagaag cagagcaaga gcgacatcag
     6841 gtcgctgtac gtagtcatcc aggccggagc ttgccctcct gattcctctg ccttcttcct
     6901 cctgctcatc tcgcaacagc tccccctccg gtctccgcag ccatctcggt tctggcccgc
     6961 ctgccagcct tgcgagcctc cgcctgatcc tgtgcgcgca tgcggtcctt cggcgcgagg
     7021 aaccccatga gtttctcctc cacgatacgc ggattttcgc ctgattgtat ggacaagatg
     7081 ccctccagca tcatctcgcg gagcctgatc tcggcgccac tttttaaggc cagcttgttc
     7141 gcgatgggac cagtgatcag gttggcgatc actgctccgt aaaaggtcgt gatcaatgca
     7201 actgacatag caggcccgat ggtgctgggc ttgtccaacg ttcgcagcat ctgcaccagg
     7261 cctataagcg tcccgatcat cccgaaggcc ggcgcgtagg cggtcatggc atcgtacatg
     7321 ctcttcccga tcttgtgccg gtcctcaatg taggtcaggt cagtctctag aatgtcgcgc
     7381 actagttccg gatcggtacc atccacgatc agctgaatgc cgcgtttgag aaactcgtcc
     7441 tccgccctgt tcacactgtc ttccagcgcc agcaagcctt cacggcgggc cttctcggca
     7501 aggtccacca tactatcgat gatagcaaca gggtcggcct ctctctcacg gaaggcaata
     7561 gcggtgattc tgggcaggtc tttgatcctt gagaaaggga acgatatcat caccgcggct
     7621 atcgttccgc cgatggttat gagtacagag tcgaaatccc agaacggcct caggcctgcg
     7681 cttccgttca tgagggctat cagtatgaat gctgtgccca gtacgaatcc acctattgtg
     7741 gcgatgtcca tgctaatccc tcatcacttg tccgcccagt tcctcactat cactgcccgc
     7801 ctatgcacga ggcggtgata gcggattatc tcctggacga ccttctcctc tggctctgcg
     7861 acgatgagct tcgtgccatt agtcagagtg atcaccgtgt cgggggtgga agctatcgtc
     7921 tcaattagct ccgcgttaac atgtatcggc gaaccgttga gtttcgttac tcttatcacc
     7981 gagcagcgcc ccctttcgcg ggtctagccc gggcggagcg gatcgccccg cccgggaagc
     8041 aggttacctc ttcagcgcca agagttcttg cagcatctca tcagcagccg tgatcaccct
     8101 ggaattggcc tgaaaccccc tctgcgtgac gatcatgtcg gtgaactccc gcgccaagtc
     8161 cacgttggac atctcgagcg tcgtgccgag cactgcgccc ctgccgctct tcgttgcctc
     8221 tccaagctgc ggagcgccag agttagtggt ctctgcatac atgttgttgg attgtctcat
     8281 caggccctcg gagttggcaa acgacgccag caccatctgg cccagcacct tgtactgccc
     8341 attggaatag accccggtga tgatcccccg atcgtcgatg ttgaacgtct ccagcgagcc
     8401 ggccggatag ccgttctgcg tgctccagct taccgacgat tcgtcgccga cctgggtgat
     8461 ggtgctgaag tctggagtga tgctgagcgg agcggctcct gtgcctgggt tgaacgatat
     8521 ggcattgacg gggatgccgt cctgagtgcc agtgaccgca gttgatccgg tcacctgggc
     8581 cagactgccg aagtccacac tgacgtttgc agcaggatcg acgacgacgg taccagtctg
     8641 tccgctggtt atggctccat ccgtgtcgaa caccaggtgg ccgccggtaa ccggcgtgcc
     8701 gccatccagt gttgttgtcc agtcccattc gtcgtccaca gtcttcgtga acgtcactac
     8761 gagagagtgg ggttgcccct ccgagtcgta cactgtcacc ggatccatga catgcgaaga
     8821 tgacgtgggg tcgagattgc cggcgaacgc cgcgttgccc gtgggcctgt a
//
LOCUS       DAOTAC010000076         8697 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_6898_length_8697_cov_4.437167, whole genome shotgun sequence.
ACCESSION   DAOTAC010000076 DAOTAC010000000
VERSION     DAOTAC010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 8697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8697
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6898_length_8697_cov_4.437167"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="PLM74_04690"
     CDS             complement(<1..741)
                     /locus_tag="PLM74_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HON42416.1"
                     /translation="MIQMFEQNALSDVKHVVGVMSGKGGVGKSSVTALGAVALARRGH
                     TVGIMDADITGPSIPKMFGVHGRLDASETGIFPAQTSKLGIRVVSLNLMLEQEDEPVV
                     WRGPLLMGMIRRFWSEVNWGNIDYMLIDLPPGTGDVPLSVMQSIPLDGLIAVSSPQDV
                     AAMIVRKTINMARMMDKPVIGLVENMSYITCPHCGEAVYPFGRPSGEEQAASLGIPFI
                     GGMPIDPELSAVADRGEVELYEHPIMETL"
     gene            982..1809
                     /locus_tag="PLM74_04695"
     CDS             982..1809
                     /locus_tag="PLM74_04695"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HON42417.1"
                     /translation="MYAESTIYAKEGMVVLMMTALGAIDINSDVGESFGAWRMGYDEH
                     TLASVTSANIACGFHAGDPITMRETVHIAAKLNVSCGAHPGYPDLVGFGRRDMAMSPD
                     EAVADLAYQVGALWAIAHAEGVQLRHVKPHGALYNRAARDHALALALAHAVRSLDLGL
                     VLVGLAGSAMEQAAGETGIAFAREAFADRAYNSDGSLVSRSRPGAVIEDPAVVARRAV
                     SIARDGSIETYDGGRISLGAHTICVHGDTRQAADTARAVKEAIAAAGIRIEPLWKIV"
     gene            1964..2794
                     /locus_tag="PLM74_04700"
     CDS             1964..2794
                     /locus_tag="PLM74_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydro-lyase"
                     /protein_id="HON42418.1"
                     /translation="MTEITQGWEGASPREFRSAVRDGKWASPTAGVCMGYAQANLAIL
                     PAKQAMDFLVFANRNPKPCPIIEVLDPGCPEPVYSAPGADIRTDLPKYRVYRQGRLEE
                     ERSDIIDLWKDDFVSFLIGCSFSFEKALLDAGVPVRHIELGRNVPMYMTNIQCRPAGI
                     FHGPMVVSMRPIPGGAVARAVEVTAAMHAVHGAPVHIGSPEAIGISHINHPDFGDSVP
                     IYEGEVPVFWACGVTPQAAALAAKPEIMITHSPGHMFITDVRDRDLLDFMSGLGGAAK
                     "
     gene            2791..3540
                     /gene="pxpB"
                     /locus_tag="PLM74_04705"
     CDS             2791..3540
                     /gene="pxpB"
                     /locus_tag="PLM74_04705"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013467315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HON42419.1"
                     /translation="MNVHEDAPRFLWAGDSAVVTQFGNDISPLLASRVHRAASALEGA
                     NLPWLVEVLPSYRSVLAEFDPRLISPTAACEVIQGIMKTIVTSDGHTSDEHTSDGLGG
                     ESGRAEGDIVTIPVCYGGQFGTDLPDVAAHTGLTPDEVIALHSEPLYLVYMIGFTLGF
                     PYLGGMDPRIATPRLATPRTVVPAGSVGIAGNQTGVYPSASPGGWRLIGRTPLRLADP
                     SADPPCLLRAGNRIRFVPIDEPTYYSMEGNQ"
     gene            3537..4655
                     /locus_tag="PLM74_04710"
     CDS             3537..4655
                     /locus_tag="PLM74_04710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HON42420.1"
                     /translation="MMANVLEVASPGPLSMLQDLGRPGWMKYGVPPSGALDQEALAVA
                     NLLAGNPEGAVGIEITYGNFTARALDRTVLACAGAWAPVNVDGMPVSPWAAFSVMPGQ
                     VVSIGVPTWGVRVYLAVRGGIAAPLVMGSASTYVRGALGGIDGRGRALRTGDLIATPD
                     QSASARASTSPVGDVTARAVAALAKRQAAFAIAHSPALARASLSQPNSTSASTSASPG
                     RSIAIRVVMGPQDDHFTDAGRQTFVEHEFTLTNKADRMGLRFDGPAIQHVHGADIISD
                     GMPPGAIQVPGDGVPIIMLADRQTTGGYPKIACVARADLDLAAHLRPGDRVRFIPLSA
                     EDAHEAFKQEQAASRAPGRALRIRIDGVEHVVTLFELQ"
     gene            4689..5714
                     /locus_tag="PLM74_04715"
     CDS             4689..5714
                     /locus_tag="PLM74_04715"
                     /inference="COORDINATES: protein motif:HMM:NF025694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4392 domain-containing protein"
                     /protein_id="HON42421.1"
                     /translation="MTKGTRINDDALSIVAETIDRLISMDFRDRGITGRLYAAARDHV
                     GAPLAHAAALAISQALDPPAQLAQAAASARKGAIICTGFMIRSAGQPETDGLTGAALL
                     ARGLATAFGIDPVLATDDTAVGPLAAACQAAGLPTEAVIVPFPAGRAPAAIRPLAADL
                     LSSVKPGVVISIERPGANSAGVHHNSRGQSISDIVAPLDELFTQARRAGVPTVAIGDL
                     GNELGMGVIANTLRALNPLGSRCACPCESGIACDTPADVTVVGAISDDAAYGILACLA
                     ALRGDINILPTPDMLQAVLRAAVAAGAVDGLTGKSEYSIDMLGEAIHCALIGLMGAVL
                     ACAGYGE"
     gene            complement(5767..7089)
                     /locus_tag="PLM74_04720"
     CDS             complement(5767..7089)
                     /locus_tag="PLM74_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HON42422.1"
                     /translation="MSKMNLTSKRIPTSKRNPISKMNLVSKTGLALKASFELTVLGGM
                     TKVNDYPEITTDGRILMGPGPSGVHPRVLRALGAPILGHLDPEFITIMNRTKDLLAWV
                     FETKNEFTIPISGTGSAGMETALVNFIEPGDRVLVCVNGLFGTRMADIVERCGGELEV
                     IEGEWGKIIEPDAVEKSLKARPAKIVAIVHAETSTGVLQPLEPVAQIAHRYGAMLVVD
                     AVTSLGGTPVRVDETGIDVCYSGTQKCLSCPPGLAPITVGRLAAERLAARKSKVQSWY
                     LDLTMLHSYWGKERFYHHTAPINMIYALYESLRIIREEGLERRYERHSLHSRAFGAGV
                     TAMGLRLFAQEGFRAPMLMAVAIPEGVDDAAVRRYLLSTYRLEIGGGLGPLKGRIWRV
                     GLMGHSCSSENVVLLLAGLEGALVQQGMPVERGAGVEAAREVYKASGM"
     gene            7541..8593
                     /locus_tag="PLM74_04725"
     CDS             7541..8593
                     /locus_tag="PLM74_04725"
                     /inference="COORDINATES: protein motif:HMM:NF013408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HON42423.1"
                     /translation="MGRLDAAYPMLFAADLKEKVWGGRRLESWFENMPPGPAPIGEAW
                     VLSAHPNGPTRITNGRLTGRTLVELCPEFPLLIKVLSCEADLSVQVHPPDHYPRLRAG
                     ESGKSEVWLVLEAAPGASIIYGLAEGVTPELLRRALTTALTTAMTPATSEVAYTTVHA
                     ASATKSSAADIMNCFRRVRVAAGDLIPVPPGTVHALGAGLVVAEVQQSSDTTYRLWDY
                     GRLGIDGKPRELHIEQALKVASYSRPPAITHPFAFELEANTPQRMCSFANFDIWRSTC
                     SGQWHRNASPNAFRAILALSGRGALQWNDDQSPVSPHQSIALRPGSCVLIPASCPSYT
                     LQCDHGQLGLLEVTPA"
BASE COUNT         1703 a         2823 c         2535 g         1636 t
ORIGIN      
        1 caacgtctcc atgatcgggt gttcgtacag ctccacttcc ccgcgatcgg ccacggccga
       61 aagctccggg tcaatgggca ttccgccgat aaacgggatt ccgaggctcg cggcttgctc
      121 ttcgccggag ggtctgccaa atgggtacac tgcctcgccg cagtgggggc aggtgatgta
      181 gctcatgttc tccaccaggc cgatcacggg cttgtccatc atccgggcca tgttaatggt
      241 tttacggact atcatggccg caacgtcctg aggtgatgac actgcgatca gcccatcgag
      301 gggtatggac tgcatgaccg agagcggaac atcgcccgtt cccggcggca ggtctatcag
      361 catgtaatcg atgtttcccc agttgacctc ggaccaaaac cgccttatca tccccatcag
      421 aagcggaccg cgccacacca caggctcgtc ctcctgctcc agcatgaggt tgagagaaac
      481 gaccctaatc ccgagcttgc tcgtctgggc cgggaagatt ccggtctccg acgcgtcaag
      541 ccggccatgg acaccaaaca tcttgggaat gctcgggccg gtgatgtcgg cgtccattat
      601 gccgactgta tggcctcgcc gtgccaacgc cactgcacca agcgccgtca ccgatgactt
      661 gcccacaccg cccttgccgc tcattacccc gacaacgtgt ttcacatctg acagcgcatt
      721 ctgctcaaac atctgtatca tacgaattgt gctccccctg tgattggtaa tgctatccac
      781 acctatgcta gaggagtttc ggacatatgt caataacgta ccctcggctg acctgccgcg
      841 gcctgcacac ccggacctgg cactgccatt atcattctcg ccacgcctcc cagctatttc
      901 gctctcgccc gtcccaattc atcatctcga tttctgcatt ttatcaggca acgactgaga
      961 ttttaggtag gatacaagtc tatgtacgca gaatctacaa tatatgcgaa ggaaggaatg
     1021 gtggttctga tgatgacggc cctgggggca atcgatatca acagcgatgt cggcgaatcc
     1081 ttcggcgcgt ggcggatggg ctatgatgag cacacactgg cctcggtgac cagtgccaac
     1141 atcgcatgcg gtttccatgc tggcgacccc atcaccatgc gcgagacggt tcacatagcc
     1201 gctaagctga acgtatcgtg cggcgcacat ccaggctatc cggacctggt gggattcggg
     1261 cggcgcgaca tggctatgtc cccggatgag gccgtcgcag atctggcata tcaagtcgga
     1321 gcactatggg caatcgcaca tgccgaaggg gtgcagctca ggcacgtcaa accccatggt
     1381 gctctctaca atcgcgccgc ccgcgatcac gcgctggccc tcgccctggc ccatgccgtc
     1441 cgctcgctcg acctcgggct cgtgctcgtg gggttggcag gctctgccat ggagcaggcc
     1501 gccggcgaga cgggcatcgc attcgcccga gaggcattcg ccgacagggc ctacaactcc
     1561 gatgggagcc tggtgagccg gtcgcgccca ggggccgtca tcgaggaccc tgccgtagta
     1621 gcacggcgcg cagtgtctat cgcgcgcgac ggttccatag aaacgtatga cggcggccga
     1681 atcagcctgg gcgcccacac tatctgcgtg cacggcgaca ccaggcaagc tgccgacacc
     1741 gcccgagcag tcaaggaggc cattgcagcg gcaggtatcc gaatcgagcc gctgtggaag
     1801 atcgtatagc aggcgtgggt gtgggtgcag gtgcaggtgc aggcgcaggt gcgggtgcgg
     1861 atgcgggtat ggctgtaggt gtagccgtaa gcgcaatccc gccgcccgta gccgggccgc
     1921 tgacagcgga gtagagcaaa tcaggactga aggtgatccg gatatgaccg agataaccca
     1981 aggctgggaa ggcgcaagcc cgcgcgaatt ccgctcagca gtcagagacg gcaagtgggc
     2041 ctccccaact gcaggtgtat gcatgggata tgcccaggcc aatctggcga tactcccggc
     2101 caaacaggcc atggacttcc tggtgttcgc caacagaaac cccaagccgt gcccgatcat
     2161 cgaagtactc gaccctggct gcccggagcc ggtatacagt gcgccggggg ccgacatccg
     2221 cacagatctg cccaagtatc gtgtgtaccg tcaagggcgg ctggaggagg aacgctcgga
     2281 tatcatagac ctctggaagg acgacttcgt ctcattcctc atcggctgca gtttctcgtt
     2341 tgagaaagcc ctgctggatg cgggcgttcc ggtcaggcac atagaactag gccgtaacgt
     2401 gcccatgtac atgacgaaca tccaatgcag gcctgccggg atcttccatg ggcccatggt
     2461 cgtgagcatg cgcccgatcc ccggcggcgc cgtcgcccgg gccgtggaag tcacggcagc
     2521 catgcatgcc gtgcatggcg cgccggtcca cattgggtcg cccgaggcta tcggcatatc
     2581 ccatatcaac caccccgact tcggcgattc agtccccata tacgaaggcg aagtgccggt
     2641 gttctgggcg tgcggcgtta caccgcaggc cgcggccttg gccgcgaaac cggagataat
     2701 gattacgcat tcgcccggac acatgttcat caccgacgtc cgcgatcgtg acctgctcga
     2761 cttcatgtcc ggtctgggag gcgccgcaaa gtgaacgtcc acgaagacgc tccccgattt
     2821 ctgtgggccg gcgacagcgc tgtggtcaca caattcggca acgacatctc gcccctgcta
     2881 gcctcgcggg tccaccgcgc cgccagtgcc ctggagggcg ccaacctccc ctggctagtt
     2941 gaggtgctgc cgtcctacag gtcggttctg gccgaattcg acccgcgcct gatctcgccc
     3001 accgcagcct gtgaggttat tcagggcatc atgaagacga tcgtcacttc cgacgggcac
     3061 acgtcagacg aacacacctc cgacgggctg gggggcgaat cgggcagagc cgaaggcgat
     3121 atcgttacca tccccgtgtg ctacgggggg cagttcggga cggacctacc tgacgtagca
     3181 gcgcacacag gccttacgcc tgacgaagtg atagcattgc acagcgagcc gctctatctc
     3241 gtctacatga tcggcttcac gctaggcttc ccgtatctgg gcgggatgga tccccgtatc
     3301 gcaacaccac gtctggctac ccctcgcaca gtcgtgcccg ccggatcggt aggcatcgca
     3361 ggcaaccaga ccggggtcta cccgtcagca agtcctggcg gttggcgcct gatagggcgc
     3421 acgcccttgc gcctggcgga tccgtccgct gacccaccgt gcctgctccg cgcaggcaat
     3481 cgcatacgat tcgtccctat agacgagcca acatactact ccatggaggg caaccaatga
     3541 tggccaatgt gcttgaagtg gcaagcccgg gcccgctctc catgcttcag gacctgggca
     3601 gaccgggatg gatgaaatac ggagtccccc cttccggcgc tctggaccag gaagcccttg
     3661 cagtcgccaa tctgctggcg ggaaacccgg aaggcgcagt ggggattgag ataacgtacg
     3721 gcaacttcac agctcgagcc ctcgatcgga ccgtgctggc gtgcgcaggc gcctgggccc
     3781 cagtgaacgt ggacggcatg cccgtgtccc cctgggcagc cttcagtgtc atgccagggc
     3841 aagtcgtaag catcggcgtg ccgacctggg gagtgcgagt gtatctcgcg gtgcgcggag
     3901 gaatcgcggc ccctctggtg atgggcagtg catccacata cgtccgggga gcactggggg
     3961 gcatcgatgg ccgggggcgc gccctgcgta caggcgacct catagctaca ccagaccagt
     4021 ctgcttccgc gagggcctca acctcccccg tgggcgacgt aactgcgcgg gcagtcgcag
     4081 ctctcgccaa gcgacaggct gcgttcgcga ttgcgcattc accggcgcta gcccgagcgt
     4141 ctctttcgca gccgaattcc acgtcggctt ccacgtcggc ttcccccggg cgttccattg
     4201 ctatacgtgt agtaatgggc ccgcaggacg atcactttac agacgctggc cggcagacgt
     4261 ttgtcgaaca cgagttcact ctgaccaaca aggcggaccg aatgggattg cgattcgacg
     4321 gcccggccat ccagcacgtc cacggggccg acatcatctc cgacggcatg ccccctggcg
     4381 ccattcaggt tcctggagac ggcgtgccca tcataatgct ggccgacagg cagaccaccg
     4441 gcgggtatcc caagatagcc tgcgtggccc gcgctgacct ggaccttgcg gcgcatctgc
     4501 gcccaggcga ccgggtccgg ttcattccgt tgtcagctga agacgcacat gaagcattca
     4561 agcaggagca ggccgcgtcc cgggcccccg gccgtgcgct caggatcagg atcgacggcg
     4621 tcgagcatgt ggtcacgctc tttgaactcc agtaggctcg cgcaccaaga cggaaggacg
     4681 gatcgaagtt gacgaagggc acacggatca acgacgacgc gctgagtatc gtagcagaga
     4741 ccatcgaccg cctgatctcc atggactttc gtgaccgtgg gatcacaggc cggctgtatg
     4801 ccgccgcccg agaccacgta ggcgcgccgc ttgcgcatgc cgccgccctc gccatcagcc
     4861 aagcgctcga ccctccggcg cagctggctc aagctgcggc ctctgcgcgc aagggcgcca
     4921 tcatatgcac aggcttcatg atccgctcag caggccaacc tgaaacagac ggcctgaccg
     4981 gcgccgcgct gctcgcgcga gggctggcca ctgccttcgg aatcgaccct gtactggcta
     5041 ccgatgatac agccgtgggc ccactggccg ctgcctgcca agctgcaggt ctgcctacag
     5101 aggcggtgat cgtaccattc cctgcgggcc gagcccccgc cgcgatccgg ccactggcag
     5161 ccgatttgct gtcatcagtc aagccggggg tagtcatctc aatcgaacgg ccaggcgcca
     5221 acagcgcagg cgtccatcac aactcccgcg ggcagtccat atccgacatc gtcgcccccc
     5281 tggatgagct gttcacacag gctcgcagag ccggagtgcc gaccgtggcc atcggcgatt
     5341 taggcaatga gctgggcatg ggggttatcg ctaacacctt gcgggcgctg aacccactgg
     5401 gctcgcggtg cgcctgccca tgcgagtctg gcatagcgtg cgacacgcct gccgacgtca
     5461 ctgtggtcgg agcgatatca gacgacgccg cgtacggcat acttgcttgc ctcgctgcgc
     5521 tgcgcggcga tatcaacatc ctgcccacac ccgacatgct ccaggcagtc ctgagggcgg
     5581 ccgtcgccgc cggggccgtg gacggattga ccggcaagtc agaatactcc atcgatatgt
     5641 tgggcgaagc catccattgc gcgcttatag gactgatggg cgccgtgctg gcatgcgcag
     5701 ggtacgggga atgaggtggc tgtctcccca accccgaccc cgacgccgac gcccgaaccc
     5761 cgaaccctac attcccgacg ccttgtagac ctctcttgca gcctcaaccc ctgcgccccg
     5821 ctccacaggc atgccctgct ggacgagggc gccttcgagg ccggcgagga gcagaacaac
     5881 attctcgctg ctacacgagt gccccatcag gcccacgcgc catatgcgtc cctttagcgg
     5941 acccagtccg ccgcctatct ccaggcggta ggtggacagc aggtagcgcc gaacggccgc
     6001 atcgtccacg ccctcaggaa tcgcaaccgc catcagcatc ggagccctaa atccctcctg
     6061 agcgaacaga cgcaggccca ttgccgtcac accagcgcca aatgccctgc tatggagcga
     6121 gtgacgctcg tagcgccgct caaggccttc ctcgcggatg atgcggaggc tttcatacag
     6181 agcgtagatc atgttgatcg gggcagtatg atggtagaac cgctccttac cccagtagct
     6241 gtggagcatg gtcaggtcca gataccagct ctgaaccttg gacttcctgg cggccaacct
     6301 ctcggctgcc agccggccta cggtgatggg cgctagccca ggcgggcacg acaggcattt
     6361 ctgagtgccg ctgtagcaga cgtcgatacc ggtttcgtcg acacgcacag gcgttccgcc
     6421 caggctggtt acggcatcta ccacaagcat agcgccatat ctatgggcga tctgggccac
     6481 cggctcgaga ggttgcagca cacctgtgga cgtttcggcg tgcacaatcg ctactatctt
     6541 cgcaggccgt gccttgagag acttctcgac cgcgtcgggc tcgataatct tcccccactc
     6601 gccttcgatt acctcgagct caccgccaca cctctcgacg atgtctgcca tcctcgtgcc
     6661 gaataggcca ttcacacaca ctagcaccct atcgccaggc tcgatgaagt tcaccaaggc
     6721 cgtctccata cccgcactgc cggtgccgga tatcgggatc gtaaactcgt ttttcgtctc
     6781 gaacacccat gcaagcaggt ccttagtcct gttcataatc gtgatgaact ccgggtccag
     6841 atgccccaga atcggcgccc ccagggctct cagcaccctc gggtgcacac cgctggggcc
     6901 agggcccatg agtattcgtc catctgtagt tatctccgga tagtcattca ccttggtcat
     6961 ccctccgagt acggtcaatt cgaagcttgc cttcaatgcc aagcctgtct tcgacaccag
     7021 gttcatcttc gatatcggat ttcgcttcga cgtcggaatc ctcttcgacg tcaggttcat
     7081 cttcgacact gactactcga atccttgcat agctttcccc aacagacaat cgccggttcc
     7141 gtcaagctca agtcaccttc tacggccaac cggaccccga gtgaaacgaa cccatctcca
     7201 tggatacgtg cgttttcgct cggagacgaa gcgtaacttg gcagcagaac gagcgcacga
     7261 tggggtatga tgcggatggg aaaacctagg tgtcgctttt ccgaacacgt ttttggagat
     7321 gtgtcgccaa aacgaacaat gagtgatgca taatgagacg cataggcgca tgaagcggta
     7381 tgtttgaatc ttcagactag tccatcctcc aattatggta tgttgtgtat tgtgtatgtc
     7441 tgagaaatgc ggcaacgagg acgggcaatc gactaggcca tgtgttgacc ccagcaggct
     7501 tttatcggta atatgagact gacattgcga ggtgttgttc ttgggcagac ttgatgcggc
     7561 ttatccgatg ctttttgcgg cagacctcaa agaaaaagtg tggggcggtc gtcgcctgga
     7621 atcgtggttt gagaacatgc cgcccgggcc cgctcccata ggcgaagcct gggttttgtc
     7681 cgcgcatcca aacggcccta caaggataac caatgggcgc ctgacaggac gaaccctggt
     7741 cgagctctgc cctgagttcc ccctgctcat taaggtgcta agctgcgaag ccgacctgtc
     7801 agttcaggtt cacccccctg accactatcc acgcctgcgc gccggtgaat cgggaaaatc
     7861 cgaggtctgg ctcgtgctgg aagctgcacc cggagcttcg atcatatatg gactggccga
     7921 aggcgtgacg ccggagttgc taaggagggc actcaccacg gcgttgacca cggccatgac
     7981 cccagccaca tccgaggtcg catacacaac cgtacacgcg gcctcagcca caaagagcag
     8041 tgccgccgac atcatgaatt gcttccggcg ggtccgtgtc gccgccggcg acttgatacc
     8101 tgtgccaccg ggcaccgtgc acgcgctcgg cgccgggctg gtggtagcgg aagtgcagca
     8161 atcttcggat acgacatacc gattgtggga ctacggtcga ctcggaatag acggcaagcc
     8221 acgcgaactc cacatcgaac aggcgctaaa ggtcgcgagc tactcgcggc cccccgccat
     8281 cactcatccg ttcgcgttcg agctcgaggc caatactccg cagcgtatgt gcagtttcgc
     8341 gaacttcgac atctggcgga gcacgtgctc aggccagtgg catcgcaatg cttcgcccaa
     8401 cgctttcagg gctatcctcg cgctctccgg ccgcggcgcc cttcaatgga acgatgatca
     8461 gagccccgtc agcccccatc aatcgatcgc cctgcgcccc ggctcgtgcg tgctcatacc
     8521 cgcctcatgc cccagctaca ccctacaatg cgaccacggc cagctggggt tgcttgaggt
     8581 gacaccggca taggcatgca cagcacgcgc gccatcaacg gtcgcaggtc gcatccatga
     8641 tcgccgccag cccggggccg tatggcgccc gcactatccc ccgctccgtc acgatag
//
LOCUS       DAOTAC010000077         8550 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_7062_length_8550_cov_4.668040, whole genome shotgun sequence.
ACCESSION   DAOTAC010000077 DAOTAC010000000
VERSION     DAOTAC010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 8550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8550
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7062_length_8550_cov_4.668040"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1603
                     /gene="nuoF"
                     /locus_tag="PLM74_04730"
     CDS             <1..1603
                     /gene="nuoF"
                     /locus_tag="PLM74_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HON42424.1"
                     /translation="VVYVHVKPEDVAEIVEQTLRRGKVVERLVYRDPNTGRVYPKERD
                     IPFYASQTKVALANCGVINPDDIGEYIARDGYQALARAVLDLTPDETIEEVLESGLRG
                     RGGGGFPTGRKWMFARLAQGDQKYVVCNADEGDPGAFMDRCIMEGDPHSVLEGLAIAA
                     SAIGADRGYIYVRAEYPLAVARMKNAVRQARECGLLGADILGSDFSFDVTIKEGAGAF
                     VCGEETALLASIEGKRGMPRPRPPFPANEGLFGKPTIINNVETLATVPAIVRRGPTWF
                     RAIGTDTSPGTKTFAVTGQVANTGLVEIPMGATLRDVVFKIGGGVIGGKKFKAAQIGG
                     PSGGCLPEQLLDMPLDYDSLRKAGAMVGSGGLVILDEGTCMVEVARFFMHFTQKESCG
                     KCVPCREGTKRMLEIMEKIVAGRATLEDLDALEQIAWVVKESSLCALGKTAPNPVLTT
                     LQYFRDEYLAHIVDKKCPAGVCSAFKAYVIHSELCKGCGKCRRVCPAGAITGEPKHPP
                     VIDTSKCVKCGACVEACAFGAISLG"
     gene            1635..3671
                     /locus_tag="PLM74_04735"
     CDS             1635..3671
                     /locus_tag="PLM74_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HON42425.1"
                     /translation="MPVQQRVVIDGLSVPIEGERNVLELVRKAGIDLPTFCYHSELSV
                     YGACRMCVVEIDKMGIVASCTVEPQPGMVVRTNTEQLRTIRRTILELLLANHDRDCTT
                     CDKNGACRLQELARRFGVRDVRFGEGDLSLPLDDSTPALVRDPNKCILCGDCVRVCGE
                     VQKIGVLDFSYRGSEAMVGPAFGKSLADVDCVQCGQCASVCPTGAITIHSDIDRVWKA
                     IHDPNIKVVAQVAPAIRVALGEEFGHEPGWAEPGKVVAALKSIGVDMVFDTCFTADLT
                     VVEETAEFLERLSSGSRERLPQFTSCCPAWVKYVEQYHPEYLPNLSSCKSPQQMFGAV
                     ARRFLARELGIEPESLYVVSVMPCTAKKFEAARTEFAVDGRPDVDAVLTTQELARMIR
                     ETGVDFDKLPIAPFDLPLGVSTGAGMIFGNSGGVAEAVLRAAAELLAPEKTSGIFRTR
                     LVFEEVRGLEGVKEATVNVAGNEVRMAVVSSLGAAAEVLGRIESGEAVYDIVEVMSCP
                     GGCIGGAGQPSPSHLEVRKERTKALYDVDTMQMLRRSHDNPYIKALYDRYLGDVGGEE
                     AHSALHTSYGMRRRIAGEDIRFSAEEVDGRLSVGVCVGTCCYLHGSYDLLRGLMDRVK
                     QYELTDKVDLHATFCFENCSHSPNIEIDGNMRNGVTLEEIDRVFQEEILARLVE"
     gene            3691..4350
                     /locus_tag="PLM74_04740"
     CDS             3691..4350
                     /locus_tag="PLM74_04740"
                     /inference="COORDINATES: protein motif:HMM:NF003994.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HON42426.1"
                     /translation="MPEREVRISRAMLARLPFYYRALAAHARAGNTKISSAQLAASLG
                     MDSARVRRDLSSIGEFGRPGIGYNVPELPEALEEMLGLRNRTEAVLVGVGRLGAALFG
                     YPGFEAYGLRIVGLFDSDPAKIGTELHGAMIRSISEIPHVVRRLSIQMGIITTPPAGA
                     QEIADMLVDAGVVAIWNFAPISLSVPPGVMVRDEDLAAGLSYLSRHLSGGTNAARPAQ
                     E"
     gene            4417..5298
                     /locus_tag="PLM74_04745"
     CDS             4417..5298
                     /locus_tag="PLM74_04745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HON42427.1"
                     /translation="MRVAVILNPNARHGRAGEDEAEAVRTLQDLGIDFDLIKTSRPGD
                     AVSMARRAAEQGYDVVCAMGGDGTVNEVINGIAGTGAALAVIPAGTGNDFAGALGVPK
                     GDVAAACRILARGHLRPMDLCRVNDRYFASSFGAGFDARVTKAANERFKRLGGIWTYI
                     FAVMSVIWSFRPGPTRIVADGREISKSPLLVAVTNWKSYGGGMYICPGAEIDDGLFDV
                     CIVDNVPILKFLYSFPRVIRGTHVTMPEVEIFRARSVHISCSRTETYHVDGEVFESHE
                     MELTLVPAGINVVIGEV"
     gene            5302..7260
                     /locus_tag="PLM74_04750"
     CDS             5302..7260
                     /locus_tag="PLM74_04750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HON42428.1"
                     /translation="MRLLLAAIVGYCIGSVSSAVIVGRAVGGIDVRKYGSGTAGGANV
                     GRLLGVRYGALVACCDIAKGMTATAIGRAIGGTTGQMASGAAAVIGHILPLWFGFAGG
                     KAIATFFGSCVLTAPVAVLPAGALWLAVYLGLRSVAQASAIASACLPLIAWVCRLSWR
                     HILYLGVMGAAICLRHIPDARGPGQGRHPRRPGGRSGRPTKPGARRLMVIVVASGVGL
                     CLTMLPRVASSGEPIRVRVGLVGLPARVALGSSSSRASLRIAGSLEMPVALFEPGEIG
                     QIVCVAPDGTRTCLESPVAVEILALDPSVARDGSSPGSSPGSAMPLTWVGQPADVYRG
                     QFELACGKSGEMHVINILDLEDYVAGVVPYEIGAGAPLEALKAQAVVARTEALALAGR
                     HAQEPFDACSSTHCQVYRGAAAELDRPDVRRAVDSTRGEVLFYNGKPAPGAKYHACCG
                     GITESAGALWRFDVPYMIGVGCGVSRPEAAPDSLTAIRDEEAVRAAIAAPDPRDFCHG
                     SNGYRWKVEYTAGQLATVLAPVIGRLEPPNARIIGVTVVDRTPRGSAVRVRVTTTEGE
                     YEIAGEYAIRSALGGTVTIKSGVFVVDTEGDPPSLFVFSGAGYGHGVGMCQYGARGMA
                     RAGYSYRAILGHYYPGTTVEPILRSAGN"
     gene            7270..7863
                     /locus_tag="PLM74_04755"
     CDS             7270..7863
                     /locus_tag="PLM74_04755"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HON42429.1"
                     /translation="MIVDDRIYERAQSGDRSAINELFEAYADTAARMARQMAGACQDL
                     EDIVQEALLRAFRSIGSFRWDSSFQTWLYRITANVCRDFARKSAVCRCVSLDALCSED
                     EGATDAACQAMSTVAAQPEAEAIRREQREAIVRAVAALPRLDRAVFVMREIGSLSYKD
                     IANSLGCSIESVRSRLARARRQVRTNIQAFLPDAARP"
     gene            7912..>8550
                     /locus_tag="PLM74_04760"
     CDS             7912..>8550
                     /locus_tag="PLM74_04760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HON42430.1"
                     /translation="MSLVRVVTDSSNGIPPEFVEKYGIEIVPIQVQFGADSYKEGRDL
                     SLAEFYRRLDEPVLPTTSQPSPGDFVETYKGLVGKCDEIISIHLTSKGSGTCQSARLA
                     SQMIEGVRITIVDTLTASMGTGLIVMAAAEAAYHGCGRDEIMRIIQSGMDTTSVFVAL
                     PTLKYLRRSGRVPQVQAWVAGLLSIHPILGVRDGVVEAVERVRTFRGCVRRVV"
BASE COUNT         1635 a         2309 c         2900 g         1706 t
ORIGIN      
        1 tgtggtgtat gttcatgtga agcctgagga tgtagccgaa atcgtagagc agacactccg
       61 aagaggcaag gtcgttgaaa gactggtata tcgcgatccc aacacaggcc gggtatatcc
      121 taaggagcgc gacattccgt tctatgcaag tcagaccaag gttgctttgg caaattgcgg
      181 ggtcatcaac cccgatgaca taggagagta catcgcccgc gatggctatc aggctctggc
      241 ccgcgcagta ctggatctga cgccggacga gaccatcgag gaggtgctgg agagcggact
      301 ccgtgggcga gggggcggag gtttccccac aggccgcaaa tggatgttcg cgcgtttggc
      361 ccagggcgat cagaagtatg tcgtgtgcaa cgcggacgaa ggcgaccccg gggcattcat
      421 ggatcgctgc attatggaag gcgatcccca cagcgttctg gaggggttgg ccatcgcggc
      481 cagcgccatc ggcgcggatc gcggctacat ctatgtgcgg gcagaatacc cgctggctgt
      541 ggcccgcatg aagaatgccg tccggcaagc cagagaatgc ggcctattgg gtgccgatat
      601 tctgggctct gatttctcgt tcgatgtgac tatcaaggag ggcgcgggtg ccttcgtgtg
      661 cggcgaggag accgctcttc tcgcatcgat cgaaggcaag cgcggcatgc ctcgcccgcg
      721 gccgccgttc ccggcgaatg agggtttgtt cgggaagcct acgatcatca acaatgtaga
      781 gacgttggca acagtcccgg cgatagtccg ccggggcccg acatggttcc gcgccatcgg
      841 aacggacaca agcccgggca cgaagacgtt tgccgtcacc ggtcaggtgg ccaatactgg
      901 tcttgtagag atccccatgg gggcgacctt acgggacgtc gtcttcaaga tcggcggagg
      961 cgtgatcggc gggaagaagt tcaaagcggc acagataggc gggccctcag gcggatgcct
     1021 gccggagcag cttctcgata tgccgctgga ctatgactct ctgaggaaag ccggcgccat
     1081 ggtcggctcc ggcggactcg tcatacttga cgagggaacc tgcatggttg aggtagctcg
     1141 cttcttcatg catttcactc agaaggagtc gtgcggcaag tgtgttccgt gccgcgaggg
     1201 caccaagcgc atgctggaga tcatggagaa gatcgttgcc gggcgtgcaa ctcttgagga
     1261 cctggacgct ctggagcaga tcgcctgggt tgtgaaggag agttcgctgt gcgctctggg
     1321 caagacggcg ccgaatccgg tcctcacgac gctgcagtat ttccgagacg agtacctggc
     1381 gcacatcgta gacaagaagt gccctgccgg ggtatgcagc gctttcaagg cgtacgttat
     1441 ccactccgag ctctgcaagg gctgtggcaa gtgccgcaga gtgtgtcctg caggggcgat
     1501 caccggcgag ccgaagcatc ctcctgtgat agacacttcc aaatgcgtga agtgcggggc
     1561 gtgtgtcgag gcatgtgcct tcggcgccat ttcgctagga tagatcggaa tagaggggaa
     1621 agagagggcg cgaaatgcct gtgcaacaga gagttgtcat agatggattg tcagttccga
     1681 tagagggcga gcgcaatgtg ctggaactgg tgcgcaaggc aggaatagac ctgcccacat
     1741 tctgctacca ttcggaactt tcggtatatg gggcctgcag gatgtgcgtt gtcgagatcg
     1801 acaagatggg gatagtcgca tcctgcactg tagagcccca gcctggaatg gtggttcgca
     1861 cgaacacgga gcaactgcgc accataaggc gcacgattct cgagcttctt ctggccaacc
     1921 acgaccgtga ttgcactacg tgcgacaaga acggcgcatg caggcttcag gagcttgcga
     1981 gacggttcgg ggtccgggat gtgagatttg gcgaggggga cctgagtcta cctctcgacg
     2041 actccacgcc tgcgctggtt cgcgacccca acaagtgcat actctgcgga gactgtgtgc
     2101 gagtatgcgg cgaagtgcag aagatcggag tgctcgattt ctcgtaccgc ggttccgagg
     2161 ctatggtcgg ccctgcgttc gggaagagcc tggccgatgt ggactgcgtg cagtgcgggc
     2221 agtgtgcgag cgtctgcccc accggcgcga tcacgataca ctccgacatc gacagagtat
     2281 ggaaggctat tcacgatccg aacatcaagg tagtggcgca ggtggcgcca gcgatccgtg
     2341 tggccttggg cgaggagttc gggcatgagc caggctgggc tgagcccggc aaggtcgtgg
     2401 cggcactcaa gagcatcggt gtggacatgg tcttcgatac atgtttcact gccgacctta
     2461 cggtagtgga ggagacggct gagttcctgg aaagactatc gtcaggcagc cgggaacgac
     2521 tgccccagtt cacgtcgtgt tgcccggcat gggtcaagta cgtggagcag taccatccgg
     2581 agtacctccc gaacctgtcg tcatgcaagt cgcctcagca gatgttcggc gcggtggccc
     2641 gtcgattcct cgcacgcgag ctgggcatcg agcctgaatc gctgtatgtg gtgtcggtca
     2701 tgccatgtac ggccaagaaa tttgaggctg cccgcacgga gttcgccgtg gatggcaggc
     2761 ccgatgtgga cgctgttctg acaacgcagg agttggcgcg catgatacgt gaaacaggcg
     2821 tcgacttcga caagctaccc atagccccct tcgacctgcc cctcggggtg tctaccgggg
     2881 ccggcatgat cttcgggaac agcggcgggg ttgcagaagc ggtgctgcgg gcggcggctg
     2941 agctcctggc gccggagaag acctcgggta ttttccggac ccggctggtc ttcgaggagg
     3001 tgcgcgggct cgaaggtgtg aaagaggcga ctgtgaacgt cgccggcaat gaagtccgga
     3061 tggcggtcgt gagctctttg ggcgccgcgg ccgaggtgct cgggagaatc gaatcgggcg
     3121 aggcagtgta tgacatcgta gaagtcatgt cgtgcccggg cggatgcata ggaggcgcag
     3181 gccagcccag tccgtcgcac ctcgaggtcc gcaaggagag gactaaggcc ctctatgatg
     3241 tggacacaat gcagatgctg cggaggtcgc atgacaaccc gtacatcaag gcgctatacg
     3301 accggtacct tggcgatgtc ggcggcgagg aggcccattc ggccctccac acgtcttacg
     3361 gaatgcggag gcgaatagct ggcgaggata tcagattctc cgccgaagaa gtcgacggcc
     3421 ggctgagtgt tggagtgtgc gtgggcacct gctgctatct gcacggatcg tatgacctgc
     3481 tgcgagggct gatggacagg gtcaagcagt atgagctcac tgacaaggtg gatttgcatg
     3541 ccaccttctg ctttgaaaac tgcagtcaca gcccgaacat cgagatagac ggcaatatgc
     3601 ggaatggcgt gactttggag gagatcgata gggtgttcca ggaggaaatc ctcgcacgtc
     3661 ttgtagaata agagacgcgg aggtggtcgt atgccggagc gcgaggtcag gatatccaga
     3721 gcgatgcttg caaggctgcc gttctactat cgggcgctgg cggcgcatgc gcgcgccggg
     3781 aacactaaga tctcttcggc gcagcttgcg gcgagtcttg gcatggattc cgcccgggta
     3841 cgacgcgact tgtcgtccat cggtgagttt ggccgccctg gtatagggta caatgttccg
     3901 gaattgcctg aggcactcga ggagatgctg ggcctcagga atcgcaccga ggcggtcctg
     3961 gttggcgtgg gccgcctcgg cgccgctctt ttcgggtacc ccggcttcga ggcgtatggc
     4021 ctgcgtatcg tgggtctgtt cgattcggat cccgcaaaga tcggcacaga gcttcatggt
     4081 gccatgatcc gcagcatctc ggagattcct catgtggtcc ggcgcctgtc cattcagatg
     4141 gggataatca cgacgcctcc ggccggtgct caggagatag ccgatatgct ggtggacgcg
     4201 ggcgtcgtag ccatctggaa cttcgccccc atcagcctgt cggtcccgcc tggagtgatg
     4261 gttcgcgacg aggacttggc agcgggcctt tcttaccttt cgcgccatct atcgggcggg
     4321 acgaatgcgg cacggcctgc gcaggaatga cgctgcaaat gttgaattca gaatagtatt
     4381 tgcagtggct taccttgtac aatagggggc agggaattgc gtgtcgcagt catattgaac
     4441 cccaatgcga ggcatgggcg agcaggagaa gacgaagcag aggcggtacg gactcttcaa
     4501 gacctgggca tcgacttcga cttgatcaag acgtctcgcc cgggggatgc cgtttccatg
     4561 gcgcggaggg ctgccgaaca gggctatgat gttgtgtgcg ccatgggcgg agatggcaca
     4621 gtcaacgaag tgatcaacgg aatagcaggc acaggggcgg cgctcgcggt gatacccgcg
     4681 ggcaccggga acgactttgc aggtgctttg ggcgttccga agggcgatgt ggcggctgcg
     4741 tgccggattc tggcacgggg ccacttgcgt cctatggacc tatgtcgggt gaacgacagg
     4801 tactttgcgt ccagtttcgg ggcgggtttc gatgcccgcg tgactaaagc cgcgaacgag
     4861 cgcttcaagc gcctgggcgg gatatggacg tacatattcg cagtgatgag cgtcatatgg
     4921 tctttccggc cggggccgac gcgcatcgtg gcggacggcc gcgagatctc caagtctcct
     4981 ctgcttgtcg ccgtcactaa ctggaaaagc tacggtggcg gaatgtacat atgcccgggc
     5041 gccgagatcg acgacgggct atttgacgtg tgcattgtcg acaacgttcc catactcaag
     5101 tttctataca gcttcccccg ggtgatccga ggcacgcatg tgaccatgcc ggaggtggag
     5161 atattccggg cacggtcggt gcacatctcg tgttcgcgga cggagacata tcatgtggac
     5221 ggcgaggtgt tcgaatccca cgagatggaa ttgacgctcg ttcccgcagg catcaacgtg
     5281 gtcatcgggg aggtgtagcc tatgcggctc cttctggccg ctatcgttgg ctactgcata
     5341 ggctcggtat cctccgcggt gatcgtgggc agagcggtgg gaggcattga cgtccgcaaa
     5401 tacgggtcgg gcactgccgg cggcgccaat gtcggaaggc tgctgggcgt aaggtacggt
     5461 gcactggtag cctgctgcga tatagccaag ggaatgaccg ccacagcaat tgggcgggca
     5521 atagggggaa cgaccgggca gatggcgtcc ggggcagccg cggtgatcgg gcacatcttg
     5581 ccgctgtggt ttggattcgc cggcggaaaa gcgatagcca cgttctttgg ctcgtgcgtg
     5641 ctcactgcgc cggttgcggt gctgcctgca ggcgcgctgt ggcttgcggt gtatcttggt
     5701 ctgcgctcgg tcgcgcaggc atctgcgata gcttctgcgt gcctacccct catagcctgg
     5761 gtgtgccgcc tctcgtggcg gcacatactg tacctggggg tgatgggtgc ggctatctgc
     5821 ctgcggcata tcccggacgc gcgcgggccg gggcagggga ggcatcccag acgaccgggg
     5881 gggagatcgg ggaggccgac gaaacccgga gcgcgtaggc tgatggtgat agtggttgcc
     5941 tccggcgtcg ggctttgcct cacaatgctc cctcgagtcg ccagttccgg cgagcccatc
     6001 cgcgtgaggg ttgggcttgt gggccttcca gcccgggtgg cgctgggctc gagttcgagc
     6061 cgggcttcgc tccgcatagc gggcagcttg gagatgcctg tggctttgtt tgagcccggt
     6121 gagatcgggc agatagtatg cgtggctcca gatggaacac gaacctgtct tgaaagcccg
     6181 gtagctgtgg aaatcctcgc tcttgatccg agcgtcgcac gcgacggttc gtcgcctggt
     6241 tcgtcgcctg gttccgctat gccgctgaca tgggtgggtc agcccgccga cgtgtaccgc
     6301 gggcagtttg aactggcgtg cggcaagtcc ggagagatgc atgtgatcaa catccttgac
     6361 ctggaggact atgtcgcagg cgtggtgccg tacgagatcg gcgcgggcgc gcctctcgag
     6421 gccctgaagg cgcaggcagt tgtggcgcga accgaggccc tagcgcttgc ggggcgccat
     6481 gcccaagagc ctttcgacgc ttgctcgtcc acgcattgcc aagtctatcg gggagccgct
     6541 gcggaactcg accggcctga tgtaaggcgc gctgtggact cgacgcgcgg cgaagtgctg
     6601 ttctacaatg gcaagcctgc accaggcgcc aaataccatg cctgctgtgg gggcattact
     6661 gaaagcgcag gtgcgttgtg gcgattcgat gtgccgtaca tgataggcgt gggctgcggc
     6721 gtatcgaggc cggaggcggc tccggatagc ttgacggcta tccgcgatga ggaggcagta
     6781 cgtgctgcca tagccgcccc tgatccccgg gacttctgcc acggatccaa tgggtacaga
     6841 tggaaggtag agtacaccgc cggccagctg gcgacggtac ttgcgcccgt catcggccgc
     6901 cttgagccgc cgaatgcgag gatcattgga gtcacggtag tcgacaggac tccgcggggc
     6961 tctgctgttc gcgtaagggt gacgacgacc gagggcgaat acgaaatcgc gggagagtac
     7021 gcgatccgat cggcgctcgg cggcacggtc accatcaaga gcggtgtatt cgtcgtggat
     7081 accgaaggcg atccgccttc gttgttcgta ttctccgggg ctgggtacgg ccacggtgta
     7141 gggatgtgcc agtacggcgc tagaggaatg gctcgggcag gatacagcta ccgggcgata
     7201 ctcgggcatt actatccggg cacaacagta gaacccatac tccgctccgc cggcaattga
     7261 ggaacggtga tgatcgtgga tgacagaatc tacgagcgcg cgcaatctgg cgacaggagc
     7321 gcgatcaatg agctgttcga ggcatatgct gacactgcgg cgagaatggc aaggcagatg
     7381 gccggcgcat gccaggacct ggaggatatc gtccaggagg ctcttctgag ggcgtttcgc
     7441 tcgatcggca gcttcagatg ggatagctcc tttcagacat ggctctaccg cataaccgcc
     7501 aacgtatgcc gcgatttcgc ccgcaaatcc gccgtctgcc ggtgtgtgtc actggatgcc
     7561 ttgtgcagcg aggacgaagg cgccacagat gcggcatgcc aggctatgtc tactgtcgct
     7621 gctcagcctg aggcagaggc catccgccga gaacagcgtg aggcgattgt acgggctgtg
     7681 gctgcgcttc cccgtctgga tcgagctgtg ttcgtaatgc gcgagatcgg aagcttgtcg
     7741 tacaaggaca ttgccaactc gctcggatgc tcgatcgaga gcgtgcgctc acgcctggcc
     7801 cgtgcccgca ggcaggtgcg tacaaacatt caggcgttcc ttccggatgc ggctcggcca
     7861 tgacacgttt tcctccgcct ctcgtctaac ttaaagaggg gaggaatacg tgtgtcactg
     7921 gtcagggtag taaccgacag ctccaacggc atcccaccgg agtttgtgga gaagtacggg
     7981 atcgagatag tgcccattca ggtgcaattc ggcgccgatt cgtacaaaga gggtcgggac
     8041 ctatctttgg cggagttcta cagacggctg gatgaacctg tccttcccac cacttcgcag
     8101 ccctcgccag gcgatttcgt agagacgtac aagggactcg tgggcaagtg tgatgagatc
     8161 atctcgatcc atctcacatc aaagggcagc ggcacttgtc agtctgcccg gcttgcatca
     8221 cagatgatag aaggcgtgcg catcacgatt gtcgacaccc tcaccgcgag tatgggcaca
     8281 ggcctaattg tcatggccgc ggcagaggca gcctatcacg gatgcgggcg cgacgagata
     8341 atgcgtatca tccagagcgg catggacacg accagcgtgt tcgtggcgct tcctacgcta
     8401 aagtacctga ggcgaagcgg acgcgtcccg caagtgcagg cgtgggtggc cgggttgctt
     8461 tcaattcatc ccatccttgg cgtgcgtgat ggcgtggtcg aagcggttga gcgagtacgg
     8521 actttccgcg ggtgtgtgcg ccgtgttgtg
//
LOCUS       DAOTAC010000078         8489 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_7120_length_8489_cov_4.916054, whole genome shotgun sequence.
ACCESSION   DAOTAC010000078 DAOTAC010000000
VERSION     DAOTAC010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 8489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8489
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7120_length_8489_cov_4.916054"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /locus_tag="PLM74_04765"
     CDS             <1..721
                     /locus_tag="PLM74_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HON42431.1"
                     /translation="ATGAAGCEREIAKGVVASIRYSDGLSGFAQEPGECVNYVSCHDN
                     LTLWDKIARSNAFDSKDDRISMDLLAQAIVLTSQGIPFLEGGAELLRTKRGNHNSYRA
                     GDEINQFEWRRKHEFRQVFDYYRGLIALRRAHPAFRMITSAQVRSALKFIPGPPNTVL
                     FELDGSACGDEWGSIVVIYNANRHDVEIALPTQGEWSIVVAGRQAGTSPLDDAVYVVK
                     DDEDDDVRVRVPAISAMVLHS"
     gene            755..1699
                     /locus_tag="PLM74_04770"
     CDS             755..1699
                     /locus_tag="PLM74_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012448590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HON42432.1"
                     /translation="MPANLTPQYMEAEEAYKRAITPEERLAALEEMLRTIPKHKGTDK
                     MQADIKRRIARVKEAGEAARKKGKVDPYRVEREGAGQVFVVGPPNSGKSALVGRLTSA
                     KVVVAPYAFATTQPVPGMMKYEDILIQLVDTPPISADNCPPALMASVRSADAVIMVVD
                     VSSDSCLDDIQSLLDLLEERRVINQGKPHMVAMSKMDLPGAADNADILVEAYPGIGLV
                     RVSAESGEGLEELPRAVFDMLDIIRLYSKPPGRDVDRAAPFVLKRGGTVTDLAGAIHR
                     DLPRTMKSARVWGSARFDGQPVPREYVLSDGDIVEFRT"
     gene            1729..2235
                     /locus_tag="PLM74_04775"
     CDS             1729..2235
                     /locus_tag="PLM74_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42433.1"
                     /translation="MVRALIVYHSRMGVTEKLMRAIGDELEHSGHKVEYHKLEPARRP
                     GYLGAVWAALRKREAELKADLPKTLRTYELVLVAGPVHAGRTSAELNTFLNNMPDGIG
                     RRFGVFATMGSGSPEGQMKSIKTRIEKAGGLIVYEDAVRKGVVMNPDQLGVRARNIVE
                     QLLRPWVW"
     gene            2248..3498
                     /gene="pepT"
                     /locus_tag="PLM74_04780"
     CDS             2248..3498
                     /gene="pepT"
                     /locus_tag="PLM74_04780"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: protein motif:HMM:NF009920.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HON42434.1"
                     /translation="MSAEVLLSEGVAAKFMRYVQVDTTSAEDSAEVPSTPGQWELARM
                     LADELMQLGLIDVRVDEHCFVIGRLPSNMDQAAPAVGFVAHMDTVPGVPGRGVTPKAH
                     ASYQGGDISIGHGVVLSPKESPGLARALGCDIITSDGSTLLGADDKAGVAEIMQALVE
                     LIQNPDIRRPDVWVAFTPDEETGRGVERFPYAKFGAKFAYTLDGGVLGELSEETFNAL
                     NGVVTIEGVSAHTGTARGKMVNALHLAAQLISWIPSYCRPETTSGREGFIHPNDIQGN
                     VEKISIKFLIRDFDSEMLETRQATFMKMLELLEASYPGSRIRFTPSGGYRNMKERLDT
                     DPRVTAFAIRAMELAGLTPVVQPIRGGTDGARMTFEGVLTPNLFTGAGDVHSRREWAC
                     VQWMNDAVNVVINLVRLWAEQSNA"
     gene            complement(3647..3931)
                     /locus_tag="PLM74_04785"
     CDS             complement(3647..3931)
                     /locus_tag="PLM74_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009062130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HON42435.1"
                     /translation="MAYEDKTLVCKECGQEFVFTAGEQQFYVDHGFQNEPSRCPECRR
                     ARRASGGSRTMHEVVCSACGKTAMVPFVPSGDRPVYCSECFAAQKASDRR"
     gene            4129..4728
                     /locus_tag="PLM74_04790"
     CDS             4129..4728
                     /locus_tag="PLM74_04790"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HON42436.1"
                     /translation="MESISHEPKGTYVVSNIDELRALLDRKRVVILRMLAEEPLTVKQ
                     LADKMGLVPASVHYHVKVLERSGLVDLVGSKEKSGILEKYYRSIAREFQVDPSLGSAP
                     EAPGLALDALIRDMRSSISHLQQIGDTDPLINVQLVNVNLSRDAAARFADRIHELAEE
                     FKAAEEPGAQEAYTLALAVYPSASRPARHMGERGDGEEG"
     gene            4730..5743
                     /locus_tag="PLM74_04795"
     CDS             4730..5743
                     /locus_tag="PLM74_04795"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON42437.1"
                     /translation="MSHPAVELDRVARVFTAGAGRRRRSAVAVEELSMCVAPGEVMGL
                     LGPNGAGKSTVIAMISGLITPSSGRVLIGGIDAVRSRRQIAGRVGVVFDRARTLYPRM
                     TARENIEYFTSLRGGGRVKSVAGRAAHWIEFLGIGRWADVPVNNLSMGTQQKVSLAAA
                     LAAGPDVVLMDEPTLGLDVASSLDLQQLVRRMARDHGCALVIATHQMDVAESVCDRVC
                     IMSDGRALAIDTVSALLRVFGRRSYRIALRAPATDALQAAIVQAGARPSLASDVDAAR
                     GFVIDLDVDDDAGLPRALEAIRMAGTEVESVVRREPCLANVFLEITGTGKGQGADACC
                     SMA"
     gene            5721..6548
                     /locus_tag="PLM74_04800"
     CDS             5721..6548
                     /locus_tag="PLM74_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42438.1"
                     /translation="MHVVLWHELKRGLLRRARSPVEFITESLAAYIFLVVAVLAASGR
                     MPMPSGMAMPASGDPQGRAASEVMLAFACVFCCLGAVQGASLLATEEPGTAILEQLAM
                     SAFPLWVIALLRDIASFVSFIPSMALIMGAVSLTTGVPFPWPAAAALVPMAMMRLAML
                     GIGYALGAVALLVKRVGGLVNLVSMVVFALALVPVRQWGGVLGTLGIVFPYRWWYGLT
                     VDLTFRGNETAQLLAGGVARPGMALASIMTLVYFFAGLLAFNAAFRASLRRGSLSRA"
     gene            complement(6549..7616)
                     /locus_tag="PLM74_04805"
     CDS             complement(6549..7616)
                     /locus_tag="PLM74_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HON42439.1"
                     /translation="MLVKDSTISMSAQYARMRQESVSEKLEVRIGPERRSSGPTGPAV
                     GDQVSISARARAALAQADSLSAAESQALEGAITDETSVDPQIRMIKRIIELLTGVKID
                     TIELSKVLKGREGQPGAPGPQAQAQAQAVEPEWSVQYDREYAYHDLQYMSFEAEGTIV
                     TTGGEHIRFALSLSMHHEESVYNRTSLRMQNGKKVDPIIINLTGEAAQLADALFEFDL
                     NGDGKTEMIPELRRGSGFLVFDRNGDGIANDGTELFGPESGNGMDELAQLDSDGNGWL
                     DERDVAWTQLYVWTGGSRGRESLTPLAKAGVGAIYLGSVDTQFQMKNSSNRPVGEVAS
                     MGLYLREDKTVGSLQQIDLMV"
     gene            7759..>8489
                     /locus_tag="PLM74_04810"
     CDS             7759..>8489
                     /locus_tag="PLM74_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HON42440.1"
                     /translation="MITITLPDGKKHRAESGITLERLARVVPHGCRWPVMAARVDYDL
                     RELTYRLDNDARVEFVDLGSEDGMRIYRRTGILTLVMAAREVLQGCRVVIQHSLSNGL
                     YGEIRWRRPLTEAHVGAIEARMHEIARSNLPIERNSLTVSDAVELFRSDGQMDKVRLL
                     KYRRSGTVNIYQCGWLSDYLYGYMAPSTGYLRSFRLRHYLPGFILEFPRRTNPLELPE
                     YVEQGKLATVYFEAEKWGKVIGVTDV"
BASE COUNT         1651 a         2323 c         2757 g         1758 t
ORIGIN      
        1 cgccacaggc gctgcaggat gtgagcgcga gatcgcgaaa ggagtagtag cctcgattcg
       61 ctattccgac gggttgtccg gatttgctca agagcccggg gagtgcgtta actacgtgtc
      121 atgccatgac aacttgactt tgtgggacaa gatagcgcgg tccaacgctt tcgattcaaa
      181 ggatgaccgg attagcatgg atctcctggc acaggcgata gtcctcactt cgcaggggat
      241 accgttccta gagggcggag ccgagctgct ccgcaccaag cgggggaacc acaacagcta
      301 cagggccggc gacgagatca accagttcga atggcggcgc aagcatgaat tccgccaagt
      361 attcgactac tatcgcggtc ttatcgcgct gcgtcgggcg catccggcgt tccgaatgat
      421 cacttcagcg caggtgcgct cggctctgaa gttcattccg ggcccgccca acacggttct
      481 gttcgagttg gacggcagcg cgtgcggcga cgaatggggc tcgatagttg tcatatataa
      541 tgctaacagg cacgacgtgg aaatagctct tcctacacag ggagaatgga gcattgtggt
      601 tgcgggacgg caggccggga ctagccctct agatgatgcg gtatacgtgg taaaggacga
      661 cgaagacgac gacgttcgag ttcgtgtgcc tgcaatcagc gcaatggtgc tccacagctg
      721 accggacgca atagagcaaa ggagggaagc acgcatgccc gccaacctca ccccgcagta
      781 catggaggca gaggaggcgt acaagcgcgc aatcactccc gaagagaggc tcgcggcgct
      841 tgaagagatg cttcggacca tccccaagca taaggggacg gacaagatgc aggcggacat
      901 caagcgccgt attgctcggg tgaaggaagc cggcgaggcg gcccggaaga agggcaaggt
      961 cgatccctac cgtgtcgaaa gggagggggc agggcaggta tttgtggtag gcccgcccaa
     1021 ctcgggcaaa tccgcactgg tagggaggct tactagcgcg aaggtagtcg tggcgcctta
     1081 cgcttttgcg actactcagc ctgttccggg catgatgaaa tatgaagata ttctgataca
     1141 gctggtggac accccgccca tatcggctga caattgtccg cccgctctca tggcctcggt
     1201 tcgttctgca gacgcggtga ttatggtcgt ggatgtgtcg agcgactcgt gtcttgacga
     1261 catccagagc ctgctggacc tgttggaaga gcggagggtc atcaaccagg ggaagccgca
     1321 catggtggcc atgtcgaaga tggatttgcc gggcgcggcc gacaacgctg atatcctagt
     1381 ggaggcctat cccggcatcg gccttgtcag agtgtcggct gaatcaggag agggtctcga
     1441 ggagctgccc cgggccgtct tcgacatgct cgatatcatc cggctgtact caaaacctcc
     1501 tgggcgagat gtggaccgcg ccgcgccgtt cgtgctgaaa cgcgggggta ccgtgactga
     1561 ccttgccggt gccatccaca gggatcttcc gcgcaccatg aagagcgcca gggtatgggg
     1621 gtctgcccgc tttgacgggc aacctgttcc cagggagtac gtgctctctg atggagacat
     1681 cgttgagttt aggacatgat ttgtcgcgtt ttgggaggag gacgatgcgt ggtgagagct
     1741 ctgatcgttt accattcgcg aatgggcgta actgagaagt tgatgcgtgc catcggcgac
     1801 gagctggaac atagtggtca caaggtcgaa taccacaagc tggagccggc ccgacggcct
     1861 gggtatctgg gggcagtctg ggcggcgctc cggaagcgcg aggccgagct gaaggcggat
     1921 cttcccaaga cgcttcgcac ttacgagctg gttctcgtcg caggccctgt gcacgctggc
     1981 aggacctcgg ctgaactgaa tacctttctc aacaacatgc cggacggaat cggccgtcgg
     2041 ttcggcgtat ttgccacgat gggcagcgga agccctgagg gccagatgaa atcgatcaag
     2101 acgcgcatcg agaaggcggg cggcctgata gtgtacgagg acgctgttcg caagggagtc
     2161 gtcatgaacc cagatcagct aggtgtgcgg gcccggaata tcgtggaaca actcctgcgg
     2221 ccctgggtat ggtaggaggc gcatcgtatg agtgctgagg tattgctaag cgagggtgtt
     2281 gcggccaagt tcatgcggta tgttcaggtt gatacgacga gcgcggagga ctcggcggaa
     2341 gtgcccagca cgcctggaca gtgggaactc gcccgcatgc tggcagatga gctgatgcaa
     2401 cttgggctaa tcgacgtccg ggtggacgag cactgcttcg tgattgggcg tctgccttcg
     2461 aacatggacc aggccgcgcc ggcagtgggg ttcgtggccc acatggacac ggttccggga
     2521 gttcccggtc ggggcgtgac tccgaaagct cacgccagct accaaggcgg agatatctct
     2581 atcggtcatg gagtggtctt gagccccaaa gaatcgcctg gtcttgcgcg cgcgctcggc
     2641 tgcgacatca tcacgtcaga cggttctacg ttgctcggcg ccgacgacaa agcgggtgta
     2701 gccgagataa tgcaggccct ggtggaattg atccagaatc ccgacatccg caggccggac
     2761 gtgtgggttg cattcactcc tgatgaggag acaggtcgag gcgtagagcg gttcccgtac
     2821 gctaagttcg gcgcgaagtt cgcgtacact ctggacggag gggttctggg cgagctatcg
     2881 gaggagacgt tcaacgctct gaacggggtg gtcacaatag agggcgtgtc agcgcataca
     2941 ggcactgccc gcggcaagat ggtgaatgct cttcacctgg ctgcccagct catctcgtgg
     3001 attccttcat attgcaggcc agagacaact tcgggccgtg aaggtttcat acaccccaat
     3061 gacatccagg gcaatgtgga gaagatatcg atcaagttcc tgatacgcga tttcgatagc
     3121 gagatgctcg agacgcggca agccaccttc atgaagatgc ttgaactgct tgaggcgagt
     3181 taccccgggt cgagaatcag gttcacccct tcaggcgggt accgtaacat gaaagaacgg
     3241 ctggacacgg atccccgggt gactgccttc gcgattaggg caatggagtt ggctgggctc
     3301 actccggttg tgcagcccat caggggcggg actgacggcg cgaggatgac gttcgaaggc
     3361 gttttgactc ccaacctttt caccggggcc ggcgatgtgc acagccgccg ggaatgggcg
     3421 tgtgttcagt ggatgaacga cgcggtgaat gtcgtgatca acttggtgcg gctctgggca
     3481 gagcagagta acgcgtaggg ccgcatgggg cctacatgcg ccgtcagttc acatacgcaa
     3541 gcctcacatg cacacgcagg cggcgcccaa taggaaaacc ccgggtcatg cccggggtct
     3601 gtacttttcg ggtcaacatg tcttaggcgc catgagtctt cggatcctac ctgcgatcgg
     3661 atgccttctg cgccgcaaag cactcgctgc agtagacagg cctgtcgccg ctgggaacaa
     3721 atgggaccat ggccgtcttt ccgcacgccg agcagactac ctcgtgcatc gtgcggctcc
     3781 caccgcttgc tctgcgggca cgcctgcatt cagggcacct ggagggctca ttctggaagc
     3841 cgtggtcaac gtaaaactgc tgctcgccag cggtgaacac aaactcctgg ccgcattctt
     3901 tgcagaccag cgtcttgtcc tcgtacgcca acctgatcgc gctcctttgc ttgggtttga
     3961 gctcttggtc aagtcggcat actcttcgga tgcgcgcgct tgaacaacag ccgatatcag
     4021 attaccactg aaaggcttaa aatgcaagat cactgggaat cagccagttg acacccattc
     4081 gaattaccgt tatcattaaa gaaacgttta tcgagagggg aaaccgtaat ggaatcgata
     4141 tcgcacgaac ccaaagggac ttatgtcgtg agcaacatcg atgagctacg ggctttgctc
     4201 gataggaaga gagttgtcat ccttcgtatg ctggctgagg agccgctcac cgtgaagcaa
     4261 cttgccgaca agatgggcct cgtgcctgcg agtgtgcact accacgtcaa ggtgctggag
     4321 cgctccgggc tagtcgattt ggtcggcagc aaagagaagt ccggaatatt ggagaagtac
     4381 tatcgctcca tagcgaggga gttccaggta gatccatcgc taggcagtgc gccggaggct
     4441 cccggcctgg cgctggatgc cttgatccgc gacatgcggt catccatcag tcatctacag
     4501 cagatcggcg acacggatcc actgatcaac gtgcagttgg tgaacgtgaa cctgagccgc
     4561 gacgcggcag cgagattcgc cgaccgtatt catgagcttg ccgaggagtt caaggcggcg
     4621 gaagaaccag gggcacagga ggcatacacg ctggcccttg cggtgtaccc atcagcgtcc
     4681 cgaccggccc gacatatggg cgaaaggggc gatggggagg aagggtgaga tgagccatcc
     4741 cgcggttgaa cttgatcggg tcgcccgcgt gttcaccgct ggagcagggc gacgtcggcg
     4801 atcggcagtg gctgtcgagg agttgtcgat gtgtgtcgct cccggtgagg tgatggggct
     4861 tttgggtcca aacggggcgg gcaagagtac ggtcattgcg atgattagcg gtctcatcac
     4921 tcccagttcc ggtcgcgtgc ttatcggggg tatcgacgcc gtgcggtcac gacggcagat
     4981 cgcgggtcgg gtgggcgttg tgttcgacag ggcgcgcaca ctgtacccaa ggatgaccgc
     5041 gcgcgagaac atagagtact ttacctccct tcgaggcggc ggcagggtga agagtgttgc
     5101 tggccgagct gcgcattgga tcgagttcct aggcataggc cggtgggcgg atgtgcctgt
     5161 gaacaatctc tccatgggca cgcagcagaa ggtgtcgctg gcggcggctc ttgctgctgg
     5221 ccccgacgta gtcctaatgg acgagccaac ccttggcctc gatgtagcgt catctctcga
     5281 cttgcagcag ttggtcaggc gcatggcgcg cgaccacggc tgcgctctgg tgattgccac
     5341 gcaccagatg gatgtggccg aaagtgtgtg cgatagagta tgcataatga gcgatggcag
     5401 agcgctggcc atcgacactg tgagtgccct gctcagggtg tttggccgca ggtcttacag
     5461 aatcgcactg cgcgcccccg ctactgatgc cctgcaggcg gcgatcgtgc aggcgggagc
     5521 gcgaccgagc ctggcctcgg acgtcgatgc cgcgagaggt ttcgttatcg atcttgacgt
     5581 tgatgatgat gccgggctgc cgagggccct agaggcgatc aggatggcag gcactgaggt
     5641 agaatcggta gtcaggcggg aaccgtgcct ggccaacgtg tttctcgaga ttacaggaac
     5701 cggaaagggg cagggggcgg atgcatgttg ttctatggca tgagttgaag cgggggttgc
     5761 tgcgccgagc gcgcagcccg gtcgagttca tcaccgagtc gctggcggca tacatattcc
     5821 tggtggttgc ggtgttggct gcgtccggcc gtatgcccat gccgtcgggt atggcgatgc
     5881 cggcatccgg cgaccctcag ggcagggctg cgagcgaggt gatgctcgcc tttgcatgtg
     5941 tattttgttg cttaggagcg gttcaggggg cctcgcttct cgcgacagag gaacccggca
     6001 cggccatcct ggagcagctg gcgatgtcgg cctttccatt gtgggtgatt gcgctgctgc
     6061 gcgatattgc gtcctttgtc agcttcattc caagcatggc gttgatcatg ggggccgtat
     6121 cgctcaccac aggggtcccg tttccctggc ccgctgccgc tgccctggtg cccatggcca
     6181 tgatgcgcct ggccatgctg ggcatcgggt atgctcttgg agcggttgcc cttctggtca
     6241 agcgagtcgg aggcctggtg aatctggtgt cgatggtggt ttttgctcta gcgctcgttc
     6301 ccgtccgaca gtggggaggc gtgctcggga cgttgggcat tgtgtttcca tatcgttggt
     6361 ggtatgggct cacggtagac cttacattca gaggaaacga aacggcccag cttctcgccg
     6421 gaggagtcgc taggcctggc atggcgctgg cttctatcat gactcttgtc tatttcttcg
     6481 cagggctcct ggcgttcaac gctgcgttcc gcgcctcgct gcgtaggggt tcgctctcca
     6541 gagcctgatc acaccatcag atcgatctgc tgaaggctcc ccacggtctt gtcttcacgg
     6601 aggtataggc ccatgctggc cacctcgccc acgggacggt tggagctgtt tttcatctgg
     6661 aactgggtat cgacggatcc gaggtagatg gcgcccacgc cggctttggc cagtggagtc
     6721 agggactcgc gaccgcgaga tccgccggtc cacacataca gctgggtcca ggccacatcg
     6781 cgttcgtcga gccacccgtt gccatctgaa tcgagctggg ccagttcgtc catgccattg
     6841 ccgctttcgg ggccgaacag ctcggtcccg tcgttggcta tgccgtctcc gtttctgtcg
     6901 aagaccagaa agccgctgcc gcgtcgcagt tcggggatca tctctgtctt tccgtctccg
     6961 ttcaggtcga actcgaagag cgcgtcggca agctgtgcgg cctcgcctgt gagattgatg
     7021 atgatcgggt cgaccttctt gccgttctgc atccgtaagg aagtccggtt gtacacggat
     7081 tcctcgtgat gcatcgatag gctcagggcg aacctgatat gctcgccgcc ggtagtgact
     7141 atggtgccct ccgcttcgaa ggacatgtac tggaggtcat gataggcgta ttcgcggtcg
     7201 tactgcacgc tccactcagg ctccacggcc tgggcctggg cctgagcttg tggcccgggc
     7261 gcgcccggct ggccttcgcg acccttaagc actttggata gctcgatcgt gtcgattttg
     7321 acgcctgtca gcagctcgat tatccgcttg atcattcgga tctgcggatc gacggaggtc
     7381 tcatccgtga tagctccctc cagcgcctgc gattcggcgg cggaaagcga gtcggcttgg
     7441 gccagggcag cgcgagcgcg ggcggatatg ctgacctggt cacccactgc cggaccggtc
     7501 ggacccgacg agcggcgctc gggtccgatc cgcacctcaa gcttttcgga tacgctctcc
     7561 tgtcgcatgc gcgcatactg ggcggacatc gatattgtcg agtccttcac tagcatccgt
     7621 gcattccccc ttgatgcgcc gatatgggct ctgctgatgt tatcggcaat tggcgccgcg
     7681 actttacccc cagcgcgcat cgatgtatca ttgtcccaga gcattgcttc gcgcaactat
     7741 ggatcgggag aggacgacat gatcacgatt acccttcctg acggcaagaa gcaccgggcg
     7801 gagtcgggaa ttacgctcga acgcttggcg cgcgtagtgc cacacggatg tcgatggccg
     7861 gtgatggctg ccagggtcga ttatgacctg agagagctga cctacaggct ggacaatgat
     7921 gcccgagtcg agttcgtaga tctgggctcc gaggacggca tgaggatcta caggcgcaca
     7981 ggcatcctca ctctggtaat ggcggccagg gaggtgttgc agggctgtcg cgtagtgatt
     8041 cagcattcac tatcgaacgg gctctacggg gagataaggt ggaggcgacc gctcacagag
     8101 gcgcacgtcg gcgccattga ggcgcggatg cacgagatcg cccggagcaa tctgcctatc
     8161 gagcgcaata gtctcacagt gtcggatgca gtcgagctgt ttcgcagcga tggccagatg
     8221 gacaaggtgc gtctgctgaa ataccgtaga tccggtaccg tgaacatata ccagtgcggg
     8281 tggctcagcg actacttgta cgggtacatg gcgcccagta ccggctatct gaggtccttt
     8341 cgacttcggc actacctgcc cggtttcatc ctggagttcc ccaggcgcac aaaccctttg
     8401 gagttgcccg aatacgtgga acagggcaag cttgccaccg tgtacttcga ggcggagaaa
     8461 tggggcaagg taatcggcgt gacggatgt
//
LOCUS       DAOTAC010000079         8475 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_7142_length_8475_cov_5.126247, whole genome shotgun sequence.
ACCESSION   DAOTAC010000079 DAOTAC010000000
VERSION     DAOTAC010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 8475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8475
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7142_length_8475_cov_5.126247"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..848)
                     /gene="mgtE"
                     /locus_tag="PLM74_04815"
     CDS             complement(<1..848)
                     /gene="mgtE"
                     /locus_tag="PLM74_04815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HON42441.1"
                     /translation="MDDRYTVLVEKVKQLLSDGSTEALTGLVDSLHPADVVDILELLD
                     DSARSAFFHAIPADKAADIVQELDPDDQTEIIRMMGAARSSEILREMDSDDAADLIGE
                     LPESKAETLLELMNSEGDEVRELLQYDEDSSGGIMATEYVTVNMEWTVEKTLAQLRHA
                     APEAETVYYIYVVDADERLVGVLSLRDLVIADLKRKISSIMSRNVLSVPVEADQEEAA
                     QLFKKYRYLALPVVDRDNRLAGIITADDILDVVEEEATEDIQKIAGTMPLDRPYFATS
                     VKELWR"
     gene            1249..2202
                     /locus_tag="PLM74_04820"
     CDS             1249..2202
                     /locus_tag="PLM74_04820"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HON42442.1"
                     /translation="MSGLREAALVAVRDLMGATPGETLLVVMDDGTRSVGYALYQAAA
                     ELGCEAMAIEMIERRSHGEEPPAPVAQVMKHVDIVLAPTSKSISHTSARAEACAAGAR
                     CATLPGITEDCMARALGADYEAVGVRSRKYADVLTRGATVRITNTEGTDITMSLAGRT
                     GNADTGVYHNPGDFGNLPAGEAYIAPLEGTANGVFVVDGAMAGAGHLGERIKINVRDG
                     YATDIEGGAAAAALQQMIDPLGRLARNIAELGIGTNEKARITGTVLEDEKVMGTIHIA
                     LGDNSHMGGTVAVPSHLDGIVLAPTVVVDGVTIMESGKLIL"
     gene            2281..2496
                     /locus_tag="PLM74_04825"
     CDS             2281..2496
                     /locus_tag="PLM74_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42443.1"
                     /translation="MGDRILESPARSRVRKRVDGRRRPADFADSDIVDAILVEVEWED
                     EGNSDAPLDGWWSDRWGSSARHSAGDE"
     gene            2587..5715
                     /locus_tag="PLM74_04830"
     CDS             2587..5715
                     /locus_tag="PLM74_04830"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HON42444.1"
                     /translation="MDGAARTDKLGQRLCELGMGACAVTDHGSLFGAVDFYRTCRECG
                     VKPIIGCEVYVANRTRHDRTPRVDDDPYHLVLLAENQQGYKNLLKLSSIGYTEGFYYK
                     PRVDKEVLSAHSSGLIALSACLSGEVSAYIARGQAETAERAAAEYRDIFGPGSFFLEV
                     QANGMREQERVNRGIAAIGAKMGIPLVATNDVHYLRREDARAHDVLLCIQTGASVDDP
                     GRLRFPTDQFYLKSPDEMYRQFPEMPQACAETVRIAERCSVDLDFNTLHIPEFAPPEG
                     ETLDSYLRRVCMEGAARRFGLMSDTVRARLDYELDVIARMGYSGYFLIVWDFIRFARS
                     RGIYVGPGRGSGPGSLVAYCLGITNIDPLRYNLLFERFLNPERVTMPDFDIDFCWERR
                     GEVIDYVTRKYGADRVAQIITFGTMAARAAIRDVGRALKVSYGDVDRIAKLVPAELNM
                     TIDRALEMSPELATAAAENERIRMMVDVARAVEGMPRHPSVHAAGVVISKEPLTEHVP
                     LYKSGDGVVTTQYPMEDLERLGIVKMDFLGLRTLTVIGNTVEIVRHTRGEELDIGDIP
                     LDDPEVYEMLRRADSAGVFQLESSLFQNLLREVKPTRFEDIIALIALGRPGPMVMTGD
                     FVRGKHDQASVTYLHPALKRILEPTYGVMLYQEQVMQVASELAGFSLGEADVLRRAMG
                     KKKPEAILGLKGRFLDGARERGVSDEVAAEVFSLIERFAGYGFNKAHSAAYALISYQT
                     AYLRCHYFPEFMAATLTSVMSAPERVAMYIDACRCRGVEVLGPDVNDSLKGFTVRGNT
                     IRFGIAAVRNVGEAAADAIIAARKAGGKFMSLSDFCDRVDMAAVSRKALDSLIRCGAF
                     RAFGSRRALLAVMDQICDASAARQRQQQSGQASFFDLFERPSQFGQAEVTLPSVPEFR
                     QDQILAMEKELLGLYISGHPLAFLSDTISKLSTVAVRDLPDVPDGASVTLAGVVAGRK
                     QISTKTGQPMAFVQIEDMTGQAEVVVFPRVYEQYAQLLAPDAPVTVRGRVDVKEDGVK
                     VLADTVSALPSS"
     gene            5852..6043
                     /locus_tag="PLM74_04835"
     CDS             5852..6043
                     /locus_tag="PLM74_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HON42445.1"
                     /translation="MWAVVHIAPNRAEAELTKHMLEAEGILVMLRPMGIPHMGDSASV
                     EVLVPETEAEEARNILDEY"
     gene            6117..7118
                     /gene="pfkA"
                     /locus_tag="PLM74_04840"
     CDS             6117..7118
                     /gene="pfkA"
                     /locus_tag="PLM74_04840"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HON42446.1"
                     /translation="MRRIAVVTSGGDAPGMNACIRAVVRKALYHGLEVVGVRRGFHGL
                     VAGDFAEMKSRTVGDILQRGGTILKSARSEEFRTEEGRAKALLQLRTYEIDGVIGIGG
                     DGTHAGLNLLSKMGMPTVGIPATIDNDIGSTDSAIGFDTACNTVIDAINKIRDTATSH
                     ERTYVVEVMGRNSGHIALAAGLAGGAESILIPEVEFDIQQVCERITAGARQGKSHSIV
                     VVAEGAEGAVSPGKGGLPGGCAYRIGQQIAELTGFETRIIVLGHIQRGGAPSVRDRIL
                     ATTLGAKAVDVLLSGLGSGVVGVLGNEIRVMSFAEALSMKRMINMDYYELANILSNL"
     gene            7234..7767
                     /locus_tag="PLM74_04845"
     CDS             7234..7767
                     /locus_tag="PLM74_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation T C-terminal
                     domain-containing protein"
                     /protein_id="HON42447.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRRTLRIREGDPLEIFTDRDGEV
                     ILKKYSPMTDLSFFAQEYADSLYDATGCIAAIADDHEVVAVAGAPKKQFVEKRISEQA
                     EEIMKSRETLVSNVPNENPVVDNAQFESQVMAPIHAGDEPQGLVVLMSKEPRQMGSTE
                     TKLVETAAAFLGKQLLQ"
     gene            8390..8475
                     /locus_tag="PLM74_04850"
     tRNA            8390..8475
                     /locus_tag="PLM74_04850"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8423..8425,aa:Leu,seq:caa)
BASE COUNT         1733 a         2292 c         2757 g         1693 t
ORIGIN      
        1 ctgcgccaga gctccttgac tgaggtcgcg aagtacggtc ggtcgagagg catggtgcct
       61 gcgatcttct ggatgtcctc cgtggcctct tcttcgacta catccagtat gtcgtcggcg
      121 gtgatgattc ctgccaggcg gttgtcgcgg tcgacaacgg gcagagccag gtagcggtat
      181 ttcttgaata gctgggccgc ttcttcctgg tcggcctcaa ccggcaccga cagcacattc
      241 cgactcataa tggaggatat cttgcgcttg agatcggcga tgaccagatc acgcagggag
      301 agtaccccta cgaggcgctc atctgcgtcg acgacgtaga tgtagtacac cgtttccgcc
      361 tctggggcgg catgtcgcag ctgggcaagg gttttttcga cggtccactc catgttgact
      421 gtgacgtatt cggtggccat gataccgccg gaggagtcct cgtcgtactg cagaagctcg
      481 cggacttcgt ctccttcgga gttcatcagt tcgagcaggg tctcggcttt cgactccgga
      541 agctcgccga tgaggtcggc ggcatcgtcc gagtccattt ccctcaggat ctcggatgag
      601 cgggcagcgc ccatcatccg gattatctct gtctggtcat ccggatccag ctcctggact
      661 atgtcggcag ccttatcagc tggtatggcg tggaagaaag cactgcgggc tgagtcgtcg
      721 agtagttcca agatgtcgac tacgtccgcg ggatgtaggg agtctacgag gccggtcagc
      781 gcttcagtag agccgtcgga aagcagctgc ttgacttttt ctacaaggac agtgtacctg
      841 tcatccacaa caacaccccc ttctaggggc gcacgcaccg acctgccggc acggtgaaca
      901 tcgccccaga ctggttttga gcgggtctac tcgacttcga cgcagggctt agcagtatga
      961 accgcttcgt actgcctggg tctccgtcca ccaattttcc ccccttgttg atcaatatga
     1021 ggtgagcgcg gagagcctct cacgggctca ccctggtaat tatactatct cctctccagg
     1081 atgtcactcc tcgagcatcc tggtgcatcc tggacgcaag ttccgggttc cgctcggtgg
     1141 ccatcgggta accttcaggc gaccatcggg cgacgtatgg gatgactcgc ccgaatgtgg
     1201 tagaattagc ttatgagctg ttacgtgggt atgaggggga gttgcgtgat gtccggtctt
     1261 cgggaggcgg cgcttgtggc cgttcgcgac ttgatggggg cgacccctgg ggagactctc
     1321 ctcgtagtaa tggatgacgg cacccgcagt gtgggttacg cgttgtacca ggctgcggcg
     1381 gagcttgggt gtgaggccat ggccatcgag atgatcgagc gcagaagcca tggtgaggaa
     1441 ccccctgcgc cggtagcgca agtcatgaag catgtggata ttgtgctcgc acccaccagc
     1501 aagtctatca gtcatactag cgccagggcc gaagcgtgcg ctgcgggggc gcggtgtgct
     1561 acactgcccg gcattactga ggactgcatg gcgagggctc tgggcgccga ttacgaggca
     1621 gtgggtgtgc ggtcgaggaa atatgccgat gtgctcacac ggggcgccac tgtgaggatc
     1681 accaacacag aaggcaccga cattactatg tcgcttgccg gcagaacggg caatgccgac
     1741 acaggtgtat accacaatcc gggcgatttc gggaacctgc ctgcaggcga ggcttacatt
     1801 gctccgcttg agggcacggc taacggcgtg tttgtggttg acggggccat ggcgggcgcc
     1861 ggtcatctcg gcgagcgtat caagataaac gtgcgcgacg gctacgccac agatatcgaa
     1921 ggcggcgctg ccgctgccgc acttcagcag atgatcgacc ctcttggccg cctggccagg
     1981 aacatagctg aactcggtat cggcactaat gagaaggcca ggatcaccgg cacggtcctt
     2041 gaggatgaga aggttatggg caccatacac atagcgcttg gcgacaatag ccatatggga
     2101 ggcacggtag ctgtgcccag ccatctggac ggaattgtgc tcgctcccac cgttgttgtg
     2161 gatggtgtga cgataatgga atcagggaag cttatcctgt agcgttcgct ctcgagccag
     2221 cgacggttgg ggtctgcgca tttccatccg gaacacgttt accaggagga ggagatacgg
     2281 atgggagacc gcatcttgga gagcccggcg cggtcgcgcg tccgcaagcg tgtggatggc
     2341 cgcaggagac cggcggattt cgcggacagc gacattgtcg acgcgatcct tgtcgaggtc
     2401 gagtgggagg acgaagggaa ttccgacgct cctttagacg gatggtggtc ggaccgatgg
     2461 ggttcgtccg cgcgccattc tgcgggcgac gagtaaaacg cagggtggcc tggcggcagc
     2521 cgggggggtg atcatgtgag ggagtttgtc catctacatc tgcatactca gtactccctc
     2581 ctgtgagtgg acggggctgc gaggacggac aagctggggc aacgactgtg cgagttgggc
     2641 atgggcgcct gtgctgtcac cgaccacgga tcgctgttcg gagcggtaga cttctaccgt
     2701 acctgccgcg agtgcggagt caagccgatc attggctgtg aggtctacgt ggccaaccgc
     2761 accaggcacg accgcacccc gcgggtagac gacgacccct atcacctggt gcttctggcc
     2821 gaaaaccagc agggctacaa gaaccttttg aaattgtcaa gcatcggcta caccgaaggc
     2881 ttctactata agccgagggt cgacaaggag gtcctgtcgg cgcactccag cgggctcata
     2941 gcgctctcgg catgcctctc aggcgaggtg tctgcctaca tcgcgcgtgg gcaggctgag
     3001 acggctgagc gggcagcagc ggagtatcgc gatatcttcg gcccgggctc attcttcctg
     3061 gaagtgcagg ccaacggtat gcgagagcag gagcgcgtga atcgcgggat agctgcgata
     3121 ggcgcaaaga tgggaatacc actcgtggct acgaacgatg tgcattacct ccgtcgcgag
     3181 gatgccaggg cccatgatgt gctcctttgc atacagaccg gcgcttcggt ggacgatccg
     3241 gggcgcttgc gttttccgac cgatcagttc tacttgaagt cgccggacga gatgtatcgg
     3301 cagtttcccg agatgccgca agcgtgcgcg gagacggtgc ggatcgcgga acgttgcagc
     3361 gtcgatctgg atttcaacac tctccatatc cccgagttcg cgccgccgga gggtgaaacc
     3421 ctcgatagct acctgaggcg ggtgtgcatg gagggcgccg cgcggcgttt cggcctcatg
     3481 tcggacacgg tgcgcgcccg gctcgactat gagctggatg tcatagcgcg catgggttac
     3541 tccggctact tcctgatcgt ctgggatttc atcaggtttg ccaggagccg gggcatatac
     3601 gtaggccccg gccgaggatc ggggccggga agcctggttg cgtactgtct gggcattacc
     3661 aacatcgatc cactccggta caacctgcta ttcgagaggt tcctcaaccc ggaacgcgtg
     3721 accatgcccg acttcgacat cgacttctgc tgggagcggc ggggcgaagt tatcgactac
     3781 gtcacacgca aatacggggc ggatagggtt gcacagatca tcaccttcgg caccatggct
     3841 gcacgcgccg ctatccggga cgtcggccgg gcacttaagg tcagttacgg agatgtcgac
     3901 cgcatagcca agctggtgcc ggccgaactc aacatgacca ttgaccgcgc cctggagatg
     3961 tcgcctgaac tggccacggc tgcggctgag aacgagcgaa ttcgcatgat ggttgatgta
     4021 gcccgcgcag tggaagggat gcctcgtcac ccgtcggttc atgccgcggg agttgtaata
     4081 tcgaaggaac ctttgaccga gcacgtacct ctatataagt cgggggacgg agtggtaacc
     4141 actcagtacc ccatggaaga cctcgaacga ttgggcatcg tgaagatgga cttccttggc
     4201 ctgcgcacac tgacagtgat cggcaacacc gtcgagatcg tgcgccacac gcgaggcgag
     4261 gaactggaca tcggcgatat acccctggac gatccggaag tctacgagat gctgcggcgt
     4321 gccgattcgg ccggcgtgtt ccagcttgaa agcagtctat ttcagaacct gctccgcgag
     4381 gtgaaaccca cgcggttcga agatatcatt gctctgatcg ctttgggcag gccgggccca
     4441 atggtgatga caggcgattt cgtccggggc aagcacgatc aggccagcgt aacgtatctc
     4501 catccggcgc tcaagaggat cttggagcct acgtacgggg tgatgctcta ccaggagcag
     4561 gttatgcagg tcgctagcga gcttgcaggc ttcagcctgg gagaggccga tgtgctgcgg
     4621 cgcgccatgg gcaagaagaa gcccgaagcc attctggggc tcaagggcag atttctggat
     4681 ggggcccgcg agcgtggcgt ttcggacgag gttgccgcag aggttttctc tctcatagag
     4741 aggtttgcgg gttacgggtt caacaaggct catagcgcgg cgtacgctct gatatcgtat
     4801 cagaccgcct atctgcgatg ccactacttt ccagagttta tggctgcgac acttacgagc
     4861 gtgatgagcg ctcctgagcg tgtcgcaatg tacatagatg catgtcggtg taggggcgtc
     4921 gaagtactgg ggccggacgt gaacgacagc cttaaaggct tcacggtacg tggcaacacg
     4981 atccgtttcg gcatagccgc cgtgaggaac gtgggcgaag ctgcggccga cgccataatt
     5041 gctgcgcgca aagcgggagg caagtttatg tcgctttccg atttctgtga ccgtgtggac
     5101 atggctgccg tcagccgcaa agcgctcgat agtctgatca ggtgcggcgc cttccgcgcg
     5161 ttcggaagca ggcgggcgct cttggcagtg atggaccaga tctgcgacgc gagcgctgcc
     5221 aggcagcgtc agcagcagtc ggggcaggcg tccttcttcg atcttttcga aaggccgtcg
     5281 cagtttggac aggctgaggt gaccctcccg agcgtgccgg agttcaggca agatcagata
     5341 ctggccatgg agaaggagtt gctcggcctg tatatatccg ggcatccgct ggcgtttctc
     5401 tcggacacga taagtaagct gtccaccgtg gctgtccggg acctccctga tgttccggat
     5461 ggtgcgagcg tgactttagc gggagtggtc gccggccgaa aacagatatc caccaagact
     5521 gggcagccga tggcttttgt ccagatagag gacatgaccg ggcaggcaga ggtggttgtg
     5581 ttcccacggg tgtacgagca gtacgctcag cttcttgccc ctgatgctcc ggtgacggtg
     5641 aggggaagag tggatgtcaa ggaggacggg gtgaaagtgc tggcagacac agtttcagcc
     5701 ttgccgtcat cgtaggtcga gaacgtggaa ggagaatcgg tgctagaatg gaacatcgga
     5761 cataataagc gggcaaggcc ggatagtcga ggtgcaggac atccgaaatg ccgagcgtac
     5821 gtaaccgcgc ttgagatggg gggcggctgg cgtgtgggcg gttgtacata tcgcgccaaa
     5881 tagagcagaa gcagagctta caaagcacat gctggaggcg gaaggaatcc tagtgatgct
     5941 gaggcccatg ggaattcctc acatgggcga ctcggcgagc gttgaggtcc ttgttccgga
     6001 aaccgaagcc gaggaggccc ggaacatact ggacgaatac tgatcatgat aacttaatgt
     6061 cctaatggcc taatggccgg ggcatgatct gtcctcgtgt gatgggggga tccgatatga
     6121 gacgaatcgc ggtagttacc agcggcggcg acgcgcccgg gatgaacgcc tgcatccgag
     6181 ccgtggtaag gaaggctctt taccacgggc tggaggttgt gggcgtcaga cggggttttc
     6241 atggtctggt cgctggggat tttgctgaga tgaaatcacg cactgttggc gacattcttc
     6301 agcgtggtgg cactattctg aaatcggcgc gctcagagga gttcaggacc gaagagggac
     6361 gcgccaaggc cctgctccag ctgaggacat acgagatcga tggcgtcatc ggcatcggag
     6421 gcgatgggac acatgccggc ctcaatctgc tttccaaaat gggtatgccg actgtgggca
     6481 tacctgcgac tatcgataac gacatcggtt ccaccgattc cgccatcggg ttcgatactg
     6541 cctgcaacac ggtgatcgac gcgataaaca agatacgaga taccgccaca tcacacgagc
     6601 gaacctacgt tgtggaggtg atggggagaa actcgggcca tatcgcgttg gctgcagggt
     6661 tagccggcgg cgccgagagc atactcattc cagaggtcga gttcgatatt cagcaggttt
     6721 gcgagaggat aaccgcaggt gcccgccagg gaaagagcca tagcatagtc gtggtggcag
     6781 agggagcaga aggggccgtc tctcctggca agggcgggct gccgggcgga tgcgcctatc
     6841 gaataggcca gcagattgcg gagctcaccg gtttcgagac cagaatcatc gtgttgggcc
     6901 acatccagcg gggaggcgcg ccgtcggttc gcgaccggat actagcaacg acacttggcg
     6961 ccaaagccgt cgacgtactg ctgtcggggt tgggtagcgg tgtagtaggt gtactcggaa
     7021 atgagattcg cgtcatgtcc tttgccgagg cgctctcgat gaagcgcatg atcaacatgg
     7081 attactacga gcttgcgaac atactttcaa atctgtaggt tgccgtccgc cacacatgcg
     7141 gccatcgcgg tgtaaggccc atgggtatat ggccgaaaca tcgaaggcaa aatagcacca
     7201 aagacggcgg aaccacgttg gggagggcgt aaattgaaag cgacggggat tgtacgacgt
     7261 attgatgatc taggacgagt cgtgataccc aaggaaatca ggcggactct cagaatccgt
     7321 gagggcgatc ctctggagat attcaccgat cgagatggag aggtcatcct caagaagtac
     7381 tcgcccatga cggatctgag cttcttcgcg caggaatacg cggactcact gtacgacgcc
     7441 acgggatgca tcgcggcgat cgcagacgac cacgaagtcg tagcggtggc cggcgctccc
     7501 aaaaagcagt ttgtggagaa aaggatctcg gagcaggctg aggaaatcat gaagtcccgc
     7561 gaaaccctgg tgtcgaacgt accaaacgaa aacccggtag tcgacaacgc tcagttcgag
     7621 agccaggtca tggcgccgat tcatgcaggc gacgaacctc agggcctggt agttctgatg
     7681 tccaaggaac cacggcagat gggatcgacc gaaaccaagc tcgtggagac agccgctgcg
     7741 ttcctgggca agcagctact gcagtagatc cgcctcgcac gtccgcggac ccgatatcgt
     7801 cgccgaacgg cccggcagtt tttgcgcaac cgagcgcgcg aaactggcct gggttgctgc
     7861 ttggcatcgc ctacaccccg ggcggggcgc gccgaatcag ccccgtgatg ggtgacgtat
     7921 ccactgactc tgacaaacct gctggaatac gttcaagccc tcgtgaaggc ttacgcgtgt
     7981 tccagcaggt tctgtttgcc gcaagaacga tcaccgtgca cagcgtctcg gagatctgcg
     8041 gcgtttccgc ctcccgccga gaccggcaac cttctcccgg gccgcggggt cgagggcgac
     8101 ggtttcagcc catgccctct agctggggtt ctcgtggctg cgggttctgc tgggcggagc
     8161 gtcggacgaa ggccgctgcg gacctgtgcc ctgtgggaca ggattttcgc ggggcctggg
     8221 taaaccgaca caggatggcc caaaaaggtg tcccgtaggg cacaggatgc ggccggcaac
     8281 cttctcccgg gccgcggggt cgagggcgac cgtttcagcc cacgtgtgga gacgcacgaa
     8341 tctgctgtgt agttgcgtgc gagccaggcc cgtggtagaa taatgctgcg ccgaagtggc
     8401 ggaacggcag acgcagatga ctcaaaatca tccgatcgca aggtcgtgag ggttcgaatc
     8461 cctccttcgg cacca
//
LOCUS       DAOTAC010000080         8456 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_7160_length_8456_cov_4.631472, whole genome shotgun sequence.
ACCESSION   DAOTAC010000080 DAOTAC010000000
VERSION     DAOTAC010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 8456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8456
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7160_length_8456_cov_4.631472"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..1430)
                     /gene="pyk"
                     /locus_tag="PLM74_04855"
     CDS             complement(12..1430)
                     /gene="pyk"
                     /locus_tag="PLM74_04855"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HON42448.1"
                     /translation="MRKTKIVCTIGPASHGLDSLRKLIEAGMDVARLNFSHGTHAEHA
                     ERIRMIRELEAEYHRYIPIMLDTKGPELRIGRFASGPITLEPGASFTITTEPVMGGPN
                     VVSVNYPGLPLIVAPNDRLLLDDGLLELEVTAVQGQAVECKVVVGGQLSDRKRISIPG
                     RRLNLPTLSADDKDDLLFGIENGVDLIAASFMRSAADLQQVKSFLAEHGSSIPVIAKI
                     ESREGIESLDSILRAADGLMVARGDLGVEMPAEDVPIIQKQMIQKCRRAGKPVITATQ
                     MLDSMIRNPSPTRAEVSDVANAVFDGTDAVMLSGETASGMYPVESVRTMARIAERAEM
                     ALQYTDAATPRMGSASMPVTDAIGHATVQIAAELRAAAIITSTKSGWTARMISKHRPR
                     TMIIAVATDMHTTRTLNLLWGVYPILGEDITTTDEMILIAVNKAVEAGYARPGDTVVI
                     TAGVPVGVSGTTNLIKVHEIRP"
     gene            complement(1445..1990)
                     /locus_tag="PLM74_04860"
     CDS             complement(1445..1990)
                     /locus_tag="PLM74_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42449.1"
                     /translation="MRRNIEGDSKDGRWAPVSDIFDKIKKTIGVGVDTVGAKSKELWD
                     TTVIRARISDLKAKRRDLLEDLGRLTFEMLSGGSLAEPVLRAKADAIHEIDRQIAAAE
                     EEIQRIREASREQQQADAEDVPAQPAEVDVPESTDESPACDGEPSVLEEPKPLRTCEC
                     GTLVLEGARFCVACGRPVSED"
     gene            2210..3607
                     /locus_tag="PLM74_04865"
     CDS             2210..3607
                     /locus_tag="PLM74_04865"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HON42450.1"
                     /translation="MLTLKEFLAKEVVPALGCTEPGAVALATARAREELGAEQVERIS
                     VRVSDSIYKNGMNVGVPGASGETGNALAAALGALAGKADLSLEVLRDCTPAHVSIAKS
                     MLGEGRVEVVCLPDVHGVFVEATVKSASGTATCVIEGEHTNIVSVSVDGKAIFRKGRR
                     EHGQCHGRGQDQDQDQTAEEGEGTAVGECGIDDSDIGDRDIMDGVRYQDLLELADHMA
                     PEEIDYVMRGVDMNKSIAEYGLASESLSGLGAGKAIWALMSDNKIEKNLSFLIRSYSS
                     AASDARMAGAQLPTMSAAGSGNQGITAIIPVALVGESMGLPREAIARGVTVSLLSTSF
                     VRSRIGRLTPVCGCSVAAGAGAAAGIAVLLGGNWVHAARAMQIVLSSTAGIVCDGAKG
                     TCALRVGAAACEAYLAALIAVNDRGINSAQGIVDDTIEGTADNVGRLNAEGMRDVDRV
                     LINIIEERVRRSEMM"
     gene            3613..4569
                     /locus_tag="PLM74_04870"
     CDS             3613..4569
                     /locus_tag="PLM74_04870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HON42451.1"
                     /translation="METRGQVFDIRRYSVNDGPGIRTAVFFKGCPARCAWCHNPESQE
                     HGREVMYWPSRCVACGACVEACPERAIRMEDGRPVVACGLCDVNVDGSACAVGAACVE
                     VCPAEARTIVGRTVSVADVMAEIERDRAFYDQSGGGVTFTGGEPLAQPEFLRALLEEC
                     RRHDVHSAVDTSGIVGARVMESIAPLVDLWLFDVKIVDPARHMLLVGCSNQPVLDNLR
                     FLAGRGSRIRVRVPLVPGINDGPDDLCDLARLLASLPGVRSGGPRQVDLLPYHRSGIE
                     KYARLGREYTLEGVEQAGAEYVESIAGELRSMGLDVRTGGGW"
     gene            4577..6943
                     /locus_tag="PLM74_04875"
     CDS             4577..6943
                     /locus_tag="PLM74_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014186229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HON42452.1"
                     /translation="MNARVSQLRHESLEAVPYISSERAELITQFYAGDRTASPVLRRA
                     SAFRHLMENKTIYIGNGELIVGERGPAPKAVPTYPELCCHSIGDLELLHSREKIPFAC
                     SPDVRKAYEERIIPFWRGRSMRDLIFDEMADEWKVAYEAGIFTEFMEQRAPGHTVADD
                     KIYRKGFKQLRQEIAASLADLDYSSDPKAQAKSEQLRAMDICAEAICIFARRHAELAR
                     EMAEQAEQDGDSARAAELRRIAKVCSWVPENPPRDFWEALQAYWFVHLGVITELNTWD
                     SFNPGRLDQHLYPFYQQGLECGTLTEESARELLQCFWIKFNNQPAPPKVGVTAAESGT
                     YTDFANINCGGLTPDGQDGVNDVTYLVLDVIDEMRLLQPSSNIQLSAKNPDRFLKRAL
                     RIVRKGWGQPSIFNADMVVQEMLRAGKSLEDARAGGTSGCVETGAFGKEAYILSGYFN
                     LPKILELALHDGMDPRTGRQLGPHTGHGSQLTSFDQLMTAFRQQLHHFIEIKVRGNNV
                     IERLYARYMPAPFLSLLVDDCISRGLDYHDGGARYNTTYIQGVGIGTITDSLSAIKRH
                     VFDGKHIGMGDLVAALDADFAGYERLRARLRDETPRYGNDDDDADAIMKSVFRAFFEE
                     VDGRPNTKGGCYCIDMLPTTCHVYFGSVTRATPDGRLAGEPVSEGISPVQGADRRGPT
                     AVLCSAAKMDHALTGGTLLNMKFTPRLLEGEEGIANLAHLVRTYFKLGGHHVQFNVVS
                     ARTLLDAQAHPESHRDLIVRVAGYSDYFCDLSRALQDEIIARTEHESF"
     gene            6965..7840
                     /locus_tag="PLM74_04880"
     CDS             6965..7840
                     /locus_tag="PLM74_04880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HON42453.1"
                     /translation="MLVDYHVHLERAGLSRENALRFIECAQARGLSEIAFTEHAYNFV
                     ECAPMLARPDYVSMHGHGFRIDEYVSLVENLKDEGFAVKLGIELDYVPETIDDAAAFL
                     SGRPFDLVIGSVHWIDEWGFDIEASSWNGVDVEWAYSRYFELACAAAASGVFDVIGHP
                     DVIKVFGARMGARIGEGCDEESSDVYGDSRGDSSRRLNGYYDALVSLAVASGTCLEVS
                     SAGLRRPVGEAYPAMDLLRLACGRGAAITLASDAHEPGEVGSRYSELVAWARAAGYGR
                     VTVFSGRQSLQVEIG"
     gene            complement(7964..>8456)
                     /locus_tag="PLM74_04885"
     CDS             complement(7964..>8456)
                     /locus_tag="PLM74_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HON42454.1"
                     /translation="FIYTIQPGDTFYLIALRYGTTVDALIRANPGVDPNRLFVGQRIC
                     VPVVAPPPVPARTCVLMLSPRITGSNAGGVLWLRTDQFGTTQILVGGVNLAELPVGQD
                     LYRAIFTWPGGTVTVPMTPVPGQAGVWVGTAVQALPAVAFTSGTVDVYPGPILGAFLR
                     DCR"
BASE COUNT         1647 a         2372 c         2717 g         1720 t
ORIGIN      
        1 atgcgaggag gctatggcct gatttcgtgt accttgatga ggtttgtagt gccggacaca
       61 cctaccggca ctccggcggt gattacaact gtgtcgccgg gccgtgcata acccgcttcc
      121 accgccttgt tcactgcaat gaggatcatc tcgtctgtcg tcgtgatgtc ttctcccagt
      181 atggggtaga cgccccacag caggttgagc gttcttgtag tgtgcatatc ggtggctact
      241 gctatgatca tcgtccgggg cctgtgtttg gagatcatcc gggccgtcca tcccgacttg
      301 gtggaagtga tgatggccgc ggctctgagt tcagcggcta tttgcactgt agcatgacct
      361 atagcgtcgg ttaccggcat cgacgcgctg cccattcgcg gggttgcggc atccgtgtac
      421 tgcagtgcca tttcggctcg ctcagctatg cgggccatcg tgcgaaccga ctctacagga
      481 tacatcccgc tggcagtctc accgctcagc atgacagcgt cggtaccgtc gaataccgca
      541 ttggctacat cgctcacctc ggccctagtg gggctggggt ttctgatcat cgaatcaagc
      601 atctgtgtgg cagtgatcac cggtttgccg gctctgcggc acttctgtat catctgtttc
      661 tgtatgatgg gcacgtcttc cgcgggcatt tcgacgccca ggtcgccccg ggccaccatg
      721 agaccatcag cagcgcgtag tatgctatca agactctcga tgccctcacg actctcgatc
      781 ttggctatca ccggaatgct ggaaccatgc tctgcaagga atgatttgac ttgctgtaga
      841 tcggctgcgc tccgcatgaa ggacgcagcg ataaggtcaa caccgttttc tatgccgaag
      901 aggagatcgt ctttgtcatc tgcggacagt gtagggaggt tgagccgccg accgggtatg
      961 gatatccgct ttctgtcgct cagctgcccc ccgacaacta ccttgcattc aacggcctgg
     1021 ccctgcacag ctgtgacttc cagctcaagc agtccatcgt ccagcagaag acggtcattg
     1081 ggcgcaacga taagcggtag gcccgggtag ttcacagaca ccacattagg cccgcccatg
     1141 accggttccg tggtgatcgt gaatgacgca ccgggctcaa gcgtgatcgg ccctgaggcg
     1201 aatcggccga tccttagctc agggcctttt gtgtctagca tgatcgggat gtagcgatga
     1261 tactcggcct ctagctcgcg tatcatgcgt atccgctcag cgtgttcagc gtgagtgcca
     1321 tgggagaaat tgagacgggc cacgtccatg cctgcttcaa ttagctttcg cagtgaatcc
     1381 agcccatggc tggcaggtcc tattgtgcat acgatcttgg tctttctcaa cagctgcgcc
     1441 tccgtcagtc ttcgcttacc ggccttccgc atgccacgca gaaccgtgcc ccttccagca
     1501 cgagagtgcc gcactcgcag gttcttaggg gcttgggctc ttcgaggaca gagggttcac
     1561 catcacaagc agggctttcg tcggtgcttt ccggcacgtc gacctccgcc ggttgtgcgg
     1621 gcacgtcttc agcgtccgct tgttgctgtt ccctcgacgc ttcccggatc ctctggatct
     1681 cttcctccgc cgcggcgatc tggcgatcga tttcatggat tgcgtctgcc ttggcacgca
     1741 acaccggctc tgccagcgac ccccctgata gcatttcgaa ggtcagtcgc cccaggtctt
     1801 caaggagatc acgccgcttg gccttgagat cgctgattct ggcgcggatc acggtggtgt
     1861 cccacaactc cttggacttg gctcccacag tgtccacacc aaccccgatg gtcttcttaa
     1921 tcttgtcgaa tatgtcggac acaggcgccc acctcccgtc tttgctatca ccttcaatat
     1981 ttcgcctcat ttcctctatc tcctccattc ccgacgactt gcgcatgagc cgcaacacag
     2041 gatacgtatc gatcacatac agtatgctct gcctgccatc acatcgtgcc ggccggcgta
     2101 tcattgcata gagggaaatc gaaagcggat caagaatcag tcaaggtgat aatgaccttc
     2161 gctgtgtgtg ttgagaaggc gcagaatgcg tgagggggca gggcctgcca tgctgacact
     2221 gaaggagttt cttgcgaagg aagtagtccc ggcacttggg tgtacggagc ccggcgcggt
     2281 cgcgctggcg acggcccggg cgcgggaaga actaggtgct gagcaggtag agaggatatc
     2341 ggtcagggtt agcgatagca tatacaagaa cgggatgaat gtgggggtcc cgggcgcatc
     2401 gggcgagacc ggcaacgcgc ttgccgccgc ccttggcgcg ctggcgggca aggcggatct
     2461 gagcctggaa gtgctgcgcg actgtactcc ggcccacgtg agcatcgcca agtctatgct
     2521 gggcgagggc agggtggagg tcgtgtgtct gcctgatgtg catggtgtgt tcgtcgaggc
     2581 gaccgtgaaa tccgcgtcgg gcactgctac gtgtgtaatc gaaggcgagc ataccaacat
     2641 agtcagcgta agcgtggacg gaaaggcgat ctttcgcaaa ggacgccggg aacacggcca
     2701 gtgtcatggc cggggccagg accaggacca ggaccagact gccgaggaag gcgagggcac
     2761 tgcggtcggc gaatgcggca ttgacgacag cgatattgga gaccgcgata tcatggacgg
     2821 cgtcagatac caggacttgc tcgaactggc cgaccacatg gcgcccgaag agatcgacta
     2881 tgtgatgcgc ggtgtggata tgaacaagtc tatcgccgag tacgggttgg cgtccgaatc
     2941 cctgtcaggc ttgggggccg gcaaggcgat ctgggcactg atgtccgata acaagatcga
     3001 gaagaacctg tccttcctga tcaggtcata ctcgagcgct gcgtctgatg ccaggatggc
     3061 cggggcccag ctgcccacca tgagcgctgc cggcagcggc aaccagggca tcacagctat
     3121 catccccgtt gcgttggttg gcgaatctat ggggttgccg agagaggcta ttgccagagg
     3181 cgtgacggtg agcctgctct ccacgagctt cgtgcgcagc cggatcggcc gcctaacccc
     3241 tgtgtgcgga tgctcggttg cagccggcgc cggtgcggcc gcagggatag ccgtgctgct
     3301 cggaggcaac tgggtccacg cggcgcgagc catgcagatt gtgctttcta gcactgccgg
     3361 catagtgtgc gacggggcca agggcacttg cgcgctgcgc gtcggcgccg cagcctgtga
     3421 agcgtatctg gctgccctga tcgccgtgaa cgaccggggc atcaattcgg ctcagggcat
     3481 tgtggatgat acgatagagg gaacagccga caacgtgggc aggttgaacg ctgagggcat
     3541 gagagatgtt gaccgagtgc tcattaacat aattgaggaa cgtgtacgaa gaagtgagat
     3601 gatgtagcga cggtggagac gcgcggacaa gtattcgaca ttcgccgcta ttcagtcaat
     3661 gacgggccgg gaatccggac ggcggtcttc ttcaagggat gtcctgcccg gtgcgcgtgg
     3721 tgccacaacc ccgagagtca ggagcacgga cgcgaggtca tgtactggcc gtctaggtgt
     3781 gtggcgtgcg gggcgtgtgt tgaagcgtgc ccggagcgtg ccatacggat ggaggatggc
     3841 cgacccgtgg tggcctgcgg gctatgcgac gtgaacgtgg acggttcggc gtgcgccgtg
     3901 ggtgccgcgt gcgtggaggt gtgccctgcc gaggcgcgca cgattgtggg acggactgtg
     3961 agtgtggccg atgtcatggc tgaaatcgag cgggacaggg cgttctatga tcaatccggc
     4021 gggggagtaa cgttcacagg aggcgaaccc ctagcgcaac cggagtttct gcgcgctttg
     4081 ctcgaggagt gccggcggca tgacgtgcat tctgcagtcg ataccagcgg cattgtcggc
     4141 gctcgggtga tggagtccat tgcgcctctg gtggatctgt ggctgttcga cgtgaagatc
     4201 gtcgaccctg ctcggcatat gttgctggtg ggctgctcca accagcctgt gctggacaac
     4261 cttaggttcc ttgctggtcg gggcagccgg attcgggtgc gcgttcctct ggtacccggg
     4321 ataaacgacg gtccagacga tctgtgtgac ctagcccgtt tgctggcatc actgcccggt
     4381 gtgcgcagcg gcgggcctcg tcaggtggat cttctgccct atcaccggag cggaatcgag
     4441 aaatacgcca ggctgggcag ggagtacacg ctcgaaggcg tagagcaggc aggcgccgaa
     4501 tatgttgagt cgattgccgg cgaattgcgg agtatgggtc tagatgtccg aacaggtgga
     4561 ggatggtgag cggtcaatga acgcccgtgt cagtcagcta cgtcacgaga gccttgaggc
     4621 ggtcccgtac atctcatcgg agcgtgcaga gctcattaca cagttttatg caggcgatcg
     4681 cacggcctcg ccggtgctgc gccgagccag cgccttcagg cacctcatgg agaacaagac
     4741 gatatacata ggaaacggcg agctgatcgt gggggaacga gggcctgcgc ccaaggccgt
     4801 gccgacctac cccgagctat gctgccattc cattggtgat cttgagttgc tgcatagcag
     4861 ggagaagata ccgtttgcat gctcccctga tgtccggaag gcatatgaag aaagaataat
     4921 ccccttctgg aggggccgtt ccatgcgtga tctcatcttt gatgagatgg ccgacgagtg
     4981 gaaggtcgcc tacgaggcgg ggatcttcac agagttcatg gagcagcgtg cgcccggcca
     5041 cactgtggca gacgacaaga tctaccgtaa ggggtttaag cagctgaggc aggagatcgc
     5101 ggcaagcctc gctgacctgg actactcgag tgatcccaaa gcgcaggcga aatcggagca
     5161 attgcgcgcc atggatatct gcgccgaggc gatctgcatt ttcgccaggc gccacgcaga
     5221 gcttgcgcgg gaaatggctg agcaggcaga gcaagacggc gactccgccc gcgccgctga
     5281 gctccggcga atcgctaagg tctgctcatg ggtaccggaa aacccgccca gggacttctg
     5341 ggaggcgctt caggcctact ggttcgtgca tcttggcgtc ataactgagc tgaatacatg
     5401 ggactcattc aacccgggcc gtcttgacca gcatctgtac ccattctacc agcaaggatt
     5461 ggaatgcggt acgctgacag aggagagcgc ccgcgagctt ctgcaatgct tctggatcaa
     5521 gttcaacaac cagcctgcgc cgcccaaggt gggagtgact gccgccgaga gcggcacata
     5581 caccgacttt gccaacatca actgcggcgg gctgacgcct gacgggcagg acggcgtgaa
     5641 tgatgtcacc tatcttgtgc tcgacgtgat cgatgagatg aggctgttgc aacccagttc
     5701 caatatacag ctgtcggcga agaaccccga ccgcttcctc aaacgggcac tacgcattgt
     5761 gaggaaaggc tgggggcagc catcgatctt caatgccgac atggtcgtgc aggagatgct
     5821 gagggcaggc aagagcctgg aggatgcgag ggcaggcggc acgtcagggt gtgtagagac
     5881 cggcgctttc ggcaaggagg cctacatcct ttcggggtac ttcaacctgc cgaagattct
     5941 ggaactggca ttgcacgacg gcatggaccc ccggaccggc aggcagctgg gcccacacac
     6001 tggccatggg agccagctta cgtcatttga ccagctcatg accgcatttc ggcagcaact
     6061 tcatcatttc atagagatca aagtgcgcgg gaacaacgtc atagagcggt tgtatgccag
     6121 gtacatgccg gcgccgttcc tttcgctgct ggtggatgac tgtatctccc gcggcctcga
     6181 ttaccacgac ggcggagctc gttacaacac cacctatatt cagggagtag ggatcggcac
     6241 catcaccgac agtctgtcag cgataaagcg acacgtgttc gacggtaagc atatcgggat
     6301 gggcgatctc gtggcggcgc ttgacgcgga tttcgcgggt tacgagaggc tgcgggcacg
     6361 actgcgcgat gaaaccccgc gttacggcaa tgacgacgat gacgctgacg ctatcatgaa
     6421 gtcggtgttc cgagcgttct ttgaggaagt ggacggccgc cccaacacta agggggggtg
     6481 ctactgcatc gacatgctgc ctaccacatg ccacgtctat ttcggcagcg ttacgcgggc
     6541 cacgcccgac ggaaggctcg ccggtgagcc tgtgtcggaa gggatctcgc cggtgcaggg
     6601 ggccgacaga cgggggccta ctgcagtgct gtgctcggcg gccaagatgg accacgccct
     6661 cacagggggc acgcttttga atatgaagtt cacgccgcga ctattggagg gcgaggaggg
     6721 catcgcgaac ctcgctcacc tggtgcgcac gtatttcaaa ctgggcggcc atcacgttca
     6781 gttcaatgtg gtcagcgcca gaacactgct cgacgcgcag gcgcacccgg agagccaccg
     6841 cgaccttatc gtgagggttg ccggatacag cgactacttc tgcgatctgt cgcgcgctct
     6901 gcaagatgag attatcgcac gaacggagca cgagtcgttc taggcaatcg gggtgagtca
     6961 cgctatgctg gtcgactatc acgtccatct ggagagggcg gggctatcgc gggaaaacgc
     7021 cctgcgtttc attgaatgcg cacaggctcg cgggctgagc gagatcgcat tcactgagca
     7081 cgcgtacaac ttcgtcgagt gcgcgcccat gcttgctcgg cccgactacg tgtcgatgca
     7141 cggccacggg ttcagaatcg acgagtatgt gtcgcttgtg gagaatctga aagacgaagg
     7201 tttcgcggtg aagctgggaa tcgagctaga ttacgtgcct gagacaattg acgatgccgc
     7261 cgcctttctc agcgggcgcc cgttcgacct ggtgatcggg tccgtccact ggatcgatga
     7321 gtggggtttc gatatagaag cgtccagctg gaatggcgtg gatgtggagt gggcgtactc
     7381 tcggtacttc gagttggcat gcgccgcggc cgcaagcgga gtcttcgacg tgatcggcca
     7441 cccggacgta atcaaggtat ttggtgcgcg aatgggtgcg cgaatcggcg aagggtgtga
     7501 cgaggagtcc agcgatgtct acggcgacag ccgcggcgat agcagccgga gactgaacgg
     7561 ctactacgat gctttggtgt cactggctgt cgcgtcggga acatgccttg aggtgtcaag
     7621 cgccggcctg aggcgccctg tgggagaggc gtatcctgcc atggatcttc tgcgactggc
     7681 ctgcgggaga ggcgcggcaa ttaccctggc atccgatgcc cacgagcccg gggaggtggg
     7741 ttcgcgctac agcgagctag tcgcgtgggc acgggctgcc ggctatggca gagtcaccgt
     7801 tttcagcggg cgccagtcgc tgcaggttga gatcggctag aagctgagat ggagtagatg
     7861 gagtggaaaa ggaggccggc ccgcgtgggc cggccgagtg gcgtcttgtc ctgaactact
     7921 ctccgagtcc tctctcggta ctgcgactct gttgcgggcg cgcctatctg cagtctctca
     7981 ggaacgctcc cagtatgggg ccggggtaga cgtccactgt acccgatgtg aacgcaactg
     8041 ccggcagagc ctgcaccgcg gttccgaccc acacgcccgc ctggcccgga acgggcgtca
     8101 tgggcacggt cacagttccg cccggccatg tgaagattgc tctgtacaga tcctgcccca
     8161 caggcagctc ggccaaattg acacccccca ccaggatttg cgtggtgccg aactggtccg
     8221 tgcgcagcca cagcacaccg cctgcgttgg agcccgtaat cctgggcgac aacatcagca
     8281 cgcaggtgcg tgcaggtaca ggaggcggcg cgacaaccgg cacgcagatc ctttgaccca
     8341 cgaagagcct gttcggatcg acaccggggt ttgccctgat tagagcgtct acggtcgtac
     8401 catacctcaa ggcgattagg tagaatgtgt caccgggctg gatcgtgtag atgaac
//
LOCUS       DAOTAC010000081         8414 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_7197_length_8414_cov_4.743510, whole genome shotgun sequence.
ACCESSION   DAOTAC010000081 DAOTAC010000000
VERSION     DAOTAC010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 8414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8414
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7197_length_8414_cov_4.743510"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1273..2526
                     /gene="mltG"
                     /locus_tag="PLM74_04890"
     CDS             1273..2526
                     /gene="mltG"
                     /locus_tag="PLM74_04890"
                     /inference="COORDINATES: protein motif:HMM:NF014656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HON42455.1"
                     /translation="MAETGTNEARNRRGVGVSGRGTSEAGRSEAGRPRGRLGVTGAAV
                     LVLAAVVLAATGLALLGVSGWFVPGPRGERVVVSIPDRTPASQIAAILQREGLIGSER
                     LFKLAVRASGKASSLKSGQYEFVRGDSMISIIERISLGQVMTIKVTVPEGLTLRQVAK
                     LMGDRSVAAEDDMLAVASDPPEQMRADFPFIREGVSLEGYLFPDTYEFASGVGAMKVI
                     RTMLRRFENTAQVALDHVALDQATLDQATPDQATLDQAPPDSASRAEAGPPTLDDELP
                     NGLTQRETLILASIVEREARVESERPIIARVFLNRLERSMPLQSCATVQYILPESKEH
                     LLHEDLEIESPYNTYLVAGLPPGPICSPGLASIRAVLDPEPSDYLYFVASNDGSHVFS
                     RTYKDHLQAKARIEREARLGRQAGR"
     gene            2552..3166
                     /gene="udk"
                     /locus_tag="PLM74_04895"
     CDS             2552..3166
                     /gene="udk"
                     /locus_tag="PLM74_04895"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020754577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HON42456.1"
                     /translation="MARDVLVVGIAGGSGSGKTTVVRAISEAFGNAVAVLEHDAYYRD
                     QSCLSLDERLKTNYDHPFAFDTDLYIDHARQLVSGLAVDRPVYSFESYTRTSDTVCVR
                     PAGVLILEGILVLEDARIRELMDIKAFVDADPDVRFIRRLMRDVQERGRTLDSVVDQY
                     LNVVRPMHLQFVEPTKRYADVIIPEGGFNRVAIELLIARLKSAV"
     gene            3326..5029
                     /locus_tag="PLM74_04900"
     CDS             3326..5029
                     /locus_tag="PLM74_04900"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HON42457.1"
                     /translation="MNHRPSRGSAGPAVVQAAIIMCFLVLLGRCAWIALVHGPDYAVR
                     AVQQRSVDVVVERVRGQILDRDMHPLAGTSDGLASSWPHRAVVVVFPSLVSGPDDALS
                     RLDELIPGQGVCAGSARAVSLSQAACTEVEAMAGVVRGIVPLSLPSRYLGLASHVIGG
                     IEISYDWALRGGDGREVVRLMCDACGREIPGLGARRVALGQPTGWVRLAVDSGLQACV
                     EKALDSRAPGMAGAVVAMDPWTGQVLAMASRPFYDPDDVAGYLTRPDAPMINRAIRPY
                     YPGSVFKVIIAAAALSTGARSPESVYTDTGSIEAGGHTFSCHARERGGHGDITFQDAL
                     IHSCNTSFIDTAMQLGAQPILDFARALGISDPDVASAVRLPGSITGALPHSRTRLGPA
                     DLANLALGQSLVQITPLEAAAMMCAVANGGVWHRPELVLEACGPDGRRVTRVASVASM
                     PQQAEGAGQGQGDRVLRADVAATLREMLAGVASRGTASAALGGMDVAGKTGTAQTGRV
                     DGSGASIFNAWFVGFAPVESPRLVVAVLVEGPESGAAAAAPIFGDICEYATGAARGRA
                     R"
     gene            5082..5780
                     /gene="sigK"
                     /locus_tag="PLM74_04905"
     CDS             5082..5780
                     /gene="sigK"
                     /locus_tag="PLM74_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013843081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigK"
                     /protein_id="HON42458.1"
                     /translation="MVASGLLALASLLARGIMLLHAHVSGGGAFPPPLSEEEEREALE
                     RKEAGDESARAVLVERNLRLVAHVVKKYDVVGEDTDDLISIGTIGLIKAINTFDRHKK
                     TRLATYAARCIENEILMHLRSGRKSRGELSLYDPIGSDKEGNEIVLMDVLTQDGDTVA
                     EMVESLVEQERLARKLAELTPRERRVLELRFGMGEFARQTQREISKALGISRSYVSRI
                     EKRALAKLQKEMYR"
     gene            5847..6362
                     /locus_tag="PLM74_04910"
     CDS             5847..6362
                     /locus_tag="PLM74_04910"
                     /inference="COORDINATES: protein motif:HMM:TIGR01668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HON42459.1"
                     /translation="MNRGERRKTALIQPDMRVDGVWDIDLDELSRRGIAGIIVDIDNT
                     IAPWGTYDLSERAAEWLERARNRGFGVCLLTNNHRSRADWFARALGLPAVCGWVKPWR
                     WGYRRAMHILGTRPDSTVMVGDQLFTDILGAKRLGIHAILVSPLGDKELPHTRFKRRL
                     ERFLLGRKGDS"
     gene            6362..>8414
                     /gene="priA"
                     /locus_tag="PLM74_04915"
     CDS             6362..>8414
                     /gene="priA"
                     /locus_tag="PLM74_04915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HON42460.1"
                     /translation="MTGRYAQVVVDVPVRRVDRHYDYLIPGRLEARVEPGSRVLVPFG
                     ARSVEGFVISRSAQPAVDNVREIIDCVTDAPGVPRELLQVAEWMADTYVCLLIEALRA
                     MVPSGTRVESSVVVRLVGSEDDALRAKIDGVARKVFDYLAECGGSAARDEIAAALEIA
                     SVSRHVATLTRAGLVEVDTEWERPKVRARRVRAFQLAPSAGDPGATARRLAGSSPAQA
                     AIMQVFASTLPRAVDGGSDGANTDGADARTGASAIPRAELLKRAGCSASALDALVRAG
                     LLHPTEVVASRSPMEGWSAGRASVPVPVPAPNLTVDQERAVERVTARMDSGGGIVVLH
                     GVTSSGKTEVYLRAFEHALARGRRGILLVPDIALTPQMVDGVVARFGPERVAVLHSAL
                     GAGERLDEWERARRGQVDIVVGARSAVFAPVPSLGLIVVDEEHDGSYKQDESPRYHAR
                     DVAVFRAKRAGATVVLGSATPSLETYSKAESGEYELIELPRRIDDRPMPDVAVVDMRS
                     ELKAGNRTIFSRLLRARIEQTLERGQQAILFMNRRGFSTFVLCRECGHVMTCPHCDVS
                     LTLHGSGSRRSELVCHYCNHTERAPDVCPSCGGIHIRYFGAGTERIEDEVRKSFPKAR
                     VARMDVDTTRRKGAHRAILGAFRAGRTDILVGTQMIAKGLDFPNVTLVGVVSADTALN
                     LP"
BASE COUNT         1594 a         2407 c         2776 g         1637 t
ORIGIN      
        1 ttttccgaca ggcctttggc ggagcctgtc ggaataccct acggttctga acgcactggc
       61 aatcctgatc gcaacgggct ctggcgcttc ctttcgcagg cggtcagcat taggtaaatg
      121 tgtagctaga gtccgtgtca gagtgcggcg tttcgatggt gggccgccag ggactgctat
      181 ccggggatct acgatcccca gaacgccctc agatttcgcc cgaccgcgct gaaccgtacg
      241 gtattccacc aggaaggcaa aaagaccatg gggttttgcc acaggccaga cagagccgtg
      301 cccaactcga ctttagcgac ttggggcctg ttgctcccga ggctacacga cactgcaaag
      361 tggatcggct cgctagtttg gcacctcaac tggcgggccg ggataaacct gacgcctcca
      421 gccggtgcac ttcccgcctt ggcattcggc gtcccgctca acccatccaa ctccggattt
      481 ggctttttgg catgcggcct cgccgggacc acctcccggg agcacctcgc cgggagcacc
      541 tcgaaagaga ctggggtaga aagggcccga agatgcatcg gcggacagat ccgggtacaa
      601 aaaggcgatc accacagctc aacggtatac ccccgggggt atccgagcag gcatgccgtc
      661 agtttttgcc gatagttttg ccgataggcg caatttttga gtacatgcat ttggatcggg
      721 agccgccggc cggcaaattg cgctgagaag ggcgtttccg aggtcggcga tagcctatta
      781 tttggtgaac cagcggaagc gaatgccact gttcacgcat aatcgccaaa tacgtccaag
      841 tcatgggaca tcctgtcacc ttcgttgccc ggttccatgc ccggaccagt cggatacccc
      901 ccgattttcg tgatttggag gcggtcccgc agtcccccga aatcgatgta ctcaaaaatt
      961 gccggtatct caaaaactat tgccgaaaac tgacgatata ccagttccaa gccgttttga
     1021 cccactaccg tgacagttat accagctaaa acgccatttt ggcgaggatg cctgcttcca
     1081 ccgtcgctcc gtctggcaat gctcgctagt acctccactt cgcgacacgt acatgaacta
     1141 cgcagatccc acgcgctggc caccacacgt tggccacacg ttggccttcc ttgcgccgat
     1201 ctctccagcg acctataatg ggtaatggtt catagcgctt cccagagggc ggatggcgag
     1261 gtggagggac ttatggctga gaccggcacg aacgaggccc gaaatcggcg cggcgtagga
     1321 gtatccggac ggggcacatc cgaggcaggc cgatccgagg caggccgacc caggggcagg
     1381 ttgggcgtga cgggcgcggc agttcttgtt ctggccgcgg tggttctggc agcgacgggg
     1441 ttggctctgt taggcgttag tgggtggttt gtgcccgggc ctcgtggcga gagagtagtt
     1501 gtgtccatac cggaccggac gccggcttcg cagattgcgg caattctcca gcgcgagggg
     1561 ctgatcggtt cagaacgcct gttcaaactg gcggtgcgag catccgggaa ggcgtcttcg
     1621 ctcaagtccg gccagtacga gtttgtgcgc ggcgacagca tgatatcgat aatcgagcgg
     1681 atatcattgg gccaggtaat gacgattaag gtcacggtac ccgaggggct gacgttgcga
     1741 caagtcgcaa agcttatggg cgatcgcagc gtggctgcgg aggacgacat gctcgcggtg
     1801 gccagcgatc cgccggagca aatgcgcgcc gatttcccgt tcatccgtga aggcgtgtcg
     1861 cttgaaggct acctctttcc ggacacgtat gagtttgcca gcggggtagg ggcgatgaag
     1921 gtcattcgga ccatgttgcg gcggttcgag aacacagcgc aggttgctct ggatcacgtg
     1981 gctctggatc aggcgactct ggatcaggcg actccggatc aggcgactct ggatcaggcg
     2041 ccgccggact ccgcgtctag ggccgaggcc ggtccgccga ctctggacga tgagctgcca
     2101 aacggcctca cacagcggga gactctaatt ctcgcttcga tcgtggagcg ggaagccagg
     2161 gttgagtccg aacggcccat cattgccagg gtcttcttga accggctcga gcgttcaatg
     2221 ccgctgcaga gctgcgcgac tgttcagtac attctgccgg agtccaagga gcaccttctg
     2281 cacgaagacc tggagataga gtcgccatac aacacgtatt tagtggctgg actgcccccg
     2341 ggacccatat gcagcccagg cctggcatcg atccgagcgg tgctcgaccc ggagccaagc
     2401 gactatctct atttcgtggc gtccaatgat ggaagccatg tgttcagccg cacctacaag
     2461 gaccacctgc aggccaaggc caggatcgag agagaagcca ggctgggtag acaggcaggc
     2521 agataaggaa gaaccagggg gtagacactc gatggccaga gacgtacttg tcgtcggcat
     2581 agccggcggt tcgggatcag gcaagaccac ggtggtgagg gctatatccg aagcgttcgg
     2641 aaacgccgtg gccgtgctgg agcatgacgc ctactatcgg gatcagagct gcctcagtct
     2701 cgatgaaaga ctgaagacca actacgacca cccgttcgct ttcgataccg acctttacat
     2761 cgatcacgcg aggcagctcg tcagcgggct agcagtggat cggcctgtct actccttcga
     2821 gtcatatact cgcacttccg atacggtatg cgtaaggcct gccggtgttc tgatactcga
     2881 gggaattcta gtccttgagg acgcgcgcat tcgggagttg atggatatca aggctttcgt
     2941 cgacgccgac ccggacgtgc gcttcattcg caggctgatg cgggatgtcc aggagcgggg
     3001 caggactctt gattcagttg tggaccagta tttgaatgtc gtgcgcccca tgcatctgca
     3061 gttcgtggaa cccaccaaga gatacgccga tgtgatcatc cccgagggcg gcttcaacag
     3121 agtggcgatc gaactcctca tcgcccgtct aaagtcggca gtgtaggccc gtgcccgcgc
     3181 gggcgccgtc tgtccattcc ggtcgtgtcg cccaggcagc cttctgccaa gaacaccttc
     3241 ggcgccgcga aagctccggg ccgccggcgg gcagaatact gctgggaatc gcgcacgcgc
     3301 cgtgcagcag cggaggggct gccgcttgaa ccaccgtccg tctcgcgggt ccgcagggcc
     3361 tgccgtggtg caggccgcca taatcatgtg ttttctcgtc cttctcgggc ggtgcgcctg
     3421 gattgcgctg gtgcacggcc ccgactatgc cgtgcgcgcc gtacagcagc gctcagtgga
     3481 tgttgtggta gaacgcgtcc gtgggcagat tctcgataga gacatgcacc ctctcgcggg
     3541 cacgtcggac ggactcgctt cgtcatggcc gcacagggct gttgtcgtag tcttcccgtc
     3601 tcttgtttca gggccagacg atgctctgag tcgcctggac gaactgatcc caggtcaggg
     3661 cgtatgcgct ggaagtgcgc gtgcagtcag tctttcgcaa gccgcttgca ccgaggttga
     3721 ggcgatggcc ggcgttgtgc gaggaattgt tcctctatca ctcccatcac gctacttggg
     3781 gctggcgtcg catgtgatcg ggggtatcga gatctcatac gactgggcac taaggggcgg
     3841 agacggccgc gaggtcgtgc ggctcatgtg cgatgcatgt gggcgcgaga taccggggct
     3901 cggagcgcgc agggtggccc tggggcagcc cacaggttgg gtgagattgg cagtggattc
     3961 gggccttcag gcctgcgtcg aaaaggctct ggatagccgg gcgcctggta tggctggagc
     4021 ggtggtggcc atggatccct ggacggggca ggtgctcgcc atggcgtcgc ggccgttcta
     4081 tgacccagat gatgtggcag gctaccttac gaggcccgac gcacccatga tcaaccgagc
     4141 catccgtccg tactatccgg gctcggtctt caaggtcatc atagcggcgg cggccctgtc
     4201 tacaggggcc aggagccctg agtctgtata cacggacacc ggcagcatag aagctggcgg
     4261 acatactttc agttgccacg cgcgggaacg cggcgggcac ggagacatca ctttccagga
     4321 tgccctcatc cattcgtgca acacttcgtt catagatacg gccatgcagc tcggtgccca
     4381 acccatactg gacttcgccc gcgcgctggg aatcagcgat cctgacgtgg caagtgcggt
     4441 gcgactccca ggcagcatca cgggtgcact gccgcacagc cgaacccgcc ttggcccggc
     4501 tgatctagcc aacctggctc tcgggcaatc gctcgtgcag atcactccgc tcgaggctgc
     4561 ggctatgatg tgcgccgtgg cgaatggagg tgtgtggcac cgccctgagc tggtgctcga
     4621 agcatgtggg cctgacggcc ggcgcgtgac ccgcgtggcg tctgtggcgt cgatgccgca
     4681 gcaggcagag ggggctgggc aggggcaagg agaccgcgtg cttcgcgctg atgtcgccgc
     4741 cacgctccgg gagatgctgg caggggtggc gtcgcgcggg actgccagcg ctgcgctggg
     4801 cggcatggat gtggccggga agaccggcac tgcgcagacc gggcgggtgg atgggtcagg
     4861 ggcgtcgatt ttcaacgcct ggttcgtggg gtttgctcca gttgagtcgc cccggctggt
     4921 agtggccgtc ctggttgaag ggcctgagtc aggcgccgcc gctgccgcgc cgattttcgg
     4981 agacatttgt gaatatgcca caggcgcggc acgcggacgt gcacgatgaa gccatccgtc
     5041 tcataagctg agtctgtatc acaagacgga ggtggcgcat tgtggtcgca agcggcctgc
     5101 ttgctctggc gtctttgctg gccaggggca tcatgctcct gcacgcgcac gtctcggggg
     5161 gcggcgcatt tccgccgccg ctgtcggaag aggaggagcg ggaggcgctg gagcggaagg
     5221 aggctggcga cgagtccgcc cgcgctgtgc tggtcgagag gaatctgcga ctcgtagcgc
     5281 atgtggtcaa gaaatacgac gtggtgggag aggataccga cgacctgatc tcgataggca
     5341 ccatcgggct gatcaaagcg ataaacacct tcgaccggca caagaagaca aggcttgcga
     5401 catacgcggc gcgctgcatc gaaaacgaga ttctcatgca tttgcgatcc gggcggaaaa
     5461 gccggggcga gctgtcgctg tacgatccca ttggatccga taaggaaggc aacgagatcg
     5521 tgctgatgga tgtgcttact caggatggcg ataccgtggc ggagatggtg gaatccctgg
     5581 tggagcagga gcgcctcgcc cggaaactgg cggagctaac gcccagagaa cggcgtgtct
     5641 tggaactccg gtttggcatg ggcgagttcg cccgccagac tcagcgggag atctccaagg
     5701 cgctcggaat ttccaggtcg tatgtgtcgc ggatagagaa gcgagctctg gcgaaactgc
     5761 agaaggagat gtacaggtag aggacgatac ggcagtcttc atccactcaa gcacgctcac
     5821 gcagacacgc tgggatacgt ggtataatga accgtggtga gaggaggaag acagccctga
     5881 ttcagccaga catgcgtgta gacggcgtgt gggacataga cctggacgag ctgtcaaggc
     5941 gtggcatcgc aggcataatc gttgatatcg acaacacgat tgccccatgg ggaacttacg
     6001 acctttccga gcgcgccgcc gaatggctcg aaagggccag gaaccgcggg ttcggcgtct
     6061 gcctgctcac caacaaccac cgttcgcgcg ccgactggtt cgcccgcgcc ctcgggctgc
     6121 ctgcggtgtg cgggtgggtc aaaccatggc ggtggggata ccgccgtgcc atgcatatcc
     6181 tgggcactcg tccggattct acagtaatgg tgggcgacca gctgttcact gatatcctgg
     6241 gcgcaaaacg tctgggaata catgcaatcc tggtatcgcc tctcggcgac aaggaacttc
     6301 cccacacccg tttcaaacgg cggcttgagc gattcctcct gggcaggaag ggcgatagct
     6361 gatgacggga aggtacgctc aagtcgtagt ggatgtgccc gttcgtcggg tagacaggca
     6421 ctacgattac ctcatcccgg gccgtctcga agcccgcgta gaaccgggtt cgcgtgttct
     6481 ggtcccgttc ggagcacgaa gtgtagaggg gttcgtaata tcgcggtcgg ctcagcctgc
     6541 agtggacaat gtgcgggaga tcattgattg cgtgacagac gcccccggag tgccccgcga
     6601 actgctgcag gtggcggaat ggatggctga tacatacgtc tgcctgctga ttgaggccct
     6661 acgagccatg gtgccgtcag gcacccgggt tgagtcttca gtcgtggtcc gtcttgtggg
     6721 ctctgaagac gatgctctgc gggccaagat agacggcgtg gcgcgcaagg tcttcgacta
     6781 tctggcggag tgcgggggca gtgccgcgcg ggacgagatc gccgcagctt tggagattgc
     6841 atcggtgtcg cgccatgtgg ctactctgac ccgggcaggg ttggtcgagg tagacacgga
     6901 atgggaacgc ccgaaggtcc gcgcgcgtcg cgtgagagca ttccaattag ctccgtcagc
     6961 aggcgaccct ggcgccacag ctcggcgact agccggctcc agcccggcgc aggctgcgat
     7021 aatgcaggtc ttcgcgtcga cgctgccccg agcggtggac ggcggttctg atggagccaa
     7081 tactgacgga gccgatgccc gtaccggcgc gagcgccatc cccagggctg aactactcaa
     7141 acgtgcaggt tgctctgcgt cggccctaga tgccctggtg cgtgcggggt tgcttcatcc
     7201 tactgaagtc gttgcatctc gatcgccaat ggaagggtgg tcagcgggcc gcgcgtctgt
     7261 gcccgtgcct gtgcctgcac ctaatctaac ggtcgatcag gagcgcgcgg tggaacgtgt
     7321 tacggcacgc atggattccg gcggcgggat cgtggtgctg cacggggtga ctagctccgg
     7381 caagaccgaa gtctacctgc gagctttcga acatgcgctg gcccgcggca ggcgcgggat
     7441 actgctggtc ccggacatag cgctcactcc ccagatggtg gacggggtgg tggcaaggtt
     7501 cggccccgag cgtgtggccg tgcttcacag cgcccttggc gcaggcgagc gcctggacga
     7561 gtgggagcgc gcgcgcaggg gccaggtgga catcgtggtg ggggccaggt ccgcggtatt
     7621 tgcgcctgtg ccgtcgctgg ggctgatagt ggttgacgag gaacacgatg gttcttacaa
     7681 gcaagacgag tcgccgcgct accacgcgcg tgatgtggcg gtgttcaggg cgaagcgcgc
     7741 cggcgccaca gtcgtgcttg gttcggcgac accttctctt gagacataca gcaaggctga
     7801 atcgggcgag tacgagctga tcgagcttcc caggcgaatc gacgaccgac ccatgcccga
     7861 cgtggctgta gtggacatgc ggtccgaact gaaggcgggg aatcgtacta tcttcagccg
     7921 tctcctcagg gcccgtattg agcagacact cgaacgcggg cagcaggcga tcctcttcat
     7981 gaacagacgc gggttttcca catttgtctt atgcagagaa tgcggtcatg tgatgacatg
     8041 cccacactgc gatgtctcgc ttacacttca cggctcgggg tcgcgccggt ctgagctggt
     8101 atgccattac tgtaaccata ccgaacgcgc ccctgatgtc tgtccatctt gcggcggcat
     8161 acatatcagg tacttcggcg ccggaaccga gcggatcgag gacgaggtca ggaaatcttt
     8221 ccccaaggcg cgagttgcgc gaatggatgt cgatactacg cggcgcaagg gggcgcaccg
     8281 ggcgatactc ggcgcgttca gggccgggcg caccgacatt ctcgtgggaa cacagatgat
     8341 tgccaagggt ctggattttc ccaatgtcac gctggtagga gtcgtgtcgg ctgacaccgc
     8401 tctaaacctt ccag
//
LOCUS       DAOTAC010000082         8394 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_7227_length_8394_cov_4.717472, whole genome shotgun sequence.
ACCESSION   DAOTAC010000082 DAOTAC010000000
VERSION     DAOTAC010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 8394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8394
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7227_length_8394_cov_4.717472"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="PLM74_04920"
     CDS             <1..458
                     /locus_tag="PLM74_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HON42461.1"
                     /translation="SVKDLPNKAKVGIPNDPTNAGRALLLLQKAGLIGVDKKAGITAT
                     VFDIVSNPKNIQIIELDAAQLPRSLEDMDLAIINSNFALEAKLVPTKDALFMEDSDSP
                     YANVLAVRNVDKGNPALRELAQALISKELADFIARKYQGSVVPAPSLNF"
     gene            complement(570..1070)
                     /locus_tag="PLM74_04925"
     CDS             complement(570..1070)
                     /locus_tag="PLM74_04925"
                     /inference="COORDINATES: protein motif:HMM:NF025879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HON42462.1"
                     /translation="MRVRVNRLRSHQSRSDSEIQVHQLDETTFIGFALRTSTSAPKDV
                     IPRFWDEFWRTGKAEVLSKALGVSCLEPLYGACADYDSSSGAFTYLVGAMLPQGSSVP
                     DGFDSVTLCPSLYGVVHVPMDVSRIQAAWCEVRVWGSKAGFEVGPDGFEAYPDENTCD
                     VYVQIR"
     gene            complement(1426..2223)
                     /locus_tag="PLM74_04930"
     CDS             complement(1426..2223)
                     /locus_tag="PLM74_04930"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HON42463.1"
                     /translation="MIRTLGELGECLFAGRTAEVYAWSATEVVKLYQPWVARVTAERE
                     RASTQVALDMGISVPEVGDIVTVDGRRGLILERIRGTTMMNQIESDVARAGCFAGQLA
                     EIHVAISSVVADERLPEQSGVLQDKIAHCESLTESARRMMLASLAQMPRADRLCHGDF
                     HPGNVILSGDRLFVIDWIDASRGNPVADLARSSILFMGHIAVTPCSSAVRCTMELFHS
                     TYLDICMQKTGVDRDEYAMWLPIVAAARLSEGIPEFRDWLMEQARVP"
     gene            2365..4728
                     /locus_tag="PLM74_04935"
     CDS             2365..4728
                     /locus_tag="PLM74_04935"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HON42464.1"
                     /translation="MAQAITGAQDHLHPYLAAAMERAECIRMIVAFVMESGTRLIAPD
                     LKRAAARGVPIRLLTGQYLQVTEPSALYHLLAELGAAIDIRFYDGDIQAFHPKAYILD
                     YQGEADVFVGSSNLSRSALSTGVEWNYQFRRSEHPDEYARFSDTFEALFHRHSQRVTE
                     ELLKSYALKWKKPALVRSADEEVHLAPDPTPRPRGAQIEALYYLRQAREEGVSKGLVV
                     AATGVGKTHLAAFDSAEFRRVLFLAHRQEIVDQAHEVFRSIRPESRFGLYTGERKDED
                     AGVYFATVQTLSRDEHLQVFPPDYFDYVVVDEFHHAAADSYRKVIDHFRPRFLLGMTA
                     TPFRMDNKDIFALCEDNVIYEISLKQSIERDLLSPFQYYAVYDPTNYDGVRMANGLYV
                     IEDLERQLSRKERADLILRHYTRLAGERTLAFCASIKHAEHMAAFFSEHGVPAAAIHS
                     EQACTDHALDRATAIDELECGRLNVVFAVDMFNEGVDIPSLDTVMFLRPTESFVVFLQ
                     QLGRGLRKYPGKSHCTVIDFIGNYKRAHYLPRLLAGENPWADRPTAPRRAQEYEFPEG
                     CSVSFDFRVIELFDELAAGDPLPLRMLDTYWRIKHSLGRRPTRLDIYQGSDIPIREYL
                     RRGWLRFLESAGDLDAEEATWLGQPAEEFLREVERTHLTKAYKLPTIAAFLRDDGSMS
                     GKVGLDEIGRSMMRFYADHPLHQKDLNNESNRGWQQWSSDDFTRLARRNPVTFLSRGR
                     FFKYDEVDRVMHLSPKIHTHLTPGFASHVQDILEFRRVDYFRRRFKD"
     gene            4748..5134
                     /locus_tag="PLM74_04940"
     CDS             4748..5134
                     /locus_tag="PLM74_04940"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HON42465.1"
                     /translation="MHECVFCSIPDDEVIAQNDLALAFFDRYPVNKGHVLIIPRRHVA
                     TYFDATPDETNAIASLLAEVKAQLDAAFAPDGYNIGVNVGAAAGQTIFHLHVHLIPRY
                     VGDVPDPRGGVRRIKKNLVPYAGEGE"
     gene            5137..5451
                     /locus_tag="PLM74_04945"
     CDS             5137..5451
                     /locus_tag="PLM74_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004214789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HON42466.1"
                     /translation="MGLDYDKLVRDRIPEIIESSGKRAVTRVAEDAEYLSYLHRKLFE
                     EVDEYTASGDVQELADILEVISALGRRAGVPLSRLIEIADDKRRARGGFDSRIVLIRV
                     ED"
     gene            complement(5933..7573)
                     /locus_tag="PLM74_04950"
     CDS             complement(5933..7573)
                     /locus_tag="PLM74_04950"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HON42467.1"
                     /translation="MSAQAAAELVQRSAVDDGITGVPASVVDASGDDAAAEVDPWLLS
                     PSRQRTDKPIPGPLPKSVRVVQANMVYIDKQGLPPAMITRLRRLAAFQNSEFYSAQRM
                     RLSTFGQPRVICCAEDFPEYAALPRGCLDRAIDLLSEHGISPIVQDHRYGGVPEEFVF
                     LGQLNPRQEDAVRVLSSFDDGVFSAPTGFGKTVIAAWMIAHRKVNTLVLVHRRQLLDQ
                     WRAQLSAFLGLGSDSIGQIGGGKVAPTGALDVGMIQSLNRGGEVDDIVAAYGHVVVDE
                     CHHISAVSFEQVLRSVKAKYILGLTATPIRKDGRHPIIMMQCGPLRARLSPRTASRGQ
                     FEHVVIPRATEFRLPDGAADATIQQIYGMLAGDPDRNAMIIADVIQAVRAGRSPLVLT
                     ERTAHLKELVEGLKGYVANIIVLRGGRTDRQRRQLAATLAAVPDCKERVIVATGRYAG
                     EGFDDARLDTLFLALPISWKGTLQQYAGRLHRSYEGKRVVQVYDYVDAYVPILSRMYE
                     RRLRGYKAMGYVVTDADPVRPADGSLSGLFQKMRVNGN"
BASE COUNT         1865 a         2606 c         2323 g         1600 t
ORIGIN      
        1 gcagtgtaaa agacctgcct aacaaggcca aggtgggcat ccccaacgac cccaccaacg
       61 caggcagggc gctgcttcta cttcagaagg ccggactaat aggcgtggat aagaaggccg
      121 ggatcacggc cacggtgttc gacatcgtaa gcaaccctaa gaacatacag ataatcgagc
      181 tcgacgcagc acaactccct cggtcgctgg aggacatgga tctggcgatt atcaactcca
      241 acttcgcctt ggaagccaag ctggtgccta ccaaggatgc actgttcatg gaggacagcg
      301 attcgcccta tgccaacgtg cttgccgtca ggaatgtcga caagggcaac cctgcgctaa
      361 gagagttggc acaagcgcta atatcgaaag agctggctga cttcatagcc aggaagtatc
      421 aaggatcagt ggttcctgcg cctagcctga atttctaggc ccgcgagttc cggtgatcgc
      481 gagtcccgcc cctgcggaag gctgggccaa ctggcccagc ctcctcatct tccggctatg
      541 ccggcccgca actcactcgg cctggccgat cagcgtatct gcacgtacac atcgcaggtg
      601 ttctcgtcag gatacgcttc gaaaccatct ggacccactt cgaagcctgc tttgctgccc
      661 cacacgcgta cctcgcacca ggcagcctgg atccgggaca cgtccatggg aacgtgaact
      721 actccataca gcgacggaca gagcgtcacg ctatcgaaac catcagggac tgaactgccc
      781 tgcggcagca tggctcccac cagatacgtg aaggcgcctg acgacgaatc gtagtctgcg
      841 cacgcgccgt agaggggctc gaggcaggac acgccgagag ctttgctcaa gacctccgct
      901 tttccggtcc gccagaactc gtcccagaag cgaggtatca catccttcgg cgccgatgtt
      961 gacgtgcgaa gagcaaagcc tatgaatgtg gtctcgtcca gctgatggac ttggatctcg
     1021 ctatctgacc gactctgatg gcttcgcagc ctgttcaccc tgacgcgcat gtcatcgatg
     1081 tgggcctcct gtgtgattcg agggtccttt agctccagca ttcgcttgca cgggaccgca
     1141 tcgcattctc cacaggttcc aaacccctta ttggaagcgc attcgaaaag ttcacacact
     1201 tcgataccaa cgtccttcgc ccatgaaggg cgccccgcaa cctcaataca tccgccgcag
     1261 cccttcccga aggcctcaca ctcatcacaa agcagaccgc atgcactcaa caagaccaat
     1321 gcctccctcc gtcgcttgtt cgcccgtcgc acacgccact gagagtatgt cgtatcagtt
     1381 gactccttga accaatgagt tcgctaccag tcagggatga acacgttagg gaacccgcgc
     1441 ctgctccatc agccaatccc gaaactcggg tatgccctca ctcaggcgtg cggccgcgac
     1501 gattgggagc cacatcgcat actcgtcccg gtccacgcca gtcttttgca tgcagatatc
     1561 caggtatgtg ctgtggaata gctccatggt gcaacgtaca gctgacgagc agggcgtaac
     1621 cgcgatgtgc cccatgaaca ggatcgagct tcgagcaaga tccgccacag ggttgccgcg
     1681 agaggcgtca atccaatcaa tgacgaaaag cctatcgccc gacaagatga cgttcccagg
     1741 gtgaaagtcg ccgtggcaca gacggtctgc gcgcggcatc tgggcaagcg atgccagcat
     1801 cattcggcgc gccgactcag tgagcgactc gcagtgcgcg atcttgtcct gcaggactcc
     1861 gctctgttca ggaagtcgtt catcggcaac gaccgagctg atcgcgacgt gtatctcagc
     1921 cagttgacct gcaaaacacc cggcgcgagc aacatcggac tcgatctggt tcatcatcgt
     1981 ggtgcccctg atgcgctcga gaattagccc gcgtcgccca tccacagtga cgatgtcgcc
     2041 aacttcagga acagagatcc ccatgtccag agccacctgc gtactggccc tctcccgctc
     2101 ggccgtcact ctggctaccc agggctggta cagctttaca acttcagtgg cgctccaggc
     2161 atagacctcc gctgtccgcc cggcgaagag gcattcgcca agctccccca gtgttcggat
     2221 catgaagcgg cctccaaaca actcagctgg ctccactata cgccaccagt acaggcagtg
     2281 tcaaagatca ggaactcgaa ggattcctgg caccagtgga gtaactgtta cctgagcgca
     2341 tagactgccc gcgaggtgac agcggtggcc caggccatca ccggtgcaca agatcatctc
     2401 catccttatt tagctgccgc catggagcgt gctgagtgca taaggatgat cgtcgcgttt
     2461 gtcatggagt caggaaccag gctcatcgca cccgacctga aacgggctgc ggcgcgcggg
     2521 gttcccatta ggctactcac agggcagtac ctgcaagtca ccgaaccgtc tgctctgtac
     2581 cacctcctcg ctgagctagg cgccgccata gatattcgtt tctatgacgg tgacatccag
     2641 gctttccacc ccaaagccta catccttgac taccaaggcg aagccgacgt cttcgtgggc
     2701 tcgtccaacc tgtcacgctc cgccctctcc accggggtcg aatggaacta tcagttccgg
     2761 aggagcgaac accccgacga gtacgcgagg ttcagcgaca cattcgaagc gctgttccat
     2821 cgccactccc agcgtgtgac ggaggagttg ctgaagtcgt atgcattgaa atggaagaag
     2881 ccggcgctgg tacggtccgc ggacgaagaa gtccatctgg ctcccgatcc gacaccacgg
     2941 ccccgcggcg cacagattga agccctctac tacctgcggc aagctcgtga ggaaggcgtc
     3001 agcaaaggat tggtagttgc cgccactggt gtaggcaaaa ctcacctggc cgccttcgac
     3061 tcggcagagt ttaggcgcgt tctcttcctg gcccacaggc aagagatcgt agatcaagcc
     3121 catgaggtat tcaggtccat tcgcccggaa tccagattcg ggctgtatac aggtgagcgc
     3181 aaggatgaag acgccggagt gtacttcgcc accgtgcaaa ccctgagtcg cgatgagcat
     3241 ttgcaggtct ttccccctga ctacttcgac tatgtggtgg tagacgagtt tcaccatgct
     3301 gcagccgaca gctaccgcaa ggtaatcgac cacttccggc cccgctttct cctgggcatg
     3361 accgcaacac cattcagaat ggacaacaaa gacatcttcg ctctatgcga ggacaacgtc
     3421 atatacgaga tctctctcaa gcagtcaata gagcgcgact tgttgtcacc gtttcagtac
     3481 tatgcagtgt acgacccaac caactacgac ggggtacgaa tggcaaacgg gttgtacgtc
     3541 atagaagacc tggagcggca attgtctcgc aaagagcgcg cggatctcat actccggcac
     3601 tacacaaggc tggcaggcga acgtaccctg gcattctgcg cctccattaa gcacgcagag
     3661 cacatggccg ccttcttctc cgagcacggc gtgccggcag cagccatcca cagcgagcaa
     3721 gcctgcacag accacgcgct cgacagggct accgccatcg atgagctcga gtgcggtcgg
     3781 ctcaatgtgg tcttcgcagt agacatgttc aatgagggcg tcgacatacc atcgctcgac
     3841 actgtgatgt tcctccggcc cacggagtcc ttcgtagtct tcctgcagca attagggcgc
     3901 ggcctgcgca agtatcctgg caagagtcat tgcactgtga tcgatttcat cgggaactac
     3961 aagagagcgc actacctacc ccgtctcctc gccggagaga acccgtgggc ggatcgaccg
     4021 accgcaccca ggcgggcgca ggagtatgag tttcccgaag gatgctctgt cagtttcgat
     4081 ttcagggtaa tcgagctctt cgacgagctg gccgctggtg accccctgcc tctcaggatg
     4141 ctagatacct actggcggat caagcactcg ctcggtaggc gccccactcg actagacatc
     4201 tatcagggta gcgacatacc tatcagagag tatctaagac gtggatggct gagattcctc
     4261 gaatctgcgg gcgatctcga cgctgaggaa gcgacatggc tgggccagcc ggcagaggaa
     4321 ttcctgaggg aggtggaacg tacgcacctg actaaagcct acaagctgcc cacaatagcc
     4381 gcgttcctaa gagacgacgg cagcatgtca ggcaaggtcg gtctagatga gataggccgg
     4441 agcatgatgc gcttctacgc cgatcatcca ctgcaccaaa aagacctgaa caacgagagc
     4501 aaccgaggtt ggcagcagtg gagttcagat gacttcacca ggctggcgcg gcggaatccc
     4561 gtcacctttc tgtctcgggg acgattcttc aagtatgacg aagtcgaccg tgtgatgcac
     4621 ctcagcccaa agattcacac acacctcacg cccggattcg cgtcgcacgt gcaggacatc
     4681 cttgagttca gacgagtaga ctacttccgt cgcaggttca aagactgaca gcgaaggact
     4741 gatactattg cacgaatgcg tcttctgcag catccccgac gatgaagtaa ttgcccagaa
     4801 cgacctcgca ctggctttct tcgacagata cccggtgaac aagggccacg tgctgatcat
     4861 acccaggcgc cacgtggcca cctacttcga tgccactcca gatgagacga acgccatagc
     4921 cagtctgctg gccgaggtta aggctcagct agacgccgcg ttcgctcccg acggctacaa
     4981 catcggagtg aacgtgggcg ccgccgccgg ccagaccatc ttccaccttc acgtacacct
     5041 gatcccgagg tacgtaggcg acgtgcctga cccgcgcggc ggcgtgcgga ggatcaagaa
     5101 aaacctagtg ccgtacgctg gggagggcga gtagacatgg gactagatta cgacaagctg
     5161 gtcagagaca ggatcccgga gatcatagag tcatcgggaa aaagggccgt gacgcgcgtt
     5221 gccgaggatg ccgagtatct gagttacctt cacaggaagc tgttcgagga agtagacgaa
     5281 tacaccgcca gcggggatgt tcaagaactg gccgacatac tcgaagtcat ctcggcgctc
     5341 gggcgccggg cgggcgtgcc cttgagcagg cttatcgaaa tcgctgacga caaacggcga
     5401 gccagaggag gattcgatag ccgaatcgtg ctgatacgcg tggaagacta gttgatagcg
     5461 gagacccgta ggcagatgca ggaccaatgc tatgcctcag gccctactcc ggcctcgctg
     5521 aacacccgca ggtatgggag atatgcgaca ggcgcttagg gaaacccgcg catgccagcg
     5581 aattagcgga cctgcaagaa atgtgactgg ttttttgacg aacctgctca ggatgtgtca
     5641 ggtttgaaca ccgaggaagg gaaggggtgg tcggatgtgg gttttcatag tagactggca
     5701 ggcatttggg acctttccgg acaccctcga gagcgtcacg cataccgcca tattatgtaa
     5761 tccgcccctg cctgcggatt tgctgcaggc gtgactccag aaaggggaac agggcgcccc
     5821 cagatccccg taggggtaca ttaccggcaa acgcggcacc cagaaacatc ctgcagcgca
     5881 gtacaccgaa cctaattagt gcaggcctta actagccggc cgaagtcaga cgtcaattac
     5941 cgtttacccg cattttctga aacaatccac tcagtgagcc gtccgccggc ctgacggggt
     6001 cagcgtccgt caccacatac cccatcgcct tgtatcccct caggcgtctt tcatacattc
     6061 gcgacaggat gggcacatag gcgtctacgt agtcatacac ctgcactacg cgctttcctt
     6121 cgtatgagcg atgaagccgc ccggcgtact gctgaagtgt gcctttccat gatatcggaa
     6181 gggcgaggaa taacgtgtcg aggcgagcgt catcaaaccc ctcccctgca tatcgccctg
     6241 tggccacgat cacgcgctct ttgcagtcag gcaccgcagc gagcgtcgca gcaagctgcc
     6301 gcctctgcct atcagtgcgc ccgccgcgca gcacgattat gtttgcaaca taccccttga
     6361 gtccttcgac cagctccttc agatgggcag tgcgctccgt cagcaccagg ggtgatcggc
     6421 cggcacggac agcctgtatg acatcagcga taatcatggc gttgcggtcg ggatcgccgg
     6481 caagcatgcc atagatctgc tgtatcgtag catcggccgc accgtcgggt aaccggaact
     6541 cggtcgcccg gggtatcaca acatgttcga actgaccgcg cgatgccgtt cgcggactga
     6601 ggcgcgctct cagcggtccg cattgcatca ttatgatcgg atgacgtccg tccttccgaa
     6661 tcggagtagc agtcaggccc agtatgtact tggccttcac gctcctcagc acctgttcga
     6721 agctgacagc cgagatgtga tggcactcat ccacaaccac gtgaccatat gccgccacta
     6781 tgtcgtccac ctcgccgccg cggttaaggc tctgtatcat gcctacatcg agcgcgcccg
     6841 tgggggcgac cttacccccg cctatctgtc ctattgagtc agacccaagt cccaggaatg
     6901 cagacagctg cgcgcgccac tgatccaaga gctgcctccg gtggaccaag accagggtgt
     6961 taaccttgcg atgcgcgatc atccaggccg cgatgaccgt cttgccaaag cctgtcggag
     7021 cgctgaagac gccgtcgtcg aacgatgaaa gcacccggac cgcgtcttcc tggcgaggat
     7081 tgagctgccc gaggaacacg aattcctcag ggactccgcc atacctgtga tcctggacta
     7141 tcggcgagat tccgtgctcc gatagcaagt cgatagcccg atccagacaa ccgcgaggaa
     7201 gcgccgcgta ctccgggaag tcctcagcgc agcagatgac acggggctgt ccgaaggtgg
     7261 agagacgcat cctctgagcg ctgtagaatt ccgagttctg aaatgccgcc aggcgccgca
     7321 gacgcgtaat catagccggc ggcaggccct gcttatcgat gtacaccatg ttcgcctgaa
     7381 ccacacgaac actcttgggc agaggccccg ggatgggctt gtccgtcctc tgtcgcgacg
     7441 gcgataagag ccacgggtca acctccgcgg cagcatcgtc accagacgca tcgaccacac
     7501 tcgccggaac acctgtgatg ccgtcgtcga ctgcagatct ctgcactagc tcagcagcgg
     7561 cttgagcgct catgcaagga gttcttgcat ccagagcaag gcttgcgaca gagccgagga
     7621 ttccattcgt tggcgcaggc cggcgagtag ccggatctgc catctctgct atcccaacgt
     7681 ttggcgtaca catcatcccg cccacggaac agactgcgga acaacgcgac cttttccgcg
     7741 accggcgatg aagatgtgac agatgcaacc gagtcaccag ggcgaacagg cgatgcagat
     7801 ggaacggaca ttgcgggcgg agcgggcgag atgggttggg cagacctgga atgcgtgaca
     7861 tggcaagcct ccatgcgctg tcgcagggaa gcgttctcgt tgcgcaggct ctcacactct
     7921 gcagctagtc tcttgatctc ggcctccaat tgctcaatgc tcttcatacg gaaatcacca
     7981 gctgctagac aatcttcctg ggcagtatgt tcaccgccgc cgctgacccg tgctcgccag
     8041 ccggtacgca cggtccatcc agttgccttc actgtacatg acgcacgccg tcactgtcaa
     8101 aacccactcc catccgcgat cagccggccc gccgcaatca cagtcgaacg ccgcggccaa
     8161 cctgtaccct gtgggccagg atttccgcgg cacctgtgtc aaccgactca ggatggccga
     8221 aaaacgtgtc ccgtagggca caggagacga tcgccggcct tcccccggac ccctagaccg
     8281 gacgtggcag tttcggcgca ggcgccctag ctgcggcttc cccggacgca gatttcgcac
     8341 gccgcaatca cagtcgaacg ccgcggccaa cctgtaccct gtgggccagg attt
//
LOCUS       DAOTAC010000083         8373 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_7252_length_8373_cov_4.406227, whole genome shotgun sequence.
ACCESSION   DAOTAC010000083 DAOTAC010000000
VERSION     DAOTAC010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 8373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8373
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7252_length_8373_cov_4.406227"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            189..1631
                     /locus_tag="PLM74_04955"
     CDS             189..1631
                     /locus_tag="PLM74_04955"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HON42468.1"
                     /translation="MSNKRSSIVVVAIGLALLGLVLIAPLPGVSTPSGEEIELDWRGR
                     ASLFALGFAALLWATEALPVAFTGLVAVALLPLLGAMQWDQAVRAGLTSDVIPFLVGI
                     MITSEAIASSGLANRLSRAIAARVGNHPKTAVFILLVTGAALSMCIGNLSAAAIVMPL
                     GLALLEANRAPKGRSNLGKAIMIACAWGPLIGAVATPAGSTSNVLAMGFLSELAGVNI
                     SFVQWMAVALPAALLMIVPAWAILVTLFPPEPDALGACMADARTVADRALTRDEWSVV
                     AGIAAMWGVWLGAGSLEMVTGMRVSPSLGAILGALVTLSLRSGGIKWADVESTVSMGT
                     IITVASGLSLGSAIHSSTAGKWLANMLFSPIMGMPLFPRALLLAASICLLKTMFSSNT
                     ATAAILMPVLISLVAGLPGQDSTAIWQLVAPAAMATSLSTILATSSPTSLLAYRAGYF
                     TTADMAKAGLLFLVPASVSLAAACVLLIRG"
     gene            1767..2414
                     /locus_tag="PLM74_04960"
     CDS             1767..2414
                     /locus_tag="PLM74_04960"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HON42469.1"
                     /translation="MKAALIIAMNFFLGSVPWAVIVGRALRGVDITIYGDHNPGTYNA
                     LRAGGLRLAAPAMILEFAKGFTAGRLAQVMLAASPAAASWAWTAAVVAPVAGHGFTPF
                     LRFRGGKALAVTLGVWTAIAGWPAMLVYAGTLCLQELTWPKAPDALKVSLGMSALGTV
                     AWLMKLPARYGAAWALTFAVMLIKQWQYETVFQCTADRYGTGINDNRPGAKGNCG"
     gene            complement(2430..>3029)
                     /locus_tag="PLM74_04965"
     CDS             complement(2430..>3029)
                     /locus_tag="PLM74_04965"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HON42470.1"
                     /translation="TLPFFCKYVLGLSDAQNTLVLAPIFIVALPMVWVWGRITNRLGP
                     RHASMLAVAVFAIALVPFAFVNSLVTVIITTAFTGVGLAGVLVLVDVLIADVIDEDET
                     HTGARREGAYFGANAFIMRFSVSLEAIAFSVVMGIHGYNPVLEVQPAGVVPGLRFLMT
                     VVPLVSLALAMIALYFYPIDGARRAHITATLSQRRGEAQ"
     assembly_gap    3030..3129
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3130..3563)
                     /locus_tag="PLM74_04970"
     CDS             complement(<3130..3563)
                     /locus_tag="PLM74_04970"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HON42471.1"
                     /translation="MRPRIGSRYGTGSGTFLYCLGSFTAALLGQAFSSWAQLYYVDIL
                     KLPLVLYGTGMTIYGIWNAINDPLAGQWSDRTRTRWGRRIPFILFGTVPFCAVFILVW
                     TVPQSVQASASSLFAYFLGMAFLFDGLFTVVILNWTALFPEM"
     gene            3676..5088
                     /locus_tag="PLM74_04975"
     CDS             3676..5088
                     /locus_tag="PLM74_04975"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HON42472.1"
                     /translation="MVGVELPWQLHVGQVRFLMITSETQLRKATALMLAAVFIVLLAA
                     PRILCFEMGSETHESSAPAELTYSTQERRRICEEVWRKVGLWYSYFDDKGIDWASVKL
                     RYLDLAAGARSDREFFSCITNLLRELHDGHSYVYEYPKPVPGMGKRGTPGVRVVEAEG
                     RPVVAEVTPGSDAEARKVVPGLEIVSVDGQSAEERIRSVIPVITASTTWYARSAAVTA
                     ILNGDLDEAVRVELRRPEGTVFSVSLAREPFERRSEAISAQILSGGIGLLRLPSFSAS
                     NMGLKSGDALVKAFDDALAELKDTRALIIDMRGNGGGDDSVADRCAGRFFTSATAFPG
                     FQMRMVTLGMPWFAPRLARSVSPRGTWQYTQPVVLLIDEFVFSSAEHFVAGMHDSERA
                     TTIGHTTAGSSGNPMQLEVSGFKLQVSRWREYRADGSLIEGHGVPADIVARPSIADLA
                     NGVDRVLAAAVDYLDHLASR"
     gene            complement(5153..6808)
                     /locus_tag="PLM74_04980"
     CDS             complement(5153..6808)
                     /locus_tag="PLM74_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42473.1"
                     /translation="MHDRRLRTPTAVTTAITVAIAVTIAAVAALIPLPASAAQTLPAA
                     DRIHARAQADVTGDGVDDSILLVGHPYGSDSQFCTSHDVVVYDGVTQDRITMVLGGTS
                     GGYPGTIFVGRIDNDNALDVVVTIPTGGSGGITNPFVVSFAGGKARFIGDPQAMARGA
                     KLEANGLDHFRVRITDTERGKHFILGLPGPGSAQDPELDYYDGIYSKSGKLIRPLDID
                     IDDVGAIEAASIPGTGLDELVTYQKVWAVAHVNTIGTVRTVWRWTGRQIAIAAVDVIP
                     PFTPEAYGEYIRSFDKSRPAAAVEKAVAEYKARFGLAPLSIREMAFREFRHFHLTIAA
                     AEGASLEKRAGLADAPKSGEVASAYERLPRLASELNMSDEYKRAGLNAVYDGEGTWTV
                     VPRPGFSVEQFGRLLPQAAADFVGLDDRERNERWQYDAAIVVPLSELGGRVAAWEAYL
                     AKYPDSMFADEARTYFKRGLSALLMGTNNTPHFNYGTKRVRPDVIDALKAYAKDYSGT
                     PSAEAVTRALKAIEKNGNVITDAVRREINRAIEQATKADAGQA"
     gene            7031..7558
                     /locus_tag="PLM74_04985"
     CDS             7031..7558
                     /locus_tag="PLM74_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003869712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HON42474.1"
                     /translation="MLSERLVSELNRQINYELYSAYLYTAMETYFLDRGLTGFANFFK
                     VQTQEELAHARIFFEYVYRKNGHVALEAIDKPEANFASILDVFEKALAHEQFVTSRIY
                     KLVDIAIEEKEHATNSFLKWFVDEQVEEEESFQGLVDRLNLMGDKPSNLFMIDADLAQ
                     RVFTLPAPLAPSAQA"
BASE COUNT         1616 a         2359 c         2554 g         1744 t
ORIGIN      
        1 ttcaactaca tgtcccagca cgggatgtac aacccgaagt aaccgcactt tacactgtac
       61 gcacggaggc tccgtctgct aagtcaggac ggagcttcct gtcgtggatt ccccttcctc
      121 aggttgaagc ctcgaaggga tggtggtatg atgcagccag cgagagtcgc tagtgaggat
      181 gtcgaagtgt gtcaaacaag agaagtagca ttgtcgttgt cgccattgga cttgctttgc
      241 tcgggctagt tctgattgcg ccgcttccgg gggtatctac accgtccgga gaggagatcg
      301 agctcgattg gcgaggccgg gcctcgctat ttgctctagg ttttgcggcg ttgctctggg
      361 cgactgaagc tctcccggtt gctttcaccg gactagtggc agtggcgctt ctgccgctgc
      421 ttggggccat gcaatgggac caggcagtcc gggctggcct gacaagcgat gttatcccgt
      481 tccttgtggg gatcatgatc acatcggaag ctatcgcctc ctcgggattg gccaacaggc
      541 tctcgagggc catcgccgcc cgggtaggca accatccgaa gactgcagtg ttcatactgc
      601 ttgtaactgg ggccgctctt tccatgtgca ttggcaacct gtctgcagcg gccatcgtca
      661 tgcctctcgg gcttgccctg ctcgaggcca accgggcgcc gaagggcagg tccaatctgg
      721 gcaaagccat catgatagca tgcgcctggg gcccactaat aggcgctgtg gctacgccgg
      781 ccggttctac ctctaacgtg ctggccatgg ggtttctcag cgaactggca ggtgtgaata
      841 tcagtttcgt ccaatggatg gccgtggcac ttccggcggc actgttaatg atcgtgcctg
      901 catgggctat tctggttacc ctgtttcctc ccgaacctga tgctttgggc gcctgcatgg
      961 cagacgcgcg tacggttgcc gatcgggctc tgacgaggga tgaatggtcg gtggtcgccg
     1021 gaatcgcggc gatgtggggg gtatggcttg gggccggcag cctggagatg gtgacaggca
     1081 tgcgggtgtc gccgtcgctg ggcgctatct tgggtgcatt ggtgacgctt tcgttgcgta
     1141 gcggcgggat aaaatgggcg gatgtcgagt ccaccgtcag catgggcact atcatcactg
     1201 ttgcgtcagg cctgtcgctg ggttcggcca ttcacagttc gacagccggg aagtggcttg
     1261 ccaacatgtt gttctctcct atcatgggga tgcctctgtt cccccgtgcg ctcctactgg
     1321 cggcaagtat ctgcctgctc aagaccatgt tttccagcaa taccgccacc gctgcgatcc
     1381 tgatgcctgt cttgatctcg ctggtggcag gcctacctgg tcaggactcg actgcgatct
     1441 ggcagctggt cgctcctgca gctatggcca catctctttc gactatcctt gcgacatcct
     1501 ctcccaccag cctgctggcg tacagggccg gctatttcac caccgccgac atggccaaag
     1561 caggacttct cttcctcgtt ccagccagtg tgagcctggc ggcagcttgc gtgctgctca
     1621 ttcgcggata gcggggcgag tgaggcgcgt aggcccgagc acactgacga caccggaacc
     1681 tcgcccgccc gcagggattg ccgaatggct atcgaattcc tctggtcaga caacggacaa
     1741 tcctgaagga agcgaggtct gcccaattga aggcggctct gatcatagcc atgaacttct
     1801 tcctcggttc ggtgccatgg gccgttatag ttggacgagc tctcagagga gtagacatca
     1861 ctatctacgg ggaccacaat cctggcacgt acaatgcgct gcgtgccgga ggcctcaggc
     1921 tggctgcgcc cgccatgata ctggagtttg cgaagggctt caccgcaggg cggctggcgc
     1981 aggtcatgct ggcagctagt ccggcagcgg cgtcctgggc gtggacggct gccgtggtgg
     2041 cacccgtcgc cgggcatggt ttcactccat tcctacgctt ccgcggcggc aaggcgctgg
     2101 cagtgacgct tggagtctgg accgccatcg ccgggtggcc cgcaatgctg gtatatgcgg
     2161 ggactctatg cttgcaggag ctcacatggc ccaaggcgcc ggatgcgctc aaggtctcgc
     2221 tgggcatgtc ggccctgggg actgtggcat ggctcatgaa gctgcccgca cggtatggcg
     2281 cggcctgggc cctgaccttc gcagtcatgc tcatcaagca gtggcagtac gaaacggtat
     2341 ttcagtgcac ggcagacagg tacggcactg gcataaatga caaccggccg ggcgccaagg
     2401 gcaattgcgg ttgagccggg gcatttgcgc tattgggctt ctcctctgcg ctgactgaga
     2461 gtcgcagtta tgtgtgcccg cctcgcacca tcgatggggt agaagtaaag tgcaatcatg
     2521 gcgagcgcca gcgaaactaa ggggactact gtcatcagga agcgcagtcc tggcacaacg
     2581 ccagccggct gaacctcaag cacggggttg tacccgtgta tgcccatcac tacgctgaag
     2641 gcgatggcct ccagcgagac gctgaacctc atgatgaacg cattggcgcc gaagtacgct
     2701 ccttcgcgcc gggcgccggt gtgggtctcg tcttcatcga tgacgtcggc aatgagcaca
     2761 tccaccagca ccagaactcc cgccaagccc acaccggtga atgccgtagt gattatcaca
     2821 gtcacaaggc tgtttacaaa cgcgaatggc acaagggcta tagcgaatac tgccactgcg
     2881 agcatgcttg cgtggcgcgg acccagacgg tttgtgatgc gcccccaaac ccacaccatc
     2941 ggcagcgcta cgatgaaaat gggggccagc accagcgtgt tctgggcgtc gctcaggccg
     3001 agcacgtact tgcagaagaa aggcagtgtn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnt acatctccgg gaaaagggca gtccagttca ggatgacgac cgtgaatagc
     3181 ccgtcgaata ggaaagccat gccgaggaag tacgcgaaca ggctgctcgc gctggcttgc
     3241 actgactggg gcactgtcca gacgaggatg aatacggcgc agaacgggac cgtgccaaag
     3301 aggataaacg gaatccgcct gccccagcgg gtgcgagtgc gatcagacca ctgccccgca
     3361 agggggtcgt tgatcgcatt ccagatgcca tagatcgtca tgcccgttcc atacagcacg
     3421 agcggcagct tgaggatatc cacgtagtac agctgtgccc aggagctgaa cgcctgcccc
     3481 agcagtgccg cggtgaagga acccaggcag tacaagaacg tgcctgaccc agtgccgtac
     3541 ctgctgccta ttcgcggtct catgccatcg cctccccaag aacgctaaga acgctcgctt
     3601 cgaagcaatt tcgacaacca agctttctca ccttctccgc gggttcggat caagttgact
     3661 gcgcggagga tatctatggt aggtgtcgaa ctaccgtggc aactccatgt cgggcaggtg
     3721 agattcctaa tgataaccag tgaaacacag ctgcggaagg ctactgcctt gatgcttgcg
     3781 gcagtgttca tcgttctgtt ggcggcgccg cgtattctct gtttcgaaat gggttccgaa
     3841 acacatgaga gcagcgctcc tgctgagctg acgtattcga cccaggagcg ccggcggatc
     3901 tgtgaggagg tctggcgcaa ggtaggccta tggtacagct acttcgacga caagggcatc
     3961 gactgggcgt cggtgaaact tcgttatctt gatttagctg ccggggcaag gagcgaccgc
     4021 gagttcttct cctgcataac taatcttctg cgagaacttc acgacggcca ttcatacgtc
     4081 tatgagtacc cgaaaccggt ccccggcatg ggcaagagag ggacacctgg cgtccgcgtg
     4141 gtggaggcag agggccggcc cgtcgtggcg gaggttacgc caggctccga cgccgaggcc
     4201 aggaaagtcg taccaggcct tgagattgtg tcagtagatg ggcaatctgc cgaggagcgg
     4261 attcgctcgg tgattcctgt gatcactgct tcgacgacat ggtatgcgcg gagtgccgcg
     4321 gtgaccgcga tcctaaacgg cgacctggat gaagcggtac gggttgaact ccgccgcccg
     4381 gagggtacgg tgtttagcgt atcgctggca cgcgagcctt tcgaacgtcg atcggaggcg
     4441 atcagcgccc agatactgag cggaggcatc gggctgctcc gactcccgtc tttctcggct
     4501 tccaacatgg ggttgaagtc tggagacgcc ctggttaaag cgtttgacga cgcactggcc
     4561 gagcttaagg atacacgggc gctaataatc gacatgagag gcaacggtgg aggcgacgat
     4621 agcgtagcgg accgttgcgc aggacgattc ttcacttctg ccacggcgtt tcccggtttc
     4681 cagatgcgca tggtgaccct gggaatgccg tggttcgcac cgcgcctggc gcggagtgtc
     4741 agcccgcgag gaacgtggca gtacacgcag ccagtggttc ttctcatcga cgagttcgtc
     4801 ttcagcagcg cggagcattt cgtcgcgggc atgcacgact cggagagagc gactactatc
     4861 gggcatacca cagcgggttc cagcggcaac cccatgcagc ttgaggtgag cgggttcaaa
     4921 ctccaggtat cgaggtggcg ggagtaccgg gccgacggtt cgctgatcga agggcacgga
     4981 gttccggcgg atatcgtcgc ccgccccagt atcgccgacc tcgcgaatgg agtcgaccga
     5041 gtactcgcgg ctgccgtaga ctacctcgac cacctcgcgt cgcggtgagg caaaggtgag
     5101 gcaaaggtga ggcaagggtg aggcagggga aggatcccct gcctccgtct gactatgcct
     5161 ggcctgcgtc cgcctttgta gcctgttcaa tggctcggtt gatctcgcgt cgaactgcgt
     5221 cagtaatgac gttgccattc ttctcgattg ccttgagtgc gcgggtaacc gcctcggctg
     5281 aaggtgtgcc ggagtagtct ttggcgtacg ccttcagtgc gtcgattacg tcgggtcgca
     5341 cgcgcttggt accgtagttg aaatgaggcg tgttgttggt tcccatgaga agagccgaca
     5401 acccccgctt gaagtaggtt ctggcctcat ccgcgaacat cgaatcgggg tacttcgcaa
     5461 gatatgcttc ccatgctgcg acgcgaccgc ccagttctga cagcggcacc acaatggctg
     5521 catcatactg ccagcgctcg ttgcgctcgc gatcatccag gccgacgaag tcggcagcgg
     5581 cctgaggcag gagccgaccg aactgctcaa cactgaatcc tggacgaggc acgactgtcc
     5641 acgtgccttc accgtcgtag acggcgttga gcccagccct cttgtactcg tccgacatgt
     5701 tgagttcaga tgcgagcctt ggaagacgct catacgctga ggctacctcg ccgctctttg
     5761 gggcgtctgc gagacctgcc cgcttttcca gcgatgcgcc ctcggctgcc gcgattgtca
     5821 ggtggaagtg cctgaactcg cggaaagcca tctcgcgtat gctcagcgga gccagcccaa
     5881 accgggcttt gtactcagct acggccttct ctacggccgc ggcaggacgc gacttgtcga
     5941 acgacctgat gtactctccg tacgcttcag gagtgaaggg cgggatcaca tcgactgcgg
     6001 ctatcgcaat ctggcgacca gtccatcgcc acacggtcct gacggtcccg atggtgttga
     6061 cgtgagctac agcccacacc ttctggtatg tcaccagttc gtccaggccc gtaccgggaa
     6121 tggacgccgc ctcaatagcc ccgacatcgt cgatatcgat gtcgagaggt cggatgagct
     6181 tgcccgactt tgagtatatg ccgtcgtagt agtctagttc ggggtcttgg gctgagcccg
     6241 gtccaggaag ccccagaatg aagtgcttcc ctcgctcggt gtcggtgatc ctgaccctga
     6301 aatggtcgag gccgttcgcc tcgagctttg cgccacgcgc catcgcctgt ggatcgccaa
     6361 tgaagcgggc tttgccgcct gcgaatgata cgacgaacgg gttagtgatc ccgccggacc
     6421 cgcctgtggg gatggtgacc accacatcga gggcattgtc gttgtcgatg cgcccgacga
     6481 agatagtgcc cgggtatccg ccgcttgtgc caccgagaac catcgtgatc cgatcttgtg
     6541 tgacgccgtc gtatacgacc acatcgtgcg acgtgcagaa ttggctgtcg ctgccatatg
     6601 gatgtcccac gagcagaatg gagtcatcta cgccgtcgcc cgtgacgtct gcctgagcgc
     6661 gtgcgtggat gcggtcggcc gcaggtagcg tctgagccgc agacgccggc agcggaatga
     6721 gtgctgcaac tgccgcaatt gttacggcga ttgcgactgt gatcgcggtc gtgaccgcgg
     6781 tcggcgttcg cagtcgccta tcgtgcatag gcattgaaca cacctctgtt tggaacggat
     6841 tttcgggcat gagctgccca taggattatt gtatcaggtg tctgaccagt ttcgggcagg
     6901 tagtatgtgg agacggctcc catacgaatg ggtaccgaaa catggcgaag gggaacccgt
     6961 tcgggcagga atccgatgct gagcagagta tgatatcgag catactactg gaacgaaagg
     7021 aggcgttctg atgctgagcg aaagactggt atccgaactg aaccgtcaga tcaactacga
     7081 actgtactcg gcttacctgt acaccgccat ggagacctat ttcctcgaca ggggtctcac
     7141 cggcttcgcg aacttcttca aggtgcaaac ccaggaagag ctggcacatg cccgcatatt
     7201 cttcgagtac gtctatcgca agaacggcca cgtcgccctg gaggcgatcg ataagcctga
     7261 ggcgaacttc gcgagcattc tggatgtgtt cgagaaggcg ctcgcgcacg agcagttcgt
     7321 gacgagcagg atctacaagt tggtcgatat tgcaatagag gagaaggaac acgcgaccaa
     7381 cagtttcctg aagtggtttg tggacgagca ggtcgaagag gaggagtcgt tccagggtct
     7441 agtggacagg ctgaacctca tgggcgacaa gcccagcaac ctgtttatga tcgatgcgga
     7501 tctagcacag agagtgttca ctctgccggc gcctcttgcg ccgtccgcac aggcatagca
     7561 ggcctggcgc atacccgtat gcgaggacct gctcctgcgc cagggcgccc ctcgtacatg
     7621 ctgttggggg gcgccttctg tgttgtttgt cggatcgaac gggtgcgtgt agggattgca
     7681 ggtaacgaca gcgagcccat taccggcttt ggatccgctg tctccgatgc ccgagttgca
     7741 gcctgtcggc tgcccgacaa gttgccttcg gtccgcgtct ttgtcctctt atctgccagc
     7801 ggcatcctat tgcattagcg ttttcatctg cacgtgtctc cgcccactcc ctattcggca
     7861 ccttctcttg gtcggcgccg cagccgtgga gcccagcggg tcggcggaga ggagtgaatg
     7921 ccgagccctg agagtcacaa tcgctacacg cacccgggcc aagcttgcgt atcctgctac
     7981 atattacttc cggaggcagg agctagttac catacagcag aattcccaca tcagtgagac
     8041 caaagtttcc attcaggcca tcaaagacat gcagattgcc gcctagagcg tgaaagtcgt
     8101 cttgtactgt tacccttcgc aggagaaaag ccatctgagt cgaattgatc atgccaagtt
     8161 tacacgtggg tagttgtacc tatcgatgta cggtccggcg tgtcgggatc cgagaaggga
     8221 aggatgccat gcaacattga gcgtagcttg tcatctgtca gtgagctgaa ccattcccgg
     8281 cggactgccg cggctcagct gcgtgttgcg gccatgactc acttgattgc atgggctgag
     8341 atgaacgaat accgatggaa gtggggtctg ata
//
LOCUS       DAOTAC010000084         8367 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_7261_length_8367_cov_4.410611, whole genome shotgun sequence.
ACCESSION   DAOTAC010000084 DAOTAC010000000
VERSION     DAOTAC010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 8367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8367
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7261_length_8367_cov_4.410611"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..1191)
                     /locus_tag="PLM74_04990"
     CDS             complement(85..1191)
                     /locus_tag="PLM74_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON42475.1"
                     /translation="MASETGMLLDVCDLHTLYPTARGDVRAVRDVSLSVAPREVLGIV
                     GESGCGKSALMLSIMRLLPRPGRIARGSVRLGGVDLLGLSPGEMREVRGKEIAMVFQD
                     PMTTLNPAYRVGEQISESLRVHAMMPSGAGRRGLGRACLRSRLWQARRQREEERDYVI
                     ELMRRVGIPSPDHRHLEYPHQFSGGMQQRIMIAIALACKPRLLLADEPTTALDVTIQA
                     QVLELMEDINCEMGTAIIIVTHDLALAGDFCDNIAVMYAGQIVEWGPADAIMEDPLHP
                     YTRGLLLSMPRLGSRQPLRAIPGEVPDLTSLGSGCAFAPRCRHRTDRCEAEEPPVQAV
                     SGRMLRCHAPQSGMPGTAQFMRQIGRQIGGCMGG"
     gene            complement(1200..>1955)
                     /locus_tag="PLM74_04995"
     CDS             complement(1200..>1955)
                     /locus_tag="PLM74_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HON42476.1"
                     /translation="VAAPVVAPYPPGAVNLAVRLAPPAIRTGNFLGTDQLGRDILSRI
                     MHGARVSMLVGLAATSISAVLGVGLGVVAGYYGGVLDRALARFTELLLAFPYLIFVIG
                     AMSVMGPGFGNLVWALSFKGWVEFYRLSRALVMSEKNREYVEAARVLGRSDGAICVQE
                     ILPNIAGSILVLGTLRMGNMIVMESSLSFLGLGVPPRMPAWGSMINDGRRYMLTSPWV
                     ATFPGLALALLVLGMNLLGEGLRDVLDPKIMEG"
     assembly_gap    1956..1965
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1966..2181)
                     /locus_tag="PLM74_05000"
     CDS             complement(<1966..2181)
                     /locus_tag="PLM74_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42477.1"
                     /translation="MGQASLRTDELCQGALARSADMPALSLADRADRADRASRAGRAG
                     MGLWSRLTSHRGGRLGLIIVLAYVAIAV"
     gene            complement(2217..3245)
                     /locus_tag="PLM74_05005"
     CDS             complement(2217..3245)
                     /locus_tag="PLM74_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HON42478.1"
                     /translation="MIRLSRLQVKVIERIAATIPLALAVALAAFVLMRAIPGDPVDIM
                     MGEGASSSGEEVAALRAQLGLDEPIHRQLSSYLKRLFNGDLGYSITRSRPVAEMIREA
                     LPATIELAVYAILFALALGIPVGVVSALRHNTAFDRVSTAVSFIGISLPAFWLGIVGI
                     LVFSMTLRWLPTSGRIPYGLEPRHITGLLTLDALLTGNMPAFGSALRHICLPAVTLGL
                     GVAAVIARITRSSMLDVLGSDFVLFARAKGVPERAVTIRHALRNAMIPTVTLVGLQFG
                     SLLGGNMIVETIFGWPGLGRVVVDAIFARDYPVVQASVMVYALTFVMVNLITDLIYTV
                     LDPKMALQ"
     gene            complement(3242..4783)
                     /locus_tag="PLM74_05010"
     CDS             complement(3242..4783)
                     /locus_tag="PLM74_05010"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HON42479.1"
                     /translation="MTAISICVLLLCMAIPRPAAGGDSTKALSVIVDTIADRSDEAQA
                     IAEYLRAVGVSAEVRVWEWSALKERILAGERQMYLTDWGSAYFDPFDLAVPKLKTGDR
                     GNYSFYSNPRFDAALDTALAVRDHEARRRAYFEAQKILRPDAPWVFGYSLRETEAARA
                     DVLDWQPSMDSRMNLHDVGLKRGDTIVVGMAADGMLTLDPAAYRDRATETVIRNMFDG
                     LVTRTVDGRIVPEIAESWSAVSDTEYIFRIRRGVKFHNGDALTADDVVFSFDRVIRDG
                     AMGGRTSPRKGLLGPLAAVEKVDSHTIKVRLSSPFPVLLQAMVHVQIVPRDYLMRVGD
                     DAFARKPVGCGPFRFVEGRLDDKVVMERFDGYYGGSPDLPPVGSARVTRAIFRMMPDP
                     TVRVAALKTGEVHIIQTVPAHLASSLARDRNLQVKSAEGTRVYCIEMNCARPPFDDVR
                     VRVAVNHAIDWDAILTAIYLGSATRLATAFLPSGFGYDAELAPYSYDPNKARALLAEA
                     GYGGR"
     regulatory      complement(4968..5112)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            5287..6372
                     /locus_tag="PLM74_05015"
     CDS             5287..6372
                     /locus_tag="PLM74_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HON42480.1"
                     /translation="MTENSPGLPLVSAVDVTKHFISAESFLARRLGGATTQRTLAVDG
                     VDLHILAGQTVGLVGESGCGKSTFGRTLVGLTEPTSGQVFYEGSPVWGPNRLSRRDLK
                     HLAQIIFQNPYSSLNPRNTVRQTIGAALAAKGVRPGDRELEARSLITRVGLTPGHLDR
                     YPHQLSGGQRQRVGIARALAMRPRFIVADEPLSSLDVSVQAQIIALLQDLQREYGLSY
                     LLISHDLRVVYHMSDTVAVMYRGQIVELASADDLFSQPTHPYTQQLLSAIPGSGHRLS
                     RRRSRQIRAPGADRDSCGREACAAEPPALLGCRFAHRCSVATRKCVSEPADLAPLSKY
                     SESCPHLVRCHNPMPAPADAEGGCPPQ"
     gene            6369..7211
                     /locus_tag="PLM74_05020"
     CDS             6369..7211
                     /locus_tag="PLM74_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42481.1"
                     /translation="MTRIAAALTLALLITTCICSPTVVWGASSDPAGVPHSRAQDILL
                     KMLQAQENLTSFEGTAEAKVYIGGRNVLTTSVGLVIERPNHVAVKWFGMTIRPRHGLI
                     FVDPQIFVSNDYTLSVLSAPAESSRDASSRSERWVISAVSSGSSRPQLSWVLYVEPDT
                     WLIRRAQVTMGATDRTTGTAGVGAAVGAGVGVATGTASAGGETAIIEADYRQAGYRRW
                     EPVRLSAQGRMVLEEFLPNFLVNIVAGRGSRSTSDAVSGSAPFVLLEFGASRPGAPMR
                     GQSK"
     gene            complement(7274..7546)
                     /locus_tag="PLM74_05025"
     CDS             complement(7274..7546)
                     /locus_tag="PLM74_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HON42482.1"
                     /translation="MTKSDLVDSVAAKAGMTKKDSARAVDAVFDTIKEQLKTGDKVQL
                     VGFGSFEVKQREARVGRNPKTMEEIRIPARRVPVFRPGKELKESVM"
     gene            complement(7627..>8367)
                     /locus_tag="PLM74_05030"
     CDS             complement(7627..>8367)
                     /locus_tag="PLM74_05030"
                     /inference="COORDINATES: protein motif:HMM:NF021089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HON42483.1"
                     /translation="DWVAELDEAHPVRAGFIAGVRQAVDSVDKVRDVQGAVQTLGLTK
                     NAQSAAVTLLDLGTGRVLIELTAPRALFYTVISEMIGAEVEGDSGVMRMMRGLTHAKR
                     EYDKVATALDQVRSKGYGIVMPFLEEISFQEPDIIRQGSRCGVRLKATASSIHMIRAD
                     IKTEVTPLVGTEKQSEEFARRMAEEFAEDPSKIWQTEFLGTPMQDLIREGIEAKLAHM
                     PVHAQQKLQETLEKIVNEGSGGLICIIL"
BASE COUNT         1659 a         2716 c         2357 g         1625 t
ORIGIN      
        1 tcccgacctc ttgcacccca agcaagcgcg ctagccaaac tgcgccacgc cccgaccgtc
       61 tcacaatggc cattatagca gagatcatcc gcccatacaa cccccgattt gccgcccgat
      121 ttgccgcatg aattgcgccg tgccgggcat cccggattgt ggagcatggc acctgagcat
      181 ccgtcccgac actgcttgca caggcggctc ttcggcttcg caccggtccg ttcgatgccg
      241 acacctgggg gcgaaagcgc agccagaccc caaagaagtg aggtcaggca cctctcccgg
      301 tattgcacgc agcggttgcc tgctgcctaa gcgcggcata gacagcagaa gtccgcgggt
      361 gtacgggtga agagggtctt ccatgattgc gtctgccggc ccccactcca cgatctgccc
      421 ggcgtacatc accgcaatgt tgtcgcagaa gtcgccggcc agcgccagat cgtgtgtgac
      481 tatgattatg gctgtgccca tctcgcagtt gatgtcttcc atcagctcga gcacttgggc
      541 ctgtatggtc acgtccagcg cggtagttgg ctcatcggcc aacagcaggc gcggcttgca
      601 cgcaagggca atggctatca tgatcctctg ctgcataccc ccgctgaact ggtgaggata
      661 ctccagatga cggtggtcgg gagaaggaat gcccacccgc ctcatcagct caatcacgta
      721 atcgcgttct tcttcacgct gcctccgtgc ctgccacagg cgcgaccgga ggcaagcccg
      781 tccaagcccg cgtctccctg cgcccgacgg catcatcgca tgtacccgca gcgattcgga
      841 gatctgctcg cctacacggt atgcgggatt cagcgttgtc atggggtctt ggaacaccat
      901 ggcgatctcc ttgccacgta cctcccgcat ctcgcccgga gatagcccga gcagatctac
      961 accgcccagc cgcacggagc cacgggcgat gcgcccgggg cggggcagca atcgcattat
     1021 cgacagcatg agcgcactct tgccgcaccc cgactcgcct actatgccca ggacttctcg
     1081 aggcgccaca ctaagactca cgtctctcac agcgcgcaca tccccccggg cagtaggata
     1141 cagtgtgtgt agatcacaca catcaagcaa catgccggtt tcagatgcca tactgaatgt
     1201 tatccctcca ttatcttggg gtccaggacg tcgcgtagcc cctctccaag caggttcatc
     1261 cccagcacca acagagccag cgcaagtccc gggaaggtgg caacccatgg tgaggtgagc
     1321 atgtagcgtc gtccgtcatt tatcatagat ccccatgcgg gcatccgcgg cggaacgccg
     1381 aggcccagga aactcaatga tgactccatg acaatcatgt ttcccatgcg cagggtgccc
     1441 agcaccagga tggagccggc gatgttgggg agtatctcct gcacgcagat cgctccgtcg
     1501 gacctgccca acactcgggc cgcctctacg tattcgcgat tcttctccga catgactagg
     1561 gcacgtgaca gcctgtagaa ctcgacccat cccttgaagc ttagggccca taccagattc
     1621 ccaaagccgg ggcccatgac cgacatcgct cctatcacaa agatcaggta agggaatgcc
     1681 agcagcagtt ctgtgaaccg agcgagcgcg cggtcgagta cgccgccgta gtagccggca
     1741 accacgccca gccccacgcc aagaacggca gagatggagg tagcagccag gcccacgagc
     1801 atcgataccc tggcaccgtg catgatccgg gacaggatgt cgcggccgag ctggtcagtg
     1861 ccgaggaagt tgcccgttct gattgcggga ggtgctaacc tcaccgccag gttgaccgcg
     1921 cctggaggat acggcgcaac cacaggggcg gctacnnnnn nnnnntacag ctatcgccac
     1981 ataggcgagg actattatca agcccagcct gccccctctg tgcgacgtca gtctggacca
     2041 gaggcccatt cctgccctgc ccgccctact cgccctgtcc gctctatccg ccctgtccgc
     2101 caggctcaag gcgggcatgt cggcggagcg tgccagcgcg ccctggcata actcatccgt
     2161 tcgcaacgaa gcctgcccca aaaatctcac ctccgcatga gcgcaagcaa ctcgagtcac
     2221 tgcagcgcca tcttggggtc caacaccgta tatatcaggt ccgtgatgag gttcaccatt
     2281 acgaacgtaa gcgcgtacac cataaccgat gcctgcacca ccgggtagtc tcgagcgaag
     2341 atcgcatcca ccactacccg cccgagaccg ggccagccga atatggtctc gactatcatg
     2401 tttccgccca ggagcgaacc aaactgcagc cccactaagg tcactgtggg aatcatggca
     2461 ttgcgcagag catggcgaat ggtgactgcc cgttcaggta cgcccttcgc cctcgcaaat
     2521 agcacgaagt cggatcccag cacatcgagc atactggagc gcgtgattct ggctattacc
     2581 gccgcaacac ccaacccaag cgtaacagca ggaaggcata tgtgccgcag agcactgccg
     2641 aaggcgggca tgttgcctgt gagcagtgca tccagcgtca gaagaccagt gatgtggcga
     2701 ggttccagcc cgtatgggat gcgcccagat gtgggcaacc agcgaagcgt catcgaaaag
     2761 acgagaatgc cgactatgcc aagccagaac gcaggcagcg atatgccgat gaacgacacg
     2821 gccgtactca ctcggtcgaa cgccgtgttg tgcctgagcg ccgacaccac ccccacggga
     2881 atgcccagag ccagagcgaa cagtatggca tacacagcga gttcaatggt ggcaggcagc
     2941 gcctcccgta tcatctcggc caccggacgc gatcgtgtta tcgaatatcc cagatcgccg
     3001 ttgaagagcc gcttcaggta gctagacagc tgcctgtgga tcggctcatc cagcccgagc
     3061 tgggcccgga gagcggccac ctcctcgcct gaagagctgg ccccctcgcc catcatgatg
     3121 tcaacagggt ctcccgggat cgccctcatc agcacgaacg cggccagcgc cacggccagt
     3181 gccaacggga tcgtggcggc tatgcgctca atcaccttga cctgcaaccg gctgaggcgt
     3241 atcatctgcc accataccct gcctccgcca gaagcgcgcg ggctttgttc gggtcgtaac
     3301 tgtacggtgc tagctcggcg tcatacccaa atccgctggg cagaaaggct gttgcaagcc
     3361 tcgtcgcgct gccaaggtat atcgccgtga ggatggcatc ccagtctatt gcgtggttta
     3421 ctgctacgcg tactctcaca tcgtcaaagg gaggtcgcgc gcagttcatc tcgatgcagt
     3481 acacgcgggt cccttcagcc gacttcacct gtaggttgcg gtcgcgcgca aggcttgacg
     3541 ccaagtgtgc gggaaccgtc tggatgatat gcacctcgcc tgtcttgagc gctgcgactc
     3601 gtacagttgg atctggcatc atgcggaata tcgccctcgt cacgcgggcc gaacccacgg
     3661 gcggaagatc gggcgaacct ccgtaatagc cgtcgaatcg ctccattacc accttgtcat
     3721 caagacgacc ttcaacgaac ctgaacggac cacagcctac gggcttgcgg gcgaacgcat
     3781 cgtcgcccac tctcatcaag tagtccctgg gaactatctg cacgtgcacc attgcctgga
     3841 gcagaaccgg gaagggcgac gacaggcgga ccttgatggt atggctgtcg accttttcca
     3901 cagcggccag cgggcccagc aaccctttcc gaggtgaagt cctgccgccc atggcgccgt
     3961 ctcggatcac tcggtcgaag ctgaagacca catcgtccgc agtgagcgcg tcaccgttgt
     4021 ggaacttgac gccgcggcgg atcctgaaga tgtactcagt gtctgacaca gcgctccatg
     4081 attccgctat ctccggaacg attcgaccgt ccacggtgcg tgttacgagt ccgtcgaaca
     4141 tgttcctgat caccgtctcg gtagctcgat cacggtaagc cgcgggatcc agcgtcagca
     4201 tcccgtctgc cgccatcccc actacgatgg tgtcgccacg cttcagtccc acatcgtgca
     4261 agttcattct ggagtccatc gatggttgcc aatcgagcac atctgcccgg gcagcctcag
     4321 tctcgcgcag cgagtagcca aatacccatg gtgcgtccgg ccgcaggatc ttctgtgcct
     4381 cgaagtatgc acggcgccgc gcctcatggt cgcgaacagc aagcgcggtg tcaagcgctg
     4441 cgtcgaaacg tgggttgctg tagaaggagt agttgcctcg gtcgccagtc ttgagcttgg
     4501 gcactgccag atcgaacggg tcgaagtagg cgctgcccca atcggtgagg tacatctgcc
     4561 tttcaccggc gaggatcctc tctttgaggg cgctccactc ccacacccgc acttctgccg
     4621 acacaccgac ggcgcgaaga tactcggcga ttgcttgtgc ctcatctgaa cggtcggcaa
     4681 tagtatccac aatcaccgag agagccttcg tggagtcgcc gcctgcagcc ggcctaggaa
     4741 ttgccatgca cagcaacagg acgcatatgg aaatcgctgt cacccaacgg tacctcatct
     4801 gcatggtcac ccaccgtcca tactggattt ccagatagac tggacgtgcg atctgccgag
     4861 cggggtttgg gatgagccga gactacttgg gcaagcgcaa gatgaagaga caggggcacc
     4921 ccgtgcctaa atcgaaaagt gcccagggca cacccgggca tagatgggcg tcccttcatt
     4981 tcgcttgcga ggttagctga cgggctcggg ccgaaggtga tagccctacc tgcctatttg
     5041 caggattcgc cccatccacc gggatttggt tcccccgctc ttccctcagg gaagattcag
     5101 cggatgcgcg ccgggaggcg cacatcgttc acgcacgtat tcagctgtcg ctaccttgga
     5161 tgctatcatc caagagaggc gatgtcaacc gtcgccaagg tgcaacaatg cgtacggcct
     5221 gcgtattggc tgtttaggac agacctggaa cttaggtgct ataatgaatg cacggaggtg
     5281 ttggccgtga ctgaaaacag ccccggtctg ccgctggttt ccgctgtcga cgtcacaaag
     5341 cactttatca gcgctgagtc gtttcttgcg cgccggctgg gcggcgcaac tactcagcgc
     5401 acgctggctg tcgacggcgt tgacctccat atactcgcag gccaaaccgt tggccttgtg
     5461 ggcgaatccg gctgcggcaa gagcactttc ggtcgcactc tagttggtct gaccgagccc
     5521 acatccggcc aggtcttcta cgaagggtcg ccggtttggg gtcccaatcg cctcagtcgc
     5581 cgcgacctga agcacctggc gcagattatc ttccagaacc cgtacagctc actcaacccg
     5641 cgaaacacag ttcgtcagac catcggagca gctctggccg caaaaggggt tcggcctggc
     5701 gaccgcgaac ttgaggcgcg atcgctcatc acgcgagtcg gcctgacacc aggccacctc
     5761 gatcggtacc cgcaccagct cagcggcggg cagcgccagc gtgtcggcat cgcgcgcgcc
     5821 ctggcgatgc gtccgcggtt catcgtggcg gacgagcccc tctcctcgct ggatgtatcg
     5881 gtgcaggccc agataatcgc tttgctgcaa gaccttcaga gggagtacgg gctgtcctat
     5941 ctgctcatct cgcacgatct ccgggtagtg taccacatga gtgatactgt ggccgtgatg
     6001 taccgtgggc agattgtgga attggcctcg gccgacgacc tcttctcgca gccgacccac
     6061 ccctacacac agcaactgct gtcggccatc cccggtagtg ggcacaggct gagcagacgg
     6121 cgctctcgcc agatccgcgc cccgggcgca gaccgcgact catgcggtcg ggaggcatgc
     6181 gctgctgagc ccccggccct gctcggttgc cgtttcgctc accgctgttc ggttgcgacg
     6241 cgcaaatgcg tatcagaacc cgcggacctc gctccgttgt cgaaatactc cgaatcttgc
     6301 ccacatcttg tgagatgcca caatcccatg ccggctccgg cagatgctga gggagggtgc
     6361 ccgccgcagt gacacgcatt gcagcagcgc taacacttgc gctcctcatc acaacatgta
     6421 tttgttcgcc aaccgtggtc tggggggcat cctctgatcc ggcaggcgtc cctcattcca
     6481 gggcacagga tatactgctc aagatgcttc aggctcagga gaacctgacc tcattcgaag
     6541 gcacagccga agccaaggtc tacatcggag gtcggaacgt gctgactacc tcggtgggcc
     6601 tggttatcga aaggcccaac cacgtagctg tgaaatggtt cggcatgacc attaggcccc
     6661 ggcatgggct gatattcgtc gatccacaga tatttgtgtc aaatgactat actctttcgg
     6721 ttctgtcggc gcccgccgag agcagccgcg acgcatcgag ccgatccgaa aggtgggtta
     6781 tctcagctgt atcgtcaggt tcgagtcgac cccagttaag ctgggttctt tatgtagaac
     6841 ctgatacctg gctcatcagg cgagcccagg tcaccatggg cgccaccgac cggacaactg
     6901 gaacagccgg ggtcggcgcc gcggtgggcg ccggggtcgg cgttgccacc ggcacggctt
     6961 cggcaggcgg cgagaccgcc atcatagagg cagactatcg tcaggccggc tacaggcgat
     7021 gggagcccgt ccgactctcg gcgcaagggc ggatggtact cgaggagttc ctgcccaatt
     7081 tcctcgtcaa catagtcgca ggtcgcggca gtcgcagcac aagtgacgcg gtaagcggct
     7141 cggcaccatt tgtcctgctg gaattcggcg caagccgccc aggagcgcca atgagggggc
     7201 agagcaaata agcgtagccg ctgttacggt ctacgcttct tgctcggcaa cccgcacatc
     7261 tcaccacgta ttgctacata accgattcct tcagctcttt gccgggtctg aaaacgggca
     7321 cccttcgcgc tgggatgcgt atctcctcca tggtcttggg gttgcggccc actcgggctt
     7381 ctctctgctt cacctcaaag ctaccgaacc cgacgagctg caccttatca cccgtcttga
     7441 gctgctcctt tatggtgtcg aagactgcgt ccaccgcccg ggcgctgtct ttcttagtca
     7501 tcccggcctt ggcggcaacc gaatcaacca ggtcagactt ggtcatgcgt tctgccccct
     7561 tccaccagta tgttttccag cctcgcacgc cattattact tgaggcgcag cgagtcgggg
     7621 gccatgctac agtatgatgc agatcagccc gccgcttccc tcgttgacga tcttctccag
     7681 cgtctcctgc agcttctgct gggcatgaac gggcatatgg gccagtttag cctctattcc
     7741 ctccctgatc aagtcctgca tcggagtgcc gagaaactcg gtctgccata tcttggacgg
     7801 gtcttccgca aactcctcgg ccatgcgtcg cgcgaactcc tcactctgct tctccgttcc
     7861 caccagcggc gtgacttcgg ttttgatatc tgcgcgtatc atgtggatag atgacgcagt
     7921 ggccttcagc cgcacaccgc acctgctgcc ctgcctgata atgtcgggct cttggaacga
     7981 gatctcctcc agaaacggca tcacgatacc gtagcccttc gaccgcacct ggtcgagggc
     8041 ggtggctacc ttgtcgtact ctcgtttcgc gtgggtcagc ccgcgcatca tccgcattac
     8101 gccggagtcg ccctcgacct ccgctccgat catctccgaa atgaccgtgt agaacagcgc
     8161 cctcggggcg gtcagctcaa tcagtacgcg accggttccc aagtcgagca gggttaccgc
     8221 cgcagactgg gcgttcttgg tcagcccgag cgtctggacc gctccctgaa catctcggac
     8281 cttatctact gagtccacag cctgacggac gccggcgatg aacccggccc gaacgggatg
     8341 cgcctcgtct agctcggcaa cccagtc
//
LOCUS       DAOTAC010000085         8356 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_7275_length_8356_cov_4.826166, whole genome shotgun sequence.
ACCESSION   DAOTAC010000085 DAOTAC010000000
VERSION     DAOTAC010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 8356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8356
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7275_length_8356_cov_4.826166"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            653..2749
                     /locus_tag="PLM74_05035"
     CDS             653..2749
                     /locus_tag="PLM74_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42484.1"
                     /translation="MCVFILLLATIAVGWLALRSNLSAIGSDVAGAVLSRAFGGADVR
                     VGSVTTQGIRSLAMSGVQLGDGLRAKRVLVAYDFRSLLRFRTSPLLSVTAIRAEGVEA
                     DWIALQKYMGRPESARAASGSQPRSVSGSAPGTGGSKAAAGFAGTISVRDAVVIVGRS
                     DEADGGYPAESKVELEDIRLEPLQRDQVDQVDRVAAWSVTVARASYYDPQQPGRPVEL
                     AGVRGAVALSKDALNVHGFSSRLLGGTVQAGFTVVGAAEGSPAAPRVVGQATLTGVSY
                     HGWAFTGKIATCDDGRIRVDGDVSSERLSARLDGTAAVPGFTPGTASLSADGVLNIKT
                     MDIGESRIRDMRLEVSAERMPGNDHGLGDIVIRIGAQKATLGQIAQALGKPIPAQGWV
                     KWCADAKIGSDGRTFVRIGLADGVVRADGWPDEWPHEFTAVSGAIEVSTVVKTFGDVG
                     PDGVVASAGASASRSGSSEGKPAFSVPGVRAKLGGGEVGLSGSPQSWRVTASGVRVDH
                     QLVSGLVDADLVLRAGKRLLLSGRAAVRDARMDLVVAGVQSGVPVPEADLDIDVEVGE
                     RVMVVRGESWAWALPGTVHVQGSTKRPTASGSLNLSSGQIDLYGVPLEVVSGGVHFTG
                     MPGDPPKFALVARDLASGLPAVISINGVPGDMHISVKPEEGPEEEVLEGDLLMKLLLA
                     RLRLYMLSGLGRALAN"
     gene            3048..4598
                     /locus_tag="PLM74_05040"
     CDS             3048..4598
                     /locus_tag="PLM74_05040"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HON42485.1"
                     /translation="MSIKIGDQLTEKAVEESFTAIDSLGWFADLGANTVPYLGGVKLI
                     VLVREFPVISRITVSGNKLIPTEELLGLMTTKADSQLNSSQLRSDLEALGKHYQDKGY
                     WASIEPSLSEDGGLTLHLVEWTVADVKISGNEKTKPQVVWRAIQTKPGDYINVNKINE
                     DRRKIYLLGAFENVEVTVEPVQGRYEYVINYVLTERKTGIANLSLAYSSADGLMGFIE
                     LGDQNFLGNAQTVNVKAEFAKGKSNYELGFYEPWVGKTGKTSLGFNVFSKSSEKKFTQ
                     EPEEGQEPPEAIKYTESRTGGDVTVGRTLSLNTRAFLKFKMDNAVTRAVNSDDQDVIP
                     KGGITRSLTVSAVNDTRDDLWSPTSGHRLTGSVEYAGGMLGGDHSFTKVEGEGSAYFQ
                     VRDGHVLAGRISSGFGLSALPEQERFRLGGAETVRGYKYGDIEGDRMALANAEYRFRI
                     TKGLQGVVFCDAGQAWNAQDSFTLNGAKVGYGVGVRISVPVLGMIRIDYGIAEGHGQA
                     YFSFGQTF"
     gene            4807..5262
                     /locus_tag="PLM74_05045"
     CDS             4807..5262
                     /locus_tag="PLM74_05045"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HON42486.1"
                     /translation="MFKNLPKALVTLFICTLLVGLGLGLTNLAVSRAAGESVVIGKLD
                     GARVGQEFPAMKTAWENVAKQKAALEKELNKKAEGQDEESQKALLDQYNKKYNDYARE
                     QLDPAQKALETAIAAVAKEKGVTVVLDSSVVHLGGVDITEAVLKKGGAK"
     gene            5369..>8356
                     /locus_tag="PLM74_05050"
     CDS             5369..>8356
                     /locus_tag="PLM74_05050"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HON42487.1"
                     /translation="MRILDRYVVREFMGPFILSISAFIIIMLSGQLFMLVDFIVQKNV
                     PVLAVVRMLLYSLPGIVVQVLPVAVLFAVLISLGRLARDSELSTIRAAGIPFTRIVLP
                     LILMGAAVSAGGFWLGETVVPWASHKSETIVREIILKDVLPSVEQDIFFAAPGGRQFY
                     VGQVNKDLGTMERIMVYETEHGSFPRIVSARSGSVGEGKWTLQDGVIHELDAAGHVAM
                     SMSFEKLEIMMDSTMETFVSSQKTPREMTRKELKSHIDLFERSGIRVEGLRVEYHLKL
                     AEPMAALVFAVIGAPLVARSPRRAGGYFGVVAAAVLSFAYFVVQAVTRSVGMKGMIQP
                     VTAVWIPNAAFLLPGLVMLLKVDRPYVFSGRRAGGAGSGSSSGPGQGRALRSFLVACA
                     LTTALVLALSLLGGVPVTAATEPVRVCAWQMVFDGARNAWELSGEVEIIYKDTVIHGD
                     HAKIDAGAKRAVIAGSVSVVRGDETLKGEVATYNMSTGDVEIEQAWAKLKDDQITGFL
                     FASGAELTKKDDYLRLSEGFVTTCDLDKPHWRIEVRELEVYADDRIVLRSVSYYEGNV
                     KLLSLPRLTIPLKEDERLELPKIGYGAVEGWYVKSKYNYRLSDVSSGSLFADYFELLG
                     PAAGVRQQLKLGAAGVLTAYVYELWNRASGNGDTTFELSHSVDLPADVKAMAGYRYRD
                     YSVLGGASSPTVERTYTLNLDRRVEGANTSFAYTRRDVESAQNSEALSLSFRHSSTLP
                     LAIKLTSDVSYRRQVKGEDVTQNVLNYRVQAARTLGGFSLTGLAQAQVYVEEESTALP
                     DVKPPPWKALTRLPEFTAVSGRIPIGKTPLSVTLKGVLGRYTEDAIRGGIRSEVTSGK
                     AEADVTLRMDPQNVNKWLSVDGSVRGVLDAYGANGTQAAMGYSAGVQVKPAAGTTLRV
                     SYDQMSVAGASPFRFDVINPSSKVGARLTSTLGPLRLSLATAYDFKYQTYDTLTLAAQ
                     AEVSKGISVELSGGYDIRQASPKS"
BASE COUNT         1705 a         2197 c         2737 g         1717 t
ORIGIN      
        1 aaatgccggt atctccgaaa ctattgccga aaactgacga tatcccagct ccagccgttt
       61 tgccctgcga tcatcatggc gggaccagct aaaaggccgt ttcagcgagg atgacccttt
      121 ctgccgttat tctgtctggc aatgcctgct cgaacttgca cgtcgcggtg tctataagaa
      181 ctacacaggc ctggggcggg ttgaggtgga cgccgacagc ggggccgacc atttcgggcg
      241 gagcacgccg gggcccgcgg cgaccgtttc ggtccaggcg acctagctct gtccaacgcc
      301 tccagagggg agaaaaccta ccaagatacc cttgggggta tctgggcaac ttctctcgct
      361 gctgcgggct tggcgcagtc cttcagcaca accgggcccg ggttccccga aactgaccga
      421 cacgcggtcg cggcagtccg cattggattg actgcggtat ttgtgcgcga caggctatgg
      481 cgcctgtcga tacgcgcctt ggttaccact tcgcaccata cgcaacatca tatgcccagc
      541 taaccgccgt ttcactcggc gaccgccgaa ggaaacggct atgcgccgtc gaagttgaca
      601 gcggtggtga atgcttgtgg cgcgtatcag ccgcctgcgc atgaggcgct caatgtgcgt
      661 tttcatactt ctgctggcca cgatcgctgt agggtggttg gcgcttcgaa gtaacctgtc
      721 cgcgatcggg tctgacgtgg ccggggccgt tctttctcgc gctttcgggg gcgccgatgt
      781 gcgggttgga agcgtaacga ctcagggcat acggtcgttg gccatgtccg gtgtgcagct
      841 aggagacggg ttgagggcga aaagggtgct ggtcgcttac gacttccgct cactgttgcg
      901 gttccgcacc agtcctctgc tgagtgttac tgcgatacga gctgaaggag tagaggcaga
      961 ttggatcgcc cttcagaaat acatgggccg tcccgaatct gcccgcgcag cctccggtag
     1021 ccaacctcgc tcagtttcag ggtcagcccc gggcacagga gggagcaaag ccgctgctgg
     1081 ttttgcaggc acgatctcgg tccgtgatgc cgtcgtcata gtcggtcgtt cagacgaggc
     1141 cgatggcggc tatccggcag aaagcaaggt tgagctagag gatatccgac tggagccact
     1201 tcagcgcgat caggtggatc aggtcgaccg ggtcgctgca tggtcggtca ctgttgcccg
     1261 cgcgtcatac tatgatccac agcaacccgg ccggccggtg gagttggctg gtgtccgcgg
     1321 cgcggtcgca ctatcgaagg atgcgttgaa tgtacacggt ttttcgtcac gactgcttgg
     1381 gggaacggtg caggccgggt tcacagtggt gggcgcagcc gaagggagtc ctgccgcgcc
     1441 ccgcgttgtg gggcaggcta ccttgacagg agtctcatac catggatggg ctttcactgg
     1501 caagattgcc acgtgtgatg acggaaggat acgggttgat ggtgacgtca gctccgaacg
     1561 cctgagcgct cggctggacg gaactgccgc agtcccgggc tttactcccg gcaccgcgag
     1621 cctgtctgcc gatggggttc tcaacataaa gactatggac atcggcgaat cgcgcatccg
     1681 ggacatgagg ctcgaggtgt cggcggagcg catgcctggc aatgaccacg gactaggcga
     1741 tatcgtgatc cgcataggcg cccagaaggc cactctcggg cagattgccc aagcgctggg
     1801 taagcccatt cctgcccagg ggtgggtcaa atggtgcgca gacgccaaga tcggctccga
     1861 tgggcgcacc tttgttcgca tagggctcgc agatggggtg gtacgcgccg acggatggcc
     1921 ggacgaatgg ccccacgagt ttacggccgt ttcgggcgcg atcgaggtat ctaccgtagt
     1981 caagaccttc ggagacgtcg gccccgacgg cgttgttgcg tcggccggcg cgtctgcaag
     2041 tcgaagcggg tccagcgagg gcaagcccgc tttcagcgta cccggggttc gggcaaaact
     2101 gggcggcggc gaggtggggc tatcgggctc tccgcagtcg tggcgcgtca cggcatcggg
     2161 ggtcagggtc gatcaccaac tcgtgtcggg gcttgtcgat gccgacctgg tgctgcgagc
     2221 cggaaaacga cttctcttga gcgggcgcgc ggcggtgcga gacgctcgga tggatctggt
     2281 ggtagctgga gttcagagcg gggttccggt tccagaggcg gatctggata tcgatgttga
     2341 ggtcggcgag cgtgtgatgg tcgtgcgggg cgagtcgtgg gcatgggcac tgcctggaac
     2401 ggttcacgtg caaggcagca ccaagcgccc aacagcctct ggaagtctga atctgtcatc
     2461 cggtcagata gacctgtacg gtgttccgct tgaggtcgtg tcgggaggag tgcacttcac
     2521 cggaatgccg ggtgacccgc ccaaatttgc tctggtggca cgggatctag cttcaggact
     2581 cccggcggtc atttccataa atggtgtgcc gggcgacatg cacatatcgg tgaaacccga
     2641 ggagggacct gaggaagaag tacttgaagg ggaccttctc atgaaacttc tgctggcccg
     2701 gctgcgtcta tacatgttaa gcgggctcgg acgagcgctt gcgaattgat cacccgggcg
     2761 agcgcagaag gattcgggtc gatggagtag aatagaaaat caagccactg gggtgtagga
     2821 gcgacttcat ctggtcatgt gtgtgatcag tcgggtgaga tgtgggaggg gagattgttg
     2881 aactcaagta gcgccaggag gttctgcggc gtgctggccg ttttggtggt gacgatgctc
     2941 ttggcttcgt cagccgctct ggggcgccgg ccgaaaccct cggcagggta actgccatag
     3001 aagtccaggg aaacgtcaga atcgactcgg atacgatcat taacaagatg agtatcaaga
     3061 taggcgacca gttgactgag aaggctgtcg aggagagttt cactgccatc gactccctgg
     3121 gctggttcgc tgatctggga gcgaacaccg ttccctacct agggggcgtg aagcttattg
     3181 tactggtccg ggagtttccg gtcatcagcc gcattacggt aagcgggaac aagctcatac
     3241 ctaccgagga actcctcggg ctgatgacga caaaggccga ctcccagctg aattcgagtc
     3301 agctcagatc ggatctcgaa gccttaggca agcactacca agataagggc tactgggcga
     3361 gcatcgagcc gtccctgtcc gaagatggag ggctcaccct gcacctggtg gagtggactg
     3421 tggccgatgt gaagatatcc ggcaacgaga agaccaaacc ccaggtggtc tggcgcgcga
     3481 ttcagaccaa acccggcgac tacatcaatg ttaacaagat caatgaggat cggcggaaga
     3541 tctacctgct gggcgctttc gagaatgtcg aggtcacagt ggagccggtt cagggcaggt
     3601 acgagtatgt aatcaactat gttctcaccg aaagaaagac tggtatcgcc aacttgagcc
     3661 tggcctacag ttcggctgat ggcctcatgg gcttcattga gctcggcgac cagaatttcc
     3721 tcggcaatgc ccagacagtc aatgtcaagg ctgagttcgc caagggcaag agcaattatg
     3781 agttgggttt ctatgaacca tgggtgggca agacgggcaa aacctcgctg ggcttcaacg
     3841 tgttcagcaa gtcgtccgag aagaagttca ctcaggaacc agaagagggc caggaaccgc
     3901 cggaagccat taagtacacc gaaagccgca caggcggcga tgtgacggta ggccgaacgc
     3961 tctcccttaa caccagggca ttcctcaagt tcaagatgga taatgctgtc acccgagccg
     4021 tgaattccga tgaccaggat gtcatcccga aaggcggcat aacacggtct cttacggtat
     4081 ctgctgtgaa cgacaccaga gacgacctgt ggagccccac atccggccat aggctgaccg
     4141 gatcagtgga gtatgccggc ggcatgctcg ggggcgatca cagcttcact aaagtcgaag
     4201 gcgaagggtc tgcgtacttc caggttcgtg atggccacgt gctggcggga cggattagtt
     4261 ccgggtttgg cctcagcgcc ctgccggagc aggagcggtt ccgcctgggc ggagccgaga
     4321 ctgtgcgagg ctacaagtac ggcgacatcg agggcgaccg tatggctttg gcaaacgccg
     4381 aataccgatt taggataacc aagggcctgc agggtgtggt gttctgcgat gcggggcaag
     4441 cctggaatgc tcaggactcc ttcacgctta acggggccaa ggtcggatat ggagtgggcg
     4501 tgcggatttc cgtcccggtg ctgggcatga tccgcatcga ctacgggatc gctgaaggtc
     4561 acggccaggc ttacttcagc ttcggacaga ccttctaggt tgtcgagccg accccctgtg
     4621 ggtgtaccgt gtgggtgtac cggcatgggt gtgccgtcgg aatgacagct gtgaccggct
     4681 gttgtataat tgagcgggcg ccgctgtggt cggcgccgtc tctatgccgc gtatgcaggc
     4741 acgaatgcct gtgagcggta tgtcggaatg gattgatcta tacgaacaga agagaggatg
     4801 acctgcatgt tcaagaacct tcccaaagca cttgttactc tgtttatctg cactctcctg
     4861 gtcggtctgg ggcttggcct aaccaacctg gcagtctccc gggctgcggg cgagtctgtt
     4921 gtaataggca agcttgacgg agccagggtc gggcaggagt ttccggccat gaagactgcg
     4981 tgggagaatg ttgccaagca gaaggccgct ttggaaaagg aactcaacaa gaaggccgag
     5041 ggccaggacg aggagagcca gaaagcgctt ctcgaccagt acaacaagaa gtacaacgac
     5101 tatgcccgtg aacagctaga tccggcacag aaggcgctgg agactgctat agctgccgta
     5161 gccaaggaga aaggtgttac tgtagtactt gacagcagcg tcgtgcatct tggcggggtg
     5221 gatatcactg aggcagtgct gaagaagggc ggagccaaat agggcagcac cgcggtttcc
     5281 tgggacgtta gggccgcagt gatgcaaagc gatgcggcat gcggggagat gcggagcgcg
     5341 tctccccgct attgagaggt atcggcccat gaggattctt gacaggtatg tggttcgtga
     5401 gttcatgggc ccgttcatcc tgtcgatatc tgccttcata ataataatgc tcagcgggca
     5461 gctgttcatg ttggtagact tcatcgtcca aaagaacgtg cctgtgcttg ctgttgttcg
     5521 catgctgctg tacagccttc cggggatcgt cgttcaagta ctccctgtag ccgtcctgtt
     5581 tgcggtgctg ataagcctgg ggcgtctggc ccgcgactct gagctttcca caatccgtgc
     5641 cgctggaatc cctttcaccc ggattgtcct tcccctgatt ctgatgggcg ccgccgttag
     5701 tgccggaggg ttctggcttg gagagacggt ggtaccttgg gcatctcaca agtcggaaac
     5761 gatagtccga gagataatac tcaaagacgt ccttcccagc gttgaacagg acattttctt
     5821 cgccgccccg ggcggacgcc agttctatgt gggccaggtg aataaggacc tagggaccat
     5881 ggaacggatc atggtgtacg agacagagca cggatccttc ccgcgcatag tgtcagcccg
     5941 cagcggaagc gttggcgagg gaaagtggac tctgcaggac ggcgtgatcc acgagttgga
     6001 cgctgctggg catgtagcga tgtccatgtc tttcgagaag ctcgagatca tgatggacag
     6061 cactatggag acgtttgtgt ccagccagaa gacaccccgg gagatgacga gaaaggaact
     6121 gaagtctcac atagacctgt tcgagaggag cgggattcgc gtcgaagggc tgagagtcga
     6181 gtaccacctg aagttggctg agcccatggc agcactggta ttcgcagtca tcggcgcgcc
     6241 cctggttgca aggtctccaa ggcgcgcagg cgggtatttc ggcgtggttg ccgcggctgt
     6301 actctctttt gcgtacttcg ttgtgcaagc agtcactcgc tccgtgggta tgaagggcat
     6361 gatccagccc gtgacggccg tgtggattcc caacgccgct tttctactac ccggtctggt
     6421 aatgctgctg aaggtggatc gaccatacgt gttcagtggg cgtcgggccg gcggcgccgg
     6481 ctcaggttca agctcaggcc caggtcaagg tagggcgctg cggtcgtttc tggtcgcctg
     6541 cgccctcacg accgcgcttg ttctggcctt atcgctgctc gggggcgtac cggtgacggc
     6601 agcgaccgag cccgttcgtg tgtgtgcttg gcagatggta ttcgatggcg cacgtaacgc
     6661 atgggagcta tcgggagagg tcgagatcat atacaaggat acggtgatcc acggcgatca
     6721 tgcgaagata gatgccggag ctaagagggc agtgattgcc gggtcggtgt cggtggtacg
     6781 cggcgacgag acactcaaag gcgaggtcgc tacatataac atgtctacgg gggacgtaga
     6841 gattgagcag gcatgggcga agctcaaaga cgatcagata acaggttttc tgttcgcgtc
     6901 cggcgcagaa ctaacgaaga aagacgacta cctgcggttg tcagagggtt ttgtgaccac
     6961 ctgtgatctt gacaagccgc actggcggat tgaagtgcgc gaactcgagg tatatgcgga
     7021 tgaccgtatt gtgttgcgat cggtttccta ctacgagggg aatgtgaaac tgctgtcgct
     7081 gccgcgactg acgattcctc tcaaggaaga tgagcgtctg gagttgccca agatcggata
     7141 tggcgctgta gaaggctggt acgtgaagag taagtacaat tacaggctca gcgatgtctc
     7201 gtccgggtcg cttttcgccg actacttcga gctgttagga cccgctgccg gcgtgcggca
     7261 gcagctgaag ctgggagcag ccggagtcct caccgcttat gtgtacgaat tgtggaatag
     7321 ggccagcggc aatggcgaca ccactttcga actttcccat tctgtggatc tgccggcaga
     7381 tgtcaaggct atggccggct ataggtaccg cgactactca gttttgggcg gcgcgagttc
     7441 gcctacggtc gaacggacgt acacgctcaa cctggatagg cgggtcgaag gggccaacac
     7501 gtcttttgcc tacacccggc gcgatgttga gtctgctcag aatagcgagg ctctgtcgtt
     7561 gtcgttccgt cactcgtcca ctcttccgct tgcgatcaag cttacgtcgg acgtgagcta
     7621 ccggcgccag gtgaagggcg aggatgtgac gcagaacgta ctcaactaca gagtgcaggc
     7681 tgcgcggaca ttaggtggat tctcactcac cgggctcgcg caggcgcagg tgtacgtgga
     7741 ggaggagtcg actgcgctgc cagatgtaaa gccgccgcca tggaaggcgc tgacgaggct
     7801 tcccgagttt acagccgtgt ccggccgcat ccctatcggc aagacgccgc tttcggtcac
     7861 gttgaaaggg gtgttgggcc ggtacaccga ggatgcaata cggggcggga ttcggtcaga
     7921 ggtcacttcc gggaaggcgg aggcagacgt caccctgaga atggacccgc agaacgtgaa
     7981 caaatggcta tcggtggacg gctcggttcg cggagttctc gacgcctacg gagccaatgg
     8041 aacgcaggcc gccatggggt actctgcggg agtccaggtg aagccggcag ccggaacgac
     8101 tctccgtgtg agctacgacc agatgtcggt ggcaggggct tcgccattta ggttcgacgt
     8161 gatcaacccg tccagcaagg tcggcgccag gctgacgtcg acactgggac cgctgaggct
     8221 cagcctggcg acggcatatg acttcaagta tcagacgtac gatacactga ctttggcggc
     8281 gcaggctgag gtttcgaagg ggatatctgt agagctgtcg ggcggatacg acatacggca
     8341 ggcatcgccc aaatcc
//
LOCUS       DAOTAC010000086         8288 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_7342_length_8288_cov_4.917041, whole genome shotgun sequence.
ACCESSION   DAOTAC010000086 DAOTAC010000000
VERSION     DAOTAC010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 8288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8288
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7342_length_8288_cov_4.917041"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1465)
                     /gene="pknB"
                     /locus_tag="PLM74_05055"
     CDS             complement(<1..1465)
                     /gene="pknB"
                     /locus_tag="PLM74_05055"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HON42488.1"
                     /translation="MTGELLGGRYSILDTIGEGGMATVYRARDGFLQRTVAIKVLRPQ
                     YASDAEFRERFRREAQSAAALSHPNIVNVYDVGREDGSNYIVMECVDGRTLNDIIVSE
                     GRLSVDRTADYSAQILEALDHAHANGVIHRDIKPHNILITRDGRVKVTDFGIARAVSA
                     SALTETGRVIGTVNYTSPEQARGAPATAESDIYSLGVVMYEMLTGRLPFAGDTPVAVA
                     LKHVQEDPVPISLLNPSVPPEFERVVMRALAKNPEDRWRTARSFRHAIARARQEIAEG
                     GREAPLSAVGMDREVPPARLLHDEHNITRPFEKTGEAFEVQRDDSKRPKREPGPEGAK
                     SRRFKIWATVAALILLMAGAVFGALHAFREWIQVEEVAVPEFVGLTIPEAEGVARRSR
                     LALDASVKRYDDKVEYNRVISQDPVSGVKRKVHSVVRVVVSLGPEMVTVPDLFNKTER
                     EARFELEGLNIALGEKTDGYHEEVQPGRIIEQQPEAGM"
     gene            complement(1470..2852)
                     /locus_tag="PLM74_05060"
     CDS             complement(1470..2852)
                     /locus_tag="PLM74_05060"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HON42489.1"
                     /translation="MNHRISRLLGAWMVGCAVLALAATYYQAVTADRLAASPANPRVA
                     NVEKGIWRGSIVDRNGEILAESLERGLESRVYRGPGSLAPLVGYAHPTLGRSGLESAM
                     DQWLAGRGWSYSRTGLLSTISQQPDRGYSVRLTIDLRTQRACEAALAGRRGAVVVVDA
                     ASGEVLAAASSPWVHQAALESAWDTVIAREDSPLICRPLAGLYPPGSTIKPAIAGAAI
                     DMGLAGSGTVYDCRGSIRAGGTIISDAAGAHGSIDLQTAIALSCNTAFVHLSLEAGAR
                     LPEALAGFYLCRSLPLDVPTAAAAFSSEAGDDVLGQLAIGQGDLVVTPLHVAMMAAAV
                     VNHGVMMRPRLVTEIRSASGAVVKRSSPGVLCVPMSAEAARIVAGGMRAAVEWGTARA
                     ANLPEYVVGGKTGTAENPHGAPHAWFVGYAQHAGRKVVLSIIIENGGSGGQVAAPLAG
                     SIFRAVLSGR"
     gene            complement(2849..4219)
                     /locus_tag="PLM74_05065"
     CDS             complement(2849..4219)
                     /locus_tag="PLM74_05065"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HON42490.1"
                     /translation="MITGGRSPCRRDAERSLVGIVMLLAGLSGASLSLARSGSVFGWD
                     AVLCFAAGAAFAVAHLLLAMTRFDGDQVIPALVAAAAAFGYASVLRLRPDLALRQLSF
                     IWIGVGVMVVAALWQCKGRASRSGMALSVGAAALLLVTLAFGQERGGARSWIFIGGMG
                     FQPSEFAKIACVLAGADALSSMPTCREAPSQSPSPSGAPHRAGLGQMWASRPVRMVFV
                     IWFVIVALTVLQRDVGTAAIIYISCVALAYVASGNRHVVLGAAALGVAGVGLACKFFS
                     HVGARFAAWSNPWADVAGSSYQIVQAFYALGSGGMLGRGYGSGSPWLIPAAPTDMLFA
                     ASAEEIGLAGSLGVIAIYFVLVGHAYACAMRAQSPRQRLAAVGAANILAVQTFVIIGG
                     NLGVLPLTGVTLPFASYGGSSMIASLALAGILLGSTAECRSVSAKASAVAGGRDGVDG
                     SGGDGS"
     gene            complement(4216..4977)
                     /locus_tag="PLM74_05070"
     CDS             complement(4216..4977)
                     /locus_tag="PLM74_05070"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HON42491.1"
                     /translation="MKCVCATDVGLVREHNEDACFVGENCCAVADGMGGHRAGEVASR
                     MAILAVEQELSRSGPTGFDIERCIKHANATVLDASRHADALEGMGTTLTVATVTSGVL
                     KVGHVGDSRAYLIAADGIEQLTDDHSVVGEMLRSGLITAEEAAAHPKRHAITRALGFF
                     DDLEVDVGEWPVRPGDIVVLCTDGLHDLVCPEEIAAAFRGSRDPSSACHDLVSLARSR
                     GGHDNITIAARVVAESDMPGPASPEPEEAPGGSEP"
     gene            complement(5004..5162)
                     /locus_tag="PLM74_05075"
     CDS             complement(5004..5162)
                     /locus_tag="PLM74_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42492.1"
                     /translation="MIRAALVMFRLACLAVLYWFLYRVCACLISDAQASSVACREGDE
                     KAEEDSDG"
     gene            complement(5159..6238)
                     /gene="rlmN"
                     /locus_tag="PLM74_05080"
     CDS             complement(5159..6238)
                     /gene="rlmN"
                     /locus_tag="PLM74_05080"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HON42493.1"
                     /translation="MISTVDIKSMSREECRNFFESIGEPGYRADQVLEWLYHGQVSSY
                     GDMTNIPRLLRVKLAEVAPFGPLEILARRVSPEGDTVKYLMGLADDNAVEAVLMRHDY
                     GLSLCVSSQVGCPMGCAFCASGLGGLVRDLTCGEIIDQLLAASSDAAERFHGARISSL
                     VVMGSGEPLLNYDNVIKFVRLANWDRGFAIGYRHITISTCGIVPGILRLAGEGIPLTL
                     SVSLHAPDDATRSSIMKVNSAYPISTLVKACNRYAQITGRRVTYEYALIAGVNDSQAH
                     AERLAGLLRGTLCHVNLIPLNPVLETTYARPTPQVVQAFADALESRGIEVTVRREMGS
                     GIDAACGQLRRRWARRRSAKEADPG"
     gene            complement(6347..7282)
                     /locus_tag="PLM74_05085"
     CDS             complement(6347..7282)
                     /locus_tag="PLM74_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012342771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HON42494.1"
                     /translation="MIKLVVINLLASTVYLAAPLILTAIGGVYSERAGVFNIGLEGMM
                     LSGSFVAVLFSYMTGNPWLGFWMGAVAGALIATIHAVVSIRYRANQTVSGVAINVLAT
                     ALTGFLLRAIFNRAGQTERVAKLSNWVIPFLRDVPVIGQVLGRNTPPVYLAFIAVIVG
                     HIVLFKTPLGLRIRACGEHPEAADTVGVNVIGIRYLCVIISGFLAGMAGSTLSIGQLS
                     FFREGMSSGRGFIALAAVIFGKWTPLGAMWAALLFALADAVQLTAQTWGFRAIPQEFL
                     LMFPYIVTMAALAGVIGRSTPPAAGGRPYVRRGGA"
     gene            complement(7300..>8288)
                     /locus_tag="PLM74_05090"
     CDS             complement(7300..>8288)
                     /locus_tag="PLM74_05090"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HON42495.1"
                     /translation="TAYEALLKGCFGSPVNISETIVYTTPLIFTGLSIAVAYRAGLLN
                     IGAEGQIIVGMLAAAVVGVSGSGLPRIIHLPLTVLSGAAAGAIWAAVPGYLKAKLGVH
                     EVVNSIMMNYIALFLSHYLVNGPIKDPNIAAPYSAEIADSAKLMRFFGDFHSEFRVNT
                     GILIAIGAAVLVFYILYKTTTGYEIRAVGANPDAARYAGMNVSGAMAISMLLAGAMSG
                     LAGAVQVCAIQYKFLDLFVFEGFGFDGIAVALVGKGNPFGVIGAATLFGTLSRGSQMM
                     QGMAHVPKEVAGIVQAAIILLVAAEGLVRGWLRIPGIRARARRATAAGKEEA"
BASE COUNT         1702 a         2817 c         2291 g         1478 t
ORIGIN      
        1 acatgcccgc ctccggttgc tgctcaatta tcctgccggg ttgcacttcc tcgtggtacc
       61 catcggtctt ctcgcccaga gcgatattga gcccctccag ctcgaaccgt gcctcccgtt
      121 cagtcttgtt gaacaggtcg ggcacagtca ccatctccgg gcccaggctc acgaccacgc
      181 gtaccaccga atgcaccttg cgcttcacgc cggacacagg gtcctgcgag attacgcggt
      241 tgtactctac cttgtcatcg tagcgcttga ccgatgcatc gagggcgagc cggctgcgcc
      301 tcgcgacccc ttcagcctcg gggatagtca gcccaacgaa ctccggaaca gcgacctcct
      361 ccacctggat ccattctctg aatgcgtgaa gagcgccaaa gaccgcgccg gccatcagca
      421 atatgagtgc cgccacggtg gcccagatct tgaagcgccg tgactttgcg ccttcgggtc
      481 cgggctcccg cttgggacgt ttggaatcgt cgcgttgcac ttcaaaggcc tcccctgtct
      541 tctcgaacgg cctcgtgata ttgtgctcgt cgtggagcag gcgcgccggc gggacctcac
      601 gatccatgcc cactgccgaa agaggcgctt ctctcccacc ttcggctatc tcctggcggg
      661 cacgggcaat cgcgtgcctg aacgaccgcg ccgttcgcca tcgatcctct gggttcttgg
      721 ccagcgcccg catgactaca cgctcgaact cgggcgggac cgacgggttc agaagagaga
      781 tcgggaccgg gtcctcctgt acatgcttga gggcgactgc caccggcgtg tcgcctgcga
      841 aaggcagcct gccggtgagc atctcataca tcactacgcc cagcgaataa atgtcggact
      901 ccgcggtggc cggagcgccg cgtgcctgtt caggcgaggt gtagttcacg gttccgatga
      961 ctctgccagt ctcggtgaga gcgctcgccg acaccgcccg cgctatgccg aagtcagtca
     1021 cctttacgcg tccgtcgcgc gttatcaaga tgttgtgcgg ctttatgtcg cggtggataa
     1081 ctccgttggc atgtgcatga tccagagctt ccagtatctg cgcgctgtag tcggcggttc
     1141 ggtccacgct gagtcgaccc tcgctgacta tgatatcgtt gagtgtgcgg ccgtccacac
     1201 actccataac aatgtagttg gacccgtctt ccctgcccac atcgtacaca ttgacgatgt
     1261 tggggtgaga cagagccgcg gcgctctggg cctcccgcct gaacctttcg cgaaactcgg
     1321 cgtccgatgc atactgcggc ctgagcacct ttatggcgac tgttcgctgc agaaatccgt
     1381 ccctggcacg gtagactgtg gccattccgc cttcgcctat tgtatcaagt atagagtacc
     1441 tgccacccag gagttcgccg gtcactccct cacctcccgc tcaacactgc tctgaagatg
     1501 ctgccggcta gaggtgctgc tacctgcccg ccggagcccc cgttctcgat tatgatggac
     1561 aagaccactt tcctccccgc atgctgggcg tagcctacga accatgcgtg cggcgctcca
     1621 tgggggttct ccgccgtgcc cgtcttgccg cccactacgt actctggaag attcgccgcc
     1681 ctggccgtgc cccattctac tgcagccctc attccgcctg ccacaatgcg ggccgcctcg
     1741 gccgacatgg gcacacacag cacaccaggg ctggagcgct tcaccaccgc cccgctcgcg
     1801 gagcggatct ccgtgacgag ccggggccgc atcattactc cgtgattgac aaccgcagcc
     1861 gccatcatcg ccacgtggag cggcgtcacc accagatcac cctggccgat ggcaagctgg
     1921 cccagaacgt catcgccggc ttcgctcgag aacgcggccg cggctgttgg cacatcaagc
     1981 ggcagggatc tgcataagta aaacccggcg agggcttccg gaagacgagc ccctgcttcc
     2041 agcgataaat ggacgaaagc cgtgttacac gagagcgcaa tggccgtctg gagatctatg
     2101 ctaccatgcg cgcccgcggc atcgctgatg atggtcccgc ctgcgcggat cgaacctcgg
     2161 caatcgtaca ctgttccact gcccgccaaa cccatatcga tggctgcccc tgctatcgcg
     2221 ggctttatag ttgaacccgg cggatacagc cctgcgagcg gcctgcatat cagcggggag
     2281 tcctcccggg cgatcacagt atcccatgcc gactcgagtg ctgcctgatg gacccatggg
     2341 gaactggccg cggccagcac ctcgccgctc gcagcatcca ccaccaccac ggccccacgc
     2401 cgccctgcga gcgctgcctc gcaggcgcgt tgagtgcgca agtcgatggt aagccgcacc
     2461 gagtagcccc tgtctggctg ctgagaaatg gtcgaaagca agccggtacg gctgtaagac
     2521 caacctcttc ctgcaagcca ttggtccatc gccgattcca ggcccgatct gccgagtgtc
     2581 gggtgcgcgt accccacaag cggcgccaga gagcccggcc cgcggtacac ccgtgactcc
     2641 agcccgcgct caagcgactc ggccaaaatc tctccgttgc gatccacaat gcttccacgc
     2701 cagatgccct tctccacgtt ggcaacgcgc ggattggccg ggcttgccgc gagcctatcg
     2761 gccgtgacgg cctggtagta ggtcgcggcc aaagctaata cggcgcatcc caccatccac
     2821 gccccaagca acctgcttat cctgtgattc acgaaccgtc accgccgctg ccgtccacgc
     2881 cgtcacggcc tccggctacc gcacttgcct tggcagacac tgaccgacac tcagctgtag
     2941 aacccagaag gatgcctgcc agggccaggc ttgcgatcat cgaactcccg ccgtagctgg
     3001 caaacggcag tgtaacccct gtcaggggca atacgcccag gtttccaccg attatcacga
     3061 aggtctgtac cgccaggatg ttggcggctc ctactgcggc caaccgctga cggggcgact
     3121 gggcacgcat ggcgcaggca tacgcgtggc cgactaagac gaagtatatg gcgataaccc
     3181 ccagcgaccc cgccagccct atctcctcgg cggacgctgc gaacaacatg tcggtcggcg
     3241 ccgccggtat cagccatgga gagccggagc cgtagccgcg gccgagcatg cctccgctgc
     3301 cgagtgcata gaatgcctgc acgatctgat aagatgaccc ggcaacatcc gcccagggat
     3361 tcgaccacgc cgcaaacctc gctcccacgt gcgaaaagaa cttgcaggcc agaccgacgc
     3421 ctgccactcc cagcgccgcc gcgccaagca caacatgtcg gttgcctgaa gcaacgtagg
     3481 caagggcgac gcacgatata tagatgattg ccgctgtgcc cacatcgcgc tgaagcactg
     3541 tgagggcgac aatcacaaac caaatcacga agaccatccg caccggcctt gatgcccaca
     3601 tctggcctag gccggcacga tgcggcgctc ctgacggcga cggcgactgc gacggcgcct
     3661 ctctacatgt gggcatcgac gacagtgcgt ccgcccccgc cagcacgcat gcgatctttg
     3721 caaactccga aggctgaaac cccatgccgc caatgaatat ccacgaccta gctcctccac
     3781 gctcctgccc aaaggccagc gtgaccagga gcagcgctgc agctccaacc gatagcgcca
     3841 ttccggagcg cgaagccctg cccttacatt gccagagcgc cgcgacaacc atcaccccga
     3901 cgccgatcca tatgaacgac agctgacgga gggccaagtc gggccggagc ctcaacaccg
     3961 aggcgtaccc aaaagccgcg gccgctgcaa ccaacgccgg aataacttga tcgccatcga
     4021 atcgcgtcat ggcaagcaac aggtgagcca cagcgaaagc cgcgcctgcc gcgaaacaga
     4081 gtacagcgtc ccatccgaac acgctgcccg atctggcgag cgaaagcgat gccccggaaa
     4141 gcccggcaag cagcatgacg atgcccacca gagaacgctc ggcgtcgcga cggcagggcg
     4201 accgaccgcc tgtgatcatg gctcactgcc tcccggagct tcctccggct ccgggctagc
     4261 aggccccggc atgtccgatt ccgcaacaac gcgtgccgcg atggtgatgt tgtcgtgccc
     4321 accgcgcgaa cgagccagcg acaccagatc atggcatgcg cttgacggat cgcgtgaccc
     4381 gcggaacgct gcagcaatct cctccgggca tacaaggtca tgaaggccat cggtacataa
     4441 gaccacaata tcccccggac gaacgggcca ctcgcccaca tccacctcga gatcgtcgaa
     4501 gaaacccagt gccctggtaa tggcgtgccg cttgggatgc gcggcggctt cctctgcggt
     4561 aattagcccc gagcgaagca tctcgcccac gacggaatgg tcatccgtaa gctgctcaat
     4621 cccgtctgcg gcaatcaggt acgctctgga gtcgcccaca tgccccactt tcagcactcc
     4681 gctcgtcaca gtcgcaaccg tcagggtagt gcccatgccc tccagtgcgt cggcatgcct
     4741 tgatgcgtcc agaacagtgg cattagcatg cttgatgcag cgctcaatat cgaagccggt
     4801 gggtccactc cgtgacagct cctgctccac agcgagtatg gccatacggg aagcaacttc
     4861 gccggccctg tgacccccca tgccgtcagc cacggcacag cagttctccc cgacgaagca
     4921 ggcatcttcg ttgtgctccc gaaccagccc cacgtctgtc gcgcatacac acttcatggc
     4981 cctcacccca tccacgacca cttctagccg tcactatcct cttctgcttt ttcgtctcct
     5041 tcgcggcagg caaccgacga ggcctgcgca tcggagatga ggcatgcgca tacccgatac
     5101 agaaaccagt agagcacagc tagacatgcc agcctgaaca tgactaatgc cgctcggatc
     5161 accccgggtc ggcctccttc gcgctgcgcc gacgcgccca cctgcgccta agctgcccac
     5221 aggcagcatc gatgcccgac cccatctccc gcctgactgt gacctcgata ccgcgcgact
     5281 cgagcgcgtc ggcgaaggcc tgtaccacct gaggcgtagg acgagcatat gtggtctcca
     5341 gcaccgggtt gagtggtatc agattcacat ggcacaaggt gccgcgaagc agacccgcca
     5401 gtcgctcagc gtgagcctgc gaatcattca ccccggctat cagcgcgtac tcgtatgtaa
     5461 cgcgcctacc agtaatctgt gcgtaccggt tgcacgcctt caccagagta cttatgggat
     5521 aagccgaatt caccttcatg atcgatgacc gagtcgcgtc atccggagca tgcagcgaca
     5581 ccgacagagt caacggaatg ccctcgccgg caaggcgcag aatgcctggg actatcccgc
     5641 aggtggaaat cgtgatgtgc ctgtacccga tggcgaaacc ccgatcccag ttggcgagcc
     5701 tgacaaactt gatgacatta tcgtagttga gcagcggttc gccgctgccc atcaccacca
     5761 gcgagctgat tcgtgcgcca tggaaacgct ctgccgcatc gctgcttgcg gcgagcagtt
     5821 ggtcgattat ctcgccgcat gtcaggtcgc gcactaagcc tccgaggccc gacgcgcaga
     5881 acgcgcaacc catagggcat cctacctgtg atgaaacgca gaggctcagc ccgtaatcgt
     5941 gccgcatgag cacggcctcc acggcgttat cgtcagccag ccccatcagg tacttgacag
     6001 tgtcgccctc cggcgaaacg cgtcgggcca gtatctcgag gggcccgaaa ggtgcgacct
     6061 cggccagctt gacccggagc agcctgggaa tgttggtcat gtcgccatac gacgaaactt
     6121 ggccatggta aagccattcg aggacctggt cggccctgta tccaggctcg cctatggact
     6181 cgaagaagtt cctacactcc tcacgcgaca tgctcttaat gtccacagtg ctaatcattc
     6241 caccaaatca caagaattcc tctgcttcag tctatcaccg aatgggcggc ccgtgcgcgg
     6301 ggccgcccta cacgtatttg atgcgcactg acgcaagcgc ctcttgctat gctcctccgc
     6361 gtctcacgta cggcctgccg ccggcggccg gaggcgtaga ccggcctata acgcccgcaa
     6421 gcgccgccat ggtgacgata tacgggaaca tcagcaggaa ctcctggggt atcgcccgaa
     6481 atccccacgt ctgcgcggta agctgcactg cgtcggccag cgcgaaaagc agggccgccc
     6541 acatagcgcc aagcggagtc cacttgccga atatcacggc agccagggcg atgaaccctc
     6601 gccctgaact catcccttct cggaagaacg ataattgccc aatagagagc gtggaacctg
     6661 ccatgcctgc gaggaaaccg ctgattatca cacacaggta acggataccg ataacgttga
     6721 cgcccacggt gtcggctgcc tccgggtgct ctccgcaagc acggatacga aggccaagag
     6781 gagtcttaaa cagcacgata tgccccacta tcaccgcaat gaacgccaga tatacagggg
     6841 gagtattcct cccaagcacc tgcccgatca cgggcacgtc gcggagaaac gggatgaccc
     6901 agttggatag cttagccacg cgttcagtct gacccgcccg gttgaatatc gccctgagca
     6961 ggaaacctgt gagcgccgtc gccagcacat tgatggccac tccggacacg gtctgattgg
     7021 ccctgtagcg tatcgaaacc acagcatgga tggtggctat cagcgcgccg gccacggccc
     7081 ccatccaaaa tccgagccac ggattacctg tcatgtacga gaaaaggact gccacaaagg
     7141 atcccgacag catcatgcct tcaaggccga tgttgaacac tccggccctc tcgctgtaca
     7201 ccccgccgat ggcagtgaga atcaggggcg cagctaggta cacagttgac gccaggaggt
     7261 ttatgacaac gagcttgatc atgttgaaat ccatggtgat caggcctcct ccttccctgc
     7321 agcggttgcc ctgcgggcac gggccctgat gcccgggatg cgaagccagc cccgaacgag
     7381 cccttcagcc gcaaccagca ggatgatcgc agcctgcact atgcctgcta cttccttggg
     7441 cacatgcgcc atgccctgca tcatctgcga accgcgcgac agcgtaccga agagggtcgc
     7501 agcgccaatg accccgaagg ggttcccttt gccgacaagc gccacggcaa tgccgtcaaa
     7561 gccgaatcct tcgaacacaa acaggtcaag gaacttatac tgtatggcgc acacctgcac
     7621 ggctcctgcc aggcccgaca tagcgccggc aagcagcatc gatatggcca tcgcgcccga
     7681 gacgttcatg cccgcatagc gcgccgcgtc cgggttcgcg cccacggccc ggatttcata
     7741 gcccgtcgtc gtcttgtaca ggatgtagaa aacgagcaca gcagcgccga tcgctatgag
     7801 tattcctgtg ttgacgcgga actcgctgtg gaagtcgccg aagaagcgca taagcttggc
     7861 cgaatcggca atctccgcac tgtagggggc agcgatattc ggatctttga ttggcccgtt
     7921 gaccaggtag tgcgagagga acagagcgat gtagttcatc attatgctat tgactacctc
     7981 atgcactccc agcttggcct tgaggtatcc tggtaccgcc gcccatatcg cccctgcggc
     8041 ggcacccgaa agcaccgtta gcggcagatg gataatgcgc ggcaaccccg atccgctgac
     8101 gccgacgacg gccgcggcca gcatgcccac gattatctgg ccttcagccc caatgttgag
     8161 tagacccgct ctgtaggcaa cggcaatcga tagccctgta aagataagcg gcgtggtgta
     8221 cacaatcgtc tcggagatgt tcacaggcga tccaaaacag cccttaagca gtgcctcgta
     8281 ggccgtca
//
LOCUS       DAOTAC010000087         8284 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_7345_length_8284_cov_4.100498, whole genome shotgun sequence.
ACCESSION   DAOTAC010000087 DAOTAC010000000
VERSION     DAOTAC010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 8284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8284
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7345_length_8284_cov_4.100498"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="PLM74_05095"
     CDS             <1..181
                     /locus_tag="PLM74_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HON42496.1"
                     /translation="VKYAIDRIVWLHGHRDLIGGLTFTEEPAVLRFFFGRLKPTSGWQ
                     AELARAFRADFGDSL"
     gene            complement(352..891)
                     /gene="prxU"
                     /locus_tag="PLM74_05100"
                     /pseudo
     CDS             complement(352..891)
                     /gene="prxU"
                     /locus_tag="PLM74_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_084691462.1"
                     /note="Most members of this family contain a
                     selenocysteine.; internal stop; incomplete; partial in the
                     middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent peroxiredoxin"
     gene            1027..1206
                     /locus_tag="PLM74_05105"
     CDS             1027..1206
                     /locus_tag="PLM74_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010632137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HON42497.1"
                     /translation="MDSYQVVLDFFTKSDKPVKAGEVAAATGLDKKEVDKAMAKLKKE
                     GKIASPKACYWEIGK"
     gene            1305..2597
                     /locus_tag="PLM74_05110"
     CDS             1305..2597
                     /locus_tag="PLM74_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42498.1"
                     /translation="MKFRTHCLIGVIVVLILAASAAHAASVLWTKYVSADGSFSFHHP
                     EGWHITESESVVVVDGPSTDEQICMCILPYYRNWSPEEHAQLMLSALQSEYPDLAASG
                     WEADSEGRAVLFDLTYGDGAQQYRGTGLVLKDSGAEQALWFHYIAPSSGYSRDRGAST
                     LKGFIGSIASGSASQSPVLGSGGRIAENADAFMFVLEFALGSPLSLSEERMIRDELER
                     GWSERSDAELAAYDDYPQMVSLIMAIGDQRQLMEVQHALAEAVLEWIGGSDPSDPVIQ
                     MIHAHVLEGDRALVGGDPPLTETAAASYAELMAYAELLQADPEAAPGSISHSTVEEIK
                     RQLAQCWLQLGRDERELVRTIPAVWTTLRKALSHGDSSDCERARSIIAELAPGRTSHS
                     AQGRVMSMVEHNLMMWAQQNTFNHWMWCQGFRYTPFGY"
     gene            2844..4142
                     /locus_tag="PLM74_05115"
     CDS             2844..4142
                     /locus_tag="PLM74_05115"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HON42499.1"
                     /translation="MEIHTDRRQGVMEKLRGAMSLFTPALLVFMVGMILANIAGAMNG
                     SLMPLYVQSLGADTKQIGLFFTISAIAPLVLQIIGGFVSDSIGRLKAVAIGSIAGSIG
                     YVLYAIAPAWGWLLVGHAVSRLAGCFVAPSFQAFIAEQSTDETRARVFAVSESAFAVV
                     GIVGPPIGGFLSQRYGFRLMFIAAGALYLVATVIRMAMALASRNQSSSTYHAPQKMTL
                     SALKSNMGSMAALVLAGGVMTWLFLSDSVNDIAFSVANHFDPIYQSNVIGMSNIQITW
                     VISAFSIVRMIALPFGGVFADKAGERTGICLGHLLCGLGGAVFLVGTAHLHFVLVSAL
                     YGLGVAFLSPAYNSLISKEVPIGIRGTAFGLVSTTLGIASLPAPYIGGLMWARFGPKA
                     PFVLPLLASMSMAAILWFKLPGKRDKAEQTAEAQVSPSQA"
     gene            4675..5898
                     /locus_tag="PLM74_05120"
     CDS             4675..5898
                     /locus_tag="PLM74_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011369324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HON42500.1"
                     /translation="MVMFLIGAMTVSAAVGGRLEIFSWWAGDEGPALEALIKLYEKSN
                     PGTKVINSTVTGGSGVNAKAVLKTRMLGGDPPDTFQVHAGQELIGTWVVAERMEDLTS
                     LYEKEGWRRVFPEGLLKLLSTDRGIWSVPVNIHRSNVMWYVPGNLAKWGVKAPTTWAE
                     FLAIAPALSAKGIVPLSLGENWTANHLWESVALAVLGPEKWDALWSGKLSFRSPEAVE
                     VWKTFGEILKYTNADAPSLSWQQATDMVVRGQAAFNIMGDWAAGYMATTLKLKPGTDF
                     GWMASPGTNGMFMMLSDSFGLPVGCAARDNALAWLKMLGSKEGQDTFNPLKGSISART
                     DSDMSKYNTYLQSAAKDWKSNVIAGSLAHGVVANERFMNDFATVMEIFLQSHNAQAAA
                     NAAQAVAIQSGIASR"
     gene            6000..7115
                     /locus_tag="PLM74_05125"
     CDS             6000..7115
                     /locus_tag="PLM74_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019551144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HON42501.1"
                     /translation="MHSRRDELIAILVILPSIVLLAIFVYAFIGQSIKASVTDWGQQA
                     ALSLNPEIHYVGMQNYKDLFTGFLGMRFRQDLTSMICFTALFVVVALAVGLGLATLID
                     HLVWGEAVFRTIFLFPMSLSFVVTGTIWRWLLQPRGGINQLPTLVGLPAGQFLWLSSR
                     EQRFAFDWAALPHYACWMLAIILAVLAWTNSRRAKPKTAGRQAVGAAALLVLAISGIT
                     RRLAILDFPEIHGFNVAIWGVVIAAVWQQSGYTMALYLAGLRTIPEELREAARVDGAS
                     RFQIYRYVELPMLTPITVSAVVILGHIALKIFDLIFAMAGPDHAPTSVPAITMFLKTF
                     RGNEFAVGSAIGVVLLVLVSLLIIPYVATSFREGGDQ"
     gene            7115..7939
                     /locus_tag="PLM74_05130"
     CDS             7115..7939
                     /locus_tag="PLM74_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017368160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HON42502.1"
                     /translation="MPAKGATRVFVQALAVLLALVYLIPVYMTIVTSLKSPEEISHLK
                     AWSMPASGANWSSYSTAFSKFAPYLKNSFVLATAATLLSASMGAINGYVMSKYPFPGS
                     RVVMPLIVFGMFIPYQSILVPLFQFLQKTRLYGGLPGLIVVHVVYGLPITTLLFRSFY
                     AEIPDELLGAARIDGAGFAGIFRYLILPLSGPPFLVVGIWQFTQIWNEFLFAVTLARP
                     DAQPITVALANLAGGQAVSWNLPMAGSVLAALPTVAIYVLLGRYFVRGLLAGALKG"
     gene            complement(7998..>8284)
                     /locus_tag="PLM74_05135"
     CDS             complement(7998..>8284)
                     /locus_tag="PLM74_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019678971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HON42503.1"
                     /translation="LRSVHMGQSVFGDAIVTKLPTLLRGDEQVDLAGRGITDKERGII
                     ELVAKGLSNREIAETLYLSEGTVRNYLSVILEKLELRDRTQLAVFYLSRS"
BASE COUNT         1642 a         2281 c         2542 g         1819 t
ORIGIN      
        1 ggtcaaatac gccatcgatc gcatcgtgtg gttgcacggc caccgcgacc tgatcggagg
       61 gttaacattc actgaggagc ctgccgttct gcgtttcttc tttggaaggc tgaagcctac
      121 gtcaggctgg caggctgagc ttgcacgagc gttccgcgcg gacttcggcg acagtctata
      181 ggcgctcgcc gccttctgcg cagaaccgag agggagacag acgtagtgcg gctaggtcgg
      241 gcaattgagt agggggctcc gtatgcggag ccccctgatc aatatcaacc tcaaccccga
      301 ggtagactac tcgtcgaatg cctcgctgac tttccatacc ttccatacgt ttcacactag
      361 gtcagggttg ggcttgagag tcttcttacc aggtttccag cccgaaggca tggcttgagt
      421 gccttttgag gcgcgtacgt gctggaaagc tctgatctgc ctgatggttt cgctaacgtt
      481 tctgcccaca ggaggcgtga gaacctcgaa tccctgaaca ttgccgtctg gatcgatgat
      541 gaagcgccct ctggtctcga cgccactgtc ttcgtcatat actccgtaca ttctgccgat
      601 gctgcctccc ggatccgaaa gcatcgcaaa ggggatgtcc tcgccgatca tcttcgacag
      661 ctcgtaatcg ttccacatct tgtgcacgaa tacactgtct acactcatgg atagaacttc
      721 aacgtccagg ctttaaagct cttcgtgctt ctcagcaact gctgagacct cggtcgctca
      781 gacaaaggtg aagtctccgg ggtagaagca caaaagcacc cacttgcctt tgtactccga
      841 aaggcctgtg ttcacgaact tgcccttgta gaaagccggg gcagcgaatt ccttgccaga
      901 gggcgtaata ccctggcact gaggaggagt tgcagcggca tagtcgaaat ccgaaacgca
      961 agagcctctt agctcttagt ctctcgtggc gaaaaagctc tgaggccgcg aaaggaggac
     1021 atatcaatgg atagctacca ggtcgttctt gacttcttca cgaaatcgga taagccagtg
     1081 aaggcgggcg aagttgccgc ggcaacgggc ctcgacaaga aggaagtgga caaggccatg
     1141 gccaagctca agaaggaagg aaagatagca tctccgaaag cgtgttactg ggagataggc
     1201 aagtaggttc ggcctgcccg cggacagctt gtgcgccgac atcaggggcc atggaagcga
     1261 tggccataag ggaaccccag ccacaaaaag gggagtgagc gaccgtgaaa ttccgcacgc
     1321 attgcctgat cggcgtcatt gtcgtcctga tactagcggc gtctgctgcg cacgcggctt
     1381 ctgtactatg gacgaaatac gtgtccgccg atgggtcgtt ttcatttcat cacccggaag
     1441 gctggcacat caccgagagc gagtcggttg tagtagtgga tgggccgagc accgatgaac
     1501 agatatgcat gtgtatcctt ccttactacc gcaactggag ccccgaggag catgctcagc
     1561 tcatgctctc tgcgctgcag tcggaatacc ccgatcttgc ggcatcggga tgggaggcgg
     1621 actctgaagg ccgagcagtc ttgttcgacc tgacgtacgg ggacggagcc cagcagtaca
     1681 gggggacggg cttggtactc aaggatagtg gggcagagca ggccttgtgg tttcactaca
     1741 tcgccccatc gtcaggctac tcgcgcgacc ggggcgcctc cactctgaag ggcttcatcg
     1801 gctcgattgc gtcaggatcg gcatcgcagt cgcctgttct cgggagcggc ggacgaattg
     1861 ccgagaatgc ggacgcgttc atgttcgtcc tcgaattcgc tcttgggtct ccgctatcgc
     1921 tatcggagga acgcatgatc cgcgacgaac tggagagggg ctggtcagag cggtccgacg
     1981 ctgagctggc ggcgtacgac gactatcctc agatggtctc cctgatcatg gcgataggcg
     2041 atcagcgcca gctcatggaa gtgcaacatg cattggccga ggcggttctt gagtggatag
     2101 gtgggagcga cccgagtgat cctgtgatcc agatgataca cgcccacgtg ctggagggcg
     2161 acagagcttt ggtaggcggc gatccccctc tgacagagac tgcggctgca tcgtatgccg
     2221 agcttatggc ctacgctgag ctgctacagg cggaccccga ggccgccccc ggctcaatct
     2281 ctcacagcac cgtggaagag atcaagagac aactggccca gtgctggctg cagcttggtc
     2341 gcgacgaaag ggagctggtc cggacgatcc ctgcggtctg gactactctg cggaaagcgt
     2401 tgagccatgg cgacagctcc gactgcgaga gggcgaggag catcatcgcc gagctggccc
     2461 cgggaagaac gtcgcacagc gcccaaggcc gcgtaatgag catggtcgag cacaatctca
     2521 tgatgtgggc acagcaaaac acctttaacc actggatgtg gtgccagggc tttcggtaca
     2581 cacctttcgg gtattaaccg tctatcgcct ggaggtgctg acacatgcga tttccgatgc
     2641 cgtgcgcaag atagcccatt cgaacctctg ccatatcaca ctggccattc ggctagaccc
     2701 cgtcccgccc tcttggccgt gggaatcggg cgcgtgatgg cctatcatat ccagtgtgga
     2761 ttcctctgct cgatggcgtg caaggagttg cgcgcgaagt agcacggaga gtcgtgcaga
     2821 gaggaacagg aagaggagcg ggaatggaga tacatactga ccgaaggcaa ggcgtcatgg
     2881 agaagctgag gggggcgatg agcctgttta ccccggctct cttggtgttc atggtgggca
     2941 tgatcctggc gaacatagcg ggcgcgatga acggttcgct aatgccgctt tatgttcagt
     3001 cgctgggcgc cgacaccaaa cagatcggct tgttcttcac gatctctgcc atcgcgcctt
     3061 tggttttgca gataatagga ggcttcgtat ccgattccat tggccgcttg aaggctgtag
     3121 ctatcggaag cattgcgggc agtatcggat acgttctcta tgctattgcg ccggcgtggg
     3181 gctggctgct cgtgggccat gcggtgtcgc gattggccgg ctgctttgtg gccccgagtt
     3241 tccaggcgtt catcgcggag cagtccaccg atgaaacgcg ggcgcgggtg tttgcggtca
     3301 gtgagtcggc attcgctgtg gtaggaattg tgggtcctcc cataggcgga ttcctatcac
     3361 agcggtacgg gtttcgcctg atgttcattg cggcgggagc gctgtatctc gtggccacgg
     3421 tcattcgaat ggcaatggcg ctcgcatcgc gcaatcagtc atccagcacg taccacgcgc
     3481 cgcagaagat gacgctatcg gccttgaagt ccaacatggg ctccatggcg gcgctggtct
     3541 tggcgggcgg ggtcatgacc tggctgtttc tgtcggatag cgtcaacgac atcgctttca
     3601 gcgtggcgaa ccatttcgac cccatctacc agtcgaacgt tattggaatg agcaacatac
     3661 agatcacgtg ggtgatctcg gcgttctcta ttgtgaggat gattgcgctg cctttcggcg
     3721 gtgttttcgc cgacaaggcg ggtgagagga cggggatatg cctgggccat cttctgtgcg
     3781 ggctgggtgg ggctgtattt ctggtgggga ctgctcacct ccatttcgtg ctggtatcgg
     3841 cattgtacgg gctgggagtg gcgttcctat cgcctgccta caactcactg atatccaagg
     3901 aggttcccat aggcatcagg ggaactgcct tcggactggt gtcgaccacc ctgggcatag
     3961 catcgcttcc tgcgccgtat atcggcggct tgatgtgggc cagattcggg ccgaaggctc
     4021 cgttcgtgct gccccttctg gcaagcatga gcatggctgc gatactctgg ttcaaactgc
     4081 cggggaagcg cgacaaggca gaacagaccg cagaggcgca ggtgtcgcca tcgcaagcat
     4141 agccgacgta tagctgtgta gttgtgttga ttcgcgcctg gaggataggc cggggcagtt
     4201 gggccccggc cttctttcga ctgttgttgt ccaccgaaat ctcgcctatt tctgctccta
     4261 atccacatgc gggctaccga gaggcacgaa atgtgtgtgc aggagtactt gcctgtgtgc
     4321 cgaatagcaa ttgtagccac ttactggcca tgttccgaaa acggtgaaaa cgcgcacccg
     4381 aatcgcattt gccgtgccac cttatctaat gatcatctac cactctccct ccttacatca
     4441 cagagacgaa ggtctagtgc ccgaccgggt gcggtccctg atcgcatgct gcgcatgaaa
     4501 cgtatatgga ggtgatggct ccgagcgcgc cgagattcgc gtcgtctccg tgatgaccag
     4561 cgagatgttt gacgatcgag agagcaagac tcgcggagta cctgtcaacc agttctttag
     4621 ggagggatcc agatgaagag ttcgagatgg ctgaggctcg tggccctgag cagcatggtc
     4681 atgtttctca taggggccat gactgtgagt gcggccgtcg gcggcaggct ggagatattc
     4741 tcctggtggg ccggcgacga agggccggcg ctcgaggcgt tgattaagct gtatgagaag
     4801 agcaatccgg gaacgaaagt cataaactct accgtaaccg gcggatctgg agtcaacgcc
     4861 aaagcagtcc ttaagaccag gatgctgggc ggcgatcctc cggatacatt ccaagtgcat
     4921 gccggccagg agctgatcgg aacgtgggtt gtggccgaga ggatggagga tctgacctcg
     4981 ctgtatgaaa aagagggctg gagacgggtc ttccctgagg gcctcctcaa gcttctcagc
     5041 actgaccggg gtatatggtc ggtgcctgtc aacatccatc gctccaacgt catgtggtat
     5101 gtgcctggca acctcgccaa gtggggtgtg aaggcgccga ccacgtgggc ggagttcctg
     5161 gcgatagctc ctgcgctgtc ggctaaggga atcgtgcctt tgtcactcgg cgagaactgg
     5221 accgccaacc acctgtggga aagcgtggcc ctggcggttc tgggccccga gaagtgggac
     5281 gccctatgga gcggcaaact gtcgtttagg agccctgaag ctgtcgaggt gtggaagacg
     5341 ttcggtgaga tccttaagta tacgaacgcc gacgcgccgt cgttgtcctg gcagcaggca
     5401 accgatatgg tagtcagggg acaggcggcg ttcaacatca tgggcgactg ggctgcgggc
     5461 tacatggcta ccaccctcaa gctcaagcca ggcaccgatt tcggctggat ggcatcgccg
     5521 ggtactaacg gaatgttcat gatgctgtca gactccttcg ggctcccggt gggctgtgca
     5581 gccagagata atgcgctggc gtggctcaag atgctgggca gtaaggaagg acaggacacc
     5641 ttcaatccgc tgaagggatc catcagcgct cgcaccgact ctgacatgag caagtacaac
     5701 acgtacctgc agtcggctgc gaaggactgg aagtccaacg tgatcgccgg ttcgctggcc
     5761 cacggtgtgg tcgcaaatga gaggttcatg aacgacttcg cgacggtcat ggagatcttc
     5821 cttcagagcc acaacgcgca ggccgcggct aacgcggctc aagctgtagc gattcagtcc
     5881 ggcattgcgt ctaggtagtg gaccggtcag gggctggata ggctggatag gccccacaga
     5941 tagatagacg gttagggggc ggtcggggcg ccggccgccc cattgtgagg tggtaacgcg
     6001 tgcactccag gcgagatgaa ctcatagcca tactggtcat cctgccatcc attgtgttgc
     6061 tggcgatatt tgtgtatgcg ttcataggcc aaagcatcaa ggcctcggtc accgactggg
     6121 ggcagcaggc ggcgttatct cttaaccccg agatacacta cgtgggaatg cagaactata
     6181 aagacctgtt cacagggttc ttgggaatgc ggttccgtca ggacctgacc agcatgatct
     6241 gcttcactgc gttgtttgtg gtggttgcgc tggcagtcgg actcggcctc gccacgctaa
     6301 tcgatcacct tgtgtgggga gaggcagtct tcagaaccat cttccttttt cccatgtctc
     6361 tctcgtttgt ggtcaccggg acaatatggc gatggcttct ccagccccgc ggcgggatca
     6421 accagctgcc caccttggtc gggttgccgg ccggccagtt cctctggctt tcaagccgag
     6481 agcagcgttt cgcgttcgac tgggccgccc tgccacacta tgcttgctgg atgctggcga
     6541 tcatacttgc cgtgcttgcc tggaccaact cacgtcgcgc aaagccaaag accgccggcc
     6601 ggcaggcagt cggagccgcg gcccttctgg tgttggccat atcgggtatc acacggcggc
     6661 tggcgatcct tgactttccc gagattcatg ggttcaatgt ggcaatctgg ggagtagtga
     6721 tcgcagccgt gtggcagcag tcagggtata ccatggcact ctacctggcc gggctgcgaa
     6781 ccattcccga ggaactacgt gaagcggccc gcgtggacgg cgccagcagg tttcagatat
     6841 acaggtatgt ggagcttccc atgctcactc caataaccgt gagtgcagta gtcattttgg
     6901 gccacatcgc tctcaagata ttcgacctga ttttcgcgat ggcagggcct gatcacgccc
     6961 caaccagcgt gcccgcgatc actatgttcc tcaagacatt ccgaggcaac gaattcgccg
     7021 tgggctccgc cataggcgta gtcctgctcg tgctggtatc gctgctgata atcccttacg
     7081 ttgccacgtc ctttagggaa gggggagatc agtaatgccg gcgaaaggtg caacccgcgt
     7141 attcgtgcag gccctggcgg tcttgctggc cttggtatac ctgataccgg tatacatgac
     7201 catcgtgact agcctgaagt cgccggagga gatcagtcac cttaaggcgt ggtctatgcc
     7261 tgccagtggt gcgaattgga gcagttactc gactgcattt agcaaattcg caccatacct
     7321 caagaacagc tttgtgttgg ctactgcggc cacactgctg tccgcctcca tgggcgcgat
     7381 caatggttac gtgatgtcca agtatccttt ccccggatcg agagtggtga tgcccctgat
     7441 agtcttcggg atgttcatcc cgtatcagtc catactggta ccgctgttcc agttccttca
     7501 aaagacccgg ctgtatggag gactaccggg gctgatcgtg gttcacgtgg tgtatggctt
     7561 gcccataacc acactgctct tccgcagttt ctacgcagag atcccagacg aactgctcgg
     7621 tgcggcccga atagacgggg ccggatttgc gggcatattc cgctacctca tactccccct
     7681 gtctgggcct ccatttctgg tggtggggat atggcaattc acgcagatat ggaatgagtt
     7741 tctgttcgca gtcacactgg ccaggccgga cgcccagccc attaccgtgg ccctggcgaa
     7801 cctcgcaggc gggcaggctg tgtcgtggaa ccttcctatg gcgggctcag ttctcgctgc
     7861 gctgcccacg gtggccatat acgttctgct cggccgctac ttcgtccgag gcttgctggc
     7921 cggggctttg aaaggctaga tgagcctgac gacaatgatg atacgggcag ggcttctggg
     7981 cattgtggcc tccgggccta cgaccggctc agatagaaga ccgccaactg cgtgcggtcg
     8041 cgcagctcca gcttttcgag gatgacgctg aggtagttgc gcactgtgcc ttcgctgagg
     8101 tacagggttt ccgcgatctc gcggttgctc agccccttgg ccacgagttc gatgattcct
     8161 ctttccttat cggtgatgcc gcgcccggcg agatcgacct gctcgtcgcc gcgtagcagt
     8221 gtcggcagct tcgtgactat ggcgtccccg aacacgcttt ggcccatgtg tacactcctg
     8281 agcg
//
LOCUS       DAOTAC010000088         8233 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_7404_length_8233_cov_4.942284, whole genome shotgun sequence.
ACCESSION   DAOTAC010000088 DAOTAC010000000
VERSION     DAOTAC010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 8233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8233
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7404_length_8233_cov_4.942284"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="PLM74_05140"
     CDS             complement(<1..735)
                     /locus_tag="PLM74_05140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HON42504.1"
                     /translation="MREPVIDLEHLSADLRVVLDSINEGIHIVDTSGITVFYNKVAAD
                     LDNLNPAEVVGRHILSIFPSLSEDTSTLLSVLRTGRPAPVRQQTFRNFKGDTITTVNS
                     SLPIYSGARLVGAIEISRDVTLVKALAERVVDLQAQLLSESAGQGEQSHLPKAGTARY
                     TFSDVVAASQSMLHVVECAKRAAASSSSIIVAGETGTGKEVIVQSIHNSGSRSSRPFI
                     AQNCAALPDTLLEGILFGTAKGAFTGA"
     gene            complement(909..1841)
                     /locus_tag="PLM74_05145"
     CDS             complement(909..1841)
                     /locus_tag="PLM74_05145"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HON42505.1"
                     /translation="MTHKRMRVALLMGGSSSEREVSLKSGEQVEAALRRLGHEVITVD
                     PAVDSIAVLADSRPDAAFIALHGRYGEDGCIQGALEMLGIPYTGSRVLASAVAMDKIV
                     SKVLFNAAKVPTPPYVTVHRSDAPELGLTHAESIGFPCVVKPSSQGSTVGITIVKESS
                     ALADALELAFRRDPDALIEKYIAGTEVTVGVLGTRTPRALPTLEIVPKNEFYDWDSKY
                     TPGKSEHIVPARLPKPARAECERLAVEAHAALKCRGYSRVDMIVEPSGQVWVLEANTL
                     PGLTQVSLLPDAARAEGISFDQLIQTILDSAFDE"
     gene            complement(2113..2859)
                     /gene="surE"
                     /locus_tag="PLM74_05150"
     CDS             complement(2113..2859)
                     /gene="surE"
                     /locus_tag="PLM74_05150"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HON42506.1"
                     /translation="MSNDDGIAAPGLAALVESVCAIADEVWVCAPDRERSAISHALTM
                     RAPLRARRIEPRWPSTTAWAVDGSPADCVKLALEELMPERPHVVLAGVNRGPNLGTDI
                     LYSGTVAAAAEGALAGIPAVALSTVSYDPSDYGPASTVGAALARLAATRGLPPGVMLN
                     VNVPDGAELDRIVVTRMGVQRYSNIFERRVDPRGEVYYWMCGSPEASAPGDGLDTDAV
                     RSGAISITPIRFDMTDHVAYDILSSWDIRI"
     gene            complement(2893..3573)
                     /locus_tag="PLM74_05155"
     CDS             complement(2893..3573)
                     /locus_tag="PLM74_05155"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HON42507.1"
                     /translation="MSLFLRNMGLLVVATLLGAVIGLERERQGKSAGLRTHSLVCLGS
                     ALITMVGIHGFGPSGGGLRDPARVAAQIVSGIGFLGAGTIMHEGLTIKGLTTAASLWT
                     TAGIGLAAGAGLVWPAIGATVLVEFVLVFLNRLEERYFPHKDVVLRVTAIDRPGLLGE
                     IATIVGELRMNIVASDINLERGESMVRIEIVMGHNMAAVGSRATHADLLQRVLALSGV
                     LRAEVDNW"
     gene            complement(3600..4496)
                     /locus_tag="PLM74_05160"
     CDS             complement(3600..4496)
                     /locus_tag="PLM74_05160"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HON42508.1"
                     /translation="MYSTCDECGRIRALIEQLGSIESDLARVDPEHREAAVEQIANLK
                     GQLLDVLCDALSSGDGPMRRAAAYGLSKLQDVQAVPLLARALGDSDETVRGWAVEALA
                     KCSTSLAVEPLVRALGDESVYVYYSACNALRSIPVDDVRPALQRELLSPEPTVRKRAA
                     KLLGDMGESEAVPHLVSLTTDPDSGVRYEAVEALRKIADSRAVDALIGCLDDESHEVR
                     LGAVQALRWAGDLRAVEPLVAVCLENEELRLLAIQALREIAGDEAISPVVVALRESRE
                     LVRATAAKILGSSRRSHKIGVS"
     gene            complement(4580..5188)
                     /gene="plsY"
                     /locus_tag="PLM74_05165"
     CDS             complement(4580..5188)
                     /gene="plsY"
                     /locus_tag="PLM74_05165"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HON42509.1"
                     /translation="MKTVNLHPLLVVILSYLVGSIPFGVILGSRIAGLDVRKHGSGNI
                     GATNVLRTLGLGFAVCTFVLDTGKGAFAFWLAHLVGLSPAWTAATGVAAVVGHNWPVW
                     LRFRGGKGIATTFGFVLLAMPWVGIVVAAVWVGVIAACRYASVASMVAIASSPIAAFL
                     AGYPREYTVACLLLALSAVARHVSNIRRLLSGTENKFVLKLR"
     gene            complement(5209..6519)
                     /gene="der"
                     /locus_tag="PLM74_05170"
     CDS             complement(5209..6519)
                     /gene="der"
                     /locus_tag="PLM74_05170"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HON42510.1"
                     /translation="MARPLVAIVGRPNVGKSALFNRISRARVSIVDDTPGITRDRVYA
                     EAEWCGRAFTLIDTGGIEAYPGDSIAAQTRRQAEMAIEQADVVLFVVDARVGITSEDD
                     DVAGILRKSGKPVIVVASKVDDPSHMDYSLHSLGLGELIATSAVHGSGIGDLLDAVVQ
                     SLPDSEAEPEPDDDAIRVAVIGRPNVGKSSLVNRMLGEERSIVSDVPGTTRDAVDAVL
                     EFGGERYIIVDTAGIRRKGRVDVGIEKYSVLRAMNAIERCDVAVTVVDGTEQPSAQDT
                     RIAGYAHEAGKATVVAVNKWDIVERDDTTAREYERALRTYLDFMPYAPVVFVSALTGR
                     RIGKLLETVKSSAAQHRRRVQTAMVNRVVQDAVLRNPPPPDGGKEIRVYYATQVSARP
                     PRFAVWVSRPDSVHFSYRRYLENALREAFEFSGTPIEVLLKQRE"
     gene            complement(6550..7887)
                     /locus_tag="PLM74_05175"
     CDS             complement(6550..7887)
                     /locus_tag="PLM74_05175"
                     /inference="COORDINATES: protein motif:HMM:NF016354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HON42511.1"
                     /translation="MTVSAVIASVEPGSPAALAGITPGDTLIAINGHPIRDIIDYQYH
                     AASSSVRLSVGTPDGSEFCVVIDNDYDTRLGIHFDEIAFDGERRCANKCVFCFIDQLP
                     AGMRKSVYFKDDDYRLSFLQGNFVTLTNLTDSDIERIIHLRLSPLYVSVHSTDEETRR
                     ALLGRAGIATRPIMPTLKRLSEAGIEFHAQVVLVPGLNDGTMLEQTIEDLADLWPSCA
                     SLGIVPVGLTAHREGLHRLDAVDPHLAGQVIRVVHAAQRRSLDSRGTRFVFAADEFYY
                     LAQQEIPASDEYEEYPQLENGIGLSRLFSDEFEEALGRMAGQATAAVDFTAITGVLGA
                     RAIAHACASLSRIAGVCVHVLPVANRFFGESVTVSGLVVGADIVAALNEAKSSQTTDA
                     GFFGDFVAIPDVMLRRGQECFLDDMTPGDIERATGIPVRIVSASARGLLECIM"
     gene            complement(7960..>8233)
                     /locus_tag="PLM74_05180"
     CDS             complement(7960..>8233)
                     /locus_tag="PLM74_05180"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HON42512.1"
                     /translation="MVAEFEKAAFAMSPGQTSAPVKSEYGYHIIRVTERKDAHEATFD
                     EVKEDIERTIKGNQAKSAQALASELRLEADVTITDSKYKDLSTSAD"
BASE COUNT         1650 a         2613 c         2305 g         1665 t
ORIGIN      
        1 cgcgcctgtg aatgcccctt tggccgttcc aaacaggatt ccctccagca gggtatcggg
       61 aagcgccgcg cagttctgcg ctatgaatgg tcgactagat ctggatcccg aattgtgaat
      121 agactggaca atgacttcct tacccgttcc ggtctctccc gcgacaatga tcgaagagct
      181 gctggcagcg gcgcgttttg cgcactcgac tacgtgtagc atggactggc ttgcggccac
      241 aacatcgctg aaggtgtacc gggctgtgcc tgctttcggc aggtgcgact gctcgccctg
      301 acccgcgctc tcggaaagta gctgagcctg gaggtcgaca acccgctcag ccagggcctt
      361 tacgagcgtg acatcgcgcg agatctcaat agcccccaca agcctagcac cgctgtaaat
      421 cgggagcgat gaattcacgg tggtgatcgt gtcgcctttg aagttgcgga acgtctgctg
      481 gcgcacgggc gcaggccgac ctgtgcgcag tacggacagg agagtgctgg tatcctcgga
      541 gagggacggg aatatggaca ggatgtgacg accgacaact tctgccgggt tgaggttgtc
      601 tagatctgcg gccaccttgt tgtagaatac cgtgatgccg ctggtgtcaa ctatgtgtat
      661 gccttcattg atagagtcaa gaaccactct gaggtctgcg cttaggtgct cgaggtcgat
      721 cacgggttca cgcaccggca tttccctcct cagagacgct ctgtgcccaa gtatgggcac
      781 ccgccaagat tatatcgtac atggccgaca attgacagcc gggaccactc tgctttgaga
      841 gtgacctcgg ctgccggcgc ctatctggcg cctatccagc gcctatctag cgcctatcta
      901 gcgaacgtct actcgtcgaa tgcgctgtcc aggatggtct gtatgagctg atcgaaggaa
      961 ataccttctg cccgcgcagc gtcaggcagc aggctgacct gagtcagccc gggcagtgtg
     1021 ttcgcctcaa gcacccacac ctggccggat ggttccacta tcatgtcgac cctggagtaa
     1081 ccgcggcatt tcagagcggc atgggcttcc actgcaagcc tttcgcattc cgctcgcgcg
     1141 ggtttgggta gtcttgcagg cactatatgc tccgacttgc cgggagtgta tttggagtcc
     1201 cagtcgtaga attcgttctt tggtacgatt tccaaagtag gaagagcccg aggtgtgcgc
     1261 gtgcccagca ctcccaccgt gacttcagtg cctgcgatgt acttctcgat taatgcatct
     1321 gggtctcttc tgaaagccag ctcgagcgcg tctgctagcg ctgagctctc tttcactatc
     1381 gtgatgccca ctgtcgaccc ttgtgaagag ggcttcacaa cgcaggggaa cccgatgctc
     1441 tcggcgtgcg taagcccaag ctccggcgca tcgctgcgat gcactgtgac gtagggcgga
     1501 gtcggcacct tggccgcatt gaacaatacc ttcgaaacga tcttgtccat ggcgactgcg
     1561 cttgccagaa cgcgggaacc tgtgtatgga atccccagca tctccagagc tccctgaata
     1621 cagccatcct caccgtacct tccatggagc gctatgaagg ccgcgtcggg ccgagaatcg
     1681 gccaacactg cgatgctgtc tacggccgga tcgacggtga tcacttcgtg tccgagccgg
     1741 cgcagagcag cctcgacttg ctcacctgac ttgagcgaga cctcccgttc gctggaggaa
     1801 ccgcccataa gcagagccac tctcatcctt ttatgtgtca acgaattcac caccaacaca
     1861 tgcttcgata tcatctcggc cactgccgaa atacctcgct gaagacggcg actgtggcgc
     1921 ggcatgccaa aagcgcgtag actagcccgg caacgagcaa aaaccctcgg gccagggcgg
     1981 catgccgcca cgcgggatcg tcggccctca cggatttggc ccagaccacc gcagcaatga
     2041 tcgcaatcgc caacgccgac gataagacta agcagagccc cacgatgcat caccggtcta
     2101 tcgtatgcgg ggctatatgc gaatgtccca gctgctcagg atgtcgtacg ccacatggtc
     2161 agtcatgtcg aaccttatgg gcgttatgga gatagcgccc gaccgcaccg cgtcggtgtc
     2221 gaggccatcg ccgggtgccg acgcttccgg gctgccacac atccagtagt agacctcacc
     2281 ccggggatcg acccgtcgtt cgaagatgtt ggaataccgc tgaacaccca tcctggttac
     2341 gactatcctg tccaattcgg cgccgtcagg cacattcaca ttgagcatta ctcccggcgg
     2401 gagcccacgg gtcgccgcca ggcgcgccag agctgcgcct actgtggacg caggcccgta
     2461 gtcagaaggg tcgtaggaga ctgtcgatag tgcaacggcc ggtatcccgg ccagtgctcc
     2521 ttcagcggct gcggccaccg tccctgagta gaggatgtcg gttcccagat tggggccacg
     2581 gtttacgcct gccaagacca catgtggtcg ctcgggcatg agctcctcca gggcgagttt
     2641 gacgcagtcg gcaggacttc cgtccaccgc ccatgccgtc gtcgacggcc agcgcggctc
     2701 tatgcggcgg gctctgaggg gggctcgcat tgtcaacgca tggctgattg cgctgcgctc
     2761 gcgatccggc gcgcacaccc atacctcgtc tgcgatggcg cacacagatt cgactagagc
     2821 tgccagccca ggcgctgcta tgccgtcgtc gttggagatc agaattcgga tgataggtta
     2881 acccccttcg agttaccagt tgtcgacctc ggctctcaga acacccgaca gagccaaaac
     2941 cctctgcagc aagtcggcat gggttgcgcg gctccctacc gccgccatgt tgtgccccat
     3001 gacgatctct atcctaacca tggactcgcc gcgttccaag tttatgtcgc tggcaacgat
     3061 gttcatgcgc agttcgccga cgatggtcgc gatctcaccg agcagaccgg gcctatcgat
     3121 tgcagtgacc cgcagcacaa catctttgtg cgggaagtat cgctcctcca gccggttgag
     3181 gaagaccagg acgaactcaa ccagcacagt ggcgccgatg gccggccaga ctagacctgc
     3241 gccagccgcg agaccaatgc ctgccgtcgt ccaaagacta gccgcagtag tgagcccctt
     3301 gatagtgagg ccttcgtgca ttatggtgcc tgcgcccaaa aacccaatgc cgctcacaat
     3361 ctgagccgcc actctcgcag ggtcgcggag cccgccgccg gatggcccaa acccgtgtat
     3421 tccaaccatt gtgatcagcg cggatccgag gcagaccaga gagtgggtgc gcaggccggc
     3481 cgacttgccc tgcctttcgc gttcgagccc tatgactgcg cccagcaaag tagcgacgac
     3541 tagcagcccc atattgcgca aaaataagga catctctcat tacccctccg tggatttgtc
     3601 tagctgacgc cgatcttgtg cgagcgccgt gatgatccaa gtatcttggc cgcagtggcg
     3661 cgcaccagtt cacgagattc cctcaatgcg acgactaccg ggcttattgc ttcatctcct
     3721 gcgatctcgc gcagcgcctg tattgccagc agacgcagtt cctcgttttc caggcatact
     3781 gccacaagag gttcgactgc acgcaaatcg cccgcccacc gtagcgcctg gactgctccc
     3841 aggcgcactt cgtgcgactc gtcatcaaga cagcctatga gtgcatccac agcccggctg
     3901 tcggcgatct tcctcagcgc ctccactgct tcgtacctca cacccgagtc aggatcggtg
     3961 gtcaggctga ccaggtgagg caccgcctcg ctttcgccca tgtcgccaag gagcttcgcc
     4021 gcacgctttc tcaccgtggg ctcaggcgac agcagttcgc gctgaagggc aggccggacg
     4081 tcgtcaacag ggattgatct cagcgcattg caggcgctgt agtacacata tacgctttca
     4141 tcgcccaggg cacgcacgag cggctccacc gccagcgaag tgctgcattt agcgagagcc
     4201 tcaaccgccc atccccgcac ggtttcgtcg gaatcgccga gcgcccgagc gagcagcggt
     4261 accgcctgta catcttgcag cttggaaagg ccatatgcag cagcgcgccg catcggcccg
     4321 tcaccgctcg acaaggcgtc acaaagaaca tcgagcagct gccctttgag attggctatc
     4381 tgctcgactg ccgcctccct gtgttcggga tctacgcgag ccagatcgct ctcaatcgag
     4441 cccagctgtt caatgagagc gcgtatgcgc ccgcattcat cgcatgttga atacaaccag
     4501 cgcacccccg ggcaagtcga ctacttaggg gcaaattgca cagtaattcg acggaagtgc
     4561 agacgattcc tgctctcggc tatctgagct tcaggacgaa cttgttctcc gtgcccgaga
     4621 gcagacgccg gatgttggac acgtgcctcg ccacggcgct cagggcgagt agcagacatg
     4681 ctaccgtata ctcgcgaggg tatccggcga ggaatgccgc gattggcgag gacgcaatgg
     4741 ccaccattga ggcgaccgag gcgtagcggc acgcggctat gacaccgacc catactgctg
     4801 ctaccacgat acctacccag ggcatggcga gcagcacaaa cccgaatgtt gtggcgattc
     4861 ctttgccgcc tctgaagcgc agccataccg gccaattgtg cccgaccacc gcagcgactc
     4921 cggtcgccgc cgtccaggcc gggctgaggc ctactagatg cgcaagccag aatgcgaagg
     4981 cgccctttcc agtgtccagc acgaaggtac atacggcaaa tcctaggccc aatgttctca
     5041 ggacattggt agcaccaata ttgccgcttc cgtgcttccg gacgtctagc ccggcaatac
     5101 ggcttcccag gatcaccccg aacggaatcg aaccgaccag ataggacagg atcacaacca
     5161 gcaacggatg gagattcacc gtcttcacca gcctccgcca atgtcatgtc attccctctg
     5221 cttcagcagg acctcaatgg gtgtcccgga aaactcgaac gcctcgcgca aggcgttttc
     5281 gaggtagcgt ctgtacgaaa aatggacgct gtctgggcgg ctgacccaca ccgcaaaccg
     5341 gggcggtctc gccgacacct gtgtggcata gtacacccgg atctccttgc cgccatccgg
     5401 agggggcgga tttcgcagca ctgcatcctg cacgacccta ttgaccatgg cagtctgcac
     5461 tctccttctg tgctgggcag cgctggactt gacagtctcc agcagcttgc ctatgcgcct
     5521 ccctgtcaat gccgacacga agaccaccgg ggcatagggc atgaagtcca ggtaagtcct
     5581 aagagcccgc tcgtactctc tcgcagtcgt gtcgtctcgt tcgacaatat cccatttgtt
     5641 tacggccaca acagtggcct ttccggcctc atgggcgtat ccggctatcc ttgtgtcctg
     5701 agcggaaggt tgctcggttc cgtcaaccac ggttactgcc acatcgcacc tttcgatggc
     5761 gttcatcgct ctcaaaacgc tgtacttctc gatgcctaca tcgactcgac ccttccgtct
     5821 gatgccggct gtatctacta ttatgtatct ctcgccgccg aattcgagaa cagcatccac
     5881 agcgtccctg gtggtgccgg gcacatcgct cacgatcgaa cgctcttcgc cgagcatccg
     5941 gttgaccagc gatgactttc ccacgttggg cctgcctatg acggccaccc tgatcgcgtc
     6001 atcatccggc tcgggctcgg cctcgctatc aggtagcgat tgcacgacag cgtcgagcag
     6061 atcgccgatt ccgctgccgt ggactgccga cgtagcgatt agttcgccca gacccaagga
     6121 gtgcaggcta taatccatgt gggatggatc atcgaccttg ctggccacaa ctatcacagg
     6181 cttgcccgac ttgcgtagaa tgcctgccac atcgtcgtct tcgctcgtta tgcccacgcg
     6241 cgcgtctacg acgaagagca ccacgtcagc ctgctcgata gccatttcgg cttgtctgcg
     6301 agtttgagct gcaatactgt cgccggggta cgcttctatg cccccggtgt cgatcagggt
     6361 gaaggctcga ccacaccact cggcctcggc gtacacacga tcgcgtgtga tacccggcgt
     6421 gtcgtccact atagagactc tggcccgaga gatcctgttg aagagggctg acttgcccac
     6481 attgggccgc cccacgatcg ctaccagcgg tcttgccacc ttaccccacc tcccatccgg
     6541 taggtccgac tacatgatgc attcgagaag cccgcgggcg gacgcactga cgattctcac
     6601 aggtatcccc gtggcccgtt cgatgtcgcc cggggtcatg tcgtcgagaa agcattcctg
     6661 gccccgtctg agcatgacgt cgggaatggc tacgaaatcg ccgaagaacc cggcatccgt
     6721 cgtctgtgac gacttggcct cattcagcgc agcaactatg tctgctccta ccaccaggcc
     6781 ggacacggtg acgctctcgc cgaagaacct gttggctacc gggagaacat gaacgcaaac
     6841 gcccgcgatc ctgctcagac tggcgcaggc gtgggctatt gcccgggcgc cgagcacgcc
     6901 ggtgatcgcg gtgaagtcca ctgctgcggt cgcctgcccc gccatcctgc ccaaggcctc
     6961 ctcgaattcg tctgagaaca aacgcgacaa gccgatccca ttttcgagct gcggatattc
     7021 ttcatactcg tcactggccg ggatctcctg ctgcgcgaga tagtaaaact cgtcggcagc
     7081 gaacacaaac cgtgttcccc gcgagtcaag agagcggcgc tgagccgcat gcacaacgcg
     7141 tattacctgc ccggccaggt ggggatcgac ggcgtcgagc ctatgaaggc cttcccggtg
     7201 cgcggtgagc ccgacaggca ctatccccag cgacgcgcag gaaggccaga gatctgcaag
     7261 atcttcaata gtctgctcga gcatcgtccc gtcattcagg ccgggaacca acacgacttg
     7321 cgcgtggaac tcgatcccag cctcgctgag ccgcttgagc gtgggcataa tcggcctcgt
     7381 ggcgatgcct gctcgcccta gcaatgcgcg cctcgtctct tcgtcagtgc tgtgtaccga
     7441 aacatacaac ggcgacaggc ggaggtggat gattcgctcg atgtccgaat cagtgaggtt
     7501 agtgagcgtg acgaaattgc cctgcagaaa cgaaagccgg tagtcgtcgt ccttgaaata
     7561 cacgctcttg cgcattccgg ccggcaactg gtcgatgaag cagaaaacgc atttgttggc
     7621 gcagcgcctt tcgccgtcga atgcgatctc atcgaagtgg atgcccagtc gcgtatcgta
     7681 atcgttgtcg ataaccacgc agaactcaga gccatcaggc gttccaacac tcagacggac
     7741 actggaagat gcggcatggt actgatagtc gatgatgtcg cgtatcggat gcccgtttat
     7801 ggcaatcagc gtgtcgcctg gagtaatgcc ggccaacgca gcggggctac ccggctcgac
     7861 actggcgatc acagcggaaa cagtcatatg ctgccgaaac ctcccggaga aatgaaaacg
     7921 gagaggagag ccgggctccc ctctccctgc tcccgccggc taatcagcgg atgtggaaag
     7981 atccttgtac ttagagtcgg tgatggtaac atcggcctca agcctgagct ccgaagcaag
     8041 cgcctgagcg ctcttggcct gattcccttt tatggtccgc tcgatgtcct ccttcacctc
     8101 gtcgaatgta gcctcatgcg cgtctttcct ttcggtgaca cggatgatgt ggtatccgta
     8161 ctcggacttg accggcgcgc tggtctggcc gggcgacatc gcaaaagctg ccttctcgaa
     8221 ctcggccacc atg
//
LOCUS       DAOTAC010000089         8216 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_7422_length_8216_cov_4.036638, whole genome shotgun sequence.
ACCESSION   DAOTAC010000089 DAOTAC010000000
VERSION     DAOTAC010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 8216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8216
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7422_length_8216_cov_4.036638"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="PLM74_05185"
     CDS             complement(<1..242)
                     /locus_tag="PLM74_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009362632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-protein-alanine N-acetyltransferase
                     RimI"
                     /protein_id="HON42513.1"
                     /translation="MEHTVSLMTLADVDDVVEIERQSFPIPWSRAAFIDELTRNERAL
                     YIVTRDESGTMTGYGGMWLVCDEAHVTNIAVHAGRRR"
     gene            complement(247..1002)
                     /gene="tsaB"
                     /locus_tag="PLM74_05190"
     CDS             complement(247..1002)
                     /gene="tsaB"
                     /locus_tag="PLM74_05190"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HON42514.1"
                     /translation="MLILAMDTSTMVGGVALLKNGVTVGEHILNVRATHSERLMSSVQ
                     ALMSAAGVGPGDLDAIACGVGPGSFTGVRIGVSACKAMAYALSIPIVSVPSLDALAYG
                     RGRGARAVWCLIDARHERCYSASFVPPESGDVWWPERASAYAVRYVGEVATLARETAS
                     ALASSVSGDSGRPERVLFLGDGALAYAESLRSELPGVAEIPPAPMCMLRPSQVGLLGF
                     DLLSAGIADDPASVVPMYLRPSEAEVRLAEGKR"
     gene            complement(996..1466)
                     /gene="tsaE"
                     /locus_tag="PLM74_05195"
     CDS             complement(996..1466)
                     /gene="tsaE"
                     /locus_tag="PLM74_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015759131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HON42515.1"
                     /translation="MTETVRIISHSPAETIAIGCGLGKLLQPGDVLCLAGGLGAGKTC
                     LARGIAIGLGADEARVSSPTFVLAQEYRGRIPMYHLDLYRLTSAEEVEEAGLDEYVGG
                     DGVAVIEWPDVYSPLLNMDRLTVEIRTTATFERELVLTPQGDRYRSILEEMRPC"
     gene            complement(1510..2559)
                     /locus_tag="PLM74_05200"
     CDS             complement(1510..2559)
                     /locus_tag="PLM74_05200"
                     /inference="COORDINATES: protein motif:HMM:NF017864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HON42516.1"
                     /translation="MSQALVSLYRGELVESVHRGHIAVTDAEGRLLWWAGDPGCVTYF
                     RSSAKPIQVLPIVASGAVDRFGITDTELAVMAASHRGEDIHIDAVTSILGRIGLTESA
                     LMCGTHAPSCVWPPERVSAKCSQAAGRIRSGASFSPVFNNCSGKHAGMLMYAVHRGYP
                     IDDYVSRDSRVQQDMWQAVSDVAGLPREEVVRGVDGCGVVVFGMPLSGMARAYARMSA
                     PESLPVEYVEPARRIVQAMMDHPEMVGGSGDFGTDLMLGARGALFCKGGAEGLFCVGI
                     PGKGVGIAVKIEDGGARALPSTVVEILRVLGAVTPEMSGMRELAIERPVRNCRGDVVG
                     YMRPCFKLERASGTI"
     gene            complement(2570..3295)
                     /locus_tag="PLM74_05205"
     CDS             complement(2570..3295)
                     /locus_tag="PLM74_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HON42517.1"
                     /translation="MRPLILVSADCDIESSDSFKLQADYVRAVYLAGGLPLVAVPPME
                     CCDTAEAVAELCSLARGIVLTGGSDVLPGLFGEEPHPKLGAVNPVRDRFELALARAAM
                     QADLPVLAICRGMQVLNVAAGGGLVQDIESCVPGAHAHRVTAPRWLPTHSVSVVPGSI
                     LWKIFRRDSIMVNSFHHQAVHPVAPGFEVSARASDGVVEAIEKAGSRFMVGVQWHPEA
                     LAGKDPEALNVFREFLIAAQGVY"
     gene            complement(3352..3702)
                     /locus_tag="PLM74_05210"
     CDS             complement(3352..3702)
                     /locus_tag="PLM74_05210"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HON42518.1"
                     /translation="MDIKRGDVFYADLNPVVGSEQGGIRPVLVVQNDIGNRYSPTTIV
                     AAVTSRIKRAKLPTHVELPASSGNLAFDSVVLMEQLRTIDKARLLQHVCTLDGDTMRK
                     VNRAAEISLGLMDI"
     gene            complement(3704..3979)
                     /locus_tag="PLM74_05215"
     CDS             complement(3704..3979)
                     /locus_tag="PLM74_05215"
                     /inference="COORDINATES: protein motif:HMM:NF024064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HON42519.1"
                     /translation="MAELKRIMVSVPSSLLSEVDVFSKTIGLNRSEVVREAVRMFMAE
                     HARSELGRKLKEGYSEMASINSQMADECAAAEEEAFIRYERFLAGGD"
     gene            complement(4143..5261)
                     /gene="alr"
                     /locus_tag="PLM74_05220"
     CDS             complement(4143..5261)
                     /gene="alr"
                     /locus_tag="PLM74_05220"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HON42520.1"
                     /translation="MRPTWVEVDTGSIAKNIERVRATIPGKTQIMAVVKADGYGHGAV
                     PVAIAALKSGAAFLGVALVEEGIALRRAGIVAPILFMEALFPWQAPDLVRYGLTATVA
                     SYESADVLSESAALLGRKAKVHIKVDTGMGRLGLPPEAIPGLVRHVSGLPRLEIEGIF
                     THLACAESDPSMTCRQLELFHDIVDGLAAEGYEIPIKHVANSAAALRVPEAAMDMVRI
                     GGAMYGISPLGISPLGQGWQRALSFKTRVSYMKPVKAGATVSYGATYVAPGPQLIATL
                     PVGYADGYSRLLSGRGEVLIGGVRCPVAGRVCMDQIMVVLPPDSDVRVGDEAVLIGEQ
                     GNEVICAEDIARIMGTIGYEVVCAISKRVPRVYVSSQP"
     gene            complement(5292..6929)
                     /locus_tag="PLM74_05225"
     CDS             complement(5292..6929)
                     /locus_tag="PLM74_05225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HON42521.1"
                     /translation="MKLATPDEMRQIDRAAAERYGVSGLALMENAGAAVAKVAFSVAT
                     RASRAVRAGVCGRDQDEPAPPVSVCILAGRGNNGGDGFAAARHLAGYGARVSVYVVGD
                     PSTISGDARTNLDAMMKSGICVVPLLDAGAWSAAREDLAASSVIIDAILGTGARGAPS
                     PEAARAISLINESPAPVVAVDVPSGMDALTGAVPGECVIAHTTVTLALAKTGLATYPG
                     ASCAGRVVVDGIGMPPPLLDFSEIRTGLTTPDDVRAALPVRRPDAHKGDFGRVLIVAG
                     SPGMSGAPSLAAMGALRSGAGLVHLLVPAAIAGEVSARHAEVMTHPAPEPCLPAILEM
                     AAACQAVVIGPGMSCCERTSAVVDAVVSQVRVPLVLDADAINVVAGKARALREAKAQV
                     VLTPHPGELGRLMGVGADEINRDRLGWARRAALETGATVVLKGARTVIADQSGEAYVN
                     PTGNSGMATAGMGDILAGVTGSLCAVGLDGLAAGYCSAYLHGLAGDFAARERGTDGMV
                     AGDVLVHVPESFTAVRTKMAMLWSMMPIAYTHMSEGW"
     gene            complement(6910..7404)
                     /gene="acpS"
                     /locus_tag="PLM74_05230"
     CDS             complement(6910..7404)
                     /gene="acpS"
                     /locus_tag="PLM74_05230"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HON42522.1"
                     /translation="MSQPSFRQNLPDSVIGVGVDIIRTERFEEIAQRAPGGVAARLFT
                     PGELSSRRANDREYLASRFAAKEAVMKSLGCGMGDVSFTDIEVYHTDGGAPAVGLSGT
                     ASRRAHEVGALRVLLSVSHDGDYVCAFAVALGHARGDGGRESVSASVSGIGSGGGDDE
                     ARDS"
     gene            complement(7370..>8216)
                     /locus_tag="PLM74_05235"
     CDS             complement(7370..>8216)
                     /locus_tag="PLM74_05235"
                     /inference="COORDINATES: protein motif:HMM:NF014879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HON42523.1"
                     /translation="PRELSLDGLRVVIDCANGASCRTSPAAFRALGARTTVINDSPDG
                     DNINVRCGSTHPEALQEAVRELNADLGFAHDGDADRVMAVDSDGNVVDGDQIMAVCAG
                     FRASSGGLPGGAIVATQYSNLGLVQAMRAIGCQVIMANAGDKYVLQEMRRRGLVLGGE
                     QSGHIIMLEKTTTGDGLITAVEVASIVAATGKPLSELASVMVKFPQIMVNVRVSRKDE
                     YGASASIRAAVEEGQAMLGDSARLVVRPSGTEPLVRVMGEGPDEAVVRKVVDGVAAVI
                     SAELA"
BASE COUNT         1678 a         2847 c         2184 g         1507 t
ORIGIN      
        1 cggcggcggc ctgcgtggac tgctatgttg gtcacgtggg cttcgtcgca taccagccac
       61 attccgccgt aacccgtcat cgttcccgac tcgtcacgag tcacgatgta gagagcccgt
      121 tcgttccggg tgagttcgtc gatgaacgcc gcacggctcc acggaatcgg aaaggactgc
      181 ctctcaatct cgaccacatc atccacatcg gcgagggtca tgagcgatac cgtatgttcc
      241 atctcgtcac cgcttcccct ctgcaagcct cacttctgcc tcggacggcc tgaggtacat
      301 aggaaccaca ctggcaggat catctgcgat ccccgcgctt agcagatcaa agcctaagag
      361 gccaacttgc gacggccgca acatgcacat gggcgcaggc ggaatctcgg ccacgccggg
      421 caactccgag cggagcgatt cggcgtacgc cagggctccg tctccgagga aaagcacccg
      481 ttccgggcgc ccactatcgc ccgataccga cgatgccagc gctgatgccg tttctcgggc
      541 aagggtcgca acctcgccga cgtacctaac tgcgtaagcc gatgccctct cgggccacca
      601 cacatcgcca gattcagggg gcacaaacga agcagaataa catctttcat gcctggcgtc
      661 gatcaagcac cataccgcgc gcgcccctct gccacgcccg tacgccagcg catcaaggga
      721 cggcactgac actatgggta tggacagagc gtacgccatt gctttgcacg ccgacacgcc
      781 tatgcggacg ccggtgaatg accctggccc tacgccgcac gctattgcgt caagatcgcc
      841 cgggccgacg cctgccgccg acatcagcgc ttgcactgat gacatcagcc gctcagagtg
      901 agtcgcccga acgttcaata tgtgctcgcc cacggtgacg ccgtttttga gcagggcgac
      961 gcctccgacc atagtcgaag tatccatcgc caggatcagc acggtctcat ctcctccagg
     1021 atgctcctat atctgtcgcc ctgaggcgtc aggaccaact cacgctcaaa tgtagcagtc
     1081 gtacggatct ccaccgtgag ccggtccata ttgagcaatg gactatagac gtccggccat
     1141 tcgatgacag ccacgccgtc gccgccgaca tactcgtcca gccccgcctc ctcaacctcc
     1201 tcggcagacg tcagcctgta caagtcgagg tggtacatgg gaatcctccc cctgtactcc
     1261 tgcgcaagta caaacgtggg gctcgacacg cgcgcctcat ctgctcccaa cccgatggct
     1321 atgcccctgg caaggcaagt ttttccagcg cccagccctc ctgcgaggca cagaacatcg
     1381 ccgggctgaa gcagtttgcc gaggccacag cctatggcga tggtctcggc aggcgagtgc
     1441 gatattattc gaacggtttc agtcatggta ttcaaccttc atgattatac ttgaccgact
     1501 cacggtcggt caaatcgttc cagaagccct ttccaacttg aaacacggtc gcatgtagcc
     1561 aacgacatcg cctctgcagt tgcgcacggg tcgttcgatt gcaagctcgc gcatgcccga
     1621 catctcgggc gtgactgcgc ccaatacccg caggatctcg acgacagtcg atgggagagc
     1681 tctcgctccg ccatcctcga tcttgacggc gatgcccacg cccttgccgg ggatccccac
     1741 gcagaagagg ccctctgccc ctcccttgca gaacagcgcg ccccgtgctc ccagcatgag
     1801 atcggttccg aagtctcccg acccgcccac catttcagga tggtccatca tggcctggac
     1861 gatcctcctg gccggctcca catattccac cggcagagac tccggcgccg acatcctcgc
     1921 atacgcccgc gccatgcccg agaggggcat tccgaacacc actactccgc acccgtctac
     1981 tccgcgtaca acttcttcgc gcggcaggcc ggccacatcc gacaccgcct gccacatgtc
     2041 ctgctgcact cgactatctc gagatacgta atcatcgatg gggtaacccc gatgcaccgc
     2101 gtacatgagc attccggcat gcttcccgga gcaattgttg aaaaccggac tgaatgaggc
     2161 gccggaccgg atccgcccag ccgcttggct gcattttgcg gagacgcgct ccggcggcca
     2221 cacgcacgac ggcgcatgcg tcccgcacat cagagccgat tcagtcagcc ctattctgcc
     2281 cagtatgctc gtgaccgcgt caatgtggat atcctctcca cgatgggacg cagccatcac
     2341 cgccagctca gtgtcggtga tgccaaatcg atcgaccgcc cccgaagcga ctatgggaag
     2401 tacctggatg ggctttgccg aagagcggaa gtaggtgaca cagcctggat caccggccca
     2461 ccacagcagc cttccctcgg catctgtcac cgctatgtgt cctctgtgca cactctcaac
     2521 tagctcgcct ctgtacagcg aaaccaacgc ttgcgacatc gaatgcaatt cagtacactc
     2581 cctgtgcagc aattaggaat tctctgaaca cattgagcgc ctccgggtcc ttgccggcaa
     2641 gcgcctccgg atgccactgg acgcccacca tgaaccggct tccggccttt tctatggcct
     2701 ccacaacccc gtcgctcgcc cgtgccgaga cttcgaaacc tggggccaca gggtgcaccg
     2761 cctgatggtg gaagctgttt accatgatcg aatcacgcct gaatatcttc cacagaatcg
     2821 aaccgggaac cactgatacc gaatgcgtag gcagccatcg cggagccgta acgcggtgcg
     2881 cgtgagcgcc cggaacgcac gattcgatat cctgaaccag accgccccct gccgccacgt
     2941 tcaatacctg catcccccgg caaatcgcga ggaccggcag atcggcctgc atggcagctc
     3001 gagccaacgc cagttcgaac cggtccctaa cagggttgac tgcacccagc tttgggtggg
     3061 gctcttcgcc gaacagacca ggtagaacat cggacccgcc cgtgagcacg atccctcgcg
     3121 ccagggagca cagctcagcg actgcctcag ccgtatcgca gcactccatg gggggtaccg
     3181 caacgagagg aagcccgccg gccaggtaga ccgctcgcac gtaatccgcc tgtagcttga
     3241 acgaatcgct cgactcaatg tcgcagtcgg ccgaaaccag tataagcgga cgcacgggaa
     3301 aaccacccca ttacgaactt gtcccggcag ggcttgcgct gccgggcaat actagatgtc
     3361 catcagcccg aggctgatct cagccgcacg gttcaccttt ctcatggtat caccatcaag
     3421 agtgcataca tgttgtagca ggcgggcctt gtcgattgta cgcagctgtt ccatgaggac
     3481 aacggaatca aaagcaaggt tgccagaaga ggccggaagc tcaacgtgag tcggcagttt
     3541 ggcccttttg atccgcgagg taacggcggc tactatggta gtggggctgt aacggttgcc
     3601 gatgtcgttc tgaaccacaa gcaccggccg gatcccgccc tgctctgagc cgactaccgg
     3661 gttgagatcg gcgtagaaga catcaccgcg cttgatgtcc aagtcagtca ccgcccgcaa
     3721 gaaaccgctc gtacctgatg aaagcctctt cctccgctgc ggcacactca tccgccatct
     3781 gcgagttgat cgaggccatc tcagagtagc cttccttcag cttcctgccc agttccgatc
     3841 gggcgtgttc cgccatgaac atccttacag cttcgcgcac gacttcgctt cgattgagcc
     3901 cgattgtctt gctgaaaaca tccacttcgc tcaggagcga agacggaacg gagaccatga
     3961 tgcgcttgag ttctgccaaa gcaatacccc ccgaagcgcc ggctcatatt cccgcatatt
     4021 cgtatgcatg ccaattatac atgtatgcct aaagccatgt caacctgcgg agatactgca
     4081 cgatactggg tattccgccc gtggttccgc ccgtagttcc atccgcggac cgctatgcac
     4141 cgtcacggct gcgatgacac atacacgcgc ggcacccgct tgctgatggc gcacacgact
     4201 tcatagccta tggtgcccat gatccgcgct atatcctcag cgcatatcac ttcattgcct
     4261 tgctccccaa tgagcactgc ctcgtcgccg acccgtacgt cggagtcagg gggcagaacg
     4321 accattatct gatccatgca cacgcgacct gcgacagggc agcgcacgcc accaatgagc
     4381 acctcccccc gccccgatag caggcgtgag tacccatctg catagcccac gggcaatgtt
     4441 gcgatgagct gaggaccggg cgcgacatag gttgctccgt agctaaccgt ggcgccggcc
     4501 ttcacaggtt tcatatacga taccctggtc ttgaaagata gcgcccgctg ccatccctgg
     4561 cccaggggag atatgcccag cggggatatg ccatacatag cgccgccgat cctgaccatg
     4621 tccatggcgg cctccggaac gcggagcgct gccgcgctgt tcgccacgtg cttgataggg
     4681 atctcgtatc cttccgcggc gaggccgtcc acgatgtcgt ggaacaactc cagctgcctg
     4741 cacgtcatgg atgggtcgct ctctgcgcac gccaggtggg tgaatatgcc ttcgatctcc
     4801 agtcgcggca ggcccgatac atgcctgacc agcccgggaa tggcttcagg gggtaacccc
     4861 aacctgccca tgccggtgtc taccttaata tggaccttgg ccttccgccc aagtaaggcg
     4921 gctgattctg acaacacgtc cgcagattca tacgatgcca cggtggcagt caggccatac
     4981 ctgacgaggt cgggcgcctg ccacggaaag agggcctcca tgaacagtat gggcgcaacg
     5041 atgcctgccc ttctcagggc gattccctct tccaccagag ccacacccag aaaagcggcg
     5101 cccgacttca gagccgcaat tgccactgga acagctccgt ggccgtatcc gtcagccttc
     5161 actaccgcca tgatctgcgt cttgccaggg atggttgccc ttacgcgttc gatattcttc
     5221 gcaatgctcc cggtgtctac ctcaacccag gtgggtcgca taccgggtag ataagtctct
     5281 ttcagccagg gtcaccatcc ttctgacata tgcgtatatg caatcggcat catgctccaa
     5341 agcatggcca tcttcgtacg cactgctgta aatgactcgg gaacgtgtac cagcacatcg
     5401 ccggcgacca tcccatccgt accgcgctcc cgcgctgcga aatcacctgc caggccatgg
     5461 aggtaagccg agcaatatcc tgccgcaaga ccgtccagac ccacggcaca cagactccct
     5521 gttacgcccg ccagaatatc gcccattccg gccgtggcca ttccagaatt gccagtgggg
     5581 ttcacatatg cctccccact ctggtcggca atgaccgtcc ttgcgccctt cagtaccact
     5641 gtggcgcctg tctccagcgc tgcccgccgg gcccacccca ggcgatcccg gttgatttcg
     5701 tctgctccga cgcccatcag tctacccagc tcgcccgggt gcggagtgag aacaacctgc
     5761 gctttggcct ccctgagcgc ccgagccttc cctgccacga cgttgattgc gtcggcgtcc
     5821 agcaccagcg gaacgcgaac ttgcgaaacc accgcatcca caactgccga agtgcgttcg
     5881 caacacgaca ttcccgggcc aatgactact gcctggcaag cggcggccat ctcaagtatg
     5941 gctggcaagc acggttcagg cgccggatgc gtcatgacct cggcatgcct cgcgctcacc
     6001 tcgccagcta ttgcggcagg aaccagcagg tgaaccagtc ccgccccgga ccttagggcc
     6061 cccattgcag ccagcgacgg agccccggac atcccgggcg agccggcgac gatcagaaca
     6121 cggccgaaat cgcccttatg agcgtcgggc cgccgcacag gcaaagctgc acgtacatcg
     6181 tccggcgtcg tcaggcctgt tcttatctcg ctgaagtcga gtagtggggg cggcatgccg
     6241 atgccatcca caaccacacg gcccgcgcaa gacgccccgg gatacgtcgc tagacccgtc
     6301 ttggccaagg caagtgtgac cgtggtgtga gcgatcacgc attccccggg cacagcgccg
     6361 gttaaggcgt ccataccgct gggaacgtcc acagctacaa caggcgcagg cgactcgtta
     6421 ataagcgata tagcccgtgc tgcctcaggc gacggcgccc cgcgcgctcc agtgcccaag
     6481 atagcatcga tgatcacact agatgctgcc agatcctccc gagcggcgct ccacgccccc
     6541 gcgtccagca gtggtacgac gcagatcccc gatttcatca tcgcgtccag gttggtccga
     6601 gcgtcccccg atattgtaga cggatccccc accacgtaga cggagacgcg agcgccgtac
     6661 ccagccaggt gacgagcagc ggcaaagcca tcgccgccgt tgttgcctcg acccgccagt
     6721 atgcagacgg ataccggagg cgccggttca tcctgatctc ggccacacac accagcccga
     6781 acagcacgag aggcccgagt ggccacgcta aacgccactt tggctacagc cgcgcccgca
     6841 ttctccatca gggctaaccc agaaacgccg tagcgttcgg cggccgcccg atctatctgc
     6901 cgcatctcgt caggagtcgc gagcttcatc atcgccaccc ccgcttccga tcccgcttac
     6961 gctcgcactc acgctttctc tcccgccgtc gccccttgca tgccccagcg cgacggcaaa
     7021 ggcgcacaca tagtcgccat cgtgcgacac tgacagcagc acacgcagag ctccgacctc
     7081 atgtgcccgg cggctggccg tgcccgagag cccgaccgca ggcgcacctc catctgtatg
     7141 gtagacttca atatccgtaa aggagacatc gcccatgccg cagccaaggc tcttcatcac
     7201 agcctccttg gccgcaaacc tggacgcgag gtattctcgg tcattggctc tgcggctaga
     7261 tagctcgccc ggcgtgaaca ggcgcgcggc caccccgccc ggcgcacgtt gcgctatctc
     7321 ctcgaatcgc tccgtacgta tgatatcgac acccacacca atgacgctat caggcaagtt
     7381 ctgccgaaat gacggctgcg acaccatcaa caaccttcct cacgacggct tcatccgggc
     7441 cttcgcccat gacgcggacc aacggctcag tgcctgacgg cctcaccaca agccgagctg
     7501 aatcgcccag catggcctga ccctcctcta cagccgctcg tatcgaggcg gaggcgccat
     7561 attcgtcctt gcgcgacaca cgcacgttca ccatgatctg cggaaacttc accatcaccg
     7621 aagccagttc gctcaagggc ttgcccgtag ccgccacaat ggaagccact tcgaccgcgg
     7681 tgatgagtcc atcgcctgta gtggtctttt ccagcattat gatatgcccg gactgctcgc
     7741 ccccaagcac cagaccgcgg cgcctcatct cctgaagaac gtacttgtcg cccgcgttgg
     7801 ccatgatcac ctgacaaccg atggctcgca tggcctgaac caggccgaga ttgctgtact
     7861 gcgtcgccac gatagcgccg cccggcaacc ccccgctcga ggcgcggaaa cccgcgcaca
     7921 ccgccataat ctggtcccca tcaacaacgt tcccatcgga atcgactgcc atgacacgat
     7981 cagcatcgcc atcgtgggca aaccccaggt cagcgttgag ctcacgcact gcttcctgga
     8041 gtgcctcagg atgagtcgat ccgcaacgga cgttgatgtt gtcgccgtcc ggggaatcat
     8101 taatcacagt cgtcctggcg cccagtgccc gaaaagccgc gggagaggtg cggcaggacg
     8161 cgccgttggc gcaatcgatc acgacacgca gaccgtcgag cgagagctcg cgcggc
//
LOCUS       DAOTAC010000090         8175 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_7466_length_8175_cov_4.884360, whole genome shotgun sequence.
ACCESSION   DAOTAC010000090 DAOTAC010000000
VERSION     DAOTAC010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 8175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8175
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7466_length_8175_cov_4.884360"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..953)
                     /locus_tag="PLM74_05240"
     CDS             complement(<1..953)
                     /locus_tag="PLM74_05240"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HON42524.1"
                     /translation="MSSRESAYERKLFGFGAVINEYWNDEMRRKIALDYDADKKIFIR
                     AKSISGLFTLLLSVIALVLLMVVRENLMTAGVYISLIIAFSRLIHTLSWQLSDLLKEH
                     TKKRLYLEDLSRFERLEELPGSDSVPDKRILDMPFESLVFDHVSFTYPQSDREVLHDF
                     CLAVYPGKQYAFVGRNGCGKSTVIKLLTGLYNDYSGRILLNGRDIRSLSAAEIKAYFA
                     VVYQDYGKYQLPAREQLQLGNASLADPEIAALLEQTGLAQKVGSLPLGADAPLGKLEP
                     GSADLSEGQWQRLTLARSFGRQAKLLVLDEPAAALDALAESR"
     gene            complement(996..1580)
                     /locus_tag="PLM74_05245"
     CDS             complement(996..1580)
                     /locus_tag="PLM74_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42525.1"
                     /translation="MIGFSGFANAEFIKIAFGCSAPLTVAVAALRLVAALTPSLETLA
                     VAGLLDALPGAVANGRVVDALTPLLALFAVLLCSYVVQVLNRFLMLKLSMRLEDRLKP
                     EFVAKVSRLAYEHIENDATYDLLERVGADLPGAVAGGFASLLGLAEISLRVLGPVLII
                     GRSSVPAALACLLLIIPVAVLAQRSGAEAYQAGA"
     gene            complement(1682..3220)
                     /locus_tag="PLM74_05250"
     CDS             complement(1682..3220)
                     /locus_tag="PLM74_05250"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HON42526.1"
                     /translation="MREGRAAHLALAVCVTIALLLAGPSSGQTARLVLEPTARAIAQA
                     NRPDASRMYDAEMTTAFARLLYPYRDFADELGLEELGGVPTSPVNRPSTISPEDATWE
                     VDWVFRVLKYGYAGYQCFGGDEAFLAAKGRILQDLGERSSDLSTEEYESLICGHLGFI
                     QDGHLSLGGTRLCEFYSLFWNPKYEFRRDDAGFFIAASDGIRRAASIEGVDPCSYLKP
                     SISETGDIIYVPGVVRPRLDAGFTINVKYRDGHSEAIRLDPMVSTAQGGPAYELGAID
                     GIPLLACRSFAPSDANLDALNAFVDDASRLRDERVIILDIRSNPGGSSQYPTAWLWRL
                     MYQLPREQQIYANLTTETAVALLGNLLSSYSDAAQMPKNAATSLRRDASSSRPGWSNI
                     LHAAGAVVNNEPSIAVITDSYIASAAEDLVLGLRHADSVVFIGANTRGALSTGTVGMC
                     WLPISRLSMRVPVVLNRVLDMTNRDGLGLFPDFWVDPNNAVERAVRFLRKHLAPDSPE
                     QPQR"
     gene            complement(3233..4576)
                     /gene="hydF"
                     /locus_tag="PLM74_05255"
     CDS             complement(3233..4576)
                     /gene="hydF"
                     /locus_tag="PLM74_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HON42527.1"
                     /translation="MLRTSLDKTPMSNRIHIAVLGRMNSGKSSLINAICGQDVSVVSP
                     IAGTTTDIVHKAMEIHGLGPVVFIDTPGIDDESVLAEARIARTQRAINKADMALVVID
                     ATCGVGEPESALLAELGMKRIPYIVAINKADIAPVAGSDADDLSRSADSSGWGDSAYG
                     CSSSVAGCAQRVDDHRSHALAKDVSRVSVSALTGEGVIQLIEYIRTLASQAEVWDGPP
                     IVGDLLAPGDTVVLVIPEDAEAPKGRLILPQVQTIRDILDHNAVATAMRVAQLETVFA
                     DRSFRPRLVITDSQAFREVDAIVPSFVDLTSFSILFARHKGDLELLARGARVIDSLEP
                     GDSVLIVEACTHHPVDDDIGTVKIPRLLNRKAGGELSFEWRRGADFPADLARFKLVVH
                     CGGCMLNRREMVSRLSAVEDTGVPVTNYGMTIAACLGILPRALRPLGVAMEKTPV"
     gene            complement(4828..6219)
                     /gene="hydG"
                     /locus_tag="PLM74_05260"
     CDS             complement(4828..6219)
                     /gene="hydG"
                     /locus_tag="PLM74_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012994703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HON42528.1"
                     /translation="MPRDGVECTFIDDKTIARALEQGKRYSPTAVRNVLAKSLELHGL
                     DLEELAALLWVDDSELLEEVYAAARKIKETIYGKRLVFFAPLYVSDYCVNNCTYCAYK
                     RDNKYPRRKLNMDEVRDEVRAIERMGHKRIALEAGEDPANCTIDYITDVMHTIYDTHC
                     DNGSIRRINVNIAATTVEDYRRLKDAGIGTYVLFQETYHRDTYRQVHDGPKADYDWHT
                     TAMDRALTGGIDDVGIGVLFGLYDWRYETIALLQHAHHLDDAYGVGPHTISFPRLRPA
                     AGMSLDEFPHLVSDADFKRIVACLRLAVPYTGMILSTREAPEFREEVIALGVSQISAG
                     SCTGVGEYSRGEAHDTPQFEVSDHRDLDQIIRDLLPSGYVPSFCTACYRSGRTGEAFM
                     SLAKNSHIHEFCQPNALLTFREYLCDYASPDTVKLAEPAIRRAFAEVDPRLRQECERR
                     LKRIISGERDLYF"
     gene            complement(6186..7481)
                     /locus_tag="PLM74_05265"
     CDS             complement(6186..7481)
                     /locus_tag="PLM74_05265"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HON42529.1"
                     /translation="MAKTESEVAAALDMPGPDREMALWADAHCARVAAVGPCVHLRAI
                     IEFSNYCRQDCLYCGLRRSNAKVARYRMDPDEIVEAAVAAWRVHKFGTFVLQSGEDPG
                     YPPEDLARAIGRIKTSTNAAVTLSVGEAPDADYRLWREAGADRFLLKFETSDPDLFAM
                     LKPTTTLAARMGRLQALRDMGYQVGSGIILGLPGQDRISVARDLILCGEEEYEMVSIG
                     PFVPHPDTPLGRRVRDHGQTQASAAALAPARTPDVRATLSTIAVARLLTPMAHIPATT
                     ALGVVGRQDGAWRGQAASYIATMGVVGGTGRDGGDRDRQDYTSSPLCDSGREHTEYSG
                     DARYLALFAGANVLMLDVTPWEYRNYYEIYPGKPGADGDELASSVNSAIREIRRLGMS
                     VCPGRGDSPKAPWGRAARKGEHTEGRQPRCREMELSARS"
     gene            complement(7462..7770)
                     /locus_tag="PLM74_05270"
     CDS             complement(7462..7770)
                     /locus_tag="PLM74_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HON42530.1"
                     /translation="MASSGEKGRTGGPRGENRVGVAAIVVQDRLNAASAVNRILSDYA
                     DIIVGRMGIPYRDRGVSVMALILDGDTDTIGAMTGKLGSIAGVKSRVTLVNLDGKDGE
                     "
BASE COUNT         1690 a         2587 c         2248 g         1650 t
ORIGIN      
        1 cggctctcag ccagagcgtc aagggccgcc gcaggttcgt ccagcaccaa gagcttggcc
       61 tgtcttccga agcttctggc caaagtcagc cgctgccact gtccttcaga cagatccgca
      121 ctgccaggtt cgagcttgcc caaaggggcg tcggcgccca ggggaagcga ccccaccttc
      181 tgcgccagac ctgtctgctc aagcagggcc gcgatctccg gatcggcaag actcgcattg
      241 cccagctgca gttgttctcg cgccggcagc tgatacttgc catagtcttg ataaacaaca
      301 gcgaaatacg cctttatctc agcggcggaa agcgacctga tatctcgacc gttcagcaga
      361 atccggccgc tgtagtcatt gtagagcccg gtgaggagct tgatgaccgt ggacttcccg
      421 cagccgttcc gaccgacgaa cgcgtactgc ttccctgggt agacggcgag acagaagtca
      481 tgcaggactt ccctatcgga ttgagggtat gtgaaactga catgatcgaa aaccagactc
      541 tcaaagggca tgtcgagaat gcgtttgtcc ggaacgctgt cacttcccgg cagctcttca
      601 agccgctcaa atcgactaag gtcttccagg tacagacgct tcttcgtgtg ctccttcagc
      661 aagtccgaga gctgccacga cagggtatga atcagacgag agaaagcaat gatcaggctg
      721 atgtagacgc ctgccgtcat caggttctct ctgacgacca tcagaaggac cagggcgatg
      781 acagacaaaa gcagtgtgaa caggccgctg atgctcttgg ccctgatgaa gatcttcttg
      841 tccgcatcat agtccaaagc tatcttccgg cgcatctcgt cgttccagta ctcgttgatt
      901 accgcgccga atccaaacag cttacgctca taggcagatt ctcttgagga catgagagac
      961 ttaaggtatc tggcccttct ggcgtgaggt gcgacctagg cgccggcctg ataggcttca
     1021 gcgccgcttc tctgagcgag aaccgcgacc gggataatga ggagcagaca ggcgagcgca
     1081 gccggcacag aagatcggcc gatgatcaag acagggccca gaacgcgcag gcttatctca
     1141 gccagaccca agagactcgc aaaccctccc gccacggcgc ccggaagatc cgcgcctacc
     1201 cgttccagca aatcgtaggt cgcgtcgttt tcgatatgtt cataggccag acgactgacc
     1261 ttcgcaacga attcgggctt cagacggtcc tccaatcgca tgctcagctt cagcatcagg
     1321 aagcggttaa ggacctgaac aacataggaa catagcagaa cagcaaacag ggcaaggagc
     1381 ggcgtcaggg cgtccacaac acgcccgttc gccacagcgc cgggcagggc atcgagtaat
     1441 ccggcgacag ccaacgtctc cagcgaaggc gtcaaggctg caaccaaacg cagagcagcc
     1501 acggccacgg tcaaaggggc gctgcagcca aaggcgattt tgatgaattc tgcattcgca
     1561 aagccactaa acccaatcat gtcttcctat ccaagcctct ccaagcctct ctgaccgcgg
     1621 ccaatcgccc atcgccaatc agctgcgctg caccgctatg agcgccgggc gaacgagggc
     1681 ttcatcgctg cggctgctcg ggactatccg gcgccaggtg tttgcgaagg aatctcacgg
     1741 ccctctccac agcattgttc ggatcaaccc agaaatccgg gaacagcccc aatccatctc
     1801 ggttcgtcat gtccagaacg cgattcagca ctaccggcac tctcatgctc agccgggaaa
     1861 tgggcagcca acacatcccc acagtgcctg tggacaaagc gcccctagta ttcgcgccta
     1921 tgaacaccac actgtctgcg tgacgcagac cgagcacaag atcctcggca gccgaggcta
     1981 tgtatgaatc cgtgatcacc gcgatagacg gctcgttatt tacgacggcg ccggccgcgt
     2041 ggaggatgtt gctccagccc gggcgggaac tgctggcatc gcgtcggaga gaggtcgctg
     2101 cgttcttggg catctgcgcc gcgtcagaat aggagctgag caagttgcct agcagtgcta
     2161 cggctgtttc ggtagtcaga ttggcataga tctgctgctc tctgggcagc tggtacatga
     2221 ggcgccacag ccaagccgtt ggatactgtg agctgcctcc gggattggag cgtatgtcga
     2281 ggattatgac gcgctcatcg cgcaggcggc tcgcatcgtc aacaaacgca tttagggcgt
     2341 caagattggc gtccgacggc gcgaagctcc tgcacgcaag gagcggaatg ccgtctattg
     2401 cgccaagctc gtacgcaggc cctccttggg cggtggagac catcggatca aggcgtatcg
     2461 cctcagagtg accatctcgg tacttcacgt tgatcgtgaa tcccgcgtca agtcttgggc
     2521 gcacaacacc tgggacatat atgatgtccc cagtctcgct gatagaaggc ttcaggtagc
     2581 tgcacggatc gacgccttcg atcgacgccg ctcgccgaat cccgtcgctt gcagcgataa
     2641 agaacccggc atcgtccctg cggaactcat atttggggtt ccaaaacagc gagtagaact
     2701 cgcacagcct ggtgccgccc aggctgaggt gtccatcctg aatgaaacct agatgaccgc
     2761 agatgaggga ctcatattcc tccgtagaca ggtcgctgga ccgctcgccc aggtcctgca
     2821 gtatccgccc cttcgcggcc aagaacgcct catcgccgcc aaagcactgg tagccagcgt
     2881 atccgtattt cagcactcga aagacccagt cgacttccca tgtcgcatct tcagggctga
     2941 ttgtggacgg tctgttgaca gggctggtcg gtacccctcc cagctcctcg aggccgagtt
     3001 catctgcaaa gtcacggtat gggtacagca gtctggcgaa ggccgttgtc atctccgcgt
     3061 catacatccg ggacgcatcc gggcgattgg cctgagcaat ggcgcgcgca gtaggctcca
     3121 gaacaagacg cgcagtctgg ccacttgaag gccctgctag aagcaaggcg atggtcacac
     3181 agacagcaag ggccagatga gcagctctgc cttctctcaa caatagccct cctcacaccg
     3241 gagtcttttc catcgccacc cccaatggcc gcagcgcacg cggtaggata cccaggcagg
     3301 cggcaatggt cataccgtaa ttcgtcacag gcactccagt atcctccact gcggacaggc
     3361 gtgataccat ttcccgccta ttcagcatac acccgccgca gtgtaccacc agcttgaacc
     3421 tggccaggtc ggcggggaag tcagctccgc ggcgccactc gaaggaaagc tcgcctccgg
     3481 ccttccggtt gagcaaccgg gggatcttca cggtacctat gtcgtcatcg acagggtgat
     3541 gcgtgcaagc ctcgactata agcacgctat cgccgggctc caatgaatcg ataacgcgcg
     3601 cgccgcgggc gagcagttcg aggtcgcctt tgtgacgggc gaagagaatc gaaaacgatg
     3661 tgagatcgac gaaactgggc acaatggcgt cgacttcgcg gaaggcctgc gaatctgtga
     3721 tgaccagtcg cggcctgaaa gaccgatcgg caaacaccgt ttcgagctgg gccactcgca
     3781 ttgccgtcgc aaccgcattg tggtcgagga tgtcccgtat cgtctggacc tggggcagta
     3841 tcaaccgtcc tttgggtgcc tcagcgtctt cagggattac gagcacaacc gtatcccccg
     3901 gagccagaag gtcgccgact ataggcgggc catcccacac ttctgcctgc gaagccagtg
     3961 tccggatgta ttcgatcagc tgaattacgc cttctccggt gagagcgctg acggatacgc
     4021 gtgagacgtc cttcgccaga gcatgcgatc ggtgatcgtc cacacgctgc gcacagcccg
     4081 cgaccgacga actgcaaccg tatgctgaat cgccccagcc cgatgaatca gccgacctcg
     4141 acaaatcatc cgcgtcactg ccggccaccg gtgctatatc ggccttgttg attgcgacga
     4201 tatacggtat ccgtttcatg ccaagctcgg ccaggagcgc gctttcgggt tcgcctacgc
     4261 cgcaggtcgc atcgataacg accagagcca tatcggcctt gttgatagct ctctgtgttc
     4321 gggctatgcg cgcctctgct aatacactct catcgtcgat tccgggtgta tcaatgaaga
     4381 ctaccggccc cagcccgtgt atctccatgg ccttatgcac gatgtcggtg gtggtgccgg
     4441 caatcggtga gactactgac acgtcttgcc cgcaaatagc gtttattaga ctggatttgc
     4501 ccgaattcat cctgcccagc actgctatgt gaatgcggtt cgacattggg gttttgtcca
     4561 aactcgttcg gagcatgtcg ttcctcctta acgccaacgc cggtacagat atcggaattg
     4621 cattccaaca ttcagccgtc tcaacgtgga atcgcgttcc accctctcag ccgtcgcgat
     4681 gtggaatctc gttctacctt ctccgccatg gctacgtgga atcgcatttt agagccatac
     4741 cggaacgaaa ttccgatatt aacgggatcg aaaatcacgc caatcgtcgc cagcccaggc
     4801 tacgtagcgt gaagtgctac aaagaaccta gaagtagaga tcacgttccc cgcttatgat
     4861 ccgcttcagc ctgcgctcac actcttgtct gagtcgagga tcgacctctg cgaacgcacg
     4921 cctgatagcc ggctctgcca acttgacagt atcgggtgac gcatagtcgc acaaatactc
     4981 ccggaaggtg agcaatgcgt tgggctggca gaactcgtgg atgtggctgt ttttggctaa
     5041 ggacatgaac gcctcgcccg tcctgcccga tctgtaacaa gctgtgcaga agctgggaac
     5101 atagccggac gggagcagat cgcgaatgat ctgatccagg tcgcgatggt cgctcacctc
     5161 aaactgcgga gtatcatgtg cctcgcccct gctgtactcc cccactccag tgcacgaacc
     5221 tgcgcttatc tgcgatacgc ccaaagctat cacttcctcg cggaactcgg gcgcctcgcg
     5281 tgtcgacaag atcatgccgg tgtatggcac cgccagacgc aggcaggcaa ctatccgctt
     5341 gaagtcggcg tccgatacca gatgcggaaa ctcgtcgagc gacatcccgg cggctggcct
     5401 gaggcgcgga aacgagatgg tgtgcggccc gaccccgtag gcgtcatcca ggtggtgcgc
     5461 gtgctgaagc agcgcaatgg tctcgtatct ccagtcgtac agtccaaaga gcacgccaat
     5521 tccgacatca tcgatgccgc cggttagcgc cctgtccatg gcggtggtgt gccagtcgta
     5581 gtcggccttg ggcccatcgt gcacttgacg atacgtgtcg cggtgatatg tctcctggaa
     5641 aagcacataa gtcccgatgc cagcgtcttt gagtctgcgg tagtcctcta cagtagtagc
     5701 tgcgatgttc acgttaatcc ggcgtatgct gccgttatcg cagtgcgtgt cgtatatcgt
     5761 atgcatgaca tcggtgatat agtcgatagt gcagttcgcc ggatcctcgc ccgcctcgag
     5821 ggcgatacgc ttgtgcccca tcctctcgat cgcccgcacc tcgtcgcgca cctcgtccat
     5881 attaagcttg cgccgaggat acttgttgtc gcgcttgtag gcacaatagg tacagttgtt
     5941 cacgcagtag tcgctcacat acagcggcgc gaagaagacg agacgctttc cgtatatggt
     6001 ctccttgatc ttgcgcgcag ccgcgtagac ctcctcaagc agctcggaat cgtccaccca
     6061 cagcaaagcc gccaattcct ccaggtcaag cccgtgcagt tcgagtgatt tggccaaaac
     6121 gttcctgaca gccgttgggc tgtagcgctt accctgttcc agggctctag caatcgtctt
     6181 gtcatctatg aacgtgcact caactccatc tcgcggcatc tgggttgtct cccttctgtg
     6241 tgttctcctt ttcgtgcggc acgtccccat ggagccttgg ggctgtctcc ccgcccgggg
     6301 cagaccgaca tgcccaaccg gcggatctcg cggatcgccg agttcaccga gctggccagt
     6361 tcatccccgt cggccccggg cttgcccggg tatatctcgt aatagttcct gtattcccac
     6421 ggcgtcacgt ccaacatcag cacattcgcc cctgcaaaga gcgcgaggta gcgcgcatcg
     6481 cctgagtact ctgtatgctc gcgcccggag tcgcacagcg gcgagctggt gtagtcctgt
     6541 ctgtcgcgat cgcccccatc ccgtccggtg ccgcccacca cgcccattgt agcgatgtaa
     6601 ctggccgcct gaccccgcca cgcgccatcc tggcggccaa ccaccccgag agctgtagtg
     6661 gccgggatgt gcgccatcgg agtcagtaga cgcgcaactg caatggtgct cagtgtggcg
     6721 cggacatcgg gcgtccgggc aggcgccaac gctgccgccg aggcttgcgt ttgcccgtga
     6781 tcacgcactc tcctgccgag cggggtatcg ggatgcggga cgaacggccc tatactcacc
     6841 atctcgtact cctcctcgcc gcacaggatg aggtcgcgag ctacggaaat gcgatcctgg
     6901 ccgggcaaac ccagtattat gcccgagcct acctggtaac ccatgtccct caacgcctgg
     6961 agtcgcccca tgcgcgccgc cagtgtggtc gtaggtttga gcatcgcaaa caggtcagga
     7021 tcgctcgtct cgaacttgag caggaacctg tccgcccccg cctcgcgcca cagccgatag
     7081 tctgcgtcag gcgcctcgcc cacgctcaat gtcacagccg cattggtcga cgtcttgatt
     7141 cgcccgatgg ctcttgctag atcctccggc gggtagcccg gatcctcgcc cgactgcagg
     7201 acgaaggtgc cgaacttgtg cactcgccac gcagccacgg ctgcctccac gatctcatcc
     7261 gggtccatcc ggtatcgggc gaccttcgcg ttgctccttc gcagcccgca gtagaggcaa
     7321 tcctgccggc agtagttcga gaactcgata atcgcacgaa ggtgcacaca cggaccgacc
     7381 gcggccacac gtgcgcagtg ggcgtctgcc cacagggcca tctcccgatc cgggcccggc
     7441 atatcaaggg ccgccgcgac ctcactctcc gtctttgcca tccaagttca ccaacgtgac
     7501 tcgggacttg acccccgcga tcgaccccaa cttgcctgtc atggccccga ttgtgtccgt
     7561 gtcgccgtcc aggatgagcg ccatcaccga cacgcccctg tccctgtacg gaatccccat
     7621 ccgtcctacg atgatgtcgg catagtcgct cagaattcgg tttaccgcag acgcggcgtt
     7681 cagcctgtcc tgcaccacaa tcgccgccac gccaactcgg ttttctcctc gcggaccgcc
     7741 cgttctcccc ttctcgcctg atgatgccat tttctctatc ccctccttct atcagcttcc
     7801 cacctcgccg gtgtcgcgat gtgcggaatg acgcggtttt gcccaatcaa aaaacccgcc
     7861 ttgcggacgg gttgataccc ctgagacgac aatgctctca ggaaagcgtc aaccctaggt
     7921 tcaggcacaa accctacccg caggtatcct cagtattcag atgatctaat cgtgcggcga
     7981 ccgaaggctc gaactcaagg tccgtggcca cacgcaatgc tattgtaaca cggtcctctt
     8041 gtgaaggaaa gcacaatccg ctcaggagct caggcgcccg aacctaggtg caatcacggc
     8101 gatcacttgc tcagcgtcat gtggtacaat ggacggtgga cgactgcctc gtgggcacag
     8161 tgccgacaac caagg
//
LOCUS       DAOTAC010000091         7999 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_7694_length_7999_cov_5.177996, whole genome shotgun sequence.
ACCESSION   DAOTAC010000091 DAOTAC010000000
VERSION     DAOTAC010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7999
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7694_length_7999_cov_5.177996"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..888)
                     /locus_tag="PLM74_05275"
                     /pseudo
     CDS             complement(76..888)
                     /locus_tag="PLM74_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298169.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(1068..2012)
                     /locus_tag="PLM74_05280"
     CDS             complement(1068..2012)
                     /locus_tag="PLM74_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HON42531.1"
                     /translation="MSGFADVVFNVNNLTATIRMATPIALAAMGGAFSERSGVVNIGL
                     EGMMLAGAFCGVLGSHLTGSPWLGVLLAVAGGGLVGAILAVFAIRLKADHVVTGVGIN
                     ILASGATTWLMQVIWKRKGATDMVAGLGTWSVPGLRDIPVLGTILGVHSPLVYISLIL
                     IVLGWVLMFRTPLGLRMRMTGEHPEAADTVGINVHAVQYLCVILSGCLAGLGGAYLSL
                     AQLNWFSKDMSAGRGYMALAACIFGGWNPLGGLGASFLFSFTDAIQMRMQTAQIPLPV
                     EIIQMIPYLLTILILAGAVVRSRPPAALGKHYEAGQVR"
     gene            complement(2009..3067)
                     /locus_tag="PLM74_05285"
     CDS             complement(2009..3067)
                     /locus_tag="PLM74_05285"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HON42532.1"
                     /translation="MGLRGNASRFAYSAIAVLLALLIGAVLIGASGYSVMEAYSNLLM
                     GAFGGVYSLATTVVMSTPLLFTGLAVAFAFQCGLFNVGAEGQLYVGALTSAVIALSFS
                     WLPPTLRVIVALAAGAGAGAALGFVPGYLKAKIGAHEVITTIMLNYVAISLTTFLVKN
                     YFRDPGPVDQTVLLPSGSRLPELVPGTRVSLGVVIGILAVALLDHVMRRSVLGYEIQA
                     VGRNPHAAEYGGVNVRARTVQAMMISGAVAGLAGSVEAMGTLYRFIPYFSPGYGFTGI
                     AVAVIAGQSMWAVIPAAVLFGALQSGGQSMQLFAGIPMELMTMVQGLVILFVAAPTLG
                     SMLLGLRRRLPGKGGGRA"
     gene            complement(3071..4585)
                     /locus_tag="PLM74_05290"
     CDS             complement(3071..4585)
                     /locus_tag="PLM74_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON42533.1"
                     /translation="MYAVRMENITKRFGNVAANDGVHFAALPGEIHGLLGENGAGKST
                     LMRILFGLYRADSGSIYVNDQPARITGPRDAISLGIGMVHQHFMLAEHLTVAENIVAG
                     CEPRRNGLLDYGRARRQICEVSDRYGLEVDPGARVEEISVGQQQRVEIIKALMRGAQT
                     LILDEPTAVLTPPEVDELFAVMRALREPGTTIVFITHKLKETMEVSDRVTVLRDGKTI
                     GTIDTSDATPDSLVEMMVGRSLPAHDDQRVPPREAVLLELRDVSVAGRKGSRARLDGV
                     SLAVRSGEVLGIAGVDGNGQLELEEVATGLRRPSKGRIVLIGQDVTGFDTGRIREKGV
                     ACIPSDRLKRGMVDSLSLARNSVLGVHRSSDFSRRGVLLENALREHAQGIVDKYDVRA
                     ESIDQTMGSLSGGNQQKMLVGRELGRPHSVVIAAQPTRGLDVGATHYIREQILASRAS
                     GKAVLLISAELDEVLELSDRIAVIYEGRIVDVRPTSSFTERELGLLMTGQRNEV"
     gene            complement(4719..5762)
                     /locus_tag="PLM74_05295"
     CDS             complement(4719..5762)
                     /locus_tag="PLM74_05295"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HON42534.1"
                     /translation="MTKTRKFSVLLGIALVAAMVMGMGAAGIAAAKPRMAIVFATGGL
                     GDKSFNDSAYDGMKRAEKELGIEFDKVEPQAVAEYESLLMRFAQTRKYDLIISIGFDQ
                     ADALQTVSARFPNQKFAIVDTVVDAPNVASYAYREAERGFVVGAVAGLMTTKKGDPRI
                     VADKQIVGVIGGMDIPLIRANVAGYIAGAKYVNPKVQVKYAYVGHWADPAKCKEMANA
                     MSDEGVDVLWLAAGRSGLGGIKAAEERNRYAIGADSDQGYLAPNNILTNGMKLVDNTV
                     YMAVDAVLKGKFTPGINLLGLSEGALGYSKNLVPADIIAQVDKLAAKVSSGELKVPDD
                     IDKVEPWLKSLGK"
     gene            complement(5813..6769)
                     /locus_tag="PLM74_05300"
     CDS             complement(5813..6769)
                     /locus_tag="PLM74_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HON42535.1"
                     /translation="MRALVFDDGLKAVDLPIPERDKGQALIRVDLAGICNTDVEVTRG
                     YLAYRGIMGHEFVGRVEDADNARLIGKRVVGEINITCGECGICRSGNPRHCERVKAVG
                     INGWQGCLADYVVLPERNLHIVPDSVTDRQAVFVEPLAAGLEILEQVHVRPADMVAVI
                     GDGKLGLLTAQAMYACGHRVIVLGKHVEKLAVTRRMGMDAAFASDYSGCVDVAVDCTG
                     NPSGMETAMSIVRPRGTIVLKSTFHEGSAMNLTPIVRNEITCVGSRCGPFPPALRLLK
                     TGAIAVEPLIDAIFSADEVIRAFERACARGSLKVLVDFGAGR"
     gene            complement(6885..7556)
                     /locus_tag="PLM74_05305"
     CDS             complement(6885..7556)
                     /locus_tag="PLM74_05305"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HON42536.1"
                     /translation="MNDQALREKVIQISRLMLSMGLTIGTSGNVSARCEDGESFLITP
                     SGMDYDAVTPDDIVMVNIATEERTGWRVPSIETGLHRGIYLARSDVGAVVHVHSPLAA
                     GLAVARKPLPVIADMCALAFGGQVEVAEYGTSGSPELAANVVKALGNRNAALMANHGS
                     LGVGKDLDQALLVCDLLEKMCLTYYCATLAGGAVPISSEAISILKNGVGKTYGQPGAA
                     TATAG"
BASE COUNT         1654 a         2623 c         2114 g         1608 t
ORIGIN      
        1 atggcaacgg tcgatgaagc aggaatcccg cgacttcagt cgtgggaggt tcaacagtgt
       61 gaagcttgta cttcaggcga tctccagcac cgccttaagg aactcaaggc tgtttaggaa
      121 cccctgctca tggcggtctt tcaccttttc gaggcgttcg cctagcgcca tgaacttggg
      181 gttacccagg tgtttcctga gacgtgcgat gaccctgatc tccacctctc gggctctctt
      241 ttcgtggtcg ggatcccgca gtatctcctc cagtacatca gcgtccatca caagcgtttc
      301 tacatcatca cgcactgact ccacgtacac gttctcattg ataagctgga ccgtcttggc
      361 cccgagggca tgccacagga gacgtccgct gccgctagga ggcttcaccg actcgtaaac
      421 ctgggtaagc cagcgatgat ctcgttcgta gggaccgaga acaggatcag gcgatagtgc
      481 ctcccagatg gcgctgagag ccgaatactc agctgcgaac ttatcgcgag catcattatt
      541 cggaagacac tgctgcgcgg ccataagtcc ttcgtagccg cccacgcttc tatcgactcc
      601 ggggaagaag gccaagcact tctgcacctg gaccggcagg gctttgcgca gctcgtcgat
      661 gttcgatact acctgctgca cagccttctc gtcgaagtcg agcgccctag cgacatcgtc
      721 aaaaatgcct atgtagtcca cgatcaggcc atgcgacttc ttctgaccga atgttcggtt
      781 cgtgcgacag atagcctgca gcaggttatg atccttcatc ggtttatcca agtacatcac
      841 ctgcaggatc tgcgcatcga agacggtcag gagtctgctc gtgactatgc cgaatagcac
      901 ttgcccaggt cggcagcacc gaaggaggat tcctcctccg gtcgtgacag tcgcggcgat
      961 gcaatggcaa cggcgctcga ggctattgcc cggcaagggg ggatctctgc gattcctgat
     1021 ccatgcacct ggcagcatga ggtgcgggag gaccgccggc gcagtcacta cctcacctgc
     1081 cccgcctcat agtgcttccc aagggccgcc ggcggtctcg atcggaccac tgcccccgca
     1141 agaatcagga tagtgaggag gtagggaatc atctgtatga tctctaccgg aagggggatc
     1201 tgagccgtct gcatacgcat ctgtatcgcg tcggtgaatg agaacagaaa acttgcaccc
     1261 aggcccccga ggggattcca gccgccgaat atgcatgccg ccagggccat gtatccgcgc
     1321 ccggcagaca tgtccttgct gaaccaattg agctgggcaa gcgatagata tgccccgccc
     1381 aacccggcga ggcacccgct gaggatcacg cagagatact gcactgcgtg cacgtttatt
     1441 cccacagtgt cggcggcttc gggatgctcg cctgtcatcc tcatgcgaag cccgagaggc
     1501 gtcctgaaca tcaacaccca gcccagaact atgagtatga gactgatgta gaccaagggc
     1561 gaatgcacac ccagaatggt cccaagcact ggtatgtcgc gcaaccccgg caccgaccac
     1621 gttccgaggc cggcgaccat gtcggtagcg cccttgcgct tccaaatcac ctgcatcagc
     1681 catgtggtgg cccccgaagc caggatgttt atgccgacgc ccgtgactac atggtcagcc
     1741 ttcagccgga tggcaaacac cgcaaggatg gcgccgacga gccctccgcc ggctaccgcc
     1801 agcagaaccc ccagccacgg gctgcccgtg aggtgcgatc ccaacacccc gcaaaacgct
     1861 cccgccagca tcatgccttc aagcccaata ttgactactc ccgacctttc ggagaatgcc
     1921 ccgcccatcg cagccagggc tataggcgta gccatcctga tagtggccgt gaggttgttt
     1981 acgttgaaga ctacatctgc aaacccgctc atgctcgccc tccgcctttc cccggcaagc
     2041 gacgccgcag cccaaggagc atgctcccaa gcgttggcgc agctacgaac agaatcacca
     2101 gcccttgcac catcgtcatc agctccatgg gtatcccggc gaagagctgc atagattgcc
     2161 cgcccgactg cagcgcccca aagagaacag ccgcagggat gaccgcccac atactctggc
     2221 ccgcgatcac cgccactgcg atcccggtga aaccatagcc gggtgagaag tagggtatga
     2281 accgatacag cgtacccatg gcctccaccg aacccgcaag gcccgcaact gcgccgctga
     2341 tcatcatggc ctgaacggtc ctggctcgca cattcacccc gccgtactcg gcagcatgcg
     2401 gattccgtcc aacggcttgt atctcgtagc cgagcacgct cctgcgcatc acatggtcaa
     2461 ggagcgcaac ggccaaaatt ccgatcacca cgcccagcga gacgcgggtt ccgggcacga
     2521 gctccggcaa cctggagccc gaaggaagca atacagtctg gtccaccggg cccggatccc
     2581 taaagtagtt cttcaccagg aaagtagtaa gagatatggc tacgtagttg agcatgatcg
     2641 tagtgatgac ttcgtgcgcc cctatcttgg ccttaaggta cccgggcaca aaacccaacg
     2701 cagcgccggc ccctgccccc gctgcgagcg cgacgataac tcgaagcgtc ggcggcagcc
     2761 aggagaacga cagagcaatc acggcagatg tcaatgcacc cacgtatagt tgcccctcag
     2821 cccccacgtt gaacaagccg cactggaatg cgaatgccac tgcaagcccg gtgaatagta
     2881 gaggtgtcga catgaccaca gtggttgcca ggctgtacac gcctccgaaa gcccccataa
     2941 ggaggttgga gtatgcttcc atgacgctgt agccggaagc ccctatcaga acagcgccaa
     3001 tcaacaacgc cagcagcact gcgatggctg agtatgcaaa ccttgatgca ttccctcgga
     3061 ggcccatcgg tcacacctcg ttcctctgcc cggtcatcag cagacccagc tcacgctcgg
     3121 tgaagctgct cgtcggacgc acgtccacta ttcggccttc atagataacc gcgatcctgt
     3181 cagacaactc aagcacctca tctagctccg ccgatatgag gaggactgcc ttgccgctag
     3241 cgcgactagc aaggatctgc tctctgatgt aatgggtcgc cccgacatcc aacccgcgcg
     3301 tgggctgcgc tgcaatcaca acactgtggg gacggcccag ctcgcggcct accagcatct
     3361 tctgctggtt cccccctgac agcgaaccca tagtctgatc gatggactcg gcgcgtacat
     3421 cgtacttgtc gacgatcccc tgggcgtgtt cacggagtgc attttcgagg agcactccgc
     3481 gtcggctaaa gtccgagctg cgatggacgc caagaactga gtttcgagcc aggctcaacg
     3541 aatcaaccat gcctcgcttc agtctgtcgg aagggatgca ggcaacgcct ttctccctga
     3601 tcctgccggt gtcaaagccg gtcacatcct gaccgatcag cacgatcctt cctttgctcg
     3661 gcctgcggag cccagtcgcc acttcctcca gttccagctg gccgttgccg tcaacaccgg
     3721 ctatgcccaa cacctcgccg ctcctcacag ccaggctcac accgtccaat cgtgcacgac
     3781 tgcccttcct tccggctacg gagacatccc taagttcaag caagacagcc tcgcgcggcg
     3841 gcactcgctg atcatcgtga gcaggaagac tccgtcccac catcatctca accagactgt
     3901 caggcgtggc gtcggacgta tcgatggtgc caatggtctt tccgtcgcgc agtacagtca
     3961 ctctatctga gacttccata gtctccttga gcttgtgcgt aatgaagact atggtggttc
     4021 cgggttcgcg gagagcccgc atgacggcaa acagctcatc cacctcagga ggtgtaagca
     4081 ccgcggtcgg ctcgtccaag ataagtgttt gcgcgcctct catgagagcc ttaattatct
     4141 ccaccctttg ctgctgccca acggatattt cctccacccg agcgccagga tctacttcaa
     4201 ggccgtagcg gtccgacacc tcgcagatct gcctgcgagc ccgaccgtag tcgagcagac
     4261 catttcttcg cggttcgcat ccggcaacta tgttttcggc gacggtgagg tgttcagcca
     4321 gcatgaagtg ttgatgcacc atacctatgc ccagcgaaat ggcatcacga ggtcctgtaa
     4381 tgcgcgccgg ctgatcgttt acgtatatgg agccggagtc ggcgcggtag agcccgaaca
     4441 agatccgcat aagtgtgctc ttccccgccc cgttctcccc gagaagaccg tggatttcgc
     4501 cgggaagcgc ggcaaaatgc acgccatcgt tggccgcgac gtttccaaag cgtttcgtga
     4561 tattctccat tcgaacggca tacatcgctc ggcgaacccc ttttccgcgg catacctggc
     4621 agggcagggg gagtgaatcc ccccgcccgc agcgtcaagc cattgctgtc acgcgctcaa
     4681 gctcgctatc cccctagagc ctgtgcggca gccgctggtt acttgcccag agacttcagc
     4741 cagggctcaa ccttgtcgat gtcgtccggc accttgagct cgccggacga aaccttggct
     4801 gccagcttgt cgacttgggc gattatgtcg gcaggcacta ggttcttgga ataaccgagc
     4861 gccccttcgg acagacccag caggttgatg ccgggcgtga acttgccttt cagcacggcg
     4921 tcaacggcca tatacacggt gttgtccaca agcttcattc cattggtcag tatgttgttg
     4981 ggcgcaagat agccctggtc ggagtcggcg ccgatagcgt agcggttgcg ctcctcagct
     5041 gccttgatac cgccgagccc cgatcggccg gctgcgagcc acagcacatc aacaccctca
     5101 tccgacatcg cgttggccat ctccttgcac ttggccggat cggcccagtg gccaacatac
     5161 gcgtatttca cctggacctt ggggttaacg tacttggccc ccgctatgta ccctgcgacg
     5221 ttggcgcgga tgagcggtat gtccatgccg ccgataacac cgactatctg cttgtcggca
     5281 acgatcctgg gatcgccctt cttggtggtc ataagccctg ctacggcgcc tacaacgaac
     5341 ccgcgttcag cctctctgta cgcgtatgac gccacgttgg gagcgtcgac cactgtgtcc
     5401 acgatggcga acttctgatt ggggaacctg gcagacacag tctgcagcgc gtctgcctgg
     5461 tcgaaaccga tggatatgat gaggtcgtac ttgcgagtct gggcaaaacg catcagcagg
     5521 ctctcgtact ccgccacagc ctggggttca accttgtcga actctattcc gagttccttc
     5581 tccgcacgct tcatgccgtc ataggcagaa tcgttgaacg acttgtcgcc aagaccaccc
     5641 gtggcgaaca caatcgccat tctgggctta gctgcggcga tgcccgccgc gcccatcccc
     5701 atcaccatag ccgcaaccaa tgcgatgccg agcaacactg aaaacttccg agtttttgtc
     5761 atctctcaga tcccctcctc cagatttgtg gctgtgtgac atgccctggc gctcatctcc
     5821 cggcgccgaa atcgaccaac accttcaggc tccctcgggc gcatgctctc tcgaacgcgc
     5881 gtatcacctc atcagctgag aaaatcgcat caatcagcgg ctctaccgcg atcgcccccg
     5941 tcttgagcag tctcagcgcc ggaggaaatg gtccacaccg cgagcccaca caggtgatct
     6001 cgttacgtac tattggagta aggttcatag cagagccttc gtggaaggtg ctcttcagca
     6061 caatcgttcc cctggggcgc acgatggaca ttgccgtttc cattcctgac gggttgcctg
     6121 tgcagtccac agccacgtcg acgcagcccg aatagtccga tgcaaacgcc gcatccatgc
     6181 ccatcctcct cgtaaccgcc agcttctcca cgtgcttccc cagtacgatg acgcggtgac
     6241 cacaggcgta catagcttga gctgtgagaa gccccaattt gccgtcgcct atcaccgcaa
     6301 ccatgtcggc aggcctgaca tgcacctgct ccaggatttc gagcccggct gcgaggggtt
     6361 ccacaaacac cgcctgacgg tcagtgaccg agtctgggac gatgtgaaga ttgcgttcgg
     6421 gcagaactac gtaatcggca aggcagccct gccagccgtt tatacccacg gctttgacgc
     6481 gctcgcaatg acggggattg ccgctccggc aaattccgca ctcgccgcag gttatgttga
     6541 tctctcccac gacgcgtttc ccaatcaggc gcgcgttgtc ggcgtcttct accctgccca
     6601 cgaactcatg gcccatgatc cctctatagg ccagatagcc ccgggtcacc tcaacatcgg
     6661 tattgcagat ccccgcaagg tctacacgta tcagcgcctg ccccttatcc ctttccggga
     6721 taggcaagtc caccgctttc aggccatcgt cgaaaaccaa cgccctcacc ggaggcattc
     6781 ctccttccgc tctccattcg ccaatccaca tccgccccac gaaagcccgc ccgcgcccgg
     6841 ttgcgttgcc ttgcggcctt agcgccgcgc gaccggccgg cgacttatcc ggccgtggcg
     6901 gtggcggccc ctggctgccc gtacgtcttg ccgacgccgt ttttcaggat gctgatcgcc
     6961 tcactagaaa tcggaacggc cccgccagcc aaagtagcac agtaataggt aaggcacatc
     7021 ttctcgagca gatcgcagac tagcagcgcc tgatcaagat cctttccaac gccgagcgat
     7081 ccgtggtttg ccatcagagc ggcgtttcga ttcccgaggg ccttcaccac attggcggca
     7141 agctccgggc tgcccgaagt gccatactcg gccacctcga cctgcccgcc gaaggccagg
     7201 gcgcacatgt cggctatcac cggcagtggc tttctcgcaa cagcgagccc cgcagccaag
     7261 ggcgaatgaa catgaacaac cgcgcctaca tctgaacgag cgaggtagat cccgcggtga
     7321 aggccggtct cgatagacgg aacccgccat cccgtcctct cttcggtcgc tatgttcacc
     7381 atcacaatat cgtcgggcgt gaccgcatca taatccatgc ctgatggcgt aatcagaaag
     7441 ctctcgccat cctcgcaccg agcgctcacg tttccgcttg tgccaatagt cagccccatg
     7501 gatagcatga ggcggcttat ctgaatgacc ttctcccgca aagcctggtc gttcatacac
     7561 cgtgcccctc tcaacccgaa ccgagatgat cgtcataact tgtattctct gcctaacgct
     7621 cgtatcctcc actcgaacca cacgccatcc actcttgtca cccacccgcg ctacttccgg
     7681 tgcgtgtaag gattgtggct ttcggggtcg cagatacccc gcattcaggc gtgcagtccc
     7741 gccagatcca cagctgtggc tcagtgatat acccccgggg gtatccgccc agagatacgg
     7801 tcagattttg ataatagttt tggcgaaaag cgcagcttct gagtacatcg atttgggccg
     7861 ggggccctag gggggcaaat cggccctcca aggggagccc ccaaggctga ttgccgccta
     7921 gttattggtg aatctctggc gctgcatgac atagctcgcg agctgctgcc aaacaacgcc
     7981 atatcgtggg atattatgt
//
LOCUS       DAOTAC010000092         7985 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_7711_length_7985_cov_5.234426, whole genome shotgun sequence.
ACCESSION   DAOTAC010000092 DAOTAC010000000
VERSION     DAOTAC010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7985
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7711_length_7985_cov_5.234426"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..959)
                     /locus_tag="PLM74_05310"
     CDS             complement(<1..959)
                     /locus_tag="PLM74_05310"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HON42537.1"
                     /translation="MPSSTEPSLIHEIMTGTFTAEQLTAGTSAVISSLIRIGIIVLVS
                     VLVMRVASRLIDRLFKSREDEQRVLIRDNRRLMTLGTVIKSFVRYMVYFVGGIMVLGE
                     LGVNTSGLVAGAGIAGVALGFGAQNLVRDVLTGFFILFEDQFAVGDYVSISGVSGVVE
                     EIGLRVTKVRDFAGQLHVVPNGSISLVTNFRGRAMRVMFDVQVPYSTNVELAIGVLQD
                     AFDRHRESIPWIVDGPTVLGVQELGDSGIALRVWATAEPMQQWSLERQLKLLVKRVLD
                     DNGISVAYPHRYVIIDGQPRSVDSGQLHSADCGIPETEPWSDRA"
     gene            complement(1099..1434)
                     /locus_tag="PLM74_05315"
     CDS             complement(1099..1434)
                     /locus_tag="PLM74_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HON42538.1"
                     /translation="MGQWLKHLVRLVVSAIVLMFISFLIPGFATVTFWTALLAAAVIT
                     VLGWLIEVILGRAVSPYARGIVGFLVSAVVIYATKFIVPGMTVSIAGALLAAFVIGLV
                     DMFVPTTLR"
     gene            complement(1559..3067)
                     /locus_tag="PLM74_05320"
     CDS             complement(1559..3067)
                     /locus_tag="PLM74_05320"
                     /inference="COORDINATES: protein motif:HMM:NF019924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HON42539.1"
                     /translation="MKGGERRLDVSGITRGACRQVSMIISLATFEHLPKRVGGLAEAA
                     TSIGESLARDNEVMVFMPSHGIQKTEKEFRVKKYGKFKITAGPSPFEVTVYEMDRNGV
                     RVFLLSDDVMDHPDVYFPRETLTAKLVHFAHAFPAAINLIMGREGKKPDVAHINDWHC
                     VLGGAVARKYLRIPMVYTIHRICREKIAVDEMERAGLREFIDPECIEVNKEGIMLNLE
                     TFGCRVCDYLTTVSYSYLNEEWKTLFGEYAGKATYVWNGMDFSFWDPSRLTDAGKPRD
                     ERRAAILQEQGLDDGILYFYVGRFDMEQKGVDHMFAGAQMVLEGKLPGAERLAPLFRM
                     VVLGSGDPVLEDMARDMERKYPRNIKAIIGYLGREATRAWYGASDFCLIPSNFEPFGL
                     VQLEAMCMGSIPIGTKVGGINDTVLDIDKAKEGATGKLVPPRNPRELAGAMVKMGLVY
                     MDDRARLEQVRSNGRPHVMTNFTWDRAARRYLSVYEGTASVKLPFADYGGPF"
     gene            complement(3428..3949)
                     /locus_tag="PLM74_05325"
     CDS             complement(3428..3949)
                     /locus_tag="PLM74_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HON42540.1"
                     /translation="MILLNLYLSFAKVGLFTFGGGYAMLPLIESEIIHGRGWLTVREF
                     TDIVAIAEVTPGPIAVNSATFVGYRLAGVLGGIVATLGVVTPSVVIILALAMLAKRFE
                     NSTGLARVKSGMRPAVVGLIAAAALTVARVALVDLKSIMMAAMAALLVFKVKLDPILV
                     LAISGLLGAIIFS"
     gene            complement(3946..4566)
                     /locus_tag="PLM74_05330"
     CDS             complement(3946..4566)
                     /locus_tag="PLM74_05330"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HON42541.1"
                     /translation="MDSRFATDSEPSENHRSETGGPARASVAALFWTFFRIGAFTIGG
                     GYVMVPLIQRDIVERRGWIDRDSFVDVLAIAQTAPGPLAVNTAVYVGYMLRGIWGAMA
                     SLAGCILPSIIIILVVAALFDQISSSSYVQAAFAGIRPAVVVLIVSAAIKIGRPVVRS
                     RVELALAAMAMVLVAFVRISPALVVTAAAIYGLLSRSISGNGGKDA"
     gene            complement(4600..4806)
                     /locus_tag="PLM74_05335"
     CDS             complement(4600..4806)
                     /locus_tag="PLM74_05335"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HON42542.1"
                     /translation="MGYVWMAVEFASFIAMGFVIGNYADSRYGTRPWGALAGFMIGLV
                     GGALSVNRAVSELSTRERSRRKKR"
     gene            complement(4846..6231)
                     /locus_tag="PLM74_05340"
     CDS             complement(4846..6231)
                     /locus_tag="PLM74_05340"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HON42543.1"
                     /translation="MGEISNPKLRRAVMAVGIVACLIVGALWSTGIFAPGVLEGNRIS
                     RGVTVGGHDVGGMTVSEARMLLQTQMSKCETAPISLRLGGETWRVSPEEIGTVVDVEP
                     ALKAAYSIGRSGRLYARLAQRRQARREGVDIPLVVSVDEQRLRDLVFALASVVQVQPV
                     DARIVITDGDEVIIEPSSEGRRLNVDELISRLRSTTLERGAREIDIPVETAPPAVVTS
                     ELESRGIARLLGEYTTKFKASNVKRSENIRLGAAMIDGTIVAPGDVFSFNEVVGPRTP
                     ERGFLEADIILNAELVPGIGGGICQVSTTLYNAALLSDLEIMSRINHSLPISYVPLGR
                     DATVSYGAIDLKIRNNTGHHVLLKARVDKDTITFKIYGDLPRDMIVGIETQVLEKIEP
                     GVIEQVDAKSPPGSRTTIQTGAPGYLVAVWRVVKSEGVEIRRELISRDRYKPQPVIVK
                     AGPSPQAVDTP"
     gene            complement(6242..6727)
                     /locus_tag="PLM74_05345"
     CDS             complement(6242..6727)
                     /locus_tag="PLM74_05345"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HON42544.1"
                     /translation="MTSQEKNCRMAHSHNCTGMCYARLGDHAEARREFEAAISLDHRF
                     ADAHCNLGNTLREPGDLDGAVASYHDALEIEPERLDARLNLISTLRERHRVAGPAFAT
                     GAMVGDHAFGDCEAHGRVPRSLRKPRVDDHSFRRGVIVLGYCLVAVIMCVTFVLWILR
                     R"
     gene            6977..7471
                     /gene="yyaC"
                     /locus_tag="PLM74_05350"
     CDS             6977..7471
                     /gene="yyaC"
                     /locus_tag="PLM74_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013524884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HON42545.1"
                     /translation="MAASLSEPLGARCQPLTVVCIGTDRSTGDALGPLVGTRLAEWRC
                     ARDHIRVVGTLDEPVHAGNLRGAIEALGLAGPRAGHVLAVDACLGRVGSIGYITVGEG
                     PLKPGAGVNKELPPIGDAYITGVVNVGGFMEYLVLQNTRLSLVIKMADAIARGVVTYV
                     AGPA"
     gene            complement(7513..>7985)
                     /locus_tag="PLM74_05355"
     CDS             complement(7513..>7985)
                     /locus_tag="PLM74_05355"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HON42546.1"
                     /translation="SPFNPEMGIGAVGPDGNVVLDQLLISRLKVDAEYIENVSRLQLD
                     ETRRRLSAYRGARPSPKVSGRDVIVVDDGIATGYTVMAAVRSLRGSDPLRLVVATPVC
                     PPDTREWLQQEVDMVVSLITPEEFAAVGQFYVDFTQVSDAEVIEILSKRPVQET"
BASE COUNT         1680 a         2525 c         2122 g         1658 t
ORIGIN      
        1 gctcggtcag accacggctc agtctcgggg attccgcagt ccgcgctgtg cagctgcccg
       61 ctgtccacgc tgcgcggctg tccatcgatg ataacgtacc tgtgcggata cgcaacgctg
      121 ataccgttgt catcgagtac ccgcttgaca agcaacttca gctgccgctc cagagaccat
      181 tgctgcattg gctcggcggt cgcccatacc ctcagagcga ttcccgagtc gcctaattct
      241 tgaaccccta ggacggtggg accgtccact atccagggta tactttccct gtggcggtca
      301 aaagcgtcct gcagtacccc gatggcgagt tctacgttag tcgaataggg cacctgtaca
      361 tcgaacatga ctctcattgc acggccccgg aagttggtaa ccaggctaat cgatccgttg
      421 ggcacgacgt ggagttgccc ggcgaaatcc cggaccttgg tgaccctcag tccaatctcc
      481 tcgactactc ccgacacacc cgaaatcgag acgtagtcgc ccacggcaaa ctgatcttcg
      541 aatagtatga aaaagccggt taggacatcg cgtaccaggt tctgcgcccc aaaacccagg
      601 gcaactccag ctatcccggc gcctgccaca agaccggacg tgttgacccc cagttcgccg
      661 aggaccatga tcccgcccac gaagtacacc atatacctga caaagctctt tatcacagtt
      721 cccagagtca tcaagcgcct gttgtcgcga atcagcacgc gctgctcatc ctcgcgtgat
      781 ttgaacaggc ggtctatgag cctggaggca acgcgcatta ccaagaccga tacaagtacg
      841 attatcccaa tcctgatcaa tgaggagatc accgcactcg tgcccgcggt gagctgctcg
      901 gccgtgaatg tgcccgtcat aatctcgtgt atcaacgaag gctctgttga gctcggcatg
      961 tgccaccacc tccgaagaat ccgctcctca gtacctttag acatccttca gtcatgtcct
     1021 gctctaaagg gaaaaacccc tctcgggcag ccgccgaaag gggtgcgcgc gaaggttgat
     1081 tgtttcacat gaaacatttc acctcagggt cgtgggcacg aacatatcca ccagcccgat
     1141 gacgaaagct gccaacaacg cgcctgctat gctgaccgtc atgcccggca cgatgaactt
     1201 ggtggcgtat atcaccaccg ccgacaccag gaaacccaca atccctctgg catagggaga
     1261 gaccgcgcgc cccaggatga cctcgattag ccaccccaaa acagtgatca ctgctgcggc
     1321 aagcagcgca gtccagaacg tgaccgtcgc aaaccccggt atgagaaacg agatgaacat
     1381 cagcacaatc gctgacacaa ccagtctcac aaggtgcttt agccactgac ccatcgactc
     1441 tgccccccag tacatgtgct ggtttagttc gccaatcgtg gtattagcat aaccagcatc
     1501 aaggggacta tgcttgcaag gacttgcggg ctgtaccgat cgattgagcc tcggccttct
     1561 agaatggtcc tccgtaatcg gcaaacggca gtttcaccga agcagtaccc tcatatacag
     1621 ataggtacct gcgtgctgcc cgatcccagg tgaagtttgt cataacgtgg ggtctcccgt
     1681 tggacctaac ctgctcaagt ctggccctgt catccatgta cacaagaccc atcttgacca
     1741 tggctcccgc cagttcgcgc ggattgcgag gaggcacgag cttcccggtg gcgccttcct
     1801 tggccttgtc gatatccaat actgtatcgt ttatgccgcc gactttggtt ccaatcggta
     1861 tggaccccat gcacatcgct tccagctgta ccagcccgaa tggctcaaag ttggagggta
     1921 tcaagcagaa gtcggacgcg ccgtaccatg cacgggttgc ctccctgccc agatagccta
     1981 taatggcctt gatgttcctg gggtacttgc gttccatatc ccgcgccatg tcctcaagaa
     2041 cggggtcgcc cgaacccagc acgaccatcc ggaaaagcgg agccaatctt tctgcgcccg
     2101 gcaatttccc ttctaggacc atctgggcgc cagcaaacat gtgatctacg cctttctgct
     2161 ccatatcgaa gcgcccgacg tagaagtaga gaatgccgtc atctagtccc tgttcttgca
     2221 ggatggcggc gcgtcgctcg tccctgggtt tgcccgcgtc tgtcaaccgg gaaggatccc
     2281 agaaggaaaa gtccataccg ttccacacgt aggttgcctt gcccgcatac tctccaaaga
     2341 gagtcttcca ctcctcgttc aggtaactgt agctgaccgt tgtcaagtag tcacatacgc
     2401 ggcatccgaa tgtctcgagg ttaagcatga taccttcctt attcacctca atgcactcag
     2461 ggtcgatgaa ctcccggagg ccggcccgct ccatttcgtc cacggcgatc ttctcccggc
     2521 atatacgatg gatcgtatat accatgggta tccgaaggta cttccgcgca accgcgcctc
     2581 cgaggacaca gtgccagtcg ttgatatgag caacatcggg tttcttgccc tctctgccca
     2641 tgatcaggtt gattgcagcc ggaaaggcgt gagcgaaatg aactaacttg gccgtgagcg
     2701 tctcgcgagg gaagtacacg tcggggtgat ccatgacatc gtcgctcagc aggaaaaccc
     2761 gaacgccgtt gcgatccatc tcatacacgg tgacttcgaa ggggctaggc ccggcggtga
     2821 tcttgaactt gccgtatttc tttaccctga actctttctc ggtcttctga atgccgtgag
     2881 acggcatgaa aaccatcact tcattgtcac gcgcgagcga ctcgcctatc gaggtcgcgg
     2941 cctcggccag tcctcccact cgtttgggga ggtgttcgaa ggttgccagg ctgattatca
     3001 tgctaacctg gcggcatgcg ccccgggtta ttcccgagac atcaagccgc cgctcgcctc
     3061 ctttcatgcg ctgctctgtt cgtcctgccc gagcccagag tcaacataac atagtgtgcc
     3121 gctgttgacg gccaaaactc acgcatcgtc ggacatcaat tgcagaatac caaagggtgg
     3181 acaagcgggc aaggtcttgc ctgcggcagt tacagtatat gacatctacg ccgtcagaat
     3241 tgcttgcttg tgaagcgtgc ggcgcggcag gcaactgtca cccggcaacc aacgacaata
     3301 taatggagtg ccgccgtacg gctgcactcc cgacacgagg agggatgtca gatgtggcga
     3361 ttccttgaaa ttgcgatcag cccgtttttc aggtggcgcg gccgaacctg catcctcata
     3421 gtgcggacta cgagaagatg atggcgccca gaagccccga tatggccaac accaggatgg
     3481 ggtcgagttt caccttgaat acaagcagag ctgccattgc agccatcatt atcgacttca
     3541 ggtccacaag ggccacgcgc gccacggtca gcgccgccgc agcgattaat cctacaacgg
     3601 ccggccgcat gcccgacttc acccgcgcca gcccggtgct gttctcgaac cgcttggcca
     3661 acatggccag cgccaggatg ataacaactg atggagtcac cactccgagc gtggccacga
     3721 ttcctcctaa taccccggca agccgatagc caacaaaagt cgcgctgttg acggcgattg
     3781 gccccggggt cacctctgca atagccacga tgtcggtgaa ctcccgaacg gtgagccacc
     3841 ctcgtccatg gattatctcg ctctctatca gcggtaacat ggcataaccg cccccgaacg
     3901 tgaacagccc gactttggcg aaactcagat agaggttgag caggatcatg cgtcttttcc
     3961 cccattgccc gagatactgc gtgacaagag tccgtagatc gcagcagccg tcacaaccag
     4021 cgccgggctg atgcgcacga atgcgaccaa aaccatggcc atggcggcca gcgccaattc
     4081 cactcgggag cgcaccactg gcctgccgat cttgattgct gcagatacga tcaacacaac
     4141 gacagccggc cggatccctg caaacgcggc ctgtacgtaa ctggagctgg atatctggtc
     4201 gaagagcgca gctactacca ggatgatgat gattgacgga agaatgcagc cggcaagcga
     4261 tgccatggcg ccccaaatcc cgcggagcat gtacccaacg tacaccgcgg tgttcacagc
     4321 taagggaccg ggagcagtct gggcgatcgc gaggacatcc acgaaactgt ccctatcgat
     4381 ccaccctcga cgttccacga tgtcgcgttg gatcagcggc accattacat atcccccgcc
     4441 gatggtgaat gcgcctatcc tgaagaatgt ccagaacaga gcggcgactg aagctctcgc
     4501 gggcccacca gtctcggatc tgtggttttc actgggctcg gagtccgtag cgaatctaga
     4561 atccattgac ctgtctcctt caacgatgat gctgcggggt cagcgcttct tcctcctgct
     4621 gcgttctctt gttgaaagtt cgctcactgc ccgattgacg ctgagcgcgc cgccgaccag
     4681 acctatcatg aatcccgcca gtgcccccca tggtcttgtc ccataccggg agtcggcgta
     4741 gttgccgata acgaagccca tggctatgaa actcgcaaac tccaccgcca tccacacata
     4801 gcccatatgg actcagctcc ccgcagttct cggatcagcc ggacatcatg gggtatccac
     4861 agcctgcggt gatggtcccg ctttcactat caccggctgg ggcttgtacc tgtcccgcga
     4921 aatcagctct cgcctgattt ccacgccttc cgatttcact acacgccaca cagccaccag
     4981 atacccgggc gcgccggtct gaatcgtcgt ccgcgaacca ggcggtgatt tcgcatctac
     5041 ctgctcgatc accccgggtt cgatcttctc gagcacctgg gtctcgatac ctactatcat
     5101 gtctcgtggg aggtcgccgt aaattttgaa cgtgatggta tccttgtcga cgcgggcctt
     5161 caggagcaca tgatggcccg tgttgttcct tatcttgagg tcaatggcgc cgtacgacac
     5221 cgtagcgtcc cttcccaaag gtacgtagct gatgggaagc gagtggttaa tcctcgacat
     5281 gatctccaag tcggacagca gagcagcatt gtaaagagtc gtggacacct gacatattcc
     5341 gccgcctatt cccggcacga gttcggcgtt gagtatgatg tcggcttcca ggaagcctct
     5401 ctctggggtt cgcgggccta ccacttcgtt aaaagagaag acgtctcccg gcgcaacgat
     5461 agtaccatca atcatcgccg cacccagccg gatgttctct gaccgcttga cattgctcgc
     5521 cttgaatttc gtggtgtact cgcccagcag acgtgcaatc cccctcgatt ccagctccga
     5581 cgtgaccaca gcgggcggag cggtttccac aggtatatcg atctcgcgcg ctccgcgctc
     5641 cagcgtcgta cttctcaacc tggagattag ctcatccaca ttgagccggc gtccttcgga
     5701 cgagggctca atgatgacct catcgccgtc tgttatgaca atcctggcgt caaccggttg
     5761 gacctgcact acgctggcga gagcaaagac caggtctcgc aggcgctgct cgtcgacaga
     5821 aaccaccaga ggtatgtcga cgccctcgcg cctcgcctgc cggcgctggg ccaatcttgc
     5881 gtacagccga ccactgcggc ctatgctgta cgcagccttc aaggctggtt ccacatcgac
     5941 aacggtaccg atctcttcgg ggctgactcg ccatgtctca ccacccaatc tcagagagat
     6001 gggcgctgtc tcgcacttgc tcatctgggt ctgcaggagc atcctggcct cggatacagt
     6061 catcccgccc acatcgtggc ctcccacggt gaccccacgc gagattctat tgccttccag
     6121 cacaccgggg gcaaatatcc ctgtgctcca caatgcgcca acgatcagac atgcgactat
     6181 gcccacagcc atgactgctc tgcgcagctt ggggttactg atctctccca taggcggcaa
     6241 actacctccg caatatccat aacacaaagg ttacgcacat tatgacagcc accagacagt
     6301 agcctaacac gatcactccc cgccgaaagg agtgatcgtc gacgcgtggt ttccgaagcg
     6361 accgcggaac tcgcccgtgc gcctcgcagt cgccgaacgc atgatcgccc accatggcgc
     6421 ctgttgcgaa cgctggtccc gcgactctgt gtcgttcccg cagtgtggag atgaggttca
     6481 gcctggcatc gagacgctcg ggttcgattt caagcgcatc gtggtacgat gctaccgcac
     6541 cgtccaagtc tcccggttcc ctcagggtgt tgcccaggtt acagtgggcg tcagcaaacc
     6601 tgtgatcaag cgaaatcgcg gcctcaaact cgcgccgggc ctctgcatgg tcgcccagac
     6661 gagcatagca cattcctgtg cagttgtgcg aatgcgccat ccggcaattc ttctcctggc
     6721 tggtcaactg actcgccctc ctgccgattg agcatcgtca tctcgggccc acatagaaga
     6781 cggtgtgtgc tcatatactt caactagcac gtccggtgga tatcgccgac gtctttacag
     6841 tcagaggagg tcgctcgtgg aaagcaagcc tgatgctacc tatgagaacc gccccgccat
     6901 cctcaggagt ccttcgtccg gacccacccg ggctcatgtc gacagcccca acttcagctc
     6961 ctggcttgcg cgcgagatcg ctgccagtct cagcgagccg cttggggcgc ggtgccagcc
     7021 gctcaccgta gtgtgtatcg gcactgaccg atccacaggc gatgcactcg gcccactggt
     7081 cggcaccaga ctggcggaat ggcgatgtgc acgcgaccat atccgtgtag ttggcactct
     7141 agatgaaccg gtgcatgccg gcaatcttcg cggcgccatc gaagcgcttg ggctcgccgg
     7201 gcctcgggcg gggcatgtgc tcgccgtcga cgcctgcctg ggccgcgtgg gcagcatcgg
     7261 ctacatcacc gtaggcgaag ggccgctgaa gcccggcgcc ggcgtcaaca aggagttacc
     7321 tccgatcggt gatgcctata tcactggagt agtaaacgtg ggcgggttca tggagtatct
     7381 cgtgcttcag aatacgcgcc tatcgctggt gatcaagatg gcagatgcca tcgcccgcgg
     7441 agtcgtcaca tatgtggccg gtccagccta ggatggcgcc tgaacaccgg agagggtggt
     7501 cggaaggcac acttaggtct cctgcaccgg ccgcttcgac aggatctcga ttacttcagc
     7561 atccgatacc tgggtaaagt ccacatagaa ctggcccacc gcggcaaatt cctccggagt
     7621 tatcaggctc actaccatgt cgacctcctg ctgcagccat tcgcgagtat ctggagggca
     7681 tactggcgtt gcgactacca gccggagcgg atcgctgccg cggaggctgc gcactgcggc
     7741 catcactgtg tagccggttg cgattccgtc gtccacaacg attacgtccc tgccggagac
     7801 ctttggagat ggtcgcgccc cccggtatgc cgacagccgt ctgcgagtct cgtccagctg
     7861 aaggcggctt acgttctcga tgtactcggc gtccactttc agccgactga tgaggagctg
     7921 atccaggact acgttgccgt cgggcccaac cgcgcctatg cccatttccg ggttgaaagg
     7981 cgaac
//
LOCUS       DAOTAC010000093         7968 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_7729_length_7968_cov_5.090105, whole genome shotgun sequence.
ACCESSION   DAOTAC010000093 DAOTAC010000000
VERSION     DAOTAC010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7968
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7729_length_7968_cov_5.090105"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1345..2130
                     /locus_tag="PLM74_05360"
     CDS             1345..2130
                     /locus_tag="PLM74_05360"
                     /inference="COORDINATES: protein motif:HMM:NF014759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T7 exonuclease"
                     /protein_id="HON42547.1"
                     /translation="MDLLIDADILACQFAYAYEARRGGGGVRAAVAVDGDMDGEARDD
                     GLRTDGRWAIAGFNSAVERMLRETRTDKAILCFTGCENFRYAILPTYKANRRGTPRPV
                     LLDVLIEHARQAWECRSEDSLEADDVMGIMATRSPRNYVMASTDKDLKQVPGVHYNWR
                     TDCMERVTTPDADYWFYFQVLTGDSTDGYSGCPGVGPRRAKTVLEGAGAHPWASIVAA
                     FESKGLTEEDALAQARVARILRCDDYDFVRRRPIMWRPRLDWT"
     gene            2147..4579
                     /locus_tag="PLM74_05365"
     CDS             2147..4579
                     /locus_tag="PLM74_05365"
                     /inference="COORDINATES: protein motif:HMM:NF021235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HON42548.1"
                     /translation="MVARRRRSTKEMTQSRQTRRVWQRIGLAAVVLIVAVAASGYYLI
                     YKSMAELPAEGAIAGRVTATTGFIPYSMPGIAGVTVAAETLDSRLDAEDASGTLDAAG
                     QAEGPVSSPGRYTAKTNGQGVFSLDVPPGVYVVTFEKRGFETYSERVIAMPSGQAPVF
                     VSLFREPRGKAVARLKGPKASKGPVPYHTSVRIDATGSKNVSRYGVRWEVRDATGSVL
                     MDPYASTAEPLQLEPSPIPGSSALDFRFVPLEPGEYTVTVFMRNQLSDEESSASVTLI
                     AANTAPTAIANVMAGPGRPSKTPSGALKRSPGLATVVVGAPVFLEGWGVDVNAPSPET
                     YNPGGRIADAYGKNDDWDQRQFAWDWRLEYVSPDGLRSGVTDALLSTEDVEADATDGA
                     NTAKGLQRPWFVADRVGEYVAYVTVSDRDPFGEPLSGSASVRVRVVEPEAVAASEAAC
                     VECHKAQANPVGNMSCQWCHGPAEPHLAAKGDREKRETMEVSYDSGLCGQCHAQYAEW
                     EKSRHSDGYPFGYHEIARPLLLNCAKCHYPEGFVEAADRADKHGMSFGEVQFKRPMFA
                     GGPLFFDFSQLPPEQGEGIACLSCHDPHPGDGAGACSAGLRVNKSELCGTCHEEKWQN
                     VLLNGTAGQVGSAFEYQGTDYRVANPHDTEEKCVLCHMNDAEVGAAATDSAGVAELGG
                     HTMRMRAGASGGDVLNLEPCRTCHSDADTFDINGRQSEIRALCEELGSLLRARNNGVL
                     PGYRPGDKCATCHRGGTLPFDDDPRLVLENAYTNYKLVMNDRSWGVHNYEYTRRLLED
                     SIESVRSISPGQ"
     gene            complement(4563..4934)
                     /locus_tag="PLM74_05370"
     CDS             complement(4563..4934)
                     /locus_tag="PLM74_05370"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HON42549.1"
                     /translation="MRPAGFGSWQAAAALVFGAIAKELIVGTLGVLYGSGVASGVASG
                     SGAGEIGLIGAIQANWTPLSAYAFLVMSLVYMPCVATIAAIRRETGSRGWTAFAMAYP
                     LVLGWALAVIVYQVGRLIGLG"
     gene            5379..6395
                     /locus_tag="PLM74_05375"
     CDS             5379..6395
                     /locus_tag="PLM74_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42550.1"
                     /translation="MNVSRNPRRVCTMPLALVIALSCVLALSGCLKVSAPYAVSGIVK
                     DALHGQGVGGVTISFSGGFTPVVTASDGTWAKPGLKGIVTMTPSLPGAVFSPSSRTVN
                     NATSTVNVTAAPILESVYWYSVEDEGGFSGGSSPVEPTEELTSVYSVTVENSTAQTAV
                     PQGYDVLVISDTGASFDISDFEGSVIVTFDSGISPLFHYLTGSTQKEVYWDYDSGEEL
                     TWVNPITATELGAEGDARVYHTLSGLSGFTRRNDLLEAGSTDDADDVIVYEVVSGEKA
                     WWWVHIGPHFGDYPDDPTVTDVRVSEIICYILRVLNFGSPDFPEPPVSSDGAGLCGKT
                     SRNK"
     gene            complement(6733..>7968)
                     /gene="feoB"
                     /locus_tag="PLM74_05380"
     CDS             complement(6733..>7968)
                     /gene="feoB"
                     /locus_tag="PLM74_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009611244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HON42551.1"
                     /translation="AESPSQRIDRIVLNKWLAYPIFFGVVWAMFQFTFSLSAPVAGRL
                     GGAIGALGSWVARVLGAAGTPEMVVAFLVDGVIAGLGALLEFAPPIFLLFFAISFLED
                     IGYMARAALISDRLMGAVGLHGKAFIPMILGFGCNVTGILAARSLDSPRDRLVSILVN
                     PLISCSARLPVYVLFASAFFPERRGLVVFSLYALGVVLAALVAKVLSVFVKPDVSSTF
                     VMELPPYRMPRVSGVLIQTWERGREFLHKAGTVILLAVVVVWALSNLPPGVAPGSPDS
                     LIGRIGSAIEPLLRPAGFGSWQAAAALVFGAIAKELIVGTLGVLYGSGVASGVASGSG
                     AGEIGLIGAIQANWTPLSAYAFLVMSLVYMPCVATIAAIRRETGSRGWTAFAMAYPLV
                     LGWALAVIVYQVGRLIGLG"
BASE COUNT         1647 a         2280 c         2511 g         1530 t
ORIGIN      
        1 tggtacggca tcagcgtttt cccgttcagc tgcaatatgt ggatatccgg ggcgcttcgg
       61 cctttgtatc cgggccaatc catggatatg ggtaacgtgt agaaggaaaa cagccggttt
      121 tctgcgcggg ctgttcagaa tgtgcccggt tttgacaggc aggagccgga gcccagcgga
      181 tttgggactt attaggggtc tgaaacccgt ctccggggcc tttccgggga gactcggaag
      241 gcgtgtgcgt atgaccatat tatgtaactc gcctctacct gcgggtttgc cgaggccttg
      301 gcgccagaaa aaggaggcgc cgttgccggc gcgaattctg acaattagcg cccaccaaac
      361 caggcaggat ctgaacagga tgcttgaaaa gcctgcccag atatggacag gatgccccag
      421 agccacgcta gagccctccg cggttcggca cgatcggccc aaatcggggt gcgttttggg
      481 ggatcgcgga gctcccgttg gcggttcccg gcggcccact cctgtctgac gaactgcaga
      541 agtcctgttg acagcataat ggctatattc gaacacagcc atgaaaggtg aaggtggctg
      601 attgaatcgc cgcgcatacg cgtgacttca gtcgtggggg gttcaagtgt tgtttcgccg
      661 ccatgtcgtt tggccgcgcg gggatcgaca aggtcttctt gaccgacggg ctgccgtact
      721 tctggcggta ctcgagcgtg tcaacatcga ccagagcgat agattggccg ggatgcgtct
      781 cgatgcgctc aggcggcgaa ggttccggga tcaggtcctt gtcgtctttc agtgagcaga
      841 ggcaaagccc cagagcatca tgggccatct cgactgctta ctcagaacta tccccttcag
      901 tgcgacagac tggaagatcc cgaaatgtca cagagtagcc gccaacatct ccatagcgaa
      961 aaactgcagc gtacacgtat ttgctcattc aggagatctc catcgctgta ggctgacggt
     1021 tgaccgtatg tgggctagtc gggctagtcg agttctgcct gcttgagggt gctgctaagg
     1081 gttcctggct tcaggtcgcc cggtttcgca gggcgcgcct ccagataccc acatgctaac
     1141 acgtatcatt acgtatgtct tcgtgcgggt cggcctgcgc aagcacgcac gcatgggcga
     1201 gccgacgctt agtgcttccg atggcacaat acgccaccgc agcatcgcag gagaacatca
     1261 catttccgga gaaggactag gccgaatggg actgggctgt tcgggccctt ttccgtgcta
     1321 ggaggacggg ctagggggac gggtatggat cttctgattg acgcggatat cttggcctgc
     1381 cagtttgcat acgcgtatga agccagacgg ggcggcggcg gtgtgcgtgc ggctgtggct
     1441 gttgacgggg atatggacgg cgaagcgcgg gacgatgggc tgcgcactga cggaaggtgg
     1501 gcgattgccg ggttcaactc ggctgttgag cgcatgctga gagagacgcg gactgacaag
     1561 gcgattctct gcttcaccgg ctgcgagaat ttccggtacg ccatactgcc gacgtacaag
     1621 gcaaaccgca ggggcacgcc caggcccgtg ctgctggatg tgctgatcga gcacgcacgg
     1681 caggcgtggg agtgcagatc agaggatagc ctggaggcag atgacgtcat gggcatcatg
     1741 gccaccagat cgccgcggaa ctatgtcatg gccagcaccg ataaggacct gaagcaggtg
     1801 ccgggagtcc actacaactg gcgaacggac tgcatggagc gtgtgacgac gccggacgcg
     1861 gactactggt tttactttca ggtgttgacc ggtgattcga ctgacggtta ctcgggatgc
     1921 ccgggtgtgg gcccgcgccg ggcgaaaacg gtactcgaag gggccggcgc ccacccctgg
     1981 gcttcgattg tcgcggcctt cgagtcgaaa gggctgaccg aggaagatgc gctcgctcag
     2041 gccagggtgg ccaggatact gcgatgcgat gattacgatt ttgtgcggcg caggccgata
     2101 atgtggcgcc cgcgccttga ctggacctga gggaggcgaa cccgcgatgg ttgccaggcg
     2161 taggagatcg acgaaagaga tgacgcagtc gcgacagacg cggcgggtgt ggcagaggat
     2221 aggcctggct gcggtggttc tgatagtcgc tgtggcggcg tctggctatt acctcatcta
     2281 caagagtatg gctgaactgc cggccgaggg cgctatagcc ggacgggtga cagcgaccac
     2341 cgggttcata ccatacagca tgccgggtat tgcgggtgtg actgttgcgg cggagacgct
     2401 tgattcgcgg cttgacgcgg aggatgcgtc agggaccctc gacgccgcgg gtcaggcgga
     2461 ggggcctgtg agttcgcccg gaagatacac tgccaagaca aatggacagg gcgtcttcag
     2521 cctggatgtt ccgcctggcg tgtatgtggt gactttcgaa aaacgcgggt tcgaaacgta
     2581 cagcgaacgc gtgatcgcca tgccgtccgg gcaagctccg gtgttcgtat cactctttcg
     2641 cgaaccccgc ggcaaggctg tggccaggct caagggtccg aaggcttcga agggccccgt
     2701 cccctaccat accagcgtga ggatcgacgc cacaggctca aagaacgtgt ctcgctacgg
     2761 agtgcgctgg gaggttcggg atgcgactgg ctcggttttg atggatccgt atgcgagcac
     2821 tgcagagccg ctccagcttg agccatctcc cattccgggc agctctgcgc tggatttccg
     2881 gttcgtcccg cttgagccgg gggagtacac agtcaccgtc ttcatgcgca accagctttc
     2941 agatgaagaa agctcagcct cggtgacact catcgcggcg aacactgcgc cgacggctat
     3001 agccaacgtg atggctggtc ccgggcgccc atcgaagaca ccgagtggcg ccctgaagcg
     3061 gagcccgggc cttgccaccg ttgtggtggg cgcgcctgtc tttcttgagg ggtggggtgt
     3121 ggatgtcaat gccccttcgc cggaaaccta caaccccggc gggcgcatcg cggacgccta
     3181 cggcaagaac gacgactggg accagcggca gttcgcgtgg gactggaggc tagaatacgt
     3241 gtcgccggac ggtcttcgct caggtgtgac tgacgcgcta ttgtcgacgg aagatgtaga
     3301 agccgatgcg accgatggag ccaatacggc caaagggctc cagcggccct ggttcgttgc
     3361 cgatcgtgtt ggcgagtacg tggcctacgt caccgtatcc gaccgcgatc ccttcggaga
     3421 gccactatcg ggcagcgcgt ctgtgcgcgt acgagtggta gagcccgagg cggtagcggc
     3481 gtcggaggcg gcgtgcgtcg agtgccacaa ggcacaggcc aacccggtcg ggaacatgtc
     3541 ctgccagtgg tgccacggcc ctgcagagcc gcaccttgca gccaagggcg accgagagaa
     3601 gcgcgaaacc atggaggttt cttacgattc tgggctgtgc ggccagtgcc atgctcagta
     3661 tgcagaatgg gagaagtcgc gtcactcaga tggttacccc ttcggctacc atgagatcgc
     3721 caggccgctt ctactcaact gcgcgaaatg ccactacccc gagggatttg ttgaggcggc
     3781 cgaccgggct gacaagcatg gcatgtcatt tggggaggtc cagttcaaac ggccgatgtt
     3841 cgccggcgga cccctgttct tcgactttag ccaactcccg cctgagcagg gcgagggcat
     3901 cgcctgcctg tcctgccacg acccgcaccc cggcgacggc gcaggcgcgt gttcggcggg
     3961 gctgagggtg aacaagtcgg agctatgcgg cacgtgtcat gaggagaaat ggcagaatgt
     4021 cctgctgaac ggcactgccg gccaggtggg aagtgcattc gaataccagg ggacggacta
     4081 ccgggtagcg aatccccacg ataccgaaga gaagtgtgtg ctttgtcaca tgaacgacgc
     4141 cgaggtgggc gcggcggcga ccgactcggc tggtgtggcg gagctaggcg gccacacaat
     4201 gaggatgagg gcgggggcga gcggtggcga tgttttgaac cttgagccgt gccggacttg
     4261 ccactccgac gcggatacat tcgacattaa cggcaggcag tcagagatac gcgctctctg
     4321 tgaggagctg ggatcgctac tgcgtgcccg gaacaatggc gtcctgccag ggtacaggcc
     4381 cggagacaag tgcgccacat gccaccgcgg cggcacgctg ccgttcgacg acgacccccg
     4441 tcttgtgttg gaaaacgcct acaccaacta caagctggtc atgaatgacc gcagctgggg
     4501 agtgcacaac tacgagtaca cgaggcgcct gcttgaagac tcgatcgagt cggtgcgctc
     4561 gattagccca ggccaatgag gcgtccgact tgataaacga tgactgcaag cgcccatccg
     4621 agcacgagcg gatatgccat ggcgaaggcg gtccatcccc gtgagccggt ctcgcgccgg
     4681 atagccgcta tcgtggcgac gcacggcata tacacgagcg acatgaccaa gaacgcgtag
     4741 gccgaaaggg gcgtccaatt ggcctgtatt gcgcctatca gcccgatttc gcctgcccct
     4801 gaccccgatg ctacacctga cgctacacct gacccataca gcactcccaa agtgcccact
     4861 atcagttcct tggcgatggc cccgaacacc aaggccgctg cggcctgcca agagccgaat
     4921 ccggcgggcc tcagctgagg acgtgtttgt gcctgtgatc aacggtgcct gtgtttctcg
     4981 ggccgttgtt ctaggcattg ctcatgacat atggcgccga tgtgcttcac gggccgttgg
     5041 atcttcaggc acattgagat cctccagcga cgccggcgca acacctgcgc tgccgcagtt
     5101 gcagtgcctt atgagctcat gcgaccatcg cgcattgcgg cgactcctcg ttgccagttc
     5161 cgcgtctgtg agactaccgg atgggtatac accgactcac gaaccctctg cttgtatcat
     5221 gcgcggcaga ccatggtcag acgttcgact ctgatgtgcg atactgggcc acggaacttt
     5281 ccgatattaa gaaggaactt tgtgatctac caggaatata ggaacaactt atcagttcga
     5341 agatgtgcct agtaaatgag gattgtgagg tatccaagat gaacgtttcc cgtaaccctc
     5401 gtcgtgtctg tacgatgccg ttggctctgg tgatcgcttt gtcgtgtgtt ctagcgctat
     5461 cgggatgcct gaaagtatct gccccgtacg ctgtgtcggg catagtaaag gatgcgcttc
     5521 atgggcaagg tgttggtggc gtaaccatct ccttcagcgg aggttttacc cctgtcgtta
     5581 cagcatccga tggtacatgg gcaaagcctg ggctcaaggg aatcgtcact atgaccccat
     5641 cactacccgg cgcggtgttc tcaccttcct cgaggactgt gaataacgcc acgtccacag
     5701 tcaacgtcac agcagcgccg atactagagt ctgtttactg gtactcagtg gaggacgaag
     5761 gcggtttctc aggcggatcc agccccgtcg aaccgaccga agaactcacc tcggtctatt
     5821 cggttaccgt ggagaactct acagcgcaaa ccgcagtccc gcagggctac gacgttttgg
     5881 tcatttccga tacgggagct agtttcgata tcagcgattt cgaggggtcg gtaatcgtga
     5941 ccttcgacag cggtataagc ccgctgttcc actacctcac cggcagtacg caaaaagagg
     6001 tgtattggga ctacgactcc ggagaggaac taacctgggt caaccccatc accgccaccg
     6061 agcttggcgc cgagggagat gcgagagtct accatactct gtcgggactg agcggattca
     6121 ctcgaaggaa tgacctgctg gaggcaggat cgactgacga cgcagatgat gtcattgtct
     6181 acgaggtcgt aagtggagag aaagcctggt ggtgggtgca cattggtcca cacttcggcg
     6241 actatccaga tgatccgacc gtgaccgacg tcagagtatc agagataatc tgctacatac
     6301 tgcgcgtact caacttcggc tctcccgatt tccctgaacc gcctgtgagt tccgacggtg
     6361 ccggcctctg cggcaagacg tccaggaaca agtagagcag gataagcaca gaaaccaaca
     6421 tcgcaggccg gggcatgtcc tcggcctctt tcatatgggc ctaccatcga tcagtgcttt
     6481 gttgcctaac gtgaagagtc cctctgcaga catcgcccgg ccagcacctg tgaccagcct
     6541 ttcgacctcg aagatgttgc ctgtctgacc agggagagcg acgtactcag tgaactcata
     6601 aagcgcgcgg cagacagcac ctgtacacaa gatgcgtgtc cgagacagcg gggttctgcc
     6661 agggtagata cccggtgaga ggcggctata cgaggcgcct gcttgaagac tcgatcgagt
     6721 cggtgcgctc gattagccca ggccaatgag gcgtccgact tgataaacga tgactgcaag
     6781 cgcccatccg agcacgagcg gatatgccat ggcgaaggcg gtccatcccc gtgagccggt
     6841 ctcgcgccgg atagccgcta tcgtggcgac gcacggcata tacacgagcg acatgaccaa
     6901 gaacgcgtag gccgaaaggg gcgtccaatt ggcctgtatt gcgcctatca gcccgatttc
     6961 gcctgcccct gaccccgatg ctacacctga cgctacacct gacccataca gcactcccaa
     7021 agtgcccact atcagttcct tggcgatggc cccgaacacc aaggccgctg cggcctgcca
     7081 agagccgaat ccggcgggcc tcagcagggg ctctattgcg ctgcctattc gccctatcag
     7141 cgagtccggg ctgcccgggg cgacgcccgg gggcaggttg gacaatgccc acactacgac
     7201 caccgccagc agaatgactg tgccggcttt gtgaaggaac tcccgacccc tttcccatgt
     7261 ctggatcagg acccctgata ccctgggcat tcggtatgga ggaagctcca tcacgaatgt
     7321 agacgagacg tcgggtttga cgaagacact cagcactttg gctactaacg ctgccagcac
     7381 aacacctagc gcatagaggg agaacactac aagcccgcgg cgctcaggga agaaggcgct
     7441 ggcgaacagg acgtacaccg gcaatcgcgc agaacatgag atcagcgggt tgacgagtat
     7501 cgataccagc ctgtcgcggg ggctgtccag gctgcgcgct gccagtatac cggtgacgtt
     7561 gcatccgaaa cccaggatca tggggatgaa cgctttgccg tgtagtccaa cggcgcccat
     7621 gagtctgtcg gagataaggg cggcccgtgc catgtatcca atgtcttcaa gaaatgatat
     7681 ggcgaagaag agcaggaata tggggggcgc gaactcgagc agggcgccaa ggccggcgat
     7741 gaccccgtct acaaggaaag cgaccaccat ctccggcgtg cccgcagccc cgagcacgcg
     7801 cgcgacccag cttcccaagg ctccgattgc gcccccgagc cggcccgcta caggcgcact
     7861 cagcgagaac gtgaactgga acatggccca caccacgccg aagaagatgg ggtatgccag
     7921 ccacttgttg agcacgatgc ggtcgattcg ctgagacggc gactccgc
//
LOCUS       DAOTAC010000094         7907 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_7821_length_7907_cov_4.447529, whole genome shotgun sequence.
ACCESSION   DAOTAC010000094 DAOTAC010000000
VERSION     DAOTAC010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7907
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7821_length_7907_cov_4.447529"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="PLM74_05385"
     CDS             <1..247
                     /locus_tag="PLM74_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42552.1"
                     /translation="QLIESAMRMRDMYNMGDLDTGNLDTDDHGTGDHDTGSDVTRDRV
                     TRDRVTRDRVTGDCDTGDYSTDDVIHLSGGDMDADGR"
     gene            231..1280
                     /locus_tag="PLM74_05390"
     CDS             231..1280
                     /locus_tag="PLM74_05390"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HON42553.1"
                     /translation="MLTEDSCLTDEMFRRVIAIVNLLMSAPEGLSVADLASAVGAAPS
                     IVRGDLLRLAEYGRAPLAAGCADDEEPDKEYENDELACDPQNEVWSLASRDFAFPVTG
                     LSQLEAQTLLEILSRAQGADHVKQRLQASLASSGVRRRVVKAGRTIYGDGQEESKIDE
                     LGAYVAERAPIRLVYIDRHGRPTQRDTCPTALVYDWRTCAWYLYGHNGSSSDGGGFRH
                     FRVSRIQSFGPAMSYVAAPDDSLVEEHVKSCWGVECSSTPVKVVVRFADDFNVLDRLY
                     ADTADRPEAQYETEPDGSVIYRDIMPGCNEFRAWVATFGESAEILEPAALRHSMALAV
                     QRVLDRYAELDCMPK"
     gene            1461..2639
                     /locus_tag="PLM74_05395"
     CDS             1461..2639
                     /locus_tag="PLM74_05395"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HON42554.1"
                     /translation="MKLLKILHTSDIHLDHVLTDYNRDCRNARRAELVSAFDFLVDEA
                     IRQTADIMLIAGDLLCARSVSDSTASAVREGFRRLARASIFAAVVPGEAEEVHSLAML
                     REVCSEPNVHLFSGEEWSRVSPLPGVSVWGLRTTSRNSTTNALANLVVEGSGVHIGLM
                     HGTFSGTPGLDSSISPVTPTELSQSGLDYLALGHYHNMVNNSVGRATCWYSGSPVHLG
                     FDTRGERHALIVTVRGDGVRVYPMKVPGRPQRVVNIEVTATRAREQLYARLARLASSE
                     LCLRAEVEGETEPGDVGLVDRAEQDFRSRFFHLTVADHTTVSCPPWRSSDLRSTHLDE
                     AFFRKAVRLTAEPGAARKPPEVIDRALKLGLGALGEVDTFAHRETRVQGQGQAAGDPD
                     "
     gene            2581..4983
                     /locus_tag="PLM74_05400"
     CDS             2581..4983
                     /locus_tag="PLM74_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42555.1"
                     /translation="MLIERLAFKVRARRRAIQTDLAPGLSVVSVDDPAVARAFVSALR
                     WTDDFAGVLWADLALVDGSGSRIALALERGADGRILRRATEQSGGALDPIDVPSRHVM
                     AFALPTEGGAARPYPDGRTALLSLPLSEVLPVAETRLCAAIAAMDSMVTRTCTQEDAG
                     SQACDAGRQTHRTRLEALVDQAHSLRARRVALESRLSWLAERVSFIDSRLSYIESFLI
                     ACAERDDIQAYIERLKTYFRECSQRLLSAETARAALEEASARRASCPGNDGAFEPAIA
                     SEVRSLDGSLRELERKAQDCEARLSRASAWVARLKVEQALVHEKIRSAHGPDRSEHMA
                     SQARTLTAIILDKEEALDRVASQIASADRVLMQRRLAERMTIAGAMVAALSLMAIILP
                     ANVWPSRAASIPETGVSILLALMAAGLLSTVYGIRRGIEIEGRAEARAALDKERAALA
                     RQLAWNRQQLSSMLGGHTLDEYVDDLNERRRLEMRRDAIADEMKEALADSARLHNLAD
                     FVRVSAVRARERLAEILRATGFTSASSYLDAYERYRRLDWAFQDALGRLESALGGLTE
                     AGIEMDMEIVAEEIAQAEAGRDALFDARANGRLGELSEEYALKAAERERLTSELAACS
                     LELEHTHSELGAIDLWEAASDAAEAAIDDERAAAQKEAAACARELLQELLDARAPEAC
                     MRLAESVSEILRFITEARDASVTCAVEDGKAVFFPEGARRADECMRQQARLAVELAIR
                     ESADGRDVVPLVVEHLSPTPILAGLLAALRRVSLTRQVILLASPGALPECAPTDHVVT
                     IA"
     gene            5073..6311
                     /gene="thrC"
                     /locus_tag="PLM74_05405"
     CDS             5073..6311
                     /gene="thrC"
                     /locus_tag="PLM74_05405"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HON42556.1"
                     /translation="MKSFACVSCQAQYGSLPSTYTCPACGGILDVVYDYGSIDGGRFV
                     STLAASSDRSIWRYEDLLPVTPGGPRPKLRVGGTPIYHAARLAEEIGLSDVFIKDDGV
                     NPTASLKDRASAVAVAMALEAGAAVISCSSTGNAASSCAGCAASVGLPAVIFVPERAP
                     AGKLAQLRIFNAVVIRVQGSYRDAFNLSREAIKRYGWYNRNAAINPYMVEGKKTVSFE
                     ICEELGSALPDWVAVSVGDGCTVAGVWKGFRELHDIGVIPRTPRILGVQAEGSNPISV
                     SFRTRRPMQPGREDTIADSIAVGEPRNPVKAVNAVRDSGGTMIDVSDEEILSAMRLLG
                     RTTGVFGEPAGVAGLAGLARAVREGIVDSSASACAIVTGNGLKDIQTGIRAAGEPITI
                     PPDIALLERELAQVAPSVLP"
     gene            6377..7777
                     /gene="hydA"
                     /locus_tag="PLM74_05410"
     CDS             6377..7777
                     /gene="hydA"
                     /locus_tag="PLM74_05410"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HON42557.1"
                     /translation="MYDLAILGGNVVSSDSVEVADVLIKDGVIVQVLPAKECGGASAR
                     GAVNRARAEREVDARGMLVMPGVIDPHVHFELRSRGTVTADDFASGTRCAAAGGVTTV
                     IDYADHLPGTTLSQCAAARLSEIRPQAAVDYAVHQNCIRIDHDIGDQLAELCRAGIGS
                     VKVFTTYKDAGYMLEGGDLEALLRAAKHHDVLVTVHAEDDALIEQARGRLEAAGHVSP
                     EYHGESRPAEAEERAVRNVIAAAKAADAPVYFVHVSTRGAMEAIAEGRREGQQVMAET
                     CPHYLLLDEGRYSGADARLFIMSPPLRTQADHKVLWAAAAAGGIDTFSTDHCAFTPEQ
                     KALGRSCFDTLPGIPGVQTLLPLIYTYGVATGTITVGQMVKMMCENPARLFGISCRKG
                     RIAPGLDADIVILDRAARRRLRGVELLSQAGYTPYEGMEVSCDVREVYLRGNLVAAHG
                     RFCGACGAGEFVRASA"
BASE COUNT         1523 a         2241 c         2598 g         1545 t
ORIGIN      
        1 gcagctgatt gagagcgcga tgcgcatgcg cgacatgtac aacatgggcg acctcgatac
       61 aggcaacctc gatacggacg atcacggcac gggcgatcac gatacgggca gtgacgtgac
      121 acgcgaccgc gtcacacgcg accgcgtcac acgcgaccgc gtcacaggcg actgcgacac
      181 aggcgactac agcacagacg acgtgataca cctaagtggg ggcgacatgg atgctgacgg
      241 aagatagctg tctgaccgac gagatgttca ggcgcgtgat cgccatagtc aaccttctaa
      301 tgtcggcgcc ggaggggctg agtgtggccg atctcgccag cgcggtgggt gcggcgccat
      361 ccatcgttcg cggcgacctg ctccgcctgg cggaatacgg tcgtgctccg cttgcggcag
      421 gctgcgcaga tgatgaagag ccggataagg agtacgagaa cgatgagctg gcttgcgacc
      481 ctcagaatga ggtatggagc ttggcatcgc gggacttcgc cttcccggta accgggttat
      541 cgcagctcga ggcccagacg cttctggaaa tcctctctcg agcccagggc gccgatcacg
      601 tcaagcagcg acttcaggcg agcctggcaa gtagtggagt gcgcaggcgt gtggtgaagg
      661 ccggccgaac aatatacggt gatggccagg aggagtccaa aatcgacgag cttggggcct
      721 acgtggcaga gcgagctccg attcggttag tctacatcga ccggcatggc cgccctaccc
      781 agcgcgatac atgccccact gcgctggtgt acgactggcg tacctgtgca tggtatttgt
      841 acggccacaa tggctcaagt agtgatggcg ggggattccg tcatttcagg gtgtcgcgca
      901 tccagagctt cgggccagct atgtcctacg tggcggcgcc cgacgacagc ctggttgagg
      961 agcacgtgaa atcatgctgg ggcgtggagt gcagctctac cccggtgaag gtagtcgtgc
     1021 gtttcgccga cgactttaac gtgctggatc ggttgtacgc cgatactgcc gacaggcctg
     1081 aagctcagta cgaaacggaa cctgatggat ccgtgatcta ccgcgacatc atgcccgggt
     1141 gcaatgagtt cagggcatgg gttgctactt tcggcgagtc ggctgagatt ctggagccag
     1201 ccgccctccg ccacagtatg gcactggctg tccagagagt gctcgacagg tacgccgagc
     1261 ttgattgcat gcctaaatag aagggaaacg gcgcagcggg gcgaactata tagcagtcgc
     1321 gggctgattg agcctagcga cgtatagcga gccggtatcg gctgtaaagg cgaaattccg
     1381 cggaatacag ttggcaactg tgtggggttt cgcctattgt cgcagtgtat cgatacgtat
     1441 ctctgctggg aggctgcgcc gtgaaattac tgaaaattct gcatacatcc gatattcacc
     1501 ttgatcatgt ccttacagac tacaacagag actgccgcaa tgcccgccgc gccgaactgg
     1561 tgtcggcatt tgacttcctg gtggatgagg ctattcgtca gaccgccgac atcatgttga
     1621 tagcggggga cctgctctgc gcgcgttcag tttcggattc cactgcatcg gccgtgcgcg
     1681 agggttttcg taggctcgcg cgtgcctcga tctttgccgc cgtagtgccg ggagaagctg
     1741 aggaagtaca ctcccttgcc atgcttcgcg aggtgtgtag cgaacccaat gtgcacctat
     1801 tttcgggtga ggagtggtcg cgcgtatccc ctctgcccgg agtcagtgta tggggtctgc
     1861 gcaccacctc gcggaattct actaccaacg ccttagccaa cctggttgta gagggctcag
     1921 gcgtacacat cgggctcatg catggcactt tctctggcac gccgggcctg gattcctcca
     1981 tatcgccggt cacgccgact gagctatctc agtcgggtct ggactacctg gctctgggcc
     2041 actaccacaa catggtgaat aactcggtgg gccgtgctac ctgctggtat tcaggctctc
     2101 cggtccatct aggcttcgat actcgcggcg agcgccacgc attgatcgtg accgtgcgtg
     2161 gcgacggggt ccgtgtatac cccatgaaag tgcccggtcg tcctcaacgg gtggtcaata
     2221 tcgaggtcac cgccactcgt gcgagggagc agctatacgc tcgcctcgcg cgtcttgcca
     2281 gctccgagct atgcctcaga gccgaagtcg agggcgaaac ggagccaggc gatgtggggc
     2341 tggtggaccg ggccgagcag gacttccgga gtagattctt tcatctgaca gtggcagacc
     2401 atacgacggt tagctgcccg ccgtggcgtt cctctgatct gcgtagcacg catctggacg
     2461 aagcgttctt ccgcaaagct gtccgactga cagcagaacc cggcgcggcg cgaaagccgc
     2521 cagaagtcat cgatcgtgca ctcaagcttg gactgggcgc tctaggggag gtggacacct
     2581 ttgctcatcg agagactcgc gttcaaggtc agggccaggc ggcgggcgat ccagactgac
     2641 ttggcgccgg ggctgtctgt tgtgtcggtg gacgatccgg cggtggcccg agcgtttgtg
     2701 tccgccttgc gctggacgga cgacttcgca ggtgtcctct gggccgacct ggcactggtt
     2761 gacggcagcg gctcccggat cgcgctggca cttgagaggg gggctgacgg tcggatcttg
     2821 cggcgagcta cggagcaaag cggcggcgcc ctggacccga ttgacgtacc gagccggcat
     2881 gtcatggcgt ttgccctgcc gaccgagggc ggcgcagcca ggccgtaccc tgacgggcgc
     2941 acagcccttc tcagcctgcc gctgtcggag gtgctgcccg tggccgaaac caggctgtgc
     3001 gcggccatag ccgccatgga cagcatggtt actcggacct gcactcagga ggacgcaggg
     3061 agtcaggctt gcgacgcagg caggcagacg caccgcacgc gactggaagc cctggttgat
     3121 caagcacaca gcttgcgcgc tcgacgggtc gcgcttgagt ctaggctgtc gtggctcgcc
     3181 gagcgcgtga gctttatcga cagccggctg tcatacatcg agtctttcct gattgcatgt
     3241 gccgagcgtg atgacataca agcctacatc gagcggctca agacgtactt cagagagtgc
     3301 agccagaggc tgttatctgc agaaaccgcc agagcggcgc tggaagaggc cagcgcccgt
     3361 cgtgctagct gcccgggcaa tgacggcgcg ttcgagccgg ctatagcatc cgaagtgcga
     3421 tcgctcgatg gatcgctgcg agaattggaa agaaaggctc aggattgcga agccagatta
     3481 tcgcgcgcat cagcctgggt ggcccggctc aaggtcgagc aggcgctggt gcacgagaag
     3541 atccgctcgg cgcacggccc tgaccgttcg gagcatatgg cgtcgcaggc tcgaacgttg
     3601 actgctatca ttttggacaa ggaagaggcg ctcgaccggg tcgcttcgca gattgcgtct
     3661 gccgaccgag tgctcatgca gaggagactg gcagagagga tgacgatcgc aggcgccatg
     3721 gtagcggctc tgtcgctcat ggctatcata ctgcctgcca acgtgtggcc gtcgcgcgct
     3781 gcgtcgattc ccgaaaccgg agtatcaatt cttctggcgt tgatggcggc gggcctgctg
     3841 tcgacggtgt acgggattcg ccggggaatc gagatcgagg ggcgcgccga ggcacgggcg
     3901 gccctggaca aggagagggc tgcactggcc aggcaactcg cctggaacag gcagcaactc
     3961 tcctcgatgc tcggcggaca cacattggac gaatacgtgg acgatctgaa cgagcggcgt
     4021 cgcctggaga tgcgaaggga cgcaatagct gacgagatga aggaagcctt ggccgattct
     4081 gcccgattgc acaacctcgc cgactttgtg cgcgtgagtg cagtgcgggc gcgcgaacgg
     4141 ttggccgaga tcctgcgcgc gaccggcttc acttcagcct cgagctatct ggatgcatat
     4201 gagcggtatc gccggctcga ctgggcgttt caggatgccc tgggcaggct cgaatccgcc
     4261 ttaggggggc tgacagaggc cggcatcgag atggacatgg agatagtcgc tgaggagatc
     4321 gcccaggcag aggccggtcg agacgcgctt ttcgatgcca gggcaaacgg ccgcctcggg
     4381 gagctttcgg aggaatacgc gctcaaggcc gcggaaaggg agcgcctcac cagtgagctt
     4441 gctgcatgtt cgctggaact tgagcacaca catagtgaac ttggcgccat cgatctgtgg
     4501 gaggcggcta gtgacgcagc cgaggcggcg atcgacgacg agagagcggc tgctcagaag
     4561 gaggccgcgg catgcgcccg cgagcttctt caagagctgt tagacgcacg tgcccctgag
     4621 gcctgcatga ggttggctga gtcggtgtcc gagatcctcc gcttcattac ggaagctcgc
     4681 gacgcaagcg taacctgtgc cgtggaagat gggaaagcag tgttcttccc ggagggcgcc
     4741 cgcagggcgg acgagtgcat gcgtcagcag gcccgactag cagtggaact ggctatcagg
     4801 gagtcggcag acggacgtga cgtcgttcct ctggttgtgg agcacctgtc gcctactcct
     4861 atcctcgccg ggcttctggc tgcccttcgc cgagtgtcgc tcacacgtca ggtgattcta
     4921 ctcgcctcgc cgggcgcact gcctgagtgt gctcctaccg accatgttgt gaccattgca
     4981 tgatcaaatg gagggaatcg agatgatgcg ttgaattgcc atactatgca gtcatacaga
     5041 ctgccaggga ggcttgacgg ttgatgaatc ggatgaagtc cttcgcttgt gtctcatgcc
     5101 aggcccagta tggctctctt ccttccacat atacgtgtcc tgcgtgcggc ggcatactcg
     5161 atgtcgttta tgattacggc tcaatcgacg gcggtcgttt cgtgagcact ctcgccgcga
     5221 gctccgacag gtcaatatgg cggtatgaag atctcctgcc tgtgacgccg ggcggccctc
     5281 gcccgaagct cagagtaggc ggaacgccca tctaccacgc ggccaggctc gcagaggaga
     5341 tcgggctttc ggatgtgttc ataaaagacg acggcgtcaa ccccaccgct tcactgaaag
     5401 accgcgcatc ggctgtggcc gtggccatgg cgctcgaagc cggagccgcc gttatcagtt
     5461 gttcttccac gggcaacgct gcatcgtcct gtgcgggctg tgcagcgtcg gttggtcttc
     5521 ccgccgtgat attcgttccc gagcgcgctc cagccggaaa actggcgcaa cttcgcatat
     5581 tcaacgccgt tgtcatacgc gtgcagggta gttacagaga cgcgttcaac ctctcgcgcg
     5641 aggcgattaa gcgctatgga tggtacaacc gcaatgccgc tatcaacccg tacatggtcg
     5701 aaggcaagaa gactgtttcg tttgagatat gcgaggaact cggttcggcc cttccggact
     5761 gggtggcggt atccgtggga gatgggtgta cggttgcagg agtatggaag gggttccgag
     5821 aattacatga tattggcgta atacctcgga cgcccaggat cctcggcgtc caggctgagg
     5881 ggtccaaccc catcagtgta tcgtttagga cccgaagacc catgcaaccc ggacgggagg
     5941 acacgatcgc cgattcgatt gcagttggcg agccccgaaa tccggtgaag gcggtaaacg
     6001 ccgtgcgcga ctcgggaggc acgatgatcg acgtgtccga tgaggagatc ctctctgcga
     6061 tgaggctgct aggtcgcaca acaggggtct ttggggaacc ggcgggcgtc gcgggtttgg
     6121 cggggctggc gcgtgccgtg cgcgaaggca ttgtcgattc atctgccagt gcctgtgcga
     6181 tagtgacagg caatggccta aaggacatac agacaggcat ccgcgcagcc ggtgagccta
     6241 tcaccatccc accggacata gctcttctcg agcgagagct cgcccaagtc gcgcccagcg
     6301 tcctgccgta atgcgctgcg tgacctgcag cgcgggccgg gtgagcctga cacggatcta
     6361 gatagaggtg tagtgtatgt atgacttggc aatactgggc ggcaatgttg tatcatccga
     6421 ttcagttgag gtcgccgatg tcctgatcaa agatggtgta atcgtccaag tcctgcctgc
     6481 caaggaatgc ggcggtgctt ccgcgcgcgg ggcagtgaac cgcgcccggg ctgagcgaga
     6541 agtagatgcc cgcggcatgc tggtgatgcc gggcgtgatc gatccccatg tccacttcga
     6601 gctgcgctcc cgtggcacgg tcacggcaga cgactttgcg tcgggcactc ggtgtgcggc
     6661 cgcgggcggc gtgaccacgg ttatcgacta tgcggatcac cttcccggaa cgacgctgtc
     6721 ccaatgcgct gccgcacggc tttcggagat caggccgcag gctgccgtgg actatgccgt
     6781 ccaccagaac tgtatccgaa tcgaccatga catcggtgat cagcttgccg agctctgcag
     6841 ggccggaata ggcagcgtga aggtctttac cacctacaag gatgcaggct acatgctgga
     6901 gggtggcgac cttgaggctc tgcttcgcgc tgcgaaacac cacgatgtgc tcgtcactgt
     6961 gcacgctgag gacgacgccc tgatcgaaca ggcccgtggt cggcttgagg ctgctgggca
     7021 tgtctcccct gaataccacg gagaatcgag gccggccgag gctgaggaga gggctgtccg
     7081 gaatgtcatc gcggcggcca aggcagcgga cgcaccagtc tatttcgtgc acgtatccac
     7141 ccgcggggcc atggaagcca tcgcagaggg caggcgtgaa ggtcaacagg tgatggccga
     7201 gacgtgtcca cactatctcc tgctcgatga aggccgatac tcgggcgccg acgcccgtct
     7261 gttcataatg agccctccat tgcgcactca ggcagatcac aaagtactct gggcggctgc
     7321 tgctgccggc ggcattgaca cattctccac ggaccattgc gcattcacgc ctgagcagaa
     7381 ggctctcggg cgttcttgct tcgacacctt gcccgggatc cccggcgtgc agacgctctt
     7441 acctcttata tacacttacg gcgtggcaac cggtacgata accgttggcc agatggtgaa
     7501 gatgatgtgc gaaaatcctg cgcgtctgtt tggcatatct tgcaggaagg gccggatagc
     7561 tccggggctt gatgccgata tcgtcatact cgacagagcg gcgaggcgca ggctacgagg
     7621 ggtggaactg ctctcgcagg ccggctatac gccgtacgag gggatggagg tcagctgcga
     7681 cgtgagggag gtatacctcc gggggaacct tgtcgcagca catgggaggt tctgcggcgc
     7741 atgtggcgcg ggcgagtttg tccgcgcttc agcttgagtg ggtttaggca ggtgggagga
     7801 tggtaactcc cgcgcgtcga gaatatcccc acgcgtgtgg ggctgaatcc gagacttgac
     7861 cgtgaaagga gaacggcgtt tcatggaggc cgtcagggta aggatca
//
LOCUS       DAOTAC010000095         7905 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_7823_length_7905_cov_4.877197, whole genome shotgun sequence.
ACCESSION   DAOTAC010000095 DAOTAC010000000
VERSION     DAOTAC010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7905
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7823_length_7905_cov_4.877197"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="PLM74_05415"
     CDS             <1..227
                     /locus_tag="PLM74_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015553704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HON42558.1"
                     /translation="VPVIKICGNPHTYRRLEGDMDINAGDVIENKRSIEEVGDETFQA
                     LRRVLSGQLTKAEAVNYTVSMDIHTLGPVI"
     gene            288..1478
                     /locus_tag="PLM74_05420"
     CDS             288..1478
                     /locus_tag="PLM74_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HON42559.1"
                     /translation="MLSQSLRFGTQLVHGVDVKEQISSEPLSPPIFQTSAFSFSSLED
                     AAAVMGGTATGCAYSRSGNPTIRRLAATVARLEGAEAGLAFGSGMAAISAALMTLLRS
                     GDEVVAGSVLYGTTYALLTEMLPRFGIITRFVDLTDPELTELQAAISDRTKVVYLESP
                     QNPDLDIIDIKAVARAISGSSAALVVDNTFATPCLQRPLELGADLVVHSATKYLGGHG
                     DLLAGIVVGSADMVTRIAKESATRLGGVISPFVAWLCLRGIQTLGVRMERHCSNALKV
                     AEFLTECSKVQRVRYPGLTSHPQYELAMRQMGAGGGMITMDLADLGEAQRFLKELKLF
                     HLSVSLGDTTSLAQHPASMTHGKIDPAERRIRGIGDGMIRLSIGLEDADDLIEDIDVA
                     LEHV"
     gene            1799..2029
                     /gene="csrA"
                     /locus_tag="PLM74_05425"
     CDS             1799..2029
                     /gene="csrA"
                     /locus_tag="PLM74_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006690315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HON42560.1"
                     /translation="MLVLTRKAKQSIMIGDGIEVTVVEIRGDAVRLAIDAPRQVPVHR
                     KEVYEEIQRENREASAVSVEDAAAIERLLSGC"
     gene            2786..3088
                     /locus_tag="PLM74_05430"
     CDS             2786..3088
                     /locus_tag="PLM74_05430"
                     /inference="COORDINATES: protein motif:HMM:NF015602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HON42561.1"
                     /translation="MSPEGKVNSISGVDLNVSRTAWEWHIEKSSADIRNQVRRANRAL
                     EAVDIQAKFSIHEGTGEIIVKLEDTNTGEVIREIPPEKLLDLVAKMQEMARGMGSE"
     gene            3147..5246
                     /gene="fliD"
                     /locus_tag="PLM74_05435"
     CDS             3147..5246
                     /gene="fliD"
                     /locus_tag="PLM74_05435"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HON42562.1"
                     /translation="MADRLRVSGISTGYDTAQLIEQLMAVERRPVLLMQKQQSQIKTR
                     SDAWRDVASRLANLKSKAAALKSASVFNVKSVASSADKTVSAIADSSAATGTYKVWVD
                     RLATSTKATGSAELGRGLDMGAKLAEAGFSITPTAGTFTINGAQIRIDADTVMSDGVD
                     GASSNSILGKINSSGAGVTASVVANRMVLASNDGVSPVIVGSGGDTSNFLTAARLLGA
                     ASGTTVTSQGSLGVAKTTVALTGARLGAPIQSSLLGGVAEGEIAGKLATALEGTETVT
                     FTYHGTEYTTESLAAEDDMAAVAADLEAKMNAAAGLAAGTIRVRVSGLEEGSKDRFVI
                     TDTQTPTDSAEHSITIGANEGDSPTALRLAALDGAANKGMFKINGVAIEYDRTRDAVN
                     DIINRINLSKANVSASYDLLSDKIVLTAKYTGELAISVEDVGGNFLEAASLLDAEQEY
                     GANALFRVEGLSGGAQLSSASNTVKGIISNVTLTLKSVSDDPATITVSQNVDSASSAI
                     KAFVDQYNSVMDLLATQTAYDKDKKIAGPLQGDGAVRTIQQTLRRLTTTAVDGLPQGL
                     NSLLAIGISTGAAGQGDFRSAQLKVDDARLKELLAQDPEAVARVFADAENGIALKFES
                     YLGELTRGVTGVLAKTQEILSNQTKSIDRRIADVERRLEKRREGLVQQFTQMEKVLAS
                     MQQQQAWMSAQFNSMQV"
     gene            5281..5679
                     /gene="fliS"
                     /locus_tag="PLM74_05440"
     CDS             5281..5679
                     /gene="fliS"
                     /locus_tag="PLM74_05440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HON42563.1"
                     /translation="MMATPCNRYMEMQVRTAPAENLVLMLYDGALRFARQAQVDLAEG
                     RMQDAHNNLTRAQDILGELMGSLNMDAGDIAHNLFKLYEFIQYRLTVANVKKSTEPID
                     DVIQLLGDLRATWAEAIRDARGRSVKSGGQ"
     gene            5681..6205
                     /locus_tag="PLM74_05445"
     CDS             5681..6205
                     /locus_tag="PLM74_05445"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HON42564.1"
                     /translation="MGRNGHEAQLRELAEHIHSCRADELEYYRSILDLSRAQRVAIVE
                     GDTDTLDQATDEKSRLISLIDTLDLKIAGLLDEVSYVTGVHSHACVYPDDGPAAATAS
                     TPIGGCALNARIAGVMAEILEAERANQELLEQAISGVQEELKQIDAGSKAVRAYWGRG
                     GDALHPAPAVDDNA"
     gene            6971..>7905
                     /locus_tag="PLM74_05450"
     CDS             6971..>7905
                     /locus_tag="PLM74_05450"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HON42565.1"
                     /translation="MKKDGFEITDSEAIALFIGDVSGGAPCGGHVLIVDDEVNMQVVL
                     AEAFEDEGHRVSTASSGAEAISAIGAYDFDLLILDLKLPDMTGIDVFRVAREIRPGIV
                     AIMITAYSSIDTAIEAMKMGAYDYITKPFNVEKLLMVAGNAIEGRILSAPRLSHFAEL
                     AVEDEEFGGVVGSSLKMRQLLDICRSIAPTNATVLIYGESGTGKELVASYIHKKSLRS
                     SKPFIRVNCAAIPETLLESELFGHEKGAFTHAISRRLGRFELAHTGTIFLDEVGEMSP
                     AMQAKLLRVLQEKEFERVGGVETIKVDARVVAATN"
BASE COUNT         1775 a         2082 c         2461 g         1587 t
ORIGIN      
        1 ccgttcccgt gatcaagatc tgcgggaatc cgcatacgta tcgccgtctg gaaggcgata
       61 tggacataaa cgcaggcgat gtcatagaga acaagcgctc aatcgaagag gtgggagacg
      121 agaccttcca ggcgctacgt cgcgttcttt cggggcagct tactaaggca gaggcggtaa
      181 actacacggt ctccatggat atccacacgt tgggtcccgt catatagatc atggcccttt
      241 cagtttgact ggaagcacgg catatgatgt tacgaaagga atggtacatg ttgagccagt
      301 cactcagatt cggcacccag ctagttcatg gtgtagacgt caaggagcag ataagtagcg
      361 aacctctgtc gccgccgatt ttccagacca gcgctttttc cttcagttcg ttggaggatg
      421 cggctgcggt catgggtggc acggcgacgg gatgtgccta ttccagaagc ggcaatccta
      481 ctatcagaag gctggccgcc actgttgccc gcctggaagg ggccgaggcc gggctggctt
      541 ttggatcagg gatggcggcc atatcggctg cgttgatgac tctactgcgg tctggcgacg
      601 aggttgtggc aggcagtgtt ctgtacggaa cgacgtacgc gctactcaca gaaatgctgc
      661 cgcgattcgg cattatcacc aggtttgtgg atctcaccga tcctgagctg accgaacttc
      721 aggcggcaat ctcagacaga accaaggtag tgtatctaga atcacctcag aatcccgacc
      781 tagacatcat cgatattaag gccgtcgcaa gggccatatc aggtagcagc gcggcccttg
      841 tggtcgacaa tacgtttgcc actccatgtc ttcagaggcc cttggagctc ggagctgatc
      901 tggttgtgca cagcgcaacg aagtacttgg gagggcatgg cgacttactt gccggcatcg
      961 tggtgggatc ggcagacatg gtgaccagaa tagccaaaga aagcgccacg cgcctcggcg
     1021 gagtcatatc cccctttgtg gcctggcttt gccttcgagg catacagacg ctcggtgtgc
     1081 gcatggaaag gcactgtagc aatgccctca aggtagctga gtttctgacc gagtgctcga
     1141 aggtgcagcg agtcagatat cccgggctca caagccaccc ccagtacgaa ctggcaatgc
     1201 gccagatggg agccggcggc gggatgatca ctatggacct ggccgacctt ggcgaggcgc
     1261 agcgcttcct gaaggaactg aagctgttcc acctatcggt gtctcttggc gatacaacat
     1321 cattggctca gcacccggcc agcatgacgc atgggaagat cgatcctgcg gagcgtagga
     1381 tacgaggaat aggcgatggc atgataaggc tgtcaatagg tttggaggac gcagatgacc
     1441 tcatcgaaga catagacgtg gctctggagc atgtgtagtc tgtagaggct tgagcctgtg
     1501 atcatgagcc cacaccatgt cgattcgtgg ttctgtgcgg gtacgaaacg gcgcagtgca
     1561 gcgagagtag tcgggacagg catgaacgca gtgtgcacga tgggggttcg ggcatgctgc
     1621 gttcttgcat ggatggagta gccgtgtgag ttgagggcgc atgtggccgc cacagaatca
     1681 gtcatcacct gtttagtacc ctggccacgc gattcccctg agaaggtatt gagcactgca
     1741 tgcagaatct agcattgttc gatacctcga ctgtcaatct atccacggag ggatttgcat
     1801 gctcgtgctc acacggaaag ccaaacagag catcatgatt ggcgatggaa tagaggtaac
     1861 tgtggtggag atccgcgggg acgccgtgag gctcgccatc gatgctccta ggcaggtgcc
     1921 tgttcatcgt aaagaagtat atgaggaaat ccagcgcgaa aacagggaag cttcggccgt
     1981 gtcggtggag gacgctgctg cgattgagcg gctgctgagc ggctgctgag cggctgctga
     2041 gcggcaggac agacacgtcg gtggactacg gtgccagtgg tagaatctag gtgtctcctt
     2101 tcggaactcg tttttggcga tgtgtctcga aaaccaacaa tggatagtgc ataatcgaac
     2161 gtatagacgt ataggacgcc tgaggcaaag caggcgtaac atatacgccc atttgggcta
     2221 gctgaagatg ctacatgtta gcgcaatgcc ccatattcca accatataat ctcgccatgt
     2281 gtcattctct ccaccagatt tgtccatagt ccgtctctat gcagatatac gttccgttat
     2341 gaaccaatca ttgtccggat tcgcgacaca tccgccaaga cctgttcgga atacgaacac
     2401 atcccaaaag gccaggcgga tcggcggttc atgacggata gccagcgtga gacgcatgta
     2461 catgccgact cggggatcgt cggtcacagg caatctgcgg tcggcagtca agggtccgcc
     2521 tacggtcgcc gattgcgtgg ggtagagcac caagggaatc cccggttcga gatcctcact
     2581 cgaccactcc aacgttgcct tcccgcatcc ccgcattcga tgcccggtgc gattgatgtg
     2641 aattcggcct aaactccacc ccgcgtctgc cgataagtat gacgaggaac aaatgcgcca
     2701 gcctggacaa cggatttggc caggctcgct tacatatgag gctcgattgt gtattcggaa
     2761 ttctcacaac atggaggtgt gactcgtgtc tcctgagggc aaggtcaact cgatatcagg
     2821 ggtagatctg aacgtgtcga gaaccgcatg ggagtggcac atagagaaaa gctcagctga
     2881 catccgaaat caggtccggc gggctaatcg ggctctcgaa gctgtggaca tccaggccaa
     2941 gttctccatt cacgagggca ccggcgagat catcgtcaag ctggaggata ccaataccgg
     3001 cgaggtgatc cgggaaatac ctccggagaa actcctagat ctcgtggcta agatgcaaga
     3061 gatggcacgg gggatgggca gcgagtaacg gtcattgcct gtggacggcg gattgcagat
     3121 ggccaacact acgggggtga gtgaacgtgg ctgacaggct acgtgtctcc gggatttcta
     3181 cgggttatga taccgctcaa ctcattgagc agctcatggc ggtggaacgt aggcccgtgc
     3241 tgcttatgca gaaacagcag agccagataa agaccaggtc tgacgcgtgg cgcgatgtgg
     3301 cgagcaggct tgcgaatctg aagagtaagg cggctgcgct caagtccgct tcggtgttca
     3361 acgtcaaatc cgtggcatct tcagcggaca agacagtgtc ggccattgcc gactcgtctg
     3421 cagcgaccgg cacatataag gtatgggtgg accgcctggc tacgtccacc aaggctaccg
     3481 gctcggcgga actcggtcgt gggctcgaca tgggggcaaa actggccgag gcaggtttct
     3541 cgatcacgcc cactgcgggc acttttacta tcaacggggc gcagatacgc atagacgctg
     3601 acactgttat gagcgatggc gtggatggcg cctcgtccaa ctcgattctc ggcaagatca
     3661 actcctcagg agcaggtgtc accgcgagcg tggttgccaa ccgcatggtg ctggcgtcca
     3721 acgacggggt aagccccgta atcgtgggaa gcggcggtga tacgagcaac ttccttaccg
     3781 ccgcccgcct gcttggagcg gctagcggca ctaccgtcac gagccagggc agtctgggcg
     3841 tggccaagac cacagtggcc ctcaccggcg ccaggttggg cgcgccgatc caaagctcgc
     3901 ttctgggcgg ggtggctgaa ggtgagatag ctggcaaact ggcaaccgcg ctcgaaggca
     3961 cggagacagt caccttcacc tatcatggca cggagtacac gaccgaatcc cttgccgcag
     4021 aggacgacat ggccgctgtc gctgccgacc tggaggcaaa gatgaacgct gcggccggtt
     4081 tggctgccgg gactatccgg gtgcgagtga gcggccttga ggagggctcg aaagatagat
     4141 tcgtgataac ggatacccaa acgcctaccg attctgccga gcactcaatc accatcggcg
     4201 cgaacgaagg cgattcgccg acagcgttga ggttggccgc gctagacggg gcggctaaca
     4261 aggggatgtt caagataaac ggtgtggcga tcgagtatga tcgcacccgc gacgccgtga
     4321 acgacataat taacaggatc aacctatcca aggccaacgt gagcgcgtca tacgatctgc
     4381 tgagcgataa gatagttttg acggccaagt acacgggcga gctggccatc tcagtagagg
     4441 acgtaggcgg caacttcctg gaagccgcga gcctgctcga cgccgagcag gagtatgggg
     4501 ccaatgccct gttccgcgtg gaaggcctaa gcggcggagc tcagctctct tcggcgtcca
     4561 acaccgtgaa gggcatcatc agcaacgtga cgcttactct gaagagtgtg agcgatgacc
     4621 cggccactat cactgtctca cagaatgtgg actcagccag tagcgccata aaggcgttcg
     4681 tggaccagta caattcggta atggatctgc ttgccacgca gacggcttac gataaggata
     4741 agaagattgc cggtccccta cagggcgacg gggcagtgcg aacgatccag cagacgcttc
     4801 gtcgccttac cacgacagct gtagacggat tgccccaggg gctcaactct ttgctggcta
     4861 tcggcatatc cacaggcgct gcgggccagg gcgacttcag atcggcgcag ctcaaggtgg
     4921 atgacgccag gctcaaggaa ctccttgccc aggacccgga ggcagtggcg cgggtgtttg
     4981 ccgatgcgga gaacggcatc gcgctcaagt tcgaatcata cctcggcgaa ctgacccgcg
     5041 gcgtcaccgg tgtcctggcc aaaactcagg agattctgag caatcagacc aaatcgatcg
     5101 acaggcggat cgctgatgtg gagaggcgcc tcgaaaagag gagagagggc cttgtgcagc
     5161 agtttaccca gatggagaag gtgctcgctt ccatgcagca gcagcaagcc tggatgagtg
     5221 cacagttcaa ctccatgcag gtgtaatgtg tgcgggaggg cgcgaaagga tgattgactc
     5281 atgatggcca caccctgcaa caggtacatg gagatgcagg tacggactgc gccggctgag
     5341 aacctggtgc taatgttgta cgacggggct cttcggttcg cccgccaggc gcaggttgat
     5401 ctggcggagg gcaggatgca ggacgcccac aacaatctca ctagggccca ggatatcctg
     5461 ggcgagttga tgggttcgct gaacatggac gcaggtgaca tcgcccacaa cctgttcaag
     5521 ctgtacgagt tcattcaata ccgcttgaca gtcgcaaacg tgaaaaagtc caccgaacca
     5581 atcgacgacg ttatacaact gcttggcgat ctcagagcga cgtgggcaga ggcgatacgc
     5641 gacgcgcgcg ggcgtagtgt gaagagcggg ggtcagtgag ttgggccgaa acggacatga
     5701 agctcagctc cgcgagctcg cggagcacat ccacagctgc cgagcggatg agctggaata
     5761 ctaccgcagc attcttgacc tttcacgcgc tcagcgagtg gcgatcgtgg aaggagatac
     5821 cgatactctt gatcaggcga ccgacgagaa gagtcgcttg atatcgctga tagatacgct
     5881 tgatcttaag atagccggcc tgctggatga ggtctcctac gtaacgggcg ttcattctca
     5941 cgcatgtgtc taccctgacg atggccctgc tgcggctact gcctcaactc caatcggggg
     6001 atgtgccctt aacgctagga tagctggtgt gatggcagag atcctggagg ctgagcgtgc
     6061 caatcaagag ctccttgagc aggcgataag cggcgtgcaa gaggagctga aacagattga
     6121 tgcgggcagc aaggctgttc gggcttactg gggccgaggt ggcgacgcgc tgcatccggc
     6181 accagccgta gacgacaatg cgtagcctac ccgtgcgctc gccaggatca cagtgaaagc
     6241 agccggggca gagacgggcc cggagatggc cccgggggtg ggcccggaga tggaaaggcc
     6301 acggctgcag tacagcgata tacccccggg ggtatccaag cacgcatgcc gtcagttttt
     6361 gccgatagtt tcgccgatag gcgcagtttt tgagtacatc gacttgcatg ggaggcgatt
     6421 ggccggcgga ctggcgctga gaagggtgct cttggggtcg atgaggccta gtatgtggca
     6481 tgtagacggc agtacatgcc atcacttgcg cagtatcgcc ggatacatcc aggttatggg
     6541 acatcctgtc accttccgtg cccggtttca cgccgggacc aaccggatac cccccgattt
     6601 tcgcgatttg gaggcggtca ggcggccctc cgaaatcgat gtactcaaaa attgccggta
     6661 tctccgaaac tgttgctgat aattgacgat ataccagctc cagccgtttt ggccacaggg
     6721 atcgcagcta gaccagctaa aaggcctttt ccgcgaggat ggcgccttct accgtcactt
     6781 tgtcctgcca tgcctgctcg cacttgctct tccacttcgc gatgtccacg agtgctgcat
     6841 aggccccgtt cccgggcgcg cagggttttt gatgtgcatg gacgatgtgc atgatgcaca
     6901 ccggcacgat tcgtcgcgtc atggtcttaa tcggctgcgg ccgtggattg ggaaggaaat
     6961 ccggtaatgt ttgaagaaag acggttttga gatcacagac tcggaggcta tcgcgttgtt
     7021 cattggtgat gttagcggcg gcgcgccatg cggcggacat gtgctgattg tcgacgacga
     7081 ggtgaacatg caggtggtgc tggcagaggc ctttgaggat gaaggtcacc gcgtcagcac
     7141 tgcctcaagt ggagcagagg ccatttccgc cattggcgcc tacgattttg accttctgat
     7201 acttgatcta aagcttcccg acatgacagg catcgacgtg ttcagggtgg cccgggagat
     7261 ccgtccgggc attgtggcaa taatgataac agcgtattca tccatcgata cggccatcga
     7321 ggctatgaag atgggcgcgt acgactacat aaccaagccg ttcaacgtag agaagctgct
     7381 gatggtggcg ggcaatgcca ttgaggggag gattctgtcg gcgccccgat tgtcgcactt
     7441 cgccgagctt gccgtggagg atgaggagtt cgggggagta gtgggctcat cacttaagat
     7501 gaggcaactt ctcgacatct gccggagtat tgcacctacg aacgcgacgg ttctgatata
     7561 tggcgaaagc ggcacaggca aggagcttgt ggcgagctac atacacaaga agagtcttcg
     7621 gtcgagcaag cccttcatca gggtgaactg cgcggccata cctgagacct tacttgagtc
     7681 ggagttgttc gggcacgaga agggagcatt cacgcacgcc atatcgcgcc gcctgggccg
     7741 gttcgagctg gcgcacaccg gcacgatatt cctggatgaa gtgggggaga tgtcccccgc
     7801 catgcaggcc aagctgctgc gcgtactgca ggagaaggag ttcgagcgcg tcggcggagt
     7861 cgagactatc aaggtggatg cccgcgtggt ggcggctacc aacca
//
LOCUS       DAOTAC010000096         7896 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_7835_length_7896_cov_4.452111, whole genome shotgun sequence.
ACCESSION   DAOTAC010000096 DAOTAC010000000
VERSION     DAOTAC010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7896
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7835_length_7896_cov_4.452111"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1107
                     /locus_tag="PLM74_05455"
     CDS             <1..1107
                     /locus_tag="PLM74_05455"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HON42566.1"
                     /translation="KGCTSAGLSEPVAIACLQRYAMDQEMARGIRPVTAAEPTGRSVA
                     VIGAGPAGLACSLALAAAGEAVTLYDSSPEPGGVMRYGIPSYRLPREVLDREIGWILG
                     AGVRFVPNTAVGRDVAMADLLREYDAVFIGAGLGEPAVPHIPGANVPGVISAYSFLRA
                     AGEPDEDARQQAVRAVIAQPLGRVVVLGGGNVAVDAACTALRLGAESVTIAYRRTRGE
                     MPAWAEEVEFAEEEGVKLAFLLSPAEVLHADGKARGVRMTRMMLAEDDASGRPRPVPS
                     GEPDETISADTVVLAFGQTANESIFTALPSLGRSDAGMIEADSETGATSVAGVFAGGD
                     AVNGGTTVVQAVSEGLRAAEGIRAYLDSVTPARN"
     gene            1145..2341
                     /gene="preA"
                     /locus_tag="PLM74_05460"
     CDS             1145..2341
                     /gene="preA"
                     /locus_tag="PLM74_05460"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006183.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HON42567.1"
                     /translation="MSVDLSIKFAGIKFENPFTLAASPCTDNAEMVARAFDAGWAAAV
                     LKTTSVEEEVVELVYPMMAGLDFEDKKVIGLQNIDLISERHIDQVEKDIRELKKAYPH
                     KVVGASIMGQKKEHWQTLVRRLEAAGVDFIECSFSCPHGMPERGMGSTIGQDAELTER
                     TARWVKEAASRVPVSIKVTPLVADLPHIACAVQKSGADAITAINTVKCLIGVDIDNFV
                     PYPNVDGRSSYGGYSGAAIKPISLRCVAEIAKTVDMPISATGGITTWRDAVEFLLCGA
                     RNLQVCTAAMKHGFRVIEDLTDGLSVYLEDKGMSSVEELVGLALPNIVEHSHLSREYK
                     PVSKINLETCVKCDVCYIACQDGGHQAITLDEKRLPHVDEEKCVGCAMCASVCPVFEC
                     IQMAPR"
     gene            2380..3690
                     /locus_tag="PLM74_05465"
     CDS             2380..3690
                     /locus_tag="PLM74_05465"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HON42568.1"
                     /translation="MDTFRALTARSAREAVEMLAAEPGAVLAAGCTDLLVKMKDGKVR
                     PSAVISIDRATDMSSIADTGRTLWVGAGCTHASISRSPLIAEKAPLLAAASAAVGSPQ
                     IRNRGTLGGNIATASPAGDTVPALIAMGARIVALGLAGQRAIEAEQFFLGPGRSALGS
                     REVIVGVEIPCDEPGDVWAFRKLGGRRAMSIAIASVAVHAHLESGVLSRVAVALGSCA
                     PVVVRARKLEEALEGRQVTADLLAKVCPLAREAASPITDIRGSREYRLAMVEALAYQA
                     LYAAVWPDEVSEPRAKWESSAGGAQGEHECCCHGRPDRSRADASGADGALGADGASSP
                     CVVHRPGEPQSASASREAEATGSGSDSIRVHVVAFGELACRVEKADVTLNLPRGSTVA
                     DLYRRIGLANSSYVMALVNHRREFDDKTLSDGDEIALMHPVGGG"
     gene            3704..6007
                     /locus_tag="PLM74_05470"
     CDS             3704..6007
                     /locus_tag="PLM74_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018704998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HON42569.1"
                     /translation="MQEFKWVGRPVMRVDARGKVTGGQKYMGDLRFPGMAYGRVLRSK
                     YPHARIKSIDTARAESAPGVVKVLTWRDVGGLNGYGIAIPDQPVLCSDKVRYMGDAVA
                     LVIAESPEQAEQALAVVNVEYEVLPVVDDPEAAMLPDSPRVHESGNVHMQTHVENGDV
                     EQGFAASDVIVEGTYRLPRQMHAFMEPEQGVGVPGPNGEVTVYCGAQHPYRDQMQVSR
                     ALGIKPSKIRVVTSPTGGAFGGKDEITLQILLAMGALATGRPVQIVLSREESTVAGWK
                     RHPMTIYMKTGARRDGTLLAHQVRLVSDTGAYASLGGPVTNLALEGCTGVYRIPAIVM
                     DGYCVYTNNGVAGAFRGFGQLQANFAIEHQMDRLASELGIDPVEIRLRNALRPGDPNP
                     LGHTMVESVGVIPTIEAAARCDIWARREEYCRQSSAPWRKRGVGFATEMQGTGLGVGL
                     PDYGAALIRLLGDGTWTVGLSCPEIGQGNSTTYAQMAAEGLEVHVQDVEVFVGDTGLG
                     PDSGSCTASRSVYTGGNAIFRAATQMRDRLRGEAAGVFGCDPEEVVYSEGVCRRVDGT
                     ASATVQEIAARAAAEGREITADGAFIWPVSKVQIPGAVGLPHIINTYITHVALVEVDE
                     MTGETSVLKAFAAIDCGRVINPQGLRGQSEGGMVMGMGYALMEDTIIEGGKTLTPNFS
                     TYIIPTSIDAPDMDTVTCEVLEPSGPYGAKGVGEAVMVPIAPAITIGIDRAVGARVTH
                     APATSERVFHGMRSRDGGRGVGEGSTA"
     gene            6004..>7896
                     /locus_tag="PLM74_05475"
     CDS             6004..>7896
                     /locus_tag="PLM74_05475"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HON42570.1"
                     /translation="MNCKDQMWHPVGARAPRVDGADKVAGRALFGADLTFDNMLWGQP
                     VRTRYPHARILAIDTSKAEAIPGVVRVLTANDVPGSRTFGVVVPHQPLLCWDRVRFMG
                     DAVAVVLAESREAAQMAACSVQVEYEVLPSVFDPEEAIRPGAPQLHDEYPANVAAHHV
                     VRKGDVERGFAEADVILERDYTTQLIEHAYIEPEAAVALPGPGGGVTVYGSIQNPFSC
                     RRAVAGVLGLPLAKVRIVQSHMGGSFGGKDEVMSAMCARVALGAQLTGRPVKIVNTRE
                     ESLVESYKRHPYRMHYKIGVRRDGHITAMEVRMLADAGGYASQSAFVTWRSTVQATGP
                     YVVENVKTDIYAAFTNNTYTGAMRGFGSPQAIFANESLMDEVALELGMDPVQLRMVNG
                     FEGGSVTATGHKLDEHTVSLKEVVGKATEAAGWGEKRARLPLENQGRAIKRGIGMACS
                     FRGCALGAEGVDAAAAIVSVQTDGSVLVFSGLAENGQGLKTIFAQIAATELGVTLDRV
                     VFMETDTSTVPDSGPTVASRSTIMGGSAVRIAAQKARDALFEGAAAKLGVGLEQLAAG
                     NNEIYVASDPVRRMHFDDAVKIAWNEGRLMAQFGWYGAPHVNWDEHTGQGKAYFTYVY
                     ACQVAEV"
BASE COUNT         1506 a         2150 c         2808 g         1432 t
ORIGIN      
        1 aagggatgca caagcgcagg gctgtctgag cctgtggcta ttgcttgcct gcagcggtat
       61 gccatggacc aggagatggc ccgcggaatc cgcccggtga cggcagcaga gcccaccgga
      121 aggagcgtcg ctgtgatcgg ggcaggccct gcggggcttg catgcagcct ggcgctggct
      181 gcagcggggg aagcggtcac cttgtatgac agctcgcctg agccgggagg ggtcatgcgg
      241 tatgggatac catcatatcg tctgccgcgg gaggtgctgg atcgagagat cggctggata
      301 ctcggcgcag gcgtgcgatt tgtgcccaat accgcagtcg ggcgggatgt cgccatggct
      361 gacttgctgc gcgagtatga cgcggttttc atcggggccg ggctgggtga acctgcagtt
      421 ccccacattc cgggggccaa tgtgcccggc gtcatatcag cctattcatt cctgcgggcc
      481 gcaggcgaac cggacgagga cgcgcgccag caggctgtca gagccgttat cgcgcaacct
      541 ctgggtcgcg tggtcgtgct gggcggaggt aatgtggccg tcgatgcggc ttgcactgcg
      601 ctccggctgg gagccgagtc tgtcaccata gcctaccggc gcacgcgtgg cgagatgccg
      661 gcgtgggcgg aggaggtcga gttcgccgag gaagagggcg ttaagctcgc tttcctgctg
      721 tcgcccgcgg aggtgttgca tgccgacggc aaggcccgag gcgtgcgaat gaccaggatg
      781 atgctggctg aagatgacgc gtcgggccga ccgcgtccgg tgccttcggg cgagcccgat
      841 gagacgatca gcgccgatac tgtagtgttg gcattcgggc agacggcgaa cgagtccata
      901 ttcaccgctc ttccgtcgct tgggcgttcg gacgctggga tgatcgaggc agattccgaa
      961 accggcgcga cgtctgtcgc aggcgttttt gccggcggag acgcagtcaa cggaggaact
     1021 acggtggtgc aggcagtgag cgagggtctt cgggccgcgg aggggatccg agcatatctc
     1081 gattcagtga ctcccgcgcg caactgactt ttgatcggaa ttatctatta atggagggat
     1141 cggagtgtcg gtggatcttt ctatcaagtt cgcagggatt aagttcgaga acccgtttac
     1201 gctggcagcc tctccatgca ctgacaatgc ggagatggtg gctagggcat ttgacgccgg
     1261 atgggcggcg gcagtgctca agacgacatc ggtggaagaa gaagttgttg agctggtcta
     1321 ccccatgatg gccgggctcg acttcgagga caagaaagtc atcggcttgc agaacatcga
     1381 ccttatctcg gagcgccaca tcgaccaggt ggagaaggac attcgagagc tgaagaaggc
     1441 atatccccac aaggtggtcg gggcgagcat catgggccag aagaaggagc actggcagac
     1501 cctggtgagg aggctcgaag ccgcgggcgt ggacttcatc gagtgcagtt tttcgtgccc
     1561 gcatggcatg cccgagcgcg gcatgggctc cacaatcggc caggacgccg agctcacgga
     1621 gcgcacagcc agatgggtga aggaggctgc ctcgcgcgtg cccgtgtcga ttaaggtaac
     1681 ccctctggtg gctgatctgc cgcacatcgc ctgcgcggtg caaaagtccg gggcggacgc
     1741 cataaccgcc atcaacacag tgaaatgcct cataggtgtg gatatcgaca atttcgtccc
     1801 ttacccgaac gttgacggga ggtcttcata cggaggctac tccggagcgg ccatcaagcc
     1861 catctctttg cgatgcgtgg cggagatagc aaagactgta gatatgccta tctcggccac
     1921 aggcggcatc acaacatggc gcgacgctgt ggagtttctg ctgtgcggcg caaggaatct
     1981 gcaggtttgc acggcggcga tgaagcatgg gttcagggtc atcgaggatc tcaccgatgg
     2041 cttgtcggtg tacctagaag acaagggtat gtcgtcagtg gaagagcttg tgggactggc
     2101 gcttccgaac atcgtggaac actcacacct ttcgcgggag tacaagccgg tgtccaagat
     2161 caacctggag acctgcgtca agtgcgacgt ctgctacatc gcgtgtcagg acggcgggca
     2221 ccaggcaatt accctcgatg agaagcggct accgcacgtg gatgaggaga agtgcgtagg
     2281 gtgcgccatg tgcgccagcg tatgtcctgt gttcgagtgc atccagatgg cgcccaggta
     2341 ggctgcatat cgcttcgtcg agggaggtga gcggagcaga tggatacgtt cagagcattg
     2401 acagctcgga gcgcccgcga ggctgtggag atgttggcgg ccgagcccgg ggcggtactg
     2461 gcggcgggct gcaccgacct tctggtgaag atgaaggacg ggaaggttcg accgtctgcg
     2521 gtgatcagca ttgaccgcgc gaccgatatg tcgtcaattg cagatacagg ccgtacgctc
     2581 tgggtggggg cggggtgcac acatgcgagc atatcccgtt cgccgctgat cgcggaaaaa
     2641 gcaccgctgc ttgcggcggc gagcgcagcg gtaggttctc cccagatcag gaaccgcggc
     2701 acgttgggcg gcaatatcgc aactgcctcc ccggcaggcg acacggttcc ggcgctcatc
     2761 gccatgggcg cccggatagt agccctgggg ctcgcggggc agcgggcgat cgaggccgaa
     2821 cagttcttcc taggtccggg ccggtcggcg ctggggtcgc gggaggtaat cgtgggggtc
     2881 gagatccctt gcgacgagcc gggagatgtg tgggccttcc gaaagcttgg ggggcgacgc
     2941 gccatgtcca ttgccatcgc cagtgtggct gtgcatgccc atcttgagag cggcgtgctg
     3001 agccgtgtcg cggtggcgct cgggtcttgc gcacctgttg ttgtgcgcgc gcgcaagctg
     3061 gaggaagcgc tggaaggccg gcaggtcacc gctgatttgc tcgcaaaggt gtgtcccctg
     3121 gctcgtgagg ccgcctcgcc gataaccgac atacgcgggt cgcgcgaata caggctggcc
     3181 atggtggagg ccctggctta tcaggcgctc tacgcggccg tctggcccga tgaggtgagc
     3241 gaaccgcgag ccaaatggga atcttctgca ggcggggccc agggcgagca tgagtgctgt
     3301 tgccatggcc gaccggaccg atcgcgggcc gacgcatcag gtgcggacgg cgcgctaggt
     3361 gcggacggcg cgtctagtcc gtgcgtcgtc catcgaccgg gtgaaccgca atctgcatcg
     3421 gcgagccggg aggcggaggc cacgggcagc ggctctgact cgattcgagt gcatgtcgtg
     3481 gccttcggcg aactagcctg ccgggtggaa aaggccgatg tgactctgaa tcttccgcgg
     3541 gggtcgacgg tggcagactt gtaccgccgt atcgggctgg ccaattccag ctacgtgatg
     3601 gcactggtca accatcgccg cgagttcgac gataagactc tgtccgacgg ggacgagatc
     3661 gcattgatgc atcccgtcgg cggcggatag tgagggacgc gccatgcagg aattcaaatg
     3721 ggtaggcaga cctgtaatgc gggtggatgc acgcggcaag gtcaccggcg ggcagaagta
     3781 catgggcgat ttgagattcc ccgggatggc ctacgggcgc gtgctcagaa gcaagtatcc
     3841 tcatgcccgc atcaaatcga tcgatactgc cagggctgag agcgcgccag gagtggtgaa
     3901 ggtgcttacg tggcgcgacg tgggagggct caacggttac gggatagcca tacccgacca
     3961 gccggtattg tgcagcgaca aggtcaggta catgggtgat gcggtggctt tggtgatagc
     4021 cgaaagcccc gagcaagccg agcaggcgct ggcggttgtg aatgtggaat atgaggtctt
     4081 gcctgttgtg gacgatcccg aggcggccat gttgcccgat tcgcccaggg tgcatgagtc
     4141 ggggaatgtg cacatgcaga cccacgtcga gaatggtgat gtagaacagg ggtttgccgc
     4201 atccgacgtg atcgtggaag ggacctacag gttgcctcgg cagatgcatg ccttcatgga
     4261 gccggaacag ggagtgggag tgccggggcc aaacggcgag gtcacggtgt actgcggagc
     4321 ccaacacccg taccgtgatc agatgcaggt ttctagggcg ctaggcataa agccatccaa
     4381 gatccgggtt gtcacaagcc ctacgggcgg cgcgttcgga ggcaaggatg agatcactct
     4441 ccagattctg ctggccatgg gcgccctggc aaccggtcgc cctgtgcaga tagtgctgtc
     4501 ccgagaggag tcgacggttg caggctggaa gcgccatcca atgaccatct acatgaagac
     4561 cggtgcacgg cgcgacggca cgctccttgc gcaccaggtc cgcctggtgt cggacaccgg
     4621 ggcatatgcg tcgctcggcg ggccggtgac caacctggcc ctcgaaggat gtaccggcgt
     4681 ctatcgaatt ccggcaatcg tgatggatgg ctactgtgtg tacacgaata atggcgttgc
     4741 gggcgccttc cgcggcttcg gccagcttca ggcgaatttc gccatagagc atcagatgga
     4801 ccggctggcc tcggaacttg gcatcgaccc tgtcgagatc aggctgcgaa acgccctgcg
     4861 ccccggggat ccgaatccgc tcgggcacac catggtggag tcggtaggag tcattcccac
     4921 aatagaggct gctgctcggt gtgacatctg ggcccggcgc gaggagtact gccggcagtc
     4981 gtcggcgccc tggcgcaagc gtggagtggg gttcgccact gagatgcagg gaacaggcct
     5041 aggagtgggg ctgcctgact acggggctgc cctcatcagg ctgttgggcg acggcacatg
     5101 gaccgtgggg ctttcgtgcc cggaaatcgg ccagggcaat tccacgacat atgctcagat
     5161 ggccgcagag ggccttgaag tgcacgttca ggacgtcgag gtcttcgtgg gcgacacagg
     5221 gcttgggcct gattcgggat cgtgcacggc gtcgcggtcg gtgtacaccg ggggcaacgc
     5281 cattttccgg gcagctacgc agatgcgtga caggctgcgc ggcgaggcag cgggcgtctt
     5341 cggctgcgat ccggaagagg ttgtgtattc agagggcgtc tgccggcgag ttgatgggac
     5401 ggctagcgca actgtgcagg agatcgcggc gcgggcggct gccgagggcc gcgagatcac
     5461 ggcggatggc gcgtttatct ggccggtttc caaggtgcag ataccggggg cagtggggct
     5521 tccgcacatc atcaacacat acataaccca tgttgccctg gtggaagtgg acgagatgac
     5581 cggcgagacg agtgtcctca aggctttcgc ggcaatcgac tgcgggcgcg tcatcaatcc
     5641 gcagggcctg cgggggcagt cagagggcgg aatggtgatg gggatgggct atgccctgat
     5701 ggaagacacg ataatcgagg gtggcaagac cctgaccccg aacttcagca catacatcat
     5761 ccccaccagt atcgacgcac ccgacatgga caccgtcaca tgcgaagtac tggaaccttc
     5821 cgggccgtac ggcgccaagg gagtgggcga agcggtgatg gtgccgatag cgcccgcgat
     5881 aactataggg atcgacagag ccgtgggggc cagagtcacc cacgcgccgg ctacgagcga
     5941 gcgggtgttc catgggatgc ggagccgcga cggcggtcgg ggcgtagggg agggatcgac
     6001 tgcatgaact gtaaagacca aatgtggcat ccagttggag cgcgcgcgcc tcgggttgac
     6061 ggcgccgaca aggtggcagg gcgcgctctg ttcggcgccg acctgacctt cgacaatatg
     6121 ctgtggggcc aacctgtgag gactcggtat ccacatgcca ggatactagc tatcgatacg
     6181 tcgaaagcgg aggccatacc cggagtcgtt cgggtgctca ctgcgaatga tgtgcccggc
     6241 tcgcggacct ttggcgtagt agtgccgcac caacccctgt tgtgctggga tcgagtcagg
     6301 ttcatgggag acgctgtagc tgtggtgctc gccgaatcgc gggaggcagc gcagatggcg
     6361 gcatgctctg tgcaggtgga gtatgaggtt cttccgtcgg tgttcgaccc ggaggaggcc
     6421 atccggcccg gggcacctca gcttcacgac gagtacccgg cgaatgtcgc tgctcaccat
     6481 gtcgtgcgca agggcgatgt ggagcgaggg tttgccgagg ccgacgtgat cctcgagcgc
     6541 gactacacta ctcagctcat cgagcacgca tacatcgagc ccgaggcggc ggtagctctg
     6601 cccggcccgg gcggcggcgt gacggtgtac ggttccattc agaacccctt ctcatgcagg
     6661 cgggcggtgg caggcgtgct tggactgccg ctggctaagg tgaggatagt ccagagccac
     6721 atgggcggtt cgttcggagg caaggacgaa gtgatgtcgg ccatgtgcgc ccgcgtggcc
     6781 ctcggcgccc agctcacggg aagacctgtc aagatagtca acacccgtga agagtctctg
     6841 gtggagtcat acaagcgtca tccatatagg atgcattaca agatcggggt gcgacgcgac
     6901 gggcacatca ccgccatgga agttcgcatg ctggccgatg cgggcggata cgcatcgcag
     6961 agcgccttcg tgacgtggcg ttccacagtt caggcgacag gaccttacgt tgtggagaac
     7021 gtgaaaaccg acatctatgc ggcgttcacc aacaacacct acaccggcgc tatgcggggt
     7081 ttcgggtctc cgcaggcgat tttcgccaat gaatcgctca tggacgaagt ggcgctggag
     7141 cttgggatgg atcctgttca gctcaggatg gtgaacggct tcgagggcgg atcagtcacg
     7201 gccacagggc acaagctgga cgagcatacg gtttcgctga aagaggtagt gggtaaggcc
     7261 accgaagctg cggggtgggg cgaaaaacgc gcgcggctcc cactggagaa ccagggcagg
     7321 gcgatcaagc gcggaatagg catggcgtgc agtttccgcg gatgcgcgct gggggcggaa
     7381 ggcgtagacg ccgccgcggc catagtgtcg gtgcagacag acggctcagt gctggtcttt
     7441 tcggggcttg cagagaatgg gcaggggctg aagacgatat tcgcgcagat agccgcgaca
     7501 gagctaggcg tgacgctgga ccgggtggtc ttcatggaga ccgacacgtc caccgtgccc
     7561 gacagcggcc ctacggtggc gtcgcgcagc accattatgg gcgggagcgc ggtgcgcata
     7621 gccgctcaga aagcgcgtga tgctctgttt gagggcgcgg cggcgaagct cggagtcggc
     7681 ctggagcagc ttgctgcggg taacaacgag atatacgtgg ctagcgatcc tgtgaggcgc
     7741 atgcatttcg atgacgccgt gaagatagcg tggaacgaag gccggctcat ggcccagttc
     7801 gggtggtacg gcgcgcctca tgtcaactgg gacgaacaca ctgggcaggg gaaggcctac
     7861 ttcacctatg tctacgcatg ccaggtggcg gaggtt
//
LOCUS       DAOTAC010000097         7861 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_7882_length_7861_cov_4.829106, whole genome shotgun sequence.
ACCESSION   DAOTAC010000097 DAOTAC010000000
VERSION     DAOTAC010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7861
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7882_length_7861_cov_4.829106"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="PLM74_05480"
     CDS             complement(<1..766)
                     /locus_tag="PLM74_05480"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HON42571.1"
                     /translation="MISLKDVQLRRAGRLVLDIADLTVGRGESVAVIGPNGAGKSTLL
                     QVMACLLPLDSGHLTVLGERVERGANPIHIRRRTAMVLQRACLLNASVYENVALGLRI
                     RKTPETAIRERVINALSMFRVEHLARRPARALSGGESQRVSLARAFALEPEILFLDEP
                     FNALDLATRTALLREVGEAVRASGATTIFVTHDVTEIPFLSDRTIVMNEGRVVADGSI
                     RRVLADDTRSALSELLRSASWLCGKDGDDGCDFDAGF"
     gene            complement(763..1467)
                     /locus_tag="PLM74_05485"
     CDS             complement(763..1467)
                     /locus_tag="PLM74_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HON42572.1"
                     /translation="MDLIAEGITQAFWLLVKGDRGVFQIALLTLKVSGLATLISLATG
                     VPLGTALALGRFRGRDAVASIVNTGMGLPPVVVGLWVSIVLWRYGPLGSLRLMYTPTA
                     MVIAQTLIALPIITGLTMSGIGQLDPGIRVQILSLGATRWQLIWLLVREARLAILAAV
                     MAGFGRVIAEVGASMMVGGNVVGQTRVLTTATSMEVGRGNFGTAIALSLILMGLTLAI
                     TSWLTHLQQKGRQTAQ"
     gene            complement(1475..2338)
                     /locus_tag="PLM74_05490"
     CDS             complement(1475..2338)
                     /locus_tag="PLM74_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HON42573.1"
                     /translation="MLSRSSVRSVTRILLTVLLLAILTITASAAAPAREVILATTTST
                     ADTGLLDALIPIFEKKTGYIVKTIAVGTGQALALGSRGEADVLLTHAPSSELPLVKSG
                     DVVERRLVMHNDFVIAGPAGDPAKVRGLKLAADALSRIQSSQSVFVSRGDDSGTHKKE
                     KEIWGKLGVKPSGKWYVEAGAGMAHTLNIASEKEGYTLTDRGTYLALKKNLALDILVE
                     GDAILLNIYHVMQVNPEKFSKVNGPGGLAFVEFMVSPEVQNIISTFGVDKYGDPLFFP
                     DAGKNVDDLGK"
     regulatory      complement(2331..2550)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            complement(2904..3533)
                     /locus_tag="PLM74_05495"
     CDS             complement(2904..3533)
                     /locus_tag="PLM74_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006100976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42574.1"
                     /translation="MGTVAYSVRADVVDIRTDLPRHDDTLLVDTNVWYWVTYSRASQA
                     SRSPSRYQVRDYPLYVKNAMAAGARVCCCCLSFAELAYLIENTERDIKSVMSGSPIEP
                     KVFRHNHPLDRQRVVSEISAAWAQVRSMSSSVEAVVNPGLINAAITRLKTDAMDGYDI
                     LMLEASRADGTCQILTDDGDFATVSGIRVHTSNRNVIRAARDAGKLLVR"
     gene            complement(3514..3882)
                     /locus_tag="PLM74_05500"
     CDS             complement(3514..3882)
                     /locus_tag="PLM74_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015159721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS-like domain-containing protein"
                     /protein_id="HON42575.1"
                     /translation="MHMVSVHDVSGVNCITLEDGEKVYELVHPLLSKGVPVTLDFSGV
                     RVFASSFFNSAVGQLFRDLDSDTLNRLLLIVNLESDGTTVLRRVIENSRQYYADPRMR
                     EAVDSVMDRQSEDADGNCSL"
     gene            complement(3895..4773)
                     /locus_tag="PLM74_05505"
     CDS             complement(3895..4773)
                     /locus_tag="PLM74_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HON42576.1"
                     /translation="MSEIRIYVPTANDDAVDFLKLFRLYSNTLAVASNIVFDFSQCSF
                     LRQNAVVFMGGLARSVQQRGMQVRFDVSTMRDRLKTNLSQNGFLRAFGFGIGPWAGNS
                     ARYREDVVPNADDIVEYISDQWLRDEWVSFSPRLRNAITGRIWEIFANAFEHSESEVG
                     VVSCGQLYPRLREMCFTIADFGIGIPGKVRRFYGDPALKDSACLKWAFKRGTTTSRGT
                     VGRGLGLDVLKEFIGLNSGRLEVFSGCGWGCIQPGGEQYTDLGSGFDGTLVNISLRCD
                     EAYYRFKHEDDETVVF"
     gene            complement(5034..5945)
                     /locus_tag="PLM74_05510"
     CDS             complement(5034..5945)
                     /locus_tag="PLM74_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42577.1"
                     /translation="MANYDVVSTDAGRVIAALGISVISGGGLAPLRDVGFVVGVAGAG
                     DVWLHPSVLPTAERQANANLAITHMPSSMAGKPITAVRFEAADDVSRRVSVFDESTGI
                     LVYRIQTLGSLDTARQNSMVMEFISVRQTEPAWAGGSVPAWAQVGTQLVYEGSTMVNV
                     GYGSSVIPMPTTMLARIVHVESTLAVVQVEQYLSGMKSGEMHSVTSTVVPLGGIFLPP
                     EALAVLKPGQVLDRDAITGVTVSVPENPVYIDGQPLLALLFEGASFQRLFAYDRQSGT
                     LVYLMELAREGIATTRREFKLTGVRRG"
     gene            complement(6015..6854)
                     /locus_tag="PLM74_05515"
     CDS             complement(6015..6854)
                     /locus_tag="PLM74_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HON42578.1"
                     /translation="MSRSKVADILILLCLIGACALVLIPLAFMFTASTMPAIDIMKLP
                     YPWFSDHIHWQNFWNGLRGPEGDFILLRNTLNSLIVAVTVTASTVLLSSMTGYGLAKF
                     EFKGRNVVFMTIMATMMIPFEAIMIPLYMVVMGMGLQDSYAGLIVPFLTSAFGIFLMR
                     QFLLTFPDEVLDAARIDGASEPRIFASIVLPNAGPAIATLAVLTFRSQWDSLLWPLLA
                     VQSEEMKTIPLYITKFMAETHSDEGIMMAIAAMASLPMFILFFTMSKYFISREGLYST
                     TKG"
     gene            complement(6962..7849)
                     /locus_tag="PLM74_05520"
     CDS             complement(6962..7849)
                     /locus_tag="PLM74_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012002183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HON42579.1"
                     /translation="MEQKKARWGWIFVTPSLVFFAVFSFYPIFNAFYLSFFRKNLLSL
                     KPPTFIGFGNYTYLFSSLLFKESVVNTAIFTFGTFVPLLVLSLILAAFIMSRKRFQKF
                     YQMVLYSPAALSSVVAAMIWLLIFDPRGLANQGLNLLFGTSGVNYHWLSNDAMLRLST
                     IIVYVWKYVGYFTVIFVAGIGAVPHTLHEAALIDGADRWQDFWHITFPLLKPTTLLVS
                     VMAMIQCLKTFSTQYLFTTAGAPRQPINVLTLNIYYTAIKDHSIGRASAMSVLLFLVM
                     LVFTWLQLRLSRTEEVTYM"
BASE COUNT         1742 a         2339 c         2158 g         1622 t
ORIGIN      
        1 aaaaaccggc gtcgaagtca cagccatcat caccgtcctt gccgcaaagc cacgaggcag
       61 atcgcaacag ctccgacaga gcgctgcgcg tgtcgtctgc aagcacgcgg cggatgctgc
      121 catctgccac aacgcgccct tcgttcataa caatcgtcct atcgcttagg aacgggatct
      181 ccgtaacatc gtgagtcacg aatatggtag tcgcgccgga ggcgcgcacc gcttcaccca
      241 cctcgcgcag gagagctgtt ctggtcgcaa ggtctagcgc gttgaaaggc tcgtctagga
      301 atagtatctc cggttcaagc gcgaatgctc tggccaagct gacccgctgg ctctcgccac
      361 ctgagagcgc acgagcgggc cgtcgcgcca aatgctctac gcggaacatc gacaacgcgt
      421 tgatcacgcg ctcacgaatt gcggtttcgg gtgtctttcg gatcctcagg ccgagcgcca
      481 cgttttcgta cactgaggcg ttgagcaggc acgcacgctg gagtaccatg gctgtgcgcc
      541 gccttatgtg aatcgggtta gcaccgcgct caacgcgctc gcccaaaaca gtgagatggc
      601 ccgaatcaag cggaagcaaa caggccatta cctgaagcag tgtgctcttg cctgcgccgt
      661 tgggacctat gacagccaca gactcgcctc gcccgactgt caggtcggcg atatcaagca
      721 caagccgtcc ggcgcgccga agctgtacgt ctttgaggct gatcattgtg ccgtctgccg
      781 acctttctgc tgtaggtgcg tgagccagga tgtaatggcg agcgtgaggc ccatcagtat
      841 cagcgacagc gcgatggccg tgccgaagtt gccccgcccg acttccatgg acgtagccgt
      901 ggtaagcacg cgggtctgcc ccacaacgtt tccaccgacc atcatcgagg ctccgacttc
      961 agctatcact cggccaaacc ccgccattac cgccgccaga atagcaagcc ttgcttcgcg
     1021 cacgagcagc catatgagct gccagcgcgt ggcgccgagc gacaggatct gcacgcgaat
     1081 gcctgggtcg agctggccta tgcccgacat ggtaagcccg gtgatgatgg gcagggctat
     1141 gagagtctgg gctatcacca tagctgtagg agtgtacatg aggcgcaacg agccaagggg
     1201 tccgtatctc cacaaaacga tgctaaccca gaggcccact accaccgggg gcagtcccat
     1261 gccggtattg actatgctgg ctaccgcatc acgtccgcga aaccttccaa gggcgagggc
     1321 cgtgccgagc ggaacgcctg ttgccaggct gatcagggtg gccaggcccg acaccttcaa
     1381 ggtaagcagg gcgatctgga atacaccgcg atcgcccttc accagtagcc agaaggcctg
     1441 cgtaatacct tcggcaatga ggtccaaggt ctacctactt tccgagatcg tccacgttct
     1501 tgccggcatc agggaagaac agagggtctc cgtacttgtc cacgccgaaa gtgctaatga
     1561 tattctgcac ttcaggcgat accatgaatt cgacaaaagc cagcccgcca gggccattca
     1621 cctttgagaa cttctcgggg ttcacctgca tcacgtggta gatattgagc aagatcgcat
     1681 cgccctcaac tagtatatcg agggccaggt tcttcttgag ggcaaggtaa gtaccgcggt
     1741 cggtgagtgt ataaccctct ttttccgatg caatattgag cgtatgggcc atgccggcgc
     1801 cggcttccac ataccacttg ccggaaggct tcacgcccag cttgccccag atctccttct
     1861 ccttcttgtg ggtgcccgaa tcgtcgccgc gcgacacgaa caccgactgg ctgctctgaa
     1921 ttctggacag cgcatcagca gccaacttca gcccgcgcac cttggcggga tcgcctgccg
     1981 ggcctgcgat gacgaaatcg ttgtgcatca ccagcctgcg ctcaacaaca tcgccactct
     2041 tcaccagcgg gagttctgag ctgggcgcat gcgtaagcag cacgtccgcc tcgccgcgac
     2101 tgccgagagc cagggcttga ccggttccga cggcaatcgt ctttacaatg tagcccgtct
     2161 tcttctcgaa aatggggatc agagcgtcaa gtaggccggt gtcggctgta ctagtggtcg
     2221 tggccaggat tacctcgcgg gcgggggctg cggcgctcgc agtgatggtg aggatcgcga
     2281 gcaacaggac ggtaagtagg atacgcgtga ctgatctgac agacgatctg gacaacatta
     2341 atacatctcc tttccaaacg tgggaggcct gccagtttcc cgacagaccc tcgagccgcc
     2401 ggccgtggta cacattgact gcaatacaca tcgccacgct cgcgacaacg cgagccgcgg
     2461 cgcaacgcag catggagcag cgccggcgac taccggccag gcgccatccc gcgtggggta
     2521 aggcgcgctg actcggaggg tagcccaata tgtagttgtg ggcgaggcac ggtgcctgct
     2581 aatgcctctg ttcatagtct agacgggctg ggagggcaag tcaaccttgc gttgcaactg
     2641 caactcgcga ttgcagctca ccgctcgcct gagtgagtgc aacgattgct gctctcacga
     2701 gataccttca ttccctgacg atagtacggg tctatgtgct cagcttctgc gtacagtctg
     2761 tgggccagcg gggggctgag actatgtagg gcgcatggga aaaccgggag aaggggaggg
     2821 cgcttgagaa gtcgggctgg ccccagctac tgtccccgca taggcgaaga atcgagtccg
     2881 agtagccatg ctttcctcgc gcttcaccta acaagcagct tgcccgcatc acgcgcagcg
     2941 cgaattacat tacgattcga cgtgtgtacc cttatgcccg aaacagtcgc gaagtcaccg
     3001 tcatcagtca aaatctggca tgtaccgtca gcgcgtgacg cctccagcat aaggatatcg
     3061 tatccgtcca tggcgtctgt ctttagtctc gttattgctg cattgatgag ccccgggtta
     3121 actaccgcct caacactcga cgacatggaa cgcacctggg cccacgccgc actgatttcg
     3181 ctaacgactc gctgccgatc aagcgggtga ttgtggcgga agaccttcgg ttcgatggga
     3241 ctgccgctca ttacggactt gatgtcgcgt tcagtattct cgataaggta cgccaattca
     3301 gcaaacgaca gacagcagca acatactctg gcgccggctg ccatcgcgtt cttcacgtag
     3361 agtgggtaat ctctcacctg gtatcgagat ggagatctgg atgcttggct ggctcgtgaa
     3421 taggtaaccc aataccagac gttagtgtcc accaatagcg tgtcgtcgtg cctgggcaga
     3481 tcggttctga tgtcgactac atcggccctg acactatagg ctacagttcc catcagcatc
     3541 ctccgactgc ctatccatga cactatcaac agcctcgcgc attctgggat cagcataata
     3601 ctgcctagag ttctcgatta ctctgcgcag cactgttgtg ccatcgcttt ctagattgac
     3661 gatcaaaagg agccggttca gggtatccga atccaaatcc ctgaaaagct gtcccactgc
     3721 ggaattgaag aaggaagatg cgaaaaccct gactcccgaa aagtccagtg tgacggggac
     3781 tcccttcgac aacaaggggt gtaccagttc atacaccttt tctccatctt ccagcgttat
     3841 acagttcacg cccgacacgt cgtggacgct caccatatgc atgctcatcc tcctctagaa
     3901 taccacggtc tcatcgtcct cgtgtttgaa gcggtagtac gcctcatcgc agcggagaga
     3961 aatgttcacc agagtaccgt cgaagcccga cccgagatca gtgtactgtt ctccgcccgg
     4021 ttgtatgcag ccccagccgc atccagagaa aacctcaagc ctaccgctat tgaggccaat
     4081 gaactctttc agcacgtcca gaccaagccc tcgccccact gtgccgcggc tagttgttgt
     4141 accacgcttg aaggcccact taaggcatgc ggagtccttg agggccgggt ctccatagaa
     4201 cctgcgaacc tttcctggta tacctatgcc gaaatctgcg atagtgaaac acatttcgcg
     4261 aagcctgggg tatagttgcc cacagctaac gactccaacc tctgattcgg aatgctcgaa
     4321 agcgttggcg aatatctccc atattctgcc cgtgatagcg ttcctgagcc tcgggctgaa
     4381 actgacccac tcatctctta gccattgatc cgaaatgtac tccacgatgt cgtcggcatt
     4441 gggcacaacg tcttcccgat agcgagctga gttaccggcc cagggaccaa taccaaaacc
     4501 aaatgctctc aagaatccgt tctgcgacag attcgttttc agccggtccc gcatggtgga
     4561 cacatcgaat ctgacttgca tacctctctg ctgtaccgag cgcgcaagcc ctcccatgaa
     4621 gaccacagcg ttctggcgca ggaagctgca ctgcgagaaa tcgaaaacga tattcgaagc
     4681 tacggccagc gtgttactgt agaggcggaa cagcttcaag aagtcgacgg catcatcgtt
     4741 cgccgtagga acatatatgc ggatctcgct cacgtgctgg acgcctcccc gctgatgggc
     4801 acgctactag ggcactgcac cattctgctg aacgcgagac aacttcagta taccactacc
     4861 attactggca cgtaaagggt aatgctgccc ttcatcatgg tgtgcggttc gtttctgcta
     4921 gggttcgtcc tgtcacatcc cgcgggtgct ttacggatac taggaggacc acacggccaa
     4981 gaaaacgagg cacgtgggaa gctagtcccc ggtgctgcgc ctcagcaaga cattcatccc
     5041 cggcgaactc cggtcagttt gaactcgcgc cgggttgtcg cgataccttc tcgcgccagc
     5101 tccatcaggt aaaccagggt tccgctctgt cgatcatagg cgaagaggcg ctggaacgaa
     5161 gcaccctcaa agagaagggc caacagtggc tgaccgtcaa tgtagacggg attttcaggc
     5221 actgacacgg tcacgccggt gatggcgtct ctatcgagca cttggcccgg cttgagcact
     5281 gccagcgcct caggaggcag gaaaatgccg ccaagcggca ctacggtcga cgtaaccgaa
     5341 tgcatctcac cgctcttcat cccgctcaga tactgttcta cctgcaccac ggcaagagtc
     5401 gattcaacgt gcacgatcct ggcaagcatc gttgtcggca tgggaatgac ggacgaaccg
     5461 tatcccacgt tcaccattgt cgatccttcg tatacaagct gagtaccgac ttgggcccat
     5521 gccggcactg acccgcctgc ccaggcaggt tccgtctgcc gcacggagat gaactccatc
     5581 accatgctgt tctgcctggc tgtatcgagg ctccccaagg tctgaattcg gtacacgagt
     5641 atgcctgtgg actcatcgaa aacgctaacc ctccgcgaca cgtcatccgc agcttcgaat
     5701 ctcactgctg tgatgggctt ccctgccatg gacgacggca tgtgcgtgat agccagatta
     5761 gcattggcct gcctctcggc agtgggcagc accgacggat gcagccatac gtcccccgct
     5821 cccgccaccc caacgacaaa ccctacgtcg cgcagtggcg ccagaccgcc gccggagatc
     5881 acgctgatcc ccagagcggc aatgactcgg ccggcatcgg tcgatacgac atcgtagttg
     5941 gccacgctct cgcccgcagg cgtcgtgtcg ccttcccttg tgtagtatga cgtcgcgcga
     6001 tctcgatgcc gggtttaccc ctttgtagtt gaatacagcc cctctctgct gatgaagtat
     6061 ttcgacatgg tgaagaacag gatgaacatg ggcaagctcg ccatcgcggc gatggccatc
     6121 atgatcccct cgtcgctgtg ggtctctgcc atgaatttgg taatgtacag cgggatcgtc
     6181 ttcatctctt cgctctgcac ggcaagcaag ggccagagta ggctgtccca ctgcgaacgg
     6241 aaggtcagca cagccagtgt ggcgatggcc ggcccggcat tgggaagcac tatgctggca
     6301 aatatcctag gttcgctggc gccgtctatt cgggccgcat ccagcacttc atcggggaat
     6361 gtcagcaaaa actgcctcat gaggaagatg ccaaaggcgc tagtcaggaa gggcacaatg
     6421 agccccgcat agctgtcctg caggcccatg cccatgacca ccatgtacag cgggatcatg
     6481 atggcctcga aggggatcat catcgtcgcc atgatggtca tgaacaccac gttgcgcccc
     6541 ttgaactcga atttcgccag gccgtatcct gtcatggacg atagcagcac tgtggacgct
     6601 gtcaccgtca cagcgacgat gagcgagttc agtgtgttcc gaaggagtat gaaatcgccc
     6661 tcaggccctc tcagcccatt ccagaagttc tgccagtgta tatgatcgct gaaccaggga
     6721 tacggcagct tcatgatgtc aatcgccggc atcgtagacg ccgtgaacat gaaggccagc
     6781 ggtatcagga ccagcgcaca tgctccgatc aaacacaaga gtataagaat gtcggctacc
     6841 ttcgacctac tcatgtcgtt cacacctcta tttcgcgctt atcgggcccg gctcttctgc
     6901 ccggtcatca atctagagcc gtcagtcgtc ggcctctgtc cggtcgggct gcccgcgcgg
     6961 gctacatgta ggtgacctcc tcggtccttg acagcctcag ttgaagccac gtaaacacga
     7021 gcatgaccag aaagagcagc acactcatgg cgctggccct cccaatgctg tggtccttga
     7081 tcgcagtgta gtagatgttc agggtgagga cattaatggg ctgtctgggg gccccggccg
     7141 ttgtaaatag gtactgcgta ctgaacgtct tgaggcactg gatcattgcc atgaccgata
     7201 ctagcagcgt agtgggtttc agaagcggga acgtgatgtg ccagaagtcc tgccatctgt
     7261 cggcgccgtc gatcagggct gcttcgtgca gagtatgggg gacagcgccg atcccggcta
     7321 cgaagatgac agtgaagtag ccgacgtact tccaaacata cacgatgatt gtagataagc
     7381 gcagcattgc atcgttggaa agccagtggt agttgacccc cgacgtacca aacaagagat
     7441 tgaggccctg gttggcaagg cccctggggt cgaagatgag cagccagatc atggccgcca
     7501 ccaccgatga gagcgccgcc ggcgaataca gcaccatctg gtagaacttc tggaacctct
     7561 tgcgcgacat tatgaaggca gccagtatga ggctgagcac cagcagcggc acgaaggtgc
     7621 cgaacgtgaa tatcgctgtg ttcaccacag actccttgaa caatagcgaa ctgaaaagat
     7681 atgtgtagtt gccgaagcct atgaatgttg gcggtttcag cgacaacagg ttcttccgga
     7741 agaagctgag atagaaggcg ttaaagattg gatagaagct gaacacggcg aagaagacta
     7801 aagacggcgt cacgaagatc catccccatc tggccttctt ctgctccatg ctgaaccgct
     7861 t
//
LOCUS       DAOTAC010000098         7726 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_8067_length_7726_cov_4.304524, whole genome shotgun sequence.
ACCESSION   DAOTAC010000098 DAOTAC010000000
VERSION     DAOTAC010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7726
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8067_length_7726_cov_4.304524"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="PLM74_05525"
     CDS             <1..409
                     /locus_tag="PLM74_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HON42580.1"
                     /translation="VDTSALFALLGSNDINHESAWGIWEGLLRDETPLVCSNYIVVEA
                     AALVQRRIGMEALRAFRECVCPMMDVEWVDRATHDSAETALLTANRRSLSLVDCTSFI
                     VMRRLGIARAFAFDVHFAEQDFMCLPEMRLHDR"
     gene            complement(411..1178)
                     /locus_tag="PLM74_05530"
     CDS             complement(411..1178)
                     /locus_tag="PLM74_05530"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HON42581.1"
                     /translation="MAGNARAALALMRVAFRRNRLSAAGFWIWLVSAGLYQLLGLAFV
                     WVASSRLSAGSEWLATYVYGVFQGATGLAYAFGAWTVFFASRYIRRGELDALLLLAPD
                     PRVSLPFANFEPLELVGALSGYAIALIGLRAGARLTVLNAAITVAGIACGGAATYSLF
                     MAAATLSLYTKQSSAAISFAMDLVLFGQYPMNLYPASMRTIFTWVIPIVLIADAPARL
                     VDAGPGLLAAVLAAAAIAFAGANVLFKQALGRFIRHW"
     gene            complement(1168..1962)
                     /locus_tag="PLM74_05535"
     CDS             complement(1168..1962)
                     /locus_tag="PLM74_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42582.1"
                     /translation="MNGFGARPQGLDKATGIRLLHIAAILAKERTTPVWDFVMRTLGS
                     TVLLIAQFAFLRGLDPSGDALRYLALARTAFACSGGQLLERFLIDGYMNGSLMMDVIT
                     PIYPLAFIMARSFAGMLASAAATLPGVLVAALLFGGMPRVSLQGAALYAASLIFVWLA
                     STGIALISSAIGAALKQGEAAVQLRVFVTQLASGALIPVYLYPDWVQRVIRVLPFVHL
                     ADTSVAIALGASVSSMYPQIIWSAALLGIGIALWRRRLARLDVYGG"
     gene            complement(1928..2644)
                     /locus_tag="PLM74_05540"
     CDS             complement(1928..2644)
                     /locus_tag="PLM74_05540"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HON42583.1"
                     /translation="MQHSVEFDHVCIEYESWRYERMRRVTQRIRALDDITLSINPGEI
                     VGVKGPNGSGKSSLLRAVCGVHTPVSGAVLVFGKPPNLFDPEFKRRITYSSSRRTSLL
                     PDLPLIDSFILRRELFAMPRAHWEGRMHSLVDRLSLGALMNRPPREFSQGQRARAELA
                     IALLHGPSLILLDETTEALDSEYMPRFLALLDELSTDGATVCMISHRPDDLECAHRIV
                     TIDGGRVVGDERVRGEATGT"
     gene            complement(2650..3165)
                     /locus_tag="PLM74_05545"
     CDS             complement(2650..3165)
                     /locus_tag="PLM74_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42584.1"
                     /translation="MRKVGTSRLLLLKAEIASDLEAIDKIGEAVSEAMLAVAPDQVPS
                     PVVQSGLALYLHDFYQAVEQILTRITRTLNGFDTAGESWHIDLLRLASREIEGLRPAV
                     ISESTMRDLDRYRGFRHFVRHGYDRSLMWDRMQGLIESLPEMVASFRQDTGAFLDVLT
                     QMINEIERRQC"
     gene            complement(3122..3511)
                     /locus_tag="PLM74_05550"
     CDS             complement(3122..3511)
                     /locus_tag="PLM74_05550"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HON42585.1"
                     /translation="MDVRSYVDGYIRRERRRLQACEERRTRALEVAKQAAALLADAGA
                     TKVVLFGSLGRGQGFHLHSDIDLAVRGISWAEYWRLWSAVDALTDIPVDLVMLDGASS
                     SLAERIGLEGVVLYEEGGHQPITPAEG"
     gene            complement(3722..4185)
                     /locus_tag="PLM74_05555"
                     /pseudo
     CDS             complement(3722..4185)
                     /locus_tag="PLM74_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567246.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylated DNA-protein cysteine
                     methyltransferase"
     gene            complement(4283..5257)
                     /locus_tag="PLM74_05560"
     CDS             complement(4283..5257)
                     /locus_tag="PLM74_05560"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HON42586.1"
                     /translation="MTVREVVCKSALTRTGIPGARYALNPYTGCAHACVYCYASFMTR
                     FSGHTDRWGTWVDAKVNIAEVLEKEIRRKSTRNRRSEKLIQPSLAGLGESAKPASRES
                     SRSPGLAESPEEPATPDSLDSAGRPTVLLSSVTDPYQPVEASLCLTRACLKALANAAN
                     PLAPEVSILTKSALVARDIPILRDLPGSEVGMTVTTLDDDLARQIEPGASAPSARLKA
                     LEQLASQSIKTWVFISPVLPHYGDSPAALAAVMKRAREAGVSRIGVDRLNLYPAAMAG
                     LARISRPEAVQALRAYRSSPQDYLDRLRSTVSEAAAAANIGIPVQVFF"
     gene            5459..6646
                     /locus_tag="PLM74_05565"
     CDS             5459..6646
                     /locus_tag="PLM74_05565"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HON42587.1"
                     /translation="MAEYAIETIGLGRRFGRIEASTDVSLTVPTGELVGLIGPNGAGK
                     TTLTMLLLGILRPSRGKALVLGADATYEFSRVGSAVGVVLDVHGLYEGLTAFQNLLFF
                     GELFSLSREAASQRASELLRVAGLWNRRHDRVAAYSKGMKQRLAIARSLMHHPRLLIM
                     DEPISGLDPESRRATKDLLNLPRLKSWEVQALVRDAAELREAPAVILDLRSNGGGSDW
                     YPLEWVRRFAGADVCPAGFDAQLVTSAAAKMLQNTASSLGAVLPMVDDSAGLADVQSW
                     LAGMADLDRPGWGRVSAAESVRAENDTLLVVLIDSYIYSAGESFVGFLRQVDDVVFVG
                     TNTYGAMLAGNVGVCLLPNSRLELSCGSLISLGEDLCNYDGRGHTPDFWVRPDEALER
                     AVK"
     gene            complement(6732..7124)
                     /locus_tag="PLM74_05570"
     CDS             complement(6732..7124)
                     /locus_tag="PLM74_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013013884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HON42588.1"
                     /translation="MSGEPWRAWIQRAESNLARARLGRQSEDILYEDICFDAQQAAEK
                     ALKGLLVYSGLDVPRTHSVGYLLSIIKESSGMPVPPQVEQAALLSDYAVTTRYPGDWE
                     PVGEDEYKQALAHAEEVWNWVVSVTGHK"
     gene            complement(7114..7419)
                     /locus_tag="PLM74_05575"
     CDS             complement(7114..7419)
                     /locus_tag="PLM74_05575"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HON42589.1"
                     /translation="MADVLQAIIDRIVETAEPDRIVLFGSRGQGKSTAASDYDILVLK
                     RGVTHRRRLAQAIYLALADIPAPVDVIVEDPDRIEKYRNTPGFIYSEALKGRVVYER"
     gene            complement(7504..>7726)
                     /locus_tag="PLM74_05580"
     CDS             complement(7504..>7726)
                     /locus_tag="PLM74_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018305168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42590.1"
                     /translation="YAESGGNADMEERISDELCKLCLETHNGFGKIADQCSDLTAYGV
                     QTRYPIGLTRSRIAPPCTRRYLEQKGFTA"
BASE COUNT         1504 a         2291 c         2356 g         1575 t
ORIGIN      
        1 cgtcgatact tcagcgctct tcgccctact cggttcgaat gacatcaatc atgaatcggc
       61 atggggcata tgggaagggc tactccggga cgaaacgccg cttgtatgca gcaactacat
      121 cgtggtcgag gccgcggccc ttgttcagag gcggatcggg atggaggcct tgcgggcgtt
      181 tcgtgaatgc gtctgtccga tgatggatgt cgaatgggtt gatcgtgcca cacacgattc
      241 ggcagagacc gctctcctga cggcaaatcg ccggagccta agtcttgtcg attgcacgag
      301 ctttatcgtg atgaggcggc tggggattgc tagggccttc gcgtttgatg ttcactttgc
      361 cgagcaggat tttatgtgtc tgcccgagat gcgccttcac gaccgatagc tcaccagtgc
      421 ctgatgaacc tgccgagtgc ctgcttgaac aggacatttg ctcccgcgaa ggcgatcgcc
      481 gctgcggcga gcacggcggc aagcaggcct gggccggcat cgaccaggcg ggccggggcg
      541 tccgcgatca gcacgatggg aatgacccat gtgaagatgg ttcgcatgga ggcagggtac
      601 aggttcatcg gatactggcc gaacaacacg agatccatgg cgaagcttat cgcagccgac
      661 gactgtttgg tatagagcga cagggtcgcg gccgccatga acaggctgta tgttgcggcg
      721 cctccgcagg cgatacccgc cacggtgatg gcggcattga gcacggtgag cctcgctccg
      781 gcgcgcaacc caatgagggc gatggcgtag cctgagagcg ctcccacaag ctccaagggc
      841 tcgaagttgg cgaagggcag cgacacgcgg ggatcgggcg ccagcaacag caaggcgtca
      901 agctcgcccc ggcggatgta gcgggaggca aagaagaccg tccaggcgcc gaaggcgtag
      961 gcaaggcctg ttgccccctg gaaaacgcca tagacgtatg tggccaacca ctcggagccc
     1021 gcgctgagcc tggacgaggc gacccaaaca aaggccaacc cgaggagctg gtatagtcct
     1081 gccgacacaa gccatatcca gaaccccgcc gccgacagtc tgtttcggcg gaatgccaca
     1141 cgcatcaggg ccagcgctgc cctggcatta cccgccatat acatccaggc gcgcgaggcg
     1201 cctcctccac agcgcgatcc cgattccgag cagggccgcg ctccagatga tctgaggata
     1261 catgctgctc acactcgctc ccagggcaat ggcgactgaa gtatcggcaa gatgaacaaa
     1321 gggaaggacg cgtatcaccc gctgaaccca atcgggatac agatagactg ggatcagggc
     1381 cccggacgcc agctgcgtaa cgaacaccct cagctgaacg gcggcttcgc cttgcttaag
     1441 agctgccccg atcgcggatg agatgagcgc gatccctgtc gacgccagcc atacgaagat
     1501 gagcgacgca gcatagagcg ctgcaccctg caacgacacc ctgggcatgc ctccgaacag
     1561 gagggcggcc acaagcacac ctgggagggt tgcggcagcc gacgcgagca taccggcgaa
     1621 tgaccgggcc atgatgaacg ccaatggata gatgggtgtg atgacgtcca tcatgagcga
     1681 gccgttcatg tagccgtcta tcaggaatcg ctcgagaagc tgccccccgg agcaggcgaa
     1741 cgccgtgcgc gcgagcgcca ggtagcggag ggcatcgcct gagggatcca ggcctcgcag
     1801 aaaggcgaac tgtgcaatca gcaggacagt tgatcccagg gtccgcatca cgaagtccca
     1861 caccggagtt gtgcgttcct tcgccagaat ggcggctatg tgcagcagcc tgattccggt
     1921 cgctttgtca agtccctgtg gcctcgcccc gaacccgttc atctcccacg accctccctc
     1981 cgtcgatggt cacgatgcga tgggcgcatt cgaggtcatc ggggcgatgg ctgatcatgc
     2041 acacagttgc cccgtccgtg ctcagctcgt ccagcagcgc caggaaccta ggcatgtact
     2101 ccgaatctag ggcctccgtg gtttcgtcca gcagaatgag gcttggcccg tgaagcagag
     2161 cgatggcgag ctccgcccgg gcacgctggc cctgggagaa ctcccgtggg ggccggttca
     2221 tgagggcgcc caggctgagg cggtcgacga ggctatgcat gcggccttcc cagtgcgcgc
     2281 ggggcatggc gaacagctcg cggcggagga tgaacgagtc gataagaggc aggtcgggga
     2341 ggagggacgt ccggcgtgag ctggaatacg tgattctccg cttgaactcg gggtcgaaaa
     2401 ggttgggcgg cttgccgaaa accagcacag ccccgctcac cggcgtatga accccgcaca
     2461 ccgctcgaag caggctggac ttgcccgatc cgttaggacc tttgactccg actatctcgc
     2521 ccgggttgat gctgagggtg atatcatcga gcgcgcgaat ccgctgcgtg accctgcgca
     2581 ttcgctcgta gcgccaggat tcgtattcga tacacacgtg gtcgaactca acggagtgtt
     2641 gcatatgact caacactgcc ttctctcgat ttcgttgatc atctgagtca gtacgtctag
     2701 aaatgcgccg gtatcctgtc tgaaggatgc aaccatctct ggaagactct ctatcagccc
     2761 ctgcatccga tcccacatga ggcttctgtc gtagccatgg cgcacgaagt gccggaatcc
     2821 gcggtagcgg tcgagatctc gcatggtaga ctcagagatt accgcaggcc gcagcccttc
     2881 gatttcacgc gatgccaacc gcagaaggtc gatgtgccag ctttcgcccg ctgtgtcgaa
     2941 tccattgagc gtcctggtga tgcgcgtcag tatctgttct acggcctggt agaagtcgtg
     3001 tagatacagg gccaagccac tttgcactac cggcgatggg acttggtcag gcgcgacagc
     3061 cagcatggct tctgaaacgg cctctccgat tttgtcaatg gcttcgaggt cgctcgcaat
     3121 ctcagccttc agcaggagta atcggctggt gcccaccttc ctcatacaga accaccccct
     3181 caaggcctat ccgttccgcc agagacgaac tggcgccgtc tagcatgacc aggtcgacag
     3241 ggatgtcagt gagggcgtcc accgcggacc agaggcgcca gtactccgcc caggagatgc
     3301 ctcggaccgc aagatcgatg tcgctatgga gatggaatcc ttgcccgcgt ccgagcgagc
     3361 cgaagagtac gacctttgtg gccccagcgt cagccagcag cgcagcggcc tgctttgcga
     3421 cctccagtgc ccgcgttcgc ctctcttcgc acgcctgaag ccgacgcctc tccctgcgga
     3481 tgtacccgtc gacatacgaa cgcacgtcca tctgatcctg cctcccagcc aaccttatcc
     3541 atatggtagc atatcctctg cgccgatgcc atgaaccgcg aatcgagccc tgcgcttgtg
     3601 ttcttgtgga cgttcgacag cctctacgaa gcgcatgcat tgcggagcac tttggagtcg
     3661 accatgcagc gctgctatga aggttgaggc agtgctaata tcttggggac cgtcttgtgg
     3721 cctaatcttc cagttcaaag aggctaccct cgtaatcctt cacgaagtat ctcttgcctt
     3781 tctggactat ggtatggcct tcagcctcaa gacgaagctt ttgaccgtct atgccctccg
     3841 gatacttctc attcagttcg ccgtcttttt tgagagttct ccagtaagga gtcgtgtcaa
     3901 catcgcgctc aaccgaagcc ttggctgcga tgtttatgaa tatgcctgct gtcaaagggc
     3961 acgtgttgtc ggctccatgc ttcttcgcca gatagctcct tatgtagtcg gatgtgatga
     4021 ccttgccact gggcactctt ttcatgactt cgtcataggc cagcggggag cgattagcag
     4081 tctcgttcca ccgtacattg agatcgccct tggatcggtg atttcggtaa tcttaggcat
     4141 gtccttgcta tcctgaagtt tctggttata ggacttcttc gccatacagg accaactcct
     4201 ttcttgttgg ccgcatactc cggattgctg cgctcaggcg gcaatccatc gcaagtggcc
     4261 ggcgccggct cgcccattcg cgtcagaaga acacctgaac cgggatccct atgttagctg
     4321 ccgccgcagc ctcagagact gtgctccgca gacgatccag gtagtcctgg ggtgaggacc
     4381 tgtatgcccg gagcgcttgg acggcttccg ggcgcgatat cctggccagt cccgccatcg
     4441 ccgctgggta caggttcagc cggtcgacgc cgattctgga caccccggct tctcgagcgc
     4501 gcttcatcac ggcggcgagc gcggcgggcg agtcgccgta atggggcaga actggggata
     4561 tgaagaccca ggtcttgatt gactggcttg ccagttgctc caaggccttc agcctggcgc
     4621 tgggcgcgga cgcgcctggt tcgatctgtc gcgctaggtc atcatcgagc gtcgtgacgg
     4681 tcatgcccac ttcgctgcct ggcagatctc gaaggatggg gatatccctg gctaccagcg
     4741 ccgatttggt cagaatagac acctcgggcg cgagggggtt cgcggcgttg gcgagcgcct
     4801 taaggcatgc gcgcgtcagg cagagagaag cttcgaccgg ctggtacggg tccgtgacgc
     4861 tggaaagcag cacagtgggg cgaccggcgg aatcgagcga atcgggcgtg gcgggttcct
     4921 cgggtgactc ggccagcccg ggcgatcggc tagactcgcg cgaagcgggc tttgccgatt
     4981 cgccgaggcc ggcgagggac ggttggatga gcttctctga tctgcgattg cgcgttgact
     5041 tgcgtcggat ttccttctcc agaacctcgg cgatgttcac ttttgcgtcg acccacgtgc
     5101 cccaacggtc ggtgtgtccg gagaatctag tcatgaaaga cgcatagcag tacacacagg
     5161 cgtgcgcgca cccggtgtac gggttgaggg cgtaacgggc tccgggaatc cctgtccggg
     5221 ttagggcgga cttgcacaca acctcgcgga ccgtcacccc attgggatct cacgtccttt
     5281 cgcgaatacc agagttctct gcgcctggcc gcattccttt cgcatgaggc taatcatcgc
     5341 accaagatga agaacatccg aagatcacta ggatggcgtg ttgtggatac accccatata
     5401 tgggagaatt gagccagccg gagagccccc catttcgcag acgggagatg gtttgaccat
     5461 ggccgaatac gccatcgaga cgattggcct tggaaggcgg ttcggacgca tcgaggcttc
     5521 gaccgacgtg tcgttgactg tgcccacagg cgagctggtt gggctgatcg gacccaacgg
     5581 cgccggcaag accacgctca ccatgctgct tctggggata ctgaggccgt caagagggaa
     5641 ggcgcttgta ctcggggcag atgcgaccta cgagttttcc agagtcggaa gcgccgtggg
     5701 tgtggtgctg gatgtacatg gcctgtatga gggccttaca gcattccaaa acctactctt
     5761 ctttggcgag ctgtttagct tgtcacgcga ggctgcgagc cagagggcca gtgaactgct
     5821 cagggtggca ggtctctgga atcgtcgaca cgaccgagtg gccgcgtatt ccaaggggat
     5881 gaaacagcgt cttgccatcg ctagatcttt gatgcaccac cccagactgc tcataatgga
     5941 cgaacccatt tccggtcttg accccgagtc acgcagggcc accaaggatc tcctgaacct
     6001 cccacgactg aagtcgtggg aggttcaagc attagtccgt gacgcagccg agctcaggga
     6061 agcgccggct gtcatcctcg acctgaggtc aaacgggggt gggagcgact ggtaccccct
     6121 ggagtgggtg aggcggttcg ccggcgctga tgtgtgtcca gccggctttg acgctcagct
     6181 ggtgacgagc gcagctgcga agatgctcca gaacacggcg tcttccttag gggcagtgct
     6241 gcccatggtc gacgattcgg ctgggttggc agatgtacaa tcgtggctcg ccggcatggc
     6301 tgatctggat aggcccggct ggggccgggt atctgctgcc gagagcgtca gggcagagaa
     6361 cgatacactc ctcgtcgtgc tgatcgactc atacatctac tctgccggcg agagcttcgt
     6421 cggattcctg cgccaagtcg atgacgtggt gttcgtgggc acgaacactt acggagcgat
     6481 gctcgcgggc aacgtcggcg tgtgcctgct tcccaactcc aggctcgaat tgtcatgcgg
     6541 cagtctgata tcgcttggcg aagacctgtg caactacgac ggtcgtggcc acacacccga
     6601 cttctgggtt cggcctgacg aggccctgga gagagcagtc aagtagattc atactgtgct
     6661 ggcaggcggg cgggagcgct aatgactgat gggttgcggg caagaaaccc ggcagagagc
     6721 cgtctttcga actacttatg gccggtgact gagaccaccc agttccacac ctcttcagca
     6781 tgtgccaggg cttgcttata ttcgtcttcg cccacgggtt cccagtcgcc cggataccgg
     6841 gtggtcacgg cgtagtccga caggagcgcg gcctgttcca cttgaggcgg cacgggcatc
     6901 ccgctgcttt ccttgatgat cgacaagagg tagccaacgg aatgggttct aggcacatcc
     6961 aggcctgagt acaccaagag gcccttcagg gccttctcgg cagcctgttg cgcgtcgaag
     7021 cagatatctt cgtacaggat gtcctccgac tggcggccaa gcctggcgcg cgcgaggttg
     7081 ctttctgctc tctggatcca tgcccgccat ggctcaccgc tcatagacca cgcgtccttt
     7141 gagggcttca ctgtagatga agccgggagt atttcggtat ttctcgattc tgtcgggatc
     7201 ttccacaatc acgtcaactg gggccgggat atcggcgagt gcaaggtaga tcgcctgcgc
     7261 caggcgcctg cggtgggtta cgccccgctt taggaccagg atatcgtagt cgcttgctgc
     7321 agtgctcttg ccctggcctc ttgacccgaa caggactatg cgatctggct cggcagtctc
     7381 gactatcctg tctatgattg cctgaagcac atcagccata cggctcacct ccgggttgga
     7441 cgccgctcca ctctctagcc tgatgctacc atcccgagat atcggcgaca atactccgac
     7501 ggctcaagcc gtgaacccct tttgctccaa ataccttcgt gtacagggcg gggcaatgcg
     7561 tgatcgggtc agcccgatcg ggtatcgcgt ttgaacgccg taggccgtca ggtctgagca
     7621 ctggtcggct atcttgccaa acccgttgtg tgtctccaag cagagtttgc acagctcatc
     7681 gcttattcgc tcttccatat cagcattccc accgctttca gcatag
//
LOCUS       DAOTAC010000099         7647 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_8174_length_7647_cov_4.902529, whole genome shotgun sequence.
ACCESSION   DAOTAC010000099 DAOTAC010000000
VERSION     DAOTAC010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7647
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8174_length_7647_cov_4.902529"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="PLM74_05585"
     CDS             complement(<1..603)
                     /locus_tag="PLM74_05585"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HON42591.1"
                     /translation="MRPRIIVLVSQRTCALMAAVVAVGVILYLLATYTVTVIAPARDI
                     VSGRTVAIDAGHGGPDSGARGKSGIREKHINLHIALELRALLGRAAVYTVMTREADHD
                     LIDDGEDITGSRKRAELERRATLVNASSPDIFVTIHSNSFPEPQWSGAQVFYNPVSDE
                     GRALAVRIQAQLVQRLGPNTRKARPADIYLLRRVKAPSALV"
     gene            complement(704..1363)
                     /locus_tag="PLM74_05590"
     CDS             complement(704..1363)
                     /locus_tag="PLM74_05590"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HON42592.1"
                     /translation="MWNYSGESRTSTTVNLEVCALAEQYFTPSPTSARQPQAVRYRMR
                     DGRELVFQTDAGVFSKGRVDRGTLELIKAIQDEDGQSFLDLGCGWGPVGIVAALSQPR
                     REVWMVDINERACELARANALANGAGRAIVVCGDGLAAVRGRKFDVIATNPPIRAGKS
                     VVYRLVEEARDHLNEGGRFYAVARTKQGAGSLRVFICEAFGNADEPEIGGGYRVIRAT
                     R"
     gene            complement(1420..2901)
                     /gene="ypeB"
                     /locus_tag="PLM74_05595"
     CDS             complement(1420..2901)
                     /gene="ypeB"
                     /locus_tag="PLM74_05595"
                     /inference="COORDINATES: protein motif:HMM:TIGR02889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="germination protein YpeB"
                     /protein_id="HON42593.1"
                     /translation="MADEISNVGDDMGAVAEGGDQGLGTTGRDTARPGGTGANWPAGL
                     LLVLLIASLVFGYAQWSTRRRLEVAVGNQYFNSFFDLLGNVDNVRVALSKSLVSGSPR
                     LRVGALSDVWREASNAQANLNSLPVTHKVLARTSAFLTQVSDFAFVTARKVANQQMMS
                     DDDWDRLVKMKEQSEDLAKGLAGMEAEASAGRINWVRVAEQAKKEVPAKSGSKSTPET
                     VTDGFTKVDESVQHFPTLIYDGPFSDHIEKREPVGVTGSPIDATQAIEIARKFVPFDA
                     SAHTGKIQGEIDSKMPAYRVSLEAPKKSGKPDAVVDVTKVGGHVVLMNISRDIDPKKI
                     DLEAAVTRASDFLSKVGMTSMKPTFASEVGDVAVIPFVFVQNGAYVYPDMVKVKVALD
                     NGDILSYDALGFLTSHHTRKLPKPSIEADEAKELLSPALTVSAPPRLAVIPVETVATP
                     EAFCWEFRGTSFGEDFFVYINAVSGEEEKVLQLIKTSEGSLTM"
     gene            complement(2920..3378)
                     /locus_tag="PLM74_05600"
     CDS             complement(2920..3378)
                     /locus_tag="PLM74_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HON42594.1"
                     /translation="MSWAMKRKLIAVSTVLIVTAVIGVALVWRSDAQGRAGDTNLLAR
                     LVGAEAEGEPYQGMVAVAAVMLNRVKDSKFPNTMSGVIFQPGAFESVSNGLIWQRSPS
                     RQAVSAARDALNGYDPTYGCVFFWNPSKPVSGWIWSRSIAVRIGQHVFAR"
     gene            complement(3405..4112)
                     /gene="sleB"
                     /locus_tag="PLM74_05605"
     CDS             complement(3405..4112)
                     /gene="sleB"
                     /locus_tag="PLM74_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic enzyme"
                     /protein_id="HON42595.1"
                     /translation="MNLHKRILGLAFSFTLAVVVVLLAPGLASAETRPTLYWGTRGRS
                     VRLVQWKLSEWGYYRGYVDGIYGARTYRAVVAFQRRNGLHVDGLVGGETWRALGFGYT
                     VATAARAPAAYATPSRSATVHLLARAVAAESHGEPYRGQVAVAAVMMNRLKNPRFPRT
                     LSGVIFQPRALESVSNGLYWRRTPDATAYAAARDAVNGFDPTYGSIFFWNPYKPVSGW
                     IWTRNIVTQIGKHVFAR"
     gene            4410..5408
                     /locus_tag="PLM74_05610"
     CDS             4410..5408
                     /locus_tag="PLM74_05610"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HON42596.1"
                     /translation="MISSVGITFNLKKDFPTRGGEDSHPPDLAAECEEEETVDMLASS
                     LARLAQKVLLFPHNPDMLDKLDQSRPDLVFNIAEGWFGRNRESLIPALLELLHIPFTG
                     SDALSLGIALDKAMAKRVVSTAGIRTPRWRKVSSAREAAQVDLAFPVFVKPNFEGSSK
                     GIRNSSRVNDHKELAEKVEWIAQTYGQPALVEEFLPGREFTVGIIGNGRPHVLPVMEV
                     VPGDRDSADHDFVYSYETKSGNLERFRVPAPISPELAAEISEMAVGVFETIECRDVAR
                     VDFRLDSEGRPNFLEINPLPGMSKVSLLPLQAKAAGMDFDDLVDWIVRSAWTRYYH"
     gene            5629..>7647
                     /locus_tag="PLM74_05615"
     CDS             5629..>7647
                     /locus_tag="PLM74_05615"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HON42597.1"
                     /translation="MKRTRHAALLALIACLLAVIVSTQAGAQAALAGEKTYIELILDS
                     SISMSAFVEGWKSRMDVAKAVMEQLIRDLPDDPNLMVALRIYGAELRPNLTPCEDTVL
                     VQPFAPVAKARQNMIDQVKSMKARGMTPIALSLQQAAKDFPDKNARNIIVLITDGEES
                     CGGDPCAVSARLQADGFVMKPYVVGFALKPQEVEKVRCIGEYYTASDTASLRKALATI
                     MARAVAPAQIEVQAWAGAMNVTGQAEIEIVKSTGEIVQSTRPALDPSVVRAQVDEGQY
                     IVRGRLLVGSQILTAQAASITAKPGQTSVVRLDFGSLDGRVRVTARASGQDVSDRVDI
                     RVLRAGKPVASSWAGIPPTATLPAGDYTVVVTHRQYPELTKTATATVQTGRDTYVDVD
                     LGELPATLEVTVTYRGAIMSNLCQLSVLAPGQATQVIRNTADGRVFRWTSTPGMYDLN
                     VMYRDVVTAEKAVRGVQLAGGRTTSITVNLDDLLGALRVRVVAGGRDVTADSKVMAAG
                     RDGKTELPFAGGMRQALVISGVYAVSAIYRDTESDMHEAYVKPGDTTDLTIEVKLPGR
                     IAIIPTIEGKPMRPDRLRAWAYLDGASVGNTVVQSDRVEIVVPEGKYNVVGEISEPFA
                     QRRESPGVQVRAGETVQVKMNFDPAGLLRIKVIADGKPILARAWVHFFG"
BASE COUNT         1571 a         2374 c         2193 g         1509 t
ORIGIN      
        1 cacaagagca gacggcgcct tcacccttcg aagtaggtat atgtcggccg gacgcgcctt
       61 cctggtattc gggccaaggc gctgcacaag ttgggcctgg atgcgcacgg ccaacgctct
      121 gccctcgtcg gagaccggat tgtagaagac ctgagccccc gaccactggg gctcggggaa
      181 gctattggag tgtatcgtca cgaaaatatc gggcgaactg gcgtttacca gcgttgcgcg
      241 gcgctcaagc tcggctcgct tgcgcgaacc ggtgatgtct tctccgtcgt caatcagatc
      301 atggtcggcc tctcgcgtca tgaccgtgta taccgcagcg cggcccagaa gcgcgcgaag
      361 ctccagcgcg atatggaggt taatgtgctt ctcgcggatc ccgcttttcc cccgagcccc
      421 ggagtcgggt ccgccatgcc cggcatcaat ggccacagtc cgccctgata cgatgtctct
      481 tgcaggggca atgacggtca ccgtgtaggt cgcgagcaaa tacagaatga cgcctacagc
      541 aaccacggcg gccataagcg cgcaggttct ctgggagacg agtacgataa tacgcggtct
      601 cacggagcag acctccagag gccaaggctc gcgccgctcg cggcctcagc catttggagg
      661 atatgccccg gaccagcccg cgatgaccgt catgctcaaa cgcctatcgc gtcgcccgga
      721 ttacccgata ccctcctcct atctccggtt cgtcggcatt tccgaatgcc tcacagatga
      781 agacccgcag cgagcctgcg ccctgcttcg tccttgccac tgcgtagaag cgtccgccct
      841 cgtttaggtg gtctcgagct tcttcaacca gcctgtagac gacggacttg cccgcccgga
      901 tcggcgggtt cgtggcgatg acgtcgaact tcctgcccct gactgctgca agaccatctc
      961 cacatactac tatcgcgcgg ccggcgccgt tggccagggc attggcgcga gccagttcgc
     1021 aggcacgctc gtttatgtca accatccata cctcacggcg cggctgtgac agagccgcca
     1081 cgattcccac tggtccccag ccgcatccca ggtccagaaa ggactgtccg tcctcgtcct
     1141 gaatcgcctt gatcagctca agagtgccgc gatccacgcg gcctttggag aatacgcctg
     1201 cgtcagtctg gaaaaccagt tcgcgtccgt cacgcattcg ataacgcact gcctggggct
     1261 gacgggcgga tgtgggagag ggtgtgaaat actgctcggc cagcgcgcac acctccaagt
     1321 tgactgtagt gcttgtgcgt gattctcccg agtagttcca cacccctgcc ggcccatcct
     1381 ccactctccc acaaggcgct ccccgcaaga cgccgggctc tacatcgtca ggctgccctc
     1441 ggatgtcttg atgagctgca gaaccttctc ctcctcaccg cttacggcat tgatgtacac
     1501 gaagaagtcc tcaccgaagg aagtcccgcg aaactcccag cagaaggcct cgggagtagc
     1561 cactgtctcg acgggaatga ccgcgaggcg cggcggcgcg ctaactgtga gcgcagggct
     1621 taggagttcc tttgcctcat ccgcctctat cgaaggcttc ggcagcttgc gcgtatgatg
     1681 cgacgtgagg aaccccagcg cgtcatagga gagtatgtcc ccgttgtcca gcgcgacttt
     1741 gaccttcacc atatcggggt aaacatacgc tccgttctga acgaacacga agggaatgac
     1801 cgcgacatcg ccgacctccg acgcgaacgt cggcttcata ctggtcatgc cgaccttcga
     1861 caggaagtcg ctggctcggg tcaccgccgc ctccagatcg atcttcttcg gatcgatgtc
     1921 tctcgatatg ttcatgagca ctacatgccc gccaacctta gtcacatcaa ccaccgcgtc
     1981 aggtttgccg cttttcttcg gcgcttcgag acttacgcga taagcaggca tcttgctatc
     2041 tatctcgccc tgtattttgc ctgtatgggc cgaggcatca aatggaacaa acttgcgggc
     2101 tatctcgatc gcctgcgtgg catcgatcgg ggatcccgtg accccaacag gctccctctt
     2161 ctcgatatgg tccgagaaag gaccatcata tatcagcgta gggaagtgct gcacagactc
     2221 gtcgactttg gtgaaaccgt cggtcactgt ttcaggcgtc gacttggatc cggatttagc
     2281 cggcacttcc ttcttcgcct gctccgccac acggacccag ttgatccgac cggccgaagc
     2341 ctcagcctcc atgcccgcaa gtcccttggc cagatcttcc gactgctcct tcatcttgac
     2401 gagcctatcc cagtcatcgt cgctcatcat ctgctgattg gccaccttgc gcgctgtcac
     2461 gaaagcgaaa tcgctcactt gcgtaaggaa ggccgatgtc ctggccaaca ccttgtgcgt
     2521 caccggcagg ctattcaggt tggcctgagc gttgctggcc tcgcgccata catccgacaa
     2581 agcgcctact ctaaggcgcg gcgaacccga taccaggctc ttggaaagcg ccacccgcac
     2641 attgtccaca ttgcccagaa ggtcaaaaaa ggagttgaaa tactggtttc ctacagcgac
     2701 ctccagtcgc ctcctggtgc tccactgcgc gtacccaaag accaaacttg caatgagcaa
     2761 tacgagcaga aggcccgccg gccagttggc tccggttccc cccggtctgg ctgtgtcgcg
     2821 tccggtcgtg cccaggccct gatctccgcc ctccgctaca gcgcccatgt cgtcccctac
     2881 gttactgatc tcgtctgcca tgtacgacag ataccccctt taccgagcga atacgtgctg
     2941 gcctatgcgc acggctatgc tcctgctcca tatccacccc gataccggct tggaagggtt
     3001 ccagaagaac acgcacccat acgtgggatc atatccattg agcgcatctc gggcagctga
     3061 cactgcctgg cggcttgggc ttctctgcca tatcaacccg ttggatacag actcgaatgc
     3121 gcccggttgg aagatcactc ccgacatggt atttggaaac ttcgagtctt tgaccctgtt
     3181 gagcattacc gcggcgacag ccaccatccc ctggtatggc tctccttcgg cttcagcacc
     3241 caccagacga gcgagaaggt tagtgtcgcc tgcccgccct tgcgcatcac tgcgccatac
     3301 cagggcgact ccgatcacag cagtcacgat cagcaccgtt gaaactgcta tcaatttccg
     3361 cttcattgcc cagctcatca agtctcctcc tagcgggaaa ccgtctatcg cgcgaacaca
     3421 tgctttccga tctgcgtcac tatattcctg gtccaaatcc accccgacac gggcttgtac
     3481 gggttccaga agaagatcga tccataagtg ggatcgaatc cgttcaccgc gtctcgggcc
     3541 gcggcgtacg cagtcgcgtc cggggttctg cgccagtaaa gcccgttgga gaccgactca
     3601 agcgcgcgcg gctggaatat cacgccagag agcgtgcgcg ggaaccgcgg gttcttcaac
     3661 ctgttcatca tgactgccgc tacggccacc tgaccgcggt agggctcacc gtgcgattcg
     3721 gcagcaaccg cgcgagctaa aagatgcact gtggcagatc tggacggcgt cgcgtacgcc
     3781 gccggcgccc gggctgcggt cgccaccgtg tagccaaatc ccaatgcccg ccaggtctcc
     3841 ccgcccacaa ggccgtcgac atgcaaaccg tttcgtcgtt gaaaagctac tactgctctg
     3901 tacgtccgag cgccgtatat cccgtccaca tagccacggt agtagcccca ttcagaaagc
     3961 ttccattgga cgagcctgac agatcgtccc cttgtgcccc agtacagcgt gggcctcgtc
     4021 tcagccgacg ccagcccagg cgctaggagc accaccacta ctgcgagggt gaacgagaat
     4081 gccaaaccca gaatccgctt atgcaggttc atgatacaga tgcacccctg tctggttgga
     4141 tgacgcactg tgctgccact attctccctt ggatttgcgc cgcgtattcg ccttgggaca
     4201 ggcagcgcaa caccttacag gaacccgcgt gaagccgtta tagcgcgaga atcgaagaca
     4261 tggggagctc tagccctacc gttcgccatt cgcgcttcaa ggcgctcagt gcgggttctt
     4321 ccggacatcg actggggcag cctatggagc tattatcagc ttaaatcggg cgccaagccc
     4381 caaaatcgca cggggagctg tttgcgaaga tgattagctc cgtagggatt actttcaacc
     4441 tgaagaagga cttccccacc cgaggtgggg aagacagcca ccctccagac ctggcggcag
     4501 agtgcgaaga ggaagagacc gtcgacatgc tggcatcctc cctagcacgg ctcgcacaga
     4561 aggtcttgct gttcccccat aaccccgaca tgctggataa gttggaccag tcccgccccg
     4621 atctggtatt caacatcgcc gaaggctggt tcgggcgtaa ccgagagtct ctcatacctg
     4681 ccctcctgga gcttctgcac atcccgttta ccggatcaga tgctctgtct ttgggtatcg
     4741 ccctcgacaa ggctatggcc aagagagtgg tatccaccgc gggcatcaga accccccgct
     4801 ggcgcaaggt cagctctgcc agggaagcag cgcaggtaga tctcgcgttt ccggtatttg
     4861 tcaagcccaa cttcgagggt tcaagcaagg ggatccgcaa ctcctccagg gtcaacgacc
     4921 ataaggaact cgcagaaaag gttgagtgga tagcccagac ctatggccag ccggcgctgg
     4981 tcgaggagtt ccttccaggt cgggagttca ctgtcggaat catcggaaac gggcgaccgc
     5041 atgtcctgcc ggtgatggaa gtagtcccgg gcgaccgaga tagcgctgat cacgactttg
     5101 tgtactcata tgagactaag agcggcaatc tcgaacgatt ccgagtgccg gcgcccattt
     5161 ctcctgagct tgcggccgag atctccgaaa tggcggtggg ggtgttcgag accatagaat
     5221 gccgagatgt ggcaagagtc gattttcggc tggactccga aggacgcccc aacttcctgg
     5281 agatcaaccc gctccccggc atgtccaagg tgagcctcct gcctctgcag gcaaaggctg
     5341 caggaatgga cttcgatgac ctagtggact ggattgtcag gtctgcgtgg acgaggtact
     5401 accactagtc acacgcaggc gcgatttggt agcttgcgac ccgtgccact gccgctaacg
     5461 gctcttctga gatggccata gcggacgccg ggtcgtttgt ttttctcggc ccattcctgc
     5521 atgcgcagga atcttcgcat agctgtcaaa taatcaaggt gcatttcatg ccacagttgc
     5581 catgggcttc aggcgagaaa tagccaactc ctaaggaggg actgcacctt gaagcgcacg
     5641 cgtcatgccg cgttacttgc actgatcgca tgcttgctgg cggtcatagt ctcaacccaa
     5701 gcgggcgcgc aggcagcact tgccggcgag aagacctata ttgagctgat tttggactcg
     5761 tccatcagca tgagcgcctt tgtcgaaggc tggaaatcca gaatggacgt ggccaaggca
     5821 gtcatggagc aactcatccg cgaccttccg gacgacccca atctcatggt ggcgctcagg
     5881 atctacggag ctgagctcag gccgaacctt acgccgtgcg aagatacggt gttagttcag
     5941 cccttcgccc ccgtagccaa ggcacgacag aacatgatcg accaagtcaa gtcgatgaaa
     6001 gccaggggca tgacccccat cgccctctcc ctgcaacagg cggcgaagga cttccccgac
     6061 aagaacgccc gcaacataat cgttctgatc accgatggcg aggaaagctg cggcggcgac
     6121 ccgtgtgcgg tgtccgccag gctgcaagcg gatggattcg tcatgaaacc atatgtggta
     6181 gggtttgccc tgaaaccgca ggaagtcgag aaggttcgct gtatcggcga gtactacact
     6241 gcctccgata cagcatcact caggaaagct ctcgcaacca tcatggcccg cgctgtggcc
     6301 cctgctcaga tagaagtgca ggcatgggcg ggcgcgatga atgtgacagg tcaagccgaa
     6361 attgagatcg tgaagtccac aggcgagatc gtgcagtcga cccgccccgc gctggacccg
     6421 tcggtcgtgc gcgcgcaggt ggacgaaggc cagtacatag tgcgcggccg actcctcgtt
     6481 ggctcgcaga tactgacggc tcaggcggca agcatcactg ccaaacccgg ccagaccagc
     6541 gtagtgaggt tggacttcgg gtcgctggac ggcagggtgc gcgtgaccgc ccgcgcgtcc
     6601 gggcaggatg tgagtgaccg cgtggatatc cgggtgctca gggcgggaaa gccagtggct
     6661 tctagctggg ccgggattcc gcccaccgcg acgctgccgg ctggcgacta cacagtcgtc
     6721 gtcacccata ggcagtatcc tgagctcacg aaaacagcaa cggccacggt gcagacgggt
     6781 cgggatacgt acgtcgacgt cgacttgggc gaattgccgg cgacgcttga ggtgaccgtg
     6841 acataccggg gcgcgatcat gtcgaacctg tgtcaactga gcgtgctcgc tccgggacag
     6901 gcgacgcagg tgatacgcaa cacagcggat ggacgcgtgt tccgttggac ttcgacgccg
     6961 gggatgtatg acctcaatgt gatgtaccga gatgttgtca cagccgagaa agcggttcgc
     7021 ggcgtgcagc tggcgggcgg ccgaacaacc agcatcacag tgaacctgga tgatctcctc
     7081 ggcgctctca gagtgcgcgt ggtagccggc gggcgcgatg tgaccgctga ctcgaaggtg
     7141 atggcggcag gacgcgacgg caagaccgag ttgccgtttg cgggcggtat gcgccaggcg
     7201 ctcgtgattt ctggtgtata cgccgtaagc gccatctacc gcgacaccga aagcgacatg
     7261 catgaagcat acgtcaagcc gggcgatacg acggatctga ccatagaggt gaagttgccc
     7321 gggcggatcg cgataatccc cacgatcgaa ggcaagccca tgaggcccga tagactccgg
     7381 gcgtgggcgt atcttgacgg tgcttcagtc ggcaacactg tggtgcagtc ggatagagtc
     7441 gagatagtcg tgcccgaggg caagtacaac gtggtcggcg agatatccga accattcgct
     7501 cagcgcaggg agtcacctgg ggtccaggta cgggccggcg aaacggttca ggtcaagatg
     7561 aacttcgacc cggcagggct gctgcggatc aaggtcatag cagatggaaa gccaattctg
     7621 gccagagcgt gggtgcactt cttcggc
//
LOCUS       DAOTAC010000100         7598 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_8233_length_7598_cov_5.254806, whole genome shotgun sequence.
ACCESSION   DAOTAC010000100 DAOTAC010000000
VERSION     DAOTAC010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7598
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8233_length_7598_cov_5.254806"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1321)
                     /locus_tag="PLM74_05620"
     CDS             complement(<1..1321)
                     /locus_tag="PLM74_05620"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HON42598.1"
                     /translation="MNYDETMEYLGALARFGVKPGLERTDALLNFAGQPQQRMKHVHI
                     AGTNGKGSVAVMIESVLRAAGFRTGLYTSPHLQRYTERIRVCGQEIPGRELAALITSL
                     APAVQRLAGDPSIGAPTEFEVATAACFQWFASQEVEIAVVEVGLGGRYDSTNVITPEV
                     SVITHIAMDHMDKLGNTLGEIASDKAGIIKPGVSVVVGPQEPEVTRVLEDAARRAGAP
                     LTLLGRDVGFDVVSIDMNGTTIDMDLPSFGPVRATTGLVGRHQASNCATAAAALGVLA
                     GARKTSACVFVDDLRRGLAQAANPGRFEVVASAPFVVILDGAHNPDGARALAATVERV
                     LPGTGPRVLVVASSRDKRVDEMMQALAPCFDVVVATAAAHTRSGAADPEALAAAARAV
                     WEAGALRRRAGSGLGPGTIAEPGPEAYVKTPAHDAVQLGLAIARQRQA"
     gene            complement(1318..2088)
                     /gene="nth"
                     /locus_tag="PLM74_05625"
     CDS             complement(1318..2088)
                     /gene="nth"
                     /locus_tag="PLM74_05625"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011433732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HON42599.1"
                     /translation="MDTNHEMHAAADATSIAAACAERPRRVDGILAKMYGPKLLRPHG
                     DPVGELVHIILTQNTSDANSDRTYAALRAAYPLWEQLALAPPTRVADVIRMGGLADIK
                     AARIGEALRRIEADFGSISLAALRDMGDTDAFDYLTSLPGVGPKTAACVLLFALGRPV
                     FPVDTHVHRVSNRLGLVSTRSPAATQSLLMPAVPDDIVYQLHMNMVTHGRKTCKAGRP
                     ACSRCLLQSECDWACHAAQAVADGETGYAPSSGAGDDG"
     gene            complement(2089..4749)
                     /locus_tag="PLM74_05630"
     CDS             complement(2089..4749)
                     /locus_tag="PLM74_05630"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HON42600.1"
                     /translation="MGKTDIPTTYDPKQIEERIYQFWLDRGYFHAQPDPDPDREHFVI
                     MMPPPNITGTLHIGHALDSAIQDILIRSKRMQGANACWIPGTDHASIATEAKVVASLA
                     KQGLTKTDVGRDGFLKIAWDWKEKHGGEIVEQQKKLGASCDWERARFTMDPVCSRAVR
                     EVFVQLFEKGLIYRGERMVNWCTNCGTSLSDVEVEHEDEESALWYLRYPLTDGTGHVT
                     VATTRPETMLGDIGVAVNPSDPRYASLVGKTLMLPIMNREIPIVADEHVDPAFGTGAV
                     KVTPAHDPNDFEIAHRHGLESVCVIGPDGRMTGEAREYAGQTREECRRAVVAWFESAG
                     LLERVEKYTHAVGHCQRCGTLVEPLVSLQWFVRMEPLAKPAIEAVKNGSIRFVPERFT
                     KMYLNWMENVRDWCISRQLWWGHRIPVWYCDDCGHMFASREDAIACPKCGGRVEQDPD
                     VLDTWFSSALWTFSTMGWPDHTPELAAWHPTSVLVTGYDIIFFWVARMIFMALEFMGE
                     KPFSDVLLHGLIRNADGSKMSRSKGTGVNPLEIIEEYGSDTLRFTLITGNTPGNDFRW
                     RPEKVEASRNFCNKIWNASRFVLMNLGDFEAPAGGQLPPDLELTLADRWILSRFNRVA
                     GQITQQLARYDLGEAARTIYDFIWGEYCDWYIEIAKRRLYDDADKQGRRAAQYVLWKV
                     LDGALKLLHPFMPFITEGIWQHLPHEGDSIMIQAWPRMDEAWLDPEAEAEMAMIMETV
                     RAIRNVRAEFEVAPSRKVDAIFHADGQVAAALDRSADVLAHLAGLAAVKMESAAAPKP
                     EKAAAAVAPGVEVYLPLAGMIDIDKEIERLRRELENLQAETEKVQAKLGNESFVLKAP
                     PEVVQKQRDRLADLEDQLTKVRVRVAQLES"
     gene            complement(5226..7319)
                     /gene="fusA"
                     /locus_tag="PLM74_05635"
     CDS             complement(5226..7319)
                     /gene="fusA"
                     /locus_tag="PLM74_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HON42601.1"
                     /translation="MKKYRVDQLRNVAVISHGGAGKTTLAEAMLFLGGAVDRFGKVDD
                     GTSTMDYDPDEVKRKISINASVAPVEWNGHKINLIDTPGYSDFVGEVVGALRIADAAM
                     VLVDAVSGVEVGTELTWRHADDRKLPRIIVINRMDRENANFGKALDSIREIFGTNAVA
                     AQLPIGEGDKFRGVADIIHMKAFIWSDASGKNVAESPIPDELADAAGELREKLIEYAA
                     ESDEELLNKYLEGEELTQEEFERGLAAGVADGSVVPVFCASGLRMVGVKPLMDSIVAL
                     LPSPVAQGAIMGRLPGSEDSVERRPDEAEPFSALVFKTMADPYVGKITMFRIYSGVVK
                     SDSQVYNVSKDRIERFGQVFLIKGKQQIAVAEVGAGDIAGVAKLSETTTGDTFSDKDK
                     PVVLDPIKFPSPVLSMAARAKSKGDEDKIGQGLARLQEEDPTLRYTKEAETGELLLAG
                     MGEVHLDIIAERLKRKFGAEMTLDTPKVPYRETIKGTVKVEGRHKKQTGGRGQFGHVW
                     IEMSPLPTGSGFEFEDKVFGGAVPRQFIPAVEKGLREGLPEGGALAGFPLVDLKCSLY
                     DGSSHPVDSSEMAFKIAAQLALKKGCQDANPIILEPIVRAEVTVPEEYMGDVMGDFNK
                     KRGRILGMEPEGRYQVVRALVPMAEMFKYSIDLRSMTAGRGTFTTEFDHYEEVPFSVA
                     EKVIEQAKKDKEKES"
BASE COUNT         1416 a         2498 c         2117 g         1567 t
ORIGIN      
        1 ccgcctgcct ttggcgggcg atcgccagcc ccagctggac cgcatcgtgt gccggcgtct
       61 tcacgtaggc ctccggcccc ggctcagcga tcgttcccgg ccccagcccc gaccccgccc
      121 gtcgcctcag cgccccggct tcccataccg cgcgcgcagc agccgccagg gcttcaggat
      181 cggcagcccc cgacctggta tgcgcggcgg ctgtggccac caccacgtcg aaacaaggag
      241 caagcgcctg catcatctca tctacgcgct tgtcgcgaga cgaagctaca acaagaaccc
      301 tgggtcccgt gcccgggaga acgcgttcaa cagtagcggc cagtgctctg gctccgtcgg
      361 ggttgtgcgc accatccagg atcaccacaa acggagcgct cgctactacc tcgaatcgac
      421 cgggattggc ggcctgggcg aggcctcggc ggagatcatc aacaaatacg cacgccgacg
      481 tctttcgggc tccagccaac acgcccaggg ctgctgcggc agtggcacaa ttggaggcct
      541 gatgccggcc gacaaggccc gtggtggctc gaactgggcc gaatgacggc agatccatgt
      601 cgatggtagt gccgttcatg tcgattgata cgacatcgaa gcccacatcc ctgcccagca
      661 aggtcagcgg cgcacccgcg cgccgcgcgg cgtcctccag caccctcgtg acctcgggtt
      721 cctgcgggcc cactacaacc gacacgccgg gcttgatgat gcccgccttg tccgacgcga
      781 tctcgcctaa tgtgttccca agtttgtcca tgtggtccat ggctatatgg gtgatcaccg
      841 acacttccgg agtaatgacg ttggtggaat cgtacctgcc cccaagcccc acttcgacca
      901 cggctatttc cacttcttgg cttgcgaacc actggaagca ggctgctgtg gcgacctcga
      961 actcagtggg cgctccgata gaggggtcgc ccgcaaggcg ctgcaccgcc ggcgcaagtg
     1021 aagtaatgag cgcggccagc tcgcgcccgg gtatctcctg cccgcacacg cgaatgcgct
     1081 cggtatacct ttggaggtga ggagacgtgt acagccctgt cctaaagcct gccgccctta
     1141 gtacggattc tatcatcacc gccactgagc ccttgccgtt agtgccggct atgtgcacgt
     1201 gcttcatccg ctgctggggt tgacctgcga aatttagcag cgcgtcggtg cgttcgaggc
     1261 ctggcttcac tccgaacctg gccagggcgc ctaaatactc catggtctcg tcatagttca
     1321 tccatcatca cctgcgccgc ttgacggcgc gtatccagtc tcgccatcgg ccacagcctg
     1381 agccgcgtgg catgcccaat cgcattccga ttgtagcagg caccgagagc aggcaggacg
     1441 acctgccttg caggtcttgc gtccatgggt caccatgttc atgtgcagct gatacacgat
     1501 gtcgtcgggc accgctggca tcaataagga ctgggtagct gcaggacttc tggtggacac
     1561 caaccccagc cggttgctga cgcggtggac gtgggtgtct actgggaaga ctggccgacc
     1621 cagcgcgaac aggagcacac aggcggcggt cttcgggccc accccaggca gcgatgtcag
     1681 gtagtcgaag gcgtcagtgt ctcccatgtc cctgagcgcg gccaagctga tggaaccgaa
     1741 gtccgcctct atcctgcgca gagcctcgcc aatgcgggca gccttgatat cggccagccc
     1801 gcccatgcgg attacgtctg cgacacgcgt gggcggcgcc agggccagct gctcccataa
     1861 gggataggcc gccctgagcg ccgcgtaggt cctatcggaa ttagcgtccg acgtattctg
     1921 ggtgagaatg atgtgcacca actcgcccac ggggtcgcca tgaggcctca agagcttggg
     1981 cccatacatc ttcgccagaa tgccgtccac gcgtcttggc cgctcggcgc aggctgctgc
     2041 gatcgaggtt gcgtcggctg ccgcgtgcat ctcgtgatta gtgtccattt acgattcgag
     2101 ctgcgccact cggacccgca ctttggtcag ctggtcttcg agatcggcga gcctgtcgcg
     2161 ctgcttctgc acgacttcgg gcggagcctt caacacgaac gattcattgc ccagcttggc
     2221 ctggactttc tccgtctctg cctgcaggtt ctcgagctcg cggcggaggc gttcgatctc
     2281 tttgtcgata tcgatcatgc ctgccagagg cagataaacc tcgactcccg gcgccacggc
     2341 agcggcagct ttctcgggct tgggggccgc agccgattcc atcttgaccg ccgccaaccc
     2401 cgcaaggtgt gcaagcacgt cggcgctgcg gtcgagagcc gcggcgactt ggccgtcggc
     2461 atggaagatg gcatccacct tgcgcgaggg ggccacttcg aactcggccc gcacattcct
     2521 gatggcacgc acagtctcca tgatcatcgc catctccgcc tcggcctcag ggtcgagcca
     2581 tgcttcatcc atacgcggcc atgcctgaat catgatgctg tcgccttcgt gcggcaggtg
     2641 ctgccagatg ccttcggtta tgaagggcat gaacggatgg agcagcttga gcgctccgtc
     2701 aagcaccttc cacagcacat actgcgcggc gcggcggccc tgcttgtcgg cgtcgtcgta
     2761 cagccgcctt ttggcgatct cgatatacca atcgcaatac tcgccccaga tgaagtcgta
     2821 tatggttcgg gctgcctcgc ccagatcgta tctggccagc tgctgcgtga tctgcccggc
     2881 tacccggttg aagcgcgaca aaatccacct gtcggccaac gtcagctcaa ggtcgggcgg
     2941 aagctggccg cctgcgggcg cctcgaagtc gcccaagttc atcaagacga acctgctcgc
     3001 gttccatatc ttgttgcaga agttgcgcga ggcttcgacc ttctccgggc gccacctgaa
     3061 gtcgttgccg ggcgtattac cggtaatcag cgtgaaccgc agagtatcgg aaccatactc
     3121 ctcgatgatc tccaacgggt tgacgcctgt gcctttagac ctgctcatct tggatccatc
     3181 ggcattcctg atcaggccgt gcagcaatac atcggaaaac ggcttctcac ccatgaactc
     3241 cagggccata aagatcatcc tggccaccca gaagaagatg atgtcatagc ccgtcaccag
     3301 caccgacgtg ggatgccaag cggcaagctc gggagtgtgg tccggccagc ccatggtgga
     3361 gaaggtccat agcgcggagc tgaaccaggt gtccagcacg tccggatcct gctctacacg
     3421 ccctccgcac ttggggcagg ctatggcgtc ctcgcgtgag gcgaacatgt ggccgcagtc
     3481 atcgcagtac cacaccggta tgcggtgccc ccaccacagc tggcgcgaga tgcaccaatc
     3541 gcgcacgttc tccatccagt tgagatacat tttggtgaag cgctcgggca caaatcggat
     3601 actgccgttt ttcacggcct cgattgccgg tttggcgagg ggctccattc tcacaaacca
     3661 ttgcagtgac accaaaggct ctaccagcgt gccgcacctc tggcagtgcc ccacggcgtg
     3721 ggtatacttc tccacacgtt cgagcagccc cgccgactcg aaccaggcca ccacagcccg
     3781 cctgcattcc tcgcgtgtct ggcctgcgta ctccctagct tcgccggtca tgcggccgtc
     3841 tgggccaatc acgcagactg actcgagccc atggcggtgg gcgatctcga agtcgttagg
     3901 atcgtgggcg ggcgtcacct tcactgcccc tgtgccgaat gccgggtcta catgctcgtc
     3961 ggctacgatc ggtatctctc tgttcatgat gggcaacatt agggtcttgc ccaccagcga
     4021 cgcgtaccgc ggatcgctgg gattcacggc cacgccgatg tcgcccaaca tcgtctccgg
     4081 gcgtgtagtg gccacggtta catgtccggt cccgtcagtc agcgggtaac gcaggtacca
     4141 aagcgccgac tcctcgtctt cgtgctccac ctctacgtcc gacagagatg taccgcagtt
     4201 ggtgcaccag ttgaccattc tctctccccg gtagatgagt cccttctcga acagctgcac
     4261 gaacacctcg cgcaccgctc tcgagcagac tgggtccatg gtgaaccggg ctcgttccca
     4321 atcacacgac gcgcccagct tcttctgctg ctccacgatc tcgccgccgt gcttttcctt
     4381 ccagtcccag gcgatcttga ggaagccgtc tcggcccacg tcagtcttgg taaggccctg
     4441 tttggccagg cttgctacta ccttggcctc cgtggctatg ctggcgtggt cagttcctgg
     4501 aatccagcag gcattggcgc cctgcatcct cttggacctg ataaggatgt cctgaatagc
     4561 ggagtccagg gcatggccaa tgtggagcgt cccggtgatg ttgggcggcg gcatcatgat
     4621 cacgaaatgc tcgcgatcgg ggtctggatc cggctgggca tgaaagtagc cgcggtccag
     4681 ccagaactga tagatccgct cctcaatctg cttcggatcg tacgtagtgg gtatgtcggt
     4741 ctttcccatg tgcaatcctt cctccctgcg cctcgacgcg cagatgtgtt catgaatgtg
     4801 aaaacgccct gaccccggag ccaggggcat acacaaagcc tccttgccca tcaaagggcg
     4861 aaggaggctt cacttcgcgg taccaccttt gttctcaacg ggttgttcgc gcgcatcgcg
     4921 cacaccgtac gccggcggcg cgccgcactc gtcccgcatc gatctcggca gctgtaacgg
     4981 gctgatcccg accgggccta ctcttgccgg tcacgcgtgc ggccggcgat ttgggccagg
     5041 cggctcaggg gcgacattcg cgcggtcatg ggccaggacg ccttacaccc ggcggacgtc
     5101 cctctctgtg agcgtaaccc cgctactcct ccccatcatc gctatgcata tatgatattg
     5161 ccgttaacgc attctactca taccgcacgg gcaatgtcaa catgcgacta gccatgccct
     5221 gaaggtcagg actctttctc cttgtccttc ttggcctgtt cgatcacctt ctcggccaca
     5281 ctgaacggga cctcctcgta gtggtcgaac tccgtcgtga acgttcctcg gcccgccgtc
     5341 atcgagcgca ggtcaataga gtacttgaac atctcggcca tgggcactag ggcgcggacc
     5401 acctgatacc tgccctctgg ctccatgccc agaatccggc cgcgcttctt gttgaagtcg
     5461 cccatcacat cgcccatgta ctcctcaggc accgtcacct cggcccggac aatcggctcg
     5521 agaataatcg gattagcatc ctgacagcct ttcttgagcg ccagttgagc ggcgatcttg
     5581 aaggccattt cggacgagtc cacaggatgg ctggaaccgt cgtacagcga gcacttcagg
     5641 tctaccagag ggaagcctgc cagcgcgccg ccttcgggaa ggccctctct gagccccttc
     5701 tcaacagctg ggatgaactg tcgggggaca gcgcctccaa agaccttgtc ttcgaactcg
     5761 aagccagagc cagtcggcag cggagacatc tcaatccaca cgtgcccgaa ctggccacgc
     5821 ccgcctgttt gcttcttatg cctgccctca accttaaccg tgcccttgat agtctccctg
     5881 tagggcacct tgggggtgtc cagagtcatc tcggcgccga acttcctctt aagcctctca
     5941 gcaatgatgt caaggtgaac ctcacccatg ccagctagaa gcagctcgcc cgtctccgct
     6001 tccttggtat atcggagagt agggtcctcc tcctgcagcc tagcgagccc ctgaccgatc
     6061 ttgtcctcgt cgcccttgga cttggccctg gcagccatgg acaacaccgg cgatgggaac
     6121 tttatgggat cgagtacgac cggcttgtcc ttgtcggaaa acgtgtcgcc ggtagtggtc
     6181 tcgctcagct tagccacacc ggcaatgtcg cccgcgccaa cctcagccac tgcaatctgc
     6241 tgcttgccct tgatcaagaa cacctggcca aagcgctcga tccgatcctt gctgacgttg
     6301 tacacctgag aatcggactt aactacgcct gagtagatgc ggaacatggt tatcttgccc
     6361 acgtaagggt ctgccatggt cttgaagaca agggccgaga acggttcggc ctcatccgga
     6421 cgtcgctcca cactatcctc agagccgggc aagcggccca ttattgctcc ctgagccaca
     6481 ggcgacggaa gaagcgcaac tatcgagtcc atgagcggtt ttacacccac catgcgcagc
     6541 ccagatgcgc agaacacagg gactaccgag ccgtcggcta ctccggccgc caggcccctc
     6601 tcgaactcct cctgcgtgag ctcctcgccc tccagatact tgttcagaag ctcctcatcg
     6661 ctctcagcag cgtactcaat gagcttctcg cgcagctccc cggcggcatc agccagctca
     6721 tccgggatcg gtgactcggc gacgttcttg cccgaagcat cgctccagat gaaagccttc
     6781 atgtgtatga tgtccgccac accgcggaac ttatcgcctt cgcctatagg aagctgagcc
     6841 gcgaccgcgt tggtcccaaa gatctcacgt atggagtcaa gagccttgcc aaagttggca
     6901 ttctctctat ccattctgtt gatgactatt atacgcggaa gcttgcggtc atccgcatgc
     6961 cgccaggtca gttctgtgcc cacttccaca ccggaaaccg catccaccag caccattgcg
     7021 gcgtccgcta tgcgcaatgc gcccacgacc tcacccacaa agtcggagta tcccggcgta
     7081 tctatgaggt tgatcttgtg gccgttccac tccacaggcg cgacagaggc gttgatggag
     7141 atcttacgct tgacctcatc aggatcgtag tccatcgtgg atgtgccgtc gtccaccttg
     7201 ccgaagcgat caaccgcgcc gcccaagaag agcatggcct ctgcaagagt cgtcttgcca
     7261 gccccaccgt gagagataac ggccacattg cgcagttggt ccactctgta tttcttcaag
     7321 cgatctcctc ccttaaacgc ttcactcgct acccgggcct ttcaccagct ggtgaaggca
     7381 gtatagatat ggctctcgaa gtaccttgcc aggttcttca gccgttatgc cccggtttcc
     7441 ggttgttcac tggaagacgc ggcagatgcc gctggcagtc atagatgctc actctaatat
     7501 tttatacttc gtcatggacg taggcaatag cgaatctccg ggcggaccgc gtcaatctac
     7561 agtaggaaag aagagctaag aagctattcc gtagtgct
//
LOCUS       DAOTAC010000101         7571 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_8271_length_7571_cov_5.075306, whole genome shotgun sequence.
ACCESSION   DAOTAC010000101 DAOTAC010000000
VERSION     DAOTAC010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7571
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8271_length_7571_cov_5.075306"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..202
                     /locus_tag="PLM74_05640"
                     /pseudo
     CDS             1..202
                     /locus_tag="PLM74_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019584040.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     gene            339..1079
                     /locus_tag="PLM74_05645"
     CDS             339..1079
                     /locus_tag="PLM74_05645"
                     /inference="COORDINATES: protein motif:HMM:NF025559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HON42602.1"
                     /translation="MQNRNAASQPSEGTDALLPGHHALLFSALARSILSTCGEAAEAI
                     LTEAVKSYALERGKRMRKRAIANGDGTSMASYRAYCEWTAPPGSISSIVAARSPVLIT
                     HTLVCPWYEAWKVTGRLREGAFYCRYVDEYLVRGFDESLVLEVKTMHDSSCPRRCEFR
                     WNGIDLTQEEEVRIDAMRARVEQQGCVKDWRYHMGHLISSFAGSLRRHGIDESTVLIP
                     ARADFASLCGEAAASVFDDYNDVDFDAE"
     gene            complement(1102..1326)
                     /locus_tag="PLM74_05650"
     CDS             complement(1102..1326)
                     /locus_tag="PLM74_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42603.1"
                     /translation="MTLAIPGLRPPLLSSHTAALLDKYRAFRHVFRNIYGFRLDAERT
                     PELVRGLHKTVLALSSDLDQFVSGVDRAIQ"
     gene            complement(1480..>2107)
                     /locus_tag="PLM74_05655"
     CDS             complement(1480..>2107)
                     /locus_tag="PLM74_05655"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HON42604.1"
                     /translation="MGVITVKGRGCVSAQPDVVTLSFTTGSKARDYAECVDSLNGRTA
                     ALRRDLAASGAECAELKTADFRVDVEYREEKGRYKFDGYSASHRLEIELPADKGMLNR
                     VLGAVAQGNSGAEIRIRFSVKGIESLRRQALAEAVKVARLSAESLAQAAGATLGKLQS
                     IAHGWDEIRLSGPAYGVVCERVSDYAVDIDPKDVSVSEDVTLVYEIVE"
     assembly_gap    2108..2207
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2976..4058)
                     /locus_tag="PLM74_05660"
     CDS             complement(2976..4058)
                     /locus_tag="PLM74_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON42605.1"
                     /translation="MAKGIQLEGVSKIFRHPERGDVHAVEQLNLAVQPGELVTLLGPS
                     GCGKTTALRMIAGFENPTTGRILIGGQDVTNIMPNRRNIGFVFQNYALFPHMSVFDNV
                     SYGLAVKGISMAERRDRVAHVLDLVGLQGMERRYPNQLSGGEQQRVALARVIVTEPSV
                     LLLDEPLSNLDAKRRVQMRSEIRRLQRSLNMTSVYVTHDQEEALSISDRVVVMNSGMV
                     EQEGTPQEIYANPASLFVAGFIGKVNALPAEVLRVDRDEVTASFGATNFVVCRPKMSI
                     SAGQRVSLLIRPESVDLSPEPDGPAPRGRITQVTYLGEKVEYDIECDGTQIKAVSFDP
                     ARHGMFLQGQDVYLHMQGGSMILTDQ"
     gene            complement(4076..5791)
                     /locus_tag="PLM74_05665"
     CDS             complement(4076..5791)
                     /locus_tag="PLM74_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HON42606.1"
                     /translation="MSSTSAIMRREKRRIELANLARDPVLLLTVVLVWVAMFIFILWP
                     LLKLMQAAFVDKGHLSAKALVSILSRPNNRLALRNSLLLGGLVALIGTALGYLFAFTY
                     TRTNISAFWRGMIDVACLLPLISPPFTLALAILFALGPRGLITYGILGLKNVNLYGLQ
                     GALLAEVLTYFPIAYLTLRGILANVDLSMEDAAFSLGASRLRVFLTVTLPLTIPGIAN
                     ALLVLFASSLADFASPLILSGARFPVLPTQAYLQITGLYDMKGGAALCFLLLVPSLLI
                     FAIQRQWVGKRSYVTISGKSAASGDGRLAPPWVSGLLLALCLAISAFVFFLYGSILFG
                     SLVRAWGANHTFTLENYRYVFTFGRKAIRDTLIIALTAMPIGGIYGILVGYLVSRKSF
                     PGRSAMEFVSMINYSLPGIIVGIGYLLAFNNPPIALAGTAWILIAAYVFRYSATGIRA
                     TVATLHQMDPAIEEASTSLGAGTAVTFRKITVPLIAPAFFAGLEIMFIRAMTAISATI
                     FLISVNWTLITVRILEGATELELGHAAGFSMLVIAIVFITMGILRWIMRRAGMSEYSA
                     VAQIR"
     gene            complement(5882..6892)
                     /locus_tag="PLM74_05670"
     CDS             complement(5882..6892)
                     /locus_tag="PLM74_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HON42607.1"
                     /translation="MSRSRTTVLAAALLGILLLAVSGVGGASVLDVYSIWPESFCTPM
                     FQEFTAKTGIKINFVRHSSGEVLARIIAEKDNPRVGMVFGGPADTFAAAIDEGVLEPY
                     IPAGVRAIPEKYRHPKGYYTGIALNPIVFLSNRSFLADNKLKAPSSWNDLLNPAYRQQ
                     LQMADARTSGTGVNRIISTIFAMGSEDAAYKYMKELDKQVQVYTKSGGGGTVPVGTRQ
                     AGAGVFFLVDCILTQQKGYDVAITFPKEGSAVALEACALIKGGKNHDLAKKFMDWLVT
                     AEAQSLYAKHGIGFMPTNPDAKVSELIDVRGANFVDVDLQWAADNRQRLIDRWVTEVL
                     GQ"
BASE COUNT         1582 a         2263 c         2089 g         1537 t
ORIGIN      
        1 cacccggctg cgcgacctcg gcaacaccgt gatcgtcgtc gagcacgacg aggaggccat
       61 ccacgccgcc ggccatgtcg tggacatggg cccgggcgcc ggggagcatg gcggccgcat
      121 cgtcgcggaa ggcacgccgc agcacatcgc cgcccacccg gattcgctca ccggcgccta
      181 cctgtccggc cgtcgcgcca tccagaccat ctgcaggctc gacggaaagc ggtttcggac
      241 aatggtcgat taggtgtgat cgaaggccct gaccggtatc tctgcagcag ttcgatgcta
      301 tcagagtatt ctggcgctca acgggaggat tgatgaccat gcagaacagg aatgcggcaa
      361 gccagccgtc agagggcacc gacgccctgc ttccgggcca ccatgccctg ttattctccg
      421 cccttgcgcg gagcatactc tctacctgcg gagaagctgc agaggccata ttgacggagg
      481 cggtcaagtc gtacgctctc gagcgaggca agcgcatgag gaaaagggcc atcgccaacg
      541 gcgatggcac ctccatggcg tcatacaggg cctattgcga atggaccgcc ccgcccggat
      601 ctatctcgtc catcgttgcg gcaaggtccc cggttctcat tacgcacact ctcgtctgtc
      661 cttggtatga ggcatggaaa gtgactgggc gactgcgcga gggcgcgttc tactgcagat
      721 acgtcgacga atacctggtc aggggtttcg atgagagcct ggtcctggaa gtcaagacga
      781 tgcacgactc ctcatgcccc cgccgttgcg agttcaggtg gaatggaata gacctaacgc
      841 aggaggagga agtccgcata gacgcgatgc gcgcccgcgt agagcagcag ggttgcgtta
      901 aagactggcg ttaccacatg ggccatctca taagctcctt cgcgggttcg ctaaggaggc
      961 atggcattga cgaatcgacc gtgctcatac cggcaagggc cgacttcgca tcgctgtgcg
     1021 gggaagccgc agctagcgtg tttgatgatt acaatgacgt tgacttcgac gctgaatgag
     1081 cagtgacggc cagatgcgcc tctactgtat cgcgcgatca actccggata cgaactgatc
     1141 caggtcggag ctgagcgcca acaccgtctt gtgtaggccg cggaccagtt ccggagtccg
     1201 ctcggcgtcc agccgaaatc cgtagatgtt cctgaacaca tgcctaaatg cacggtattt
     1261 gtccagaagc gctgccgtgt ggctagacag caacggagga cgcaatccgg gaatggccag
     1321 cgtcatttga cctaacagct ctttgcgcga gtcgtcgccg ccgagaacac ctcctcaaaa
     1381 gacaggctat tgcagcagag tgtttccacc atcgctgggg caattcctgc atcgccaaat
     1441 gcgcggtgca tctcgtgttc agctatgtct gtgcggcgtc tattctacga tctcatatac
     1501 cagcgtcaca tcttcgctga ccgacacgtc tttcgggtct atgtccactg cataatcaga
     1561 aacgcgttcg cataccacgc cgtaggcagg cccggacagg cgaatctcgt cccagccgtg
     1621 cgctatgctc tggagcttcc ccaacgtcgc cccagccgcc tgtgccagcg actcggcact
     1681 gagccttgcc actttgacgg cctccgcaag agcctgccgt ctcagactct ctatgccctt
     1741 gaccgaaaag cggatccgta tctctgcgcc gctgtttccc tgggcaactg cccccaacac
     1801 gcggttgagc atgcccttat cagccggcag ttcaatctcc aggcggtgcg aggccgaata
     1861 cccgtcgaac ttgtaccggc ccttctcctc gcgatactcg acatccacac ggaaatcggc
     1921 agtcttcagc tctgcgcatt cagcccccga tgctgccaga tcccggcgca gggccgccgt
     1981 gcgcccgtta agactatcca cgcactccgc gtaatcccgc gccttgcttc cggtagtgaa
     2041 cgacaacgtc acaacatccg gctgcgccga cacacagccc cttcccttaa cggtgatgac
     2101 gcccatgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnctc cctacgagaa
     2221 aacgggttcg cccgctgggc accgaggcga agttgggact accgaagaaa aaggcgcggg
     2281 ctgcaggcaa cttgcatggc agccgacagg cttcccgtca aacctgtagg ccacacagta
     2341 tgcgtcgccc gcatccatgg aaaggtccgg gcgtaagggc ttgctcatct tgaacctctg
     2401 tctattcccg ctcgggcgga ggcaggggcg tctggcggag cagtgtgatg cgaggcctcg
     2461 atggggaaac aggcatcaca cagggatttg ctgagcctga ggccgcttgc tctcgaagtc
     2521 ctcgccgata aggcgcttct agctgggcct gcagagattt gcactctccg gggctcaacg
     2581 cggaaatctc ctgccgtgtg agtgcagaaa atctccgccg tgctggttta gggccgcctg
     2641 aatgccacaa cgcggcattt gctgcgccat tcccatgccg ataatagcca tatatcctca
     2701 gcatcatgcc gacatacaat atctaggcat aggaggacgc ctcgttcgtt gctgtatacc
     2761 gtttgttggc ggcccatcgg cagaataagt aatccaggct gacctgcgcg gaaattcttc
     2821 gccgcatgtg tgcagaaaat ctccgctgaa cgcctccaga ggggagaaaa gctgcccgga
     2881 tacccccttc gggtatccat catggcatcg ccccgaaatc gcctcggtct atcggcatca
     2941 ggccctaaac tcacccatcc catgctgccc gattactatt gatccgtgag tatcattgat
     3001 ccaccctgca tatgaaggta cacatcctgc ccctgcagga acatgccgtg gcgggccgga
     3061 tcgaaactca ctgccttgat ctgggtgccg tcgcactcga tgtcgtactc cactttctct
     3121 ccgaggtaag tcacctgtgt gatacgccct ctgggcgcag gtccgtccgg ttcagggctc
     3181 aggtctaccg attcaggccg gatgagcagc gatacacgtt gtcccgctga tatcgacatt
     3241 ttaggcctgc acaccacgaa atttgtggcg ccgaaggaag cagtgacctc atcgcggtcc
     3301 accctgagca cttcggcagg cagggcattc accttcccta tgaaacccgc aacgaacagt
     3361 gatgccggat tagcgtatat ctcctgcgga gtgccttcct gctcgaccat gcccgagttc
     3421 atgaccacaa cccgatcgga aatgctgagc gcttcttctt ggtcatgggt cacgtacaca
     3481 cttgtcatgt tcagagagcg ctgcagacgc ctgatttccg agcgcatctg cacacgtctt
     3541 ttcgcgtcca ggttggacag aggttcgtca agcaggagaa cactgggctc ggtcactatg
     3601 acccttgcaa gcgccacccg ctgctgctcg cctcctgaga gctgattggg ataccggcgt
     3661 tccattccct gcagcccaac cagatcgagc acgtgagcga ctcgatcgcg cctctcagcc
     3721 atggatatgc ccttcactgc aagcccgtat gacacgttat cgaacacgct catgtgtggg
     3781 aacaacgcgt agttttggaa cacaaagcct atgttccgtc tgttgggcat tatgttggtt
     3841 acatcttgac cgcctatcag tatgcgtccg gtggttgggt tctcaaatcc ggcgatcatc
     3901 ctgagcgcgg tagtctttcc gcagccgctc ggcccgagca gggtcaccag ttcgccgggc
     3961 tgcacagcca ggttcaactg ctcaaccgcg tgtacgtcgc cacgctccgg atgcctgaat
     4021 atcttgctta ctccttcgag ttgaatgccc ttagccattt tctgaccccc tcgtatcagc
     4081 ggatctgggc aaccgcagag tattcgctca tgcctgcgcg acgcatgatc catctgagga
     4141 tgcccatggt gatgaagact atcgctatta ccagcatgga gaagcccgcc gcatgaccaa
     4201 gctcaagctc agtggcgcct tccaggatcc taacggtaat aagagtccag ttcacggaga
     4261 tcaggaagat agtcgcgctg atagctgtca tggccctaat gaacatgatc tcaaggcctg
     4321 cgaagaacgc cggcgctatc agcggcacgg tgatcttcct aaaggtcact gcagtgccgg
     4381 cgcccaggct cgtcgacgcc tcttctatcg ccggatccat ctggtgcagt gtggccacgg
     4441 tggcgcggat gccggttgcg ctgtatctga atacataggc ggcgatgagt atccacgcgg
     4501 tccccgccaa tgcgatggga gggttgttga aggccagcag atacccaatg cccactatta
     4561 tcccaggcag actgtagttg atcatcgaca caaactccat tgcgcttctg cccgggaacg
     4621 atttgcgcga caccaagtag cccacaagta tgccatagat gccgccaatg ggcatggctg
     4681 tgagcgctat gatcagggtg tctcggatgg cttttcggcc gaaagtgaag acgtacctgt
     4741 agttctcaag cgtgaatgtg tgattcgctc cccacgctcg caccagggac ccaaagagta
     4801 tcgagccata gaggaagaat acgaaagcag atatggccag gcacagggcc agcagcagtc
     4861 cgctgaccca cggcggcgcc aaacgcccgt cgcctgaagc cgccgacttg cccgaaatgg
     4921 tgacgtagct cctcttgccc acccattgcc tctggatcgc aaaaatcagc agcgagggca
     4981 caagcagcag gaagcatagc gccgccccgc ctttcatgtc gtaaagcccg gtgatctgca
     5041 ggtaggcctg ggtaggcagg actgggaaac gggcccccga caggatcaac gggctggcga
     5101 agtccgcaag cgagctggcg aacagcacca gcaatgcgtt ggcgataccg ggtatcgtca
     5161 gcggcagtgt aaccgtaaga aacaccctaa gcctcgacgc tcccaggctg aatgcagcat
     5221 cctccatgct gagatcgaca ttggcaagga tgccgcgcaa ggtgagatag gcaatcggga
     5281 agtaagtgag cacctcggct aggagggctc cctgcagccc gtagaggttc acgttcttta
     5341 ggccaaggat gccgtacgta atcagccccc taggcccgag ggcgaacagg atggccagag
     5401 ccagcgtaaa cggaggcgat atcagaggca gcaggcaagc cacgtcgatc atgcctcgcc
     5461 agaaagcgct gatgttagtt ctggtgtagg tgaaggcaaa cagatagccc agcgccgtgc
     5521 cgattaaggc gaccagccct ccgaggagca ggctgtttct gagtgcaagg cggttgttgg
     5581 gcctcgacag tattgatact agggcctttg ctgacagatg ccccttgtct acaaatgcgg
     5641 cctgcatgag cttgaggagg ggccatagga tgaatatgaa catggcaacc cagaccaaga
     5701 ctaccgtgag cagcagcacc ggatcgcgcg cgaggtttgc caattcgatg cgcctcttct
     5761 cgcgacgcat gattgcggac gtagacgaca ctccattccc tccctcaaga ggcggccttt
     5821 cggccgcctc tcacggatgc gcatagtgcg tatgacgcta agcgggttgc cccgctgaca
     5881 actactgccc tagcacctca gtgacccagc ggtcgatcag gcgctggcga ttgtcggctg
     5941 cccactgcag atcgacgtcg acgaagttgg cacccctgac atcgatcagc tctgatacct
     6001 tggcatcggg gttagtgggc ataaagccta tgccgtgttt ggcatagagg ctctgcgcct
     6061 cagcggtgac cagccagtcc atgaacttct tggccagatc gtggttcttg ccgcccttga
     6121 tgagtgcgca ggcctcaagg gcgactgcgc tgccttcctt ggggaacgtg atggccacgt
     6181 catagccctt ctgttgagtg agaatgcagt ctaccaggaa gaatacgccc gcaccagcct
     6241 gccttgtgcc taccggcacg gtgccgcctc cgccgctctt ggtgtagacc tgaacttgct
     6301 tatcgagttc cttcatgtac ttgtacgccg cgtcctcgct gcccatggcg aatatggtcg
     6361 atataatcct gtttacgcct gtgcccgatg tgcgcgcatc agccatctga agttgctgcc
     6421 tgtaggcagg attaagcaga tcgttccatg aggacggcgc cttcagcttg ttatcggcga
     6481 ggaagctgcg gttggaaaga aagacaatgg gattcagcgc tatgcctgtg tagtagccct
     6541 ttggatgacg gtatttctca gggatcgctc gcactcccgc aggaatgtag ggctcgagga
     6601 caccttcatc aatggccgct gcgaaggtat cggcgggccc gccgaagacc attccgaccc
     6661 gggggttgtc cttttcagct atgatcctgg caagcacttc gcctgacgaa tggcgaacga
     6721 agttgatctt gatgccggtc ttggctgtga actcctggaa catgggcgtg cagaacgact
     6781 ccggccagat gctgtagaca tccagcaccg aagctccgcc tacgccgctc acggctagca
     6841 ggagaatgcc tagaagagcc gccgcaagaa cagtagttct ggaccgtgac atttgatacc
     6901 aaacctccta gcgcagttgc ctgtcctaat tcacttcttc atagttctac acgacggtgt
     6961 gaacccctgc agaaagaccg aatcttccgg aagggatcag aagagcctaa atagccttct
     7021 cagttgcact cgagaatgcg catcgaacgc taggcagacc caccgcttgt tacgccctgt
     7081 aagccatttt gctatcatca tgcacagagc aaggggaggg ggagcgaaat gccgcagtat
     7141 gtcatcgcct agttcttggg tgatcaccgc gactccagta tagctgcggg gttcgagttg
     7201 cctgatacac acgctgtcac gcacactcat tgctgcatac tctcccccaa aggcccatag
     7261 agttcgcccc tttactacgc gctgaactgc ggttcctcga ccaccgtgtc agcttatcag
     7321 cgccggcgcc tgcgagcgcc acgtaacctg ggatctcgcc tgtgtataag gttcgctcac
     7381 ctgtaatata tgcatcgcag cctttctgaa aagcctccct aacctccgag gtcaatcctg
     7441 atcccccgca gaccagccca acccttcgta tcaataggcc gtcactcgat gaatacggct
     7501 gagtaccaat taggagtctc ctctcgagtc ttcagagtgt cgagcggcaa gccgaactgg
     7561 cgcacagtct t
//
LOCUS       DAOTAC010000102         7547 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_8295_length_7547_cov_4.493727, whole genome shotgun sequence.
ACCESSION   DAOTAC010000102 DAOTAC010000000
VERSION     DAOTAC010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7547
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8295_length_7547_cov_4.493727"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="PLM74_05675"
     CDS             <1..450
                     /locus_tag="PLM74_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HON42608.1"
                     /translation="TESDFQATLDAVREARFDAAFTFIYSPREGTRAASMPDQVPEDV
                     KHDRIYRLIELVNATAEDVNESLRGRVVEVMVDGPSERDPSILAGRTRSNKMVHWRPV
                     PCGLDSADGQGGVLAPGQLAMVRVLEPRTWTLHGEDAGVPREAGQVD"
     gene            450..>2179
                     /gene="mutS"
                     /locus_tag="PLM74_05680"
     CDS             450..>2179
                     /gene="mutS"
                     /locus_tag="PLM74_05680"
                     /inference="COORDINATES: protein motif:HMM:NF003810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HON42609.1"
                     /translation="MANKPTPMIKQYRELKAEHPDAILMFRLGDFFEMFCEDAEIASR
                     ELGIVLTSREAGPSNKMPMCGVPHHAVDGYIATLVQNGHKVAIADQVEDPRVARGLVR
                     REIVRVITPGTLADQVDPVTNNFLIAVVVAPTGMGLAACDASTGELLATQVLGDDAAK
                     VISEEIGRISPSECIYPGDDSEISAKQPCPVEGEFVSYLRPRRDFELFRAEETLLGQF
                     GVTSLEGFGARGMDLAVRAAGGLVSYIQETQMSRAPQISRMRVYHLGEFMQLDGPTRR
                     ALDLFGRERGLVKALDDTRTPMGGRLVRAWLERPSVSAAEINARLDSVEELSGDTFMR
                     ADVRAALDRMYDLERTCGRIGYRTVGPRDLLALAASLEAAAGLREVMAGAVSLRLVEL
                     RSAIDPVPDAVSRVRESIRDDAKAAIGEGGIIRPGFSAEVDELSRASTEGRQWLVKLE
                     AGERERTGIRSLKVGFNQVFGYYIEVTHANSHLVPAEYTRKQTLANAERYITPELKEL
                     ESKILGAEERLVRLEAAIFQQVVDEVAEYLPRVLCTARAVAQVDVLAAFAEDARDSGY
                     VRPVVDDGCVT"
     assembly_gap    2180..2189
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2190..3060
                     /locus_tag="PLM74_05685"
     CDS             <2190..3060
                     /locus_tag="PLM74_05685"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HON42610.1"
                     /translation="TRIREARHPVLERILGPSRYVPSDLEVGEGGGQLLIVTGPNMAG
                     KSTVLATVGLITVMAQIGSFVPAREARIGICDAIFYRTGSYDDIGSGKSSFMVELLET
                     ANILNTATNRSLILVDELGRGTSTYDGMALAWAVAEWIHDQVAARTLFTTHYHELAAL
                     EERLPHTRNYHVGAVEQGGELVFLYRLARGSVDRSYGLNVARMAGLPREVIRRAQQIL
                     RGLESAQPSGGHQMTLFGWADEEPLPIATAEARSDGGVGGRVMELLDSIDPDSLTPRE
                     ALSLLYELKRMQE"
     gene            3071..4879
                     /gene="mutL"
                     /locus_tag="PLM74_05690"
     CDS             3071..4879
                     /gene="mutL"
                     /locus_tag="PLM74_05690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HON42611.1"
                     /translation="MRILKLAPEVADRIAAGEVVERPSSVVKELVENSIDAGARAVRI
                     EIAGGGLEMISVSDDGCGMSREDASLAFERHATSKISRAEDLFSIVTLGFRGEALPSV
                     ASVARVRLVTRERGAAHGTVVTAEGSVVSGPSAVGAPEGTTVTVRDLFYNTPARLKYL
                     RSVATETRRVIETVGRLALAHPEVSFSLDVDGRNVLSTPQGGSLREAAAAVLGIELAR
                     SMAPVDKTTHGITVRGLAGPGDCAKSSRDVQHVLVNGRPASCKTVWAAIDRAYDRSTP
                     PGKKAPVVLSITVDPSSVDVNVHPAKSEVRFASESDVYSAVYGAVSAALRSMDAVPDI
                     GSKMRDGEQPGRGVELDDDASRRGSAAGARLWPSIAADRREGPAYGGGRQAEYDTSYG
                     KNADYGTGAGNVACERSVPVTIIGQYADAYILAESDEGMLIIDQHVAHETVLVDTYRR
                     QLASASSGPVTQLLLSPELVQLGAADTDLVARHQSTLARMGFEADVFGVASVLVRGIP
                     IAAADMTPGEALGAAIEGIAAASAEADPESLADHIAVSLACHSAVKAGAHLHEEEMKS
                     LVAALFRCENPMRCPHGRPVIVTVSKESLERSFGRR"
     gene            4876..5736
                     /locus_tag="PLM74_05695"
     CDS             4876..5736
                     /locus_tag="PLM74_05695"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HON42612.1"
                     /translation="MIITRGLVVTTSSRAGASEIERARKVASALNSPYIERHSKSVAQ
                     VAKAAEVAREWPGPGKVEGVLVVESSRMTLVGMGQSGPAGPRLFFHPGMGPGRIRRAA
                     QGRQDHMLEAMGLGRGDRVLDCTLGLASDAIVAAWAVGSSGKVVGIESSPALAEIVRA
                     GLREYDKADPDTTDAMRRIEVVCADHLMHLMSCPDQSYDVVYFDPMFREPVRLSPGIA
                     GLRRWACRSRLSGNAVREATRVARRRVVIKDRSEGSELDRLGVTMRLGGRGSRVEYGV
                     ICLTDEVTCR"
     gene            5733..6722
                     /gene="miaA"
                     /locus_tag="PLM74_05700"
     CDS             5733..6722
                     /gene="miaA"
                     /locus_tag="PLM74_05700"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HON42613.1"
                     /translation="MTWPMRIPLLVIVGPTASGKSAVGMALARRLDGEIVSADSMQVY
                     RGMDIGTAKPTPSEQAEIRHHLIDVADPDEVFNVARYQELASAAIDDAAVRGKLPIMV
                     GGTGLYVDAVVRGFLFPDEGRNSAVRARLEQEAAGEGGAPTLHARLASCDPEAARRIH
                     PNDLRRIVRALEVFEVTGRPITELRRKHVPVNAYSARLFGLTMPREALTERIDARVDQ
                     MVAAGLVDEVRRLLQMGYDGQSTAMQAIGYKEFAAYLAGREGLEQAIDEVKLETRKYA
                     KRQMTWFRKHDDITWIDVQVASDPSDVASEIMARLGDWLEEADTALGRTQGRH"
     gene            6697..>7547
                     /locus_tag="PLM74_05705"
     CDS             6697..>7547
                     /locus_tag="PLM74_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HON42614.1"
                     /translation="MDERRDDINDNDDTNRNDVTNGNYVMNGNYDTSNLDDREIARAL
                     GLNDEWLDAAGRIRARIAPEVAVTDKIARVNSARVLAALGRAGLGDYHFGGTSGYGYD
                     DTGREHLERAFADVFGAEAALVRIQMVSGTHALAAVLFGVLRPGDLLVSAYGSPYDTM
                     VTMIGHDRRVRGSLAEHGVEYLEVPAGEGGEPDYAGISRAAEMARMVLIQRSRGYSWR
                     RALSCENIGRIARCVKAANPRAVVFVDNCYGEFTETMEPCAVGADVVAGSLIKNPGGG
                     IAPCGGYV"
BASE COUNT         1439 a         2089 c         2635 g         1374 t
ORIGIN      
        1 acggaaagcg acttccaggc aacgctcgac gctgtgcggg aggcccgatt cgatgccgcg
       61 ttcactttca tctactctcc cagggaaggc actcgggctg cttccatgcc ggatcaggtg
      121 ccggaggatg tgaagcacga caggatatac cgcctgatag agctggtgaa cgctacggct
      181 gaggacgtaa acgagtcatt gcgggggcgt gtggtcgagg tcatggtaga cggaccgtct
      241 gagcgtgacc cttccatact agctggcagg accaggtcca acaagatggt gcattggcgc
      301 cccgtcccct gtggcctcga tagcgccgac ggccagggag gcgtgctagc cccgggccag
      361 ttggcgatgg tgagggtact agaacctcgc acgtggacgc ttcacggcga ggatgccggt
      421 gtgccgcgag aggcagggca ggtagactga tggccaacaa gccgacgccg atgatcaagc
      481 agtatcgtga gcttaaggcg gagcatcctg acgccatcct catgttcagg ctgggcgact
      541 tcttcgaaat gttctgcgaa gatgcggaga tagccagtcg tgagctgggt atcgtcttga
      601 cgagcaggga ggcaggcccg tcaaacaaga tgcccatgtg cggcgttcct caccatgcag
      661 tggacggcta catcgctacg ctagtacaga atgggcacaa ggtagctatc gccgatcaag
      721 tggaagaccc tcgcgtagcc cgaggccttg tgcgccgtga gatcgtgcgc gtaatcactc
      781 cgggcactct ggccgaccag gtggacccgg tcacaaacaa cttcctcatc gcggtggttg
      841 tggccccgac aggcatgggg cttgcggcgt gcgatgcatc caccggcgag ctcctggcca
      901 cccaagtgct gggtgatgat gccgcgaagg tcatatctga ggagataggc aggatatcgc
      961 cgagcgagtg catctatccc ggagacgaca gcgagatatc cgccaagcag ccatgcccgg
     1021 tggagggcga gttcgtgtcg tacctgcggc ctcggaggga ttttgagctt ttccgcgccg
     1081 aagagacgct tctcgggcag tttggcgtga cttcgctgga aggcttcggc gcccgcggaa
     1141 tggatctggc tgtccgtgcg gccggaggcc tcgtttcgta tattcaggaa acccagatgt
     1201 cccgagcgcc gcagatatcg cgcatgcgcg tctaccatct gggcgagttc atgcagttgg
     1261 acgggcctac tcggcgggcc ctggatctgt tcgggcggga gaggggcctt gttaaggcgc
     1321 ttgacgacac gcgcacgccc atgggcggaa ggctggttcg cgcctggctg gagaggccgt
     1381 ccgtgtcggc tgccgagatc aacgcacggc tcgactccgt tgaggagctg tcaggcgaca
     1441 cattcatgcg ggccgatgtc cgcgccgcgc tggaccgcat gtacgacctg gagcgtacat
     1501 gcgggcgtat cggctacagg acggtgggcc cccgggacct gctggccctg gctgcatcgc
     1561 ttgaggccgc cgcaggcttg cgggaagtca tggctggggc ggtttcgctg cgtcttgtgg
     1621 agcttcggtc ggctatcgac ccggttcccg atgcggtgag cagggtccgc gagtccatac
     1681 gggatgacgc caaggccgcg ataggcgagg gcggcatcat ccgcccgggg ttcagcgcag
     1741 aagtggacga gttgagtagg gcgtctaccg aggggcgaca gtggctcgtc aagctggagg
     1801 ccggcgagcg cgagcgtacc gggattaggt cgctcaaggt cggtttcaac caggtattcg
     1861 gctactacat agaggtgacg cacgccaact ctcacctggt gcccgctgag tacacccgca
     1921 aacagacact ggccaacgct gaaagatata taactccgga gctgaaagaa ctggagtcca
     1981 aaatcctggg cgcagaagag cgcctggtgc ggttggaggc ggcgatattc cagcaagtcg
     2041 tggatgaggt ggctgagtat ctgcccaggg tactgtgcac ggcgcgtgct gtggctcagg
     2101 tcgacgtgtt ggcggctttt gccgaagacg cgcgggactc gggatacgtg cggcctgtgg
     2161 tggacgatgg atgcgtcacn nnnnnnnnnc actcggatcc gcgaagcgcg ccatccggtg
     2221 ctggaaagga tcttggggcc gtctaggtac gtgccgagcg atctcgaagt gggcgagggc
     2281 ggaggacagt tgctcattgt cactgggccg aacatggcgg gcaagtccac agtacttgct
     2341 accgtggggc tgatcacggt gatggcccag ataggatcgt tcgtgcccgc ccgggaggcg
     2401 cgcataggca tatgcgacgc catattctac cgcaccggtt cctacgacga cattggctcg
     2461 gggaagagca gcttcatggt tgaactgctc gagacggcca acatcctgaa cactgcgaca
     2521 aaccgcagcc tgatcctggt ggacgagcta ggcagaggca ccagcaccta tgatgggatg
     2581 gcccttgcat gggctgttgc cgagtggata cacgaccagg tagcggcgcg cactctgttc
     2641 accactcact accacgagct tgcggcgctc gaagaacgtc tgccgcatac gcgcaactac
     2701 cacgtagggg cggtcgaaca agggggcgag ctggtcttcc tatacagact ggcgcggggg
     2761 agtgtagacc gaagctacgg cctgaacgtg gcgcgaatgg cggggctgcc cagggaggta
     2821 atcaggcggg cgcagcagat cctgcgaggt ctggagagcg ctcagccgtc gggcgggcat
     2881 caaatgacct tgttcggctg ggcggacgag gaacctctgc cgatagccac tgctgaggct
     2941 cgcagcgacg gaggcgtcgg cgggcgggtg atggagctgc tcgatagcat tgaccccgat
     3001 agcctaactc cgcgcgaggc cctgtcgttg ctgtacgagc tcaagagaat gcaggagtag
     3061 gtgatcccat atgcgcatcc tgaaacttgc gccagaagtc gccgatagga tcgcggcggg
     3121 agaggtcgtc gagcgcccgt cctctgttgt gaaggaactc gttgagaact cgatagacgc
     3181 aggcgcacgg gccgtacgaa tagagatcgc cggcggaggg ctcgagatga tctccgtatc
     3241 tgacgatggg tgcgggatga gccgcgaaga tgcttcgctg gccttcgagc gccatgcgac
     3301 gagcaagata tctcgggcag aggacttgtt ttccattgtc acgctaggct ttcgaggcga
     3361 ggcgctgccc agtgtggcgt cggtggcccg agtgaggctg gtaacgcggg agcgtggcgc
     3421 ggctcacggc acggtggtga ctgccgaagg cagcgttgtg agcggaccgt ctgccgtagg
     3481 cgcgccggag ggcactactg taaccgttcg ggacctgttc tataacacgc cagcgcgtct
     3541 gaagtacctt cgctcagtgg ccactgagac ccgccgtgtc atcgagaccg tcggcaggct
     3601 ggcccttgcc catccggagg tctccttcag ccttgacgtg gatggccgaa acgtgctgtc
     3661 taccccgcag ggcggatcgt tacgcgaggc cgctgcagct gtactcggca ttgagctcgc
     3721 gcgctccatg gcgccggtgg acaagacgac tcacgggata acagtccggg gcttagcagg
     3781 accgggcgac tgcgccaagt ccagccggga cgtgcagcat gttctggtca atggcaggcc
     3841 ggcatcgtgc aagacggtat gggctgccat agaccgtgca tacgataggt ccacgccgcc
     3901 cgggaagaag gcccctgtgg tgctctcgat caccgtggac ccatcatctg tagacgtcaa
     3961 tgtgcaccct gccaagagcg aagtcaggtt tgcgtcggag tcggatgtgt actcagcagt
     4021 atatggcgcg gtctccgccg ctctgcgctc tatggatgcg gtgccggaca tcggctcgaa
     4081 gatgcgcgat ggcgagcagc ccgggcgtgg cgtcgagctg gacgacgacg catctagacg
     4141 cggtagcgcc gccggcgccc ggctgtggcc gagcattgcc gccgataggc gagaaggccc
     4201 ggcttacgga ggcggccggc aggccgaata cgatacgagc tacggcaaga acgccgacta
     4261 tggcacgggc gcaggcaacg ttgcttgcga gcgcagcgtt ccggttacca taatcgggca
     4321 gtatgcagac gcctatattc tggctgagag cgatgagggt atgctcatta tcgatcagca
     4381 tgtggcccac gaaacggtgc tggtggacac gtacaggcgg cagcttgcgt cggcgtcatc
     4441 gggacccgtc acgcagctat tgctctcgcc cgaacttgtg cagcttggag ctgccgatac
     4501 ggatcttgtg gcgcgccacc agtccacgct ggcgaggatg ggttttgagg cggacgtatt
     4561 cggcgtagca tcagtcctgg tgcgaggcat cccgatcgca gcagcggaca tgacgcctgg
     4621 agaggcgctg ggcgcagcta tcgagggaat cgcggcagcg tccgcggagg cagatcctga
     4681 gtctttagcc gaccatatcg ccgtgtcgct tgcatgccac agcgccgtga aagccggcgc
     4741 ccacctacac gaagaggaaa tgaagtcgct ggtagcagcc ttgttccggt gcgagaatcc
     4801 catgaggtgc ccgcatgggc ggcccgttat cgttacggtc tcgaaggagt ctctcgaaag
     4861 gagcttcggt cggcgatgat catcacacgt gggctggtag tcactacgtc atcgcgcgca
     4921 ggagcctccg aaatcgagcg tgccagaaag gttgcctcag ctctgaactc gccctacatc
     4981 gagcgacaca gcaagtcggt tgcccaggtt gcgaaggctg ccgaggttgc ccgcgaatgg
     5041 ccgggtccgg gtaaggtcga aggcgtgctc gttgtcgagt cgagcaggat gacgctggtg
     5101 ggcatgggcc agtccggccc cgccggcccc cgtcttttct tccatcccgg aatggggccg
     5161 ggcaggatca ggcgcgcggc gcagggcagg caagatcaca tgctggaggc catgggtctg
     5221 ggccggggcg accgcgtgct cgattgcacg ctgggcctag caagcgacgc gatcgtggcc
     5281 gcttgggctg tcggctcttc agggaaggtg gtcggcatcg agtccagccc ggcgctggcc
     5341 gagatcgtgc gggccgggct gcgcgagtac gacaaggcag acccagacac gactgatgcc
     5401 atgcgccgaa tagaggtggt ctgcgctgac catttgatgc acctgatgtc gtgtccggat
     5461 cagagctacg atgtagtata cttcgatccc atgttccgtg agcctgtgcg cctttcgccc
     5521 ggaattgccg gattgcgccg atgggcatgc cgttccaggc tgtcggggaa cgctgtgcgt
     5581 gaggcgacgc gggtggcacg gcgccgagtg gtcatcaaag acaggagcga aggctctgag
     5641 ctagataggc tgggcgtaac tatgcgcctg ggcgggaggg gaagccgggt ggaatatggc
     5701 gtcatatgcc tcactgatga ggtgacgtgc cgatgacttg gccgatgcgc attcccttgc
     5761 tggtcatcgt gggccccacg gcatctggta agtccgccgt agggatggcc ctggcgcgca
     5821 gactggacgg ggagatcgtg tcggcggact ccatgcaagt ctacagggga atggacatcg
     5881 gcaccgccaa accgaccccg agcgagcagg ctgagatccg ccatcacctc atcgatgtag
     5941 ccgatccgga cgaggtgttc aatgttgcgc gttaccagga gctggcttcc gccgccatcg
     6001 atgatgcggc tgtccggggt aagttgccga taatggtggg gggcacaggg ctgtatgtgg
     6061 atgctgtcgt caggggattt ctttttccgg atgagggacg caattcggca gttcgggcca
     6121 ggctagagca agaggcggca ggtgagggcg gcgctccgac gctccacgcc aggctggcgt
     6181 cgtgcgatcc cgaagccgcg cgaagaatac accctaacga tctgcgacgg atcgtgcgcg
     6241 cccttgaagt attcgaagtg accggccgtc ccatcacgga gctccggagg aagcacgtgc
     6301 ccgttaacgc gtacagtgcg aggcttttcg gcctgacgat gccgcgcgag gcgctgaccg
     6361 agcgtattga tgctcgcgtg gaccaaatgg tcgcagccgg gctggttgac gaggtgcgca
     6421 ggctgcttca gatgggctat gacggtcaat ccacggctat gcaggcaata gggtacaagg
     6481 agtttgctgc gtatctggcg gggcgtgagg ggctagagca ggcaattgat gaggtgaagc
     6541 tggagacgcg caagtacgct aagcgccaga tgacatggtt ccgaaagcac gacgacatca
     6601 cgtggattga tgttcaggtt gcctcggacc cttcggacgt ggcttccgag atcatggcac
     6661 ggcttggcga ctggctggag gaggcagaca cagcacttgg acgaacgcag ggacgacatt
     6721 aatgacaatg acgacacgaa caggaacgac gttacgaacg gcaactacgt tatgaacggc
     6781 aactacgaca cgagtaacct ggacgaccgg gagatcgcgc gcgctctggg cctgaacgat
     6841 gagtggcttg atgccgcagg ccggatcagg gcgaggatcg cgcctgaggt tgctgtgacc
     6901 gacaagatcg caagggtaaa cagcgcgcga gtgctggcag cgctgggtcg ggcaggcctc
     6961 ggagactatc atttcggcgg caccagtggc tacgggtatg acgatacggg cagggagcac
     7021 ctcgaaaggg cattcgcgga cgtgtttggc gcagaggcag ccctggtgcg aatacagatg
     7081 gtgtctggca ctcacgccct ggccgccgtg ctcttcggcg tgttgcgccc tggggacctg
     7141 ctggtgtcgg cgtatgggtc gccttatgac accatggtca ccatgatagg ccatgatcga
     7201 cgtgtgcgcg gttcactggc cgaacatggg gtggagtatc tggaagtacc cgcgggcgag
     7261 ggcggagagc cggactacgc ggggatatcg cgggccgcag aaatggcccg aatggtgctg
     7321 attcagcgtt cgcgcggcta ctcctggcgt agagctctgt cgtgcgagaa tattggcaga
     7381 attgctcgct gcgtaaaggc ggccaatccg cgggcagtcg tgttcgtcga taactgctac
     7441 ggcgagttca ccgagacaat ggaaccctgt gcggttggag cggacgtggt ggccggatcg
     7501 ctgatcaaga accctggggg cgggatagcc ccatgcggtg gatacgt
//
LOCUS       DAOTAC010000103         7464 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_8408_length_7464_cov_4.854366, whole genome shotgun sequence.
ACCESSION   DAOTAC010000103 DAOTAC010000000
VERSION     DAOTAC010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7464
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8408_length_7464_cov_4.854366"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            361..1596
                     /locus_tag="PLM74_05710"
     CDS             361..1596
                     /locus_tag="PLM74_05710"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HON42615.1"
                     /translation="MAGALDQLIHTARTPVEQCGKGVVMHTPQEIRRQAELWPDTAQL
                     MARNLPEILDQLCHMRHEDASDGAMAARSIVLSGAGSSHHVGMSLEPFLRKRLQVEAR
                     AVASTDIVLDPSVTCLKDHPYLVVLFARSGDSPESLGAIDAVRSVCPNARFLAITCNE
                     HGGLATAAREGAFDGAVTAVVLDERTNDKGLAMTSSFTNMVVAGQALGLVEDMDEYLS
                     TVDLLAAAGERAVTKLADAVKDVADRSFARALFLGSGALYGAARESALKLQELTDGAV
                     VTQSETFLGVRHGPEAVVNEETLVVYFVSGDEYRRRYELDLINEVHAKQPRALKLAVA
                     PRASRDLAGIVDVCVELAPTGPFVHDDLRPPVDAVIGQLIALFKSVSLGMEPDNPSRR
                     GMISRVVEGVTYYPLSGNR"
     gene            1634..2605
                     /locus_tag="PLM74_05715"
     CDS             1634..2605
                     /locus_tag="PLM74_05715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HON42616.1"
                     /translation="MSLQNMTTLLSFVRDGQAIAAFNFIDLDDLRGIVCAAEHEQTPV
                     ILQVSQSTAKHLGLDYAAGMARVAARRSRVPLCLHLDHASGIDIICEAIECGFTSVMY
                     DGSALPFEDNLDFTARVVEMAHAAGVSVEAELGIVGGKEEAMNEKTGEETAGEITGGM
                     DEGIAREIAREIAEEMTGDPGKERSVAIHGHPLTDPLQSREFVLRTGIDALAPAVGTA
                     HGLYRGAPHIAFDLISAIRDLVNVPLVLHGGSDIPEELLRQAIRAGIRKVNVGTDLQV
                     AHAQALRRALPGEGEVLNMRATHAAAVDAVCAVARAKIAICSGRGAS"
     gene            2880..4037
                     /locus_tag="PLM74_05720"
     CDS             2880..4037
                     /locus_tag="PLM74_05720"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HON42617.1"
                     /translation="MMGSKMIGPKTTGYESMYGRAACAVMAILLVFFALGGAGTECAT
                     PGSDAGSIVSGSQSVKVGTKAFQVGYVRVDLSDPTIRLRVVLGQDRVGGTEDLLSMAA
                     RVGAQAAINGTFFNAYVDGPYKDPVGTLIQGGEFVHRGATGTVFAVAGGNELRMEPAR
                     FKIVGATNGLWTWPGNWYSYWINRTPTSQNYAGIFTPARGSRTGAADGVSVVVRDGTV
                     TAIGRGEHEIPQDGYVINFQGSEEGLAARFAVGAAVEYKVVMSDGSDRTGFWGRVSEG
                     LGAGPKLVSGGKVSYSAESAKAEGFTEAKILSQSSARSGLALTKDRNLLMVTCAAATV
                     AQFAQVMHAMGAVEAMNLDGGASSGLVCGGRYLTKPGRALSNGLVVLADGR"
     gene            complement(4041..5585)
                     /locus_tag="PLM74_05725"
     CDS             complement(4041..5585)
                     /locus_tag="PLM74_05725"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HON42618.1"
                     /translation="MGHVIVGALVISGIVQLSIGIYVMVQSKWGKNLWFSCLTIAASI
                     YSFGYAFELTAQTVSEALVYLRVEYLGIAFLPTLGLLTALEFTGKRWNPSSKPAIAML
                     AFSILTLVGMYTTHLHHLYYADLVLTRVGVLTIVQITRGPWYMVSMTYANLATVAGVA
                     VVARSIITAPADLKAGQWLLLVGFALMGLSSNAYAAGLSPYGVDLTPYGFMAVNVLLL
                     IGLSPGQVFDLVSIAKSRVFDGMYDIVVIIDRNNRVADYNPALHQIVPDRGNDYAGEL
                     LPVVFEKHPEIAEFAAAHRDGETDIALNDGTGRVFRVHIRTVGQNSRRNGRRMLAKYL
                     TMTDITKEMAMVRTMKNLADLDALTGIANRRSFYDQVEQLTGRQRVGCPVSIIMLDID
                     HFKAVNDTYGHRAGDMVLQGIASLLSQNTRAQDILARYGGEEFIIAVPGMGTEDAMAL
                     AERLRKSINAFEGVYDGQLIKVTASFGIACDTAGPGFNMEFLIRNADTALYEAKGLGR
                     DTVVRR"
     gene            complement(5700..6632)
                     /locus_tag="PLM74_05730"
     CDS             complement(5700..6632)
                     /locus_tag="PLM74_05730"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HON42619.1"
                     /translation="MITLNALVTFDLDGVLMQNPFRRGVFPHVVRALEPYASSIAARE
                     TGTDPKRLVLQLIIDEARQRLQAGRMVEAYDWDDIVGRVAEDLACRAGALEAQSVALS
                     GAPSGVPPVTHPDVLPEIPHFDIAALVRHYCTVPGMIARLEGAYETLDALKGAGYTLA
                     VVTNGYLKYQAPVLDALGLIDFFSAITTPEQAGAAKPMPGIFRTAWAGWREGRNMPIH
                     VGDDIIHDAWGAKQAGGYSVWLRPDIPHSLRGLLPEDRPFATDFASVRDASFRATMAA
                     DAFGASPGDCQPDALICTLGELPALLHRLKFRDM"
     gene            6810..>7464
                     /locus_tag="PLM74_05735"
     CDS             6810..>7464
                     /locus_tag="PLM74_05735"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HON42620.1"
                     /translation="MSRRVLIAIGIVLMIALGSHMPAAMAAAPSKPITITWWINPWRI
                     APPGFPDDKAPTAEDFPKWASEEFMRLNPGVTVKYEVVTNTGFDQKIAAAILAGNPPD
                     VLRPIGFKREWARQGLLEPIDDYLTAEERQDFYQYALDVGKVSGRYYLFPWNNSNNGM
                     GSTLLINPAVFKERGVSTPSLPERGWTVDEFMQAAQRLTFDRDGDGKTDVYALSFPAK
                     "
BASE COUNT         1514 a         2022 c         2386 g         1542 t
ORIGIN      
        1 gcgcctatcg gcaaaactat cggcaaaatt gacggcatgc ctactcggat acccccgggg
       61 gtataccgtt gagctgtcgt gatcgccttt ttgtacccgg atctgtccac ggatgcatcc
      121 tcgggccctt tctaccccag tctctttcga ggtggtcccg gggaggtgct cccggcgagg
      181 ccccctgcca gaagccaaat ccggagtcgg ctgggtcgag cggggtgcca actggcggat
      241 tggtgctagg ccgaagcggg agatatccga ggttctaata gagacaatcc ttacgcgcac
      301 ctcaggggtt gctcagggtt gtgtgctgtt tgcagcagat gaagtgggag ggaatcagtg
      361 atggcaggag cgcttgacca gctcatacat accgcgcgga cgcccgtcga gcagtgcggc
      421 aagggcgtcg ttatgcatac gccgcaggaa atccgcaggc aggcggagct gtggcccgat
      481 actgcgcagc tcatggctcg gaatctgcct gagatactcg atcaattatg tcacatgcgc
      541 catgaggacg cctccgacgg agcgatggcc gcgcgtagca tcgtgctgtc gggcgccggc
      601 agttcgcatc acgtaggaat gtcgctggaa ccgttcctca ggaaacgcct tcaggtggaa
      661 gcgcgcgccg tcgcctcgac tgacatagtg ctggacccgt ctgttacctg cctcaaggat
      721 cacccttact tggtagtact gttcgctcga tcaggcgata gccccgagag cctgggcgct
      781 atcgatgcag ttcgctcggt gtgcccgaat gccaggttcc tggcgattac ctgcaacgag
      841 catgggggtc tggcgactgc ggcgagagag ggagcgttcg acggggctgt gactgctgtg
      901 gtcctagatg agcgaaccaa tgataagggc ctggcgatga cctcgtcttt cacaaacatg
      961 gtcgtagctg gccaggcttt ggggcttgtc gaggatatgg acgagtacct atcgactgta
     1021 gatttgctgg cagcggcggg cgaacgagct gtcacgaagc ttgccgacgc ggtgaaggac
     1081 gtcgctgatc ggtccttcgc gcgcgccctc tttttgggca gcggagcgct gtacggggcc
     1141 gcgcgcgagt ctgcgctcaa gctgcaggag ctgacggatg gcgccgtagt gacgcagagt
     1201 gagaccttcc taggggtgcg gcatggcccc gaggcagtag taaatgaaga gacactagtc
     1261 gtctatttcg tttcgggcga cgagtacaga agaagatacg aactcgacct gatcaacgag
     1321 gttcacgcca agcaaccgcg agctctcaaa cttgcggttg cccccagggc atcgcgggac
     1381 ttggcaggca ttgtggatgt gtgcgttgaa ctggcgccga ccggcccatt cgtccatgat
     1441 gacctgcgcc cgcctgtgga tgcggtgata gggcagttga tagcgttgtt caagtcggta
     1501 agcttgggaa tggagcctga caacccatcg aggcgcggga tgatcagcag agttgtagag
     1561 ggggtgacgt actatccgct gagcggcaac cggtaagatg attgaccgtg aggggaaacc
     1621 gggaggcatt gctgtgagtc ttcaaaacat gacgacgtta ctaagcttcg ttcgggacgg
     1681 tcaagccata gcggcgttca acttcatcga cctggacgac ttgaggggaa tcgtctgcgc
     1741 ggcagagcat gaacaaacgc ctgtcattct ccaggtgagc cagagcactg ctaagcactt
     1801 ggggctcgac tacgcggcgg gcatggcgcg ggtggcggcc cggcggtcgc gagtgccgct
     1861 atgcctacac ctagatcatg cgtccggcat tgacatcatc tgcgaggcta ttgagtgcgg
     1921 ttttacttca gtgatgtacg acggatccgc gctgcccttc gaagacaacc tggatttcac
     1981 cgctagagta gttgagatgg cgcatgctgc cggggtaagt gtagaggctg agctgggcat
     2041 agtgggcggg aaggaggagg cgatgaacga gaaaacgggt gaggagacgg ctggggagat
     2101 aactgggggg atggatgagg ggatagctcg ggagatagct cgggagatag ctgaggaaat
     2161 gaccggggac cccggcaagg aaagaagcgt agccatccac ggccacccgc tcactgatcc
     2221 gctgcagtcc agagaattcg tgctgcggac gggcatcgat gcgctggcgc ctgctgtcgg
     2281 tactgcccac gggttgtacc gaggagcccc acacatcgct ttcgacctga tatctgccat
     2341 tcgcgacttg gtgaatgtgc ctcttgtcct tcatggaggg tccgatatcc cagaggaact
     2401 gcttcggcag gccattcgag ctggaattcg aaaggtgaat gtagggactg acctgcaggt
     2461 ggcgcatgcc caagcgttgc gccgggcctt gccgggcgag ggcgaggttc tgaacatgcg
     2521 tgcgactcat gcagcagccg ttgatgcagt gtgtgccgta gcccgcgcca agatcgcgat
     2581 ctgttccggg cgcggggcgt cgtagcacac gggaccggcg ctttacgctg gccctggggc
     2641 gcaaatgccg gttgagctgg ccttgttatg atctgttcgt tttgtgaaca cattttgcga
     2701 gatgtgtcgc tgtgaccgag gtcgaatcac attcgtatcg tctgcgtggg ccgcacgtca
     2761 agctgtctat ggggacggca gcatgcatga gcggtagtct gcatgggcgg gcatgcagac
     2821 atgcgagtgc cgcgagtgct gcgagcagcg aattgcgggg atttggaggt atgtgcagga
     2881 tgatggggtc caagatgata gggccaaaga cgacgggata cgaatcgatg tatgggcgcg
     2941 cggcctgtgc agtcatggcg atactgctgg tgtttttcgc gcttggcggg gccggaacag
     3001 agtgcgcaac cccgggctct gatgccggca gcattgtatc cggatcccag agtgtgaagg
     3061 ttgggactaa ggctttccag gtgggctacg tccgggtgga tctgagtgat cccacgatcc
     3121 gtcttcgtgt agttctgggg caggataggg tgggcgggac tgaagacctt ctatccatgg
     3181 cagcacgagt gggcgcgcag gctgcgatca acgggacttt ctttaacgcc tacgtcgatg
     3241 ggccctacaa ggatcctgtc ggaacgttga tccagggcgg cgagttcgtt caccggggag
     3301 ccaccggaac ggtgttcgct gttgcgggcg gaaacgaact gaggatggaa cctgcacggt
     3361 ttaaaattgt gggcgcaacc aacggcctat ggacgtggcc ggggaactgg tattcgtact
     3421 ggatcaacag aacgccgaca tcgcagaatt acgcggggat tttcacgccg gcgcgaggaa
     3481 gtcggaccgg agcggccgac ggagtatcgg tggtggttag ggatggcact gtcaccgcga
     3541 ttggccgtgg cgaacacgag atccctcagg acggatacgt catcaacttc caaggaagtg
     3601 aagaaggact cgctgcccgc ttcgcagttg gtgccgctgt cgagtacaag gttgtgatgt
     3661 cggacggcag cgacaggaca ggattctggg ggcgagtcag cgaaggcctt ggggcaggcc
     3721 ctaagctagt gtcaggggga aaggtaagtt attccgcgga atcggccaag gcggagggat
     3781 tcaccgaggc caagatcctc tcgcaatcat cggcgcgaag cgggcttgcg ctgacgaaag
     3841 accgaaacct cttgatggtt acctgcgccg cagcgacggt ggctcaattt gcccaggtaa
     3901 tgcacgccat gggggcggtc gaggcaatga accttgacgg gggagcatcc tcaggattgg
     3961 tgtgcggagg aagatacctg accaagcccg gacgcgcgct cagcaatggc ctggttgtgc
     4021 tggccgacgg gcggtgatga tcagcgcctg acgactgtgt ctcggcccaa gcccttggcc
     4081 tcgtagagcg cggtgtctgc gttcctgatg aggaattcca tgttaaaacc tgggcccgct
     4141 gtatcacatg cgatgccgaa gctggctgtg accttgatca gctgcccgtc gtagactccc
     4201 tcgaaggcgt tgatgctctt gcgaagcctc tctgccaacg ccatagcgtc ctctgtgccc
     4261 atgcctggaa cggctatgat gaactcctcg cctccgtatc gtgcgagaat gtcctgggcg
     4321 cgtgtgttct gactaagcaa tgatgcgatt ccctgaagca ccatgtcgcc ggctctgtga
     4381 ccataggtat cgttcacggc tttgaaatga tcgatatcca gcatgattat agacacgggg
     4441 caaccgactc gctgcctgcc ggtcaactgc tctacctgat catagaacga cctgcgatta
     4501 gcgatgccgg tcaatgcgtc gagatctgca aggttcttca tggttcgaac catggccatc
     4561 tccttggtga tgtcggtcat ggtgaggtac ttggccagca tgcgtctacc attgcgcctg
     4621 ctattctggc ctacggtgcg aatgtgcacc ctgaacacgc ggcccgtacc gtcgttcagc
     4681 gcaatatcgg tttccccatc ccgatgcgct gcggcgaatt ccgcaatctc cgggtgcttt
     4741 tcgaagacta cagggagaag ctcgcctgca tagtcgttgc ctcgatccgg tacgatctgg
     4801 tgtagggccg ggttgtagtc ggcaacccgg ttgttcctat cgatgatgac gacaatgtcg
     4861 tacatgccgt cgaagacccg actcttggct atcgacacaa gatcaaacac ctgaccagga
     4921 ctcagcccta tgagcagaag cacgttaact gccatgaacc cgtatggtgt aaggtccacg
     4981 ccataggggc taagccctgc cgcatatgcg ttgctactta gtcccatcaa ggcgaaaccc
     5041 accagtaaaa gccattgccc agccttgagg tcagccggag cagtgattat tgatcgggcc
     5101 actacggcaa cgccggcaac cgtagccaga ttcgcatatg tcatactcac catgtaccaa
     5161 gggccgcgcg taatctggac tatggtcagt actcccactc tggtgagcac cagatcggca
     5221 tagtacaggt ggtggaggtg ggtggtgtac atgccgacta gggtgagtat ggagaaggcg
     5281 agcatggcga ttgccggttt gctgctcgga ttccagcgct tccctgtgaa ctcaagggca
     5341 gtgagtaacc cgagtgtggg caggaaagct atgcccaggt attcgactct gagatagacg
     5401 agggcttcgc ttactgtctg agcagtgagt tcgaaggcgt agccaaacga gtatatagat
     5461 gcagcaatgg tgagacagga gaaccacagg ttcttgcccc atttgctctg aaccatcacg
     5521 tagatgccga tgctcaactg gacgattcca gatatcacca gggcacctac gatcacatgc
     5581 cccatactaa catcctgcct agctctgtga attccgtgcg ccacccgcga ctagttacac
     5641 atttcgcgtc aattatcgta tttcctctat aagtgtcgat taccgaagtg cagccgcgct
     5701 tacatgtcgc ggaacttgag ccgatgcagg agcgctggca gttcgcccag ggtgcatatg
     5761 agtgcgtctg gctggcagtc gccgggcgac gcgccgaatg catctgccgc catggtggct
     5821 ctgaatgaag cgtcccggac cgaggcgaag tccgtggcga acggcctgtc ttcagggagc
     5881 aggccgcgca ggctgtgcgg tatgtctggg cggagccaca cagagtagcc gcccgcctgc
     5941 ttcgcgcccc aggcgtcgtg gatgatatca tctcctacgt gtatgggcat gttccgtcct
     6001 tctcgccatc cggcccacgc cgtgcggaat atgccgggca tgggcttggc tgcgccggcc
     6061 tgctcgggtg tggtgattgc gctgaagaag tcgattagcc ccagggcatc cagcacgggc
     6121 gcctggtact tcaggtaacc gttggtgacg acggcgagtg tgtagcctgc gcctttgagt
     6181 gcgtctagcg tttcatatgc accttcgagg cgcgctatca tgcccggcac tgtgcagtag
     6241 tggcgcacca gggcggcgat gtcgaagtgg gggatttcgg gcagcacgtc ggggtgtgtg
     6301 acgggcggta caccggaggg cgctccggag agtgccacgc tctgcgcttc tagggcgcca
     6361 gcccgacagg ccaagtcctc ggcgacgcgc cccacaatgt cgtcccagtc ataagcctct
     6421 accatgcgtc ctgcctgcaa cctctgtctt gcttcgtcaa tgatgagctg aagaaccaat
     6481 ctcttcgggt ccgtgccagt ctcccgggca gctatggacg aggcgtacgg ctccagggcc
     6541 cggaccacgt gtggaaacac gccccgtcgg aatgggttct gcatgagtac tccgtctaga
     6601 tcgaaggtta ctagagcatt cagagttatc acctctgata gaaggaacta gagccctacc
     6661 gccgaaatac cagtggacga ggctgcgcgg ggctgtgcct gcaacagcga ataacgcgct
     6721 gcatccctgg aatgtgattc gcgctacggg tctctagtcc tgcctaccat cggcccggtc
     6781 acagaatacg cctaaaggag gggtcacgag tgtccagacg tgtgctcatt gccatcggca
     6841 ttgtcctcat gattgccctt ggaagccata tgcctgcggc tatggctgct gctccgtcca
     6901 aacctatcac catcacttgg tggattaacc cctggaggat tgcgccgccc ggtttccctg
     6961 acgacaaggc gccaacggcc gaggactttc caaaatgggc aagcgaggag tttatgaggc
     7021 ttaaccccgg cgtgacggtc aagtatgaag tcgtcactaa caccggattc gaccagaaga
     7081 tcgccgcggc gattctcgca ggaaacccac ccgatgtact gcgacccatc ggcttcaaac
     7141 gtgaatgggc caggcaggga ctgctagagc cgatcgacga ctaccttaca gcagaggaaa
     7201 ggcaggactt ctaccagtac gctctggacg taggtaaggt cagcggtcgc tactacttgt
     7261 tcccgtggaa taacagcaat aatggcatgg gttcgacgct cctgatcaac ccggccgttt
     7321 tcaaagagcg cggcgtgagc actccgtcac tgcctgagcg gggctggact gtcgatgagt
     7381 ttatgcaggc cgcccagagg cttacctttg accgcgacgg cgacggcaag actgatgtgt
     7441 acgcgctcag cttccccgcc aagg
//
LOCUS       DAOTAC010000104         7456 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_8420_length_7456_cov_4.375220, whole genome shotgun sequence.
ACCESSION   DAOTAC010000104 DAOTAC010000000
VERSION     DAOTAC010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7456
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8420_length_7456_cov_4.375220"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..853)
                     /locus_tag="PLM74_05740"
     CDS             complement(<1..853)
                     /locus_tag="PLM74_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017436550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HON42621.1"
                     /translation="MKEALLEVTDLKKHYPVTGGLFRRTVGTVRAVDGISFDVEKGST
                     FGLAGESGCGKSTAARTILRLEEPTSGSIRFNGEDFSAKGPEELRLARTDMQMIFQDP
                     YSSLNPRHTVAKIISEPLRVHNIGTHAEQKERVRHLLDVVGLPTDAAERYPYEFSGGQ
                     RQRVGIARALAMRPKLIIADEPVSSLDVSIRAQILNLLRKLQDQFGLTYIFIAHDLAL
                     VKNMSPQIGIMYLGHLVETGPSGQVFGSPAHPYTQALIAAVPGAGIWRRDKDRTTAKP
                     AAPLTGDV"
     gene            complement(850..1830)
                     /locus_tag="PLM74_05745"
     CDS             complement(850..1830)
                     /locus_tag="PLM74_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020373911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON42622.1"
                     /translation="MDEPTLVVEDLVTWYLTERGPIHAVDGVSLAVQRGETLALVGES
                     GCGKSATALSIMRLIPSPPGLIVSGRVLLEGRNLLELSEPDMCKVRGGDISMIFQEPM
                     TSLNPVYTVGFQVAEAILVHHKVSEREADKEAVELLDRVGIASPARRAKDYPHQMSGG
                     MRQRVMIAMALAANPKVLIADEPTTALDVTIQSQILDLIRQLQQELNMGVLLITHDFG
                     IVAEVADRVAVMYAGQIVEEGSTGALLECPTHPYTRGLISSVPKMDDPTGTMLYSIEG
                     ASPNLLDPPTGCRFAPRCGEARTECRECRPELESTGDGRRVRCLGGGDRR"
     gene            complement(1823..2761)
                     /locus_tag="PLM74_05750"
     CDS             complement(1823..2761)
                     /locus_tag="PLM74_05750"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HON42623.1"
                     /translation="MHDIASRLWRHPRGRVGIIITAAVIIIAIAAPWIAPYDPYDIGQ
                     RGPRRLPPGPGHPLGTDAQGKDILSKLIYGARVSLIVGITTGVCVTVLGALLGIVAGY
                     LGGFADTLIMRTCDILFVVPGLPLMILLANYMGTKFYMIIVIFTMLGWAGVARMIRSQ
                     VLSLKSRDFVVASRSVGARPGHIMREHILVSVSPLLIVNGVLSAAGVMVAEAGLSFLG
                     FGDPTAVSWGKMLYEAQSGHALLFGAWWWVIPPGLAIFITVFGFMMFGYALEEVLNPH
                     IPRQSAYSKLRKRMARYAGIGKQAGIGRPGGGESIG"
     gene            complement(2774..3745)
                     /locus_tag="PLM74_05755"
     CDS             complement(2774..3745)
                     /locus_tag="PLM74_05755"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HON42624.1"
                     /translation="MSGAYWARRLLQTALILFIVVTLNFFLPRLTPGTPAGHLYNDPR
                     LSPEAKQEILAQFGLDKPLHVQYAVYLGNLLRGELGTSFSYRRPVSEIILSRIPWTLV
                     LTLSSFLVSVALGVALGAAAAWKRGGHLDTILLSASIVVSALPSFWIAMILTLILAFY
                     WPVFPAYGMVSPHVPVGWNWAFIGSVARHAFLPVLSMCIGGIVGYGILVRNSMVEISG
                     EDFVRTARAKGLSENTALYRHALRNAMLPLVTRIGMSLSGLVGGATVIETIFSWEGMG
                     LLIVEASRSLDYPLMQGTMLIMAVITILANLCADLAYARLDPRVKLK"
     gene            complement(3748..5379)
                     /locus_tag="PLM74_05760"
     CDS             complement(3748..5379)
                     /locus_tag="PLM74_05760"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HON42625.1"
                     /translation="MEKPLRSVVIAILCVGLLMVPTGAGVAGAAKAVGNLNLGVIADV
                     KNLNPYMGYGSSETFVIGWVYDTLLGWDSDEGLVPGLADSWEIKDGGTSVEFKLNPNA
                     KWHDGKPVTAEDVEFSFNFIREKRFPSFMAIIGNLAGAKAIDKHTVRVTFRSPSSNAV
                     RFIATAPSILPKHIWSKIDDPRNYPNLDNPIGCGPCAVAERRDGQFITLKNLGNHYRS
                     SLNVETITLRVLRDETMGIMALRRGDLDALLWSVDPAVAEEIDRNPKGYPNVKVAMAP
                     STSVRTLMFNLRRAPWNDATLRKAVSLAINQKELIDQVLLGYGSEQGPGLVVPSDLHY
                     NKALGPVPRDVAKAKAILDQAGYVDKNRDGIREDKNGKPIKLEILTGNLPTSINLAEL
                     LVYQIKQIGIDAYFTPLTAESLRNKEQAADFDAAVSSVSFSVPDMMFYYAHTSRGVIN
                     EGRVAGFNYGGYSNPEYDKLAEAMLVEMNDQARIKLLRDMQAKLAEAYYHIPLFSSNV
                     LMLYRDDRFTGWTVEPDSCINNSTTYSNLVYKGGK"
     assembly_gap    5545..5644
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5645..6203)
                     /locus_tag="PLM74_05765"
     CDS             complement(<5645..6203)
                     /locus_tag="PLM74_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42626.1"
                     /translation="MDEFRLPDKFVRWAYDGRAELVRRQAQGDQVSRDEVFLGFTRHT
                     PTFISNGSAGLNGSIKGVGFIPKAEYLDEVLDAYLEHIGRGWRDGYSEEGLQLLVKYM
                     WGEGCKERIDFSILGSIELALKHSWTNYLENPEVTLVYYQPPAITYEVRGKMEIHRDG
                     KYHKFINAQHDVYHKPNTDLWQKRPV"
     gene            6473..6904
                     /locus_tag="PLM74_05770"
     CDS             6473..6904
                     /locus_tag="PLM74_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42627.1"
                     /translation="MTNSADAHLDRAILCVDSYIYFGTDDHFVNYILKFLAPGGTLAV
                     AMPGLMKDSEDGVSHHLKPFWGQDCWCWHRLEWWRHLWERTGLVDMVEADTLPDGCAS
                     YVRWREAQDKAGLNPWPQDIGILKQDAGEYVGFIRLAARKT"
BASE COUNT         1516 a         2230 c         2026 g         1584 t
ORIGIN      
        1 gcacatctcc tgtgagcggc gctgccggtt tcgcggtggt gcggtctttg tcgcgtctcc
       61 atatgcccgc gccgggcaca gcagctatga gcgcctgggt ataggggtgt gccgggctgc
      121 caaacacctg tccggacggc cctgtctcaa ctaggtgccc caggtacatg atgccaatct
      181 gcggcgacat gttcttgacc aacgccaggt catgggcgat gaatatgtag gtcaggccga
      241 actggtcctg gagtttgcga agcagattga gtatctgggc ccggatcgac acatcaagcg
      301 atgacaccgg ctcatcggct atgatcagct tgggacgcat agccagcgcc cttgcgatcc
      361 caaccctctg acgctgcccg ccgctgaact catacgggta tctttcggcg gcatccgtag
      421 gcagtcccac gacatcaagt agatgcctga ctcgttcctt ctgctctgcg tgggttccga
      481 tgttatgtac cctgagaggc tccgagatga tcttggcgac agtgtgacgc gggttgagcg
      541 atgaatatgg gtcctggaat atcatctgca tgtcggtgcg ggccaggcgc aattcttcag
      601 gtcctttagc ggagaagtcc tcgccgttga acctgatact gcctgaagta ggctcttcca
      661 gccgaagtat ggtccgggcc gccgtggact ttccgcatcc gctctcgcct gcaagcccga
      721 acgtagagcc tttttcaaca tcgaaagaga tcccgtccac cgcccgcaca gtccccacag
      781 tccgccggaa taggccgccg gtaaccgggt agtgcttctt caggtctgtt acttcaagca
      841 aagcttcctt catcgcctgt cacctccgcc cagacatctc accctgcgcc cgtctccagt
      901 gctctccagt tcaggccggc actcacggca ctctgtgcgc gcctcgccgc atctgggagc
      961 aaaccggcat ccggtcggcg ggtccagcag gttaggcgac gctccttcaa tcgagtagag
     1021 catcgttccc gtgggatcgt ccatttttgg cacggacgag ataagcccgc gcgtgtatgg
     1081 gtgcgttggg cattccagca gcgcgccggt cgaaccttct tcgactatct gccctgcgta
     1141 catcaccgcc accctgtccg caacctcggc cactatcccg aaatcatgcg tgatgaggag
     1201 cactcccatg ttcagctcct gctgcagctg acggattagg tcgagtattt gcgactgaat
     1261 tgtcacatcc agggcggtgg tgggctcgtc cgcgatcagg accttagggt tggccgcgag
     1321 agccatggcg atcatgaccc gctgcctcat cccgccgctc atctggtgcg ggtagtcctt
     1381 tgcccgccga gcaggggatg caatgcccac cctgtccagc agttccacag cttccttgtc
     1441 tgcctcgcgc tccgagacct tatggtgaac gaggatggcc tccgccactt ggaaacccac
     1501 agtatacacc gggttgaggg atgtcatggg ttcctggaat atcatggaga tgtcgccgcc
     1561 ccgcaccttg cacatgtcgg gctccgaaag ttcgagaagg ttccttcctt ccaagagcac
     1621 gcggccggac actatgaggc ccggcggaga cgggatcagc ctcatgatgg agagagcggt
     1681 cgcgctcttc ccgcaacccg actcgcctac aagagccagc gtctcaccgc gttgcactgc
     1741 caacgatacg ccgtctaccg catggatcgg gccgcgttca gtaagatacc atgttacaag
     1801 atcttctacg acgagtgtag gctcatccaa tgctctcgcc tccccccggc ctcccgatgc
     1861 ctgcctgttt gccgatccct gcgtacctgg ccattctctt tctcaacttc gagtaagcag
     1921 actgcctggg gatatggggg tttagaacct cttccagagc gtacccgaac atcatgaaac
     1981 cgaagacggt tatgaatatg gcaagtcccg ggggaatgac ccaccaccat gcgccaaaca
     2041 gcaaggcatg gcccgactgc gcctcataga gcatcttccc ccagctcact gcggttggat
     2101 ctccgaagcc gaggaaactc agcccggcct ctgccaccat cacgccggca gcggagagca
     2161 ccccatttac gatgaggagc ggcgagactg ataccagtat gtgttcgcgc atgatgtgcc
     2221 cgggcctcgc cccgacgctt cgcgacgcaa ccacgaagtc gcgcgacttg agcgagagca
     2281 cctgcgaccg tatcatccga gcgacgcccg cccagcccag catggtgaag atgacgatta
     2341 tcatgtagaa cttagtgccc atgtagttcg ccaggagtat catgagcggc aaccccggca
     2401 ctacaaagag gatgtcgcaa gtcctcatta ttagggtatc ggcgaaccct cccaagtagc
     2461 ctgctacgat gccgagtagc gctcccaaca cggtaacgca caccccagtg gtaataccga
     2521 cgatcagcga gacccgggct ccatatatca acttgctcag gatgtccttg ccctgagcgt
     2581 cagtgcccag gggatgcccc ggtcccggag gcagtcgtct gggccctctc tggccaatgt
     2641 cgtacggatc gtacggtgcg atccacggcg ctgcaatcgc aatgatgatg accgcagccg
     2701 ttattatgat cccgacccgt ccgcgggggt gtctccatag tcgactggca atgtcgtgca
     2761 cgtcatgtcc cccctacttc agcttaaccc gtgggtccag ccgagcgtag gcaaggtccg
     2821 cgcacaggtt ggccaggatt gtaatgacag ccattatcaa catcgtcccc tgcatcaaag
     2881 gatagtccaa agaccgggag gcctccacga tcaacaggcc catcccttcc cacgagaata
     2941 tggtctcgat cactgtcgct ccgcccacaa gcccagacag gctcatgcct attcgggtga
     3001 caagcggaag cattgcattg cggagagcat gcctgtaaag agccgtgttc tcggataggc
     3061 ccttcgcgcg cgcggtccgg acgaagtctt cgccggatat ctctaccatg gagttgcgca
     3121 ccaggatccc atagcccaca atcccgccta tgcacatcga cagcactggc aggaatgcgt
     3181 gcctagccac gcttccgatg aatgcccagt tccaccccac tggaacgtgc gggctgacca
     3241 tgccgtaggc cggaaagacc ggccagtaga aggccagtat cagtgtgagt atcatggcaa
     3301 tccagaaaga cgggagggct gacacaacga tcgaagcaga aagcaaaatc gtatcgagat
     3361 gccctcccct cttccaggct gcggcggcgc cgagcgcaac ccccagcgcc accgacacca
     3421 ggaaagacga tagagtaagt accagtgtcc acggtatgcg cgagagtatg atctcgctca
     3481 cagggcgcct gtaggagaag gaggtcccga gctctcctcg gagcaggttc ccgaggtata
     3541 ctgcatactg tacatgaagc ggcttgtcga ggccaaactg cgccagtatc tcctgcttgg
     3601 cctctggcga aagccgcgga tcgttataga ggtgccccgc aggagtcccc ggagtgagcc
     3661 gcggcaggaa gaagttgagc gtcaccacta tgaagaggat cagcgcggtc tggagcagcc
     3721 tccttgccca ataagcgccc gacactatta ctttcctccc ttgtagacca ggttggagta
     3781 ggtcgtcgaa ttgttgatac agctatcggg ctctacggtc caccccgtga aacggtcgtc
     3841 cctgtagagc atgagtacgt tgctgctgaa cagcggaatg tggtaatagg cctcagccaa
     3901 cttggcctgc atatcgcgaa gaagcttgat cctcgcctgg tcattcatct cgaccaacat
     3961 ggcctcggca agcttgtcgt attcagggtt ggagtaacct ccgtagttga accccgcaac
     4021 acgcccttca ttgatgacgc cgcgcgaagt gtgtgcatag tagaacatca tgtcaggcac
     4081 ggagaagctc acgctcgaca ccgcagcatc gaaatccgca gcctgctcct tattcctaag
     4141 cgactccgca gtgagcggag tgaaatacgc atcgattcct atctgcttta tctgatatac
     4201 gagcagctct gccaggttta tgctggttgg cagattgccc gtgagtatct ccaacttgat
     4261 gggtttgccg ttcttgtcct ccctgatgcc gtcgcggttt ttgtcgacat atccggcctg
     4321 atcaaggatg gccttcgcct tcgcaacgtc gcgcggcacg gggccaaggg ccttgttgta
     4381 gtgcagatcg ctcggaacga cgagcccagg gccctgctca ctgccatagc ccagcagcac
     4441 ctggtcgatg agctccttct ggttgatggc gagcgatacc gccttgcgca gcgtcgcgtc
     4501 attccacgga gcgcgcctca agttgaacat cagcgtcctc acggaggtgc tgggcgccat
     4561 tgccaccttg acgttcggat aacccttggg attcctgtcg atctcctccg ccactgccgg
     4621 atcgacactc cagagaagcg catcaagatc gcccctacgg agggccatta tccccatggt
     4681 ttcgtcgcgc agcacccgga gggtgatggt ttcaacattg aggctggacc gatagtggtt
     4741 gcccaggttc ttcagcgtta tgaactgacc gtcgcgcctt tctgcaactg cgcacggtcc
     4801 gcatcctatc gggttatcga gattggggta gtttctcggg tcgtctatct tgctccaaat
     4861 gtgcttgggc aagatagacg gagcggtggc gatgaagcgg accgcgttcg atgagggact
     4921 gcggaatgtc acacgcactg tgtgtttgtc gattgccttc gcccctgcga gattccctat
     4981 gatcgccatg aagcttggga accgcttctc gcgaatgaag ttgaagctga actccacatc
     5041 ttcagcagtg acgggcttcc cgtcatgcca cttcgcgttc gggttcaact tgaactcgac
     5101 tgacgtccct ccgtctttga tctcccagct gtcggccagg cccggaacca agccttcgtc
     5161 ggagtcccag ccaagaaggg tgtcgtacac ccatccgatg acgaacgttt cagagcttcc
     5221 gtagcccatg tatgggttga ggttcttgac gtctgctatc actccaaggt tgaggttgcc
     5281 gacggccttg gccgcccccg ctaccccagc gcccgtcgga accatcagaa ggccgacaca
     5341 gaggatagcg attaccacac tcctaagcgg cttttccatg cgcactacct ccttgcttcg
     5401 caatcctgcg tcttgcgtct acagaatctt cgtcccaaaa gcattggggc caacgctgtt
     5461 gtcgaatatc tcttctatgt gaatcacata gaccggcctc ttctgccaca gatcagtgtt
     5521 gggtttgtgg tacacatcgt gctgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnagaccg gcctcttctg ccacagatca gtgttgggtt tgtggtacac atcgtgctga
     5701 gcgttgatga atttgtgata cttgccgtct ctgtgaatct ccatcttccc gcgaacctca
     5761 taggtgatgg cgggtggctg atagtacacc agcgttactt ccgggttttc gagatagttg
     5821 gtccaagaat gcttgagtgc cagctcgatc gagcccaaaa tcgagaaatc gatccgctcc
     5881 ttgcatccct cgccccacat gtacttcacg agtagctgca acccctcttc gctgtaccca
     5941 tcgcgccaac cgcggccgat gtgctcgagg taggcatcaa ggacttcgtc caggtactca
     6001 gccttgggta tgaaccctac acctttgata gaaccgttga ggccggcgct gccgttggat
     6061 atgaacgtag gagtatgtcg ggtaaacccg aggaaaacct cgtctcgact gacttgatcg
     6121 ccctgagctt gccttcgcac cagctcggcg cgaccgtcgt aggcccatct cacaaacttg
     6181 tccggcaatc tgaattcgtc catacactaa cctcctagta ctgcgcaatc tgctcagcgg
     6241 gcgtggtttg agattggtct gtggttacgc gttaactacg cttcttcgag gcagcagctg
     6301 ataatcctcc cattctccga gctaacttct atcacgcgcc gttcgaggcc tcgcctatca
     6361 accccagttg tgtcctgtat ggctacccta gccatcgcaa gaaggattag gaacggaaaa
     6421 ggcgaaatca gtggcgaatt gggccccatc gggcggattg agatggtcgg cattgaccaa
     6481 ctccgctgac gcgcatctgg atagagccat cttgtgcgta gactcctaca tctacttcgg
     6541 tactgacgat cactttgtca actacatcct aaagttcctt gcgcccggcg gaacgctggc
     6601 cgtggccatg cccggactca tgaaggattc tgaggacggc gtctcacacc atctaaagcc
     6661 gttctggggg caggactgct ggtgctggca tagacttgaa tggtggagac acctctggga
     6721 gcgcactgga ctggtggaca tggtcgaagc cgacacgctt ccggatggat gcgcctcgta
     6781 cgtacgctgg agagaagccc aggacaaggc gggcctgaac ccgtggcccc aggacattgg
     6841 gatcttgaag caagacgcgg gagaatatgt aggattcatc aggctagcag cgcgcaagac
     6901 gtaggttatc gtcgcgtatc gaggctccgg atcaccgcat tgatccgcct tctcaccttc
     6961 tggtattcga tttctgccca cgccctgagc ctcttcggcg gtcaaggcgc caaggtagtg
     7021 caacgcttca tgcctaggaa ttccgtcccg catgacggac tcaacgatgg tgccgttcgt
     7081 ctcgaccgcc gtcaatgata tcacagcggg gagcacagtg taaaccaata attcccacat
     7141 gcgcgtcatg agaaggaaag tccgcagcag gtgtactacg caatcgcata tgatcctctg
     7201 accgcagaga ccaaagacat gacaagctcc tgtttgacaa gaaccgagac tgccttctgg
     7261 gtcagtccat cccgggcccg gcaggccccg aggggttcta ccggcgactt gggctcgagc
     7321 ccacggagat cgctacgggg accagccgga atacgttcta ggcctggacg agtgagctcc
     7381 gaagacgaca aggcaacgtc gctgaagcct gatcactatc tcatgtagcg tttcagttgc
     7441 tcgtagaagt tctctc
//
LOCUS       DAOTAC010000105         7431 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_8464_length_7431_cov_4.558568, whole genome shotgun sequence.
ACCESSION   DAOTAC010000105 DAOTAC010000000
VERSION     DAOTAC010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7431
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8464_length_7431_cov_4.558568"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="PLM74_05775"
     CDS             <1..741
                     /locus_tag="PLM74_05775"
                     /inference="COORDINATES: protein motif:HMM:NF013314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HON42628.1"
                     /translation="SIAGKAGAREISGSLFEAMSGSCELQKKSLAPWLTRNLEESGVL
                     VIPIPRYMPRDDRSAPKACQQYSLPEFYDNIVLLDTGHAKLMAPFFPMDRLRAVPGFE
                     SALYQDPYAGGRGNSIRFAVITPHDLSMKVTGVENLFCAGEKAGLMVGHTEAICTGTL
                     AGHNAVRKAVGKEPLILPSSLACGDLLACIERSLGSPSGLLSKYTFAGGVYYNRMYDS
                     GLYTTDIRRISQRVRDAGLAGIFARRLL"
     gene            complement(758..1591)
                     /locus_tag="PLM74_05780"
     CDS             complement(758..1591)
                     /locus_tag="PLM74_05780"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HON42629.1"
                     /translation="MNHVDRRRLPVWLFAIAVTFLLAMGALPYPAYAKTALPARPEPA
                     RLMTDLAGMLGRSAVDEIESQLVVLWQDTGVQFTVVTVPDLGRDTIDNYSVRLFEEWG
                     IGEAGKDNGLLLLIASEEREMKFETGYGLEGELPDSFLGKVIDQALVPKFKAGDPAAG
                     IKASITMVSDRLKGRAGEGPGSDESSDSIGEIAAVIVFLVVAMVIIAISNRRRGPRGK
                     GPGSGSGGGGSTWRTPMTLPKSTGGWGGHSGGRSGGSSSGGFGGFGGGRSGGGGAKGG
                     W"
     gene            complement(1611..2177)
                     /locus_tag="PLM74_05785"
     CDS             complement(1611..2177)
                     /locus_tag="PLM74_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004080601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HON42630.1"
                     /translation="MKRTGLVVLIVLLALVVLFASSFVRISNSIVRAEEDVNVQWSEV
                     QNQLQRRYDLIPNLVETVKGYAAHEEEVFTAIAEARAKLGGARTVDETAQAVNQLEGA
                     LSRLLVVIENYPQLKADAHFSELMYELSGTENRIAVARMRYNESVQSYNVYIRVFPNS
                     IVAGMKGVSPKQMFEAAEGAERAPEVKF"
     gene            complement(2341..3255)
                     /locus_tag="PLM74_05790"
     CDS             complement(2341..3255)
                     /locus_tag="PLM74_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006772607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HON42631.1"
                     /translation="MHLVIEMLRSSVLAATPVLLAGLGGTYTYYANVFNIAMEGMMLI
                     GAFTAVAGSYLAGSWVVGTLAGMVGGVVAALVFAVFAVYLKTDEFVTGTAINMLALGG
                     TTYALRQMFRVKGAFMSPMIRAIPRIRIPLIGALPGIGPVASGHPFLVYVTLALAIVI
                     EWHMFRTRYGLRLRAAGEDPRAVDSVGVSSDRIRAQAILISGALCGLAGVYLSLGYVT
                     LFAENMSNGRGWIALAVIILTRGRPLGLLTMSLVFGFFDGLGLSLQSTRIPPQFTQMV
                     PYIATIVALYLYSREKKRVSAGQAGQAG"
     gene            complement(3239..4333)
                     /locus_tag="PLM74_05795"
     CDS             complement(3239..4333)
                     /locus_tag="PLM74_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HON42632.1"
                     /translation="MSKNAITALAGIVVALLAGLAILWAQGYPALPSYAALFSYSLFS
                     KFAIVSTLNKATPLVLTGLSAAVGFGSNCVNLGQPGQFLIGAMAVAAFGIACDLPTPV
                     MIPLAIMVALAAGALWAGIAAGLRRRFNMDEFITTLMLNFIADYFTLYLISGPMWDRT
                     TYSPMTKMTNPGSWLPMWDGLSAAFVVSCVAFVITWVIWDRSKLGYEWRVMGQNSAFA
                     RVGGCRNEANFTRAMLLSGALAGLAGGLMIMGGMQHRFVKGMGANYAWDGVMIAIVAA
                     NGMVPTGLYALFFAAIQTGAMGMELETAVPSEFALVLQAIIVLFVVASRESTRLLINR
                     WAAKLRARAAAGGAQDIGKGEGGERRASGH"
     gene            complement(4330..6024)
                     /locus_tag="PLM74_05800"
     CDS             complement(4330..6024)
                     /locus_tag="PLM74_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON42633.1"
                     /translation="MAQKVLLCVLFDILEVPSHLDDHFIEMRSLIKVYPPDTLALDRV
                     DLTIKEGEIHSLIGENGAGKSTLMKVLYGMIPASSGEISFRGRRVRYASPREAVATGI
                     GMVHQEFMLVPSYTIYENVILGAEPVGRLGALRNAEARRRVQSLIDRYGLNLNIDDRV
                     CDTSVGAQQKVEILKLLYRDVDVLIMDEPTAVLTPQETGELFRRLRDLNAAGKTIIFI
                     SHKLDEVLELSDTITVMRRGRHVATWENRGLGKADLARAMVGRDVVFSVEKPPACCGD
                     VVFEARDLSYVDAGGRRRLDGVSFTVRRGEIVGIAGVEGNGQFELVQLVTGLAPVQSG
                     QVKICGRDVTRAPVIERRKLMCYVPQDRKASGSAQKMSLTDNSIMTHHRLNRRIESGW
                     RALLNPAKCRRFAQNLVESFGVLAPNVDTPISSLSGGNQQKVVVGREFLFDADFVLLD
                     QPTRGVDVGSVEFIQREIIRKRGSIEGATGDVGADATVTATATAAAGCLLISADLDEL
                     FNLADRILVMHRGRIVADLAPPDTTREEVGEYMLGAREPQPGPWGGAQAGEGACAR"
     gene            complement(6124..7134)
                     /locus_tag="PLM74_05805"
     CDS             complement(6124..7134)
                     /locus_tag="PLM74_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012002723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HON42634.1"
                     /translation="MKNRLGVVFVALAIVAALAVGASSAAPKKVAYIINGALGDLSFY
                     DSGQAGIDRIAKDFKVQTRTIECNFDPAKYPQALQAAIQWNADAIFVISYGYEDLLMQ
                     YADQYPTKYWVNVDTVVENKKKTITSVDFVEEEGAFMAGVVAGLVTTDTSIRGVNPDK
                     VIGAVGGEDDPVIRSFIYGYEQGAKYIDKDIEVKTVFAGTWDDPVRGKQAAKQLYSQK
                     ADIVFQIAALTGSGVLEAAREEGKYAIGVDSNQNPLQPGHVVTSDLKNVGDAIYGVFK
                     TIVDGTYKPGVVLEYGLKEGGVGLAIDEHTEKILPAKSLQRVLEIQEKVKSGQIKVKR
                     YQ"
BASE COUNT         1505 a         2423 c         2045 g         1458 t
ORIGIN      
        1 agcatcgcgg gaaaagccgg ggccagggag atatcgggca gcctgttcga agccatgagc
       61 ggttcctgtg aactacagaa gaaatcgctg gcgccgtggc tcacacgcaa cctggaagag
      121 agcggcgtac tggtgattcc gataccgcgg tacatgcccc gagacgatcg gtctgcgccc
      181 aaagcgtgtc agcagtactc gttgcccgag ttctatgaca acatagtcct gctcgatacg
      241 ggccacgcca agctgatggc gcccttcttc cccatggatc gactccgcgc cgtccccggc
      301 ttcgagtcgg cgctctacca ggacccttat gcaggaggca ggggcaattc catcaggttt
      361 gcggtgatta ccccgcatga tctatcgatg aaggtgaccg gagtggagaa cctattctgt
      421 gcaggcgaaa aggcaggcct catggtaggg cacaccgaag ctatctgcac gggcacgcta
      481 gcaggacaca atgcagtgcg aaaagccgtc ggcaaagagc ctctgattct accctcatcg
      541 cttgcctgcg gcgacctgct cgcctgtatc gagcggtcgc tgggctcgcc ctcggggttg
      601 ctcagcaagt acacctttgc cggcggcgtg tactacaacc ggatgtatga ctcgggattg
      661 tacaccaccg atatccgccg catctcccag cgggtcaggg atgccggcct cgcaggcatc
      721 ttcgcacggc ggttgctctg agcgcagacg ggcgaggcta ccacccgccc ttcgcgcctc
      781 cgccgccaga gcgcccgccg ccgaagccgc cgaagcctcc ggacgaactg ccgccagatc
      841 ggcctccgga gtgacctccc cagcctcctg tagacttggg caaggtcatt ggggtacgcc
      901 acgtgctgcc tccgcctccg ctgccgctgc ccggcccttt tccgcgcggg ccgcgacggc
      961 gattggaaat cgctatgatg accatggcga cgaccaggaa gacaatcaca gcagcgattt
     1021 cgccaatgga atccgaggac tcatcgcttc ccggcccttc gccggctcga cccttaagac
     1081 ggtcggagac catggtaatg gatgccttga tacccgctgc cggatcgccc gctttgaact
     1141 ttggaacaag ggcctgatcg atcactttgc cgagaaagga gtcgggcagt tcgccttcga
     1201 ggccgtaacc ggtctcgaac ttcatctccc tctcctcaga agcgatcagg aggagcaggc
     1261 cgttgtcctt gcctgcctcg ccgatacccc attcctcgaa cagccgtaca gagtagttat
     1321 cgatggtatc gcggccaaga tcaggcacgg tgaccactgt aaactgcaca cccgtatctt
     1381 gccagagcac gacaagctgg ctctcgattt cgtcaacggc ggaacgcccc agcatacccg
     1441 caagatccgt catgagacgc gcgggctcgg gccttgcggg gagtgccgtc ttcgcatagg
     1501 ccggataggg caaagcgccc atggccagca ggaacgtcac ggcaatcgcg aagagccaaa
     1561 ctgggagacg tctgcgatct acgtgattca cgtgtgtgcc ctccgcttta ctagaacttc
     1621 acctcgggcg ctctttccgc gccttcggct gcctcgaaca tctgcttggg cgaaacaccc
     1681 ttcatgcctg ctactatcga gttcggaaag accctgatgt acacgttgta cgactggacg
     1741 gactcattgt atcgcattct agcgacagct atccggttct cggtacccga cagttcatac
     1801 ataagctccg aaaagtgcgc atccgccttg agttgagggt agttctcaat taccaccagc
     1861 aagcgcgaca gggcgccttc cagctggttg acagcctgag ccgtctcatc aaccgttctc
     1921 gccccgccaa gcttggcccg ggcctctgct atcgcagtga agacctcttc ctcatgggca
     1981 gcatagccct taaccgtttc cacaagattc ggaatcaaat cataacgtcg ttgcagctga
     2041 ttctggactt cagaccactg gacgttgacg tcttcctccg cccgtactat ggagttggat
     2101 atcctcacaa agctggaagc gaacagaaca accagcgcca gcaggacgat gagcaccacg
     2161 agcccagtcc ttttcatggc atgcgccccc tctcaatacc ttcgagtagt ttcggaatgc
     2221 ctgtcggacg gttatagatt aacacgctta atgcctaacg acaaccgatg gtcaatcgtt
     2281 cccagccttc taggcccacc cggcccactc ggcctgctta gcccacgtgg cccgctcggc
     2341 ctacccggcc tgcccagcct gcccagccga tacgcgtttc ttctccctgg aatacaggta
     2401 aagagctact atagtcgcga tgtacggcac catctgggtg aattgcggcg gtattctggt
     2461 gctctgcagg ctgaggccca ggccgtcgaa gaacccgaac accaacgaca tggtgagcag
     2521 ccctaaggga cgaccgcggg tgagtatgat caccgccagc gcgatccaac cgcgtccgtt
     2581 cgacatgttc tccgcaaaca gtgtcacgta gcccaacgac agatagacgc cggccagccc
     2641 gcatagcgcg cccgagatca gtattgcctg ggctcgtata cggtctgagc taacgcccac
     2701 ggaatccact gccctcggat cctcgccagc cgccctaaga cgcaggccgt agcgtgtgcg
     2761 gaacatgtgc cactctatca cgatcgccag agccagtgtc acatagacga gaaacggatg
     2821 cccgctcgct accggcccga taccgggcag cgcgccgatg agaggaatgc gaatacgcgg
     2881 tatggcacga atcatgggcg acatgaacgc acccttcaca cggaacatct gcctcagagc
     2941 gtaagtcgtg cctcccaggg ccagcatgtt tatggctgtg cccgtgacaa actcgtctgt
     3001 tttgagatat acggcgaata ccgcgaacac cagggcggcc accacccccc ccaccatgcc
     3061 tgccagcgtg ccaacgaccc atgaacccgc gaggtaactg ccggccactg cagtgaacgc
     3121 gccgatcagc atcatccctt ccatggctat gttgaacaca ttggcgtaat acgtgtacgt
     3181 gcccccaagc ccggcaagaa gcaccggcgt ggcggcgagc acgctcgagc ggagcatctc
     3241 aatgaccaga tgcacggcgc tcaccccctt ctcccttgcc gatgtcctgc gccccgccgg
     3301 ccgcagcccg cgcccggagt ttggcagccc acctgtttat gagcaatcgg gtcgattccc
     3361 tgctggcaac gacaaagagc actatgatgg cctgcaagac cagcgcgaac tcgcttggaa
     3421 ccgccgtctc cagctccatg cccatggcgc cggtctgtat ggcggcaaag aacagagcat
     3481 aaagccctgt aggcaccatc ccattggccg ccacaatggc gatcatcacg ccgtcccaag
     3541 cgtagttggc gcccatgcct ttcacgaacc gatgctgcat gcctcccatg atcatcagcc
     3601 cgcccgcaag ccccgccagg gccccgctca aaagcatggc gcgagtgaaa ttagcctcgt
     3661 tgcggcaccc tcctactctg gcaaacgcag agttctggcc catcactctc cattcatagc
     3721 ctagcttgga ccggtcccat atgacccacg tgatgacaaa cgcgacgcac gatacgacaa
     3781 acgccgcgct gagcccatcc cacatcggca gccaactgcc tggattggtc attttggtca
     3841 tcggcgaata tgtcgtgcgg tcccacattg ggccggatat gaggtagagg gtgaagtagt
     3901 cggcgatgaa gttgagcatc agggtcgtga tgaactcgtc catattgaac cgtcggcgca
     3961 gccctgcggc gatccccgcc cagagcgccc cggcggccag agcaaccatg atcgcaagag
     4021 ggatcatcac gggtgtcggc agatcgcagg cgattccaaa cgccgcaacc gccatggcgc
     4081 cgatcaagaa ctggccaggt tggcccaggt tcacgcaatt gctgccgaat cccaccgcag
     4141 ccgaaaggcc ggtcaggacg agcggcgtgg ctttgttcag tgtagatacg atcgcgaact
     4201 tagagaaaag actgtaagag aaaagcgcag catagctcgg caacgccggg tatccctgcg
     4261 cccacaggat cgcaaggccg gccaggagag ctaccactat cccggctaga gctgtaatcg
     4321 cattcttgct catcgggcgc atgccccctc tcccgcctgg gccccgcccc agggcccggg
     4381 ctgcggctcg cgggccccca acatgtactc gccgacttcc tcgcgggtag tgtcgggcgg
     4441 cgctaagtct gccacgatcc ggccccggtg catcactaag attcggtcgg ccaggttgaa
     4501 cagctcgtca aggtcggcag atatcagcag gcagcctgcg gccgcggtcg cagtcgcagt
     4561 cacagtcgcg tctgcgccca cgtctccagt cgcgccctcg atcgaacccc tcttgcggat
     4621 gatctctcgc tggatgaact ccaccgagcc tacgtccacg cctctggtag gctggtcaag
     4681 caggacgaag tcggcgtcga agagaaattc gcgaccgacc accaccttct gctggtttcc
     4741 acctgaaaga gatgatatgg gcgtgtcgac gtttggggcc agaacaccga aagactcgac
     4801 caggttctgc gcgaagcgtc ggcacttcgc ggggttcaga agcgccctcc accccgactc
     4861 gatccgccga ttgaggcggt ggtgcgtcat tatggaatta tccgtcaggc tcatcttctg
     4921 cgcactgcca gacgccttgc ggtcctgcgg cacatagcac atgagcttgc gtcgttcgat
     4981 gaccggcgcg cgagtgacgt cccgaccgca gattttgacc tgccctgact ggaccggcgc
     5041 caagcccgta actagctgca ccagctcaaa ctggccgttg ccttctaccc cggctatccc
     5101 aacaatctcg ccccgtctca ctgtaaacga caccccgtcc agtctgcgcc tgccgcctgc
     5161 gtccacatag ctcaaatcac gggcctcgaa caccacatca ccgcagcaag cagggggctt
     5221 ctctaccgag aacactacgt ccctgcctac catggcccgg gcaagatctg ccttgccgag
     5281 gccgcgattc tcccaggtgg cgacgtgacg tccgcggcgc atcacagtga tagtgtcaga
     5341 tagctcaagc acctcgtcga gcttgtggct gatgaagatg atcgtcttgc cggcagcgtt
     5401 cagatcgcgc agacgcctga agagctcccc cgtctcctga ggagtgagca cagcggtagg
     5461 ctcgtccatg atcagcacat ccacatcacg gtatagcagc ttgaggatct caaccttctg
     5521 ctgtgcgccc acagacgtat cgcatactct atcatcgatg ttgaggttca ggccgtaacg
     5581 gtcgataagc gactgcacgc gtcggcgcgc ctcggcattt cgcaacgcgc ccaaccgccc
     5641 cacgggctcg gcgccgagta tcacattttc gtagatagta taagaaggca ccagcatgaa
     5701 ctcctgatgc accattccga tcccggtcgc caccgcctcg cgtggactgg cataccgcac
     5761 gcggcgaccg cggaagctga tctcgccgct gcttgcaggg atcataccat acaaaacctt
     5821 catcaaggtc gacttgccag ccccgttttc gccgatgagc gagtggatct cgccttcctt
     5881 gattgtcagg tcgacgcggt ccagagcgag ggtatcgggg ggatacacct tgataaggga
     5941 acgcatctct atgaagtgat cgtccaagtg acttggcacc tccaggatat caaacaagac
     6001 gcagagcagg accttctgcg ccactgatcg gccctgcccc gcgccggata cacttcgcgt
     6061 gggttcacga gcacttcggc tgccccaagt gccggaattt gcccgcgtgt cccagattat
     6121 ccgctactga tacctcttca ccttgatctg ccctgacttg accttctcct ggatttccag
     6181 gacgcgctgg agcgatttcg cgggcaggat cttctcggtg tgctcgtcga ttgcgagccc
     6241 cactccgcct tccttgagac cgtattccag caccacgcct ggcttatacg tgccgtcgac
     6301 gatggtcttg aatacgccgt agatggcgtc tcccacgttc ttcaggtcag aggtgaccac
     6361 atgccccggt tgcagcgggt tctggttgga gtcgacgccg atcgcgtact tgccttcctc
     6421 ccgagccgcc tccagcacgc ccgaaccggt gagcgcagct atctggaaga cgatatctgc
     6481 cttctgcgag tacagctgct tggcggcctg cttgccgcgg actgggtcgt cccacgtccc
     6541 ggcaaagacc gttttcacct cgatgtcctt gtcgatgtac ttcgcgccct gctcgtagcc
     6601 gtagatgaac gagcgtatca caggatcgtc ctcgccgccc accgccccga ttaccttgtc
     6661 ggggttgacg ccgcggatgg aagtatccgt ggtcaccagg cctgcaacaa cgccggccat
     6721 gaaagcccct tcctcttcca caaagtcaac gctggtgatg gtcttcttct tgttttcgac
     6781 cacggtgtcg acgttcaccc agtacttggt gggatactgg tcggcatatt gcatcagaag
     6841 atcctcatag ccgtaggaga taacgaatat ggcgtccgcg ttccactgta tcgcagcctg
     6901 aagcgcctgc ggatacttgg cggggtcaaa gttgcactcg atagtgcgag tctggacctt
     6961 gaagtccttc gctatcctgt cgatgcctgc ctgcccggaa tcgtagaagg acagatcgcc
     7021 cagagcgccg ttgatgatat acgcgacctt cttgggtgcc gccgacgacg cgcccaccgc
     7081 cagcgccgcg acgatcgcaa gcgccacgaa cacaactccc agccggttct tcattgttca
     7141 ccctccgata accgcgccgc gcagcgcggg tttgtagttt tcggcgcact gcttccgccg
     7201 tgcgtcgcag tatactgtgt ttacttcgtc gccattgcga ttaatcctgt attccccgaa
     7261 gctgggatgc gttcgttttc cgaacgggtt ttcggggaag cgttccgaaa acatgcacct
     7321 agattctacg gggagataac acgctgctgt cggcgtagtc aaatagctcc actttgagct
     7381 gacccgtgcg gcgaccgtag agcgggctcg catagccaag cacttggttc t
//
LOCUS       DAOTAC010000106         7349 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_8595_length_7349_cov_4.771593, whole genome shotgun sequence.
ACCESSION   DAOTAC010000106 DAOTAC010000000
VERSION     DAOTAC010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7349
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8595_length_7349_cov_4.771593"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(227..1408)
                     /locus_tag="PLM74_05810"
     CDS             complement(227..1408)
                     /locus_tag="PLM74_05810"
                     /inference="COORDINATES: protein motif:HMM:NF018591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HON42635.1"
                     /translation="MAGAGLWDWLRGYVIIRIEGRAPARFVNMAAAYRIELADVTMLG
                     PGMMLVRTSVDGFRRLGPVLRASGCRAAIEERVGAAFIVARLLRRRFLVLGAVLFLAV
                     LYGSSSVLWTIEVVGAEPPERAQILQVLQGMGVRRWTMRHRVDCDAIRREVTRSLLQL
                     AWVGVELRGTMLTVRVVPKVAPEVPEGPVDIAASADAVITRLILLAGDAVAREGDTVV
                     RGQIIVRGRAGPGAGDRPVHARALVQGRVWRAGQARIPLTIEQRLRTGRTADQYVLRL
                     AGAELHLGRTGRFEAYDTEQFSKTLVPGRDGGSLVEVIRIRRHELSVELIQLSREQAY
                     AAARDCALSTAMSDVPAEARVVMISEEVSDQSLMPDAVDVRIVIETLEEIGVQQVQQL
                     R"
     gene            complement(1410..1901)
                     /locus_tag="PLM74_05815"
     CDS             complement(1410..1901)
                     /locus_tag="PLM74_05815"
                     /inference="COORDINATES: protein motif:HMM:NF019488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HON42636.1"
                     /translation="MRSHHKRRRAGRLGGAELSGGVASRSRDSVAGRSEPAGRPEPAS
                     RSGVPGRHGADTAGPHRIPGARRISDALGVPVDIALNMPRMNFEGNARLVLDNHRGLV
                     EYTPSHLQIDTPAGQIDITGKRLRIRGISRDQIVADGWFDRVEFSGWVGNATAGSAGQ
                     EAP"
     gene            complement(2298..2486)
                     /locus_tag="PLM74_05820"
                     /pseudo
     CDS             complement(2298..2486)
                     /locus_tag="PLM74_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011235908.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(2919..4337)
                     /gene="purD"
                     /locus_tag="PLM74_05825"
     CDS             complement(2919..4337)
                     /gene="purD"
                     /locus_tag="PLM74_05825"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HON42637.1"
                     /translation="MRTPDSSSRLNVLVVGSGGREHAIVWKLARSPRIGRVIAAPGNP
                     GIGRLAACAPVGATDVAGLVRLAREAECSLVVVGPEAPLAAGLADAMRAIGVTVFGPS
                     AAAARIESSKHYAKGVMARAGIPTAHCEAFTDVGRARDAARTIAQEAGGVVVKLDCLA
                     AGKGVVVADSPEEAGRAVDELGAMGAGRGCKILVEERLVGPEASVMCLSDGADIWVLP
                     PARDHKRALDGDQGPNTGGMGAVAPASLVYEATQADLGVTLTNGTAQLARDAILADRA
                     MLAEIERTILKPAIDQMAVEGTPFVGALFAGLMLASEGPKVLEFNCRLGDPETQAVLP
                     LVDVDLLDLVEAACHGQLRGVRAGTGPAQHAPVAGSGGAFNGTSDGLHSTCVVMASPG
                     YPGPHRTGIPVSIPDPLPEGVVAFHAGTALDDGQLVSAGGRVIGVVGIASSAAQSRKL
                     AYDVVGRIGFEGAHYRRDIGAI"
     gene            complement(4350..5957)
                     /gene="purH"
                     /locus_tag="PLM74_05830"
     CDS             complement(4350..5957)
                     /gene="purH"
                     /locus_tag="PLM74_05830"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HON42638.1"
                     /translation="MSVYDKEGVVEFGRALADLGFEIISTGGTASALRRAGLGVTDVS
                     QVTGFPEVLDGRVKTLHPRVHAGILARPTPEHMQALEELDVVPIDIVAVNLYPFQHTV
                     DSGADEAEIVENIDIGGPSMARAAAKNFERVAVIVDPADYPVVVAELRENGSVSGSTR
                     VRLAAAAFQHTAAYDAAIARYFADSMAGVPRQCEQREADEGREAGQTGLAGGEREAGQ
                     GLGREFTLGGVMIQTLRYGENPHQMAAFYRSVGERSWGLAAAKQLGGKELSFNNIGDA
                     DAAVRAVAEFDETAVVIVKHANPCGIGVGANVAEAYRAALDSDPVSAFGGIAAFNRPV
                     DLETARLMSEIFLEVIIAPGFVPEAVELLHGKKNLRLLELAVDDITAGTGWDVRSVAG
                     GFLVQEPDRVGHLDMRGLPVVTRRAPDEGEMVQLANAWKAVAHVKSNAIVLWRGNGTV
                     GVGAGQMNRVGSARIAMEQAGQRAEGSVMASDAFFPFPDTVEVAAKAGVTAIIQPGGS
                     LRDKESVQVADAHGIAMVFTGRRHFRH"
     gene            complement(5965..6672)
                     /gene="purN"
                     /locus_tag="PLM74_05835"
     CDS             complement(5965..6672)
                     /gene="purN"
                     /locus_tag="PLM74_05835"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HON42639.1"
                     /translation="MSIRLAVLVSGRGSNLQAIIDAIRSGRLQASAEIVVSDAADAPA
                     VARALEVGIPAVVVRRRDYSSRAEFELALAGAAEKARPDLVVLAGFMRLLGPAFLDRF
                     PGRVINIHPSLLPAFPGLEAQRQALEYGVRFSGCTVHYVDYGMDSGAIIDQRVVEVMP
                     GDSVDSLSARILEQEHELLVDVIGRLGQQMNEHANEHANERVNARVGEQVGGKTGGKT
                     GRKIGEGRCARRGQEQA"
     gene            complement(6677..>7349)
                     /locus_tag="PLM74_05840"
     CDS             complement(6677..>7349)
                     /locus_tag="PLM74_05840"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HON42640.1"
                     /translation="ETAEMPGFYPDGEYDIAGFAVGAVDRRKVVDGSDIRPGDAVIGL
                     ASSGLHSNGYSLARKALLEQGGLTVGQSCDGLGTRRGSESAGTIGFASGCTLGDEMLR
                     PTRIYVRPVLALMEQLRVLGMAHITGGGLVENPPRMLPTGVSLRLEWGQWPVHPIFRM
                     IQEIGNVDAGEMRRVFNMGIGFMVVVRPGDAQQAVEILQANGENAYVIGDIVPGNGKV
                     LFEGA"
BASE COUNT         1366 a         2644 c         1960 g         1379 t
ORIGIN      
        1 ccttctccac tacatcctga gaccctacta cagtcacctc attggcacgg gccataatct
       61 tgcatccgag catcccttcg acgagtcgca agttttcgtc gcggttgccg aaaagctccg
      121 cagcttccgt attgccctca gtacagaatc gcgcctccac aaccggattg ctcaaatgag
      181 actgttccct cccgttttat cgaattcgtc gaatgggctc gacgacctac cttaactgct
      241 gtacctgctg tacgcctatt tcctctagag tttcgatcac gatacgcaca tccaccgcgt
      301 caggcatcag cgactgatct gatacttcct cactgatcat gacgactctg gcctcagccg
      361 gcacatcaga catagcggtg gacaacgcac agtcgcgagc ggcggcgtac gcttgctcgc
      421 gcgagagttg aatcagctca acgctgagct cgtgccgcct aattctgata acttcgacaa
      481 gactgccacc gtccctgccc gggactaggg tcttggaaaa ctgctcagta tcatatgcct
      541 cgaatctgcc cgtccgcccc aggtgcagct cggctcctgc gaggcggagc acgtactggt
      601 ctgccgtgcg ccccgtcctg agccgctgct cgatagtgag cggaatgcgc gcctgcccgg
      661 cgcgccatac acgtccctgc accagagcac gggcgtggac aggcctatcg cccgcgccag
      721 gcccagccct gccgcgcacg ataatctggc cccggaccac agtatcgccc tcgcgggcga
      781 ctgcatcgcc agctaggagt atcaacctcg ttatgaccgc atccgcagag gcagctatgt
      841 cgacagggcc ttccgggacc tcgggtgcca ccttagggac tacacgaacc gtgagcatcg
      901 ttccacgaag ctcgacgccc acccatgcca actgcaggag tgaacgtgtg acctcacgcc
      961 tgatcgcgtc gcagtctact cggtgtcgca ttgtccatcg ccgcacgccc attccctgta
     1021 gtacctgtag gatctgcgcg cgctcgggcg gctctgcgcc cacgacttcg atggtccaca
     1081 gaaccgagga agacccgtac aacacggcaa ggaaaaggac ggcgccgagg accaggaacc
     1141 tcctgcgaag gaggcgcgcc acgataaacg ccgctcccac ccgctcctcg atcgccgccc
     1201 ggcaaccaga ggccctgagc accgggccca gtcgcctgaa accatcgacg ctcgttctga
     1261 ctaacatcat acccgggcca agcatcgtga cgtccgccag ctcaatgcgg taagccgctg
     1321 ccatgttgac gaaccttgcc ggcgctcgac cttcgatccg aattataaca tatccgcgga
     1381 gccagtccca caatccagcc cctgccattt cagggcgcct cctgaccagc actgccagct
     1441 gtggcattac cgacccaacc tgagaactct acccgatcga accatccgtc cgcgacaatc
     1501 tggtcgcggc tgatcccgcg gatgcgcaga cgctttccgg ttatatcgat ctgccctgca
     1561 ggagtatcta tctgcagatg actcggcgta tactccacca agcctcggtg gttgtcgagg
     1621 accaaccggg cattgccctc gaagttcatg cgcggcatgt tgagcgcaat atccacaggc
     1681 accccgagag catcggatat ccggcgggcc cccggaatgc ggtgcggacc ggcagtgtca
     1741 gcgccgtgtc ggcccggaac ccctgaccgg ctcgcaggct ccggtcggcc cgcaggctct
     1801 gatcggcccg ctacgctatc cctagaccga ctcgcgacgc cgcctgacag ctccgcgccg
     1861 cccagccggc ccgcccgccg cctcttgtgg tggctccgca tccgcccgtc ccccctcccg
     1921 ccgcaccccc gcgccctcgc caaacggcgg ttgtccgttg gcgttccgct tcgggctacc
     1981 atccctgcgt cgtcattgca cgcattactc actctcaaga tgtatgcctc cacgcaggta
     2041 cgaatgacgg acggcagccc ctggtcgccc cgggctcccc gccacagaaa gcgcggtccg
     2101 cctcggccca cccggtgcac cagctgcctc cccagaaacg gagggaccat tttgactacc
     2161 gtcctacgtg aggcaacgaa tcagactcca ggttcacgtt gggctgaggc aacggtaaat
     2221 cgtccgtccc ggacggcaga tatgtcgcgg acgtgtagcc aggaccgtag ggaatccgcg
     2281 gacctgtgcc ctgtgtgcta tgcattaccc acgtcgatgg gggaattgag catctggctc
     2341 atgatcgacc aggtctgcga tatgtcttcc aggcgttgca gtccgcgcga gatcgtctta
     2401 acgcccggca tcccgtcatg cttgcatacc agatgtccgc cgagtttcgc tgtgagtctg
     2461 gccgcttcct ccagggtggg aggcgttcgg cgtctccatg gcttggtaag tgaaccagag
     2521 gaccttcagt gcgaaacagg gtttctgcag ctgagacacc aaactagcca gctcgatcca
     2581 tttcgcagta ccgtgtggcg tccggcgcga caggcccgaa gtcgcccact ttcgcgcttg
     2641 actcatgctc acggtctgtt gctcctccat cgtcatatgc gtcgggaaca tggtacgaca
     2701 tcatggcttt cacgttcaac ggcaccgagt gggcgaacat gctattcggg tctgtacgcc
     2761 caggaacgtc catggatgtg ggtaatgcac aggccctgtg ggccaggatt tccacggagc
     2821 ctgggtaaac ggacacagga tggcccaaga aggtgtcccg tagggcacag gaaccaatac
     2881 ctggcggtcc tgcttgccct ggggcccggc gtgcggcgtc aaattgcacc gatatcccgc
     2941 cggtagtgtg cgccctcgaa cccgatgcgc cctactacat cgtacgcgag cttccgcgac
     3001 tgggcggcac tcgaagctat gcccacgacg ccaatcaccc ttccgcctgc cgacactagc
     3061 tgaccatcgt caagcgctgt gccggcgtgg aatgccacga ctccttcagg caacgggtcg
     3121 gggatgctta ctgggatccc cgtcctatgt ggaccgggat accctgggct ggccatcacc
     3181 acgcacgtac tgtgcagtcc gtcggatgtc ccattgaagg ccccgcctga cccggccaca
     3241 ggggcatgct gagccgggcc tgtgcccgca cgcacacccc gcagctgccc gtggcacgcg
     3301 gcctccacca aatctagcag gtccacatcg accaagggca gtaccgcttg ggtttcggga
     3361 tcgcctagcc tgcagttgaa ctcgagcacc ttgggaccct ccgaagcgag catgaggcct
     3421 gcgaatagcg ctcccacgaa tggagttcct tccaccgcca tttggtcaat ggcaggcttg
     3481 aggatcgtcc gctcaatttc ggccagcatc gcccggtcgg ccagtatcgc gtcacgcgcg
     3541 agttgtgcgg tgccattcgt caacgtcaca ccgaggtcag cctgcgtcgc ctcataaacc
     3601 agcgacgcgg gggcgacggc gcccattccc ccggtattgg ggccctgatc cccatcaagc
     3661 gcacgcttgt gatcgcgcgc cggaggcaag acccatatgt cagcgccgtc cgacaaacac
     3721 attacgctgg cctccggccc gacaagcctc tcctccacga gtatcttgca gccccgcccc
     3781 gcgcccatag cgccaagctc gtccactgcc cgcccggcct cctccgggct atcggcaact
     3841 accacgccct tgcccgccgc gaggcagtcc agcttgacca cgacgccgcc cgcctcctgc
     3901 gcaatagtcc gggcagcatc acgcgcgcgg ccaacatctg tgaatgcctc gcagtgcgca
     3961 gttggtattc cggctcgggc catgacgccc ttggcatagt gcttggacga ttcgatccgg
     4021 gccgcagctg cgctcggccc gaataccgtt acgcctatgg cgcgcatggc atcggcgagc
     4081 ccggctgcca gaggcgcctc agggcctacc acgaccaggc tgcactcggc ctcccgggcc
     4141 aacctgacca gcccggccac gtcggtcgcc ccaaccggcg cgcacgcggc gagtcggccg
     4201 attccggggt tacccggagc agcgataacc cgtcctatcc tggggcttct tgccagtttc
     4261 cagactatcg cgtgttccct gcctcccgac cccaccacca gcacattcag gcgagaagac
     4321 gaatcaggcg ttctcatatg tacgcctcct cagtgtcgga agtgccgtct gcctgtgaag
     4381 accattgcga tcccgtgcgc atccgcgacc tggaccgatt ccttgtctcg aagggatccg
     4441 ccaggctgga ttatggctgt cacgcccgct ttcgccgcaa cctcgacggt gtcgggaaac
     4501 gggaagaaag cgtctgaagc catcactgat ccctctgccc gctgccctgc ctgttccata
     4561 gctatccttg cagagcccac ccggttcatc tgccctgcgc ccacacccac ggtaccgttt
     4621 cctcgccaca gcactatggc gttggacttc acatgggcca ctgccttcca tgcgttcgcc
     4681 agttgaacca tctcgccttc gtccggcgcg cgcctggtaa ctaccggtaa tccccgcatg
     4741 tcaaggtgtc caaccctatc aggctcctgc accaagaacc cgccagcgac agaccgcacg
     4801 tcccacccgg ttccagccgt gatatcatcc actgccagct ctagaagacg caggtttttc
     4861 ttgccatgaa gcagctccac ggcttccgga acaaatcccg gcgctattat tacctcgagg
     4921 aatatctccg acatcagccg cgcagtctcc aggtccacag ggcgattgaa ggctgcgatt
     4981 ccgccgaacg cggatactgg gtcggaatcc agcgccgccc gatatgcctc cgccacattc
     5041 gcgcctaccc ctatgccgca cgggttagcg tgcttgacga tgaccactgc ggtctcgtcg
     5101 aactcagcca ccgctctgac agcagcgtcc gcgtctccga tgttattgaa ggacagctcc
     5161 ttgccaccca gctgtttcgc tgcggccagc ccccacgagc gctcgcccac actgcgatag
     5221 aaagcagcca tctgatgcgg gttttcgccg tagcgaaggg tctggatcat tacgcctccc
     5281 agggtaaact cgcgccccag accctgacct gcctcccgct ccccgccagc caatcctgtc
     5341 tggcctgcct ctcgcccctc gtccgcctca cgctgctcac actgcctggg cacgccggcc
     5401 atgctatcgg cgaagtaacg ggcaatggca gcgtcatacg ccgccgtatg ctgaaaagcc
     5461 gccgccgcca ggcgcactcg agtacttccc gataccgacc cgttctctct cagctcagcg
     5521 acgacaacgg ggtaatctgc cggatcgacg atgaccgcta ccctctcgaa gttctttgcc
     5581 gccgcccttg ccatggacgg accgccgata tctatgttct ccacgatctc cgcctcgtcg
     5641 gccccgctat ccaccgtgtg ctggaatggg tagaggttga ccgcgactat gtcgatggga
     5701 accacatcga gctcctccag agcctgcata tgctctggag taggcctggc caggatgccg
     5761 gcatgaacac gcggatgcaa ggtcttgacc ctaccgtcaa gcacttcggg aaaacccgtc
     5821 acctgagaaa cgtctgttac acccagcccc gccctacgca gagccgaggc ggtcccgcca
     5881 gttgagatta tctcgaagcc caggtccgcc agggcccgcc cgaattcaac cacgccttcc
     5941 ttgtcgtaca cactcatgag agctctacgc ctgttcctgc ccacggcgcg cacatctccc
     6001 ttcgcctatc ttcctgcctg ttttcccgcc cgttttgccg cccacctgct cgcccacacg
     6061 cgcgttcaca cgctcgttag catgctcgtt agcatgctcg ttcatctgct ggcccagacg
     6121 ccctatgacg tccaccagca attcgtgctc ctgctccagg atcctcgccg acaggctgtc
     6181 cacactgtcg cccggcatca cttccaccac gcgctggtcg atgatggcac ccgagtccat
     6241 gccgtagtct acataatgca cagtgcaccc tgagaacctc actccgtatt ccagcgcttg
     6301 tctttgagcc tcaagccctg gaaaagccgg caacagagac ggatggatgt tgatcacacg
     6361 gcccggaaac cggtccagaa acgccggacc cagcaacctc atgaaacctg ccaacactac
     6421 gaggtccggc cgggccttct cggccgcccc ggccaaagcc agttcgaact cggcccgcga
     6481 ggagtagtcc cgtcgccgca caacaaccgc tggaatccct acctccagcg cccgggccac
     6541 ggcaggcgcg tcggcagcgt cggaaaccac gatctcggcg gacgcctgca gccgtcccga
     6601 ccggatggca tctattatcg cctgcagatt cgaaccccgg cccgacacta gcactgccaa
     6661 gcggatactc acgatgtcac gccccctcga acagcacttt cccattgccc gggactatat
     6721 cgcctatgac gtaggcattc tccccattgg cctgcaagat ctccactgcc tgttgcgcgt
     6781 cgccaggccg aaccacaacc atgaacccta tgcccatgtt aaacacccgg cgcatctcgc
     6841 cagcgtccac gttgccgatc tcctgaatca tccggaatat gggatgcacc ggccattggc
     6901 cccactctag ccgcagcgat acgcctgtgg gcaacattcg aggcgggttc tcgacaagcc
     6961 cgcctccggt gatgtgcgcc attcccagca cacgcagttg ctccatgagt gcaagcaccg
     7021 gccgcacata tatcctggtc ggcctgagca tctcgtcgcc cagcgtgcag cctgaggcga
     7081 agccgattgt gccagcggat tccgatcctc gtcgcgtccc cagcccatcg cagctttgcc
     7141 ccacggtcaa gccgccctgt tccaacaggg cctttcgggc gagagagtat ccgttagaat
     7201 gcagcccgga cgaggccagg ccgatcaccg catccccggg ccggatatct gagccgtcga
     7261 ctactttccg cctgtccact gctccaactg cgaaccccgc gatgtcgtat tctccgtccg
     7321 ggtagaatcc gggcatctcg gcggtctcc
//
LOCUS       DAOTAC010000107         7313 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_8642_length_7313_cov_5.294847, whole genome shotgun sequence.
ACCESSION   DAOTAC010000107 DAOTAC010000000
VERSION     DAOTAC010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7313
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8642_length_7313_cov_5.294847"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="PLM74_05845"
     CDS             <1..846
                     /locus_tag="PLM74_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HON42641.1"
                     /translation="RKPYEPGEAVCSEEMLWCGSCRPCADGYPNQCEALHELGFSCDG
                     AFAEYIVVDARYLWSIEELKEKYPGDDAFVAGALVEPTSVAYTAVIDTAGGIRPGDNV
                     VVCGGGPIGLAAVAVLKRAGAAKVILSEPAQNRAELGRKMGADFVINPLKENFAEAVL
                     EITRGVGARLFLEATGLPAAVWPGIEQVIWEGKTIDAVVSIVARADAKIPLTGEIFQV
                     RRARIAGAQGHSGHGTFPRVISSIAAGMDVLPLVTKRITMDEVPENIIMLQTDRSECK
                     ITCMM"
     gene            863..1891
                     /locus_tag="PLM74_05850"
     CDS             863..1891
                     /locus_tag="PLM74_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HON42642.1"
                     /translation="MRDKTMLAAVFEDHGKLSVKEVPVPAIREPDEVLLRVDAASICG
                     TDVHILEVPPGHPATPGAILGHEYTGTVLEMGSGVQGLKTGDSVVVDPNLTCAKCRYC
                     RSGMPNMCQSMTTLGIFTNGGFAKYNVAPASALHKISTDVPPDVAALAEPLSCVVNGT
                     QKVRVQPGESVAVLGAGPIGLLFTQVFAAAGAGRIVVSEITKVRARAAAKSGAHVVLD
                     PREPGFVQRVLDETGGGADVVVDAVGTLLPTALDLVRRGGKVLLFGMNQQATPPVPQN
                     TITRYEISVLGTYIAKYTFPPAIRVLESGAINADALITHRVNIANIQEGLDAMRYGEA
                     VKVIVSPE"
     gene            1958..2929
                     /gene="iolN"
                     /locus_tag="PLM74_05855"
     CDS             1958..2929
                     /gene="iolN"
                     /locus_tag="PLM74_05855"
                     /EC_number="3.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003542277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-scyllo-inosose hydrolase"
                     /protein_id="HON42643.1"
                     /translation="MSKWQLPPRKGHMDEPSGVYLQNMTMKQIQERLTKDDIIIVPVG
                     STENHGPNACIGEDTFLVTRMAEAVAIRTGCTVAQPVWYGSHPFHHLGMPGTVVVPEE
                     VFCGLLKSIMAGLWNMGFRKMILLNGHGQEYVIPTAIHQFGKTYQVPAVIINLNWYHA
                     VQDHFKLKSEGGVYETPFIHADEVETSWSLALFPELMDQSAAVDTVPRGYMPEGHVDK
                     AGCLLHTPIAWYGHIGCGPAEYAATPEGCVGKATLADPEKAMPGVEAVLDYMVKLHDD
                     ILAVFPVGQLPPIEDMSQRKREDIEAVLRGPLAPGGRSIYTLVYPPA"
     gene            2945..3787
                     /locus_tag="PLM74_05860"
     CDS             2945..3787
                     /locus_tag="PLM74_05860"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HON42644.1"
                     /translation="MKISIVVNATPRATGLAADLPLEDAVALAAKVGYDGVELAVGDP
                     RDLDVGTVRQTLSMYGMGVPAIGTGAAYRQGLSLAHADGSRRAGAIERLKDHVDVGAE
                     LGAAVIVGLIRGRTADGLPPREGLARISEGLQEVAGYAARQGVRLFLEPINRYETDLL
                     NTVSDALTTAREAGENVGLLVDTFHMNIEDSSIARAIRDAAPRIWHVHVADSNRRAPG
                     AGHIDFGEVIEALKDIGYQGYVSGEMIMEPDAPAAFSALYSHLAPMIARYICEAGADH
                     SGGR"
     gene            3860..4825
                     /locus_tag="PLM74_05865"
     CDS             3860..4825
                     /locus_tag="PLM74_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013932388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HON42645.1"
                     /translation="MKRVFGLCLIACLAIILAGPSPAASGADQMTFYVIAHTGPGDPF
                     WAVVQRGVQDAGKALGVKAVFQGPAGYNVPEQVNMFNAAMAAKADGIATSISDARAWA
                     EPIREARKRGIPVVAINCQEPEEFRGTIPYMSYIGMDEYEAGKTVARRMLPKLKQGAK
                     VVVAIHQAGHVGLEARAKGIGEVIAKDLGGKTDKLDITQDATQAIGILRSYLKANPDT
                     AAIFTVGPLGAIPAIKMIRDDGLKGKVLMASYDLEPVTIQAIKDGICEGTMDQQQYLQ
                     GYMAVVELYLNAKFQLDPADYDTGRGFVTPENVDAVAALVKAGYR"
     gene            4956..5912
                     /locus_tag="PLM74_05870"
     CDS             4956..5912
                     /locus_tag="PLM74_05870"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HON42646.1"
                     /translation="MRPLLNRREVGVAVALVLVFIVFFSMKREFLNAGTLGDIVTVAA
                     ELGVVAIGVSFLMIAGEMDLSVGSNFALSGMVLAMLITKSGWNPWAALLAALACGAGI
                     GAVNGLVTLYARIPSFITTLGAMMFWRGIALYLSQGWPISIFEDVPLLRLLGGARVLS
                     TAHISAVWWFVIGLVFCLVLEKTAYGNWVFATGGKRAAARALGVADTKVKMTNFIITG
                     VLAALSGCLQLGRMGSMSPVWGQELSLEAIAAAVVGGNSLSGGIGTIFGAMLGAVTMA
                     SIRIGLVMVGAPSYWYMSFVGFIVVAAVALNVKLGEVISWRK"
     gene            5900..6682
                     /locus_tag="PLM74_05875"
     CDS             5900..6682
                     /locus_tag="PLM74_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013932390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HON42647.1"
                     /translation="MEEVSCRARQDPLMEAIGLCKRFGRVVALEDVDVTIQSGEVVGL
                     LGDNGAGKSTLIKILTGVYRPDGGELKFEGKPVDFASPKDARRLGIETVYQDLALIEN
                     MSIARNFFLGAEPSRRIGGFNLLDMAAMRRVTEEALRDIGIAVRCATDDVCVLSGGER
                     QAIAIGRTMYFGGKLLILDEPTSALSVHETNKVLGYITEARKRGLAVIFITHNLYHVY
                     PVADRFIVLEHGRKVADLAKDEATVDDLIRIVAYGIEGTRRD"
     gene            6831..>7313
                     /locus_tag="PLM74_05880"
     CDS             6831..>7313
                     /locus_tag="PLM74_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42648.1"
                     /translation="MDVGRPMANALSQAIRFLSRQPRDWKVTACRASVDKLLYQMVFP
                     YLSVYIALLGATGTQLGVVNSVGMAAGAVLGLASAAAANRLGTKRVYVIGVALVGLSY
                     LVTGLASSWVVALMGTMIWWCGSTVSGHTCGIVCGSSLLNKDRATAMGCCESLAQGVM
                     G"
BASE COUNT         1483 a         1934 c         2433 g         1463 t
ORIGIN      
        1 cgcaagccgt atgaacctgg cgaagcggtc tgctcagagg agatgctatg gtgcgggtcg
       61 tgtcgcccat gcgccgacgg ctaccccaac caatgcgaag ccttgcatga actcggtttc
      121 agctgcgacg gcgcattcgc tgagtacatc gttgtggatg ccagatatct atggagcatc
      181 gaggagctta aggagaaata cccaggcgac gacgcctttg tcgcgggcgc gcttgtggag
      241 cccacgtcag ttgcgtatac cgcagtcatc gataccgcag gcgggattcg tcctggcgac
      301 aacgtggtcg tttgcggagg cggtcccatt gggctggctg cggtcgccgt acttaagcgt
      361 gccggagctg ctaaggtcat cctgtcggag cctgctcaga accgtgccga gcttggccgc
      421 aagatgggcg ccgacttcgt gatcaaccca ctcaaggaga acttcgccga agcggtgctc
      481 gagataaccc ggggagtagg cgcccgcctg ttccttgagg caacaggcct gcctgcagcg
      541 gtttggccag gcattgagca ggtcatctgg gaaggcaaga ccatagatgc ggtagtatcc
      601 atcgtagccc gagcggatgc gaagatcccg ctcaccggag agatcttcca ggtgaggcgt
      661 gcccggatag ccggggcgca ggggcactcc ggtcacggta ctttcccccg ggtcattagc
      721 tctatcgctg ccggtatgga tgtcctgccg cttgtgacca agaggatcac tatggacgag
      781 gtgcctgaga acatcatcat gctccagacc gacaggagcg agtgcaagat cacttgcatg
      841 atgtagtgcg ggagggggac atatgcgcga taaaaccatg ctggctgcgg tgtttgaaga
      901 ccatggcaag ctctcggtga aggaagtgcc ggtcccagct atccgggagc cggatgaggt
      961 cctgctcagg gtggatgccg ccagcatctg cgggactgat gttcacatcc tggaggtgcc
     1021 gcctggccac ccggcaaccc ctggggctat tcttgggcac gaatacacag ggactgtcct
     1081 tgagatgggc tcgggcgttc agggacttaa gaccggcgat agcgtggtgg tcgaccccaa
     1141 cctgacgtgc gccaagtgcc ggtactgccg ctcggggatg ccgaacatgt gtcagagcat
     1201 gacgacgctt ggcatcttca caaatggagg gttcgccaag tacaacgtgg caccggcaag
     1261 cgctctgcac aagatctcga cggacgtacc gccggatgtg gcggcgcttg cggagcctct
     1321 ttcgtgcgtc gtgaacggca cgcagaaggt gcgggtgcag ccgggagagt cagtggcggt
     1381 gctgggcgcg ggccccatag gtctgctgtt cacccaggta tttgcggctg caggcgctgg
     1441 ccggatagtc gtttccgaga tcacgaaggt cagggccagg gcggctgcaa agagcggggc
     1501 gcatgttgtg cttgatccgc gcgagccggg gtttgtgcag cgcgtgctgg acgagacggg
     1561 gggcggcgcg gacgtggttg ttgacgctgt ggggacactg cttcccactg cgctggatct
     1621 ggtacgtaga ggcgggaagg tgctcttgtt cggcatgaac cagcaggcta ccccgccggt
     1681 gcctcagaac acgatcacgc gttatgagat atccgttctc ggtacgtata ttgcgaaata
     1741 cacatttccg cctgctatcc gcgtgcttga aagcggagcc atcaacgccg acgcgctgat
     1801 cacgcatcgt gtgaatattg ccaatatcca agaaggactc gacgcaatga gatatgggga
     1861 ggccgttaag gtcatagtca gtcccgagta gcctgcccgg gttgcctggc agggatctca
     1921 tagacgcgaa atgctgaagg tgaaggggag agtccaaatg agcaagtggc aacttccgcc
     1981 ccgaaagggg cacatggatg agccttccgg ggtgtatctg cagaacatga cgatgaagca
     2041 gatccaggag cggctcacaa aagacgacat tataatcgtg ccggtggggt cgaccgaaaa
     2101 ccatggcccg aatgcgtgca taggcgaaga tacattcctg gttacgagga tggcagaggc
     2161 cgtggcgatc aggaccgggt gcactgttgc gcagccggtg tggtatgggt cgcatccatt
     2221 ccatcacctg gggatgcccg gcacagtggt cgtgcccgag gaggtcttct gcggcctgct
     2281 caaatcgatc atggcaggcc tgtggaatat gggcttccgc aagatgatcc tcctcaatgg
     2341 gcatgggcag gagtacgtaa tccctacagc cattcatcag ttcggaaaga cgtaccaggt
     2401 gcccgcggtc ataatcaacc tcaactggta ccatgcggtg caggaccact tcaagctgaa
     2461 gtcggagggc ggagtgtatg agaccccgtt catacacgcc gacgaagtgg agacctcctg
     2521 gtcgttggcg ctcttccctg agctgatgga ccagtcggct gcggtcgaca cggtacctcg
     2581 cggatacatg cccgaaggcc atgtcgacaa ggcaggttgc ctactacata caccgatcgc
     2641 gtggtatggc cacatcggat gcggacccgc ggagtacgca gccacgcctg aagggtgtgt
     2701 gggcaaggct acactggccg atcctgagaa ggcgatgcct ggggtcgagg ccgtgctgga
     2761 ctatatggtg aagctgcacg acgacatcct ggccgtcttc cctgtcggcc agctgcctcc
     2821 catcgaagat atgagccagc gcaagaggga ggacatcgag gcggttctca ggggacctct
     2881 ggcccctgga gggagaagca tctacaccct agtgtatccg cccgcttgac gggggctatg
     2941 cggaatgaag atcagcattg tagtaaacgc cactccccgc gctacggggc tcgccgccga
     3001 tctgccgctt gaggacgcgg tggcgcttgc cgccaaggta ggttacgacg gggttgagct
     3061 tgccgtgggc gacccgcgcg acctggatgt cggcacggtc cgccaaacgc tgagcatgta
     3121 tgggatgggc gtcccggcca taggaaccgg cgctgcttac cggcaagggc tgagcctggc
     3181 tcacgcggac gggtcgcggc gggcgggggc gattgagcgg ctgaaggatc atgtagatgt
     3241 cggtgccgag ctcggcgcgg cggtgatcgt tggcctgatc cggggtagaa ccgccgacgg
     3301 tcttcctcct cgcgagggtc ttgcacggat aagtgaaggt cttcaagaag tcgcaggata
     3361 cgccgccagg caaggcgttc gactgtttct tgagccgatc aacaggtacg agactgatct
     3421 gcttaacacg gtcagcgatg cgctgactac ggcacgcgag gccggcgaga atgttggcct
     3481 cctagtcgac acattccaca tgaacataga ggattcgtca atagccaggg ctatcaggga
     3541 tgctgcgcct cggatctggc atgttcatgt ggccgacagc aataggcggg cgccgggcgc
     3601 cgggcatatc gactttggcg aggtgatcga ggccctgaag gatatcggat accagggcta
     3661 cgtgtctgga gagatgatca tggagccgga cgctccggcg gcattctccg cgctctactc
     3721 tcatttggcg cccatgatag cgcggtacat atgtgaggct ggagcggatc acagcggtgg
     3781 gaggtgagcg atgagatagc tgtccttgga tggcattaag ccacagtcta ctgtcagacc
     3841 attatgagga ggggtacaca tgaagagagt cttcggattg tgcctgattg cctgcctggc
     3901 gattatacta gccggcccga gccctgcggc gagcggtgcc gatcagatga ccttctatgt
     3961 gattgcgcac acaggtcctg gcgacccgtt ctgggcagtg gtgcagcgtg gcgtgcagga
     4021 tgccggcaaa gccctgggcg tcaaggcagt tttccagggg ccggcaggct acaacgtgcc
     4081 tgagcaggtg aacatgttca atgcagcaat ggcggctaag gcagacggca tagctaccag
     4141 catcagcgat gcgcgggcct gggctgagcc tatccgcgaa gctaggaagc gtggcattcc
     4201 ggtggtggct atcaactgcc aagagcctga ggagttcagg ggtactatcc cgtacatgtc
     4261 atacatagga atggacgaat acgaggcagg caagacggtg gcgcgccgga tgctccccaa
     4321 gctgaaacag ggggcgaagg tcgtcgtggc aatccaccag gcagggcatg tgggccttga
     4381 ggctagggca aaagggatcg gagaggtgat agccaaggat ctgggcggca agaccgacaa
     4441 gctcgacata acccaagacg ccacacaggc aatcgggatt ctcaggagtt acctcaaggc
     4501 gaatccagac actgcggcga tattcacagt gggtccgctc ggcgccatac ccgccatcaa
     4561 gatgatcaga gacgatggcc ttaagggcaa ggtcctcatg gcctcgtacg acctggaacc
     4621 agttaccatc caggccatta aggacgggat ctgtgagggc accatggacc aacagcagta
     4681 tctccagggc tacatggccg tagtcgagct ttatctgaat gccaagttcc agctcgaccc
     4741 ggccgactac gataccggac gcggtttcgt gacgccggag aatgtcgacg cggtggccgc
     4801 gcttgtcaag gcggggtaca gatagcctgc gacccggggc acgtcagcgc gaatgagcag
     4861 gctgggctag ccccgacatg caagtcagca cgaacagccg aaggcgcggg gattcccccc
     4921 gcgcccggct ggatgaaggg gtggcgattt gaccgatgag gcctcttctg aaccgccgcg
     4981 aagttggtgt ggcagtggcg ctggttctgg tgttcatcgt gttcttctcg atgaaacgtg
     5041 agttcctgaa tgcaggtact ctgggcgata tagtgaccgt cgctgcggag ctgggcgttg
     5101 tggctatagg tgtgagcttc ttgatgatcg ccggtgagat ggacctatct gtgggttcga
     5161 acttcgccct gagcgggatg gtccttgcca tgttgatcac taagtccggc tggaatccat
     5221 gggctgcact ccttgcagcg ctggcctgcg gcgccggtat cggcgcggta aacgggctgg
     5281 ttacgcttta cgcccgtatt ccatcattca ttacgactct gggcgccatg atgttctgga
     5341 gagggattgc gctctacctg tcgcagggat ggccgatcag catcttcgaa gacgtgcccc
     5401 tgctacggtt gctcggcggc gccagggtct tgtctacggc ccacatctct gcagtatggt
     5461 ggtttgttat cggcctagtt ttctgtcttg tactagagaa gacagcctac gggaactggg
     5521 tgttcgcgac gggcggcaag agagctgctg cgcgtgcgct aggcgtggcc gacaccaaag
     5581 tgaagatgac caacttcatc atcacaggcg tgctagccgc cctatcgggt tgcctgcagc
     5641 tgggaagaat gggttccatg tcgccggtgt gggggcagga gttgtccctc gaggcgattg
     5701 cggctgctgt ggttggcggt aactcgctct cgggcggaat cggaacgatc ttcggcgcga
     5761 tgctgggcgc ggtcacgatg gcgtccatca gaatcgggct cgtaatggtg ggagcgcctt
     5821 catactggta catgtcattc gtggggttca tcgtagttgc agccgtcgcg ctcaatgtca
     5881 aactcgggga ggtgatatcg tggaggaagt gagctgccgg gcccgccagg accccctcat
     5941 ggaggcgatt gggctatgta agcggttcgg acgcgtggtc gctctcgaag acgtggacgt
     6001 aacgatccaa agcggcgagg tagtcggact tctgggcgat aatggcgccg ggaagtccac
     6061 cctaataaag atcctcacgg gggtataccg gcccgacggg ggcgagctga agttcgaggg
     6121 taagccagtg gatttcgcat ctcctaaaga tgcgaggcgc ctcggaatcg agaccgtcta
     6181 tcaggacctt gctctcatcg agaacatgag catcgcccgg aatttcttcc ttggagccga
     6241 gccctcccgg agaatcggag ggttcaacct gctggacatg gcagcgatga ggcgcgtgac
     6301 cgaggaggca cttcgagaca tcggcatcgc cgtaaggtgc gcaaccgatg atgtatgcgt
     6361 cctgtcgggc ggggagaggc aggccatagc catcgggcga acgatgtact tcggagggaa
     6421 gctgctgatc ctggatgaac ccacttcggc tctttcagtt cacgagacca ataaggtgct
     6481 cggctacatc acagaggcca gaaaacgcgg cctggcagtg atattcatca cccacaacct
     6541 atatcacgtc taccccgtcg ccgataggtt catagtgctc gaacacggcc gcaaagtcgc
     6601 ggacctggcc aaggatgaag caaccgtaga tgatcttatc aggatcgtcg cctacggcat
     6661 agagggcacg aggcgagatt aatgcatacg gctgacgggg ccagtcctat ggtagtattc
     6721 atagtaccgg ctcttttcga atgtactctt gggctgacgc ttactgtgcc cagggcatgg
     6781 tcctcaaact gcgatagtgc agggccggca gtcgcagggt tgggatggga atggatgtgg
     6841 gcagaccgat ggccaacgcg ttgtcgcagg cgattcgctt cttgtctagg caaccccgtg
     6901 attggaaggt tacagcgtgc agagcgtctg tagacaaact gctctatcag atggttttcc
     6961 cgtacctgag tgtatacata gcgcttctcg gggctacagg aacacagctg ggtgtagtca
     7021 acagtgtcgg tatggctgca ggcgcagtgc ttgggttggc gtcagcggcc gctgccaacc
     7081 ggctgggaac caagcgagtg tacgtgatcg gtgtggctct ggtggggctt tcgtatctag
     7141 ttactgggtt ggctagtagc tgggttgttg cgctcatggg taccatgatc tggtggtgcg
     7201 gatcgacagt atccggtcac acctgtggga tcgtgtgcgg gagttcgctt ttgaacaaag
     7261 acagggcaac tgcgatgggt tgttgtgaga gccttgcgca gggagtgatg ggg
//
LOCUS       DAOTAC010000108         7299 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_8660_length_7299_cov_4.635975, whole genome shotgun sequence.
ACCESSION   DAOTAC010000108 DAOTAC010000000
VERSION     DAOTAC010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7299
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8660_length_7299_cov_4.635975"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="PLM74_05885"
     CDS             <1..325
                     /locus_tag="PLM74_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42649.1"
                     /translation="DPRMFNTVMPFPTVSADSPRDSPEKYWRGPVWLDQAYFAVEGLA
                     RYGFVDDARHMARRLLHAAEGLAGNGPIRENYNPETGAGLNAANFSWSAACFYLLITE
                     CIGRK"
     gene            complement(353..1081)
                     /locus_tag="PLM74_05890"
     CDS             complement(353..1081)
                     /locus_tag="PLM74_05890"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HON42650.1"
                     /translation="MKAVWAIYRANFREYARNPAVLAFTVIMPLLLAIFLSLIMGNQD
                     LFTIDYYLPNMLGISLMWLGVFATAQPLVEQREQKIFRRMAVTPLSRQSMLWGQVLFR
                     VTVGLFQAAVFLIAGRIGFGVRVQGSWLLLLGATVMGALVFVTLGYVLAGLARSQEAA
                     PVIAQPVQMGMMFLSSTLLPAAMLPSGVRAVVRAIPLTYVADALRQTISRVPPMLPLW
                     VDFAAMAGWILLFFVLSVRLFKWE"
     gene            complement(1078..1995)
                     /locus_tag="PLM74_05895"
     CDS             complement(1078..1995)
                     /locus_tag="PLM74_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON42651.1"
                     /translation="MDSLNWAVWVKDLTKRYGDVRAVDGISFGVSRGEVFGMLGPNGA
                     GKTTTIEILLGLRTADSGDVRVLGMTHAENHRTIACAIGAQLQTTGMYPTHTVRETIQ
                     LFSDFYPEAAPLDELIDMVGLREKAGALVKNLSGGQRQRLAIAIALVNRPHVVFLDEP
                     TTGLDPQARRALWDMILSLKASGTTVLLTTHYMEEAEQLCDRVAIVDRGKIVDSPAAL
                     INKHFKHIAIEFEAPPGAEVDAHDLRSLPGVANVVVDGGKVTLYSSDVPATMASLLGA
                     ASHSLRHMVVRQATLEDVFLKITGRSMRQ"
     gene            2218..2391
                     /locus_tag="PLM74_05900"
     CDS             2218..2391
                     /locus_tag="PLM74_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42652.1"
                     /translation="MIIIMGSVKIEVSRVRDHMWGDGAQRTHEQNTLERAIREARLAQ
                     EVTAASYLSGLLR"
     gene            2539..3366
                     /locus_tag="PLM74_05905"
     CDS             2539..3366
                     /locus_tag="PLM74_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HON42653.1"
                     /translation="MYSRALALIFALSLVIGISGCNGESASVHGVVEGLVTDQVSGRP
                     VRDVWVTTGSIVAWTDALGAYWLSLPQGTQSICFQRAGYARKTLPVTVVGRSTVQTNL
                     EMNPILPAGPVEISLTWGASPVDLDAHLWTSGGYRINHSCGGSLTSPPWAKLDYDYFA
                     GHGRETITIADFAPDTYHYSIHNCSRTPAIAGSGAQVDVYSQSQLLYTFIVPHTGPAD
                     YLVWDVFEIDGATREMARLDTLRECPNWTLEQIGPVDKPEALNSAGFRPVDESPTEQ"
     gene            complement(3476..4108)
                     /locus_tag="PLM74_05910"
     CDS             complement(3476..4108)
                     /locus_tag="PLM74_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HON42654.1"
                     /translation="MPNVIPFLSYVLIVTFTPGPNNIMSLVMGSKYGFARTLPFRLGV
                     AVGFGLVMIVSSYLNLFLFTVVPKMKPIAETVGGAYMLYLAARVLMSKVDATNDDAHL
                     TSFPAGVVLQFINPKAILYGLTVTATFITPYYHSPVALLFFSAFLGSISLASTSCWAM
                     FGTGIQRLLGRYGRPFNIAMAMLLVYTAASVSGMLPRIQTLIRAALSRGV"
     gene            4320..5450
                     /locus_tag="PLM74_05915"
     CDS             4320..5450
                     /locus_tag="PLM74_05915"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HON42655.1"
                     /translation="MNASRIVTLQRALRDQGLACAFLVPGPNLKYLTGLQLEAFERVI
                     LGIVPPYGDPAFIVPQLEADKVRGHVGTHLHCDTVFPYTDDAGPLDALRQALRTLGLG
                     PDRGPAAVGAEFLHMRLLERQVIEQASGHIESCHSMQFRDLGPITARMRSVKDPDELA
                     ALKRAARIVDAGIAAAASAIAPGVTEREVAAAAERAMAAAGADSIPFNAVLAGPNSAL
                     PHGQTSDYAMRDGDMVICDIGATYRGYNGDITRTFAVGKPTSQMARIHSIVYDANRAA
                     CQAARPKVTGEQLDWIARDVITRAGFGGYFTHRTGHGLGLEIHEEPYIVQGATEPLKA
                     GMVFTIEPGIYIPGVGGVRIEDDLALTDDGCMVLTASPRELA"
     gene            complement(5457..6023)
                     /locus_tag="PLM74_05920"
     CDS             complement(5457..6023)
                     /locus_tag="PLM74_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42656.1"
                     /translation="MTKRRAFVHMLIALLAMAMVCSAGSTVADAKKPAKVKAPKPPKE
                     RKIDAAVTKRPTQLRTESGLVVMNLPEGVTFAAAEHKGKAVWVYGPSGQRYWLPIQDV
                     KMIKGHKVSVDDVSDKQLKSFGLQVTAARVTAVMQSVYPTPGTTLTAGMRTEWQDKVL
                     IEMAIKAEITLPQLSLMLEWAVFSGYVD"
     gene            complement(6125..6991)
                     /locus_tag="PLM74_05925"
     CDS             complement(6125..6991)
                     /locus_tag="PLM74_05925"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HON42657.1"
                     /translation="MRGCVFRVCRLVTGLLMVAALCATAPVHAGKAITHVVGAARPLV
                     IAHRGASGVAPENTMASFEQAIRFGADMIELDVHGSKDGYLIVIHDASTSRTAKGNCQ
                     GAVANLTLVQLKALDVGVWKDSGFAGERMPTLDEVLAAFKERALFLIELKARGIEERV
                     AQVIHEAGMEGRVMLQSFDAESVRIMRGLLPETPAGILYRDTWVLDPAGRGRRIVEQV
                     LEVGASFAGMNVGAVSAELVKVLNSSGIDVFAWTADDDWVFTHLIQAGVDGIITNYPE
                     RLLRHLGRRVGE"
     gene            7120..>7299
                     /locus_tag="PLM74_05930"
     CDS             7120..>7299
                     /locus_tag="PLM74_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42658.1"
                     /translation="MSQMCAKDEYCWEDLSLGEAEVTQALAGLRASQPGRPIRGIAQP
                     GHAPEPAEEEVVDPDN"
BASE COUNT         1612 a         2303 c         1954 g         1430 t
ORIGIN      
        1 agatccacgc atgttcaaca ctgtcatgcc gtttcccaca gtgtcggctg atagccccag
       61 agactcgcct gagaagtact ggagaggccc cgtatggctt gaccaggcgt atttcgccgt
      121 ggaaggcctc gcgagatatg ggttcgtcga cgacgctcgg cacatggccc ggagactgct
      181 acacgctgcc gaaggtctcg caggcaacgg tccgatcagg gaaaactaca accccgaaac
      241 aggcgccgga cttaacgcgg ccaacttcag ctggtccgcc gcatgcttct atctcctgat
      301 caccgagtgc ataggtcgaa agtgatacct gttggaggtt gctcgcagtc gtctactccc
      361 acttgaacag cctcacagag agcacaaaga acagaagtat ccatcctgcc atggcagcga
      421 agtctaccca cagagggagc atgggaggca ctctggagat ggtctgcctt agcgcgtcgg
      481 ccacgtaagt cagaggaatc gcccgcacca ctgcacgcac gccagacggc agcatcgccg
      541 ccggcaggag agtgctcgac agaaacatca tccccatctg cacaggttgc gcaataacgg
      601 gggccgcctc ttggcttcgg gcaagcccgg caagtacata tcccagtgtc acaaagacca
      661 gggcgcccat gactgtcgca cccaacaaaa gcaaccagct tccctgcaca cgaactccga
      721 atccgattct ccccgcaatc aggaagaccg ctgcttgaaa caggcccaca gtcacgcgga
      781 ataggacctg gccccatagc attgattgcc gagacagagg agtcacggcc atccgcctga
      841 agatcttctg ttctcgctgc tctacgaggg gttgagcagt ggcgaacact cccaaccaca
      901 tcaatgagat acccagcata ttagggagat agtagtcgat cgtgaaaaga tcctggttgc
      961 ccatgatgag gctgagaaag atcgccagca gcagcggcat gatgactgtg aaggccaaca
     1021 ccgccggatt cctggcgtat tccctgaagt tcgcccggta gatcgcccac actgccttca
     1081 ttgccgcatc gacctccccg tgatcttgag aaacacgtct tcaagcgtgg cctgacgcac
     1141 aaccatgtgc ctaaggctat gcgaagcagc accaagcagg gacgccattg tggccggaac
     1201 gtcagacgag tatagcgtca cctttccccc gtcaacaacg acattcgcca ctcccggcag
     1261 agaccgcaga tcatgtgcat cgacctctgc accggggggc gcctcgaact ctatcgctat
     1321 gtgcttgaaa tgcttattga tcagcgctgc cggagaatcc acaatcttgc ctcggtcgac
     1381 tatggcgacc ctatcgcaca gctgctctgc ctcctccatg tagtgagtcg taagcagcac
     1441 tgttgtgccc gaagccttca gagagaggat catatcccac aatgcgcggc gagcttgtgg
     1501 gtccaggccg gtggtgggtt cgtccaggaa gactacgtga ggccggttca caagcgcaat
     1561 ggcgatcgcg aggcgctgtc gctgcccgcc ggacaagttc tttactagtg cccccgcttt
     1621 ctcacgcagg cctaccatgt ctatcagttc gtccaacggg gctgcctcgg ggtagaagtc
     1681 cgaaaacaac tggatcgtct cgcgcacggt atgcgttggg tacatcccgg ttgtctgaag
     1741 ctgcgcacct atcgcacaag cgatcgtcct gtgattctct gcatgcgtca tcccgagtac
     1801 acgcacgtcc cccgaatctg cagtacgaag accaagcagg atttcgatgg tagtggtctt
     1861 gcctgcccca ttcggcccca gcatgccaaa gacctcgccg cgcgatacgc cgaaggagat
     1921 tccgtccact gcccgtacgt caccgtaacg cttcgtcaag tcctttaccc agaccgccca
     1981 gttcagcgag tccaacacgc cacctccctc tcccatattc actgtcaatt gtgattcgat
     2041 gaactgctag ggcatccctt ctttgcttgt caaccgctcc ggcgtgaccc gcttctccga
     2101 aatcaactat gatgttgaca tcatgaatga tatgctttat gatattgatg ctgaggttaa
     2161 taatcttgag ttcacacgcg atgccagttc cataacacca actaggaggg gtgggccatg
     2221 atcatcatca tgggaagcgt gaagatagag gtcagccggg tacgggatca catgtggggc
     2281 gatggcgcgc agcgcaccca tgaacagaac accctggagc gggcaatccg ggaggcgcgg
     2341 cttgcccaag aggtcacggc cgcctcgtac cttagtgggc tgctgaggta gggcgaataa
     2401 cccccgaagg catcatgggc gggcctcggt tgcccgcccc ggctcattcc gaggtttctt
     2461 gcctgtagca gcatgcctgc acgattcccg aatacacttt agcagacagg caatacactg
     2521 caaggcaggt gaccaccagt gtacagtcgc gcgctggcac tgatctttgc cctcagcctt
     2581 gtcatcggca tatcgggctg taacggcgaa tccgcctctg tccacggggt tgtcgagggt
     2641 ctagtcacag accaagtatc cggacgcccc gtaagagatg tctgggtgac gacaggatct
     2701 atagtagcct ggacagacgc tttgggcgcg tactggctat cactcccgca agggacacag
     2761 tcaatctgct ttcaacgtgc tggctatgcc cgaaagaccc tacccgtcac ggtggtcgga
     2821 agatcaaccg ttcagaccaa cttggagatg aacccgattc tgccagccgg accagtggaa
     2881 atatcgctta catggggcgc gtccccagta gatctcgatg ctcacctctg gacttccgga
     2941 ggctaccgca tcaaccatag ctgcggcgga tcactcacct ctccaccatg ggccaagctc
     3001 gactacgact atttcgccgg gcacgggaga gagacaataa cgattgccga ttttgctccc
     3061 gacacctatc actactccat acataactgt agccgcacac ccgccatcgc aggcagcggc
     3121 gctcaggtag acgtgtactc gcaatcgcag ctactataca ccttcatcgt tccgcacacc
     3181 ggacccgccg actacctcgt atgggacgtg ttcgagattg acggcgccac ccgagaaatg
     3241 gcgaggctgg acacgctccg ggagtgccca aactggacac tcgaacagat cggccctgtt
     3301 gacaagcccg aagccctaaa ctcggcaggc ttcaggccgg tggatgaatc gccgacagag
     3361 cagtagacca caccggctcg acagtgtcag ccaaatcctg gcgcaggagt ccccttgccg
     3421 tcccgccgtt ttcgctcgga gagggcatcc gccttctccc aacatgcacc cgggatcata
     3481 cccctctcga caaggcagcg cgaatcagcg tctgtatgcg agggagcatg cccgacacgg
     3541 aagccgcagt atatacaaga agcatcgcca tcgcgatgtt gaaaggcctt ccgtacctgc
     3601 ccagcaaccg ctgtatacct gtaccgaaca tggcccagca agatgtggag gcaagagaga
     3661 tggatcccaa aaaggctgag aagaagagca gagctaccgg agagtggtag tacggcgtta
     3721 tgaacgtagc agtaaccgta agtccgtaca ggatggcctt gggattgatg aactgcagta
     3781 ctacgcctgc gggaaaggag gtaaggtgtg cgtcgtcgtt tgtcgcatcc accttgctca
     3841 tgagcacgcg tgccgctaga tagagcatgt acgccccgcc gacggtctcg gcgataggct
     3901 tcatcttggg tactacggtg aataggaaca ggttcaggta gcttgagact atcatcacta
     3961 gaccgaatcc gacggctacg cccaggcgaa acggcaacgt gcgggcgaat ccatacttgc
     4021 tgcccatcac caaggacatg atgttattgg gtcctggtgt aaaggtgact atcagaacat
     4081 acgacagaaa cggaatgaca ttcggcattt ggcgcacctc aggcttccac aacgctttac
     4141 tgacctcgtg ccccgcggcg taaatgggac catgctgctg ggaccatact gcgtaggcat
     4201 gacatgtgta tttcaatgtg atgcgccgat tccctctttg cagtgcagga acgtttcatg
     4261 ctgtcacgaa tactgaccta tccaccagac tcatgtcaac acgcttagga ggccacccga
     4321 tgaacgcgtc tcgaatcgtc accctccaac gtgcacttcg cgaccaaggt ctggcatgcg
     4381 ccttcctcgt tccaggccct aatctcaagt acctcaccgg tcttcagctc gaggctttcg
     4441 aacgcgtgat ccttggcatc gtgccaccct acggcgatcc tgcgttcatc gtgccgcaac
     4501 tggaggcaga caaagtccgc ggtcatgtgg gcacccactt acattgcgac accgtctttc
     4561 catacacaga cgacgccgga cctcttgacg cactgcgtca ggcccttcgt acccttggcc
     4621 tcggccccga ccgtggcccc gcggcggtgg gggctgagtt tctccatatg cgcctactcg
     4681 agcgccaggt catcgaacaa gccagcgggc atatcgagtc ctgtcactcc atgcagtttc
     4741 gggatctcgg gccgataacg gcccgcatgc gctcggtgaa agacccagat gagctcgccg
     4801 ctctcaaacg ggcggctcgg atcgtggatg ccggcatcgc cgccgccgcg tccgcaattg
     4861 ccccaggcgt cacagagcgc gaagtcgcag cggcagctga acgcgctatg gctgcggccg
     4921 gcgcagacag catcccgttc aacgccgtgc tggccgggcc caactccgca ctcccgcatg
     4981 gccagaccag cgactacgcg atgcgcgacg gcgacatggt gatctgcgat atcggggcca
     5041 cataccgcgg gtacaatggc gacatcaccc gcaccttcgc cgtgggaaag cccacgtccc
     5101 agatggcccg gattcacagc attgtctacg atgctaatcg cgcagcctgc caagccgccc
     5161 gccccaaagt caccggggag cagcttgact ggatagcccg ggacgtaata acccgcgccg
     5221 gtttcggagg gtatttcacc caccgcaccg gacacgggct aggtttagag attcacgagg
     5281 agccgtatat cgtgcaaggc gccacagagc cactcaaagc gggcatggta ttcactatcg
     5341 aaccaggcat ctacatccct ggcgtcggcg gagtccgcat agaagatgat ctggcactca
     5401 ctgacgatgg ctgtatggta ctcactgcgt ctccgcgaga actggcctaa cctgattcag
     5461 tctacatatc ccgagaacac agcccactcc aacatcaacg acagctgggg tagggtgatc
     5521 tcggctttga tggccatctc aataagcacc ttgtcctgcc actccgtacg catgccggca
     5581 gttagagtcg tgccgggggt cgggtacact gattgcatga ctgccgtgac gcgcgctgct
     5641 gtcacctgga ggccaaatga cttgagctgc ttgtcactga catcatctac tgatacctta
     5701 tgccccttga tcatcttgac atcctggatg ggcaaccagt agcgctgacc gctggggcca
     5761 taaacccaca cagccttgcc cttatgctcc gcagcggcga atgtgacccc ttccggcagg
     5821 ttcatcacaa ccaacccgct ttcagtcctg agctgggtcg gccgcttggt caccgctgca
     5881 tctatctttc gctccttggg cggtttaggc gccttaacct tggcaggttt cttggcatcg
     5941 gctacagtcg aacccgcaga gcacaccatc gccatagcca gcagcgctat cagcatatgt
     6001 acaaatgccc gtctctttgt caaccagatt cactccctag ctgcaatgat tgaaccatct
     6061 ctactacaga cgggcgatga cccggcagtg ttccacaagt tgcgtgtacc agcgcatttg
     6121 cgttctactc ccccacgcgc cgacccaggt gccgcaagag gcgctcgggg tagttggtaa
     6181 tgatcccgtc taccccagcc tgtattaggt gtgtgaacac ccaatcgtca tcggcagtcc
     6241 atgcaaacac atcgatacca ctgctgttga gtaccttgac gagctcagcc gagactgctc
     6301 ccacattcat ccctgcgaaa ctcgcgccta cctcaagcac ctgctcgact atgcgtcggc
     6361 ctcggcctgc cgggtccaga acccacgtat cccggtagag tatgcccgcc ggagtctcgg
     6421 gcaacagccc gcgcatgatc cgcactgact cggcgtcgaa ggactgcagc attacgcggc
     6481 cttccatgcc ggcctcgtga atcacctgcg ccactctctc ctcgatgccc cgtgccttca
     6541 gttcgatgag aaacagtgcc ctctctttga aggcggcaag aacctcatcc agagtaggca
     6601 tgcgctctcc ggcaaacccg gagtccttcc atacgcccac atccaacgcc ttgagctgca
     6661 cgagtgtgag atttgcgacc gcaccctgac aattgccctt agctgttcga gacgtcgaag
     6721 cgtcgtgaat cacaatgagg tagccatcct tgctgccatg gacatccaat tcgatcatgt
     6781 cggcgccaaa cctgatagcc tgctcaaaag aggccatggt gttctcaggc gcaactcccg
     6841 acgccccccg gtgcgcgatc actagcgggc gggctgcccc cacgacatgc gtaatcgcct
     6901 taccagcgtg caccggcgcg gtcgcgcaga gcgcggccac catcagaagc cccgtaacca
     6961 gtctacacac acgaaacaca cacccgcgca tgcctactcc cccttgaccg caaaatcgcc
     7021 ctgtgcctta taggatcatt gggtacgtat gccacctgtt tccgctgtga ccagattgta
     7081 ctacgagcgt cagtcgacaa caatacaggg agtgctcagt tgtcgcagat gtgtgccaag
     7141 gacgagtact gctgggaaga cctgtccctg ggcgaggccg aagtcacgca agccctcgcc
     7201 ggcctgcgcg cctcgcaacc gggtagaccg atacggggca tcgcacagcc gggacatgct
     7261 cccgagccag ccgaagaaga agttgtcgac cccgacaac
//
LOCUS       DAOTAC010000109         7286 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_8685_length_7286_cov_4.215600, whole genome shotgun sequence.
ACCESSION   DAOTAC010000109 DAOTAC010000000
VERSION     DAOTAC010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7286
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8685_length_7286_cov_4.215600"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="PLM74_05935"
     CDS             <1..513
                     /locus_tag="PLM74_05935"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HON42659.1"
                     /translation="TDHIVAPGMLCEGKAAAIIDGSWALGAIQQAGIDYGVAPIPRLD
                     NGKYPAPFVSLKWWHVSSYSKRKTEAVKLIAALTTKDAMYKSFKNAEGIPPRLDVLDM
                     PDVKNKPEVAGYGAQANYGSILPDIPEMNVVWVPMNSAIEIALRGDRTVEDALADAVK
                     VIKQSIAELK"
     gene            762..1955
                     /locus_tag="PLM74_05940"
     CDS             762..1955
                     /locus_tag="PLM74_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HON42660.1"
                     /translation="MNTSRKVLLSLQHTIAMFGATVLVPYLTGLNPSTALLTAGLGTL
                     LFHLITGGTVPIFLGSSFAFIGVIQIVSKEFGMQYALGGIVAAGLVYVAVAVVVRFLG
                     YGFFQTLFPPVVTGSVIMVIGLTLSPVAIDMASSNWPIALAVFFIAAMSSLAFRGFLQ
                     MLPILIAVTAGYLISIAAGIVDFTAVSEAAWVGLPQLTFPAFRWEAIAVIAPVAVVTL
                     IEHWGDINANGAVVGRDFVKSPGLWRTILGDGLATTLAGLLGGPANTTYSENTATLSL
                     TGVYDPAILRGAAFFALGLAFFGKVGGVIRSIPTPVMGGISILLFGMIASVGISTLFS
                     GADMNSTRNRIIAAAVLVFGLSTVVVPLGPFKFAGMSLATVIGSLLNQVLPKDKPESK
                     AAEGK"
     gene            complement(2254..3039)
                     /locus_tag="PLM74_05945"
     CDS             complement(2254..3039)
                     /locus_tag="PLM74_05945"
                     /inference="COORDINATES: protein motif:HMM:NF005413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HON42661.1"
                     /translation="MDSVSARENVTALWAEYYSTRNREVREQLILNYLDLVKYIVGRM
                     PQATIPGVDYDDLLGYGVMGLMDAVERFDLSRGVRFETYAMTRIRGAIIDHLRSMDWV
                     PRSVRQKARQVQQAIGRAEARLDRSATDQDIAEELGIDIETFDRWTWEISKATYLSLD
                     ELITVDEAHNTATLIDFISDDTSPDPELVMEVDELKSALADAIDALPERERMVISMYY
                     YDELTIKEIASVLGVSESRVSQLHTRAMMRMRADVKRAAGQCV"
     gene            complement(3039..3728)
                     /locus_tag="PLM74_05950"
     CDS             complement(3039..3728)
                     /locus_tag="PLM74_05950"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HON42662.1"
                     /translation="MQLPLFEPGGQIRVRLAAKDDFPSFATKIEGKADSGYWIYAPYT
                     DDYRSMVRAQDELEVSMMWKGAVYFFGATVIGEKHDRVKLLLISDPEFIRREQRREFV
                     RVPVMFDAQIACNPATQDASADEWVTVVASDISGGGIGLFIWNKDVPAWLLARRRIRV
                     AMPIGQDGSTVKVMGQVVRLEKPDEGPTDGVNVGVKFIEISESERRVIIRFVFDRQRE
                     LIRKSAHRSDD"
     gene            complement(3739..4656)
                     /locus_tag="PLM74_05955"
     CDS             complement(3739..4656)
                     /locus_tag="PLM74_05955"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HON42663.1"
                     /translation="MSDQAAELRGLVRQFRGQDDTVCRQTTRSGQPDAVRIVAVTSGK
                     GGVGKTNFVVNLAMALADAGTRVTILDADLGLANVDVLLGMTVRHNLHHVINGTLSIA
                     DIVLPGPRGIEVIPGASGLRDIADMKETARHKLIESLSDAVRDRDLLLVDTGAGVSRN
                     VLDFVLAAQEVILLTMPEPTAMTDAYAMIKLISRSNPDAAVRVVVNCAPSRQEAENVV
                     EQLNLLSRRFLSFSVDPLGYVPYDQSVVKAVRKRQPFVIAYPHSPASAAISSIAGDLR
                     GLPTPRGYEPGVALFIRRLLRAPASDYLL"
     gene            complement(4700..5980)
                     /gene="flhF"
                     /locus_tag="PLM74_05960"
     CDS             complement(4700..5980)
                     /gene="flhF"
                     /locus_tag="PLM74_05960"
                     /inference="COORDINATES: protein motif:HMM:TIGR03499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HON42664.1"
                     /translation="MRVKRYEGSTMKDALDQVRQELGPDAVILEARRSRGRGLGALLS
                     ILGFGKAPVFEVMAAADGDDGGGRDGDGASGFTAVTWVPSPLTARAKAETADEAQAEA
                     TEPALQASAQTMTGPTMQVVTPPRMPEVPLAPAISPSGPLATLKVLEREAAVRARAAG
                     LSPEMADLYAHLVDEDVAEDVAFAVVHQLGMADAPQLETEQFREWASEHAAGLVRTSG
                     PIQIVRDRRKVVAFIGPTGVGKTTTIAKIAAGFALFAKARVALVTADTYRIAAIEQIR
                     SYAEIIGIPCEVVHSPKDMEEVLAGLADYELVLIDTAGRSPNNPARMYELRNMIDAAM
                     PEEIHLVLSSTTRRRDLDNLVDRFAAVGFDRVILTKMDESMTFGAIFNVHAKCAKPFS
                     YFTNGQSVPEDIDTATQSQVARMVLAKWRIAGGL"
     gene            complement(5970..>7286)
                     /locus_tag="PLM74_05965"
     CDS             complement(5970..>7286)
                     /locus_tag="PLM74_05965"
                     /inference="COORDINATES: protein motif:HMM:NF012973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HON42665.1"
                     /translation="TATGIVVTRAASESDLGRDVTSQMLAQPRALAIAAGLLLLLGMV
                     PGLPKAPFLILAAAAGFLAYRVTRSAREAEQAAAVPHLPAPGVAEPEDVMALLRVEPM
                     ELNFGYALIPLVDADQGGDLKERVAAVRRQIAMELGIVVPSVRIRDDIQARSNEYIVR
                     IRGVEVDRGEVIPDAYMAMNPGTATEELDGIQTREPAFGLPAVWVSASRRAEAEMMGY
                     TVVDPSSVIATHLSEIIKANAAELLSRQDTRNLIDNVKKTAPAVVEDLIPAQMSVGEV
                     QGVLQNLVRERISIRDLITILEALGDAAGTYKDVDALTEYVRSRMGRSICSQYAGPDG
                     MAVTTLEPSLEQTLMDSLDITEHGTFLAPEPAMYRALIASLAAESEHMAAAGQQPIVL
                     TSTSLRPHLRRLVERAIPQLVVLSYSEIVPEVKVNAVGMVSLGNAS"
BASE COUNT         1437 a         2401 c         1950 g         1498 t
ORIGIN      
        1 acagaccata ttgtcgcccc cggcatgctc tgtgaaggca aagccgccgc catcatcgac
       61 ggatcctggg ctctcggtgc catacagcag gcgggcatcg attacggcgt cgcccccatt
      121 cccagactcg acaacggcaa gtatccggcc ccattcgtca gtctcaaatg gtggcatgtc
      181 agttcctaca gcaagcgcaa gacggaggcc gtcaagctca tcgccgctct gaccaccaag
      241 gatgcaatgt acaagtcctt caagaacgct gaaggcatac ccccgcgcct tgatgtgctc
      301 gacatgcccg acgtcaagaa caaacccgaa gtcgccggct acggcgccca ggccaactac
      361 ggatcgattc tcccggacat accggagatg aacgtcgtgt gggtccccat gaatagcgcc
      421 atcgagatcg ccctacgcgg cgaccgcaca gtcgaggatg cgctggccga tgccgtgaag
      481 gtgatcaagc agagcattgc agagttgaag taactgaagg gcgctcgtac agatacgccg
      541 gcccgctacg aatggggcgg gccggcgtca cagggcaatg ccgcagggca acgcacaagg
      601 caatacacag agcaatgcca tcagcccagg ttgttttcga attactctcg aattgtaatg
      661 ttcttgttat tgcagcaggc gccgggctgt gctagattga gagcagtaca tttcaccaaa
      721 taaacgagtc ccgtgagact cgatttgagg aggcgtatca gttgaacacc agcagaaaag
      781 tgctgctttc gctgcagcac accatcgcaa tgttcggcgc aacagtcctg gttccctacc
      841 tcaccggcct caaccccagc accgccctgc tcactgcagg acttggaact ctcctcttcc
      901 acttaataac cggcggcaca gtccctatct tcctcggatc atctttcgcg ttcatcggag
      961 tgatccagat cgtatccaaa gagttcggaa tgcaatacgc actgggcggc atcgtagccg
     1021 caggtctggt ctacgtggca gtagctgttg ttgtcagatt tctcgggtac ggcttcttcc
     1081 agaccctttt cccgcccgtg gtcacaggca gtgtgatcat ggttatcggc ctgaccctat
     1141 cacctgtcgc catagacatg gccagcagca attggcccat cgccctggcg gtcttcttca
     1201 ttgccgccat gtcgtcgctg gcgttccgcg gcttcctgca gatgttgccc atactgatcg
     1261 ctgtaactgc tggttacctg atatcgatcg ccgcaggtat cgtcgacttc acggccgtat
     1321 ctgaagcggc ctgggtcggc cttccgcagc tcacctttcc cgcctttcgc tgggaggcaa
     1381 ttgccgtcat cgcgcccgta gcggtcgtaa ccctcatcga gcactggggc gacatcaacg
     1441 ccaacggcgc cgtcgtaggt cgcgacttcg tcaaaagccc cggcctgtgg cgcaccatcc
     1501 tcggagacgg cctggccacg acgctcgccg gcctgcttgg cggacccgcc aataccacct
     1561 acagcgaaaa cacagccacc ctcagcttga ccggcgtata cgacccggcc atcctgcgcg
     1621 gggctgcatt cttcgctctt ggtttggcct tcttcggcaa agtaggcggc gtgatcagga
     1681 gcattcccac gcctgttatg ggcggcatca gcatcttgct gttcggcatg atcgcctcgg
     1741 tgggcatttc cacgctgttc tccggcgccg acatgaactc cacccgcaac cgaatcatcg
     1801 cagcggcagt tctggtgttc ggcttgtcca cagtagtggt cccgcttggg cccttcaagt
     1861 tcgccggcat gagcctggca acggtgattg gatctctgct caatcaggtc ttgcccaagg
     1921 acaagcccga gagcaaggcc gctgagggca agtaggtttc gcagagtcgt gatagactga
     1981 gagcccatgt aggcaggcat gcaggatgga catgaacgat ggacacaata catcgcaaca
     2041 cggggccggc gtgcagcgga gcatgccagg ccccgtgtcc ttgcagtact agtaagcatc
     2101 gcgaagtgga aatccgagca ggcgttgcca ggcagagtga cggcgagaag gacatcgtcg
     2161 cgaaacgacc tttgagctgg ttatgctgcg gcgggcctgt cgccaacaaa cggccgcaag
     2221 gggaaggatc tggggcccta aaagcaacga tcgctacacg cactgcccgg cggcgcgttt
     2281 cacgtccgcc cgcatccgca tcatggccct ggtgtgtagt tgcgacactc tggattccga
     2341 cacaccaagt acactggcta tctccttgat agtcagttcg tcatagtagt acattgagat
     2401 gaccatacgc tcacgttcag gcaacgcgtc tatggcgtcg gcaagcgccg acttgagttc
     2461 gtcgacttcc atcacgagtt cggggtcagg actggtgtcg tcagatatga agtcaataag
     2521 cgttgcggta ttgtgcgcct cgtccactgt gatcagttcg tccagcgaaa gataagtcgc
     2581 tttgctgatc tcccaggtcc acctgtcgaa agtctcgatg tcgatgccga gttcctcagc
     2641 tatgtcttgg tcggtcgccg atctatccag gcgagcttca gcccttccaa tggcctgctg
     2701 gacctgccgc gctttctgcc tgacggaacg cggtacccag tccatgctcc tcaggtgatc
     2761 aatgatggcg cccctaatcc gcgtcatcgc gtatgtctcg aacctcacgc cccgcgacaa
     2821 gtcaaaccgc tccaccgcgt ccatcaaccc catgactccg tagcccagca ggtcatcgta
     2881 gtcgacaccg gggattgtag cctgaggcat gcgcccgacg atgtatttca caagatccag
     2941 gtagttcagg attagctgct ccctgacctc cctgttcctc gtggagtagt attcggccca
     3001 cagagcagtc acgttctcac gtgcactaac gctatccatc aatcatcact ccggtgagct
     3061 gatttcctta tcagctctct ttgcctgtca aagacaaaac ggattatgac tctgcgctcg
     3121 ctctcggaga tctctatgaa cttaacgccc acattgacgc cgtcggtcgg gccctcatcc
     3181 ggcttttcca gccgcacgac ttgccccatg accttgactg tgcttccatc ctggcctatg
     3241 ggcatggcaa cccggattcg ccgccgcgcc aaaagccacg caggcacgtc cttgttccat
     3301 atgaacaggc ctatgcctcc gccgctaatg tcgctcgcca ccacggtcac ccattcatcg
     3361 gccgacgcgt cctgtgtcgc cgggttacac gcgatttggg cgtcaaacat taccggaacc
     3421 ctgacgaatt ctctcctctg ttcgcggcgg atgaactccg gatccgagat cagcagcagt
     3481 ttcacccggt catgcttctc gccgatcaca gtcgcgccga agaagtagac ggcgcccttc
     3541 cacatcattg acacttccag ctcgtcctga gctctgacca tcgaccgata gtcatcagtg
     3601 tagggcgcgt agatccagta acccgaatcc gcctttcctt cgatcttcgt cgcgaaactt
     3661 ggaaagtcgt ctttggctgc caacctcacc cggatttgac cgcccggctc aaatagcgga
     3721 agctgcatcg ctcaatcctc acagcaagta gtccgacgcc ggcgccctga gtaaccgccg
     3781 gatgaacaat gccacgcctg gctcgtatcc cctgggcgtc ggcaaacccc tcaagtcacc
     3841 ggcaatggaa gagatagcag cagaggccgg tgaatgaggg tatgcaatca cgaagggctg
     3901 cctcttgcgc actgccttca ctaccgactg atcgtagggc acgtaaccga gcggatcaac
     3961 actgaaactc aggaacctcc gcgacaacag attcagctgt tccactacgt tctcagcctc
     4021 ttgtcgacta ggcgcgcagt tcaccacaac ccgcacggca gcatccgggt ttgagcgaga
     4081 gatcagtttg atcatggcat aagcgtcggt catcgcagtc ggctcaggca tggtcaggag
     4141 aatcacctcc tgtgccgcca gcacaaaatc gagcacgttc cgcgacacac cggctcccgt
     4201 atctacaagc agcaggtcgc gatcgcgaac ggcatccgac aatgactcga tcagcttgtg
     4261 gcgtgcggtc tccttcatat cggcgatatc gcggaggcca ctggcgcccg gtatgacctc
     4321 gataccgcga ggcccgggaa gcacaatgtc cgcaatcgac agcgttccgt ttatcacgtg
     4381 atgcaggttg tgcctcacgg tcatccccag cagcacgtca acattggcca agcccaggtc
     4441 ggcatcgagt atcgtaacgc gcgtgcccgc gtcggccagt gccatagcca gattcaccac
     4501 gaaattggtc tttccgaccc cgcccttgcc cgaagtcaca gccacaatcc gtacagcgtc
     4561 aggctggccg ctccgcgtag tctgccggca gacagtatca tcctgccccc tgaactggcg
     4621 gacaagccct cttagctcag cagcctgatc agacatgtgc tactccctcc tgtacccctc
     4681 cttaggtcgg cgacttccac tacagccctc cggctatccg ccattttgcc aggaccatcc
     4741 tggccacttg ggactgagta gcggtgtcga tgtcctcggg cacactttgc ccattggtga
     4801 aatacgagaa cggtttggcg cacttggcgt ggacattgaa aatggcgcca aacgtcatgc
     4861 tctcatccat cttggtgagt atgacacggt caaacccgac cgccgcgaac ctgtccacaa
     4921 ggttatcgag atcgcgccgc cgagtagtgg acgagagcac cagatggatc tcttcaggca
     4981 ttgctgcgtc gatcatgttc ctgagctcgt acatcctggc cggattattg gggctccggc
     5041 ccgccgtgtc aatcaacact agttcgtagt cggccaaacc tgccaggacc tcttccatat
     5101 ctttagggct atggaccacc tcgcatggaa tgccgattat ctcagcgtag ctccgtatct
     5161 gctctattgc cgcaattcgg taagtgtcgg ccgtcaccag cgctacacgg gccttcgcaa
     5221 acagagcaaa tcccgccgct atcttggcta tcgtggtggt tttgccgact cccgtcggcc
     5281 cgatgaaggc cacgaccttc ctgcggtcgc gcacgatctg tataggcccc gacgtccgta
     5341 ccagcccggc ggcgtgctcg cttgcccatt ccctgaactg ctccgtttcg agctgcggcg
     5401 catcagccat acccagctgg tgcacaacgg cgaatgccac atcctcagct acgtcctcat
     5461 ccactaggtg cgcataaagg tctgccatct caggggataa gcctgcagcc ctggccctta
     5521 ccgccgcctc ccgctccagc accttgagcg tggccagcgg cccggacgga ctgatcgcag
     5581 gcgccagcgg cacttcaggc atcctgggcg gcgtcactac ctgcatcgtc ggccccgtca
     5641 tcgtctgcgc cgacgcctgc aatgccggtt cggtcgcctc ggcctgcgcc tcgtctgcgg
     5701 tctctgcctt cgcccgcgcg gtcaaaggcg acgggaccca tgtaacagcc gtaaagccgc
     5761 tggcgccatc gccatcacga ccgccaccgt catccccatc tgcggccgcc atcacctcga
     5821 acaccggggc cttcccaaag cccaggatgc tgagcaatgc cccgagcccg cggcctcgcg
     5881 accgcctagc ctccagaatc actgcgtcgg ggcccagctc ctgcctgacc tgatccaggg
     5941 catccttcat agtcgaacct tcgtagcgct taactcgcat tgccaaggct caccatccca
     6001 accgcgttca ctttgacctc cggcacaatc tcgctgtacg acaacaccac cagctgcgga
     6061 atggccctct ccaccagacg tctgaggtgg ggcctgaggc tcgtagatgt gaggactatc
     6121 ggctgctgcc cggctgccgc catgtgttcg ctctcagccg ctaggcttgc gattagggct
     6181 cgatacatag ccggctcggg ggcaaggaat gtgccgtgct cagtgatgtc aagcgaatcc
     6241 atgagagtct gttccaagga aggttccaac gtagtcacag ccatcccgtc cggccctgcg
     6301 tactggctgc atatggagcg ccccatccgc gaccgcacgt actctgtaag ggcatcgacg
     6361 tccttgtacg tgccggccgc atcgcccaga gcctccagga tcgtgattag gtcgcgaatg
     6421 gatatgcgct ccctcaccag gttctgcagc accccctgga cctctcccac gctcatctgg
     6481 gccgggatca ggtcctccac tactgccggc gcagtcttct tgacgttgtc gatgaggttg
     6541 cgcgtgtcct gccggctcag gagctctgct gcgttggctt tgattatctc cgataggtgg
     6601 gtggctatga ccgaagaggg gtcaaccacg gtgtatccca tcatctccgc ctctgcccgc
     6661 ctagacgcac tgacccacac cgcaggcagt ccgaatgcag gttcgcgcgt ctgtatgccg
     6721 tctagctcct cagtggctgt gccggggttc atggccatat acgcgtcggg gatgacctcg
     6781 cccctatcca cttccacgcc cctgattctc acgatgtact cattcgaccg cgcctggata
     6841 tcatcgcgta tccgcactga tggcacgact attcccagct ccatcgcaat ctggcgtcgc
     6901 acggccgcca ctctctcctt cagatcgccg ccttggtccg catcgaccag cggtatcaga
     6961 gcgtacccga aatttagctc catgggctcc acccggagca gagccatcac atcctcgggc
     7021 tcggcaaccc cgggcgccgg aagatggggc actgcggccg cctgctccgc ctcccgcgcc
     7081 gacctcgtga ctctgtaggc cagaaagcca gcagcggccg ccaggatcaa aaacggcgcc
     7141 ttgggcagcc ctggcaccat gcccaaaagc agcagaagcc ctgccgcaat cgccagcgca
     7201 cgcggctgtg ccagcatctg tgatgtgaca tcgcggccaa ggtcggattc ggacgcagcc
     7261 cgcgtcacca cgatgcctgt ggcggt
//
LOCUS       DAOTAC010000110         7157 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_8877_length_7157_cov_5.311321, whole genome shotgun sequence.
ACCESSION   DAOTAC010000110 DAOTAC010000000
VERSION     DAOTAC010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7157
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8877_length_7157_cov_5.311321"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..174
                     /locus_tag="PLM74_05970"
     CDS             <1..174
                     /locus_tag="PLM74_05970"
                     /inference="COORDINATES: protein motif:HMM:NF024045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HON42666.1"
                     /translation="RAAAALDEVARAAGDGVPADMLSIDLRRAIDALGEITGETARED
                     IVDRIFEQFCIGK"
     gene            206..1942
                     /gene="mnmG"
                     /locus_tag="PLM74_05975"
     CDS             206..1942
                     /gene="mnmG"
                     /locus_tag="PLM74_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004093313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HON42667.1"
                     /translation="MDYDVVVVGLGHAGCEAALACARMGCLTLGITVSMDNIAQMSCN
                     PSIGGPAKAQLVREIDALGGEMALAADDTFMQVRMLNTSKGPAVRAIRAQIDKADYQA
                     RMRMALESQPNLVLRQAMVSGLVIRGGAVAGVVTDMGEISARAVVLATGTYLGGRVHM
                     GELSYASGPDRMPPSVELGENLRRLGFSVHRFKTGTPARVDGRSVDYSKMVPQPGEDL
                     PYGFSFVRKPAPREQLCCWATWTNERTHEIIRDNLHRAPMYSGAITGTGPRYCPAIET
                     KIVRFPERPRHQVYVEPQGRRTQEMYVAGVSTSLPMEVQEEMLRSIPGLENAHIVRYG
                     YAIEYDCIDPTQLFPTLEAIAVRGLYCAGQINGTSGYEEAAAQGLVAGINAAICVTRS
                     DVPRVVLDRSEAYIGVLIDDLVSKGADEPYRLMTARAEYRLLLRQDNADLRLTPVGRS
                     AGLVDDERWSRFKERREAIEMVRAGHALPHSYRNYADDAKEQVEIETRFAGYIAKQEK
                     QVQEFRKLERWTIPGDIDYGSLSRLSREARERLAQVRPMSVGQATRVSGVSPADVMAL
                     TVHLREMAREGA"
     gene            1959..2735
                     /gene="rsmG"
                     /locus_tag="PLM74_05980"
     CDS             1959..2735
                     /gene="rsmG"
                     /locus_tag="PLM74_05980"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HON42668.1"
                     /translation="MLDETPITEAPFRETLEQAARELDVQIDQGQLDMFCAYKQLLIE
                     ANRSTNLTSITEDAEIAIKHFADSLTCLRVVNLRNVTSIVDVGTGAGFPGLVLKIMNP
                     AARVLLIDSIGKKVDFVREVSASLGLNGVESVQARAEELGRHSRYRERFDLAVARGVS
                     GLSPLAEYCLPFVRLGGLFVAMKGPKVAQEMGSGARAVAILGGGELSTCQLELPFEAG
                     VRVLVSATKRRGTPDQYPRRPGIPERRPLGQAATNRKSPG"
     gene            2821..3588
                     /locus_tag="PLM74_05985"
     CDS             2821..3588
                     /locus_tag="PLM74_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011879606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HON42669.1"
                     /translation="MGRVIAIVNQKGGTAKTTTAVNLSAYLAMAGRRVLLVDMDPQGN
                     ATSGLGIAKREVETCIYRVLIEEMPIAEAIVHSTVQGLDVVPATLNLAGAEIELVSVI
                     SREHRLRSALQPLRAAYDYMLVDCPPSLGLLTINALTAADSVLVPIQCEYYALEGVSQ
                     LMRTVDLVRRHLNPGLEIEGIVLTMYDSRTNLSQQVADDVKANLGDYVFRSVIPRNVR
                     LSEAPSYGLPISLYDDRSKGAEAYRGLAAEVMEREGA"
     gene            3591..4460
                     /locus_tag="PLM74_05990"
     CDS             3591..4460
                     /locus_tag="PLM74_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HON42670.1"
                     /translation="MAAKPRGLGKGLNAIVGADIVSEVIDRPRAIEIPVDRLSPNPFQ
                     PRRSFTEEGLEQLAESIRQHGVLQPIVVRPSGDGYQLIAGERRWRAAQIAGLRRIPAV
                     VRELDDPGMVQLALIENLQREDLNPIEEASAYRRLMDEFGMTQERLSSAVGRSRPAIA
                     NAVRLLNLPVEIQRAVEERRLSEGHARCLLAITDQGDQLKVAAEIIANGLNVRQAEEL
                     VKGIARNVSRETTRKRSSGEDPDAVHLTQRLGQRLGTKVKLSGSVSKGRIEIEYYSAE
                     DLDRILEIILGNG"
     gene            4553..5065
                     /locus_tag="PLM74_05995"
     CDS             4553..5065
                     /locus_tag="PLM74_05995"
                     /inference="COORDINATES: protein motif:HMM:NF025935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HON42671.1"
                     /translation="MVGLANLVETQKDIVVLVLSLILLVSIAVFIMASARTRRLEKRY
                     RVLLGNADGRSAEALLLDYIARTDSLAAQLEQTQSELTDLCSLCQGHVQKVGILRYNA
                     FDDVGGEQSFSVALLDANATGIVISGLCGREETRVYAKPVKAGRSTHNLSDEEITAIA
                     RAIEGYSGSR"
     gene            5406..6374
                     /locus_tag="PLM74_06000"
     CDS             5406..6374
                     /locus_tag="PLM74_06000"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HON42672.1"
                     /translation="MTIPSLALHVGLPIAIGLAVLLVGSYVHMTHGMVQLSRDRQSKG
                     VAIEELLRENELLKEEKDERIAQLKELTEKADKLAAAVDKVVGENSKIWDIVKGPGAA
                     KSTSSSSSSAVRGATSRGGDPGADRGEPVIGSSSSAWLGPEEEIRSSPAQDEDELAVA
                     ASEALARLSELTNSTQKDMYSLRSSATQYRNKLNHTPSIAPTKGKITSGYGNRLHPIL
                     RVRRLHEGVDIAAPRGTPIVATADGVVARAGWLGSYGNVVEISHGYGITTFYAHNSRN
                     AVKVGATVKRGQVIAYVGSTGLSTGAHVHYEVRVNKRPVDPSSYMK"
     gene            6415..6834
                     /locus_tag="PLM74_06005"
     CDS             6415..6834
                     /locus_tag="PLM74_06005"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HON42673.1"
                     /translation="MFRRDRQLSSGDTKVDTYIGKGARLQGTVSVSGTVRVDGFVDGV
                     VEAEKDVIIGQGGLVTASITAANVTVAGELRGNVKASGRLEIARTGKVLGDVAIASFV
                     VEEGAAFLGKCEMLKTDARHPNAHPDTMENEPPGTDE"
     gene            6931..>7157
                     /locus_tag="PLM74_06010"
     CDS             6931..>7157
                     /locus_tag="PLM74_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018546116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HON42674.1"
                     /translation="MRFRNRIEAGRILGVEMAKGTWSDPLVLALPRGGVPVAEHVALA
                     IGAQLDVIVSRKIGSPFNPEMGIGAVGPDGNV"
BASE COUNT         1599 a         1869 c         2299 g         1390 t
ORIGIN      
        1 agggcggcgg cggcacttga tgaggtggcg agagcggccg gcgacggcgt gccggctgat
       61 atgctatcca tcgacttacg gcgtgccatc gacgcgttgg gcgagataac gggcgagacg
      121 gcccgcgagg acatagtgga caggatattc gagcagttct gcatcggcaa atgactccat
      181 cgtgagagat gagaggtgag gcgcagtgga ttacgatgtg gttgttgtcg gtctggggca
      241 tgctgggtgt gaggctgcgc tcgcctgcgc ccggatgggc tgcttaactc tggggattac
      301 ggtgagcatg gacaacatcg ctcagatgtc gtgcaatccg tcaataggcg ggcctgcaaa
      361 ggcccagtta gttagagaga tagacgctct tgggggcgag atggccctgg cggcggacga
      421 cactttcatg caggtacgga tgctcaacac ctccaagggc cctgcggtga gggctatccg
      481 ggcgcagatc gataaggctg attaccaggc gcggatgagg atggccttgg agagccaacc
      541 gaacttggta ctcaggcagg cgatggtgtc aggtctggtg atccgcggcg gagctgttgc
      601 cggcgtagtg accgacatgg gcgagatatc ggctcgcgca gtggtgcttg caactgggac
      661 ttatctgggc gggcgtgtgc acatgggcga actaagctac gcatcgggcc cggatcgcat
      721 gcctccttcc gttgaactgg gagagaacct tcggaggctg ggtttctccg ttcatcggtt
      781 caaaacgggc actccggcgc gcgtcgacgg gcgaagtgtg gactactcga agatggtccc
      841 ccagccaggc gaggacctgc catatggatt ttcatttgtg cgcaaacccg ctcccaggga
      901 gcagctttgc tgctgggcga cgtggactaa cgagcgaaca cacgagataa tacgcgacaa
      961 cttgcaccgt gcccccatgt acagcggtgc tataaccgga accgggccgc gctactgccc
     1021 ggcgatcgag acaaagatcg ttcgttttcc ggaacgtccg cgtcatcaag tatacgtcga
     1081 accgcaggga cgacgaaccc aagagatgta tgtggccgga gtgtccacta gcctgcccat
     1141 ggaagtccag gaagagatgc tcagaagtat ccccggcctg gagaatgcgc acatcgtcag
     1201 atatggttat gcgatcgagt acgattgcat tgatccaact caactctttc cgacccttga
     1261 ggccatcgct gtgcgcggac tgtactgtgc cgggcagatc aatgggactt ctggatacga
     1321 agaagcagca gctcagggct tagtggccgg catcaacgcc gcgatttgcg ttacgcgatc
     1381 cgacgttcct cgcgtagttc tcgacaggtc tgaggcatac atcggcgtgc ttatagacga
     1441 tctagtctcc aagggagcgg atgagcccta ccgcttgatg actgccagag ccgagtaccg
     1501 gctccttctc aggcaggata acgcggatct caggctgacg cctgtgggcc gaagcgcagg
     1561 gctcgttgat gatgaaaggt ggagcaggtt caaggagcga cgtgaggcca ttgagatggt
     1621 gcgggcgggc catgctcttc cacattcata tcggaactat gccgacgacg cgaaggagca
     1681 ggtggagata gagacccggt tcgccggcta tatcgccaaa caggagaagc aggtgcagga
     1741 gtttaggaaa ctcgagagat ggacgattcc cggcgacatt gattacggtt ccttgtccag
     1801 gctatctcga gaagccaggg agcgcctggc tcaggtaagg cccatgtcgg ttggccaggc
     1861 cacacgcgta tcgggcgtgt cgccggccga tgtcatggcc ctgactgtgc atctgagaga
     1921 gatggcccgg gaaggtgctt gagcggaggg caacagccgt gcttgacgaa acccccatta
     1981 cggaggcgcc tttcagagag accctggaac aggcggccag ggagctagac gtgcagatcg
     2041 accagggcca gctggacatg ttctgcgcct acaagcaact cctgatcgag gcgaacagga
     2101 gcaccaacct gacttccatc accgaggacg ccgagatagc catcaagcat ttcgcggact
     2161 ctctcacgtg cctacgcgtt gttaatctgc gcaatgtcac gagcatcgtg gatgtcggaa
     2221 ccggagccgg tttcccggga ctggtgctga agatcatgaa ccctgcagcc agagtgcttc
     2281 tcatcgacag tatcggcaag aaagtcgact ttgtccgaga agtctcggca tcgctcggcc
     2341 tgaatggcgt ggaatcggta caggcacggg ccgaggaact gggcaggcac tcgcgttata
     2401 gagagcgatt cgaccttgca gtggcgcgtg gagtgagcgg cctatctcct ctcgccgaat
     2461 actgccttcc gttcgtgaga ttgggcggat tgttcgtggc gatgaaggga cccaaggtgg
     2521 ctcaggagat ggggtccggt gcccgcgctg tggctatcct gggaggcgga gagctcagca
     2581 cgtgccagct agaactgccg tttgaggcag gtgtgcgggt actggtgagc gcgaccaagc
     2641 ggaggggcac acctgatcag tatccgagga gaccgggaat acccgagcgt cgccccctgg
     2701 gtcaggcagc gacaaacaga aaatcacccg ggtgagacgt gcatttctga cccctcttgg
     2761 tgaaggacat tgccgaacag cggagaagtc ttgcacagga tgctaggggg gaacgccgca
     2821 ttgggcaggg ttatcgctat cgtcaatcag aagggcggaa cggccaagac taccacagcc
     2881 gtgaacctca gtgcctacct ggccatggcc ggccggcggg tcttgctcgt cgacatggac
     2941 cctcagggca acgccacatc aggcctcggt attgccaaac gtgaagtaga gacctgcatc
     3001 tatcgcgtgc tgatcgaaga aatgcccatt gcggaagcga ttgtgcactc caccgtgcag
     3061 ggtctggatg tcgttccagc caccctcaat ctggcgggcg ctgaaatcga gctggtcagc
     3121 gtcatctccc gggagcatcg actccgttcc gcactgcaac ctctgcgcgc agcctatgac
     3181 tacatgctgg tggactgccc tccctcactg ggactcctca ccataaacgc ccttacggcc
     3241 gccgattccg tgctcgttcc catacagtgc gagtactatg cgctggaggg agtaagccag
     3301 ctgatgcgta ctgttgatct agttcggcgt cacctgaacc cgggcctcga aatcgaggga
     3361 attgtgctga cgatgtacga ttccagaaca aacctgtcgc agcaggtagc agacgatgtg
     3421 aaggcaaacc tcggagacta cgtgtttcgg tccgtcattc ccaggaacgt gaggctcagc
     3481 gaagccccga gctatggcct tccgatttcg ctatatgatg acagatccaa gggagctgag
     3541 gcctacaggg gccttgcggc agaagtaatg gagcgggagg gggcctgaag atggccgcga
     3601 agccaagggg actaggcaaa gggctcaatg ccatcgtagg ggcggatatt gtgagcgagg
     3661 tcattgatag gcctcgagct attgagatac ctgtcgaccg tctttcgcct aacccatttc
     3721 agccacgccg atccttcacc gaagagggcc ttgagcagct cgccgagtcc atacgtcaac
     3781 acggcgttct ccaacccata gtcgtcaggc cctcagggga tggataccag ctgatagccg
     3841 gcgagcgccg atggcgagct gcccagatcg ccgggctgcg acgaatccca gcagtagtca
     3901 gggagcttga cgaccctggt atggtgcagt tagctctcat tgagaacctc cagagggagg
     3961 acttgaaccc cattgaggag gcttcggctt accggagact catggacgag ttcggtatga
     4021 cccaggagcg actgtcaagc gccgtaggta ggagcagacc cgccattgca aacgcggtgc
     4081 ggctgctcaa cctgccggta gagatccaga gagctgttga ggagagaaga ctgagcgaag
     4141 ggcatgcccg gtgccttcta gctattaccg atcagggcga tcagctgaag gttgctgcgg
     4201 agatcattgc caatggactc aacgtaaggc aggctgagga actagtcaag gggatcgcac
     4261 ggaatgtttc acgtgaaaca actcgaaagc gaagttctgg agaggatccg gacgcggtac
     4321 atctgaccca gcgactgggt cagagacttg gcacaaaggt gaagttgtcc gggtctgtca
     4381 gcaaaggcag gatcgagatc gaatactact ctgcagagga tctggatcga attctcgaga
     4441 tcatcctggg caacggatag cggtcgatca cagatcacac caggggtcat gagatacgtt
     4501 gggcatagga gggggggacc gaggccccac agggccatga ataggtatgg aaatggtcgg
     4561 attagctaat ctggttgaaa cacaaaagga tatagtggtt cttgtcttgt ccttgattct
     4621 attggtcagc atagccgtgt tcatcatggc ttcggcaaga accaggcgtt tggaaaagcg
     4681 ctatcgcgtt ctcctcggca acgccgatgg aaggagcgca gaggcgctgc tactcgacta
     4741 catagcgcgg acggactccc ttgcggccca gctagaacag acacagagcg agctgaccga
     4801 cctctgcagc ttgtgccagg ggcacgtgca gaaggttggg attctgcgct acaacgcctt
     4861 tgacgatgtg gggggcgagc agagtttctc cgtggctctt ctggacgcca acgcgaccgg
     4921 aatcgtgatc agcggattat gcggccgcga agagacgagg gtctatgcga aaccagtgaa
     4981 ggcagggcgc tcgacacata atctatctga cgaggagatc accgccatag cacgggcaat
     5041 cgaaggatac tcgggatcac gctgatcaaa atcgagaaca tcagcaggaa ttcgcgtaaa
     5101 tgagtagaat gtgagacgat ggctaagtat gccggcaatt gcggtcatgg aatgcaaagc
     5161 ctggcaagta ctggtcatac tattaatacg gggcagttgg cacttgtgcc acggatgccg
     5221 gggggcgcat acacgaattg cctggaaagg gggaagtgcc gcattctttc ttatgcccga
     5281 gtaaaggggc ttccagcggc acgtttccgg tgatactgcg gttgcttccg cggcgctttc
     5341 gccgcaaacg acgtggatca cttacattga tggccatccc gacttcggga ggacccccga
     5401 agtcggtgac cattccatct ctggctcttc acgtgggtct accaatcgca ataggactgg
     5461 ctgttctctt ggtcgggtct tacgtacata tgacgcatgg catggttcag ctatcccggg
     5521 acaggcagtc caaaggcgtt gcgattgaag aactcctcag ggagaatgag cttcttaagg
     5581 aagaaaagga tgagcgcatc gcccagctca aagagctgac cgaaaaggcc gacaaactgg
     5641 ccgctgcggt tgacaaggta gtcggcgaga actctaaaat atgggacatt gtgaagggcc
     5701 ccggcgcagc caagagcact tcatcaagtt cgtcatctgc ggtgagagga gctacgtcgc
     5761 gtggaggcga cccgggggca gacaggggcg aacccgtgat cggctcttcg tcttcagcat
     5821 ggctgggccc tgaagaggag atacgttcta gccccgccca ggacgaggac gagctagctg
     5881 tggctgccag tgaggcgctt gccagactgt ccgagctgac caactccaca cagaaagaca
     5941 tgtatagcct gaggtcatca gcgacacagt atcgcaacaa actcaatcac acgccttcga
     6001 ttgcgcccac aaaagggaag atcacctcgg gctacggcaa caggctccac ccgattctgc
     6061 gggtcaggcg ccttcacgaa ggtgtggaca tagcagcgcc gcggggaaca cccattgtgg
     6121 ccactgctga cggggtcgta gctagggccg gatggctggg cagttacggg aatgttgtcg
     6181 agatttctca cgggtacggc ataaccacgt tctatgcaca caactcgcgc aatgcggtga
     6241 aggttggcgc cactgtgaaa aggggccagg taatcgcata tgttggcagc accgggctga
     6301 gcaccggggc tcatgtgcat tacgaggtgc gcgtcaataa gcgtccggtc gacccgtctt
     6361 cttatatgaa gtagcgaatc aggcagacta cccagtggaa gggacggggg gtcgatgttc
     6421 cgaagagaca ggcagctgtc ttcgggcgac acaaaggtgg atacttacat aggcaaaggc
     6481 gccagactcc agggcactgt gagtgtatcc ggcactgtgc gcgtggatgg tttcgtggat
     6541 ggagtcgtcg aagcggagaa agacgtaata atcggccagg gtggtctggt caccgcatcg
     6601 atcactgctg cgaatgtgac tgtcgccggc gagctgaggg gcaacgtaaa ggcgtcggga
     6661 cggcttgaga ttgcccgcac aggcaaggtt cttggggatg tagctatcgc ttcgtttgtc
     6721 gttgaggaag gagccgcttt cctcggcaag tgcgagatgc tgaaaaccga tgcgcgccat
     6781 ccgaatgcgc atccggatac tatggagaat gaaccaccag gcacagatga gtagcgattc
     6841 aggtttgccg aactggggga cggccgtgct gcgatgcggg gcgccgtccc tcgtggtttt
     6901 ctggatttca cctggttgga ggggtacaag atgcgtttcc gcaaccggat tgaggcaggg
     6961 cggattctgg gcgtagaaat ggccaagggc acttggtccg acccgctagt attggcgctg
     7021 cccagaggcg gggttcccgt tgctgagcat gtagccctcg ccatcggtgc gcaactggac
     7081 gtgatagtgt ctaggaagat aggttcgcct ttcaacccgg aaatgggcat aggcgcggtt
     7141 gggcccgacg gcaacgt
//
LOCUS       DAOTAC010000111         7092 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_8972_length_7092_cov_5.132585, whole genome shotgun sequence.
ACCESSION   DAOTAC010000111 DAOTAC010000000
VERSION     DAOTAC010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7092
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8972_length_7092_cov_5.132585"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(293..2620)
                     /locus_tag="PLM74_06015"
     CDS             complement(293..2620)
                     /locus_tag="PLM74_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HON42675.1"
                     /translation="MGKLGRGRMALALVLFGLCCFVVGSPATAETAGGAAANAPGTGR
                     LELTVLRDGADVTSDYIAVATSMSGAMPVMLAPSQSGALAADLPAGQYMIVVMQPDAM
                     KDPGAMAGQMGQMPSGLPTIVSVAIVAGSTVNKIADVGGGGTFGDPDLFDDFDDFAGF
                     EEPGNGTGDEGESAGPLDLDHIPTADELLELAWRYHDQFWELLSVEEQWMVERMTQRV
                     WLREDLTPTREDVAEVTTAAAAIAAPGASREIPVVLAARAVIAMPESPLAVNNFGAML
                     RLIGKLRDSVTVLLAAKELDSKSPMILTNLANSLYELGDSYAAESLYNGVLAVAGDFG
                     PALTGLGNIYMERKDYKRALEVMLRGAQAGYCGAVKNACSEAIAGEYGSPGSIPPLPP
                     AWKGGIKTAPPAPGGGVGAQSTFTVPQIADWSSIATVAGYMSESGFRKVEAEIRRELS
                     AATAQMQQLAAEIQSVSARRRSSGSALCTISFENQVFHLKLIEDHFKAIVHRSATRAH
                     EEMRWDEAQSDWKEIGERYLHRLEAAKTDAEAEAAHAAFCKEASAFAQAQFTKNKGIW
                     TQFYSEAASAAEDYWAFSRDVLDSIYDPLVYKREEIGRRMIVYGMLAEIAGTGGLLAE
                     LPVIHSGCSRCGGTGRATSDGGSPNAPQSSDKCPFKGGRRFALSLGPVEYKVDCSTVE
                     IGFALIAAGSLKWDFKEKRVTSVFVGVGGQMGVGPASLGTKWGTQLTFDTDGSVSDIT
                     ADWSSSMGVGPVSGELSTDSGLVVGVPGLEATVAR"
     gene            2982..3482
                     /locus_tag="PLM74_06020"
     CDS             2982..3482
                     /locus_tag="PLM74_06020"
                     /inference="COORDINATES: protein motif:HMM:NF019013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HON42676.1"
                     /translation="MLGFRKTSLTLALLLAAVLSVSAGAFANMEAAPSLETTKSVFMS
                     YMESHDIKYATDDVIFFNAALEEPFAIGREALGEALYWFYHVAFDAIAIPRNIVIGEG
                     KAVYEGTIVGTHIGEFAGIPATGNHVAFPIIISYELERTPPYHIRRATIYIMLNILMQ
                     QIAPSN"
     gene            complement(3649..3725)
                     /locus_tag="PLM74_06025"
     tRNA            complement(3649..3725)
                     /locus_tag="PLM74_06025"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3689..3691),aa:Thr,seq:ggt)
     gene            complement(3752..3828)
                     /locus_tag="PLM74_06030"
     tRNA            complement(3752..3828)
                     /locus_tag="PLM74_06030"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3792..3794),aa:Met,seq:cat)
     gene            complement(3846..3921)
                     /locus_tag="PLM74_06035"
     tRNA            complement(3846..3921)
                     /locus_tag="PLM74_06035"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3886..3888),aa:Gly,seq:tcc)
     gene            complement(3932..4017)
                     /locus_tag="PLM74_06040"
     tRNA            complement(3932..4017)
                     /locus_tag="PLM74_06040"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3981..3983),aa:Tyr,seq:gta)
     gene            complement(4037..4111)
                     /locus_tag="PLM74_06045"
     tRNA            complement(4037..4111)
                     /locus_tag="PLM74_06045"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4077..4079),aa:Thr,seq:tgt)
     gene            complement(4181..5062)
                     /locus_tag="PLM74_06050"
     CDS             complement(4181..5062)
                     /locus_tag="PLM74_06050"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HON42677.1"
                     /translation="MQVIESARNSAVRAYKECVARGKPGLVPLEGFRLVEDAMHAGAA
                     LKALYVSEKAAASAAGRQLAELARERGADLIIVSDDVAKRMADTRNPQGVFAIAAWAP
                     QEGARLLAEAAKDDKLSFVVLLDGISDPGNLGTVVRAAAALGAAGVVAGPGCAGFANP
                     KVVRASMGSIFRIPTARVEELEGFVRQARTRDYEIIVSDVRKGISADELAWPGEGAQR
                     AILAVGSEARGVSEAVCRHATAWIKLDMSRDVESLNAASAASILIYILGRNVARWGRD
                     GEVESAQRPKKSPTRAE"
     gene            complement(5064..5426)
                     /gene="rplT"
                     /locus_tag="PLM74_06055"
     CDS             complement(5064..5426)
                     /gene="rplT"
                     /locus_tag="PLM74_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HON42678.1"
                     /translation="MARVKSGIITRRRHNKTLKLAKGYTGVNRISWKRANQAVMHAMD
                     YSYMHRRTKKRDFRKLWIARINAAARTNGISYSRLISGLKKAGVEINRKMLADIAVRD
                     AAAFGQLVAVAREQEAAH"
     gene            complement(5471..5668)
                     /gene="rpmI"
                     /locus_tag="PLM74_06060"
     CDS             complement(5471..5668)
                     /gene="rpmI"
                     /locus_tag="PLM74_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HON42679.1"
                     /translation="MPKMKTHRASAKRFRVTGSGLIIKNKAGRRHDLGKKTSKRKRNM
                     RKADVVAKADAKVVRQLIPYK"
     gene            complement(5714..6172)
                     /gene="infC"
                     /locus_tag="PLM74_06065"
     CDS             complement(5714..6172)
                     /gene="infC"
                     /locus_tag="PLM74_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015757912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HON42680.1"
                     /translation="MDDNGEQLGIMALRDALRAAAERNLDLVEVAPTARPPVCRIMDF
                     GRFKYEQSKRDREARKKQHIVSIKEVRMTPKIEDHDFEVKVKNAEKFLREGDKVKVSV
                     RFRGREIVHTDIAQRLLREMAATLSGVSNMEREPKVEGRNMIMILSPKQD"
     gene            complement(6488..>7092)
                     /locus_tag="PLM74_06070"
     CDS             complement(6488..>7092)
                     /locus_tag="PLM74_06070"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019642483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HON42681.1"
                     /translation="LRDALESNGLAYSIDPGEGVFYGPKIDIKLKDALGRLWQGPTIQ
                     VDFNLPERFDVNYIGEDGEKHRAIMIHRVVLGSMERFTGCLIEQYAGAFPVWIAPVQA
                     KVLSIGEKHVDYARQVAHKLASAGFRVETDLRDEKIGYKIREAQLQKVPYMLIVGEKE
                     MEAGAVSVRARKAGDLGSMSLAEFQGRLASDVESRSCADV"
BASE COUNT         1408 a         2348 c         1821 g         1515 t
ORIGIN      
        1 gactgctgtg gctcagtgat atacccccgg gggtatccac ccagagatac ggtcactttt
       61 tgactgacga tatgccagct ccagccgttt tgtcccatgg cggtcatggt tagaccagct
      121 aaaggacgct ttcgactaag gtgacccctt ctgtcgtcac tatgtctggc aatgcctgcc
      181 cgtattcccg cttcgcgatg cctacaagga ctatacagac cgcgtgcccg tttctgcctc
      241 atcatccctg ccgcatcatc cggatcagcg gcacccgctc ccaatcctcc gcctacctag
      301 ccactgtcgc ctccaacccc ggtactccta cgaccaagcc tgagtcggtg ctcaactccc
      361 ccgacaccgg gccgaccccc atggaacttg accaatcggc agtgatgtct gacacggaac
      421 catcggtatc gaacgtcagc tgggttcccc atttggtccc gagcgaggct gggcccacac
      481 ccatctgccc gcccacgccc acgaacacgc tggtaacgcg cttctccttg aagtcccact
      541 tgagcgaacc ggcagcgatg agcgcgaaac cgatctccac tgtggaacag tccaccttgt
      601 actccacagg gccaaggctc agtgcgaacc tccgcccacc cttgaatggg cacttgtcgc
      661 tgctctgagg tgcattcggc gagcctccat cacttgtcgc ccgccccgtc ccgccgcatc
      721 tactgcaacc gctgtgaatc acgggtagct cggccagcag cccgccggtg cccgctatct
      781 ccgccaacat accgtacaca atcatcctgc gtccaatctc ttcgcgcttg tataccaacg
      841 gatcgtatat ggagtcgagc acgtcccgag agaaagccca gtagtcctcg gcagcagaag
      901 cagcctcgct gtagaactgc gtccatatgc ccttgttctt tgtgaactgc gcctgcgcga
      961 aagcgctcgc ttccttgcag aaagctgcgt gagccgcctc ggcctccgca tcggtctttg
     1021 cagcctccag acgatgaaga tacctctccc cgatctcctt ccagtcgctc tgtgcctcat
     1081 cccaccgcat ttcctcatga gcgcgcgtcg cgcttcggtg gacaatggcc ttgaagtgat
     1141 cttctatcag tttcaaatga aacacctggt tctcgaaact gatcgtgcat agagcactgc
     1201 cggagctccg cctccgggcg ctcacactct gtatctcggc cgcgagttgc tgcatctgcg
     1261 ctgtggccgc cgaaagctcg cgtcggatct cggcctcgac cttccgaaac cccgattcgc
     1321 tcatgtaacc ggcgacggtc gctatcgaac tccagtcggc aatctgaggc acagtgaatg
     1381 tggactgagc cccgactcct cccccgggcg ccggcggcgc cgtcttgatg ccgcccttcc
     1441 acgccggagg cagcggcgga atgctgcccg gactgccgta ctctcccgct atcgcctccg
     1501 agcaggcatt cttcaccgca ccgcaatacc ccgcctgcgc ccccctgagc atgacctcca
     1561 atgcccgctt gtagtccttg cgctccatat agatgttacc gagaccggtg agagccgggc
     1621 cgaaatcccc ggccactgca agcacgccat tgtacaaaga ctcagccgca taggagtcgc
     1681 ccagctcata cagggagttc gccagatttg tgagtatcat cggagacttg gagtcaagct
     1741 ccttcgcggc cagtagcacg gtgaccgaat cgcgcagctt gcctatgaga cggagcatcg
     1801 ctccgaagtt gttcaccgcc agcggagact cgggcatagc gatgacagcc cgcgccgcca
     1861 gtaccaccgg gatctcccgt gacgccccag gcgccgcaat cgcggccgcc gccgtggtta
     1921 cttccgcaac atcttcgcgc gtgggagtca aatcctcccg gagccatacg cgctgagtca
     1981 tgcgctcaac catccactgt tcctcgacac tgagcagttc ccagaactgg tcatgatacc
     2041 tccatgccag ctcaagaagc tcgtcagcag tgggtatgtg gtccagatcc aacgggccgg
     2101 cactttcgcc ttcatcccca gtaccgtttc cgggctcttc gaaaccggcg aagtcgtcga
     2161 agtcgtcaaa aaggtccgga tcgccgaacg tgcccccgcc gccgacatcg gcgatcttgt
     2221 tcacggtgct gccggctacg atcgctacgc ttactatcgt gggcagtccc gacggcatct
     2281 gccccatctg ccccgccatc gccccgggat ccttcatggc gtctggctgc attacgacga
     2341 tcatgtactg tcctgcgggc aggtcggccg ctagggcacc cgactgcgaa ggcgccagca
     2401 tcaccggcat cgcccccgac atgctcgtcg ccactgctat gtagtcggag gtcacgtccg
     2461 ccccatcgcg caaaacggtc aactccaagc ggcctgttcc aggcgcattc gccgccgccc
     2521 cgcccgcagt ctccgctgtg gccgggctgc cgaccacaaa gcagcacaac ccgaatagca
     2581 caagcgcgag ggccatcctg cccctgccca gcttccccat gcagatatcc tccgtttcag
     2641 caacctctct ggtaattccg cctggtttcg aatgttctgc aggtcagcct caatccctcc
     2701 acacatgcgt gatgtactcg cggcgttttt ctttcatatc cgtgtcgact ccactgtccg
     2761 ctcggatcgg gatttggagc cagtgttgtt cgcaaatcac ggattcaggg cggcaagccg
     2821 cgctacaaca tcgtgcctat gccggctcca tcgggctatc tttcggaagt tctggtttga
     2881 catgtggtct gctattgccg cactatggtt acagagggag gtgaagagtg tcgcaaccag
     2941 gtgtgcatga ccttacctgc gcaagaaggg gtggatacac gatgttgggg ttcagaaaaa
     3001 cgagtctgac cttagccttg cttcttgcag ccgtattatc agtcagtgct ggagcattcg
     3061 ccaacatgga agcggcgccg agcttggaga ctaccaagtc ggtgttcatg tcgtacatgg
     3121 aatcgcatga catcaagtac gctaccgatg atgtgatatt cttcaacgcg gcgctggagg
     3181 agccattcgc aatagggcgg gaagcactgg gggaggctct gtactggttc tatcacgtgg
     3241 cctttgacgc aatagcgatt ccaaggaaca tagtcatcgg cgagggcaag gcagtgtatg
     3301 agggcacaat cgtagggacc cacattggtg aattcgctgg aatcccagcc actggcaatc
     3361 acgtggcgtt tcccatcatc atcagctacg agcttgagag gacaccaccc taccacatca
     3421 ggcgagctac catctacata atgctgaaca tcctgatgca gcaaatcgcc ccgagtaact
     3481 agaagccaga gtgaaccgct tcctgtcaga taggcagtgg ggataacgag actgcgcagg
     3541 ggcgccaatc ttgatggtcg atgtctctgt tgcgcagcca tgcctgaaag ccatgcctaa
     3601 aggcagaaag ctctctgcac caacgcgtgc agagagcttc ctaattcctg gagcccgcga
     3661 tcggaatcga accgatgacc tcatccttac catggatgcg ctgctaccta ctgagctacg
     3721 cgggcgggcg ccctgtgcgg gtcgcctaaa atggttgcgg gggcaggatt tgaacctgcg
     3781 acctccgggt tatgagcccg acgagctacc agactgctcc accccgcgac aaacttatca
     3841 atctctggag cgggaaacgg ggctcgaacc cgcgacattc agcttggaag gctgacgctc
     3901 tgccaactga gctattcccg caaggatatc atggtggaga gaggaggatt cgaacctccg
     3961 aacccgttcg ggaacagatt tacagtctgt cgcctttaac cactcggcca tctctccgcg
     4021 caaacactac gaaaactgga gccgacgatg ggaatcgaac ccgcaacctg ctgattacaa
     4081 gtcagccgct ctaccattga gctacgtcgg cggtcaatcg caccgtgcag aagttaggat
     4141 accatgttag ctgccttaag tcaaccactt attcggccgc ttattcagcc cttgttgggc
     4201 ttttcttcgg cctctgtgct gattcgactt cgccgtctct gccccaccgg gccacattcc
     4261 tgcccagaat gtagatgagg attgaggccg cagaagccgc gttgagcgac tccacatcgc
     4321 gcgacatgtc gagcttgatc caggcggtgg cgtgcctgca cacggcctcc gaaacacccc
     4381 gagcttcgga ccccaccgcg agaatggccc gctgtgcgcc ctctcccggc cacgccagtt
     4441 catctgcgct tatccccttg cgaacgtcgg acacaatgat ctcgtagtcg cgcgtccgcg
     4501 cctgcctgac gaagccttcc agctcttcaa ccctggctgt ggggatcctg aagatcgatc
     4561 ccatcgacgc ccttacgacc ttggggttgg cgaaaccggc gcaaccaggg cccgccacaa
     4621 cacccgctgc acccagcgca gccgcggcgc gcacaacggt gccgagatta cccgggtcgg
     4681 atatgccgtc aagcaaaacg acaaaggaca acttatcatc ctttgccgcc tccgcgagta
     4741 atcttgcccc ttcctgcggc gcccacgcag cgatcgcaaa gacgccctgc gggttacgag
     4801 tatcggccat ccttttcgca acgtcatcag agacgatgat caggtctgcg ccgcgctcgc
     4861 gtgccaattc cgcaagttgc cgccctgccg ccgaggcggc agccttctcg gacacataca
     4921 gcgccttaag cgccgcgccc gcgtgcatag catcttccac aagacggaat ccctcaagag
     4981 gaactagccc gggcttgccc cgggccacac actctttgta ggcgcgcact gcagagttcc
     5041 tggcgctttc gattacctgc aactcaatgc gccgcttcct gctccctggc cactgccaca
     5101 agctgcccga aggcggcagc gtcgcgcaca gcgatatcgg ccagcatctt gcggttaatc
     5161 tcaaccccgg ccttcttcag cccggagatc agcctgctat aggagatgcc atttgtgcgc
     5221 gcagcagcgt taatgcgcgc gatccaaagc ttccgaaagt cgcgcttctt ggtccttcga
     5281 tgcatgtagg aatagtccat ggcatgcata acagcctgat tagcgcgctt ccacgaaatc
     5341 ctgtttacac ccgtatagcc cttcgcaagc ttgagagtct tgttatgcct gcgtctggtg
     5401 ataattccgc tcttcaccct tgccattgct gttcagctcc ctccggccat catcacgcta
     5461 catccgcgcg ttacttgtac ggaatcaact gacggaccac tttcgcgtca gccttggcga
     5521 ccacatccgc cttcctcatg ttccgtttgc gcttagacgt cttcttcccc agatcgtgcc
     5581 tgcggccggc cttgttcttg atgatcagac ctgagccagt cacgcgaaac ctcttcgcgg
     5641 acgctctgtg cgttttcatc ttaggcatac cgattgccct cctcaaccaa ctgaatccca
     5701 accaaaccgg cttttagtcc tgctttggag acaggatcat tatcatgttc ctgccttcga
     5761 ctttcggttc acgctccatg ttgctaactc cggacagggt tgcggccatc tctctcagga
     5821 gcctctgcgc gatatctgtg tggactattt cccgcccgcg aaacctgacg cttaccttga
     5881 ccttgtcgcc ctcacgcagg aacttctctg cgttcttgac tttcacttcg aagtcatgat
     5941 cctcgatctt gggcgtcatg cggacttcct tgatgctcac aatgtgctgc ttcttcctgg
     6001 cttctcgatc acgcttgctt tgctcgtact taaacctacc gaagtccatt atgcggcata
     6061 cgggcggtcg cgctgtaggt gcaacctcga cgaggtccag gtttctctcc gccgctgcac
     6121 gaagggcatc cctcaaggcc attatgccca gttgctcccc gttatcgtcc acaacgcgga
     6181 cttccctggc tctgatctcc tcgttgacga aaagctccct actgatagcc tgtcaccccc
     6241 aacggtttta tggatactgc caaaaacaga gcgtgcacta tgcaaaatgg gcgggtgttc
     6301 cacccgcccg acatgacaga ttagccgaaa tccgacgaca aatcgaagtc ggactcgcga
     6361 tcagtcagca taaccggcct ggctgaagcg cctagcgcaa cgccggacgg tgagaagcgg
     6421 atggcttctg cttcaacacg tgttctgatt ttgcaccaca gagtatagca caacggcgca
     6481 aggtccgtca aacatcagcg cacgagcgag actcgacgtc agacgccagt ctgccctgga
     6541 attcggcaag gctcatggag ccaagatcgc cggccttgcg agcccgaacc gatactgcgc
     6601 ccgcctccat ctccttttcg cccactatca gcatataggg caccttctgc agctgtgcct
     6661 cgcggatctt gtagccgatc ttctcgtcac gcaggtccgt ctccaccctg aagccggcag
     6721 acgccagctt gtgggcaacc tgccttgcgt agtcaacgtg cttctctccg atggagagca
     6781 ccttggcctg cacaggcgcg atccatacag ggaaggcgcc cgcatattgc tctatcaagc
     6841 agccggtgaa ccgctccatg ctgcctagca ctactcggtg gatcatgatc gccctgtgct
     6901 tctcgccgtc ctcaccgata tagttcacat cgaacctctc aggcaggttg aagtctacct
     6961 gaatcgtggg accctgccac aacctgccca gagcgtcttt gagcttgata tcgatcttgg
     7021 gcccgtagaa cacgccctcg ccggggtcga ttgagtaggc caggccattg ctctccagcg
     7081 catctcgcag tg
//
LOCUS       DAOTAC010000112         7083 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_8981_length_7083_cov_5.114400, whole genome shotgun sequence.
ACCESSION   DAOTAC010000112 DAOTAC010000000
VERSION     DAOTAC010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7083
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8981_length_7083_cov_5.114400"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="PLM74_06075"
     CDS             complement(<1..835)
                     /locus_tag="PLM74_06075"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HON42682.1"
                     /translation="MRFITGVNYWPRNHGVQMWAEFDREEIAGDMRTIARIGMDTVRV
                     FIKWADFQPAPDIIDDTMVRRFDELLVMADEAGVRVIPTFFCGHMSGENWDVPWRGGR
                     DPYSDPEMLRAQVRLVGHFAQRYRGDVRILCWDLANEQDIFARPRDRHSGWLWIRTLA
                     SELKLHDPEHPVTAGTHITSLTEDCGFRMDDLAESLDFLCMHAYPLYADSCPERLDGV
                     RNTYFVPFCAALTRTLGRGADVLFEEFGATSQMCAEDVIEGYYRATLHALLSEGVIGA
                     MG"
     gene            complement(832..1773)
                     /locus_tag="PLM74_06080"
     CDS             complement(832..1773)
                     /locus_tag="PLM74_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42683.1"
                     /translation="MSTTRSVDSKRGAAQVAPILKVLCQALCQALSKATCVTLATCAL
                     CVLVCPAVPAFAATQAQPGVAYAESYFEYRSDTYCIGISLDPVFIDYLCADLGGDGEF
                     RELLAYVGKPSSLWLGVGGWGTHKPGKPAQAEVREEEDGRVLLALRDIEFVGFLPTNT
                     GHIARVRADWVFEFHDDFFDMTVTWRLLDDIAGLQEAGWALNVDVPWYGDSEEARRVN
                     RDVQGFPRWILWYGPDIGVAAAFREGSTQLTANRWYGNLESSYVIVHNWWKPGGSAVG
                     WGTYHGGTWRVGFFRGEHDIGAAERIYSIINAEDGQK"
     gene            complement(1776..3152)
                     /locus_tag="PLM74_06085"
     CDS             complement(1776..3152)
                     /locus_tag="PLM74_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42684.1"
                     /translation="MRTKARGLLAALAVVAMVITMAAGASAATKSAVIDVKSVTGVPV
                     PVSFTMNQIREAIGVDFAANWDSLRVEDSAGNLIPFQLDDVDLSGGVSRVDELAFLAA
                     GPVKIVVSDEAPEAKPSFDAAFEVEPAETGGFVVRALDGLLIANVSKYATVDLVSFRG
                     AEHKYARDIGMIRYAGFPYSTYWYDQNLDRHEEKTTFEEPVRIVKSAALPTGPVRATV
                     VAQTASDLFPGLRQSVVVSIYKTGEVRVNNRLVTAGYSDLTKLFTMCGGVMGDVEDAR
                     HILPVFRWTDWADELGMKSEEYWAELGVITRVDGQPYITFADARGPKPAWWGASYLFC
                     SPERWRTNFSAGLGVGVAECLMDVPEVPADLVDKLKMDHWHLEGEWRTGPFRWIAGEI
                     VDFRAKSGIKVDIIPDMNEGDWPVHMIPGDSVERVNYYLLYEAPDAPSAIRYLESRYA
                     ELAGVVIK"
     gene            complement(3216..5300)
                     /locus_tag="PLM74_06090"
     CDS             complement(3216..5300)
                     /locus_tag="PLM74_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42685.1"
                     /translation="MRIMGYPRALTPVLRVVMAAAIVVLCVVSAYAPTPTLAASQPSA
                     IDARRVVGDMINLAHLDYLRDEISLSDGTIIPIWWVYCEPTVSGDRNSKYRYVEAASE
                     GVSCVDDVARAALAYLADYERTGDPHDLDMARDAFSFIEYMRTPEGHFYNFVLESGAR
                     NLKGSTSEKGVNWWTARAMWALARGVRVFGRAEPGSGYAEHLEALMGPSLGAVHAFLT
                     DGDPALFGQYKTLHGTTIPAWFVGDGADASGVVVMALCELYQARPEPRTAEMIRMFAD
                     GIAQFQMGTADESPFGAHMPWGGSISHWHGWGYHQIRALAMAGRVLGNQEWVRSAERA
                     ANGFVTHMLASIGLFAHLGPAPLTYVQLAYGCQTGTTGLLELYRATGREIYAILAGIS
                     GSWLLGNNVAGHPMYDAATGRGWDGIDPPGPERGIGVSFNAGAESTIEAVTTLIELAG
                     VPRACEYMNLVTRARYPFRVVEAESFDKPASGRPRKMWASWTGEGIPSGEFYVTARSG
                     DSFKLSFSIPEDDEFIPYVVYERQAVALGQVSLAISIDDGEPIIVDASGSPDTKYFVM
                     EKLCDLVWLTAGRHSLTVKFAGASKSLNASIDALVFQPVVEWRHMTGQDYQNVLLTRS
                     FAGQAVTNQINVDIRKSAPAPTIEFLVRCYTAQGELAREVRLTSPVTSGAETALLDLP
                     VEPFGYSLVEWR"
     gene            complement(5342..6673)
                     /locus_tag="PLM74_06095"
     CDS             complement(5342..6673)
                     /locus_tag="PLM74_06095"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HON42686.1"
                     /translation="MTRPRFALMSRILAFVAACLLVAGLPPRAAIWAAVGVRAPVQLD
                     FWCSSNPYEIEFARAVVDEWNLARSDIKVRLQPLPASRSTEEALLAAIAARTTPDLCA
                     NIYPAVVTQFVAAGGLYRMDSFPDFVSYMRGRLPDGILEQYRSPDGHYYQAPWKSNPL
                     MLAYNAGMLRAAGVDPSSLASYSSFLAAAAKLTRDTNRDGRTDQWMIYLNIEPIWWQR
                     FFDFYTLYAAASGGRTLIESNRAAFNNNAGVAVMQFLSDLFLRGYAPKSAFPGDVFLQ
                     QKVATVITGPFAVPFYESMKPKGFEYDFVPVPVPDSFRGREVWTYGDPKNIGIFATSK
                     YPKEAWEFAKFILSAENDALFLEMTSQIPYRRNLEASPAYRSLLRKQPVLARFVRQAE
                     FTCGADNVRPLVEIYDAIAQEYQYCAVLGQESARSAMEIAEEKVNEILSDY"
     gene            complement(6688..>7083)
                     /locus_tag="PLM74_06100"
     CDS             complement(6688..>7083)
                     /locus_tag="PLM74_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HON42687.1"
                     /translation="GMRGPVITFGDPKNIAIFATSKHPLEAWEFVKFIMSRENDLLWL
                     EITSQPPYRRDLAKDPMFAGYFKENPLMAKFVEQGVYTRGVDDSKYLIEIFDAISKQY
                     DLCCVQGKKTPKIAVRDAAQAVRDILAGW"
BASE COUNT         1430 a         2300 c         1972 g         1381 t
ORIGIN      
        1 accccatcgc cccgatgacg ccctccgaga gcagcgcgtg cagcgtggcg cggtagtacc
       61 cctcgatgac gtcttcggcg cacatctggc tggtggcgcc gaattcctcg aagagcacgt
      121 cggccccccg gcccaatgtc cgcgtaaggg cagcgcaaaa cggcacgaaa tacgtgttcc
      181 tgaccccatc caggcgctcc gggcacgagt cggcgtacag ggggtaggcg tgcatgcaca
      241 ggaaatccag ggattccgct aggtcgtcca tgcggaagcc gcaatcctca gtaaggctgg
      301 ttatgtgggt gccggccgtg acaggatgct ccgggtcatg caacttcagc tcggatgcga
      361 gggttcggat ccaaagccac ccagagtgcc gatctctcgg acgcgcgaat atgtcctgct
      421 cgttcgcgag gtcccaacac agaatcctga catcgcctcg atacctctgc gcgaaatgcc
      481 cgaccaacct aacctgtgct ctcagcatct ccgggtcaga ataggggtcg cgcccgcctc
      541 tccatggcac gtcccagttc tctccgctca tatgcccaca gaagaacgta ggaatgaccc
      601 tcacccctgc ctcatccgcc atgacgagga gttcgtcgaa ccggcgaacc attgtatcgt
      661 cgatgatgtc gggcgcgggc tggaagtctg cccattttat gaagaccctg actgtgtcca
      721 tccctatgcg cgcgatagtg cgcatgtccc ctgctatctc ttctctatca aattcggccc
      781 acatctggac cccgtgattc cgcggccagt agttgacgcc ggttatgaat ctcatttctg
      841 tccatcctct gcattaatga tgctgtagat ccgttcggct gcgccgatat catgctcgcc
      901 tcggaaaaac cccacacgcc atgtgccccc gtgatacgtg ccccagccca cggctgaccc
      961 gcccggcttc caccagttgt gcacaatcac gtagctggac tctaggttcc cgtaccatcg
     1021 gttggccgta agctgcgtgg agccttcccg aaacgccgca gccactccaa tgtcgggtcc
     1081 gtaccacagt atccaccgcg ggaacccctg cacatcgcgg ttcacgcggc gcgcctcttc
     1141 agaatcaccg taccagggga cgtccacatt cagcgcccag ccggcttcct gaagtccagc
     1201 aatgtcatct agcaggcgcc aagtgacagt catgtcgaag aagtcatcat ggaactcaaa
     1261 tacccaatcc gcgcggacgc gagcaatatg gccagtgttg gtgggcagaa agcccacgaa
     1321 ctctatgtcg cgcagcgcca acagaacccg accgtcttct tcctcgcgca cctcggcctg
     1381 cgccggctta ccaggcttgt gagttcccca tcctcccacg cccaaccaaa gcgacgacgg
     1441 cttccccacg taggccagga gttcacggaa ctcgccatcg ccgcccaggt cagcgcagag
     1501 gtagtcgatg aacacagggt cgagtgatat tcctatgcag taagtgtccg accggtactc
     1561 gaaataggac tcggcatacg caaccccagg ctgagcctga gtcgccgcga atgcaggaac
     1621 cgcagggcag accaggacgc agagcgcgca ggtcgcaagt gtaacacagg tcgccttgga
     1681 gagggcctgg caaagagcct ggcaaagaac cttgaggatg ggcgcgactt gcgccgcgcc
     1741 ccgcttcgaa tccactgaac gagttgtcga catggttact tgatcaccac gccagcaagc
     1801 tcggcatacc tgctctcaag ataccgaatg gccgacggcg cgtccggcgc ttcatacaac
     1861 agatagtaat tgacgcgctc cacgctgtcg cctgggatca tgtgaacagg ccagtctcct
     1921 tcattcatgt cggggatgat gtcgacctta atgccgctct tcgcgcggaa atccacgatc
     1981 tcgcctgcga tccagcggaa ggggccggtt cgccactcac cctcgagatg ccagtgatcc
     2041 atcttgagct tgtcgactag gtcggcgggt acctccggca cgtccatgag gcactcagct
     2101 acgcctactc cgaggccggc ggagaagttg gtgcgccacc gctccgggct gcagaacagg
     2161 tatgacgctc cccaccatgc aggcttggga ccgcgggcat cggcgaatgt tatgtagggt
     2221 tgcccatcca ctcgggtgat cacaccgagc tcagcccagt attcctcgga cttcatccca
     2281 agctcgtctg cccaatccgt ccagcgaaag acgggcagga tgtgccgtgc gtcctcaaca
     2341 tcgcccatca cgccgccgca catcgtaaaa agcttggtca ggtcggagta gcccgccgtg
     2401 acgaggcggt tgttcaccct tacctcgccg gtcttataga tggagacaac tactgactgg
     2461 cgcaaccccg ggaagagatc ggacgccgtc tgggccacaa ccgtagcacg aacggggcct
     2521 gtgggcaatg cggcagactt gactatcctg acaggctctt cgaatgtggt cttttcctcg
     2581 tgacggtcca ggttctggtc gtaccagtag gtgctgtagg ggaaacccgc gtatctgatc
     2641 atgccgatat cgcgcgcgta tttgtgctcc gcacccctaa agctgaccag atccacggtg
     2701 gcatacttgc ttacgttagc gattagcagt ccatccaagg cccggacaac gaacccgccc
     2761 gtctccgcgg gttccacctc aaaggccgca tcgaaagagg gtttagcctc gggcgcctcg
     2821 tccgaaacga cgatcttcac cggccctgca gccagaaatg ccagttcatc gacgcgcgag
     2881 acaccgcccg acaggtcaac atcgtccagc tggaagggga tcaggttgcc tgccgagtcc
     2941 tccactctga gcgaatccca gttggccgcg aagtccactc caatggcctc tcgaatctgg
     3001 ttcattgtga acgacacggg cacaggaaca cctgtgaccg acttgacatc gatcactgcc
     3061 gacttggtgg cggcagacgc cccggctgcc atggtgatca ccatcgccac cactgccagc
     3121 gccgccagaa gacccctagc cttggttctc atctcgaatt ctcctccttg tcgccatatt
     3181 cagtgtgctc tcggttttcc cgacagttca cgccgttatc tccactccac cagcgagtag
     3241 ccgaaaggct ccacaggcaa gtccaacaga gccgtctcgg cccccgacgt caccgggcta
     3301 gtcagccgca cctcccgtgc gagctcgcct tgggcggtgt agcagcgcac caggaactcg
     3361 atcgtcggcg ccggagcgga tttcctgatg tctacattaa tctgattcgt cacagcctgc
     3421 ccggcaaagc tcctggtaag cagaacattc tgatagtctt gcccagtcat gtgcctccac
     3481 tcaacaacgg gctggaacac taaagcatcg atagacgcgt tcaggctctt gctcgcaccg
     3541 gcgaacttca cggtcaagct atgccgcccg gcggtcagcc atacgaggtc gcagagcttc
     3601 tccatgacga agtacttggt atccggcgaa ccgctcgcgt cgacgatgat gggttcgcca
     3661 tcgtctatcg atatggccag gctcacctga cctagggcca ccgcctgccg ttcatagacg
     3721 acatagggaa tgaactcatc gtcttccgga attgagaagc tcaacttgaa gctgtcgccg
     3781 gaccttgctg tcacgtagaa ctcgccgctc gggatgccct cccctgtcca gctggcccac
     3841 atcttgcgtg gcctgcccga tgcgggtttg tcgaacgact cagcctccac gactctgaag
     3901 ggatagcggg cccgcgttac aaggttcatg tattcgcacg ctctgggcac gcccgcgagc
     3961 tcaatcaggg tagtcaccgc ctcgatcgtg gattcggcgc ctgcgttgaa gctcacgcct
     4021 atgcctcgct ccggacccgg gggatcgatg ccatcccagc cgcgacccgt ggcagcatcg
     4081 tacatgggat gcccagccac gttgttgccg agaagccagg aaccggatat gcctgcaagg
     4141 attgcgtaaa tctcgcggcc tgtagctcga tacagctcca ggaggccagt ggtaccggtc
     4201 tgacagccat aagccagctg gacgtaggta agcggcgccg ggcccaagtg agcgaaaagg
     4261 ccgatcgagg ccaacatatg agtgacgaac ccgttcgcgg cgcgctccgc cgaccgcacc
     4321 cactcctggt ttccgagcac cctgccggcc atggcaagcg cgcggatttg gtggtagccc
     4381 caaccgtgcc agtggctgat cgatcctccc catggcatgt gcgctccgaa cggggactcg
     4441 tctgcggtgc ccatctggaa ctgggcgatc ccgtcggcga acatccgtat catctctgcg
     4501 gtacgtggct ctggccgcgc ctgatacagc tcgcacaagg ccatcaccac gacgccggac
     4561 gcatccgcgc cgtcgcccac gaaccaggcg ggaatggtcg tgccgtgcag agtcttgtac
     4621 tgcccgaaca gagcgggatc cccgtccgtc aggaaggcat gcacagcacc gagcgacggc
     4681 cccatcagtg cctccaggtg ttcagcgtag cccgaacccg gctccgcccg gccgaacacc
     4741 cgcacaccgc gagccagggc ccacatggca cgtgccgtcc accagttgac tcccttttcg
     4801 ctggtagatc ccttgaggtt gcgggcgcca ctttccaata cgaagttgta gaagtgccct
     4861 tcaggcgtcc gcatgtattc gatgaagctg aacgcatccc gggccatgtc gagatcgtgc
     4921 ggatcgcctg tgcgctcgta gtctgcaagg tacgccagcg cagcgcgggc aacgtcatcc
     4981 acacagctca caccctcact ggcggcctcg acgtacctgt acttggagtt tctgtcgccg
     5041 ctcacggtcg gctcgcagta tacccaccat atggggatga tggtcccatc tgaaagactg
     5101 atctcgtctc tgagataatc gaggtgagcg aggttaatca tgtcgccaac cacccggcgc
     5161 gcgtcaatcg ctgatggctg actggcagcg agtgtcggcg ttggagcgta ggccgaaacg
     5221 acgcacagaa ccacgatcgc tgccgccatg actacgcgca ggaccggtgt caatgcgcgc
     5281 gggtaaccca tgatacgcat cgtttgatcc tccaactctg cgcaaagtcg cgatggacat
     5341 cctaatagtc gctgagtatc tcgttgacct tctcctcggc gatctccatg gcggacctcg
     5401 ccgattcctg tccgagcacg gcgcagtact ggtactcctg agcgatagcg tcgtagatct
     5461 ctacgagcgg ccggacgttg tcggcgccgc acgtgaactc cgcctggcgg acgaagcgcg
     5521 ccagcacagg ctgttttcgc agcagcgatc tgtaggcagg cgacgcctca agattccgcc
     5581 tgtatggtat ctggctggtc atctctagga atagtgcgtc attctccgcg ctcaagatga
     5641 attttgcgaa ttcccaggct tctttcggat acttgctcgt cgcgaatatt ccgatgtttt
     5701 tggggtcgcc ataggtccac acttcccggc cgcggaagct gtcaggtaca ggcaccggca
     5761 caaagtcgta ctcgaaccct ttgggcttca tcgattcata aaagggaacc gcaaacggcc
     5821 ctgttatgac agtcgccacc ttctgctgca ggaacacatc gcccggaaac gccgacttag
     5881 gagcgtaacc ccgcaggaac aggtccgaca gaaattgcat caccgcgact ccggcgttgt
     5941 tgttgaaggc cgccctgttg ctctcgatca acgtccgccc accactcgcc gcggcgtaca
     6001 gtgtgtagaa gtcgaagaac ctctgccacc atatgggctc tatgttgagg tagatcatcc
     6061 actgatccgt cctgccgtcg cggttggtgt cgcgcgtgag cttcgccgcc gcagccagaa
     6121 agctgctgta gctggccaaa gatgagggat cgacgcctgc ggcccgaagc atgcccgcat
     6181 tgtaagccag catcaggggg ttggacttcc acggcgcctg atagtagtgg ccgtcgggcg
     6241 acctgtactg ctccaggatt ccatcgggca ggcgtccgcg catgtagctt acgaaatccg
     6301 ggaagctgtc catgcgatac agcccgcccg ccgcgacgaa ctgcgtcaca acggcagggt
     6361 atatgtttgc gcacagatcc ggcgtcgtgc gtgccgcgat cgccgcaagc agggcttcct
     6421 ctgtggatct cgacgcaggc agcggttgca gtctcacttt gatgtccgac ctcgctagat
     6481 tccactcatc caccaccgct cgtgcgaact cgatctcgta cgggttggac gaacaccaaa
     6541 agtccagctg taccggggct ctcaccccga ccgccgccca gatggccgcc cgaggcggca
     6601 ggcccgcaac gagcaggcat gccgccacga acgccagaat ccgtgacatg agcgcaaaac
     6661 gcggcctcgt catcgacatt gatgctccta ccaccctgct aagatgtcgc gaaccgcctg
     6721 agctgcgtcg cgtacagcga tcttgggcgt cttcttgccc tgaacacagc agagatcata
     6781 ctgcttggag atcgcgtcga atatctcgat caagtactta ctatcgtcca cgccacgagt
     6841 gtacacgcct tgctccacga acttggccat caggggattc tccttgaagt aacctgcgaa
     6901 catcggatcc ttcgcgagat cgcggcgata cggcggttgg ctagtgatct ccagccacag
     6961 caagtcgttc tcgcgagaca tgatgaactt cacgaactcc catgcctcca gcggatgctt
     7021 gctagttgcg aagatggcta tgttcttggg atcgccgaaa gtgatgaccg gtccacgcat
     7081 gcc
//
LOCUS       DAOTAC010000113         7080 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_8987_length_7080_cov_4.858932, whole genome shotgun sequence.
ACCESSION   DAOTAC010000113 DAOTAC010000000
VERSION     DAOTAC010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7080
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8987_length_7080_cov_4.858932"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="PLM74_06105"
     CDS             complement(<1..730)
                     /locus_tag="PLM74_06105"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HON42688.1"
                     /translation="MMRSMFAGVSGTRAHQVRMDVIGNNIANVNTVGYKYGRATFQDM
                     LSQTLRGSMAPTDNRGGVDPMQVGLGTMLRSIDIVFTPGNLESTGKLTDMAIQGNGFF
                     VVRDSAGGLLFTRDGAFKIDAKGNLVHPSTGYQIQGWKADSGGKIDRSLPIEAIAIPL
                     GTSMSAQPTQNVSFVGNLQSGSAAGDKYATSVPVYDSQGLQHTVTMEFTKTATMNEWE
                     WEATSGTPPSAVGRGVVLFDANGAV"
     gene            complement(819..1238)
                     /locus_tag="PLM74_06110"
     CDS             complement(819..1238)
                     /locus_tag="PLM74_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02530 family flagellar biosynthesis protein"
                     /protein_id="HON42689.1"
                     /translation="MTTDKIDRLNSVPVDSRPIRPARPVQPGTAPSLGAAGSAPGTTF
                     EEMLAGELASPALLRFSAHAQARITARRMNLGDEELSRLAVGVNKAAAKGARESLVLV
                     DDKAFVVSIRNRTVITAVDGENMKENVFTNIDSAVIV"
     gene            complement(1251..1655)
                     /locus_tag="PLM74_06115"
     CDS             complement(1251..1655)
                     /locus_tag="PLM74_06115"
                     /inference="COORDINATES: protein motif:HMM:NF015896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HON42690.1"
                     /translation="MYTSRVAGVATYDAAVSEAPRRSLGKDEFLQLLVTQLRNLDIGE
                     GASNQEFIAQMAQFTMVEELQNLSATVTEMMSRQMIAEAGGLVGKTIEAWPPGYTTPI
                     TGRVSAVEVIAGLPFLKVGDVRVSMNHVTKIS"
     gene            complement(1682..4453)
                     /locus_tag="PLM74_06120"
     CDS             complement(1682..4453)
                     /locus_tag="PLM74_06120"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HON42691.1"
                     /translation="MDATTALLVQGCACAPAAHAAVTAAMTSATNLTAGAPTICDAGP
                     DVEFDTLLEQMAGQLQTPEPRYGAEPTVAAEASKRNGHGARVTCADPELDMQPAQDDD
                     GNDDGDEDVDGPVTAAYQQMAMMAAGSAGTAPVQTRAPRHPTPDRTDNRATGQVEDSR
                     SGSLMDEIAMQENASVSAERLAEGRSVDEQDRMSQQSGSRSTVQSSAGRLDGQVDGSR
                     NRLRASELVGRDERMPNGSPRSMNGPVTESHVVPQVQERTAAAAQKRETAGPERRTPE
                     GAHMAVDHMNDLTAGQVDGTQSTTLAAEMPVREHVVMSMERLIGSTGRTVTQVEPKNV
                     HTAAPVERDAPVLNDSSRSIDGSVTEPDVVPQVQEHTVAVIQERSTTRPVTADPEQLA
                     EGRSAGKQDRVSQQPGDHTPVPVHGRASQPEGAHMVADLVNDPTVGQVDCTTSGPRAG
                     EAPVSGSTSASMAQPKGDAGHTAIRVESKNVHTAAPVERDEPVLNGSSRPMDGPVTEP
                     DVVPQVQEHTVAVVRERSMIRPEGRSTVLTDIRGTADADGNTWVVRERPAVIETEELV
                     TTELERYTPARAEGRAPIGESAPVIEPGGIMPEMVDLADGKADMGDTAEPRQTALRPI
                     APRKEGTMTASRVVTGSRGWPEALVRSALVESLARHRNLPNPLWDEGSEKVKQAQSQP
                     EQKYSVSSTSDHAEHREPAPVARPELAVAGDPVVAAAPVEGHDVSQTSQAGRPVLTEQ
                     AGSSVMRQIASHVSARAAQGGGVFRARLVPEHLGSVEVRVRVVRGICTADIRADDAEI
                     ARFIESHSSDLKVSLAEHGVDLAGLSVSSRSRAQEGWSAAVPAHFESDWSGSSWNGAN
                     ARQQYAAAAFAGGGWGHGRGERRGAPTQSGPGVTASHHAAMQSGSPSSDEPIMARWAR
                     GYTRIDYLA"
     gene            complement(4501..4968)
                     /gene="fliJ"
                     /locus_tag="PLM74_06125"
     CDS             complement(4501..4968)
                     /gene="fliJ"
                     /locus_tag="PLM74_06125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HON42692.1"
                     /translation="MDSFNFRLQPVLEHRRRLENHAKQELARAKIAEHQAREVLTSME
                     VALGDGQSDMADARRAEIDASEVAVYQRYLDRLKQDIAEQGDIVTTLHVHSEQRRSEV
                     IDGMKARKVVEKLKERQYREYQIEVNRHEQKQVDEFATTRYNRSKGQTGAEHA"
     gene            complement(4985..6331)
                     /gene="fliI"
                     /locus_tag="PLM74_06130"
     CDS             complement(4985..6331)
                     /gene="fliI"
                     /locus_tag="PLM74_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HON42693.1"
                     /translation="MSADGVGPGGAGLAVDLSKYHNRLERLDIVRQSGRVAQVIGLVI
                     ESNGPQAQVGEVCYVHSRNSRAPVTAEVVGFREGKLLLMPLGDMAGIEPGSKIVAGGK
                     SLGVRVGDELLGRVLDGLGSPMDGKGQIHAVAEYPVSSPPPSPLARPRIAQPLTLGVR
                     AIDGLLTCGSGQRVGIFSGSGVGKSTLLGMIARNTRADVNVIGLVGERGREVLEFIEG
                     DLGAEGLARSVVVVATSDTPALVRIKGAMVATAIAEYFRDRGLNVMLMMDSVTRFAMA
                     QREVGLAIGEPPATKGYTPSVFALLPRLLERSGTSERGTITGLYTVLVDGDDLTEPIA
                     DAVRSILDGHIVLSRQLADRNHYPAIDVLKSVSRVMDNIVSPEHKLAAGRFRDVLATY
                     RESEDLINIGAYVSGSNSRIDQAVEMIDSLNEYLRQTKEERASYEEAVDRLLALAG"
     gene            complement(6321..>7080)
                     /locus_tag="PLM74_06135"
     CDS             complement(6321..>7080)
                     /locus_tag="PLM74_06135"
                     /inference="COORDINATES: protein motif:HMM:NF018358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HON42694.1"
                     /translation="PSGRRGTRWLSSVYKGTDVIRPAAQEASFVLRTDRPELEALRCL
                     GDGPEQEAGLTPEERAAHIIAAARREADRILADARAEGQMIRRRAREEGHEEGRQAGM
                     EQAMQEYEDGLGILTRTADVVVSQADALVRAAERDLARLAVAVAGKVIQRSVEQDPEI
                     VLGIARAAISQTEGTRSLVLRVSPKDMELVLSAHDELVKSSPDLRNLKVVEDPRIERG
                     GCVVEMEVGHVDARIDEQLNEIERAFMEEAEYVG"
BASE COUNT         1149 a         2452 c         1921 g         1558 t
ORIGIN      
        1 ttactgcccc attcgcatcg aacagcacca ctcctcgccc cactgcagac ggcggcgtgc
       61 ctgatgtcgc ttcccactcc cattcgttca tggtggccgt cttcgtaaac tccattgtca
      121 ccgtgtgctg aagtccctgg gagtcgtaca ccggtacgga tgtagcgtat ttgtcgcctg
      181 cggctgaacc ggattgtaga ttgccgacaa acgacacgtt ctgagtgggc tgagcgctca
      241 tgctggttcc cagtggtatc gctatggcct caatgggcag gctgcgatct atcttgcctc
      301 ccgaatcggc cttccagccc tgtatctggt aacccgtgga tgggtgcacc aggttgccct
      361 tagcatcgat cttgaatgcg ccgtctcttg taaagagcag gccgccggcg gaatcgcgaa
      421 ccacaaagaa gccgttgcct tgtatcgcca tgtcagtgag cttcccggtc gactcaaggt
      481 ttccgggcgt aaacacgata tctattgacc tgagcatggt ccccaggccg acctgcatgg
      541 gatcgactcc gccgcgattg tcggtggggg ccatggatcc cctgagtgtc tgcgaaagca
      601 tatcctggaa agtggccctg ccgtatttgt acccaacggt attaacgttg gctatgttgt
      661 tgccgatgac gtccatgcgc acctggtggg cgcgcgtgcc ggacaccccg gcgaacattg
      721 aacgcatcat ctttatcgac ctccgtctga gtattccggg tctcgcgggc gcccctgtcg
      781 cgggcgtccg agggcttcct acgagaggtc cagccctgtc acacgattac cgccgagtcg
      841 atgttggtga agacgttctc cttcatgttc tcaccatcga ccgcagtgat gacggtccgg
      901 tttctgatgc tgaccacaaa ggccttatcg tccacgagca ccagcgattc gcgagccccc
      961 ttggccgccg ccttgttcac tccaactgcc agtcgcgaca gctcttcgtc ccccaagttc
     1021 atccgccgcg ctgtgatcct ggcctgcgcg tgcgcggaga atctcagtaa cgcgggtgac
     1081 gccagctctc cggcgagcat ctcttcaaac gttgtgcccg gcgcactgcc tgctgctccc
     1141 agcgacgggg ctgttccggg ttgtaccggc cttgccggac ggatgggcct ggagtctaca
     1201 ggcacgctgt tcagtcgatc tatcttgtcg gtcgtcatct cgccacaccc ctacgagatc
     1261 ttcgtcacgt ggttcatgct caccctgacg tcgcccacct tcagaaacgg aagcccggct
     1321 attacctcca cggcgctcac acgcccagtt atcggtgttg tgtacccggg cggccaagcc
     1381 tcgatggtct tgcccaccaa ccctccggcc tcagctatca tctgcctaga catcatctca
     1441 gtcactgttg cgctcagatt ctggagctcc tcaaccatgg tgaattgcgc catctgcgct
     1501 atgaactcct gattcgacgc gccttcacct atatcgagat tccgtagttg cgtcaccaag
     1561 agctgcagaa actcgtcctt gcccaggctg cgtctgggcg cctccgacac tgccgcgtcg
     1621 tacgtagcca ctccagcgac tctggatgta tacactcgca tctccctcct ctctcaggcc
     1681 gtcaggccag gtagtcgatt ctggtgtagc ctcgggccca tcgggccatg atgggttcgt
     1741 ccgagctcgg gctgcctgat tgcattgctg cgtgatgcga agccgttacg cctgggccgc
     1801 tctgtgtggg cgccccgcgc cgttctcctc tgccgtggcc ccagccaccg ccggcgaagg
     1861 ccgccgcagc gtactgttgc cgagcattgg caccgttcca gctggatccg gaccagtccg
     1921 actcaaagtg cgccggcaca gctgcgctcc atccctcctg agctctagac ctagacgaca
     1981 ccgataaccc tgccaggtcc actccgtgct ccgccaggct gaccttgaga tcggagctgt
     2041 gcgactcgat gaaccgggcg atttcggcgt cgtcggccct gatgtcggcg gtacagatgc
     2101 ctcgaaccac cctgaccctt acctccacgc tgcccagatg ctcagggaca agcctggccc
     2161 taaacacccc tccgccttgc gctgctctcg cgctcacgtg ggacgctatc tgacgcatca
     2221 ccgacgatcc cgcctgctcg gtgaggaccg gtctgcccgc ctgggacgtc tgagacacgt
     2281 cgtggccttc cacaggcgct gccgcaacta ccggatcgcc cgcgactgcg agctccgggc
     2341 gggcgactgg cgccggttca cggtgttctg cgtgatcgga tgttgacgac actgagtatt
     2401 tctgctcagg ctgagactgg gcctgtttca ccttctccga cccttcgtcc cacagtgggt
     2461 tgggtaggtt gcgatggcgg gctagtgact caactagagc tgatcgaacc agagcctcgg
     2521 gccatcctct cgatcccgtc acaaccctcg aagcggtcat agtgccttcc ttacgcgggg
     2581 caatcggccg caaggcggtc tgccgcggtt ccgccgtgtc acccatgtca gccttgccgt
     2641 ctgcgaggtc aaccatctcc ggcatgatgc cgcctggttc tatcaccggc gcagactcgc
     2701 caatcggcgc acggccttct gctcgcgcgg gtgtgtaacg ctccagctct gtggtcacca
     2761 gctcttctgt ctcgatcaca gcgggccttt cgcgaaccac ccatgtgttc ccgtctgcgt
     2821 ccgccgtacc gcgaatgtcc gtaagtactg tcgaacgccc ctctggtcgt atcatgcttc
     2881 gttcccgtac caccgctacg gtgtgttcct gaacctgtgg caccacgtcg ggttccgtaa
     2941 ccggcccatc catcggtctg gaagaaccat tgagcaccgg ttcatcacgc tcgaccggcg
     3001 cagcagtgtg aacgttcttt gattccaccc ggatggctgt gtggcccgca tcgcccttcg
     3061 gttgcgccat gctcgccgag gtagatccgc tgaccggcgc ttcgccagcc cgaggtccac
     3121 tcgtcgtgca atcaacctgg ccgaccgtcg ggtcattcac caggtccgcc accatgtggg
     3181 caccctctgg ctgcgacgcg cgtccgtgca ctggaaccgg cgtgtgatct cccggctgct
     3241 gtgacacgcg gtcctgtttg cctgcggatc gaccttctgc cagttgctcc ggatctgcgg
     3301 tcactggtcg tgtcgtgctt cgttcctgta tcaccgctac ggtgtgttcc tgcacctgtg
     3361 gcaccacgtc gggttccgta accgacccgt ctatcgatct ggaagaatca ttgagtaccg
     3421 gtgcatcacg ctcgaccggc gcagcagtgt gaacgttctt tggttccacc tgtgtgactg
     3481 tacggccagt gctgcctatc agtcgctcca tgctcattac cacatgttcg cgaaccggca
     3541 tttcggccgc tagagttgta ctctgcgtgc cgtcaacctg gccagcggtc aggtcattca
     3601 tatgatccac cgccatgtgg gcgccctccg gtgtgcgcct ctctggccct gctgtctctc
     3661 gtttctgtgc cgctgctgcg gtgcgttcct gcacctgtgg caccacatgc gattcggtca
     3721 ctggcccatt catcgatctc ggagaaccat tgggcatccg ttcatcgcgg ccgaccagct
     3781 cagaagcgcg aagcctgttt cgcgaaccat caacctgacc atccagtcga ccggctgatg
     3841 actgtaccgt actgcgactc cctgactgct gcgacatgcg gtcctgttcg tctacggatc
     3901 gaccttctgc aagccgctcc gcgctcaccg aagcgttctc ctgcatcgct atttcgtcca
     3961 tcagagatcc gctccgcgag tcctcaacct gcccggttgc ccgattatcc gtccgatccg
     4021 gcgtcgggtg gcgcggtgcg cgagtctgaa ccggcgcggt ccctgcagat ccagctgcca
     4081 tcatggccat ttgctgataa gcggcggtaa cagggccatc cacgtcttcg tcgccgtcgt
     4141 cgttgccgtc gtcatcctgg gcaggctgca tgtccaattc tgggtcggcg catgtgaccc
     4201 gggcgccatg cccgtttcgc ttcgaggcct ctgccgccac ggttggttct gccccgtatc
     4261 gcggctcggg tgtttgcagc tgccccgcca tctgctcaag caaggtgtcg aactcgacgt
     4321 cgggtcctgc gtcgcatatg gtgggagcgc ccgcggttag gttcgttgcc gacgtcatgg
     4381 ctgcggtcac agccgcgtga gctgccggcg cgcaggcaca cccctgaacg agcagtgccg
     4441 ttgttgcatc catcttcctt gttcacctcc tttcgcgtgg tctaggcggg cgaccccagc
     4501 ttacgcgtgt tcagccccgg tctgcccctt cgagcggttg tacctggtag tcgcaaactc
     4561 gtccacctgc ttctgctcgt ggcggttgac ctcgatctgg tactcccggt actgccgctc
     4621 cttcaacttc tccacaactt tgcgtgcctt catgccgtca atcacctccg agcgtctctg
     4681 ctcggaatga acatgaagtg tcgtaacgat gtcgccctgc tcggcgatgt cttgcttcag
     4741 gcggtccagg tatctctggt atacagccac ctccgatgca tcgatctcag cgcgtctggc
     4801 atctgccatg tcactctgac catcacctaa ggcaacctcc atgcttgtga gcacctcgcg
     4861 tgcctggtgc tcagctatct tcgccctggc aagctcctgc ttcgcgtggt tctcgaggcg
     4921 cctgcggtgt tctagcacag gttgaagcct gaagttgaag ctgtccatgt tgatcctgac
     4981 cctcctagcc cgccagcgcg agcaacctgt caactgcctc ctcgtacgag gcgcgctctt
     5041 ccttggtttg tcgcaagtac tcattgagcg agtcgatcat ctctaccgct tggtcgatcc
     5101 tggaattcga accgctgaca tatgccccta tattgataag atcctctgac tctcggtatg
     5161 tggccaagac gtcgcggaac ctgcctgcag ccagcttgtg ctctggcgac acgatattgt
     5221 ccatcacccg gctgacactc ttgagcacgt ctattgcggg atagtggttt ctgtcggcca
     5281 actgcctcga caagactata tgcccgtcca aaatgctcct caccgcgtcg gcgatgggct
     5341 cagtcaggtc gtccccgtcc accagcactg tgtacaagcc ggtgatagta ccccgctcgc
     5401 tggtgccgga gcgttcgagc agtctcggaa ggagcgcgaa taccgacggc gtgtatccct
     5461 tggtcgccgg aggttcgccg atggccaggc ccacttcacg ctgggccatg gcaaaccggg
     5521 tcactgagtc catcatgagc atcacattga gcccccggtc tcggaagtac tctgcaatcg
     5581 cagtagcgac catagccccc ttgatccgca ccagagcggg ggtatcgctc gtggccacca
     5641 ccaccactga cctcgccaac ccctccgcgc ccaagtcgcc ctcgatgaac tccagcacct
     5701 cgcgcccgcg ctctcccacg agcccgatga cgttaacatc tgcccgcgta ttgcgggcga
     5761 tcatccccag cagtgtactc ttgcctactc cgcttcccga gaagatgccc acacgctgcc
     5821 cggagccaca tgtaagcaat ccgtcgatgg cacgtacgcc cagtgtgaga ggttgcgcta
     5881 tccggggacg tgcaagagga ctcggaggcg ggctcgacac cgggtactca gccacagcgt
     5941 gtatctggcc cttaccgtcc atcgggctgc ccagcccatc taggacccgt cccaaaagct
     6001 catcgccgac ccgcacgccc aatgacttgc cgcccgccac gatcttgctg ccaggttcga
     6061 tgccggccat atcgcccagc ggcatgagca ggagtttgcc ttcgcgaaat cccacgactt
     6121 ctgcagtgac aggcgcacgc gagttgcgcg agtggacata gcacacctca cccacctgcg
     6181 cttgcggacc gttagactcg atgaccaatc cgatcacctg ggccacgcga ccgctctgcc
     6241 tcactatgtc gagccgctcc agccgattgt ggtacttgct caaatcgacc gcaagtccgg
     6301 ctccgcccgg gccgacgcca tcagccgaca tactccgctt cctccataaa cgctctctct
     6361 atttcattca gttgctcgtc gattctggca tcgacgtggc caacctccat ctccacgaca
     6421 catccgcccc gttcgatgcg tgggtcctcg accactttga gattacgcag atcaggcgag
     6481 gacttcacta gctcgtcgtg ggccgatagc accaactcca tgtctttggg cgacacgcgc
     6541 agaaccaaac tgcgcgtccc ctctgtctgg ctgatcgccg ccctggctat ccccagcacg
     6601 atctccggat cctgctccac cgacctctgg atcacctttc ccgccaccgc caccgcgagc
     6661 cgggcgagat cgcgctcggc agcacgcacc aaggcatcag cctgcgacac aacgacgtcg
     6721 gcggtgcgcg tcagaatgcc cagcccatct tcatactcct gcatggcctg ctccatcccg
     6781 gcctgccggc cttcctcgtg cccttcctct cgagcccgcc ggcgtatcat ttgcccctcc
     6841 gcccgggcgt ccgcgagaat gcggtcggcc tcgcgccttg cggcggctat gatgtgtgcc
     6901 gccctctcct caggcgtcaa ccccgcctcc tgctccggcc cgtccccgag gcagcgaagc
     6961 gcctccagtt ccggccggtc tgtgcggagc acgaaggatg cctcctgcgc cgccggacgg
     7021 ataacatcag tgcccttgta gacgctagac aaccatctcg tcccccttct cccggatggc
//
LOCUS       DAOTAC010000114         6983 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_9161_length_6983_cov_4.475173, whole genome shotgun sequence.
ACCESSION   DAOTAC010000114 DAOTAC010000000
VERSION     DAOTAC010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6983
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9161_length_6983_cov_4.475173"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="PLM74_06140"
     CDS             <1..480
                     /locus_tag="PLM74_06140"
                     /inference="COORDINATES: protein motif:HMM:NF015113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HON42695.1"
                     /translation="YRQVLDVLEHRASREEMFDRTLFATRQLAKRQTTWLRSFPGVGV
                     ACGMERCVLAAQANAAGQEHQVGLDVFIVSLGDAARERAFELAYDMRRAGLAVDFDMI
                     GRGLKAQMRYADKMKARFVAIIGDDELAAGEATVKDMSTGEQIRVAQAGLVTCMRRQ"
     gene            651..829
                     /gene="ssrS"
                     /locus_tag="PLM74_06145"
     ncRNA           651..829
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PLM74_06145"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            936..2273
                     /locus_tag="PLM74_06150"
     CDS             936..2273
                     /locus_tag="PLM74_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HON42696.1"
                     /translation="MTNSDLFDFGAEQRDAACGGMSREPLAARMRPRTLDDIVGQGHI
                     LGPGRLLRRAIEADRLGSIILYGPPGSGKTTIAEVVAATTQAVFERLNAVTSGVADIR
                     KVVSEARDRWKLHGQRTILFIDEIHRFNKAQQDALLPHVESGTVTLVGATTENPFFDV
                     NPPLVSRSRIFKLEPLTTDDLRGLVIRALTDAQRGLGAMPVEVAPEALDHIANFANGD
                     ARTALNAIEVAALSTPPGPDGKIHVTLAVAEDSIQRRALKYDAGGDMHYDVVSAFIKS
                     MRGSDPDAALHYLARMIESGEDPRFIARRLMIHASEDVGMADSHALLVATSALAAVEK
                     VGLPEAGIILAHATIYIAAAPKSNSVVKAISAALDDVRQMPVSDVPPHLRDASYRGAA
                     QLGHGAGYKYPHDYPGSWVKQQYMPDELRGREYYTPGSNPVEERVRQRLHSLR"
     gene            complement(2470..2799)
                     /locus_tag="PLM74_06155"
     CDS             complement(2470..2799)
                     /locus_tag="PLM74_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HON42697.1"
                     /translation="MTGPQVAPMVAIAIILAVTGMASFIFGHLIGDKRRFTLINGYTD
                     EKMLEPDRFARWMRVCYYIMGLVLLASAALIVLTRAVVAVVLVSTIIVAGILIVMTVG
                     ARRYYRI"
     gene            complement(2796..3380)
                     /locus_tag="PLM74_06160"
     CDS             complement(2796..3380)
                     /locus_tag="PLM74_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007781541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HON42698.1"
                     /translation="MSETTNVMLVGVGGQGTILASRVLAQLLLDAGFDVKMSEVHGMA
                     QRGGSVTTHVRFGRRVAAPTISPGQAHFVLAFECMEAARYAHYLKVDGVIVANTRRIP
                     SLPILIGHEAYPDDIENRLATRGIDAHWLDASGIAADLGNVRVENTVLLGAFSSLVAF
                     PEDAWAQALRTVIPAKHIELNLAAFAAGRRLTQQ"
     gene            complement(3380..5188)
                     /gene="iorA"
                     /locus_tag="PLM74_06165"
     CDS             complement(3380..5188)
                     /gene="iorA"
                     /locus_tag="PLM74_06165"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HON42699.1"
                     /translation="MKALMSGNEAIARGAYEAGARFASAYPGTPSTEILENMARYQEI
                     RSQWSVNEKTALEAALGASLGGARALCAMKHVGLNVAADPLFSSSYTGVNAGLVIVTA
                     DDPGCHSSQNEQDNRYYARAAKLPLIEPSDSQEALDFTRMAFELSEEYDTPVLLRVTT
                     RIAHGKSPVATGSRTEATAPAYERTMAKHVLVPAVARPRHAAVEERLARLQAYAESSP
                     MNRIEWGSRELGIISSGVAYQYAREVLGDNASYLKLGMSYPFADALVRSFISQVEQVI
                     VIEELEPFLEQAVRALGMGSERSIDGKSLIPRTGELDALTVALSLAPVLSAAKRGPAD
                     LSAPGRQVEQVVGRPPMFCAGCPHRGVFYELGKLPVMVSGDIGCYALGAMPPFSAMDT
                     CIDMGASITAGLGFQTALDILRPDKPTRTIAVIGDSTFFHSGMTGLLDAAYNDVGVIT
                     IILDNRITAMTGHQENPGSGFTLSGAPAREAQLEAMVRAIGIEMIQTVDPFDLAQVRE
                     AIASAMSNPGPSVIIARGPCALLKRLAVRRPAYHVDQDICRRCKACLRVACPALYVEG
                     GNVQIDAETCVGCGICSQVCRFGSIRQAGVAKDGDE"
     gene            5626..6057
                     /locus_tag="PLM74_06170"
     CDS             5626..6057
                     /locus_tag="PLM74_06170"
                     /inference="COORDINATES: protein motif:HMM:NF013482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42700.1"
                     /translation="MTDPAGYGLGRSIERIEGLEKVMGTAKFILDTCEPGMLYARMVT
                     SPHAHARIVSICASEAEMMPGVQAVLTGASSSIMCGTLLADRPVIAADKVRYHGEPVA
                     VVVAASELEAMRAAEKIQVEYEPLPVVNSPTEGMKPGRIAR"
     gene            6069..6500
                     /locus_tag="PLM74_06175"
     CDS             6069..6500
                     /locus_tag="PLM74_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42701.1"
                     /translation="MSQIPQSDHVALEPRAAPGGTRPELGELLVRVDTDMSYTSVPHA
                     FVKKTRTSKVDHPLPSIAAIRVDGRVRAACSGVGAAPFRSVEIEGALNSPELSLASRI
                     DQALERLPSPIIDDILGSGAYREFVLRSVLEDIIAELGGVS"
     gene            6512..>6983
                     /locus_tag="PLM74_06180"
     CDS             6512..>6983
                     /locus_tag="PLM74_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016114443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HON42702.1"
                     /translation="MLQTGMISLRVNGIASDVIVRPADTLLYALRERLRLTSAKPACK
                     TGDCGACTVLVYGITVLSCMMLAVEAIGHEITNIEGLRIAPFQREFRAALAESCGYCT
                     PGFVVNCHALVTLHPDASDEMIDEWLESNLCRCTGYEEIRAAVQSALVRAASGRV"
BASE COUNT         1367 a         2000 c         2256 g         1360 t
ORIGIN      
        1 taccggcagg tgcttgacgt gcttgaacat cgcgcgtcgc gggaggagat gttcgaccgc
       61 acgctgttcg ccacgcggca actggcgaag cggcagacga cctggctgcg cagcttccct
      121 ggtgtggggg ttgcctgcgg aatggagagg tgcgtacttg cggcgcaggc gaacgccgcg
      181 ggtcaagagc accaggtggg tctggacgtg ttcatagtat cgctgggtga cgcagcgcgc
      241 gagagagcct ttgagctggc gtatgacatg cgtcgcgcag ggctcgcagt ggacttcgac
      301 atgataggaa ggggcctgaa ggcgcagatg cgctatgcgg acaagatgaa ggcgcgcttc
      361 gtggccataa tcggagacga cgagctcgca gccggagagg caacggtcaa agacatgtcc
      421 accggggagc agatacgcgt tgcccaagcg ggactggtga cctgcatgcg ccggcagtag
      481 tcggtccggc cgggatctgg gcggtttgtg ggcacagtgg ctgggtgcaa tgacaaccaa
      541 tgatggaagt acctgcaata catgcctatt ggtcacccag tttggtaagt gtggctttgt
      601 tgaaacgcac tcgggcgtgt gatatcatat agccgacatc tgattgagct gttaccctac
      661 tgtgcgcgta ggcgcgctgg ctgtttgctt ccaacaaagc aaactcggga gcctggtctc
      721 tatcaccggc atgtgggccg ctttatgcgg aacgttaacg tggtaggagg gcacccacct
      781 cgcaggggcg ggggagaaac ggccagccct acggcatggt ggggtccttt tgtgtacgcg
      841 atggtgtcgg ccctgaagga tcggcacagc ccagtggcga aaccgggacc acgggagatg
      901 ccggaggata cgcggagtac gaggtgacca tatgcatgac gaattccgat ctttttgact
      961 tcggcgctga gcagagagat gcagcttgcg gcgggatgag ccgggagcca ctcgcggcgc
     1021 gaatgaggcc aaggacgctc gacgacatag tggggcaggg ccatattctc gggccgggca
     1081 ggcttctgcg acgggctatc gaggccgacc ggctgggatc cataatcctc tacgggcctc
     1141 cggggagcgg caaaactacc attgccgaag tggtcgccgc cacgacgcag gcggtgtttg
     1201 agcgactgaa cgcggtgacg agcggagtgg ctgacatcag gaaggtggtt tccgaggccc
     1261 gcgacaggtg gaaactgcat ggtcagcgta ccattctttt catcgacgag attcacaggt
     1321 tcaataaggc gcagcaggat gctctgctcc ctcacgtgga gagcggaacc gtgacgctgg
     1381 tcggggcgac tacagaaaac cccttcttcg atgtgaaccc tcccctggta tcgcggtcgc
     1441 gcatattcaa gctggagccc ttgaccaccg acgatcttcg cgggctggtg atacgcgcgc
     1501 tcaccgatgc gcagcggggt ctgggcgcta tgccggtgga ggtcgcgccg gaggctctcg
     1561 accacatcgc caactttgcc aacggcgacg cgcgcaccgc cctgaatgct atcgaggtag
     1621 cggcgctatc cacgccgccg gggccggacg gcaagataca cgtcacgctg gctgtggccg
     1681 aggactccat ccagcgcagg gctctcaagt acgatgccgg cggggacatg cactatgacg
     1741 tggtatcggc cttcatcaag agcatgcgcg ggtcagatcc tgacgctgcc ctgcattacc
     1801 tggcacggat gatcgagtcg ggcgaagacc cgcggttcat cgcccgccgc ctgatgatcc
     1861 acgccagcga ggatgtggga atggccgatt cgcatgctct gttggtcgcg acatcggctc
     1921 ttgcggcagt cgagaaggtg ggtctgcccg aggcagggat cattctcgcc cacgcgacta
     1981 tctacatcgc cgcggctccc aaaagcaact cagtggtcaa agccataagc gctgccctgg
     2041 acgacgtgag acagatgccg gtgtcggacg ttcccccgca cctgcgggat gcgtcatacc
     2101 gcggcgccgc gcaacttggg cacggcgcgg gttacaagta tccccacgac tacccgggct
     2161 cctgggtcaa gcagcagtac atgccggatg agcttcgggg acgggagtac tacacacccg
     2221 gatcgaaccc ggttgaggag cgggtgcggc agaggttgca ttcacttagg tgaatctgtg
     2281 tgttgcccgc attcatggac cttcctgggt tcataggcgt ggataggctg cgcacccctg
     2341 gtgcctagga gtggggttgg gcagcatatg ccaacgtttg cccacgattt gcagatgagc
     2401 tgcagagagc ttgcgcatcc tgttcgtttt tgggccagga actccaagag cgtccgacat
     2461 gagtgagcgc tagattcgat agtatcgtcg tgcccccact gtcatcacga tgaggattcc
     2521 tgccactatg atagtggaca caagcaccac ggcgaccaca gcacgcgtga gcacgataag
     2581 ggccgcagaa gccaggagca ctaggcccat aatgtagtag cagactcgca tccatctggc
     2641 gaacctgtcg ggctccagca tcttctcgtc ggtgtagccg ttaatgagag tgaaccttcg
     2701 cttgtcgccg atgaggtgcc cgaagatgaa cgacgccatg cccgttacgg ccaggatgat
     2761 cgctatggcg accatgggcg cgacctgtgg ccctgtcatt gctgcgtcaa cctcctgcct
     2821 gcggcgaacg ccgccaggtt cagttcgatg tgctttgccg ggatcaccgt gcggagcgcc
     2881 tgagcccagg cgtcttccgg gaacgcgaca agtgaggaga aggctccgag cagcactgtg
     2941 ttttccacgc gcacgttgcc gagatctgcc gcgattcctg aggcgtccag ccaatgcgcg
     3001 tcgatgccac gggtggcgag gcggttctcg atgtcgtccg gatacgcttc gtggcctatc
     3061 aggatgggca gcgaaggtat tcgcctggtg ttggccacaa tcacgccgtc tactttgaga
     3121 tagtgcgcgt atctggcggc ttccatgcac tcaaaggcca gcacaaaatg ggcctgcccg
     3181 ggtgaaatcg tcggggcggc tacgcgccgg ccgaaccgca cgtgtgtcgt tacactgccc
     3241 ccgcgttgcg ccattccatg cacttccgac atcttgacgt cgaatcctgc atccagcagc
     3301 agctgcgcca gtacccggct ggccaggatt gtgccttgcc cgcctacccc gacgagcatt
     3361 acgttggtcg tttccgacat cactcgtcac catcctttgc cacaccggcc tgtctgatgg
     3421 aaccgaaccg acacacctgg ctgcagatgc cgcatcccac gcaagtctct gcatcgatct
     3481 gcacgtttcc gccctctacg tagagcgccg gacacgccac gcgcaggcat gctttgcacc
     3541 tacggcatat gtcctgatct acgtgatagg cggggcgcct cactgccaac cgcttgagca
     3601 gcgcgcacgg gccgcgggca atgatcacgg aagggcccgg gttgctcatg gccgatgcga
     3661 tggcctcgcg tacctgggcc aggtcgaacg ggtccacggt ctgaatcatc tcgataccga
     3721 tggcgcggac catggcctcc agctgtgctt cgcgcgcggg agctccggag agagtgaagc
     3781 ctgaccccgg gttctcctga tgcccggtca tagctgtgat gcggttgtcg aggatgatgg
     3841 tgatcacgcc tacgtcattg taggccgcgt ctagcagtcc cgtcattccg gagtgaaaga
     3901 acgtcgagtc gccgatgacg gctatcgtgc gggtgggctt gtcgggcctg agtatgtcca
     3961 gagccgtctg gaatccgagc ccggcggtga tcgacgcgcc catgtctatg caggtatcca
     4021 tcgctgaaaa tggtggcatg gcgcccagag catagcagcc aatgtctcct gaaaccatca
     4081 ccggcaactt gcctagctcg tagaagactc ccctgtgggg acaccctgcg cagaacatgg
     4141 gcggtcttcc tactacctgc tcaacctggc gacctggggc agagaggtcg gccggcccgc
     4201 gcttggcggc ggacagtacc ggcgcaaggc tcagtgccac agtgagagca tctagttcac
     4261 cggtgcgggg gatgagcgat ttgccatcta tgctgcgttc agagcccatg cctagagcgc
     4321 gtactgcctg ttcgaggaag ggctccagct cttcgatgac tatcacctgt tccacctggg
     4381 agatgaagct tcgcacgagg gcgtcggcga atggatagct catgcccagc ttgaggtagg
     4441 aggcgttatc gcccagcact tcacgggcat actggtaggc tacgccgctg gatatgatgc
     4501 cgagttcgcg tgatccccat tcgattcgat tcatgggaga cgattccgcg taggcctgca
     4561 accgggccag gcgttcctcc accgctgcgt ggcgcgggcg cgctactgcc gggacgagta
     4621 cgtgtttggc catcgtgcgc tcgtaggccg gcgcggtggc ctcagtgcgc gagccggttg
     4681 ccacgggact tttgccgtgg gctatcctag tcgtcacgcg gagcagcaca ggtgtatcgt
     4741 attcctcgct cagctcaaag gccattcttg tgaagtcgag cgcctcctgg ctgtcggacg
     4801 gttcgatcag cggaagtttg gcagctcgag cgtagtaacg gttgtcctgc tcattctgtg
     4861 agctgtggca ccccggatcg tccgcagtca cgatcacaag cccagcgttg actccagtgt
     4921 atgaggacga gaagagggga tccgcggcta cgttcaaacc tacatgcttc atggcgcaca
     4981 gcgcccgcgc gccgccaagg ctcgcgccga gcgcggcctc aagagcggtc ttctcattta
     5041 ccgaccactg cgaccggatc tcttgatacc ttgccatgtt ctccagtatc tcagtgctcg
     5101 gagtgcccgg gtatgccgag gcgaacctgg caccggcctc ataggccccg cgggctatcg
     5161 cttcgtttcc cgacatcaag gctttcaagt acatccctcc cgcgtgatag aagcggcgaa
     5221 acacaggacg agtagcagga accgatgagg ttttcaacac atgatgccga agatccttca
     5281 cggcgttgtt gcccgtatgc gccgcacgca gacgggtttg ggcaaaaatc cgtatgtaga
     5341 tctatgcatc acccacatgg ctagaaacga cgcgccattg ccggcgcgga tgtagattgg
     5401 ggtttagtgg gccggagggg agggctgccg caacggatag gccgaaccac atccgctgca
     5461 caatcacgaa tgctgtaaga ctttcgtgcg caaggatgca tacgaggcca cgctggacgg
     5521 agtccataga cgacagactt agggcgctgg gcgcagcggc gcaatagcag ggcaggcagt
     5581 ttggggttca acagaggttt ccgaagcggg aggtcgcatg gccgcatgac cgatccggct
     5641 ggttacgggt tgggtagaag catcgagcgc atagaaggcc tcgaaaaggt tatgggaaca
     5701 gctaagttca tactcgacac ctgcgagccg ggtatgctct acgcccgaat ggttacaagt
     5761 ccacacgcgc atgcgcgaat agtctcgata tgtgcgtctg aggccgagat gatgcctggg
     5821 gtacaggctg tgctgaccgg ggcaagctct tccataatgt gcggcaccct attggccgac
     5881 cgccccgtga tcgctgccga caaggtcagg tatcacggag aacccgtggc tgtagtggta
     5941 gccgcgagcg agcttgaggc catgcgtgcg gcggagaaga ttcaggttga gtatgaacct
     6001 ttgccggtag tgaactcgcc gactgaaggt atgaagcctg ggcggatagc gagataatcg
     6061 tcgaatccat gtcgcagatt ccgcagtccg atcacgtcgc tctggaaccc cgggctgcgc
     6121 ctggcggcac gagaccggag ctgggcgagt tactggtccg tgtcgacacg gacatgtcgt
     6181 acacctccgt tccgcatgcc tttgtcaaga aaacgagaac ctcgaaggtc gaccatcccc
     6241 tgccgagtat cgcggcaatt agggtagacg ggcgggtcag ggcggcgtgc agcggggtcg
     6301 gcgcagcgcc cttcaggtca gttgagatcg agggcgccct caactcacct gaactcagtc
     6361 tggcatcgcg aatagaccag gccctggaac gcctcccatc tcccataatc gacgacatct
     6421 tgggatcggg ggcatacaga gagttcgtgc tgagaagcgt gcttgaagac atcatcgcgg
     6481 aactaggagg ggtgtcttag tggagcagac gatgctgcag actgggatga tatcgctccg
     6541 ggtgaacggg atagcgagcg atgtcatcgt aaggcccgcc gataccctgc tgtacgcact
     6601 gcgcgaaagg ctgaggctaa ccagcgccaa gccggcatgc aagaccggcg actgcggggc
     6661 atgtactgtt ctggtttacg gaattactgt actgtcatgc atgatgcttg cagtggaggc
     6721 cattgggcat gagatcacca acatcgaagg actgcgcata gccccttttc agcgggagtt
     6781 ccgagctgcg ctggctgagt cgtgcgggta ctgcactcct gggttcgttg tgaactgcca
     6841 tgcgcttgtg actctgcacc ccgacgcctc ggatgagatg atagatgagt ggctggagtc
     6901 gaacctgtgc aggtgcacgg ggtatgaaga gataagggct gccgttcagt cggcgctggt
     6961 ccgggctgcc tcagggcgcg tgt
//
LOCUS       DAOTAC010000115         6959 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_9199_length_6959_cov_3.762457, whole genome shotgun sequence.
ACCESSION   DAOTAC010000115 DAOTAC010000000
VERSION     DAOTAC010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6959
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9199_length_6959_cov_3.762457"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="PLM74_06185"
     CDS             <1..400
                     /locus_tag="PLM74_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HON42703.1"
                     /translation="PFLGGDTFEAPEFIEIGGKNVEGVYFSTHYSADAPVTPASEKFV
                     AQYKAKFKTAPNTFAALGYDCYNLILDAIERAGSSDPKAIRDAIAKTKGFVGATGIIT
                     LDSNGDATKSAVVLVVKDGQFKLVDVVHPR"
     gene            512..1405
                     /locus_tag="PLM74_06190"
     CDS             512..1405
                     /locus_tag="PLM74_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HON42704.1"
                     /translation="MTVEIFLQNVANGITLGSLYALLAIGYTMVYGILRLINFAHGEI
                     FMISTYFAFYGTAIFALPWQVSFIVAIVLTALLGVVIERAAYRPLRDAPRISALISAI
                     GVSFLMANLGLVIFGGRPKTFYRPPIFQAPIQLGGVRVVALFFVIPLLTAILLVALNY
                     IVYKTKTGLAMRAAQWDFDTARLMGVDVDRAISITFAIGSALAAVGGIMWGLRFAEIN
                     PNMGLFPGLKCFIAAVFGGIGNISGAVLGGFILGFGEIMLVAFLPGLAGYRDAFAFIV
                     LILILLVRPTGLLGDKQRVKV"
     gene            1402..2481
                     /locus_tag="PLM74_06195"
     CDS             1402..2481
                     /locus_tag="PLM74_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015393620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HON42705.1"
                     /translation="MMAERKVVHPALKIAAVCGALVLFFLVLSWGQEHWDAYTVRILN
                     LCGIYIGITLGLNLCNGFTGQFSLGIAGFMAVGAYTTALLTMSPAIKQAVFFIQPLIY
                     PLNSIQWGFLPALLMAAVVSGIVGFLVGAPAIRFRGDYLAITTLGFAEIIRVVFTNIV
                     PITNGALGLKGIPPHTNLLWSWGFALICIVVMVRLMRTTYGRALKAIRDDEVAAEAMG
                     VNLFFHKNLAFVMSAMMAGVGGGLLGNLITTIDPNMFRFPLTFTVLMMAVLGGLGSIT
                     GTVISAAAVTVAMEAFRVLEEPRVILGLSIPGVPGMRMVVFSIALMIVILFYPKGFMG
                     DREFSLDRVKRFAAGLSRANTSKQG"
     assembly_gap    2543..2642
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2643..3443
                     /locus_tag="PLM74_06200"
     CDS             2643..3443
                     /locus_tag="PLM74_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013681152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON42706.1"
                     /translation="MSDTNAKAAPALVCESVTMRFGGLTAVSGFDLRVPKGALYGLIG
                     PNGAGKTTVFNIVSGVLCPSAGAVRLFGTDITGLSPDRVAHSGIARTFQNIRLFRNLT
                     VLDNVLVGLHLRRQANVVEALLALPRHSAEEAQMAATATKLLEEIGLADVSHYRASAL
                     PYGQQRRLEIARALATKPAVLLLDEPAAGMNPAETEDLMRLITDIRDRFSLTILLIEH
                     EMKVVMGICERMTVLDHGETIAEGTPEEIQRNERVIKAYLGGGAVVRH"
     gene            3430..4146
                     /locus_tag="PLM74_06205"
     CDS             3430..4146
                     /locus_tag="PLM74_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009622422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON42707.1"
                     /translation="MLDIKDLQVYYDGIHAIKGVSLEVARGALVTLVGSNGAGKSTLL
                     RTICGLKKPSSGELRYQEQNLLDMSVTKIVQMGISMVPEGRQIFPDLTVMENLRLGAY
                     TRTDQDGIARDQERVFSLFPRLKERASQKGGTLSGGEQQMLAIARGLMAKPSLLLMDE
                     PSLGLAPILVEEVFRVIQEIHAEGVTVLLIEQNAQAALAIADYGYVMESGSIALQGKG
                     QDLLHDEAVQRAYLGQGVRV"
     gene            complement(4119..4577)
                     /locus_tag="PLM74_06210"
     CDS             complement(4119..4577)
                     /locus_tag="PLM74_06210"
                     /inference="COORDINATES: protein motif:HMM:NF024865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HON42708.1"
                     /translation="MWIAVAAIVLLATGINGCGGSKPTNPRGTPNGTLDELEKAIRRE
                     DIDAMLACYTDPFYVIVNSDVTPVTHDIARIAYAIAFAAYDYSTWSLVGRNVTISGSE
                     AFASCTQTMYFGGEWASAPVMYRMCRVGSMWYIYEESTVNLAYTLTPCPR"
     gene            4793..5326
                     /locus_tag="PLM74_06215"
     CDS             4793..5326
                     /locus_tag="PLM74_06215"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009461420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HON42709.1"
                     /translation="MQNNHLPLVTIKLDNGATIGAELYPEVAPNTVNSFISLIGKGFY
                     DGLTFHRVIPGFMIQGGCPKGIGIGGPGYTITGEFSGNGFRNDLKHTPGVLSMARSQH
                     PDSAGSQFFIMVGTSPHLDGSYAAFGKVVSGYEEVESIAQVARDRNDKPLAPQRMKKV
                     TVELFGVEYPEPTIMAD"
     gene            complement(5446..6579)
                     /locus_tag="PLM74_06220"
     CDS             complement(5446..6579)
                     /locus_tag="PLM74_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HON42710.1"
                     /translation="MRRLASIVLVVSLLVLGSLGTYAAVAAAEGTLLFSGKITAVDKY
                     GNSMTDISQGTVAASGAEVGDIVVVKIGENELVMPFVTIYGDVDRGTPLARISGGSVQ
                     LAINYGNFATKYGVSAGTEVSVYLREKGTYLSEIEIRHLTKLEKREDYSSDEAFANFR
                     QVTAGYIKPGVLYRTSHPSLGDDRSSYAARLVEKHGINTVLNLSDTPEESAANLAHSE
                     YYRDLNDKGNVIAVGMGVDFAAKDFTAKVKTALEFMIAHDGPYAVHCVEGKDRVGIVI
                     AVLEGLGGATVDEIVEDYMVSYENYYKVQKGTEGYKLVSKIMYDQLKEMNNGQPITDD
                     NVRAVVVNYLKNLVGLEDEQIEELETRLVGESSAQAEVASLAA"
     gene            complement(6716..>6959)
                     /locus_tag="PLM74_06225"
     CDS             complement(6716..>6959)
                     /locus_tag="PLM74_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42711.1"
                     /translation="DFTVTDHAQGVTFYWEHLGLLGDPEYRARWEMKRHEYMENGILS
                     WEDGGGEAGTLIETSDDAGGGLDAEKIAWLIDNVIR"
BASE COUNT         1437 a         2066 c         1874 g         1482 t
ORIGIN      
        1 gcccttcctc ggaggcgaca cgtttgaggc gcctgagttc atcgagatag gcgggaagaa
       61 cgtcgaaggc gtttacttca gcacccatta cagcgcagat gcacctgtaa ccccagcctc
      121 cgagaagttc gtggctcagt ataaggccaa gtttaagacc gccccgaaca cctttgcagc
      181 gctgggttac gactgctaca acctgattct cgatgccata gagcgcgcgg gttcgtccga
      241 tcccaaggca atccgtgacg cgatagccaa gaccaagggc tttgtgggcg cgacaggcat
      301 catcactctt gactccaacg gcgacgccac gaagagcgct gtcgtgcttg tggtcaagga
      361 tggccagttc aaactggtcg acgttgtaca cccgcgctag gtataggtta tcgctattga
      421 aagacgaggc aacggcagga taggacttcg actgctacgg gccggccctg caaaggatga
      481 ggcgtagtat ccgacaggag gcttgacgac ggtgactgtg gagatcttcc tgcagaacgt
      541 ggcaaacggg attaccctcg gcagtctcta cgcattactg gccataggat acaccatggt
      601 ctatggcatc ctgaggctca tcaacttcgc gcacggcgag atcttcatga tcagcaccta
      661 ctttgccttc tacggaaccg cgatctttgc actcccgtgg caggtttcgt ttatcgtggc
      721 aatcgtcctc acagccctgc tgggagtggt cattgagcgc gcagcttaca ggcctttgcg
      781 ggacgcgccc cgaatctcgg ccctgatctc cgccataggc gtctcgtttc tcatggctaa
      841 cctgggctta gtcatattcg gcgggaggcc gaagaccttc tatcggcctc ccatcttcca
      901 ggcccccatt caacttggag gagtcagagt agtggcgctc ttctttgtca tcccgctgct
      961 cacagccatc ctcctcgtcg cgctcaacta cattgtctac aagacaaaga ccggcctggc
     1021 aatgagggcc gctcagtggg atttcgacac ggcccgcctc atgggcgtgg acgtcgatcg
     1081 cgcgatctcc atcacttttg ccatcggctc ggctctggcg gcggtgggcg gaattatgtg
     1141 gggcctcagg tttgcggaga taaaccccaa catgggcctt ttcccgggcc taaaatgctt
     1201 catagccgcc gtgttcggag ggataggcaa catcagcggg gcagtcctgg gagggttcat
     1261 actcggcttc ggggagatca tgctcgtagc cttccttccc gggctcgccg gatacaggga
     1321 cgccttcgcc ttcatcgttc tgatcctgat tctcctggtc cggccaacag gcctacttgg
     1381 cgataagcaa agggtgaaag tatgatggcg gaacgaaaag tcgttcaccc ggcattgaag
     1441 atagcggccg tctgcggagc actggtcctc ttcttcctgg tcctttcgtg gggccaggag
     1501 cattgggacg cctacaccgt ccgtatcctg aacctttgcg ggatatacat cggcataacc
     1561 ctcggcctaa acctctgcaa cggattcacc ggccagttct ccttgggaat cgcaggtttc
     1621 atggcagtcg gggcctacac gacagcgctc ctcaccatgt ctccggcaat aaagcaggcg
     1681 gtcttcttca ttcagccttt gatatacccg ctcaactcga ttcagtgggg atttctgccc
     1741 gccctcctca tggcagccgt cgtttcaggg atcgtgggct tcctcgtagg cgcccctgcc
     1801 atacgcttcc gaggtgacta cctggcaatc actaccctgg ggtttgcaga gatcattcgc
     1861 gtggtgttca cgaacatcgt gcccatcacc aatggcgctc tgggcctcaa gggaatcccg
     1921 ccccacacga acctgctgtg gagctgggga tttgccctta tttgcatcgt cgtcatggtg
     1981 agactcatgc gcactaccta cgggcgagcc ctgaaagcca ttcgcgacga cgaggtggcc
     2041 gcggaggcca tgggggtcaa tctcttcttc cacaagaacc tggcgtttgt catgtcggcc
     2101 atgatggcag gcgtgggcgg agggctcctc ggaaacctca taaccaccat cgatccgaac
     2161 atgttcaggt tcccgctcac cttcaccgta ctgatgatgg cggttctcgg aggcctaggc
     2221 agcatcacgg gcacggtcat ctccgcagct gccgtgactg tcgcgatgga agcattccgc
     2281 gtgctcgaag agcccagggt cattctcgga ttgtccatcc ccggagtccc tgggatgaga
     2341 atggtagtct tctcaatcgc cctgatgata gtgattctct tctatcctaa agggttcatg
     2401 ggagatcggg agttttcgct tgaccgggtc aagaggttcg cggccgggct gagtcgcgca
     2461 aatacgagca aacaggggta gagaaagatg tccgacacaa acgccaaagc agcgcccgcg
     2521 ctggtctgtg aaagcgtcac aannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnatgtccga cacaaacgcc aaagcagcgc ccgcgctggt ctgtgaaagc gtcacaatga
     2701 ggttcggcgg gctcacagcg gtaagcggtt tcgacctacg cgtgccgaaa ggcgccctgt
     2761 acggattgat aggccccaac ggggccggca agaccacggt cttcaacatc gtgtcgggcg
     2821 tcctgtgccc gtctgccggg gccgtcaggc ttttcggcac ggacataaca gggctaagcc
     2881 cggaccgtgt cgcgcacagc ggcatcgccc ggaccttcca gaatattcgg ctcttccgca
     2941 accttacagt gctcgataac gtactcgtcg ggcttcatct gagacgccag gccaacgtcg
     3001 tagaagccct gcttgctctg ccccggcaca gtgcagagga agcgcagatg gcagcaacgg
     3061 caacgaagct acttgaggag atcgggctcg cagatgtgtc gcattaccgc gccagcgcgc
     3121 tcccgtacgg ccaacagcgc cgcctggaaa ttgcgcgggc gctggccacg aaacccgctg
     3181 tcctcctgct ggatgagccc gcagcaggga tgaacccggc ggaaacggag gacctcatgc
     3241 gcctcatcac cgacatccgt gacaggttca gtctcaccat actcctgata gagcacgaga
     3301 tgaaagtggt gatgggcatc tgcgagcgca tgaccgtcct cgaccacggc gagaccatcg
     3361 ccgaggggac gcccgaggag atccagcgca acgagagggt aatcaaagcc tatctggggg
     3421 gcggagcagt tgttagacat taaggatctc caagtatatt acgacggaat ccatgcaata
     3481 aagggagtct cgctcgaagt agcccggggt gcgctcgtca ccctggtggg ttccaacggc
     3541 gcaggcaaga gcaccctgct gaggacgatc tgcggcctca agaagccgtc gtcaggcgag
     3601 cttcgttacc aggagcagaa cctgctcgat atgagcgtaa ctaagatagt ccagatgggc
     3661 ataagcatgg tgcccgaagg aagacagatc ttccccgacc tcacagtcat ggagaacctc
     3721 cgcctcggcg cctacacccg aactgaccag gacgggatcg cccgcgacca ggagcgtgtg
     3781 ttcagcctat tcccaaggct caaggagcgc gcttctcaga aaggcggcac cctgtcaggc
     3841 ggcgaacagc agatgctcgc gatagccaga ggcctgatgg ccaagccgtc gctgcttcta
     3901 atggacgaac catcgctggg cctggccccg atcctcgtgg aagaggtgtt ccgcgtcatc
     3961 caggagatcc atgcagaggg cgtcaccgtg ctcctaatag agcagaacgc ccaggccgcc
     4021 ctggccatcg ccgactacgg ctacgtgatg gaatcaggaa gcatagccct gcagggaaag
     4081 ggacaggacc tgctgcacga cgaagcggtt cagcgcgcct accttgggca gggtgtgaga
     4141 gtgtaggcga gatttaccgt cgattcttcg tatatgtacc acatggatcc aacacggcac
     4201 atacggtaca tcaccggcgc agaagcccat tctccgccga aatacatcgt ctgcgtgcac
     4261 gaagcgaatg cctcagaacc actaatagtc acattcctgc caaccaacga ccacgtggag
     4321 tagtcgtagg ccgcgaacgc aatagcatat gcgatacgcg ctatgtcgtg ggtaaccgga
     4381 gtcacgtcac tgttgacaat gacatagaat gggtcggtgt agcaggcaag catcgcatcg
     4441 atatcttctc ttcgaatagc cttctcaagt tcgtccaggg ttccatttgg agtgcccctc
     4501 ggatttgtcg gcttgcttcc gccgcaaccg ttaatgccag tggccagaag tacaatcgca
     4561 gcgaccgcaa tccacattcc tatcctttat ttcctctgat tgatcatcgc attatcaacc
     4621 tccacccata tttgcccctg attccctcct atcttggtga ttatgcagag gtggccaaag
     4681 accttctttc tatgcttccc gccttccctg ggtctcaact gccatcatgt gctatcatgg
     4741 gtctatgcga gttgacgact gattcttcaa cagacctatt ggaggcgaat gcatgcagaa
     4801 caaccaccta ccgctcgtca ctatcaagct ggacaacggc gcgacaatag gagccgagct
     4861 ataccccgaa gttgccccga acacagtcaa cagcttcatc agcctcatcg gtaagggctt
     4921 ttatgatggg ctgacattcc accgcgtgat tccaggcttc atgatccagg gcggatgccc
     4981 gaaggggatc ggcatcggag ggcccggcta cacaatcacg ggtgagttca gcggcaacgg
     5041 attcaggaac gacctcaagc acacgccagg agtgctctca atggcacgct cgcagcatcc
     5101 cgactcagca ggctcgcagt tcttcatcat ggtaggcaca tcgccgcatc tcgacggcag
     5161 ttacgccgcc tttgggaagg tcgtgtcagg ttacgaagaa gtggagagca tcgctcaagt
     5221 agcgcgcgat cggaacgaca aaccgctcgc tccccagagg atgaagaagg ttacagtaga
     5281 gctattcggc gtggagtatc cagagcccac gataatggcc gactagcgct aggcgaacgg
     5341 tgctacaaag aagccgcctg gctcatcccg acatggaatg agtcaggcgg cccttattgt
     5401 gcgcaaccct cgagtctgcc tggcctggtc cgcaggcccc ggtcgttatg ccgccagcga
     5461 ggccacctct gcctgcgcag acgactcgcc cacgagcctg gtctcgagct cctcgatctg
     5521 ctcgtcttcc agtcccacca agttcttgag gtagttcact accacggccc tgacgttgtc
     5581 atcggtgata ggctgaccgt tgttcatctc cttgagctgg tcgtacatta tcttcgacac
     5641 cagtttgtag ccctcagtgc ccttctgcac cttgtagtag ttctcgtagg agaccatgta
     5701 gtcttcgaca atctcatcga ctgtcgctcc acctagaccc tcaagaactg ctatgactat
     5761 gcccacccta tctttgcctt ccacgcagtg aacggcatac ggcccgtcgt gcgctatcat
     5821 gaactccaga gcagtcttta ccttcgcagt gaagtccttc gccgcgaagt ccactcccat
     5881 cccgactgca atcacgttcc ctttgtcatt gagatcgcgg tagtactcgg aatgggcgag
     5941 gttagccgca gactcctcgg gagtatccga caagttcaaa actgtgttaa tcccgtgctt
     6001 ctcaaccagc ctggccgcgt acgacgaccg gtcgtcgcct agagacggat gtgatgttcg
     6061 gtataggacc cctggcttta tgtaccccgc tgtgacctgt ctgaagttcg cgaaagcttc
     6121 gtcgctgcta tagtcctccc gcttctccag tttcgtgaga tgacggatct ctatctccga
     6181 cagatacgtg cccttctctc ggagatacac actcacttca gtccctgcag acacgccgta
     6241 tttggtggcg aagttgccgt agtttatggc gagttgcaca ctgccgccgc tgattcttgc
     6301 gaggggggtt cctctgtcca catcgccgta gatcgtgaca aacggcatta caagctcgtt
     6361 ttcacctatc ttcacgacta caatgtcccc tacctcggcc cctgaagccg cgacagtccc
     6421 ctggcttatg tctgtcattg agttgccgta cttgtccacc gcagtgatct tgccgctgaa
     6481 gagaagggtg ccttctgcag ctgcgaccgc tgcataagta cctagagaac caagaaccag
     6541 aagcgagacg actaacacta tcgacgccaa tcttctcaag ttctacacct caaacctaac
     6601 tagtaccgtg gaatcccgca cttatcggtc gtcttcggcg gccgacatgg tgtccatggg
     6661 tcttgcatga tccgtggccg ttgcatcctt cctttccggg tccttgacga actgcctacc
     6721 tgatgacgtt atcaattagc catgcgatct tttcagcgtc cagtcctccg cctgcatcat
     6781 cgcttgtttc gatgagtgtt ccggcctcgc cgccgccgtc ttcccaggaa agaatcccgt
     6841 tttccatgta ctcgtgacgc ttcatctccc accgcgcccg gtattcggga tccccgagta
     6901 gaccgaggtg ctcccagtag aaggtcacgc cctgggcgtg gtctgtgacc gtgaagtcc
//
LOCUS       DAOTAC010000116         6947 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_9236_length_6947_cov_4.585607, whole genome shotgun sequence.
ACCESSION   DAOTAC010000116 DAOTAC010000000
VERSION     DAOTAC010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6947
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9236_length_6947_cov_4.585607"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            757..1386
                     /locus_tag="PLM74_06230"
     CDS             757..1386
                     /locus_tag="PLM74_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42712.1"
                     /translation="MPERPGRILAIGLWTLVLSAALSSSSGALAGTVNIGFSIAVLVA
                     IISAGILFDIIGTAATAASEAPINAMAANRVYGAPEALAIVRNAPAVANFCNDFVGDI
                     CGTVSGAVGATIAISAALAPGTLLSKFASTAVIALISATTVAGKAAGKEFAIESANSV
                     MLTVGRTVATFHRYFGGGRSTGPSRSGSVSRSNRGNGGSDRNGRNGGNA"
     gene            1389..3308
                     /gene="dxs"
                     /locus_tag="PLM74_06235"
     CDS             1389..3308
                     /gene="dxs"
                     /locus_tag="PLM74_06235"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HON42713.1"
                     /translation="MTSILQRIQCPADLRKLSDRQVGQLAAEIRQYMIDTVSRTGGHL
                     APNLGVVELTIAIHLALDCPRDKVIWDVGHQSYVHKLLTGRHGVFSSLRQQGGISGFP
                     RPEESPYDCFATGHSSTSISAALGMARARDLAGDDYTVLAVIGDGSFASGMAFEAMND
                     AGHANTPLIVILNDNEMSISRSVGGLASYLGSVRANPWRRQLRSRVQQAVLSIPVIGR
                     ALRALGHSIKRSVKSLLLRGMLFEELGFMYIGPIDGHNVRTVRRAIEDARQAGGPVLI
                     HVITRKGLGYAPAEANPARFHGTGPFDVRTGEAAPNERKSFTSHFSDFMVHAGAADDR
                     LVAITAAMADGTGLRAFAERFPDRFFDVGIAEQHAVTMAAGLAVGGRRPVVAIYSTFL
                     QRAYDQVLHDAAIENIPVVFAIDRAGIVGEDGYTHQGSFDVAFMRHIPKMTVMSPKDE
                     AELYSMMQAALDHMGPCAVRYPRSAAPGVGVRYPADPVEIGRAEVIRDGRHCAIFALG
                     PMVYPALEASRILSTRGVSCAVVNARFVKPLDTSILAQYASATGLIITCEDAALPGGF
                     GSAVLEALAQAGMGDVRVVRLGIPDRFIEHGRRDDILAQLGLDGRGVAAAVLRALSER
                     ADAKGSGGGIRVSTV"
     gene            3292..4119
                     /locus_tag="PLM74_06240"
     CDS             3292..4119
                     /locus_tag="PLM74_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HON42714.1"
                     /translation="MSQPCKSRLDQLLVERGLAPSREAGRREILAGCVWVAGMREDKP
                     GTMVRADSEIEIRRSGPRFASRGGEKLAHAMDVFELDAKGRTAVDIGASTGGFTDVLL
                     RAGAIRVYCVDVGYGQLAWELQQDPRVVVMDRTNARHLVPSMFDPRPDLATVDVSFIS
                     LSKILPALREVLTVQAEAVCLVKPQFEAGRDKVGKRGVVRSPEVHVEVLMNVAEYAWG
                     SGFCVAGVTASPIRGPEGNVEFLMYLFRGDHDRPSISVDALHGNVLRVVAEAHAQPR"
     gene            4170..4970
                     /locus_tag="PLM74_06245"
     CDS             4170..4970
                     /locus_tag="PLM74_06245"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HON42715.1"
                     /translation="MRKTKPEADLVALQIESWMKRAGIDTALDYSWDGPLPDSTDCVI
                     VLGGDGTLLSVARRAARAGIPILGVNMGNLGFLTEVEPVNLEEALEALVRGDYTIEER
                     TMVQATVMRTGKSVASLVGLNDVVISKGAFARIISLDIFVNDEYYSTFPADGIIISSP
                     TGSTAYSLSAGGPLISPSIDVMLVTPICPHTLFSRSLVIAGDDRVRVVVEAPHEEVAV
                     TVDGQVGFKVESHDEIIVERAPEVTRFVRLRAKSFYGTMRERLKQDRL"
     gene            4996..5448
                     /gene="argR"
                     /locus_tag="PLM74_06250"
     CDS             4996..5448
                     /gene="argR"
                     /locus_tag="PLM74_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HON42716.1"
                     /translation="MKARRHQRILDIVRSKPIQTQEDLSAELAREGMSVTQATISRDI
                     KELRLIKAPVGDGSYRYTVPVDRNIEDINRRIERVFREAVISVEDSDNIVVVKTLEGA
                     AQAIGAIVDDLDWPEIVGSLAGDDTIFVVVKPADKAPEIMARFNKFRR"
     gene            5468..>6947
                     /gene="recN"
                     /locus_tag="PLM74_06255"
     CDS             5468..>6947
                     /gene="recN"
                     /locus_tag="PLM74_06255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HON42717.1"
                     /translation="MLLEMDVRNYALIEHISIQFGDGFNVLTGETGAGKSLVIDCLGL
                     VVGGRASAESVRTGAESAVVEAVFDLSQMASMADILSEQGIDPEPDGTLVVSREVWPQ
                     GRSRCRVNGRTVTLTSLAAIGEFLVDIYGQHEYQSLARPSRHLALLDSFGGAELSKAL
                     AAYRAKYALWRSAHRELAQLKADARERARRSDLLAFEVNEIESAKLQPDEDVLLAAEH
                     TILANAERLLGGVSQAFELVYESSRPDSPSAYDMLADALAHLDAAARIDASLTPVRDL
                     LASMCEQAGSVGRAMRSYRESIDFSPQRLAAVEERLAAIARLKRKYGDTLAEVIEHGR
                     AAREALDLVVHADERSAELERRVAEYAEDAAGAAAEVSEIRGRIASRLEHDITCELSE
                     LSMPKAAFQVQIRKTRGDAEQELIVDGQPCILGPNGVDDVEFLFSANPGEEPRPLSRI
                     ASGGEMSRVMLAIKSVLAQVDDMPTMVFDEVDQGIGGEASMAV"
BASE COUNT         1367 a         1894 c         2276 g         1410 t
ORIGIN      
        1 agcactacgg aatagcttct tagctcttct ttcctactgt agattgacgc ggtcctgcag
       61 cgcgggtgat ttgaaccgcg cttgaaacgt atggtataat aaacgttgca gtggataggt
      121 ggcgcagtat cctagtcgga gcgggcactc ctgaaggcgg gggtaaaaga ccgctaaggg
      181 cacatcgatg aagtcccttg tgctggcttt caacgcccag ttgggggctg gtgcaggggg
      241 ttaaggttgt ggggcgatcc gcaatggcat gtgggcgtag accccgcttc cgcggaggcc
      301 caagtgcgtg gttggttcta cggcttgggg gtaacctgca tgcggtaccg gcctgcaagt
      361 gaatgcagaa tggtgttggg tgcagcgtag cctgccttga gtggacgtgc aggatgcggg
      421 tatcaagtca tgttctcccc ggccagggag aatgaccttc tgccgcctga aagcacgcct
      481 gcaaaagagg ctaaggaata gcccgcgctg ccgaggaaaa ctcctaggct gtttgcgatg
      541 cttggcgccc tggggattac agtgtgcact aagtggcaat ccagttgctc cttcggcgac
      601 gaggagagcc tgtcgtaaag ggaaaccgcc ccccggtgac gggagggtgt acaggcggga
      661 aaacctactg gaccttaagt cacaatattt actcggggcg ctgccaccta tcgacatgca
      721 tccacatcaa gcaagacggg atgaggtgaa catggcttgc cagaacgacc ggggaggatc
      781 ctagccatcg ggctgtggac tctcgtcctg tcggccgctt tgagttccag ctcaggggct
      841 ttggcgggta ccgtgaacat cgggttttcg attgcggttc tagttgcaat aatatctgca
      901 ggaattctgt tcgacataat tggcacagca gcgacagcgg ccagtgaggc gccgatcaac
      961 gccatggccg cgaaccgtgt gtacggggct cccgaagcct tagcgattgt acggaacgcg
     1021 cctgctgtgg ccaacttctg caacgacttc gtaggagaca tatgcggcac ggtaagtggc
     1081 gccgtcggcg ccacaattgc cataagcgct gccctggctc ccggtacgct tctatcaaag
     1141 tttgcatcca cggccgtgat agcactgatc tccgcaacaa ccgttgccgg caaggcggct
     1201 ggcaaggagt tcgccatcga atcagccaac agcgttatgc tgacggtggg gcgtacggta
     1261 gccacatttc accggtattt cggcggcggc agatcgaccg ggccctcccg atcaggcagt
     1321 gtatcgaggt caaatagggg aaatggcggg agcgatagga atgggagaaa tggcgggaac
     1381 gcatgaggat gactagtatt ttgcagcgga tccagtgtcc tgccgacctt cgcaagctat
     1441 ccgacaggca ggttgggcag ctcgcggcgg agatacgcca gtacatgatc gatacagtct
     1501 ctcgcaccgg aggccatctg gcgcctaacc ttggcgttgt cgagctcact atagccatcc
     1561 atctcgccct ggattgccca cgcgacaagg tcatatggga cgtagggcac cagtcgtacg
     1621 tgcataaact cctcaccggg cggcacggtg tcttttcttc tctgcgccag cagggaggga
     1681 taagtggttt cccaagaccc gaggagagcc cgtacgattg tttcgccacg gggcattcca
     1741 gcacttccat ctctgcagcg ctcggcatgg ctcgagcccg tgacctcgcc ggcgatgact
     1801 acacggtgtt ggctgtaatc ggagatggct cgtttgccag cggcatggct ttcgaggcca
     1861 tgaacgacgc cgggcatgcc aatactcctt tgatcgtcat tttgaacgac aatgagatgt
     1921 cgatatcccg aagcgtgggc ggcctcgcaa gctaccttgg atcagtcaga gccaatccct
     1981 ggcgcaggca gcttagatcc cgtgttcagc aggcggtact cagcataccc gtcatcggtc
     2041 gggcacttcg cgctctcggc cattcgatca agagaagcgt caagtctctc ctcttgcggg
     2101 gcatgctctt cgaggaactg ggattcatgt atatcggccc catcgacggc cacaatgtgc
     2161 gcacggttcg acgagcaatc gaagatgcgc ggcaggccgg tgggcctgtg ttgatccatg
     2221 tgatcacgcg aaagggccta ggctatgcgc ccgctgaagc caatccggcc aggtttcacg
     2281 gaaccggtcc gtttgacgta cggacaggcg aagcggcgcc caatgaacgc aagtcgttca
     2341 catcacattt cagcgatttc atggtgcatg ccggcgccgc tgacgataga ttagtcgcca
     2401 tcactgccgc catggccgac ggcactggtc tcagagcatt cgcagagcgc ttccccgatc
     2461 gtttcttcga tgtgggcata gcggagcagc atgcagtcac catggctgcg ggcctggctg
     2521 taggcgggcg ccgacctgtt gtagccatct actctacgtt tttgcagcgc gcctatgatc
     2581 aggtgcttca tgatgctgca atcgagaaca ttcccgtagt attcgccatt gaccgggcag
     2641 gaatagtagg agaggacggc tacacccatc aggggtcgtt cgatgtggct ttcatgcgcc
     2701 atatccccaa gatgacggtg atgagcccca aggacgaagc cgagctgtac tcgatgatgc
     2761 aggccgcttt ggaccatatg gggccgtgcg ctgttcgcta ccccaggtcg gctgcgccgg
     2821 gcgtaggtgt gcggtatccg gctgacccgg tggaaatagg gcgggcggaa gtgatccgcg
     2881 acggtcggca ttgcgccatt tttgctttgg ggccaatggt ataccctgct ctcgaagcga
     2941 gcaggattct gtcgacgcgt ggcgtaagtt gtgcagtggt gaatgccagg ttcgtcaagc
     3001 cgctagatac gagcatattg gcgcaatatg cgtctgctac gggcctgatc ataacgtgcg
     3061 aggatgcggc gcttcccgga ggtttcggtt cggcggtcct cgaagcgttg gcccaggcgg
     3121 gcatgggcga tgttcgcgtt gtcaggttgg gcattccgga tcgcttcata gagcatgggc
     3181 ggcgcgacga catacttgcc caacttgggc tggacggccg gggcgtggcc gctgcggtgc
     3241 tccgggccct ctccgagcga gccgatgcca aagggtcggg cggtggtata cgtgtctcaa
     3301 ccgtgtaagt cccgcctcga ccagctgctt gtagaacggg gcctggcgcc gagccgggag
     3361 gcgggtcgtc gtgagattct ggccggatgc gtctgggtcg ccgggatgcg tgaagacaaa
     3421 ccaggcacca tggtgcgcgc ggactcagag atagagatcc ggcggtctgg cccgcgcttc
     3481 gcgagtcgag gcggcgagaa actcgcgcac gccatggacg tattcgagct cgatgcgaag
     3541 ggacgcacag cggttgacat cggggcgtcc acagggggat tcacagacgt cttgctgcgc
     3601 gctggcgcga ttcgtgtgta ctgtgtggac gttggatacg ggcagctggc gtgggagctc
     3661 cagcaggacc cccgagtggt agtgatggac cgcaccaacg cgcgtcatct tgtgccctcg
     3721 atgttcgatc cgcggcctga ccttgcgacg gttgatgtct ccttcatctc gctttcaaag
     3781 atcctgcctg ccctgcgcga ggtgttgact gtgcaagccg aggctgtatg cctggtcaag
     3841 ccgcaattcg aggcaggtcg cgacaaagtg ggtaagcggg gcgttgtgcg ctcgccagag
     3901 gtccacgtcg aggtgttgat gaatgtggcg gagtacgcct ggggcagcgg gttttgcgtg
     3961 gcgggggtca cagcctcgcc catccgcggg ccggagggta atgtcgaatt cttgatgtat
     4021 ctgttcaggg gggatcatga ccgcccgagc atatcggtgg atgcgttgca cggcaatgtg
     4081 ctgcgagtag tcgccgaggc ccatgcacag cctagataag tccggctggt agcggaggag
     4141 gggttactac gaagaaggcc gcaatagtgg tgcgaaagac aaagccggag gccgacctgg
     4201 tagccctcca gatagagtcg tggatgaagc gcgcgggtat cgacactgct ctcgactaca
     4261 gctgggacgg gccgttgccc gattcgaccg attgcgtgat cgtgctgggt ggcgatggca
     4321 cgcttctttc agtggcgagg cgtgctgccc gggcggggat ccccatactc ggcgtcaaca
     4381 tgggtaacct gggcttcctt accgaggtcg agccggtcaa cctcgaagaa gctttggaag
     4441 ctctggtgcg cggggattac accatagaag agcgaacgat ggtgcaggcg actgtgatga
     4501 ggaccggcaa aagcgtcgcg tcgcttgtgg ggctaaacga cgttgtgatc agcaagggcg
     4561 cctttgcacg cattatcagt ctggacatct tcgtaaacga cgagtactac tccacattcc
     4621 cggctgacgg gatcatcatc tccagtccta cagggtccac tgcgtactcg ctctctgcgg
     4681 gagggccgct tatcagcccg agcatcgacg tcatgttagt aacccccata tgtccgcaca
     4741 ctctgttctc gcgctcgctg gtaattgcag gtgatgacag ggtgcgcgtg gtcgtggagg
     4801 ctccgcatga ggaagtcgca gtgacagtcg atgggcaggt tggcttcaaa gtagaatcgc
     4861 acgatgagat catcgtcgag cgggcgccgg aagttacgcg cttcgtgcgc cttcgagcca
     4921 agagcttcta cggtaccatg cgtgagcgtt tgaaacaaga ccgtctatga taccgacgtg
     4981 cgggaggggt cgatggtgaa ggccaggcgc caccagagaa ttctggacat agtgaggagc
     5041 aaacccattc agactcagga ggatctatcc gcggagcttg cacgcgaggg aatgtcggtt
     5101 acccaggcca cgatatcccg ggacatcaaa gaactgcggc tgatcaaagc gccagtgggc
     5161 gatgggtctt accggtacac cgttccggtc gaccgcaata ttgaagacat caacagacgg
     5221 atcgaacgcg tgtttcgcga ggccgtgata agcgttgaag acagcgacaa tattgtggtc
     5281 gtcaagacac tcgaaggcgc ggcgcaagcc atcggcgcca ttgtggacga tttggactgg
     5341 cccgagatag tgggctcact ggcaggcgat gatacgattt tcgtcgtagt gaaaccggcg
     5401 gataaagccc cggagataat ggccaggttc aacaagttcc gtaggtgaca gcggcggggg
     5461 cggagtcatg ctattggaga tggacgtccg aaactatgct ctgatagagc acatttccat
     5521 tcagtttggc gacggcttta atgtgctcac aggcgagacg ggcgcgggaa agtctctcgt
     5581 catcgactgt ctcggtctgg tcgtcggcgg ccgggcatca gcagaatccg ttcgaacggg
     5641 cgccgaatcc gccgtagtgg aggcggtctt cgatctgtca caaatggcct ccatggcgga
     5701 catcctttcg gaacagggaa tcgatccaga acccgacggc acgctggtgg tttcgcgcga
     5761 ggtgtggccg cagggccgtt cgcgatgccg agtgaacggc cgcactgtca cgctcacatc
     5821 gcttgcggcc atcggcgaat tcctggtcga catatacggc cagcatgaat accagtcgct
     5881 ggcccgtcct tcacgccact tagctcttct ggattccttc ggaggcgccg agctttccaa
     5941 ggccctcgcg gcttacaggg ccaaatacgc cctgtggagg tcggcccacc gtgagctggc
     6001 ccaacttaag gcagacgccc gtgagcgcgc tcgtcgatcc gatctcctgg cgttcgaggt
     6061 caacgaaatc gagtccgcga agctccagcc ggatgaggac gttcttcttg cggctgagca
     6121 cacgatactt gccaacgccg agcgcctttt gggcggggtg agccaggcat ttgagctggt
     6181 ctacgagtca agccggcccg attctccgtc ggcctacgac atgcttgccg atgccctcgc
     6241 ccatctggat gcggctgcgc ggatcgacgc ttccttgacc ccagtgcgcg atttgttggc
     6301 gtccatgtgc gaacaggccg gcagcgtcgg tcgggccatg cgttcgtacc gggaatccat
     6361 cgacttcagc ccccagcgcc tcgcagccgt ggaggagcgc ctggccgcca tagcgaggct
     6421 caaacgcaag tacggcgaca cgctggcaga ggtgatagag catgggcgcg cagcgcgcga
     6481 ggcgctggat ctggtggttc atgcagacga gcgctctgct gagctggaac ggcgggtggc
     6541 ggaatatgcc gaggatgcag ccggggcagc agcggaagta tcggaaatca ggggccgaat
     6601 cgcctctcgc ctggaacacg atatcacctg tgagctttcc gagctgtcga tgcccaaggc
     6661 ggctttccaa gtgcaaatcc ggaaaacccg gggcgatgcg gagcaggagc tcattgtgga
     6721 cggccagcca tgtattttgg ggccgaacgg tgtggacgat gtggaattcc tcttctcggc
     6781 caaccccggg gaagagccga ggccgctttc gcgcattgcc agcgggggcg agatgagccg
     6841 ggtgatgctg gcgatcaagt cggtgctggc gcaggtggat gatatgccga caatggtctt
     6901 tgatgaggtg gatcagggca tcgggggtga ggcgtccatg gcggttg
//
LOCUS       DAOTAC010000117         6871 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_9391_length_6871_cov_4.209507, whole genome shotgun sequence.
ACCESSION   DAOTAC010000117 DAOTAC010000000
VERSION     DAOTAC010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6871
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9391_length_6871_cov_4.209507"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /locus_tag="PLM74_06260"
     CDS             complement(<1..896)
                     /locus_tag="PLM74_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HON42718.1"
                     /translation="MKGRTVRHIAAVIAAILLASALAGCQRMSNTYSKHSDSLFDVFN
                     TLVTVVAYTRSEEEFNSHFAKIRTRFQKLHQLYDIYNDYQGVNNVKTINDNAGVQPVK
                     VDKELIDLLLFAKDWYRRTGGKTNVAMGAVLRIWHDYREEGQNDPEHAELPPMKDLRE
                     AERHTDIDKVIIDTENSTVYLSDSEMSLDVGAVAKGYATEIVAREMAEAGLKSALISS
                     GGNIRAIGKPFDGVRERWGVGIQNPDESIAPDEQNVLDVIFIKDSSVASSGDYMRYYI
                     VNGRVLHHIIDPATLMPADYYRA"
     gene            complement(1053..2078)
                     /locus_tag="PLM74_06265"
     CDS             complement(1053..2078)
                     /locus_tag="PLM74_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42719.1"
                     /translation="MKKAVSVLLLAAALFILVGWSPQQSAEKVGLGHVTSIASSKDLV
                     VDKNGKVTPPVAQVDTTMAAVAFDKAGRVIEVIIDTAQTKVNFDKDLKVTSDLTAENK
                     TKVELGDGYGMRKASSIKKEWFEQIAAFEKWMVGKTVAEIKSLKVKQRDASHPSVPDV
                     PELTSTVTMTVQDYIAAVEEAYKNAVDVPAGAVKLGLGHDISTAKSKAAAAEVNLTIA
                     ATMFDKDGKVVRTIIDTAQTKVPFDKDGKVAADKAGEFKTKIELGDGYGMRKASSIKK
                     EWFEQIAEFEKWMVGKTVAEIKSLKVKQRDASHPAVPDVPELTSTVTVTVGDYIAAVA
                     ESYANAK"
     gene            complement(2341..3303)
                     /gene="sdaAA"
                     /locus_tag="PLM74_06270"
     CDS             complement(2341..3303)
                     /gene="sdaAA"
                     /locus_tag="PLM74_06270"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HON42720.1"
                     /translation="MYTSIAELTDAATSRSLSIGATVREREALDSDQPEDALNSKMQE
                     RLAVMRDSVGRGLAGVRSRSGLTGGDARRMAEARTQTRSQTRTRTRTAIADGTAGGAP
                     VLIGGEPLASAIAYALAVAEVNASMGRIVAAPTAGSCGVLPGVLFSVGEIRGAGDDEL
                     IDALFAAGGVGAVIARSSTLAGAAGGCQAECGAAAAMAAAAAVQIAGGTPEQCANGVA
                     LAFKGLLGLVCDPVAGLVEVPCIKRNATSVAVALAAADMALAGIRSVIPADEVIWAMG
                     AIGRELPAALRETALGGLAATPTGKLIASEMNAVHDDAKESSRL"
     gene            complement(3333..4004)
                     /gene="sdaAB"
                     /locus_tag="PLM74_06275"
     CDS             complement(3333..4004)
                     /gene="sdaAB"
                     /locus_tag="PLM74_06275"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006716550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HON42721.1"
                     /translation="MNGVFDIIGPIMVGPSSSHTAGAVRLGLAGRVIMGGQPSRAALT
                     LHGSFAQTYRGHGTDVALVAGILGMRPDDERIPDSFRIAREQGMEFSFSCADLGDVHP
                     NSVLMELAGPNSSHTAVAGSSVGGGNILITRIDGFDVDFTGTSHALIVSYRDQPGVIA
                     KITSILATEDVNIAAMRVSREGRHARALAIIELDQPVSREARALITRISGIDTVIALP
                     PVVLA"
     gene            complement(4061..4195)
                     /locus_tag="PLM74_06280"
     CDS             complement(4061..4195)
                     /locus_tag="PLM74_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42722.1"
                     /translation="MTDPPGPGAVLPVQAPTPWRALSSIFLQKTSDEMQGNHVIGRIV
                     "
     gene            complement(4250..5941)
                     /locus_tag="PLM74_06285"
     CDS             complement(4250..5941)
                     /locus_tag="PLM74_06285"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HON42723.1"
                     /translation="MKCNIKGYSLTTLIIVCLLSMTILASSVAAGAVGSGAIATVSAS
                     AKATAIQFAEDNKDLTVDIAKSLWEYEEIGLDEYKSYVKARDVLAGAGFAIKQSAADI
                     PTCLVATWGLGQPVLGIYEDIDALPGVGHACGHNLNTAAGVVAAMAIKSAMESHQIPG
                     TIKVFLNPAEEVWDVAPLVAAAGYYDDVDVLLSFHAGTENTSEFGSTMAMDHVEYKFK
                     GKAAHASAAPEKGLSALDAVEIMNIAVNFLREHLIQEMRIHYVITDGGAAPNIVPATA
                     ASRYFIRAPKYPDVAYARKRIDDCAKAAALATGTELEIGFSSGIYNKVPNKSLALLAV
                     KSIESVGPAEFTDGQIAQMKALGINGIPDKEIKEPTGSQSFGSNPIGDVTWKTPSTTL
                     GIATWAPGTAGHSVEAATQSGAVYGFEGAVQASKALAALGIELLTNPESLAVVKAEFT
                     EKMKGMPPYEGKAMIPEVAYPEAPGFTVLAGDGAVKVKAVETAFVEAPGDVIVISSIQ
                     GDELASYTVSSQVDAQPEYAFKLQGGIKPGQRVKITFIDASNDGDAWFYGYVHAQ"
     gene            complement(6313..>6871)
                     /locus_tag="PLM74_06290"
     CDS             complement(6313..>6871)
                     /locus_tag="PLM74_06290"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HON42724.1"
                     /translation="VEYMVVRLGWSPDEAEERRQRYVSEYGVTLNGLHKLHGVDVGDY
                     MEYVHAVPHSEYIEPNPALDRMLGRLRPSKAIFTNASQNHARRVLSALRVSPSHFECI
                     FDFEAAGYCPKPDPEAYRRILARLGVRGDQCVLVEDNLRNLETAKSVFGMTTVLVEKA
                     DHRAKNQADLVIGDILELERVLREA"
BASE COUNT         1351 a         2122 c         1870 g         1528 t
ORIGIN      
        1 gcccggtagt agtcagcagg catgagcgtg gccggatcga taatatgatg aagaaccctg
       61 ccattgacaa tgtagtacct catgtaatcg ccgctgctgg ccactgaaga gtctttgatg
      121 aatatgacgt ccagcacatt ctgctcgtcc ggcgctatcg actcgtctgg gttctgtatg
      181 cctacgcccc aacgttcgcg gacgccatcg aagggcttcc cgattgcccg gatattgccg
      241 cctgagctta tgagcgctga cttgagccca gcttcggcca tctcacgtgc cacgatctca
      301 gtcgcatagc cctttgccac cgcgcccaca tccaggctca tctccgaatc ggaaaggtac
      361 acagtactgt tctcggtatc gattatcacc ttgtcgatat cagtgtgccg ctcagcctcg
      421 cgcaggtctt tcatgggtgg aagctcggca tgctcgggat cattctgccc ttcctcacga
      481 tagtcatgcc atattcgcag cactgctccc atagctacat tggtcttgcc acctgtgcgc
      541 ctataccagt cctttgcaaa gagcaagagg tcgattagct ccttgtccac tttcacaggc
      601 tgtacgcccg cattgtcatt aatcgtcttg acgttgttca caccctgata gtcgttgtat
      661 atgtcataca gctgatggag cttttggaaa cgcgtgcgga tcttcgcgaa atgggagttg
      721 aactcctcct cgctccgggt gtatgccacg actgtaacta gagtgttgaa gacgtcaaac
      781 aggctgtcgc tgtgcttgct atatgtgttg ctcatcctct ggcagccggc aagcgccgat
      841 gccagcaaga tcgccgcaat aaccgctgcg atgtgcctga cagttctacc cttcatttgc
      901 atcaccaact ggtattctag caggcccgct cacttcggcg caagagcagg cgctgaatcg
      961 ctgagtcgct aaaccgccac aaacctcccc catttggcat ggaggaggtt tgcacaagga
     1021 acacttgttc gcttaatatg cgtttgctct gcctacttcg cgttcgcgta ggattcagca
     1081 acagcggcaa tgtagtcgcc gacggttact gttacggtag aggtgagctc agggacatcc
     1141 ggcacagcgg gatgcgaggc gtcacgctgc ttgaccttga gtgacttgat ctccgccacg
     1201 gtcttaccga ccatccactt ctcaaactcg gcgatctgct cgaaccattc cttcttgatg
     1261 gacgaagcct tgcgcatgcc atagccgtcg cccagctcaa tcttggtctt gaactcgccg
     1321 gccttgtctg ccgcgacctt gccgtccttg tcgaatggta ccttggtctg ggcagtgtcg
     1381 attatggtgc gtacgacctt gccgtcctta tcgaacatgg tggccgcaat cgtgaggttc
     1441 acctcggcgg ctgccgcctt ggatttggca gtcgaaatat cgtgcccaag gccgagcttc
     1501 acagctcccg ccggaacgtc cacggcgttc ttgtatgcct cttcaacggc agcgatgtaa
     1561 tcctgaacgg tcatggtgac agtggaagtg agttcgggga catccggaac agatggatgc
     1621 gacgcgtcgc gctgcttgac tttgagagac ttgatctcag ccacggtctt gccaaccatc
     1681 cacttctcga acgcagcgat ctgctcgaac cattccttct tgatggagga agccttccgc
     1741 atgccgtatc catcgcccag ctcaaccttg gtcttgttct cagcggtcag atcagaagtg
     1801 actttgagat ccttgtcgaa gttcactttg gtctgggcag tatcgatgat aacctcaatc
     1861 actctgcccg ccttgtcgaa ggctactgca gccatagtcg tgtcgacctg agcaaccggg
     1921 ggagtcacct ttccattctt atctactact aggtctttgg agcttgcaat cgaggtgacg
     1981 tgtccaagcc cgactttctc tgcagactgc tggggcgacc agccaaccaa gatgaagaga
     2041 gccgcggcga gcagaagaac cgatactgct tttttcatgt ctcctcttcc tttccgatgt
     2101 gcggttttgg tgctttccat tcttgcactc tgtaccatcg tgaaccatgg cacaaacctg
     2161 gcgatgggca atgtgatctc gccctgagca agtgcagcct gtcgcaagtg acactttcag
     2221 gattcgctgc cgggattaca catttcgcct gcccacgccg ggcacaacta attataccct
     2281 ataccccctt agggtagcaa gggcaaatcg aaggtcagtc aaaggccaat caaaggcaaa
     2341 tcaaaggcgc gatgattcct tcgcgtcgtc gtgcaccgcg ttcatctccg aggcaataag
     2401 ctttccggtg ggagtggcgg ccaacccgcc gagggctgtc tctctgagcg ccgccggcag
     2461 ttcgcgtccg atcgccccca tggcccagat gacctcatcg gctggtatga cgcttcgaat
     2521 gccggccaga gccatgtcgg ctgcagccag cgctaccgcc actgacgtag cattccgctt
     2581 gatgcacgga acctctacca gtccggcgac gggatcgcat accagaccca gaagtccctt
     2641 gaaagccagc gccactccgt tggcgcactg ctcgggtgtg ccgcctgcga tctggacagc
     2701 cgcggctgcc gccatggctg cggccgcccc gcattccgcc tgacacccgc cggccgcccc
     2761 ggccagagtg ctgctgcgtg cgatgactgc gcccacaccg ccggcggcaa acagggcatc
     2821 tatgagctcg tcgtcgccgg cgccccgtat ctcgcccacc gagaacagca ccccgggaag
     2881 cactccgcat gacccggcag tgggcgccgc tacgatccgg cccatgctgg cgttcacctc
     2941 agccactgcc agcgcatagg cgatcgcgct ggcaagcggc tctcccccga tgagcacggg
     3001 agcgccgccc gctgtgccgt cagctattgc cgtgcgagtt cgggtccggg tctggctccg
     3061 ggtctgggtt cgggcctcgg ccatccgccg cgcgtctcct cccgtcaatc cgctcctgga
     3121 tcgaacgccg gcaagcccac gaccgactga atctcgcatg accgccaacc gctcctgcat
     3181 cttggagttg agcgcatcct cgggttgatc cgagtccagt gcttcccgtt cgcgcacagt
     3241 ggcgccgatt gaaagcgatc gggacgtagc agcatcagtc aactcagcaa ttgatgtgta
     3301 catctagata ctccccttcc atcccgcgcg gctcacgcta ataccacggg aggcagcgca
     3361 atgacggtgt cgatgcctga aatccgcgtg atcagcgcac gagcctccct cgatactggc
     3421 tggtcgagct caatgatagc cagcgcccgc gcgtgacgcc cttcccgcga cactcgcatc
     3481 gctgcgatat tgacgtcctc ggttgccaga atggaggtga tcttggctat cactccaggc
     3541 tgatcgcgat atgacacaat cagagcatgg cttgttcccg taaaatcgac atcaaaccca
     3601 tcgatcctcg ttatcagaat gttgccgcct cccactgatg atcccgctac cgcagtatga
     3661 gaactattcg ggcccgcaag ttccatcagc acgctgttgg gatggacatc gcccaggtcg
     3721 gcgcaggaga acgagaactc catgccttgc tcgcgggcaa tgcggaatga atccgggatc
     3781 cgttcatcat ccggacgcat tccgagaatg cctgcgacaa gagcaacatc cgtgccgtgc
     3841 cctctgtagg tctgtgcgaa cgatccatgt aacgtcagag ccgctcgaga cggctgcccc
     3901 cccataatga cgcgtccagc cagcccgagt cgcaccgcgc cggcggtgtg cgaagaagac
     3961 ggccctacca ttatcggccc gatgatatcg aaaacgccgt tcacgttggt ctgcctcccg
     4021 ttgcagtcgt ggttgcagtt gtcgttgcct gcagttaagt tcatactatt cgcccaatca
     4081 cgtggttacc ctgcatctcg tcgctggtct tctgcaggaa gatgctactc agggcgcgcc
     4141 atggagtcgg cgcctgcact ggcagaaccg cgcccgggcc aggtggatca gtcaactgac
     4201 ctgatctcct gacccgggcg cggaacggac cgaatgcccg tagcttcaac tactgagcgt
     4261 gaacgtaacc atagaaccag gcatcgccat cgttgctcgc gtcaatgaac gtgatcttga
     4321 cgcgctggcc gggcttgatg ccgccttgga gcttgaaggc gtactccggc tgcgcgtcta
     4381 cttgagacga aacggtgtaa ctggccagtt catctccttg tatcgacgaa atgacgatga
     4441 catcgcctgg ggcttcgacg aaagctgtct cgacagcttt gaccttaact gcgccgtcgc
     4501 cggccaatac cgtgaacccg ggcgcctcag ggtaggcaac ctccggtatc attgccttac
     4561 cttcatacgg cggcattccc ttcattttct ccgtaaactc cgccttcaca acggccagtg
     4621 actcaggatt ggtaagaagc tctatgccca gtgctgcgag ggccttggac gcctgcacag
     4681 cgccttcaaa gccatacacg gctccgctct gggtagcagc ctcgacggag tgtccggccg
     4741 taccaggcgc ccaggtagct atccccaggg tagtgctggg cgttttccac gtcacatcac
     4801 cgatggggtt agatccgaag ctctgacttc cggtaggttc cttgatctcc ttatccggga
     4861 ttccgttgat cccaagcgcc ttcatctgcg caatctggcc atcggtgaac tccgcaggcc
     4921 cgaccgattc tattgacttt acggccaaca gggccagcga cttgttgggt accttgttgt
     4981 atattcccga gctgaaccca atctctagtt cagtgccagt ggcgagcgca gcagccttgg
     5041 cacagtcgtc gatccgcttc cgagcgtagg ccacgtcggg gtacttgggc gcgcgtatga
     5101 agtatcgact ggcggcagta gccgggacta tgtttggagc agctccgccg tcggtaatca
     5161 catagtgtat cctcatctcc tggatcaagt gctcccgcag gaagttaacg gcgatgttca
     5221 tgatctcaac agcgtccagc gcgctcaggc ccttctccgg agcagccgaa gcgtgcgcgg
     5281 ctttgccctt gaacttgtac tccacatgat ccatggccat cgtgcttccg aactcggagg
     5341 tgttctcggt gccggcatgg aagctgagga gaacgtcgac atcgtcgtag tacccggcgg
     5401 ccgcgaccag aggtgccacg tcccacactt cctccgcagg gttcaggaac accttgatcg
     5461 tgcctggaat ctggtgtgat tccatggcgc tcttaatcgc catggcggca accacacccg
     5521 ccgctgtgtt gagattatga ccacacgcat gcccaacacc aggcagagcg tcaatgtcct
     5581 catatatgcc caggaccggc tggcctaaac cccaggtggc cacgaggcat gtgggaatgt
     5641 ccgcagctga ctgcttgatg gcaaacccgg cgccggcgag cacgtcgcgt gccttgacat
     5701 acgacttgta ctcatccagc cctatctctt catattccca gagcgacttg gctatgtcca
     5761 ctgtaaggtc cttgttgtcc tccgcgaact ggatcgctgt ggctttggcg ctcgctgaca
     5821 cggttgcgat cgcgccggac ccgacagctc cggcagcaac cgatgatgca agaatagtca
     5881 tagatagcag acagacgatg atgagggttg tgagggaata gcctttgatg ttgcatttca
     5941 ttctggatgg gactcccttt ctgtatgtag taggctatgt ccccgattat agactgtatc
     6001 ttcatacaat gcaatatcat ttctatgtaa tctctaagtc agaatcgctt gggtaggtgc
     6061 taatcgtctg aatgcgcgcc ggaaacggcg cctccttact gccgaaacgg ctgcccggcg
     6121 aatccgcagg cagaggcggg ttccatccag ataccccacc cgaagtgagg tgaagtggac
     6181 ccgtccccat ggggacgcgc ggtttcggcc atagatgaag cgaagcctgg caccaggtga
     6241 agcgcagctc cggattgctt ggtttgtggc aaaatctagg tgcctctttc cgagacacat
     6301 cgccaaacgc cgtcaggctt ccctcagcac gcgttcgagc tcgaggatgt cgccgatcac
     6361 caggtcagcc tggttcttcg ccctgtggtc ggccttctcg actagcactg tggtcatgcc
     6421 aaacactgac ttggcagtct cgaggttacg caggttgtcc tccaccaaaa cgcactggtc
     6481 gcctctcacg cccagtcgcg ccagaatccg gcgatatgcc tccggatcgg gtttggggca
     6541 gtagcctgcg gcctcgaaat cgaatatgca ttcgaaatgg gaaggtgaca cgcggagcgc
     6601 cgacagtacc cggcgcgcgt ggttctggct tgcgttagtg aagatcgctt tagacggacg
     6661 caggcgcccc agcatcctgt cgagggcggg attgggttct atgtactcgg aatgcggcac
     6721 agcatggaca tactccatgt agtcgccaac gtccactccg tgcagtttat gcaggccgtt
     6781 taaagtgacg ccgtactcgc tcacgtaacg ctgacggcgc tcctcggcct cgtccggaga
     6841 ccaccccaga cgcacgacca tgtactccac a
//
LOCUS       DAOTAC010000118         6836 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_9452_length_6836_cov_4.184191, whole genome shotgun sequence.
ACCESSION   DAOTAC010000118 DAOTAC010000000
VERSION     DAOTAC010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6836
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9452_length_6836_cov_4.184191"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="PLM74_06295"
     CDS             complement(<1..289)
                     /locus_tag="PLM74_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42725.1"
                     /translation="MCNKRCLRARWALALLAVLILLAISSSGNAASAGVGLPQAEQVA
                     GEFVTAFQKGDWKRLREISAEAALSWVEYVGARAGQTDGIQSIVRVESAAMG"
     gene            complement(326..673)
                     /gene="yajC"
                     /locus_tag="PLM74_06300"
     CDS             complement(326..673)
                     /gene="yajC"
                     /locus_tag="PLM74_06300"
                     /inference="COORDINATES: protein motif:HMM:NF014725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HON42726.1"
                     /translation="MAIAAAAAPGAASGQSLIGMLLPFVVLIGVMYFMVIRPQKTQQK
                     KREEMMAGLRKGNRIVSIGGLYGEILDIKDDALIVNVADQGERVIMRFAKWAVQDVIG
                     KDNQPKEKDQAAG"
     gene            complement(717..1841)
                     /gene="tgt"
                     /locus_tag="PLM74_06305"
     CDS             complement(717..1841)
                     /gene="tgt"
                     /locus_tag="PLM74_06305"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HON42727.1"
                     /translation="MILIPTVTLEVVKQSSDGRARAGILHTPHGDILTPVFMPVGTQA
                     SVKTVSPDELRALGARIVLGNTYHLYLRPGHDLIERAGGLHEFMSWDGAILTDSGGYQ
                     VFSLGPLRKITERGVEFRSHIDGSKHLFTPERVMEIQMAIGADIAMCFDECAPYPCDH
                     DYAAKSAAMTARWAQRCLAAHNGGRQALFGIVQGSVYADLRRKSAEDMVSLGFPGYGI
                     GGLSVGEPKDVMYAMLDETVPCLPHDKPRYLMGVGSPDALLEGVARGVDMFDCVLPTR
                     VARNGTVFTREGRIIVRDAAYAEDFRPLDPGCDCYACQNFTRAYIRHLLKAGEVLGIR
                     LTSIHNLRHLVRMMEEIRESIIDGSFDEYRKETWRVWYNN"
     gene            complement(1865..2893)
                     /gene="queA"
                     /locus_tag="PLM74_06310"
     CDS             complement(1865..2893)
                     /gene="queA"
                     /locus_tag="PLM74_06310"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HON42728.1"
                     /translation="MRVEDFDYVLPEHLIAQVPLAARDASRLMVLTRNEHRVEHRHFR
                     DLPEYLRPGDVLVLNDTRVIPARLAATRPTGGKVEILLLTPRGGDVWECLARPGRRAQ
                     VGQTLTFAGGRMTGLVLSKTEYGGRVLQLSAAAGLDVDSLVDAVGEMPVPHYIKSRLD
                     QPDRYQTVYARERGSAAAPTAGLHFTPELLRQIEQAGVVIARVTLHVGLGTFRPVKTE
                     QVEEHVMHSEHYEVSEQTAEAINSARAAGGRVVAVGTTTVRTLESAADAGGRVMPGTG
                     WTSIFIYPGYVFRATDAMVTNFHLPKSTLIMMVSAFAGREFVLDAYRRAVQEEYRFFS
                     FGDAMLIL"
     gene            complement(2922..3809)
                     /gene="era"
                     /locus_tag="PLM74_06315"
     CDS             complement(2922..3809)
                     /gene="era"
                     /locus_tag="PLM74_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004889476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HON42729.1"
                     /translation="MKSGFAAVVGKPNVGKSSLINSLVGAKVSIVSDKPQTTRNKIAA
                     IANLPDAQVVFLDTPGIHRARHNLGDRLVRTARSAMEEVDLILFVADATSSGRPEDEA
                     VASYIAEVDTPAWLLVNKIDAVRLEQLAEAEARSRRLAHFERVRLVSAATGQNMEGLA
                     CEIASAMPDGPMYYPTDVVVDRPEEFLAAELIREKLLLLTREEVPHSVAVVIDRMVRR
                     EDRDIVDIDAQVLVERESHKGIVIGAGGRVLKQAGVMARSEIERLLGSQVNLQLWVKV
                     RRRWRDDESMLDRLGFGEY"
     gene            complement(3806..4210)
                     /gene="cdd"
                     /locus_tag="PLM74_06320"
     CDS             complement(3806..4210)
                     /gene="cdd"
                     /locus_tag="PLM74_06320"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HON42730.1"
                     /translation="MGSSALRELIRAAREAREKAYVPYSGFRVGAAVLGRSGRIFTGC
                     NVENASYGATICAERTAFVKAISEGERQFDYVVVYSSVPEPVSPCGICRQFMSEFGLD
                     TPVTMVGDAGAAFTMRVRDLLPNAFQPEDLSL"
     gene            complement(4270..5385)
                     /locus_tag="PLM74_06325"
     CDS             complement(4270..5385)
                     /locus_tag="PLM74_06325"
                     /inference="COORDINATES: protein motif:HMM:NF017734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HON42731.1"
                     /translation="MTALAYRRTAPSDRPPKRRRVKYRRVRVRMGELNWLALLLLASI
                     VLLFDSLVIARQLGFIRLPGELTQLDMARSGSMAYIELARASLERDEPTGVGSAEPVL
                     SGAAETIAHARSREEIGRELAETAAGLYSSTHADRARAANERVAALVSEDARVRSHKG
                     AEAYILITPHGSNSSVQDRRGALSRDTVQAISKDPVIARSPETIELIVSASGVSFEAG
                     DPVVQAGALAREIDSLRTSIAQARQDAGLAELEGPGITVMAYDAPSGYAWDEIVHQKD
                     VLEILDSLFAAGALGAQVGGERVVATSSVRCIGPIVLVNQRPVAVNPIHINAVGNPAA
                     LERALKPVSARFAQTGKRIEIRRESSVHLSAYTRGGL"
     gene            complement(5348..6403)
                     /locus_tag="PLM74_06330"
     CDS             complement(5348..6403)
                     /locus_tag="PLM74_06330"
                     /inference="COORDINATES: protein motif:HMM:NF017734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HON42732.1"
                     /translation="MASNTKVKGTNVRRQRQWLTGIIIVLLFVNILVLARAAELITFP
                     GEVSSTALLREGAMSLADYYESQAAKLGLDRNSAVREALAKFKFEISKAGDAEGIAYI
                     IGYYGRATGDVIEREQENRRREAVLAIVNSDPRVIAVTGQAMITVSAGQDGRVVVEDP
                     TRVLSLTAIDSIQKSPALAGLAGPIDIEISAGKANVITARTMQDRIRLLRDEVDSLRL
                     SLEELKRTAGHAALTGRGIVVSMYDADGGFVNDEVVQEKDIRDVVNELFAAGAQGIEV
                     GGQRLVATSSIRSAGAQVLVNQRPIPVNPVVVRAVGDTQVLESSLDLIRNSLKRWGIR
                     VEVERYDSLSLPPYRAQ"
     gene            complement(6404..6787)
                     /locus_tag="PLM74_06335"
     CDS             complement(6404..6787)
                     /locus_tag="PLM74_06335"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HON42733.1"
                     /translation="MKARNLGESFRFAGIGMVQAFRSERNIRIHIAAAVTAVLLSHVL
                     GLGRLETAIVVLATVIVIVAEMVNTALEHLVDLVTAEYHPLAAKVKNIAAGAVLTAAI
                     GAAIVGIIIFGPHLVSMISRGEVKP"
BASE COUNT         1343 a         2293 c         1842 g         1358 t
ORIGIN      
        1 cgcccatggc ggcagactcg acccgcacta tggactggat tccgtcggtc tggcctgcgc
       61 gagctcccac atactccacc cacgaaaggg ccgcctcggc cgaaatctcc ctgagccgtt
      121 tccaatcgcc cttctgaaat gccgtcacga actcacctgc cacctgctcg gcctgtggca
      181 gcccgacgcc ggcggaggcg gcgttgcccg atgacgaaat cgcaagcaaa atcagcactg
      241 ccagcagggc cagcgcccat ctggcccgta aacatcgttt attgcacata tgtacgcccc
      301 cttccgaacg tataaccaga caacgctatc ctgctgcctg gtctttctcc ttgggctggt
      361 tatcctttcc gattacgtcc tgcacagccc atttcgcgaa acgcatgatg accctctcgc
      421 cctggtcagc cacattcaca attagggcgt catctttgat gtcgaggatc tcgccgtaga
      481 gcccgccaat ggatacgatg cggttaccct tgcgcagccc cgccatcatc tcttcgcgtt
      541 tcttctgttg agtcttctgg ggccttatga ccatgaagta catgacccca atcagcacca
      601 cgaacggaag caacataccg atcaggctct gacctgatgc agctccagga gccgccgcag
      661 ccgctattgc cataagacta ttcctccatt ccctgtccgg cttttgtccg agttgcctag
      721 ttattatacc agactcgcca cgtttccttc cggtactcat caaaacttcc gtctatgatg
      781 gattcgcgga tttcctccat catccggacc aagtgcctca agttgtgaat ggatgtaagg
      841 cggatcccca gaacctcccc ggccttcaac aggtgccgga tataggccct ggtgaagttc
      901 tggcacgcat agcagtcaca ccccggatcg agcggacgga agtcctctgc atatgccgca
      961 tcgcgcacga tgatccggcc ttcacgcgtg aacacggtcc catttcgcgc gaccctggtg
     1021 ggcaacacac aatcgaacat gtcgacccca cgcgccaccc cctcaagcaa ggcgtcaggc
     1081 gaacctacgc ccataaggta tcggggcttg tcatgaggca ggcagggcac cgtctcatcc
     1141 agcatggcat acatcacatc cttgggctct cccaccgaca gcccaccgat gccatagccc
     1201 ggaaatccca gcgagaccat atcctctgcg gacttccgtc tgaggtctgc atacaccgac
     1261 ccctgcacta tcccaaacag cgcctgcctg cccccgttgt gcgctgcaag gcaccgctgg
     1321 gcccagcgag ccgtcatcgc cgccgacttc gccgcatagt catgatcgca cggatatgga
     1381 gcgcactcat cgaagcacat ggcgatatcg gcgcctatgg ccatctgtat ctccatcacg
     1441 cgctcaggtg tgaacaggtg ctttgatcca tcgatgtgcg aacggaactc gactccccgc
     1501 tccgtgatct tgcgtagcgg ccccagcgag aacacttgat aaccaccact gtccgtcaag
     1561 atggctccgt cccacgacat gaactcgtgt aagccgcccg ctcgctcgat caggtcatgc
     1621 cccggcctaa ggtagaggtg gtacgtgttg cccaggacga tgcgggcgcc caaggccctg
     1681 agctcgtcag gcgagactgt cttcaccgag gcctgagtgc cgacgggcat gaacaccggc
     1741 gtcaggatgt ccccgtgcgg cgtgtggagt atacccgctc tggcccgccc atcggaagac
     1801 tgcttcacta cttccaaagt cactgtcgga atcaatatca cccctactgc ctccggcgat
     1861 gtcgctacag aatcagcata gcatcgccaa aggagaagaa cctatactcc tcctgcactg
     1921 cgcggcggta cgcgtcaagg acgaactcgc ggcctgcaaa tgccgacacc atcatgatca
     1981 gcgtcgactt gggcaggtgg aaattggtga ccatcgcgtc ggtggcgcgg aatacatagc
     2041 cgggatatat gaagatgctg gtccagcccg tgccgggcat cacccggccg cccgcgtccg
     2101 cggcgctttc caacgtgcgt acagtggtcg tgcccaccgc aactacgcgt ccccctgccg
     2161 cgcgggcgga gttaatggcc tcagcagtct gctccgacac ttcgtagtgc tcggaatgca
     2221 tgacatgctc ctcgacctgc tccgtcttta ccggtcgaaa cgtacccagg cccacatgaa
     2281 gcgtgacccg ggcgatcacc acacccgcct gttcgatctg ccggagcagc tctggggtga
     2341 aatgcagccc cgcagtgggg gcagcggccg atccgcgctc ccgcgcatac accgtctggt
     2401 agcggtcagg ctggtccagc cggctcttta tgtagtgagg cacaggcatc tccccgacag
     2461 cgtcgaccag agagtcgacg tccagccccg cggctgcgga aagctgcagc acccgcccgc
     2521 catactccgt cttgctcagc acgagccctg tcatgcgtcc gcctgcgaac gtcagggtct
     2581 ggccaacttg agcccggcgg cccggcctgg ccaggcattc ccatacatcc ccgcccctag
     2641 gggtgagcag aagaatctcc accttgccgc ctgtgggtcg cgtagcagcg aggcgcgccg
     2701 ggataactcg cgtatcgttc agcaccaata catcgccggg cctaaggtat tcaggaaggt
     2761 cccggaagtg cctgtgctcc actcgatgct cgtttcgcgt cagtaccatg aggcgcgaag
     2821 cgtcccgcgc ggccagggga acctgagcaa tcaagtgctc aggcagtacg taatcgaagt
     2881 cctcaacgcg catatgcgaa tcctttcccc aaaagaggcg tctagtactc accaaacccc
     2941 agtctatcca gcatagactc atcatcgcgc cacctgcgcc ggactttcac ccacagctgc
     3001 aggtttactt gcgatcccaa gagcctctca atttctgacc tggccatcac accggcctgc
     3061 ttaagcacgc gtccgcccgc gccgatcacg attcccttgt gcgattcccg ctccacaagc
     3121 acctgagcgt cgatatcgac gatgtcgcgg tcctcccgac gcaccatcct gtcgatgaca
     3181 acagccaccg agtgcggcac ttcctcgcgc gtaagaagca gcagtttctc gcggatcagt
     3241 tcggccgcga ggaactcctc cggcctgtcc accaccacat cagtcggata gtacatgggc
     3301 ccgtcgggca tcgccgacgc gatctcgcaa gcgaggccct ccatgttctg ccctgtcgca
     3361 gccgacacca gccgcacgcg ctcaaagtgc gccagccgtc gtgatctggc ctcggcctcc
     3421 gccagctgtt ccagacgaac agcatcgatc ttgttgacca gaagccatgc aggtgtgtct
     3481 acttcggcta tgtatgatgc tacggcttcg tcctcaggtc gcccactaga tgtcgcatcg
     3541 gcgacaaaga ggattaagtc gacctcttcc atggcggacc gagccgtgcg caccaaacgg
     3601 tcgcccagat tatgacgggc tctgtgtatt ccgggagtgt ccaggaacac tacttgagca
     3661 tcgggcagat ttgcgatcgc cgcaatcttg ttgcgggtag tctgaggctt gtccgataca
     3721 atggacacct tcgccccgac aaggctattg atgagagacg acttgcccac gttgggcttc
     3781 ccgacgacgg ctgcaaatcc ggacttcaca ggctgagatc ctccggctgg aatgcattgg
     3841 gcagcagatc gcgcactctc atcgtaaatg cggcgccggc atcgcccacc atggtcaccg
     3901 gcgtgtcaag gccgaattcc gacatgaact gccggcagat cccgcatggg ctcacaggct
     3961 ccggcacact ggagtatacc actacgtaat cgaattgtcg ctcgccttcg gatatggcct
     4021 tcacgaaggc agtgcgctcg gcacagatcg ttgcgccata tgaagcgttc tccacgttgc
     4081 agccggtgaa gatgcgaccg ctcctgccca gaaccgccgc acccacccgg aatccggagt
     4141 aggggacgta cgctttctcg cgggcctccc tagctgcccg gatcagctcg cgtagggcag
     4201 agctacccac tgccgctgcg gcttcactga tgtcagcttc tcggaccccc tggtctgggt
     4261 tgcccaactc tacagacctc ctcttgtgta tgcgctgagg tggacggagg actcgcgacg
     4321 gatttcgatg cgctttcccg tctgcgcaaa tctcgccgac accggcttaa gcgcccgctc
     4381 cagcgccgca ggattgccca cagcattgat gtgtatgggg ttgactgcga caggacgttg
     4441 gttcactagg actattggcc ctatgcacct caccgatgac gtggccacta cccgctcccc
     4501 acccacctgc gcgccgagtg cgccggcggc gaagagcgaa tccaatatct ccagcacgtc
     4561 cttttggtgg actatctcgt cccaggcata ccccgacggc gcgtcgtacg ccatcacggt
     4621 aataccaggt ccctccagct cagcgaggcc cgcatcttgc cgtgcctgag ctatggatgt
     4681 acgcagagag tctatctcgc gagctaaggc ccctgcctgt accacggggt cgcctgcctc
     4741 aaatgacacg cccgacgcgc tcacaatgag ctcaatggtc tcaggcgacc tcgcgatgac
     4801 ggggtctttg gagatggcct gcacggtatc gcgggataag gcgccccgtc gatcctgaac
     4861 agaggaattg gagccatggg gcgtaatgag aatgtacgcc tccgctccct tgtggcttcg
     4921 cacgcgcgcg tcctccgaca ccaaagcggc cacacgttca tttgccgctc gtgcccggtc
     4981 cgcgtgtgtc gacgagtaca gcccggcggc cgtctcggca agctcgcgac cgatctcctc
     5041 acggctgcgc gcgtgggcta ttgtctcagc tgcacccgaa agcaccggtt cagccgatcc
     5101 cacgccggtc ggctcatcgc gttccaacga cgctctggcc agttcgatat aggccatgga
     5161 tccgcttcgg gccatgtcga gctgcgtgag ctcgcccgga agacgaatga aaccaagctg
     5221 cctggctatg acgagcgagt cgaagagcag tacgattgac gcaaggagca gtagggcaag
     5281 ccagttaagc tcgcccatcc tcacccggac tcgccgatac ttgacacgcc gccgtttggg
     5341 cggacggtca ctgggcgcgg tacggcggta ggctaaggct gtcataccgc tccacttcca
     5401 ctcttatccc ccaccgcttg agggagtttc tgataaggtc gaggctactt tcgagcacct
     5461 gcgtatcccc taccgccctg accaccacag ggttgaccgg aatcggcctc tggttgacga
     5521 ggacttgagc acccgccgac cttatcgatg aggtagcaac caatcgctgc ccgcccacct
     5581 cgatgccctg ggcgcccgcg gcgaacagct cattcactac gtccctgata tccttctcct
     5641 gaacaacctc gtcattgaca aaaccgccgt cggcatcgta catcgatact actattccgc
     5701 gccctgtgag ggctgcatgg cccgctgtgc gcttgagctc ttccagcgac agacggagcg
     5761 aatcgacctc gtcgcggagg agacgtatcc gatcttgcat ggttcgagcc gtgataacgt
     5821 tggctttgcc tgccgatatc tcgatatcaa tgggccctgc caggccggca agagccgggc
     5881 ttttctgtat ggagtcgatg gccgtaagcg acagcacacg agtgggatcc tcgacaacta
     5941 cccgcccgtc ctggcccgcc gacaccgtga tcatggcctg cccggtcact gctatcactc
     6001 tgggatcgga attgacgatg gcgagcacgg cttcgcgccg cctattctcc tgctcacgct
     6061 cgatgacatc gccggtggcg cgcccgtagt agccgatgat gtatgcgatg ccctccgcgt
     6121 cgcccgcctt ggatatctcg aatttgaact tggcaagagc ctcccgcact gcactgttgc
     6181 ggtccagccc cagcttggcg gcttgcgact catagtaatc cgctagcgac atggctccct
     6241 cgcgcaaaag agcggtactg gagacctcgc ccgggaaagt gatcagctcg gcggcacggg
     6301 ccagcaccag gatgtttacg aacagaagca ctattatgat gcccgtgagc cactgtcgct
     6361 gcctgcggac gttcgtgccc ttcactttgg tgttggaagc catctacggt ttcacctcgc
     6421 ctcgcgagat catggatacc aagtgcgggc cgaagattat tatgccaact atagctgccc
     6481 ctatcgccgc cgtcagaacg gcccctgccg ctatgttctt caccttggcc gccagcggat
     6541 ggtactctgc agtgaccagg tcgacgagat gctccaatgc tgtgttgacc atctccgcga
     6601 ctatcacgat cacggtggcc aacaccacga tagcggtctc cagccttcca aggcccaata
     6661 cgtgcgagag caacactgcc gtcacagccg ccgcgatgtg tatgcggatg ttgcgctcgc
     6721 ttctgaatgc ctgaaccatt ccgatccctg cgaatctgaa actctcaccc aagttacgag
     6781 ctttcaaact gcccgccccc acatgctaca ttgcatacgt caggaggctg tagcta
//
LOCUS       DAOTAC010000119         6786 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_9558_length_6786_cov_4.261180, whole genome shotgun sequence.
ACCESSION   DAOTAC010000119 DAOTAC010000000
VERSION     DAOTAC010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6786
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9558_length_6786_cov_4.261180"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="PLM74_06340"
     CDS             complement(<1..346)
                     /locus_tag="PLM74_06340"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HON42734.1"
                     /translation="MNVQLELDRSLMREAIAEARKAASAGEAPVGAVIVLGGEIIARA
                     HNLKESLGDPTAHAEVLAIREAAGALGAWRLSGATLYVTLEPCPMCAGAIVQARMERL
                     VYGAYDPKAGAAG"
     gene            429..1181
                     /locus_tag="PLM74_06345"
     CDS             429..1181
                     /locus_tag="PLM74_06345"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HON42735.1"
                     /translation="MSRFPSERDTLKSKFPRMREEPTDQRLGLPQPPLQKAYAADAVL
                     IDLPSPREATPRSVNLFDLMERRQSRREYSTEPITLAELSFLLWATQGVKRVVRDGFA
                     TMRTVPSGGSRHAFETYLAANRVDGLEPGIYRYLALEHKLIRVSSPPDLPAQLGKACF
                     GQDYVGTAAVVFFWSCIPYRCEWRYAVAAHKTLAQDSGHVCQNLYIACEALGLGTVAI
                     GSYHQETADALLGLDGTDEFVIYAAPVGRVNP"
     gene            1373..1663
                     /locus_tag="PLM74_06350"
     CDS             1373..1663
                     /locus_tag="PLM74_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HON42736.1"
                     /translation="MAYEDKTLNCRDCGRDFVFTAGEQEFYVQRGFQNEPSRCPDCRR
                     ARKSMNNSGNRQMYEAVCSSCGKPAYVPFQPSGDKPVYCSECFAAQRASRQY"
     gene            complement(1831..3525)
                     /locus_tag="PLM74_06355"
     CDS             complement(1831..3525)
                     /locus_tag="PLM74_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42737.1"
                     /translation="MSLDWRHLSRTRLAQAIVWGLAVAAVVSCASVGVFAQIDFTFAP
                     MVVNLEVSPGGTGVFEAAIKNESTAKTGEFIVHVMDVVQLPNGEYGLVERGTAPSSCS
                     SWIKLSTDRVSVGPGQWGQVDALVEVPRGVYGGRYAALVFELVPDRDQSGQVWGSSTV
                     TPNIMTVVELSVPGPLVRKSLGVTGFFVRSGREDPELAMRYGESAVMLSAEVANDGNT
                     HVFANGSIILRDANGRRLKQIPLGGGRGIVLPGSSARLNSVFVAGIPPGEYIADVSIT
                     YGGLRPASAKVSFTVISGDASTSTIGGGGKIPPFTVDPALVELNYPPGATAARAFVIE
                     NKSPEVMQVQGRVLPLIYDEYGELVEEGSEEDSNSCASWVELRPQQVTLSPGGKQVVR
                     ALISIPRDATGSRYANITFTGIPAGDADAQDRPAWAGETGSMLFLTIGKNHVKQADLG
                     SLQVFDSGGIGGYVLEAVFRNIGSVHVVPQAVFVIKKRTMPQSVPGVEYIGPGTLSEV
                     ARVDLGYSDTPVLPGQMRFLVVDYPYSLDPGEYVAEVIAEYGGQAPVSCSQEFTVP"
     gene            complement(3612..4340)
                     /locus_tag="PLM74_06360"
     CDS             complement(3612..4340)
                     /locus_tag="PLM74_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42738.1"
                     /translation="MVRKLVVCLAIIATLVVPAVTWAATPSYVSTHDLPADATRQAHW
                     EYNPATNTWVKYAANSSGNNARGWISGKQYNEFTCCNGPQWELTITNHASIAHWCKWK
                     MSATRWDWRIRKPGKYIMDGLVLEIYSNQDITLKHEGFGNLICTGDLWKGEVRPEIAA
                     WYTWEPLQANLPDESHWKTGAAFNGDMTVPFNRWPNAWGWEGHLFTKLEVDNFAQSCE
                     YERTSKITIKVVNQKDWLENGTWK"
     gene            complement(4563..5939)
                     /locus_tag="PLM74_06365"
     CDS             complement(4563..5939)
                     /locus_tag="PLM74_06365"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HON42739.1"
                     /translation="MATSRLKNVLFPTVMAWKAYAVVIVAALLPAAVIAAAGVPLFGT
                     VERQARERELRSICRLLDLELTERFSSMQTILEAAGAVDLPPEAQARALHTALQPVVD
                     RVCSAYPGVEVGYYSRALGLEVAAGRDLNARPFFRTRPPEDFRVYQTGRPEIAYDNSD
                     RRILGARVTATQVYPLYDGAELTGHVWASVEARQLIPNTVAAAMSVFAGAAASAVTML
                     VAAWWLMNALQGWEREAAARRTTAANQLAAEVVHELRNPISVARGLAQLVMSEDDDLR
                     RGMWMGNLVRQLDHMNTIASDYLRYARPAEPVLRSVPVGEILGELENLMRSSVEEAGV
                     DLEVSHGSSALEVMCDPEQLNQVLINLVKNAVEAVSGESPARVCVRARQDGRDVLIDV
                     CDSGPPVRQEDLPHLFEPFFTTKSKGTGLGLAISRRLVEAQGGLLSAFSGEHGVTFSI
                     RLPMSSIA"
     gene            complement(5963..>6786)
                     /locus_tag="PLM74_06370"
     CDS             complement(5963..>6786)
                     /locus_tag="PLM74_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42740.1"
                     /translation="AAILAGPASALELPYPPNARLMAMGWTFVGVVDQHDPGRSNPGA
                     YGFNSDQITLSLGAGFGAGLDAFSTVYAGVSDLDRGVGGGGLALVYSKAEEGEHDLKY
                     ATYGIGRKVADWCSLGLSLAYAQQSDSERAALTTDTGLLLKIGTLSAGVQASSITTSL
                     VGGGVKMETEFTPEISVGLAFAPSKALTVAVDAHGVVKHDDGAQPWYSGGAEVWLRDK
                     LALRVGAFGSFAEGAADLSYTGGVGIKMGKAEVGYALVWSDGGLRAQCFTASSSF"
BASE COUNT         1378 a         2215 c         1844 g         1349 t
ORIGIN      
        1 tgccggccgc tccggccttg ggatcgtacg ctccgtacac caaccgctcc atccgcgcct
       61 gcactatcgc accggcgcac atcgggcacg gctcaagcgt cacatacagc gtcgcgccgg
      121 aaagcctcca tgctcctaga gcacctgcag cctcccttat cgccaagacc tcggcgtgtg
      181 ccgtggggtc gcccagcgac tccttcaggt tgtgcgccct ggcgataatc tcgcctccca
      241 gcacaatcac cgcgcctacc ggggcctccc cggcggaggc agccttccgc gcctcggcaa
      301 tcgcctcgcg catgaggctt ctatccagct ctagctgcac gttcattttc aagcgcacac
      361 ccttgccagg agatatgtta ccatagcagt gaagcgaaat caacaacgta agccggaggc
      421 tgagaagaat gagcagattc cccagtgagc gcgacaccct gaagtccaag tttcccagaa
      481 tgcgggagga gcccacggac cagcgcctgg gtctccctca gcccccgctg cagaaggcgt
      541 atgccgcaga cgccgtgctc atcgatcttc catcgccgcg cgaggccact ccgcgctcag
      601 tcaatctatt cgatctgatg gagcgccgcc agagccgccg agaatactca accgaaccga
      661 tcaccctggc tgagctctcg ttcctcctct gggccaccca gggagtcaag cgagtagtca
      721 gggacggatt cgccaccatg cgcacggtgc cttcgggcgg gtcgcgtcat gctttcgaga
      781 cgtatctggc cgcaaaccgc gtcgacgggc tcgagccggg catctaccgg tatctcgccc
      841 tggagcacaa gctgatccgc gtatcatcgc cacccgacct cccagcgcag ctgggcaagg
      901 cgtgttttgg gcaggactat gtgggcacag ccgcggtggt gttcttctgg agttgcatac
      961 catataggtg cgaatggcga tatgccgtgg ccgcccataa gacgctggcc caggactcgg
     1021 ggcacgtctg ccagaaccta tacatagcct gcgaagcgct gggcctgggc accgtagcga
     1081 tcgggtcgta tcaccaggag acggcagacg cgctcttggg acttgacggc actgacgagt
     1141 tcgtgatcta cgccgccccg gtgggacggg ttaaccccta gcggcgcaag tgcctgtgca
     1201 cgactgccgc gcgaccaaaa acagctcgaa ttcgggcatg tctttgctgc catcatattg
     1261 caattggttc acatatgctc tattatatac atggctgttg ttcaagcgca cgcactttca
     1321 agcatgctga cttgatcaat agactatcca gaccaagtga ggcgtgatca agttggcata
     1381 cgaggacaag accctgaatt gccgcgactg tggcagggac ttcgtattta ccgccggtga
     1441 gcaggagttt tacgtgcagc gtgggttcca gaacgagccg tcgaggtgcc ctgactgccg
     1501 tagggcgcgc aagagcatga acaactcggg caaccgccag atgtacgaag cagtttgctc
     1561 gtcttgtgga aagccggcat acgttccctt ccagcccagc ggcgacaagc cggtctactg
     1621 cagcgagtgc ttcgcagcgc agagggccag caggcagtac taatcgcatc tgctggcgcg
     1681 taaggcgccg cgagcagctt gcgataagcc gagccatcca agccccggag gatgtgaatc
     1741 cacggggctt tgaatttatg ttcgtctggt cctggctcag gttcgcgatg catcggtttc
     1801 tgcctatgta ggcagtgcgc aaccttcccg ctacggcaca gtgaactcct gggagcatga
     1861 tacgggcgcc tgcccgccat actctgcgat tacctccgcc acgtattcgc ctggatcaag
     1921 ggaatatggg taatcgacta caaggaagcg catttggccg ggcaacacag gcgtatcaga
     1981 gtagcccaga tccacccgcg caacctctga cagggtacca gggcctatgt actccactcc
     2041 gggcacgctc tgcggcatgg ttctcttctt gatcacgaac accgcttgtg gcacgacatg
     2101 aacgctgcca atgttcctga agacagcctc tagaacatat cctcctatgc cccccgagtc
     2161 gaatacctga agcgagccaa ggtcggcttg cttcacgtgg ttcttgccta tggtcaagaa
     2221 cagcatggat ccagtctcac cggcccacgc aggcctgtcc tgagcatccg cgtcgccggc
     2281 gggtatgcct gtgaatgtga tgttggcgta tctggacccg gtagcatccc gtggaatcga
     2341 gatcaaggca cgtaccacct gcttgccccc tggcgacagc gtgacttgct gaggacgcag
     2401 ttcgacccaa ctcgcgcagc tgttggaatc ctcttcgctt ccttcttcca caagttcgcc
     2461 atactcatcg tagataagag gaagcacgcg tccctgaacc tgcatgactt cgggagactt
     2521 gttctcgatc acgaacgctc gagccgctgt agctccgggc gggtagttca gctcgacaag
     2581 agccgggtcc acggtaaacg gggggatctt gcctccgccg ccaatggtcg aagtcgacgc
     2641 atcgcccgat attacggtga aactgacctt tgccgaagcc ggcctaaggc cgccataggt
     2701 gatcgaaaca tcagcaatgt actctccggg cggaatgcct gccacaaata ctgagttcag
     2761 ccgagcagag gaaccgggaa gcacgattcc ccggcctcct cccaagggga tctgcttcag
     2821 ccgacgaccg ttggcgtctc tcaggataat gcttccgttt gcaaagacgt gcgtatttcc
     2881 gtcgttggcg acctcggccg ataacatcac cgcactctcg ccataacgca tcgccagctc
     2941 agggtcttcc ctcccggacc gcacgaagaa acctgtaacg ccaagagact tcctcacaag
     3001 agggccaggt actgaaagct cgactacggt catgatgttt ggcgtcacgg tgctggaccc
     3061 ccacacttgg ccgctttgat ccctgtcagg caccagctcg aacaccaagg cagcgtatct
     3121 tccaccatat actcctctgg gcacctccac tagggcatcg acttggcccc attgcccagg
     3181 ccctacactc actctgtcgg tagatagctt gatccagcta gaacaggaac taggcgccgt
     3241 accacgttcc acaagaccgt actctccatt gggaagctga acaacgtcca tgacgtgcac
     3301 gatgaactcg ccagtcttcg ccgtactctc gttcttgatt gccgcctcaa acacgcctgt
     3361 gccgccgggc gatacctcga gattcaccac catgggcgca aaggtgaaat cgatctgagc
     3421 aaacacacct actgaggcac acgatacgac cgccgcgaca gctagtcccc agacgatggc
     3481 ctgggcaagg cgcgtgcggg atagatgccg ccagtctaga ctcatgcctg tccctccata
     3541 ttctgtcatg cggggcgacc ttgaagatcg ccccgcgtgt cgcgctgacc cagatcggtt
     3601 actcacttgc actacttcca ggtgccgttt tcaagccaat ccttctggtt tacgactttg
     3661 atagtgatct tggacgtcct ctcgtactcg cacgactgcg cgaagttgtc aacttctagc
     3721 ttggtgaaca ggtgtccttc ccaaccccac gcattcggcc acctgttaaa tggtaccgtc
     3781 atgtccccat tgaaggcggc cccggtcttc cagtggctct cgtccggtag attggcctgc
     3841 agcggttccc acgtatacca tgcggctatc tcgggtctca cttcgccctt ccacaggtcg
     3901 cctgtgcaaa ttaggttgcc gaagccttca tgcttgagtg tgatgtcctg attgctgtag
     3961 atctccagca ccagtccatc catgatgtac ttcccaggct tcctgattct ccaatcccat
     4021 cgagtcgcgc tcatcttcca cttgcaccag tgcgcaatag atgcatggtt agtaatcgtc
     4081 agttcccact ggggtccgtt gcagcaagtg aactcattgt actgcttgcc gctgatccag
     4141 ccacgcgcgt tgttgccgct actattggcg gcgtacttga cccaagtgtt cgtcgcgggg
     4201 ttgtactccc agtgcgcctg cctagtcgcg tcagcgggaa gatcgtgtgt gctgacgtag
     4261 gatggagtcg ccgcccacgt cacggccggc accacgagag tcgcaatgat cgcaagacag
     4321 actacgagct tcctcaccat tgatccgccc cccaacatga tttcgatcgg acctggtatc
     4381 agaggctcag gagctcacga tacgcatgtc gagtctaggg gcgctccgac atgtcaggct
     4441 ccgatcagaa caccatatga ttgtcctatc gaactcatga tagggacagg gaagagaaat
     4501 agcgacacaa ggccccgtgg aaatcgggga tcaacgtagc cataggtggg ccgggtcctc
     4561 gctcaagcta tgctgctcat tggcaaccga atggagaacg tgacgccatg ttctccgctg
     4621 aaagcagaca gaagaccgcc ctgagcttcc acgagacgcc ttgatatggc aagccccagg
     4681 ccggttccct ttgacttggt tgtgaagaaa ggttcgaata ggtgcggtag gtcctcttgg
     4741 cgaactggcg gtccggaatc acacacatct atgagtacat ctctaccgtc ttgcctcgcc
     4801 cgcacgcaca cccgtgccgg ggactcgcct gagaccgcct ccaccgcgtt cttcaccaga
     4861 ttgatcagaa cctggttgag ctgctccgga tcgcacatca cttccagggc gcttgacccg
     4921 tgcgatacct caagatccac cccagcttcc tcgacacttg acctcatcaa gttctcaagt
     4981 tcgcccagga tctcgcccac aggcacactc cgcagcaccg gctcggccgg ccgggcgtag
     5041 cgcaggtagt cggaggcgat ggtgttcata tggtccagct gccttacgag attgcccatc
     5101 cacatcccgc gccgcaggtc atcatcttct gacatcacaa gctgcgctaa gccccgcgcc
     5161 accgagatag ggtttcgcag ttcatgtaca acctccgccg ccagctggtt ggccgccgtg
     5221 gtccgcctgg cagccgcctc tcgctcccat ccctggagcg cattcattag ccaccatgca
     5281 gccacaagca tcgtaactgc gctagccgca gcccccgcaa acacgctcat tgccgcggcc
     5341 acagtattcg ggatcagctg tcgcgcttcc acactggccc acacatgtcc cgtgagctcg
     5401 gccccgtcat atagaggata gacctgcgtc gccgtcacgc gggcgcccaa gatcctgcgg
     5461 tcggagttgt cgtaggcgat ttcgggtcgc cccgtctggt acactctgaa gtcctcagga
     5521 ggacgcgttc ggaagaacgg ccgcgcgttc agatctcgtc cggcggctac ctcaaggcct
     5581 agcgccctgg agtaatagcc tacctccacc cctggatagg ccgagcagac cctgtccacc
     5641 acaggctgga gcgcagtgtg cagcgcccgg gcctgcgctt cgggcggcag gtccaccgcg
     5701 ccagccgcct ccaggatcgt ctgcatggag ctgaacctct cggttagctc caagtcgaga
     5761 agccggcaga tagagcggag ttcacgctcc cgcgcctgtc tctccaccgt gccgaacagg
     5821 ggcacgccag ctgcggcgat gaccgccgcg ggaagcagag ccgcgacgat caccaccgcg
     5881 tatgccttcc acgccatcac tgtggggaac agtacgttct tgagcctgct cgtagccaag
     5941 gccaacaccc ctgactgcag ggtcaaaagg acgagctagc cgtgaagcac tgcgctctca
     6001 ggcctccgtc gctccacacc agcgcatacc cgacctcagc cttgcccatc ttgatcccga
     6061 cccctccggt gtacgacaga tccgctgcgc cctctgcaaa cgagccgaac gcccctaccc
     6121 ggagcgctag cttgtcccgg agccacacct cagcgccgcc ggaataccac ggctgcgcac
     6181 cgtcatcatg cttcaccacg ccgtgggcgt cgacggccac agtgagagcc ttggaaggcg
     6241 caaaggccag tcctaccgat atctccgggg tgaactcagt ctccatcttg accccaccgc
     6301 cgacaaggct tgtggtgatg gatgatgctt gcaccccagc ggacaaggtt ccaatcttca
     6361 gcagaagccc tgtgtcggta gtcagagccg ctctttctga gtcagactgc tgtgcatatg
     6421 ccagtgacag accgagcgaa caccagtcgg cgaccttcct accgattccg tatgtcgcat
     6481 atttcaggtc gtgctcgccc tcttcagcct tggagtagac cagggccagt cctccgcccc
     6541 ccactccgcg gtcaagatcg ctcacgccag cgtagacagt gctgaacgca tcaaggccgg
     6601 cgccgaaacc agctcccagg ctaagtgtaa tctggtcgct gttgaacccg taagctcccg
     6661 gattggacct gcccggatcg tgctgatcga caacccccac aaaggtccag cccatcgcca
     6721 tcaggcgcgc attcggagga tacggaagtt ccagagcaga ggcaggccca gcgagtatcg
     6781 ccgccg
//
LOCUS       DAOTAC010000120         6769 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_9593_length_6769_cov_3.395293, whole genome shotgun sequence.
ACCESSION   DAOTAC010000120 DAOTAC010000000
VERSION     DAOTAC010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6769
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9593_length_6769_cov_3.395293"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="PLM74_06375"
     CDS             <1..292
                     /locus_tag="PLM74_06375"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HON42741.1"
                     /translation="MIGSYAVNSSMWDVGVCLGFGVIGWLLKRYGYSLPPLVLGMVLG
                     GMAEVNLRRATMMFGPTAIFQRSGSVILLLISALSFAIPVLQARRAKTKART"
     gene            337..1242
                     /locus_tag="PLM74_06380"
     CDS             337..1242
                     /locus_tag="PLM74_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42742.1"
                     /translation="MVRGKHRASRWLGTLIALLALASLAPTAAETTMVAPDSRELKLL
                     IQTEVFADRADGYARCWGIVKDLAAETGVQATEAKDPFAEGARKVMYLDTHSGRLDQE
                     NRSLRLRIKLKDGKLDDKVDLNLKYRRSDVDSVPSDAVTAAAGIKASFSYEEDVAGFI
                     GGVVGQNSSAASIACTVKGVAADKLTGNTLAVYAEYFPSLAVLGIPLDSPLEMVGGIA
                     IREHKVTPGELDFGQGMKTEVDISVWYNFDTGEIITAEISYGSALGSEAPAAAVAKAE
                     AFFNALQERMAGILAPGGMKTDLLR"
     gene            complement(1993..2565)
                     /locus_tag="PLM74_06385"
     CDS             complement(1993..2565)
                     /locus_tag="PLM74_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HON42743.1"
                     /translation="MSRYGKQRYDAPGIRGGIFVVRGDITEQRVDAIVNAANTTLLGG
                     GGVDGAIHLAAGPDLLAECRTIGGCTTGDAVITRGYRLPARYVIHTVGPIWQGGNAGE
                     AELLADCYINSLKLASEHGVRTLAFPSISTGAYGYPVSQAAQIAIRAVTDYLQTDPSI
                     DEVRFVCFDEHTFDAYEQALKTACPPWASI"
     gene            2709..4172
                     /locus_tag="PLM74_06390"
     CDS             2709..4172
                     /locus_tag="PLM74_06390"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HON42744.1"
                     /translation="MEYRVRREPMGVSADGVTLFPAMLSRVIAAVDRRRLVAVVLLLL
                     AVILGLTILRHTRVPSWAGDMETDYCTLVIDQAAASLTAFLKDYEDATFQFVANQDLN
                     RLLLEYVSATDTYEVAPYNQAFSNFLEGYTFSRRGVYDAVFVDESNRNRKALTMRESL
                     PASYIKAFRNSGTYEEIMRADGKAVWAFGEDCDRAGMNCVIVGRRIKHLYTQKPLGIV
                     AIMIDADELARVVNDYLHDHFYFSVGTVRANYTVIVDREGCIISGPSAPGPSRDEKPS
                     FLRELYPNPPVGRAEGRFTGTVRGEDVLVIFRPISDTSWRAFVPVSLAGLYGEEPGGG
                     ASAVRLSIPMAGGLIAGALAATAVFLFLWPSPRSPRSPHGPYSPHIYSPGRNGACVDE
                     GLAGARGATEAGRLQPEGPDPEWLGQLTDKEIQVLILMSQGYSNREIAQKTYIAQQTV
                     KNYVSCIYSKLCVRDRVQASLLAVEAGLNLRDYRDLD"
     gene            complement(4205..5212)
                     /locus_tag="PLM74_06395"
     CDS             complement(4205..5212)
                     /locus_tag="PLM74_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012612712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HON42745.1"
                     /translation="MKRRPAVRAVLLALSLLAVVMAPWADAAPKSRLVVYTSTKDVII
                     GQLRDTFMRKYPEIQLDYYSAGAGKIMAKLAIERHAGRIMADVLWTSEVPDFYQLKGE
                     GLLERYVSPEASAVISPVSDPDGYFTAARLSTIGICYDITRINNPPRTWEDLARPEFR
                     DAFGIADPAISGTSMLSVSMIVKNLGWDYIRKLRANGAKMGQGATQVVDDTAVGDLAA
                     CLAVDYIAINKILDGAPLGFVYLDKIMVIPSPVAILKGTPNLKAARAFVDFLLSKEGQ
                     TIVAGGYTLPSRQDVPVARGVGLIPAEEAVKRAMCLDYIKLRSEKEEIIDRFTTIMTG
                     K"
     gene            5480..6475
                     /locus_tag="PLM74_06400"
     CDS             5480..6475
                     /locus_tag="PLM74_06400"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HON42746.1"
                     /translation="MKRALFVLCVLSVLLASAITVGAPKMKVMLYSSMKDSQLSALQK
                     AFSKKYPDIVFDYYTAGTGKVLTKLAAEQQAGGIAADMIWVGEPTNYVEFKQQGLLEK
                     YVSPEAKTINAIYKDKDGYYCGARIVGLGIAYNTNNVKGKDIPKDWSDLLNPKFKGFL
                     GMTDPAFSGTTLYTVAAFVQNPKFGYDFLKALKANDIKLVQGSSDSVTKVGSGEWDMS
                     IAVDYIAKDQMGQGSPVGFVYPAGGASIVASPIAIVKGTKNLEAAKILYDYILSLEGQ
                     KVLVEANTIPIRTEIKLPDVDLGKLLANALPVDDYKLVAEKEDMLDKFVEIMRKK"
     gene            6510..>6769
                     /locus_tag="PLM74_06405"
     CDS             6510..>6769
                     /locus_tag="PLM74_06405"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HON42747.1"
                     /translation="MAEVKLDRITVQYGEKTAIGDFSLHVRDGECLTLLGPSPCGKTT
                     VLRAIAGLAKLAGGEVTIGSRVVASKDRRIFIPPEKRNVGVV"
BASE COUNT         1437 a         1906 c         2024 g         1402 t
ORIGIN      
        1 catgatcggc agttacgctg tgaactcgag catgtgggat gtcggggtat gcctgggatt
       61 tggcgtgatt ggctggctac tgaagagata tggatactcg ttgcccccgc tggtactggg
      121 tatggtattg ggcggaatgg ccgaggtcaa cttgcgcagg gctacgatga tgttcgggcc
      181 aaccgccata ttccagcgat cgggcagcgt gattctgctc ctgatttcgg ccttgtcgtt
      241 cgccatccct gtgctgcagg ccagaagggc aaagacaaag gccagaacgt gaacccttga
      301 atgccgtgga accaaacagg aaaggggaca agggagatgg tcagaggcaa acaccgggca
      361 agcaggtggc ttggaacgct gattgccctt ctcgcactgg cgtcgctggc tccgaccgcg
      421 gccgaaacga ctatggtagc gccagacagc cgggagttga aactcctgat acagacggag
      481 gtctttgccg accgggccga cggatacgcg cggtgctggg gcattgtgaa ggacctggcg
      541 gctgaaacgg gcgtacaggc tacagaggcg aaggatccgt tcgcggaagg ggcccggaag
      601 gtgatgtacc tcgataccca ttctggcagg ctggaccaag agaaccgcag tcttcgccta
      661 aggatcaagc tgaaagacgg caagctagat gacaaggtag atctgaacct caagtaccgt
      721 aggagcgacg tggattctgt cccttctgat gcagtgacgg ctgcggcggg aatcaaggct
      781 tcgttctcct atgaggagga tgtcgccggc ttcattggcg gcgtagttgg gcagaatagc
      841 agcgccgctt cgatcgcttg cacggtcaaa ggagtcgccg cagacaagct tacaggcaac
      901 accctcgctg tgtacgctga gtacttcccc tcgctggccg tacttggcat tcctcttgat
      961 tcgccgcttg agatggtcgg aggcatcgcg atcagggaac acaaggtgac ccccggcgag
     1021 ctcgacttcg gtcagggcat gaagactgag gtagacatct cggtatggta caatttcgac
     1081 accggcgaga tcattacagc ggagatctcc tatggatccg cgttggggtc ggaggcgcct
     1141 gccgcggcgg ttgccaaggc tgaggcgttc ttcaacgccc tacaggagag aatggccggc
     1201 atcctcgctc cgggcggtat gaagacagac cttctccggt aaaccgccgg ggtcgcaaac
     1261 cgccggggcc gtaaaccgcc ggcagctcac ggaacgagcg ggagtgcttc atgcagagcg
     1321 tgcagtcttg actggacggc gccgcctggg atccatatca gtggattcct cccggcggcg
     1381 cttttccgcc tgtggggaga acccaggcgc acgttgtgca ttgcccccat cgctagagag
     1441 gacgcgagcc ttgcccactg gccgaacacc ctgcgccggc accttccttc gctcgttggc
     1501 ggcggttgtc ggcgaaagcg gccttacaca ggcatttagg caaaaccatg tttagcaggg
     1561 agttagctag cctgtaagaa ttgtgcccgg ttctccgccg atcctgctca gaatatgccc
     1621 ggtttggacg ggccggggac taagcccagc cgaattggga ctgtttagga atccaaatgc
     1681 ccgtctggag gcctttccgg gggcacgcga gagacgccca taggcgacca tattatgtaa
     1741 cccacctcta cctgcggatg tgcgaagggc atggctccca aaaaaggggt gctcccctgc
     1801 cggcgcgaat tctgacaatt accggcggcc agaccaggca ggatctgaac aggtggcccc
     1861 aaaagccggt tcagaaatga acaggatgcc tcagaacccc gttagggccc acgacaggag
     1921 gacacgaaga cccgatcatc ctggatcagc gaatgcacac ccagtttatc agggccgatc
     1981 aacgagcgga gttcagatgg aggcccacgg aggacacgcg gttttcaacg cctgctcata
     2041 cgcatcaaag gtatgctcat caaaacagac gaacctcact tcatcgatgg atggatctgt
     2101 ctgcagatag tcggtcactg ctctgatggc gatctgagcg gcctgcgata cgggataacc
     2161 gtacgcccca gtgctaatgg acggaaatgc cagcgtacga acaccgtgct ctgaggcaag
     2221 cttgagggag ttgatgtagc agtcggccag tagctccgct tcgcctgcat tgccgccctg
     2281 ccagattggg ccgaccgtgt gtatgacgta cctggcgggc agccggtatc ctcgggtaat
     2341 gacagcatcg ccggttgtgc aacctccgat tgtccggcat tccgcaagta ggtcagggcc
     2401 ggccgccagg tgtatcgctc cgtccacgcc gcctccgcca agcaaggtag tgttggcggc
     2461 gttgacgatt gcgtcaacac gctgctcagt aatatcgccg cgaaccacga agattccacc
     2521 gcggataccc ggcgcatcgt atcgctgctt accgtatctg ctcatgtcga tcacacctct
     2581 ttcggaatct gtagcggaat tcatcatcac ccgctgtgaa tcctgctgcg ctgagcggcg
     2641 cagagagctc cgcacagtag cttgctcgag cactcggctg gtagagagaa ggaaacgaga
     2701 gttcatgtat ggaatacaga gttcggagag aacccatggg agtgtctgca gatggggtga
     2761 cgcttttccc agctatgctc agtcgggtca ttgccgcggt tgatcgacgc cgactcgtgg
     2821 ctgttgtcct gttgctattg gcggtcatcc tgggtctcac cattctgagg cacacacgcg
     2881 tcccaagctg ggcaggcgat atggagactg actactgcac tctggtgata gatcaggctg
     2941 ctgccagcct taccgccttt ctcaaggact atgaggacgc aacctttcag tttgtggcca
     3001 atcaggacct caaccgtctg ctcctcgaat acgtctcagc cacagacact tacgaagtcg
     3061 ccccttacaa ccaggccttc tcaaatttcc tggaaggata tacgttcagc cgacgcggcg
     3121 tgtacgatgc ggtgttcgtt gacgagtcca acaggaatcg caaggcgttg accatgcgcg
     3181 agtcgctccc tgcgtcctac atcaaggctt tccgcaactc cggcacctac gaggagataa
     3241 tgcgtgccga cggcaaggca gtgtgggcat tcggcgaaga ctgtgatcgc gcgggcatga
     3301 attgcgtaat cgtgggcagg cgaataaagc acttgtatac acagaaaccg ttgggcattg
     3361 tggctatcat gatcgacgcc gacgaactgg cccgggtggt caacgactat ctgcatgatc
     3421 atttctactt ctcggtgggc acagtcaggg ccaattacac ggtcatagtg gatcgtgaag
     3481 ggtgcatcat ctccggaccc agtgctcctg ggccttcgcg tgacgagaaa ccttcttttc
     3541 tccgcgagct ctacccgaac ccacctgttg gccgggcaga ggggcgcttc acggggactg
     3601 tacgtggcga ggacgtgctg gtgatcttca ggcccatctc cgacactagc tggcgggcgt
     3661 tcgtgcctgt gtcgttggcc ggcctgtacg gtgaggagcc gggcggaggc gcttctgcgg
     3721 tgaggttgtc tattcccatg gccggcgggt tgatcgctgg cgctttggca gctaccgccg
     3781 tctttctttt cctttggccc agcccgcgca gtccgcgcag tccgcacggt ccatacagcc
     3841 cgcacattta ttcccccggg cgcaacggtg cttgcgtgga cgaggggcta gcgggcgcgc
     3901 gcggggccac tgaagctggc aggcttcagc ctgaagggcc tgatcccgag tggctcggcc
     3961 agctgactga caaggagata caggtcttga tcctgatgtc gcaggggtat tccaaccgcg
     4021 agatcgcgca gaagacctac atagcccaac agacggtcaa gaactacgtg agctgtatct
     4081 acagcaagct atgcgtgcgc gacagggtgc aggcgtcctt gctggcagtg gaggcgggcc
     4141 tgaatcttcg cgactatcgc gacctcgact gacgactgaa gagtgacaag tgacgagtga
     4201 cttgtcactt gccggtcatg atcgtggtga atctgtcgat tatctcttcc ttctccgacc
     4261 tcagcttgat gtagtcgagg cacattgctc tcttcactgc ttcttcagcg ggtatcaatc
     4321 ccactccgcg ggccacgggc acatcctggc gacttgggag agtgtagccg cccgccacga
     4381 tggtttggcc ctcttttgac aagaggaaat ccacgaaggc cctggcggcc ttgaggttgg
     4441 gcgtgccctt gagaatcgca acaggactcg gaatcaccat tatcttgtcg agatacacaa
     4501 agcccagggg cgctccgtca aggatcttgt tgatggcgat gtagtccacg gccaggcagg
     4561 ctgcgagatc gcctacggct gtgtcgtcca ctacttgagt agcgccttgt cccatcttgg
     4621 ccccattggc tctcagcttc cggatgtagt cccatcccag gttcttcacg atcatggata
     4681 cactcaacat cgaagtccct gaaatcgctg gatcggcgat gccaaaggca tctcggaact
     4741 cgggcctcgc caggtcctcc caggtccttg gagggttgtt tatacgcgtg atatcgtagc
     4801 atattccgat ggtgctgagg cgggcggcgg tgaagtagcc gtcgggatca gacacagggc
     4861 tgatcacggc ggacgcctcc ggcgatacgt acctttcgag tagaccttct cccttgagct
     4921 ggtagaagtc cggaacctcg ctggtccata gcacatcggc cattatcctt ccggcgtggc
     4981 gttcgatggc gagtttggcc atgatcttgc cggcgccggc tgagtagtag tcaagctgaa
     5041 tctcaggata cttgcgcatg aaagtatccc ttagctgccc gatgataaca tccttggtcg
     5101 atgtgtacac caccagccta gatttcggcg ccgcgtctgc ccaaggcgcc atcacaaccg
     5161 ccaacagcga tagcgcaagt agcaccgctc tgactgctgg ccgtctcttc attgatgacc
     5221 cctccttcac gcgctgccac gcggcatgcc aacgtaccta cccaagtaat atacaacagc
     5281 ctcctgccgt atcctcttcg tcgaactacg gggcgcctag ccatagcggt tcaccacggt
     5341 tcaccagcca tagtaccgtg gtactattcg cgcatctagc agcgaatggg gtaccgcggt
     5401 acggtgacgc cgctctatcc gcggcctatc atgtagtcta ggtggcgaat atcccaacta
     5461 aggtgaaagg gtgatactta tgaagagagc gctattcgtt ctgtgtgtgc tcagtgttct
     5521 gttggcatcg gccatcaccg tgggggcccc gaagatgaaa gtcatgttgt actcatccat
     5581 gaaggattct caactctcag cgttgcagaa ggccttctcg aagaaatacc ccgacatcgt
     5641 gttcgactac tacacagccg gcaccggcaa ggtcctgact aagcttgccg cggagcagca
     5701 ggcgggcggg atagcggccg acatgatctg ggtgggcgag cctacgaact acgtcgagtt
     5761 caagcagcag ggcctattgg agaagtatgt gtcgccggaa gccaagacca tcaacgccat
     5821 ctacaaagac aaggacggct attactgcgg ggcgagaata gtcggactgg gcatcgccta
     5881 caacaccaac aacgtcaagg gcaaggatat tcctaaggat tggagcgatc tgcttaaccc
     5941 taagttcaaa gggttcctgg ggatgactga ccctgccttc tccggcacca cgctctacac
     6001 tgttgcggcg ttcgtccaga atcccaagtt cggttacgac ttcttgaagg cgctgaaggc
     6061 caacgacatc aagctggtgc agggttccag cgactccgtg accaaagtcg gttcgggcga
     6121 atgggacatg agcatcgccg tcgactacat agccaaggac cagatgggtc aaggctctcc
     6181 agtgggcttt gtgtatcccg ccggcggcgc ctccatagtt gcaagcccca tagcaatagt
     6241 caagggcacg aagaacctcg aagcagcaaa gattctgtat gactacatcc tctcactgga
     6301 aggtcagaag gtcctagttg aggccaatac gatccccatt cgcacagaga taaagctgcc
     6361 agatgtcgac ctgggcaagc tgcttgccaa tgctctgcct gttgacgact acaagctggt
     6421 tgcagagaaa gaagacatgc tggacaagtt cgtcgagatc atgcggaaga agtagcatcg
     6481 gcgtccatca ccggcgaggg aggtttccca tggctgaagt caagttggac cggataacag
     6541 tgcagtacgg cgaaaagact gcgatcgggg acttctcgct tcacgtgcgg gatggagaat
     6601 gtcttacgct gctgggcccg tctccatgcg gcaagaccac cgtgctcagg gccatcgccg
     6661 gccttgcgaa gttggccggc ggtgaggtaa ccatcggcag cagggtcgtt gccagcaaag
     6721 acaggaggat cttcatccca ccggagaagc ggaacgtggg tgtggtgtt
//
LOCUS       DAOTAC010000121         6739 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_9636_length_6739_cov_4.154997, whole genome shotgun sequence.
ACCESSION   DAOTAC010000121 DAOTAC010000000
VERSION     DAOTAC010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6739
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9636_length_6739_cov_4.154997"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1631
                     /locus_tag="PLM74_06410"
     CDS             <1..1631
                     /locus_tag="PLM74_06410"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043163 - cell envelope organization
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HON42748.1"
                     /translation="ADLLVSGGGSLLQDVTGRRSVPYYAGIMIVARMLGVPVSVYAQG
                     VGPLNSGTSRWLARQALSRAQVVTLRDVGSMELVRQIGVTKPQPVLVADPAFALEPEC
                     TDGCEVSLPPRPLVMFALRPWPGPGAGDREVVYARVIQRVCTELGAHVTLLAFQPEVD
                     LDMAHRIVDLCEADGCVRPQIVALCGAPGAALSTIAHADLIVGMRLHSLIFAASAGVP
                     AVAIDYDPKVRAVAEQTGNVYVVPVDAGPEEIVRAVLDAWAHRDELRSELARRVPDLK
                     RQAREAARLALSSIVGRPAECGRARVLGVPVDCVSMDEAVEKAVEMATSRHGGHLVTL
                     NPEMTLSAGDDAALNDVIAGADLVVPDGIGVVRALDMLGYHPRGRVPGIELAANLMET
                     AAERGMSVYLVGAKPGVAQQAADAMSKAFPGLKISGTYHGYFAQTEEPAVLESIAQSG
                     AAFVFVGMGAGKQEKWIAHARNAAPGAVWIGVGGSFDVMSGNVRRAPAIYQRLGLEWL
                     YRLATEPRRAKRMSALPVFMFRVIGEACRGRSRS"
     gene            1650..2504
                     /locus_tag="PLM74_06415"
     CDS             1650..2504
                     /locus_tag="PLM74_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HON42749.1"
                     /translation="MERALLTTPEALRAKARDIRKHVVRMVGAASSGHPGGSLSAADI
                     VTVLYFNDMNIRPDDPGWPDRDRFVLSKGHAAPVLYAALAEAGYFPVDELITLRRIDS
                     HLQGHPSIRSTPGVEMSTGSLGQGLSAACGMAIAAKLDGATWRVYALLGDGELEEGQI
                     WEAVMTAAHRRLDNLTVFVDSNRLQIDGPVAEIKSMERIGERFAAFGWHVVDIDGHDI
                     GAIMAALAEAKRIKGRPTVVVAHTVKGKGVSFMENQVGWHGTAPKPDQVRAALAELDA
                     SGEGGDEK"
     gene            2501..3460
                     /locus_tag="PLM74_06420"
     CDS             2501..3460
                     /locus_tag="PLM74_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HON42750.1"
                     /translation="MTEMIATRAAYGEALARLGDVNPSVVVLDADLSASTRTAEFAKR
                     HPERFIQMGIAEQDMIGTAAGLAASGKIAFASTFAVFASGRAFDQVRNAVCYSRLNVK
                     ICATHAGLTVGEDGASHQSIEDIALMRAVPNMTVIVPSDAVQTAWAVNAAAALDGPVY
                     MRLGRAPVPVIYGEGQEFAIGKAQTVRAGNDVTIIACGIMVSRALAAAGALAEQGISA
                     RVLDMHTVKPLDIDAVVAAAAETGPIVTAEEHSIIGGLGGAVCEAVCESHPAPVVRVG
                     VRDQFGKSGKPDELLAAYGLTSDEIAEAARRAISMKSAAERIV"
     gene            3563..4249
                     /gene="ftsE"
                     /locus_tag="PLM74_06425"
     CDS             3563..4249
                     /gene="ftsE"
                     /locus_tag="PLM74_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018394591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HON42751.1"
                     /translation="MVKMYGVTRVYAGGVQALRGVDLSIDKGEFVFVVGPSGAGKSTL
                     LSMIYRGTVPTRGQVWVAGRNIARLRWHEVPLLRRRIGIVFQDFKLLPDRSVYDNVAF
                     ALEVIETSSREIAKRVPLALDMVGLAHKAQAMPDELAGGEQQRVAIARAIVNDPFIVI
                     ADEPTGNLDPDTSMDIMDLLCDINMRGTTVIMATHNQLVVDRLRRRVVQLNDGAVVRD
                     ELRGGYCGEA"
     gene            4239..5117
                     /gene="ftsX"
                     /locus_tag="PLM74_06430"
     CDS             4239..5117
                     /gene="ftsX"
                     /locus_tag="PLM74_06430"
                     /inference="COORDINATES: protein motif:HMM:NF038347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HON42752.1"
                     /translation="MKLRTMAMLGGQALRSLRKTPALTSIMVVMACMAAVSLFAVFAV
                     NLTAIGSQLQSQVQIRVFLDQNAPPDDKEALGRSIKALPGVTKVGYVPKDEALNRLRL
                     QMGESGFFLSDLEHNPLPDSFDVSLARAEDARAVADGIWKLKGVSDVTYGQKWVDNLV
                     SALRVLWGITGVVACLALIGASLIVSNTIRLSVFARRKEIEIMKLVGATDGFILFPFV
                     MEGLVIGIVGSFAAAGLVAAGYLGLVTWVESVASFIPIMKDPIHMGFATIGTVAFGAA
                     AGLAGSAISVRRYLKV"
     gene            5131..6339
                     /locus_tag="PLM74_06435"
     CDS             5131..6339
                     /locus_tag="PLM74_06435"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HON42753.1"
                     /translation="MKRLKALAALALVFVIAVGSTCGPAFASPDAGELSTKKNQLGDI
                     QTQIEKIRKTVSKYRTEEKNALVALQKTEDKLETLQANITATDRKLRNAEKQLSQAGA
                     DLSAAEQGLARATREYQASLSSLNNRLTELYKMGPGSYLELLLSSHDFADFITRYEFA
                     SMIVRQDAQTYLQLRAEKDSLEERRKDISERQAQLEKSRKEIGLLSANLDSEKKRLEQ
                     AMEDRAYYLQRVQAERARWEKELAEEERQSRELEKVIREMQARLGKSGQLAKWTGKFI
                     WPVVGGRISSPFGWRIHPIFRDRRFHSGIDIAIGTGTSVKAVADGYVLMAGWINGYGN
                     TVILDHGGGLSTLYAHNKSLSTSAGKAVLQGDIICKVGSTGFATGPHLHFEVRQDGSP
                     KDPISLLPAR"
     gene            6438..>6739
                     /locus_tag="PLM74_06440"
     CDS             6438..>6739
                     /locus_tag="PLM74_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42754.1"
                     /translation="MAEDRKGRGPGRLGWLAALGVLLALLVALAALAPTVDGVQSGGE
                     VAAPPKFRDAKTALEAMYLVKGEFYKPVPLSTLVRAYLSEGTVSGMLAVLGDPYTR"
BASE COUNT         1331 a         1770 c         2302 g         1336 t
ORIGIN      
        1 gggcggatct cctagtgagc ggcggaggaa gcctgctcca ggacgttacg ggccgccggt
       61 cggttcctta ctacgcgggc attatgattg tcgctcgtat gctgggcgtg cccgtgtcag
      121 tgtacgccca gggagtgggc ccgctcaact cgggcacgtc acggtggctt gcccggcagg
      181 cgctttcccg ggctcaagtg gtaaccctgc gcgacgttgg ctctatggag cttgttcgac
      241 agatcggggt gacgaagcca cagccggtac tggttgcgga tccggcgttt gcgctggagc
      301 cagagtgcac ggatggttgt gaggtctcgc tgcctccccg tcctcttgtg atgtttgcgc
      361 tcaggccgtg gcccgggccc ggtgcgggag accgtgaggt cgtgtatgcc cgtgtgatcc
      421 agagggtttg caccgagctt ggagcccatg tcacgctgct ggcttttcag cctgaggttg
      481 acctcgatat ggcccaccgc attgtggact tgtgtgaagc ggacggctgt gtgcgtccgc
      541 agatcgtcgc gctctgcggt gctccggggg ctgcgctgtc taccatagcc catgcagacc
      601 tgatcgtagg catgaggctt cattcgctga tattcgcggc atctgcgggc gttccggcag
      661 tggcaatcga ttacgacccc aaggtgcggg ccgtggctga acaaacgggc aacgtctatg
      721 tagtaccggt tgacgctggg cccgaagaga ttgtccgcgc cgtgctggat gcctgggcgc
      781 accgtgacga gctccgctcc gagctggcga ggcgagtgcc tgacctgaaa agacaggcac
      841 gcgaggcggc caggcttgcg ctgtcgtcta tcgttggcag gccggcagaa tgcgggcggg
      901 cgcgcgtgct cggcgtgccg gtggactgcg tgagcatgga cgaggccgtg gagaaggcgg
      961 ttgagatggc tacttcgcgt catggcggtc atttggtgac tctcaacccc gagatgacct
     1021 tgtcagccgg ggacgatgct gctctgaatg acgtgatagc aggtgcggac ctggtagtcc
     1081 cagacggaat cggggtggtc cgcgccctcg atatgcttgg ataccacccg cgagggcggg
     1141 tgcccggtat tgagctcgcc gccaacttga tggagacagc tgcggagcgg ggaatgtcag
     1201 tctatctggt aggcgcaaag ccgggtgtgg cgcagcaggc tgcagatgca atgtcgaagg
     1261 cctttccggg ccttaagatc agcggcactt atcatgggta cttcgctcaa accgaggaac
     1321 cggctgttct cgagagcatt gcccaatccg gcgctgcgtt cgttttcgtg gggatgggtg
     1381 ccggcaaaca ggaaaaatgg atagctcatg cgcgtaacgc ggcgccgggc gcggtatgga
     1441 taggcgtggg gggctcgttc gatgtgatgt cgggcaatgt gaggcgggct cctgcgatct
     1501 atcagagact ggggctggag tggctttatc ggctggcgac cgaaccgcgg agagcgaaac
     1561 ggatgtcggc gcttcccgtg ttcatgttca gagtgattgg ggaggcatgt cgaggacgga
     1621 gcaggagttg aggaaggagc tggtagttga tggaacgcgc gttacttact acccctgagg
     1681 cgcttagagc caaggcgcgc gacatcagga agcacgtagt cagaatggtg ggcgcggcat
     1741 cgtcagggca tcctggcggg tcgctgtcgg ccgccgatat tgtgacagtg ctgtatttca
     1801 acgatatgaa catcaggccg gacgatcccg ggtggcctga ccgtgatcgt tttgtgcttt
     1861 caaaggggca cgcggcgccg gtgctctatg cggcgctggc ggaggcgggg tatttccccg
     1921 tcgatgagct gatcactctg cggcgaatcg acagccatct tcagggccat cctagcatcc
     1981 gcagcacacc cggggtggag atgtccaccg gctcgctggg ccagggcctg tcggcggcat
     2041 gtggaatggc gatagcggcc aagctggatg gggctacgtg gcgcgtgtac gccctgctgg
     2101 gcgatggcga attggaagag ggccagatct gggaggctgt gatgaccgcc gcccaccgca
     2161 ggctcgacaa cctcacggtc tttgttgact ccaatcgcct ccagatagat gggccggttg
     2221 ccgagatcaa gtccatggag cgaattggag agaggtttgc cgccttcgga tggcatgtcg
     2281 tggatataga cgggcacgac atcggggcca tcatggccgc gctggccgaa gccaagcgta
     2341 tcaagggcag gcccacagtg gtagtcgccc acacggttaa ggggaaggga gtctcattca
     2401 tggagaatca ggtggggtgg cacggcactg ctcccaaacc ggatcaggtc cgggcggccc
     2461 tggctgagct ggacgcatct ggcgaaggcg gtgacgagaa gtgactgaga tgattgccac
     2521 cagggcggcc tatggcgagg ctctggcgcg cctcggagac gtgaacccca gtgtagtagt
     2581 gttggatgca gacctgtcgg cgtctaccag gacggctgag tttgccaaga gacatccgga
     2641 gcggtttatc cagatgggca tagccgagca ggacatgatc ggcacagcag ccggactggc
     2701 tgcatcaggc aagatcgcat tcgccagcac ctttgcggtg ttcgcaagcg gccgagcgtt
     2761 cgatcaggtg cggaacgcgg tatgttactc aaggctgaat gtgaagatat gcgcaaccca
     2821 cgccggcctg acagtgggcg aagatggcgc ttcgcaccaa tcgatagaag acatcgccct
     2881 gatgagggcg gtgcccaaca tgacggtgat cgtaccgtca gacgcggttc agacggcatg
     2941 ggctgtgaac gctgcggccg cgctggacgg tccggtctac atgagactgg gcagggcgcc
     3001 tgtgccggtg atctatgggg aggggcagga gttcgccata ggcaaggccc aaacggtgcg
     3061 cgcagggaat gacgtgacca taatcgcgtg cggcatcatg gtatcgcggg cgctggctgc
     3121 cgccggagcc ctggcagagc agggaatcag cgccagggtg ctagacatgc atactgtcaa
     3181 gcctttggac atcgatgccg tggtggccgc agccgctgag accgggccca tcgtcacggc
     3241 agaggagcac tccatcatcg gcgggctggg cggagcggtg tgtgaagctg tgtgtgagtc
     3301 gcacccagcg cctgtcgtgc gagtgggagt gcgcgatcag ttcggaaagt cgggcaagcc
     3361 cgatgagctg ctcgcagcgt atggtctcac cagtgacgag atcgcagagg cggcgaggcg
     3421 ggccatatct atgaagagtg cggcggagag gatcgtctga ggcacatatc ccctcgattt
     3481 ccatcccgtc tcagcaggaa tcggcggcag ccgagaagaa catctgaatg gtgtgacctg
     3541 cttgagacgg ggtgatcttg ctttggtcaa gatgtacgga gtcacgaggg tgtacgccgg
     3601 cggcgtgcag gcgctgcgag gcgtggacct ttcaatcgat aagggcgaat tcgtttttgt
     3661 ggtggggccc agtggcgcgg gcaaatctac gctcctgtcc atgatatacc gaggcacagt
     3721 ccccacacgc ggccaggtgt gggtggccgg gcggaacatt gccagactgc gatggcacga
     3781 agtgccactt cttcggcgcc ggatcggcat tgtgtttcaa gacttcaagc tactgcctga
     3841 tcggtcagtg tacgacaacg tcgcattcgc cctggaagtg atagaaacct cttcccgcga
     3901 gattgccaag cgggttccgc ttgccctgga tatggtgggc ctggcgcaca aagcgcaggc
     3961 catgccggat gagctcgccg ggggggaaca acagcgggtg gcgattgcac gtgcgatcgt
     4021 aaacgatcca ttcatagtga tcgcagacga gcccaccggc aatctggatc ccgacacttc
     4081 catggatatc atggacctcc tgtgcgatat caatatgcgc ggcactacag tgattatggc
     4141 tactcacaac cagctcgtcg tggatcggct gaggcggcgc gtagtgcagc tgaacgatgg
     4201 tgcagttgtc cgggatgagc tgcgaggtgg atactgcggt gaagcttagg acgatggcga
     4261 tgctgggagg gcaggcactg cgcagcctca ggaagacacc ggccctcacg tcgatcatgg
     4321 tagtaatggc atgtatggcg gctgtaagcc ttttcgctgt gttcgccgtg aacctaacgg
     4381 ccatcggcag ccagctgcag tcgcaggtac agatcagggt ttttctcgat cagaacgccc
     4441 ccccagatga caaagaggcg ctcggccgtt ccataaaggc ccttcccgga gtcactaagg
     4501 ttgggtatgt ccctaaggac gaagcgctca accgtcttcg gctgcagatg ggagagagcg
     4561 ggttcttcct gagcgatctg gagcacaacc cgcttccgga ttcgtttgac gtgtcgcttg
     4621 cgcgtgctga ggacgccagg gcagtggctg acggcatatg gaaactcaag ggggtttccg
     4681 acgtcacgta cgggcagaag tgggttgata acctcgtcag cgctcttcgg gtactgtggg
     4741 gcatcacggg ggttgtggca tgcctggcgc tgattggggc tagccttata gtgtccaaca
     4801 cgatcagact gtccgtattc gccaggcgca aagagattga gatcatgaag cttgtcgggg
     4861 caacagatgg gttcatcctc tttccgttcg tcatggaagg cctggtcatt ggaatagtcg
     4921 ggtctttcgc ggcagcgggt ttagttgccg caggctactt gggccttgtg acctgggtag
     4981 agtcggtggc atcattcatt cccatcatga aggatccgat ccatatggga ttcgcgacca
     5041 tagggacggt ggcgtttggg gccgccgccg ggctggccgg aagcgccatt tcggtgaggc
     5101 gatatctgaa ggtgtgagag ggtgtggcgt atgaagcggc tgaaggcgct ggcggcattg
     5161 gctttggtgt ttgtgatcgc cgttggctct acctgcggcc cggcattcgc gagcccggac
     5221 gctggcgagt tgtcgaccaa gaagaaccag ctcggtgata tccagacaca gatagagaag
     5281 atccgcaaaa ctgtgtcaaa gtatcgaaca gaggagaaaa acgctctggt cgcgctgcag
     5341 aagaccgaag acaagctgga gacgcttcag gcgaacatca ctgcaacaga ccgaaaactg
     5401 cgaaatgccg agaaacaact gtcacaggca ggtgccgatt tgagcgccgc tgagcaaggg
     5461 ctggccaggg cgactcgtga gtaccaggct tcactgagta gcctgaacaa ccgcctgact
     5521 gagctttaca agatgggtcc tggctcttac cttgaactgc tgctcagttc gcatgatttc
     5581 gccgatttca tcaccagata tgagtttgcc agcatgatcg tgcggcagga cgcccaaacc
     5641 tatctgcagc tcagagcaga gaaggactcg ctcgaggagc ggaggaagga catctccgag
     5701 cgtcaagcgc agctggagaa gtcgaggaag gagatcggtc tgctttcagc caaccttgac
     5761 agcgagaaga aacgattgga gcaggccatg gaagatcggg cctactatct ccaacgtgtg
     5821 caggctgaga gggcgcgttg ggagaaggaa ctcgcggaag aagaacggca atcacgcgag
     5881 ctggagaaag tcatacggga aatgcaggca cgactcggga aatccggcca gctcgccaag
     5941 tggacgggca agttcatctg gcctgtggtc ggcggtcgga tctcttctcc ttttggctgg
     6001 cgcatccatc cgatcttccg cgacagacgt tttcactcag gtatcgacat cgctatcggc
     6061 acgggcacat cggtcaaggc agtcgcagac ggatacgtgt tgatggcggg ctggatcaac
     6121 ggctacggca acaccgtcat actcgaccac ggcgggggat tatccacgct gtatgcgcac
     6181 aacaaatctc tgtccacaag cgctggcaaa gcggtgctcc aaggtgatat catatgtaaa
     6241 gtggggtcta ccggtttcgc gacaggaccc catcttcact ttgaggtgag gcaggatggc
     6301 tcgcccaaag accccatcag cctgctgccg gcgcgttagc tccgttgatc gggcaagtgc
     6361 atgcggctac ccggggcggg catgggacat gacgtttggc ataggatgta cggaatgccg
     6421 aaagtgagtg tgtgactatg gctgaagata gaaaaggacg aggtccgggc aggcttggtt
     6481 ggcttgcagc gttgggcgtg ctgctcgcgc tgctggtagc gctcgcggct ttagcgccca
     6541 cggttgatgg agttcagtcg ggaggcgagg tcgcagctcc gcccaagttt agggatgcca
     6601 aaaccgccct ggaggccatg tacctggtta agggagagtt ctacaagccg gtgcccttgt
     6661 cgacactggt ccgggcttac ctatctgagg gcactgtatc aggtatgctg gcggtcctgg
     6721 gcgacccata cacccggta
//
LOCUS       DAOTAC010000122         6709 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_9692_length_6709_cov_4.564022, whole genome shotgun sequence.
ACCESSION   DAOTAC010000122 DAOTAC010000000
VERSION     DAOTAC010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6709
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9692_length_6709_cov_4.564022"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /locus_tag="PLM74_06445"
     CDS             <1..862
                     /locus_tag="PLM74_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HON42755.1"
                     /translation="LSAVGIAKSLGNVRLTGLHCHIGSQVFEMEPFVDAVDIMMDFAR
                     QAHEATGFVMEELDIGGGLGIRYNSKDDPITARDLADVVAPALIDAASRHGLPMPSLL
                     LEPGRSIVGEAGTTLYTVNAVKRIDGVRTYVSVDGGMADNPRVALYQAEYEAALANRM
                     AVPGQASPESPESWETVSVAGNCCESGDMLVWDVAMPRPEVGDTLAVFCTGAYNYSMA
                     SNYNRYPRPAVVLVRDGRADVIVERETYSDLVAHDLVPSRLRPAAAPPAVHPAAAESA
                     HRDSAGARSR"
     gene            1245..1466
                     /locus_tag="PLM74_06450"
     CDS             1245..1466
                     /locus_tag="PLM74_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HON42756.1"
                     /translation="MREKVEAVIGKIRPSLQADGGDVELVDVTADGIVKVRLKGACAG
                     CPMSTMTLKNGIERILKQEISEVRSVQAV"
     gene            complement(1604..2818)
                     /locus_tag="PLM74_06455"
     CDS             complement(1604..2818)
                     /locus_tag="PLM74_06455"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HON42757.1"
                     /translation="MNVKRGPSKQLIARLAGLILIGLVMAIVFSRGYDDMAARRGEPT
                     GQGDGFRIVELPPEPESMPEPVPKHDEDIATAQANPQTSYGESPRSLWAGGQEGQEPL
                     PRGSQSADMCSCGDLHRLWLEEPRMVGEHVQDLQEGLAMAGYYSGPADGVFGTGTDRA
                     VRAFQGSVGLPQNGVVDMAVWMALGLACEPIMAASLKPSGDIIAASPGKGQAPWRAIL
                     IDTSNLTLTLLENGKPVSQYRVGVGKSSTPTPLGYFIVTEKAAWAGGFGTRWMGLSVP
                     WGKYGIHGTNKPWTVGQRKSGGCVRMLNRDVEKLYALVEVGMPVVITAGHFGVLGSTR
                     PRVEPGAKGSYVYAIQLRLAKLGYEHAGVDGVYGQATERAIMRLQRDKGLPVTGIVDM
                     QTYEALGMSVMD"
     gene            3068..4108
                     /gene="tdh"
                     /locus_tag="PLM74_06460"
     CDS             3068..4108
                     /gene="tdh"
                     /locus_tag="PLM74_06460"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006569608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HON42758.1"
                     /translation="MRGEMRAIAKTSPSPGAEKVVVPIPQPGRGEVLIKVRATAICGT
                     DVHIYEWDQWSQGRVKTPRIFGHEFCGDVVEVGEGVTKVQVGQFVSAETHTACGHCFY
                     CLTGQAHICREAKIVGIDRDGCFAEYVVLPEFNCWIWDIDIPDEVAAIQDPFGNAVHT
                     ALATDLAAKTVLITGMGPIGLCAISVAKRSGATEVYVTDVSEYRLDLAKKLGADLAIN
                     PIEEDPVAAVREATGGLGADVLLEMSGNPTAIRQGFQALRKAGFASLLGIPSKPMEID
                     LANDIIFRSLTVQGINGRRMFDTWYQARALLKAGIDITPVITHRMSLWDDLDKGMELM
                     MSGKCGKVILYP"
     gene            4156..5346
                     /locus_tag="PLM74_06465"
     CDS             4156..5346
                     /locus_tag="PLM74_06465"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HON42759.1"
                     /translation="MSDALAYLADELAELREQKLFRYPKVLRGQQQARAIYDGREVIN
                     LSSNNYLGLATHPRMIAAAAKAAQEFGAGSGAVRTIAGTMDMHVELEKKIAAFKHTEA
                     SLVFQSGFTANAGTVAAVLGKDDVIVSDELNHASIIDGCRLSRAAIKVFPHGDIDALK
                     QLLDETDAVRDAGHHVLVVTDGVFSMDGDIAKLPEIVRAAKEHGAITMVDDAHASGVL
                     GRNGRGTVDHFDLHGQVDIQVGTLSKAIGVLGGYVAGSRTLIDYLIHRGRPILFSTSH
                     PPAVTAACLEAFDILVEEPEIIDKLWENTRFFKKGLEDLGFNTGISQTPITPVIVGEG
                     ALAMKLSDRLFEEGVFAQALAFPTVPRGKARVRTIVMATHTQEDLGIALKAFEKVGKE
                     LSII"
     gene            5403..6332
                     /locus_tag="PLM74_06470"
     CDS             5403..6332
                     /locus_tag="PLM74_06470"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HON42760.1"
                     /translation="MPEVVYAEETLKTQDGIDLFCRVWLPGGPSGATDAGGDAGAGTA
                     AAAASAATRAGRVRAVVVICHGQAEHSGRYTHVAQFFAERGYAVYAYDLRGQGKSGGV
                     RGHVDRWTQYYDDLWLVIRTAKGRHSGLKLFVIGHSLGGLIALGYAERRPKTITGVVV
                     SSPGLAFMVKVPLWKKALATVLSRIVPRTAFETGIQAEQLSHDPAVAAAYKSDKIRYP
                     KVTARLYVEWNRATRAVFEGAKSFAVPCLFMPAGADQICSAQATVDFYNNDPHPDKKL
                     VVWEGLYHEIFNEPEKQKVLETAAAWIAERDGR"
     gene            6368..6493
                     /locus_tag="PLM74_06475"
                     /pseudo
     CDS             6368..6493
                     /locus_tag="PLM74_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831915.1"
                     /note="The proteins in this cluster have high sequence
                     similarity to MoeB and are possibly involved in the
                     synthesis of molybdopterin, but there has been no
                     biochemical or physiological characterization. There is
                     also no genetic linkage to other molybdopterin cofactor
                     synthesis proteins. These proteins are similar to a
                     Pseudomonas stutzeri protein which is essential to
                     pyridine-2,6-bis(thiocarboxylic acid) synthesis that
                     possibly activates a substrate by adenylation; incomplete;
                     partial in the middle of a contig; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/sulfurtransferase MoeZ"
BASE COUNT         1353 a         1856 c         2162 g         1338 t
ORIGIN      
        1 cctgagcgct gtgggcatcg ccaaaagcct gggaaacgta cggctgaccg ggctacactg
       61 ccacataggc tcacaggtgt tcgaaatgga gccttttgtc gacgcggtcg acatcatgat
      121 ggacttcgcc aggcaggccc atgaggctac cgggtttgtc atggaagagc ttgatatcgg
      181 cggcgggctg ggaatccgct acaattcgaa ggacgatccc attacggcac gtgatcttgc
      241 cgatgtcgtt gccccggcgc tgatcgacgc cgcgtccagg catggactgc ccatgccgtc
      301 actgctgctg gagccgggaa ggtcaatcgt gggcgaggcc ggcactacgc tgtacacggt
      361 gaacgccgtt aagcgtattg acggtgtcag gacatacgtt tcggtggacg gaggcatggc
      421 ggataatccg cgcgtggcgc tttaccaggc cgaatacgaa gcggcgttgg caaaccggat
      481 ggcggttccc ggccaggcga gcccggagag ccccgaaagt tgggagaccg tttcggttgc
      541 cgggaattgc tgcgaatccg gcgacatgct tgtatgggat gtcgccatgc cgcgacctga
      601 agtcggcgac acgctcgccg tgttctgcac aggtgcctac aactattcca tggcgagcaa
      661 ctataaccgt tacccgcgac ctgcggtcgt gcttgttcgc gatggccggg ccgatgtgat
      721 cgtggaacgg gagacatatt ctgatcttgt agcgcacgat ttggttccaa gcaggctgag
      781 acctgccgcg gcacctcctg cggtacaccc tgcagctgcg gagtcggctc atcgcgactc
      841 agccggcgct cggtctcgct gaaatcccgg tatcctgatc catgttttcg ttgctttacg
      901 tagtacggta gtcaaacctc tccttctgca caagcgagca atgcgcgaat gaacaccttg
      961 gccgagcctg cccacatatc gtacagtcgg cctggcagtg ggacttcccc tccaaggagg
     1021 ccttacgcgg ggcgtaagct acgaaagcgg tgctgagcag ggattggcac aaccgtatgg
     1081 aaaaccatac agtcgccaag caggcgcaga agtgttccat cttggcaggg gcgcaggatt
     1141 gtgaactccg tgccgacgac agtcttggtg aagcaggcgt gttgttgacc ctcattgggt
     1201 ggaatgatag tattgtggca cgtagcggaa aggggctgag cggcatgcgt gagaaggttg
     1261 aggctgttat cggcaagata cggccatcgc tgcaggctga cggcggcgat gtcgaacttg
     1321 tggatgtgac tgccgacgga atcgtgaaag tcaggctgaa gggggcctgt gcaggctgcc
     1381 cgatgtcgac gatgacgctt aagaatggga ttgagaggat acttaagcag gagatctccg
     1441 aggtaagatc ggttcaggcg gtttaggtat cagcctggat aatcgggtaa cgcgggggcc
     1501 atgatcctca cgactcgagt aggaggatgg atggcctcct tatcgattcg gcgaccaggg
     1561 atgccccgga acctcgttcg ggctcaccac ccgtgatcgt tgtctagtcc attaccgaca
     1621 ttcccagagc ttcgtatgtt tgcatatcga caatacctgt cacaggcagt cccttgtctc
     1681 gctgcaatcg catgattgcg cgctcggttg cctgcccgta tacgccgtct acacctgcat
     1741 gctcgtaccc caacttcgca agtctcaact gtatcgcgta cacatagcta cccttggcgc
     1801 ccggctctac ccgggggcgg gtcgatccta gcacgccgaa gtgcccggca gttatgacta
     1861 ccggcatgcc tacctcaact aaggcataga gcttctcaac atcgcggttg agcatgcgta
     1921 cgcacccgcc ggacttgcgt tgcccgacag tccacggttt gttggttccg tggatgccgt
     1981 acttacccca gggcacgctc aagcccatcc acctagtgcc gaacccgcct gcccatgcgg
     2041 ccttctcggt cactatgaag tagcccagag gagtgggcgt agatgatttg ccgaccccca
     2101 cgcggtactg gctcacgggt ttcccgttct cgagaagggt cagtgtgagg ttggacgtat
     2161 ctatgagaat ggcacgccag ggcgcctggc cttttccggg actggctgcg ataatatcgc
     2221 cgctgggctt aagcgaggcg gccataatag gctcacatgc aagcccaaga gccatccata
     2281 ccgccatgtc gacaacgccg ttctggggca gtccgacgct accctgaaaa gcccgcactg
     2341 cgcggtcggt tccggtcccg aaaacgccgt ctgcagggcc gctgtagtac cccgccatcg
     2401 caagcccttc ttgcaggtct tgcacatgct ccccgaccat cctcggctct tccagccaga
     2461 ggcggtgcag atcgccgcat gagcacatgt ctgcgctctg cgaacctcgc ggcagcggtt
     2521 cttgtccttc ctgaccgccc gcccatagcg aacggggcga ttctccgtag ctcgtctggg
     2581 ggttcgcctg ggctgtggcg atgtcttcat catgcttggg cacaggttcg ggcatagact
     2641 cgggctccgg cgggagctcg acgatgcgga acccgtcacc ctgccctgtt ggttcgccgc
     2701 gcctggcagc catgtcgtca tatccgcgcg agaatacgat cgccatgacc aacccgatca
     2761 gtatgagccc ggccaggcgg gctatgagct gcttgctagg gcctcgcttc acattcatca
     2821 cccatgcccc gagatctctc cattgatgca tttcccagat aatccatcca gcatagtgta
     2881 tccagcaact caatggttaa cgcggcatgt cccgcagcgc gtccgcagca cacatcgcgt
     2941 gacaaccagg gagccttcga cgtgatgtga agtcaagaag gtatggacgc acgcgactag
     3001 aatagatatt acggtacctt agaatggccg atcggcacag gttggccttg ttgaaggggg
     3061 cttacggatg cggggagaga tgcgggccat agccaagaca tcgccgtctc caggcgccga
     3121 aaaggtagtg gttcccattc cccagcccgg caggggcgag gtattgatca aagtgcgcgc
     3181 gactgccatc tgcggcaccg acgtgcacat ttacgagtgg gatcagtggt ctcaaggcag
     3241 ggtgaagacg ccgaggatct ttgggcacga gttctgcgga gatgtcgtcg aggtgggcga
     3301 aggcgtcacc aaagtccagg taggtcagtt tgtatcggcg gagacgcaca ctgcgtgcgg
     3361 gcattgcttc tactgcctga ccgggcaggc gcacatctgt cgagaggcca agattgtggg
     3421 catcgacaga gatgggtgct tcgccgagta cgtcgtcctg cctgagttca actgctggat
     3481 atgggacatc gacatacctg atgaagtcgc ggccatacag gatccgtttg gcaatgcggt
     3541 ccatacggcg cttgcaacgg acctggccgc gaagactgtc ctcataacag gtatgggccc
     3601 cattggcctc tgtgctatca gtgttgcgaa gcgctcgggc gccacggaag tgtatgtcac
     3661 agatgtgtcg gagtaccggc ttgatctggc aaagaaactc ggcgcagacc tggcgataaa
     3721 cccgattgag gaagaccccg tcgcggctgt gagggaagcc acgggagggc ttggagccga
     3781 tgtactgctg gagatgtcgg gcaatcctac tgccatacga caagggtttc aggcgctgcg
     3841 caaggcgggg ttcgcatcgc ttctaggcat tccgtcgaag ccgatggaga tagacctcgc
     3901 gaacgatatc atattccgaa gcctcactgt gcagggcatc aacgggcgca ggatgtttga
     3961 cacttggtac caggcccggg cgctgcttaa ggcggggatc gacataacgc cggtcatcac
     4021 gcacaggatg tcgctatggg atgatctaga caagggcatg gagcttatga tgtcgggaaa
     4081 atgcggaaag gtaatcctgt acccgtaagg cagcgaggtg ttttcacgca tatttcgatg
     4141 ggggaggcat tgcatatgtc tgacgcgttg gcatatctcg cggacgagct tgccgagcta
     4201 cgcgagcaga agctgttcag gtaccctaag gtcttgaggg ggcagcaaca agccagggcc
     4261 atatacgatg gccgcgaggt catcaacctc tcgtccaaca actacctggg cctcgccaca
     4321 cacccgcgga tgatcgcagc tgcggcaaag gcggcgcagg agttcggagc gggctcaggc
     4381 gccgttcgta ctatagcggg cacaatggac atgcacgtcg agttggagaa gaagatagcc
     4441 gcgttcaaac atactgaagc gtcactggtg tttcagtctg ggttcacagc caatgcgggc
     4501 acggtagccg cggttctggg caaggatgac gtcatcgtca gcgatgagct gaaccacgcc
     4561 agcatcatag acgggtgcag gctgtcgcgg gccgccatca aggtcttccc acatggcgat
     4621 atcgacgccc tgaagcagct gctcgacgag actgacgctg tgcgtgatgc gggccatcat
     4681 gttctggtcg tgactgacgg cgtgttcagc atggatggtg acatcgcaaa gcttccggag
     4741 atcgttcggg cggccaaaga gcacggcgcg atcaccatgg tggacgatgc ccacgccagc
     4801 ggtgtgctgg ggcggaacgg tcggggaacg gtcgaccact tcgacctgca cggccaggta
     4861 gatatccaag tgggcacgtt gtcgaaggcc atcggcgtgc tcggcggata cgtggcgggc
     4921 tccaggacgc tgatcgacta cttgatccat cgtgggcgac ctatcttgtt cagcacgtca
     4981 catccgccgg cggttacggc tgcgtgcctc gaggcattcg atatcctggt ggaggagccg
     5041 gagatcatcg acaagctgtg ggagaacacc cggttcttca agaaggggct tgaggactta
     5101 gggttcaaca ctggcatcag ccagaccccc atcactccgg tcattgtagg cgagggcgcg
     5161 ctggcgatga agttgtccga caggctcttc gaggaaggtg tgtttgctca ggctctggca
     5221 ttcccgaccg tgccgcgggg caaggccagg gtacgcacga tcgttatggc cacgcacacg
     5281 caggaagatc tgggcatcgc gcttaaggcc ttcgaaaagg taggcaagga actgagcatc
     5341 atctgaggct gctgggtggc ggggtccgct ggcccgaccg ggcgccaaag gaggggcgga
     5401 tattgccaga agtcgtctat gctgaagaga ccctcaagac tcaggatgga atcgatctgt
     5461 tctgccgggt gtggctgccg ggtgggccca gcggtgcgac tgatgccgga ggggacgcgg
     5521 gagccgggac ggctgccgct gcggcttctg ctgctacgcg cgcgggtcga gtgagggctg
     5581 tcgtggtgat ctgccacggt caagctgagc actccggaag atacacgcat gtggcgcaat
     5641 tcttcgcaga gcggggatac gcggtgtacg cctatgacct tcgcgggcag ggcaagtccg
     5701 gcggtgtgcg cggccatgtg gaccgatgga cgcagtacta cgacgatcta tggcttgtga
     5761 tccgcaccgc caaggggcgc cactccgggc ttaagctgtt tgttatcggg cattccttgg
     5821 gaggcctcat cgctctaggg tacgccgagc ggcggcccaa gacaatcacc ggtgtcgttg
     5881 tcagctcgcc aggccttgcc tttatggtga aagtgccgct ctggaagaag gcactcgcaa
     5941 cggtactctc gcggattgtg cctcgcacgg catttgaaac cggcattcag gccgagcaac
     6001 tgtcgcatga ccccgcggtt gcggcagcat acaagtctga caagatcaga tacccgaagg
     6061 tgactgccag gctctacgtc gagtggaatc gggccacgcg cgcggtcttc gagggcgcga
     6121 agtcgttcgc ggtaccatgc ttgttcatgc cggccggcgc cgatcagatc tgctctgcgc
     6181 aggctaccgt ggacttctac aacaacgacc cacaccctga caagaagttg gttgtgtggg
     6241 agggcctgta tcacgagatc ttcaacgagc cggagaagca gaaggtcttg gagactgccg
     6301 cggcctggat tgccgagcgt gatggacggt agggggatag gggcgatagg ctgaacaggc
     6361 actcgagctg acagctgagg atatccagcg atatagcagg catctgtgcc tcgcaggtgt
     6421 tggcgaggaa ggtcagagga gaattcggtc ggccagggcc ctggttatga gggtgggagg
     6481 actgggttct cctattgcgc tcagggcctt cgagaaggta ggcaaggaat tgagcatcat
     6541 ctgaggctgc tgggcgacgg ggcttgccgg cccggccggg cgacaaagga gggactgata
     6601 ttgccagagg tcgtctattc cgaagagact ctcaaaaccc aggatggaat cgagttgttc
     6661 tgtcgggtat ggttgccggg cgggcccagc ggggcaagtg attccggag
//
LOCUS       DAOTAC010000123         6702 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_9699_length_6702_cov_4.763352, whole genome shotgun sequence.
ACCESSION   DAOTAC010000123 DAOTAC010000000
VERSION     DAOTAC010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6702
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9699_length_6702_cov_4.763352"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="PLM74_06480"
     CDS             <1..489
                     /locus_tag="PLM74_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42761.1"
                     /translation="GEFALEFEGFGEEPAEESEPVSSSDLDLYLSDQPEEPEVGEPAT
                     TRKTIEMEYEFSKDSVIERIVDSGGLPHRFSQLEVLRAPIKKGKTWSFQTPDWTDVKA
                     EIIETGKDAHKADYVRVRYEAEVDGGSYIETRLFKKGLGMVEFTSSGPDGAEFVYSLI
                     GN"
     gene            complement(694..2424)
                     /locus_tag="PLM74_06485"
     CDS             complement(694..2424)
                     /locus_tag="PLM74_06485"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HON42762.1"
                     /translation="MDYIAVFERWRDAAAMLDESSRQELDRISFDAREIEERFGSELA
                     FGTGGMRGKLGVGTARMNRYTVRCATHGYAAYLLARVPGAREAGVVIARDPRRGSTEF
                     ADETARVLASHGIRVWLFDDIRPTPVLSYAVRELAAAGGIVITASHNPSEYNGYKAYD
                     AHGVQLLPDEAGEVADRARAVDDYLSPPGEPKAPVEYVDAAVDESYVRRVLDLVNGIA
                     GALAPREARASFGLIYTPLHGTGHVFIPRVLRELGFASAQTVAEQSVPDGAFPTVRYP
                     NPEDPGAFEMAIAQAHDAGGALPGIILATDPDCDRVGVAVWDQAAERYRVLTGNQTGV
                     LLADLLIEGARASDRDLAGYAIIKTIVSTEMVTAIAAASGVRVIDTLTGFKYIGEKIG
                     EMERAGDRFLLGFEESCGYLAGTFVRDKDAVIASALIACAAVRQRLRGKTLLERLDEL
                     FHEYGHFHERLLSVPIAASVGAGEAMESLRRDTPDSVGGFPVVEVHDYLTGWTKRRGT
                     GVRVPIDLPRENCIQLKLTDLGKVTLRPSGTEPKLKLYIAVRADSADEALARADAIES
                     ATQRLLPIAP"
     gene            2992..3426
                     /locus_tag="PLM74_06490"
     CDS             2992..3426
                     /locus_tag="PLM74_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42763.1"
                     /translation="MKKFSIGILVTIMVLAIASTASAHIVGPITRAENLALAAGFEVV
                     SHEHLPENLPIMPIVLLDGQMAMSEVGQYVITLIKRNDGRTPIEVDGSFTADAFQAYD
                     IGLVADSTIHLTISYIVLNGTPRAVQVVGVGCARWAREIPSK"
     gene            3669..3744
                     /locus_tag="PLM74_06495"
     tRNA            3669..3744
                     /locus_tag="PLM74_06495"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3702..3704,aa:Arg,seq:acg)
     gene            3754..3829
                     /locus_tag="PLM74_06500"
     tRNA            3754..3829
                     /locus_tag="PLM74_06500"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3787..3789,aa:Arg,seq:cct)
     gene            3947..5794
                     /locus_tag="PLM74_06505"
     CDS             3947..5794
                     /locus_tag="PLM74_06505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HON42764.1"
                     /translation="MLRQELQIIDAIDTQGLIVTDSGGVIVHANERARRLLGRDLPTG
                     SCIQHVCPPLTDASEFTICSERLGDALSGMPSAREVRISRRPLNLGGASDPNYMLFAL
                     EDVTLQNTLAERIKFLSSVLDRAAGGITIIDPDLKIVYVNQAQASMHRYSIGELVGKS
                     IDVFCDPEEIRRLQESIFSAGEREGTWTGEAINYAKDGTAIPVLLSVSMLRDSAGNLS
                     AIVGVTKDIHTYKELEQDMRSYSENLARLVSLRTRELESSRDELQTMFDAVSDFMFVV
                     DSSYSIIRANRFTASFFHRRRHEVIGCKCYSLWGDGTGPCDGCPAQEVFTSGNTAFAS
                     RVIKEEQVHVWAYPMRAMGHTADAPALSGVLCYGKVMTSELAMEKRIQQAEKMASLGQ
                     LSAGLAHELRNPLGTILAANYAVKTRVDSGDSEVHMAMDAIERNVDRATRIINNLLDF
                     CSKGAPSVATFSIADLASQVLALEEELLRRQGITVNVDLDSLPPVTANVDGMRQVLFN
                     VVHNAVQAMPNGGRLAVSGRAVPSGIQLTIADTGIGIGPRDLSRVFDPFFTTKAPGQG
                     TGLGLTICYREIERHRGSINISSRPGHGSEVTIELPLGSPAAWPQSGEG"
     gene            5818..>6702
                     /locus_tag="PLM74_06510"
     CDS             5818..>6702
                     /locus_tag="PLM74_06510"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HON42765.1"
                     /translation="MNSARIILVDDEPDFLHFMQLSLTDCGYRVEIATGSAEACAMVA
                     SSEFDVAVIDVRMPEMTGIELMEHMRKQGFRGQIIVLTGHGSIDGAVEAMRKGAFTYV
                     TKPIRPDDLALEIDKALRLSVITDENRRLREELAELTSRQTLIGDSEPMTRIRRLVSV
                     AAACDSPVLITGESGTGKDVVARAIHALSPRNRYRLVKVNCAAVPDDLWESEMFGHEK
                     GAFTGAVARKHGKVELADRGTLFLDEVSDIPVQVQPKFLRVLEDKCFERVGGTCTVNV
                     DFRLVCATNRQIDQEVATG"
BASE COUNT         1460 a         1993 c         1957 g         1292 t
ORIGIN      
        1 ggcgagttcg ccctggagtt cgaaggcttc ggcgaagaac cggccgagga atccgaaccg
       61 gtgtcgagca gcgatctgga tctgtacctt agcgaccagc ctgaggagcc ggaagtcgga
      121 gagcctgcga caactcgcaa gaccattgag atggaatatg agttctccaa ggactcagta
      181 atcgagagga tcgtcgattc aggaggcctg cctcacaggt tctcacagct cgaggtgctg
      241 cgtgcgccca tcaagaaggg caagacatgg agtttccaga cgcccgattg gaccgacgtg
      301 aaggccgaga tcattgagac cggcaaagac gcccataagg ccgactacgt cagggtcagg
      361 tatgaagcgg aagtggacgg cggttcatac atcgagacca ggctgttcaa gaaaggccta
      421 ggcatggtgg agttcacgtc gtctgggccc gacggggccg agttcgtgta cagcctcata
      481 ggcaactagc tcctgcggac gactgctgct gcagtcgttc gtacgatgac gcaaacaccc
      541 ctggctgcgc cgacgcgcgg ccaggggttg gtcattcatg cccatattca ggcccacaca
      601 caggcccaca cataggcctg cacactcttg ctcacgcccg ggccctgacg cgtaggcgca
      661 gacacgtcta cgaaggccta gcgccccctg caactagggc gcaataggta gcaggcgctg
      721 cgtggctgac tcaattgcgt cagcacgtgc caaagcctcg tcagcggagt cagcccgaac
      781 ggctatgtac agcttgagct tgggctctgt cccggaaggc ctcagcgtca ccttccccaa
      841 atcggtcagt ttgagctgta tgcagttctc gcgcggcagg tcgataggca ctcgaacccc
      901 cgtgccacgt cgcttggtcc atcccgtgag gtagtcgtgc acctctacaa ctggaaaacc
      961 gcccactgag tccggagtat cgcgccgcaa agactccatc gcctcgccgg ctcccacgga
     1021 cgcagcaata ggcacggata acaagcgctc gtggaaatgg ccgtactcgt ggaatagctc
     1081 atctaggcgt tcgagcaggg tcttccctct caggcgctgt cgcacggcag cacatgctat
     1141 cagggcggac gcgattaccg catccttgtc gcgaacgaac gtgcccgcaa ggtacccgca
     1201 gctctcctcg aacccaagca ggaagcggtc gccggcccgc tccatctcgc ctatcttctc
     1261 tccgatgtac ttgaacccgg tcagcgtgtc gattactctg accccggagg cggcagctat
     1321 cgcagtgacc atctcggtgc tgactatggt cttgatgatg gcatacccgg cgagatctcg
     1381 gtcagacgcg cgagcgccct cgatcaggag gtctgcgagc agcacacctg tctggttccc
     1441 ggtgagcaca cggtagcgct ctgcagcctg gtcccataca gcgacgccaa cccgatcaca
     1501 gtctggatca gttgcaagga tgatcccagg cagcgcccct cccgcatcgt gcgcctgggc
     1561 aatcgccatc tcgaaagccc cggggtcctc gggattgggg tatctcacag tcgggaaggc
     1621 gccgtccggc acagattgct ccgcaaccgt ctgggctgac gcaaacccca gctcgcgcaa
     1681 gacgcgcggg ataaacacat gccctgtgcc atgtagaggt gtatagatca agccgaagga
     1741 cgcgcgcgcc tcgcggggcg caagggcacc ggcaatccca ttaaccaggt cgagcacgcg
     1801 acgaacgtac gattcgtcga cggcagcgtc gacgtactcc accggggctt tgggctcgcc
     1861 tgggggcgag aggtaatcat ccaccgcccg cgcgcggtcc gccacctccc ctgcctcgtc
     1921 aggcagcaac tgaaccccgt gagcgtcgta agccttgtaa ccgttgtact cggaagggtt
     1981 atggctcgcg gttatgacaa tgccgcctgc cgcggcgagt tcacgcacag cgtacgacag
     2041 caccggagtc gggcggatgt catcgaagag ccacacacga atcccatgcg atgcaagcac
     2101 gcgcgctgtt tcatcggcaa actcggtcga accccgacgt ggatcgcgag ctatcaccac
     2161 gcccgcctcg cgagctccgg gcacacgagc cagcagatag gcagcgtaac catgggtagc
     2221 gcaccgaacc gtgtaccggt tcatcctggc tgtgcctacc ccaagcttac cccgcattcc
     2281 gcccgtgccg aaggccagct cgctgccgaa tcgctcttcg atctcacgtg cgtcgaagga
     2341 tatgcggtca agttcctgtc ggctggattc gtccagcatt gccgctgcat cgcgccatcg
     2401 ttcgaaaaca gctatgtaat ccatgcggta ccctccccag aacgtgcgtc acttgccgaa
     2461 tcatcatccg catcatcatg atgatagcct aacatgcatg atcccacaac atccccactg
     2521 tatcctcgcc gcatcctcgc cgtatcctca ccgcatgcca ccatgcagag caatttccgc
     2581 actggccggg cactacttcc gcgtagtctc gttcggtagg caacgcgcca cgggaaatcc
     2641 cccgctacgg caccgccagc tagagccccc gggccgccaa ggccgccgca ggccccgagg
     2701 caagcgtaat cgtcacgtgg tcatatgttc atggtgccgc gcatagctta gaacggtgta
     2761 gtgttcgcat aaccaagata ttcgcgaatt gtggtgagtc atgcggcgaa cgagaagatt
     2821 gctagcatcg actcccaaca agcgtggaat acggtactgt gacttcgaaa tgactggaaa
     2881 ctctatggtg tcatatggaa ttactggggt gacatgtgcg ctcgctcata ctactatatc
     2941 gttagcagac tggccagtcg ctgcgaataa cggactggaa ggggcgagac catgaagaag
     3001 ttctccattg gtatcctggt gacgatcatg gtgttggcga ttgccagcac agcgagtgcg
     3061 catatcgtgg gcccaatcac cagggcagag aatctggccc ttgcagctgg gttcgaggta
     3121 gtatctcatg agcacctgcc cgagaaccta ccgatcatgc ccatagtact gctcgatggc
     3181 cagatggcca tgtcggaagt agggcagtac gtgatcactc tgatcaagag aaacgatggt
     3241 agaacgccta tcgaggttga cggttctttc accgctgatg ccttccaagc gtacgatata
     3301 ggtttggttg ctgactcgac aatccacctg accatcagct acattgtgct taacggcaca
     3361 cccagagccg tacaggttgt cggagtgggt tgcgcccgct gggcccggga aatcccaagc
     3421 aagtagcctg tctgacttcg acgcaactgc tctccgtgtt cagtagcgtt tagactggct
     3481 tcgagagtag tcggtttgag cctgagggat catgggcgat tgcccgcaat cctcaggctc
     3541 gtgctgcatc ggctcgccat catcgccgcc atcacactgc cgctttcggg ttatttgacg
     3601 cctacgaatc gctcgtgcta taatggcgaa gcaaggttcg tggcgtgatc cgtcacgcca
     3661 acgcaaacgc gcccgtagct cagtggatag agtgacggac tacgaatccg caggccgcag
     3721 gttcgaatcc tgccgggcgc gccatttttc cgtggccctg tagctcaggg gatagagcag
     3781 tggtttccta aaccattggc cgcaggttcg attcctgcca gggccaccat atttagtgga
     3841 acagcgaaga caacaacgcc cgggccgtgg cccaggcgtt gctttgtatg agctgccttt
     3901 tcgggtcaga gatcaccccg gacgcaccgc taggggggac gcgcccgtgc ttcgccaaga
     3961 actgcagatc atcgacgcca tcgatacaca gggcctgata gtaaccgata gcggcggcgt
     4021 catcgtacat gccaatgagc gcgcgcgccg gcttctcggc cgtgatctgc cgactggcag
     4081 ctgcattcag catgtctgcc ctccgttgac tgatgcaagc gagttcacga tctgctccga
     4141 gcgcctcggc gatgccttga gcggaatgcc ttccgcgcgc gaggtgcgca tctcacgtcg
     4201 tcctctcaat ttgggcggtg cttcagaccc gaactacatg ctatttgcgc tagaggacgt
     4261 cacactacaa aacacgcttg ccgagcgcat caagttcctt tcatcagtgc ttgaccgagc
     4321 tgcgggcggc atcaccatca tcgatcccga cctgaagata gtatacgtga accaggccca
     4381 agccagcatg catcggtaca gtatcggcga gcttgtcgga aagagcatag acgtgttctg
     4441 cgaccccgaa gagatccgcc ggttgcagga atccatattc tccgcagggg aacgcgaagg
     4501 aacgtggacc ggcgaggcga taaactacgc aaaagacggt actgccattc cggtgctgct
     4561 ttccgtatcg atgctgcgtg attcagccgg caacctctcc gcgatagtgg gtgtgaccaa
     4621 agacatccac acctacaaag aactcgaaca ggacatgcgg tcctacagcg agaatctggc
     4681 tcgtctggtg agcctaagaa cacgtgaact ggaatctagc agagacgaac tgcagacaat
     4741 gttcgatgcg gtctcggact tcatgttcgt cgtggatagt tcgtactcca taatacgtgc
     4801 aaacagattt accgcctcgt tctttcatag gagacgccat gaggtcattg gctgcaaatg
     4861 ctacagcctg tggggcgacg gcactggccc ctgcgacggt tgccccgctc aggaagtctt
     4921 cacatcaggt aataccgcat tcgcctcccg agttatcaaa gaagaacaag tgcacgtgtg
     4981 ggcctacccc atgcgcgcta tgggccacac ggccgacgcc cctgccctat caggtgttct
     5041 gtgctacggg aaggtcatga cgtcggagct tgccatggag aaacgcatcc aacaggcaga
     5101 gaagatggcc agcctcggcc agctctcggc gggcctggcg cacgagttgc gcaatccgct
     5161 gggtactatc cttgcggcga actacgctgt gaagacccgc gtggacagcg gcgatagcga
     5221 agtccacatg gctatggatg caatcgaacg caatgtcgat agggccacta ggataatcaa
     5281 caacctcttg gacttttgca gcaaaggcgc cccgtctgtt gccaccttca gcatcgccga
     5341 cctagcgagc caggttctgg cgcttgaaga ggagttgcta cgccggcaag gaatcactgt
     5401 gaatgtcgat ctcgatagcc ttcctccggt cacagcgaat gtcgacggca tgaggcaggt
     5461 gctgttcaac gtggttcaca atgcggttca ggctatgccg aacggcggtc gacttgccgt
     5521 gtccggccgt gccgtcccct cgggcattca gttgacgata gcggacacgg gtatcggaat
     5581 cggcccacgc gacctgtctc gcgtgttcga tccgttcttt accacaaagg cgcctggcca
     5641 gggcacaggc ttgggtttga caatttgcta ccgcgagatc gaacggcatc ggggatcgat
     5701 caacatatcc agccgaccgg ggcatggatc ggaagtcacc atcgaattgc ccttaggcag
     5761 ccctgcagca tggccccagt caggcgaagg atagtgaaca aggaaagtga acgcggaatg
     5821 aacagcgcaa ggatcattct tgtggacgac gagccggact tcctgcactt catgcagctc
     5881 agcttgaccg actgcggcta ccgggtggag atcgccacag gaagcgcaga agcctgcgcc
     5941 atggtggcct cgtcagagtt cgatgtagca gtcatagatg tacggatgcc cgagatgacc
     6001 ggcatcgagc tgatggaaca catgaggaaa caggggtttc gcgggcagat tatagtattg
     6061 acaggccatg gctcaatcga cggcgccgtg gaggcaatgc gcaaaggggc tttcacctac
     6121 gtgaccaagc ccatccgccc tgacgatctc gctctcgaga tagacaaggc gcttcgcctc
     6181 tcggtcatca cagatgagaa caggcgcctc agggaagagc tcgcggaact caccagcagg
     6241 cagaccctca taggcgactc agaaccaatg acgcggattc gccgtctggt gtcggtggcg
     6301 gctgcctgtg actctccggt gctaatcacg ggcgaaagcg gcacaggcaa agacgtggtc
     6361 gccagagcca ttcacgcgct gagcccgcgg aaccgctaca ggctagtcaa ggtgaactgc
     6421 gcggcagtgc cggacgatct ttgggagagc gagatgttcg gccacgaaaa gggcgctttt
     6481 acgggggctg ttgctcggaa acacggaaag gtggaactgg cggacagggg gacactgttc
     6541 ctggacgaag tgtcggacat tccggtacaa gtacagccca agttcttgcg tgtgctggaa
     6601 gacaagtgct ttgagcgcgt cggaggaact tgtacggtca acgtagactt ccgcctagtg
     6661 tgtgccacca accgtcagat agatcaggaa gtagcaacag gt
//
LOCUS       DAOTAC010000124         6652 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_9785_length_6652_cov_4.112021, whole genome shotgun sequence.
ACCESSION   DAOTAC010000124 DAOTAC010000000
VERSION     DAOTAC010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6652
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9785_length_6652_cov_4.112021"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..1761)
                     /locus_tag="PLM74_06515"
     CDS             complement(7..1761)
                     /locus_tag="PLM74_06515"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HON42766.1"
                     /translation="MIPFVGAPGRASAALADRGRFLIFIGGDQVGVEDFELTKTSARS
                     HVEISVGGQASRAQVELALSPQMEAESYRLEAPGTYLKVAFEEDTAKFEAPGVSRRTK
                     VCRPRVVLDNNVFSHYQLLLGMYDHSRGGVQSFMAVLPTTNAADASVSVTMELLGPGR
                     PRRQARANVDADAKPDADAEARIPLTEYKIILAGVIGVSVFADGAGRVMYVEVPGQNG
                     TAVREEYQRAIEKAADRAKPARSAGPSRSAALAKSATSAASAAPAALSASVVPDYVDQ
                     DVVVKSAGDVRLAGTFTLPLAHPDQGVRYPAVVLISGSGPQDRDGNTPPSYQTFIFRR
                     IAERLASCGIAVLRYDDRGVGTSTGDFESAGLFDLAGDARAMVASVKGYPNIDPARIG
                     VIGHSEGAYIASMLAGEDPQIAACVLLAGASTTLDRVMVEQIEYQATFNELDEASRSL
                     AAGLLPAVKQLIQDARDGKSRSAVSGNLQWLREHMQIDPVGQIRAIKAPILIVQGEKD
                     LKVKPYHAQVLAEAARSAGNRSVTLVRLPNTTHEFLEWPYRNPNFDPMDPMRVVEGML
                     ATVQGWLVSTLQASSAVG"
     gene            complement(1922..2899)
                     /locus_tag="PLM74_06520"
     CDS             complement(1922..2899)
                     /locus_tag="PLM74_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HON42767.1"
                     /translation="MKELLVISGKGGTGKTSIVGSFAVLADNKVLADCDVDAADLHLL
                     LSPTVRKTNEFRASRQAVLDPARCIGPEECVGPAECVDAGQCSAWAVCAEVCRFEAIG
                     PAGRAGSAARAGLPHIVPRIDPISCEGCGVCARACPHGAISMEEVLSGHWFVSDTAYG
                     PMVHARLGVAQENSGKLVTQVRREARSIAESQSLDYIITDGPPGIGCPVISSMSGVDF
                     ALIVTEPTVAGAHDMQRAVELARHFGVKTTVCINKYDLDEAGSEQLEEYCSSQGIQVA
                     GKVPFDEEVVSALVRGVPVVARGSSHHAEGKAAQAIRRLWSSIESELAD"
     gene            complement(2896..3888)
                     /locus_tag="PLM74_06525"
     CDS             complement(2896..3888)
                     /locus_tag="PLM74_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006963874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HON42768.1"
                     /translation="MIVSVASGKGGTGKTTVAVNLALALALEGTTSVDFLDCDVEEPN
                     AHLFLHPVLETSEPVTLPVPVVDESKCSGCGICADVCAFHAILSFGNRAITFPELCHG
                     CGGCVRFCPEGAISEAPREIGVVETGWVSCVGAPAVAARAAEAAGSVGIARSSGLLVS
                     AGSTAAAQAIRFVQGRVDIGAALAPPVAKAVRGKVGPANGVVIIDSPPGTSCPVVASV
                     EGSDYCILVTEPTPFGLNDLTLAVDMVRELAVPFGVVINRAGLGDARVTAYCRQENIP
                     VLLEIPFDRRFATSYARGGQLVRDFPELEESMRGLWRQIRTVAGPLPEEAEMAR"
     gene            complement(3892..4335)
                     /locus_tag="PLM74_06530"
     CDS             complement(3892..4335)
                     /locus_tag="PLM74_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HON42769.1"
                     /translation="MKIALATDGAVISAHFGHCSEFTVVTVENGKTVGTERVANPEHQ
                     PGFLPEYLSGLGVSCIIAGGMGPRAQELFAAKGIQTIVGASGSVTKVLGDYLAGSLEV
                     GESACDHADSSERYAGGQAEGHEGHGSCSHGGHNDHHGHSCSHGR"
     gene            complement(4402..4773)
                     /locus_tag="PLM74_06535"
     CDS             complement(4402..4773)
                     /locus_tag="PLM74_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HON42770.1"
                     /translation="MRIAITAQGEDLDSAMDPRFGRCRSFIIVDSDSDKFEAFSNEGV
                     MASGGAGTQSAQFLANKGVGVAITGNVGPNAARALEAAGIKVYAAASGTVRDALQAYR
                     AGKLSAVSGATVDSHHGMGRR"
     gene            complement(4775..5167)
                     /locus_tag="PLM74_06540"
     CDS             complement(4775..5167)
                     /locus_tag="PLM74_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HON42771.1"
                     /translation="MPRGDGTGPAGFGPMTGRAAGYCAGYDIPARTGAGRGRGHRRMY
                     YSTGMPGWMRYGCPGSAPFPVGGFRGASQESRHADDVQGMSAEREKELLGQQAQFLRS
                     ELEIVERRLNDLAGLGRNPEQRSTEGGE"
     gene            complement(6043..6330)
                     /locus_tag="PLM74_06545"
                     /pseudo
     CDS             complement(6043..6330)
                     /locus_tag="PLM74_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031759.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
     gene            complement(6300..>6652)
                     /locus_tag="PLM74_06550"
     CDS             complement(6300..>6652)
                     /locus_tag="PLM74_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42772.1"
                     /translation="ARAEKIQLSTVERPPAGASADRLSVDERERAKECVDDRAGLISS
                     PGLPGVSEESILIGGEGEQQHRRRSNAAALSISLGEGLIDAIRRDDGLTYFGHAHDSE
                     QELIPCQDRPNSDA"
BASE COUNT         1247 a         2212 c         1866 g         1327 t
ORIGIN      
        1 cacacactaa cctacagccg atgaagcctg caaagtcgaa acgagccatc cttgcacagt
       61 ggcaagcatg ccttctacta cgcgcatggg gtccatcggg tcgaagttcg ggttccgata
      121 cggccactca agaaactcat gagtagtgtt gggaagacga actagggtaa cgctgcgatt
      181 gcctgcgctt cgagccgcct ccgccaggac ctgcgcatgg tatggcttga cctttaggtc
      241 cttctcgccc tgcacaatta ggatcggggc cttgatggcc cgaatctggc ccaccgggtc
      301 tatctgcatg tgttcgcgca gccactgcaa gttacctgat accgcgctac ggctcttgcc
      361 gtcgcgcgcg tcctgtatga gctgcttcac cgcaggcagc aggccggcgg ccagcgaacg
      421 cgaggcctcg tccagctcat tgaaggtcgc ctggtactcg atctgctcta ccatcactct
      481 gtccagagta gtggacgcgc ccgccagaag cacgcacgcc gcaatttgcg ggtcttcgcc
      541 ggccaacatg gaggctatgt aggctccctc cgagtggccg attactccga tgcgggccgg
      601 gtcgatattg gggtagccct tgacagacgc gaccatcgcc cgggcgtcgc ccgccagatc
      661 gaacaacccc gccgattcga agtcgccggt gctcgtgcct acccccctgt cgtcgtatcg
      721 aagtaccgct atgccgcaag atgccaggcg ctcggcgatc cggcggaata tgaacgtctg
      781 gtaggacgga ggcgtgttcc cgtccctgtc ttgcggaccc gatccggaga ttagcacaac
      841 ggcagggtag cgcacaccct gatccgggtg ggctagagga agggtaaaag tccccgccag
      901 gcggacatcg cccgcgctct ttactactac atcctgatcc acatagtcgg gcacgactga
      961 tgcggataac gcggcgggtg cggcagatgc ggccgaggtt gccgatttgg ccaaagcggc
     1021 cgaccgggac ggccctgccg acctggccgg cttggcgcgg tcagcagcct tctcgatggc
     1081 cctttgatac tcctcccgca cggccgtgcc attctggcct ggcacttcca catacatgac
     1141 tcggcctgcg ccatccgcga acaccgacac gccaatcaca cccgccagta ttatcttgta
     1201 ctcagtaagc ggaatccgag cctcggcatc tgcatccggt ttcgcatccg catccacatt
     1261 cgcccgtgct tggcgcctgg ggcgccctgg cccgagcaat tccatggtga cagagactga
     1321 ggcgtctgcg gcatttgtgg ttggcagaac cgccatgaag gactggacac ctccgcggga
     1381 gtggtcgtac atacccagaa gtaattgata atgactgaac acattgttat ccagaaccac
     1441 tcgcgggcga cacaccttcg tccggcgaga cacccccggc gcttcgaact tagcggtgtc
     1501 ttcttcgaag gcgaccttga ggtacgttcc cggcgcctcc agtcggtagg actcggcctc
     1561 catctgcgga gatagagcta actcaacttg ggcgcgcgaa gcctgccctc ctacggatat
     1621 ctcgacatgc gaacgggcgc tcgtctttgt tagctcgaag tcctcgacac ctacttgatc
     1681 tccgccgatg aaaatgagga accgaccgcg gtcagcgagg gcagcagaag cgcgccccgg
     1741 cgcgcccaca aaaggaatca taatcaacac caccagcagg gctgccgtca gtacccgcgg
     1801 gccagcggac gtacctacat cagaatcggg ccgacggcgc cttccactat acgcgcgaaa
     1861 ggcccgtgga gtgtcacttc cgtatccgtg cccccgccct gctgtgtcag gtttccttct
     1921 gctagtctgc cagttcgctc tcgatactcg accagagacg gcgaatggcc tgcgctgctt
     1981 taccctccgc gtggtgggaa ctgccgcgcg ccaccacagg cacgcctcgt acaagggccg
     2041 agaccacctc ttcatcgaac ggaaccttac ccgctacttg gatgccttgg ctgctgcagt
     2101 actcctctag ctgttcagag cctgcttcgt cgaggtcata cttgtttatg cagacagtgg
     2161 tcttcacgcc gaagtggcgt gccaactcaa ccgctctttg catgtcatgc gcccctgcaa
     2221 ctgtaggttc agtgacgatg agcgcaaagt ccactccgga catggacgat atgaccgggc
     2281 agcctatgcc gggcggtccg tctgtgatga tgtagtctag actctggctt tcggcgatcg
     2341 accgcgcctc gcgcctgact tgtgtgacta gcttgcccga gttctcctgg gcgactccga
     2401 ggcgtgcgtg gaccatgggt ccgtaagctg tgtcggacac aaaccaatgc cccgacaaca
     2461 cttcctccat cgagatggcc ccgtgaggac atgcgcgggc acagaccccg cagccctcgc
     2521 aagagatcgg atcgatccgg gggacgatgt ggggaagacc ggcgcgggca gcagaacctg
     2581 ccctcccggc cgggccgatg gcctcgaacc tgcagacctc ggcgcagaca gcccatgcgc
     2641 tgcactggcc cgcgtcaacg cattctgccg ggccaacgca ctcttccggg ccgatgcatc
     2701 tggctgggtc tagcaccgcc tgtctggacg cccggaactc gttggtcttc ctcactgtcg
     2761 ggctgagcag aaggtggagg tcagctgcgt ccacatcgca atcggctagc accttattgt
     2821 ctgccagaac agcgaaggat ccaactatgg aggtcttgcc tgtaccgccc ttgccgctga
     2881 tgacaagcag ttccttcatc gtgccatttc cgcctcctcc ggcagcggcc ccgccacggt
     2941 gcgaatctgc cgccagaggc ctctcattga ttcctccagc tcagggaagt cgcgcacgag
     3001 ctggcctcca cgagcgtagg aagtcgcgaa tcttctgtcg aatgggatct cgagcagcac
     3061 cgggatgttc tcctgcctac agtaggccgt tactctggcg tcgcccagcc ctgctcggtt
     3121 gataaccaca ccgaagggga cggctagttc gcgaaccata tccactgcga gtgtcaggtc
     3181 gttcagccca aacggcgtcg gctcggtaac gagtatgcag tagtcgctgc cctctaccga
     3241 ggccacgacg gggcaggacg tcccgggtgg cgagtcgatg atgaccaccc cgttggctgg
     3301 gccgacctta ccccgaacag ccttcgctac tgggggagcc agagcagcac cgatgtcgac
     3361 tcgtccctgg acgaatctga tcgcctgtgc cgctgccgtc gaccctgccg aaaccaacag
     3421 tcctgacgat cgtgcgattc caactgatcc tgccgcttcc gcggctctcg ctgctaccgc
     3481 cggcgcgccc acgcagctta cccagccggt ttccacgacc ccgatctccc tgggcgcctc
     3541 ggatattgcg ccttctgggc agaaccgcac acagccgcca catccgtggc acagctcggg
     3601 gaaggtgatc gcgcggtttc caaacgacag gatcgcatga aacgcgcaca cgtccgcgca
     3661 gatgccgcat ccgctgcact tgctctcgtc aacgacaggc acaggcaggg tgactggctc
     3721 ggaggtctct agcacgggat ggagaaacag atgagcattg ggctcctcga catcacagtc
     3781 cagaaagtcc acagatgtag ttccctccag agcaagagca agagccaggt taaccgctac
     3841 agtcgtcttg cctgtgccgc cctttccgct ggcaactgag acaatcatgg cctacctgcc
     3901 gtggctgcac gaatgtccat ggtgatcatt gtggccgccg tgactgcagg acccgtgccc
     3961 ttcgtgtcct tcagcctgtc ctccagcgta tctttcggag ctatcagcgt gatcgcatgc
     4021 actctcgccc acttcgagcg atcccgcgag gtagtcgccg agaaccttgg ttacactccc
     4081 cgaggcgccg actatggtct ggatgccctt ggcggcaaac agctcctgtg cccttgggcc
     4141 catgccgcct gcgatgatgc acgaaacccc cagtcccgat aggtactccg gcaggaaccc
     4201 cggctggtgc tccggattag ccacgcgctc tgtgcccacg gtcttgccgt tctctactgt
     4261 gactacggta aactcggagc aatgaccgaa atgcgctgat atcactgcgc cgtctgttgc
     4321 caatgcgatc ttcatatgtg atgtacctcc caaatcgctg caaatcattg agttggtcta
     4381 gctgctaata gtctcagtcc tctagcgtct tcccattccg tgatgggagt ccacagtggc
     4441 tccggagacc gcgctgagct tgccggccct gtaagcctgg agcgcatcgc gcaccgtgcc
     4501 tgaagccgca gcatacacct tgatacctgc cgcttccagc gcgcgtgctg cgttagggcc
     4561 gacgttgccc gtgatggcca cgcctactcc cttgttagcc agaaactgcg cgctctgagt
     4621 gcccgcgcct ccagacgcca tgacaccctc gttcgaaaac gcctcgaatt tgtcggagtc
     4681 ggagtcgacg atgatgaagc tccgacacct cccgaaccgc gggtccatag ccgaatccag
     4741 gtcctcaccc tgagcggtta tcgcaatacg catgttactc gccgccttcc gtcgagcgtt
     4801 gctctgggtt ccttcccaag ccagccaggt cattgagtcg ccgttccacg atctcaagct
     4861 cgctcctgag gaactgcgcc tgctggccga gcaactcctt ctcgcgttcc gccgacatgc
     4921 cctgaacatc atcggcatgg cggctctcct gcgacgcacc tcgaaaaccc ccaacaggga
     4981 acggagccga tccggggcag ccgtacctca tccagcctgg catgcccgtg gagtagtaca
     5041 ttcgcctgtg gcctctgccg cgacccgccc cggtccgcgc aggaatgtcg taccctgcac
     5101 aatagcccgc agctcgtcca gtcatgggcc cgaagcctgc cggacccgtt ccatcgcctc
     5161 tcggcatatc ccatacctcc ttagctataa tgatcatatg cccgttatca gtatacccac
     5221 agttatgggc atatgtcaat aaccaaggcg gcggaacgac cgagatggcg aaaagaggtc
     5281 ctacacaggg tcccgccacc aaaggcgtgc tgagcatggc tttgtctggc ctgcgggaat
     5341 accgtacgga ctcttcggaa gcctggtgga atatcagacg gttccgcacg gccggacgag
     5401 atcggaaggc gtcttgggag tcgttcagct cgtatcggct gtctcggacg gctggtgttg
     5461 acaccgccct gaagacacca tgcctgtcca gcggctatat tctgccgtat tcgcgcagcc
     5521 cgaatcccag gtaatgtttt gggcgactcc ttcctgcggt tctatacagc tatacgttcg
     5581 attatgcagc acctattgtc accaaacgga acagatcgga gaaattgtgt tcggaatctg
     5641 aacagcctga tttcctacag ctcggcgcgt ccgccttgtc gcccatgcga gcatcagtca
     5701 cctgcccccg cagcgccccc gcccctttcc tccgggtggc gatcccgggt cggaacacgg
     5761 gccggggtcg gggtcgcagt catgtctggg gtagggatcg tgtccagggt caggaccagg
     5821 gtcgcggccg gagtcggaac cgcgtccgga atcagggtca gggccatgtc cggacccatg
     5881 ttcgggtccg tgcctgtgcc catgcccaca tccggggcgc ccaatcctct ttccccgctc
     5941 agccccgcag tgtgggcatg cggggtctac cccactccgc gctgcggcga atggggcctc
     6001 gaactcatgg ctacacgccc cacaccggaa ctgccccaga gccagtcggt aggtcccgcc
     6061 ttcgaagcga atggcccttc cctccacaag cgcccgagcc actttctccc tggccgatga
     6121 gagcactctc tgaaacgtag gccgagacac ctgcatacgt ccagcgcact cttcctgctc
     6181 cagtccttca agatccttga ggcgtatagc ctcaagctcc tctactgtaa gcacttcctc
     6241 ttccagatca cgcatcgaca cacccgcagg cttgaatacg gtcacctgag ggatgaactc
     6301 tacgcgtctg agtttgggcg gtcttggcat ggtatcagct cctgttcaga atcatgcgca
     6361 tgtccgaagt aggtcaaccc gtcgtcacgg cggatggcat cgatcagtcc ctcgcctaag
     6421 gagatcgata atgctgcggc atttgatcgt cggcgatgtt gctgttcgcc ctcgccgccg
     6481 atcaggatgc tttcctccga aacacccggc agacccggtg aggatatgag ccccgctcga
     6541 tcatcgacgc actccttagc ccgctccctc tcgtccaccg acagccgatc ggccgaagcc
     6601 ccagcgggag gtcgttccac cgtgcttagt tggatcttct cagctctggc gc
//
LOCUS       DAOTAC010000125         6621 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_9839_length_6621_cov_4.345720, whole genome shotgun sequence.
ACCESSION   DAOTAC010000125 DAOTAC010000000
VERSION     DAOTAC010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6621
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9839_length_6621_cov_4.345720"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..1443)
                     /locus_tag="PLM74_06555"
     CDS             complement(172..1443)
                     /locus_tag="PLM74_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HON42773.1"
                     /translation="MNTVAVVVGGGWAGCAAAVAAAQAGADVVLLERTNLLLGTGLVG
                     GIMDNNGRFTAARELAAMGAGYLFEVIDQVTIHRRVDFPGHLHASLYDVNRVEAAVRR
                     ALTELGVHLMLESRAVDVEKEGDRISRLVLSDGQRVPAHCFIDATGTAGPQGNCARVS
                     GGCVMCALRCPAFGPRTSIAGKAGAREISGSLFEALSGSCELQKKSLAPWLARNLEEH
                     GALVIPVPRYVPRDDRSAPKACQQYSLPEFYDNLVLLDTGHAKLMAPFFPMDRLRAIP
                     GFESALYQDPYAGGRGNSIRFTVITPHDLSMKVTGVENLFCAGEKAGLMVGHTEAICT
                     GTLAGHNAIRKTAGKEPLILPSSLACGDLLACIERSLGSPSGLLNKYTFAGGVYYNRM
                     YDSGLYTTDIRRISQRVRDAGLTGIFSRRLL"
     gene            complement(1440..2333)
                     /gene="dpsA"
                     /locus_tag="PLM74_06560"
     CDS             complement(1440..2333)
                     /gene="dpsA"
                     /locus_tag="PLM74_06560"
                     /inference="COORDINATES: protein motif:HMM:NF006162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HON42774.1"
                     /translation="MADLRGLSICVLGGDRREVELVSGLIDSGAHVSCFGTPTEGMRS
                     EAVAALSARDACAGANAVILPLSGTDTNGNIKITSRPVSINRELLAAADPAAIVLSGV
                     VRPCLAADCAALGLRVVDTGEDDELAILNSIPSAEGAILMAMQATPFTIHNSNCLVLG
                     PGRTGLTLVRMLQGLGAHVWGAARKAPGRARLFEMGAMPVDFADLPHCLPQIDIVFNT
                     VPAQVLDRSLVYVLRRTAVIIDLASAPGGVDFVAAAERGIHAELAPGLPGKVAPLTAG
                     RILARVIPRIIEDELRHERVR"
     gene            complement(2389..4881)
                     /locus_tag="PLM74_06565"
     CDS             complement(2389..4881)
                     /locus_tag="PLM74_06565"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HON42775.1"
                     /translation="MKAMLLGAGEGTRLRPITATRPKPMISIANRPIMEHILLLLKAH
                     GVREVYSNLYYLADQIESYFGDGSAQGLSVKFKVEEKLPGTAGGVKNLEQHFDETFIV
                     ISGDLLTDFDLTKALEFHRKQGSIATILLTKIDNPLEYGVVITDKSGRIQRFLEKPGW
                     SEVFSDTINTGIYILEPEVLKFIPPGQDFDFSRDLFPLLLREKQPLFGHVADGYWCDV
                     GNIEMYIRAQVDALSGKVRLSIPGEQVMPGVWVSKSAKISSRADLKGPMVLGENCEIA
                     PGARLREYCVVGDNVIVAANSFIVRSTVFSNSYIGEGSVVSGSIICENVVLKDSVRVG
                     EGAVVSDDSILNTAVTVKPGVRVWPGKIIDESVVLSASVVWGQRLQRELFHAGAISGL
                     VNFEFTPEFAVRLGSAIGSTMKKGASVIVSRDPSRTARMMKRALVAGLSSAGVSVCDL
                     QGQPLPLLSYMTCRNGYAYAVHTQTSEDNFEMVDIKVFDCDGLALSRGDERKIETLLA
                     REDPRRTSSREVGSISYYPRAVEVYTDDCLAHINVEVTKANRMRLVVDCGHGFTGSIL
                     PPLLAKMGAEVTSLNAIEQETKMPKTRRESERAREDLALIVKTIGANMGILIDPQGAR
                     MWVADDSGRVLTDMELAYMMALFGVRSKSSRGVTAPSYIPTVLTEALTSNGATVYRAK
                     SHARELSDLARTSKSGIASDLAGGFVFPELHNGMDAIFAAAKLVEWVSADSAPLSQLV
                     QLVPEHHLLRDTMPCPWELKGQAMRELLGKLDQPGVDTFDGYRKAAGDGWYMVLPDAK
                     HPSFTIYAEGPTRLDAEKILKEARRELAAQID"
     gene            5110..5898
                     /gene="folE2"
                     /locus_tag="PLM74_06570"
     CDS             5110..5898
                     /gene="folE2"
                     /locus_tag="PLM74_06570"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004099622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HON42776.1"
                     /translation="MQDVQSRPDSRGIDIQKVGVKNVHLPLQIATKEGGWQSVLGNAS
                     LSVELPMQFKGTHMSRFIEVLLSWSQKPISNVSITSILRETCESLSATKAHISIRFKY
                     FVPKCAPVSGRESILDYNCEFSGAVDGSRHTFIMGVEVPVTSCCPCSKEISSFGAHNQ
                     RTMIRARVRFSAAGFLWLEDLIALLEAQGSCEIYPLLKREDEKFVTEAAYTNPKFVED
                     ILRDVVISLRAEPRVRWFEVECESHESIHNHSAFACHVEEKRTV"
     gene            5905..>6621
                     /locus_tag="PLM74_06575"
     CDS             5905..>6621
                     /locus_tag="PLM74_06575"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HON42777.1"
                     /translation="MPVLEVGSVTKYYGSDLVLSGINVSVEERDHIGLVGRNGTGKTT
                     LLRIMAQALDYDSGSVTVAPGCTVGYLAQGIGYNSGNTLYEEMRGAFARAFRIADEMR
                     ELEHQMADPKMADPNVAGSERLLDRVMQRYSRLSTEFEMCGGYDYDVRIKTTLFGLGF
                     REDDLGRVIDTLSGGQKVRVALARMLCSAPNVLLLDEPTNHLDVDACEWLEGYLRSFA
                     GAFVVVSHDRYFLDATTNKTW"
BASE COUNT         1258 a         1954 c         2035 g         1374 t
ORIGIN      
        1 gaggcggtac ttggcgtact cccatgacct tgcccaaatc cacaggaggt tggggaggct
       61 cttcgggagg ccgctctgga ggcagttcgt ccggaggctt cggcggattc ggcggcggtc
      121 gctcaggagg cggcggcgcg aagggcggat ggtagtctcg cccgtctaca ctcagagcaa
      181 tcgacgtgag aagatgcctg tgaggccggc atccctgacc cgctgggaga tgcggcggat
      241 atcggtggtg tacaaccccg agtcatacat tcggttgtag tatacgccgc cggcaaaggt
      301 gtacttgttg agcaaccccg agggcgaacc gagtgaccgc tcgatacagg cgagcaggtc
      361 gccgcaggca agcgatgagg ggaggatcaa aggctctttg ccggcggttt ttcgtattgc
      421 attgtgccct gcgagcgtgc cggtgcagat cgcctcggta tgccccacca tcaaacccgc
      481 cttttcgcct gcgcagaaca ggttttccac tccggtcacc ttcattgata gatcatgtgg
      541 ggtaatcact gtgaacctga tggaattacc tcgccctccc gcataagggt cctggtagag
      601 agctgactcg aatccgggaa tggcccggag tcgatccata gggaagaaag gcgccatcag
      661 cttggcatgg ccggtatcga gcagcaccaa attatcgtag aattcgggca gcgaatactg
      721 ctgacatgcc ttgggcgcag aacgatcgtc ccgtggcaca taccggggca ccggaatcac
      781 cagtgcaccg tgttcttcca ggttgcgcgc aagccatggg gccagcgact tcttctgcag
      841 ttcgcaggag ccgctcaagg cttcgaacag gctgcccgat atctccctgg ccccggcttt
      901 tcccgcgatg ctggtgcgcg ggccgaaggc ggggcatcgc agcgcgcaca tgacgcatcc
      961 gccgcttaca cgggcgcagt tgccctgcgg ccctgcggtg cctgtagcat cgatgaagca
     1021 gtgggcgggc actctctgtc cgtctgatag cacgagccga ctgatgcggt cgccttcttt
     1081 ctctacgtct accgcgcgag attcgagcat caaatggacc cccagttctg taagggccct
     1141 gcgcactgcg gcttccactc tgttgacatc gtataggctc gcatggagat ggccggggaa
     1201 gtccacgcgc cggtggatgg tcacctgatc gatcacctcg aacaggtaac cggcgcccat
     1261 tgccgcaagc tcccgcgcgg ccgtgaacct cccgttgttg tccattatcc cgcctaccag
     1321 gcccgtcccg agcaagagat tggttctttc gagaagcacc acatctgctc cggcctgcgc
     1381 cgcggctacg gccgcggcgc agccagccca tccgccgccg acaaccacag ccacggtgtt
     1441 catctgaccc gctcgtgcct gagctcgtct tcgatgatcc tggggataac cctggctaga
     1501 atgcgaccgg cggtgagcgg agccaccttc ccgggcagcc cgggagctag ctcggcatgg
     1561 atgccgcgct cagccgctgc cacgaaatcg acgccgccgg gcgccgacgc cagatcgatt
     1621 atgacggctg tgcgccggag cacatacacg aggcttctat cgagtacctg agccggtact
     1681 gtgttgaaca cgatatctat ctgcggcagg caatgaggca ggtcggcaaa atccacgggc
     1741 atggctccca tctcgaagag cctggctctg cccggggcct tgcgcgcggc gccccacacg
     1801 tgcgcgccca gaccctgaag catccttaca agtgttaggc cagtgcgccc tggccccagc
     1861 acgaggcaat tgctgttgtg tatggtgaac ggcgtggcct gcatcgccat gagtatggcg
     1921 ccttccgccg acgggataga gttgaggatg gccagttcgt cgtcttcgcc cgtatccact
     1981 acccgaagac ccaaggcggc gcagtccgct gcaaggcatg gccggacaac gccgctcagc
     2041 actatggccg ccggatcggc tgcggcgagg agttctcgat tgatgctcac aggtctggaa
     2101 gtgatcttga tgtttccatt cgtgtccgtg cccgaaagag gcaggatgac ggcgttcgcc
     2161 ccggcgcatg cgtcgcgcgc gctcaaagcg gctaccgcct cactgcgcat accttctgta
     2221 ggcgttccga aacaagacac atgcgccccg gaatcgatca ggccggacac taactccact
     2281 tcgcggcggt cgccgcccaa gacacatatg gaaagccctc tcaagtcggc cacgcggtcc
     2341 gcctccttca tactcgctca gagtaagtgt atgacggagg tgccgccctc agtcgatttg
     2401 cgcggcgagt tcgcgccggg cctctttcag tatcttctcc gcgtcgagcc tggtcggtcc
     2461 ttcagcgtag atggtgaacg acgggtgctt ggcgtcgggc agcaccatgt accatccgtc
     2521 gcctgccgcc tttctgtaac cgtcgaatgt gtccactccg ggctgatcca gcttgcccag
     2581 gagctctcgc atggcctggc ctttgagctc ccatgggcag ggcattgtgt cgcgaagcag
     2641 gtggtgctcc ggcaccagct ggacgagttg cgaaagtggc gccgagtcgg ccgataccca
     2701 ttccaccagt ttggcagcgg cgaatatcgc gtccatgccg ttgtgcagct cgggaaagac
     2761 gaatccgccg gcaaggtcgg aggctatccc cgacttgctg gtgcgcgcga ggtctgaaag
     2821 ctcgcgcgcg tgcgatttgg ctctatacac tgtggctcca ttgctcgtta gagcctcggt
     2881 gagcacggtc gggatgtagc tgggcgccgt tacgccgcga gaagacttcg aacgcacgcc
     2941 aaacagcgcc atcatgtatg caagttccat gtcggtgagc actcggcctg agtcgtcggc
     3001 tacccacatc cttgctcctt gcggatcgat gaggattccc atgttcgctc cgatggtctt
     3061 gactatcaaa gctaggtctt cgcgggcacg ctctgactcg cgacgcgtct tgggcatctt
     3121 cgtctcctgt tcgatggcgt tgagcgaggt cacctcggcg cccatcttgg ctagcagcgg
     3181 agggagtata ctcccggtga agccatgccc acagtcaacc actagcctca ttcgattggc
     3241 cttggtgacc tccacattga tatgggcgag gcagtcatcg gtataaacct ccaccgcccg
     3301 cgggtagtag ctgatgctgc cgacctcgcg ggagctggtg cgccgcgggt cctcgcgggc
     3361 gagcaaggtt tcgatcttgc gttcgtcgcc gcgtgagagc gcaagcccat cgcagtcgaa
     3421 cacctttatg tccaccattt cgaagttgtc ttcggaagtc tgggtatgca cggcgtacgc
     3481 atagccgttg cggcatgtca tgtacgagag cagcggtaga ggctgcccct gcagatcgca
     3541 tacggacacc ccggcgctgg acagccctgc aaccagcgcc cttttcatca tgcgcgccgt
     3601 gcggctgggg tcgcggctaa cgatcacgct ggcgcccttc ttcatggtgc tgccgattgc
     3661 actgcccagg cgcactgcga actcgggagt aaactcgaag ttgacaaggc cggatattgc
     3721 gccggcatgg aacagctctc gctgcaggcg ctgcccccac accacgctgg cagagagcac
     3781 aacgctttcg tcgatgatct tgcccggcca aacacgcact ccgggcttga ctgtgactgc
     3841 agtgttcaga atggagtcat ctgacaccac agccccttcg cccactcgca cactgtcttt
     3901 gagcaccaca ttctcgcaga tgatggagcc cgacactacc gatccttcgc ctatgtagga
     3961 gttgctgaag acagtggacc tcactatgaa ggagttcgcc gccactatga cgttgtcgcc
     4021 aaccacgcag tactcgcgaa gacgggctcc gggggcgatc tcgcaattct cccccagcac
     4081 catagggcct ttgaggtcgg cccgcgaact gatcttggct gatttggaca cccatacgcc
     4141 gggcattacc tgttcgccgg gtatgctcaa tcggaccttg cccgatagcg cgtccacctg
     4201 agcccggatg tacatctcga tgttgcccac atcacaccag tagccgtcgg ccacatgtcc
     4261 gaacagtggc tgcttctctc ggagcaagag agggaagagg tcgcgcgaga aatcgaagtc
     4321 ctgcccggga ggtatgaact taagcacttc gggctcgagg atgtagattc cggtgttgat
     4381 ggtgtctgaa aacacctccg accaaccggg cttctccaag aaccgctgga tgcgaccgct
     4441 tttgtcggtt atgaccacac cgtactccag gggattgtct attttggtca gtaggatggt
     4501 agcgatggat ccctgctttc ggtggaattc gagcgccttt gtgaggtcga aatcggtcaa
     4561 aaggtcgccg gagatcacta tgaacgtctc gtcgaagtgc tgctctaggt ttttcacccc
     4621 gcctgcggtg ccgggcagct tctcctcaac tttgaacttg accgaaagcc cctgagctga
     4681 gccgtcgccg aagtacgact cgatctggtc ggctaggtag tacaggttag agtacacttc
     4741 gcgcacaccg tgcgctttga gcagaagaag tatgtgctcc atgatgggcc ggttggctat
     4801 ggagatcatg ggcttgggcc gcgtggcggt gatcggtcga aggcgggtcc cttctcccgc
     4861 cccgaggagc atggctttca tggcttcaga cctccgttcc tctgccgtac ttgtttgtgc
     4921 atttcgacgg cgacacgtgg ttgtcctttg gccgatgctc tggccgcgtc ctatggcggc
     4981 ctgaggttcc ggtcgctgtg ggcggaccgg gcggatgcag ggatattcct cacctgcgta
     5041 gaacagatat gagtcagtcc ttggcccaag cgaagggggg cgggccgggt gggggaggag
     5101 gagctttcta tgcaggacgt gcagagccgt ccggactcca ggggcatcga catacagaag
     5161 gtcggagtaa agaacgtgca tcttccgctg cagattgcca ccaaggaagg tggctggcag
     5221 tctgtgctgg ggaacgcctc gctctcggta gagctcccca tgcagtttaa gggcactcac
     5281 atgagcaggt tcatagaagt gctgctttcg tggagccaga agcccatatc gaacgtgtcc
     5341 ataacgtcaa tactgagaga gacgtgtgag tcgctatcgg ccaccaaggc gcatatttcg
     5401 ataaggttca agtacttcgt gccgaagtgt gcaccggtga gcggcaggga gagtattctc
     5461 gactacaact gcgagttcag cggagcagtc gacggttctc gccacacgtt catcatgggt
     5521 gtggaagttc cggtcacctc gtgctgcccg tgcagcaagg agatatcgtc ttttggggcc
     5581 cacaaccagc ggacaatgat ccgagcgcgc gttcggttca gcgccgcggg gtttctgtgg
     5641 ttggaggatc tcatagcgct tctggaggcc cagggcagct gtgagatata tccgctgctc
     5701 aagcgggagg acgagaagtt cgtcaccgaa gcggcctaca ccaaccctaa gtttgtggag
     5761 gacatattac gagacgtggt catatcgctc agggccgaac cgcgcgtgcg ttggtttgag
     5821 gtggagtgcg agagccatga gtcgatccac aaccattcgg cctttgcctg ccacgtcgaa
     5881 gagaagagga cggtgtagcc gccggtgccg gtgctggaag taggttcggt cactaagtac
     5941 tacgggtctg acctagtgct cagcgggatc aatgtgtcgg tggaagagcg tgaccacata
     6001 ggcctggtcg ggcggaacgg cacaggcaag accactctgc tccggatcat ggctcaagcc
     6061 cttgactacg attccggttc tgtgacggtg gcgccgggtt gcaccgtagg gtatctcgcg
     6121 caggggatcg ggtacaactc tggcaacacg ttgtatgaag agatgagggg ggctttcgcg
     6181 cgcgcttttc gcatcgccga cgaaatgcga gagttggagc atcagatggc cgacccgaag
     6241 atggccgacc ccaatgtagc cggatccgaa aggcttctag accgggtcat gcagcgttac
     6301 tccaggctct ccactgagtt cgagatgtgc gggggctacg actacgacgt acgcatcaag
     6361 actacccttt ttggactggg ctttcgcgaa gacgatttgg gtcgagtcat cgacactctg
     6421 agcggaggtc agaaggtccg cgttgcgctg gcgcgtatgc tctgttcggc ccctaatgtt
     6481 cttttgctcg atgagcccac caaccatctg gatgtggatg catgcgagtg gctcgaaggc
     6541 tatctgcggt catttgcagg ggcattcgtc gtcgtatcgc acgaccgcta tttcctggat
     6601 gctactacga acaagacatg g
//
LOCUS       DAOTAC010000126         6611 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_9858_length_6611_cov_5.112874, whole genome shotgun sequence.
ACCESSION   DAOTAC010000126 DAOTAC010000000
VERSION     DAOTAC010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6611
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9858_length_6611_cov_5.112874"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="PLM74_06580"
     CDS             <1..490
                     /locus_tag="PLM74_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HON42778.1"
                     /translation="QAVGRAAEGIVNEVRRQFREIPGLMEGKARPDYARCVDMCAGAA
                     QKEMVAPSLIAIIAPILVGVLLGPDGVGGLLVGTVVTGFLLAVMMANAGGAWDNAKKY
                     IESGQYGGKGSDAHKAGVVGDTVGDPFKDTSGPSLNILIKLISMVSIVFSSVIVALSI
                     IK"
     gene            750..2081
                     /locus_tag="PLM74_06585"
     CDS             750..2081
                     /locus_tag="PLM74_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HON42779.1"
                     /translation="MHVSRKQNLLIGLLLMTVVVACSGVGLAEEYVLSDSTKAVYGKL
                     MATPAVKQGIEFLFADDANTLAEQIEICEIPAPPFKEEVRAAEYMKRLAALGLKDVQL
                     DAEGNVFGVLPGTGNGPKLLVCAHLDTVFPEGTDVTVKVKDGRYSAPGIGDDTRGLAA
                     LLSVIRAFNETGIKPVGDVIFCGNVGEEGLGDLRGVKALFRDHKDIDGFITVDGDGAG
                     WILYLATGSHRYEITFNGPGGHSFGAFGLPSAIHAMGRAIAKIGDLQTPKEPRTTFTV
                     GVVEGGTSINSIAAQAKMWVDMRSNELPPLLAIEKELLAAVKQAVVEENARWNSDKIT
                     VDIKMVGDRPAGTQPADTSIVQAMYAATVALGIEPELEGPASTDANLPISLGIPALCI
                     GGGGRGGNGHAFDEWYENVDAYLGTQNIFLTILGLAGVDGVTQPLLEVRAK"
     gene            2190..3404
                     /locus_tag="PLM74_06590"
     CDS             2190..3404
                     /locus_tag="PLM74_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HON42780.1"
                     /translation="MKLWVKILIGFALGAVVGLIVGPSIAVIKPLGDLFIRLIKMLIV
                     PMVFCSLVVGASSIGDLKKLGRVGGKTVLYFLVTTAFAITLAILLSLIIDPSAGYVIS
                     EVGTYEAKAMPSIATTFLNMVPTNPINAAATDNILQIIVFALFMGIGISLLGEKGKPA
                     LAFFDSVAEIMYKITAIVMGFAPIGVFALIAWVTGTSGADVLLPLAKLIFVCYLATAL
                     HAVIVYSGAVYIWAKMTPITFFKGFFEAMAVAFSSCSSSATLPISMRCAQENLGVSKE
                     ISSFVQPLGATINMDGTAIYQGVCAIFIARVYGIPLGIPQYLMIILTATLASIGTAGV
                     PGAGMIMLSMVLQTVGLPIDGIMLVAGVDRILDMARTCMNVTGDAAGAVVIAASEGEL
                     VAVPGGSAAAAR"
     gene            3475..5304
                     /locus_tag="PLM74_06595"
     CDS             3475..5304
                     /locus_tag="PLM74_06595"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HON42781.1"
                     /translation="MQRRNICVVTLIALFLLTVAASPAFADCTSVVVGRKASADGSVL
                     LGHNEDNGGRIVMPQYVVPRTTHEPGEVIRFENGGTTPQVPVTWGFIWSQTPGGSFSD
                     FFVNEWGVAVASDSCSPTREDGYDELVARGDITDGGIGYHIRRIVAERATTAREGVEI
                     AAKLVEEFGYIAAGRSYQICDSKEGWLMHIVRGRHYVAQRVPDDQVVIIPNCYVIREV
                     DLNDKENFIASPDLIDYAIARGWYDPSSGKPFDFAAAYNTSPSGKSLERGYDTRQWIG
                     QKLITGVTPSTTPLPFAVTPAKKMTVADVMDLLSNHYEDTQYDKTNGYQTSPHWTDER
                     VICTSSTQESSVTVLRDGVPDPIKAVYWRTNGRPCTNPYIPWYLGMTSVPEGYSWMEP
                     NLGRSIQFAPHNALYDYDSTKAWWVFQDLQNAVDGQYGKAIGRVQQVWKEFQDEVFAE
                     QPAVDAIVTRLAAVDEQYAREFVTQYTNSLTSRAAAIAKELTRELVTADLHVEAKAVR
                     LAGEGVFTASLLSSETFDAGKINYADVAIGPGYRNPKTWVPANSGKLVDVNGDGRLDL
                     VMEFSIPAMAKIVGVACYTDVWLRAVTDSGEPVVAKGLVDFVK"
     gene            5412..6236
                     /locus_tag="PLM74_06600"
     CDS             5412..6236
                     /locus_tag="PLM74_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42782.1"
                     /translation="MATSLHRKTAVWAILAIVLATAHISVGWAQEGTYTLTPVIESSY
                     KRLAADQAVQSALRFLAHTEPVPGAEGNALFYQSLLLDAGLEHVCVDKAGSVLGTLKG
                     AKMGDTVVVACGVRDVPALLLVIRAFRVSGVRPVVDVAFACSPLVWTMADADASSTES
                     LVRVFDSVGAFISLGREETGAITYPLEGYGSPEAARRGLAVQAAYAAHQALGVDPVAQ
                     GLSSRSIAVPLGIPAIRMGLGNNVLGPQSLFMTIVGIAGVNGVTTPFVTWASDPCT"
BASE COUNT         1356 a         1820 c         2051 g         1384 t
ORIGIN      
        1 gcaggcggtg gggcgcgcag ccgagggaat cgtgaacgag gtccgcaggc agttccgtga
       61 gatcccgggc ctgatggaag gcaaggccag gccggattac gcccggtgcg tcgacatgtg
      121 tgcgggcgct gctcagaagg aaatggtggc gccgtcgctc atagcgataa tcgcgcccat
      181 tctcgtcggt gtgctgctag gtccggacgg cgttggcgga ctgcttgtgg gcacggtggt
      241 aaccgggttc ctgttggccg ttatgatggc caacgccggc ggcgcctggg acaatgccaa
      301 gaagtacatt gaatctgggc agtacggcgg aaagggatct gatgcccaca aggccggagt
      361 ggtaggcgat actgtgggtg atccgttcaa ggacacttca ggcccctcgc tcaacatatt
      421 gatcaagctc atatcgatgg tatccatagt gttctcgtct gtgatcgtcg cattgtcgat
      481 catcaagtag acgctgcaga taccagcgcc tttcctacat tcctcgcgat gcggaccggc
      541 gcccactcat tgccagtggg ggccggttct cgtcacctgc cttgaaaccc aacagccaga
      601 gtcggtcccg acaagattcg aaatttatag caggaaaccg acatactgcg ttgaatggtc
      661 aacttagcga ccgcaaccac agagccgcac ttctagtatt cgtggtccag aaggcagcaa
      721 ctatcattca ctatttaggg gtttagattg tgcacgtgtc gcgaaaacag aaccttctca
      781 tcggtctgtt gttgatgacc gtagtcgtcg cgtgttcagg cgtcggcctg gcagaggagt
      841 acgttctttc ggacagcacg aaggctgtgt acggaaaact catggccact ccggcggtaa
      901 agcagggtat tgagttcctg tttgccgacg atgccaacac cctcgctgag cagatagaga
      961 tctgcgaaat ccccgcgcct ccattcaagg aggaagtccg cgcagcggaa tacatgaagc
     1021 gccttgcggc actcggcctc aaggatgttc agctggacgc cgagggcaat gtattcgggg
     1081 ttcttcccgg aacagggaac ggccccaagc tcttggtctg cgctcacctc gacaccgtgt
     1141 tccctgaggg gaccgatgtt acggtgaagg tgaaggacgg taggtacagc gcccctggta
     1201 tcggcgacga tacccgcggt cttgcggcgc tcctatcggt tattcgcgct ttcaatgaga
     1261 ccggcatcaa gcccgtcggc gacgtcattt tctgcggaaa cgtaggagaa gaggggcttg
     1321 gcgatctcag gggcgtgaag gcgctgttcc gcgatcacaa ggatatcgac gggtttatca
     1381 cggtggacgg agacggagca ggatggatcc tgtatcttgc tacaggcagc cacaggtacg
     1441 agataacctt caacggacct ggcggccata gctttggcgc atttggtctg cctagcgcca
     1501 tccacgccat ggggcgtgcc atcgccaaga tcggcgatct gcagacgcca aaggagccca
     1561 ggacgacctt cacggtgggg gttgtcgagg gcggaacctc gatcaactcc attgcggctc
     1621 aggccaagat gtgggtggac atgaggtcca acgagctgcc tccgctgctt gcaattgaga
     1681 aggagctcct cgctgcggtg aagcaggcag tcgttgagga gaacgccaga tggaacagtg
     1741 acaagatcac tgtcgatatc aagatggtag gggacaggcc tgcgggaacg cagcctgccg
     1801 ataccagcat agtgcaggct atgtatgcgg cgacagtcgc tcttggtata gagccggagc
     1861 ttgagggtcc tgccagtacc gatgctaacc ttcccattag ccttggcatt cccgcactgt
     1921 gtatcggcgg cggaggaagg ggaggcaacg gccacgcgtt cgacgagtgg tacgaaaacg
     1981 ttgatgccta ccttgggacg cagaacatct tcctgacgat actgggcctc gccggagtcg
     2041 acggcgtaac ccagcctctt ctcgaggttc gcgccaagta gctagcgact ctaccccagg
     2101 caggttctcg ctctgtggct tgcggtcaca tcaagctctg ggaatcattg aactagcctc
     2161 atcgggtcaa gagggaggac aactatcgga tgaaactctg ggtgaagatt ctgatcgggt
     2221 tcgcgctggg cgcggttgtc ggcctcattg tggggccttc catagcggtg attaagccgc
     2281 tgggagatct cttcatcagg ctcatcaaga tgctgattgt gccgatggta ttctgctcac
     2341 tggtcgttgg cgcgagcagc ataggcgacc ttaagaaact gggcagggtc ggcggcaaga
     2401 cggtgttgta cttcttggta accaccgcat ttgcaataac tctggcgatc ctgctgtccc
     2461 tgataatcga tccaagcgcg ggatatgtaa tcagcgaagt gggaacgtat gaagcgaagg
     2521 cgatgccgag catcgccact acgttcctta acatggtgcc gactaacccc attaacgccg
     2581 cagcgactga caacatcctt cagatcatcg tgtttgcgct gttcatgggg attgggattt
     2641 cgcttctggg cgagaaaggc aagccggccc tggcattctt cgatagtgtt gcggagatca
     2701 tgtacaagat aactgccata gtgatgggct ttgcgcctat aggcgtattc gcgctgattg
     2761 cgtgggtcac gggcaccagc ggggccgacg tgttgctgcc cctggccaaa ctgatattcg
     2821 tgtgctatct ggccaccgcc ctgcacgcag tcatagtata ctcgggagcg gtgtacatat
     2881 gggcaaagat gaccccgatc acgttcttca aggggttctt cgaagccatg gcggtagcct
     2941 tcagctcgtg ctccagcagc gcgacgctgc ctatctcgat gcggtgcgct caggagaacc
     3001 tgggcgtgtc caaggagatt tccagcttcg ttcagccgct gggcgccacc ataaacatgg
     3061 acggcacagc catttaccag ggcgtatgcg ccatattcat cgcgcgggtg tacggtattc
     3121 ctctaggtat tccccagtat ctgatgatca tcctcacagc cactctggcg tctatcggaa
     3181 cagccggtgt gccgggcgca gggatgatca tgttgagcat ggttctgcag acggttggac
     3241 tgccgataga cgggatcatg ttggttgcag gtgtggatcg tatacttgac atggccagga
     3301 cctgcatgaa cgtaacaggc gacgctgccg gtgccgtggt catcgcggcg tccgaaggcg
     3361 aactagtcgc agttccagga ggcagcgcgg cggccgccag atagctactc gaatcggcgg
     3421 tgttcggccc atcgggccgg aacctaagtg agagggaagg aagcgtttat aacaatgcag
     3481 cgtaggaata tctgtgttgt aaccctaatt gccctgtttc tcttgaccgt tgctgcatcg
     3541 ccggctttcg ctgactgcac aagcgttgtt gtcggtcgca aggcatcggc cgatggctcg
     3601 gttctcctgg gccataacga ggataacggc ggacggatcg tcatgccgca gtatgtcgtg
     3661 cctcgtacga cacacgagcc tggcgaggtc atccggtttg agaacggcgg aactactcca
     3721 caagtgccgg taacctgggg attcatctgg tcgcagaccc ctggcggttc cttcagcgac
     3781 ttctttgtga acgagtgggg cgttgccgtg gccagcgata gctgcagccc cacgcgggaa
     3841 gacggctacg atgagctagt ggccaggggc gacatcaccg acggcggaat cggctaccac
     3901 attcgccgaa tcgtcgccga gcgcgccacg accgcacgcg agggcgtgga gatcgctgct
     3961 aagctagtag aggagttcgg ctatatcgct gccgggcgtt cataccagat ctgcgactcc
     4021 aaggaaggct ggctcatgca catagtccgc ggtaggcact atgtggcgca gagagtaccc
     4081 gacgaccagg tagtcatcat tcccaactgc tacgtgatcc gcgaagttga tctcaatgac
     4141 aaagagaact tcatcgcgtc tcctgatttg attgactacg ctatagcgcg tgggtggtac
     4201 gatccgtcga gcggaaagcc tttcgacttc gcggccgcct acaatacttc tccgagcggc
     4261 aaatcgctgg agcgagggta cgacactagg cagtggatcg gtcagaagct catcaccggc
     4321 gttaccccga gcacgacccc tctcccgttc gccgtcacgc ctgccaagaa gatgacggta
     4381 gcggatgtca tggaccttct gagcaaccac tacgaggaca cacagtacga caagaccaac
     4441 ggataccaga catcgcccca ctggactgac gagcgggtca tatgcacgag ctcaacccag
     4501 gaaagctctg tcaccgtgct gcgtgacggg gtacctgatc cgatcaaggc ggtgtactgg
     4561 cgcactaacg gccggccttg caccaacccg tacatcccct ggtatctcgg gatgacatct
     4621 gtacccgaag gttacagctg gatggagccg aacttgggac ggtccattca gtttgcgccc
     4681 cacaacgcgc tgtatgacta cgattccacc aaggcatggt gggtgttcca ggatctgcag
     4741 aatgcggtag acgggcaata tggtaaggcc atcggccgag ttcagcaggt atggaaggaa
     4801 ttccaggacg aggtattcgc agagcagcct gcagtggacg caatagtcac ccgtctggct
     4861 gctgttgatg agcagtacgc cagggagttc gtcacgcagt acaccaacag ccttaccagt
     4921 agggctgctg ctattgccaa ggagctcact cgcgaactcg tcaccgccga tctgcacgta
     4981 gaagctaagg cggttcgcct ggccggcgag ggcgtgttca ctgcaagcct gctaagctct
     5041 gaaacgttcg acgcgggcaa gatcaactac gctgatgtcg ccataggacc tggttaccgg
     5101 aacccgaaaa cctgggtacc tgcgaatagc gggaagctag ttgacgtgaa cggcgatggc
     5161 cggctcgatc tcgtgatgga gttctccata ccggcgatgg ccaagatcgt gggcgttgcc
     5221 tgctacactg atgtgtggct gagggccgtc actgattccg gcgaaccggt agtggcaaag
     5281 gggctagtgg acttcgtaaa ataactacgg tagcggctta actccaaggc gtcggccccg
     5341 tggcaacccg cgtggccggc gccttcgcga tagggcacgc gacctcatct caggcgctgg
     5401 gaggagtgac cttggccact agtcttcata ggaaaactgc tgtctgggcg atactcgcca
     5461 tagtcctggc gactgcgcat atttccgtcg gttgggcgca ggaaggcaca tataccctga
     5521 cccccgtgat cgagtcatcc tacaagcgac tcgcggcgga tcaagccgtt cagagcgcgc
     5581 tgcggtttct ggcgcatacc gagccggtgc cgggcgccga agggaacgcc ctattctatc
     5641 agagccttct tcttgacgcg gggcttgagc atgtctgtgt agacaaggca ggcagtgtgt
     5701 tgggaaccct gaaaggggcc aaaatgggtg atacggtagt cgtcgcgtgc ggcgtcaggg
     5761 acgtcccagc tcttcttctg gtgatccggg cgttcagggt gtcgggcgtg cgccctgtag
     5821 tcgacgtagc gttcgcatgc agtccccttg tatggaccat ggcagacgct gatgcgagca
     5881 gtactgagtc gctggtacga gtgttcgata gtgtcggcgc gttcatctcc ttgggtaggg
     5941 aggagacggg cgccatcact tacccactcg aaggctacgg tagccctgag gccgcccgac
     6001 ggggcctggc ggttcaggct gcttacgccg ctcaccaggc gctgggtgtt gacccggtcg
     6061 cccaggggct atcgagccgg agcatcgctg tgcctttagg catccccgca atccggatgg
     6121 gactaggcaa caacgtactt gggccccaga gcctgttcat gacgatagtc ggtatcgcgg
     6181 gtgtaaacgg cgttaccacg ccctttgtca cgtgggccag cgatccatgc acctgaggtg
     6241 actagcgctg agctgtatgc tcagtatcca gaagccttgt gggattcaag ccgtgtcgga
     6301 tggaagccct gtcggattcc gtgcccgcgt cgtcttgcag caccccacgt cttccagacg
     6361 cgacaggatc ccaatcgcca aagccgagcc gaacagggcc tagcctagct cgttgggaaa
     6421 ccgtacggtg tttccgctag cctgccggaa taccatcgtt acgcgcacct ccgccggttt
     6481 acgctgcgct ggggtgcgtg tgtattgtgg gctggggggg tcatgctcac ttcgctggag
     6541 tggcggttta gctggccttg gcatcatggc cgtcggacaa aacggctgga gctggcatat
     6601 cgtcgatttt t
//
LOCUS       DAOTAC010000127         6593 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_9909_length_6593_cov_3.487305, whole genome shotgun sequence.
ACCESSION   DAOTAC010000127 DAOTAC010000000
VERSION     DAOTAC010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6593
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9909_length_6593_cov_3.487305"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..712)
                     /locus_tag="PLM74_06605"
     CDS             complement(29..712)
                     /locus_tag="PLM74_06605"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HON42783.1"
                     /translation="MPSGDGHSDADIRRHAGLAMYIAKRFSGRAELADLYQAGCVGLV
                     RAMRTYDPTRQTAFTTYAVPLIISEILAYLRGSGPLHIPRPVRELSQKCREAHSALAA
                     ELGSDPNVSQIAARLGVSPADVAFAMDSDAPVRSLDEPAYAASDDLSLLDAVPGEPMP
                     DDDVCDSLALKNALGRLSANARAVILMRFFEERTQAQVARALGISQPQAHRIEKAALR
                     ELRRSMAGE"
     gene            complement(712..1137)
                     /gene="spoIIAB"
                     /locus_tag="PLM74_06610"
     CDS             complement(712..1137)
                     /gene="spoIIAB"
                     /locus_tag="PLM74_06610"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009259275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HON42784.1"
                     /translation="MEPKVNWARVEFESVAENLGFARVAVAGWAAGLGFTLEGLDDIK
                     LAVSEAVSNCIVHAYPQRPGRVLVQLSASDHALTVSVQDEGIGIEDVPAARRPGVSSS
                     SQRMGMGFTLMEALSKSMEVTSSPGHGTTVVMSFAPGDR"
     gene            complement(1122..1490)
                     /locus_tag="PLM74_06615"
     CDS             complement(1122..1490)
                     /locus_tag="PLM74_06615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="This anti-anti-sigma factor, or anti-sigma factor
                     antagonist, belongs to a family that includes
                     characterized members SpoIIAA, RsbV, RsfA, and RsfB;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0045152 - antisigma factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HON42785.1"
                     /translation="MSLFDWVLRGSDLIVDLHGELDLSCSERAAQALDSLIDSNRGAR
                     RVILRMDRVTFIDSSGIGMVFGRHRRLAARGISLAIAGAGVRVTRTLDLLGVSELMPV
                     YASLERALSAQEGVRKWNPR"
     gene            complement(1562..2812)
                     /locus_tag="PLM74_06620"
     CDS             complement(1562..2812)
                     /locus_tag="PLM74_06620"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HON42786.1"
                     /translation="MSYKPAMPVPTPWARALLACAIVLSFHCLPAPAALASPAAPGSP
                     AAAAAAELAKKITAPSAILMEATTGRVLWEKNADVARPIASVVKIMTLTLVMEALEAR
                     RIGLGDMVITSEHVASMGGSQIWLEPGESMAVSDLVKSVAIESANDAAVALAEHVAGS
                     EAAFVAMMNEKARALGCANTRFVNATGLPEPGADSDCVSSARDVAIMSRELLRYPQIH
                     EWLTIWIGYVRDGKNMLTNTNRLVRFYPGADGLKTGYTDRAKYCLSATAVRSNVRVIG
                     VVLGVSTSSVRFAEASTLLDYGFKMCEAQTLARAGQELGRVRVVRGVDTSVNVTVAHD
                     LSVVTLRGEGGKVEARIALPEQLEAPVEQGRPVGRMVAFLDGVEVAQVDVIAAHAVPK
                     APWFQLISRHTREYIRAHFLFTSG"
     gene            complement(2891..4198)
                     /locus_tag="PLM74_06625"
     CDS             complement(2891..4198)
                     /locus_tag="PLM74_06625"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HON42787.1"
                     /translation="MRAPDIIMRKRDGHALSAGEIGYMIQGMLTGEVPDYQISAFCMA
                     VFFQGMTPEETTALTMAMADSGRRVDLAAIRGVKIDKHSTGGVGDTTTLVLTPMVAAA
                     GVPVAKMSGRGLGHTGGTLDKLESIPGFRVSLSEREFVDQVNRIGLAVVGQTEDVAPA
                     DKRLYALRDVTATVESIPLIASSIISKKLATGADAIVLDVKVGSGAFMHELGKARELA
                     LAMVDIGRMAGRRMAALITDMNQPLGQAVGNSLEVIEAIDTLSGRSGGRLLELCFELG
                     ARMVVLGGACDGIKDARVILRRLVESGAALAKLKEMVEAQGGDVSCVDDPGRIEVAPL
                     STLIRAPKDGYVCRADALTLGRAAATMGAGRQRKTDSVDLGCGLRVLVSVGDRVEVGQ
                     PVAELFASAQSRLDEGTIEATSAFAYSDAPCQAPPLIYEAVEA"
     gene            complement(4229..5419)
                     /locus_tag="PLM74_06630"
     CDS             complement(4229..5419)
                     /locus_tag="PLM74_06630"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HON42788.1"
                     /translation="MRAVLVVLDGVGVGELPDAGLYGDRGSNTLVNTARAAGGLSLPN
                     FGRLGLGNLVADAGFELTGVAACDSPVASYGRMAQASPGKDTTTGHWEIAGIRLEKAF
                     PTYPDGFSPEIITTFEEAIGRPVLGNRPASGTEIIKELGEEHMATGSPIVYTSADSVF
                     QIAAHEDVIAPDELYEMCRTARAILTGPHAVGRVIARPFEGSPGHFARTVRRRDFSLS
                     PPGPTLLDLVAEAGLPVMACGKIDDIFAQQGITLSRHVAGNDDVFDAVLQFLDEGRPG
                     LIFANLIDFDMKWGHRNDVEGFARGLEHVDQRLPELLQRLKDDDLLAMCADHGNDPTT
                     PSTDHSREYVPILAASPAAVRAGRSGRPIGVRSSFADLGATIAEFLEIPWSLAGDSFL
                     RHVL"
     gene            complement(5435..6343)
                     /gene="xerD"
                     /locus_tag="PLM74_06635"
     CDS             complement(5435..6343)
                     /gene="xerD"
                     /locus_tag="PLM74_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007934077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HON42789.1"
                     /translation="MLSCIEEYISYLAVERGLSENTLEAYARDLSQFASFVAHLTRGE
                     DEPREEDAIGAGQSLVAAFIAHGMESGMSAASASRRLSALRGFYRFLVREGHIARDPT
                     ANLDTPRQAQRLPRALTGEEVERLLAAPSGSDPRSLRDRAMLELLYASGLRVSELVSL
                     STRDVDLEVGYVRCIGKGDRERIVPMGQAAAWAIQRYLALGRRELASASDDGSLFVTG
                     RGTGMTRQAFWKIIKRKAVEAGITARITPHTLRHSFATHLLEHGADLRSVQEMLGHAD
                     IRTTQVYTHLTQTRLREVYEKHHPRA"
BASE COUNT         1273 a         2150 c         1920 g         1250 t
ORIGIN      
        1 cagctagtat gcgacgcgcc gggagctttc attcaccagc catgctgcgc ctgagctctc
       61 ggagggcagc tttttcaatg cgatgggcct gtggctgcga gatgccaagt gctcttgcca
      121 cttgggcctg tgtacgctcc tcgaagaagc gcattaagat gacagctcgt gcgttagcgc
      181 tcagacggcc caatgcgttt ttcagcgcga gcgaatcgca cacgtcgtcg tcaggcatag
      241 gttcgcctgg tactgcatcg aggagagaca ggtcatcgga tgcggcgtac gcgggttcat
      301 cgagggagcg gaccggcgca tcagagtcca tagcgaacgc aacatcggcc ggagaaacgc
      361 ccagccttgc cgctatctgc gaaacgttcg ggtccgagcc tagttctgca gctagtgcgc
      421 tatgcgcctc ccggcacttc tggctgagtt ctcgcacagg ccgcggtatg tgaagcgggc
      481 cggagcctct gagatatgcc aggatctcgg agattatgag gggaaccgcg taggtcgtga
      541 aagccgtctg gcgcgttggg tcataggtac gcatagctcg gaccaggcca acgcagccag
      601 cctggtacag atctgccagt tccgctcgcc cggagaacct ctttgcgatg tacatggcaa
      661 gcccagcgtg cctacggata tcggcatcgc tatgtccatc gccggaaggc atcagcggtc
      721 acccggtgca aaagacatta ccactgttgt accgtgccct ggcgaagatg tgacttccat
      781 cgatttggac aaggcctcca taagagtgaa acccatgccc atcctctggg acgaactgct
      841 cacacccggg cgacgggctg ccgggacatc ttcgatccca atgccttcgt cctgtaccga
      901 cacggtaagg gcgtgatcgg aggcggatag ctgcaccagc accctgcccg ggcgctgggg
      961 atatgcgtgg actatgcagt tggacacggc ctccgacaca gctaacttga tatcgtcaag
     1021 cccttccagc gtgaacccaa gccccgccgc ccagccggct acggcgactc gcgcgaaccc
     1081 caggttctca gctaccgact cgaactccac acgcgcccag ttcaccttgg gttccatttc
     1141 ctgaccccct cttgcgccga caaggcacgc tctagtgatg catataccgg catcagctcc
     1201 gacacgccga ggagatcgag ggttcgagtc acgcgcacgc ccgcgccggc aatggccagg
     1261 gagatgccgc gcgcggccag gcgtctgtgc ctgccgaaca ccattccaat gcccgagcta
     1321 tcgatgaacg tgactctgtc catcctaagg atcacgcgcc gcgcgccgcg gttggagtcg
     1381 atcagcgagt cgagagcctg cgccgcacgc tcggagcagg acaaatcgag ctcaccatga
     1441 aggtcaacga tgagatctga tccgcggagc acccagtcga acaaggacaa tcccccgcct
     1501 ccctatggat tgcgccggga aacagattct cctttggcgg gggcctatcc ttcttggtac
     1561 gtcagcccga agtaaacagg aagtgcgctc tgatgtactc acgggtgtgc ctcgagatca
     1621 gttggaacca tggtgccttg ggcaccgcat gcgctgcgat tacgtccact tgagccacct
     1681 cgacgccgtc gagaaacgcc accatccggc ccacgggtcg gccctgttcc acaggggcct
     1741 caagctgctc gggcagggcg atcctggctt cgaccttgcc gccttcgccc ctcagcgtca
     1801 ccacgctcag gtcgtgagct actgtgacat tcaccgatgt gtcgacccct cggactactc
     1861 gcacacgccc tagttcctgc cctgcccgag ccagcgtctg ggcctcgcac attttgaagc
     1921 cgtagtcgag cagagtagac gcctccgcaa accgcactga actagtggaa acgcccaaca
     1981 ctactcctat gactcggacg ttgctgcgga ccgccgtggc cgacaggcag tacttggccc
     2041 tgtcagtgta gccggtcttc aagccgtcgg cgcccgggta gaacctgaca aggcggttgg
     2101 tgttcgtcag catgttcttg ccgtcccgga cgtaaccgat ccagatcgtc aaccactcgt
     2161 ggatctgggg gtagcgcaaa agctcccttg acatgatggc cacatcgcgc gcagacgaaa
     2221 cacagtcact atcggccccg ggttccggca gcccggttgc gttgacaaaa cgggtgttgg
     2281 cgcagccgag ggcgcgggcc ttctcgttca tcatcgcgac aaaggcggcc tcgcttcccg
     2341 ccacgtgttc agccagcgcg acggcagcat cgttggccga ctcgatggca acggacttaa
     2401 ctaggtccga cactgccatc gactcgcccg gctccagcca gatctgagaa ccgcccatcg
     2461 aagccacatg ctcggaggtg atcaccatgt cgcccaggcc tatgcgacgc gcttccagtg
     2521 cctccatgac aagtgtcagg gtcatgatct tgaccacgct cgctatgggc cgggcgacgt
     2581 cggcgttttt ctcccacagg accctaccgg tggtggcctc catgaggatg gctgacggcg
     2641 cggttatctt ctttgcaagc tctgccgctg ctgctgcggc aggcgaacca ggcgccgcgg
     2701 gcgacgccaa ggccgccggc gctggcagac agtggaagct cagcacaatg gcacaggcga
     2761 gcagagcacg ggcccagggg gtcggaacgg gcatggcagg tttgtacgac aaggggcatg
     2821 cctcccttcg tctcttgttt caagagagca tattccgtgg catgcccggt tatacaggat
     2881 gagttcgtct ctacgcctcc acggcttcgt agatcagggg cggcgcctgg cacggcgcgt
     2941 cagagtaggc gaaggctgat gtcgcttcga tcgttccttc atccaaccgt gattgggcgc
     3001 tggcaaagag ctccgccacc ggctggccga cctccactct gtcgcccacg gataccagga
     3061 cgcgcagccc gcagccgaga tcgaccgagt ccgtctttcg ctgcctgccg gcgcccatgg
     3121 ttgccgccgc acggcccagc gtaagggcgt ctgcccggca cacgtaaccg tctttcgggg
     3181 cccggatgag ggttgatagc ggcgccacct caatgcggcc cggatcatct acgcatgaca
     3241 cgtcgccgcc ttgagcctcc accatctctt tcagcttggc gagtgcagct cccgattcca
     3301 ccaggcggcg gaggatcact cgggcatcct tgatgccatc gcaggcccct cccagcacga
     3361 ccatgcgcgc gcccagttcg aagcagagct caaggaggcg gccgccgctg cggcccgata
     3421 gcgtgtcaat cgcttcaatg acctcaagcg aattgcctac tgcctgcccc aggggttggt
     3481 tcatgtcagt tatcagcgct gccattctcc gcccggccat acgacctatg tccaccatgg
     3541 ccagggccag ctcacgtgcc ttgccaagct catgcatgaa tgctccgctc ccgactttca
     3601 cgtccagaac gatggcatct gcgcctgtgg ccagcttctt gctgattatg ctggacgcaa
     3661 tcaaaggtat gctctccacc gttgctgtga catcgcgcag ggcgtagagt cgcttgtcgg
     3721 caggcgccac atcttccgtc tgccccacca cagccagacc tatccggttc acctggtcca
     3781 cgaattctcg ttccgagaga gaaactcgaa agcccgggat ggactccagc ttatcgaggg
     3841 ttcctcctgt gtgccccagg cctcggcctg acatcttagc cacaggcact cctgccgcgg
     3901 ccaccatggg cgtgagcacc agggtggtgg tatcgcccac ccctcccgtg ctgtgcttat
     3961 cgattttaac gcctcgtatg gccgcgaggt caacccggcg cccggagtcg gccatggcca
     4021 tagtgagcgc ggtggtctcc tcaggcgtca taccctggaa gaacaccgcc atgcagaatg
     4081 ccgagatctg atagtcaggc acttcccccg tgagcatgcc ctggatcatg tagcctatct
     4141 cgccggcgga aagtgcgtgc ccgtcgcgtt tcctcatgat gatatcgggg gctctcaatc
     4201 caactcctcc tcacgccgtc gggtgtttct acagtacgtg ccgcaagaag ctatcgcctg
     4261 ccagactcca cggtatctca agaaactcgg ctatcgtggc tccgagatcc gcgaagctgg
     4321 acctcacccc gataggccgc ccggagcgcc ccgctcggac agcagcggga ctcgcggcca
     4381 ggatcgggac gtactccctg ctatgatcgg tgcttggcgt agtggggtca ttgccgtggt
     4441 ctgcgcacat ggccaggaga tcatcatcct tgagccgctg taggagctcg gggaggcgtt
     4501 ggtcaacatg ctcgaggcct cgcgcgaaac cttctacatc gttacggtgg ccccacttca
     4561 tatcgaaatc gatcaagttg gcgaaaatca gccccggccg accctcatca agaaactgga
     4621 gaacggcatc gaacacatca tcattacctg ctacatgcct cgacagagta atcccttgct
     4681 gggcgaaaat gtcatctatc ttgccgcacg ccataacggg aagccccgcc tcggccacca
     4741 ggtcaagcag ggtgggacca ggcggggaca gcgagaaatc gcgccgcctg acggttctgg
     4801 caaaatgccc cgggctgccc tcaaacgggc gggctataac ccggcctacg gcgtggggac
     4861 ctgtcaggat cgcgcgggcg gtacggcaca tctcgtatag ctcgtccggg gctatgacgt
     4921 cttcgtgcgc ggcgatctgg aacaccgagt ctgccgacgt atacacgatc gggctgcccg
     4981 tcgccatgtg ctcctcgccc aactccttga tgatctcagt gcctgacgca ggacgattgc
     5041 ccagaaccgg tcggcctatt gcttcttcaa acgttgttat gatctcaggc gaaaacccgt
     5101 ctgggtacgt cggaaacgcc ttctccagcc gaattccggc gatctcccag tggcctgtag
     5161 tagtgtcttt gcctggtgaa gcctgagcca tccgcccata cgaggcgacg ggcgaatcgc
     5221 acgcagcgac ccccgtgagc tcgaaacccg cgtcggccac taggttgccc aggccaagtc
     5281 gcccgaagtt gggcaggctg agcccgccgg cggctctggc ggtattaacc aacgtgttgc
     5341 tgcctcgatc accataaagg cccgcatccg gcagttcgcc cactcccact ccatcaagca
     5401 caaccaagac cgctctcatc gatgtctctc cttatcacgc tctgggatgg tgcttctcgt
     5461 aaacctctct gagcctcgtc tgagtgaggt gcgtatagac ttgcgtggtt cgaatatcgg
     5521 catgtcccaa catctcctgg acagaccgga ggtcggcccc atgctcaagc aggtgcgtgg
     5581 caaatgagtg ccggagtgta tgcggagtga tccgcgccgt gatcccggcc tccactgcct
     5641 tgcgttttat gatcttccag aaagcctggc gagtcatccc ggtcccacgg cctgttacga
     5701 ataggctccc gtcgtcgctt gcggaagcta gttcgcgccg gcccagcgca aggtagcgct
     5761 ggatggccca ggctgcggct tgccccatcg gtactatacg ttcgcgatcg cccttgccta
     5821 tgcagcgcac gtaacccact tccaggtcaa cgtcgcgggt cgacagcgac accagctccg
     5881 agacgcgtag gcccgaggcg tatagcaact caagcatggc acgatcacgc agagaacgcg
     5941 gatcgctgcc cgagggcgcg gccaatagcc tctcgacttc ctcgcctgtc aatgcccggg
     6001 gcaatcgctg tgcctgcctg ggagtgtcga ggttggcagt ggggtcgcgt gcgatgtgcc
     6061 cttcccgcac aagaaaccgg taaaaaccgc gcagcgcgga aagccgcctt gaagcggagg
     6121 ccgcagacat gcccgactcc atgccgtggg cgatgaacgc agcgactagc gattgcccgg
     6181 ctccgatcgc atcttcctcc cgcggttcat cttcaccccg ggtcagatgg gcgacaaacg
     6241 aggcaaactg gctgaggtcg cgggcgtagg cctcgagagt attctctgaa agacctcgct
     6301 ccaccgcaag atacgatatg tactcctcaa tacagctaag caatgactgg agacccccaa
     6361 aaccgcgcgg ttgtcgtgcc catgggcgat caacgcgcat tgccgcccac atgttaatct
     6421 tcgccgcgcg gttccgccct cctgctgctg cgtgggtcat ccaaacctgg cagccagtat
     6481 gaggaaggca ggcgacacat agacttgtac aaacgcggcg aacacgagcg cgacgactga
     6541 caggatgaga gacgcgaacg aacccgtcag ggcacgcgcg acgtccgcat cga
//
LOCUS       DAOTAC010000128         6587 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_9919_length_6587_cov_4.378751, whole genome shotgun sequence.
ACCESSION   DAOTAC010000128 DAOTAC010000000
VERSION     DAOTAC010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6587
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9919_length_6587_cov_4.378751"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1245
                     /locus_tag="PLM74_06640"
     CDS             <1..1245
                     /locus_tag="PLM74_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42790.1"
                     /translation="KAQIGQLSLKLEDAVTAIASARAWAEAAIASGFEAERIAREKDL
                     ADLRAELASLTALQDDMAQSSALALGAAVEAERRERTVADDKLGLSLDQLGGRVTALE
                     ETLTLRISSEISQANEAIQAERAAREKDVAGLAGSVDELTSALSAKADAERSARQEEV
                     RRILADLAGARSEIADLDRKTAEADAAIIAGLETERARIGAVAAELQALTQAVSELEQ
                     YAQTIEGSLKELGKKQGSDYNQQRQALMDLQVALDKLSKTLSDKISANAAEDLRTGAK
                     VVENAMDIRKVVDALNETRQALNANVASTKEAHAAIAEVNTAVEGLNKRLASIELMLQ
                     GSVDELRRRFADDLVAERWEAEAREVKLQSMIEELQARVERLEGQKASQPAGTMSSGA
                     VIGVLAVVAAIVAAIALGGSGK"
     gene            complement(1344..1745)
                     /locus_tag="PLM74_06645"
     CDS             complement(1344..1745)
                     /locus_tag="PLM74_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42791.1"
                     /translation="MKRSLVVLCVAAMVTAAVSVAILAAAPGNPQNGLGTAADVSPNG
                     CTDCHTKSGGVDNSLAAAVKKSASKHVAVKEDINNCYICHAKRADMGKIMHQGHLGEG
                     SSFISIYGGSCTHCHKVDPKTGMVSVKGVKK"
     gene            1936..2115
                     /locus_tag="PLM74_06650"
     CDS             1936..2115
                     /locus_tag="PLM74_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42792.1"
                     /translation="MDEASSRGFIADILVIAGLMASMWLLTEGEEERGRGGQPETVKA
                     VGPPDLHSDKGESQS"
     gene            2150..3316
                     /locus_tag="PLM74_06655"
     CDS             2150..3316
                     /locus_tag="PLM74_06655"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HON42793.1"
                     /translation="MMRSSFRFSESGRPRQQKIVIFDDSVDRADWVGILSGCRGYVVK
                     ELPLVNGLLVLVDEDEATDVQSLASSRSGVLRVDDDIDISMAFEVGRMEWGPRLMENT
                     PWGISRINLTGVNRTGRGVKVGVLDTGVDSGHPDIGRNVKGGMDTISGSASARDDNGH
                     GTHVSGTIAALRNGIGVVGVAPDASVYSVKVLDSRGSGKLSNLVQGMEWCVSQQIKVV
                     NLSLSASKENQTFRDVVLAARKAGITMVCAAGNSGPGPNTVGYPARYIETIAVAAIDE
                     RDSVPNFSSRGREIAVSAPGVDVLSTWPGRRYRKSSGTSMAAPHVTGAVALLLEADPA
                     LTPSEIKSILQSTSVPVRGAGPDEQGAGVIDVAAALARAAKKRTAAVTRVGVST"
     gene            3313..3822
                     /locus_tag="PLM74_06660"
     CDS             3313..3822
                     /locus_tag="PLM74_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42794.1"
                     /translation="MRLPTDSESVALVRALRPFLSERGQSAADDLLGSVNLISVLDSL
                     GDVVRKRRGMGLQDRPLAFLSGLANLDLDPKTVAKAVENMIDIDKGPAASPPDKSKTP
                     RELQGPRPEDITRMLQSVMAKASSDPSFAALLGSLAEEGMRSNMLPKLIETLGAEIPP
                     EDSPPERQG"
     gene            4278..5522
                     /locus_tag="PLM74_06665"
     CDS             4278..5522
                     /locus_tag="PLM74_06665"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HON42795.1"
                     /translation="MNHSAEPGIVIIGLGPAGLMALEGARDHNGDVPVTIVSGEPHYA
                     YFRPRLSHLLGSEILPEQIAVKPRVWYEERGANVLLGRTARAIDIEHRAVVLDDGASL
                     PAQAVVMACGSSPFIPPLPGRDREGVFSLRTAADARAITEACRRARTVCVVGAGPLGL
                     EAAWALAKLDLKVHLLEHGSRLLAKMLDEPASQMLADIVARAGIELHLSASSESVTPH
                     GVRLAGGREVPGEVVLFSTGVRPNPELARLAGISVNRGVVVDAHMRTSEPWIFAAGDV
                     AEFAGAPGGIWPVAMGQGKVAGANAATAGALTEYTAVPPSYTLMVMGTRIFTIGEAQA
                     EEGAGLTTIVSEDAARGIRRKLVLRGSRLVGGLLFGDLAKSDKIRDGVERGIELPVAE
                     LGTASDYSQVMEMLVRALTASA"
     gene            5554..>6587
                     /gene="miaB"
                     /locus_tag="PLM74_06670"
     CDS             5554..>6587
                     /gene="miaB"
                     /locus_tag="PLM74_06670"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HON42796.1"
                     /translation="MHVQHKKAKVVAFGCQMNERDAETISGLLEQIGYRRAGGDQDTD
                     AGARTGANTSANTDIDADVVIFVTCCVRENAENRAIGNLGDMKRLKAQRPGMIIGICG
                     CMVQQEHVRDLVEHRMPWVDLVFGTHNIHRLPELMARVEAGERVIEVWDEAHEVVEGL
                     PASRAFRHKAFVNITYGCNNFCTYCIVPYVRGRERSRRHDDILAEVRELAMSGCREVT
                     LLGQNVNSYGRGLPPDSDGRVWGFADLVRAVNTVDGIVRIRFTTSHPRDFTDEMIEAA
                     ASCNKVCEHFHLPLQAGSDRVLKMMNRGYTARRYLDLVGRIRQAIPGASITTDIMVGF
                     PGETESDFQAT"
BASE COUNT         1367 a         1781 c         2193 g         1246 t
ORIGIN      
        1 aaggcgcaga taggccagct gtcgctgaag ctagaggatg cagtgacggc cattgcatct
       61 gccagggcat gggctgaggc tgcgattgcc tccggattcg aggccgagcg catagctcgt
      121 gaaaaggacc tggccgacct gcgagcggaa cttgcgagcc taacggcact ccaggacgat
      181 atggctcaat cctccgctct ggcccttggc gccgctgtag aggccgaacg gcgcgagcgc
      241 acggtggcag atgacaagct cgggctgtcg ctagaccagc tcggcggacg cgtaaccgcc
      301 ctggaagaga cgcttaccct caggatatcc agcgagatct cgcaggcgaa tgaggcgatc
      361 caggcagagc gggcggccag ggagaaggat gtggctgggc ttgccgggtc tgtcgacgaa
      421 ctgacttccg ccctgtcggc gaaggcagat gctgagcgtt cagccaggca ggaagaggtt
      481 cgaaggatac ttgcggatct ggctggcgcg cgaagcgaga tagccgatct tgaccgaaag
      541 accgcagagg ctgatgcggc cattattgca gggcttgaga ctgagcgggc tcgcatagga
      601 gccgttgccg cagagctcca ggccctgacg caggctgtgt cggagttgga gcaatacgcc
      661 cagacaatcg aaggcagcct gaaagagctc ggcaaaaagc agggttccga ctacaatcag
      721 cagcgtcagg cgctgatgga cctgcaagta gctttggaca agctatcgaa gactttgagc
      781 gacaagatat cggccaacgc cgctgaagat cttcgtaccg gcgccaaggt cgttgagaac
      841 gccatggaca tccgcaaggt tgtggacgcc ctgaacgaaa cgaggcaggc cttgaacgcc
      901 aatgtcgcat cgacgaaaga ggcgcatgca gccatcgccg aggtcaacac ggccgtcgaa
      961 ggtctgaaca agcgtttggc cagcatagag ctgatgcttc agggaagcgt ggacgagcta
     1021 aggagacgct ttgctgatga tctcgtagcc gagagatggg aagctgaggc ccgcgaggtg
     1081 aaactgcagt cgatgatcga agaactgcag gcccgcgtgg aacggcttga agggcagaag
     1141 gcaagccagc cggcgggtac gatgagctcg ggcgccgtaa tcggcgttct ggctgtagtg
     1201 gccgcaatcg tggcggccat agccctgggc ggaagcggga agtagcttct tcggaggcct
     1261 caatcatgtc gcgcgggccg cggcgctcaa cattgcgccg gcggcccgcg gtttgctctg
     1321 gaaccaccat gtgaaccctg ctactacttc ttgacgcctt tcacactgac catgccggtc
     1381 ttgggatcga ccttatggca atgcgtgcag gacccaccat agatactgat aaagctgctg
     1441 ccctcgccta ggtgcccttg gtgcatgatt ttccccatgt cggcacgttt ggcgtggcat
     1501 atgtagcaat tgttgatgtc ctcttttacg gccacatgct ttgacgcgct ctttttgacc
     1561 gctgcggcca ggctgttgtc aactccgccg gatttggtgt ggcaatcggt gcatccattg
     1621 gggcttacat cagccgcagt gccgaggccg ttctgcgggt tgccgggcgc cgcggccagg
     1681 atcgccacag acaccgccgc tgtcaccatg gcggcaacac acagaactac cagtgatctc
     1741 ttcatccaga cccctccttt tgttagctca agccgctcaa ttgtgcccta tgtcacgatt
     1801 atagcatccg gatacgcaac gcagtagtgg ccgacgcgcg catgtaacca atgctgttcc
     1861 aaagtcataa tgtatagcgc tttggggtcg caggacggcg cgtatcgagg ctgcgccgca
     1921 aggaggggtg catggatgga tgaggcgtcg agcagagggt tcatcgctga tatcctggtc
     1981 atcgccggtc tgatggccag tatgtggcta ttgacggagg gcgaggagga gcggggtcga
     2041 ggagggcagc ctgagaccgt aaaggcagtc gggccgcctg acctgcattc cgacaagggc
     2101 gaatcacaga gctagagcat agtgtgtggg agagcgggga ggtggagtga tgatgcggtc
     2161 ttctttcaga ttctccgagt cgggccggcc gcgccaacag aagattgtca tattcgacga
     2221 cagtgtagat cgtgccgact gggtagggat tctgtcgggt tgcaggggtt acgtggtgaa
     2281 agaactgcct ctcgttaatg ggcttctcgt tctggttgac gaagatgagg cgaccgacgt
     2341 acagtcgctg gcgagctcca gatcgggagt gcttcgggta gacgatgaca tcgacatcag
     2401 catggcattt gaggttgggc ggatggaatg ggggccaagg ctcatggaga acactccgtg
     2461 gggcatatcc cgcatcaacc tcacgggagt gaaccgtaca ggtcggggag taaaggtcgg
     2521 tgtgctggac acgggggttg actcgggtca tccggacatc ggccggaacg taaagggtgg
     2581 aatggacaca atcagcggca gcgcgtctgc ccgcgatgac aatgggcatg gcactcatgt
     2641 atcaggcaca atcgccgcac tcagaaacgg aatcggtgtc gtgggcgtcg cgcctgatgc
     2701 gagcgtatac tctgtgaagg tactcgacag cagagggtcg gggaagctgt ccaacctggt
     2761 ccaggggatg gaatggtgcg tcagtcagca gatcaaggta gtaaacctca gccttagtgc
     2821 gagtaaggag aaccagacgt tccgggacgt cgtgctcgcc gccaggaaag cggggattac
     2881 catggtatgc gcggcgggca atagtggacc cgggccaaac accgtaggat atccggccag
     2941 atacatcgag accattgcgg ttgcggccat tgacgaacgg gactctgttc ccaatttctc
     3001 cagcagaggg agggagatag ctgtcagcgc gccgggcgtg gatgtcctct ctacctggcc
     3061 tggtcgcagg taccgcaaga gctcgggcac gtctatggcg gcgccgcacg tcacgggcgc
     3121 cgtcgcgctc ctgctggagg ctgaccccgc gctgacgccg tctgagatca aatcgatact
     3181 gcaatcgacg tccgtacctg tgcgaggagc aggccccgac gaacaggggg cgggcgtcat
     3241 agacgtggcg gcagcactgg ccagagcggc gaaaaagcga actgcggcag ttacgcgggt
     3301 gggggtgtcc acatgaggtt gcccacggat tccgaaagcg tggctctggt ccgggcccta
     3361 cgcccgttcc taagcgaacg cggacaatcg gctgctgacg acttattggg ttctgtgaac
     3421 ctcatatcgg tgttggattc gctgggagat gtagtcagga aaaggagggg catggggctg
     3481 caagatcgtc cgctggcgtt cttgtcaggc ctggccaacc tggatctcga tccaaagacc
     3541 gtggcaaagg cggtggagaa catgatcgat atagacaagg gtccggcggc ctcgccgccc
     3601 gacaagtcca aaacgccgcg cgaattgcag ggcccgcggc cggaggatat caccagaatg
     3661 ctgcagagcg tgatggcgaa agcttcgtcc gatccgagtt tcgcagcgtt actgggctcg
     3721 ctggctgaag aggggatgag gtccaacatg ctccccaagc tcattgagac gctgggcgcg
     3781 gagattcctc cggaggacag cccgcccgag cggcagggat aagatcggag gtgcgggggt
     3841 gaaactcgac aatctgttct catctagcac ggggcttggc aaactactgc cgctcttcat
     3901 cctgcttatc gtgtttctat tcgtagaatg gtggttctag catgcccatg ggggcacggg
     3961 ggtgagatac gatgactgag aaaagcgcag agaccaagaa aacctggttc ttctggggat
     4021 tcccatggtg gtggtggttg gttctcatcg gcatagtgct ggtagtactc ttccttgtgt
     4081 tcaggtagtt cgcgatccgt ctacatgccc cgatcccaac acaaacccgg gcagctcgat
     4141 cagagtcgga gagccgcgcg tacgcgcggc tctaccgatt ttatccagtg ctgctttccg
     4201 cctggccagc ttgggtggta taatacgcgt agagcgctca ggctcagatc gcggtcgttt
     4261 gaggaggcga tccgtctatg aaccattctg cagagccggg tattgtcatc atcgggcttg
     4321 gcccggcggg cctcatggct cttgagggag cgagagacca caacggggat gttcctgtca
     4381 ctattgtgtc gggcgaaccc cactacgcgt acttccgccc caggctttca cacctccttg
     4441 gatcagaaat cctgccggaa cagatagcag tgaaaccgcg agtctggtac gaggagcgcg
     4501 gcgcaaatgt gttgctcggt cgcaccgcgc gagcgatcga catcgagcac cgagcggttg
     4561 tgctcgacga tggagcgagc ctacccgcgc aagccgtcgt tatggcatgc gggagcagcc
     4621 cgttcatacc accgcttccc gggcgcgata gggagggcgt gttctctctg cgcactgcgg
     4681 ccgacgctcg ggccatcacc gaagcgtgca ggcgagcacg cacggtttgc gtggtgggcg
     4741 ccggcccgct cgggctggaa gcggcgtggg ctttggccaa actcgatctc aaagtgcact
     4801 tgctagagca cggctccagg cttctcgcaa agatgctgga cgagcccgca tcgcagatgc
     4861 tggccgatat cgtggcgcgc gcggggatag agcttcacct atcggcctca agcgaatcgg
     4921 ttacgcctca tggtgtgcgg ctcgcaggcg gtcgggaagt gcctggcgag gtcgtgctgt
     4981 tctcaacggg tgttcgcccc aatccggagc tcgcgagatt ggccggaata agtgtcaaca
     5041 ggggagttgt ggtggacgcg catatgcgta cgtcggaacc atggatattc gctgccggcg
     5101 atgtcgccga atttgcggga gcgccgggcg gtatctggcc tgtggccatg ggtcagggta
     5161 aagtggccgg tgccaatgcc gccacagccg gcgcgttgac tgagtatact gctgtgccgc
     5221 cgtcgtacac tctgatggtg atgggcacaa ggatattcac catcggagag gcacaggctg
     5281 aggagggcgc cggcttaacg actatcgttt cggaagacgc ggcccgtgga atccgccgca
     5341 agctggtgtt gcgaggcagt aggctcgtgg gcgggttgct ttttggggat ctggctaaat
     5401 ccgacaagat acgcgacgga gtagaacgcg gcatcgaact gccggtagcc gaactcggga
     5461 cggcatcaga ctattcgcag gtcatggaga tgctcgtccg agcgctcacc gcgagcgcgt
     5521 agacgcggca tgtaacacta tggaggctag gggatgcacg tacagcacaa gaaagctaag
     5581 gtcgttgcct tcggctgtca gatgaacgag cgcgatgcag agaccatctc cggtctgctg
     5641 gagcagatcg ggtaccggcg tgccggaggc gaccaggata cagatgccgg cgccagaact
     5701 ggcgccaaca ctagcgccaa caccgacatc gatgcggacg tcgtcatatt cgtcacatgc
     5761 tgtgtgcgtg agaatgcgga gaaccgcgct atcggcaacc tcggcgacat gaagaggctc
     5821 aaagcgcaac ggccaggcat gataatagga atctgcggct gcatggtgca gcaggagcat
     5881 gtcagggact tggtagagca tcggatgccc tgggtcgacc tggtgttcgg gactcacaac
     5941 atacaccgcc tgccggaact gatggcccgg gtggaggccg gggagcgggt cattgaggtc
     6001 tgggacgaag ctcatgaggt cgttgaggga ttgccggcat cgagagcgtt ccgccacaag
     6061 gcctttgtga acatcaccta cgggtgcaac aacttctgca cctattgcat agtgccatac
     6121 gttaggggaa gggagcgcag ccgacgccat gacgacatcc tggccgaagt gcgcgagctt
     6181 gccatgtcgg gctgccggga agtgactctc cttggccaga atgtgaactc atacgggcgc
     6241 ggcctgccgc ctgatagcga cgggcgcgtc tgggggttcg ccgacctggt tcgtgcagtt
     6301 aataccgtag acgggatcgt ccgcatacgt ttcactacat cacacccgcg cgactttacc
     6361 gatgagatga tagaggcggc cgcctcgtgc aataaggtgt gcgagcattt ccacctgccg
     6421 ctccaggccg gaagcgacag agtgctcaag atgatgaacc ggggctacac tgcccggcgc
     6481 tacctggacc tggtcggccg tatcaggcag gcaatacccg gggcgtccat aaccaccgat
     6541 atcatggtgg gattcccggg cgagacggaa agcgacttcc aggcaac
//
LOCUS       DAOTAC010000129         6578 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_9935_length_6578_cov_5.019776, whole genome shotgun sequence.
ACCESSION   DAOTAC010000129 DAOTAC010000000
VERSION     DAOTAC010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6578
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9935_length_6578_cov_5.019776"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="PLM74_06675"
     CDS             complement(<1..585)
                     /locus_tag="PLM74_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006676040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HON42797.1"
                     /translation="MTGVNGLTGKTRSVRLNPSQLLVMGFGAVILVGSVLLSLPAASA
                     NGEGVRYIDALFTATSATCVTGLVVVDTATTYSLFGQIVVLLLIQVGGLGFMAMATMM
                     ALVLGRRITLRGRLVLQESLNQFTLAGLVRLTRYLFLTTAVVEGAGALILCLRFSALF
                     PVGKSIYYGIFHSVSAFCNAGFDLFGTVTGPFTSL"
     gene            739..815
                     /locus_tag="PLM74_06680"
     tRNA            739..815
                     /locus_tag="PLM74_06680"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:773..775,aa:His,seq:gtg)
     gene            903..979
                     /locus_tag="PLM74_06685"
     tRNA            903..979
                     /locus_tag="PLM74_06685"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:937..939,aa:Arg,seq:tcg)
     gene            1175..2620
                     /gene="tig"
                     /locus_tag="PLM74_06690"
     CDS             1175..2620
                     /gene="tig"
                     /locus_tag="PLM74_06690"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HON42798.1"
                     /translation="MYKASVVGVEDERTTLEITVEEPRVREAVARAVRRVGARVSVPG
                     FRKGKAPRALLERFVSREAIYDEAIQDFAPEAYEAALRETGLEPIDEPEFDGLNDADL
                     ESGGPLVFRVKFAASPEVKLGDYTAIKLDREQREVTDEDVDRTLQNLREQRAEYVPTE
                     RATVETNDLVTVDIDGYVHGELADSFSAHDVEVVVGSGQLIPGLDEKIAGMSIGEPQE
                     FVVNLPETSEDEETVDRDVTFKVSVKDIKAKQLPDLDDDFAKDIGGADTLAGLRDKIT
                     HSLRHRALDDAIMDLSRRALSSLVTTSEVEIPRLMIDREADEELERLQRRLADQGLTW
                     EQYLAAKSKSEDDIREESREAAPGSVKAKLVVDALAKKEGLMPTVDEVSMAIYRMSFD
                     ALGDDKARLRKLMSDPDTRRAATDMLINAKVLAFLGRSCDADPESARCDECASESEPE
                     SESEPESESEPEPKPEAGEPQGNDSEGEAQE"
     gene            2689..3282
                     /gene="clpP"
                     /locus_tag="PLM74_06695"
     CDS             2689..3282
                     /gene="clpP"
                     /locus_tag="PLM74_06695"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HON42799.1"
                     /translation="MTLIPIVVEQTSRGERAYDIYSRLLKDRIVFIGGPIDDSMANLV
                     IAQMLFLQGEDPDKDINLYVNSPGGSVTSGLAIYDTMQYVKPQVSTVCVGLAASMAAV
                     LLAAGAKGKRYALPYSRVMIHQPAGGTSGQATDIEIQAREIMRIKKITHEILARHTGQ
                     PVDRIEQDTERDYYMSAQEAKDYGIIDNIFEGKAGKP"
     gene            3298..4563
                     /gene="clpX"
                     /locus_tag="PLM74_06700"
     CDS             3298..4563
                     /gene="clpX"
                     /locus_tag="PLM74_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007952676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HON42800.1"
                     /translation="MFKYTDDKSQLKCSFCGKAQEQVKKLIAGPGVYICDECIELCNE
                     IIDEETGGPSEVDITEIPKPHEIRAVLDEYVIGQDDAKKTLSVAVYNHYKRINASSRM
                     DDVELQKSNVMLLGPTGCGKTLLAQTLAKILNVPFAIADATSLTEAGYVGEDVENILL
                     KLIQNADYDIERAERGIVYIDEVDKIARKSENPSITRDVSGEGVQQALLKILEGTVAS
                     VPPQGGRKHPHQEFIQLDTTNILFICGGAFEGIDKIIENRIGRKTLGFGAEVRPKVDR
                     KIGDILRQIMPEDLLKYGLIPEFVGRLPVVVPLDALDEEALVRILTEPRSALVKQYER
                     LFELDGVSLEFTRDALVAVAKKAIARGGGARGLRSIMEKLMLDLMYEIPSRSEIQKVV
                     VTREAVDGQGTPTLMLSEKKSARKKEETA"
     gene            4701..6389
                     /gene="lonB"
                     /locus_tag="PLM74_06705"
     CDS             4701..6389
                     /gene="lonB"
                     /locus_tag="PLM74_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease LonB"
                     /protein_id="HON42801.1"
                     /translation="MSSIGVLGLINLFFAVVIGLYFWNLLKAQQGSKSAVGRESRREM
                     DKLREMRAICLTKPLTELTRPKCFEDVIGQQDALLSLRAALCGPNPQHVILYGPPGVG
                     KTAAARVVLEEAKKSSLSPFRPDAVFVEIDANTARFDDRGIADPLIGSVHDPIYQGAG
                     PLGIAGIPQPKPGAVTRAHGGVLFIDEIGELHSIQMNKLLKVLEDRKVFLESAYYSPD
                     DANIPQHIHDIFRNGLPADFRLVGATTRPSRDIPPAIRSRCLEVYFRALAPDEVGVIA
                     SRALGRVNMAFEDGVIDIVKKYAQNGRDAVNMVQLAMGVTVTEGRKTVLRRDMEWVVE
                     SGQYSPRPEKRVGDVPQVGVVNGLAVWGPGMGSVIEVEATAVRAQSGRGSFTVTGVVE
                     EEEQGDGGRVLRRRSMARSSVDNVCTVLRVRFQIDASDYHVHVNFPGGAPVDGPSAGL
                     AMAVAVLSAIRDIPIDNRLTMTGEISVHGRVKPVGGVVAKVAAAASAGAGRVLIPREN
                     WQDRFEQVEGCQVIAVDDIEEALQLVRARPDASAEQPGIAAVPQEVVAARSASP"
BASE COUNT         1421 a         1839 c         2098 g         1220 t
ORIGIN      
        1 aaggctggtg aaaggcccgg tcaccgtgcc gaacaggtcg aacccggcgt tgcaaaaggc
       61 cgagaccgaa tggaatatcc cgtaatatat ggacttgccc actgggaaca gtgccgagaa
      121 gcgtaggcac aggatcaagg caccagcgcc ctccacgacc gcggttgtaa ggaacaagta
      181 cctggtcagc cgcacaagcc ccgccagcgt aaactggttg agcgattcct gcaacaccag
      241 ccggccacgg agtgtgatcc gccttccgag caccaacgcc atcattgtag ccatggccat
      301 gaagccgagc ccgcccacct gaatcagcag aagcaccaca atctgcccga agagcgagta
      361 tgtagtcgcg gtatccacga ctaccaggcc tgtaacgcat gttgcggacg tggcggtgaa
      421 caatgcgtcg atgtagcgca cgccctcgcc gtttgctgac gccgccggca gcgatagcag
      481 tacagagccc actagtatca ccgccccaaa acccatgacg agcaattgag acgggttgag
      541 cctcacgctc cgcgttttgc cggttaatcc attgacgcca gtcatcggat acccttcctt
      601 caatacgtca agtcgccgtg ctcgccttag tcgtgctcgc ctgcgcccga acgcacacaa
      661 tgctatcatt gctccaaatg ggtgtcaatt tgccttgacc gtcacgctaa ggccgtggta
      721 gaatcaaaaa gcagtgtggt ggatgtagct cagttggcta gagcatcaga ttgtggctct
      781 gagggccgtg ggttcaagtc ccatcatcca ccccatacag tttggccccg ggtgtgttcc
      841 ggggcgcttt ttctttacac ccattttcag ttgtgctata ctttgacgcg gcaatggagc
      901 gcgcgcccgt agctcagccg gatagagcga tggacttcga atccataggc cgcaggttcg
      961 agccctgccg ggcgcgccat tatcgttcgc cgctatcggt tgccggcggg cttgcggtgg
     1021 atagcatgca gcgcactatg gctgttttgc cccggttatc gccgtgagag gcgcgtgccg
     1081 gggcatacta tttcgaagcg cattttgcag aggaatcatc tgctgaactg tcgaataggt
     1141 tacccgaagg acgctcgggg gaacgaggtg taccatgtat aaggctagtg tagtcggagt
     1201 tgaagacgaa cgcacaaccc tggagataac cgttgaagag ccgcgtgttc gcgaggctgt
     1261 ggcacgggcc gtgcgccggg tcggggcgcg ggtttccgta cccgggttcc gcaagggcaa
     1321 agcccctcgc gcgttgctcg agcgattcgt gagccgggag gccatatacg acgaggctat
     1381 ccaggatttt gctcccgagg cgtacgaagc ggctcttcgc gaaacgggtc tcgaacccat
     1441 cgatgagccg gagtttgatg ggctcaacga tgctgacctg gaaagcggcg ggccactggt
     1501 gttccgggtg aagtttgcag ctagcccaga agtgaagttg ggcgactata ccgccatcaa
     1561 gctcgatcgc gaacagcgtg aagtcaccga cgaagacgtg gatcggacgc tccagaacct
     1621 gcgcgagcag agggctgaat acgtaccgac tgagcgcgcg acggtggaga cgaacgatct
     1681 ggtcactgtc gatattgatg gatatgtaca cggcgagctt gcggactctt tctccgcaca
     1741 cgacgttgaa gtcgtggtgg ggtccggtca gctgattcca gggctcgacg agaagatcgc
     1801 aggcatgagc ataggtgaac cgcaggagtt cgtggttaac ctacccgaaa cctcggaaga
     1861 cgaggaaacg gtcgaccgtg atgtgacgtt caaggtatca gtcaaggata tcaaggccaa
     1921 gcagcttccc gatctcgacg acgatttcgc caaagatatc ggcggtgccg ataccctggc
     1981 cgggttgcgc gacaagataa cgcattcact gcgccatcgg gcgctggatg atgcgatcat
     2041 ggatctttca aggcgtgctc tctcgtctct ggtgacgaca tccgaggttg agatccccag
     2101 gctgatgatc gaccgggagg ccgatgagga gctggagaga ttgcagcgga ggctggccga
     2161 tcagggcctt acatgggagc agtatcttgc ggctaagagc aagtcggaag atgatattcg
     2221 cgaagagtct cgcgaggctg cgccgggatc ggtcaaggcc aagcttgtag tggatgctct
     2281 ggccaagaag gaagggctca tgcccaccgt cgatgaggtg agcatggcga tataccgcat
     2341 gagcttcgat gcgctgggtg atgacaaggc caggctgcgc aagctgatgt cggacccaga
     2401 tacgcgccgc gcggcaaccg acatgctaat caatgcgaaa gtcctggcgt tcctgggccg
     2461 atcatgcgac gctgaccccg agagcgcccg atgcgacgag tgcgcgtccg agtcggagcc
     2521 cgagtccgag tcggagcccg agtccgagtc cgaacccgag cccaagcccg aggctggcga
     2581 gcctcagggg aatgattctg agggagaagc acaagaataa cgtggaccag ggcacgggcg
     2641 acggaacacg cggatctgaa tgaatgctac ttgcgggagg tgacggggat gaccttgata
     2701 cccatagtcg tcgaacagac cagtcgcggt gagcgggcct acgatattta ctccaggctt
     2761 ctcaaagaca ggatcgtctt cattggaggc ccgatcgacg acagcatggc gaacctggtc
     2821 attgcgcaga tgctgtttct ccagggcgag gaccctgaca aggacatcaa cctttacgtc
     2881 aatagcccgg gcgggtctgt gacaagcggg cttgcgatat acgatacgat gcagtacgtg
     2941 aagcctcagg tttccacggt gtgtgttggc cttgctgcca gcatggcagc ggtgttgctg
     3001 gcggccggcg cgaaaggcaa gcgttatgcc ctgccgtact cgcgcgtcat gatacaccag
     3061 ccggccggcg gcacgtccgg tcaggccact gacatcgaga tccaggcgag agagatcatg
     3121 cgaatcaaga agatcaccca cgagattctt gcgcgccata cagggcagcc tgttgatcgt
     3181 atcgagcagg acaccgagcg cgactactac atgtccgccc aggaggccaa ggactacggc
     3241 atcatcgaca acatcttcga gggcaaggcc ggcaagccct agccgagagg tggtcacatg
     3301 ttcaagtata ccgacgacaa aagccagctc aagtgctcgt tctgcggcaa ggcccaggag
     3361 caggtgaaaa agctcatcgc aggtcccgga gtgtatatct gcgatgagtg cattgagctg
     3421 tgcaacgaga taattgatga ggagacgggg ggcccgtccg aggttgatat cacggagatc
     3481 cccaaacccc atgagatcag ggccgttctt gacgagtacg tgatcgggca ggacgacgcc
     3541 aaaaagaccc tgtctgtggc ggtatacaac cactacaaga ggatcaacgc gagctcgcgc
     3601 atggacgacg ttgagctgca gaaaagcaat gtcatgttgc ttggccccac cgggtgcggc
     3661 aagacgcttc tggcccagac gctggccaag atcctgaatg tgcctttcgc catagccgac
     3721 gccacatcgc tcacggaagc cggctacgtc ggtgaggacg tggagaacat actgctcaag
     3781 ctcattcaga acgccgacta cgatattgag cgtgctgaac gaggcatcgt ctacatcgat
     3841 gaagtcgaca agatcgcccg caagtcggag aacccgtcga tcacccgcga cgtctcaggc
     3901 gaaggggtgc agcaggcgct gctcaagatc ttggagggca ctgttgccag cgttccgccg
     3961 cagggcgggc ggaagcaccc gcaccaggag ttcatacagc tcgacactac gaacatcctg
     4021 ttcatctgcg gcggcgcgtt cgaggggatc gacaagatca tcgagaaccg catcggccgc
     4081 aagacgttgg ggttcggagc cgaagtcagg ccgaaggtcg accggaagat cggcgatatc
     4141 ctgcggcaga taatgccgga ggatctgctg aagtatggtt tgatccccga gttcgtgggc
     4201 aggcttccgg ttgtggtccc gctcgatgct ctggacgaag aggccctggt gcgcattctc
     4261 accgaaccca ggagtgctct ggtcaagcag tatgaacgcc tcttcgagct ggacggagtg
     4321 agcctcgagt tcacccgcga tgccttggtg gccgtggcca agaaggctat cgcgcgcggc
     4381 ggcggtgccc gtgggctcag gtcgatcatg gagaagctca tgctggacct gatgtacgag
     4441 attcccagtc gcagcgagat acagaaggtc gtggtcaccc gcgaagctgt ggacgggcag
     4501 ggtacaccca ctctgatgct gtcggagaag aagtctgccc ggaaaaagga agaaacagcc
     4561 taacccaagc tctttgctga gctgattccc gttgtcatat cccggcgagc gcctatgcct
     4621 cgccgggata gttgcttctc cccagggcat gatacaccct gagcagcgcg gatgggcggc
     4681 gcggacagga gggagcggcg gtgagcagca ttggcgtgct agggttgatc aacctgtttt
     4741 ttgcggtcgt gatcggtctc tacttctgga acctcctcaa agcgcagcaa ggctcgaaga
     4801 gcgcagtggg gcgcgagtcc cgccgggaga tggacaagct gcgcgagatg cgcgctatct
     4861 gcctgactaa acctctcacc gaattgacca ggcccaaatg cttcgaagat gtcatcgggc
     4921 agcaggacgc gctgctctcc ctacgcgctg ccttgtgcgg gcccaacccg caacatgtga
     4981 tcctatatgg tccccccggc gtcggcaaga ctgccgccgc gcgcgtcgtc ctcgaggagg
     5041 ccaagaagag cagcctttcg ccgttcaggc cggacgcggt atttgtggag atagacgcca
     5101 acaccgcgcg tttcgacgat aggggaatag ccgatccgct catcggatcg gtgcacgacc
     5161 caatatacca gggcgccgga cctctgggta tcgctgggat tccgcagccc aagccgggcg
     5221 cggtgacgcg cgcccacggc ggcgtgttgt tcatcgacga gatcggcgaa ctgcattcga
     5281 tacagatgaa caagctcctg aaagtcctgg aagaccgcaa ggtgttcctc gagtccgcct
     5341 actacagccc ggacgatgcc aacatacccc aacacataca tgacatattc cgaaacggac
     5401 tgccggccga cttccgccta gtgggcgcca ctacacgtcc gagccgcgac attcctccgg
     5461 ccatcaggtc cagatgtctg gaggtatact tccgcgcgct tgcgcccgat gaggtaggtg
     5521 taattgcctc gcgcgccctg ggcagggtga acatggcctt cgaagacggt gtgatcgata
     5581 tcgttaagaa atatgcgcag aatggccggg atgcagtcaa catggttcag ctggccatgg
     5641 gcgtgaccgt aaccgagggg cggaagaccg tgcttcgccg ggatatggag tgggttgtgg
     5701 aaagcgggca atactcgccg cgtcctgaga agcgcgtggg cgatgtgccg caggtaggag
     5761 tggtgaacgg cctcgctgtg tggggtcccg gaatgggctc ggttatcgaa gtggaagcga
     5821 ccgccgttcg cgcgcagtca gggcggggtt ctttcacggt taccggagtc gtggaagagg
     5881 aggagcaggg agacggaggg cgcgtgctgc gccggcgcag catggcgcgg agttcggtag
     5941 ataatgtatg cacggtcctt agggtcagat tccagatcga tgcttccgac taccacgtcc
     6001 acgttaactt ccccggaggc gcgcctgtcg acgggccctc tgctgggctg gccatggcag
     6061 ttgcagtcct gtccgcaatc cgggacatcc ccatcgacaa caggctcacg atgaccggcg
     6121 agatctcggt tcacggccgg gtgaagccgg tgggaggtgt ggtcgccaaa gttgcggctg
     6181 ccgccagcgc cggggcgggc agggtgctca ttcccaggga gaactggcaa gacagattcg
     6241 aacaggtgga ggggtgtcag gtaatcgccg tggacgatat cgaagaggcg ctccagctag
     6301 tcagagcccg gcctgacgct tccgccgaac agcccggcat cgcagccgtg ccgcaggagg
     6361 tggtcgcggc cagatccgct tcgccgtaaa gcggctcttt ccggcgagat ccgagacggc
     6421 gcctcgaatt gaggcgtcat ttttctgtgc ccagctctgc catggtttgc ttgctccgag
     6481 gctcttttct tgaaggaatg caccgagtca aggagaatag gtaagctgca ggcaggaggt
     6541 gcagaaaccc gtggccagag cggaaaacgg attatcta
//
LOCUS       DAOTAC010000130         6577 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_9941_length_6577_cov_4.691046, whole genome shotgun sequence.
ACCESSION   DAOTAC010000130 DAOTAC010000000
VERSION     DAOTAC010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6577
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9941_length_6577_cov_4.691046"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..594)
                     /locus_tag="PLM74_06710"
     CDS             complement(145..594)
                     /locus_tag="PLM74_06710"
                     /inference="COORDINATES: protein motif:HMM:NF027249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system HicB family antitoxin"
                     /protein_id="HON42802.1"
                     /translation="MSSINSLDFRGYGIRVFPVSPENGGGWAAIAPELPGCSACGDSP
                     SEAMDELQDAIDSWIQATREFGDCVPEPKQWAPDKFSGKLTVRLPKSLHRELAECASE
                     QGVSINSLMVSMISRGLAQLSQPGSGTDAGVHGSHSGHAKRVLRVYR"
     gene            complement(581..925)
                     /locus_tag="PLM74_06715"
     CDS             complement(581..925)
                     /locus_tag="PLM74_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42803.1"
                     /translation="MNVPSLVVACPEGDRMPSWRRLFRRLMEGSSDFAPDELLLVAAR
                     LGCDIRQGGKASHIVISHELLRRPGRPVPRIVIVTSRKRAKPYEIARMREFISLVEEA
                     RFMQEAEEGEFH"
     gene            1217..1936
                     /locus_tag="PLM74_06720"
                     /pseudo
     CDS             1217..1936
                     /locus_tag="PLM74_06720"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            2048..2302
                     /locus_tag="PLM74_06725"
     CDS             2048..2302
                     /locus_tag="PLM74_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42804.1"
                     /translation="MSIVWSMWDGYWKEDKYIRPLCETHGVEPVHLHISGDCTWYDIR
                     RLVAGLRPGRIIPIHTEHAEHFARYLQGVTLLQDGEALEL"
     gene            2629..3936
                     /locus_tag="PLM74_06730"
     CDS             2629..3936
                     /locus_tag="PLM74_06730"
                     /inference="COORDINATES: protein motif:HMM:TIGR01918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:3673..3675,aa:Sec)
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HON42805.1"
                     /translation="MRKRVAHYINQFYGGIGGEEAAGAPLAIKDGPIGPGTALQRELG
                     EGYEIVMTIVCGDNYYAENSDYVADAVCHAVSGKSIDLLVAGPAFNAGRYGLACGGVC
                     AAVARRQSIPAVTAMYPENPAVEMYKQDLLIVATGASAAGMRDAIRRVARLVKKMATG
                     EAILSAKEEGYIPRGRRLNQLTERPAAARAVEMLHKKLKGEPYTTEVPVGRYEKVTPA
                     KPITNLAQATIALVTEAGIVPFGNPDRIKHANATSWASCSLEGIDSLEKGQYEAIHGG
                     FDASFANEDPNRVLPVDAMRFFEAEGYIGKLYDTYYVTVGNGTPVERSARFGQEIAQE
                     LLRAGVHGVLIPSSUATGTRCGATLAKEIERVGIPSACITAMSSIAEAVGASRIVIGG
                     RIPYMCSDASLPREREIEYRRKLVKAALDSLCTEVERPTLFSP"
     gene            3968..5257
                     /locus_tag="PLM74_06735"
     CDS             3968..5257
                     /locus_tag="PLM74_06735"
                     /inference="COORDINATES: protein motif:HMM:NF020896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HON42806.1"
                     /translation="MKLNLGTFKVADVVLGRGTSFAGGVLTIDEQEIQDLVTQDGNLA
                     GVQVHIVKPGEPKRIIHVLDVIEPRIKLDTGLDVFPGFLSAPVTAGRGTTYRLSGVTV
                     VESAQLPWDAGGLLIPREGIIDMVGPGRHYSPFHETINVALCFELAEGKSDVEYDASV
                     RMAGIKVSKYLANTVRHLTPDTTEVFSTEGRRSDIPNIVYIHQYQSQGTYAHTYCYGK
                     HMYENLPTVINPNEMIDGALVSGNYAYACFKTPTYLHCNNPVMLELYKGHGINHNFAG
                     VIIARGHNYTFDEKLRSAQYAAKLAKELDAQGAVITWEGGGNAVIEAMQTVKACEELG
                     IKTVIIAYEMGGPEGDDVALLDSVEEADAIVSTGSIEKKITLPPLTPVGGRELRLARE
                     LGGIGVDASGGLEFDVSHEMYCGANQTGFGLITAEDY"
     gene            5292..>6577
                     /locus_tag="PLM74_06740"
     CDS             5292..>6577
                     /locus_tag="PLM74_06740"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004607041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HON42807.1"
                     /translation="MNPYDYVKKEPYKLYINGEFVPSVSTRTFDITNPANNEVFAKAY
                     SGGRADVEKAISAARNAFDHGPWGKMTNRQRSRLLFEARDLLSRRSEEFAALETLDCG
                     KIFTGALHYDVAQALDGFEYYAGKARCLEGNVIPVDGGGRYLNYVMWQPYGVVGEILP
                     WNCPLLMGAAKIASILAAGNTVIIKPSSWASLSMLTMAEVLHEAGFPPGVVNIVTGSG
                     SEVGTALVESPLVDMVSMTGGTDTGRKVISDSSKTIKDVALELGGKSPNIVFPDIDWE
                     SAVKWTLLGFTLGSGQVCVSGTRLILHRSIYDRFLAQLKAECEKLIPGDGFDCEREVN
                     LPSLIHPDHAARVWSYIEKGKAEGARLITGGVPYDDPALAKGNFVPPTVFADVTPEMT
                     IFREEIFGPVLAVTPFETEQQAIDLANDTPFGLAGAV"
BASE COUNT         1476 a         1882 c         1963 g         1256 t
ORIGIN      
        1 gtgccaagcg gtcatcatgt tcggcatcaa caaccgcctc gatgtcgtga gactggaatg
       61 gctgctggaa gaagggcacg tcgattcgct ggagtgagct tgtaggagac agcctgccag
      121 ctgaacctga gagaactcga ggcgctagcg atacactcgg agcaccctct tggcgtgccc
      181 tgagtggctg ccatgtaccc ccgcatcggt tccgctgccg ggctgtgaca gttgggccag
      241 ccccctggag atcatgctga ccatcaagct gttgatgctg acaccctgct cggacgcaca
      301 ttcggccagc tccctgtgga gtgatttagg caatcgaacc gtgagcttgc cgctgaattt
      361 gtctggcgcc cattgcttgg gctcaggcac gcagtcgcca aactcgcggg tggcctgtat
      421 ccacgagtca atcgcgtcct ggagttcgtc catcgcttcg ctaggcgaat caccgcacgc
      481 cgagcacccc ggtagctcgg gagcaatcgc agcccagccg cctccgttct ccggcgaaac
      541 cggaaacacc ctaatcccat agcctctgaa gtccaggctg ttaatggaac tcaccttcct
      601 ctgcctcttg catgaagcgg gcctcctcga caagcgatat gaactcccgc atgcgtgcaa
      661 tctcatacgg tttcgcccgc ttcctgctcg tcactatgac gattctcgga accggcctgc
      721 ccgggcgcct gaggagttca tgggatatca ctatgtgact ggcttttccc ccctgccgta
      781 tatcgcatcc gagccttgct gcgactagga gtagctcgtc cggagcgaaa tcgctgcttc
      841 cctccatcag gcgcctaaag agcctgcgcc aagacggcat tctgtcaccc tccgggcatg
      901 ccaccaccag tgatggcacg ttcattctac gctactcctg cgcggttgtc cagacccagg
      961 agcagaacgc actgccaacc caatgctaga acgagctgaa cagcctgtgt atcgcccgat
     1021 agggtgatcc tggcccgtgc accgtagtca cgtatgccga ctccattgac catgccctag
     1081 cggataccag gatcgaagtg gacgccagct tcgcgaggat ggagcggcag tccggccccg
     1141 acggtgtcga cctcgccctg ccggatcttg tctccggtca cctgcagttg acggcgccta
     1201 tcgcgggcac gagccgagca ttgcggcggt gatcctgtcc cacgcccatc aggaccacac
     1261 gggcctggcc ggctacgtac atgagtccat ccccaaatac gccacccggg gcacatgggc
     1321 cctgcatgac tcgcttgcgg tattcctgcc gcagttggtc tccatccaga accgacgcac
     1381 catacgtgtg ggcaagcccg tgcgaatcgg gccattcacc atcactgccc atctggtcga
     1441 tcactctgcc cccagcgcag tggcccttga agtagaggcc gacgggcaca ggctgttgta
     1501 ttcaggcgac atcagatctc acgggagaga aggcggacta tggaacaagc tcgtcgacga
     1561 gcttgccggc aaggtagaca cgctactgat ggagggcacc accgtcgggc ggcctcaagg
     1621 gccgcgcagg acggaagccc accttgagct ggaattcgtt gagctaatgc gcagccagcg
     1681 ccacatggcc gtggtgttct gctcatcgca caacctcgat agaatcgtca ccatataccg
     1741 tgcggcgaag aggaccggaa agaccatggt gatcgaccta cataccgcat ataccctgat
     1801 ggctctgagg cccatctcga aggcaatacc ccaatggaac cgggacgaga taagggtcgt
     1861 gtcgtgggac tatcaccagg acaggctgcg aagagccggg aggccggatt ttctagagca
     1921 gaccaggccc aaatgagtgg agtgggacga tatgaaagcc actccccgaa agattgtgtt
     1981 gctggccaag tccaaccgct atacgcccgg cgaactcgag gactagtgcg gaagcgctat
     2041 acgggaaatg agcatcgtct ggtccatgtg ggacggatac tggaaggaag acaagtacat
     2101 acgacccctg tgcgaaacac acggggtgga acccgttcac ctgcacatca gcggcgattg
     2161 cacctggtac gacatcaggc gcctggtggc cggcctgcgt cccggcagga tcatccccat
     2221 ccataccgaa catgccgagc acttcgcccg atatcttcag ggcgttacgc tgcttcagga
     2281 cggagaggcg ctcgagctct gagatctgat atcgagtcat ggcgcgccga tcagggccgg
     2341 tcgggggcgc cctcgcccgc acgttgacgc gccgagtggt gcgaacccgc caagtagtct
     2401 tggtcgtgtc agtccactct tccacacgct tcgaaagaaa ttttcatgtc tcagaaggaa
     2461 tttccgaact cgttaccgaa ttgggtctac acctagtatc tagcgagtct tctcagcatg
     2521 gcgctgggct tcatgatggt attctggcac gttgccttgc acgcagtgta atcgggattt
     2581 gggggacgga actgcctgtt caggacgtgt agcggaggtg gaacgtgtat gaggaaacga
     2641 gtcgctcact acatcaacca gttctacggc ggaattggcg gtgaggaagc cgcgggcgct
     2701 ccgctcgcga tcaaggacgg gcccattggg ccaggcacag cgctccagag ggagctgggc
     2761 gagggctacg aaatcgtaat gaccatcgtc tgcggggaca actactacgc cgagaacagc
     2821 gactacgttg ctgacgcagt atgccatgcg gtcagcggga agagcataga tctgctggta
     2881 gcagggccgg ccttcaatgc gggacgctac gggctggcct gcggcggggt ctgcgccgct
     2941 gtggcgcgtc gccagagcat tcccgcggtt acagccatgt acccggagaa ccctgcagtg
     3001 gaaatgtaca agcaagacct gctgatcgtg gctacagggg cttcggcggc cgggatgaga
     3061 gacgccattc gccgtgtggc gaggttggta aagaagatgg ccaccggcga ggcgatcctg
     3121 tcggccaagg aggaaggcta catcccccgc ggcagacggc tcaaccagtt aacggaaagg
     3181 cctgcagccg ctagagcagt ggagatgcta cacaagaagc tcaagggcga gccctacacg
     3241 accgaagtgc cggtgggaag atacgagaag gtcactcccg ctaagccgat cacgaatctc
     3301 gctcaagcaa ccatcgccct agtgaccgaa gcgggcattg tcccctttgg gaatcctgac
     3361 agaatcaagc atgccaacgc aaccagctgg gcgtcgtgtt cgctagaggg catcgacagt
     3421 ctggagaagg ggcagtatga agccattcac ggagggttcg acgcaagttt cgccaacgag
     3481 gacccgaaca gagtcctgcc tgtggatgcc atgaggttct tcgaagccga agggtacatc
     3541 ggtaagcttt atgatacata ttacgtcact gtgggcaacg gcaccccggt cgaaagaagc
     3601 gcaagattcg gccaggaaat cgcccaggaa ctgctgagag caggggtgca cggcgtccta
     3661 atcccgtcca gctgagcgac tggcacgcgt tgcggtgcaa cgctagcgaa agaaattgag
     3721 agggtcggga tcccttcggc ctgtattacg gcaatgagct cgattgcaga ggcggttgga
     3781 gcaagcagaa tcgttatcgg cggtcgcatt ccctacatgt gcagcgacgc ctctcttccc
     3841 cgggagcggg agatcgagta tagaaggaag cttgtcaaag ccgcactcga ttccctgtgt
     3901 accgaggttg agagaccgac attgttctct ccgtgaaacg gctgaggatt gagaggggac
     3961 aaacctcatg aagctgaact taggcacttt caaagtcgcg gacgttgtgt tgggccgtgg
     4021 aacctcattc gccggcggag tgctcaccat cgatgagcag gagatccaag acctggtgac
     4081 gcaagatggc aacctagccg gcgtgcaggt tcacatcgtc aagcccggag aaccgaagag
     4141 gatcatccat gtccttgacg tcatcgaacc gcggataaag ctcgacaccg ggcttgatgt
     4201 ctttccggga tttctgtctg ctcctgttac ggctgggaga ggcacaacat acaggctgag
     4261 tggagtcaca gtggtggaga gtgcacaatt gccctgggac gccggcggac tactaatacc
     4321 acgcgaaggg atcatcgaca tggtgggacc cggcagacac tacagcccgt tccacgagac
     4381 catcaatgtt gctctctgct ttgagctggc tgaaggcaaa tcggacgttg agtatgacgc
     4441 ctctgtccga atggctggca tcaaggtgtc caaatacctg gccaacacgg ttcggcacct
     4501 cacacctgac accacagaag tcttcagcac cgaaggcagg cggagcgata ttcccaatat
     4561 agtctacatc caccagtatc aatcacaagg aacgtatgcc catacatact gctacggcaa
     4621 gcacatgtat gagaacttgc ccacggtaat caacccaaat gagatgattg acggcgccct
     4681 ggtcagcggc aactacgcct acgcatgttt caagacccca acctatctgc actgcaacaa
     4741 ccctgtcatg ctggaactgt ataaggggca tgggatcaac cacaatttcg ccggggtcat
     4801 cattgcgcgg gggcacaact acacattcga cgagaaactg cgctccgcgc aatacgcagc
     4861 aaagctggcc aaagaactcg acgctcaggg cgctgtcata acatgggaag gtggcggcaa
     4921 cgcggttatc gaggcgatgc agacggtcaa ggcatgcgag gagcttggaa tcaagacggt
     4981 gatcatagcc tacgagatgg gcgggcccga gggggatgat gttgccttgc tggacagtgt
     5041 ggaggaggcc gatgccatag tgagcacggg cagcattgag aagaagataa cccttccgcc
     5101 gctcacgccg gtcggaggca gagaactcag actagcaaga gaactaggcg gaatcggagt
     5161 ggacgcgtcc gggggcctgg aattcgatgt ttcccatgag atgtattgcg gcgccaacca
     5221 gacaggcttt gggctgatca ccgcggagga ttactagttc cggactgcgg ctttagaggg
     5281 ggacacagac gatgaacccg tatgactacg taaagaagga accctacaag ctctacatca
     5341 atggcgaatt cgtaccgtcc gtatcaacac ggactttcga tatcactaac cctgccaata
     5401 acgaggtgtt cgccaaagcc tacagcggcg gcagagcaga tgtcgaaaag gccatttcgg
     5461 cagccagaaa tgctttcgac cacggccctt ggggcaagat gaccaaccgc caaaggagtc
     5521 ggctcctgtt tgaggccaga gacctcttgt cacggcggtc ggaggaattc gccgcgctgg
     5581 agacactaga ttgcgggaag atcttcaccg gggctttgca ctatgacgtg gctcaagcgc
     5641 tggacggttt cgaatactac gccggcaaag ctcgctgtct ggaaggaaac gtcatacccg
     5701 ttgacggagg cggcagatac ctcaactatg tgatgtggca gccctacggc gtggtcggcg
     5761 agatactgcc gtggaactgc ccgctgttga tgggagccgc gaagatagcg tccatactgg
     5821 cggcaggcaa cacagtcatc atcaagcctt catcctgggc ttccctgagc atgttgacaa
     5881 tggcggaggt acttcacgaa gccgggttcc cgccgggggt tgtcaatatc gtgaccggat
     5941 ccggaagcga ggtcggcact gcactggttg agagtccttt ggtggacatg gtgtccatga
     6001 ccgggggtac cgacacggga aggaaggtca tttccgattc cagcaagacc atcaaggacg
     6061 ttgccctgga gctgggcggc aagagcccga acatcgtctt tcccgatatc gactgggagt
     6121 ctgccgtcaa gtggactctg ctcggcttca ccctgggctc cgggcaggtg tgcgtctccg
     6181 gcactcgtct cattctgcat cgatccatat acgacagatt cctggctcag ctgaaagcgg
     6241 agtgtgagaa gctgattccc ggcgacggtt tcgactgcga aagagaagtg aacctgccgt
     6301 ccctcattca cccggatcat gcagcccggg tgtggagcta cattgaaaag ggcaaggccg
     6361 aaggcgcccg tctgattacc ggcggcgtcc catatgacga cccggcgctg gccaaaggca
     6421 atttcgtccc tcccacagtg tttgccgatg tcacccctga gatgaccatc ttccgagagg
     6481 agatcttcgg tccggttctt gccgtgacgc cttttgagac tgaacagcag gctatagacc
     6541 tagccaacga tacgcctttc ggcctggctg gagctgt
//
LOCUS       DAOTAC010000131         6555 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_9980_length_6555_cov_4.879692, whole genome shotgun sequence.
ACCESSION   DAOTAC010000131 DAOTAC010000000
VERSION     DAOTAC010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6555
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9980_length_6555_cov_4.879692"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            523..2265
                     /locus_tag="PLM74_06745"
     CDS             523..2265
                     /locus_tag="PLM74_06745"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HON42808.1"
                     /translation="MPARAGKVRMLVSCPASLVRRSRFSTNPRIVALILVCAFVLSAF
                     WSAIGAAATTSSETGKSQSPYLIIHLDAVSSETFFELYDSGCLPNIEAAFRNGKIVRH
                     AVSPFCPGTEMVYPRLTNGGRIPDAYPVAWEYLDRATGQHIGHVQTTVDLFSGIPRYA
                     LTQVVHALPGLEWLSALSLLNAPRLMRDYDVVQFFWFATDLRGHMFGKQSQQASVMQL
                     DRFIGNLMLQMSGESLNLVIYSDHGMSYFQHMVDPDKAMAEAAAGEAEFCYYPNLYLR
                     NPAQAERIASELASCDEIDFAFYRSGPGMVAGVHAGGKVAFSAGPAGIAYGFEGSDPF
                     GYCDLGYAGEPLSDQSWLSLTAGSRYPAVPVQVFRYMESPLSGDVVVVIDPPKGLLTP
                     GCPVGCHTGLTDSAVTVPVLLVGPDIDDMVIDDQFWLHTLYRDILGVDPHEPPSASRE
                     PHYLKASPHSFSLALSPARQLYVHANVDSCGWDVLGELSVARTFNTRVWVGAGIAARQ
                     WTEDESIRTSGSSLQAALSARAEVFLGPLVVDWQNTVTPAGRASRVSIRYEFKGGSLI
                     EWVHPSHLRVGIMW"
     gene            2601..2942
                     /locus_tag="PLM74_06750"
     CDS             2601..2942
                     /locus_tag="PLM74_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020542609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HON42809.1"
                     /translation="MRLHGPDEPVYTISVAARLLGVSPQTLRVMEREGLLSPARTDAN
                     IRLYSENDLLLLQRICFLVREEGVNLAGVKLILEMETGGGHTTVRFTEVIRRQQAVRR
                     QSDPDDNDDHS"
     gene            3002..4954
                     /gene="dnaK"
                     /locus_tag="PLM74_06755"
     CDS             3002..4954
                     /gene="dnaK"
                     /locus_tag="PLM74_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019490128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HON42810.1"
                     /translation="MGKVVGIDLGTTNSVIAVMEAGNPEVIVNAEGSRLTPSVVGFTK
                     SGERLVGQLARRQAVLNPENTVSSIKRFMGRRYDEVEAERGRAPYKVKSGDNYGVRMD
                     VPATGRDYSPEEISAMVLQKMKTDAEKFLGETITKAVITVPAYFNDSQRQATKDAGRI
                     AGLEVLRIINEPTAAALAYGLDKKSNETILVWDLGGGTFDVSILEVGEGVFEVKSTAG
                     DTHLGGDDYDQRIVEYIADQFKRDQGIDLRRDRQALQRLIEAAEKAKIELSSVFETNI
                     SLPFITADQSGPKHLEMKITRAKFEELTDDLTERCTVPFKQAINDAKLSVNQIDEVIL
                     VGGATRMPVIQELVKKLTGKEPNRGVNPDEVVAVGAAIQAGVLAGEVKDVVLLDVTPL
                     SLGIETLGGVSTKLIDRNTTIPTRKSQIFTTAEDGQTSVEIHVLQGERDFARDNRTLG
                     RFMLQGIPPAPRGIPQIEVSFDIDANGIVNVAAKDLGTGKEQRITITASTQLSKDEVD
                     KLVRDAERSSAEDKKRREEVELKNTADSMVYSTEKTLREMGDKIPADTKSKIEAELER
                     LKKAIADNDIGSIKRFMEDVRQASYKMSEELYKQAQAAGAAGAGGAGGAGQQPGAEGG
                     PSAGDAGRHSADDDVIDADFRPMDDK"
     gene            4989..5435
                     /locus_tag="PLM74_06760"
     CDS             4989..5435
                     /locus_tag="PLM74_06760"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HON42811.1"
                     /translation="MTLMNWDPWRDIADMRNTFNRLFGEATPGTAIAGHMVSGSWMMP
                     VDIAETADEFLVKAELPGMKPEEIEITVADNQVTLKGKRAESREEKSVNYIRSERRLG
                     SFYRSFALSAPVDDARVTATYKDGVLEVHLPKSEAVKPRQIRIQSE"
     gene            5571..6173
                     /locus_tag="PLM74_06765"
     CDS             5571..6173
                     /locus_tag="PLM74_06765"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HON42812.1"
                     /translation="MTSKSHDECAEQRSREQQACSRGHDADQTGQNDQPQPGQPRPAG
                     ESKPVGELETSLEEAKAKAAENWDLYLRARADLENYQRRVARDLDYNIRCGKRDMILR
                     ILAIVDDMERALAADADYESLHSGVDMIMRKILDVLAAEGVKQIEAVGQPFDPRFHDA
                     VVACEDPTVKTETVVDDLRRGYTYGDDCLRPTMARVAKPC"
     gene            6254..>6555
                     /locus_tag="PLM74_06770"
     CDS             6254..>6555
                     /locus_tag="PLM74_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HON42813.1"
                     /translation="MEFRDYYKTLGVSKTAGDKEIKSAYRRLARKYHPDVNPGDKSAE
                     ARFKEVAEAYEVLSDPDKRRRYDELGADWERYSKAGAGAGAGAGAGAGWPPGGFGRT"
BASE COUNT         1441 a         1926 c         2016 g         1172 t
ORIGIN      
        1 ggcagggaaa gatgactgct gtggctcagg gatatacccc cgggggtatc cagccggaga
       61 tacggtcaca ttctgacact agtttcggcg aaaaacgcaa ttgcgggtat ctccggaact
      121 gatggcaaaa atcgacgaca tgccagctcc agccgttttg tcccgcggcc atgatgataa
      181 gaacagctaa acagtcattc cagcgaagtg agtatgaccc cctcagccaa tagcccaccg
      241 taatgatctg ggaggatgcc attctcccgc ccactatccc gcctggcgat gtctactcgc
      301 ttgtccaaga tccgccatca tgattttcct gctgccaggc ttcccttggg ccattaccga
      361 gaccgcgcgt acccattgga atgagttggt ttcgtcattg gctttggtgg ccatagcaga
      421 ccgggcatgc tctgagctac acccagcgca gcggcgcgga ataggtccgg taagcccagg
      481 gcagattatg cgaggattgt caccaatatc cgatgtgtgc gcgtgcctgc gcgtgccgga
      541 aaggtgagga tgctcgtgtc atgcccggca agtttggtca gacgatcgcg gttttccact
      601 aatccccgca tagttgcctt gatactcgtg tgcgcattcg tgctgtctgc cttctggtcg
      661 gccatcggag ccgccgcgac cacttcatcc gagacgggca aaagtcagtc gccatacctc
      721 atcatacacc tggacgcagt gtcgtccgag acattcttcg aattgtacga ttcgggctgc
      781 ctgcccaaca tcgaggcggc tttcaggaac ggcaagattg tccgccacgc cgtctcccct
      841 ttctgcccgg ggactgaaat ggtctacccc aggctcacca atggcgggag aataccggac
      901 gcgtatccgg tcgcatggga gtacctggac cgggctaccg gccagcacat aggccacgtg
      961 cagactacgg tagatctctt ctccggaatc cccaggtatg cgctaaccca ggtagtacat
     1021 gccctacccg gactggaatg gctatctgcc ttgagcctcc tcaatgcccc gaggttgatg
     1081 cgcgactacg acgtcgtcca attcttctgg ttcgctaccg atctccgggg acacatgttc
     1141 ggaaagcaat cacagcaggc cagtgtgatg cagctcgaca ggttcatcgg aaacctcatg
     1201 ctccaaatgt ccggtgagtc gctcaacttg gtcatctact ctgaccacgg tatgtcgtat
     1261 ttccaacaca tggtcgatcc cgataaagcc atggccgagg cggcagcagg cgaagcggag
     1321 ttctgctact atccaaacct ctacctgcgc aatcccgcgc aggccgaacg gatcgcttct
     1381 gagcttgcca gctgtgacga gatcgacttc gccttctatc gatccggccc gggtatggta
     1441 gccggggtcc acgcaggcgg caaggtggcc ttctctgcgg gtccagccgg catcgcctat
     1501 ggattcgaag gtagcgatcc ctttggctac tgcgacctgg gctacgcggg cgaacctttg
     1561 tccgaccagt catggctaag cctcactgcg ggctcgcgtt acccagccgt tcccgttcag
     1621 gtgttccgct acatggaaag ccccctgtcc ggcgacgtgg tagtggtgat cgatcccccc
     1681 aaaggcctac tcacgccggg ctgccctgta ggctgccata ccgggctgac cgacagtgca
     1741 gtcacagtgc ccgtgctgct cgtcggcccc gatattgacg acatggtcat agatgaccag
     1801 ttctggctgc atactctcta ccgcgatatc cttggagtcg acccacacga acccccgtct
     1861 gcgagtcgcg aaccgcacta cctgaaagcc tctccccaca gcttcagcct ggccctctcc
     1921 cctgctcgtc agctctacgt gcacgcgaac gtcgatagct gcggctggga cgttctgggc
     1981 gaactcagcg ttgcacgcac gttcaacacc cgtgtatggg tcggcgccgg gatcgccgcc
     2041 cgtcaatgga cggaggacga aagcataagg acttcaggca gcagccttca ggccgcgctg
     2101 tccgcgcggg cggaagtgtt cctcggtcct ctggtagtcg actggcagaa caccgtcact
     2161 cccgcagggc gcgcgtcgcg ggtatcgatc cgttacgagt tcaaaggcgg cagcctgatc
     2221 gagtgggttc atccatccca cctgcgcgtg gggataatgt ggtgaccgct gaagacggat
     2281 tcacggtacg ctggtccttt ccccatcaga ttggactcgg gctgacctgg taggacagct
     2341 caagcctacg tgcgccagct tgcccgctcg ttccatggcc tgatcaaagg cgcaacggag
     2401 tccggcggtc aggccacaac gccgcaagtc ccagtcgcga atagtaccct gtccaaatcg
     2461 gcagattact gatagctcga cacacaagtc tgtcctgcaa atataacttt cttatattga
     2521 gtgtgttaag catattgaca tacgcgatac accccgccta taatgaccat aggaacccac
     2581 ttaacccgag gtgactagag ttgaggctac acggccccga tgaaccagtc tacacaataa
     2641 gcgtggccgc tcgtctgctc ggagtgagtc ctcagacgct cagagtgatg gaacgtgagg
     2701 gattgctgtc ccctgcgcgc actgacgcga acatcaggct ctactccgaa aacgacctgt
     2761 tgctgctgca gcgcatctgc tttctggttc gggaagaggg cgtcaacctt gcgggggtaa
     2821 agctcatcct agagatggag acgggcggag ggcataccac ggtcagattc accgaggtga
     2881 ttcgccggca gcaggcggtg cggcgccaga gtgatcctga cgacaatgac gaccactcct
     2941 gaatcgggtt tccagttcat ccatccagca caagctggtc aacggaagga gagagttctg
     3001 tatgggcaaa gttgttggca tcgacctcgg cactaccaac tcagttattg cagttatgga
     3061 ggccggcaac ccggaggtca tagtcaacgc agaaggttcc aggctgaccc cgtcagtcgt
     3121 gggtttcacc aagtcgggcg aacgcctggt ggggcagctc gcgcggcggc aagccgtgct
     3181 taacccggaa aacactgtat cctccatcaa aaggttcatg ggtcgccgct atgacgaggt
     3241 cgaagcggag cgcgggcgag cgccgtataa ggtcaaatcc ggcgacaact atggtgtcag
     3301 gatggacgtc ccggcgaccg ggcgcgatta ctcgcctgag gagatctcgg ccatggtcct
     3361 tcagaagatg aagacagatg ctgagaagtt cctgggcgaa actatcacga aggcagtaat
     3421 cacagtgcct gcatacttca acgactccca gaggcaggcc accaaggacg cagggcgcat
     3481 tgccggcctg gaagtgctcc gcatcatcaa cgagcccaca gccgcggccc tggcctacgg
     3541 gcttgacaaa aagagcaacg agacgatcct cgtgtgggac cttggcggag gaacgttcga
     3601 cgtgtccatc ctggaagtgg gcgaaggcgt attcgaggtc aagtccactg caggcgacac
     3661 gcatctgggc ggcgacgact acgaccagcg gatcgtggag tacattgccg accagttcaa
     3721 gcgagaccag ggcatcgacc tcaggcgcga ccgtcaggcg ctgcagcggc tgatcgaagc
     3781 ggccgagaag gccaagatcg agctgtcttc ggtgttcgag accaacatca gcctgccgtt
     3841 cattacggcg gaccagtccg ggccaaagca cctggagatg aagataaccc gggccaagtt
     3901 cgaagagctg acggatgacc tcaccgagag atgcaccgtc cccttcaagc aggccataaa
     3961 cgacgccaag ctctcagtga accagataga cgaggtgatc ctggtgggcg gcgccacgcg
     4021 catgcctgta atccaggagt tggtcaagaa gctcacgggc aaggagccga accgcggcgt
     4081 gaaccctgac gaggtcgtcg cagtgggcgc agcgatccag gcgggcgttc tggcaggcga
     4141 agtcaaggac gtagtgcttc tcgacgtgac gccgctgtcg ctcggcattg agaccctggg
     4201 cggcgtgtcg accaagctga tcgacaggaa cacgaccatc ccgacgcgca agagccagat
     4261 cttcaccaca gcggaagacg gccagacgtc cgtggagata cacgtgcttc agggcgagcg
     4321 cgactttgcg cgcgataata ggacgctcgg acggttcatg ctgcagggca taccgccagc
     4381 accacgcgga atcccgcaga tcgaggtatc attcgacatc gacgccaatg gcatagtcaa
     4441 tgtcgcggcc aaggacctcg ggacgggcaa ggaacagagg atcaccatca ctgcctccac
     4501 gcagctgtcg aaggatgaag tcgacaagct cgtccgcgac gctgagcgat catcggctga
     4561 ggataagaag cgccgcgagg aggtcgaact gaaaaacact gccgactcca tggtgtactc
     4621 cacggagaag acccttcgcg agatgggcga caagataccg gcagatacga aatccaagat
     4681 cgaagcggag cttgagcgtc tgaagaaggc catcgccgac aacgacatcg gttctatcaa
     4741 gcgcttcatg gaagacgtca ggcaggcatc gtacaagatg tcggaggagc tgtataagca
     4801 ggctcaggcc gcaggcgcag ccggagctgg cggggctgga ggagctgggc aacagccggg
     4861 cgctgaaggc gggccaagcg ccggcgatgc cggaaggcac agcgccgacg acgatgtgat
     4921 cgatgccgac ttcagaccga tggacgacaa atagacaggc agaagagacg caaaggaggt
     4981 gtgccgagat gacactgatg aattgggatc cgtggcgtga tatcgccgac atgagaaaca
     5041 cgttcaacag gctgttcggt gaggctacac ccgggactgc aatagcaggt cacatggtat
     5101 cgggcagctg gatgatgccg gtcgatattg ccgagactgc tgacgagttc ctggtcaagg
     5161 ctgagttgcc gggaatgaag cctgaggaga tcgagatcac tgtggccgac aaccaggtga
     5221 ccctgaaggg gaagcgcgct gagtcacgcg aggagaagag cgtaaactac atacgctctg
     5281 agaggcgact gggctccttc taccggagct tcgcgctgtc ggcgccggtg gacgacgcgc
     5341 gcgtgaccgc cacctacaag gacggcgtcc ttgaggttca cctgcccaag tcggaggcgg
     5401 ttaagcctag gcagatccgc atccaatcgg agtaacgcgg gcattgggat gacatgcagc
     5461 gactgtcggt cggggggggg caatcacctc cccgaccacc ccggcggggg cgccccgcag
     5521 gggaattcgc agcacgacat atacaacatg atcggtggtg cagctaatct atgacatcaa
     5581 aatcgcatga tgagtgcgcc gaacaacgga gtcgcgagca gcaggcgtgc agccggggcc
     5641 acgatgccga tcagaccggc cagaacgatc agccccagcc cggccagcca aggccagcgg
     5701 gcgagtccaa gccagtcggc gagctcgaga ccagccttga ggaggctaag gcgaaggccg
     5761 ccgagaactg ggacctttat ctgcgtgcac gcgcagacct ggagaactac cagcgccgcg
     5821 tggcgcgtga cctggactac aacatcaggt gcggcaagcg cgatatgatc ctgcgcatcc
     5881 tggcaatcgt cgatgacatg gagcgagcgc tggcggccga tgcggactac gaatcgctcc
     5941 acagtggcgt agacatgatc atgcgcaaga tcctcgatgt gctggctgcc gaaggcgtca
     6001 agcagatcga ggccgtgggt cagccattcg acccgcggtt ccacgacgcc gtcgtagcct
     6061 gtgaagatcc caccgtaaag acggaaaccg tcgtggacga cctgaggcgc gggtacactt
     6121 acggggacga ttgcttgagg cccaccatgg cccgggtggc gaagccgtgc tgaggcgccc
     6181 actctgtgcc ggcgccgact ccggcttccg ccgtcgcggg ataatctgat ggtgtgcgcc
     6241 ctggagatag acaatggagt tcagagacta ctacaagacg cttggcgtgt ccaagactgc
     6301 cggcgataaa gagataaagt ccgcgtaccg ccgcctggcg cgcaagtatc acccggacgt
     6361 caaccccggc gacaagtccg ccgaggcgcg cttcaaagaa gtcgccgagg cgtatgaggt
     6421 gttgtctgat cccgacaagc gccgaagata cgacgagctg ggggcggatt gggagcggta
     6481 ctccaaggcc ggcgcgggag ccggtgcagg tgcaggtgca ggtgccgggt ggcctccggg
     6541 cgggttcggg cgcac
//
LOCUS       DAOTAC010000132         6492 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_10097_length_6492_cov_4.494019, whole genome shotgun sequence.
ACCESSION   DAOTAC010000132 DAOTAC010000000
VERSION     DAOTAC010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6492
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10097_length_6492_cov_4.494019"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="PLM74_06775"
     CDS             complement(<1..570)
                     /locus_tag="PLM74_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42814.1"
                     /translation="MDSRFRRTSVMAYLVAIAMLLISAEALAVPACPVPITLAQPDGT
                     SFRATLWGDEWLNGAETMDGYAVVQDQVTGYWEYARRSVKGDLAPSGLRPGPHDPASE
                     GIERGLRPSLGMGARGLDHGDVSLPAAAPSVETVNVPVILITFSDRTPKYSMANFQDL
                     LFGDHPSIATGPGSMKDYYEEVSYGAFSVS"
     gene            complement(570..1433)
                     /locus_tag="PLM74_06780"
     CDS             complement(570..1433)
                     /locus_tag="PLM74_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42815.1"
                     /translation="MAEYHPRSAHRIWTLAIIILVSSTLVGCGSGTSVPTYSISGRVT
                     RASDHTGLDGIILQFSGGFGTTTTSNGGYWNKTGVKGIVTVTPVGQGWTFAPPNREVS
                     GAASDVDFEAMPIGPYSVSGRVADQGGAGIADVLLFLGGGFGTARTASDGCWSATGLS
                     GDVTVTPAKAGLVFTPETVTVSGWSSSIHFTGSPATNTYSVSGRVTNSSDGSGLYGVL
                     FTFSGGFGRAQGDASGYWSKSGLYGTVTVTPSRPGWEFQPQSCTVTSASNAADFVGWR
                     PGSSAAKGGDK"
     gene            1678..2859
                     /gene="pgsB"
                     /locus_tag="PLM74_06785"
     CDS             1678..2859
                     /gene="pgsB"
                     /locus_tag="PLM74_06785"
                     /inference="COORDINATES: protein motif:HMM:TIGR04012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0070501 - poly-gamma-glutamate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate synthase PgsB"
                     /protein_id="HON42816.1"
                     /translation="MYMFAALCVCLLGAGVVENLLHQRCLKRIPVRVHVNGTRGKSTT
                     TRLIAAGLRAGGLRVIAKTTGTAARLIMEDGSELPVARRSGRANISEQMRIVRLAARQ
                     QADAVVLECMALEPENQWVIERRMVRSTIGVITNARPDHLDVMGPTAADVAQVLALSV
                     PYRGHLVTAEREYLPMFERAAEKAGTELHTVDGSDVPDETNAEFEYVSFKDNVGCALR
                     VCEIAGVAREVALAGMAAAAGDPGAMTLRKITLGGADYVFGNAFAANDQDSTLAAWRL
                     FVDQAQAGSQLPTAVIMNNRSDRLPRIAEMAALIAQEIRPSVVFVVGESGRLAARALR
                     RAGVPMERVVDLTRIREARLAVDSMAAECKEGAWLFGIGNTRGAGQCIADYFLENGVP
                     F"
     gene            2856..3293
                     /gene="pgsC"
                     /locus_tag="PLM74_06790"
     CDS             2856..3293
                     /gene="pgsC"
                     /locus_tag="PLM74_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005486914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0070501 - poly-gamma-glutamate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein PgsC"
                     /protein_id="HON42817.1"
                     /translation="MTVALSVGIGLIVSFVYTELTGFLGGGLVVPGYLALYMNQPARI
                     AATFLAALAAYAVVWALSNVVILYGRRRFVACILAGFWIGWAMARVGIKIGWGGHELR
                     TIGYIIPGLIANDIARQGVLRTVSSVVLLSVTVRLILILAGLI"
     gene            3348..>3558
                     /locus_tag="PLM74_06795"
     CDS             3348..>3558
                     /locus_tag="PLM74_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42818.1"
                     /translation="MRTRRRRIPIAVADSIANATAGLVPFGRGFSRSHVRVLGIVLAA
                     VLAVTGLSLAEGPSGAPIALRGGPGA"
     assembly_gap    3559..3568
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3569..4650
                     /gene="pgsW"
                     /locus_tag="PLM74_06800"
     CDS             <3569..4650
                     /gene="pgsW"
                     /locus_tag="PLM74_06800"
                     /inference="COORDINATES: protein motif:HMM:TIGR04332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="poly-gamma-glutamate system protein"
                     /protein_id="HON42819.1"
                     /translation="DRQIRAAEIMRDCTLAIAQERTALGIHIDPELDPNGTGLIGAEF
                     TDITTTLGVLEAKRTSTNPAFAALMVRYFLEAGLEPGDVIAVGASGSFPALILATLAA
                     CRALDLQPAVIYSIGASMYGANIPGFTFADMLIMLNRRGLLRCRLAAVSLGGEGDAGA
                     NGILGQGSECFEQAATRSGAPVLKEGTISDSIRRRMEVIAETAETLGRPVACFVNVGG
                     ATANYGNTSASLDFPNGLVMHPTVMSAHPERGLIFEYAAMGVPVINLLDVRGLAIRNG
                     LPVDPIPLPSPGEGGVYFTTAHSRPAAALALLASAGAVLGAAGFLRPSHHVAKRHGIR
                     VPGAHAQQGDGFVDNVARRDRQGIT"
     gene            complement(4967..5365)
                     /locus_tag="PLM74_06805"
     CDS             complement(4967..5365)
                     /locus_tag="PLM74_06805"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HON42820.1"
                     /translation="MRDYHWVDYYRTIGVGRHATADQIRAAYRARARMCHPDVCRDPN
                     AAHTFTQLVEAYGVLSDAEKRKDYDAFLDRDRALPGTQVRFHSRLRPIIDIVVRWLPG
                     DPLPFLMQALQVIGLSLAAVILLLAALYVL"
     gene            complement(5456..6064)
                     /locus_tag="PLM74_06810"
     CDS             complement(5456..6064)
                     /locus_tag="PLM74_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HON42821.1"
                     /translation="MPNVLAFLSYILAMTITPGPNNMMCLVNGSRFGFRKTLHFIAGI
                     FAGVVVVMTACSYFNLSLSRFIPSFRPFAEALGGLYMLYLALRVLKTDVNAATANARS
                     ATFVTGIGMQFVNAKMLLYGLTVTANFITPYYQSLVILILFSLFLACCSALAASCWAL
                     FGTTIQRLFGQYRKPLNIGMSLLLLYAALSISGILPRLRLLL"
     gene            complement(6240..>6492)
                     /locus_tag="PLM74_06815"
     CDS             complement(6240..>6492)
                     /locus_tag="PLM74_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42822.1"
                     /translation="RKAGVYSLVVTRYSPADNHQLELVGLVDLTGKAGLVIPRYTYML
                     AQAVELSIGAITGFGDNTSPLGAAVPEARGVIAGIKASF"
BASE COUNT         1346 a         1929 c         1922 g         1285 t
ORIGIN      
        1 cgataccgag aaggccccgt atgacacttc ttcatagtag tccttcatgc tgccggggcc
       61 ggtagcgatg ctcgggtgat cgccaaacaa gaggtcctgg aagttggcca ttgagtactt
      121 cggagttcgg tcgctgaatg tgatgagaat cacagggacg ttgacagtct caactgatgg
      181 agctgcggca gggagcgaca cgtcgccatg atccagccca cgcgccccca tcccaaggga
      241 cggacgcagc ccacgttcta tgccctcgct agccggatca tgcggccctg gccgaagccc
      301 ggatggcgcc agatcgccct tcaccgaccg gcgcgcgtac tcccagtacc cagtgacctg
      361 gtcctgaact acggcatatc catccatggt ctctgcacca ttgagccact catctcccca
      421 cagcgtagcc ctgaaggacg taccatctgg ttgtgcaagt gtgatcggga ccgggcacgc
      481 gggtacagcc agtgcctctg cggatatcaa gagcatggca attgccacga gatacgccat
      541 aaccgaagtg cgtctgaatc gtgaatccat cacttgtcgc ctcctttggc agcgctgctg
      601 ccggggcgcc acccaacaaa gtccgccgca ttgcttgcac tggtcacagt gcatgactgc
      661 ggctggaatt cccaccctgg ccgcgacggc gtgactgtaa ccgtgccata cagcccggac
      721 ttggaccagt agcctgaggc atcgccctgt gctcggccga atcctccgct gaatgtaaac
      781 aagactccat acaggccact gccgtcacta ctgttggtga cccgccccga cactgagtat
      841 gtgttcgtag cggggctccc cgtgaagtgg atgctgcttg accaaccaga cactgtgaca
      901 gtttcgggcg tgaacacaag ccccgccttt gccggtgtca cagtgacatc accggacagg
      961 cctgttgcgc tccaacaacc gtcagacgcg gttcgggccg tgccaaagcc acctccaagg
     1021 aacagaagaa catcggcgat ccccgctccg ccctgatcag ccacacgtcc agatactgag
     1081 tatggcccaa tcggcatggc ctcgaagtcc acatcgctag cggcaccgga aacttccctg
     1141 ttcggcggag cgaaagtcca tccctgcccc acgggcgtaa cagtcactat tcccttcaca
     1201 ccggtcttgt tccaataccc cccgttggag gttgtggtag taccgaatcc tccactgaat
     1261 tggagtatga ttccgtcgag acccgtatgg tcgctcgccc gggtaacacg accggagata
     1321 ctataggttg gaacgctcgt acccgaaccg cagcccacga gtgtcgagga taccaagatg
     1381 attatggcta gcgtccagat tctgtgggca cttcttgggt gatactcagc caatgtgccc
     1441 ctcccttacg tgtcagtcga cgcggtgccg ctcatggtca atgctccctg ctgtgccatt
     1501 gaactacgcc tcgttacttc tacaagttac ctctttcccc ttcgcgatct gacattgata
     1561 ccatatccgg tattcgttca gcgcgttgcc gtgcactccc ctgtaccggc gagccaggtg
     1621 gaaggagagg ccagcgtaaa cggcgaatac aagtcctgcg ggaggcgatg ggcgggtatg
     1681 tacatgtttg cggctctctg cgtctgtctt ctaggcgcag gtgttgttga gaacctcctt
     1741 caccagcgat gcctcaagcg aataccagtg cgtgtgcatg tcaacggcac acggggcaag
     1801 tccactacca ccaggttgat cgctgcaggg ctgagggccg ggggcctccg ggtgattgcc
     1861 aaaaccacag gcacagcagc caggctgatt atggaggacg ggtctgaact ccctgtcgcc
     1921 aggcgcagcg gtcgcgctaa catatccgag cagatgcgca tcgtccggct agcagcccga
     1981 caacaagcgg acgctgtggt gctggaatgc atggcgctgg aacctgagaa ccagtgggtg
     2041 atcgagcgcc gcatggtccg gtcgacgata ggcgtgatca ccaacgcgcg gcctgatcac
     2101 ctagacgtga tggggccgac cgctgccgac gtggcccaag tcctcgccct aagcgttcca
     2161 tatcgcggcc atctggtcac agctgagcga gagtatctcc ccatgttcga acgggcggcc
     2221 gagaaagccg gcaccgagtt gcacacagtg gacggctcgg acgtgccgga cgagactaac
     2281 gccgagttcg agtatgtgag tttcaaagac aacgtggggt gtgcgttgcg cgtatgtgaa
     2341 attgcgggtg tggcacgcga agtagcgctg gccggaatgg cagcagccgc aggcgaccca
     2401 ggtgccatga ccttgcgcaa aatcactctt ggcggcgcgg attacgtgtt tgggaacgct
     2461 ttcgcggcca acgaccagga ttccacactc gccgcgtgga ggttgtttgt cgatcaggca
     2521 caagcaggat cgcagctgcc gacagcagtg ataatgaata acagatctga ccgtctgcct
     2581 cgtatcgcag aaatggctgc gctgatagcg caggagatta ggccatccgt ggtgtttgtg
     2641 gtgggcgaat caggaagact ggctgcgcgt gccctgcggc gtgcgggagt gcccatggag
     2701 agagtagtgg accttaccag gatccgcgag gcccggttgg cagttgacag tatggcggcc
     2761 gaatgcaagg aaggcgcgtg gctgttcggg atcggaaaca ccagaggcgc cgggcaatgc
     2821 atcgcagact acttccttga gaatggggtg ccgttttgac tgttgcacta tcagtcggaa
     2881 tagggttgat cgtcagcttc gtctacacag agcttaccgg cttcctgggc ggcggtttgg
     2941 tcgttccggg ttacctagcg ctgtacatga accagcctgc aagaatcgct gccacttttc
     3001 tcgcagcgtt ggcggcctat gcggttgtgt gggcgctatc gaacgtggta attctctacg
     3061 ggcgccgccg attcgttgcc tgcattcttg cagggttctg gataggctgg gccatggccc
     3121 gcgtaggcat caagatcggc tggggtgggc atgaactgcg caccatcggc tacatcatcc
     3181 cgggactgat cgccaatgac atcgccaggc agggcgtatt gcgcacagtg tcctccgttg
     3241 ttctgctttc agtgacagtg cgccttattc tgatcttggc cggcctgatc tgatcggaga
     3301 cggcagcatc tcgaagacgc aagacacaag gaggtgaatg gaaccaaatg cgcacgagaa
     3361 gacgacggat acccattgcc gttgccgact ccatcgccaa cgcgactgcc ggtctcgtac
     3421 cattcggacg cgggttcagc cgaagccacg tccgcgtcct cggaatagtg ctggcggcag
     3481 tgctggccgt taccggcctc tcgctcgcgg aagggcccag cggggcaccg atagccctgc
     3541 gcggcgggcc tggtgcagnn nnnnnnnnac gaccgccaaa tccgagcggc cgagatcatg
     3601 cgcgattgca cactcgccat agcgcaagag cgaacggcgc tggggattca catagatcct
     3661 gaactcgatc ccaacggcac cggcctgatt ggcgcagaat tcaccgacat cactactacc
     3721 ctaggggttc tcgaagccaa gcgtacatca acaaaccccg cgttcgctgc attgatggtc
     3781 cggtatttcc tcgaagcagg gcttgagccg ggcgatgtga ttgcagtagg agcaagcggt
     3841 tccttccccg cgctcatact agcgactctt gctgcatgca gagcgcttga cctccagccc
     3901 gccgtgatat actccatagg cgcgtccatg tacggcgcga atatcccggg cttcactttc
     3961 gccgacatgc tgatcatgct caacaggcgc ggtctgctgc gttgccggtt ggcggcggtg
     4021 tcgcttgggg gtgaaggcga tgccggagcg aatggaatcc tcggccaagg ctcggagtgc
     4081 ttcgagcagg cagcaacccg atcaggggcg cctgtactaa aggaaggcac aatctccgac
     4141 agcatcagac gccgaatgga ggttattgcc gagacagccg agacgctggg gaggccagta
     4201 gcatgctttg tgaacgtggg gggtgcaacc gccaactacg ggaacacttc tgcttcgctt
     4261 gacttcccca acgggctcgt catgcatccc acagtgatgt cggctcatcc tgaacgtggg
     4321 ctgatcttcg agtacgccgc catgggagtg cctgtgatca acctgctgga cgtgcgcggc
     4381 ctggcaatca gaaacggcct gcctgtggac cccatacctc ttccgtctcc cggcgaaggc
     4441 ggggtgtact tcactacagc gcattcgcgg cccgccgcag ctctcgccct gctggcgagc
     4501 gcgggcgcag ttttgggagc agctggcttc ctgcgaccga gtcatcatgt cgcgaaacgc
     4561 cacggcatcc gcgtgcccgg cgcgcatgcg cagcaaggtg acggcttcgt cgacaacgta
     4621 gctcgacgtg accgccaggg cattacgtga cagcagttcc gcaaatgctt ccctggtgtg
     4681 atcgtgattg gtatccttgg gggccaggaa cgctgtccat gcgcttgtgt caacgaacag
     4741 cttcacggct ggcaccccgc acgtccctcg cgaccgttgc gatcgttata accatcatta
     4801 tcgatccaat cctcgggcgc ttcgcaccgc gaatagaggt cgcggtcgtg ttcttcgctg
     4861 ccatctaccg cttcgcaggc aatactgaga gtcagtagtt cggccaacgg gttcgacagc
     4921 gcacatgaca tgattgcgcg aagacgctgc cttgccgctg cagggtctac aggacgtata
     4981 gggcggccag cagtagaatg actgcagcca gagacaggcc aattacctgt agcgcctgca
     5041 ttaggaaggg caaaggatcc ccgggtaacc agcggacgac gatgtcgatg atgggacgaa
     5101 gacgggaatg gaatcgaact tgtgtcccag gaagcgcccg atcgcgatcc aggaaggcat
     5161 cgtagtcttt tcgcttctcg gcatcgctca gcacgccata ggcctccaca agctgggtga
     5221 atgtgtgggc agcgttcggg tcgcggcaca catcggggtg acacatgcgg gcccttgcgc
     5281 gatacgcggc gcgtatttga tccgcggtag cgtgacggcc tacgccgatg gttctatagt
     5341 agtcgaccca atggtagtcc ctcatctccg cgcccacccc cgagtggcgc tttcattcga
     5401 tgccacggta gccgactcct gcgaacactg ataatcacga taaatggggg cgggctcaga
     5461 gcaacagcct gaggcgcggc agtatccccg atatagatag tgcggcgtac agcaatagca
     5521 atgacatgcc aatgttgaga ggtttcctgt actgaccgaa caagcgctga atagtagtac
     5581 cgaacaatgc ccagcaagac gccgctaagg ccgagcagca cgcgaggaag agcgagaaca
     5641 gaatcaagat aacgagggac tggtagtatg gagtgatgaa gttcgcagtc accgtcaggc
     5701 cgtacaggag catcttcgcg ttcacaaact gcatgccgat gccggtgaca aacgttgcac
     5761 tacgtgcgtt cgcggttgcc gcattcacat ccgtcttgag cacgcgcaaa gccagataca
     5821 gcatgtacag cccgccaagg gcctcggcga acggacgaaa gctggggatg aaccgcgaca
     5881 gcgacaggtt gaagtagctg caggcagtca tcaccactac cacacctgcg aagatgcccg
     5941 caataaagtg aagcgtcttg cggaagccga acctgctccc attgaccaga cacatcatgt
     6001 tgtttggacc cggcgtgatc gtcattgcca gtatatagga caagaacgca agtacgttgg
     6061 gcattccgga atctctcccc actcgccatc taccatgcct acatgctacg tggaattcgc
     6121 tgtgagttct acgttttcct tgtgtattcc atgcgtatcc ctcgctgcct acagtacata
     6181 gcttcgcccc cacctcatcg aatgggggcg aagatgctct atgccttgtg ccagaggtac
     6241 tagaagctgg ctttgatccc ggcaatcacg ccgcgggctt ccggcacagc agccccgagc
     6301 gggctcgtgt tgtctccgaa tcctgtgatc gccccaatcg aaagttccac tgcctgcgcc
     6361 agcatatacg tgtagcgcgg gatcacgagc cccgccttgc ctgtgagatc cacaaggcca
     6421 accagctcca gctgatggtt atcggccggg gaatagcgcg tcactaccag ggaatagacg
     6481 cccgctttgc gc
//
LOCUS       DAOTAC010000133         6461 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_10154_length_6461_cov_4.472057, whole genome shotgun sequence.
ACCESSION   DAOTAC010000133 DAOTAC010000000
VERSION     DAOTAC010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6461
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10154_length_6461_cov_4.472057"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1066
                     /gene="hutI"
                     /locus_tag="PLM74_06820"
     CDS             <1..1066
                     /gene="hutI"
                     /locus_tag="PLM74_06820"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HON42823.1"
                     /translation="GFVDAHTHLVFAGERYREHVMRPGGAKYLDILAAGGGILSTLTS
                     TRSASLAELVAGAMKRLDTMLLHGTTTVEAKSGYGLDIDTELRQLEAARLVSEAHPVD
                     VVSTFMGAHAVPPEFKGDPEGYLDFVLSEALPAVAEQGIAKFCDVFCEEGVFSVEQSR
                     EVLRAGMEHGLRPKIHADEIFPIGGAQLAAELRAVSADHLAAASPEGIRSLARAGVVA
                     VLLPATMFTLMSEAYADARGMIDAGVAVALATDFNPGTSPTMSMPFVITLACLQMKMT
                     PAEAVCAATRNAAWAIGRGDRAGTIQVGRDADFIVCDVPAVDALPFSVGVNPVRDVVK
                     RGVHVVRNAGGGGVLCRSRI"
     gene            1048..1680
                     /locus_tag="PLM74_06825"
     CDS             1048..1680
                     /locus_tag="PLM74_06825"
                     /inference="COORDINATES: protein motif:HMM:NF016825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HON42824.1"
                     /translation="MSFADMTVRGFLDELASRSPAPGGGSASAVAGAMGVGLAAMVAG
                     LTDTSVMAAEDAGRVNEIMQRSKGLIEALLGCADDDTAAFNEVMKAFKLPKATDEEKR
                     ARREAIQLSLKGAVNVPRRTLQLAAEGMQMAANMAELGNDNAASDAGVGALLLDTAMG
                     GAIFNMQINLASIKDQDFVAQMKSEIDHYAQLRDELRQRAVHSVGRRIGS"
     gene            1731..2981
                     /gene="folP"
                     /locus_tag="PLM74_06830"
     CDS             1731..2981
                     /gene="folP"
                     /locus_tag="PLM74_06830"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HON42825.1"
                     /translation="MEDWDGGSVGARVVALDSEGSVRAELAAMGVSRAGVDIMAPKSV
                     FRAVRLHKVPLRAALVIKQEMLAKGGEAALPYAAAGLSEERCDVLLSGTLRHFSRLTE
                     TLRRQPFGLPAIAREIDAALAAFDGTPEPMHIGGRTFRFGERTYVMGILNVTPDSFSD
                     GGQHLSCDDALRHAEAMLESGADIIDIGGESTRPGAAPVSEEEECARVLPVVRLLATR
                     SNAIISIDTYRANVARKCLDLGAHIINDITAFRGEGDMAAVASQYGAPVILMHMLGHP
                     TDMQVDPAYDEVVHEVCEYLRQRVAYACERGVSVCNTIVDPGIGFGKTPQHNLEIMRR
                     LRELKSLGRPILVGTSRKSTIGKVLGRPVHDRVWGTAATVALAIASGADMVRVHDVAE
                     MATVARMTDAVTRGWRGEEDGNAG"
     gene            2971..3396
                     /gene="folB"
                     /locus_tag="PLM74_06835"
     CDS             2971..3396
                     /gene="folB"
                     /locus_tag="PLM74_06835"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HON42826.1"
                     /translation="MQADRITLKNLVFYAYHGVFAAEKELGQRFEVDIDMYGDFQTAA
                     RADDIDMTVNYGDVYALVKEIVEEREFDLIEALAGTIGDEVLAGYPVSRVTVRVRKPN
                     APLGGPADYVECEISRGAAGGGQPGPRCEDDVCEGEAEL"
     gene            3374..4216
                     /locus_tag="PLM74_06840"
     CDS             3374..4216
                     /locus_tag="PLM74_06840"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HON42827.1"
                     /translation="MKVKPSFDILQVEETDSTNRMARVLAESGACDGTVVVAGRQTAG
                     RGRRGRSWLSSGDFGLWFSVVLRPEFEPNLAGLLGIGAAVAVARAVDKLTGVSADLKW
                     PNDVLIGGRKVCGVLAEASTAAGRVEWAVVGVGINLFHPEGGFPPEISPVATSLSEHM
                     GPEAAIDRERLLGAVLDELADRLALLEREGARGACELVDEAERLMRSMMGRRITVKAA
                     SGQFEAVAVRLDHEGGLVVANDCGESIRVTSADVSIGYGPAESAESAQSAEPAEPAEE
                     APAR"
     gene            4213..4815
                     /locus_tag="PLM74_06845"
     CDS             4213..4815
                     /locus_tag="PLM74_06845"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HON42828.1"
                     /translation="MNKAIALAVAALMVGMSCRAPAVAAAPSVPAARTAAAPGRLTLE
                     PGWTDIARIITNLGGAGASVALSAALLAYDPPTGKMMLNATACTAGVVEALKIGLGRA
                     RPYVPGDGGRFTGPNLSPDYHSMPSGHTANAFAMATVLSHKHPDWSPVLYGLALAVGL
                     SRIELGVHWPSDVVIGAGIGLAVGIQVTQGRLRLFAFDND"
     gene            complement(4981..5475)
                     /locus_tag="PLM74_06850"
     CDS             complement(4981..5475)
                     /locus_tag="PLM74_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42829.1"
                     /translation="MRRRAPARDVLRYDPLLFADGGHSADAGANPDAMSNADADAYTC
                     AMPDVSARDDVAAGTAGTMPRADNAHACAVPYSDAVPWPRSHAAADSHACAMPGAMAD
                     ADAMSNADAMSNTDAMPNAYAVPDAHAGDAGADPVSTVLPVPTASVPHNAGAGPHAAS
                     SRIP"
     gene            5739..>6461
                     /locus_tag="PLM74_06855"
     CDS             5739..>6461
                     /locus_tag="PLM74_06855"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HON42830.1"
                     /translation="MADERRSIDLSVTGMSCAACAARVERSLKHEPGIHQASVNLASE
                     TARVLYDPGVVDPDRIIQVVENAGYSARELVRDRREDDARKAEDRRRAGMNWGLFWVG
                     AVLSLPMVIGMLAELAGVHSLMFLMDPVLGFVLATPVVLVPGSRFYISAYKTVTHGGA
                     NMDVLVALGTGAAYVLSVVNTFFARGPVYYEAAAVVITLVLLGKNLEHGAKGRASEAI
                     RKLVSLTPRTATIMRGEIEETVA"
BASE COUNT         1195 a         1755 c         2259 g         1252 t
ORIGIN      
        1 gggatttgtc gatgcccata ctcacctggt gtttgccggg gaacgctacc gcgaacatgt
       61 gatgcgcccc ggtggagcca aatacttaga tatcctggcc gcaggtggcg ggatcctgtc
      121 gacgctcacg tcaacccgct ccgcgtcttt ggccgaactg gtcgcgggcg caatgaagcg
      181 gctggacacc atgcttcttc acgggaccac tactgttgag gccaagagcg gttacggcct
      241 ggatatcgat actgagctgc ggcagctgga agccgcgagg ctcgtgtcgg aggcacaccc
      301 ggtcgacgta gtgagcacct tcatgggagc tcacgcagtg ccgcccgagt tcaagggaga
      361 tcccgaggga tacctggatt tcgtgttgtc ggaggcgctg ccggccgttg cggaacaggg
      421 aatcgccaag ttctgcgacg tgttttgcga ggaaggcgtc ttctccgtcg agcagtctcg
      481 ggaggtgctt cgtgcaggga tggaacatgg cctccgtccc aagattcatg cagatgagat
      541 ctttcctatt ggcggcgccc agctcgccgc tgagctaaga gcggtatccg ccgatcacct
      601 ggctgcggca tcgcctgagg gcattcgaag cctggcccgt gcaggcgtgg tggcggtgtt
      661 gcttccggcg acgatgttta ccctcatgtc cgaggcgtac gccgacgcgc ggggcatgat
      721 cgatgccggt gtcgccgttg ctctggcgac cgacttcaac ccgggaacat cccccacaat
      781 gtccatgcct tttgtgatca ctctggcctg tcttcagatg aagatgactc cagctgaggc
      841 tgtatgcgcc gccactcgca acgcggcctg ggccatcggg cgtggcgacc gcgcgggaac
      901 catccaggtt gggcgcgatg ccgacttcat tgtgtgcgat gtgccggctg tggatgccct
      961 tccgttctcg gtgggtgtaa acccggtcag ggatgtagtc aagcgcgggg tgcacgtggt
     1021 aaggaacgca ggagggggag gcgtattatg tcgttcgcgg atatgaccgt tcgtggtttt
     1081 ctggatgaac tcgcgtcgcg aagcccggct cccggaggtg gcagcgcatc ggcggttgcg
     1141 ggcgcaatgg gcgtaggcct ggccgctatg gtcgccggcc ttacggatac gtccgtgatg
     1201 gcggccgagg acgcaggccg cgtcaacgag attatgcaga ggtcgaaggg gctcattgag
     1261 gccttgcttg ggtgcgccga tgatgatact gccgcgttca acgaggtaat gaaggccttc
     1321 aagctgccca aagctactga tgaggagaag cgcgcgaggc gggaagccat acagctttcg
     1381 ctgaagggcg cggtgaacgt tcctcgtcga acgctgcaac tggccgcgga gggaatgcag
     1441 atggcggcga acatggcgga gttgggcaat gacaatgcgg ccagcgatgc gggcgttggc
     1501 gctctgcttc tggacacggc catgggcggg gcgattttca acatgcagat caacctggca
     1561 tccataaagg accaggactt cgtggcccag atgaaatcgg agattgatca ttacgcccag
     1621 ctgcgggatg agctcaggca gcgggctgtg cattctgtgg gccggagaat cgggtcctga
     1681 ctcgctcact ggcgtttccg ggaacgatac tatcagaggg gatcatacgt atggaagact
     1741 gggacggggg cagcgtcggg gcgagggtcg tggcgctcga ttcggaaggt tcagtgcggg
     1801 cggagcttgc tgccatggga gtgagtaggg ccggggtcga tatcatggct cccaagagcg
     1861 tgtttcgtgc cgtccggctg cataaagtgc cgctcagggc ggctctcgtg ataaagcagg
     1921 agatgctggc caagggtggg gaggcggctc tgccttatgc tgccgcgggg ctgagcgagg
     1981 agcgctgcga cgtgctactt tcgggcacgc taaggcattt ctcgcggctg accgaaaccc
     2041 tgaggcgcca gcctttcggg ctgccggcga tcgcccgcga gatcgatgcg gctttagccg
     2101 cgttcgacgg cacacctgaa ccgatgcaca tcggcgggag gacattccgg ttcggggagc
     2161 gaacgtacgt gatgggcatc ctaaacgtaa ctccagactc cttttccgac ggcgggcagc
     2221 atttgtcgtg cgacgacgcg ctgaggcacg ctgaggcgat gctggaatcc ggcgccgaca
     2281 tcatcgacat agggggcgaa tccacgaggc cgggcgccgc cccagtctcc gaagaagagg
     2341 aatgcgcccg cgtccttccg gtagtccgtc tccttgcgac ccgttccaat gctatcatat
     2401 cgatagacac atacagagcg aacgttgcgc gaaaatgcct ggacctcggc gcgcacatta
     2461 tcaacgacat aactgccttc cgcggcgaag gcgacatggc ggcggtagca tcgcagtatg
     2521 gtgccccggt gatactgatg cacatgttgg ggcaccccac tgatatgcag gtagacccag
     2581 cgtacgatga ggtggttcac gaggtctgcg agtatctcag gcagcgcgtg gcatatgcgt
     2641 gcgagcgcgg cgtgtcggtg tgtaacacta tcgttgaccc tggaataggc tttggtaaga
     2701 cgccgcagca caacctggag ataatgaggc gtcttcgtga actcaagagc ctgggaaggc
     2761 cgatcctagt aggaacatcg cgcaagtcca caattggcaa ggttctgggg agacccgtgc
     2821 atgacagagt atggggtacg gcggcaactg tcgcgctggc catcgcatcc ggagccgaca
     2881 tggtgagagt gcatgacgtc gctgaaatgg cgacggttgc ccgaatgacg gacgcggtca
     2941 ctcggggatg gcgcggagag gaggacggca atgcaggctg acaggatcac actgaagaac
     3001 ctggtgttct atgcctacca cggtgtattt gcggccgaga aggaactggg gcagaggttc
     3061 gaggtggata tcgacatgta cggcgacttc cagactgcag cgcgtgccga cgacattgac
     3121 atgaccgtga actacggaga cgtgtacgcg ctggtcaaag agattgtcga ggagcgcgag
     3181 tttgacctca tcgaagcgct ggctggtact atcggcgacg aagtgctggc gggctaccct
     3241 gtgagccgcg tgacagtccg tgtgcgaaag cccaatgcgc ctctgggcgg gcctgccgac
     3301 tacgtggagt gcgagattag ccgcggcgcg gccgggggag gacagccggg gcccagatgt
     3361 gaggacgacg tgtgtgaagg tgaagccgag ctttgacatt ctacaagtag aagagactga
     3421 ttcgacgaac cggatggcac gggtgctggc agaaagcgga gcgtgtgacg gcactgtggt
     3481 ggtcgccgga cggcagacgg cgggtcgcgg gcggcgcggg aggtcctggt tgtcaagtgg
     3541 cgacttcggg ctgtggttct ctgtagtgct caggccggag tttgagccga acctcgcggg
     3601 ccttctaggg attggtgcgg ctgttgccgt tgcccgtgcc gtagacaagc ttaccggagt
     3661 gagcgccgat ctcaagtggc ccaacgatgt gctgataggc ggccgaaagg tctgcggggt
     3721 gctggccgaa gcatcgacag cagccgggcg cgtcgagtgg gccgtggtcg gcgtggggat
     3781 caacctgttt catcctgagg gtgggtttcc accggagata tcgccggtgg ccacctcgct
     3841 ttccgaacac atgggcccgg aggccgcgat cgaccgtgag cgcctgctgg gggcagtcct
     3901 agatgaactg gcggaccgcc ttgcgctact tgagcgggag ggcgcccgcg gcgcttgcga
     3961 gcttgtggac gaggctgagc gcctcatgcg gtccatgatg ggccgccgga taacggtcaa
     4021 ggctgcgtca ggccagtttg aggcggtggc tgtgcgattg gaccacgaag gcggcttggt
     4081 ggtcgcaaac gactgcggcg agtcgatcag agtcacgtcg gccgacgtat caataggata
     4141 tggtccggca gaatcggcag aatcggcaca atcggcggaa ccagcagaac cggcagagga
     4201 ggctcctgct cgatgaacaa ggcaattgct ctagctgtag ctgcgttgat ggtcggcatg
     4261 tcgtgtaggg cgccggcggt tgctgctgcg ccttcggttc cggcagccag gactgccgcg
     4321 gcgcccggac gcctgacgct ggagccgggc tggaccgata tcgcgcggat catcacaaat
     4381 cttgggggtg ccggcgcatc ggttgccctc tccgcggcac ttttagctta cgaccctccc
     4441 acaggcaaga tgatgcttaa tgccaccgcg tgcactgcag gggttgtgga ggcgctcaag
     4501 ataggtttag gtcgcgcgcg gccgtacgtg ccaggtgatg gcggacggtt tacgggccca
     4561 aacctatcgc cggattacca ctccatgccg tcgggtcaca cggctaatgc attcgccatg
     4621 gcgactgtgc tgtcacacaa acaccccgac tggtccccag tgttgtacgg gcttgcgctg
     4681 gcggtgggcc tgagccggat cgagctgggc gtacactggc cgagcgatgt tgtgatcggg
     4741 gccggcatag ggcttgcggt gggtattcag gttactcagg gccgtctgcg cctgtttgcc
     4801 ttcgacaacg attagcattg aatgctgaga acaactgaac cgtggcggcg ccagatttcc
     4861 ggcggccacg gttcgagtgt cttaccgacc tgacatctca gcgatcaatc ctcacctcgc
     4921 gtgggtatgc atcccaggca cggtccacca ggcggctggg gcgcgggcga gggcggaacc
     4981 tcagggaatt ctggaactgg cggcatgggg cccggccccg gcattgtggg gcacggaggc
     5041 tgtgggtacg ggtagtacgg tggacacggg gtcggcgccg gcatcaccgg catgggcatc
     5101 gggcacggca taggcattgg gcatggcatc ggtgttggac atggcatcgg cgttggacat
     5161 ggcatcggcg tcggccatgg ccccgggcat ggcgcaggcg tgggaatcgg ctgcggcatg
     5221 ggagcggggc cagggcacgg catcggagta gggtactgcg caggcgtggg cattatcagc
     5281 ccggggcatg gtgccggctg tgccggcggc cacatcatct cgggcggata catcgggcat
     5341 ggcgcaggtg taggcatcgg catcggcgtt ggacatggca tcgggattgg cgccggcgtc
     5401 ggctgaatgg cctccatcgg cgaacagcag aggatcgtat cgaagaacgt cgcgtgccgg
     5461 agctcgtcgc cgcatatatc gaggatcgcc cgtttcaggc actctgtggg agccagttgc
     5521 gccatcctgc tataggagtg gactccttcg atttcgtctg cataggcatc ctgtaccatg
     5581 gagaagaagt cgttggaagg cagcacgtga tatcacctcg tcatgtgggt tctagcttct
     5641 tcagtggcag tatattgcta gggtcctcgt caagttccca tgccttagtc acttctgcgc
     5701 gattgcggca gctgacagat aatgcggagg tatgccccat ggcagatgag aggcgatcca
     5761 ttgacctatc agtaacggga atgtcatgcg cagcctgcgc tgctcgcgtc gaacgatcgc
     5821 tgaaacacga acccgggatc caccaggcca gcgtcaatct ggcatcggag actgctcgcg
     5881 tgctgtatga tcccggcgtt gtggatccag accggatcat ccaggttgtg gaaaacgccg
     5941 ggtacagcgc tcgtgaacta gttcgtgacc ggcgcgagga cgatgcacgc aaggccgagg
     6001 ataggcggag agcaggcatg aactggggcc tgttctgggt cggcgcggtc ctcagtcttc
     6061 ccatggtcat cgggatgctg gcggaactcg ccggcgtgca ttcgctcatg ttcctgatgg
     6121 accctgtgct cgggtttgtg ctggccacgc cggttgtgct ggtgcccggt tcgcgctttt
     6181 acatcagcgc ttacaagacc gtgacgcacg gcggcgccaa tatggacgtg ctcgtggcct
     6241 tgggcacggg cgcggcctac gtgctgagcg tggtaaacac gttttttgct cgcggtccag
     6301 tctactacga agcggccgcg gttgtgataa ccttggtgct tctagggaag aaccttgagc
     6361 acggcgccaa aggaagagcg agcgaggcca ttcggaaact tgtctctctt accccgcgca
     6421 cagcgacgat tatgcgcggc gaaatcgagg agacggtagc g
//
LOCUS       DAOTAC010000134         6433 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_10216_length_6433_cov_4.806836, whole genome shotgun sequence.
ACCESSION   DAOTAC010000134 DAOTAC010000000
VERSION     DAOTAC010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6433
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10216_length_6433_cov_4.806836"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="PLM74_06860"
     CDS             <1..706
                     /locus_tag="PLM74_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42831.1"
                     /translation="LFPGLSRREVAVTAPFLFRAAELLARVEPDVSFVLCLSPFVGTA
                     SLEQLFSHTRHAARIDGTAARVEKSAGPLEAITTEGIRVRIETVLMYEAMAASDIAVC
                     TPGPVCAELAYLGVPHVVTAPLNVPGALAVPGVSPLLCSLPFLGETFQRRAVDRLKTS
                     SRFVSIVNQRAGRMIAPEIVGRVRPEDVVIPCVELLGEAARRDKMSKELREAAGLTGA
                     ADRVVQAIQRVALSQQ"
     gene            861..1598
                     /gene="lptB"
                     /locus_tag="PLM74_06865"
     CDS             861..1598
                     /gene="lptB"
                     /locus_tag="PLM74_06865"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HON42832.1"
                     /translation="MNDLNGSGLCAAGLAKSFAGRRVVDGACVSVGRGEIVGLLGPNG
                     AGKTTTFYMLVGLEHPDSGRISIGGADITFAPVWERARRGLGYLAQEPSIFRRLTVEA
                     NIMSVLQASGGSPEPCASRLESLLEQFDLARLRRQMGFTLSGGERRRVEIARALAASP
                     CYMLLDEPFTGVDPIAVGELQEIVIGLKQAGLGVLITDHNVRDTLSIVDRAYIMHEGR
                     ILVSGTAQEISEDSAARQVYLGDRFSL"
     gene            1635..2981
                     /locus_tag="PLM74_06870"
     CDS             1635..2981
                     /locus_tag="PLM74_06870"
                     /inference="COORDINATES: protein motif:HMM:NF022727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3084 domain-containing protein"
                     /protein_id="HON42833.1"
                     /translation="MYGVALIVVLAIAGGVIAYAGDRIGMKVGRKRLSIFGLRPKYTS
                     MIITVITGVVIAGCSILLLSLASENVRKALFQIREIQEALSTTQSDLEAKRAEAEMLS
                     RQVGEITGEYEELQVRFDEANRELVRVSEERTRAVGELARVQARLVESEERLRGIEAR
                     FEAAANDLGIANAELESAQAERSRLEQEKAGLQASVASLTLTRDELTREVASLEKKVE
                     DIRVSAAMALAEAAQSSQTSMIGRVIFRADEVVLGRVIDASQPVESIDEQVRAFLLEI
                     NDIAVRRGSGPADDSHRDDGDTSAIRFDLANASAAFAKIMESRDKVVLRAVSPVNTWY
                     GGVLLVSLHVFPEKRIFEAGQVVASRVIDGSAGIDKIQEGLLGLMEDVNSALMAAGMP
                     TDEEGKIGTILTVAEFASAITRVLSNKAPVTVEAVAERDIWRIAGPLSLKLMIKPV"
     gene            3275..>6433
                     /locus_tag="PLM74_06875"
     CDS             3275..>6433
                     /locus_tag="PLM74_06875"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HON42834.1"
                     /translation="MKKFLAILVSAVMIIVMALPVLASPFSDLSDSHWAADAVKMLAA
                     LGIIQGYPDGTFKGRNQATRYEVALMVARALEYLDKDIRDLTERLNQLQGEPVPAQPE
                     PSSGEVVLQAPSPDATILEQVIAEKIAGMSEAQWEEFDDRLSALLARIDDLSEANKAE
                     HADLWKALGARKDGSTGDEDLQKEMELALLQQKAELMMAFYDSIAAANDERDRAIERL
                     KAEFDQALLDQQAALVASFYDVLEAYKGEHEIDHAALEAKIEALLAELKPADGKAAGG
                     ELDQALLDQQAAMMAAIYDVLDAYKGEHEIDHAALEAKFEALCAEQAAALEASFREAL
                     ADLRDEVSVAMLEHKAELTMAFYDSIQALKDEYEQRFQSLGEQWEVALLEQKAELTMA
                     FYDSVQALKDENEQKFAAIEANWERALLEQKAELTMAFYDSIAALRDANEQRFAAIDQ
                     AFVAIEEKWEVALLEQKAELTMSFYDSIEAVKDEYEQRFAAIEDKWGLALLEQKAELT
                     MAFYDSIEAVKDEYEQRFASLEEKWEVALLEQKAELTMAFYDSVAAERDARIRALDEL
                     AAMHEQDLINAKAEMSAATYDALEAVRGEFSLEIDAIMAVIDALRAEYDTELKALGAR
                     IDAVDAALNARIDALEIEVADVRALADQNAFDIVDTYHKIKAEDIAPLTERVDKLEES
                     AAAAHAEIKALRDDFGKVRIGGSNELKFVDIDAHGDVGAAFYKNPFAGSGSVYAPTSK
                     LENTLKLALKLQPRQGVNIDIGLKAVTDVFGGPDTLGLFSGDIDALITTPQDVTKLRA
                     GTLDRPGNFTKYQISAAEFADTEVRGIGAEYKRGTLSASGIIAKLPPTADGTKDFQYV
                     LGAGTQFKLTDKLTLGARVLDIRNDEWSGGVYGSGSRPEEITFGGDFALKLADEFSMT
                     GEMSMWKNSDMDEYLTAHNLGVQGKLGFLNIKASHEQVEEGYDPVFRGTAEGVDPDFL
                     ANDIRLSSLTVETDPMRGFVITGEVSREGDAYYDTEDEAAYGAKVAYNTKLLDADLTL
                     RGGLKHVDVRKPVDDPATTAQLG"
BASE COUNT         1339 a         1708 c         2108 g         1278 t
ORIGIN      
        1 gctctttccg gggctcagcc ggcgtgaggt cgcggtgacg gccccgtttc tatttcgcgc
       61 ggcggagctg cttgcccgcg tggaacctga tgtaagtttt gttctgtgcc tgtctccgtt
      121 tgtcggtact gcctcactgg aacagctctt ctcgcacacc aggcatgccg cgagaattga
      181 cgggacggcg gcacgcgtcg agaagagcgc cgggccgctt gaagcgataa ccacggaagg
      241 catacgcgtc aggatagaga cggtactcat gtacgaggcc atggcggcat ctgacatcgc
      301 agtctgcacg cctggcccgg tctgtgcgga gttagcctac cttggagtgc cgcatgtcgt
      361 gacggccccc ctcaacgtgc ccggcgcgct ggcggtaccc ggtgtatccc cactgctatg
      421 ttcgttgcct ttcctaggag agaccttcca aagacgggcg gttgaccgtc tcaaaacatc
      481 gtcgcgcttt gtgtccatcg tgaaccagcg agcaggcagg atgatcgcgc ccgagattgt
      541 ggggcgcgtg cgtccggagg atgtggtgat cccatgcgtg gaattgctgg gagaggctgc
      601 gcgacgcgac aagatgtcca aggaactacg cgaagcggcc gggctcacag gcgcagctga
      661 ccgggtggtt caggctatcc agcgagtggc gctgtcccag caataaccga cattcgcggc
      721 gcagcttgtg ggcaggtatg tagcatgcgc gtgtcgaaaa atcagtataa tgccgacaac
      781 atctcgcggc gcccgggaac cttgtaatgg cagggtgcgt ctgtaacggg tgaacgctgg
      841 gggttttggg ggcgcctgcc atgaacgatc tgaacggctc gggtctttgc gctgccggtc
      901 tcgccaagag ctttgccggc aggcgcgtcg tcgatggcgc ctgcgtgtcg gtggggcgcg
      961 gggagatagt ggggcttctt gggcctaacg gcgccgggaa gaccaccact ttttacatgc
     1021 tagtcgggct tgagcaccct gattcggggc gcatcagcat tggcggggca gatatcacct
     1081 ttgcgccagt gtgggagcgt gcgcgtcgcg ggctcggtta cctagctcaa gaaccatcga
     1141 tatttcgtcg gctcacagtt gaggccaaca tcatgtcagt cttgcaggca tcaggcggct
     1201 cgcctgagcc atgtgcaagt cggctcgagt cactgcttga gcaattcgat ctggcgcgtc
     1261 tcaggcgtca gatggggttc actttatcag gcggcgagcg caggcgggtc gagatagcca
     1321 gggctctggc tgcatccccc tgctacatgc tgttggacga gcctttcaca ggggtcgacc
     1381 cgattgccgt gggagaactg caggagatcg taatcgggtt gaagcaggct gggctcggag
     1441 ttctgatcac cgaccacaac gtacgggata ccctgtccat tgtggatcgt gcctatatca
     1501 tgcacgaagg gcggatactt gtgtccggaa ccgcccagga gatctcggag gattccgctg
     1561 cacggcaggt ctatcttggt gacaggttca gtctctgagc cggagtcttc cttctacccg
     1621 ggaggggatg tgcagtgtat ggagtagcgc tcatagtggt gcttgcaatc gcgggcggcg
     1681 tgattgcata tgccggcgac agaataggca tgaaggtcgg caggaagcga ctgtccatct
     1741 tcggcctaag gcccaagtac acttccatga tcattactgt cattactgga gtagtgattg
     1801 ccggttgttc catactcctg ctctcgctgg catcggaaaa tgtcagaaaa gcgctgtttc
     1861 agatccgcga aatccaggag gctctatcca caacccagag tgatctcgag gccaaacgcg
     1921 cagaggcaga gatgctgtcg aggcaggtag gagagatcac aggcgagtat gaggagcttc
     1981 aggtcagatt cgatgaggca aatcgcgaac tggtgcgcgt atccgaggag cgtacccggg
     2041 ccgtagggga gcttgcccga gtccaggcac gtctggtcga gtcggaggaa cgcctccgcg
     2101 ggattgaggc aaggttcgaa gccgccgcca acgatctagg catcgccaat gccgagctgg
     2161 agtcggccca ggcggaaaga tccaggctcg aacaggagaa ggccgggctt caggcgagtg
     2221 ttgcttcgct cactctcacc cgagatgagc taactcggga ggtagcgagc ctggagaaga
     2281 aggtggagga catcagggtc agtgcggcaa tggccctggc tgaggcggcg cagtcgtccc
     2341 aaacctcgat gattggacga gtcatattcc gtgccgatga agtcgtgttg ggaagggtaa
     2401 ttgacgcctc gcaaccagtg gaatccatcg atgaacaagt gcgcgcgttt ttgcttgaga
     2461 tcaacgacat agcggttcgc cgtggttccg ggcctgccga cgactcccac cgggatgatg
     2521 gcgatacatc tgccatcagg ttcgacctgg cgaatgcctc ggctgcgttt gccaagataa
     2581 tggagtcgcg cgacaaggta gtattgcggg cggtatctcc ggtgaatacc tggtacggcg
     2641 gtgtgctgct agtatcgctg cacgtgttcc cggagaagcg aatcttcgag gccgggcagg
     2701 tcgttgcatc cagggtgatt gacggttcgg ccgggatcga caagatccag gagggtctgc
     2761 ttggcctgat ggaggatgtc aacagtgctc tgatggcagc gggtatgccc actgacgagg
     2821 agggcaagat cggtaccata cttacggtgg ccgagttcgc atcggcgatt acgagggtgc
     2881 tgtcaaacaa ggccccggtc acagttgagg ccgtggcaga gcgcgacatc tggcgcatcg
     2941 ccggtcctct atccctgaag ctgatgatca agcctgtgtg agcgttccag catctcacag
     3001 cgggcaaaaa tcttacctgt ggtgaaggag ttgaccgcgg tttggagaat atcatcacgc
     3061 aggccgagtt ttagcgatac ctgcgttttc gctctttgac tggcggcgag ttggaaggag
     3121 gcgccgctgc cgaccagagt gccggttctt catactggca atggggctca cttctgctgg
     3181 gcgccggggc gaggaaacta gaacactcgt cagggtgacc ccgttagatg cgagttctta
     3241 gcgttaagca ccaactcgag gggggaagag attaatgaag aaattcctgg ccatacttgt
     3301 cagcgccgtg atgatcatcg taatggcgct accggtgttg gcgagtccct tctctgatct
     3361 gtccgatagc cactgggctg ccgacgcggt gaagatgctt gctgcgttgg gcataatcca
     3421 gggatatccg gacgggacgt tcaagggacg caaccaggca acgagatatg aggtcgcgct
     3481 catggtggcc agggcgctcg agtatctcga caaggacatc agggacctca cggaaaggtt
     3541 gaaccaactc cagggcgagc ccgtgccggc tcaacctgag ccgagctcgg gtgaagtggt
     3601 ccttcaggct ccgtcgcccg acgcgaccat tcttgagcag gtgatagccg agaagatcgc
     3661 cggcatgtcg gaagcgcagt gggaggagtt cgacgatagg ctgtcggccc tccttgcccg
     3721 gatcgatgat ctttccgagg ccaataaggc ggagcatgct gatctatgga aggcgttggg
     3781 ggcccggaag gacggctcca caggtgatga ggatcttcag aaagagatgg agcttgccct
     3841 ccttcagcag aaggccgagc tcatgatggc cttctacgat tcgatcgcgg ctgcaaacga
     3901 tgagagggat cgcgcgatcg agcgcctaaa ggccgaattc gatcaggccc tgctcgacca
     3961 gcaggccgct ctggtggctt ctttctacga tgtgctcgag gcgtacaagg gcgagcacga
     4021 aatcgaccac gctgcgctgg aggccaagat tgaggcccta ttggctgagc ttaagcccgc
     4081 ggatggcaag gccgctgggg gcgagctcga tcaggcattg ctcgaccagc aggccgcaat
     4141 gatggccgcc atatacgatg tgctcgacgc ctacaaaggt gagcacgaaa tagaccatgc
     4201 tgcgctggag gccaagttcg aggccctctg cgcggagcag gctgcagcgc tggaggcttc
     4261 cttcagggaa gcgcttgccg atttgcgcga tgaagtctcc gtggcgatgc tggagcacaa
     4321 ggccgaattg accatggcat tctacgactc cattcaggcg ctgaaagacg aatacgaaca
     4381 gaggttccag tccctcggag agcagtggga agtggcgctt ttggagcaga aggcagaact
     4441 gaccatggcc ttttacgact ctgtgcaggc gctgaaggat gagaacgagc agaagttcgc
     4501 tgctatcgag gcgaactggg agcgggcgct tctggagcag aaggcagaac tgaccatggc
     4561 cttctacgat tccatcgctg ccctacgcga tgcgaatgag cagaggtttg ctgcgatcga
     4621 ccaggcgttc gttgccatcg aagagaagtg ggaagtcgcg cttctggagc agaaggcaga
     4681 actgactatg tccttctacg attccattga ggcggtgaaa gacgagtacg agcagaggtt
     4741 cgccgccatc gaagacaagt ggggactagc gcttctggag cagaaggcgg aactgaccat
     4801 ggccttctac gattccattg aggcggtgaa agacgagtac gagcagaggt tcgcttctct
     4861 cgaggagaaa tgggaagtcg cgcttctgga gcagaaggcc gaactgacca tggccttcta
     4921 cgactcggtt gcggctgaga gagatgcacg catccgcgct ctggacgaac tcgctgccat
     4981 gcacgagcag gatctgatca atgccaaggc agagatgtcg gcagctacct acgatgcact
     5041 cgaagcggta cgcggtgagt tctcactgga gatcgacgca ataatggctg tgatcgatgc
     5101 gctcagggct gagtacgaca cggagcttaa ggccctcggc gcgcgtatcg acgctgtgga
     5161 tgcggcgctc aatgcgcgta tcgatgccct ggagatagag gtggctgatg tcagggcgct
     5221 tgcggatcag aacgcgttcg acatcgtcga cacctaccac aagatcaagg cggaagacat
     5281 tgctccgctc accgagcgcg ttgacaagct cgaggagtcc gcggccgccg cgcacgcgga
     5341 gatcaaggcg cttcgcgacg actttggcaa ggtgcgcatc ggtgggagca atgaactgaa
     5401 gttcgtggat atcgatgccc acggcgatgt tggcgccgcg ttctacaaga atccgttcgc
     5461 cgggtccggt agtgtgtacg cgcctacatc gaaactggag aatacactca agctcgcgct
     5521 gaagcttcag ccgcgccagg gtgtgaacat cgacataggc ctgaaggccg ttaccgacgt
     5581 gttcggcggg cctgatacgc tggggctgtt tagtggggat attgatgcat tgatcactac
     5641 cccgcaggac gtgaccaagc tcagagctgg cacgcttgac cgcccgggca acttcaccaa
     5701 gtaccagatc agcgccgctg agtttgccga caccgaggtg cgcggtattg gcgctgaata
     5761 caagcggggt accttgagcg cctcgggcat tatcgctaag ctgcctccga ccgccgatgg
     5821 caccaaggat ttccagtatg tgctgggtgc cggcactcag ttcaagctca ccgacaagct
     5881 cacattgggc gccagagtgc ttgacattcg caacgatgag tggtcgggcg gggtgtacgg
     5941 ctccggatct aggcctgagg agattacgtt cggcggcgac tttgcgctta agctcgcgga
     6001 cgagttcagc atgaccggtg agatgtcgat gtggaagaac agcgatatgg acgagtatct
     6061 cactgctcac aatctgggcg tccagggcaa gctcgggttc cttaacatca aggcctcgca
     6121 tgagcaggtc gaggaggggt atgatcccgt gttccgcggt accgcggagg gggtcgaccc
     6181 ggacttcctg gccaacgata tccgcttgtc cagtctcact gttgaaaccg acccgatgcg
     6241 gggcttcgtc ataaccggcg aggtatcccg cgagggcgac gcgtactatg ataccgagga
     6301 cgaggcggcc tatggagcga aggtggcgta caacacgaag cttctggacg cggacctcac
     6361 tttaagaggc ggcctcaaac acgtggatgt caggaagcca gtggatgatc ctgccaccac
     6421 agcgcaactg ggc
//
LOCUS       DAOTAC010000135         6426 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_10233_length_6426_cov_3.846335, whole genome shotgun sequence.
ACCESSION   DAOTAC010000135 DAOTAC010000000
VERSION     DAOTAC010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6426
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10233_length_6426_cov_3.846335"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1396
                     /locus_tag="PLM74_06880"
     CDS             <1..1396
                     /locus_tag="PLM74_06880"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HON42835.1"
                     /translation="VKAGAEFLTDSAALGVFPGPRLAVNVGNEEAIAVSAKRILLATG
                     ATENAVSFPGWTLPGVMGAGAAQTMLNVNRVLPGNRILMIGSGNVGLVVSYQLLQGGA
                     AVLAVIEAAPRVGGYAVHANKIARAGVEILTSHVVTEARGVDRVTSAQVAKVDDRWTP
                     IKGTEREFEVDAICIATGLNAMTELARMAGCRIVHLRELGGYTPWHDRRMCTSLPEIY
                     VAGDAAGVEEASSAMEKGRIAGISILEDLGFIAPCDAQTERDDLWKSLEALRQGPFGN
                     TVRRGYDRLFGDESRCSSCTSAHRGEDFMVGSAPRKAPGYPSDTRLAKGPVAVIECTQ
                     EIPCNPCESACARHAITVGDPITSLPRLDEDKCNGCGRCIAYCSGLAIITIDAAYSDT
                     EALMGFPYEFLPCPNKGDEVSLVDRLGKPIARGRVMRVTKPMDGTRVVYAVAPKELIH
                     DIRGIKWQAGGESQ"
     gene            1393..1683
                     /locus_tag="PLM74_06885"
     CDS             1393..1683
                     /locus_tag="PLM74_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HON42836.1"
                     /translation="MSDSKESCLVCRCEELTEDEIRQAIREGCTTLTAIKRRTRAGMG
                     LCQGGTCQRLILAILAQETGQDVAELVPDTSRFPVRPIRAELVGDLRDGEDS"
     gene            1667..2815
                     /locus_tag="PLM74_06890"
     CDS             1667..2815
                     /locus_tag="PLM74_06890"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HON42837.1"
                     /translation="MAKTVDECQVAIIGGGIIGCFIGYYLSARGMDVVVIEKADLCSG
                     ATGANDACLMAQSKKPGPKLDMAVESAHMFETLADELDYDIEYVRKGSMIIQETEEEA
                     QAMREFCTAQKDAGLPVEFLDQRETHRAQPCLSDHIIGSTVCPLDGEVNPFALLVALD
                     RAIRRYGGRIMTNAPVVGFEVAGNRVTEVATDRGNVRADVVVCAAGAYAPLIGNMLRI
                     EIPIRPRRGQILITEPIAPLIGAYVMSAKAIAAKHRAVAVHENNSGKDDPMQFGLGMI
                     ISQTVNGNMVLGVTHEFVGYDAGTSIRGLGSIARYTSTIIPALKGVNVIRTLAGLRPF
                     SEVGGPVIGPIGEYANFIVVTGHEGDGVAVAPSTAKGVADKIASGAWG"
     gene            2943..3953
                     /locus_tag="PLM74_06895"
     CDS             2943..3953
                     /locus_tag="PLM74_06895"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HON42838.1"
                     /translation="MRSAKRIMVCGIALVLAAVASIGFSGYGIAAAPKVVVLQAASGG
                     LGGTWYGQMAALAAIVKEADPGIDMKTVPGAGLANPLRVDSGDAQVAWGMPTFVKDAV
                     EGKGEFNTPSKNVRGIAAGFTLHPLQFLVTRDSGIKSIEEIKQKRLPIRIAIGRAGSV
                     DEITARMILEYYGITYDTVKSWGGSVMLLEYSEQVKKIKDRQADAYILMMPIPAPTVQ
                     EIILNRPLKMLPLDPKMAEEFNRKYAFAVQKLTPDMYDGKTIDEAMIVPAVDLSLIVN
                     NKVSDDVVYRITKAICENVDKVRAIGPTFVDFDPAESWKNTGAPLHPGAEKYYREMGY
                     IK"
     gene            4090..5973
                     /locus_tag="PLM74_06900"
     CDS             4090..5973
                     /locus_tag="PLM74_06900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HON42839.1"
                     /translation="MRTFKGPLATAVLIWALVAASIHIYAVVRVVNTAFLCGVHLLCL
                     LPLGFILYPATPKSPKDRPTAMDVILAALAFACALYLTKNYSYAETRWYLASPVSTLE
                     AVLGAINVLLIVEATRRAVSPVMAVVEIAAIVHLALGQFMPGVLHHPGFSFVRVTEMF
                     YLNRDQGIYGMLTSISSTYIVLFVLFGAFIAQSGVGEFFTDLAFKIAGKSPGGPAKVA
                     VISSALFGTVSGIGTANVYGTGTFTIPLMKKMGYKPEFAGAVEAAASTGGQYMPPVMG
                     AAAFVMAQWIGIPYVKLAMHASLSAILFFVSIGICVDIRARRRNLAAGGDLGVGTKPW
                     SEILKRVYNLLPLAVILGLLLAGYSPMYAGLAAIIVTVAISYFSRDTRMTLGKIIAAL
                     DTGMKNTIMVAIACAGAGIIVASISHTALGLSFVSMVLSLSRGHLLPALLLTALISLI
                     LGTGMPTTPAYILTASLAAPALTQMGVDVVAAHLFVLYFAVISEVTPPVAICAYAGAN
                     IAKADPIKTGVEAMKLSIGGYIVPIAFAFNGAFLLNGNVGGILLASALGVWGLWLVNV
                     GLEGWMFRLISPPMRIVSVALGCACFVPNQLVAIAAALIALGVALAERRRAQGGVSAR
                     ASA"
     gene            6004..>6426
                     /locus_tag="PLM74_06905"
     CDS             6004..>6426
                     /locus_tag="PLM74_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HON42840.1"
                     /translation="MSVVIGGRTNYGISIGILMLQTRFPRLPGDIGNASTFPFPVSYR
                     VVEGACARRVVMEGDPSLIVPFIDAAKGLIAEGVRTVVTSCGFLALFQSELSEALTVP
                     VYTSSLLQVPLVHAITGRRRVGVLTANSDALTDRHFAAV"
BASE COUNT         1360 a         1686 c         2039 g         1341 t
ORIGIN      
        1 cgttaaggcc ggggccgaat tcctcaccga tagcgcggcg ctaggcgtat tccctgggcc
       61 ccggctggcg gtgaacgtag ggaatgaaga agccattgct gtgtctgcca agagaatcct
      121 gctagctaca ggggcgactg agaatgctgt gtctttccca gggtggacac tgcccggagt
      181 gatgggcgcc ggagcagcgc aaaccatgct caatgtcaac cgggtgctgc ctgggaaccg
      241 gattctcatg atcggctccg ggaacgtagg tctggtcgtt agctatcagc tgctgcaggg
      301 cggggccgct gtgttggccg tgatcgaggc ggctcctcgc gtgggcgggt atgctgttca
      361 tgccaacaag atagcccgag ctggggttga gattctcaca tcgcatgtcg ttaccgaagc
      421 gagaggcgtt gacagagtaa cctcggccca agtggcgaaa gtcgacgaca ggtggacgcc
      481 gatcaagggc actgagcgtg aattcgaagt tgacgccata tgcatagcta ccgggctgaa
      541 cgccatgacc gagctggcga ggatggcggg gtgccggatc gttcacctgc gcgaactcgg
      601 cggatacact ccatggcatg accggagaat gtgcacgtcg ttgcctgaga tctacgtggc
      661 cggcgatgcg gcaggagtcg aagaagcaag cagcgccatg gagaagggac gaatcgcagg
      721 gatctcgatc ctcgaagacc tgggtttcat cgcgccatgc gacgctcaaa cagagcggga
      781 cgatctgtgg aaatcgcttg aagctctcag gcagggccca ttcggcaaca ctgtccgacg
      841 aggctacgac aggctgttcg gcgatgaatc caggtgcagc tcgtgcacgt ctgcgcatcg
      901 gggcgaggac tttatggtcg gttcagcccc gagaaaggca ccgggttacc catcggacac
      961 gaggcttgcc aagggaccgg ttgcggttat cgagtgcacc caggagattc cgtgcaatcc
     1021 gtgtgagtca gcttgtgcaa ggcacgcgat tactgtcggt gatccgatta ccagcctgcc
     1081 caggctggat gaggacaagt gcaacggttg cggacgttgc atagcatact gctccgggct
     1141 cgcgatcatc acgattgatg cagcctattc tgacacagag gccctgatgg gcttcccata
     1201 cgagttcctg ccctgcccga acaagggcga cgaggtttcc ctagtggaca ggctaggcaa
     1261 gccgattgct cgaggtcgag taatgcgtgt caccaaaccc atggacggta ctcgggtggt
     1321 gtacgctgtc gcgcccaaag agctgattca cgacatccgg ggcatcaagt ggcaggcggg
     1381 aggcgagagt caatgagcga cagcaaggaa agctgcctag tatgccgttg cgaagaactg
     1441 actgaagacg agataaggca ggctatcagg gaaggctgta ccactctcac ggccattaag
     1501 cggcgcacgc gcgccggcat ggggctgtgc caggggggga catgtcagcg tcttatcctg
     1561 gcgattcttg ctcaggaaac gggccaagat gtcgcggagc ttgtgccgga cacgtcgagg
     1621 ttccctgtca ggccaatacg cgctgaactg gtaggtgatc tgcgagatgg cgaagacagt
     1681 tgacgaatgc caggttgcca taatcggtgg agggattatc ggctgcttca tcggctacta
     1741 cctttcggcg cgcggcatgg atgtagtagt gatagagaag gccgacttat gctcaggcgc
     1801 taccggtgcg aacgatgcat gcctgatggc ccagtccaag aaacccggcc ccaaactgga
     1861 catggcagtt gagagcgccc acatgttcga gacgctcgcc gacgaactcg actatgacat
     1921 cgagtacgtc aggaaaggat caatgatcat tcaggagaca gaagaggaag cccaggcgat
     1981 gcgggagttc tgcaccgcgc agaaggatgc agggctgcct gtggagtttc tagaccagcg
     2041 cgaaacccat cgcgcgcagc catgcctgtc agaccacata atcggatcaa cggtgtgccc
     2101 cctcgacggc gaggtcaacc cgttcgccct actcgtggcg cttgaccgcg ccattcgcag
     2161 atacggcgga cgcatcatga ctaatgctcc tgtggtcggc ttcgaggtgg ccggcaacag
     2221 agtaaccgag gtggctacag accgcggaaa tgtcagggcg gatgtcgttg tgtgcgcagc
     2281 gggcgcctat gccccgctca tcgggaacat gctgagaatc gagataccca tacggccgag
     2341 acgaggccag atactaatta cagagcccat tgcgcctctg attggcgcct acgtgatgtc
     2401 tgccaaggcc atcgcagcta agcatagagc agtcgctgta cacgagaaca attccggcaa
     2461 agacgatccg atgcagtttg gccttggcat gattataagc cagacagtga atggcaatat
     2521 ggtattgggg gtgacacatg aattcgtagg gtacgacgca ggcactagca taaggggtct
     2581 gggctcgata gccagataca cgtcgactat cataccggct ctgaaaggtg ttaatgtgat
     2641 tcgcaccctg gctggtctgc gccccttctc tgaagtggga ggtcccgtaa tcggccccat
     2701 tggggagtac gccaacttca ttgtggtcac tgggcatgaa ggcgatgggg tggcagttgc
     2761 gccttcgacc gcgaagggag ttgcagacaa gatagcgagt ggagcatggg ggtaggccaa
     2821 ggcccatgtg accgtattcg aagcctagac gcggacgtta ctcgtcatca ccacctgtca
     2881 cagttgaact aggatagtga gcccatctcg gtactactca agtcaggtag gaggtaagca
     2941 agatgagatc agccaagcgt atcatggtat gtggtattgc gctggttcta gcagcagtag
     3001 cgtcgattgg gttctcggga tacggtattg cggcagctcc caaagttgta gtactgcagg
     3061 ctgcgtcggg tggcctgggc gggacttggt atggacagat ggcggcactg gccgcgatag
     3121 tcaaagaggc ggatccggga atcgatatga agacggtccc tggggcgggt ttggccaacc
     3181 ccttacgcgt ggatagtggg gacgctcagg tggcatgggg catgcccacc ttcgtgaaag
     3241 acgcggttga aggcaaggga gagttcaaca caccttccaa gaacgtcagg ggcatagctg
     3301 cggggtttac gctccatccc ttgcagttcc tggtgacgcg agacagcggg atcaagtcaa
     3361 ttgaggagat caagcagaag agactgccta ttcgaattgc catcgggcgc gcagggtcag
     3421 tcgacgagat tacagccagg atgatcctgg agtactatgg gatcacctat gacacggtca
     3481 agtcatgggg cggatctgtc atgctcctgg aatacagcga gcaggtgaag aagataaagg
     3541 accgtcaggc agacgcctat atactgatga tgccaattcc ggcgcctacg gttcaggaga
     3601 tcatactcaa ccgaccgctg aagatgctac cgctcgaccc caaaatggct gaggagttca
     3661 accggaagta cgcttttgca gtccagaagc tcacccctga catgtatgat ggcaagacca
     3721 tcgacgaagc gatgatcgtt cccgccgtcg atctctcgct cattgtgaac aacaaggtaa
     3781 gcgatgatgt ggtgtaccgg attaccaagg ccatatgcga gaacgtcgac aaggtccggg
     3841 cgattgggcc gacgttcgtc gatttcgacc ccgcggaatc gtggaagaac accggggcac
     3901 cgctccatcc gggcgcagag aagtactacc gcgagatggg gtacatcaag tagaggctac
     3961 cagtagctcg aatcatgcct tccacgggcc ggagccgggg accggcgtct ccggcgccgg
     4021 ctcccgagga tgtgctgaag acgagtgcga aatgcagttg cggcagagta gtacgagggg
     4081 gtaggaattg tgagaacctt taaggggccc ctggccactg cagtcctgat atgggccttg
     4141 gtggctgcat cgatacacat ttatgcagtt gttcgcgttg tgaatacggc tttcctatgc
     4201 ggagtgcact tgctgtgttt gctgcctctc gggttcatct tgtacccggc tacgccgaaa
     4261 agcccgaaag accgccccac cgccatggac gttatcctgg cagctctagc cttcgcgtgt
     4321 gcactctacc tgaccaagaa ctactcgtat gcagagacca ggtggtacct ggcatcgccc
     4381 gtgtcgaccc tagaagccgt gctgggcgcc ataaacgtac tactgatcgt agaggctaca
     4441 cgtagggcgg tcagccctgt aatggctgtg gtagagatcg ccgccatagt ccatcttgcg
     4501 ctcggtcagt tcatgcccgg cgtcttgcac caccctggct tctcatttgt acgagtgacg
     4561 gagatgttct atctcaatcg cgaccagggt atctatggga tgctgacaag tatctcgtcg
     4621 acgtacattg tgcttttcgt tctgttcgga gctttcattg cgcagtcggg agtaggcgag
     4681 ttcttcaccg acctggcgtt caaaatcgcc ggaaagtctc ctggcggacc cgcgaaggtt
     4741 gcagtcataa gcagcgcttt gttcggcacc gtaagcggga ttggaacagc aaacgtgtac
     4801 ggaacgggca cgttcaccat tcccctcatg aagaagatgg gttacaagcc ggagttcgca
     4861 ggcgccgtag aggcagcggc atctaccggc gggcagtata tgcccccggt gatgggcgct
     4921 gcggcgttcg tgatggcaca gtggatcggc ataccctatg tgaagctagc catgcacgcc
     4981 agcttgagcg ctatactctt cttcgtctcc ataggaatat gcgtggatat cagagctagg
     5041 cggagaaatc tggctgcggg cggggatctt ggggtaggaa ccaagccctg gagcgagatc
     5101 ttgaaacgcg tctacaatct ccttccgcta gcggttatcc ttggcttgct gctggcggga
     5161 tactctccga tgtatgctgg gctagcggcc ataatagtca cggtagccat aagctacttt
     5221 agccgcgaca ctaggatgac cctggggaag attatcgcgg cgcttgacac aggcatgaag
     5281 aacacgatta tggtggccat cgcatgcgcg ggggcaggca taatcgtagc ttcgatctcc
     5341 cacaccgcac ttggtttgtc cttcgtgtcg atggtccttt ccttgtctcg cggtcacttg
     5401 ttgcctgctc tgcttctgac ggccctgata tcgctaatcc tcggcactgg aatgccgacg
     5461 acccctgcgt acatcctcac cgcgagctta gctgcgccgg cgttgactca gatgggagta
     5521 gacgttgtgg cggcccacct atttgtcctg tatttcgcgg tcatctccga ggtcactccg
     5581 cctgtggcga tctgtgcata tgcaggcgcc aacattgcga aggccgaccc gataaagacc
     5641 ggtgtggagg ctatgaagct gagcatagga gggtacattg ttccgatcgc cttcgcattc
     5701 aacggtgcgt ttctgctcaa cgggaatgtc ggtgggattc tgttggcgtc ggcgctgggc
     5761 gtatggggac tgtggctggt gaacgtgggt ctcgagggct ggatgttcag acttatcagc
     5821 ccgccgatga ggatcgtaag tgtagccctg gggtgcgcat gttttgtccc gaaccagttg
     5881 gtcgcgatcg cggcggcgct cattgccttg ggtgtggcac ttgctgagag gcggagggct
     5941 caaggcgggg tctccgccag ggcatcggcc taggcgagat acgcacatgg gaggaacgtt
     6001 gccatgagtg ttgtcatagg aggtcggacc aactacggca tttcgattgg aatactgatg
     6061 ctgcagacca gatttccgag gctgccgggt gacattggta atgcgtctac attcccgttt
     6121 ccggtgtcgt accgggtagt cgaaggggcg tgcgctcggc gcgttgtgat ggagggcgat
     6181 ccttctctca ttgtgccgtt catcgatgca gccaaggggc tcatagccga aggggttcgg
     6241 acggtcgtca cgagctgcgg ctttcttgcc ctgtttcaga gtgagctgag cgaggccctc
     6301 acggttcccg tgtacacctc gagcctgctg caggtgccgc tggtgcatgc gataactggc
     6361 cggcggagag tgggcgtgct cacagcgaat tccgacgccc ttacggatcg gcattttgcc
     6421 gccgtg
//
LOCUS       DAOTAC010000136         6416 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_10245_length_6416_cov_5.118849, whole genome shotgun sequence.
ACCESSION   DAOTAC010000136 DAOTAC010000000
VERSION     DAOTAC010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6416
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10245_length_6416_cov_5.118849"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            983..3229
                     /locus_tag="PLM74_06910"
     CDS             983..3229
                     /locus_tag="PLM74_06910"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HON42841.1"
                     /translation="MFNRLRLGAKLAAVFILVSLIPLSLVSVFAYTQFRGAMKVQVHA
                     AITAYSHSVREQLDSWIAKQRNDATVLSTTRDLREALAYLESADWDVNSTDWLQMKTD
                     ILDPLGDVIAKEYGYAFVFIMDPNGTGVWSNAKVIEGADLSTRDYFQGAIKGIVAYSE
                     IFYSTVVTDNVIVTGSPIYEAGSRRIVGALGLAMSSSELERKLQAGLESLGKSADAYL
                     VAADGTFLTNTKHGIYSKDAVLKQKIDTEAIRLLAEEIASQNVNWTQSLEYPDHDDHV
                     ALSAISVVMLGDHLAGLIVELDTAEAFAGANRMPKTLVVGVGLAAAVVAVIGLFFGRR
                     LANPLLTTASMLKEIASGEGDLTRRLEVTAGDEIGDVAKWFNMFVEKIQAVVKKVQLS
                     SEEVTEASVRLADVAQEVGSASLQVTSAIEQVALGTGEQGKMSGDAAQMMSELSHVID
                     SISRGAETQGKSAGNMASAVVSISETLARSQKAVEKTSDHLKHAATSANDGVRTVHMT
                     SDGMASISRTVSEAAKQIQELGRHSAQIEGIVLAIQDIAEQTNLLALNAAIEAARAGE
                     HGRGFAVVADEVRKLAEGAGRSAREIESLIKEITGSIGHSVESMNASVREIENGVARS
                     AETQQALTRISEQVKSTEAQMSELQAAFEDLAKAQAVLTEAADSIVDITESNSAGAEQ
                     MAAQSTQVADAVSSIASIIEETAASAEEVSASAEEQHAAVEEVATSATRLAELAADLN
                     SMVGGFKA"
     gene            complement(3423..5600)
                     /locus_tag="PLM74_06915"
     CDS             complement(3423..5600)
                     /locus_tag="PLM74_06915"
                     /inference="COORDINATES: protein motif:HMM:NF039707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HON42842.1"
                     /translation="MQHTLGNDRFEIMIDDEACRIARLRNAITGDDYIKTGLNYSICR
                     LVCIDEASGARLEVTPLCAHVCSHAQAPCAQASADRLDTAERTEIEYEACEVVCDCAN
                     GRSASLLGARFDIRVSVRAQARPGNAESVWSVTVANNQPGVRVIQVLFPNLHGVYLGD
                     SWRDDVMIYPHHAGERTQAPIEEYTTPRYLGFGRAQTVRSCDGVFSREINYCGLASMN
                     WMYYYDAQNGLYLASYDPEFVVTGLRVETGGPDCPDGPWMGWAFRKHVEVRTGETWES
                     PPYGIAVTCDDWRWGAKRYRQWADTVMPMPDNPDFLEGEYVLNQCYNFKRDGVIFNKF
                     CDIPRMYEDGRARFGARHMFIASWNRKGFDQNYPEYYPDMELGTAWELAEGCKYVNEH
                     GGFVTFYINSRIFEKTSDYFDSLGVKWAIKDENGEMIHESYGPHDFVVLCPSHDEWKN
                     YLMDVAGWMAKCSHATGIYLDQLGSAEPWPCFDPEHTHASIGEFNRGYLGLVKDLLVR
                     LRSMNRDSFLMIENCGDIYGSYVWGNLTWNGEPYDEFFNLYKYTFPEYVQVNMVNPRR
                     ELDGPDRAQRLHRHLNRALMLGSVFWLGLDKMPYLDDDTREYLDAALRLRARLTPLIR
                     GARYVDMDGVLSVSDGIEVGHWEMADDRDLYIVANPGRVTDGMVVVPTGQGAGNADSD
                     AYKHMVVDDFDIDGGSGRVRYEPCSEELKIRLHDAEVVCILTK"
     gene            complement(5796..>6416)
                     /locus_tag="PLM74_06920"
     CDS             complement(5796..>6416)
                     /locus_tag="PLM74_06920"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HON42843.1"
                     /translation="LVTKEGQSLLGKAESIEALKFLQSLCVVDKAVPTETQAQQYGKA
                     NTAPFIAGKVAMLSGGLSVSVDLINNKVDYEVRPLPKGKNRTNIAFVNAWAVPAGAKR
                     PDLSWRVLKFFASKEAQQIALGTNMGLPARKDVDVSTFVKARPDNKYFIDSLAESKPF
                     PAPLRGVDFFKLIEKEFDMMWLGERSVDDAVRAVEKKAADVLAGKM"
BASE COUNT         1380 a         1899 c         1817 g         1320 t
ORIGIN      
        1 cagcccccag ttctgcgttt gcccttctgg cgacagagct tctgtcagat ccactcaccc
       61 agagcacctc catcacgtgc tgcttcccga cttcagtcta tcgaactcag gcttcctttc
      121 gcttgtcaca gaatctacaa cagatgctac cacacatgcg cgaccaacgt gcgtcgaaag
      181 caaggggtat ggcagtagcg cagtgggcag tcctgcaaca ctgcgcatgc agcaaccggg
      241 caatgcgcac aatcgcgtaa cgcagggcag aattcgcgct gcattagggt aaagcctcta
      301 gatcatcgaa tactctttag gttatcgcaa gacttctgcc gttcatctgg catggctggg
      361 atgcaccatg aaatgccgtg gggttctcag cgcagcgcag cccatgccct gtaggttttc
      421 cgacaggcct ttggcggagc ctgtcggaat accctacggt tctgaacgca ttagccttcc
      481 cgatcgcaac cggctgcggc gcttactttc gtaggcggtc agcattagca gaacgtgcag
      541 ctagagccag tgcccgaatg ccgtgtttcg gtggtgggcc accggagact gccatcccag
      601 agtctgtgat ccccagaacg ccctcagatt tcgcccgaac gtgctgaacc atgcggtatt
      661 ccattaggcc ggcgacaaga ccgtagggtt ttcccacagg cctgacaaag ccttgcccaa
      721 cccggcctta gcggtttgga gcatgtcgcg ccggaaacca cacggcactg caaaatggat
      781 cgagctggct agtctagtgt tctaagtgca gaagttctgt tatcgaacac ctttaggttt
      841 tcgaacgcct ccggaagacg cagatattgc gcccgccaag caccaacatc agctttaggg
      901 cttatccccg aaaactggtt aatccaaagc tcgattcatg tcgatatttc tctaacagca
      961 taacgacagg gaggaacacg ccatgttcaa cagactaagg ctcggggcca aactcgctgc
     1021 cgttttcatc ctggtttcgc ttatcccgct ttcgctggta tcagtgtttg cctacacgca
     1081 gttccggggc gctatgaagg tgcaggttca cgcggcgatc accgcatatt cccactcagt
     1141 gcgagagcag ctcgactcat ggatcgccaa acaacgcaac gacgcaactg tactgtcgac
     1201 taccagggat ttacgagaag ctctggcata tctggagtcg gcggattggg atgtaaacag
     1261 cactgactgg cttcagatga agacggatat attggacccg ctaggtgatg tgattgcaaa
     1321 ggagtatggg tatgcgttcg tgttcatcat ggatccgaac gggactggag tatggtcgaa
     1381 cgctaaggtc atcgaaggcg ctgatttgtc gacccgcgac tacttccagg gcgccataaa
     1441 gggcatagtc gcgtattcgg agatcttcta ctctactgtg gtaactgaca atgtcatcgt
     1501 caccggatct cccatatacg aagcagggag taggcgcatt gtgggggctc tgggcctcgc
     1561 gatgagcagc tcggaactgg agcgcaagct tcaggccggg ctcgaaagcc tgggcaaatc
     1621 agcagatgca tacctagtgg ctgccgacgg cacgttcctc actaacacca aacacggcat
     1681 atactcgaag gatgcagtgc tcaagcagaa gattgatacc gaggccatta ggctgctcgc
     1741 agaggagata gcatcgcaga atgtaaactg gacccaatct cttgagtatc ccgaccatga
     1801 cgatcatgta gcattgtctg cgataagcgt agtgatgcta ggtgaccatt tggcgggact
     1861 gatagtagaa cttgataccg ctgaggcttt cgcaggcgca aacaggatgc cgaagacact
     1921 cgtagtcggc gtcggtttgg ccgccgcggt tgtggccgtg atcggactat tcttcgggcg
     1981 gcggctcgcg aacccgctgc ttacgacggc atcgatgctg aaggagatcg ccagcggcga
     2041 gggcgacctg acgaggcgtc ttgaggttac agccggcgat gagatagggg atgtagccaa
     2101 atggttcaac atgtttgtgg agaaaatcca ggcagtcgtg aagaaggtgc aactcagttc
     2161 ggaggaggtt actgaggcca gcgttcgcct ggctgatgtg gcccaggagg tcggcagcgc
     2221 cagccttcag gtcacgtccg caatcgaaca ggtcgcgctg ggcaccggtg agcagggtaa
     2281 gatgtcaggc gacgcggcgc aaatgatgag cgagctttcg cacgttattg actcaatctc
     2341 ccgaggcgca gagacacaag gcaaatccgc tggcaacatg gcgtcagctg tcgtaagcat
     2401 atcggagacc ttggccagat cgcagaaggc ggtcgagaag acatcggacc acctcaagca
     2461 tgccgctacg tcggcaaacg acggcgtcag aacggtgcac atgacttcag acggcatggc
     2521 ctccataagc cgtaccgtat cggaggcggc caagcagatt caggaactcg gacggcattc
     2581 cgcccagatc gagggcatcg tactggctat acaggacatc gcggaacaga ccaacctgct
     2641 ggccctgaac gctgccattg aagctgcgcg agccggggaa catggcagag gtttcgcggt
     2701 ggtagcagac gaagtcagga aactcgccga gggcgccggc aggtccgccc gagagatcga
     2761 gtcgttgatc aaggagataa ccgggagtat cgggcattca gtggaatcga tgaacgcgag
     2821 tgttcgcgag atcgagaacg gtgtcgcccg ctctgccgaa acacagcagg cactcaccag
     2881 gatatctgag caggtaaaga gcaccgaggc gcagatgtcc gaactgcagg cggcgttcga
     2941 ggacctggcg aaagctcagg ccgtgcttac cgaggcagcc gacagtatcg tagacattac
     3001 cgagtccaac tccgccgggg cagagcagat ggcagcgcag agcacacagg tcgcagacgc
     3061 agtgtcgtcc atagcatcca tcatcgagga aacggcagct agcgccgaag aagtgtcggc
     3121 atcggctgag gaacagcacg cagcggttga ggaggtcgcg acatcagcca cgaggctggc
     3181 cgaacttgcg gcagacctta actccatggt gggtgggttc aaggcgtagg attcggcgcc
     3241 gtagcagagc aaatcgcagc attctgcacc gctgtggcag agcaagaggc cgtactcgca
     3301 caaaagggct ggaatacaga gcgtattggt atcggtcttc gcgtgaactc gacacgaggc
     3361 ccggagatgc accaacctgc acccgggccc cgctcatccg ggcctcgctc tctcaaatac
     3421 cgctacttgg tcagaatgca cacgacctcg gcgtcatgaa ggcgtatctt gagctcctcc
     3481 gaacatggct catagcgcac gcgcccacta cctccgtcga tatcgaaatc gtccacgacc
     3541 atatgcttgt aggcgtccga atcagcgttc cccgcgcctt gcccggtcgg aactactacc
     3601 attccatccg taacgcgccc gggatttgct acgatgtaca gatcccggtc gtctgccatc
     3661 tcccagtgcc cgacctcaat tccatccgag accgacagta cgccgtccat atccacatac
     3721 cgggcccccc ggatcagcgg tgtcagtctg gcccggagcc ttaaggcggc atccaggtat
     3781 tcgcgtgtgt cgtcgtctag gtacggcatc ttatcgagcc ccagccagaa gacagaaccc
     3841 agcatcaatg ctctgttcag gtggcgatgc aggcgctgcg ctctgtccgg cccatccaac
     3901 tctctcctgg ggttgaccat gttgacctgg acatactcgg ggaatgtgta cttgtacaag
     3961 ttgaagaact cgtcgtacgg ctcgccgttc caggtgagat tgccccacac gtagctgccg
     4021 tagatgtcgc cgcagttttc gatcatcagg aagctatccc ggttcatcga ccttagccta
     4081 accagcagat ccttgactag gccgaggtaa ccgcggttga actcgccaat ggacgcatgc
     4141 gtatgctctg ggtcgaagca tggccagggc tccgctgaac ctagctggtc caggtagatc
     4201 ccggtcgcat gcgagcactt agccatccat ccggccacat ccattaggta gttcttccac
     4261 tcatcgtggg atgggcagag cacgacgaaa tcgtgcgggc catacgattc gtggatcatc
     4321 tccccgtttt cgtccttgat ggcccacttc acgcccagcg agtcgaagta gtctgacgtc
     4381 ttttcgaata tgcgcgagtt gatgtagaag gtgacgaacc cgccgtgctc gttgacgtac
     4441 ttgcagccct cagccagttc ccacgctgtg cccagctcca tgtcgggata gtactccggg
     4501 tagttctggt cgaatccctt acggttccag gaggcgatga acatatgcct agcgccaaac
     4561 ctggcgcgcc cgtcttcata catccgcggg atgtcgcaga acttgttgaa gatgacaccg
     4621 tctcgtttga agttgtagca ctgattcagg acgtactcgc cctcaaggaa atccggatta
     4681 tccggcatcg gcataactgt atccgcccac tgcctgtaac gcttagcacc ccaccgccaa
     4741 tcgtcgcatg ttacagcaat gccgtacggc ggcgattccc aggtctcgcc ggtccgcacc
     4801 tccacatgtt tgcggaacgc ccatcccatc caagggccgt ccgggcagtc gggtcccccc
     4861 gtttccaccc gaagcccagt cacgacaaac tcagggtcgt aggaggccag atataggccg
     4921 ttctgagcgt cgtaatagta catccagttc atcgacgcaa gtccgcaata attgatctcc
     4981 ctggaaaaca cgccatcgca actgcgcacg gtttgcgcgc gcccgaagcc cagataacgt
     5041 ggggtggtgt actcctctat cggcgcctgc gtcctctcac ccgcgtgatg cgggtagatc
     5101 atcacatcat cgcgccagct atcccccagg tatactccgt gcagattcgg gaagagcacc
     5161 tggatcacgc gcaccccggg ctgattgttc gcgacggtaa ccgaccacac actctcggca
     5221 ttgcccggac gcgcctgagc ccgcacgctc acacggatgt caaaacgtgc gccaagcaac
     5281 gacgccgagc ggccgttcgc gcagtcgcac acgacctcac acgcctcgta ctcgatctct
     5341 gtcctttctg cagtatccag gcgatcagcg ctagcctgag cgcagggggc ctgagcatgc
     5401 gagcaaacgt gagcgcacag cggcgtcact tcaagccgcg cgccgctggc ctcgtctatg
     5461 cacacaagcc ggcatatgct gtagtttagc ccggtcttga tgtagtcgtc gcctgtgatg
     5521 gcattgcgaa gccgcgctat cctgcatgcc tcgtcgtcga tcatgatttc gaaccgatca
     5581 ttgcccagtg tgtgctgcat gtgctgtgcc tcccctgcag tctgcgcgta atggcgctat
     5641 ggcgaggagg gggtatggcc ccctcctcac tctcagtctc gtcgcggtca cgtccccagt
     5701 caagtctcta ggagtatccc agggaaaacc gctgggaacg ttagtcgcgt tggccgttgc
     5761 cttcttggct cagtctctaa tcaggttgcg cgtatctaca tcttgcctgc cagcacgtcg
     5821 gcagccttct tctcgacggc gcgcactgca tcgtcaacac tgcgctcgcc aagccacatc
     5881 atgtcgaact ccttctcgat cagcttgaag aagtcaacgc cacgaagcgg cgccgggaag
     5941 ggcttgctct cggcaagcga gtcgatgaag tacttgttgt cgggcctggc tttcacgaaa
     6001 gtggacacgt caacgtcctt cctcgccggc agccccatgt tggtgcctag cgcgatctgc
     6061 tgggcttcct tgctcgcgaa gaacttgagc acgcgccacg acagatcggg tctcttcgcc
     6121 cccgccggga ctgcccaggc gttcacaaac gcgatgttgg tgcggttctt gcccttaggt
     6181 agcggcctga cctcgtaatc aaccttgttg ttgatcaggt cgacactgac cgagagtccg
     6241 cctgatagca tcgcgacctt gcccgctatg aagggcgcag tgtttgcttt gccgtactgc
     6301 tgagcctggg tctcggtggg tacggctttg tccacgacgc acaggctctg gaggaacttg
     6361 agcgcctcaa tactttcggc cttgcccaga agggactgtc cctccttggt caccaa
//
LOCUS       DAOTAC010000137         6388 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_10299_length_6388_cov_4.553292, whole genome shotgun sequence.
ACCESSION   DAOTAC010000137 DAOTAC010000000
VERSION     DAOTAC010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6388
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10299_length_6388_cov_4.553292"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..611
                     /locus_tag="PLM74_06925"
     CDS             66..611
                     /locus_tag="PLM74_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HON42844.1"
                     /translation="MNAIGVVELISVARGYAAADAMLKAANVQLHAAKPVCPGKYAIL
                     VYGGVAEVQSSVEAGVEAGGQFVVDQLVIANIHQSVWPAVMGVLPPPAIRALGIIETY
                     SIASTVLAADAAVKAASVALVEVRLALGMGGKAFTTVTGEVSDVAAAVEAATMLAGDR
                     GMLTEAVVIPAPAAGLTEFTI"
     gene            629..958
                     /locus_tag="PLM74_06930"
     CDS             629..958
                     /locus_tag="PLM74_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42845.1"
                     /translation="MPASLLITAAELAGRKAGDRITVPKGAIVTPAARDYASEHGICM
                     VSEDGFVIAQGRPGTHEDDASRIVRGILGELRRELGRVPTRDEAVGFVRAVIERARQE
                     ATDVIDS"
     gene            982..1473
                     /locus_tag="PLM74_06935"
     CDS             982..1473
                     /locus_tag="PLM74_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HON42846.1"
                     /translation="MGAIEAIKGRRSVRRYLPNPVPREIIEDIVDCGRLAPSARNDQP
                     WEFVVVTDQTIREEIAAAATYGKFIADAPVCVVAFARQTRRHTEDTCAAVENMLVAAY
                     AHGLGSCWIAGENTPYEKEIARICKAPDGLRLVALFPLGYANEKPRPNKRDLEEVLHW
                     EGF"
     gene            1436..3226
                     /locus_tag="PLM74_06940"
     CDS             1436..3226
                     /locus_tag="PLM74_06940"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HON42847.1"
                     /translation="MTWKRYSTGRGFRQRVSRLAAFAALILAAATGIAAAVVLFEMRM
                     PAHLASALSGAEPGDAAVADLCVYVQRCSHLGAPDLPAQRVQSLLERRTARDRDGATL
                     VTSPSRASLAREAHQLVLSENGGEWESGFHRSRLERIARVLAMAAGDRPESYTLTLLD
                     TTQTNAVSTADGRIYITRGLATTSTDDEIAMVLAHEMHHIRSGHWYSWWAPDARDVGN
                     GGNSGNGSGEGAPPDPVSEAAQALADLGVDALSGAATTSYAQEFEADARGVLLASMCG
                     YDGRQMYNVLTRLPGMPVTSHPSVAHRIAGVAKVLDAMEDPEWISSHNPVEVAVRSVE
                     AAMRREVAYRPGVSGEAPGLPDVARACQATLDWLNATEAEMIGFRWLGAGRAVSGRRR
                     VLGRTIMIASSLSIVDIAVDTGFVRATAGKPGVVCGRTWLIRRRGVWEPVAAQVLPRT
                     RFAAPEWLKFKDELRGSVAQGPKDVDRDVLRTAQMWRDTAVSDFPGHLTVYARGRLES
                     SAAADSSSRHDNGHGQTLEAIGWARFLGGRQAELRAWSVRCEAHSPTLATVAFSSGLT
                     VDGVPISSNSTRLTLVLEDGQWKVAQVNLD"
     gene            complement(3213..3896)
                     /locus_tag="PLM74_06945"
     CDS             complement(3213..3896)
                     /locus_tag="PLM74_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42848.1"
                     /translation="MRSDEQMDAMEELQTGEDWAIVRALKSGATPAPEFRARLKQSFL
                     LEAESDARRRKAYKLSILGLPRFYTNLVAVGAAAALILMAFTYSIWLKPGPAPQIAEE
                     SPSVLLGPGSGALGFAPNAGDTADAVYVGYSTPLTDEIPDTLELIAAEAGGAVKLPGA
                     GDIAGNIAVHSARHSAGEAQAIVLTVPSDVLDHVLGRLDEAIGTATISRPVHLNSEQV
                     TIYITFSPN"
     gene            complement(3883..4479)
                     /locus_tag="PLM74_06950"
     CDS             complement(3883..4479)
                     /locus_tag="PLM74_06950"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HON42849.1"
                     /translation="MYSDASDEQLIQLHLSGDPRGMEAIYERYFDKVYRLAYSKLRTA
                     QDAEDVTSAVFLKLCKSLDSFRGEAKFSTWIYTVANNAVMDSIRRRRPSVSLDADLRV
                     DGGDSVPREIEDDAPGPEDHVADADFARYVYGKLNALPPGQRSVIELRYFMELSYQQI
                     ADQLGVELGTVKSRLNRAIAALRSLVSTEEEVKASAVR"
     gene            4734..5027
                     /locus_tag="PLM74_06955"
     CDS             4734..5027
                     /locus_tag="PLM74_06955"
                     /inference="COORDINATES: protein motif:HMM:NF025386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HON42850.1"
                     /translation="MGLRFRKSIRIGKFARINISKSGVGASVGVPGFRVGMGADGKVR
                     RTMGIPGTGVYHTEVLASPKNEATKPKRTCQACGRQVAQKDRFCRHCGARLAT"
     gene            5308..>6388
                     /gene="mtnA"
                     /locus_tag="PLM74_06960"
     CDS             5308..>6388
                     /gene="mtnA"
                     /locus_tag="PLM74_06960"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR00512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HON42851.1"
                     /translation="MAGDGGREGGAGRRLVPIAWDATRGAVRLIDQTLLPTELKYIDI
                     RSFDDMCEAIAMLRVRGAPAIGVAGALGIYLGLVERAPVSAAGVFEALDELCPRMIGV
                     RPTAVNLSWAVNRVRQAVERAAVAADAAGAADGAQPPRSYGLLAQCGSLELLRPHELL
                     AAALHEAMVIADEDAAACRRIGEYGSQLIPDGGRVLTHCNAGALATAGWGTALAALYV
                     AQEQGKRFRVWADETRPLLQGSRLTAFELAEAGIDVTVICDNMAASLMRAGQVDIVIV
                     GADRVAANGDVCNKIGTYGLACLANMHRIPFFVACPTSTLDLSLRAGDEIPIEQRDPR
                     EVTHGFGRQTAPDGVGVYNPAFDVTP"
BASE COUNT         1198 a         1736 c         2176 g         1278 t
ORIGIN      
        1 tcccgcatcg tagcgaggtt gggccttgca gagtatgcga ccgcgtgcga ggagctggtt
       61 gagttatgaa cgcaattgga gtagttgagc tcatcagcgt tgcgcgtggg tacgccgcgg
      121 ccgacgccat gctcaaggcg gcaaatgtgc agcttcacgc ggccaaaccc gtatgcccgg
      181 gcaagtacgc catactcgtg tacggaggcg tggccgaggt gcagagttcg gtggaggccg
      241 gagtcgaggc gggaggccag ttcgtcgttg accagctggt aatcgccaac atacatcaat
      301 cggtatggcc tgcagtgatg ggagtcctgc cgcctcctgc aatccgagca ctcggcatta
      361 ttgagacata ctccatcgcc tcaaccgtgc ttgcggcaga tgctgcggtg aaggccgcca
      421 gtgtcgcgct ggtcgaggtg aggttggccc ttggtatggg cggtaaggct ttcacaaccg
      481 tgacgggtga ggtgagcgat gttgcggcgg ctgtagaggc cgcgaccatg ttggcgggcg
      541 accggggcat gctcaccgag gcagtggtga tcccggctcc tgccgcgggc cttacggagt
      601 ttaccatatg atggctggag gtgtggacat gcctgcttct ctcctgatta ccgcagcaga
      661 gcttgccggc aggaaggcgg gcgaccgcat aaccgttccg aaaggggcga tagtcactcc
      721 ggcggcgagg gattacgcta gtgaacacgg gatatgtatg gtctcggaag acgggttcgt
      781 catcgctcaa ggccggcccg gcacgcatga agatgatgcg agccggatcg taaggggcat
      841 tctcggcgag ctccgacgtg agctcgggcg cgtgcctacg agggatgaag ccgttgggtt
      901 cgtgcgagcc gtgatcgaac gggctcgcca ggaagcgact gatgtaattg acagttaaaa
      961 ggagaatgca tcatggcaac catgggcgcc attgaggcta tcaagggtcg gcgttctgtg
     1021 cgacggtacc ttcctaaccc tgtgcccagg gagataatcg aggacatagt ggactgcggg
     1081 cgattggcgc cttcggcccg caacgatcag ccctgggagt ttgtggtcgt gaccgaccag
     1141 acgatcaggg aggagattgc tgccgctgct acatacggca agttcattgc ggacgcgcct
     1201 gtgtgcgtag tagcatttgc caggcagacg cgcaggcata cggaggatac ctgtgcggcg
     1261 gtggagaata tgcttgttgc cgcttatgct cacggtcttg gaagttgctg gatagcaggg
     1321 gagaatactc cgtatgagaa ggagatcgcc aggatatgca aagcaccgga cgggcttcgg
     1381 ctggttgcgt tgttcccgct gggatacgcg aatgagaagc ccaggccgaa caagcgtgac
     1441 ttggaagagg tactccactg ggaggggttt taggcagcgc gtatcgcgtc tcgcggcgtt
     1501 tgccgcgctg atcttggcgg ccgcgaccgg gatcgctgcc gccgttgttc tttttgagat
     1561 gcgcatgcct gcccatctgg cgtcggccct ctccggcgcg gaaccgggcg atgccgcagt
     1621 ggccgatctg tgcgtatatg tacagagatg ctcgcacctg ggagcgccgg accttccggc
     1681 gcagcgtgtg cagtcgctgc tggaacggag aaccgcgcgt gatcgtgacg gggcgaccct
     1741 ggtgacgagt ccttcgcgtg ccagtctggc acgggaagcg catcagttag tgctttccga
     1801 gaacggcggg gaatgggagt ctggtttcca ccggagtaga ctggagagaa ttgcgcgtgt
     1861 gctggccatg gctgcaggcg acaggcctga gtcttacacc ctgactcttc tcgacaccac
     1921 acagaccaat gcggtctcca ctgcggatgg acgcatctac ataacacgcg gcctggctac
     1981 tacatcgaca gatgatgaaa tcgccatggt gctagctcat gaaatgcacc atatacgctc
     2041 aggccattgg tacagctggt gggcgcctga cgcccgtgat gtgggcaacg ggggcaacag
     2101 cggcaatgga agtggagagg gcgcaccgcc cgacccggtc tctgaagctg cccaagcgtt
     2161 ggcggaccta ggcgtggacg cgttgtcggg tgcggctacc acgtcgtacg cacaggagtt
     2221 cgaggcggat gcgcgcgggg tgttgctggc atctatgtgc gggtacgacg gtcgccagat
     2281 gtacaacgtg ctcacacgtc ttccgggcat gccggttact agccacccca gcgtcgccca
     2341 taggatcgca ggcgtcgcaa aggttctgga cgccatggaa gatcccgagt ggatatcatc
     2401 tcacaatccg gtggaggtag ctgtcagatc agttgaggct gctatgcggc gagaggtcgc
     2461 gtaccgccct ggggtatccg gagaggcgcc cggcctgccg gatgtggcgc gcgcgtgcca
     2521 ggcgacgctg gactggctga atgccactga ggcagagatg ataggcttca gatggctggg
     2581 agctgggagg gcggtctccg gtcgtcgtcg agtgctgggg cggaccatca tgatcgcgtc
     2641 tagcctcagc atcgttgata tcgcggttga taccgggttt gtgcgtgcaa ccgcaggaaa
     2701 accaggtgtc gtgtgcggca ggacctggtt gatccggcgt cgtggggtgt gggagccggt
     2761 ggcggctcaa gtccttccga gaacgcgatt cgcggcgcct gaatggctga agttcaagga
     2821 cgagttgcga ggctctgttg cccaagggcc caaagatgtg gatcgcgatg ttctgaggac
     2881 tgcccagatg tggcgggaca cggctgtgag tgattttccc gggcatctga cggtgtatgc
     2941 tcggggcaga ctcgaatcat cggcggcggc agactcatcc agcaggcacg acaatgggca
     3001 tgggcagacg ttggaggcta tcggatgggc acggttttta gggggcaggc aggcagagct
     3061 gcgcgcgtgg agcgtgcgct gcgaagcgca tagtccgaca ttggccacag tggcgttttc
     3121 atcgggattg actgtggacg gcgtgcccat aagcagcaac agcaccaggc ttaccctggt
     3181 gctggaggat ggccaatgga aagtcgctca ggtcaatttg gactgaatgt gatgtagata
     3241 gtaacctgtt cggagttgag atgaaccggc ctgctgatcg tcgccgtgcc gatcgcttca
     3301 tccaacctgc ccaggacatg atctagcaca tcggacggga ccgtaagcac tatggcctgt
     3361 gcctcgccag cgctatgtcg tgcgctgtgg actgcgatat tgcctgcgat gtcgcctgcg
     3421 cccggtagct tgacagcgcc gcctgcttca gctgcgatca gctcgagagt gtcgggaatt
     3481 tcatcggtga gtggtgtgga gtagcccacg tacaccgcgt cagccgtatc gcccgcattg
     3541 ggcgcgaacc caagcgcccc gcttcctggg cccaaaagca cgctgggcga ctcctcagcg
     3601 atctgtggag ccggaccggg cttaagccag atgctgtagg tgaaggccat cagtatgaga
     3661 gccgcagcgg cgccgacggc cacaaggttg gtgtaaaacc tgggaagccc cagtatcgaa
     3721 agcttgtatg cttttcggcg gcgcgcgtcc gactcggcct ccaacaggaa tgactgcttg
     3781 agccgtgcgc gaaactccgg cgcgggcgtc gcgccgcttt tcagggctct tactatggcc
     3841 caatcctcgc ccgtctgcaa ttcctccatt gcatccattt gttcatcgga ccgcacttgc
     3901 tttcacctct tcctctgtgg acaccaggga tcgcaatgcc gcaatcgccc gattgagcct
     3961 ggacttgact gtgcccagct ccactccgag ctggtccgca atctgctggt aactcagctc
     4021 catgaagtat ctcagctcga taacggatcg ttgccccggt ggaagcgcgt tcagcttgcc
     4081 gtagacatag cgcgcgaaat ccgcgtctgc cacgtggtct tcgggcccgg gcgcgtcatc
     4141 ctcaatctct ctgggcacgg agtcgccgcc atcgactcgc aaatccgcgt ccagcgacac
     4201 actggggcgc cttcgcctga tagaatccat aaccgcattg ttggccacgg tgtatatcca
     4261 tgtcgagaac tttgcttctc ctctaaatga atcgagcgac ttacaaagct tcaggaagac
     4321 cgctgacgtc acatcctcgg catcctgggc ggtgcgcagc ttggaatacg caagccgata
     4381 gaccttatcg aagtagcgtt catatatggc ctccattcct ctcgggtcgc ccgagaggtg
     4441 gagttggatt agctgttcgt ctgacgcgtc ggagtacata cggctcacct tcctggatgc
     4501 gcgtctggac ttcccgcggc gggcgggcgt cctatactac tacgccatcc acgcaggcgt
     4561 tcctgctcgg ggattcctac cacggcgtgg gcccgtgcat actccagcta cactcagtca
     4621 catgatatga cgcggcaggc ggtcggaggt tccgtgccgg agactggagg aattgccatg
     4681 gccccgaaga agaatctaag agtaatgatc aataaggcac tgggggtggt acagtggggc
     4741 tgagatttcg gaaatccatc aggatcggga agttcgccag gataaacata agcaagtcgg
     4801 gggtaggcgc gtcagttggt gttcccggct ttcgtgtcgg catgggggca gacgggaaag
     4861 tccgccggac gatgggaata cccgggaccg gtgtgtacca cactgaagtc ctcgcgtcgc
     4921 cgaagaacga ggcgacgaag cccaaacgga catgccaggc gtgcggcagg caggtggcgc
     4981 agaaggacag gttctgccgt cattgtgggg cccggcttgc cacctgaagc tagctggcgt
     5041 ttcctcatct cttcgtgtat cgactgtcac ggctcgtggt tgatgcgcgg cgacttcggt
     5101 caggccgtta tcacggcact cgggtgagtt gtgggtcctc ggggtccaag gcgtataagc
     5161 cgaacttctg ggtgtatccg tctcaggttc atacagtcct gcagcggttt atgaaggata
     5221 gtgcggagtg tgtctcgaag accatgaccg cgggaatccc ggaggatggg cggtgcgaat
     5281 gcggagagtt caagggaggt tgagctattg gcgggtgatg gtggtcggga gggcggcgcc
     5341 gggcggcgtc ttgttcccat agcctgggat gcgacgcggg gggcggtgcg cctgatagat
     5401 cagactcttc ttccgacaga gctaaaatac atcgatatcc gttcgttcga cgacatgtgt
     5461 gaggccatag cgatgctgcg tgtgcgaggg gcgccggcca taggggtggc tggggcgctt
     5521 gggatatatc tgggccttgt ggagagggcg ccggtatcgg ctgccggggt attcgaggcc
     5581 ctggatgagc tgtgcccgag aatgattggg gtgcgcccga ccgcggtcaa cctgtcgtgg
     5641 gcagtgaacc gggtcaggca ggccgtggag cgtgcggcag tcgctgcaga cgcggcaggc
     5701 gcggcagacg gagctcagcc gccacggtcg tacgggctgc tcgcgcagtg cggatcgctc
     5761 gaactgctcc gaccgcacga actgcttgcg gcggcgcttc acgaggcgat ggtgatagcg
     5821 gatgaagacg ccgcagcctg tcgccgtatt ggggagtatg gctcgcagct tataccggac
     5881 ggcgggcgcg tgctgactca ctgcaatgcc ggcgctctgg cgacggcggg gtgggggacc
     5941 gcgctggcag cgctctatgt cgcacaggag cagggcaaga ggttcagagt atgggccgac
     6001 gaaacccgac cgctgctcca aggctcccgc ctaaccgcct ttgagctggc tgaagcgggg
     6061 atcgacgtga ctgttatatg cgataacatg gcggcgtcat tgatgcgtgc ggggcaggtg
     6121 gatatcgtca tagtcggggc cgatcgagtt gcggctaacg gcgacgtgtg caacaagatc
     6181 gggacgtatg gtctggcttg ccttgccaac atgcaccgga taccgttctt tgtcgcctgc
     6241 cccacgtcta ctctggatct ttcgcttagg gcgggcgacg agatacccat agagcagagg
     6301 gatccgcggg aggtcactca cgggttcgga cggcagactg cgccggacgg agtgggggtc
     6361 tacaacccgg cattcgatgt caccccag
//
LOCUS       DAOTAC010000138         6354 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_10371_length_6354_cov_5.239562, whole genome shotgun sequence.
ACCESSION   DAOTAC010000138 DAOTAC010000000
VERSION     DAOTAC010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6354
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10371_length_6354_cov_5.239562"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="PLM74_06965"
     CDS             complement(<1..423)
                     /locus_tag="PLM74_06965"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HON42852.1"
                     /translation="MNIAEYSVRRPITVTIVVAACLLFGFLSLGRIGLDLLPQMNLPM
                     AVVVTVYPNADPETIDSLITVPVERSLKSARNIKSVTSTSMENVSVSLAEFNWGSDLG
                     SVQEEIRAGMDRIRYQLPEGVQDPIVSLIDPSQLPLMML"
     gene            complement(452..1198)
                     /locus_tag="PLM74_06970"
     CDS             complement(452..1198)
                     /locus_tag="PLM74_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42853.1"
                     /translation="MGCLEFDHHTGRRNAESAARILILVLLFTILMSTAVALCASAAP
                     TGDEIAARVTDKLKEIRSVQGDIVASFSDPSSKNPINMTIRVSADRSSSITRMEITQH
                     AVFEGQIVILDMKKDLTTVYMPVTGQAYRGKSATIAAQLGLDMGSFDLDNLLSMDPAG
                     MMSFKYLRQDKYDRLPHYVVQTKPNVDVEGHQLVWVDCETYMVRKIEAFDGQGKQVAS
                     ITINNLKINPKLDPVRLRELPKGTRITDVK"
     gene            complement(1279..2043)
                     /locus_tag="PLM74_06975"
     CDS             complement(1279..2043)
                     /locus_tag="PLM74_06975"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine biosynthesis TtcA family
                     protein"
                     /protein_id="HON42854.1"
                     /translation="MIKNYLSKPYHTKLWRAIYEYGLIEPGDRILIGLSGGKDSMFLT
                     YALSTLRSYSDIPFEIEAVTVDIGFPNPLNVDRIKEACAAMGVTHHIVPTKIAQVIEE
                     RRGKTNPCAVCSYFRRAAMNRFAFEHGFNKVALAHHEDDAIETFLMNILYAGKLCTLP
                     WKTTLTRTAVTVIRPMMYLSEKDVIKAVKRAGIEAVPSCCPHSSGTMRARVKRLVLEL
                     VKENKMVRANLVASMRNGNNVELWPPIGRHNRQNLC"
     gene            complement(2130..2220)
                     /locus_tag="PLM74_06980"
     tRNA            complement(2130..2220)
                     /locus_tag="PLM74_06980"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2184..2186),aa:Ser,seq:gct)
     gene            complement(2257..2345)
                     /locus_tag="PLM74_06985"
     tRNA            complement(2257..2345)
                     /locus_tag="PLM74_06985"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2309..2311),aa:Ser,seq:tga)
     gene            2569..3612
                     /locus_tag="PLM74_06990"
     CDS             2569..3612
                     /locus_tag="PLM74_06990"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HON42855.1"
                     /translation="MRYIPIEQVEPGMILARNVWGPNGQLLLTANTVLKESYVSRLRN
                     LQYSALYVKSYPDEPVENILEPVKQETIVQATQALKTVSEAAVTTGRLDVDRVLEVVS
                     DIVDQVLCNRNVVYSIADIRSFDDYTYGHSVDVCVLAVMCAAELGMGRYDLLDLGTGA
                     ILHDLGKLFTPKSILSKETPLSDEEWDVIRRHPWDGFQMLRKQVPLMPAHVAFQHHER
                     YDGSGYPRGLTDCQTLDIAKVTAVADSFNAMTSDRPYRKALMPHEALPILAREAGKSY
                     NPDVVRAFIGIVAAYPLGTIVKLSDGSVATVVGVTKELYDVEVLSGPREGNHISIHHE
                     RSESDIHIIERID"
     gene            complement(3640..4917)
                     /gene="serS"
                     /locus_tag="PLM74_06995"
     CDS             complement(3640..4917)
                     /gene="serS"
                     /locus_tag="PLM74_06995"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009609894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HON42856.1"
                     /translation="MLDLKFVRSNPDVVRDSLIRRGETTDGLDALLAADEKRREILVE
                     VEELKRRRNEASERVARLKREGADASEIIDEMKGVSERIRGLDEELAAVESAITELLL
                     GIPNIPHESVPTGRDDSENVEVRRWGQPRQFGFEPKAHWDLGTDLDILDFERASKITG
                     ARFAIYKGTGSRLVRALVNFMLDLHTQEHGYTEVFPPFLVNGASMVGTGQLPKFADDM
                     FKCEGLDYYMVPTAEVPVTNMYRGEILDGACLPVKHVAYTACFRAEAGAAGRDTRGLI
                     RQHQFDKVELVKFVRPETSYDELESLVSDAQSVLEILELPYRVTMMCTGDVGFSAAKK
                     YDLEVWMPSYNRYVEISSCSNFEAFQARRADIKFRPAPGAKAELVHTLNGSGLAVGRT
                     IAALLENWQQADGSVVIPERLRPFMGGLEAITK"
     gene            complement(6059..>6354)
                     /locus_tag="PLM74_07000"
     CDS             complement(6059..>6354)
                     /locus_tag="PLM74_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015907413.1"
                     /note="catalyzes the formation of acetyl-CoA from pyruvate
                     and coenzyme A; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HON42857.1"
                     /translation="MAVIQPCVLGWKYPSDQTIELARLAVDTCFWPLYEVVNGEYKLT
                     YKPKEKKPLADFLRKQDRFRHLFKPENEHILAELQEEVDRKWALLLTKCGEQA"
BASE COUNT         1322 a         1910 c         1755 g         1367 t
ORIGIN      
        1 gagcatcatc agcggaagct gggacgggtc gatgaggctg acgatggggt cctgaacacc
       61 ttccgggagc tggtacctga ttctgtccat gccagcccga atctcttcct gcaccgaacc
      121 gagatccgac ccccagttga actcggccag gctaactgag acattctcca tgctggtgga
      181 agtgacagac ttgatgtttc gggccgactt gagcgaacgc tccacgggca cggtgatcaa
      241 gctgtcgatt gtttcggggt cagcattggg gtacactgtg acgactaccg ccattggtaa
      301 gttcatctgt ggcagaaggt ctagcccgat tctgccgagc gacaggaaac cgaagagaag
      361 gcatgccgca acaactatgg tcaccgttat cggcctgcgc acggagtatt cagctatgtt
      421 caaccaggtt cccccctaag gttgcggacc ttcacttcac gtcggtgatc cgggtgccct
      481 ttggaagctc ccggagtctg accggatcga gtttcggatt gatcttgaga ttgttgatgg
      541 ttatggaggc tacctgctta ccctggccat caaaggcttc gatcttgcgg accatgtaag
      601 tctcgcaatc cacccagacc agctggtgcc cttcaacatc cacgttgggc ttggtctgta
      661 ctacatagtg cggaagtcgg tcatacttgt cctgccgaag gtatttaaaa ctcatcatcc
      721 cggcagggtc catggaaagc aagttgtcga gatcaaatga ccccatgtcc aggcccagct
      781 gggctgcgat cgtcgccgat ttgcccctgt aggcctgacc ggtaacgggc atgtacacag
      841 tagtgagatc cttcttcata tcgagtatga ctatctgacc ttcgaatact gcgtgctgag
      901 taatctccat ccgcgtgatg ctgctgctcc tgtcggcact gaccctgatc gtcatgttga
      961 tcggattctt gctagacgga tcagaaaacg acgcaacaat gtctccttga accgatcgga
     1021 tctccttgag cttatccgtg actcttgcag cgatttcgtc gcctgtggga gcagccgacg
     1081 cgcagagggc aacggcggtg gacatgagta tagtgaatag taatacaagt atgagtattc
     1141 ttgcggccga ttctgcgttt cgccgtccgg tatggtgatc aaactcgaga catcccacag
     1201 gtagttcccc caatcactgc aagtacagca caatagacca cacgccatag ctattatagg
     1261 catcgaggaa aacgcctgtc aacaaaggtt ctgcctgtta tgacgcccga tgggcggcca
     1321 tagttccaca ttgttcccgt ttcgcattga cgcaacgaga tttgctcgaa ccatcttgtt
     1381 ctctttcaca agttcaagta cgagtcgctt gactctggcc cgcatcgtgc cagacgagtg
     1441 cgggcagcag ctaggaacgg cctctatacc tgccctcttg actgccttga tgacatcctt
     1501 ctcagacagg tacatcatgg ggcggatcac cgtgacagcc gttcgcgtca gagtggtctt
     1561 ccatggcaaa gtgcagagct tgccggcgta cagaatgttc atgaggaatg tctctatggc
     1621 atcgtcctca tgatgggcca gggcaacctt gttgaaccca tgttcgaatg caaaccggtt
     1681 catggcagcc ctgcggaaat aggagcagac cgcgcacggg ttggtcttgc cgcggcgctc
     1741 ctcaattacc tgcgcgatct tggtaggtac gatatgatgc gtgactccca tggctgcaca
     1801 tgcctctttg atccggtcga cgttgagcgg gttcgggaaa cctatgtcca cagtgaccgc
     1861 ttcgatctca aatgggatgt ctgagtatga tctgagcgtg ctcagcgcgt atgtcaagaa
     1921 catgctgtcc ttcccgcccg ataggccaat gaggatccgg tcgcccggct caatgagtcc
     1981 atactcgtag atggctctcc acagtttggt gtgataaggc ttggataggt agttcttgat
     2041 caccggctga ccacctacca aatgaaaagt ggcatgaact ccggcttccg ccgggccatg
     2101 ccgcaagaca tggaacatgt atctgaatct ggcggagaga gggggattcg aacccccgct
     2161 agggtcaccc ctactaatgg tttagcaaac catccccttc agccgcttgg gtatctctcc
     2221 gctaaggctg aaacctaatt gtgttgctct cgattctggc ggagggggtg ggattcgaac
     2281 ccacggccca ttgctgagac accggttttc aagaccggct ccataaacca ctcggacacc
     2341 cctccggccc gatgctaaac agagtataca ccgcactgta acgtgcgtca aggcaagaag
     2401 tcgcgctgtc gcgcttgttg ggcgttgcac agcgggtccc ggctgttcag caatgtaccg
     2461 atgatagcag gatattgacg ataaatgtgg aacgagcttt tgtggaccga ccaggcaagt
     2521 taggcccagc cgacataatc gcaacacctg aacctacgag ggatggacat gcgctacata
     2581 cctattgagc aagtagaacc tggaatgata ctggcccgca atgtctgggg ccccaacggg
     2641 cagttgctac tgacggcaaa taccgtgctt aaagagtctt atgtctcgcg cctgcgcaac
     2701 cttcagtact cggccctgta cgtgaagagc taccctgacg aaccggtaga gaacattctg
     2761 gagcctgtga agcaggaaac catagtccag gcaactcagg ccctaaagac agtctcggag
     2821 gccgcggtca cgacaggtcg tctggacgtc gaccgtgtcc tggaagtggt aagtgacatc
     2881 gtcgatcagg tgctgtgcaa ccgaaatgtg gtctacagca tcgccgacat aaggtcgttc
     2941 gacgactata cgtacgggca ctctgtggat gtgtgcgttc tagcagtcat gtgcgccgca
     3001 gagctaggaa tgggcaggta cgacctgctc gacctgggta caggcgcaat tctccatgat
     3061 ctcggcaagc tcttcacgcc caagtcaatt ctctctaagg agacgccgct gtcagacgaa
     3121 gagtgggacg tcatcaggcg ccatccgtgg gacggttttc agatgctccg caaacaggtg
     3181 ccgctgatgc cggcccatgt cgcttttcag catcatgaga ggtatgacgg gtccggctac
     3241 cctcgcgggc tgaccgactg ccagaccctg gacatcgcca aggttacggc cgtggccgat
     3301 tccttcaatg ccatgacctc tgacaggcct tacaggaaag ccctcatgcc gcatgaggcc
     3361 ctccctattc tagctcgaga agcgggtaag agctacaacc ccgatgtcgt gcgcgccttc
     3421 atcggaatcg tagcagctta cccactaggc actatcgtga agcttagtga cggatccgtg
     3481 gctacagtcg taggagtcac caaagagcta tacgacgtag aagtattgtc gggcccgcgc
     3541 gaaggtaatc acatatcgat acatcatgag cgtagtgagt ccgacatcca tatcatcgaa
     3601 cgcatcgact agaccgtctt gatcgtctgc cgggccgcgc tacttcgtga tggcttcaag
     3661 gcctcccatg aacggtcgca ggcgctccgg tatcacgaca gagccatccg cctgttgcca
     3721 gttctccagg agggcagcga tcgtccgccc cacggccagc ccggacccgt tcagagtgtg
     3781 gacgagctcc gccttcgcgc ctggagccgg tctgaacttg atgtcggcgc ggcgagcctg
     3841 gaatgcctcg aagttgctgc atgacgagat ctccacatac cggttgtagc tcggcatcca
     3901 cacttccaga tcgtatttct tggcagctga gaatccgacg tctcccgtgc acatcatagt
     3961 gacacggtaa ggcagttcca ggatctccag caccgactgc gcatcggata ccaggctctc
     4021 caactcgtcg taggaagtct ccgggcgcac gaacttgacg agctccacct tgtcgaactg
     4081 atgctgccgg ataaggcccc tcgtgtcgcg cccagccgcg cccgcttctg ctcggaagca
     4141 ggcggtatat gcaacgtgtt tcaccggcag gcaggcgcca tcaagtattt cgccacggta
     4201 catgttggtc accggcacct ctgccgtagg caccatgtag tagtcgagtc cttcgcattt
     4261 gaacatgtca tcggcaaact tgggcagctg gccggtccca accatggaag ccccgttcac
     4321 caggaacgga gggaacactt ccgtgtagcc gtgctcctgg gtatgcaggt cgagcatgaa
     4381 gttgaccagc gccctgacga ggcgggagcc cgtccccttg tagatcgcga accgtgcgcc
     4441 cgtgatcttg gaagcgcgct cgaaatccag gatatcgagg tcagtgccga gatcccagtg
     4501 ggccttgggc tcgaaaccga actgcctcgg ctgcccccat cggcggactt ccacattctc
     4561 gctgtcatcg cggcctgtgg gtaccgactc gtgcggtatg ttgggtatgc caaggagcag
     4621 ttcagtgatt gctgattcca ctgcagcaag ttcctcgtca agcccgcgaa tgcgctcaga
     4681 gacgcccttc atctcgtcta tgatctcgga cgcgtcagcg ccctcgcgct tcagacgcgc
     4741 aaccctctcg gaagcctcat tacgccggcg cttcaactcc tccacttcga caaggatctc
     4801 ccggcgcttc tcatctgcgg caagcagggc gtccagccca tcggtggttt cgcctctgcg
     4861 gatcagcgag tccctcacca catccggatt tgagcgaacg aacttcagat caagcatgtc
     4921 aaacacccct cacactgaat ggtcactact gaatggtcac tagtgaatgg ttactctggt
     4981 agttccggaa tcaaaaaacc cgccccaacc ggcgatcatc gccgggggcg ggtgaaactt
     5041 cccgcgttgc caccccgatt ggccgcctgg acaagcatcc gaggttcctt accaccacgc
     5101 gcttgttctc aggcgtaccc actcgttgcc gctatcacgg gcggacccga cacggcttac
     5161 tcttgttcgg ccggccgctc cagggcggat tcacttgcct tcacccaccg gttcacaccc
     5221 gcatcctccg acgcccgccg cctgttcggc gcttccgttc aagcggtcgg agggcgcatc
     5281 cggctctctg aggagagaag cacaggctac tgatcccctt catcgcgttt tcagtgtgtc
     5341 aggactgcgg cttgcgaatt ccccgatgca gccccttacc tttgatctat gatatcaccc
     5401 agtcctggaa agtcaatacc ccttcgcggt ccccgtagcg gtccccgtag cggtccccgt
     5461 agcggtccga tttcgcagtc gatccgcgca ggcgtgtggg aatccgtcgg cgatcggatc
     5521 ctgtgcccta cggaacacct ttttcgacca tcctgtgtcg gttgacacag gcctcccgaa
     5581 aatcctggcc cacagggcac aggttcccgg aacccatcgg cagtcgtcgc gcgaggcgaa
     5641 acccccggat gcgaaaaccc cagctagggc ccgcgggccg aaacggtcgg catcaaccat
     5701 ggcgggcggg cgaccgtcgg cgaccgtcgg cgatcgcatc ctgtgcccta cgggacacct
     5761 ttttcgacca tcctgtgtcg gttgacacag gcctcccgaa aatcctggcc cacagggcac
     5821 aggttcccgg aacccatcgg cagtcgtcgc gcgaggcgaa acccccggat gcgaaaaccc
     5881 cagctagggc ccgcgggccg aaacggtcgg catcaaccat ggcgggcggg cgaccgtcgg
     5941 cgaccgtcgg cgatcgcatc ctgtatatat gaaggagggc ggctaggccg ccctccaacc
     6001 tgatattgtc gtttcgatat tgccgtctat acttccgcgc tgcgatccga ccggctcgct
     6061 acgcctgctc cccgcacttg gtaagaagga gggcccactt gcggtccact tcctcctgta
     6121 gctcagccag gatatgctcg ttctcgggct tgaacaggtg gcggaaccta tcctgcttgc
     6181 ggaggaagtc tgcaagaggc ttcttctcct tgggcttgta ggtcagcttg tactcgccgt
     6241 tcacgacctc atatagcggc cagaagcagg tgtccacagc caaccgcgcc agctcaatgg
     6301 tctgatccga cgggtacttc caccctagca cgcacggctg gatgacggcc atga
//
LOCUS       DAOTAC010000139         6351 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_10376_length_6351_cov_5.412325, whole genome shotgun sequence.
ACCESSION   DAOTAC010000139 DAOTAC010000000
VERSION     DAOTAC010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6351
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10376_length_6351_cov_5.412325"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1719)
                     /locus_tag="PLM74_07005"
     CDS             complement(<1..1719)
                     /locus_tag="PLM74_07005"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HON42858.1"
                     /translation="MDFGSMAFLAPVSALVALGVAAYLSVSISRKDEGTQQMRSISEA
                     IREGASAYLKRQYRGVVLFFAVMFVLLLILAGTGHLSYFTPFAFLSGGFFSGLAGYIG
                     MSTATKSNARTAQAASRSLNEGLRLAFSSGSVMGLMVVGLSLVHLSIWYFGLTWYYTS
                     IQPITDSALRLAQITSTLLTSGMGASSMALFARVGGGIFTKAADVGADLVGKVEAGIP
                     EDDPRNPAVIADNVGDNVGDVAGMGADLYESYVGSIIATSALGASMSLGVNWVVLPLV
                     LAAVGVLASVVGSFMVKTGERAEQSLLLAALRRGVNVSAVLVIAVSWFLVRNLLGPEY
                     TGIFWSIIAGLASGVIIGRITEYYTSADYKPTQIVAHSSLTGPATVIISGMSTGMMST
                     AMPILVVGAAVIVSYYLSGGASNQIAGLYGIAISAVGMLSTLGITLATDAYGPVADNA
                     GGVAEMAGLPKNVRERTDALDSLGNTTAATGKGFAIGSAALTALALIAAYRDQIVLIA
                     PGRDFLFSLMTPSVLVGVFVGGMLPFVFSALTMQAVGRAAEGIVNEVRRQFREIPGLM
                     EGKARPD"
     gene            complement(1849..2400)
                     /locus_tag="PLM74_07010"
     CDS             complement(1849..2400)
                     /locus_tag="PLM74_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005951625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HON42859.1"
                     /translation="MAKRITTPLSDETIAGLKAGDQVLLTGVVYTARDAAHKRLIALI
                     KEGKELPIDLQGQVIYYVGPAPAKPGDPIGSAGPTTSMRMDPYAPTLMEHGMKGMIGK
                     GMRSKEVRDAMVRHRAVYFAAVGGAAALVAKRIKSAEVVAYEDLGPEAIRKLYVEDLP
                     LFVIDDIHGDDLYEQGVAKYRKA"
     gene            complement(2422..3282)
                     /locus_tag="PLM74_07015"
     CDS             complement(2422..3282)
                     /locus_tag="PLM74_07015"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HON42860.1"
                     /translation="MGCAKPRVIQAGDIADAMEKLCISACCNLPEENLERIEAYAATE
                     ESEVGRQVLEMILENHRLAAREQRPACQDTGLAVVFLELGQDVRIEGGDLVAAINEGV
                     RRGYEKGYLRKSVVAEPLFERKNTRDNTPAIIHTSIVAGDKLKITLAPKGGGAENMSG
                     VAMMIPAAGVEGVRKFVIEQVRKAGPNPCPPVVVGVGIGGNFEVAPLLAKKALVRPMG
                     SRNPNPAYAALEDELLEAINKLGIGPQGLGGSTTALAVHVEYAPCHIASLPVAVNLNC
                     HVSPHQSITL"
     gene            complement(3379..4623)
                     /locus_tag="PLM74_07020"
     CDS             complement(3379..4623)
                     /locus_tag="PLM74_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019720733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HON42861.1"
                     /translation="MSLREDALKLHKDSMGKIEVVSKVPLRNKQDLSLAYTPGVAEPC
                     KEINADYDKIYDYTNKGNMVAVVTNGTAVLGLGDIGAGAGMPVMEGKAVLFKGFAAVD
                     AFPICLDTKDPDEIVMIVKRLEPTFGGINLEDISAPTCFEVEDKLKKISNIPIFHDDQ
                     HGTAIVTCAAMISALKLVGKKASEVKVVVNGAGAAGIAVTKLLMTVGVKDAILCDSKG
                     AIYEGRPFGMNPYKDEIAALTNRSKLQGKLADVIRGADVFIGLSVAGCVTREMVASMA
                     KDAAIFAMANPVPEIMPDEAKAGGAKVIATGRSDFPNQINNVLAFPGLFRGALDARAS
                     AINEEMKLAAVYAISDIVTDSELSPDYIIPGPFDTRVAPAVAAAVAKAAMETGVARVK
                     VDPEEIAARTIKMVEAVRAAWK"
     gene            complement(4637..6109)
                     /locus_tag="PLM74_07025"
     CDS             complement(4637..6109)
                     /locus_tag="PLM74_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018594393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HON42862.1"
                     /translation="MGCAKKCRIRVEKDFIGERDVPEQALYGIQSLRGSENFHITGQK
                     MHRGLIVGLAMVKKAAALANAELGYLPPDVGSAIAAAADEIIAGKHIDEFIVDSIQGG
                     AGTSMNMNANEVLANRASEILGGGKGADSLVSPLNHVNLGQSTNDALPTAARIALLSE
                     LGPTVEALRQLAAAMRDKGDEFKDVLKMGRTHLQDAVPITLGQEFHAWAKAFDRDADR
                     IQYSAEGLKSVNLGGTAVGTGLNADVEYIRLAVEMLSKISGYNLTSPKDLVDATQNLD
                     SLAFVSACLKVCAINLIKIANDLRLLASGPMAGLYEIQLPAVQPGSSIMPAKVNPVIA
                     EVTNQVGFHILGNDLAVTMAAQAGQLELNVFQPVLLYNMLQSIEILGNVARTLKDRCI
                     VGIKANKERCAKMVEENVGVVTALVPYIGYQSSCDIAKEARATGRSVRALVIEKGLMT
                     ENELNHVFAPSRVTRPGKPDAELNRHRVAVADNESAATKA"
BASE COUNT         1251 a         2001 c         1723 g         1376 t
ORIGIN      
        1 atccggcctg gccttgcctt ccatcaggcc cgggatctca cggaactgcc tgcgcacctc
       61 gtttacgatt ccctcagctg cgcgccccac ggcctgcatc gtcagagcag agaacacaaa
      121 cggcagcatt ccgccgacga acacgcccac aagcacggac ggggtcatca ggctgaacaa
      181 gaaatcgcgc cccggagcga tcaagactat ctggtcgcgg taggcggcaa tcaacgccag
      241 cgcagtcaaa gcagcagaac cgatcgcaaa gcccttgccg gtggctgcgg tcgtgttgcc
      301 cagggaatcg agcgcatctg tgcgctcgcg gacgttcttg ggcagacccg ccatctccgc
      361 cacgccgcct gcattgtcgg caactggacc gtacgcgtcg gttgccagcg taatgccgag
      421 agtagatagc atgccaacag ctgagatcgc tatgccgtaa agaccggcga tctggttgga
      481 agcgccgcca gacagataat agctgactat gaccgccgcg ccgaccacca aaataggcat
      541 cgcagtcgac atcatgcccg tcgacatgcc cgatattatc actgtggcgg ggccggtaag
      601 cgaagagtgc gctacgatct gggtgggttt gtagtcggcc gatgtgtagt actcggttat
      661 gcgcccaatt atgacacccg acgccagtcc ggctatgatg gaccagaaga tgccggtgta
      721 ctcaggaccg agcaagttgc ggaccagaaa ccagctgact gcaatgacta gcactgcgct
      781 cacattcacg ccacgacgga gagccgccag gagcaatgat tgctccgcgc gttcaccggt
      841 tttcaccatg aaactgccga ccacggatgc caggacgccc actgcggcca gaaccagcgg
      901 cagcactacc cagttcacgc ccaggctcat tgaagcgccc agagcggagg tggcaatgat
      961 ggagccaaca tacgactcgt acaggtccgc gcccatgcct gcaacgtcgc ccacgttatc
     1021 gcctacgttg tcggcgataa cggcagggtt cctaggatca tcctcaggta tacccgcttc
     1081 cactttgccc acaaggtcag cgcccacatc ggcggctttg gtgaatatgc ctcctcccac
     1141 acgggcaaag agcgccatgg aactcgcgcc catgcccgaa gtgagcagtg tggacgtgat
     1201 ctgcgccagg cgaagcgcag aatccgtgat cggctgaatc gatgtgtagt accaagtgag
     1261 cccaaagtac caaatggaca ggtgaaccag ggaaaggccg acgaccataa ggcccatcac
     1321 tgagccagac gagaatgcca gccgaagacc ttcgttcagg cttcgcgacg ccgcctgcgc
     1381 cgttcgcgca ttcgatttgg tcgccgtaga catcccgatg tatccagcca gcccagagaa
     1441 aaaccctccg ctcaggaaag cgaaaggcgt gaagtagctc aggtggccgg tgccggccag
     1501 gatcaagagc agcacgaaca tgactgcgaa gaacaacaca acgccgcgat actgtcgctt
     1561 caggtaggcc gaagctcctt cccttatggc ctcggagata cttcgcattt gctgagtgcc
     1621 ttcgtcttta cgacttatcg acacagacag ataggctgcc actccaagcg cgaccaaagc
     1681 gctgacgggc gccaagaatg ccatagagcc gaagtccatt ccagctacgc ccgaaaagtg
     1741 attcccgggc gttttccctc cctccattcg atgcagcagg ccaacataac cagaatatca
     1801 caggtaggcg ccgcggtcaa agagaacccc gccttgcgct ccgcgacact acgccttccg
     1861 gtatttcgca actccctgct cgtacaaatc gtcgccgtgg atgtcatcga tgacgaacag
     1921 tggaaggtcc tctacataca acttgcgaat ggcttcgggc ccgaggtcct cgtaggccac
     1981 aacttcagcg gacttgatcc tcttagcaac gagcgccgcc gctccgccca ctgccgcgaa
     2041 gtagaccgcg cgatgacgga ccatggcgtc cctgacctct ttggatcgca tacctttgcc
     2101 gatcatgccc ttcattccgt gctccatcag cgtaggcgcg tagggatcca tgcgcatgct
     2161 ggtagtaggg cccgccgagc ctatcggatc tccgggtttg gcaggcgccg gtccgacata
     2221 atagatcacc tgcccctgca gatcgatagg aagttccttt ccttccttga taagagcaat
     2281 cagtctcttg tgagctgcgt ctctggctgt atagaccacg cctgtcagca gtacctgatc
     2341 ccctgccttc aggcccgcaa tagtctcatc actcagcggg gtagtaatgc gttttgccat
     2401 ttgacttcct cccttccctg cctacagagt gatgctctga tgaggcgata catggcagtt
     2461 caagttgacg gccaccggca agctggcaat gtggcacggc gcgtattcaa cgtgtacggc
     2521 gagcgcagtc gtactgccgc ccaggccctg cggcccaatg cccagcttgt taattgcctc
     2581 gagaagctcg tcctccaagg ctgcatatgc cggattcgga tttctgcttc ccatcgggcg
     2641 caccagcgct ttcttggcga gcagaggagc gacctcgaag ttgcccccta ttcctacgcc
     2701 cactaccacc ggcgggcagg gattgggtcc ggcctttcga acctgctcga tcacgaactt
     2761 ccgcacgccc tcgacgcccg ctgcagggat catcatggca acaccgctca tgttctcagc
     2821 tccaccgccc ttgggcgcca aagttatctt gagcttgtcg cctgcaacaa tgctggtatg
     2881 gatgatggcc ggcgtgttgt cgcgagtgtt cttgcgctca aacaacggct cggccaccac
     2941 agacttacgc aggtagcctt tttcataacc gcgccgcacg ccttcattga tagccgcaac
     3001 aaggtcgccc ccctcgatgc ggacatcctg cccgagctcg aggaagacca cggcgagccc
     3061 ggtatcttga cacgcgggcc tctgctccct ggcagcaaga cggtggtttt ccagtatcat
     3121 ctcgagcact tgccgcccca cctcagactc ctcagtggcg gcatacgctt ctatcctctc
     3181 gaggttctct tccggcagat tgcagcaggc gctaatgcac agcttctcca ttgcgtctgc
     3241 gatatcgccg gcctgaatga ctctaggctt agcgcacccc atcgtgtcac cctccctgtc
     3301 atctggatcc acttgttcag gacatggtca accgcagcgc gacccggatc cgggccgtgc
     3361 tgcggtcgat tgcccaccct acttccacgc agccctaact gcttctacca tcttgattgt
     3421 gcgggccgct atctcctcgg ggtcgacctt gaccctggct acgccggtct ccatggccgc
     3481 cttggcgact gcagctgcca ctgccggagc cactcgtgtg tcgaatgggc cggggatgat
     3541 gtaatccggg ctcagctcgc tgtcggttac gatgtcgctg atggcataga ctgccgccag
     3601 tttcatctct tcgtttatgg cgctcgccct agcatctagt gcaccgcgga acagtccagg
     3661 gaaggcgagg acgttgttta tctggttggg gaaatcggat cttcccgtcg cgatgacctt
     3721 ggctccgccg gccttagcct catcgggcat gatctccggc acagggttgg ccatggcgaa
     3781 gatcgcagca tccttggcca tgctggccac catctcgcga gtcacacagc ccgctaccga
     3841 aaggccgatg aacacatcgg cgcctcggat gacgtccgcc agcttaccct ggagtttgct
     3901 cctgttggtg agcgcggcga tctcgtcctt gtaggggttc atgccgaacg gcctgccctc
     3961 atagatggcg cccttggagt cgcacagaat cgcgtccttg acgcccaccg tcatcagcag
     4021 cttggtgacg gcaatgcctg ccgctcctgc cccgttcacg actaccttga cttccgatgc
     4081 ctttttccct acgagcttca aggcgctgat cattgcggca cacgtcacga tggccgtgcc
     4141 gtgctggtcg tcgtggaaga ttgggatgtt gctgatcttc ttgagcttgt cctcaacctc
     4201 gaaacacgtt ggcgccgaga tatcctccag gtttatgccg ccgaacgtcg gttcgaggcg
     4261 cttgactatc atgactatct cgtcgggatc tttggtgtcc aggcagatag ggaacgcgtc
     4321 cactgctgcg aatcccttaa acagaaccgc cttcccttcc atgacaggca ttccggctcc
     4381 tgcgccgata tcgcccaggc caagtacagc cgttccgttg gtgaccacag ccaccatatt
     4441 ccccttgttg gtgtagtcgt agatcttgtc gtagtcggca ttgatctcct tgcacggctc
     4501 agcgacgcca ggcgtatacg caaggctcag gtcctgctta tttcggaggg ggaccttgct
     4561 gactacttcg attttgccca tgctgtcctt atggagtttc agggcgtctt cccttagtga
     4621 catgcgtgct cctcctttaa gctttcgtgg cagcgctctc gttgtcggca acagctactc
     4681 tgtgcctgtt tagttctgca tcaggcttgc ccggcctggt cactctggac ggggcgaaca
     4741 cgtgattcag ttcgttctcg gtcatcaatc ccttctcgat aacgagggcg cgaacactcc
     4801 tgccggttgc ccgagcctct ttggcgatat cgcacgagga ctgatagccg atgtatggaa
     4861 ctagggccgt gacgacgccc acgttctctt cgaccatctt ggcgcaacgc tccttgttgg
     4921 ctttgatgcc tacgatgcat ctgtctttca gcgttcgggc aacattgccc agtatctcga
     4981 tggactgaag catgttgtag agcaggactg gctggaacac gttgagttcg agctgcccgg
     5041 cctgcgctgc catggtgacc gcaaggtcat tcccgaggat gtggaacccg acctggttgg
     5101 tgacctcggc aatgaccggg ttcacctttg ccggcatgat tgaagatccg ggctgaaccg
     5161 cgggcaactg tatttcgtat agcccggcca tggggcctga tgcgagcaac ctgaggtcat
     5221 tggcgatctt gatcaggttg attgcgcaca cctttaggca tgcagacacg aatgccaggg
     5281 aatcgaggtt ctgtgtggca tcgaccaggt ccttgggcga cgtcaggttg tacccgctta
     5341 ttttgctcag catctcaacc gccaaacgga tgtactccac atcggcattg agccccgtac
     5401 ctacggctgt accgcccagg ttgacgctct tcagcccttc agctgagtac tgaatccggt
     5461 ccgcgtcgcg atcgaacgct ttggcccatg cgtggaactc ctgcccgagg gttatgggaa
     5521 cagcatcctg tagatgggtt cggcccatct taagtacatc cttgaactcg tcgcccttat
     5581 cacgcatagc cgcagccagc tggcgcaggg cctccaccgt cggcccgagt tcactgagga
     5641 gcgcgattct tgcagcagtg ggaagcgcat cgttagtcga ctgcccgagg ttcacatgat
     5701 tgagcgggct tactagactg tcggcgccct tcccgccgcc gagaatctcg ctagcccggt
     5761 tggctaggac ctcattggca ttcatgttca tggaggtgcc ggcgccgccc tgaatcgagt
     5821 ctacaatgaa ctcgtcgatg tgcttgccgg caatgatctc atctgccgca gccgcaatgg
     5881 ccgacccgac gtccggcgga aggtagccta gctctgcgtt ggcaagcgcc gcggccttct
     5941 tcaccatggc cagacccacg atcagcccac gatgcatttt ctgccccgtg atgtggaagt
     6001 tctcgctacc acgaagcgac tgaatgccgt acaaggcctg ctccggcacg tccctctctc
     6061 cgatgaagtc tttctccact cttatcctgc attttttcgc acagcccatt tcgcttgcac
     6121 cgctccctgt atgatgtttt cggaatggag catccttggc ggaaccgttg cgcctgatga
     6181 tacattcctt atggaacttc gtcagtaatg atgaattctc cttcgttgca catactggaa
     6241 caagaatgat gaaatcccac gaacccccca gctaatgtca ggggggttcg caaatcaaaa
     6301 atccggcggc gacctactct cccgggcggt ctcccgccaa gtaccatcgg c
//
LOCUS       DAOTAC010000140         6317 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_10438_length_6317_cov_5.682050, whole genome shotgun sequence.
ACCESSION   DAOTAC010000140 DAOTAC010000000
VERSION     DAOTAC010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6317
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10438_length_6317_cov_5.682050"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1150)
                     /gene="porA"
                     /locus_tag="PLM74_07030"
     CDS             complement(<1..1150)
                     /gene="porA"
                     /locus_tag="PLM74_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HON42863.1"
                     /translation="MSIRVAKTGNEAVAEGMRQINPDVVAAYPITPQTEIVQIFSSFV
                     ANGQVDTEFVTVESEHSAMSATVGAASAGARAMTATSANGLALMWEIVYIAASMRLPI
                     VMPVVNRALSGPINIHCDHSDTMGARDSGWVQIFSENAQEAYDNLLQAVRIAENKNVL
                     LPVMVTMDGFIISHAMEVMHLLEDEKVREWVGEYVPENPLLDVDNPITVGPLDLYDWN
                     MEHKRQQAEAMIQSKPVILQVAKDYAKLSGREYGLFEEYMLDDAEIAIVVLGSTAGTA
                     KEVVKALRADGVKAGLLKVRVFRPFPACEISRALQGKKAIAVMDRSDSFNAVSGPVFA
                     EVRSAMYGMENAPTVVDYIYGIGGRDVTMDHIKSVYDDLAKIVKTGQVE"
     gene            complement(1166..1495)
                     /locus_tag="PLM74_07035"
     CDS             complement(1166..1495)
                     /locus_tag="PLM74_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HON42864.1"
                     /translation="MSVPKDAGWKDVPKAGRILEPGCAAKYETGGWRTYRPVHVPENC
                     INCLFCWIYCPDMSIQVEDGKFKGIDYRYCKGCGVCAQVCPGKRGNKALTMVLDDEAQ
                     GKDDETK"
     gene            complement(1495..2070)
                     /locus_tag="PLM74_07040"
     CDS             complement(1495..2070)
                     /locus_tag="PLM74_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008402650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HON42865.1"
                     /translation="MAKLLEIRWHGRAGQGAKTAALVLAEAVISTGRHAQAFPEYGPE
                     RMGAPMQAFNRISDESITIHSHVERPKIVVVLDPTLMSAVDVKAGVPDDGIYLVNTPL
                     SAVEIRKKLKLENGKAKVYTLDATEISIDTIGRPIPNTPMMGALVAATGLLTLDELIA
                     DTRVKLAKKLRPEIVDKNIEAIRRAYQEVAQ"
     gene            complement(2242..3501)
                     /gene="glyA"
                     /locus_tag="PLM74_07045"
     CDS             complement(2242..3501)
                     /gene="glyA"
                     /locus_tag="PLM74_07045"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HON42866.1"
                     /translation="MFDHVRREDPELARAIFDEIRRQNAKIELIASENFVSRAVLEAL
                     GTPLTNKYAEGYPGKRYYGGCEYVDVAESLAIERAKRLFGADHANVQPHSGAQANTAV
                     YFAALTPGDVVLGMNLTHGGHLTHGMHLNLSGKYFRFIPYGVRSDDQRIDYDELERLA
                     HEHAPKMIVAGASAYPRTIDFERIGKIAKSVGALLMVDMAHVAGLVATGLHPDPVPHA
                     DFVTTTTHKTLRGPRGGMILCTAEWAKKIDSAVFPGTQGGPLMHVIAAKAVAFGEALK
                     PEFADYQKQIVANAKALADALMEHGWKLVSGGTDNHLMLMDLRGTGITGKAAEEALDS
                     VGITVNKNTVPFDPEKPFITSGVRIGTPAVTTRGMKEPQMRVIASLIDGTLRHIGSPD
                     ALAQVSRQVDELVADFPLYPELVAASE"
     gene            complement(3506..4396)
                     /gene="pdxS"
                     /locus_tag="PLM74_07050"
     CDS             complement(3506..4396)
                     /gene="pdxS"
                     /locus_tag="PLM74_07050"
                     /inference="COORDINATES: protein motif:HMM:NF003215.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HON42867.1"
                     /translation="MHALEDSAYAVKVDFINRLKGGVIMDVTTPEQARIAEGAGAVAV
                     MALERVPADIRATGGVARMADPVIVEAIKNAVRIPVMAKARIGHFVEAQVLEALGVDC
                     IDESEVLTPADERFHIDKHVFATPFVCGCRNLGEALRRIGEGAAMMRTKGEAGTGDIV
                     EAVRHMRQVNEDIRRLHSMPQEELMTAAKEMGAPYDLVLWVAREGKLPVANFAAGGVA
                     TPADAGLMMQLGADGVFVGSGIFKSENPQARAEAIVQAVAHYNDPKVLARVSKGLGEA
                     MRGIDASSMSEAEKIQSRGW"
     gene            complement(4617..4787)
                     /locus_tag="PLM74_07055"
     CDS             complement(4617..4787)
                     /locus_tag="PLM74_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019107455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HON42868.1"
                     /translation="MAYKINDECIACGACKPECPTDAISEGDIYVIDPDKCIDCGTCN
                     EVCPVGAPNPEE"
     gene            complement(4937..5012)
                     /locus_tag="PLM74_07060"
     tRNA            complement(4937..5012)
                     /locus_tag="PLM74_07060"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4977..4979),aa:Ala,seq:cgc)
     gene            complement(5059..5790)
                     /gene="nagB"
                     /locus_tag="PLM74_07065"
     CDS             complement(5059..5790)
                     /gene="nagB"
                     /locus_tag="PLM74_07065"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HON42869.1"
                     /translation="MRILVYDDYAQMSAAAARHIASRVNARPSIVLGLATGSTPVGMY
                     AELVELHRREGLDFSRVTTFNLDEYCGLDADDPRSYERFMWEHLFAHVNLDRARTHVP
                     YGAARDPEAECVRYEEMVRAAGGIDLQVLGIGMNGHIGFNEPGSAPNSRTRVVRLAEQ
                     TVQVNRAKTEAEELPSFALSMGIGTIMDAREIMLLANGDAKADIVRQAIQGLITSDVP
                     ASILQQHPDVSVFLDRPAARLLKLD"
     gene            complement(5804..>6317)
                     /locus_tag="PLM74_07070"
     CDS             complement(5804..>6317)
                     /locus_tag="PLM74_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HON42870.1"
                     /translation="RQRTGFDFTSMSHEEARAACRRMGLDVPEHATRGLCLSEAYEEK
                     VEAGLIQPTFIMDYPIEVSPLAKKRADNPELTYRFEAVCAGRELANAFSELNDPIDQR
                     ERFLAQVAERERGDDEAHMMDEDFVTALEYGLPPTGGMGLGVDRLVMLLTDSASIRDV
                     ILFPLMRPRE"
BASE COUNT         1231 a         2017 c         1666 g         1403 t
ORIGIN      
        1 tctcaacctg ccccgtcttg acgatcttgg ccaggtcgtc atagacagac ttgatgtggt
       61 ccattgtcac gtcgcggccc ccgattccgt agatgtagtc gacgacggtg ggggcgttct
      121 ccattccata catcgccgat cttacctctg cgaataccgg cccgctcaca gcgttgaagg
      181 aatccgagcg gtccatgacg gcgatggcct tcttgccttg cagggctctg gatatctcgc
      241 acgccgggaa tggcctgaag acgcgcactt tgagcagacc ggccttcaca ccgtcggctc
      301 tcagggcctt aacgacttct ttggccgtgc ctgcagtcga acctagcacc acgatggcga
      361 tctcggcgtc atccagcatg tactcttcga acagcccgta ctcgcgtccg ctcagcttgg
      421 cgtaatcctt ggccacttgc aggataaccg gcttagactg gatcatcgct tcagcctgct
      481 gcctcttgtg ctccatgttc cagtcgtaca gatccagcgg gccaacggtg atagggttgt
      541 ccacatcaag cagtgggttt tcgggcacgt actcgccaac ccactccctg actttctcat
      601 cctccagcag gtgcatgact tccatggcgt ggctgatgat gaagccgtcc atggtgacca
      661 tcaccggaag cagcacgttc ttgttctcag cgatcctgac cgcctgcaga aggttgtcgt
      721 aagcctcctg cgcgttttcg gagaagatct gaacccagcc tgagtctctc gctcccatgg
      781 tgtcgctgtg atcgcagtgg atgttgatgg ggcctgaaag cgccctgttg accactggca
      841 tcactatcgg cagccgcata gacgcggcga tgtagacgat ttcccacatc agggccagac
      901 cattggctga tgtggctgtc atcgctctgg cgccggcaga cgcggcgccc accgtagcgc
      961 tcatggcaga atgctcgctc tcgacagtaa cgaactccgt gtctacctgg ccgttagcga
     1021 cgaagctgga gaagatctgc actatctccg tctgcggcgt gataggatac gcggctacca
     1081 catccgggtt gatctggcgc atcccttcgg cgacggcctc attgcccgtc ttggcaactc
     1141 taatgctcat tcctgctcct ccttcctact tcgtctcgtc atctttgccc tgggcttcat
     1201 cgtccagaac cattgtgagg gccttatttc cgcgcttgcc ggggcatacc tgggcacaca
     1261 ccccgcagcc cttgcagtaa cggtagtcga ttcctttgaa cttgccgtcc tcgacctgga
     1321 tcgacatgtc aggacagtag atccagcaga acaggcagtt gatacagttt tccggcacat
     1381 ggaccgggcg atatgttctc catccgcccg tctcgtactt cgcggcgcag ccaggctcca
     1441 ggatcctacc cgccttgggc acgtccttcc agcctgcgtc tttcggaact gacattattg
     1501 agccacctcc tggtaggccc gtcgtatcgc ctcgatgttc ttgtcaacga tctcagggcg
     1561 cagtttcttc gcaagcttga ccctggtgtc ggcgatcagc tcatcgaggg tcaacagtcc
     1621 tgtggcagcc acgagcgctc ccatcatggg agtattggga atcggccgcc ctattgtgtc
     1681 gatggatatc tctgtagcgt cgagagtgta caccttcgcc ttgccgtttt ccagcttcag
     1741 cttcttgcgg atttccacag cagacagcgg tgtattaaca aggtagattc catcgtcagg
     1801 aaccccggcc ttcacatcaa ctgcagacat gagtgtgggg tcgagtacta cgacgatttt
     1861 cgggcgttct acgtggctgt gaatggtgat tgattcgtcg ctgattctgt tgaacgcctg
     1921 catgggcgcg cccatgcgct ccgggccgta ttcaggaaaa gcctgtgcat gccttcctgt
     1981 ggagatgacc gcttcggcaa gtaccaaagc agccgtcttc gctccctgcc cggcgcgtcc
     2041 atgccagcga atctccagga gcttagccat gatgtttctc cccctccgtt ctgttgggcc
     2101 atacgtgact gaccggactc aaatgggatc agcctgtaca gctatatgga tcatgccgcg
     2161 gaagcccgcg gcatggatca tctcaagtcg acatcgaccg tgcttgacta tgccgtgacg
     2221 cccctcacaa aacgccagcc gctactcgct tgcggcgaca agctcggggt agagcggaaa
     2281 atcggcgaca agctcgtcta cctgccgcga cacctgggcc aacgcatcgg gagatccgat
     2341 gtgccggagc gtcccatcga tcagcgacgc aatcactcgc atctggggtt ccttcatgcc
     2401 gcgcgtggta acggcgggag tgccgatcct gacaccgcta gttatgaacg gcttttccgg
     2461 gtcgaacggc actgtgttct tgttgacagt gatgcccaca gaatccagcg cctcttcggc
     2521 agccttgccc gtaatgcctg ttccacgcag atccatcagc atcaggtggt tatcggtgcc
     2581 gcccgagact agtttccatc catgctccat gagcgcatca gccagagcct tggcattggc
     2641 gactatctgc ttctggtagt ccgcgaattc gggctttaga gcttccccga acgccaccgc
     2701 cttggcggcg atcacatgca tgagcggccc gccctgagtg cccgggaaga ccgcagagtc
     2761 tatcttcttc gcccactcag cagtgcagag tatcatgccg ccacgaggtc cgcgcaaggt
     2821 cttgtgcgta gtggtggtca cgaaatcggc atgcggaaca gggtcaggat gaagcccggt
     2881 ggcaacgagt ccggcgacgt gggccatgtc taccatgagg agcgctccca cgctcttggc
     2941 gattttgccg attctctcga aatcgatagt cctcgggtac gcactggccc cggccacaat
     3001 catcttgggc gcgtgctcgt gggcaagcct ctccagctca tcgtagtcga ttcgctgatc
     3061 gtcggacctg acgccatacg ggatgaacct gaagtactta ccggataagt tcaaatgcat
     3121 gccgtgggtc agatgtcccc cgtgtgtcag gttcatgccg agcacaacat cgcctggcgt
     3181 cagtgccgcg aagtagaccg ccgtgttggc ttgagcccct gaatgcggct gaacgtttgc
     3241 atgatccgct ccgaacagcc tcttcgcccg ctcgatagcc aggctctcag ctacatcgac
     3301 atactcgcac ccgccgtagt acctcttgcc cgggtatcct tcggcgtact tgtttgtcag
     3361 cggcgtaccg agggcttcaa gtaccgccct gctcacgaaa ttctccgacg caattaactc
     3421 aatcttcgca ttctgcctgc gaatctcatc gaaaatcgcg cgcgccagtt cagggtcttc
     3481 ccgcctgaca tgatcaaaca tcgctctacc agcccctact ctgtatcttc tctgcctcgc
     3541 tcatcgagga ggcatcaatc ccccgcatcg cctcgcctag cccttttgat acccgtgcaa
     3601 gcaccttggg atcattgtag tgagcgaccg cctgaacgat ggcctcagcc ctcgcctggg
     3661 gattctccga cttgaatatg ccggagccga cgaacacgcc gtccgccccg agttgcatca
     3721 tcaaacccgc atcagcaggc gtagcgactc cacccgcggc aaagttggcc acaggtagct
     3781 tgccttcccg cgccacccac aaaactaggt cgtaaggcgc ccccatctcc tttgcggcag
     3841 tcatcagctc ttcctgaggc atgctgtgga gtcgtcggat atcctcgttg acctgcctca
     3901 tgtgccgcac tgcctctact atgtcgcctg ttcccgcctc gcccttagta cgcatcatcg
     3961 cagcgccctc gccgatacgg cgcagcgcct cgcctagatt ccggcatcca cacacgaaag
     4021 gggtcgcaaa aacgtgctta tcaatgtgga atctctcatc ggccggcgtg agaacctcgc
     4081 tctcgtcgat gcaatcgacg cccaaagcct cgagcacctg cgcctcaaca aaatgcccta
     4141 tgcgcgcctt ggccataacg ggtatgcgca ccgcgttctt gattgcttct acaatgacag
     4201 gatcggccat tctggcgaca ccgccggttg cacgaatgtc agcaggaacg cgctcgaggg
     4261 ccataacagc aacagcaccc gcgccttcgg cgattctggc ctgttccggc gttgtgacat
     4321 ccatgatcac tccgcccttt agcctgttta tgaagtccac cttgaccgcg taggcgctat
     4381 cttccaacgc atgcatactc atgagactac aacctcccaa gcgccgaaat ccaacattaa
     4441 ttctactaca ctgccgctgc atgcacaacc ttcaccaggg gccagatgga tacccggcct
     4501 ctcgctgtat gcattcgcgg tacggccgac tgcgcgtgca cttccataca caaatcaatg
     4561 gactcctcta cacatggtga ggagcccatt gcccgaaaag cgcagtaata cggttgtcac
     4621 tcctcagggt tgggcgctcc cacagggcac acctcattgc aggtgccaca atcgatgcac
     4681 ttatcgggat cgatcacgta gatatcgccc tcgctgatgg cgtcagtggg gcactcaggc
     4741 ttgcacgccc cgcaggcaat gcactcatca ttgatcttgt acgccatttt aggccctcct
     4801 tccgaggtat aatacctgcc ctattgtaca cgatgaaatg acgcttaaca atggcttttg
     4861 ttacatgatt cttgcctgag ataaaaagcc cggcgaaagc aatgcctccg ccggatacat
     4921 caaggtatag actgattggt ggacccgacg cggatcgaac gcgcgacctc tagagtgcga
     4981 ttctagcgct ctcccagctg agctacgggc ccatggaact tgcggcaact gttatgttac
     5041 cacaccagac agcgtgcgtc aatcaagttt gagcagcctg gccgcaggtc tatcaagaaa
     5101 gacagacacg tccggatgct gctggaggat ggatgcagga acatcggagg taattagccc
     5161 ttgaatagcc tgccggacta tgtcggcttt ggcatccccg ttggccagca gcattatctc
     5221 gcgcgcatcc attatcgtgc ctatccccat cgacagtgcg aatgacggca gttcctccgc
     5281 ctcggtcttg gccctgttga cttgcactgt ctgctctgcc aaccgcacaa cgcgcgtgcg
     5341 ggagttcggc gcggacccgg gttcattgaa cccgatgtgg ccgttcatcc ctatgcccaa
     5401 tacttgcagg tcgattccgc ccgccgcccg gaccatctct tcatatctga cgcactcggc
     5461 ctcgggatcg cgagccgccc cataaggcac gtgtgttcgg gcgcggtcca gattcacgtg
     5521 ggcgaacaga tgctcccaca tgaaccgctc gtagcttcgg ggatcgtcgg catcgaggcc
     5581 acagtactcg tcaaggttga acgtagtgac acgggaaaag tccagcccct cccgccggtg
     5641 gagctcgact agttccgcgt acatcccgac cggggtcgat ccggtagcca gccccagcac
     5701 aatactgggc cgggcgttca cgcgagacgc aatatgcctg gccgcggcgg cgctcatctg
     5761 ggcatagtca tcgtagacga gtatgcgcac accaatcgcc tcgctattcc ctcgggcgca
     5821 tcagagggaa gagaataaca tccctgatcg aagccgagtc ggtcaggagc atgacgagcc
     5881 tgtccacacc taggcccatc ccgcctgtag gcggcagacc atactccaag gcggtgacga
     5941 agtcctcatc catcatgtgc gcctcgtcat ctccccgctc cctctcagct acttgggcca
     6001 ggaagcgttc acgttgatcg attggatcat tcagctccga gaacgcgttg gccagttcgc
     6061 gaccggcgca caccgcctcg aacctgtagg tcagctccgg gttatcggcc ctcttcttgg
     6121 ccaggggcga cacctcgatt gggtagtcca ttataaaggt aggctggata agacccgcct
     6181 cgaccttctc ctcatatgcc tcgctcagac atagcccacg agtagcgtgc tccggaacat
     6241 ccagccccat tcggcggcat gctgccctgg cttcttcgtg actcattgac gtgaaatcga
     6301 agcccgtacg ctgcctt
//
LOCUS       DAOTAC010000141         6316 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_10446_length_6316_cov_3.059096, whole genome shotgun sequence.
ACCESSION   DAOTAC010000141 DAOTAC010000000
VERSION     DAOTAC010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6316
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10446_length_6316_cov_3.059096"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            30..584
                     /locus_tag="PLM74_07075"
     CDS             30..584
                     /locus_tag="PLM74_07075"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HON42871.1"
                     /translation="MPRINLLPERETRKAPVSPKRLFFIIVVEVAVVAALTVFLSKVF
                     TIRQMEAELAQVRAQIAAMQQDLDILDQVQTRTKEIRALADEIRSIQAENRAPAPVLR
                     ELRAVVPQDVWLESFSADPKGAISLGGATFSMESAARLSLQLERSPVFADVKLSSVTL
                     GSRGESQVYTFNIGCALNAVGAGR"
     gene            591..1226
                     /gene="pilO"
                     /locus_tag="PLM74_07080"
     CDS             591..1226
                     /gene="pilO"
                     /locus_tag="PLM74_07080"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HON42872.1"
                     /translation="MSACDHIDRQMRLLGVLIIVLAAAGLYLYVYSPQQRKHERLKAE
                     LDMANSELAIGLVKMRKAPEAEGELEAAVAGLEALMRLIPAEDKLNWIVRDIESVARA
                     NRVTVVEVTLAVGKPRGRYLEVPVTVSLTGKYEDVLGFVEGLGKLPRIINVHGFTFEG
                     GASRPGEEAALAESVLPRENLISATVQATTYALAKGGGQVDKPQAKPTSSR"
     gene            1189..1833
                     /locus_tag="PLM74_07085"
     CDS             1189..1833
                     /locus_tag="PLM74_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42873.1"
                     /translation="MISLKRSRPQVARVNLTPGSASAASSVDQQERARERRRAWIPVL
                     ALVVLLGVAVGYFLFMANYRRGALDPDAPPIVAVGGHFAQRFADLLVRSDVAGLVAEG
                     PSLGPGDNGRWDPFKPLVELGPAIKPMVPDTPVVVPVEPEPEPPSPPQVTLTGVLTSG
                     GRAIAILRVAGQTQLTRVGESPFEGAVVRSIDDKSIAISFFGQDIHYSLGGEQQ"
     gene            1830..5918
                     /locus_tag="PLM74_07090"
     CDS             1830..5918
                     /locus_tag="PLM74_07090"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HON42874.1"
                     /translation="MRKAERSGALMCIVAMVVCLALAGRPVEAGSPKVDMDLVGAGIA
                     DVFRALAELGDMNIVLDPAVSGTLTIRLHDLTVEEALKLVAYTTGVEYRVVGSSLIVT
                     PPGKPSAFDPVRVERYSLTHARVDEVMPVLGFVADNTKLQADPRTNSVIARGPVAGLE
                     QVKQVISMLDVAVVPPEAEIPAKGVDAKAGPPSPELPAPTPAPVESLDIVKLRYASAR
                     SVAGLLSLVVPSGKIQADSDINAIVMLADEVALGRAHEIIAQVDVTPAEVPLASAPVA
                     VEVAEPKAPAEADAVKVIKLSWSPADKVAEALRVVVDGVRIGTDSRTNSLVVRAGQSD
                     MLRVEEIASLLDVPVVEPETFALPEAPKPEPRALASFTLVHADPGEVQRALALVVPDG
                     AVHPDARTRSLLVFGTPDEMARAREMIALLDVATPAPVVSPAPAVDQAPKAQEPDTLK
                     AIVLEYADVSVVREALSTVLPSENIIVDQRTSKVIVRGTKAQHRLASEIVAMLDVSDA
                     VRAGATKAAEPAQVKGIQAFRLAQADPIAVREALALVIPRDAVHVDTHTRTALVLGLP
                     DEIAKAAQIVSLLDTAVAPALAPEPVAEPVSEPPAPVRTVHAFRLNQADPAAVREALA
                     LVLPRDAVHVDTRTRTAIVLGLPDDIATASEIVGLLDEAAAPEQQPKPELPVDVMRVI
                     RLKHAAAVQIREAIVSAVPGARLTADPRTNALIVVAPGDVQVKVDDLVAALDVEAEPL
                     AAAATAAPRELAPKPEPETAEVVRLEYAAPAAVRQALLPTLPESKMSVDERTAAIVIV
                     GTAAQREQARRIIAQLDVEVPPSPDEIAAPTAPADPEVLQVFQLAHAPASRVREALAP
                     VVPVASMTVDERTNSLIINASRSRVTRAAEVIERLDVEIKPASSEAAAPTPQPEAEVA
                     RYRLANAPAESLRSAVSLLVASSDIQVDSRTNTLLVRAVPGVQQSVAELVQGLDIPVE
                     PAPEPKPEPTPATAPPEVMKVFRLTHAAASDMKTLLGIAAPSAKMQPDDRTKSLIVMA
                     PASTLAALDELVRAMDIPAAPSVQANAQPAPEPDPVVTRVYRLNHAVAGEVAKALEGL
                     VKGAVKADVRTSSLVVEAAESQHARALSLIEALDRELPQVLIEARLEELSGDAGRRLG
                     LDWSFNGLTFNQNAYGQWVSVSLDFLTNLTALEEQGKASLISRQHTFTVSGKTGKILI
                     GDRIPVMIQEVQDGVAVTKLDFINAGIELSITPIVSEDGTITATVKPVISSIVGWTPQ
                     NYPQIRTRELETIVSLKSGNTAVIGGMLHRDEIESLAKVPILGDIPLLGELFKKRSTT
                     TENTQIVVMITAWQVNPGQHPVTGPATGDDKFPVKLEGAPTK"
     gene            6079..>6316
                     /locus_tag="PLM74_07095"
     CDS             6079..>6316
                     /locus_tag="PLM74_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003249229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HON42875.1"
                     /translation="METKLGKLRALFADNGIDGLIITGPENRYYMSGFSGSAATLVIS
                     ADSAALITDFRYVEQANAQAPEYRVYMHKNPMTDT"
BASE COUNT         1196 a         1801 c         2121 g         1198 t
ORIGIN      
        1 ggcctggctc ttcgggaggt ggttgattaa tgcccaggat caacctgctg ccggagcgtg
       61 aaaccagaaa ggcgccggtc agtccgaagc ggctattctt catcattgtc gtggaggtcg
      121 cagtcgtcgc cgcgctcaca gtctttctga gtaaggtctt cacaatacgc cagatggaag
      181 cggagcttgc gcaggtgagg gcgcagattg cggcgatgca gcaggaccta gacatactcg
      241 atcaagtgca gacgcgtact aaggagatcc gcgcgctcgc cgacgagatc cggtctatcc
      301 aggctgagaa ccgggcgcct gctcccgtcc tgcgcgaact gcgagctgtc gtgccgcaag
      361 atgtatggct ggaatcattc tcagccgacc cgaaaggggc cataagcctt ggcggggcca
      421 cgttctcgat ggaatcggcg gcccgtcttt cactgcagct ggagcgctct cctgtgttcg
      481 ccgatgtcaa gctgagttcg gtcacgcttg ggtccagggg cgaaagtcag gtgtacacgt
      541 tcaacatcgg ctgtgccctt aacgcggtcg gggcggggag gtgagccggg atgtctgcgt
      601 gtgaccacat tgaccggcag atgcggcttt tgggcgtgct cataatcgtc ctggccgcgg
      661 caggccttta tctgtacgtt tactcgcccc agcagcgcaa gcatgagcgg ctcaaggccg
      721 agctagatat ggccaactcc gagcttgcca taggccttgt gaagatgcgc aaagcgcccg
      781 aagccgaagg ggagctcgaa gcggccgtcg cagggctcga agcgttaatg cgcctgatcc
      841 cggcggagga caagctcaac tggatcgtcc gtgacattga gtcggtggcg cgggccaacc
      901 gggtcactgt tgtagaggta acgctggcag tcggcaagcc gcgcgggcga tacctggagg
      961 ttccggtgac ggtgtccttg acgggcaagt atgaggatgt gctcggtttt gtggaaggcc
     1021 tcggtaagct gccgaggatc atcaacgtgc acggcttcac cttcgaaggc ggcgcgtcac
     1081 gcccggggga agaggcggct ttagccgagt ctgtcttgcc ccgcgagaac ttgatctccg
     1141 ctacggtgca ggccacgacg tatgctctgg cgaagggggg tgggcaggtt gataagcctc
     1201 aagcgaagcc gacctcaagt cgctagggtc aacttgacgc cgggctccgc gagtgccgcg
     1261 tcgtccgtcg accagcagga acgggcgagg gagcgccgac gcgcatggat accggtgctg
     1321 gccctggtgg tgctgctcgg ggttgcggtt gggtacttcc tcttcatggc gaattacagg
     1381 cgcggtgctc tggaccctga tgcgccgccg atagtggctg tgggaggcca tttcgctcag
     1441 agattcgccg acctgctcgt ccggagcgac gtggcggggc tcgtggctga aggaccgtcg
     1501 cttgggcccg gcgacaacgg tagatgggat ccgttcaagc ccctggtgga attgggaccg
     1561 gctatcaagc ccatggtgcc cgatacacct gtggtcgtgc cggttgaacc cgagcctgag
     1621 ccgcccagcc cgcctcaggt cacgctcaca ggagtgctga cgtcgggggg acgggcaatc
     1681 gcgatccttc gggtcgccgg gcaaacacag ctgacccggg tgggcgagtc tccgttcgag
     1741 ggcgctgtgg tgaggtccat agatgataag tccattgcga tatcgttctt tggccaagac
     1801 attcattact ccctgggggg tgagcaacaa tgagaaaggc tgaacgctca ggcgctttga
     1861 tgtgcattgt ggcaatggtg gtctgcctgg cgctcgcggg ccggcctgtg gaggcgggat
     1921 cgcccaaagt cgatatggat ctcgtcggcg cgggcatcgc tgacgtgttc cgggccctgg
     1981 ctgagctggg cgatatgaac atagtgctcg acccggctgt ttcaggcacg cttactattc
     2041 gcctgcacga cctgactgtg gaagaggccc tgaagctagt tgcctacact acgggcgtgg
     2101 agtatcgcgt ggtgggttcg agcctgatcg tgaccccgcc cggaaagccc agcgccttcg
     2161 accctgttcg ggtcgagcgg tactcgctca ctcatgccag ggtagacgaa gtgatgcccg
     2221 tgttggggtt cgtggccgat aacaccaaac tgcaggctga tccgaggacc aactcggtga
     2281 ttgccagggg ccccgtggcc ggcctggagc aggtgaagca ggtcatatcc atgcttgatg
     2341 tggcggtcgt gcctccggag gccgagatac cggctaaagg cgtggatgcg aaagccgggc
     2401 cgcctagtcc ggaacttccc gcgccaactc cggctccagt cgaatccctc gatatcgtaa
     2461 aactcagata tgcgtcggcc aggtccgtgg caggccttct gagcctggtg gtaccgagcg
     2521 gcaagattca ggcagactcc gatatcaacg ccatagtgat gctggcggac gaggtcgcgc
     2581 tgggcagggc ccatgagatc atcgcgcagg tggacgttac gccggctgaa gtgccgctcg
     2641 cgtccgcgcc tgtggcggtc gaggttgctg agccgaaggc gccggcggag gcagatgcag
     2701 ttaaggtcat taagctttcg tggtcgccgg cggacaaagt cgctgaggct ctgcgtgtgg
     2761 tggttgacgg tgtgaggatc ggcacggact cccgcaccaa ctcgcttgtg gtgcgcgccg
     2821 gccagtccga tatgttgcgt gtggaggaga tcgcatcgct tctggacgtg ccggtggtgg
     2881 aaccggagac gtttgcgctc cctgaggcac ccaagcctga accgcgggcg ctcgcatcat
     2941 tcactcttgt tcacgcggat ccaggtgagg tgcagagggc gctggctcta gtcgttccgg
     3001 acggggcggt acaccctgat gcgcgaaccc gctcgcttct ggtgttcggc acgccggatg
     3061 agatggcgcg cgcgcgtgag atgattgcac ttttagacgt ggccactccc gctccggtgg
     3121 taagccctgc ccctgccgtg gaccaagctc ctaaggcgca ggaaccggat actctgaagg
     3181 ccatagttct ggagtatgcg gatgtctcgg ttgtgcggga agcactcagt accgtgttgc
     3241 cgagcgagaa catcattgtg gaccaaagaa cctccaaggt cattgtgcgg ggcacgaagg
     3301 cgcagcaccg gctggcttcg gagatcgtcg ccatgctaga tgtgtcggat gccgtaaggg
     3361 ccggcgcgac gaaggcggca gagcctgcgc aggtgaaggg cattcaggcg ttccgcctgg
     3421 cccaggcaga cccgatcgcc gtacgtgagg cgctggcttt ggtgatacct cgcgatgcag
     3481 tccacgtcga cacgcatacg cgtacagcgc tggtcctggg gttgcctgac gagatagcca
     3541 aggcggccca aatagtatcg ttgctcgaca cggctgtggc gccggcgctg gctcctgagc
     3601 ctgtggcgga accggtgtct gagccgcccg ctcctgtgag gacggttcat gcatttcgtc
     3661 tcaatcaggc tgacccggct gctgtgcggg aggcgctggc cctggtgctg ccgcgcgatg
     3721 cggtgcatgt ggatacgcgc actcggacgg cgatagtcct ggggctacct gatgatattg
     3781 ccacggcttc cgagatagtc gggttgcttg atgaggctgc ggcgcctgag caacagccta
     3841 agccagagct gcccgtagac gtgatgcgcg tgatcaggct taagcatgct gcggcggtcc
     3901 agatccggga ggcaattgtg tctgctgtac cgggcgcccg actgacagcc gatcctcgca
     3961 cgaatgccct gatcgttgtg gcgcccggcg atgtgcaggt caaagtggac gacctggtgg
     4021 ccgcgcttga tgtcgaggct gaaccgcttg cggcggctgc tactgccgca ccccgggagc
     4081 tggctccgaa accggaaccg gagacggcag aggtggttcg actggagtac gcagcgccgg
     4141 cggcagtgcg ccaggcgctc ctgcccacat tgcctgaatc gaagatgagc gtggatgaac
     4201 ggaccgctgc catcgtaata gtgggcacgg cggcacagcg cgaacaggct aggcggatca
     4261 ttgcccagct ggatgtggag gttcccccgt cgcccgatga gatcgcggcg cccactgcgc
     4321 cggccgatcc cgaagtattg caggtgttcc agctggcgca tgcaccggcg tcacgtgttc
     4381 gggaggcgct ggcccctgtg gtgccagtcg ccagcatgac cgtggacgag cgcaccaact
     4441 cgctcatcat caatgcgtcg cgatcgcgcg tcacgcgggc cgctgaggtc atagaaaggc
     4501 tcgacgtgga gatcaagccg gcgtcgagcg aagcggctgc gccaacgccg cagcccgaag
     4561 cggaggtggc acgctaccgg ttggcgaatg ctcctgcgga gtcgctgcgg tcggctgtga
     4621 gtctcctggt cgcctcttcc gatattcagg tggattccag gaccaacacg ctgctggtgc
     4681 gtgccgttcc aggtgtgcag cagagtgtgg ccgaacttgt tcaggggctc gacattccag
     4741 ttgaacctgc gcccgagcct aagcctgagc cgactcctgc tacggcgccg cctgaggtga
     4801 tgaaggtatt ccgcttgaca cacgcagcgg cgtccgacat gaagacgctt ctgggcatag
     4861 cggcgccgtc ggccaagatg cagccggacg acaggaccaa atcgctgatt gtgatggcgc
     4921 ccgcgtctac tctggctgct ctggatgaac ttgtgcgagc catggatatc cccgcagcgc
     4981 ccagcgtaca ggcgaatgcg cagccggcac ctgaaccgga tccggtggtg acccgggtat
     5041 acaggctcaa ccatgcagtt gcgggcgagg tggcaaaggc cctcgaaggg ctggtgaaag
     5101 gcgcggtaaa ggccgatgtg cgcacgtctt cactggtagt cgaggccgca gaatcacagc
     5161 atgccagagc attgagcctc atcgaagcac tcgataggga gcttccgcag gtgctgatag
     5221 aagcgaggct ggaggagctg tcgggcgatg cgggccgaag gctcgggctg gactggtcct
     5281 ttaatggtct caccttcaac cagaacgcct acggccaatg ggtgagcgtg agcttggatt
     5341 tcctgacgaa ccttaccgcc ctggaagagc agggcaaagc gtcgctgatc agccggcaac
     5401 atacgttcac cgtcagcggc aagaccggca agatactgat aggcgacagg atccccgtga
     5461 tgatacagga ggtccaggac ggcgtggccg tcaccaagct ggactttatc aacgccggca
     5521 ttgagctttc gatcaccccg attgtaagtg aagacgggac gatcacggcc acggtgaagc
     5581 cggtcataag ctcgattgtg gggtggacac ctcagaacta cccacagatt aggacgaggg
     5641 aactcgaaac catagtgtcg ctcaagagcg gaaacaccgc tgtcatcggg ggcatgctgc
     5701 accgcgatga gatagagagc ttggcaaagg ttccgatcct cggcgatatc ccgcttcttg
     5761 gggagctttt caagaagcga agtactacta ctgagaacac ccagattgtg gtaatgatca
     5821 ccgcctggca ggtgaacccg ggtcagcacc ccgtgactgg cccggcgacc ggtgatgata
     5881 agttcccagt caagctagaa ggcgcgccga ctaagtaaga aggattcgac gcgctagcca
     5941 gcatttctag gcgtacccat tcggaggacg gtctccgcac caaggcgacc gttctccttg
     6001 ttgttcggac gggcgcttcc caattcttgc ccggcattag aggattgtgg tagagtaatc
     6061 tgtgggagga atgagcgagt ggaaacgaaa ctcgggaagc tacgtgcact ctttgccgat
     6121 aacggcatcg atggactgat catcacgggg cccgagaacc gctactacat gagcggtttt
     6181 tccgggtcgg ccgcgaccct agtcatcagc gccgacagcg cggcgctcat caccgacttc
     6241 aggtatgtgg agcaggcaaa tgcacaggct cccgagtacc gtgtgtacat gcacaaaaac
     6301 cccatgaccg acacct
//
LOCUS       DAOTAC010000142         6304 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_10468_length_6304_cov_4.755481, whole genome shotgun sequence.
ACCESSION   DAOTAC010000142 DAOTAC010000000
VERSION     DAOTAC010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6304
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10468_length_6304_cov_4.755481"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="PLM74_07100"
     CDS             <1..307
                     /locus_tag="PLM74_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018467957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HON42876.1"
                     /translation="MFYTGEGRTLDKPVFLQFPGDPHLAGKRILVVDDVWDSGRTIVA
                     VRERIERVGGEPRTAVLHYKPTHSAFPDLSPDYHAAEVDGWIVYPWDPSRLAANVMQ"
     gene            381..1475
                     /locus_tag="PLM74_07105"
     CDS             381..1475
                     /locus_tag="PLM74_07105"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HON42877.1"
                     /translation="MKVGIVGMPWAGKTTVFRLLTGGNPGGKQDASIGMAKVPDARID
                     VLSEMFRPRKTTYAQIEVVDLAGGAGLGLGRIAADDNGRARARGISLLVNHMRTVDAL
                     IHVVRTFESADGEPARPVADAASLNDELILADMAIAEARVERLRLSRKRTSDEDRELE
                     LMELLAAEFVEGRGLREIELAKGDQDLLRGYGLLSVKPVVLAANMSEEQFQSGGFVDE
                     IQLQGYCDERHIPYVRLCASIEADISELPPDERIAFMDAYGIESTGVERVSRAVYECL
                     GLVSFFTVGEDEVRAWPIAVGTNAKHAAGKIHSDIEHGFIRAEIVAYDDLIRLGSYSA
                     ARAAGAFRLEGREYTMADGDIVNFRFSPPR"
     gene            1607..1822
                     /locus_tag="PLM74_07110"
     CDS             1607..1822
                     /locus_tag="PLM74_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42878.1"
                     /translation="MVQTQGITPTENWFAHEQLSKEQLCMKKCSIYSNQIQDPQLKGS
                     VQRLMDSCQRHVNALQGIVGQTGQTKM"
     gene            1855..2112
                     /locus_tag="PLM74_07115"
     CDS             1855..2112
                     /locus_tag="PLM74_07115"
                     /inference="COORDINATES: protein motif:HMM:NF019490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HON42879.1"
                     /translation="MDLQGKFTDKDIASDLLSDLKEMLLGYHIATVESASPNVRNTFM
                     NSHDDYMKEQRELWETMYSKGWYQIVGQPQQPGYHSPQGRM"
     gene            2293..2964
                     /locus_tag="PLM74_07120"
     CDS             2293..2964
                     /locus_tag="PLM74_07120"
                     /inference="COORDINATES: protein motif:HMM:TIGR01488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HON42880.1"
                     /translation="MRLIAFDMDGTLTDNPSSWEHIHRRFGLWEAHADAYMKRFLAGE
                     ITYAEFSRLDALEWKGKPRRQLDAVLDEIGYAPCAREAVSAAREAGASVALVSSGLSF
                     LASRVARELGICHVFANELAVCDGLMTGEVVINVSIDDPARTKAAILKQLRSALRADR
                     AETWAVGDNWGDIEMLQEAGRAFLVAPRPECWEQAMQAAPHVECIADLCHLVSLIRNV
                     SVCAD"
     gene            complement(2914..5061)
                     /locus_tag="PLM74_07125"
     CDS             complement(2914..5061)
                     /locus_tag="PLM74_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON42881.1"
                     /translation="MNGYSNEEEIVGKAYDAKLMRRLLRYARPYWAIFAAALVVLMVI
                     AAADLVRPYVIKIAIDEYVTGYENPMARFEPDQVPADVREQHRMHYAGSVFVRSEHLP
                     VEMRHETHYQMLAVAGKYVIIDKVLPPEHGQAELVETAEGAVLHVDSLDYAAEIVDSA
                     DAVREFRGRDIEAIGRLALLLLVVGMGAFALNYGQTVTLHYVGQRIVYNIRQEVFTHI
                     SHLDLAFFDRSPVGRLVTRLTNDTETLNEMYTSVLVNLFKDVFMLIGIIAVMFRLNTQ
                     LALVSMATLPLIALVTVVFRVKARDAYRRVRAALARINAFMAESISGMRVVQIYNREK
                     KKSDEFAEVNRDYYKAGIGEMMVYAVFRPIVELIGTLALSLLVWYGGRRVITGQMPFG
                     LVYAFINYIGMFFGPINDLTEKYNILQAAMASSERMFQLLDTPITVRDPADPIDIGQV
                     RGDIEFKNVWFAYSNDDWVLRDVSFTIKPGEMVAFVGATGAGKTSVISLMNRFYDIQK
                     GAILVDGIDIRRYRVSDLRKNIATVLQEVFLFTGDIKGNIRLNNTDISDEKIREVAHY
                     VNADHFISRLPHGYDEPVTERGSTLSAGQRQLLAFARALAFDPAILVLDEATANIDTE
                     TELLIQDALPRLIAGRTTIVVAHRLSTIQHADKIIVLHKGRIREMGTHQELLAHKGIY
                     YDLYRLQYKDDFGLPTPSIRADAYVSDEADQMA"
     gene            complement(5097..>6304)
                     /locus_tag="PLM74_07130"
     CDS             complement(5097..>6304)
                     /locus_tag="PLM74_07130"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON42882.1"
                     /translation="GPRFRAVHEAFSDLTERAQESIAGIRVVKAFVQEKAETSRFMDV
                     AQKNVDENMRLIRIWGLMFPMSGLASTLAFVFVLKYGSAMIIRQEISLGDFVALTSYL
                     AMLVWPMIAVGWVINVVQRGYAALDRINKILNEAPEVADEPGAVELPSVAGTIEFRNV
                     TFSYSPELPPALSDVSFTIEAGKTLAVVGRTGSGKSTIVSLLARLYNPPPGSVFIDGH
                     DIRQVTLESLRTQLGVVPQESFLFSTTIGKNIAFASDEYPLERIEHFAEVAQVRKDIS
                     EFPDGFDTMVGERGVTLSGGQKQRICIARALIKEPKVLVLDDCLSAVDTQTEESILRG
                     LREYMRGRTSIIISHRISTVKDADEIIVLDDGVIRERGTHDTLVAAGGLYYELHEKQL
                     LEEELASKS"
BASE COUNT         1324 a         1761 c         1894 g         1325 t
ORIGIN      
        1 gatgttctac accggcgaag ggcgtaccct cgataaacca gtcttcctgc aattcccagg
       61 cgatccgcat ctggccggca agcgcatact ggttgtggac gatgtatggg actcggggcg
      121 gacgattgtg gcagtgaggg agcgtatcga gcgcgtgggc ggagaaccgc gtacagcagt
      181 gcttcattac aagcccacac actcagcctt tcctgacctc agccccgact accatgcggc
      241 agaagtggac gggtggatag tgtacccatg ggatccgtct cgtcttgccg ccaacgtcat
      301 gcaatagacg gcgaaggcgc agcgcacgac gggcataggc agcaactgaa gctacacgcc
      361 gatcgatgga aggtgcagtc atgaaagtag ggatagtagg catgccctgg gccggcaaga
      421 ccacggtctt ccgcctcctg acaggcggta accccggcgg caagcaggat gcgagcatcg
      481 ggatggcaaa ggtgcccgac gctcgcattg acgttctgag cgagatgttc aggccgcgca
      541 agaccacgta cgctcagatc gaagtggtgg accttgcggg aggtgcaggc ctcgggctgg
      601 gtcgaattgc ggcggacgac aatgggcggg cgagggcgcg cggcatttca ctgctcgtca
      661 accatatgcg tacggtggat gctctgatac acgttgtgcg tacgtttgag tcggccgatg
      721 gcgaacccgc acggccggtt gccgatgccg cgtcgctcaa cgacgaactg atactggccg
      781 atatggccat agctgaggca agggtggagc gcctccgatt atcgcgcaaa cggacctccg
      841 atgaagaccg ggaactcgag ctcatggagt tgctcgccgc cgagtttgtt gaaggccggg
      901 ggctcaggga gatcgagcta gccaagggcg atcaggatct cctgcgcggc tacgggttgc
      961 tctctgtgaa acctgtggtc ctggcggcga atatgtcgga ggagcagttc caatcgggcg
     1021 gcttcgtaga cgagatacag ctgcagggct actgtgacga gcggcacatt ccttacgtca
     1081 ggctatgtgc gagcatcgag gccgacatat ctgagcttcc cccggatgag cgtattgcct
     1141 tcatggatgc atacggaatt gagtctacag gagtggaacg tgtgtcgcgc gccgtatatg
     1201 agtgcctggg cctagtgtcg ttcttcaccg taggagagga tgaagtgcgg gcatggccca
     1261 tcgcagtcgg cacaaatgca aagcacgcgg ctggcaaaat acactccgac atagaacacg
     1321 gcttcatccg agccgagatc gtagcctacg atgatctgat acggctggga tcgtactcgg
     1381 cagcccgggc tgccggcgcc ttcaggctcg agggcaggga gtacacaatg gccgatggcg
     1441 acattgtgaa cttccgattc agtcctccgc ggtaggcagc aggaagtggg ctgattcgtc
     1501 ttcgtcctcc tatcgcgaat agcgtcacac gcgtcgagag atattagctt tgatggcctg
     1561 cgcgtgcgcg cgggcgacgc gacgcgatgc gaagggaggt ttgggattgg ttcagacgca
     1621 ggggattacc ccgaccgaga actggttcgc ccacgagcag ctcagcaagg agcagctgtg
     1681 catgaaaaag tgcagcatct actcaaacca gatccaagat ccgcagctca agggcagtgt
     1741 gcagcggctc atggattcat gccagcggca cgtcaatgcc cttcagggca tagtggggca
     1801 gacagggcag acgaagatgt gacggcctga tacgggctga aagggggaat gcgtgtggat
     1861 cttcagggta agttcacgga caaggatatc gcttccgacc ttctgagtga cctcaaggag
     1921 atgctcctcg gctatcacat agccacggtg gaatcggcga gccccaacgt acgaaatacc
     1981 ttcatgaaca gccatgacga ctacatgaag gaacagcgcg agctgtggga gaccatgtac
     2041 tctaagggat ggtatcagat cgtcggtcag ccgcaacagc ctgggtacca ttcgccccag
     2101 ggacggatgt agagccgccc gggcagcatc tgccggctgt cgtcggtctg ctgagttgag
     2161 agggaatgta caagtgcggc cggagtctcg ccggccgttc actttgtaca aggaacggcc
     2221 ggttctccgc cgaaacaaca cctacagtgt ttggggatgg cacttgatgg gagagtgaat
     2281 gagcgattgc aggtgagact aatagccttc gacatggacg gcacgctgac cgacaatccc
     2341 agttcgtggg agcacattca ccgaagattc ggcttatggg aggcacacgc ggatgcctac
     2401 atgaagcgct tcctggcggg cgagatcact tatgccgagt tttcgaggct cgacgcgctg
     2461 gagtggaagg ggaaacccag gcgccagctc gatgctgtgc tggatgaaat aggctatgcg
     2521 ccctgtgccc gggaggccgt gtcggcagcg cgggaggcgg gcgcctcggt cgctctcgtc
     2581 tcgtccgggc tgtcgttcct ggcctctcga gtcgcacgtg agctgggtat ttgccatgtt
     2641 ttcgccaatg agctagcagt atgcgacggc cttatgacgg gagaagtggt gataaacgtc
     2701 tccatagacg atcccgccag gacgaaggcc gcaatactca aacaactcag gagtgcactt
     2761 agggccgata gggccgagac ctgggctgtc ggcgacaact ggggcgatat cgagatgctg
     2821 caggaggcgg gtcgtgcctt cctggtagcc ccccggcccg agtgctggga gcaggctatg
     2881 caggccgccc ctcatgtaga gtgcatcgcg gacctatgcc atctggtcag cctcatccga
     2941 aacgtaagcg tctgcgcgga ttgacggggt gggcagccca aagtcatctt tgtactgcag
     3001 tctgtacaag tcgtagtaga tccccttgtg cgccaacagc tcctggtggg tgcccatctc
     3061 tcggatacgc cctttgtgaa gcacgattat cttgtcggca tgctggatag tggacaaccg
     3121 gtgagccaca acgattgtcg tgcgtccggc gatcagcctt ggcagcgcat cctgtatcag
     3181 gagctccgtc tcggtgtcga tattcgccgt ggcttcgtca agcacgagta tcgccggatc
     3241 gaaggccagg gcgcgtgcga acgcgagcaa ctggcgctgg ccggcagaaa gcgtagaacc
     3301 gcgctcagtc acgggttcat cgtaaccgtg gggcaaccgt gagatgaaat ggtccgcgtt
     3361 tacgtaatgg gctacctctc ggatcttctc atccgatatg tcggtgttgt tcagtcggat
     3421 attgcccttt atgtcgccgg taaagaggaa tacttcctgc aacaccgtgg cgatgttctt
     3481 cctgagatcg cttactcggt accgccggat gtcgatcccg tcaaccagta tcgcgccctt
     3541 ctgaatgtcg tagaacctgt tcatgaggct gataactgag gtcttgccgg ctccggtggc
     3601 tcccacaaag gcgaccatct cgcccggctt tatggtgaag ctaacatcgc gaagcaccca
     3661 gtcgtcgttg ctataggcga accatacgtt cttgaactcg atatcgcctc gtacctggcc
     3721 gatgtcgatt ggatcggccg gatcgcgaac ggtgataggc gtgtcaagca gctggaacat
     3781 gcgctcggat gaagccatgg cggcctgaag gatattgtac ttctcagtga ggtcgttgat
     3841 ggggccaaag aacattccaa tgtagttgat aaatgcatat accaggccga atggcatctg
     3901 tcccgtgatc acgcgtctgc cgccgtacca tacgaggagc gatagcgcca gagtgcctat
     3961 gagctccact atgggccgga aaacggcgta gaccatcatc tcaccgatac ccgccttgta
     4021 gtaatcgcgg ttcacctcgg cgaactcgtc ggacttcttc ttctcacggt tgtagatctg
     4081 cacaacgcgc atgcctgaga tgctctcggc cataaatgcg ttgatccttg caagtgcggc
     4141 cctgactcgc ctgtaggcgt cacgcgcctt gactctaaat acgacagtga ccagcgcaat
     4201 caggggcaat gtggccatgg atactagagc gagctgcgtg ttgaggcgga acatgactgc
     4261 aatgatgcct atgagcatga acacgtcctt gaacagattc actaggacgc tggtgtacat
     4321 ctcattgagc gtttcagtgt cgttggtcag gcgcgttaca agacggccta cggggctgcg
     4381 gtcgaagaaa gccaggtcca agtggcttat gtgagtgaac acttcctgcc gtatgttgta
     4441 gactatgcgt tggccaacgt agtgcagcgt gacggtttgg ccatagttga gcgcgaaggc
     4501 gcccatcccg acaactagca gcaacagggc aagccttccg atggcttcga tatcgcgccc
     4561 gcggaactcc cgaacggcat cggcgctatc cactatctcg gcagcgtagt ccagcgaatc
     4621 tacgtggaga accgcccctt ctgcagtttc aaccagctcg gcctgaccgt gctccggagg
     4681 caggacctta tcgattatca cgtacttgcc cgccacggct agcatctggt agtgagtttc
     4741 atggcgcatc tcgacaggga gatgctcgga ccttacaaat acggacccag cgtagtgcat
     4801 tctgtgctgt tcacgtacgt cggcaggtac ctggtccggc tcaaagcggg ccatggggtt
     4861 ctcgtagccg gtcacgtatt catcgatggc gatcttgata acatacggcc ttaccaggtc
     4921 tgctgcagca atcaccatca agaccacgag cgctgcggca aagatggccc agtatggcct
     4981 ggcatatcta agcaggcggc gcatgagctt ggcatcgtac gcctttccca ctatctcctc
     5041 ttcgttgctg tatccgttca ttgcagatcc acctcttcag ccagcctgcc agccagctag
     5101 ctcttgctgg ccaattcctc ctcgaggagc tgcttctcat gtagctcgta gtagagcccg
     5161 ccagcagcta ccagcgtgtc gtgagtacct cgctcccgaa tgacgccatc gtcaagaacg
     5221 atgatctcat ctgcgtcctt cactgttgaa atgcggtgcg agattatgat gctggtacgg
     5281 ccgcgcatgt actcgcgcag tccgcgaaga atgctctctt cagtctgcgt gtctacagcc
     5341 gacaggcagt catcgagcac gagcaccttc ggctccttga tcagagcacg cgcgatgcat
     5401 atgcgctgtt tctgacctcc cgacagagtc acaccgcgct cgcctaccat tgtgtcgaat
     5461 ccgtcgggga attcggaaat gtccttacgc acctgcgcta cctcggcgaa atgctcgatt
     5521 cgctccagcg gatattcatc agacgcgaat gcgatgttct tgccgatggt cgtcgagaac
     5581 aggaatgact cctgcggaac cactcccagc tgagtgcgga gggattcaag ggtgacctgc
     5641 ctgatgtcgt gtccgtcgat gaacacgctt cctggcgggg gattgtacag acgggccaga
     5701 agactgacta tcgtactctt tccgctgccg gtcctgccca caacggccag cgtcttgcct
     5761 gcctcaatag taaatgatac gtcgctcagc gcaggcggca gttctggcga gtacgagaac
     5821 gttacgtttc ggaattcgat tgtgccggcg accgagggga gctcaactgc ccctggttca
     5881 tcggcaactt cgggcgcctc attcaagatc ttgttgattc tgtctagagc ggcgtagcca
     5941 cgctgaacca cgtttatcac ccagccgact gcgatcatcg gccacactag cattgccaga
     6001 tagctggtga gggccacgaa gtctcccagc gatatctcct gcctgattat catggccgat
     6061 ccatacttga gtacgaatac gaaggccaga gtcgacgcaa ggccgctcat ggggaacatg
     6121 agaccccata tgcgtatcag tcgcatgttc tcgtccacgt tcttctgggc gacatccatg
     6181 aacctgcttg tttccgcttt ctcctgcacg aacgccttca ccactcggat gcctgctata
     6241 ctctcctgtg ctcgctcggt gagatcggag aaggcctcgt gcacggctcg gaaacgcggg
     6301 ccga
//
LOCUS       DAOTAC010000143         6302 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_10472_length_6302_cov_4.939331, whole genome shotgun sequence.
ACCESSION   DAOTAC010000143 DAOTAC010000000
VERSION     DAOTAC010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6302
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10472_length_6302_cov_4.939331"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="PLM74_07135"
     CDS             complement(<1..260)
                     /locus_tag="PLM74_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004273136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HON42883.1"
                     /translation="MKACDSSSVLARAIAEQEHMVTARRTIHHRPELGFEEHATARLI
                     ADRLQALGLEVRTGVGGTGVLAVLRGYEPGPTVALRADMDALP"
     gene            complement(301..1719)
                     /locus_tag="PLM74_07140"
     CDS             complement(301..1719)
                     /locus_tag="PLM74_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HON42884.1"
                     /translation="MGRYTAVTSRIVDELKRILDPARVVTDPEKLAPYAHDEVSEAAY
                     VRMPDVVVKPNTTAEVSDILQLANRENIPVTPRGAGTGLSAGCVPMYGGIVLSLENMN
                     RVIEVDTRNLFMVVEPGVTTGEVQKIAREQGLLYAGDPCSSESSQIGGNLAENAGGNK
                     AVKYGTTSKHVYGLEVVMPTGEIVTTGGKCVKDVTGYDLVHLFVGSEGTLGVITKAWL
                     KLLPLPKFRVDLLAPFDDMQKAIEVVPRVMTESGIVPTAVEFMDSLSIRAAEMYLNSS
                     LPHSDAAAYLIIELEGSSEEQVEAEYEAIGNLCIESGALEVYVADNLATQDRIWKARK
                     CVAEALRVVSPVYCMEDITVPTSEIPELLWSISQISKELDVKIPAFGHAGDGNIHATL
                     LREGMDDATWETVKEKALHEMYTATYQHGGNLSGEHGIGAKRLVPLERFVDRAQIQVI
                     KSIKAALDPNLILNPGKIVHMD"
     gene            complement(1726..2745)
                     /locus_tag="PLM74_07145"
     CDS             complement(1726..2745)
                     /locus_tag="PLM74_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HON42885.1"
                     /translation="MEYSGVLVVGEQRGGSVHSVTYELLARGRTLADKLGVGLACVVI
                     GHELEAFGREIVERGADIVYLIDDPCLEWFIPRRYSAELAALIEQIRPEIVLAAATTA
                     GRTLMPHVAAKLDTGLTADCTGLDIDDKERLLLQTRPAIGGNVMATIKTPSARPQMAT
                     VRPKSARPLPRDLGRQGEIVVRAAACDDAQCAGECLLGFVRDTSQDVSVQDAEIVVAG
                     GKGLKSRDNFRMVEELAGLLGAAVGATRDVVDLGWIGYPHQIGLSGKTVSPRLYVAIG
                     LSGTIQHLAGMQTSETIVAINKDKDAQIFRVADYGIVGDLFEVVPELIDKIRAAKNAR
                     LCGAR"
     gene            complement(2776..3579)
                     /locus_tag="PLM74_07150"
     CDS             complement(2776..3579)
                     /locus_tag="PLM74_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007933621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HON42886.1"
                     /translation="MNLFVLLKQVPGTSNVKMDEKTGTMVRTDHENVINPLDENALEA
                     ALELKRSIDGSKVTVVSMGPPPASRALKEAIAMGADEAHLLCHRGFAGSDTLATARAL
                     ALAIEHVAGRELGPSDLVLCGERATDGETGQVGPMISAMLDIAVATYVRGIEVKEDHV
                     VVERVVEEGFERVRLPLPALVTVVKDINQPGFPTLAGKLAAKAAPVPLLTPDDLGLTR
                     DTVGLSGSPTRVVQVFRPKFARDTVLRQQDSGGAAVDSLVDLLIEKEVI"
     gene            complement(3613..4413)
                     /locus_tag="PLM74_07155"
     CDS             complement(3613..4413)
                     /locus_tag="PLM74_07155"
                     /inference="COORDINATES: protein motif:HMM:NF013916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42887.1"
                     /translation="MNTGVQIRMSRLFNAATGKSVIVAVDHGIEGVPRGLEDPRQKVQ
                     ALIESGVDALLLNPGLFERVSGLWERRNAPGVVLAADYYMGSTVPGRKAQTEDYRRIL
                     EVEDAVRLGADAIKMVLVFGQRELDLYAYNLELVAASIQEAHRLGMPVMLETVLWGIT
                     STQEEMASVEVLRHMMRIAIELGADILKAPLVGTDDEFAEVVRWSPVPVTVLGGQTRP
                     EFDSVVEGARRSANLGARGIVFGRNVWQRPDLAAAVHALQTAVHGCEH"
     gene            complement(4452..5441)
                     /locus_tag="PLM74_07160"
     CDS             complement(4452..5441)
                     /locus_tag="PLM74_07160"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HON42888.1"
                     /translation="MSLPSTMKEVKLFGLGDLRIVESPVPTIEPDEILVRVRACGICP
                     TEIRKFTVPNYKPIPFPVNPGHEWTGDVVAVGPKVKRFQVGWRIVAEGEGGYAEYAKV
                     TASNLQYSCRLPDSVSYEEGTFVEPLSDCIHAVRERANVIAGDRVVVVGAGPMGLGIV
                     AAAARSGANVMAVEPIPERRALALEFGAETVIDPNAESVEEAVLEWTGGRRANVCMAT
                     VGISKVMESCMKLVGERGRVVLFGGGGAGQTITIDPNWIHYNEISIVGSEWIGVGGKE
                     EKDLYRISTEWIADGKVPVNRLISHRFPLTKIHDAYETIQAGGTLKVILLTDR"
     gene            complement(5478..>6302)
                     /locus_tag="PLM74_07165"
     CDS             complement(5478..>6302)
                     /locus_tag="PLM74_07165"
                     /inference="COORDINATES: protein motif:HMM:NF014801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HON42889.1"
                     /translation="VVSREAASETALPEGTPVIAGTGDAADEMVGTGVIDEGSLALLY
                     GSTLATMSPVSRAWLHPGFILTPGLAPGSYLVSSVLGTGSALIEWIRGTLAWDGSMPD
                     HATLEGEAVGVPAGSNGLVFIPYLTGQRSPEVRPNMSGALLGLNQGHSLGHVYRAALE
                     GVAFALRSCLAELSSAGLLGEYEIRAAGGGSQSELWTQIVTDVCRHEQRITSGSVRAP
                     IGSAFLAGRGVGIVDACDLRETWVGFRSTVSVCESCADEYDHHYQRFMRYVSLLAT"
BASE COUNT         1253 a         2087 c         1610 g         1352 t
ORIGIN      
        1 ggaagagcgt ccatgtcggc tcgtagcgct accgtggggc caggttcata gcccctcagc
       61 actgccagca cgccggttcc gcccaccccg gttctgacct cgagcccgag ggcctgaagt
      121 ctatcggcta tgagccgggc ggtggcgtgc tcttcaaacc ccagttcagg ccggtggtga
      181 atggtcctcc tcgccgtaac catgtgctct tgctcggcaa tggctctggc aagaactgag
      241 ctgctatcac atgccttcat gtggcttcat cctcaattct tgatgcgtgc tgcatgtagt
      301 tcagtccata tgcacaatct tcccggggtt taggatcagg tttggatcca gtgccgcctt
      361 aatagacttg atcacctgta tctgtgcccg gtccacgaag cgctccagcg gcacgaggcg
      421 cttggcgcct atcccgtgct cgcccgacag gttcccgcca tgttggtagg tggcagtgta
      481 catctcatga agcgccttct ctttgacggt ctcccaagta gcatcatcca tcccctccct
      541 aaggagagtg gcatggatgt ttccatcccc tgcatgccca aaagccggga tcttgacgtc
      601 tagctccttc gatatctgcg atatgctcca cagaagttca ggtatctccg aggtcggaac
      661 ggtgatgtcc tccatacagt agacgggact caccacgcga agggcttctg caacgcactt
      721 gcgtgccttc catatccggt cctgggtggc caggttgtcg gcaacgtata cctcgagagc
      781 tccagactct atgcacagat tgccaattgc ctcatactcg gcctcaacct gctcctcgct
      841 gctaccttcc agttcgatta tgagataggc ggcagcatca ctgtgcggca aagacgagtt
      901 gaggtacatt tcggcagctc taatagacag gctatccatg aactccactg ccgttgggac
      961 aatgccgctc tcagtcatca cacgcggcac cacctcaatc gccttctgca tatcatcaaa
     1021 gggagccaaa aggtccaccc ggaacttggg cagcggcagc agtttgagcc atgcttttgt
     1081 gatcacgccg agcgttcctt cggaccccac gaacagatgc accaggtcat agccggtaac
     1141 gtccttgaca cacttgccgc cggtcgttac gatctcgccg gtgggcatca ccacttccag
     1201 cccatagaca tgctttgaag tggtgccgta tttcaccgcc ttgttgccgc cggcgttctc
     1261 cgcaaggttt ccgcctatct gagaactctc tgagctgcat gggtctccgg catacagcaa
     1321 cccctgctcc cgcgcgattt tctgaacctc tcctgtagtc acaccgggct cgacgaccat
     1381 gaacaggttg cgcgtatcta cctcgatcac ccggttcatg ttctccaaag acagaactat
     1441 gccgccgtac atcggcacac agcccgccga tagccccgtg cccgctcctc gcggggtgac
     1501 cggtatgttc tctctgttgg ccaattgcag tatgtcagag acctctgccg tcgtattcgg
     1561 tttgaccacg acatcgggca tacgcacgta tgccgcttcc gacacctcat cgtgggcata
     1621 cggagcgagc ttttcggggt cggtgaccac acgggcggga tccaagatcc gtttcagctc
     1681 gtcaactatc ctgcttgtca cagctgtata tcttcccatc gtcactcacc tcgctccgca
     1741 aagacgcgca ttctttgccg cccggatctt atcgatcagc tcagggacta cctcgaagag
     1801 gtcgcccact atgccatagt ccgcaaccct gaagatctga gcgtctttgt ctttgttgat
     1861 tgctacgatg gtctctgatg tttgcatgcc cgcaagatgt tgtatggtgc cagacagtcc
     1921 tatcgcaaca tacagacgcg gcgaaactgt cttgccgctc agccctatct ggtgcgggta
     1981 cccgatccat cccaggtcaa ccacatcccg cgtcgcgccg actgctgcgc caagcaaccc
     2041 ggccagctcc tctaccatcc tgaagttgtc acggctcttc aggcccttgc cacccgctac
     2101 aacgatctcc gcatcctgca cgcttacatc ctgcgacgta tcgcgcacga acccgagcaa
     2161 gcactcgccc gcgcactgcg cgtcatcgca tgcagccgcc ctgacaacga tctcgccttg
     2221 ccgcccaaga tcccgtggaa gaggtcgggc cgacttaggc ctcactgtcg ccatctgagg
     2281 cctagccgac ggcgtcttga ttgttgccat cacatttccg cctatggccg gcctggtctg
     2341 cagaagcagt ctctctttat cgtctatgtc aagcccagtg caatccgcgg tcagccctgt
     2401 atcgagcttc gcggcgacat gcggcatcag agtcctgccg gcagtcgtgg cggcagccaa
     2461 aacgatctcc ggccgaatct gctcgatcaa agccgccagt tccgctgaat agcgcctagg
     2521 aatgaaccac tccaggcaag gatcgtctat caaatagaca atgtccgcgc cccgttccac
     2581 gatctcacgt ccgaacgcct cgagctcgtg gccgatcacc acgcacgcaa ggcccactcc
     2641 cagcttgtca gcgagcgtcc ggcccctggc cagaagctcg tatgtgacgg agtgcactga
     2701 tccgcctctt tgctcaccta cgactaacac gcctgagtat tccaccgatt caccctctct
     2761 cgggaaacgc acgtactaga tgacttcctt ctctatcaac agatcgacga gcgagtccac
     2821 agcggcgccg cccgagtcct gctgcctaag gacggtgtcg cgcgcgaact taggcctgaa
     2881 cacctgcacc acgcgagttg gcgatcctga aagcccaacc gtatcgcgag tcagccccag
     2941 atcgtcaggg gtcaagagcg gcaccggcgc cgccttcgca gccagctttc cggcgagagt
     3001 aggaaagccc ggttggttga tatccttcac taccgttaca agtgcaggca gaggcaatcg
     3061 aacgcgctcg aagccctctt caaccactcg ctcaaccacc acatggtctt ccttgacctc
     3121 aattcctctg acgtaggtag cgactgcaat gtctagcata gcggagatca tggggccaac
     3181 ctgcccagtc tcaccgtcag tcgcccgttc tccgcagaga acaagatcgc tcgggcccaa
     3241 ttccctgcct gccacgtgct ctattgccag tgccagcgcc cgcgctgtgg ccagggtgtc
     3301 ggagccggcg aatccccggt gacatagcag atgcgcctca tccgccccca tggctatcgc
     3361 ctctttgagg gcccgggacg ccggaggcgg ccccatgctc acaaccgtaa cctttgagcc
     3421 atcgatcgac ctcttcagct caagtgcagc ctcaagcgca ttctcgtcca acgggttaat
     3481 gacgttctca tgatcggtcc tcaccatagt cccggtcttt tcgtccatct tgacattgga
     3541 ggtgccgggt acctgcttga gcagtacgaa tagattcatg ttgaccacgc gcctcttcag
     3601 tgttctccgt cgtcagtgtt cacagccgtg gactgcagtc tgcagagcat gcaccgcggc
     3661 cgcaagatcc ggacgctgcc agacgttcct tccgaagact ataccgcgcg cgcccagatt
     3721 cgctgagcgg cgggcgcctt ccactacact gtcaaactcg ggtcgagtct gccctccaag
     3781 gactgttacc ggcacaggcg accaacgcac gacttccgcg aactcatcat cagtgcccac
     3841 aagcggcgcc ttgaggatgt cggcaccaag ttcaatggca atacgcatca tatgacgcag
     3901 tacttcaacg ctggccattt cctcctgggt agaggtgatg ccccacagaa ccgtctcgag
     3961 cataaccggc atgccgagcc tgtgcgcctc ctgtattgaa gctgcaacta attcgaggtt
     4021 gtacgcgtac agatccagtt ctcgctggcc gaataccagc accatcttga tggcgtccgc
     4081 cccgaggcgc acagcgtctt ctacctctag gattcgccga taatcctctg tctgggcctt
     4141 ccgtccaggc acagtgctgc ccatgtagta gtcggcggcc agcacgaccc ctggagcgtt
     4201 tcggcgctcc caaagccctg atacgcgttc gaacagcccc gggttgagta ggagtgcatc
     4261 tactcccgat tcgatcagtg cctgaacctt ctgccgcgga tcctcaaggc cacgcggcac
     4321 gccctcaatt ccatgatcga ctgcaacgat aactgacttg cctgtagcgg cattgaacaa
     4381 cctgctcatg cggatttgaa ctccggtgtt catccgccta cgccccctct ccccatgtga
     4441 atctacagca tctacctgtc ggttagcaga atgaccttca aggtgccgcc ggcctgaatg
     4501 gtttcgtaag catcatgtat cttcgtgagc ggaaaacgat gcgagatgag gcggttaacc
     4561 ggcaccttgc cgtctgctat ccactcggtg gatatgcggt acaggtcctt ctcttccttg
     4621 ccgcctaccc caatccactc gcttccgact atggatatct cattgtagtg gatccagttg
     4681 gggtctatgg tgatggtctg ccctgccccg cctccgccga agagcaccac acgaccgcgt
     4741 tcgccgacaa gcttcatgca cgattccatg accttgctga tcccgacggt ggccatgcag
     4801 acattggccc tgcgaccgcc ggtccactcg aggacagctt cttccactga ctccgcattc
     4861 ggatcaatta ctgtttcagc gccgaactcc agggccaagg cgcgtctttc ggggattggt
     4921 tcgacagcca ttacgtttgc accggagcga gcagctgctg ccacaattcc gagccccatc
     4981 gggccggcac ccacgaccac aactcgatcg cctgctatga cgttcgcgcg ctccctgacg
     5041 gcgtgtatgc aatcggagag gggttccacg aacgtaccct cttcgtagct aacactatcg
     5101 ggcaggcggc aggaatactg caagtttgac gcagtaacct ttgcatactc agcgtatccg
     5161 ccctctccct cggcaactat gcgccaacct acctgaaagc gcttcacctt cggccccacg
     5221 gccaccacgt cgcctgtcca ttcatgcccg gggttcacgg ggaaggggat cggcttgtag
     5281 ttgggcacag tgaacttgcg tatctctgta gggcatatcc cgcaggcgcg cacccggacc
     5341 agaatctcat caggttctat ggtcgggacg ggagactcga ctatgcgaag gtccccgagt
     5401 ccgaacaact tgacttcctt catcgttgac ggcaaactca cgtccagaac accctcctaa
     5461 cagcatctgt tgttgcatca cgtagcaagc aggcttacat atctcatgaa gcgttgatag
     5521 tgatgatcgt actcgtcagc gcatgactca cacacgctca cagtgctccg aaaaccgacc
     5581 catgtctcac gcagatcgca agcgtcaaca atgcccacgc cacgtcctgc caaaaacgct
     5641 gagcctatgg gcgctctcac cgagcctgaa gttatcctct gctcgtgccg gcacacatct
     5701 gtgacgatct gtgtccatag ctcgctttgg cttccgcctc ccgcggccct tatctcgtat
     5761 tcccccaaaa gccctgcgct tgatagctcc gccaagcatg agcggagtgc gaatgccacc
     5821 ccttcaaggg ccgcgcgata cacgtgcccc agcgagtggc cttgattgag cccgagcaag
     5881 gcccctgaca tgttcggacg aacctcaggg cttcgctgtc cggtcagata tgggatgaac
     5941 accagcccat tgctgcccgc aggcacccca acggcctcgc cctccaatgt agcgtggtcg
     6001 ggcatggatc catcccacgc cagcgttccg cgaatccatt cgattaaggc ggatcctgtt
     6061 ccgagcacac tgctcaccag gtagctacct ggcgcaaggc ccggagtgag aatgaaacca
     6121 gggtgaagcc acgcccggga cacaggagac atagttgcca aagtggagcc gtagagcagg
     6181 gcgagcgatc cctcgtcgat aacgcctgtg cccaccattt cgtctgcggc gtcgcctgtg
     6241 cccgcaatga ccggcgtgcc ttccggcagg gccgtctcgg aggccgcctc gcgactcaca
     6301 ac
//
LOCUS       DAOTAC010000144         6270 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_10543_length_6270_cov_4.241352, whole genome shotgun sequence.
ACCESSION   DAOTAC010000144 DAOTAC010000000
VERSION     DAOTAC010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6270
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10543_length_6270_cov_4.241352"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="PLM74_07170"
     CDS             complement(<1..391)
                     /locus_tag="PLM74_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004083260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HON42890.1"
                     /translation="MKAVVLHADWQPRPDFKLGSKDIDGKLTYLGSRVWRNPRIQVEE
                     KPIPRPGPTEALIRVRAVGICGSDVHMAQADEDGYIFYPGLTAFPATLGHEFAGEVVE
                     AGQRAINKRTRKPYEPGEAVCSEEMLWC"
     gene            complement(412..1443)
                     /locus_tag="PLM74_07175"
     CDS             complement(412..1443)
                     /locus_tag="PLM74_07175"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HON42891.1"
                     /translation="MEPTIRDVARLAGVSKSTVSRVLNNDPNVSDHAKGAVGDAMREL
                     NYRPNALARGLCTSRTGTIGLIMSDITNPFHAEVARGVEDLAADYESNVILCNTDGRP
                     TKEQAYIDLLLEKRVDGVIFLSAKMGESDVSMLRARRVPFVFAGRTLLDVEADSVVVD
                     SVLGGFQATNHLIGLGHRRIAHISGPARVSASADRYEGYCQALRRAGISIDPDLVADG
                     DFRQDRGYRAMSQLLDGKTGMTAVFAGSDYMALGALEAIYERGLRAPDDIAVVGFDDI
                     SFARLHLVQLTTVAQPKYDIGAIAARMLLERIGDLSADRPPQKVVLPPKLVIRRTCGD
                     YLGPRDFHS"
     gene            1759..3291
                     /locus_tag="PLM74_07180"
     CDS             1759..3291
                     /locus_tag="PLM74_07180"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HON42892.1"
                     /translation="METADQPGVMRVCTGVTPADVLMGAGLPALKAHHIVSTLAIQSV
                     AYLEEAVRRGSVKALLGGTTNMDDHVLRFIRLGKRIAPSTQITDQTRAGVPLAYAAWV
                     GRMVVSALRAMPGVLSAVTAGQVRRQCEVVDSLDLVVSTENPADLARAPVGLPVVVSA
                     AATPDVLHADVLGMDIRIHVTTPRSLASRLLDATGSACHVHRLAELAAERGLFLDRGV
                     LVHSITGRQVLLEDEAEIYAQLGLPFIPPEYRVGSDEVEEALAGTLPCPVGHEDIQGD
                     LHLHTDWSDGLDTVEDMVAEAMRRGYSYVAVSDHTAYHSTAHGLSAKRLLAQWDAIEA
                     LQRRCPSIAILKSAEVDILPDGTLDMSDDVLHKADIVLASVHKHLDMEPRALTRRLIR
                     AMENPHVDVLAHPTGRSIGLSDYRVDVDELTCAAARLKVGLEINCAPDRLDIDERLAR
                     RCNDLGIALAIGSDAHSVGEMDQFGLGVGVARRARCTSHSILNTRSPQELRAWLDWHR
                     ND"
     gene            3389..4393
                     /locus_tag="PLM74_07185"
     CDS             3389..4393
                     /locus_tag="PLM74_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013932392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HON42893.1"
                     /translation="MTRQMVRAFALLALLLILSAATVAAPARVLHLLTALDPNEAKIY
                     IAAFEKSTGVKIEWVRMSAGECLARLRAESRNPQFSVWFGGPSAEYVPAGQAGVLESF
                     KPEGSDFLSPQQRDSNWNWVGFYFGALGFACNTDWFAKNNIAFPSSWQDLLKPQLKGQ
                     ISFAYPYTSGTAYTLLATMVQLLGEEKGMDYMKRLDANVHHYNTSGSACVTQAGLGEI
                     AVGIAFSHDVMAKGTAKGYPVKLTLPSEGTGYEIGAMAVVKGGPEPDLAKQFITWALS
                     TECQDLMQEWFRIPLNPRAKVAPGAVGPDQVKLIPYDDVWAGQNQARLIDKWREMTNK
                     "
     gene            4522..6225
                     /locus_tag="PLM74_07190"
     CDS             4522..6225
                     /locus_tag="PLM74_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HON42894.1"
                     /translation="MIVREPAVAITIMVIASALAVFVVYPLYMVLRTSLTTDGGLSLA
                     IFRSLLSRSYNRRPLANSLKLGLVVSAMGTAAGFIFAYSTTRVDVPLRGFFRLVATFP
                     MISPPLIMSLATILLFGNNGLITRQILRGAVDFGIYGFRGLALVETLAYFPTAYLVLS
                     GVLQAMDPSIEDAALDLGASWWQMFRTVTLPLAIPGLASALLLIFIESLADFGNPIIL
                     AGNYTVLATQAYLRITGLYDLSGGAALAVILLIPSLIAFFLQKYWVGRRSYITVTGKP
                     SAARTRRDEPWLRWCAFTLCLILTGMILLLYGTVFIGSFVTLWGANYELTLANYNHVF
                     RVGWNHIRDTIFLSTVSTPITGILGMVIAFLVVRKQFPGRRALEVASMLTFAIPGTVV
                     GIGYILAFNQPPLVLTGTAAIIIILFIFRNMPTGIQSGIAALQQIDPSIEEASYDLGA
                     DSATTFRKITLPLIAPAFFSGLVYSFVKCVTAISAIVFVVSARWKLITVAVMGSVENS
                     DLAQAAAFCVVIIAIILAAIGIINLLVYPPWRARSTARAATVGTSGAPGAIPPGVAGG
                     Q"
BASE COUNT         1292 a         1854 c         1792 g         1332 t
ORIGIN      
        1 cgcaccatag catctcctct gagcagaccg cttcgccagg ttcatacggc ttgcgtgtgc
       61 gtttgttgat ggctctttgg cccgcctcca cgacctctcc cgcaaactcg tggccaagcg
      121 tcgccgggaa tgcagtgagg cccgggtaga agatgtagcc gtcctcatcg gcctgtgcca
      181 tgtggacatc gctgccgcag atgcccacgg cccttacccg aatgagcgcc tcagtggggc
      241 cgggcctggg aatgggtttc tcttcaacct gaattcttgg gttacgccac actctactgc
      301 cgaggtaggt cagcttgccg tcgatgtctt tcgaccccag cttgaaatcg ggcctaggct
      361 gccagtccgc atggagcact accgctttca ctgagagtcc cccctatgca tttatgaatg
      421 gaaatcgcga ggtcctaggt agtcgccaca ggtacgccgt attaccagtt tcggaggaag
      481 caccactttt tgcggagggc ggtcggccga taggtctcca atccgctcta ggagcatccg
      541 cgccgcgatc gccccgatat cgtacttggg ctgagccacg gttgtgagct gaaccaagtg
      601 caggcgggcg aaggagatgt cgtcgaagcc cacgaccgcg atatcgtcgg gcgccctgag
      661 accgcgctcg tatatggcct cgagagcccc cagagccata tagtcgctcc ctgcaaacac
      721 agccgtcatg cctgtcttcc cgtcgagcag ttggctcatc gctctgtacc cgcggtcttg
      781 cctgaagtcg ccgtccgcga caagatcagg gtcaatggag attccagccc gccgaagcgc
      841 ctgacaatag ccttcgtacc ggtcggcgct ggcggagacg cgcgccgggc ccgaaatgtg
      901 ggctattcgc ctatggccca gaccaatgag gtggttcgtg gcctgaaagc cgccgagaac
      961 gctatctacg acaacactgt cggcctccac gtccaaaagg gtgcggccgg cgaacacaaa
     1021 cggcacccgc ctggcccgga gcatcgacac atcactctcg cccatcttgg ccgagaggaa
     1081 tataaccccg tccactctct tctccaacag caagtctatg taggcttgtt cctttgtagg
     1141 cctaccgtca gtgttacaca gtattacatt gctctcgtaa tcagcagcca gatcctccac
     1201 gcctcttgcc acttcagcgt ggaacggatt cgtgatgtcc gacataatca ggccgatagt
     1261 ccccgttctg ctcgtacaca gcccgcgtgc cagcgcgttg ggcctgtagt tgagctccct
     1321 catggcatcg cccacagcgc cctttgcgtg atcgcttacg ttgggatcgt tgtttagcac
     1381 gcgcgagaca gtggacttcg atacaccggc cagcctcgcc acatcgcgga ttgtcggttc
     1441 caccggaatc ctccttgatc gaccacgcgg tgaagtagca taagtgtgtt cccaggcgcg
     1501 tgggaccggt cccagaactg tagttcaact cctccacgtt aactccttct tatcccattt
     1561 ttatatgaat ttgactattt gccgtgtaat gtacagtacc gtagcaaagt aggccgtttt
     1621 ccataactca gtccagaagc gccttctaga tccgtgtatt ccatctgctt ttttagcaag
     1681 gattcccatg catagcgcct aatcgtaccg ccagtgcatt tgccatacac catggcgccg
     1741 aaggagtcat gacacgtcat ggaaaccgca gatcaaccag gtgtcatgcg agtttgcacc
     1801 ggtgtaactc cagccgacgt cctcatgggc gccggtttgc cagcacttaa ggcacaccat
     1861 atcgtctcaa cgcttgcgat ccagtcggtg gcctatctgg aggaagctgt aaggcgcggc
     1921 tcggtgaaag ccctcctagg cggaacgacc aacatggacg atcacgtgct aaggttcatc
     1981 cgtctcggga agcgaatagc accatctacg cagatcactg atcagaccag ggctggagtg
     2041 ccactggcgt acgccgcctg ggtgggacgt atggttgtct cagccctgcg ggccatgccc
     2101 ggcgtgttgt ctgccgtcac ggccggacaa gttcgaagac agtgcgaagt agtcgacagc
     2161 ctggacctgg ttgtatcaac tgagaatccg gcggatctcg cccgggcgcc cgtcggcctg
     2221 ccagtagtcg taagcgcagc tgctaccccg gacgtcctgc atgccgacgt gctaggtatg
     2281 gatatcagga ttcacgtgac tacgcctcgg agtttggcga gtcgtcttct cgacgccaca
     2341 ggctccgcct gccatgtcca tcgtctggcc gagcttgcag ccgagagagg gttgttcctc
     2401 gaccgcggag tgctagtcca ctccattacg ggtcggcagg ttctactgga agacgaggct
     2461 gagatctacg ctcagcttgg tctgccgttc atccctcctg aatacagggt aggaagcgac
     2521 gaggtcgagg aagcactcgc cggcacactt ccttgcccgg tggggcacga ggacatacag
     2581 ggagatctgc acctgcacac tgactggagt gacgggcttg atactgtcga ggacatggtt
     2641 gcagaggcca tgcgcagggg ctacagctat gtcgctgttt cggatcatac agcgtaccac
     2701 agcacagccc atgggctttc tgccaagcga ctgctagccc aatgggacgc catcgaggca
     2761 ctgcaaagac gctgtccttc cattgccata ctgaagagcg cagaggttga catcctgccc
     2821 gatgggacac tcgacatgag cgacgacgta ctgcacaagg ccgatatcgt tctggcctcg
     2881 gtacacaagc acttggacat ggagccgcgc gcgctaaccc gtcgcctgat tcgggcgatg
     2941 gagaacccac acgtcgatgt gttagcgcat cctaccggca ggagcatcgg gctatccgat
     3001 tatcgggtgg atgtagatga gctgacgtgt gcagccgcga ggctgaaagt cggccttgag
     3061 atcaactgcg cgccggaccg cctcgacatc gacgagaggc tagcgaggcg ctgtaatgac
     3121 ctgggcatcg cgcttgccat aggcagcgat gcccacagcg tgggcgaaat ggaccagttt
     3181 ggcctgggag taggagtcgc tagacgggcc agatgcacaa gccatagcat actcaatacc
     3241 agatcgcccc aagagcttcg ggcctggctg gattggcatc gaaacgattg agactggagg
     3301 tggtcgttgg tacggatcat gatcttcgaa cccagtacag agtcggcact gcattcagca
     3361 ttgataacac acaagggggt cgaatcccat gacacggcag atggtgcgag cgttcgccct
     3421 tctagcactc ttgctcatac tgtcggctgc cactgttgct gccccggcaa gggtcctgca
     3481 cttgttgaca gcgctggatc caaatgaggc gaagatctac atagcagcct tcgaaaaatc
     3541 cacaggggtc aaaatcgaat gggtgaggat gtccgctgga gagtgcctcg caaggctcag
     3601 ggcggaatcc agaaatccgc agttcagtgt gtggttcggc gggcccagcg ccgagtacgt
     3661 tccggcgggt caggccggtg ttcttgaatc cttcaagccc gagggcagtg acttcctcag
     3721 ccctcagcaa agggattcca actggaactg ggtcggcttc tacttcggcg cgctcggttt
     3781 cgcgtgcaac acagattggt tcgcaaagaa caacatcgcc ttcccctcgt cttggcaaga
     3841 cctgttgaaa cctcagctca aggggcagat ttcgtttgcc tatccataca cgtccggcac
     3901 cgcctacacc ctgctggcca cgatggtgca gttgctgggt gaggagaaag gcatggacta
     3961 catgaagaga ctggacgcaa acgtacacca ctacaacaca tccgggtccg cgtgcgtgac
     4021 ccaggcaggg ttgggcgaga ttgcagtggg catcgccttc tcccatgacg tcatggccaa
     4081 aggaacagct aaaggatacc cggtgaagct gacccttccg tctgagggga ctgggtacga
     4141 gattggagcc atggctgttg tgaagggcgg gcctgagcct gaccttgcaa agcaattcat
     4201 cacctgggcg ctgtcaaccg aatgccagga cctcatgcag gaatggttcc gaattccgct
     4261 caaccccagg gccaaggtgg cgccgggcgc cgtcggtccg gaccaagtca agctaatacc
     4321 gtacgacgac gtgtgggcgg gccagaacca ggcgcggttg atcgacaagt ggcgcgagat
     4381 gaccaacaaa taagccaaca tgatggcggc gtcggggcgg actgcttggg cttccggccc
     4441 cggcgctcgc cagcaagcgg ggtggaacag gtgacaactg agcaaaccgg ggccgaacgt
     4501 cttcgcagtc ggcgggcgct aatgatcgtt cgcgagccgg ccgtcgcgat cacaatcatg
     4561 gtcatcgcct cggcgcttgc agtgtttgtc gtgtaccctc tctacatggt attacgcacc
     4621 agccttacga cagatggagg gctgagcctc gcgatcttcc gcagcctgct ttcgagaagc
     4681 tacaaccgaa ggcctcttgc gaacagcctc aagctggggt tagttgtgtc ggcaatgggc
     4741 accgcagcag gtttcatatt cgcctactca actacacggg tggatgtgcc tctccggggg
     4801 ttcttcaggt tagtggccac gtttcccatg atttcgccgc cgctcatcat gtcgctggca
     4861 accatactgc tcttcgggaa caacgggctc atcaccaggc agatccttcg aggcgcagtg
     4921 gactttggga tctatgggtt tcgcggactc gccctggtag aaacattagc ttacttcccc
     4981 actgcctacc tggtactgtc cggagtcttg caggcaatgg acccatcgat cgaagatgca
     5041 gctttggacc ttggcgcatc atggtggcag atgtttcgca cggttacact gccccttgca
     5101 atccccggtc ttgccagcgc gctcctgctc atattcatag aatcgctggc ggacttcggc
     5161 aaccccataa tcctggcggg aaactacact gtgttggcca cccaggccta tctcagaata
     5221 acgggcctat atgacctgtc gggcggcgcg gctctcgccg tgatactcct gatcccctcg
     5281 ctcatcgcct tcttcctgca gaaatactgg gtggggcgaa ggtcatatat tacggtcacg
     5341 ggaaaaccct ccgcggcaag aaccagacgg gacgagccat ggctaagatg gtgcgcgttc
     5401 acactctgcc tcatcctgac cggaatgata ctgcttctct acgggaccgt attcataggc
     5461 tcgttcgtca cgttgtgggg cgcaaattac gagctcacac tcgccaacta caatcacgtg
     5521 ttcagagtag gatggaacca cataagggat acgatctttc tatccacagt atccaccccg
     5581 atcacaggca tactgggcat ggtcattgca tttctcgtcg tccggaagca gttccccggg
     5641 cggcgagcgc tggaagtcgc ttctatgctc acgttcgcca tacccggcac cgttgtcgga
     5701 atcggctata tcctcgcgtt caaccaacct ccgctggtgc tcaccgggac cgccgccata
     5761 atcatcatcc ttttcatctt ccggaacatg cccaccggca ttcagtcggg catagcggct
     5821 ttgcagcaga tagatccatc catcgaagag gcctcgtacg accttggcgc cgactctgcc
     5881 acgaccttca ggaagataac ccttccgctc attgcgccgg cgttcttttc cgggctggtt
     5941 tatagtttcg tgaagtgtgt gacggcaatc agcgcaattg tatttgtggt atcggcgcgg
     6001 tggaagctga tcaccgtggc agtgatgggc tctgtggaga atagtgacct agctcaggct
     6061 gcggcatttt gcgtggtgat catcgcgatc atcctggcgg ccatcggcat cataaatctc
     6121 cttgtatatc ccccgtggag ggcgaggtcg acggcaagag cagcaacggt gggcacgtcg
     6181 ggagcacccg gcgcaattcc tcccggtgtt gcaggagggc agtaaccatg aatcactaca
     6241 gcgtagagct gcaaagcgtc tccaaagttt
//
LOCUS       DAOTAC010000145         6269 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_10546_length_6269_cov_5.533795, whole genome shotgun sequence.
ACCESSION   DAOTAC010000145 DAOTAC010000000
VERSION     DAOTAC010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6269
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10546_length_6269_cov_5.533795"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="PLM74_07195"
     CDS             complement(<1..881)
                     /locus_tag="PLM74_07195"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HON42895.1"
                     /translation="MDVSRFLNSLMSSYEYRGQAVHIEAIPAREASYGRVDPPIAPTL
                     WGALGRMGISSLYTHQAEAVQLAREGRDIVVVTSTASGKTLCYNIPVIEELLRDAKAA
                     ALYVFPTKALAQDQQRGLTRIQELEPSLPVRSGTYDGDTPDSTRRKLREQANIILTNP
                     DMLHQGILPSHPSWRRFFAGLRYVVIDEIHAYRGVFGSNVANVIHRLRRVCAHYGSDP
                     TFICCSATIANPGELAAGICGKPVQVVDDDGAPRGARKFMFWNPPRLEGSMERRSSNS
                     EAERLLVQLIMLGIPTIT"
     gene            complement(1122..2732)
                     /locus_tag="PLM74_07200"
     CDS             complement(1122..2732)
                     /locus_tag="PLM74_07200"
                     /inference="COORDINATES: protein motif:HMM:NF024085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HON42896.1"
                     /translation="MGIQLEHRRLSFSRVSGQGMAEVNTQRSCALPADSPDMSDAQAE
                     AVLWAQGMPVVKSVEPGEGQVKVSGYVRSQVLYIARGEPDWTARVSVEDPRFEVVIPA
                     PGVRPDDVATAEVTIAHFEAESTGARTLQLTAALAVSAQCVRETVVDAAVAAQATGGS
                     RITVHTESVTLSRLVASRSERAQIGETLGIPEGNPPCTVDARSAGAAGMAHVVGVQTS
                     DGKVSVDGELVLEIAYAPARAVAAVNIVRFERVPFHKSFDIPEARKSMGAKARAELAE
                     VSVIPVSDGAFRVDAAVDVSVELATKERVPAVVEIASETQEIVDTETKFIIVEEMVGE
                     GAVDIDVQDTVPVSALAQRRSLTGMEEIRGSLRQVGLSEAEASDGEATVKGLLRTRVF
                     LGDVEEDDAGTFPVAFALEMPVEFSDSVEIPDVTEGDRIEITSVNESVFVERASPAKL
                     DVQGSIRIGVAAYRQTRVSVVTAAELITPISLDPFAMTFYIVTSGDTLARIARRYGVP
                     PDKIALANGIGQTDTLAQGQKLYIPARR"
     gene            complement(2794..4011)
                     /locus_tag="PLM74_07205"
     CDS             complement(2794..4011)
                     /locus_tag="PLM74_07205"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HON42897.1"
                     /translation="MDIRQPHICGMSLLDMQTGLVGLGFDPGPCDGMYGPLTRDAVRK
                     CQAAYRLGATGAPGAEIKRLLGLRLPAFRTTICARAGQSLQALARESGTTAEAIVEGN
                     RRKRYEEVFAGEQLIVHRRAAAVLDGGELRGPEWSFAARQVQTALSALSASSASSAAP
                     GANDASGCFGILSVPASQREEAADMIRQASRAGLRGIVLEAADSDLADDTAWSYIRFV
                     RFVARACRKAGLDLAVSVPVHTRPVGERHSRSSRHVVLRPNGYDLEDVGAVADIVLLD
                     VRDAREPETFRSAIAWACKHIPRWKLMAVIELRPYHMGESGPEPMSGNDLVKLRARHV
                     VREGIDDSTGLPYLAYRARGSVRRLWRESASSLGRKLHVVNRLNILGVAFRGAEEAEE
                     QVLAEIGRRFIIM"
     gene            complement(3978..4994)
                     /locus_tag="PLM74_07210"
     CDS             complement(3978..4994)
                     /locus_tag="PLM74_07210"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HON42898.1"
                     /translation="MHEYRGVIHVHSTYSDGKGCVERITDAANRTRADFVILTDHNTM
                     KAREEGHEGWHRGTLLLVGEEITPDTNADHYLAFGIATAIAPSPDPAKNVEAVRRQGG
                     IGIIAHPTGGAFVKGTYVEYPWTNWEAGTFAGIEIWNHIYDITGKSRNIIQLVLYVLF
                     PWLSPYGPEEGVLETWDYYVSQRGRRVVAIGGVDAHGIIGDYEDDFVTVSTYILTPEP
                     FNGAFGHDSALVYAAIREGRCFVGADKVANASSFRCQVESGGDLVEMGGTASLARGAT
                     LTVSSPKPGLISVIRDGELLAEEWSSSLAIGLDRRGAYRVEVKRKACRRYMPWIFGNH
                     IYVV"
     gene            complement(5175..5453)
                     /locus_tag="PLM74_07215"
     CDS             complement(5175..5453)
                     /locus_tag="PLM74_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HON42899.1"
                     /translation="MAVENVLTKIKEDLDLFVGRTVRLKANQGRKKVLEAQGVLEQTY
                     PKVFVVRIDENPRSVKRVSYSYADVLTSTVEVSVHDSGLDTRIGAMGS"
     gene            complement(5610..>6269)
                     /locus_tag="PLM74_07220"
     CDS             complement(5610..>6269)
                     /locus_tag="PLM74_07220"
                     /inference="COORDINATES: protein motif:HMM:NF017401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HON42900.1"
                     /translation="RITEAQTRQKRDRRRGRDVSVAEDLFDLPGRVLHIDGDPDYLRD
                     CMRYYKELGVPAVGKHVPPEEQPGRLLELLNEFSPDVLVLTGHDALKKKNSDRSSITS
                     YWNSASYVEAVRRARQYEMDRDGLVIVAGACQSFYEAIMEAGANFASSPGRVLIHCLD
                     PVLLAERVVSTPIDDVVRIEDAIENTITKRPGLGGIQTRGKMRASMPRTNMGVFGTGV
                     S"
BASE COUNT         1217 a         2071 c         1697 g         1284 t
ORIGIN      
        1 gtgatagtcg gtatgcccaa catgatgagt tgcaccagaa ggcgctcggc ctcgctatta
       61 gagcttcgcc gctccatgct cccttcaagc cggggagggt tccagaacat gaacttccgt
      121 gcgccacgtg gagcgccgtc gtcatcgacg acctggacag gttttccgca tatgcctgca
      181 gccagctcgc ccgggttggc gattgtcgca gagcagcaga tgaatgtggg atccgacccg
      241 tagtgagcgc acacccgcct aaggcggtgg atcacgttgg caacgttgga gccgaagact
      301 cctctgtagg cgtgtatttc atcgatcacc acgtaccgca gtccggcgaa gaaccgtcgc
      361 cagctcggat gggatggaag tatcccctgg tggagcatgt cggggttggt gagaatgata
      421 ttggcctgct cacgcagttt ccgacgggtc gagtcgggcg tgtcgccatc gtaagtaccc
      481 gaccttaccg gcaggctcgg ctccagttcc tgaattcgcg tcagccctcg ttgctgatcc
      541 tgggctagag ccttggtggg aaagacgtac agtgctgcgg ctttcgcatc ccgcaagagc
      601 tcttcaatta cggggatgtt gtagcagaga gtcttgccgc tggctgtcga tgtaacgacg
      661 acgatgtcgc gcccttctct agctagttgc actgcctctg cctggtgagt gtagagagac
      721 gatatcccca tgcgccccag agcgccccag agcgtcggag ctatcggcgg atccacccgg
      781 ccgtaactgg cctcccgggc ggggatggcc tcgatgtgca cagcctggcc gcgatattcg
      841 tagctggaca taagtgagtt gagaaatctg gaaacgtcca accgcccgtt cctcgctttc
      901 ctagccttcc cagcaaccgc cgagcgcgcg atcccgatcc cggtcccgat ctcgatcccg
      961 gttcgcggca tggcgcggcg gcatgttccg cactattgtt cgcctgtata gtgccgaatt
     1021 cctccacgaa cgaaaccgtc ccccctgcgc cgccgcggca agccgtaacg ggctgcggcg
     1081 cgaagagggg acggcaagat ctacccgcgc tatcctacat cctacctacg tgcagggatg
     1141 taaagcttct gaccctgcgc aagtgtatcc gtctggccta tcccgttggc caacgctatc
     1201 ttatccggag gaacgccata cctgcgcgca atccgcgcga gcgtgtcgcc agaggtcaca
     1261 atgtaaaagg tcatcgcgaa cgggtcgagc gatatgggcg tgatcagctc ggctgccgtc
     1321 accactgata cccgggtttg gcggtatgcg gcaacgccga tgcgaatgga accctgcaca
     1381 tcaagtttgg ccggactggc cctttccacg aacaccgatt catttacaga cgtgatttcg
     1441 atcctgtctc cctcggtcac gtccgggatt tcgacggagt ccgagaattc caccggcatc
     1501 tccagcgcaa aggcaaccgg gaacgtgccc gcatcgtctt cctcgacatc gccaaggaac
     1561 actctggtcc tgagcagtcc tttcaccgtc gcctccccat ctgacgcttc cgcctccgaa
     1621 aggcccacct gccgcaggct tccccggatc tcctccatgc ccgtgagcga acgacgctgc
     1681 gccagcgccg acacaggcac tgtatcttgc acatcgatgt cgacagcgcc ttcgcccacc
     1741 atctcctcaa cgatgatgaa cttggtctcc gtatccacta tctcctgtgt ctccgaagct
     1801 atctcgacga ccgccggcac gcgctccttg gtcgcgagtt caacgctgac atccactgct
     1861 gcgtccaccc ggaaagcgcc atccgacacg ggtatcacgg agacctctgc cagctcagcc
     1921 cgggcctttg cgcccataga cttacgggcc tccggaatat caaaggactt gtgaaaaggc
     1981 acgcgctcga acctaacgat gttcaccgcc gccaccgctc tcgccggagc gtaggcgatc
     2041 tctagcacca gctcaccgtc gaccgacacc ttgccgtcgg atgtctgaac acctacgacg
     2101 tgggccattc ccgcggcccc ggcggacctg gcatccacag tgcacggcgg gttaccctca
     2161 gggataccga gcgtttcccc aatctgcgcg cgctccgacc tcgacgccac aagtctgctc
     2221 aaggtcacgc tctcggtgtg gactgtgatg cgcgacccgc cggtggcctg agcagcaaca
     2281 gcggcatcca caacggtctc cctgacgcat tgggctgaaa ccgccagagc cgcggtgagc
     2341 tgcaacgtgc gggcgccggt gctctcggcc tcgaaatgcg caatcgttac ctcagcagtg
     2401 gcaacatcgt caggcctgac tcccggggct ggaatgacaa cctcgaacct gggatcctcg
     2461 acggagaccc tcgccgtcca atcgggttcg ccgcgggcta tgtacagcac ctgcgatctg
     2521 acgtacccgg atactttaac ctgtccttcc ccgggctcca cagacttcac gaccggcatt
     2581 ccctgagccc agaggacggc ctccgcctga gcatccgaca tgtccggaga atctgcaggc
     2641 agcgcgcagg accgctgggt gttgacctca gccatgccct gcccggacac cctggaaaat
     2701 gagagcctcc tatgctcgag ctgaattccc acttgttcct cccccctggc gttggcgggt
     2761 ctagccgccc tctgccagaa tatatgccgg gagtcacatg atgatgaacc ttcggccgat
     2821 ttccgcgagg acttgctctt ctgcctcctc cgcgccccga aaagccaccc ccagtatgtt
     2881 aaggcgattc accacgtgaa gctttcggcc aaggctgctc gcgctctcgc gccataaccg
     2941 tcggaccgag ccgcgggcgc ggtaggccag gtaaggaagg cccgtcgagt cgtctatgcc
     3001 ctctcgcact acgtgtctag ctctcagctt gaccagatca ttcccagaca tgggctcagg
     3061 ccccgattcg cccatgtgat acggcctcaa ctcgatcacc gccatcagtt tccaccgcgg
     3121 aatgtgcttg catgcccagg cgatcgcgct ccggaaggtt tccggttcgc gtgcatcccg
     3181 gacatcaagg agtacaatat cggccaccgc ccctacgtct tcaagatcgt atccattggg
     3241 gcgcaataca acatggcgac ttgagcgact gtgacgctca cctacgggcc gagtatgaac
     3301 gggtacactt accgccaggt caagcccagc ctttcggcac gcacgcgcta cgaacctgac
     3361 aaaccgtatg taggaccatg cagtgtcatc agcaagatcc gagtcggcag cctcaagtac
     3421 aatgcctcgc aggcctgctc ggctcgcctg ccgtatcatg tcggcagcct cctctcgctg
     3481 gctcgccggc accgacagga tcccaaaaca gccactagcg tcatttgcgc cgggcgctgc
     3541 cgacgatgca gacgacgccg acaacgccga cagcgcggtc tggacttgtc gggctgcgaa
     3601 gctccactcc ggcccgcgca gttcgccgcc atcaagcact gcagcagcac gtctatgcac
     3661 aatcagctgc tccccggcaa acacctcttc gtatctcttt cgcctgttgc cctcgacaat
     3721 cgcctctgcc gtcgtgccag actccctggc gagtgcctgc agcgactggc cggcgcgcgc
     3781 acagatggta gtcctgaacg caggcaaacg caagcccagt agtcttttga tctcagcacc
     3841 cggcgctccg gtcgctccga gcctgtaggc tgcctggcat ttccgtaccg catcccgggt
     3901 aagcggaccg tacattccgt cgcacgggcc agggtcaaac cccagcccga cgaggcctgt
     3961 ctgcatgtcg agaaggctca taccacatat atgtggttgc cgaatatcca tggcatgtat
     4021 ctcctgcacg ccttccgctt gacctcgacg cgataggcac cgcgccggtc gagcccaatc
     4081 gccaggctgc tgctccattc ctccgccaga agctcaccat cacggatcac actgatcagc
     4141 ccgggtttcg gcgatgatac agtgagggtc gccccgcgcg ccagacttgc cgtgccgccc
     4201 atctccacca agtcgccgcc tgactccact tggcagcgaa aggacgacgc attggccact
     4261 ttgtcggctc ccacaaagca cctgccttcg cgaatagcag cgtacaccag tgctgaatca
     4321 tgtccgaaag cgccgttgaa cggctcggga gtgaggatgt acgtggacac ggtgacgaag
     4381 tcgtcttcgt agtcgcctat tatcccatgg gcatccacac cgccgatagc cactactctc
     4441 ctacccctct gggacacgta gtaatcccag gtctcaagga cgccttcctc agggccgtac
     4501 ggtgaaagcc aggggaaaag aacatacagc accagctgga taatgttcct ggatttgccg
     4561 gtgatatcgt aaatgtggtt ccaaatctct attccggcaa atgtcccagc ttcccaattg
     4621 gtccacgggt actcaacata tgttcccttg acgaacgcgc ctcctgtggg atgggctatg
     4681 atgcctatcc cgccctgcct gcgcacggct tcgacattct tggccgggtc gggcgacggc
     4741 gcaatggcgg tagcaatgcc aaaggccagg tagtggtcgg cattggtgtc gggggttatc
     4801 tcctctccca cgagcagaag agtgcctcgg tgccagccct catgcccttc ttccctggcc
     4861 ttcatggtat tgtggtcggt cagtatgacg aagtcggcac gtgtcctgtt ggcagcatca
     4921 gtgatcctct cgacgcagcc cttgccgtcg gagtaggtgc tgtggacgtg aatcacgcca
     4981 cggtattcgt gcatgtagcc tatccgcccc ttcaccgcga gactgatgtg cgctgcagat
     5041 tatctatatt cctccacagc gccccgcgtg aaagagcagg ggtggcctac gatatgcctg
     5101 aaaatgaaga aaccccccga ctgctcaggg gggttctcac gatttgtaac gggtgtatgc
     5161 cgggtatccc gaaatcagga gcccatagca cctattctgg tatccagacc gctatcatgc
     5221 acgctcacct cgacggttga ggtaaggaca tctgcgtagc tgtaagaaac cctcttcacg
     5281 ctccgcgggt tctcatctat gcgtacgacg aataccttgg gataggtctg ttccagaaca
     5341 ccctgggcct ctaagacctt cttgcggccc tgattggcct tgagccttac ggtcctgcct
     5401 acgaacaggt caagatcctc tttgatcttg gtgaggacgt tctccaccgc caacgacttc
     5461 accttccttc ccaacctcgt cttttgtaat ggtatcacga agtgggatgg aagtcaagcg
     5521 aaataggtat tataccaaag cgcagcaatg gctgtcaaga acctcggatc acttttttgg
     5581 ccgcagccca cccgcagccg gctcagttcc taactcacac ccgtcccaaa cacgcccata
     5641 ttcgtccgtg gcatgctggc gcgcatcttg cccctggtct ggattccgcc tagcccgggc
     5701 cgcttggtga tagtgttctc tatggcgtct tctatccgta ctacgtcgtc gatcggtgtg
     5761 ctcacaaccc gttcagccag caagaccggg tcgaggcagt gaatcagcac gcgccccggc
     5821 gaactcgcaa agttggcacc tgcctccatg atggcctcat agaaagactg acacgcccca
     5881 gccactatga ccagaccatc cctgtccatc tcgtattgcc gggcacggcg caccgcctcc
     5941 acgtaagatg ccgagttcca gtagctcgtg atggacgacc gatcagagtt cttctttttg
     6001 agcgcatcat gccctgtgag gacaagcaca tccggcgaaa actcattcag cagttccagg
     6061 agtcgcccag gttgctcctc gggcggaacg tgcttgccta ctgccggcac gcccagttcc
     6121 ttgtagtatc tcatgcagtc tcgcaaataa tcggggtcgc cgtcgatgtg aagcaccctg
     6181 cccggcaagt cgaaaaggtc ttcggccacg ctcacatccc tgccgcgacg ccggtcccgc
     6241 ttctgcctcg tctgcgcttc ggttatgcg
//
LOCUS       DAOTAC010000146         6265 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_10554_length_6265_cov_4.385185, whole genome shotgun sequence.
ACCESSION   DAOTAC010000146 DAOTAC010000000
VERSION     DAOTAC010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6265
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10554_length_6265_cov_4.385185"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..179)
                     /locus_tag="PLM74_07225"
     CDS             complement(<1..179)
                     /locus_tag="PLM74_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007488053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HON42901.1"
                     /translation="MHKIAVIGDEDSILGFRALGVSVFPVRSGEEADAALRAAARQDY
                     AVIYITEGYAGEIIDT"
     gene            complement(172..1257)
                     /locus_tag="PLM74_07230"
     CDS             complement(172..1257)
                     /locus_tag="PLM74_07230"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HON42902.1"
                     /translation="MGFDARFAYVVGRVRALENRMIDQARFNRMIDAEGPEELARILS
                     ETEYSLARDLGPTEYEEIIDGELTRVHSLIEGISPDPVLTGVFRARHDFHNMKAHLKA
                     RLARRDRGVVVEESSGVTRLGRLDPARLAVVADALVLGSDEGDVEPESDGKRWSTRGS
                     ADDLEGAVADAARRAVFAYEANGRDPQYIDLVLDRESFEYFYRVARSRRADFLIGLVQ
                     AMADLTNILICMRLRLIGKPADFARDALLPRGSLDTEELLAVYSEPDDSFWSFFKRTR
                     YDRLVEEALAAWRDRGSLSAFERVVRAGLAREAAAGSMADMGYEPVLAYLMAREGEAD
                     ILRRIFVGKMNRLPAEVIRERLCGVHA"
     gene            complement(1263..1865)
                     /locus_tag="PLM74_07235"
     CDS             complement(1263..1865)
                     /locus_tag="PLM74_07235"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HON42903.1"
                     /translation="MHNLDRLVARITDEARSKADDILRDADARCAALAGESRKKADDT
                     YRMAVDRATLAAAEEERRAQISRSLDVRNAVLRAKGDMVDALMSEIPARIQALPDQEY
                     LALMKMMMIDAAPDGHVEVVVAEPDRNRINAAFVAAVQSELASRGRDSKLRLAGDTLQ
                     LKGGFVLRASALEVDCSLDAILAARGDELAPLVAEVLLGR"
     gene            complement(1885..2364)
                     /locus_tag="PLM74_07240"
     CDS             complement(1885..2364)
                     /locus_tag="PLM74_07240"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012269824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HON42904.1"
                     /translation="MEGFLGTILGFLGVALAIITPGIGSSVGVGLVGRSGAGVITEDP
                     DKFGSILLLEAIPGTQGVYGFLTGLMIMIRIGALGGGLRTLTVGQGILFIMAAMPIAV
                     VGWVSAIFQGRVAAAGVGIVAKRPEEMGKAVILAAMVETYAVLSLLASLLLVFSVNV"
     gene            complement(2368..4326)
                     /locus_tag="PLM74_07245"
     CDS             complement(2368..4326)
                     /locus_tag="PLM74_07245"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HON42905.1"
                     /translation="MRRVAVLGHESRIGDVVTTLQRLGTVEVSADTADLSCPFNPAAG
                     LAHVDSVMSELKYSIDIMGRHRRISKTAIESFTGYRLYLTDDEWTMYVRDGAENAHRI
                     YAMARAVEQSLADLAAAEARILTSIEYLEPWRELRVPVEQVVPGKYVGLALGTAESRS
                     YEAALADAAEAVEGMSWEIVTAEKDKVRLLVAYPMSAADDALRALAQAGFAKVSFEQY
                     SGAPSDIIAQLEQDLQSIQAQRAQVERDVDILVEERPRAFALYDFFALERARLDVTNK
                     FGGTERTFVVEGWIQQANLQGFRTAIEQLADDIVVLDRPPREDEEPPVELENNRFASA
                     FEPVTKMMGYPKHGSLDPSPFLGPFFLIFFGLCMTDAAYGIVIAALAYWLIGQTRARG
                     MGRQFLNLLIMSGLATVFVGAMLGGWAGDLLQRLFGWRTAILFDPMEEPTKFLILSFA
                     LGVIQIYTGIILKAYDNIRRGDWMSAIFDQVFWLVFLTSIGLALGGGYLGPSGPAIAS
                     AGQKLLLASAIGLVLTQGRHHKNPLMRIGSGLLSLYNTTGYMSDIVSYARLFGLGFTS
                     TVLASVMNDLMLRPAGIPVIGPIIALIGLVFGHLFNILINIIGAYVHSSRLQYVEFFG
                     KFFEAGGRRFMPFGMSTKYVDVENAKGA"
     gene            complement(4328..4654)
                     /locus_tag="PLM74_07250"
     CDS             complement(4328..4654)
                     /locus_tag="PLM74_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42906.1"
                     /translation="MSIEALDRIRAAEREADEIVRNAHDEAKARIARAELDGRRAMQQ
                     AEEKARTKAEEISSAARARIEAEACDARKVIAARVDEVNSTAKANWEKAMDLAVRKVL
                     NSFGDL"
     gene            complement(4808..5647)
                     /gene="spo0A"
                     /locus_tag="PLM74_07255"
     CDS             complement(4808..5647)
                     /gene="spo0A"
                     /locus_tag="PLM74_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HON42907.1"
                     /translation="MTREKTRVLVIENNKDLCEVIEALLREEEDMELVGVAHDGVLGL
                     DMIAQTRPDVVLLDIIMPYLDGMGVLSQLDDLGLERRPRIVVLSAFEEEDMVRRCARL
                     GADYYLLKPFDRDNLISRIRQVGQADVVQAKPKAERAYAGRERPREREADRRPRAAQF
                     DLETEATRILYNLGVPTYFKGYCYIKEAIVLVTKDIGLLGAVTKSLYPKVAEKYGTTP
                     LIVEAAIRYTIQKTWKQGNPDQINKVFGYAVNLRNGKPPTNSFFIAKIAEELRLEMLE
                     HAQ"
     gene            complement(5935..6009)
                     /locus_tag="PLM74_07260"
     tRNA            complement(5935..6009)
                     /locus_tag="PLM74_07260"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5974..5976),aa:Arg,seq:ccg)
BASE COUNT         1288 a         2035 c         1684 g         1258 t
ORIGIN      
        1 gtgtcaatga tctcgcctgc atagccctcg gtgatgtata tcacagcata gtcctggcgg
       61 gcagccgcac gcagcgccgc atcggcctct tcgccgcttc gtacggggaa gaccgacacg
      121 cccagagccc gaaagcccag gatcgagtct tcgtcgccga taaccgcaat cttatgcatg
      181 cacaccacac agcctctcgc ggatgacctc cgcaggcagc ctgttcatct tgcccacgaa
      241 aatgcgccgc aatatgtcgg cctcgccctc gcgcgccatc aagtacgcca gcaccggctc
      301 gtagcccata tcggccatgc tgccagccgc cgcctcgcgg gccagccccg cccggaccac
      361 gcgctcgaat gccgaaagag acccgcgatc gcgccaggcg gcgagggcct cctccaccag
      421 cctgtcataa cgagtccgtt tgaaaaagga ccagaacgag tcgtccggct cgctgtacac
      481 ggcgagcagt tcctccgtat ccagactgcc tcgggggagc agcgcatccc gcgcaaaatc
      541 ggcgggtttg cctatcaggc gcaacctcat acatatcagg atattggtaa ggtcggccat
      601 ggcctgaacc agacctatga ggaagtcggc ccgccgcgac cgggcaacgc gatagaagta
      661 ttcgaatgat tccctgtcta gcaccaggtc aatatactgc ggatcgcgtc cgttcgcctc
      721 atatgcgaat acggctcgcc gggcggcatc ggccactgcg ccctccaaat cgtcggcgct
      781 cccccgtgtc gaccagcgct tgccatccga ttccggctcc acgtccccct cgtcactgcc
      841 caaaaccaac gcatcagcaa caaccgccag ccgggcagga tccagcctac caaggcgcgt
      901 gaccccactg ctctcttcca cgacgacccc ccggtcgcgc cgggccagcc tggccttgag
      961 gtgcgctttc atgttgtgga agtcatgccg tgcgcgaaac acgcccgtga ggacgggatc
     1021 gggcgagata ccctcgatga gcgaatgcac tcgcgtgagt tcgccgtcga ttatctcctc
     1081 gtattccgtc ggccccagat cgcgggcgag agaatactca gtctcgctca gaatccgcgc
     1141 gagttcttcg gggccctcgg cgtcgatcat cctgttgaac cgggcctggt ctatcatacg
     1201 gttctcgagc gcccggactc ggccgacgac gtacgcgaac cttgcatcga agcccacggc
     1261 gcctacctcc caagcaatac ttccgccacc agcggggcaa gctcgtcgcc tctggcggcc
     1321 agtatcgcgt cgagcgagca gtcgacttcc agggcgctcg cgcggagtac aaaaccgccc
     1381 ttcagctgaa gcgtgtcgcc ggcgagcctg agcttcgaat cgcgtccacg cgatgctagt
     1441 tcagactgga ccgctgccac aaaagcggcg tttatgcggt tccggtcagg ctcggctacg
     1501 actacctcca catggccgtc cggcgctgca tctatcatca tcatcttcat gagcgccagg
     1561 tactcctgat ccggaagggc ctgtattcga gcgggtatct cggacatgag cgcatcgacc
     1621 atgtctcctt tggcgcgaag gacagcattg cgcacatcga gagagcgcga gatctgcgcc
     1681 cgcctctcct cctcggccgc cgccagagtg gcccggtcga cggccatcct gtatgtgtca
     1741 tcagccttct tcctgctctc accggccaaa gcggcgcagc gtgcgtcggc atccctcagg
     1801 atatcgtcgg ctttgctccg cgcctcgtcg gtgatgcggg ctaccagcct atccaggtta
     1861 tgcatgtcat caactcccgg gcaatcagac gttcaccgag aagaccaaga gcaggctagc
     1921 aaggagcgac agcactgcgt aggtttcgac catggctgcc aggatgacgg ccttgcccat
     1981 ctcctcaggg cgcttggcga caattcccac gccggctgcg gcaaccctgc cctggaatat
     2041 ggccgatacc cagcctacca ctgcgatggg catggcagcc atgatgaaca gaataccctg
     2101 ccccaccgtg agcgtgcgca acccgccgcc gagagcgcct attctgatca tgatcatcag
     2161 ccctgtgaga aagccgtaaa caccctgtgt gcccgggatg gcctcgagca gcagaatcga
     2221 accgaacttg tcgggatctt cagttatgac ccctgctccc gaccggccca ccaaccccac
     2281 gcctactgac gaaccgattc caggcgtgat gatcgccaga gctactccca agaagcccag
     2341 aattgtgccg agaaagccct ccatgtgcta tgcccccttc gcgttttcca catcaacgta
     2401 ctttgtagac atgccaaacg gcataaatct gcgccctcca gcttcgaaga acttgccgaa
     2461 gaattccacg tactgtaaac gactggagtg gacgtaagcg cctatgatgt taatgagtat
     2521 attgaacagg tggccgaaga ccaggccaat cagggcaatg atcggcccta tcacaggtat
     2581 cccggcaggc ctgagcatga ggtcgttcat gaccgaggct agcaccgtgc tggtgaaccc
     2641 gaggccaaat agcctagcat acgatactat gtcgctcatg tatccggtcg tgttgtacag
     2701 cgacagcagg ccggatccga tgcgcatcag agggttctta tgatgcctgc cctgtgtcaa
     2761 cacaagacct atcgccgagg cgagcaggag tttctgcccg gccgaagcaa tggcaggccc
     2821 gctcggaccc aggtaccctc ctcccagcgc caggccgatg cttgtaagga acacaagcca
     2881 gaatacctgg tcgaatatgg ccgacatcca gtcgcctcgc cggatgttgt cgtaagcctt
     2941 gaggataatg cccgtgtaga tctgaattac gccgagtgcg aacgagagaa taaggaactt
     3001 tgtgggttcc tccatcgggt cgaacaggat ggctgtgcgc cagccgaaaa ggcgctgcag
     3061 aagatccccc gcccaacctc ccaacattgc gccgacaaac accgtggcca gcccgctcat
     3121 gatgagcagg ttgaggaatt gccggcccat gccgcgggcg cgagtctggc ctatgagcca
     3181 gtatgccagg gctgcgatga ctatgccgta tgcggcgtcg gtcatgcaca gcccgaagaa
     3241 tatcaggaag aacggcccca gaaacgggct ggggtccagc gaaccgtgct tgggatagcc
     3301 catcatcttg gtaacgggct cgaaagctga ggcgaatctg ttgttctcca gctccaccgg
     3361 tggttcctcg tcttcgcgag gaggacggtc gagcaccacg atatcgtccg cgagctgttc
     3421 tatggccgtg cggaacccct gcaagttcgc ttgctgtatc catccttcga cgacgaacgt
     3481 acgctccgtg ccgccaaact tgttcgtcac gtcaagcctg gcacgctcaa gcgcgaagaa
     3541 gtcatacagc gcaaatgcgc gcggcctctc ctccaccagg atgtcgacat cccgttcgac
     3601 ctgggccctc tgggcctgaa tcgattggag atcctgctct aactgggcaa ttatgtcaga
     3661 cggggcgcca ctatactgct cgaagctcac cttggcgaac ccggcctgtg cgagagcgcg
     3721 aagcgcatca tcagcagccg acatgggata cgcaaccagg agacggacct tgtccttctc
     3781 ggcggtgacg atttcccacg acatgccctc gactgcttcg gccgcgtcgg ccagagcagc
     3841 ctcgtacgaa cgcgactcgg ctgtgcccag ggccagaccg acgtacttgc ccggcaccac
     3901 ctgctcaaca gggacgcgaa gttcccgcca tggctctagg tactcgatcg atgtgaggat
     3961 acgagcctcg gcggcagcta agtcggccag cgactgttcg accgcccttg ccatagcgta
     4021 gatgcgatgg gcgttctcag cgccgtcccg aacatacatc gtccattcat catcggtgag
     4081 gtaaagacgg tagccggtga agctctcgat agcggtcttg gatatgcggc gatgccggcc
     4141 catgatgtcg atggaatact tgagttccga catcaccgag tctacatgcg caaggcccgc
     4201 tgcggggttg aagggacaag aaaggtcagc cgtgtcggcg ctcacttcga cggtgcccaa
     4261 acgctgaaga gtagtgacta catcgccgat tcgggactca tggcccaaaa cggctactct
     4321 tctcatctca gagatcgcca aacgagttca acaccttccg gaccgcgaga tccatggctt
     4381 tttcccagtt ggccttcgcg gtgctgttca cttcgtccac acgcgctgcg ataaccttcc
     4441 tggcgtcgca tgcctcggcc tcgatacggg cccgggcagc cgaggagatc tcctcagcct
     4501 tcgtgcgggc cttctcctcc gcctgctgca tagctcgcct tccgtcgagt tcggctcggg
     4561 cgatgcgcgc ctttgcctcg tcgtgggcgt tgcggacgat ctcgtcggcc tcccgttcag
     4621 cagctcggat acggtctagc gcctcaatgc tcatgcgggc acacccccag attgggcaag
     4681 aaagacgaat catgcctctt aatctaactg gcattatact acactacata ctagtccgca
     4741 atcacagaag cggcgggtcc ctcatcgggt ccgccgctca cataggcacg tatatatcgg
     4801 cttattccta ctgcgcgtgc tccagcattt ccaaacgaag ctcctcggct atcttggcaa
     4861 tgaagaacga atttgtgggg ggcttgccgt tgcgcagatt aacagcatat ccgaacacct
     4921 tgttgatttg gtccggattg ccctgcttcc aggtcttttg aatggtgtac ctgatggcag
     4981 cctcgactat gaggggagta gtgccgtact tctcggcgac cttcgggtag agcgacttag
     5041 tcactgcgcc cagaagaccg atatccttgg tgacaagaac aattgcttcc tttatatagc
     5101 aatatccttt gaagtaagtc ggcactccaa ggttgtacag aatccgggtt gcctcagtct
     5161 cgaggtcgaa ctgcgccgcc ctgggcctac ggtcggcctc tcgctcccga gggcgttcac
     5221 gtccggcata tgctctttcc gccttgggtt tcgcctggac tacgtcagct tggcccacct
     5281 gccgtatgcg gctgatcaag ttgtctcgat cgaacggctt cagcaggtag taatcagcgc
     5341 ccaatcgcgc acaccttctc accatgtctt cctcttcgaa ggccgacaac accacaatcc
     5401 gagggcgccg ctctagcccc aagtcatcca gctggctcaa tacgcccatc ccatcaagat
     5461 aaggcatgat aatgtccaga agaacaacat ccggcctggt ctgggcgatc atgtcaagac
     5521 ccagcacccc gtcgtgcgcc acacctacaa gctccatgtc ctcctcttcc cgaaggagag
     5581 cctcgattac ctcgcagagg tccttattgt tctcgatcac cagaaccctg gtcttctccc
     5641 tcgtcaagct aattcccccc aaattcgcgt acatactgga gtgccgtcga ttatcgcaac
     5701 tgaactattc tccggctcga atgagactcc tcccctcatc ctgcaattta tggcggtatt
     5761 ttatcatatt tctcgacatc tcagccggga tttgggtcga atgttgccgg ttaggcacga
     5821 ttgcacgctc ccttgcgcgg aatcatcgta acattggctc gaacatcacg atatttgaag
     5881 ccctcctgca gccgacgcac caacgcatcg acataaggag ggcttcaaga aaaatggcgc
     5941 gcctagcagg aattgaacct gcgcacccgg ctccggaggc cggtgctcta tccactgagc
     6001 tataggcgca cacacacccg tattcatctg tacatttgca ttatacgctt ccctgcctcg
     6061 gttcgcaagg ccactgcctg gatgctgcct ggatgcttcc gacctgggcc gtcacacctg
     6121 ggtcactgct atccccagca tgcccgggcc gccatgcacg gccatggatc ctcccatctc
     6181 agaaaagtgc gcctcaccga agtcgatggc ctgcccggcc tgctcaagca gagcacaggc
     6241 ctcgtccagc gcattagtat gcatg
//
LOCUS       DAOTAC010000147         6198 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_10686_length_6198_cov_5.632264, whole genome shotgun sequence.
ACCESSION   DAOTAC010000147 DAOTAC010000000
VERSION     DAOTAC010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6198
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10686_length_6198_cov_5.632264"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1310
                     /gene="glmU"
                     /locus_tag="PLM74_07265"
     CDS             <1..1310
                     /gene="glmU"
                     /locus_tag="PLM74_07265"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HON42908.1"
                     /translation="GKRMKSSLPKALHQVAGQPMIDHVVGTLIESGASRVITVVGHEG
                     RILQEHLAGRSEVVWQHDQLGTGHAAAQAGPALAGYDGNIVVVPGDVPLLSASAIQDL
                     AREHETHGLAATVLTMVVDDPAQYGRVVRDPSGRVSSIVEYRDASDEIRAVREVNTSV
                     YCFRSHDLFDALPLLSNDNQQGEYYLTDVISVLISRNLPVGAISLDDPSEGMGINSRA
                     ELAAAEQAMRDRKRRELMEDGVTLVDPASTFVDMQVKVGRDTVIMPFTVISGSTSVGE
                     SCNIGPYVCIRDSIVGNGCNVGPFAFVRPGTVLGDRVKVGDFVEVKNSRVGEGTKIPH
                     LSYIGDAVLGAGINIGAGTITCNYDGVTKHTTVIDDGAFIGANSNLVAPVRVGQNAYI
                     ATGSTVTTDVPAEALAIARARQENKDGWVARRKAAQAAKEEKK"
     gene            1310..2257
                     /locus_tag="PLM74_07270"
     CDS             1310..2257
                     /locus_tag="PLM74_07270"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003247441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HON42909.1"
                     /translation="MLSCHRKLKVFACNSNPEMAEEIAVNLGTTLVKSCVGRFSDGEI
                     RVQIHESIRGADVFVIQSLSRPVHDNIMELLIVCDALRRASARSITAVVPYYAYARQD
                     RKAQPREPIAAKLLANLITAAGATRVLTIDLHAPQIQGFFDIPVDHLKAAPILAQHLD
                     ARGLRGCVIVSPDVGGVTRARVIAEHLDATLAIVDKRRPRPNVSEVMNIIGEVKGKPC
                     VIVDDLVDTAGTIVQAAAALLDAGATEVRACCTHGVLSGPAIERLKSSVIQELVITNT
                     IPVSAHDNIDKITVLSVAPLFAEAIRRIYEELSVSILFE"
     gene            2330..2887
                     /gene="pth"
                     /locus_tag="PLM74_07275"
     CDS             2330..2887
                     /gene="pth"
                     /locus_tag="PLM74_07275"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HON42910.1"
                     /translation="MFLIIGLGNPGSRYECTRHNVGFMVLDRLGRELGVAFRKAPYHS
                     LATRVGVSGNDLVLIKPQTYMNNSGIAVAGATRWHKVSPESLIIVYDDMDLEVGRLRI
                     RARGSAGGHNGMKSVVEHLGTEEIVRLRLGIGRPEPWDDPVDYVTGLFRRDELPAMSD
                     AIMCAVRAILCIVEEGVDVAQTRFN"
     gene            2981..>6198
                     /gene="mfd"
                     /locus_tag="PLM74_07280"
     CDS             2981..>6198
                     /gene="mfd"
                     /locus_tag="PLM74_07280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HON42911.1"
                     /translation="MTGILNTLLESGTFSDVLSRIARGANKAQIAGLSGSRKSLFIAG
                     LLWHTRRTVFVVTPNQLEADAIAGDLTALTGQDRVFVFPPVDMMPYEEAPLSADLAAA
                     RTRALVSLMEVGRQCAIVAPVRSLGKRIVPISVFREHVFRLRQGETCDLDELADRLVS
                     GGYDIHAKVESRGQASVRGGIIDVFPHDADSPARIELFGDEIDSIRSFDVNTQRSTGL
                     MDEVLITPAREFLYDSERLGRACDAIRGELERVGSPQLRERVIHHLERFENGARFDNE
                     EQYLPFFYPERVAPVDWVPNALFIIDEPARVEEALDGYEADVRETYASLLQAGMVLPS
                     QAESYLSAHDIAASIDRRQAVSMSILSREIGATPGTTAGTGTEDAQLIAPVKQADLYA
                     GRFEDLVHDIKKYRKRQYRVVCTVSTADRRDRLAETLEDSGVPVTKLATLSEVPDQGS
                     VSVVTAEMTSGFQYPDLKLLLLTDAEIYGRQKKRRLFAAAEEGARIASYTDLTPGDYV
                     VHVNHGVGRYSGVQRLEVGGVQRDYLLVQYAGEDRVYVPTDQVAMLQKYIGMEDAAPR
                     LNKLGGGEWTRAKNRVRESARDMAQNLIKLYAARGSVKGYSFSRDTVWQQEFEDAFQY
                     EETPDQWRAIVDVKRDMEKALPMDRLLCGDVGFGKTEVALRAAFKAAADGKQVAVLTP
                     TTILAQQHYNTFTERFRGFPMRIAMLSRFQTEAEQREIVRGIKLGSVDVVIGTHRLLS
                     QDIAFRDLGLLVVDEEQRFGVVQKERIKELRKEVDVLTLTATPIPRTLHMAMVGVRDM
                     SLIETPPENRFPIRTYVLEQNETLIREAVLREVDRGGQVYFVHNRVQTIDEAAAVLAR
                     LVPEVSIGVAHGQMPEDRLERVMLDFLASEFDVLVCTAIIESGIDIPNVNTIVINDAQ
                     NFGLAQLYQLRGRVGRTNRVAYAYLLFRRDLVLTELAEKRLAAIREFTSLGSGYKIAL
                     RDLEIRGAGNILGAEQHGHIAAVGFEMYCRLLEEEIRELKGEAKRPEPAQVMIDLPVD
                     AFVPDSYVSDSRQKIDVYKKINGVDTMEDAADLTREFVDRFGP"
BASE COUNT         1323 a         1614 c         1964 g         1297 t
ORIGIN      
        1 ttggcaagcg aatgaagtca tccttgccca aagcgcttca ccaagttgcc gggcagccca
       61 tgattgacca cgtagtcggt acgcttatcg aatccggcgc ctcgcgagtg atcactgtgg
      121 ttggccatga aggccgcatc ctccaagagc acctggcagg gcgatctgaa gtagtatggc
      181 aacacgacca attgggcacc ggccacgccg cggcacaggc agggccggcg cttgctggtt
      241 atgatggaaa cattgtcgtt gtgcccggtg acgttcctct tttgtcagca tctgctattc
      301 aggaccttgc ccgggagcat gaaactcatg gattagctgc cacagttctc actatggtag
      361 tggatgatcc cgctcagtac ggcagggtcg tcagggatcc gtcaggccgc gtgagttcta
      421 tagtagaata ccgcgatgcg tcggatgaga ttcgtgctgt gcgcgaggta aacaccagcg
      481 tttactgctt ccgcagccat gatctgtttg acgcgcttcc tttgctgtca aatgataatc
      541 agcagggcga gtactatctc actgatgtga tatccgtcct gatctcccgt aacctgccgg
      601 ttggcgcaat ctcgctagat gatccgtccg aggggatggg catcaactcc cgggcggagc
      661 tagccgccgc tgaacaggcg atgcgcgacc ggaaacggcg ggaactcatg gaagatggtg
      721 taacactcgt ggatccggcg agtacgttcg tcgacatgca ggtcaaggtg gggcgcgaca
      781 cggtgatcat gccgttcacc gtcatatcag gcagtacatc cgttggcgaa tcgtgtaata
      841 tcggcccata tgtgtgcatt cgcgactcga tcgtaggtaa tggatgcaat gttggcccat
      901 tcgccttcgt gcgccccggt acggtactgg gcgacagggt caaggtgggc gatttcgtgg
      961 aggtaaagaa cagccgtgta ggtgagggca caaagattcc acacctcagc tacatcggag
     1021 atgcagtgct tggagcgggc atcaacatcg gcgccggcac aattacctgc aactacgatg
     1081 gcgtgactaa gcacacgact gtcattgatg acggcgcttt catcggagca aactctaact
     1141 tggttgctcc tgtgcgggtg gggcagaatg cttacattgc gaccggttca acggtgacga
     1201 ccgatgttcc ggccgaggcg cttgccatag cccgggcgcg tcaggagaac aaggacgggt
     1261 gggtcgcgcg gcgaaaagcg gcccaggctg ccaaggagga gaagaagtaa tgctttcctg
     1321 ccaccggaaa ctgaaggtct ttgcatgcaa ctccaatccg gagatggcgg aggagatcgc
     1381 ggtcaacctg ggcactacgc ttgtgaaatc gtgtgtcgga agattcagcg acggcgagat
     1441 acgtgtgcag atacatgaga gcatcagggg cgcagatgta ttcgtgatcc agtcccttag
     1501 ccgaccggtc catgataaca tcatggaact gctcattgtg tgcgatgcgt tgaggcgcgc
     1561 gtcggctcgc tccatcaccg cagtggtgcc atattacgca tatgcgcggc aggatcgcaa
     1621 ggcgcaaccc agggagccca ttgcggcgaa actcctggcc aacctgataa cggcggccgg
     1681 ggcgacgagg gtgctcacca tagacctaca cgctccgcag atccagggtt tcttcgatat
     1741 cccggtggac cacctgaagg cggcccccat acttgcgcag cacttggacg cacgcggact
     1801 gcgcggatgc gtgatagtat cgcctgatgt gggaggtgtc acccgagcga gggtgatcgc
     1861 tgagcatctt gacgcgaccc tggcgattgt ggacaagcgt cggccacggc ctaatgtatc
     1921 ggaagtcatg aacatcatag gtgaagtcaa gggcaaaccg tgcgtgatag tggacgacct
     1981 agtcgacacg gccgggacga tagtgcaggc tgctgcggca ttgctcgatg cgggagcgac
     2041 ggaggtcagg gcgtgctgca ctcatggagt gctttcgggt ccggcgatcg agcgtctgaa
     2101 atcatcggtt atccaagagc tggtgatcac aaacacgatt ccagtcagtg cccacgataa
     2161 tatcgacaag attacggttc tgtcggtggc ccccctgttt gccgaggcca tccggcggat
     2221 atacgaagag ctctcggtga gcatactctt tgagtagcag gccacagaat gcgcgctgca
     2281 gggcgggacg cgcactgcag gccgtaagct acaggcagca ggtattgaga tgtttctgat
     2341 aattgggctc gggaaccccg gatctcggta cgagtgcaca cgtcacaatg tggggttcat
     2401 ggtcctcgac cgccttgggc gcgaactggg agttgccttc cgaaaggcgc cttatcacag
     2461 cctcgcgact cgtgtcgggg tatccggaaa tgacttggtc ctcataaagc ctcagaccta
     2521 catgaacaac agcggcattg ctgttgccgg agcgacgcgg tggcacaaag tcagccccga
     2581 gtcgctaatc atcgtgtacg acgacatgga tctcgaagtg gggcggttgc gcatacgcgc
     2641 gaggggcagc gccggcggtc ataacgggat gaagtccgtc gttgagcatt tgggaaccga
     2701 agagatcgtc cggctgcggc tgggtattgg acgtcccgag ccgtgggatg accccgtaga
     2761 ctacgtgacc gggctatttc gcagggatga gcttcctgct atgtcggatg cgattatgtg
     2821 cgcggtccgt gccatactat gtattgtgga agagggcgtg gacgtcgcac agacccgctt
     2881 caattgaaca cgttcacata ggcgcgttcc cagccgaggt gcggctgggc catgctgcaa
     2941 ttccctcggt cgcctctcaa gttgccttgg aggtgtgggc ttgaccggga ttctgaacac
     3001 attgctcgaa tccgggacgt tctccgatgt gctgagcaga atagcccgcg gtgcaaacaa
     3061 ggctcagata gccggtctgt ccggctcgcg aaagtcgttg ttcatagcgg gtttgctttg
     3121 gcatactcga aggactgtgt ttgttgtgac tccgaaccag ctagaggcgg acgcgatcgc
     3181 gggcgacttg acggccctga ccggccaaga ccgcgtattt gtcttccctc ctgtcgacat
     3241 gatgccgtat gaagaggcgc ccctaagcgc ggatcttgca gccgcccgta caagagcgct
     3301 ggtgtcgctg atggaggtcg ggcgccaatg tgccattgtt gctcccgtgc gctctctggg
     3361 caagcggatc gttccgatct cggtgttccg agaacacgta ttccgtcttc gccagggcga
     3421 gacttgtgac ctggacgagc tggctgaccg tcttgtttcc ggcggttacg atattcatgc
     3481 gaaggtggaa tcgcgcggac aggccagtgt gcgcggcggc ataatcgacg tgtttccgca
     3541 cgacgccgat tcgcctgccc ggatagagct gttcggcgac gagatcgact cgataagatc
     3601 atttgacgtg aacacacagc ggtcgaccgg actcatggac gaggtgttga taaccccggc
     3661 ccgcgagttt ctctacgatt ctgagcggct gggccgtgcg tgtgatgcga tccgaggcga
     3721 actagagcgt gtgggcagtc cgcagctacg agagcgagtc atacaccatc tggagcgctt
     3781 tgagaatggc gctcgattcg acaatgaaga gcagtatctg ccgtttttct accccgagag
     3841 ggttgcgccg gtggattggg ttcccaatgc gctcttcatc atagacgagc ctgcgcgggt
     3901 ggaggaggcg ctcgatggat atgaggctga cgtgcgggag acttatgcga gcctgcttca
     3961 ggcgggcatg gtcctgccgt ctcaagcgga atcgtaccta agcgcccacg atatcgccgc
     4021 ttctatcgac aggaggcagg ccgtgtcgat gtcgatcctg tcgcgggaga taggggctac
     4081 ccctgggact actgctggga ccgggaccga ggatgcccag ttgatcgccc cagtcaagca
     4141 ggccgacctg tacgcgggcc gcttcgaaga tctggttcac gacatcaaga agtaccggaa
     4201 gcggcaatac cgtgttgtat gcacagtatc tacagcagat cgtcgcgata gactggccga
     4261 gaccctagaa gacagcggcg tgccggttac aaaacttgcg acccttagtg aagtgcctga
     4321 tcagggaagt gtcagcgtag tcacggcgga gatgacgtcg ggctttcagt atcccgactt
     4381 gaagcttctg ttgctcactg atgcggagat ctacggtagg cagaaaaaga ggcggctgtt
     4441 cgcggcggct gaagagggag ccaggatcgc cagctacacc gatctcactc ctggcgacta
     4501 tgtggtgcat gtcaatcatg gagtaggtcg gtattctgga gttcaaaggc tcgaagtggg
     4561 tggagtgcag cgcgactact tgctggtgca gtatgcgggc gaggatcggg tgtacgtgcc
     4621 caccgaccag gtagccatgc tccaaaaata catcggcatg gaggatgcag ccccacggct
     4681 gaacaagctg ggcggcggcg aatggacgcg ggccaagaac cgagtcaggg agtccgctcg
     4741 ggacatggct cagaacttga taaagctgta cgctgcgcgc ggtagcgtga aaggttactc
     4801 cttctcgcgt gacactgtgt ggcagcagga attcgaggac gcttttcagt atgaagagac
     4861 tccggatcag tggcgtgcca tcgtagatgt caagcgcgat atggagaaag cgctgcccat
     4921 ggataggctg ctctgtggcg atgtgggctt tgggaagacc gaggtggctc tgcgcgcggc
     4981 tttcaaggcc gcggccgacg ggaaacaggt ggcggtgttg acgcccacga caatcttggc
     5041 ccagcagcac tacaacactt ttactgagcg tttccggggt tttcctatga gaatagcgat
     5101 gctgtcgagg ttccagaccg aagctgagca acgcgaaata gtgcggggca tcaagctggg
     5161 ttcggtggat gtagtgatcg gaacgcatcg actgctgtcg caggacatag ccttccgtga
     5221 tctgggcctt ctcgtggtag acgaagaaca gcgattcggc gtcgtgcaga aggagcggat
     5281 caaggaactc cgcaaggaag tggatgtcct taccttgacg gcaacgccca ttccgaggac
     5341 attgcacatg gctatggttg gcgtccgcga catgagtctc atcgagactc ctcccgaaaa
     5401 caggtttcct atcagaactt atgtactgga acagaatgag accctgataa gagaggctgt
     5461 gctcagggaa gtggatcggg gcgggcaggt ctacttcgtg cacaacaggg ttcagactat
     5521 cgacgaggcg gcggcggtgc tcgcacggct cgtacctgag gtctccatag gcgtggccca
     5581 cgggcagatg cctgaagacc gactggagcg agttatgctg gattttctcg cgagtgagtt
     5641 tgatgttctc gtctgtacag ctataataga atctggtatc gatattccta acgtcaacac
     5701 cattgtgatc aacgacgctc agaattttgg actggcccag ctttaccagc tccgcggtcg
     5761 agtgggccgc acgaataggg tagcctatgc ctaccttctc ttcaggcggg acttggttct
     5821 aactgagctt gcggagaaga gactcgcagc catacgtgaa ttcacgagcc tgggatcggg
     5881 ctacaagata gctttgcgcg acctggaaat ccgaggagcc ggtaacatac tcggtgctga
     5941 acagcatggg cacatagcgg ccgtcggttt cgagatgtac tgccgacttc tggaagaaga
     6001 gattagggag ttgaagggcg aggcgaagag acctgaaccc gctcaggtga tgatcgatct
     6061 gccagtcgat gccttcgtac cggatagcta tgtgtccgac tcgcggcaga agatcgatgt
     6121 gtacaagaag atcaatggcg tcgataccat ggaagatgca gctgacctca cacgtgagtt
     6181 tgtcgacaga ttcggccc
//
LOCUS       DAOTAC010000148         6136 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_10818_length_6136_cov_5.060516, whole genome shotgun sequence.
ACCESSION   DAOTAC010000148 DAOTAC010000000
VERSION     DAOTAC010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6136
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10818_length_6136_cov_5.060516"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="PLM74_07285"
     CDS             complement(<1..245)
                     /locus_tag="PLM74_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-5'-phosphate-dependent protein subunit
                     beta"
                     /protein_id="HON42912.1"
                     /translation="MSRRMVWGPTFEEMLHPDKIDPAIRAKALAAKESDPLNEINLYN
                     ITWKRPDGKPCYIVLPKELTGVDANIIALYGRDFPTGS"
     gene            complement(457..2280)
                     /locus_tag="PLM74_07290"
     CDS             complement(457..2280)
                     /locus_tag="PLM74_07290"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HON42913.1"
                     /translation="MPAEKRGLASIAKDAQKVCDAIAAVIGLEVEVVDKSLLRIAGTG
                     KYRLRVGHSLEHEGAAYRQVLRTGQGTIVTRPGMEPICGLCPKYGSCDEKAELSAPIT
                     VEGEVVGVIGLLCFDESQRATLLSRLESHKDFIEHMAGLLASKVREHALLEQERRAAQ
                     EVAALLDQMTDPVLAVDQNGKVIHCNVPAARLLNLPRGDGLSGDTRGFDASVGAEGLE
                     FLERVLKTRAGFDDKEMFLSFGGVEKPYLVSATPLAYGGLIYGAMAVIRPLGELHRWA
                     YNLTNAVKVFSLNDIIGESPAIQLVKEKALKVAGSKSSILITGESGTGKELLARGIHN
                     ASARADGPFVAVNCAAIPDTLLESELFGYEEGAFTGARKGGKPGKFELADGGTIFLDE
                     IAEMSIHLQAKLLRVLEGWEVERVGGTRPRPVDVRLMSATNQDLTEKIACREFREDLY
                     FRLNVIPLRVPPLRDRAGDIPLLAMHFVRKHAALLGKEIERIDPEVAELLETYEWPGN
                     VRELENAIEYAVNLEGTSTIHERSLPETIRARASSLPKGSCDSGGIDDMEARLIVEAL
                     SRHGYNYSGKQEAARELGIGIATLYRKMARYGIPGVRGARP"
     gene            complement(2387..3694)
                     /locus_tag="PLM74_07295"
     CDS             complement(2387..3694)
                     /locus_tag="PLM74_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HON42914.1"
                     /translation="MAANTPEWIRSAIIYEVFPRNHTAEGTLAGVYRDLERIASLHTD
                     VLWLMPIHPIGVVGRKGPLGSPYAIDDYRAIHPELGDERGLRMLIDKAHALGMKVMID
                     VVYNHTSRDSVLLSEHPEWFMHDVHGKVAGKVGDWSDVYDLDYSHRSLWEYQIATLQK
                     WADCGVDGFRCDVASLVPLDFWVAARERLAQGESREVIWLAESVDKSFTKLLRDEGFV
                     SHADPELHTAFDLTYDYDGYEYLRGAMQGARPLGDYLNYLYIQETLYPAGAVKMRFIE
                     NHDNPRAAGIVGSRHSLLNWTVFYSLLPGATLVYAGQEVALSHCPDLFDKDTVRWDEG
                     DPEFQPFMQKVLGLSKEIKKSCTRFAVSELREGVAKIVWRGTGAIYVALVNLSGKDGE
                     ICLNEREATELRYGEVCSGRLDECGRLRLRLEDMPLVIRDISF"
     gene            complement(3840..4397)
                     /locus_tag="PLM74_07300"
     CDS             complement(3840..4397)
                     /locus_tag="PLM74_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42915.1"
                     /translation="MRMNAVRTLALVSVVLVFLFGFGLVGCAAEGPAPVCPQPEPVCR
                     CEPITVIVGCGECTRCDEREIQLCPPVRMPQTPSIVKNLVIDLVQVQNGRVVVFAHVD
                     KLITYIDVNGVTRTRLERVPFACEIPIEGIVFTDTVAFQSIVITEETDTLCSDGRTLR
                     ERLCVRINVSIQRIIGCRLVCEPDF"
     gene            complement(4493..4918)
                     /locus_tag="PLM74_07305"
     CDS             complement(4493..4918)
                     /locus_tag="PLM74_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42916.1"
                     /translation="MDMPGPLDIPPGKMVIFAVETAEDAIVFEVALDPPGAQILDVCD
                     HAGVVTIELGEDAAVATVNVGATLLYADSEGEEHTTMYDKATLLRIDMPGVLSTDCVA
                     GHILDCLGIDDQLVDEGRIWRHTRRVSATVSAERAYWHM"
     gene            complement(5140..5619)
                     /locus_tag="PLM74_07310"
     CDS             complement(5140..5619)
                     /locus_tag="PLM74_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HON42917.1"
                     /translation="MKIPVSLKHKPVIVVEDYEHVDGRRAYDSDAKGLSLGLAQWNDR
                     GKVEISAKVWRHTGEKWSRQSEELPLHRALDLALLICKARQHFMEAYRYEKLYDPDMT
                     TIGRVGLQGDAMTVKVCTENPMIDEDIKLFRDCISRDDELIGERLTALSAILKDMGY"
BASE COUNT         1205 a         1921 c         1619 g         1391 t
ORIGIN      
        1 gaaccggtgg gaaagtcccg gccatacagc gcaatgatgt tcgcgtcaac gccggtcagc
       61 tctttgggaa gcactatata gcacggtttc ccatcaggcc gcttccaggt gatgttgtag
      121 aggttgatct cgttaagcgg atcgctctct ttggcggcga gagcttttgc ccggatcgcg
      181 ggatcgatct tgtcgggatg aagcatctct tcaaacgtcg gtccccaaac catcctccga
      241 gacattcaat ttcctcccat tcatattgta cactgcgcct gcatactcac actcacgata
      301 catgcaagtt ccgcgccgag cgacgctaaa agcgcgaatc tgatgacaac tcgcccttta
      361 gctggcctgt tattcgcttc ggagcgacgc agccggtgtc ggttgatctt ggttgagagg
      421 tgttttctat cgatatgata attggggtga tagttatcat ggcctcgcgc cgcgaactcc
      481 cgggatgccg taccgggcca tcttgcgata gagtgtggcg attccgatcc cgagttcgcg
      541 cgcagcctcc tgcttgccgc tatagttgta cccgtgccga ctcagcgctt ctactatcag
      601 acgtgcttcc atgtcgtcga tgcccccaga gtcgcacgac cccttcggga gactcgacgc
      661 tctagcacga atcgtctccg gcaggctccg ctcatgtata gttgatgtgc cttccagatt
      721 gaccgcatac tctatggcgt tctcaagctc gcgcacgttg ccgggccact cgtaggtctc
      781 aagcagttcg gcgacttcag gatcgattcg ctcaatctcc ttgcccagga gggccgcatg
      841 ctttcgaacg aaatgcatgg ccagcagcgg gatgtcgcct gccctgtctc gaagaggcgg
      901 cactcgcagc gggatcacat tcagccggaa atacaggtct tcgcgaaact ccctgcaggc
      961 aatcttctcg gtcaagtcct ggttggttgc ggacataagc cgtacatcca cagggcgcgg
     1021 ccgggtgcct ccgacccgct ctacttccca gccttcaagt actctgagca gtttcgcctg
     1081 aaggtgaatg gacatctcgg ctatctcatc caggaagatc gtgcctccat cggcgagttc
     1141 gaacttcccg ggtttcccgc cttttcgtgc accggtgaat gctccttcct catacccgaa
     1201 aagctcgctc tcaaggagcg tgtcgggaat cgcggcgcag ttcaccgcca caaatggccc
     1261 gtcagcccga gcacttgcgt tgtgtatccc ccttgccagc agctcttttc cggtaccgct
     1321 ctcccccgta atcaggatgc tggacttgct ccctgccact ttgagggcct tttccttgac
     1381 cagctgaatc gcaggactct cgccgataat gtcgttcagg ctgaacactt tgacggcatt
     1441 agttaggtta tatgcccatc ggtggagttc ccccagggga cggatgaccg ccatggcgcc
     1501 gtagatgagc cccccatacg ccagcggtgt agccgacaca agatagggtt tctccactcc
     1561 cccgaagctg aggaacatct ccttatcatc gaaccccgcc ctggtcttca gaacgcgctc
     1621 cagaaactcc agtccttcag cgcctaccga cgcatcgaat ccgcgtgtgt cgccgcttaa
     1681 gccatcgccc ctgggaagat tgagcagacg ggccgctgga acgttgcagt ggatcacctt
     1741 tccattttgg tctacagcaa gcaccggatc ggtcatctga tcgagcaagg ccgctacctc
     1801 ctgagctgca cgccgctcct gctccaagag ggcgtgttct cgcaccttac ttgcaagcag
     1861 gccggccatg tgctcaatga agtccttgtg gctttcgagc ctcgaaagca gcgttgcgcg
     1921 ctgggattca tcgaaacaca gtagtccgat tacgcccaca acttcgccct caacagttat
     1981 cggcgccgag agctccgcct tctcatcgca acttccgtat ttcgggcaca agccgcagat
     2041 gggctccatg cccggcctgg tcacgattgt gccctgacca gtccggagga cttgccgata
     2101 agctgcaccc tcgtgctcga gtgaatgccc gacgcgaagg cggtacttgc cggtgccggc
     2161 tatcctgagc aggctcttgt cgactacctc aacttctagc cctattactg ccgctatggc
     2221 gtcacatacc ttctgcgcat ctttagcaat gctagcgagt ccacgtttct ccgcgggcat
     2281 gcctcatacc cccgccgagc acgctcgcac gcgtgcataa tagattggat gcagaatatt
     2341 gagtgcagta tagtagtggt ttctctaccc attgccgttc tccttctcaa aatgatatgt
     2401 ctcttatcac caacggcatg tcctccaggc ggaggcgcag gcgcccacat tcgtccagcc
     2461 tacctgagca cacctcgccg taccgcagct cggtcgcttc gcgctcgttt aggcagatct
     2521 cgccgtcttt gccgctcaga ttgacgaggg caacataaat cgctccggtt ccgcgccaca
     2581 cgatcttagc cacgccttcg cgcagttcgg acacggcaaa ccgggtgcag gacttcttga
     2641 tttccttgga tagccccagg accttctgca tgaacggctg aaactcagga tcgccctcgt
     2701 cccagcgtac cgtgtctttg tcgaacaggt ctgggcagtg actcaaagcc acttcctgcc
     2761 ctgcatacac cagcgtcgcc ccgggaagca gggagtagaa aaccgtccag ttaagcaggg
     2821 aatgcctgct gcccacgatg cccgcggcgc gcgggttgtc gtggttttcg atgaagcgca
     2881 tcttcacggc gcccgcagga taaagagtct cctgtatata taggtagttg agatagtcgc
     2941 cgagcggcct tgcgccctgc atggctcctc tgagatactc gtacccatcg taatcgtaag
     3001 tcaggtcgaa ggccgtgtgc agctcggggt cggcatggct gacgaacccc tcgtcgcgaa
     3061 ggagctttgt gaaagacttg tccacactct ccgcaagcca aatcacctcc ctggactcgc
     3121 cctgcgcaag ccgctcgcgg gcggccaccc agaagtccag cggcaccagg gacgcaacat
     3181 cgcagcggaa tccgtctacg ccgcaatcgg cccatttctg cagcgtggca atctgatact
     3241 cccagaggct tctgtggctg tagtctaggt catagacgtc cgaccagtcg ccgaccttgc
     3301 ctgcgacctt gccgtgcacg tcgtgcatga accactcggg gtgttcgctg aggagcacac
     3361 tatcacggga tgtgtgattg tacaccacat cgatcatgac cttcatccca agagcgtgcg
     3421 ctttgtcgat cagcatccgg agaccgcgtt catcgcccag ttcggggtgg atcgcccggt
     3481 agtcgtcgat agcatatggg ctgccgaggg gccctttgcg cccgacgacg cctattggat
     3541 gaatgggcat cagccacagc acgtcagtat gtaagctggc tatccgctca agatcgcggt
     3601 agactcctgc aagtgttcct tcggccgtat gattcctcgg gaagacctca tagatgatgg
     3661 cgctccttat ccactccgga gtattggcag ccattgtgat gacctcctag gattggcatg
     3721 acatgtttac tttatcatgt acgtcggcag ggcgtaaaca ccgccagccc ggcgccgaac
     3781 aaggtcgggc cgggctgtgc cgggctaggc cgtattgtgc tgtgctgagt gcgtgtgccc
     3841 tagaagtcgg gctcacacac caacctgcac ccgatgatgc gctgtatgga tacgttgatc
     3901 ctgacgcaca gtctctcgcg cagagtccta ccgtcgctgc acagagtgtc cgtctcctct
     3961 gtgatgacga tgctctggaa tgccaccgtg tcggtgaaca ctattccctc gatcggaatc
     4021 tcgcacgcaa acgggaccct ttccagcctt gttctggtga ctccgttgac gtcgatgtag
     4081 gttatgagct tatcaacgtg ggcgaacacg accaccctgc cgttctgtac ctgaacaagg
     4141 tcgatcacaa ggttcttgac aatcgaagga gtctggggca ttctgacggg cgggcacaac
     4201 tggatttccc gttcgtcgca ccgagtacac tccccgcagc cgacgatcac cgttatgggt
     4261 tcacatctgc ataccggttc cggctgcggg cacacaggcg ccggtccctc ggccgcgcag
     4321 cccacaaggc caaagccgaa caggaacacc agaacgacgc tcacgagagc aagggtgcga
     4381 accgcattca tgcgcatttg agcttcctcc tccatgatgg gattgctcta cacgctatgc
     4441 ggacacgcca cgactgcaac cgagctagag gcataatagg acaaccggca ggtcacatgt
     4501 gccagtatgc tcgttcagcc gacacagtag cgctcacacg gcgcgtgtgc cgccatatcc
     4561 tcccttcgtc taccagttga tcgtcaatgc caaggcagtc aagaatgtgc cctgccacgc
     4621 agtctgtgct caacaccccg ggcatgtcta tccggagcaa cgtggcctta tcatacattg
     4681 tggtatgctc ctcgccctcg ctgtcggcgt aaagtaacgt cgcccccaca ttaacagtgg
     4741 caacggctgc gtcctccccc agttcaatcg tcacaacgcc ggcatggtcg caaacatcca
     4801 gaatctgtgc gcccggcgga tccaaagcga cttcgaacac aatggcgtcc tcagcggtct
     4861 caaccgcgaa gattaccatc ttcccaggcg gtatatccag cgggccgggc atatccatgc
     4921 cgtctcacct cctcgctccc ctattctctt gcgcttcctt ccgcgcacaa tcttaagcgc
     4981 aggcttcggc gcaatctccg atcagcttac gcgccgacaa gcggtatgga cactctgttc
     5041 caatggggag ccgcttcgtc agatactgtc cgctgttttc agctggcgtt ttctttcgct
     5101 cgatatgccc atggtgaatc cctccatcgg ttccgagcat caatacccca tgtccttcag
     5161 aatcgcagaa agcgctgtga gcctctcccc gattaactcg tcgtccctgc ttatgcagtc
     5221 tctaaacagc ttgatgtcct cgtcgatcat ggggttttcg gtacacacct taacagtcat
     5281 ggcatcgccc tgcaggccta cccggccaat ggtggtcatg tcggggtcat agagcttctc
     5341 gtagcgatac gcctccatga agtgttggcg ggctttgcag ataaggagcg ccagatcgag
     5401 cgccctgtgc aggggcagtt cctccgattg cctggaccat ttctccccgg tatgccgcca
     5461 tacctttgct gatatctcca ccttgcctcg gtcattccat tgggcaagac ccaacgacag
     5521 acctttggcg tccgagtcat acgcacgcct gccgtccaca tgctcatagt cctcaaccac
     5581 gatcacgggc ttgtgcttaa gcgaaaccgg aatcttcaat gcgcgcacct catctcatgc
     5641 tttaggttag tagtttactc gtttactgct taagtcaaac ccgcacggcg aggaatgtca
     5701 agatggtgcc cggggcctgg ggcgcatggc acggcgctca cggcgaggcg cgagtaggga
     5761 ttgtaggcaa cagcagcggg cctattgccg gttcgggact atcggctccc agggctcgag
     5821 ttccagcctg tcgtgtggtc gctatccatt acccggatcg catttggccc aaccacattc
     5881 tggctcatcc tattcgtttt tcggcaggtt ttttgatcaa cctcctcgga tcctgggtgg
     5941 tttggcaggt acgagttatc tgaattcgcg tcggcaacgg cgcccccttt cttcggagcc
     6001 acgccggcaa caaatccgca ggcataggcg ggtaacataa tatggatacg cgtgtcgggg
     6061 ttctcgagcg cgccggaaaa ggccctcagg cagcccttga tctactaaga aaacccatat
     6121 tggtccggcc ctcttc
//
LOCUS       DAOTAC010000149         6097 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_10909_length_6097_cov_4.781695, whole genome shotgun sequence.
ACCESSION   DAOTAC010000149 DAOTAC010000000
VERSION     DAOTAC010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6097
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10909_length_6097_cov_4.781695"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1075)
                     /locus_tag="PLM74_07315"
     CDS             complement(<1..1075)
                     /locus_tag="PLM74_07315"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HON42918.1"
                     /translation="MSVLSKRLLTAVSIVLVVAVTASAAIAAPAVVTDVPKDHWAYEA
                     VTLLVNKGYMGVYESGQFRGDDNVSRYLLAFVAARLLRDVESGRTTLTDDDMRVVREL
                     SVNLREQLAVVTGRLSELEKAVADAAGDAAIAREDVARGAAGTKVLQADLADKTAAIE
                     EQIKQIKNDAAARAGASDQKLTALYADSKQQMAAGDEAIRQEVAQQLAVVSAKTDSVQ
                     ASALAAVAGEAKSRQDLESRLSLSIDGLKSADEAQKAQIGQLSLKLEDAVAAIASARA
                     WAEAAIAGEAERNNVLHAELASKTAAIEEQLKQIQDDAAARAGASDQKLTALYADSKQ
                     QMALGDEAIRQEVAQQLAVVSAKT"
     gene            complement(1104..3125)
                     /locus_tag="PLM74_07320"
     CDS             complement(1104..3125)
                     /locus_tag="PLM74_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulosome anchor protein"
                     /protein_id="HON42919.1"
                     /translation="MSSAINERRRARRFTAAIAAILCTLCACGLGAGAASPTPVQMSN
                     DFIKIVVNPGPEEAGRFGVETTGGDPRNPADDDLPLIYGRPLPWTSYTTVQVDGVDWV
                     FGGKTTRRAGRTGRYGDVVGPVEVRDGRVVCSYRLGDVKVTQQLSFVKSPTTRYLDTA
                     RIHYIVENVGQMTHAVGLRLLIDTMLGANDGAPLRAADQAMIGDSMLSGAGIPDYWQA
                     FDSLVEPSVTSQGTMAGGELTPPSRVAFSNWGTFADNLWDAEIVPGREFIRSRESDLD
                     SAVAMYWDPSALAPGEVRHCVTYYGLGGITLAPGKLTLGITAPAEVGLSSDGTAPFLA
                     VAYVHNTGEGPAHDLVARLDLPLGCKLAGGQSNTRSIGDLEPGQTAQVAWQVIATWAS
                     VGRADLVVSVSAKNADPNGVSREIQILGPPRLLASLRRVEALQVREERLYPWPARVTV
                     RVENTGGSDANWVRVRLDSLGDFALSGGDHRELLLGSLQPNQGQDISWMLGGEPRPGE
                     QRIVASATAQNAAPSASSASIQVPELRPRVWLRPEKERVKAGDIVRVDVMLTNIVGVD
                     SFKFDLSYDRALLGLAMVSRGGLAVAGKTMLPWEEGDSRSVPGRILGMAGRVGEPGKV
                     WGSVATVYFLSGQYGSAVLSIEDLILLDSSGRAITLPEAPVPCTVQISR"
     gene            complement(3165..4130)
                     /locus_tag="PLM74_07325"
     CDS             complement(3165..4130)
                     /locus_tag="PLM74_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42920.1"
                     /translation="MSGRERERGSLESLLISIAVHLAVILLVPYVEQPILDVYPLDIA
                     GVIEITTIEAAPAAPAPAPGVVVQEVAQRPKPEAPEPAKPEPVKPTPAPAPKVTPKPE
                     PKVAAVPEPTRQEPPKQEKPEVVTSPAGRVPSPVVAKDPPESKLPEPKPTPEPTPELT
                     PEPTPEAVQKQTPSPAAESPATQVAEVTRSPGAGQPDGVGTIPRPEYPGDDPTGRSMM
                     LSTGGGDHGVWIPKGVQNLRGRGEAMVRIRVRADGSIESISFLESPPDPAMTQAIRSA
                     ITTAWRFAKDSTSGRAESYYLDVRVGYDGGTGDIWVKSERASYIR"
     gene            complement(4127..4552)
                     /locus_tag="PLM74_07330"
     CDS             complement(4127..4552)
                     /locus_tag="PLM74_07330"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HON42921.1"
                     /translation="MFGKYRKRRRTRVEILPMIDIMFYLVLFFMVFGTFRTETAGVPL
                     ELPRAATPSDLTRDHIIVAIDSKGKLYYNERQFADGDLTRALLPQLRANPSRVVIVKA
                     DRSVSYERLIQTIDAIRMAGGVHLALAVERAAGSGGGTR"
     gene            complement(4533..5171)
                     /locus_tag="PLM74_07335"
     CDS             complement(4533..5171)
                     /locus_tag="PLM74_07335"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HON42922.1"
                     /translation="MLKYISAGGPVMYPIIVCSVVGMAVSIQKIIQLVLMPTGSDQLM
                     RSVKVNMSQGRPVEAMQAAKAAKGPVAGLARAAVSNYGRPYNEIKDAIEMAGREEVHA
                     MERKMNVLDGIVTLAPLLGLLGTVTGLIKNFQVLSAFAGLTTPAQLSGGIAEAMITTA
                     AGLIVAAPLMLVYSWLSVIIDDKVAEMDRRAAELLAITAGDGGGRGDVREIS"
     gene            complement(5265..>6097)
                     /locus_tag="PLM74_07340"
     CDS             complement(5265..>6097)
                     /locus_tag="PLM74_07340"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HON42923.1"
                     /translation="QAVGAQEEAVVKTLTPEEALKIVPFLNIDGMVGATFCQEDGHAN
                     PFHTTFAYARAARRLGVEILTHTEVVGIEVENDKIRAVVTDRGRINTSTVVNCAGAYS
                     GHIAAMAGIDLPVWGERHQILITEPVEPVMGPMVISFHHDFYCQQVPHGSFIMGIGMP
                     GEPRGYRIHSSVDFLEGMARSLRQLLPPVAQLRVVRQWAGLYDRSPDAQPILGECPPV
                     ERFFNANGFSGHGFMLAPIVGVIMAELITTGSSSIDVSKLDAGRFARGELVLEPAVVG
                     "
BASE COUNT         1137 a         2020 c         1723 g         1217 t
ORIGIN      
        1 cggtcttcgc cgacaccacg gccagttgct gggcgacttc ctgtcggatc gcttcgtctc
       61 ccagagccat ctgctgcttg gagtcggcgt acagcgcagt tagcttctga tcgctggcgc
      121 cggcgcgagc tgcagcgtca tcctggatct gtttgagttg ctcctctatt gccgctgttt
      181 tggaagccag ctctgcatgc aacacgttgt tgcgctcagc ttcgccggca atcgcagcct
      241 cagcccatgc cctggcagat gcgatggccg ccactgcatc ctctagcttc agcgacagct
      301 ggcctatctg cgccttctgc gcctcatccg cgctcttcag cccatcaatc gaaaggctca
      361 accttgactc aagatcctgg cgggacttgg cttcgcccgc tacagcagcc agcgcggagg
      421 cctgcaccga atcggtcttc gccgacacca cggccagttg ctgggcgact tcctgtcgga
      481 tcgcttcatc tcccgcagcc atctgctgct tggagtcggc gtacagtgca gtcagcttct
      541 gatcgctggc gccggcgcga gccgcagcat cgttcttgat ctgtttgatc tgctcttcta
      601 ttgccgctgt cttgtcagcc aggtcagcct gtagcacctt cgttcccgct gccccacgcg
      661 cgacatcctc cctggcgatg gcagcgtcac ctgctgcatc ggcaaccgcc ttctcaagct
      721 cactgaggcg gccggtgacc accgccagct gttctctgag gttgacagag agctcacgca
      781 caactctcat atcatcatcg gtgagcgtgg tccggcccga ctccacgtcg cgcagaagcc
      841 gcgccgccac aaacgccagc aggtacctgc tcacgttgtc gtcccctcgg aactggcccg
      901 actcgtaaac acccatgtat cccttattca ccagcaatgt cacggcttca tatgcccagt
      961 ggtccttggg cacatcagtg accacagcag gcgctgctat ggcggccgag gccgtcacgg
     1021 cgactacgag cacgatactg actgcggtga gcaaccgctt tgatagcaca gacatcccta
     1081 tccctccttg cctttcgact cttttacctg ctgatctgca ccgtacaggg caccggcgcc
     1141 tcggggagcg tgattgccct gcctgatgaa tccagaagaa tcagatcctc tatagacagg
     1201 actgcgctgc catactgccc tgacaggaag taaaccgtcg cgacgcttcc ccagacctta
     1261 ccgggctcgc cgacacggcc ggccatgccc aggatacgtc ctggcactga tcgcgagtcg
     1321 ccctcctccc acggcaacat ggtcttcccc gccactgcaa ggccgcccct agataccatg
     1381 gccagcccta gcagagccct atcgtagctt aggtcgaact tgaatgaatc gacacctacg
     1441 atgttcgtga gcattacgtc cacgcgcacg atgtcgcctg ccttgacgcg ttccttctcg
     1501 ggcctgagcc acaccctcgg cctgagctcc ggcacttgta tggatgcgct cgacgccgac
     1561 ggtgccgcat tctgggcagt ggcagacgca actatcctct gctcacccgg cctcggttcg
     1621 ccgcccagca tccatgagat gtcttgccct tggttgggct gcaagctgcc gagcaagagc
     1681 tcacgatggt ctccgcctga cagggcgaaa tcgcccagtg aatcaaggcg aacgcgaacc
     1741 cagttggcgt cggatccgcc ggtgttctcc actctcacag tcaccctggc cggccacgga
     1801 tagagtctct cttcacgaac ctgaagcgcc tccacgcgcc tgaggctagc cagcagtcgt
     1861 gggggcccaa ggatctgaat ctccctggac actccattgg gatctgcgtt cttggcagat
     1921 accgatacaa cgagatcagc ccgtcccaca gacgcccagg tggcgatgac ttgccacgcg
     1981 acctgggccg tctgcccagg ctctaggtcg ccgattgagc gcgtattgct ctggccgccc
     2041 gcaagtttgc agcccagggg caggtctagc cgtgcaacaa ggtcatgagc gggcccttcg
     2101 ccggtgttgt gcacgtaagc cacggccagg aagggtgccg ttccgtcaga cgacaggccg
     2161 acctctgcag gcgccgtgat gcccagagtg agtttgccgg gagcgagtgt aatgccgccc
     2221 agaccgtagt aggtcacgca gtgcctgacc tctcccgggg ccaacgcaga tgggtcccaa
     2281 tacatcgcaa ccgccgaatc cagatcggat tcgcgcgaac ggatgaactc gcgtccgggc
     2341 acgatttcgg cgtcccacag gttgtcggca aacgtgcccc agttggaaaa ggccacgcgc
     2401 gacggcggcg tcagctcgcc gcctgccatc gtgccctggg acgtcactga aggttcgacc
     2461 agcgagtcga aggcctgcca gtagtcgggt attcccgccc cggaaagcat cgagtcgcct
     2521 atcattgcct gatctgcggc gcgcaacggg gcgccgtcgt ttgcaccgag cattgtatcg
     2581 atcagcaggc gcaatcccac ggcgtgcgtc atctggccca cgttctccac aatgtagtga
     2641 atcctcgcag tatccaggta gcgtgtcgta gggctcttga cgaacgagag ctgctgggtg
     2701 actttgacat cacccaggcg atatgaacag accacgcggc catccctgac ctccacgggc
     2761 ccaactacat ccccgtaacg gcctgtcctg ccggcgcgcc tggtggtctt gcctccaaat
     2821 acccagtcca ccccgtctac ctgcacagtt gtgtaggacg tccatggcag cggtcgccca
     2881 tatatgagcg gcaggtcatc gtctgccgga ttgcgcggat ctcctccggt agtctctacg
     2941 ccgaaccgcc ccgcctcctc agggcctggg ttgaccacaa tcttgatgaa atcattgctc
     3001 atctggacgg gtgtggggct cgcggccccc gcgcccaggc cacacgcgca gagggtgcac
     3061 agaatggcgg cgatggctgc ggtaaagcgg cgcgcccggc gccgctcgtt gattgctgaa
     3121 gacaagctag acacctcctc gtgccggcga tgatcagaac ccggttaccg tatgtacgac
     3181 gcgcgctcgc tcttgaccca gatgtccccg gtgccgccgt cgtagcccac ccgcacatcg
     3241 aggtagtagg actcagcgcg acctgaggtg gaatctttcg cgaaccgcca ggcggtagtg
     3301 atagcactcc gtatagcctg ggtcatggcg ggatcaggcg gcgactccag aaaggatatg
     3361 gattcaatgg acccgtctgc acggacccgg attcgcacca tagcctcgcc gcggcctctc
     3421 aagttctgaa cgcccttagg aatccagact ccgtgatcgc cgccgcctgt ggacagcatc
     3481 atgctgcgcc cagtaggatc gtcgccgggg tactcaggtc ggggaatcgt acctacccca
     3541 tcgggttgcc cagccccggg cgatctcgtc acttccgcaa cctgtgtcgc cggcgactcc
     3601 gcggcaggcg acggcgtctg tttctggaca gcttctggag ttggttctgg agtcagttca
     3661 ggagtcggtt ccggagtcgg tttgggctcc ggcaatttcg attccggagg gtccttagcg
     3721 acgaccggag aagggaccct ccccgccggg ctcgtcacga cttcaggctt ctcctgtttc
     3781 ggcggttcct gcctggtcgg ttcgggcact gccgcaacct tgggctcggg cttcggcgta
     3841 actttgggcg ccggcgccgg ggttggcttc acaggctccg gcttggcagg ctcaggcgcc
     3901 tcgggtttgg gcctctgggc cacttcctgc acaacgactc ccggagcagg cgccggcgcg
     3961 gcaggcgcgg cctcaatcgt cgtgatttcg atcacacctg cgatgtcgag agggtacaca
     4021 tccaaaatgg gctgctccac ataaggaacc agcagtatca ccgcaaggtg aaccgcaatc
     4081 gatatgagca ggctctcaag gctgccccgc tctcgttcac ggccgctcat ctcgtgccac
     4141 cgcccgaccc ggcggcacgc tccaccgcca gggccagatg aacgccgccg gccatcctga
     4201 tcgcatctat agtctggata agccgctcgt agctgaccga tctgtcggct ttcactatca
     4261 caacacggct gggattagcc cgaagctgtg gaagcagagc tcgcgtgaga tccccatcag
     4321 caaactgcct ctcgttgtag tacagcttgc ctttagagtc gatggcgact atgatgtgat
     4381 cgcgcgtcag atcgctcggg gtcgcagccc gtggcagctc aagagggact ccggcagtct
     4441 cggtcctaaa agtgccgaag accatgaaga acaagaccag atagaacatg atgtctatca
     4501 tgggaagaat ctcgacccgc gttctcctgc gcttacgata tttcccgaac atcgccgcgc
     4561 cctcccccat cgccggccgt gatcgcgagg agttccgccg cccgcctgtc catctcagcc
     4621 accttgtcgt caatgatcac cgaaagccag ctgtatacca gcatgagcgg agcggcgact
     4681 atgagaccgg ctgcggtggt tatcatcgcc tcggcgatcc ctcctgacag ctgggccggc
     4741 gtagtgagcc cggcgaacgc cgacagaacc tgaaagttct tgatcaagcc cgtgactgta
     4801 ccaagaaggc cgagaagagg cgcaagagtg acgatgccgt ctaggacgtt catcttcctc
     4861 tccatcgcgt gtacctcttc tcggcctgcc atctcaatgg catctttgat ctcattgtac
     4921 ggcctgccgt agttggatac ggcagcacgg gccagcccgg ccacagggcc tttggcagcc
     4981 ttagcggcct gcatggcttc taccgggcgt ccttgagaca tgttgacctt cacagagcgc
     5041 atcagctggt ccgatccggt gggcatcaat acgagttgga tgatcttctg tatcgagacg
     5101 gccatcccca cgactgaaca gactatgatg gggtacatga ccggcccacc agcggatatg
     5161 tacttcagca ttggagcatt tcctccttaa cctgcatctg cgccctctac atccctttgg
     5221 acgcaccccc attcttctac gtattgcgcg aaattcctct aggtttatcc gactaccgca
     5281 ggttccagca ccagctcacc gcgggcaaat ctgccagcat caagcttact aacatctatg
     5341 gaactgctcc cagttgtgat cagctcggcc atgatcactc ccacgatcgg agcgagcatg
     5401 aaaccgtggc ccgagaaacc gttggcgttg aagaatctct ccacaggcgg gcactcaccc
     5461 aggatgggct gggcatcggg cgacctgtcg taaagccccg cccactgacg caccacgcgc
     5521 agctgcgcca caggtgggag cagctggcgc aagctccggg ccatgccctc caggaaatcg
     5581 actgaggaat ggatcctgta gcctctgggc tccccgggca tgccgattcc catgatgaaa
     5641 ctgccgtgag gcacctgctg gcagtagaag tcgtgatgga acgaaatcac catcggcccc
     5701 atgaccggct caaccggctc agtgatgaga atctggtgcc gctcgcccca caccggcaga
     5761 tcgatgccgg ccatggcggc tatatgcccc gagtacgccc cggcgcagtt gaccactgta
     5821 gacgtgttga tccgaccgcg atcagtcacc acggctcgga tcttgtcgtt ctctacctcg
     5881 atacctacaa cctccgtgtg cgtcagtatc tccacaccca gacgacgggc cgcacgcgcg
     5941 taggcaaacg tggtatggaa cgggttggca tgcccatcct cctgacagaa tgtggcccca
     6001 accatcccgt cgatgttcag gaagggcact atcttgagag cctcctcggg ggtaagggtc
     6061 ttcacaacag cttcttcctg ggcgccaacg gcctggg
//
LOCUS       DAOTAC010000150         6092 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_10917_length_6092_cov_4.810171, whole genome shotgun sequence.
ACCESSION   DAOTAC010000150 DAOTAC010000000
VERSION     DAOTAC010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6092
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10917_length_6092_cov_4.810171"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..877)
                     /locus_tag="PLM74_07345"
     CDS             complement(<1..877)
                     /locus_tag="PLM74_07345"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42924.1"
                     /translation="MSSCGSFALVLHSHIPYCRKSGVWPFGEEWVFEAMAETYIPILD
                     LLSAGGFEPRVALSLSPVLAEQLADSYMKSRFLEYAEMRKKAAVHDAERYEAEHDDRL
                     ALLAAEYAEYYEYVVDRFSNHYGGDLLGAIAGLASHGIIELLATAATHAYLPLLSEER
                     ARVQMRLGCEACERHFGVRPRGAWLPECGYRPGAIERVLTECGIDYFIVDTHAVAGGA
                     AREAYGAEAKCSGGALPGYCVSPSMATLRPYALSGSQMSVFARDEVTAAQVWSRETGY
                     PGDGSYREFHRRDSIS"
     gene            1007..1846
                     /locus_tag="PLM74_07350"
     CDS             1007..1846
                     /locus_tag="PLM74_07350"
                     /inference="COORDINATES: protein motif:HMM:NF027583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4912 domain-containing protein"
                     /protein_id="HON42925.1"
                     /translation="MHGLCGEVNAYQVPEGYGETRIVLQVRDPFWMHAYWEISRETAL
                     DLARELGESISSSRLTLRVNDMTMRDEADWGKQVAIDVHPFANNWYINVCEPDTSYCV
                     DLGLTHSADEFRFIARSNVVHTPRVGPSEVRDEEWLSIRALEAVARGPMVYTASPGMP
                     AQRIRAEEWAREIALGSGGVGAISSPVGPPVPTRAPEMAGRQYWLRADAELVVYGATE
                     PGSQVSVSGMPVNLASDGTFSVRIALPVGELPIEVAAESADRLMSRHISWTVARTGLK
                     DGR"
     gene            1853..3475
                     /locus_tag="PLM74_07355"
     CDS             1853..3475
                     /locus_tag="PLM74_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HON42926.1"
                     /translation="MSEPKGYLAIVLHAHLPYVRHARGEAYLEEKWYYEALTETYIPL
                     YTMLARLAAEGVPYRLTVSLSPTLMSMCRDELLQERFSEFLDGLCRLAELETSRTRWQ
                     PQFCETAKMYLERFQHVRSVYNETLNRDIIGGFARLAREGRIELITTAATHGYLPLME
                     VCPSAARAQIIAAANYYRSVFGTTPKGMWLPECGYYPGVDAYLREAGVRYFFTDAHGI
                     ENASPRPRYGVYAPIYCPSGVAAFGRDMESSKQVWSAHEGYPGDFDYREFYRDIAYDL
                     DWDYIRPFMGGCPARTHTGIKYYRVTGRTEHKQPYVREWAVNKAATHAGNFVFNRERQ
                     IEHLASVMDTKPIVVAPYDAELLGHWWFEGPEFLEFVIRKIAYDQNIIKLITPVDYLD
                     ERARAQVSVPSMSSWGYMGYSEVWLADCNHWIYPHLHMMARRMSELAHDYAMPQPDLI
                     RRALNQAARELMLAQSSDWAFIMKAGTCVDYAVGRTKSHISRFNRLYNDIRSSDVDHE
                     WVADLESIDNIFPDMDYMIYSASPMASAILDT"
     gene            complement(3433..5886)
                     /locus_tag="PLM74_07360"
     CDS             complement(3433..5886)
                     /locus_tag="PLM74_07360"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HON42927.1"
                     /translation="MKAVIMAGGKGTRLRPLTCTLPKPMVPVACRPMMEHVVSLAVAH
                     GFRDLACTLCYMPDTIQNHFGDGASFGCSIRYSIEETPLGTAGSVRALADFLDDTFLV
                     ISGDALTDIDLASAVEFHRHKGAAATIVLTRVETPLEYGVVIVDGDYRITRFLEKPSW
                     GEVFSDTVNTGIYVLEPDVMELVEASREFDFSKNLFPRLLADGYPMFGYVAEGYWCDV
                     GNLEQYRNTHLDILDGRVRVAIPGELVDEGVWIGEGAEIETGAVLRAPVVIGPRARVE
                     KGAVAGDYSVIGAASILAGGSSVRRSILWPGAFVGQNAQVNAAILASRVSVKAGATVL
                     EGAVVGSGSSIGERAQVKAGVKIWPDKVVDGGSQVNASLVWGAPWSKRLFGRLGVAGL
                     SNIEVTPDFAARLGAAYASCLPEGRIVTVSSDVHPASKMTTASLACGAMSIGATVADL
                     GEATTAVARYAVPALHAVGGMHARVSPADDNVTVIEFLDSRGINIDKALERKIENALL
                     TEDYRRAAASRVGGLLKETGMTALYLDAIERAIDADSIKRAGLRIVAGYDEDYLRDVM
                     NLISGALGCTLKKTRAFQGAWAMAELADAVVSEDAALGVLLDRNAERLALVNERGESV
                     CDEDVLSLICAALLKSRVGGTVVVPITAPGAIEKVANGLNGRVVRSQANPRSMMEKAI
                     EARVMMGEAGVPQFQPMLDGLFALAVLLEQLAQGSMPLSSMVSAMPRSFMTRRLVDCP
                     WQSKGRVMRHLIEETDNEEVQLVDGIKVYTDQGWTLILPDSEDPVFHVYSEAASQEIA
                     EEIAGIYVSRIAEAMGDAE"
     gene            complement(5883..>6092)
                     /locus_tag="PLM74_07365"
     CDS             complement(5883..>6092)
                     /locus_tag="PLM74_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42928.1"
                     /translation="WQFYAGSPNSLADAVLHVLHSPEAARKAAGVARREVLSTYDWGA
                     IARRTTDVYDEVLIDAAAQEEAMAR"
BASE COUNT         1253 a         1954 c         1700 g         1185 t
ORIGIN      
        1 ccgagatgct gtcacgcctg tggaactcgc ggtaactgcc gtcgcccgga taccccgttt
       61 ccctcgacca tacctgcgcc gcggtgacct catcccgggc aaacaccgac atttgcgagc
      121 cggagagcgc gtacggtctg agcgtggcca tgctagggct cacgcaatac ccgggaagcg
      181 caccccccga acatttagcc tcagccccgt atgcctccct cgccgctcct ccagcgaccg
      241 cgtgcgtgtc gactatgaag tagtctatgc cgcactcagt caacacccgt tcaatagccc
      301 ccggccgata gccgcactct ggaagccacg cgcctctggg cctgacgccg aagtggcgct
      361 cgcacgcttc gcagcccagt ctcatttgga cgcgcgctcg ctcctctgat agcagcggca
      421 ggtaggcgtg tgtcgcagcc gtagccagca gctcgattat gccgtgcgat gccaggccgg
      481 caatggcgcc cagtaggtcg ccgccgtagt ggttcgagaa cctgtccacc acgtactcgt
      541 agtactctgc gtattccgca gccagcaggg ccagcctgtc gtcatgctca gcctcgtatc
      601 tctcggcgtc atgcactgcg gccttctttc gcatttcggc gtattccaga aaccgtgatt
      661 tcatgtagga atctgccagc tgctcagcga gaaccgggga taaactaagg gcgacacgag
      721 gctcgaaccc acctgccgac aacaggtcaa ggattggtat gtatgtctcg gccatcgcct
      781 cgaacaccca ctcctcgccg aatggccaaa cccctgactt gcgacagtat gggatgtgtg
      841 aatgaagcac cagggcaaat gaaccgcaag acgacatatg ccaaacctcc ctcggcatga
      901 agtattctta cccaaccgcc gtggcctatc ccggcgggat aaccccggtt cacgccggat
      961 agaataccag agagattgcg tttcggtgtc gtgaggaggt cctttagtgc acggcttgtg
     1021 cggcgaggtg aatgcgtacc aggtacccga gggctacggc gaaacccgga ttgtgctgca
     1081 ggttcgcgat ccgttctgga tgcacgccta ctgggaaata tccagggaga cagcgcttga
     1141 cctcgcccgc gagctcggag agagcatctc gagcagtcgc ctcactctca gggtaaatga
     1201 catgaccatg cgcgacgaag ccgattgggg caagcaagtc gcgatcgacg tccatccctt
     1261 tgcgaacaac tggtacataa acgtatgcga acctgacacg tcatactgcg tggacctggg
     1321 tctgacacac agcgccgatg agttccggtt catcgcacgt tccaacgtgg tgcacacgcc
     1381 gcgggtggga ccctcagagg tccgcgatga ggaatggctt tccattcgtg cactggaagc
     1441 agttgcccgg ggtcccatgg tctacactgc gtcgcccggc atgccggccc aaagaatccg
     1501 tgccgaggag tgggcgcggg aaatcgctct gggctcggga ggagtaggcg cgatttccag
     1561 cccggttggc ccgcccgtgc cgactcgcgc cccggaaatg gccgggcgcc agtactggct
     1621 gcgggcagat gcagagctcg tagtatacgg ggctacagag ccgggatctc aggtaagcgt
     1681 gtctggcatg ccggtgaact tggcttctga tgggaccttc tcggtgagga tcgccctccc
     1741 tgtgggagaa ctccccatcg aggtggctgc cgagtcggcc gatcggctga tgtcacgcca
     1801 tatatcgtgg accgtcgctc gaaccggact caaggacggc aggtgataga cgatgagtga
     1861 acccaaaggt tacctggcaa tagtgctaca tgctcatctt ccttacgttc gccatgcgcg
     1921 cggcgaggcg tatttggagg agaagtggta ctacgaagct ctgacggaaa cgtatatccc
     1981 gctgtacacg atgctggctc ggctggccgc agagggagtg ccgtaccgcc tcacagtatc
     2041 gctctcccct actctgatgt cgatgtgccg ggacgagctt cttcaggagc gcttttccga
     2101 gtttctagac ggcctgtgtc gcctggccga gctcgagaca tctcggacca ggtggcaacc
     2161 gcagttctgt gagaccgcca agatgtacct ggaacggttc caacacgtca gatccgtata
     2221 caatgagact ctcaatcgcg atatcatcgg agggttcgcg cgccttgccc gcgagggccg
     2281 aatagagctg ataacgactg cagcgaccca cggctatctg ccgcttatgg aagtgtgtcc
     2341 gtctgcggcc cgtgcgcaga tcatcgcggc cgccaactac taccgcagcg tattcggaac
     2401 cacgcccaaa ggcatgtggt tgccggaatg cggttactac ccaggcgtcg acgcatactt
     2461 acgagaggcc ggagtccggt atttcttcac tgatgcgcac ggaatagaaa acgccagccc
     2521 caggccgaga tatggcgtgt acgcgcccat ctactgccct tcgggcgtgg cggcgttcgg
     2581 gcgagacatg gaatcttcca agcaggtctg gagcgcacat gagggatacc ccggcgactt
     2641 cgactacagg gagttctacc gtgacatcgc ctatgatctc gactgggact atatacggcc
     2701 gttcatgggc ggttgcccgg cgagaacgca cacaggcatt aagtactacc gcgtcaccgg
     2761 gcgcactgaa cacaagcagc cgtatgttcg ggaatgggcc gtgaacaaag ccgccacaca
     2821 cgcgggcaac ttcgtgttca accgcgagcg gcagatcgaa cacctggcat cagtcatgga
     2881 cacaaaaccg atagtagtcg ctccttacga cgctgagctc ttagggcact ggtggtttga
     2941 aggccccgag ttcctggagt tcgtcatacg caagatcgcc tacgaccaga acatcatcaa
     3001 gctcattacg cccgtagact acctggatga gcgcgcacgc gctcaggtgt ccgtaccgtc
     3061 catgtcatca tggggctaca tgggctatag cgaggtgtgg cttgcagact gcaatcactg
     3121 gatatacccg catcttcaca tgatggcgcg tcgcatgagc gagctcgccc atgactacgc
     3181 tatgccacag cccgatctga tccgacgagc tctcaaccag gccgcccgcg aactcatgct
     3241 tgcgcaaagc tccgactggg cgttcatcat gaaggcaggc acttgcgtgg attacgctgt
     3301 agggcgtacg aagagccaca tatccaggtt caaccgtctc tacaacgata ttcgcagctc
     3361 ggatgtggat catgagtggg tggctgacct ggaatcgatc gacaacatat tccctgacat
     3421 ggactatatg atctactcgg cgtcgcccat ggcctccgcg atcctggaca cgtaaatgcc
     3481 ggcgatctcc tccgcgatct cctgacttgc cgcctcgctg taaacgtgga agaccggatc
     3541 ttcagagtcg ggcaagatga gcgtccagcc ctgatcggta tagaccttaa tgccatccac
     3601 tagctggact tcttcgttgt cggtctcctc gatcagatgc cgcatcaccc tgcccttcga
     3661 ttgccacggg caatccacaa ggcgccgcgt catgaaggat cgtggcattg cgctcaccat
     3721 ggacgaaagc ggcatgctgc cctgcgcaag ctgttccagc aacacggcta aggcgaaaag
     3781 gccgtccagc atgggctgaa actgcggaac gccggcttcg cccatcatca ccctggcctc
     3841 gatagccttc tccatcatgg acctggggtt ggcctgcgac cgcaccacgc gcccattaag
     3901 gccattcgcc accttttcga tggcaccggg agcagtgata ggcaccacta ccgttccgcc
     3961 cacgcgcgac ttgagcagtg cagcgcagat cagggacaac acgtcctcat cacacacact
     4021 ttcgccgcgt tcattcacta gcgccaaccg ctctgcgtta cgatcgagca ggactcctag
     4081 cgcggcgtct tctgacacga ctgcgtccgc gagttcagcc atggcccacg cgccctggaa
     4141 ggcacgtgtc ttctttagtg tgcagccaag cgcaccggaa atcaggttca tgacatccct
     4201 caggtagtct tcatcgtatc ctgccacaat gcgaagacct gccctcttga tcgagtccgc
     4261 atcaatggcc cgttctatgg catccagata cagcgccgtc atccctgtct ccttaagtag
     4321 accgccgaca cggctcgcag cggctctgcg gtaatcctcg gtgagcaagg cattctctat
     4381 cttgcgctcc agcgccttat cgatattgat gccccgcgag tcaagaaact cgatgaccgt
     4441 aacattatca tcggccggcg atactcgtgc atgcatgcct ccgaccgcat gtagcgcagg
     4501 cactgcgtat cgtgccaccg cagtagtcgc ttcgcccaga tcggccacgg tcgcgccgat
     4561 tgacatggcg ccgcaggcca aagatgccgt cgtcatctta gaagcagggt ggacatcgct
     4621 cgacactgtg acaattcgcc cctcgggcag gcacgaagca taggccgcgc ccaggcgggc
     4681 ggcaaagtcc ggagtcactt ctatgttgga tagcccggct acgcccaacc gtccgaagag
     4741 gcgctttgac catggcgctc cccacacgag gctggcattg acttgcgatc ccccgtccac
     4801 caccttgtcg ggccatatct tcacacctgc cttcacctga gctcgctcac ctatggagct
     4861 tcccgagccg acaactgccc cctcgagcac ggttgcaccg gccttcacgc taaccctgga
     4921 cgcaagaatg gccgcattta cctgggcgtt ctgcccgacg aacgcgcccg gccacaatat
     4981 agagcggcgc acggaggagc cgccggccaa tatggacgcg gctcctatca ccgaatagtc
     5041 gccggccacg gcgcccttct ccactctcgc ccgaggcccg atcaccacag gcgccctgag
     5101 aaccgcaccc gtttcgatct cggctccctc accgatccac acgccctcat ccaccagttc
     5161 tccagggata gccacgcgga ctcgtccatc cagaatatcc agatgagtgt tgcggtactg
     5221 ctccaggttg cccacatcac accagtatcc ttcagccacg taaccaaaca tcggataccc
     5281 atcggccagg agccgcggga acaggttctt gctgaagtcg aactctcgcg aagcctcgac
     5341 cagctccatc acatccggct ccagcacata gatccccgtg ttcacggtgt cgctgaatac
     5401 ctctccccag ctgggcttct ccaaaaagcg cgtgatacgg tagtcgccgt ctactatgac
     5461 cactccgtac tccagcggag tctccactcg cgtgagtaca atggtcgcag ccgcgccctt
     5521 atgcctgtgg aattctacgg ctgaagcaag atcgatatcg gtcagggcgt cgccgctaat
     5581 caccaggaac gtgtcatcta ggaagtcagc cagcgcgcgc acgctgcccg cagtgccaag
     5641 aggtgtctcc tctatggaat acctgataga acacccgaag gaggcgccgt cgccgaaatg
     5701 attctggatg gtatcaggca tgtagcagag ggtgcacgcc aagtcgcgga agccgtgcgc
     5761 cacagccagg ctcacaacat gctccatcat gggtcgacac gccaccggca ccatgggctt
     5821 ggggagcgta cacgtgagcg ggcggagccg agtgcccttg cctcctgcca tgatcaccgc
     5881 tttcacctgg ccatcgcctc ctcctgcgct gcagcgtcga ttaggacctc gtcatacaca
     5941 tcggtggttc tgcgagcgat ggccccccaa tcgtatgtcg acagcacttc gcggcgggca
     6001 acccccgcgg cttttctggc tgcttccggg ctatgcagta cgtgcaagac ggcatcagcc
     6061 agcgaattag ggctgcccgc atagaactgc ca
//
LOCUS       DAOTAC010000151         6009 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_11111_length_6009_cov_4.073900, whole genome shotgun sequence.
ACCESSION   DAOTAC010000151 DAOTAC010000000
VERSION     DAOTAC010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6009
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11111_length_6009_cov_4.073900"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="PLM74_07370"
     CDS             complement(<1..527)
                     /locus_tag="PLM74_07370"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HON42929.1"
                     /translation="MSGIDRKDLKGVFAPIPTPLGEDGEVSLDAVRGNMERWASSGLA
                     GVVVFGSNGEAPYLSWDEKVHLIEAVIQANAGRKLVIAGTGQETTRDTIRLTRKAAEL
                     GAQAALVLPPHFYKNAMTNAALAAHYRAVADESPIPVIIYNMPGNTGLNLGSELVAEL
                     SNHPNIAGLKDSSGN"
     gene            complement(669..1505)
                     /locus_tag="PLM74_07375"
     CDS             complement(669..1505)
                     /locus_tag="PLM74_07375"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HON42930.1"
                     /translation="MNGQSADPATSGVRAASGIAVTGVATSGIARFAKLAREYWWLLH
                     GQLHQLRLQWFWYVVMMSFVPLVTMAFLWFFTGRSPEALLFIITGSITTALCTSAMLT
                     LGQNIGYMRDAGSFEYYASFPISKPVFIFALATRGVILSIPSSAILLAIGVFVFRVPM
                     RPSPVTIIIFILAGYSLAGLGAFVGFLSRDGQTAGLVTQIIDPLIIFLAPVYIPGEHL
                     PRFLQYTSRFIPTTYVANALRASVAVSVTSQTWLEIGLLVVWTVLTLWAASRKLAWRA
                     NE"
     gene            complement(1486..2436)
                     /locus_tag="PLM74_07380"
     CDS             complement(1486..2436)
                     /locus_tag="PLM74_07380"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON42931.1"
                     /translation="MDHACVVDGLCKTYGATGVVANRDLNLRVSPGEVLGLFGPNGAG
                     KTTLVRQIMGLLRPTSGSISISGHDVVRNPGAAPVYAAYHAQRQVMLASYRATEVLVQ
                     AGVLRGMRTRDAVSQARALLERFGLAARSGSLMLRLSGGEGQMVLLLSTFMGYRPLVI
                     LDEPTSNLDPVRRRLVWDFLSEKAREGVAVMLVTHNVAEADHAVHRAVVINRGTVIAD
                     GTPGDLKRMVSRKIEIRVSLKPGAEWLLADTARGEPLGEGRWMIRVDPEESSQVLSHI
                     IDTQGLDGIDDFTVSGVSMDAVYEALTGEWWDERHERTVS"
     gene            complement(2494..2673)
                     /locus_tag="PLM74_07385"
     CDS             complement(2494..2673)
                     /locus_tag="PLM74_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42932.1"
                     /translation="MIITIANVKVEVSRAPRRRPTMNWDGIGRAYEGQLVERAARAAR
                     LAHEVKASTYLPLLG"
     gene            complement(3268..4056)
                     /locus_tag="PLM74_07390"
     CDS             complement(3268..4056)
                     /locus_tag="PLM74_07390"
                     /inference="COORDINATES: protein motif:HMM:NF033068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 3'-phosphotransferase"
                     /protein_id="HON42933.1"
                     /translation="MTELSMPADIAEIIGNGILSRNTVGCSASAVYRVQGLAGGGNAY
                     LKILKAVEDDDLRREHDVLKWLNSRLPVPRVLGFAQEGLTQYLLMTETPGSSAEDEVQ
                     TTESEQVISRLAEALHVVHGVNISACPFDMRLDVKLAEARARIDSGLVDEGDFEGANK
                     GRSAMDIYRELVATRPNDEELVFTHGDYCLPNIMLKDGAVSAFLDLGRAGIADRYTDL
                     ALAARSIRHNTGDELLVDLFFEAYGLGEVDWRKVDYYILLDELF"
BASE COUNT         1245 a         1848 c         1655 g         1261 t
ORIGIN      
        1 atattgccag agctatcctt cagccctgcg atgttgggat ggttcgacag ctcggccacg
       61 agttccgagc ccaggttgag gcctgtgtta cccggcatgt tgtagatgat caccggaatc
      121 ggagactcat cggcgaccgc tctgtagtgg gcggccaaag cggcatttgt catggcattc
      181 ttgtagaagt gcggcggcaa taccagcgct gcctgcgcac cgagctctgc tgccttccgc
      241 gtcagccgaa tggtgtcgcg tgtggtctcc tgccccgtgc ccgcgatgac cagctttcgt
      301 cctgcattcg cctggataac ggcctctatc aggtgcacct tctcgtccca cgacagataa
      361 ggcgcttcac cattcgaccc gaacactaca accccggcaa gcccagaact agcccaccgc
      421 tccatgttgc cgcgaaccgc gtctagtgag acctcgccgt cctcgcccag cggcgtcgga
      481 atcggagcga acacaccctt aaggtccttc ctgtcgatac cagacatgag catgcctcgc
      541 tttctcaatc gatgcagccc aattgtatcg taccttgact gagttcgcta aagccagacg
      601 cttggcgcca agtagtttcg ggctccacgc tctcgatcat gcggccccca ctgaacgaac
      661 cgtggacgtc actcattggc ccgccaagcg agcttacgag atgccgccca cagcgtaaga
      721 acggtccata cgaccaggag accaatctcg agccaggttt gggatgtgac gctaacggcc
      781 actgatgctc gtagggcgtt cgcgacatag gtagtcggga tgaacctcga cgtatactgc
      841 agaaaccgtg gcagatgctc tccgggaatg tacaccggcg cgaggaagat aatgagtgga
      901 tcgattatct gagtaacaag cccagcggtc tgcccgtctc ggctcaagaa gcccacaaac
      961 gcacccaggc cggcaaggga gtagcctgcc agaatgaaga tgataatggt gactggagac
     1021 gggcgcatag gcaccctgaa gacgaaaacg ccgatcgcaa gaagtatcgc agatgaagga
     1081 atcgaaagta tcacaccccg agttgccagg gcgaatatga acacgggctt cgaaatcggg
     1141 aagctggcat agtactcgaa gcttcccgcg tctctcatat agccaatgtt ctggcccaga
     1201 gtcagcatag ccgatgtgca cagggcggta gttatgcttc ctgtgattat gaagagcaag
     1261 gcctccggcg acctaccggt gaaaaaccac agaaaggcca ttgtgaccag ggggacaaac
     1321 gacatcatga ctacgtacca aaaccactgc aatcgtagtt ggtgaagctg gccgtgcaac
     1381 agccaccagt actcgcgggc caatttcgcg aaacgtgcta tgcccgatgt tgcaacgccc
     1441 gttactgcga tgcccgacgc tgcacgaacg cctgaggtcg cggggtcagc tgactgtccg
     1501 ttcatgtcgc tcatcccacc attctccggt caatgcctca tacaccgcat ccatgctcac
     1561 acctgagact gtgaagtcgt cgatgccgtc cagaccttgt gtgtcgatga tgtgtgaaag
     1621 aacttgcgag gattcctccg gatcgacccg gatcatccat ctgccctcgc caagcggctc
     1681 gccccgagcg gtgtcggcca aaagccattc tgcgcccggc ttgagcgaca cgcgaatctc
     1741 gattttgcgg ctcaccatcc gcttgagatc acccggagtg ccgtctgcaa tcaccgtccc
     1801 ccggtttatc acgaccgccc ggtgcacggc atggtcagct tcggccacgt tgtgagtcac
     1861 cagcatcacc gccacacctt cgcgcgcctt ctcgctcagg aagtcccaca caagccgacg
     1921 ccgcactgga tcgaggttgc tcgtgggctc gtccagaatg accagcggac gatatcccat
     1981 gaaggtggaa agcagaagga ccatctgccc ctctccgccg ctcagacgga gcatcaagct
     2041 gcctgaccgt gccgcaagcc caaaccgttc taggagagcg cgcgcctgac tcaccgcatc
     2101 ccgagtacgc atcccccgca acacacccgc ttgcaccagc acttctgtcg cgcggtagct
     2161 ggccagcata acctggcgct gcgcgtggta tgcagcatag accggcgcag cgccagggtt
     2221 acgcacaaca tcgtgtcccg agatgctgat cgaaccactc gtggggcgaa gcaggcccat
     2281 gatctgcctg actaatgtgg tcttgccggc gccgttaggg ccaaacagcc caaggacctc
     2341 ccccggggaa acccgcaggt tcagatctcg attggcgaca acacctgtgg cgccatacgt
     2401 cttgcagagg ccatccacaa cacaggcgtg atccatcgtt ttccgccacc cccttctact
     2461 gcgccgaggc tcgcggcgtc cacgttcgag cgcttacccc aacagcggaa gatacgtgct
     2521 cgccttaacc tcgtgcgcca gccgagcagc tcttgcagcc ctctcgacca gctgcccctc
     2581 gtatgcccga ccgatcccgt cccagttcat cgttgggcgg cgacggggcg cccggcttac
     2641 ctctaccttc acgtttgcta tcgtgatgat catgatcaac acctccgttt gggtcgaacc
     2701 tcaatattat tgatatcgtc atcaatatat taaattgcgt gctgcagaat gtcaagtgct
     2761 ccttggttcg atttggcgtc aaatcgtaca tgtgggtgac tgcgttaacc cggcatgcgg
     2821 tagccgcggg acgcaaatgg cggcttagct ctacacgtta ccggcattcc tgaagaggcc
     2881 agggcatgaa ggttgaatcg tcgcgaattc atcgctgttg ggacgggggc ctagcggtct
     2941 ggaagagagg gggcgcatgt agggatcata ggtaacgacg gcgagcccat tatcggcttt
     3001 ggatcatgtg tctccaaggc ccgagttaca gcctgtcgtg cagccgacag gtctggcgga
     3061 aaacctgtcg tctacccgac aagttgcttt cagtccgcgt ctgtatgctc tcgtctgccc
     3121 gcggcatctt gtcgcattag cgttttcatc ttgacacagg ggccggacgg caatcctgca
     3181 gtcaggtcgg gatccactag cgacatgacg tcgtgaagcc tcgcttcatc caattcctag
     3241 cccgtagtaa gcggagatag tgaaggctca gaacagctcg tccaggagta tgtagtagtc
     3301 gaccttccgc cagtccacct cgcccaggcc gtatgcctcg aagaagaggt cgaccaacag
     3361 ctcgtcgcct gtgttgtgcc ttatgcttct ggccgctaga gccagatcag tgtacctgtc
     3421 agctatgccg gcgcgtccaa gatcgagaaa tgcgcttact gcgccgtctt tcagcatgat
     3481 gttcggcaga cagtagtcgc cgtgggtgaa aaccaattcc tcatcgttgg gcctggtcgc
     3541 gacaagttcg cggtagatgt ccatcgcgct tctgcccttg ttcgcgcctt caaaatcccc
     3601 ctcgtccact agccccgagt ctatcctggc tcttgcttcg gcaagcttca catcgagtcg
     3661 catgtcgaat gggcaagccg aaatgtttac accatgaacc acatgcagtg cctcggccag
     3721 tcggctgatc acctgttccg actctgttgt ctgcacctcg tcttcggcgc tagagcccgg
     3781 cgtctcagtc atgagcaagt attgggtcag cccttcctgc gcgaagccca gcactcgggg
     3841 cacaggaagt cgcgaattca gccacttgag cacatcgtgt tcgcgacgca ggtcatcgtc
     3901 ttccaccgcc ttgaggatct tcagataggc gttcccgcct cccgccaagc cctgaacgcg
     3961 gtaaaccgcc gaggctgagc accccacagt attgcgtgac agtatgccgt ttccgatgat
     4021 ctccgcgata tccgcgggca tcgatagctc agtcaagatg gatcactccc gatttctact
     4081 ccagtatgga acggtgatca aggcccgcac cgatccaaac aggccggctg tgaatgccac
     4141 aggcgacaca agggttcacc aatgagcatg gccagattcg cgatctccca cttgatctcc
     4201 tctcttggcg agtagggagt gcgtgcaggt tttgtgggca atagcaattg gaaaagcggg
     4261 tagacatcgc cacgcgggat ggtgagccgg aaaatgacct cctccccgat cattgcggcg
     4321 ggtcattggc tgagggggtc atgctcacgt cgctggaatg gctgtttagc tggtcttggc
     4381 atcatggctg tcggcaaaac ggctggagct gggatatcgt cgctttttgc catcagtttc
     4441 ggagataccc gcaacttttg agtacatcga tttcgaaggg ccacctgact gcctccgaat
     4501 cacgaaaatc ggggggtatc cgacggattt gggtagtgaa gcaagcgggc gggccgacat
     4561 aatatcccac cgaatggaac tattcgggaa aggcgcgcaa ggcgtggcgc ggcgtgccag
     4621 ctattcgcca ctaaataggc gatctcttgc ctcgagggct cctcttgcgg ggtcgactcg
     4681 ccccccgcgg accccggccc aaatcgatgt actcaggaat tgcgtttttc gccgaaagta
     4741 atgttaaaat gtgaccgtat ctctagctgg atacccccgg gggtatatcc ctgagccaca
     4801 gcagtcatct ttccctgcct cagcacgccc ccggatgtac tcctggattc gttcctggcc
     4861 tattcctgga cctacccgcg cgtctatcgc cggacccctg gtctcggtgc gattccaact
     4921 gcttccgctg tggccccggc gagaccaccc gcctgaatgc aggggatctg cgaccccgga
     4981 agccacaatc cctacacgca cccgcgagta ggtgcgtact taacatgaac agccttgtgt
     5041 tgggcgaaag ggttcgcaca tgagccgggt cggccctatg atgccgcgac caagtcctgg
     5101 ggcccaaccg atgttgcaga acgcccaagt cgggcgcaag ccctctggac gcagtgcgat
     5161 aacaggttgt ggtgatgctg atgatagcgt cctcactcag agccgtgatg atcactaccg
     5221 tgcttgcatt gatcgaaccg gcgggcatat cgcacggtct cttccgcacc ctgccgaaat
     5281 atcctacggt tgaacgcgat ctgggaagac tgaaggatgt tctggccgtt ctagctgggc
     5341 ctgcagacat ttgcagtctc ccaggccgga aacggagatt tttcgccgtg tgagtgcaga
     5401 aaatctccgc cgtgctggtt tcggaccgct atgctgccac agaacgccag tcgccgcgtc
     5461 atgttagcgc cagtaatagc cgtatctacc catctgcatg cacctgtccc atatatgagc
     5521 gccccaaggc gcctcgcctg ctcctgcatg ccgcttattg gcgggctttt agtataataa
     5581 gtaatctagg cggacccacc cggaagtttt tcgccgtgtg agtgcagaaa atctccgtcc
     5641 aacggctcca aaggggagaa aagctgcccc cgataccccc tgtaggtatc caccatcgcg
     5701 ccgcgccgaa atcgatgggt gagggcttgc tggctggtcc ctcctgaagt acgagctccc
     5761 cctcttccac ccgactctcc ctgctggttt tctccttcag gcagaccggg gttgaggggg
     5821 aggcatcgcc tttctgaccg ctcggtaagc tcgtaccagg ctcaattgga ggggctgttc
     5881 agaaggtcca ttactatgct gcatatgtcg gtcaccgaat gcgccgccct gaggagctgc
     5941 tgagcctttt cgctccgcgc tcacggaccg tatacgatca agggcacgaa cacatccgag
     6001 tccaggtcg
//
LOCUS       DAOTAC010000152         5869 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_11435_length_5869_cov_4.291022, whole genome shotgun sequence.
ACCESSION   DAOTAC010000152 DAOTAC010000000
VERSION     DAOTAC010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5869
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11435_length_5869_cov_4.291022"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /gene="hisS"
                     /locus_tag="PLM74_07395"
     CDS             complement(<1..902)
                     /gene="hisS"
                     /locus_tag="PLM74_07395"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018703145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HON42934.1"
                     /translation="MGIVAPRGTFDIMPDDAGLWQFVEGLVRETFERCGYREIRTPMF
                     EHTELFERGVGSTTDIVEKEMYTFKDRGGRLITLRPEGTAPAVRAFVEHGLAGRALPA
                     KLYYMGPMFRYERPQAGRYRQFHQFGMEVMGVAGPEADAEVIAAPLEIFRAMGIQDVV
                     VNLNSIGCPVCKPAYRERLREAYAPVLDRLCPSCKSRYDRNPLRLLDCKSEECRDALP
                     EPPTIFGQLCPECSAHFDGLRRLLDKLGVGYVINPRLVRGLDYYTKTTFEYNVGGIGS
                     QDAIGGGGRYDGLVEECGGPPTPGV"
     gene            complement(998..2179)
                     /locus_tag="PLM74_07400"
     CDS             complement(998..2179)
                     /locus_tag="PLM74_07400"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HON42935.1"
                     /translation="MISGMRKRMGVFVAVIAGLLALGMIIPMLFTGTSPGGAAARLTV
                     AKVNRERVSALDLSREFYRLYYYRTSFGGLKAEDLDALRSQALDSLVEQAIILDAAKS
                     AGLKPNQEQVEASYKAEVGYFKSEQEFAQAMAGWAYTPKTYKQELVKRQLIDKYPLYA
                     KPVEVTEDDVNAEFERAQKTNPQLTLDEARESIEKLVRARKEKEARDSLLAELREKAK
                     IKIVDSQVLAYRAMQDGKFDEAVKLYRKAIKQSPEDPYLQVSLGRALAAAGKGDDSKA
                     AFEKAVKLRAGDRFILIAQADSLKEAGETDAAAEVYRHAAEVAGEDIFAHGLILEAFE
                     EMGLKEDAQAQKDKIDALQKALIERYEAQQKAAETAEPETAEPEAAEPDDVEDAGASQ
                     E"
     gene            complement(2243..2899)
                     /locus_tag="PLM74_07405"
     CDS             complement(2243..2899)
                     /locus_tag="PLM74_07405"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HON42936.1"
                     /translation="MAEKGTLRVDRIPVGPLSTNCYVVWREGSSVATVIDPAAYAERI
                     AGRLEQNSLTCGAIINTHGHPDHIGANGALRKLTGARVYVGAEDSHMLACPDDMFRVL
                     ARSGGEGDKLDADEILAGGDRLSVGEVEFYVLATPGHTPGGISLWLPDDLAVFSGDTL
                     FKMGVGRTDFAGGDEAALFDSIYRVLFSLDEFTKVYPGHGPDTTIAAEMAWHGRSRSR
                     "
     gene            complement(2939..3388)
                     /gene="dtd"
                     /locus_tag="PLM74_07410"
     CDS             complement(2939..3388)
                     /gene="dtd"
                     /locus_tag="PLM74_07410"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004102462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HON42937.1"
                     /translation="MRAVVQRVKSAQVSVDGKLVGRIGPGLMVLLGVAEGDGPEDVRY
                     MVDKIPNLRVFDDADGKMNLSCLDVGGSVLAVSQFTLLGDCRRGRRPSFTAAAGPDLA
                     NALYEQFVQGVRELGIVVETGVFRAEMEVSLTNHGPVTIILDSSKSF"
     gene            complement(4003..>5869)
                     /locus_tag="PLM74_07415"
     CDS             complement(4003..>5869)
                     /locus_tag="PLM74_07415"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HON42938.1"
                     /translation="LSRMDVKTREEQQAESLRKMFVAMAKDIRVILIKLADRLHNMRT
                     LRECQPDKQKRVAGETCEIYAPLAHRLGMWRIKWELEDLALRYLEPGVYYELVEKVAT
                     KRQEREGHIEEARQTLSAALEEIGVPAEIQGRPKHFYSIYQKMIKQGKDFSEIWDLMG
                     LRVIVGTLRECYAVLGAVHSIWKPIPGRFKDYVAMPKSNMYQSLHTTVIGPRGEPLEI
                     QIRTWEMHRTAEYGIAAHWRYKDGGVGGGDFEDKITWLRQILDWQKETHNGTEFVETL
                     KVDLFSDEVYVFTPKGDVKTLPAGSTPIDFAYAVHTDIGNRCAGARVNGRMVPLGTPL
                     ATGDIVEIVTNKAQTGPSRDWLNIAKTSKAKGKIRQWFKEQRREESIERGKEMLERDL
                     RRMGHEVHANLKEERLAEAARKFSYQSADDLLASIGYGKLSAAMVVGKLVPEKAAEKS
                     PESVFEEVARPLPGAVRASRSTHGVVVRGIDNVLVRFARCCNPVPGDPIVGYITRGRG
                     VSVHREDCPNISSMMGEDEGRQVDVSWDMGNATSYPVEIEMEASDSPGLLTGVMNAVL
                     EMKAHVSAVNARTLRNRMALINMTVEINDVAHMNNVIGRLKRLQGVQNVHRARPS"
BASE COUNT         1092 a         1980 c         1525 g         1272 t
ORIGIN      
        1 acaccaggcg tgggcggccc accgcattcc tccacgagcc catcgtagcg cccacctccg
       61 cctatggcat cctgtgagcc gataccgccc acgttgtact cgaacgtggt cttggtgtag
      121 tagtccagcc cgcgcactaa acgcgggttg atcacgtagc cgacgcccag cttatcgagg
      181 agccgcctta gtccatcaaa gtgggcgctg cactccgggc agagctgacc gaatatggta
      241 ggcggctcag gcagagcatc ccgacattcc tcggatttgc agtcgagcag ccgaaggggg
      301 tttcggtcgt aacgcgactt acacgacggg cacagcctgt ccagcacagg cgcgtaggcc
      361 tctcgcagtc gctcgcgata ggcaggcttg cacacagggc acccaatgga gttgaggttg
      421 accacaacat cctgaatgcc catggcacgg aagatctcca gcggtgcggc aatgacctcg
      481 gcgtcggcct ccggaccggc aacgcccata acctccattc cgaactggtg gaactggcga
      541 taccgccctg cctggggtcg ctcataccgg aacatggggc ccatatagta tagcttggcc
      601 ggcagtgccc ggccggccag cccatgctcg acgaaggcgc gcacagcggg ggccgtcccc
      661 tcgggcctca gtgtgatgag ccgcccacct ctgtctttga aggtgtacat ttccttctcg
      721 actatgtcgg tagtagaccc cacgccccgc tcgaacaact ccgtatgctc aaacatgggc
      781 gtacgtatct ccctgtatcc gcacctctcg aaagtctccc tcaccagtcc ttcgacgaac
      841 tgccacagcc cagcatcgtc aggcattatg tcgaaagtgc ccctcggtgc aactatcccc
      901 acggcgaaca ccctcccgca atcctcatcg cccaacccgg gccgtcagct atggaaacgg
      961 gcgggcccag gcccgcccgc tcacgcacct tcgccggcta ctcctgcgag gccccggcgt
     1021 cctccacatc atcgggctca gcggcctcgg gctcagcagt ctcaggctca gcagtctctg
     1081 cagccttctg ctgggcctcg taccgctcga tcagcgcctt ctgcaacgcg tcgatcttat
     1141 ccttctgggc ctgtgcgtct tccttcaagc ccatctcctc aaaagcctcc aggatcagtc
     1201 cgtgcgcgaa tatgtcctcg cctgcaacct cggccgcgtg ccggtacacc tcagccgccg
     1261 cgtcggtttc accggcctcc ttgaggctat cggcctgggc tatcaggatg aaccggtcgc
     1321 ccgctcggag ctttaccgcc ttttcgaaag cggccttaga atcatcgccc ttgcctgctg
     1381 ctgccagcgc tctgcctagt gaaacctgga gatacggatc ctcaggcgac tgcttgatgg
     1441 cctttctgta cagcttcact gcttcatcga atttcccatc ctgcatcgcc cggtatgcca
     1501 gcacctgcga gtcgactatc ttgatcttgg ccttctcgcg cagctcggcg agcaagctgt
     1561 cgcgcgcttc cttttccttg cgcgcacgca caagcttctc aatgctctcg cgagcctcgt
     1621 ccagagtgag ttggggatta gtcttctggg cgcgctcgaa ctcggcgttt acatcgtctt
     1681 cagtgacctc gaccggcttc gcatagagcg gatacttgtc gataagctgg cgctttacga
     1741 gctcctgctt gtaggtcttt ggagtatagg cccaaccggc catggcctgg gcgaactcct
     1801 gctcgctctt gaagtagccc acttccgcct tgtaggatgc ctccacctgt tcctggttgg
     1861 gctttaggcc tgccgacttg gctgcatcaa gtatgatagc ctgctccacc aggctatcca
     1921 atgcctgcga acggagagcg tccaggtcct ccgccttcag ccctccgaat gaagtgcggt
     1981 agtagtacaa cctgtagaac tcgcggctca ggtcgagggc ggatacgcgc tcacggttga
     2041 ccttggccac tgtaaggcgg gctgccgcgc cgccgggact cgtgccggta aagagcatag
     2101 ggataatcat tccgagggcc aaaagacccg caatcacggc cacaaacacg cccatacgtt
     2161 tacgcatacc tgatatcaag gcaataccct cccagctgac ctgtgtaacc cacgtcattg
     2221 taacctacag aattgcccag tgtcaacgcg aacgcgatct cccgtgccat gccatttccg
     2281 ctgctatagt ggtatcgggc ccgtgacccg ggtacacttt cgtgaattca tccaggctga
     2341 agagcactcg ataaatagaa tcgaaaagcg cggcctcgtc gcctcctgcg aagtcggtgc
     2401 gtcccacgcc catcttgaac agggtatcgc ctgaaaacac agccaaatcg tcgggaagcc
     2461 acaggctgat gccgccagga gtatgacccg gcgttgccag cacgtaaaac tccacctcgc
     2521 ccacactcag ccgatcgccg ccggccagaa tctcatctgc gtccagcttg tcgccttcgc
     2581 cgcctgacct ggccagtaca cggaacatgt cgtcgggaca tgccagcata tgcgaatcct
     2641 ccgccccaac gtacacgcgg gcgcccgtca gctttcgcag cgcgccgttg gccccaatat
     2701 ggtcaggatg accgtgggta tttataatcg ccccgcatgt caggctgttc tgctccaacc
     2761 tcccggctat ccgctccgca tatgccgccg ggtcaatcac cgtcgcaaca ctcgatcctt
     2821 cccgccatac cacatagcag ttcgtcgata gcgggcccac aggaatcctg tccactctga
     2881 gagtgccctt ttccgccact agccggcctc ctcctcgccc gatcgttctg gttccggctc
     2941 agaacgactt tgaactatcc agaatgattg tgactgggcc gtggtttgtc aggctgactt
     3001 ccatttccgc cctgaatacg ccggtctcca caacgatacc aagctctctg acgccctgaa
     3061 cgaactgctc gtagagggcg ttggccaagt caggcccggc cgcggccgtg aaactagggc
     3121 gacgcccacg tcggcagtcg ccgagcagcg tgaactgcga aaccgccaga acgctgccgc
     3181 ccacgtctag gcacgagagg ttcatcttcc catcggcatc atcgaaaacc ctcaggttgg
     3241 gtatcttgtc taccatgtag cgtacatcct caggcccgtc gccctcagcc acgcccaaca
     3301 gcaccatcag ccccgggcct atgcgaccca ccaacttgcc gtctacactc acctgagcac
     3361 tcttcactcg ttgcactacg gctctcagct gaatccacca cccgtcctaa cccctactaa
     3421 ctctacgcgt tacccatgcc catgggtgtt tctaggtgcg gcgtccgtct gtaatgggcc
     3481 ctaacggggg ttctggcatc ctgctcgccc tggaatggga ttccgtgatc tccgatatgt
     3541 gcccatttgc cgcctaacca gattctggat catcctcctc gttttcgagc aggtttttcg
     3601 cctatcctac tcagatcctg ggtggtctgg caggtacgag ttgtcagaat tcgcgctggc
     3661 aacggcgctc cctccttatg gagtcacgcc cgtggcaaat ccgcaggtag aggcggatgg
     3721 cataatatgg ccatacgaac agatcttggc attccacccc aaaaagctct caggtagcct
     3781 ccaatccacc gaaaaaaccc agatccgacc gacctgctcc ccctgggccg tccaaacctc
     3841 ccacatcccg aggaggtccg tcggaaaacc acccacattt gttataggtt cgccaatccc
     3901 ctgctaaacg cccgtttcgc tgttcaactg caatatgtgc gcaaccgcgg cgcttcggcc
     3961 tctgtggcca ggcctatcca tggacaaggg taacatatag tactagctag gcctggcccg
     4021 atgtacgttc tgcaccccct ggagtcgctt gagcctgccg atcacattgt tcatgtgcgc
     4081 cacgtcgttt atctccacag tcatgttgat gagggccatc cggttccgca aggtcctggc
     4141 gttcaccgcg ctaacatgcg ccttcatctc cagcaccgcg ttcatgacgc ctgtaagcag
     4201 ccctgggctg tcgctggctt ccatctcgat ttctacagga taggacgtag cgttacccat
     4261 gtcccagctg acatctacct gcctgccttc gtcctccccc atcatggacg aaatgttggg
     4321 gcagtcctcc ctatgcaccg acactccgcg tccgcgagtg atatatccca caatcggatc
     4381 gcccgggacc ggattgcagc acctcgcgaa cctgaccagt acattgtcga tcccccggac
     4441 caccactcca tgggtggatc tagacgcccg cacggccccc ggaagcggtc gcgcaacctc
     4501 ttcaaacacc gactcagggc tcttctcggc ggccttctca ggaacaagct tgccaactac
     4561 cattgcggcc gacaacttgc cgtacccgat ggaggcgagc agatcatcgg cgctttggta
     4621 gctgaacttg cgtgcagcct cggcgaggcg ctcctctttg aggttggcat gcacctcatg
     4681 gcccatgcga cgaagatccc gttcgagcat ctccttgcct cgctcgatgg actcttcacg
     4741 tcgctgctcc ttgaaccact gcctgatctt gcccttggcc ttggaggtct tggcgatgtt
     4801 gagccagtcg cggctgggcc cggtctgcgc cttgttggtc acgatttcga cgatatcgcc
     4861 tgtggcgagc ggcgtcccaa gcgggaccat cctaccgttc acccgtgcgc ccgcgcacct
     4921 gttgccaatg tcggtgtgga ctgcataggc gaagtctatg ggggtagacc ctgccggaag
     4981 cgtcttcaca tcgcccttcg gcgtgaacac gtacacttcg tctgagaaca ggtcgacctt
     5041 gagcgtttca acaaactcgg taccattgtg cgtctctttt tgccagtcga ggatctggcg
     5101 cagccaggtg atcttgtctt cgaagtcacc gcccccgacg ccgccatctt tgtagcgcca
     5161 gtgggctgca atgccgtact ccgccgtgcg gtgcatttcc cacgttcgga tctgaatctc
     5221 gagcggttcg ccgcgcggcc cgataaccgt agtgtgcagc gactggtaca tgttggactt
     5281 gggcatagcc acgtagtcct taaaccgccc ggggattggc ttccatatgc tgtggaccgc
     5341 cccgagcacc gcgtagcatt cgcgcaacgt gcccacgatc acccgtagcc ccatcaggtc
     5401 ccatatctcg ctgaaatcct tgccctgctt gatcatcttc tggtagatgc tgtagaagtg
     5461 tttgggacgg ccctgaatct cggcggggac tccgatctcc tcaagggccg cggacagggt
     5521 ttgcctggct tcttctatat gcccctcccg ctcctgccgc ttggtagcta ccttctccac
     5581 cagctcatag tagacgccgg gttccagata ccgaagtgcc agatcctcga gttcccattt
     5641 gatcctccac atgcccagcc tgtgggcaag tggagcatat atctcgcaag tctcgccggc
     5701 gactcgcttt tgcttgtctg gctggcactc tctcagagtg cgcatgttgt ggagcctgtc
     5761 ggcaagcttg atcaggataa ccctgatatc cttggccatc gcgacgaaca tcttgcgcag
     5821 gctctcggcc tgctgctcct cgcgggtctt aacgtccatt cgggacagc
//
LOCUS       DAOTAC010000153         5830 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_11521_length_5830_cov_5.538528, whole genome shotgun sequence.
ACCESSION   DAOTAC010000153 DAOTAC010000000
VERSION     DAOTAC010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5830
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11521_length_5830_cov_5.538528"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            259..927
                     /locus_tag="PLM74_07420"
     CDS             259..927
                     /locus_tag="PLM74_07420"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HON42939.1"
                     /translation="MKEHLALFLEAADTASADIYLVREELASELERLSGQLLKDPGLG
                     SDPVENQEVCRRLSVLAQALYHPDRSEQATSGAASLRQASMNADVLARRIQFGVALRA
                     LRVRQGMRQKELALKAKIDPGYESRLERFLAGPPSQDVVQRLSAALGDVAGELPAAHQ
                     ATPAWHPSSAPRLKASAEYDDLLGQLLAVCAKLPLDHLRIILAQAQAVYALVASQKPD
                     RHVD"
     gene            1073..1894
                     /locus_tag="PLM74_07425"
     CDS             1073..1894
                     /locus_tag="PLM74_07425"
                     /inference="COORDINATES: protein motif:HMM:NF018290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HON42940.1"
                     /translation="MARVISILNIKGGVGKSTISFNLAHAIQRNNKRVLLVDLDMQCN
                     LTDMCVPEPERITYSVYDLLTNEDFLPTNAVYKTVVQGVDIIPADLRLFEVEQVINPL
                     TNPAAMLMLRNRLAVGLHDSYDYIIIDCRPEISLLTMNALIASDHYMVPVLCDRHSVR
                     GIDMTDSYVKSAKRVNPNLQEIGIVINALDRRKTNAMTIYNYLRKKLNERIMETVVGT
                     NAAIETAATHQQTIFQYDARQPGVRYFKQLAAEVMRKCGDPIAVEDAGDGEAEEN"
     gene            2667..3083
                     /locus_tag="PLM74_07430"
     CDS             2667..3083
                     /locus_tag="PLM74_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42941.1"
                     /translation="MTVAAWIGKGVTVETIQRYIDYIREHNRKRSKNKIENPTGWLFA
                     AVESGWMVDTETMRQTAASSQITQQIDTEEQRREAERQQIEARMAELGPERLEQIRQE
                     ELAKCQHDPGYRAQPSDRMRGYYVESLVHMRILAEA"
     gene            3404..>5830
                     /locus_tag="PLM74_07435"
     CDS             3404..>5830
                     /locus_tag="PLM74_07435"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019589398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HON42942.1"
                     /translation="MAIKKSALYSSIWSSCDELRGGMDASQYKDYVLVLLFMKYVSDK
                     HAAQKNALVEVPPGASYDDILALKGDKEIGDKINKIIARLAEANNLKGVIDQADFNDD
                     GKLGRGKEMVDRLSNLVAVFDKPELDFRGNRAEDDDLLGDAYEYLMKNFATESGKSKG
                     QFYTPAEVSRVMAQVIGVGEATSGGQTIYDPTCGSGSLLIKAHDEALARTGYDLALYG
                     QEMDNATHALARMNMILHGCPTAEIGKGNTLSNPLFTQGDASLKTFDFVVANPPFSNK
                     SWSNGLNPTEDLFGRFALGIPPKKNGDYAFLLHLLASMKSTGKGAIVMPHGVLFRGNA
                     EAEIRRNIVKRGYIKGIIGLPANLFYGTGIPACIVVLDKENAAVRRGVFMIDASRGFV
                     KDGNKNRLRAQDIHRIVSVFNSQAEVPGYSHMATFDEIEQNDFNLNIPRYIDSTEPED
                     LQDIDAHLSGGIPDRDVDGLAQYWAVFPSLRTELFEPADRPGYSQLKIPASSVRSTIF
                     GYPEFDAFNQSVTALFDGWRAAHVRKLKDLGPGHHPRELISELSESLLSVFRGTPLID
                     PYDVYQHLMTYWAETMQDDVYLIVSNGWREAARLRLMVDVKVGKDKEKPDITVGKQKY
                     KADLVPPALIIARYFPDAQAAIDELETRRVAIAQQMDELRDEQGGEEGLLSSVIDDGK
                     ITKGDLTTRLREIRGDAEFDDERAVLERYLGLMDEDSATAREIRDKRAELDAQLAGKY
                     ASLTEDEVKTLVVDEKWMGALAADVQFELHRISQTLGARIRELAERYGETLPELVNQV
                     TALSSRVDEHI"
BASE COUNT         1337 a         1665 c         1757 g         1071 t
ORIGIN      
        1 ggtggggccg caccctaatg aattgttctg cggagagggg ctcttttttc cgcttagggg
       61 gtgctggtcg gggagagcga gtttgccgct tcgctaccct tcctgtcagg gaagcattcc
      121 cccagcgagg ccttgatcat tcgccccttc tccgctagag tttgcatgga gagcaaatcc
      181 acgcactgcc atgcaaaggc gtgcccgctc attgtccgcg gtacaaatag ttgcgggctt
      241 ggcaatgggg gcgacgggat gaaagagcac ctggctctgt tccttgaggc tgccgatacg
      301 gcttccgccg acatctacct ggttcgagaa gagttggcct ccgaactgga gcggctctcg
      361 ggacagctgt tgaaagaccc cgggcttggc agcgaccctg tggagaacca ggaggtctgc
      421 cgtcgtctga gcgttctggc gcaggcccta taccaccccg atcggtcgga gcaggccact
      481 tccggcgccg ccagtctgcg gcaggcctca atgaacgcgg acgtgctggc gcgcaggatc
      541 cagttcggag tagcgttgag agcgctgcgc gtgcggcagg ggatgcgcca aaaggagctg
      601 gcgctgaaag ccaaaatcga ccccggttac gaaagccggc tggaaaggtt cctcgcgggc
      661 ccgccgtcgc aggacgtggt gcaacggctt tcggctgcgc tgggcgatgt ggctggcgaa
      721 cttccggcgg cgcaccaagc cacccccgca tggcacccct cgtctgcgcc aaggctgaag
      781 gcctcggcgg agtacgatga cctccttgga cagctcctag ccgtatgcgc caagttgccc
      841 ctagatcacc tgaggatcat cctggcccag gcccaggccg tctacgccct cgtggcgtcg
      901 cagaagcccg accgtcacgt cgattagccc atcgataccc ctttagcccc cgcccctatg
      961 atctcccgat ttttctcttg ccaaaggagg aactacacgg cttacgcaga aggactcagg
     1021 gcatggtaag cctgaatgtt ttcgactata tgtttgaatg aggggatgca acgtggctcg
     1081 tgtaatctct attctgaaca tcaaaggcgg tgtaggtaag agcacaatca gctttaacct
     1141 ggcccacgcg atccaacgta acaataagcg cgtccttctc gtcgacttgg acatgcagtg
     1201 caatcttaca gacatgtgcg tacctgagcc cgagcgtatc acatattccg tctatgacct
     1261 tctaactaac gaggacttcc tgcccaccaa tgccgtctac aagactgtgg tacagggtgt
     1321 ggacatcata ccagctgatt tgcggttgtt tgaggtcgag caggttataa accctctaac
     1381 aaatccggct gcaatgctga tgttacgcaa caggctagcg gtaggattac atgatagcta
     1441 tgactacatc atcattgatt gtcgccctga gatatctctc ctcaccatga acgccctcat
     1501 tgccagcgat cattacatgg tgccggttct ctgcgacagg cattcggtgc gcggcatcga
     1561 tatgacggac tcgtatgtca agtcagccaa gcgtgtgaac cccaacctgc aggagatcgg
     1621 catcgttatc aacgccctgg atcgccggaa gaccaatgct atgaccatct ataactacct
     1681 gaggaagaag ctcaatgaac gcatcatgga gacggtggtc ggcacgaacg ctgcaatcga
     1741 aaccgcagcc actcaccaac agaccatttt tcagtatgac gcccggcaac ctggggtgcg
     1801 ctacttcaaa caactggcag cagaagtcat gcggaagtgc ggcgatccca tagcggtgga
     1861 ggatgcaggc gatggcgaag ccgaagaaaa ctgaggtaac tattgactct ctactggatc
     1921 gctacataga tcctgtggtg gagcgtctgg agaaagaaaa ggccgagact catggtagtc
     1981 cctcagaggg tagtccctca cagggaggtc cctccacggg tagtcccaac gagggatgtc
     2041 cctcacaggg acccctagca aatagtctcc ccccagagag tgatcactcc gatggcacac
     2101 ccgtgcctgt tgctcctgtc ttgccaccag aacgctcctt cactagggct gcgaactggc
     2161 tgtgggacat gttaccgcag cacatcccct cgctatgcga ctgggcgatc tatacctact
     2221 tgtatcgcct ctcgataggc tacaaccaga cttcatgcgt agttagtcga tcaacgatac
     2281 agaagagcct agggctcggg cgcaacacag tgatccggtc tatcgcctct ctcaaaaaac
     2341 gtggcttcat aacggagggt ttgacaaccc aggagggaac agagatcact ctcttcgtgc
     2401 ccatagaggt gctaggtagt ccctcacagg gacctccctc agagggtagt ccctcacagg
     2461 gacctccccc aaggggaccg gcaggtagtc cctcacaggg acctccctca aagggacctc
     2521 ctaagaaagg ccagctaaag tcggggggtc cctcacaggg acctccctca aagggacaca
     2581 taaaagacag tagtactttt aaaaacaaca acaacagaca agacgttgtt gctgttcttc
     2641 aagaagcagg ttacgctatt gaccccgtga cggtggctgc atggatcggg aagggagtaa
     2701 ccgtcgagac gatccagcgc tatatagact acatccgcga gcacaacaga aagaggagca
     2761 agaacaagat cgagaaccct acggggtggc ttttcgccgc tgtggaatct ggctggatgg
     2821 tggatactga aaccatgcgg cagacggctg ccagcagcca gataacacag cagatagaca
     2881 cggaagagca gcgcagggag gccgagaggc agcagataga ggcccgcatg gccgagctgg
     2941 gccccgagcg cctggagcag atcaggcagg aggagcttgc caagtgccag cacgaccctg
     3001 gctaccgtgc ccagcccagc gacaggatga gggggtacta cgtggaatcg cttgtccaca
     3061 tgcggatcct ggctgaggcg tagggtgtgg atacttggtc aagaagacaa ctgtgggatg
     3121 aagtaaacgt ttagaaagtg gacgtccatg atctatacgt ttaggagatg aacgcataca
     3181 aaccgattct cccagcgcgg cgcgatcgga gctgcgtccc tggaggcgct tgccgtgaaa
     3241 agtggaggag atctggagcg ttatgagaat acaccacaca taagcaggct tgcccgtcta
     3301 gggcggagcc aaacacaaca atccagccgg tgccgatgcg tgcccggctt tcagcgatag
     3361 accggacgcc gcggggtgcg ttaacggcgc taggaggtgc catatggcga tcaagaagtc
     3421 cgcgctttac agttcgattt ggagcagctg cgacgagctc cggggcggga tggacgcctc
     3481 acagtataag gactacgtgc ttgtcttgct gttcatgaag tacgtgtccg acaagcacgc
     3541 cgcccagaag aacgcgctgg tggaggtgcc gccgggcgcg agctacgacg acatcctggc
     3601 gctgaagggc gataaggaga tcggcgacaa gatcaacaag atcattgcca ggctggccga
     3661 agccaacaac ctcaaggggg tcatcgacca agccgacttc aacgacgacg gcaagctggg
     3721 ccggggcaag gagatggtcg accgcctgtc caatctcgtg gccgtgtttg acaagcccga
     3781 gctggacttc cggggcaacc gggcggagga cgacgatctc ctgggcgatg cgtacgagta
     3841 cctcatgaag aacttcgcca ccgagtcggg caagagcaag gggcagttct acaccccggc
     3901 ggaggtctcc cgagtgatgg cccaagtcat tggcgtgggc gaggccacca gcggcggcca
     3961 gacgatctac gacccgacgt gcgggtcggg ttccctcctg atcaaggccc acgacgaggc
     4021 cctcgccaga accggctacg acctcgccct ctacgggcag gagatggaca acgccaccca
     4081 cgcgctggcc aggatgaaca tgatcctaca cgggtgcccc acggctgaga taggcaaggg
     4141 caacacccta tcgaatccgc tgttcacgca gggtgacgcc agccttaaga ccttcgactt
     4201 cgtggttgcc aatcccccgt tctcgaacaa gtcgtggagt aacggcctga acccgactga
     4261 agacttgttt ggtcgctttg cgctcggtat tccacccaag aagaacggcg actacgcatt
     4321 ccttctccac ctgctggcat cgatgaagag caccggaaaa ggggcgattg taatgcctca
     4381 cggcgtccta ttccggggca acgccgaggc cgagatccgc aggaacatcg tgaagcgtgg
     4441 ctacatcaag gggatcattg ggttgccggc caacctgttc tacggcacag gcatccccgc
     4501 ctgcatcgtg gtgctcgaca aggagaacgc cgccgttcgc aggggtgttt tcatgatcga
     4561 cgcgagccgc gggttcgtga aggacggcaa caagaaccgg ctgagggcgc aggacattca
     4621 cagaatcgtc agcgtcttca acagccaggc ggaggttccg ggctactccc acatggccac
     4681 cttcgacgag atagagcaga acgacttcaa cctcaacatc ccacgctaca tagacagcac
     4741 cgagcccgaa gacctgcagg atatcgacgc gcacttaagc ggcggcattc ccgaccgcga
     4801 cgtagacggc ctggcgcagt actgggcagt gttcccgagc ctgcgcaccg agctgttcga
     4861 accggctgac aggccggggt acagccaact caagatcccg gccagcagcg tcaggtctac
     4921 gatctttggc tatcccgagt tcgacgcgtt caaccagtcg gtgacggccc tgtttgacgg
     4981 gtggcgcgct gcacacgtac ggaagctgaa ggacctgggc ccgggacatc accctaggga
     5041 gctcataagc gaactgtcgg agagccttct gagcgtgttc cgcgggacgc ctctgatcga
     5101 cccctacgat gtgtaccaac acctgatgac ctactgggcg gagacgatgc aggacgacgt
     5161 ttatttgatc gttagcaatg ggtggcggga ggctgcccgg ctgcggctca tggtcgacgt
     5221 caaggtgggt aaggacaagg aaaagcccga catcacggtg ggcaagcaga agtacaaggc
     5281 agacctggta cctccggcgc tgataattgc ccgctacttc cccgacgctc aggccgcgat
     5341 cgacgaactc gagaccaggc gtgtcgccat tgcccagcag atggacgagc tcagagacga
     5401 gcagggcggg gaagaagggc ttctgtcctc ggttatcgat gacggcaaga tcaccaaggg
     5461 cgatctgacg actcggctca gagagatcag gggcgatgcg gagttcgatg acgaaagggc
     5521 tgtgctggag cggtatctgg gcctgatgga tgaggactcg gccacggcca gagagatcag
     5581 ggataagcgg gccgagctag acgcgcagct agcgggcaaa tacgcaagct tgactgaaga
     5641 tgaggtcaag acgctggtgg tcgacgagaa gtggatgggc gcactggctg ccgatgtgca
     5701 gtttgagcta catcgcatat cgcagacgct gggtgcccgt attcgcgagt tggcagaacg
     5761 ctacggggag acgctccccg agctcgtaaa tcaggtcacg gccttgtcct ctcgcgttga
     5821 cgagcacatc
//
LOCUS       DAOTAC010000154         5830 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_11522_length_5830_cov_4.812987, whole genome shotgun sequence.
ACCESSION   DAOTAC010000154 DAOTAC010000000
VERSION     DAOTAC010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5830
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11522_length_5830_cov_4.812987"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="PLM74_07440"
     CDS             complement(<1..676)
                     /locus_tag="PLM74_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42943.1"
                     /translation="MAAIAKYFHVARVSMRARTAYLADFLLGSVFYALILYVFVQLWS
                     VIGRTRGFVVEGFGPRELVWYLMASEAIMLGRARFEQETDEEVKTGAIAYSLNKPYHY
                     VMFKLASYWGETALKMALNIVIGSAVALAAVGPIPVSIPHLAAVLLCTALALTLNFFI
                     GMVLGLSAFWVEDTAPFFWIYSKVLFVLGGLFAPMEIYPDALARVARATPFNYVLYAP
                     ARLFVQF"
     gene            complement(681..1682)
                     /locus_tag="PLM74_07445"
     CDS             complement(681..1682)
                     /locus_tag="PLM74_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015256086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HON42944.1"
                     /translation="MATIDVCGLSKVFEVREKAPGLAGSLRSIVSPSVREVEAVREIS
                     FSVDEGEMLAFIGPNGAGKSTTIKMLTGILHPTGGQASVLGVTPWEDRQRLAGEIGCV
                     FGQRSQLWYHLPPRDTFELLAHVYEIPKPVYRDRLQMLASRFGLDEFMCTPVRKLSLG
                     QRMRCEIAASLLHGPRVLFLDEPTIGLDVVAKRTIRELIRQMNREEGITVFLTSHDVG
                     DMEELCRRVMIINHGELILDTSVSALRRDYLRFKIVRVRLADAIDGFEMDGVAVVKHK
                     RTNLKLSVDTDLTSIDEVLAGLMRRTRIVDITIADPPTEQVIADIYGRGRSEALEGR"
     gene            1762..1968
                     /locus_tag="PLM74_07450"
     CDS             1762..1968
                     /locus_tag="PLM74_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42945.1"
                     /translation="MERKHRSTSRKLTPTGSNTSTSFKCAQCGTMYPAGEEMGASGCM
                     VCPSCGFENCTDDSTIVNASDEEY"
     gene            complement(2099..2779)
                     /locus_tag="PLM74_07455"
     CDS             complement(2099..2779)
                     /locus_tag="PLM74_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42946.1"
                     /translation="MKRPVLTVFLFIIVFIVCIAVPPAPQADAATGGYEVISVEPMMH
                     GGMHIIFERTAAGASTGSKWHVTVQSVNYPKWVEISWVWPRSDGTLPAATIRRINGLE
                     RGRTFGPVFGVRDPVDTDSTAPWVSREVLRELRTGKVAYNFKMGSVSLTGILAGDLKV
                     EEEVLFPVDLNGRRVFLPAYRCAKGQLTIWNNMQNPLVLEYRPIGVPLITGVFGWKAE
                     SISIGGEV"
     gene            complement(2824..4416)
                     /locus_tag="PLM74_07460"
     CDS             complement(2824..4416)
                     /locus_tag="PLM74_07460"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HON42947.1"
                     /translation="MGRYLFTGGKVYTASGRRPFCQAIACVGKRVIAVGSESEVAAMV
                     DDSFERIDLAGRALVPAFTDSHIHLAHYALSLANVELDGASGPEEAATRVARAVEGAR
                     SGSWIVGQGWNKNTWPGGALPDRSYLDSVAQRNPVALFSKDLHSLWVNTAALRIAGIG
                     LDTPNPEGGEIVRDDDTRQATGILKEAATALIRRVIPEPTCEAMAAAMEKAQSELHKL
                     GIGAVHNMDGADSFEALQLLRNTGRLKIRVTQSIAKDNLDDAIDLGLSSGFGDEWLRV
                     GPLKAFADGALGSQTAHLLRPYEGNSRNTGLAVASKEEMAELVGKAVLAGICCAIHAI
                     GDRANRDVLDIFESVKAESAQRRLRHRIEHAQLLHPEDVRRFADLGVVASMQPVHILT
                     DIPVAERHWGRRSRWAYAFRSLSKSGAMLAFGSDAPVETPDPIRGVYAAVARKQLGGT
                     PDSGWYPEERMTVEETIRAYTAGAAMASADELSRGTLARGSFADMTVLSRDIFSTPAA
                     EILDVRVAATVIAGQFVFREGV"
     gene            4592..5443
                     /locus_tag="PLM74_07465"
     CDS             4592..5443
                     /locus_tag="PLM74_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HON42948.1"
                     /translation="MPISRILVVGAGQMGAGIAQVAAQAQIAVTLTDISVDYARRGLD
                     RISKALSKLVDKGRMAPEQRDSILSQIKVAPDLEVAAQGADLVIEAIVEEPTAKSELW
                     SRLADICQPDVIFASNTSSISITSMARDSRRADRFIGMHFMNPVPIMQLVEVVRGLET
                     SDSVCDTIVELARKMGKEPVVVSDSAAFVVNRIAIPMINEAAFALMEGVSTAEGIDTC
                     AKLGLNHPMGPLALADLIGLDTVVSILEVMVAETGDPKYRPCPLLRRMVAAGRLGRKA
                     GQGFYKY"
     gene            5489..>5830
                     /locus_tag="PLM74_07470"
     CDS             5489..>5830
                     /locus_tag="PLM74_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004826540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HON42949.1"
                     /translation="MRSDSVKLAVSDYTAVITISRPSALNALSSAVLAELDAALAEVR
                     ASGDCRALIITGEGEKAFVAGADIGEMRDLGPDEAADFARRGQAVFRAIEELPIPVIA
                     AVNGYALGGGCE"
BASE COUNT         1231 a         1857 c         1656 g         1086 t
ORIGIN      
        1 cgaactggac gaacaggcgc gccggcgcat acagcacata gttgaaagga gtcgcccgcg
       61 ccacccgcgc gagggcatct gggtagattt ccatgggagc gaatagcccg cccaggacga
      121 aaaggacctt gctgtatatc cagaaaaacg gggccgtatc ctcaacccag aaggcggaaa
      181 ggcctaaaac catgccgatg aagaagttga gcgtcagggc cagcgctgtg cataacaaca
      241 ctgcagctag atgcgggatg ctcacgggaa tgggccccac cgccgccagg gccacggccg
      301 agccgatcac aatgttcagc gccattttga gggcagtctc tccccagtag gatgccagtt
      361 tgaacatgac gtaatggtaa ggcttgttga gagagtaagc tatggcccca gtcttcactt
      421 cttcatcagt ctcctgctcg aatcgagcac ggcccagcat gattgcctcg ctggccatca
      481 ggtaccacac cagctcgcga gggccgaagc cttccactac aaagcctcgc gtgcgcccga
      541 taaccgacca taactgcacg aacacataca aaatcagcgc atagaacacc gaacccagca
      601 agaagtccgc caggtacgca gttcgcgccc gcatgcttac tcgggcgaca tggaagtatt
      661 tcgcgatcgc agccaccgcc tcatcttcct tccaacgctt ccgacctacc gcgcccgtaa
      721 atatcggcga taacctgctc cgtaggaggg tcggcaatcg tgatatcgac tatccgcgta
      781 cggcgcatca agccggcgag cacttcatcg atggaagtca ggtcggtatc caccgacagc
      841 ttaaggttcg tccgcttatg cttaaccacg gcaacaccat ccatctcaaa tccgtcgatg
      901 gcatcggcaa gcctgacacg cacgatcttg aagcgcaggt aatcgcgcct caaggccgac
      961 acggatgtat ccaggatcag ctcgccgtgg tttatgatca tcacgcgcct gcacaactcc
     1021 tccatatcgc ctacatcgtg cgacgtcagg aacactgtga ttccctcttc ccggttcatc
     1081 tggcgaatca gttcccggat agtgcgcttg gccactacat ccaggccgat agtgggttcg
     1141 tccaggaata gcaccctcgg cccatgaagc agtgaagcgg cgatctcgca cctcatgcgt
     1201 tggcccagcg aaagcttgcg gacgggagtg cacatgaatt catccagccc aaacctggat
     1261 gccagcattt ggagccggtc acggtagacg ggctttggga tctcgtacac gtgtgccaga
     1321 agctcgaacg tatcgcgcgg cggcaaatgg taccacagct gcgatcgctg gccgaacacg
     1381 cacccgatct cgcccgccaa tcgctgcctg tcctcccacg gagtgactcc caggaccgac
     1441 gcctgtcctc cagtggggtg caggattccg gtgagcatct tgatcgtagt tgacttgccg
     1501 gccccattgg ggcctatgaa cgccagcatc tcgccttcgt ccaccgaaaa cgatatctcc
     1561 cgcactgcct caacttcgcg cacagacggc gagacgatcg aacgtaggct gcctgccagg
     1621 ccaggtgcct tttcgcgcac ctcgaacacc ttggatagcc cacaaacgtc gatggttgcc
     1681 acattttcca cccgcctcat gagaatgcta ctttaggcgc ctcgcgccgg tgatcacttc
     1741 gcgacatcgg gaggtagaag agtggaacgg aaacacagat ctacaagccg gaaactgacg
     1801 cccactgggt cgaatacgtc tacaagcttt aagtgcgccc agtgcggcac catgtaccct
     1861 gccggggaag aaatgggcgc atcgggatgc atggtctgcc ccagctgcgg cttcgaaaac
     1921 tgcacggacg attccacaat cgtgaacgca tccgacgaag agtactaggc tgcaggaagc
     1981 agagagtcgc aggcagttgt aggcagttgt aggcagttgt aggtagttgc agcatcgccc
     2041 gggccgatcg gacctgcccg ggcgtctgta ttcagtgccg gcattcagct acacagccct
     2101 atacctctcc cccgatcgat atggactctg ccttccagcc aaacaccccg gtgatcaggg
     2161 gcacgccaat cggtcggtac tccagcacca gcgggttttg catgttgttc caaatagtca
     2221 gctgcccctt ggcgcacctg tacgcgggca ggaataccct cctcccgtta aggtccacag
     2281 ggaagagcac ttcctcctct accttcagat ccccggccag aatccccgtg aggctgacgc
     2341 tgcccatctt gaagttgtac gccactttgc cagtgcgtag ctcgcgcagg acctcccgcg
     2401 acacccaagg cgcagtgcta tcagtgtcca ccggatctct cacgccgaat actggaccga
     2461 acgtgcgccc tctctcaagc ccgttgatgc gcctgatggt ggcagcaggg agcgtgccgt
     2521 cggatctggg ccacacccag ctaatctcga cccatttggg gtagttgacc gattgcacag
     2581 tcacatgcca cttgctgccg gtggaagcgc cggccgcggt gcgttcaaag atgatgtgca
     2641 ttcccccatg catcataggt tcaacgctga tcacctcgta tccgccagtg gcggcgtcag
     2701 cctgcggagc cggcggaacc gcaatacaca caatgaagac aatgatgaac agaaaaaccg
     2761 tgaggaccgg tcgtttcaag ccgaactccc ctttccgcaa atatcgctat agccgcgtgc
     2821 ggcctagact ccctctctga acacaaactg cccggcgata accgtagccg ccacacgcac
     2881 gtccagaatc tcagccgcgg gcgtcgagaa gatgtcgcgc gacaataccg tcatgtccgc
     2941 aaacgagcca cgagcgagcg ttccccgact cagctcgtcg gctgacgcca tggctgcgcc
     3001 ggctgtgtat gccctgattg tctcctcaac cgtcatccgc tcctctggat accatcccga
     3061 atcgggagtg ccgcccagct gtttcctggc cacagcggcg tagacaccgc ggataggatc
     3121 gggagtctca acaggcgcgt ccgaaccgaa agccaacata gcgccgctct ttgacaggct
     3181 gcggaaggcg tatgcccacc ggctacgccg accccagtga cgctcggcca ccggtatgtc
     3241 ggtcaatatg tgaaccggct gcatcgaagc taccacgcca aggtctgcaa accgccgcac
     3301 atcctcggga tgaagcagct gagcatgctc gatcctgtgg cgcagccgac gctgcgcgct
     3361 ctcggccttc accgattcga atatgtcgag cacatcgcgg ttggcccggt cgccgatggc
     3421 gtgaatcgca cagcaaatcc ccgcgagcac ggccttgccc actagctctg ccatctcctc
     3481 tttggacgct acagctagcc ctgtgttcct agagttcccc tcatacgggc ggagcagatg
     3541 ggccgtttgg gatcctaaag caccgtctgc aaatgccttg agcggcccga cgcggagcca
     3601 ttcgtcgccg aatccgcttg aaagccccag gtcaatggcg tcatccagat tatccttggc
     3661 tatggactgg gtgacacgaa tcttcaggcg cccggtgttg cgcagaagtt gcagagcctc
     3721 aaaggagtcg gcgccgtcca tgttgtgaac cgcccctatc cccagcttgt gcagctcaga
     3781 ctgggccttc tccatcgctg ccgccatggc ttcgcaggtc ggctcgggga tcaccctcct
     3841 gattagggcg gtcgcggctt ccttcaggat ccccgtggcc tgacgggtat catcgtccct
     3901 cacgatctcg ccgccttcag ggttcggcgt gtcgaggcca atcccggcta tacgcagcgc
     3961 agccgtgttg acccacagcg agtgaaggtc cttgctgaac aaagcgaccg ggttgcgctg
     4021 cgcgacggag tctaggtatg acctgtcggg cagagcgccg ccgggccacg tattcttgtt
     4081 ccacccctga cctactatcc atgaaccgga cctggcgcct tcaacagctc gcgccacgcg
     4141 cgtcgcagcc tcctcaggcc ccgacgcacc atccagttcc acattggcca ggctcagagc
     4201 gtagtgcgcc agatgaatgt gcgaatcagt gaacgcggga accagggccc gcccagcaag
     4261 atcgatcctt tcgaagctat cgtccaccat cgcggcgact tccgattcgg acccgaccgc
     4321 gatgacccgc ttccccacac acgcgatagc ctggcagaaa ggtcggcgtc ccgacgccgt
     4381 gtacaccttt ccgcccgtga acaagtacct gcccacaacg gtccccccag tcttacacag
     4441 ctgattacgt attccatcaa ccacccccct tttcctgccg ggcccggcgg agcaggagtc
     4501 aaccacgagt tatcgaagta aagaaggcgc aagcgcgcag aaagcgacgc gccttcagga
     4561 gccggatcga tgagcgaaag gtggttgcga catgccgatc agcagaatcc tcgttgtagg
     4621 ggccgggcag atgggcgccg gcatcgcaca ggtggcggcc caagcccaaa tcgccgtgac
     4681 tctgaccgat atctcagttg actatgcccg gcgaggcctt gaccgcatat cgaaagcctt
     4741 gagcaagctg gtagacaaag gacgtatggc gccggaacag agggactcta tcctctcgca
     4801 gatcaaggtg gcgccggatc tggaggttgc tgcccagggc gcagaccttg tgatcgaagc
     4861 catcgtggag gagccgacgg ccaagtcgga gctatggagc agactggccg acatctgcca
     4921 accggatgtc atcttcgcat ccaacacttc gtccatttcg atcacgtcaa tggcacgcga
     4981 ttcacgccgc gccgatcggt tcataggcat gcacttcatg aaccccgtcc ccatcatgca
     5041 gctggttgag gtagttcgcg ggctcgagac atccgactca gtatgcgaca caatcgtgga
     5101 gcttgcgcgg aaaatgggca aggagccggt ggtggtatcg gattcggccg cgttcgtagt
     5161 caacaggatc gcgatcccca tgatcaatga agccgccttt gccctcatgg aaggggtatc
     5221 cacagccgag ggcatcgata cctgcgcaaa gctcgggctc aaccacccta tgggcccgct
     5281 ggcccttgcg gacctgatcg gcttggatac agtagtgtcg attctggaag tgatggtggc
     5341 ggagacgggc gaccccaagt accgcccctg cccgctcctg cgccgcatgg tggccgccgg
     5401 caggctgggc cgcaaggcag gccaggggtt ctacaagtat tgacgagcca cgcgaccgag
     5461 tgatcatgga agcgaggagg tgcggcgttt gagaagtgac agcgtcaagc tagctgtttc
     5521 cgactacacg gcggttatca ccatatcgag gccatccgcg ctcaacgcac tcagcagcgc
     5581 ggttctggcc gaactcgatg cggcgctggc cgaggtgcgc gcaagtggcg actgccgtgc
     5641 gctgataatc accggggagg gcgaaaaagc ctttgtggcc ggcgccgaca tcggcgagat
     5701 gcgcgatctg ggcccggatg aagcggcgga tttcgctcga cgtgggcagg ctgtgtttcg
     5761 cgccattgag gagttgccca ttccggtgat cgccgccgtc aacggttacg cactgggcgg
     5821 cggatgtgaa
//
LOCUS       DAOTAC010000155         5790 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_11625_length_5790_cov_4.649520, whole genome shotgun sequence.
ACCESSION   DAOTAC010000155 DAOTAC010000000
VERSION     DAOTAC010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5790
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11625_length_5790_cov_4.649520"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..2237)
                     /gene="glyS"
                     /locus_tag="PLM74_07475"
     CDS             complement(111..2237)
                     /gene="glyS"
                     /locus_tag="PLM74_07475"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HON42950.1"
                     /translation="MAISEKGPMRDAVLEIGVEELPARFMRQALADARTYITDALASA
                     RLEASEVCVYGTPRRIIVLLRQVAPRQNDLVREVRGPAARAAYDSEGRPTRAAMGFAR
                     SVGAAVEELVSRPTPQGDYVYATVTEGGRPAGAVLAEVFPQTIAAMSFPKPMRWGSHD
                     FRFARPIRWILALLGAEVIPFDTHAVSSGRATWCHRTLAPGAHEIESAEEYLPRLREL
                     GVMVDPEERAESIRRQAESLTSSIGGQAVIDPELLEEVTFLVEWPRAFVGRFAEDYLK
                     LPEACVITPMKDHQRYFPVRDGRGNLMPVFVGVRNGGDLGIDIVTAGNEKVLAARLAD
                     ARFFYEEDMKRPLAERVDDLRGVVFQERLGTMYEKAVRVGRLASDLLRGAPEAALVER
                     TALLAKADLVTSVVREFTELQGVMGREYALRQGEDMAVADAIFEHYLPRFAGDILPAS
                     LLGAALSIADKIDTLVGYFSIGLVPSGSADPYALRRQAAGAVLVHTEWRFQVGLDSLA
                     ARAASLFGKDGAAEDGAPRAQVVQDVLAFVRARLKASLEEAGLRHDIADAVLAAGFGH
                     PACVFERAHAVAQVVDESWFAALATAGSRVRNIAMKAETDAFSPALFQDDAERELFDA
                     AKSMEADVRASIEARMSGFVDRTAYVSALERMSVVCPVIDKYFEKVMVMVDDEAVRNN
                     RLAMLKWVSGVLSMVIDLSTIVFAGG"
     gene            complement(2224..3093)
                     /locus_tag="PLM74_07480"
     CDS             complement(2224..3093)
                     /locus_tag="PLM74_07480"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019542044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HON42951.1"
                     /translation="MNFQDLILTMERYWAQRDVTIQQPYVVEVGAGTMSPHTFLRVLG
                     PQPWSVAYVQPSRRPADGRYGDNPNRLFQHHQYQVILKPSPDNVQDLYLGSLAAIGID
                     PAEHDIRFVEDNWEAPTLGAWGTGWEVWLDGMEITQFTYFQQVGGMEVRPVSAEITYG
                     LERIAMYLQHVDNVYDLKWVGDLTYRDVYHQAEYEFSKYAFEVADIGVLRASFENYER
                     EASRCIHQNLVLPAYDYVLKCSHVFNILDARGAFGTAQRAEYMGRVRSLARLVARAWV
                     DRGHEEAADNGDK"
     gene            complement(3146..3790)
                     /locus_tag="PLM74_07485"
     CDS             complement(3146..3790)
                     /locus_tag="PLM74_07485"
                     /inference="COORDINATES: protein motif:HMM:NF014052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HON42952.1"
                     /translation="MLKRLSVLYTRALPRALRGYRRYFLFACVLFATCVVVGYRDYQI
                     QGDQLPWMFRNLAALRDSMEQKSRVMQILMLFWNNLRVSLISLLLGWILGIVPMLSVG
                     LNGVLIGVGTRLMVERGGWPLGIVIASFIPHGILELPAFIAVQAVGMRLGFLIPLVWR
                     KRATTGDLGRAFAEGVVIVLLFAAPALILAAVVELSLTPALIRAMAPGYPALPY"
     gene            complement(3946..4356)
                     /locus_tag="PLM74_07490"
     CDS             complement(3946..4356)
                     /locus_tag="PLM74_07490"
                     /inference="COORDINATES: protein motif:HMM:NF025601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HON42953.1"
                     /translation="MKIDLATVDRLRERVDLTYEEATAYLEAAGGDLVQALVMAERDL
                     GSQDGEGGPDEEGWVAAGAEARAATYSRLSERIADAVRQGSSMRLIIERDGDRLANLP
                     VAAGLVGVIWAPELAAVGAIAALASRCTLRLERC"
     gene            complement(4356..5126)
                     /gene="recO"
                     /locus_tag="PLM74_07495"
     CDS             complement(4356..5126)
                     /gene="recO"
                     /locus_tag="PLM74_07495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HON42954.1"
                     /translation="MALVRCEGIVLKVKDFGEADRILTVLSGSEGKFEALARGARRPR
                     SRLVGVTQQFAQVNLLLFRGRNLDTISSAELVSAHSSLWSDVARMAYASYMAELVDKM
                     TGERERNDQLYSLVVAAFDALDSGAVAPRIASLAFEIKFMATSGFLPELGQCVLCGAP
                     TGQSVWFGPEAGGLVCGRCRPTLRDAVSFGPAARETARLLLALTYERVGVLKPREDTL
                     DELETVFRAYVDHRVERKLHSLDFIRDLIKMEQEGGQG"
     gene            complement(5194..5730)
                     /locus_tag="PLM74_07500"
     CDS             complement(5194..5730)
                     /locus_tag="PLM74_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HON42955.1"
                     /translation="MKLLLHSCCAPCTTFCLKSLRADGHEVSGYFFNPNIHPYTEFAR
                     RLDTFKAYCSAVGHSAIIDETYGLRQFLGEIDGNVDGRCAECYRMRLYPAAKLARELG
                     ADGFTTTLLISPYQNHELLTRVAKEAGEAAGVPFAYYDFRTGYRESVQMSREMGLYRQ
                     PYCGCIFSEEERYSPARR"
BASE COUNT         1199 a         1886 c         1572 g         1133 t
ORIGIN      
        1 agtgccatga caatcctcct gtccgttgct gctctctact tgccaagtcg cctaagattc
       61 tactctactg agagctgtcg ttcaagtgag attgataatc gccctcataa tcatccgcct
      121 gcgaagacga tagtggagag gtcgatgacc atggataaca cgcccgaaac ccacttgagc
      181 atcgctagac gattgttacg cacagcttcg tcgtccacca tgaccatgac cttttcgaag
      241 tacttatcga ttacggggca taccacgctc attcgttcga gagcggatac atatgccgtt
      301 ctgtccacaa atccggacat tcgggcttca attgacgcgc gtacgtctgc ctccatagac
      361 ttagctgcat cgaacaactc acgttcagcg tcgtcctgga aaagcgccgg gctgaatgca
      421 tcagtctcag ccttcatcgc aatgttgcgc accctggacc ccgccgtggc aagcgccgca
      481 aaccagcttt catcaactac ctgcgcaact gcgtgagccc gttcgaatac acatgcggga
      541 tgaccgaagc ctgcggcaag cactgcgtca gcgatgtcgt gcctaagccc cgcctcctcc
      601 agagaagcct tgagcctggc ccggacaaaa gccaagacat cctggactac ttgcgcccgc
      661 ggagcaccgt cttctgccgc accatccttc ccaaacaagc tggccgcgcg ggccgccagg
      721 gaatcaagcc ccacttggaa cctccactcg gtgtgcacca atactgcacc tgccgcctga
      781 cggcgcaggg cgtacgggtc ggcggagccg gatggaacca gacctatgga gaagtatccc
      841 accagcgtgt cgattttgtc ggcgatggag agcgccgccc caaggagcga tgccggaaga
      901 atatcgcctg cgaaccgcgg taggtaatgc tcgaatatgg cgtctgcgac ggccatatct
      961 tcaccctgcc ggagcgcata ttcgcggccc ataaccccct gcaattcagt gaactcgcga
     1021 accaccgaag taaccaggtc agctttggcc agaagagcgg ttctctccac gagcgcagcc
     1081 tccggggcgc cgcgcagcag atccgacgct aagcgcccca ctcgcacggc cttctcgtac
     1141 attgttccca gtctctcctg gaaaactacg ccgcgcaggt cgtctactct ctcggcaagc
     1201 ggacgtttca tgtcctcttc gtagaaaaac cgcgcgtcgg caagccttgc cgccaacacc
     1261 ttctcgttcc cagcagtgac aatatcgatg cctagatcgc cgccgttgcg caccccgacg
     1321 aagaccggca tgaggttgcc ccgcccgtcg cgcaccggga agtagcgctg gtgatctttc
     1381 atgggcgtga tgacgcacgc ttcaggcagc ttgagatagt cctcggcaaa cctacccacg
     1441 aacgccctgg gccattccac cagaaacgtc acttcttcca acagctcagg atcgatcact
     1501 gcctggcctc cgatggacga cgtcaagctc tccgcctgcc ggcgtatgct ctcagcacgt
     1561 tcttcggggt cgaccatcac tcccaactcg cgcaggcggg gcaggtattc ctcagccgat
     1621 tcgatttcgt gcgctcccgg agcaagcgtt cggtgacacc acgtggcgcg accgctcgac
     1681 acggcatgtg tatcgaatgg gatgacttcg gcgccaagca gtgccagaat ccatctgatc
     1741 gggcgggcaa agcggaagtc gtgtgagccc cacctcatgg gctttgggaa gctcatggct
     1801 gcgatcgtct gtgggaagac ctccgccagc actgcgccgg ccggccgacc tccctcggta
     1861 accgtggcgt atacatagtc gccctgagga gttggcctcg acaccagctc ttcgacggca
     1921 gcacccaccg atctggcaaa ccccatcgca gctcgagtag gcctgccctc ggaatcgtag
     1981 gcagccctgg cggcaggccc cctgacctcc cgtaccaggt cgttctgcct gggtgccacc
     2041 tggcgcagca ggacgatgat acgccgtggc gttccgtaca cgcatacttc cgaagcctca
     2101 agcctggccg aggccagcgc atctgtgatg tacgtgcgtg cgtcggctaa tgcctgccgc
     2161 atgaagcgag ccggcaactc ctcgacaccg atctccaaga cggcgtctct catgggcccc
     2221 ttctcactta tcgccattgt ctgccgcctc ctcatgccct cggtcgaccc aggcgcgcgc
     2281 aacaaggcgc gcgagggatc tcactctacc catgtattct gcgcgctgcg ctgtgccaaa
     2341 cgctcctctt gcgtcgagta tgttgaaaac atgggagcac ttcagcacgt aatcatatgc
     2401 cggcaagacc aggttctgat ggatgcacct ggatgcttct ctttcgtagt tttcgaatga
     2461 tgcccgcagg acgccgatgt ccgcgacttc aaaggcgtat ttggagaact catattcagc
     2521 ctgatggtaa acgtctcggt acgtcaaatc gcccacccat ttcaggtcgt acacgttgtc
     2581 gacatgctga aggtacatgg cgattcgttc gagaccgtag gtgatctctg ccgatacggg
     2641 gcggacttcc atcccgccga cctgctggaa gtaggtgaac tgagtgatct ccatcccgtc
     2701 cagccacacc tcccagcctg tcccccatgc tcccagcgtg ggcgcctccc agttgtcttc
     2761 cacgaaccta atatcatgct cggcagggtc tatgccgatc gcggcgaggc tcccgaggta
     2821 caggtcctga acattgtcag ggctgggctt cagaatcacc tggtactgat ggtgttggaa
     2881 tagtcggtta gggttgtcgc cgtacctacc gtcggccggt cggcgagacg gctggacata
     2941 tgcaactgac catggctggg gcccgagaac cctgagaaag gtgtgtgggc tcatggtgcc
     3001 ggcgcctact tcgacgacat agggctgttg aatggtcaca tcgcgctgtg cccagtaacg
     3061 ttccatggtg agtatgaggt cctggaagtt caacattgtt ccctcccact gttagcgttg
     3121 gtgcggcagc gcccagagcg cagcatcaat atggtagtgc cgggtatccc ggggccatcg
     3181 cccggatgag agcgggagtc agcgaaagct ccacgactgc cgccagaatc agggcgggcg
     3241 cagcgaaaag aagcacaatc acaacaccct cggcaaaagc acgccccaga tcgcccgtgg
     3301 tcgcccgctt tctccacact agagggatga gaaaccctag ccgcatcccc actgcctgca
     3361 ccgcaataaa cgccggcagt tccagtatgc cgtggggtat gaaagacgca atgacgatgc
     3421 caagcggcca cccgccccgc tcgaccatga gcctagtgcc taccccgatg agcacaccgt
     3481 tcaggccaac actaagcata ggaacaatac ccaaaatcca tccaagcaga agcgagatca
     3541 gggacaccct taggttgttc caaaatagca ttagaatctg catgacgcgc gatttctgct
     3601 ccatggagtc tctgagggct gccaggttgc gaaacatcca cgggagttgg tcgccctgga
     3661 tctggtagtc cctgtatccc actaccacgc acgtagcaaa cagcacacag gcgaaaagga
     3721 aataccttcg gtacccccgg agggcccgtg gaagggcgcg agtatacagc acggatagcc
     3781 ttttcagcaa ccgaatgcct cctccacgaa aaaagccccc gcccctgagg atcctttggg
     3841 ggacgagagg ccatgttccc tgagcgcctg atactttcgg agcttttagt cttcactaac
     3901 tatagcaaac acaccggtac gtgtcaaacg tggctcaagc agcgctcagc agcgctccaa
     3961 tcttagtgtg cagcgcgacg caagggccgc tatggccccc accgccgcaa gctcaggggc
     4021 ccagatgact cccacgagac ctgccgccac cggcaagttg gctaacctgt cgccgtcgcg
     4081 ctcgatgatg agcctcatgg acgatccctg cctcaccgca tcggcgattc gctcggacaa
     4141 ccggctgtag gttgcggccc gagcttctgc tccagcggcg acccagcctt cctcatcagg
     4201 cccgccctcg ccgtcttgcg agcctaggtc acgttcggcc atgacgagtg cctgcacaag
     4261 atcgcctccg gcggcctcaa gatatgcggt agcctcctcg tacgtcagat cgactcgttc
     4321 gcgcagcctg tcgacggttg ccaggtctat cttcatcagc cctgcccgcc ctcctgctcc
     4381 atttttatca agtcgcggat gaagtcaagc gaatgcagct ttcgctctac ccgatgatcg
     4441 acatatgccc gaaagaccgt ttcaagctcg tcaagtgtat cctcccgtgg cttcagcaca
     4501 ccgacacgtt cataggtgag cgccagaagc agtctcgccg tttcccgggc ggccggtccg
     4561 aagctgaccg catcgcgcaa cgtgggccta caccgcccgc ataccagccc tcctgcctcg
     4621 ggcccaaacc acactgactg cccagtaggg gcgccgcaca gaacgcactg cccaagctcg
     4681 gggaggaagc ccgaggtggc catgaacttg atctcgaagg cgagcgacgc gatacgtggg
     4741 gccaccgctc ccgaatcgag cgcatcgaaa gctgcgacga ccaacgagta gagctggtcg
     4801 ttacgttcac gttcgccggt catcttatca acaagctccg ccatgtagga tgcgtaggcc
     4861 atccgggcga cgtcggacca gagactggaa tgggccgata ccagctcggc ggagctaatg
     4921 gtatcgaggt tcctgccacg aaagagcagc agattcacct gggcaaactg ctgcgttact
     4981 cctaccagcc tgcttctggg tcgccgcgct ccccgggcga gcgcttcgaa cttgccttcc
     5041 gagcctgaca gcactgtcag tatcctgtcc gcttctccga agtcctttac cttgaggact
     5101 attccctcac atctgaccag ggccattccg atcatcctta cctgcggacg cccgagtcgc
     5161 acaagtaacg tgcccccgag cgtgccgggc tattcagcgc cgggccggcg agtagcgttc
     5221 ctcttcactg aatatgcatc cacaatatgg ctggcggtac agccccatct ccctcgacat
     5281 ctgcacactc tcacggtatc cggtgcgaaa atcgtagtaa gcaaatggca cacccgccgc
     5341 ctcgcccgcc tccttcgcca cgcgcgtcag caattcatga ttctgatacg ggctgatcag
     5401 cagagtagtg gtgaaaccgt ccgctccgag ctcccgcgcc aacttcgccg cggggtacag
     5461 ccgcatgcgg taacactcgg cgcatcggcc gtctacattc ccatcgattt cgcctaggaa
     5521 ctggcggagc ccgtaggtct cgtctatgat ggcgctgtgc cccactgccg agcaatatgc
     5581 tttgaacgtg tcaagccgcc gcgcgaactc agtatatgga tgtatgttcg ggttgaagaa
     5641 gtagccactg acctcgtgcc cgtcagcgcg caaggacttg aggcagaacg tggtgcacgg
     5701 tgcgcagcag gaatgaagaa gcagtttcat agcagtgtcg cctgcccatc attgggaagc
     5761 tcgcggccta tgtgctcgta cgccctggcg
//
LOCUS       DAOTAC010000156         5786 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_11642_length_5786_cov_4.423312, whole genome shotgun sequence.
ACCESSION   DAOTAC010000156 DAOTAC010000000
VERSION     DAOTAC010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5786
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11642_length_5786_cov_4.423312"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3466)
                     /locus_tag="PLM74_07505"
     CDS             complement(<1..3466)
                     /locus_tag="PLM74_07505"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HON42956.1"
                     /translation="MASYTPEQEQAIFDLDRSLVVTAGAGSGKTRVLVGRYVNILQEQ
                     AADIDEVIAITFTEKAANEMKGRAWEEIVKREKGAGSPEDARRWRDLARQLQSARIST
                     IDSFCAQLVREYPIEAGVDPEFAIMDELDMRRAMFAAARSTVDKMLQRKDPYLTALVV
                     DKGFGVVAEALCQVFEKLRMTGKPFDECLGITMANLDRSEVAAERALADLRLVLLAAA
                     ESKGVPVNVDHLKLDRMQLGSPELFDVSGLVLKIDAVVAGLKLKKRSDEQKEIDALKA
                     VLHECLLDRFAREGVRSVVDACKLMDEEYRAQRGNGAMMDFTGLELAARDLLRDTPAG
                     AACRRRFKFVLVDEYQDTNRLQDEIVSLITGEPYGNRRFVVGDPKQSIYRFRGAVVEV
                     FEEAVRAASGDAAGLAAGGAGGCGRVELGTNFRSVGPLVDVTNALFTRILGPERFSPA
                     RAHRVAPDPDLARAELMLVHLDGKEKVPRGELEAEVVARRIADMVRNEERLVCETAAG
                     ADGPTTAARPVRYGDIAVLLRAMTHVKEFEAALTRHGIPYYVVGGTGFYGRPEVIWLV
                     DLLKAIDDGSDITSLAAVLRSPVFGLSDESLLRLKTAAGGLDRAVWGGEAELGTVDVA
                     GNVDVAVDWGLAGAELAKFRRARTSLRTLGNAVGRMPVGALVKTAVELARFEEYFAFV
                     EQGAQSIGNLRKLVQMAEDEASSRSCLRDFIADMEFAGDLSAREAEAAMEEESADTVK
                     VMTVHKSKGLEFPVVFVPELARPPRRSGGELFEYSLSLGIAAPVQVGDEWCNATRHFR
                     SVVEEIRAGDDDEGKRCLYVAATRASDYLVGSATLPGPGGRARSSGGPSWYEHLCSAI
                     PELDDQSPCGAGGSGGSDESGGVCSASVPATLVLDDSTGAAILVRHYEEWEIRDLMQA
                     MGVPVDEAAAGRESQTERVGTEAAGPGDESPGIAEFAPLAGPVRIAAGAANAPSQFSV
                     SALMCFSHCARWYLYEYVYRLGRIPRGLRHAFGAELRLNAAVRGQVVHSVCERCDRAT
                     DSAQARALLLDELHLRGADSVLAGSVADELMPMIDIFLTHYPGPMREGQKEIGFLMDV
                     GGPIVSGVMDRVDVLSDGSVLVVDFKTNRVLGPSVDEAAARYAVQLDAYAVAAGRVYN
                     ADRVTARLH"
     gene            complement(3459..>5786)
                     /locus_tag="PLM74_07510"
     CDS             complement(3459..>5786)
                     /locus_tag="PLM74_07510"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HON42957.1"
                     /translation="EFGVACAAPRKRLLSSSMLVLDCVALADAAASRHRGFDLARLLA
                     SPYIGRGPACAGQVARAIGVKGLELTSGRWKVRLQGADGCDDALTLADTLAEAWSGDF
                     GKSGGLADRVREFRALLAAVGAPESIMAADNRDWAIESWAAWRAFNSLLDEIARSVDG
                     EDGSGRDDGDEHGDGHRDDGGDWAEFARILGSAIRRRSFPLERPDPAGVKVVGPARSA
                     EGEWPVLFLSGMAEGTFPRRMRRPWVFKEDDLERLAHSGVGVTLPGEYAAMERFIFSS
                     AVGAASRKLYITYPSSAADGSSVRRSFFVDELLNSMDIAPEDERRITKSVSPREVFPR
                     RLDMAASPRELGLCALALAQTHGQAGQLMPQVREWAKQRPDMWEAASWWQDRSVQATG
                     PGFSVFSGAISAPDLLDSLEMKMLRGTAWSAGHFDDYLRCPFAFFCSRVLELGAVGEA
                     PEEPTPLTRGGVLHSILRRFFEAYLGSRLVPSGIDDYHSEMASIAQEELDKVDIEAIS
                     MSAPAWEAYRSSIVAQAIQTLDAQVELASKTDGFWSPAHLEWSFGLALRPEASPSSVR
                     DYLVLCGDDQGGCLGGDLGDGTPDTQGRRGSTMGGLRVCGRVDRIDVGPDGALAIYDY
                     KSGRSANLPSFTDIKEADEVQLGVYVLAVERLLASAPQLVAGAWYYSLRDNRMGSGVW
                     REEYHDRFAAERRRGGKLSSEKWSEYVGRVQASALDVAGKAAAGVFHIAPRKLSSCAW
                     CDYRRVCQVYMLDLEALSQSGDDDRDVCTGGDGRG"
BASE COUNT         1075 a         2102 c         1562 g         1047 t
ORIGIN      
        1 aatgcagcct ggcggtcaca cgatcggcgt tgtacactcg tcctgcggcg acggcatatg
       61 catccagctg caccgcgtac ctcgccgctg cttcatcgac tgacgggccc aggacgcggt
      121 tggttttgaa gtcgactacc aacacactcc cgtcagacag cacgtcgaca cgatccatga
      181 cccccgatac tatcggcccc cctacgtcca tcaggaatcc aatttctttc tgcccttccc
      241 gcatgggccc tggatagtgt gtcaagaaga tatcgatcat cggcatgagc tcatccgcaa
      301 ccgagcctgc aagaacactg tctgctccgc gcagatgcaa ctcatcgagt aataaggccc
      361 tggcctgggc agaatccgtc gcgcgatcgc acctttcaca taccgagtgt actacctgcc
      421 ctcgcacagc cgcattgagc cgcagttctg ctccgaaggc gtggcggagg ccgcggggta
      481 tgcgccccag tctgtacaca tactcataca gataccaacg ggcgcagtgt gagaagcaca
      541 tgagcgccga cacagaaaac tggcttggcg cgttcgccgc gccggcggcg atccgcaccg
      601 ggcctgccag aggggcgaac tcggcgatgc cgggcgattc atccccaggc ccggctgcct
      661 ccgtccctac tcgctcggtc tggctctccc gccctgccgc ggcctcgtcc accggcacgc
      721 ccatcgcctg cataaggtcg cgtatctccc actcttcgta gtgcctgacg agtatcgcag
      781 cgccggtgct gtcgtcaagt accaacgtgg ctggcacgct ggctgaacag accccgcccg
      841 actcatcgct cccgccggag ccccctgctc cgcatgggct ttggtcatcg agctcaggga
      901 ttgccgagca cagatgctca taccagctag gcccgcccga ggaccgcgct cgcccgcccg
      961 ggccgggcag tgttgcgctg ccgaccaagt agtcggacgc ccgcgtcgca gcaacgtaca
     1021 ggcagcgctt gccctcgtcg tcatcgccgg ccctgatctc ctccactacg gaccggaagt
     1081 ggcgagtggc attgcaccac tcatcgccca cctgcaccgg cgccgcgatc cccagcgaca
     1141 gactgtattc gaatagctcc ccacccgatc tgcgtggcgg tcgagccaat tcaggaacga
     1201 aaaccacggg gaactcaagc cccttggact tgtgaacagt catgaccttc actgtgtcgg
     1261 cggattcctc ttccatggcc gcctcggcct ctcgggcgct gagatcgccc gcaaactcca
     1321 tgtctgcaat gaaatcacga agacaggacc ggctcgacgc ttcgtcctcc gccatctgta
     1381 cgagcttacg gaggttgccg atggactgag cgccctgctc gacgaaggca aaatactcct
     1441 cgaacctggc cagttcaacg gcggttttca ccaaagcgcc caccggcatc ctgcccactg
     1501 cgttcccaag cgtgcgcagg ctcgtacgcg cccggcggaa cttagcaagt tcggcgcctg
     1561 cgagccccca atcgaccgcc acgtcgacat tgccagctac gtcgacagtg cccagctccg
     1621 cctcgccgcc ccacaccgcc ctgtccagcc cgcccgcagc cgtcttcaac cggagcagac
     1681 tctcgtcaga tagaccaaac acgggtgacc gcaggactgc ggcgagtgac gtgatgtccg
     1741 atccgtcatc gatggccttt agcagatcaa caagccatat gacctcgggc cttccgtaga
     1801 atccggttcc tcccaccacg tagtatggaa tcccatgtcg cgtcaaggcg gcctcgaatt
     1861 ccttcacgtg agtcatcgcc ctgagcaata ctgcgatatc cccatatcgc accgggcgcg
     1921 ccgctgtcgt cggaccatct gccccagcgg cggtctcgca tacaagcctc tcctcattcc
     1981 gcaccatgtc ggcgattcgc ctggctacaa cctctgcctc aagctcgccc ctggggacct
     2041 tctccttgcc gtccagatgc accagcatca attccgcccg ggcaaggtcg ggatccggcg
     2101 ccacccgatg cgccctggca ggtgagaacc gctccggccc cagaatcctc gtaaagagcg
     2161 cattcgtgac gtccacaaga ggcccgaccg agcgaaaatt ggtcccgagc tccacgcgtc
     2221 cacacccacc ggcgccgccc gcggcaagcc ccgcagcatc acccgaggcg gcccgcaccg
     2281 cctcctcgaa tacctccaca actgcgcctc ggaacctgta tatcgactgt ttcgggtcgc
     2341 ccacaacaaa ccgcctgtta ccgtagggtt cgcctgtaat cagcgagacg atttcgtcct
     2401 ggagcctgtt ggtgtcctgg tactcatcga cgaggacgaa cttgaacctt ctacggcacg
     2461 ccgccccggc cggggtgtcg cgcagcaggt ctctggcggc aagctctagg ccggtgaagt
     2521 ccatcatggc gccgttgccg cgctgagcgc gatactcttc atccatcaac ttgcatgcgt
     2581 ccaccacaga acgcacacct tcccgtgcga acctgtctag caagcactcg tgcaggactg
     2641 cctttagcgc gtctatctcc ttctgctcat ccgaccgctt cttcagtttg agcccggcca
     2701 cgaccgcatc gatcttgagc acaagaccgg acacgtcaaa gagctcaggg ctgcctagtt
     2761 gcatacgatc cagcttcaga tggtcgacgt tcaccggcac acccttgctt tcagccgcag
     2821 caagcagaac gagcctgagg tcggccagcg cccgctctgc cgcaacctcc gaccggtcca
     2881 ggttcgccat tgtgatgcca aggcactcat cgaacggctt gccggtcatg cgtagcttct
     2941 cgaagacctg gcagagagcc tctgccacaa cgccaaaccc cttgtccacc acgagagcag
     3001 tcaagtaagg gtccttacgc tgtagcatct tgtcaacggt cgagcgggcg gccgcgaaca
     3061 tagcccggcg catgtcgagt tcgtccataa tggcgaactc cggatccaca cctgcttcta
     3121 tggggtattc cctcacgagc tgcgcacaga aggagtcgat cgtgctgatg cgcgccgact
     3181 gaagttgcct cgcaagatcc ctccaccttc gggcgtcctc gggagaaccg gcacccttct
     3241 cccgcttgac aatctcctcc catgcccggc ccttcatttc gttcgcggcc ttctccgtga
     3301 aggttatggc tatcacttca tcgatgtcgg cagcctgctc ctgcaggatg ttcacgtatc
     3361 tccccaccaa aactcgggtc ttgcctgacc ccgccccggc tgtaaccacc aggctacggt
     3421 cgaggtcgaa tatggcctgc tcctgctcgg gagtatagct agccacggcc atcacctccc
     3481 gtgcacacat cgcgatcatc atcgccggat tggctcaagg cctcaaggtc aagcatgtac
     3541 acttggcaca cgcgtcggta gtcgcaccag gcacagctag acagcttgcg aggcgcgatg
     3601 tggaacacgc ccgcagcagc cttgcccgcc acgtccagtg cggacgcctg cactcggccc
     3661 acgtattcac tccatttctc gctcgaaagc ttgccgcccc ttcgccgctc cgccgcgaac
     3721 cggtcgtgat actcctcgcg ccataccccc gaccccatgc ggttatcgcg caagctgtag
     3781 taccaagcgc ccgccacgag ctgaggcgca gacgcgagca gtcgctccac ggccagcacg
     3841 tacactccaa gctgaacctc atcagcctcc tttatgtcgg tgaacgaggg caggttcgcg
     3901 ctgcgaccgg acttgtagtc gtatatagcc agggcgccat caggcccgac gtcgattctg
     3961 tccacccgac cgcacacgcg caggccgccc atggtgctgc cgcggcggcc ctgcgtgtcg
     4021 ggtgtcccgt cacccagatc gccgccaaga catccgccct gatcatcgcc gcataggacg
     4081 agatagtccc gaactgaact gggcgatgcc tccggcctga gcgcgaggcc gaagctccac
     4141 tcgaggtggg ccgggctcca gaacccgtcg gtcttgctcg ccagttccac ctgcgcgtca
     4201 agagtctgaa tggcctgcgc gactatcgag cttcgatatg cctcccacgc cggcgcggac
     4261 atgctgatgg cctcgatgtc gaccttgtcc agctcctcct gggcgatgct cgccatctca
     4321 gaatggtaat cgtctatccc ggacgggacg agacgcgacc ccaggtatgc ttcaaagaac
     4381 cgtcgcaaga tgctgtggag aacaccgcca cgcgtcagcg gggtcggctc ctccggcgcc
     4441 tcgccaaccg cgcctagctc cagcacacgc gagcagaaga atgcgaacgg gcatctcagg
     4501 tagtcgtcga agtgcccggc cgaccatgcc gtgccgcgaa gcatcttcat ctccaggctg
     4561 tccagcaggt caggcgcgct gatcgccccg ctgaacaccg aaaaccctgg gcccgttgcc
     4621 tggacggacc tgtcctgcca ccacgacgca gcctcccaca tgtccggccg ttgtttcgcc
     4681 cattcccgaa cctggggcat cagctgaccc gcctgcccat gcgtctgggc gagcgcaagt
     4741 gcgcacagcc cgagctccct ggggcttgcc gccatgtcca acctgcgcgg gaacacttca
     4801 cgcggagata ctgacttggt tatccgccgc tcatcctccg gcgcaatgtc cattgagttc
     4861 aggagctcat ctacgaagaa ggatcgcctc accgacgagc cgtcggcagc gctggacggg
     4921 tatgttatgt acagcttcct gcttgccgcg cccactgccg agctgaagat gaaccgctcc
     4981 atggctgcgt actcgcctgg caatgtgacg cccacgcccg agtgagctag ccgctccaag
     5041 tcatcctcct tgaagaccca cggtcgcctc attctccgtg ggaacgtgcc ctccgccatc
     5101 ccactcagga atagaaccgg ccattccccc tccgcgcttc ttgccggccc gacaaccttg
     5161 acaccggcag ggtccgggcg ttccagcgga aacgagcgcc gcctgatcgc agagccgagg
     5221 atgcgggcga attcagccca atcgccgccg tcgtctctat ggccatctcc atgttcgtct
     5281 ccatcgtcgc ggcccgaccc gtcctccccg tcaactgagc gggcaatctc gtccaggagc
     5341 gagttgaacg ccctccatgc cgcccacgat tcgatggccc aatcacggtt gtcggctgcc
     5401 atgatggact ccggcgcccc gaccgccgcg agcagcgcac ggaactcgcg cacacgatcg
     5461 gcaagccctc ccgatttgcc gaagtctccg ctccatgcct ccgccagcgt gtcggcgagc
     5521 gtaagcgcat cgtcgcatcc gtcagccccc tgcagacgta ccttccaccg cccggacgta
     5581 agctccagtc ccttgacccc gattgcccgg gcgacctggc cggcgcatgc cggcccacgg
     5641 cctatgtatg gactggcaag caacctcgcg aggtcaaatc ctctgtggcg cgacgctgcg
     5701 gcatctgcca gagccacgca gtcgaggacc agcatcgacg atgacagcag acgtttcctc
     5761 ggcgccgcgc acgccactcc gaactc
//
LOCUS       DAOTAC010000157         5743 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_11740_length_5743_cov_4.404536, whole genome shotgun sequence.
ACCESSION   DAOTAC010000157 DAOTAC010000000
VERSION     DAOTAC010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5743
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11740_length_5743_cov_4.404536"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..411)
                     /locus_tag="PLM74_07515"
     CDS             complement(94..411)
                     /locus_tag="PLM74_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HON42958.1"
                     /translation="MDGARRRSDVVISDEAMQSLLLHARFLAQVGEAAARRLVEEFDE
                     KAKSLEEYPEHNPWLSDSLMPAGKYRKLLMGKTYLLVYQVKGSTVYVDAVVDCRKDYS
                     WLL"
     gene            complement(395..682)
                     /locus_tag="PLM74_07520"
     CDS             complement(395..682)
                     /locus_tag="PLM74_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HON42959.1"
                     /translation="MINIKPSAAIRRDYNSISELCKRSGEPVYLTKNGEGDLVVMDIE
                     AFARRESMLRLRESLVAAEEGRLAGKSGYSLDDVSSMMKAAIQEVLDGRRA"
     gene            complement(866..2032)
                     /locus_tag="PLM74_07525"
     CDS             complement(866..2032)
                     /locus_tag="PLM74_07525"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HON42960.1"
                     /translation="MQVFRAYFKVMRASAGAISIYVAIFLGLSVAFSLAEAPQETGDF
                     AQTRTSIAVINRDGEAPLAQGLADYLADTSGIVPYPDNPEKLQDALFFRNVEYIAIIP
                     AGFSTDFLSGRDCAVQKVTVSGSISSRYVDMRIDKYLRTARLRHQYGGDQSHEELAAA
                     IRADLAQETPVTVLPSSATNGSEREFVFYYRYFAYAVLATIMMGVSAIMMAFNKPDLR
                     RRSLCTPIPARSANLQLAAGHTIFALGCWSVLVLFSFVLYGRNLLSSGLVWLYCMNTL
                     AFTVVSASIGFLVGSSVRSNGAQAGAVNVIANGMSFVCGVFVPQSILSKHVLDFGRFL
                     PAYWYVKANDAISGLSRPTASALRPIYGDVLIQLAFAAAIFAVTLLLTKERQAE"
     gene            complement(2019..3161)
                     /locus_tag="PLM74_07530"
     CDS             complement(2019..3161)
                     /locus_tag="PLM74_07530"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HON42961.1"
                     /translation="MFMHIFGYRLKCLARDREMVFWTLIFPLILATMFHFAFDQLMGR
                     QETFRPIRAAVVGSDAYRQNVAFRQALDALSRPGEGQLIELTVTDDREARRLLEDGAV
                     SGVITVGDPIGLTVSQSGIEQSILKAILDEYAHTYATITGILEEHPTDPAGLLREIGQ
                     RRSYTQQISFSSAEPDTILNVFYVLIAMTCLYGGFLGLRNTTDVQADLSPQGARRSVA
                     PTHKLGVVLYDLAAALTISLAEVTVLLAYLVLVLRVNFGNQVWYVLLTCLAGCIAGVS
                     FGAFIGTFARKSEGVKTAILSASSMTMSFLAGLMIVNMKDIIARKAPFLSYINPAALI
                     ADAFYSLYVFASHRRFFLNIGLLLLISAAMCTLSFLRLRRDRYASL"
     gene            complement(3179..4111)
                     /locus_tag="PLM74_07535"
     CDS             complement(3179..4111)
                     /locus_tag="PLM74_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006568959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON42962.1"
                     /translation="MLVRVQNLVKRYGDVLALDHFNLEVGEGEILGLLGPNGSGKTTT
                     INCILSLLKYDKGAIEVFGKPMAPDAYESKRNIGVVMQNVAVFQELRVEENIDYFCGM
                     YVNDAKARRRLVDEAIEFVGLGEFRRFYPKKLSGGLLRRLNIACGIAHKPKLIFMDEP
                     TVAVDPQSRNKILEGIRELNRQGATVVYTSHYMEEVEQLCTRIVIMDKGKTLATGTKD
                     ELKAMINDGETVTVELYALQPDHVANIRQLPHVARAEYADNRLAVHFDGGQHNLLTLL
                     DYLKSRDLSFGRVFSEQPTLNDVFLEITGRQLRD"
     gene            4334..5428
                     /locus_tag="PLM74_07540"
     CDS             4334..5428
                     /locus_tag="PLM74_07540"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HON42963.1"
                     /translation="MNAIVDRVLIWACCAALLPQRAGDAMIVIAMLAAITVSGLNGYL
                     LSPTFHLASIVAYAGASAVWPSFCLFIPLVYYDAFGLKQAWWAPAVAAALAACFTGLP
                     ANTSLTVAVLMAAAYVLKHRAVALERSRTELKQLRDSGQELAMMLSQKNKDLIEKQDY
                     EVRLAMLGERSRIAREIHDHVGHLLSRSILQVGAIMAAKRSADTAAADADLRQSLSVV
                     RDTLSQAMNTIRASVHDLHEESVDLRTQIDALAQGFEFCPIHLDYRLDSQPEKEITYC
                     FIAIVSEGLNNVIRHSNATQVTLRLIEHPALYQLIVEDNGSKTAGEPSGGLGLRSMAD
                     RVAALGGAALHRTPGRVQTVCLDSERRASA"
     gene            5425..>5743
                     /locus_tag="PLM74_07545"
     CDS             5425..>5743
                     /locus_tag="PLM74_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008688326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HON42964.1"
                     /translation="MRVVIIDDDRLVCQSLKTILQSDEDIEVVSMGHDGHEAVALFTQ
                     LQPDVLLMDIRMDRMTGLEACRRILREFPDARILFLTTFLDDEYIVQALRLGAKGYIL
                     KQDF"
BASE COUNT         1223 a         1724 c         1596 g         1200 t
ORIGIN      
        1 tccagccgtt ttgtctgacg gccgtgatgt caagaccagc taaacggcta ttccagcccg
       61 gcgaggcgct caatcgtgat tcgtagaggc cggctacagc aaccagctgt aatccttcct
      121 gcaatccacg acggcgtcca catacacagt gctgcctttt acttggtaga ctagcagata
      181 ggtcttgccc atcaacagct tgcggtactt gcccgccggc atcagtgaat ctgagagcca
      241 tgggttgtgc tccgggtact cctcaagaga cttcgccttc tcgtcgaact cctctacaag
      301 ccgacgcgca gcggcttcgc cgacctgtgc cagaaagcgg gcgtgtagca gcagactctg
      361 catcgcctcg tctgagatca ccacatcgga tctcctacgc gcgccgtcca tccaatacct
      421 cctggattgc ggccttcatc atggaggata cgtcatcgag agaataaccg ctcttcccgg
      481 ctaatctgcc ttcctcggca gcgaccaggc tctccctgag acgcaacatg ctttcgcgcc
      541 tcgcgaatgc ttcgatgtcc atcaccacca ggtcgccttc gccgttcttg gtcaggtaca
      601 caggctctcc cgaacgcttg cagagctcgg atatgctgtt gtagtccctc cgaatcgccg
      661 ccgacggctt gatgttgatc atctggacca cctcgtctgg tagtatcata gcatgcttat
      721 tatatgctta ttatatcatt ctgggatcgc aacgggttcg accccatctc ggcagctgcc
      781 cgggccggac tgcgcgccgc atcgctcacg gccagcacgg ccctcactgc cctcacggcc
      841 cccaagaccc gcgtacccgc tccgcctact ccgcctgccg ctctttggtg agcagcagag
      901 ttaccgcgaa gatggcggcg gcaaacgcca gctgaatcag tacatcgccg tatatcggcc
      961 gcagggcgct ggcagtaggc cgggaaagcc ccgaaatcgc gtcgttagcc ttcacatacc
     1021 agtaggcagg taagaaccgt ccgaaatcca ggacgtgctt gctcaggatc gactgcggca
     1081 caaacacccc gcagacgaaa ctcataccat tggcgatcac attcaccgcc ccggcctgtg
     1141 cgccgttgct ccttaccgag ctgccgacga ggaatcctat gctggcgctc acgacggtga
     1201 atgccagcgt gttcatgcag tacagccaca ctagcccgga tgacagcagg ttcctgccgt
     1261 acagaacgaa actgaaaagc accagcaccg accagcagcc cagcgcgaat atggtgtggc
     1321 cggcggccag ttgcaggttc gcactgcgcg ccggaatcgg agtgcacagg ctgcgccgac
     1381 ggaggtcggg cttgttgaat gccatcatga ttgcgctcac acccatcatg atggtagcga
     1441 gcacagcata tgcaaaatac cggtagtaga agacgaattc tcgctcagag ccgttggttg
     1501 ctgaagaagg caaaacggtg accggggtct cctgcgccag atcggcccgg atagcggccg
     1561 ccagttcttc gtgtgactga tcccccccat actgatggcg gaggcgagca gttctgaggt
     1621 acttgtcgat ccgcatatcc acgtaacggc tgcttatgga acccgatacc gtcactttct
     1681 gtacggcgca gtcgcgaccg gacaggaagt cggtagagaa accggccggg ataatcgcaa
     1741 tgtactccac attgcggaag aacagcgcat cctggagctt ttccggattg tccggatagg
     1801 gcactatccc actagtgtcg gcaaggtagt ctgccagccc ctgcgcaagc ggggcttctc
     1861 cgtcgcggtt gatcacggcg atggacgtcc tcgtctgggc gaagtcccca gtttcctgcg
     1921 gagcctcggc gagggagaac gccaccgaca gccccaggaa gatggcgacg tagatggata
     1981 ttgcccccgc tgaggcacgc atcactttga agtaagccct aaagacttgc atatctatcc
     2041 ctcctcaggc gcaggaaact caaggtgcac atggccgccg aaatcaggag caacagcccg
     2101 atgttgagga agaaccggcg gtggctcgcg aaaacgtaca ggctgtagaa cgcgtcggcg
     2161 atcagggccg ccgggttgat gtaggacaga aacggcgctt tgcgtgcgat tatgtccttc
     2221 atgttgacta tcatcaagcc cgccaggaag ctcatcgtca tgctcgacgc gctgaggatc
     2281 gccgtcttta cgccctcact ctttcgggcg aacgtaccga tgaaggctcc gaaagacaca
     2341 ccggcaatgc agcctgccag acatgtcaac agcacatacc atacctggtt tccgaaattc
     2401 acgcgcagca caagcactaa atacgccagc aggaccgtca cctctgccag gcttatggtg
     2461 agcgctgccg ctaagtcgta cagcactacg ccgagtttgt gagtgggcgc cacactgcgc
     2521 cgcgcgccct gaggggacaa gtccgcctgc acgtcggtgg tattgcgcag ccccaggaaa
     2581 ccgccgtaaa gacaggtcat cgcaatgaga acatagaaga cgttgagtat ggtgtcaggc
     2641 tcagcgctgc tgaaagagat ctgctgtgtg tagcttcgcc tctgccctat ctcccgaagg
     2701 agtcccgccg gatctgtcgg atgctcctcg aggataccag tgattgttgc gtatgtgtgt
     2761 gcgtactcat cgaggatcgc tttcaatatg ctctgctcaa tgccggattg actgactgtg
     2821 agtccgatgg gatcgccgac ggtgatgacg ccagatacgg ccccgtcctc gagcaatcgg
     2881 cgagcctctc ggtcatcggt cacggttagc tcaatcagct ggccctctcc tggcctggac
     2941 agcgcgtcta gggcctgtcg aaaggcgaca ttctgtcgat aggcatcgct cccgactacc
     3001 gcggctctga tgggtctgaa agtctcctgc ctccccataa gctggtcaaa ggcgaaatgg
     3061 aacatggtgg ccaggatcag cgggaagatc aaggtccaga ataccatctc tctgtctcgc
     3121 gccagacact tcagacgata gccgaatatg tgcatgaaca tgccttccca cactccccct
     3181 aatccctcag ctgtctgccg gtgatctcaa ggaacacatc gttcagtgtc ggctgttccg
     3241 aaaagacccg gccgaacgag agatcgcgcg atttcaggta gtcgagcaga gtcagcagat
     3301 tgtgctggcc tccgtcgaaa tggaccgcca gacggttgtc cgcatactcc gcgcgggcga
     3361 catgaggcaa ttgccgtata ttggctacgt gatcgggctg gagagcgtac agctcgacgg
     3421 tgacggtctc gccgtcattg atcatcgcct taagctcgtc cttcgtgccc gtggccagag
     3481 tcttgccctt atccatgatc acaatgcggg tgcagagctg ttccacctct tccatgtagt
     3541 gcgaggtgta caccacagtc gcgccctgcc ggttcagctc gcggatgcct tccagtatct
     3601 tgttcctact ctggggatcc acagctaccg taggctcatc catgaaaatc agcttgggtt
     3661 tgtgggcaat gccgcaagcg atattgagcc ggcgcaagag cccgcccgac aacttcttgg
     3721 ggtagaacct gcggaattcc cccaggccta cgaattcgat ggcttcatcc accagccgac
     3781 ggcgagcctt tgcgtcattc acatacatgc cacagaaata gtcgatgttc tcctctaccc
     3841 taagctcctg gaagaccgct acattctgca tcacgacgcc gatattgcgc ttgctttcgt
     3901 aggcgtcagg cgccatcggc ttgccgaaca cctcaatagc acccttgtcg tatttcagga
     3961 gcgacagaat gcagttgatt gtagtggtct tgcccgatcc gtttggaccc aagaggccaa
     4021 ggatctcgcc ttcacccact tctaggttga agtgatccag ggccagcaca tctccatatc
     4081 gctttacaag gttctgcacc ctaacgagca ttacgattcc tcccgatctc gttgtgcttt
     4141 cattctacct gcggcgccaa ggcgccgtaa gtgaatccag tcagttctgt ggatgacagt
     4201 tgtcactatc attgtcacga tgcgactgcc catcatgcga ctgcctatca tgcgactgcc
     4261 gaccgtcagt gtcagtcgaa aattgtcagt cggaaccagg cgtgatagac ttgatcccga
     4321 aggagacgta gctatgaacg cgattgtcga tagggtcctc atatgggcgt gctgcgccgc
     4381 cctgctgccg caaagggcgg gcgacgcgat gatcgtcata gcgatgctgg ctgcaataac
     4441 agtcagcggg cttaacggtt atctgttatc gcccacgttc cacttagcca gcattgtggc
     4501 ctacgccggg gcaagcgcgg tctggcccag cttctgcctc tttatccctc tggtatacta
     4561 tgatgcgttc gggctcaaac aggcctggtg ggcgccggcc gtagcggcgg ctctggcggc
     4621 atgcttcacg ggtcttcctg cgaacacatc actgactgtg gcagtattga tggcggcggc
     4681 ctatgtgctg aagcatcgcg cggttgcgct ggaacggagc cgcaccgagc tcaagcagct
     4741 gcgcgatagc ggtcaggaac tggccatgat gctcagccag aagaacaaag acctaataga
     4801 gaagcaggac tatgaggtta gattggcgat gctcggagaa cgtagtcgca tcgcccgcga
     4861 gatccacgac cacgtagggc acctgctatc gcggtcgata cttcaggtcg gggcgatcat
     4921 ggcggccaag cgcagcgccg acactgcggc agcagacgcc gatctgcgac agagcctatc
     4981 ggtggttcga gatactctat cgcaggccat gaataccatc cgggccagcg tccacgattt
     5041 gcatgaagaa tccgttgact tgaggaccca gatcgatgct ttggcccagg gattcgaatt
     5101 ctgtccgatt catctcgact acaggctgga cagccaacca gagaaggaga tcacatactg
     5161 cttcatcgcc atcgtctcag aagggcttaa caacgtcatc cgtcattcca acgccaccca
     5221 ggtcaccctc aggctcatag aacatcccgc gctttatcag ctcatcgtgg aggataacgg
     5281 atccaagacc gcaggcgaac cctcaggcgg tctcggcctg cgcagtatgg ccgaccgcgt
     5341 cgccgcgctg gggggggcag ctcttcatcg aacaccaggg cgggttcaga ctgtttgtct
     5401 cgattccgaa aggagggcgt ctgcatgaga gtagtgatca tcgacgatga tcggctggta
     5461 tgtcagtctc tgaagaccat cctgcaatca gacgaagata tcgaggtcgt ctcaatgggc
     5521 catgacggcc atgaagcagt ggcgctcttc acgcagcttc agccggacgt gctgctgatg
     5581 gacatccgaa tggaccgcat gaccgggctc gaggcatgca ggcgcatcct gagagagttc
     5641 ccagatgccc ggatactctt ccttaccacg tttctggacg acgagtacat cgtgcaggcg
     5701 ctccgcttgg gcgcgaaggg ctacattctt aagcaggact ttg
//
LOCUS       DAOTAC010000158         5742 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_11745_length_5742_cov_4.818885, whole genome shotgun sequence.
ACCESSION   DAOTAC010000158 DAOTAC010000000
VERSION     DAOTAC010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5742
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11745_length_5742_cov_4.818885"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="PLM74_07550"
     CDS             complement(<1..667)
                     /locus_tag="PLM74_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HON42965.1"
                     /translation="MMFENLSARLQGIFKNLRNRGKLTEKEVDLALREVRTALLEADV
                     NFKVAKAFIDRVRKRAVGEEVLTSLTPAQQVIKIVHEELTDLMGGQSSKIVFSSKPPT
                     VLMLVGLQGSGKTTSCAKLASLMRKQGRKPLLAAADVYRPAAIKQLQVLGAQLGVEVY
                     SLGERADPADIAAGAVERARSMGNDTVILDTAGRLHIDDEMMEELERVKARVAPTEIL
                     LVVD"
     gene            complement(664..1182)
                     /locus_tag="PLM74_07555"
     CDS             complement(664..1182)
                     /locus_tag="PLM74_07555"
                     /inference="COORDINATES: protein motif:HMM:NF016205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxM family DNA-binding protein"
                     /protein_id="HON42966.1"
                     /translation="MLRKMLRMAALFDVYGPLLTERQQEMCRLYYLNDYSLSEISELH
                     VVSRQAVHDTLVRSEQAMEEYENQFGLVRSSDTRRLQLEELTSALSAAREALDSAVRA
                     LGPEIRGNCECDLDAVRAWLARAIEAVEGCQKAAISMDADTGTGTVTDTGTGMGTDLG
                     GDHAIGEKEGGL"
     gene            complement(2127..3050)
                     /gene="ftsY"
                     /locus_tag="PLM74_07560"
     CDS             complement(2127..3050)
                     /gene="ftsY"
                     /locus_tag="PLM74_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HON42967.1"
                     /translation="MSPSLFSAIRNGLKKTRDAFAGKIESIFEGRPALDEETLDELEA
                     VLIQADLGVTAATSIIDSVRSRAARGERLDAEGAMDLIEKELLGTLGSEAIPLTQADA
                     PPTVIMVVGVNGTGKTTSIGKIAAMLVSMKKRVILAAADTFRAAAIDQLEVWGRRTGC
                     AVVRHQEAADPAAVAFDAYQAARARGADYLIVDTAGRLHTKSNLMEELKKIKRVIARE
                     CPSAPHEVLLVVDATTGQNAIAQARVFNEAVGVTGIVLTKLDGTARGGIVVAITQELG
                     IPVKFVGVGESVEDLRPFDPDEFVHGILRRD"
     gene            complement(3069..>5742)
                     /gene="smc"
                     /locus_tag="PLM74_07565"
     CDS             complement(3069..>5742)
                     /gene="smc"
                     /locus_tag="PLM74_07565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HON42968.1"
                     /translation="AERDAGRLNVAAQRHQALVERGSQLAAMVAERRERQIKLTDDYE
                     TACLNARQARAEYVERGSAARQTEERMAALTAARAAAEAEVEECKSALFDVLAHASDL
                     RSRVRAGEDSMKYNRSRAVRIQAQLDEKDREYARLQHEVERIMREGRALIDQSVEADS
                     RVSGARTALEAARAEQELAAERAARARADESSAQAAYVSLSALQRDYEGYGRAVRALL
                     TSDEWRRAGLLGAVGELVSAPREYERAIEAALGPAVQNIIASTSGVAEKAISFLKESH
                     AGRATFLPLDILRPSTVPSSQIPKTAPGVIGLASDLAESAPQHRKAVDYLLGRVLVVQ
                     DLRCGVALIRSGVRLRMVTLDGDQISAGGAMTGGEATDRQGGLLARGRRLEELQEQLE
                     RARLESAKAESDRARAHARVAQCQADLEEAERELAGLRLAVQGQNERLKLAQEALPRS
                     ADELASLELELKSVAAEDERTSAEVKEYTRQLEVVESDRIQLEAELESKSGALTQLKD
                     EEAQSAADHSTLNADTAALRERVSALEVERSRAQAEVESCHAELERLNDQERAAAEEA
                     ALALQEVERLREAASSSAAAFENEQKQLEAARARRADELVLVNEAERASRSARRGTSA
                     ADSRLSDARVGEARLDAERAAIAERLFASYSVGAEEALSRDVSALSLSPEEARLEIKR
                     LRQEIDDLGPVNHTSIEESKELAERHHFLKEQLADMESAQESLSEVIRECDRVCIKQF
                     TETFEAIRGEFSDIFQDVFGGGAADLALDDPSAPLECGVEIVCQPPGKRLTSLSLLSG
                     GEKALAAIALLFAIMRVKPSPVCVLDEIDSALDEANVARFVELLEGISRKVQVIIVTH
                     RKRTMECADTLFGVTMEESGVSKVFSIRATDYKL"
BASE COUNT         1051 a         1909 c         1561 g         1221 t
ORIGIN      
        1 cgtccacgac cagtagtatc tcggtgggcg cgacccttgc ctttaccctc tctagctcct
       61 ccatcatctc gtcatctatg tgcaaacgtc ctgctgtgtc caatatgacg gtgtcattcc
      121 ccatgctgcg cgcacgctct accgcaccgg cggcgatgtc ggccggatcg gcgcgctcgc
      181 ctaaggagta cacctccacg cccaactgcg ctccgagcac ctgcagttgc ttgatcgcag
      241 ccggcctgta gacgtctgcg gcggccaaaa gtggttttct cccctgctta cgcatgaggc
      301 tcgcaagctt cgcacagctg gtagtcttgc ccgagccctg cagcccgact agcatgagga
      361 ctgtgggagg ctttgaggaa aagactatct tcgatgactg cccacccatg aggtcggtga
      421 gctcctcatg gacaatcttt atcacctgtt gtgcgggcgt gaggctcgtt aagacctctt
      481 cgcccacagc acgcttcctg accctgtcta tgaatgcctt agccaccttg aagttgacgt
      541 ccgcttccag cagagccgta cggacctcac gaagtgccaa gtctacttct ttttcagtga
      601 gcttcccgcg gttacgcagg ttcttgaaga tgccctgaag ccgcgctgag aggttctcaa
      661 acatcaaagc ccaccttcct tctcgccgat cgcatggtcg ccgcccaggt ccgttcccat
      721 gcccgtgccc gtgtcagtga cagtgccggt gcccgtgtcg gcgtccatgg agattgcagc
      781 tttctgacag ccctcaaccg cctctatggc tcttgcgagc caggcgcgaa ccgcatccag
      841 atcgcactcg caattgcctc taatctcggg cccgagagca cgcactgcgc tgtcgagggc
      901 ctcgcgcgcg gctgaaagag ctgatgttaa ctcttcgagt tgcagtcgcc tggtatcaga
      961 agatcggaca agcccaaact ggttctcata ctcctccatc gcctgctctg accggacaag
     1021 cgtgtcgtga acggcttgcc gtgacactac atgaagctcg gatatctccg acaaggagta
     1081 gtcattcagg tagtacagac ggcacatctc ctgttggcgc tcagtcaaaa gcggcccata
     1141 cacatcgaac agggcagcca tcctcaacat ctttctaagc atcgaccacc gaccaccccg
     1201 caggtgttaa ggccaaggcc tttacactac gcagtatagc ggccatggcc ccatgtgtca
     1261 aggcaccgac ttgctgcgag aagctgacat ggaggagtac ggtcccaaac agacacctct
     1321 gcagttcatc tggcaagggc agaatgccct cgcagatccc atgagtttgc cggtttgccc
     1381 catcgcccgt aggttcttcg caaggtcctt ggcggaacct gtcggaaaac cccgcggttc
     1441 ctataggcta gataaagcca gcagacctgc agcaggacgc acggttttcg cgacaaccta
     1501 tgcagaggga gcaagatccg gtcatggcga cggcgcgcga ggacgttgcc aattacaggt
     1561 atttgcgtac atcggggcgt tttccggaga ttttctgcag cctgcgtgcg aaaaatctcc
     1621 gcgaacggtc ccaaattcgc tgagaacccc attgcccatt atgaaactgc cagagccgaa
     1681 cgggactagc acgcagaatt gggccaatca ggcaatatca ggggactgaa cccaaagtct
     1741 cccggatata tccttatata tacagccgtc attgcagacg aggtgactct cggtcgcgag
     1801 agtcggcttg aaaggacatt tttcgccacg atgcggcgaa aaacttcaat cgttggacct
     1861 ctgccatgca aaaacctgta attgcctccc ccgcgatatg ataagagctc ggtctctggt
     1921 gtcggactac actctcactg ctcccaggtc gcccctgacc ctaaccctaa tcgcccgcac
     1981 gacgagcgtc tacgatggcc gccagccttc ccccgggccg cgggaccgag gttgaaggtt
     2041 tcggccagcg cgtcctagct ggggttttcg caatcgaggg tttcgccgcg cacgacgacc
     2101 gctgaccgcg aaggtcacgg attgtgtcag tctctgcgca aaatcccgtg cacgaactca
     2161 tcagggtcga acggtctgag atcctcgacg ctttccccca cgcctacgaa cttcacaggg
     2221 atacccagct cctgagtgat cgcgacaact atcccgcccc gtgcggtacc gtccagcttg
     2281 gtcaagacta tcccggtgac tccgacggcc tcgttgaaaa ccctagcttg agcaatggca
     2341 ttctgaccag tggtggcgtc tactaccagc aacacctcat gaggcgccga cgggcactct
     2401 cttgcgatga ctcgcttgat cttcttcagc tcctccatga ggttgctctt ggtgtggagc
     2461 ctgccggcgg tatccacgat gagatagtcg gcgccgcgcg cacgggctgc ctgataggca
     2521 tcgaaggcaa ccgcggccgg gtcagctgcc tcctgatggc ggactactgc acatccggtt
     2581 cgtcgccccc acacctccag ctggtcaatg gctgcagcgc ggaatgtatc ggcagctgcc
     2641 agaatgactc gcttcttcat cgacaccagc atcgccgcga tcttgccgat ggacgtggtc
     2701 ttgcctgttc cgtttacccc cactaccata atcacagtgg gcggggcatc ggcctgcgtc
     2761 agcggaatcg cttccgaacc caacgtacca aggagttcct tctcgatgag gtccattgcg
     2821 ccctctgcgt ccagcctctc tccacgggct gcgcgggacc gcacagagtc gatgatcgat
     2881 gttgcagcgg tgacgccaag gtcagcctgg ataaggacgg cttccagctc atccagggtt
     2941 tcctcatcca gagccggcct gccttcgaag attgactcta tcttgccggc gaaagcatct
     3001 ctggtcttct tcagtccgtt gcggattgct gagaaaagtg aaggcgacat cggaataccc
     3061 ccatcatgtc aaagcttgta gtcggtcgct cttatggaga acaccttgga tacgccggac
     3121 tcctccatgg ttaccccaaa tagggtgtcg gcgcattcca tggttcgctt tctgtgtgtg
     3181 acgattatga cctgtacttt cctggagatg ccttcgagca gttcgacaaa acgggcgacg
     3241 ttggcctcat ccagtgcgga gtctatctcg tctagaacgc acactgggct tggcttcacc
     3301 ctcatgatcg caaacagaag tgcgatggcc gccaaggcct tctctccacc cgacagcagc
     3361 gacaggctcg tcagcctctt ccccggcggt tggcacacga tttccactcc gcattccagc
     3421 ggagcgctgg ggtcatccag cgccaggtcg gcggcgccgc cgccaaacac gtcctggaag
     3481 atatcggaga actcccccct gatggcctca aaggtctccg tgaactgctt gatacacacg
     3541 cggtcgcact cgcgaatcac ttcgcttagg gattcctggg ccgattccat gtcggcgagc
     3601 tgctccttta ggaaatgatg cctctctgcc agctccttgc tctcctctat ggatgtatgg
     3661 ttgaccgggc cgaggtcgtc tatctcctgg cgcaggcgct tgatctccag acgcgcctcc
     3721 tccggcgaca gtgacagggc cgacacgtct ctcgagagag cctcctcagc gccgactgag
     3781 taggatgcga aaagcctttc ggcgatggcc gcacgttccg catccaggcg ggcctcgcca
     3841 actctggcgt cgctcagccg actgtcggcg gcagacgtcc cacggcgcgc gctgcggctg
     3901 gctcgttccg cctcgttcac caacacgagc tcgtcggcgc gccgtgcccg agcggcctcc
     3961 agttgcttct gttcgttttc gaaagcggct gctgacgatg acgccgcctc acgtagcctc
     4021 tccacctctt gcagcgccag tgccgcctcc tctgcagccg ctcgctcctg atcattcagg
     4081 cgctcgagtt cggcgtggca cgactccacc tcagcctgag cccttgacct ttccacttcc
     4141 agtgcgctca ctctctcgcg aagcgccgcc gtatctgcgt tcagagttga atgatcggcc
     4201 gccgactgtg cctcttcgtc cttgagctgc gtgagggctc cggacttcga ctcaagttcg
     4261 gcttccagct gaatccgatc tgactctacc acttcgagct gacgcgtata ctccttaacc
     4321 tcggccgatg ttctctcatc ctcggccgca accgatttca gctccagttc caagctcgcc
     4381 agttcgtcag ccgaccgcgg cagcgcctcc tgcgcaagct tgagtctctc gttctgccct
     4441 tgcactgcca accgcaaacc cgccagttct cgctctgcct cttccaaatc ggcctgacac
     4501 tgagccaccc tagcgtgcgc ccgcgcacgg tccgattctg ccttcgccga ctccagcctt
     4561 gcgcgttcca gttgctcttg cagctcttca agccttctgc cacgtgccag gaggccgccc
     4621 tgcctgtcgg tggcctctcc cccagtcatg gctccgccgg ccgatatctg atcaccatct
     4681 agggtgacca tgcgaaggcg cacgcccgat ctgatcaggg caacaccgca cctgagatcc
     4741 tgcacgacca ggacgcggcc gaggaggtag tccacagcct tgcggtgctg cggcgctgat
     4801 tctgcgaggt ctgaggcgag cccgatgacg ccgggcgccg ttttcggaat ctgcgagctg
     4861 ggcactgtcg acggacgcag gatgtcgagc ggcaggaacg tagcgcgccc ggcatggctc
     4921 tccttaagaa acgagattgc cttctctgcc acacctgatg ttgaagcgat gatattctga
     4981 accgccggcc ccagggctgc ctcaatggct cgttcgtatt cgcgcggtgc gctgacgagt
     5041 tcgcccacgg cccccagaag ccctgcgcgt cgccactcat cgctggtcag aagggcccgc
     5101 actgcgcgtc catacccttc gtagtcgcgc tgaagcgccg acaggctaac gtacgcggct
     5161 tgggcagagg actcatccgc ccgtgcgcgc gccgcccgct ctgcagcgag ttcctgttca
     5221 gcgcgggccg cctccagtgc ggtccgtgcc ccggataccc tggaatcggc ttccacagat
     5281 tggtcgatga gcgcccgccc ttcgcgcatg atgcgctcta cctcgtgctg aaggcgcgca
     5341 tactcccggt ccttctcgtc aagctgtgcc tgtattcgca cggccctgga tcggttgtac
     5401 ttcatggagt cctcgcctgc gcgcactcta gaacggaggt cagaggcgtg cgctaagacg
     5461 tcaaagagcg cagacttgca ctcttccact tctgcctcgg cggcggccct ggcagcagtc
     5521 aacgcggcca ttcgctcctc ggtctgtcgc gccgccgaac cgcgttcgac gtattcggcg
     5581 cgagcctgcc gtgcgttcag acaagcggtt tcgtagtcat cagtcagctt gatctgtcgc
     5641 tcacgcctct cggcaaccat cgctgctagc tggctgccgc gttcaaccag agcctgatgc
     5701 cgctgcgcgg caacgtttag acggccagcg tcacgctccg cc
//
LOCUS       DAOTAC010000159         5736 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_11757_length_5736_cov_4.926069, whole genome shotgun sequence.
ACCESSION   DAOTAC010000159 DAOTAC010000000
VERSION     DAOTAC010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5736
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11757_length_5736_cov_4.926069"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1138
                     /locus_tag="PLM74_07570"
     CDS             <1..1138
                     /locus_tag="PLM74_07570"
                     /inference="COORDINATES: protein motif:HMM:NF024785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HON42969.1"
                     /translation="MDNLHLSGRYYLPADALVRRRIESGLSRTFETRGYYEIIPPMLV
                     GLGLAQFGPDGPGNSGGMSSSCGSKAAGCAEHSGHDLEDECTSYVVLTRDGHLLTLCS
                     DLTTPVAALAARHLLSADGPTRLYYSGTVFRAQKPGSGRPEERRQVGAELIGRGATGV
                     QADAEILSMATEGLINIGLRDFCIGLSDAKLLSAILDAAGAQGDTGERIRSALAARDY
                     VLYGELVSSLDGAAVSVELLRRLPHLHARAEVLEALRTADVCEAGGGLVRTLDALDNR
                     GLGRFFRLDLAITRRFRYYTGVVFEGYAPGCGNAVIGGGRYDDLLACFGASAPAAGFA
                     VDVDSVASALGALDEPKESGGGAGQWGWRLWTNLASCEAVRSRH"
     gene            1078..1818
                     /gene="hisG"
                     /locus_tag="PLM74_07575"
     CDS             1078..1818
                     /gene="hisG"
                     /locus_tag="PLM74_07575"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HON42970.1"
                     /translation="MGLETVDESCVMRSRQVAPLSIALPKGRLLRPSLGILRGIGLIC
                     PDVDELDRRLVYETADSRVRFIIARPADVPAYVDHGAADLGITGKDVLIEGDWNVCEL
                     LDLGFGQCRMAVAAPRAATGPDPAVALSDPRPPRLGHTLRVATKFPGIARRYFAPRRD
                     LCVQIIKLNGAVEIAPIVGLADVIVDIVDTGRTLADNGLVVVEEIMQVSARLIANRAS
                     LQLKSAAIYPVTSALSEHCRQLDDAMAR"
     gene            1847..3145
                     /gene="hisD"
                     /locus_tag="PLM74_07580"
     CDS             1847..3145
                     /gene="hisD"
                     /locus_tag="PLM74_07580"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HON42971.1"
                     /translation="MDTPLTIRTTAAADLLRQGIRRGLEGLDEIEDDVRSIVEDVRNR
                     GDRAVRDYTERFDGAGSVPSNVEVTPCEIRHAYSAVDESVVEALRRAADNIRRFHERQ
                     VQQSWTCILDDGTQMGQVIRPLARVGVYVPGGTAAYPSSVLMGAIPARVAEVGQVIAC
                     TPPGPGGVANPYVLVAADIAGVDRVFKVGGCQAIAAMAFGTDSIPAVDKIVGPGNAYV
                     TAAKKLVFGYVDIDMLAGPSELVVLADQSADARLVAADLLSQAEHGPDSEAILVTTSP
                     ELSAAVERELRIQVDALSRRSTIACSLGRHGLMVIANDLDQAIDVVNHYAPEHLEILV
                     DDPYALLGCIHNAGSVLLGPLTPVAATDYAAGPNHVLPTGGTARSRSALSVRDFVRVQ
                     NVTSLAHASLDDILDAAHTIAAIEGLDAHAAALAARRSTP"
     gene            3142..4224
                     /gene="hisC"
                     /locus_tag="PLM74_07585"
     CDS             3142..4224
                     /gene="hisC"
                     /locus_tag="PLM74_07585"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HON42972.1"
                     /translation="MTANGIRIRLDSNESPFDLPAPLKENVLDQLRTIPFNRYPKRLA
                     ANLKRTIASRIGVDDTQVTLGNGLDEVIHIILQTFGPRRSVIVQRPSFVMYEVAALAA
                     GADLRAVTLGEGLQLDVSGIIEEANAGAWPGPESDPPMVILCNPHNPTGGRLSEADIE
                     AVLRATQALVVVDEAYVEFAGGSVLPLLAKYDHLVVLRTMSKAFGLAGVRVGYSVSSP
                     GIAAELAKVKQPFNLDSLALVIAQAALQDDAYCQETVARIIEERTRLAQRLSCIPSIT
                     CLPSCTNFILFRTESAEAAEVHDALGQRGISVRIFPGEPMLRGYLRVSCGTREEDDIF
                     LAALESVLGDSCSARIRRYSGVREGH"
     gene            4226..4870
                     /gene="hisB"
                     /locus_tag="PLM74_07590"
     CDS             4226..4870
                     /gene="hisB"
                     /locus_tag="PLM74_07590"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006557732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HON42973.1"
                     /translation="MSGTRQAQVHRRTSETDVDVLLGLDSDDSPSSAAPVGTAGADAA
                     AGAPDISAGCGFLEHMLRLLAFHGRLNMKIRAAGDVEVDYHHLVEDVGICMGQALREA
                     LGDKQGIARFGSAIVPMDDALCMAVVDLSGRPYLHFNAPMPCAKVGDFDTELVEEFMR
                     ALANHGQLTIHIDLLHGSNTHHIIEAASKALALALRQAVAIDATQCVPSTKGVL"
     gene            4876..>5736
                     /gene="hisA"
                     /locus_tag="PLM74_07595"
     CDS             4876..>5736
                     /gene="hisA"
                     /locus_tag="PLM74_07595"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HON42974.1"
                     /translation="MLVIPAIDIHDGKCVRLYQGDFAKVTEYSDDPVEVARGWDWLGA
                     EMLHIVDLDGARAGRPVNLDIVLRIALDTGLPIQLGGGLRKTKHIKSALESGATRVIL
                     GTAACGDPDMLRELVRRFGERIIVSIDTHHGRVMTDGWLQSSTIAPEELAARVLEMGI
                     RTIVHTNVSRDGTLSGVDIDTVSSLLSTGVNVIAAGGVGRVQDIRRLKPLERRGLAGV
                     IIGRALYTGAVKLPDALRAAGSRRIIPCLDMKDGRVVKGVAFTHLRDAGDPLELALTY
                     EKEGADELVLL"
BASE COUNT         1128 a         1720 c         1828 g         1060 t
ORIGIN      
        1 catggacaac ctgcaccttt cggggagata ctacctccct gccgacgccc tcgtccggcg
       61 acgtatcgag agcggcctgt cacggacgtt cgaaacccgc ggctactatg agataatccc
      121 accgatgctt gtagggctgg gactggcgca gttcgggccg gatggcccgg gcaactccgg
      181 cggcatgagt agctcgtgcg gttccaaagc agcgggctgc gcggaacact cagggcacga
      241 tctagaggat gaatgcacat catacgtggt cttgactagg gatggccact tgctgacgtt
      301 gtgctctgac cttaccacgc cagtcgctgc cctggcagcg cgccatctgc tgtcggcgga
      361 tgggcctacg cgtctgtatt actcgggcac tgtgttccgc gcccaaaagc cgggaagcgg
      421 caggccggag gagcggcggc aggttggagc ggagcttatc gggcggggcg ccacaggcgt
      481 ccaggcagat gcagagattc tgtcgatggc caccgaaggg ctgatcaaca tagggctgcg
      541 cgatttctgc atcggcctaa gtgatgcaaa actcctttcg gcgatacttg acgccgcagg
      601 cgctcagggc gatacaggag aacgcatccg ttctgccctg gcagcgcgcg actacgtcct
      661 ctacggcgaa ctagtgtcgt cattggacgg cgctgccgta agcgtggaac ttctcaggcg
      721 cctgccccac ctccacgccc gcgctgaggt tctggaggcg ctgcgaaccg ccgacgtatg
      781 cgaagcaggg ggagggcttg tacggactct cgacgccctg gacaaccggg gcctgggcag
      841 gtttttccgt ctagatctgg ccataacgag gcgattccgc tactacaccg gagttgtctt
      901 tgaaggatat gcgcccgggt gcggaaacgc ggtgatcgga ggggggcgct atgacgactt
      961 gctggcctgt ttcggcgcct ccgcgcccgc agctgggttc gctgtggatg tcgattccgt
     1021 ggcatcggcg ctcggcgcgc tggacgagcc taaggagtca ggaggaggtg cagggcaatg
     1081 gggttggaga ctgtggacga atcttgcgtc atgcgaagcc gtcaggtcgc gccactgagc
     1141 atcgcgcttc ccaagggtag gctgctccgg ccgtcgctgg gtattctcag gggaatcggc
     1201 ctcatctgcc cggacgtgga tgaactcgac agaagactgg tgtacgaaac ggcagatagc
     1261 cgtgtcaggt tcattatcgc ccgccccgct gacgtaccgg cttatgtgga ccacggcgcc
     1321 gccgacctgg gcataacggg caaggatgtt ctcattgagg gcgactggaa tgtgtgcgaa
     1381 ctgcttgacc tgggcttcgg gcaatgccgc atggctgtgg ccgcgcctcg agcagcaact
     1441 gggcccgacc ccgccgtggc actctccgac ccaaggccgc cgcgcctcgg ccacaccctg
     1501 cgggtggcca ccaagttccc gggtatagcc aggcgctact ttgcccccag gcgggatctg
     1561 tgtgtccaga tcatcaagtt gaacggggca gtggagatcg cgcccattgt agggctcgcc
     1621 gatgtgatcg tggacatagt cgataccggc cgtacactgg ccgacaacgg tctggtggtg
     1681 gtggaggaaa taatgcaagt atcggcgcgc ctcatagcaa accgagccag cctgcaactg
     1741 aagtctgccg ccatctatcc ggttacgtcg gcgctcagtg aacactgccg tcaactcgac
     1801 gacgcgatgg ctcgatagca ggactgcaat tccgggaggt gttcacatgg acacgccgtt
     1861 gacaatcaga actactgccg ctgccgacct actgcgacaa ggaatccgtc gcgggctgga
     1921 gggtctggac gaaatcgaag atgatgtccg ctccatagtg gaagacgtac gaaaccgcgg
     1981 cgacagggcc gttcgcgact acaccgaaag gttcgatgga gccgggtccg ttccgagcaa
     2041 cgtagaagtg acgccctgcg agatccgcca tgcctactcg gcggtagacg aatcagtcgt
     2101 agaggcgctt cggcgagccg cggacaatat cagacgtttc catgagcggc aggtgcagca
     2161 gtcctggacc tgcatactcg atgacggcac gcagatgggg caggtaatcc gccctctggc
     2221 gcgagttggc gtgtacgtgc ccggcggcac tgccgcctac ccgtcttccg tgctcatggg
     2281 agcaataccg gcccgagtgg ccgaggttgg ccaggtgata gcctgcacgc ccccaggtcc
     2341 cggcggagta gcaaaccctt acgtgctggt ggcggccgac attgcaggag ttgatagagt
     2401 gttcaaagta ggcggatgtc aggccatcgc ggcgatggct ttcggaaccg actccatccc
     2461 cgcagtagac aaaattgtcg gcccgggcaa tgcctacgtc accgcagcca agaagctggt
     2521 gtttggctat gtggatatcg atatgctggc cggcccgagc gagctggttg tgctggccga
     2581 ccagtcggcc gacgcgcgcc ttgtggcggc cgacctgctg tcgcaggctg aacacggtcc
     2641 ggactcagag gccatactcg tgactaccag ccctgagctg tccgcggccg tcgagcgcga
     2701 gctccgcatc caggtcgacg ccttatccag gcgcagcaca atcgcctgtt cgctcggccg
     2761 acacggcctg atggtcatcg ccaacgacct tgaccaggca atagacgtgg tcaaccacta
     2821 cgcccccgaa cacctggaga tcctcgtgga cgatccctac gccctgctcg ggtgtatcca
     2881 caatgccggc agcgtgctct tgggtccgct cacgccggtg gcggccaccg actatgcggc
     2941 cggacccaat cacgtacttc ccacaggagg caccgcccgc agtcggtccg cgctttcagt
     3001 gcgcgatttc gtgagggtgc agaatgttac gtctcttgcg cacgcgtcgc tcgacgacat
     3061 tctggacgcc gcccacacca tagctgccat cgagggattg gatgcgcacg cggcggctct
     3121 cgcggcaagg aggagtacac catgacagcc aacgggatcc gcatcaggct cgacagcaat
     3181 gagagccctt tcgaccttcc cgcgccgctc aaggagaacg ttctggacca gctacggacg
     3241 atcccgttca acaggtatcc caagaggctg gctgccaacc tcaagcggac gatcgcatcg
     3301 cgaatagggg tagacgatac gcaggtcact ctgggcaatg gcctcgatga ggttatccat
     3361 atcatcctac agacctttgg gccccgcaga agcgtgatcg tacagaggcc ttcgttcgtc
     3421 atgtatgagg ttgccgcgct cgctgccggc gcagatttgc gggcagtaac ccttggggaa
     3481 gggcttcaac tggatgtatc cgggatcatc gaagaagcga acgcaggagc atggcccgga
     3541 ccagagtctg atccccccat ggtgattttg tgcaacccgc acaaccccac cggcgggcga
     3601 ctgtcagaag cggatatcga ggcagtgctc agggcgactc aggctctggt tgtggtagac
     3661 gaagcctacg tagagttcgc aggcgggagc gtgctgccgc tgctcgccaa gtacgaccat
     3721 cttgttgtgc tgcggaccat gtcgaaggcg ttcgggctgg ccggagtgcg cgtggggtat
     3781 tcggtatcga gcccaggcat cgcagcggag cttgctaagg tgaagcagcc cttcaacctg
     3841 gactcgcttg ccctggttat cgcccaggca gccctgcaag acgacgcata ctgccaagag
     3901 acggtagccc gaatcatcga ggagcgcacc cggctcgccc agcgtctatc atgcatcccg
     3961 agcataactt gcctgccgtc gtgcacgaac ttcattctgt tcagaacgga gtcggcggaa
     4021 gcggcagagg tccatgacgc gctcggccag cgggggatct cagtgcgcat cttcccgggc
     4081 gagcccatgc tgcgcggata tctcagagtc agctgtggaa ctcgcgagga agacgacatc
     4141 tttctggcgg ctctggagag cgttctgggc gactcgtgct ccgccagaat acgccgctac
     4201 tcaggcgtta gggagggtca ttgaaatgtc cggcaccaga caggcccaag tgcatcgcag
     4261 aacttctgag acagatgtcg acgttcttct tggtcttgac agtgatgata gcccttcttc
     4321 ggccgcgccg gtcggcaccg ccggcgcaga tgccgccgcc ggggctcccg acatctcggc
     4381 aggttgcgga tttctggaac acatgctccg attgctggcg ttccacggtc gactgaacat
     4441 gaagatacgc gcagccggcg acgtcgaagt agactaccac cacctcgtcg aggacgtggg
     4501 catctgcatg gggcaagcct tgcgcgaagc gctcggcgac aagcagggca ttgcgcggtt
     4561 cgggagtgcc atcgtgccga tggacgatgc cttgtgtatg gcggtcgtcg atctgtccgg
     4621 gcgtccttac cttcacttca acgcgcccat gccgtgcgct aaggtaggag acttcgatac
     4681 tgaacttgtg gaggaattca tgcgcgcatt ggcaaaccac gggcagctga ccatccacat
     4741 cgacctgctc cacggctcga acacgcacca catcatagag gccgcgtcca aagccctggc
     4801 gcttgcgctg cggcaggctg tagcgattga cgcgacgcaa tgcgttccgt ccacgaaagg
     4861 ggtgctatag gctcaatgct tgtgattccg gctatcgaca ttcacgacgg aaaatgcgtc
     4921 cgtctgtatc agggggactt cgccaaggta acggagtact cggacgatcc cgtcgaagtc
     4981 gcccgtggct gggactggct aggagccgag atgctacata tagtggacct ggacggagca
     5041 agagcaggca ggccggtgaa tctcgacatc gtactgcgca ttgcactgga tacaggtctt
     5101 cccatacagc tgggcggcgg gctcagaaag accaagcaca tcaaatcggc cctcgaatcc
     5161 ggagcgaccc gcgtcatttt agggactgcc gcgtgcggcg accctgatat gctccgagag
     5221 cttgtccgac gcttcggcga gcggatcata gtgagcatcg acacccacca cggcagggta
     5281 atgacagacg ggtggctaca atcgtcgacc atcgcgcccg aggaactggc cgctcgcgtg
     5341 ctggagatgg ggattaggac tatcgtgcac acaaatgtgt cccgtgacgg cacgctgagc
     5401 ggggtggata tcgacaccgt ctcctccctg ctgtcaactg gcgtaaacgt catcgccgca
     5461 ggcggagtcg gtagagtcca agatatccgt cggctgaaac ctctcgaacg ccggggcctg
     5521 gccggagtga tcatcggccg cgcgctgtac accggcgcag tcaagctccc agatgcgctc
     5581 agagcggcag gaagcagaag gatcatccca tgcctggaca tgaaagacgg tcgtgtggtc
     5641 aaaggagtcg ccttcacaca cctaagggac gccggagacc cacttgagct ggctctgacc
     5701 tatgagaagg aaggcgccga cgagctggtg ttgctc
//
LOCUS       DAOTAC010000160         5708 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_11853_length_5708_cov_4.343004, whole genome shotgun sequence.
ACCESSION   DAOTAC010000160 DAOTAC010000000
VERSION     DAOTAC010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5708
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11853_length_5708_cov_4.343004"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..1432
                     /locus_tag="PLM74_07600"
     CDS             101..1432
                     /locus_tag="PLM74_07600"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HON42975.1"
                     /translation="MWLLDSYVTILVMSYLIGALPAIRLPAFVRPVGIRAPWLSPVLR
                     GMAAVGLARVSVGTPIAITAAGLAVVVGATWSLLVPGRRGLTVPAAVGVGLVISPAAA
                     CASGGIAYIIARALRKRRDMLPLVPTVAGGILYPPLVWLFERFDLHLILAAVLALICI
                     YEALPALPRSAGDRGTRKTQDTSPTLALFRAVMSRMATTLLVIGLAAAILLNRYVYRG
                     FGMGVEIFRRGSNEFSLVALTFDDGPNPEYTPFILDTLKAKGVHATFFLVGEEVERYP
                     DLARRIVEEGHEVGSHTHSHRNMMGLQAHLMKQEIVCSEDAITRACGEPPRLFRPPRG
                     LYDERLIEQLRERHYALALWSISSMDWTEASEASMVHRLSRVVRNGDVILFHDSGGIV
                     ANSGGSRTNTVRALPHVIDRLRDRGFAFATVSQLMFISGLAGDEPPESGGK"
     gene            1434..2072
                     /locus_tag="PLM74_07605"
     CDS             1434..2072
                     /locus_tag="PLM74_07605"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HON42976.1"
                     /translation="MGIRPVSDSDVPTVAGIFIRAFPESVKHYYGASVPPIDGFRDIF
                     AFLARAERGNFLVYEESEGVLGYVVVPKTMGLVWAKALFGGHVFLWAFRWMMGRYGIP
                     FSRVARILGNKLLFAAYSSEQLLHGHAQVLSVAVAPSAQGRGIGRQLVQAGLELLRRQ
                     GVRTVKLEVRPDNEPARRIYSSLGFGEAGKSRDSQGEWMVMIAHLQPDPQSS"
     gene            complement(2101..3240)
                     /locus_tag="PLM74_07610"
     CDS             complement(2101..3240)
                     /locus_tag="PLM74_07610"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HON42977.1"
                     /translation="MSRSQVSVVRCPSYDTARVKASVQELLAPLGGVQSFVSPGQTVL
                     IKPNLIHGTPPERGATTHPEVLRAVIEEVHGAGGRPVVGESPGYESFTTAARKSGLLT
                     VIEELDVPIVPFDTWTEVRLGPGHLVPSLPIAGPVVAADVVISLPKLKSHGLVVYTGC
                     VKNMFGVICGTQKAQYHLRFQDREAFSRLLAEVYSVARPTLSILDGIIGMDGNGPRNG
                     DPAPLGIMLAGADGVAVDAVACRIVGIDPLESPPIRIAAQMGLGTASLRQIDLAGPPI
                     EELKTTGFQAPRSMRPSIRQRAMSGRAGIILRNLFTTRPTVHPGGCRGCTICARACPA
                     QAIEIRDGKAQMDYSKCVRCYCCQEVCEYGAIRLATPPLARLLHR"
     gene            3422..4153
                     /locus_tag="PLM74_07615"
     CDS             3422..4153
                     /locus_tag="PLM74_07615"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HON42978.1"
                     /translation="MELDRDSRTPLYQQIYEIWRKRIESGEVRPGERIPTERELCDIY
                     EVSQITVRQAIQMLVTEGLLVRRPRTGTRVAHRKFSQDLIRLTSFSEDMRSRSLRPGA
                     MVIRTCEETVDVRVAEKLKLSDRAAVTRLERLRLAEDEPMAIETCHIPVALCPGLAER
                     NLEGVSLYDLLRSEYRVDMQWAQQSFEASSATSAEARLLGIKRGAPVLRTERLTFDST
                     GRPVEFAVSVYRGDRYKLNVQMRRV"
     gene            4182..>5708
                     /locus_tag="PLM74_07620"
     CDS             4182..>5708
                     /locus_tag="PLM74_07620"
                     /inference="COORDINATES: protein motif:HMM:NF039707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HON42979.1"
                     /translation="MLQAGDGRIRISIDEFSGAIAEIRDLKVSDDLIKYPSASVGAPL
                     GIVCLDRQGRRREYTAGPVTRAQDSPGNRLLICHDHVVETSRSSPNGPGRADISCRWR
                     LDAEAGPEGTSSWTIRVENNAEDLTVVEVLFPVVRGIQLGQNHADEVLVFPHHAGEKI
                     VDPALTIASDRYMRFWRAGTVRGADGIYSREIDYCGLASMTWMDLYSPAPNGGFSGLY
                     MASYDPSFVLTGVRSESGGPDFPWCGLGFRKYVPVAPGECWASAPCIIAVHDGDWHWG
                     AAQYRRWILEHISLSDVPSDLAMESAVCPRYDFKQNQSICHRYTEIPEMYEQARAEGI
                     THFFISAWNRQGFDTDYPEFVPDMELGSSWNLARGCEYVRDHGGFSTFYINVRLFDLE
                     SDFFPTLGRTWAIKNHDGAMFHETYGPRMFAVICPDCEEWRKWVADTAGWMVKAFGAR
                     GIYLDQLGSAEPFPCYGAGHAHSGRSPAHHGRYNHGYLKMIREVRDRTRDLDPSSFLM
                     I"
BASE COUNT         1109 a         1612 c         1792 g         1195 t
ORIGIN      
        1 ccgatggtac ttggcgggag accgcccggg agagtaggtc gccgccggat ttttttattt
       61 cctcaaagcg cagcgcatcc aggtaggagc tgaagcggca gtgtggcttc tcgactcgta
      121 cgttactatt cttgtgatgt catacctgat cggggcgttg ccggctattc gcctccccgc
      181 gtttgtgcgc ccggtgggca tccgcgcgcc ctggctttcg ccagtgctcc gtgggatggc
      241 cgcggtgggc ctcgcccgtg tgtctgtagg cacgcctata gccatcaccg ccgccggctt
      301 ggctgtggtc gtaggagcga catggtcatt gctagtcccc ggtcggcgag gcctaaccgt
      361 ccctgccgcg gtgggcgtgg ggctagtcat ctcgccggca gcggcatgtg cgagtggcgg
      421 cattgcgtac atcatcgcta gggcactgcg caagcgccgc gacatgctac cgctggtacc
      481 tactgtggct ggggggattc tgtatcctcc tctggtctgg ctgttcgagc ggttcgacct
      541 tcatctgatt ctcgcagcgg ttctcgcgct gatttgcata tacgaggcgc ttccagcttt
      601 gcctcgctcc gccggagacc gggggactag gaagacgcag gatacgtccc caacgcttgc
      661 acttttccga gcggtaatgt cgagaatggc aacgacactg ctggtcatcg gccttgcggc
      721 tgcgatcctc ctaaaccggt atgtatatcg cgggttcggt atgggagttg aaatcttcag
      781 acgaggctcg aacgagttca gcctggttgc cctcactttc gacgacggcc caaatcccga
      841 atacaccccg tttattctcg atacgctgaa ggccaagggg gtccatgcta cgttcttcct
      901 cgtcggtgag gaggtggagc gatacccgga ccttgctcgc cggattgtag aggaaggtca
      961 cgaggtcgga tcacatacgc actctcatag aaacatgatg ggtctgcagg cccacctgat
     1021 gaaacaggag atcgtttgct cagaggacgc aataacccgg gcatgcggag agccgcccag
     1081 gctcttcagg ccgcctcgtg gcctatacga tgaaaggctg attgaacagc ttcgcgagcg
     1141 tcactatgcg ttggcactgt ggtccattag ctctatggac tggactgaag cgtcagaggc
     1201 aagcatggtc catcgcttat ccagggtagt caggaacggg gatgtcatct tattccatga
     1261 cagcggcggc attgtagcca atagcggagg gtcgagaact aacacggtgc gagctttgcc
     1321 gcatgtcata gatcgactca gagaccgcgg ttttgcattc gccacggtgt cacagctgat
     1381 gttcatatcg ggcctcgcag gagatgaacc gcccgaaagc ggaggcaagt gacatgggaa
     1441 tacgtcctgt gagtgattcg gatgtaccga ccgtagccgg catcttcatc cgggcatttc
     1501 cagaatcggt gaagcactac tacggagcga gcgtcccgcc gatcgatggg tttcgcgata
     1561 tcttcgcatt ccttgcgcgg gcagagcgcg gaaacttcct tgtgtacgag gagtccgaag
     1621 gggtactcgg ctatgttgtc gttccgaaaa ccatgggact agtgtgggcc aaggctctct
     1681 tcggaggcca tgtgttcttg tgggcattta ggtggatgat gggaaggtac ggcatcccct
     1741 tcagccgtgt cgcaaggata ctcgggaaca agctgctttt tgccgcgtac tccagcgagc
     1801 aattgcttca cggacatgct caggtcttat ccgtggccgt ggccccaagc gcccaggggc
     1861 gggggatcgg caggcagctg gttcaggcgg ggctggagct cctgcgccgg caaggggtcc
     1921 gcactgtgaa actggaggtg aggcctgata acgagcccgc cagacgcata tactcgagcc
     1981 ttggctttgg cgaggcgggc aaatcccgcg acagccaggg cgagtggatg gtgatgattg
     2041 cccacctgca gccggacccg cagtcttcct gaatcccggc gctgatactt ggcaccggcc
     2101 ctaccggtgc agaagccttg caagaggcgg cgtcgcaagc cggattgcgc catattcgca
     2161 tacctcctgg cagcaatagc aacgtacgca cttgctgtag tccatctgcg ccttcccatc
     2221 acggatctct atcgcctgcg cggggcaggc cctggcgcat atcgtgcatc cacggcaccc
     2281 gcccgggtgg acggtgggcc ttgtggtgaa caggttcctc aggataatac ctgccctccc
     2341 cgacatggct ctctgccgaa tggacgggcg catgctgcgt ggagcttgga aaccggtagt
     2401 cttcagctct tcgatgggcg gtccggccag atcaatctgg cgcagggagg ccgttcccag
     2461 gcccatctgg gccgcgattc tgataggcgg cgactcgaga ggatctatgc ccactattcg
     2521 gcaggcgact gcgtccacgg ccactccatc tgcacctgcg agcattatgc ccaggggtgc
     2581 cggatcgccg ttacgcggac cattgccgtc cattcctatt atgccatcga gtattgagag
     2641 ggtaggccgg gcgaccgagt acacctccgc cagcaaccgc gagaacgcct cgcggtcttg
     2701 gaacctcaga tgatactggg ctttctgcgt tccgcagatt acgccgaaca tgttcttcac
     2761 gcatccggtg tagactacca ggccatgcga cttgagcttg ggaaggctga tcacgacgtc
     2821 tgctgccacc accgggccgg ctatgggtag agaagggacc aggtgccccg gtcccagccg
     2881 gacttcggtc caggtgtcga agggtacaat aggtacatcg agttcctcga tgaccgtgag
     2941 gagaccggat ttccgggcag ccgtagtgaa gctttcatac cctgggcttt caccgaccac
     3001 tggcctcccg cctgcgccgt gcacctcttc aattactgct cgaagcactt cagggtgggt
     3061 agttgcgccg cgctcaggcg gcgtgccgtg gatcaggttc ggctttatca ggaccgtttg
     3121 gccgggcgat acgaaggatt gcacgccgcc tagaggcgcc aacaattcct gtactgatgc
     3181 tttcacgcgc gcggtgtcgt agctcggaca ccttacgaca gagacttgcg atctgctcac
     3241 tttgccacat cacttcctgt acagccttat acacttgata cgtgtcggtg tcaacagcaa
     3301 tgactggtgc gtggtgcaca tagcgaaacg agcttgccat tgattataaa gaatagctat
     3361 aataagctaa tgggacttca cgatacgatg catattgtgg tttgataggc ggtggtgcga
     3421 agtggagctg gacagggatt cacgtacccc tctttaccag cagatctacg aaatatggcg
     3481 caagcgcatc gaaagcggag aggtgcggcc tggcgagagg attccgaccg aacgggaact
     3541 ctgcgatatc tatgaggtga gccagataac ggtccgccag gcgatacaga tgttggtgac
     3601 cgagggtcta ctggtgcgca ggccccgcac aggcactcgc gtggctcacc gtaagttcag
     3661 ccaggacttg atcaggctta ctagcttctc ggaggacatg aggtcgagga gcctgcgtcc
     3721 gggggcaatg gtgatccgta cctgcgaaga gacagtcgat gtcagagtgg cagagaagct
     3781 gaagctcagc gacagggcag ccgtgaccag actcgagcgc ctgcggctcg ccgaggatga
     3841 gccgatggcg atcgagacct gtcacatccc ggtagccctg tgcccagggc ttgctgaacg
     3901 taacctcgaa ggggtgtcgc tctacgatct gcttcggtcg gagtacagag tggacatgca
     3961 atgggcgcag cagagttttg aggcttcatc agcgactagc gccgaagccc gcctactcgg
     4021 cataaagcgc ggggcccctg tgctccgcac cgagcgcctc accttcgact caaccggcag
     4081 gccggtggag ttcgcagtct ccgtctatcg tggcgacagg tataagctga acgtgcagat
     4141 gaggcgagtg tagcgcgggg tcttaggagg gaggatccgg catgctgcag gcaggagacg
     4201 gacgaatccg catctcaatc gacgagttca gcggggctat cgccgagatc cgggacctta
     4261 aggtatctga tgatttaatc aaatacccat cggcctcggt gggggcgccg ctcggcattg
     4321 tctgcctgga caggcagggc agacgccggg agtacacggc agggccggtg acccgagcac
     4381 aggattcgcc gggcaatcgt ctgctcatat gccacgatca cgtagttgaa accagccgca
     4441 gctcgccaaa tggcccgggg cgcgcggaca tctcgtgccg ctggcgcctt gacgcggaag
     4501 cgggacccga ggggacttct tcatggacga taagggttga aaacaacgcc gaagatctga
     4561 ccgtggttga ggttctcttc ccggttgtgc gaggtatcca actaggccag aaccatgccg
     4621 acgaagtgct tgtcttcccg catcacgcgg gcgagaagat tgtcgatcct gcccttacga
     4681 tcgcctccga tcggtacatg cgattctggc gagcgggcac ggtgcgtgga gccgacggca
     4741 tctactctcg cgagatagac tactgcgggc ttgcttccat gacatggatg gacctataca
     4801 gcccagcccc taatggcggg ttctctggat tgtacatggc atcgtacgac ccatcctttg
     4861 tactcaccgg ggtgcgcagc gagagcggcg gcccggactt cccctggtgc gggctggggt
     4921 ttcgcaaata cgtgcctgtg gcacccggcg agtgctgggc gtccgcccct tgcatcatag
     4981 cggtacatga tggcgactgg cactggggcg cagctcagta tcgccgctgg attctggagc
     5041 atatcagctt gtccgatgtc ccttctgatc tagcgatgga gtcggcggtg tgcccacgat
     5101 atgactttaa gcagaaccaa tcgatatgtc acaggtatac cgagataccg gagatgtacg
     5161 agcaggccag ggccgaggga atcacacact tcttcatctc agcctggaac aggcagggct
     5221 tcgacaccga ctatccggag ttcgtgcccg acatggagtt gggcagctcg tggaacttgg
     5281 cccgcggatg cgagtacgtg cgtgaccatg gcggtttttc gaccttctac atcaacgtgc
     5341 gcctattcga cctggagtcc gacttctttc cgactctggg gcgaacgtgg gccatcaaga
     5401 atcacgacgg agcaatgttc cacgaaacgt acggcccgcg catgttcgct gtgatatgcc
     5461 ctgattgtga ggagtggcgg aaatgggtgg cggacactgc aggatggatg gtgaaggcct
     5521 tcggggccag gggcatttac ctggaccagc tgggatcggc tgagcctttt ccgtgctatg
     5581 gagcggggca tgcccactcc gggcggtctc ctgcgcatca cggccggtac aaccatggct
     5641 acctcaagat gatccgcgag gtgcgggatc ggacgcgtga tctggacccg tcatcgtttc
     5701 tgatgatt
//
LOCUS       DAOTAC010000161         5678 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_11936_length_5678_cov_5.136760, whole genome shotgun sequence.
ACCESSION   DAOTAC010000161 DAOTAC010000000
VERSION     DAOTAC010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5678
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11936_length_5678_cov_5.136760"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="PLM74_07625"
     CDS             <1..392
                     /locus_tag="PLM74_07625"
                     /inference="COORDINATES: protein motif:HMM:NF015426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42980.1"
                     /translation="TMEDAADLTREFVDRFGPLPSPVSNLITIAAIRVLARELGVMAI
                     AGERNSVIVRFPEGVRYASQDIVRIARPFKGRVMLGAGRSQTIRVKTQGMPDREILDT
                     LVGVLTEMRQRAAAVDRVTSTQEAIDG"
     gene            389..943
                     /locus_tag="PLM74_07630"
     CDS             389..943
                     /locus_tag="PLM74_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011229564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation T C-terminal
                     domain-containing protein"
                     /protein_id="HON42981.1"
                     /translation="MRPTGIVRRIDELGRIVVPKELRRSLRIHEGDSVEIFVDPDGSI
                     VLKKYSAVGQLKEMAVDMSEALAKSSGEVALICDRDVVIACAGAGEHDLSGRAVGRAV
                     EKSMLERQVLLVHFSGGSESSSILVDDGSEFALISAAIAPVIADGDPVGAVIIGTTSQ
                     QKTVGDLEESLVVTAAGYIGRQVE"
     gene            984..1781
                     /gene="mazG"
                     /locus_tag="PLM74_07635"
     CDS             984..1781
                     /gene="mazG"
                     /locus_tag="PLM74_07635"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HON42982.1"
                     /translation="MADQCRYPADRLIDIMKRLRGADGCPWDRKQTSSSLKPYVIEEA
                     YEVVEAIDNGDPGKLCEELGDLLLQIAFHCQIASEQAAFNFNDVVDAICEKLIRRHPH
                     VFADVEADDAGTVLRNWERIKQKEKESDSGTPASILDEVPAYLPALMQAAKMQDKASR
                     VGFDWQYAEEAAAKVWEETDEFREALASGRRDRIRDEMGDVLFAVVNVARLLDMDAEE
                     VLRRANGRFVDRFRYIERWADERDVRLEDMTLGEMDAVWNEAKKAGL"
     gene            1905..2216
                     /gene="yabP"
                     /locus_tag="PLM74_07640"
     CDS             1905..2216
                     /gene="yabP"
                     /locus_tag="PLM74_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HON42983.1"
                     /translation="MAAKGPDVQEEIRRKRPGLQHKLVLVDRESMTVEGVMNVESFDD
                     EEIVLETNAGVLTVVGHDLHIRQLSLEEGVIEIDGYVESCDYTDENPGRSRGILARLF
                     R"
     gene            2231..3286
                     /locus_tag="PLM74_07645"
     CDS             2231..3286
                     /locus_tag="PLM74_07645"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HON42984.1"
                     /translation="MSRHVAQETQRGPERAAPRRSRPRRSKPPAWRRSGPGLGFAVVT
                     GVLGVLISPVVLLVSIFRPNMRVGKQWVISIAVLTAICVCVAFAAASQIERWSADAVR
                     VMGAYDTARVRVRDLPFAEKVNFEGLRNGVDPSLLAAVMSQSSGFQTDAVSWRGARGL
                     MQLMPAVWEEFMPDAECRGDHAPPACSEMCIYSVDANLRVGAAYLRWLIDANGGDIVA
                     ALAAYSADQPGDYGGSGEMPDSIPADRPLGYVRSIIQAWINYRGEQGRISATQVVTAT
                     TVRTWAGWAAIGLLCLLAVWAALRYPAVGRPLEADSQASIQQPEQGEECAGGDPYVAT
                     AGDARVSGSGDADGAGL"
     gene            3710..4036
                     /locus_tag="PLM74_07650"
     CDS             3710..4036
                     /locus_tag="PLM74_07650"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HON42985.1"
                     /translation="MSSKLTVVLVMLLTVCVGLTYIGGRMRRAALERETEKLLEECRG
                     WELRLDELKKEINVAGTNDYVERAAREKLGLVKPGETLYIVTQPDASGFVPVQRRPGK
                     PIEIND"
     gene            4167..4565
                     /locus_tag="PLM74_07655"
     CDS             4167..4565
                     /locus_tag="PLM74_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007506059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HON42986.1"
                     /translation="MSSIEVGSVLEGRVTGITRFGAFIDIGEGRTGLVHISEIADAFV
                     HDVNDYLKHNDVVRVKVIAVDSGKVGLSIKQADPNYLAQRARRDRAYQQSFEDKLTKF
                     LKDSEERHQDLKRNQDSKRGGRGTRLSRMV"
     gene            4665..5378
                     /locus_tag="PLM74_07660"
     CDS             4665..5378
                     /locus_tag="PLM74_07660"
                     /inference="COORDINATES: protein motif:HMM:NF016314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HON42987.1"
                     /translation="MNSGAWDRAEIRDLAVAARQLGRPLAGRVVAVAKRCSYGQPQVL
                     VTYPLLQVTEYGVAEQNAAEQNAAEHSGDTGPCCEPDHGVPPRWAPFPTVFWLTCPHV
                     RSAVATLESQGMIEDVRRRIREDAEFRREYECANARYASQRIGLLDAADRARLHIEAQ
                     KDGPGAGVLRVIEKSGIGGSADWTGIKCLHMNVADSMAGNANPVGELCVQRLTEDGAG
                     PGLECAGGRCVLARGSLAP"
BASE COUNT         1226 a         1418 c         1929 g         1105 t
ORIGIN      
        1 ataccatgga agatgcagct gacctcacac gtgagtttgt cgacagattc ggccctctgc
       61 cctcgccggt atcgaacctc ataacgattg ctgccattag agtgcttgca cgcgagttgg
      121 gtgtcatggc gatcgcgggt gagcgcaact cggtgattgt gaggttcccg gaaggtgtgc
      181 gttacgcaag ccaggatatc gttaggatag cgaggccttt taagggcagg gtgatgctgg
      241 gcgccgggag gtcgcagact atcagggtca agacgcaggg aatgccggat agggagatac
      301 tagacacatt ggttggggtg cttacagaga tgaggcagag agcagcagca gtggaccgag
      361 taacaagtac acaggaggcg attgacggat gaggcccacc ggtattgtga ggcgtattga
      421 tgagctcgga agaatcgtgg tgcccaaaga gctcaggagg agtttgcgga tacacgaggg
      481 ggactcggtc gagatattcg tggatccgga tggcagtatc gtgctcaaga agtactctgc
      541 tgtcgggcag ctcaaggaga tggctgtcga catgtcagag gcactggcga agtcgtccgg
      601 cgaggtggcc ctgatatgcg acagggacgt agtgatagcg tgtgccggcg cgggcgagca
      661 tgatttgtct ggccgggctg tgggtcgggc agttgaaaag tccatgctgg aacggcaggt
      721 actgctggtc cacttcagcg gcggaagcga gtcgtcttcg attctcgtag acgacggcag
      781 cgagttcgcg ctgatctcgg cggccatagc tcccgtaata gcagacggcg accccgtggg
      841 cgccgtgatc attggtacaa cgtctcagca gaagaccgta ggcgatctcg aggagtcgtt
      901 ggtggttaca gcagcaggat acatcggtcg gcaggttgag tagcgacaga gcctgcgcga
      961 tacggacagg aggcgccggc gccatggcgg atcagtgtag atatcctgcc gaccggctca
     1021 tcgacatcat gaagcggctt cgcggagctg acggttgccc gtgggatcga aagcagacgt
     1081 catcatcgct gaagccgtac gtgattgagg aagcgtacga ggtcgtcgaa gcaatagaca
     1141 acggagaccc gggcaagctc tgcgaggagc tgggcgacct cctgctgcaa atagcgtttc
     1201 attgccagat tgcgagtgag caagctgcgt tcaacttcaa cgatgtcgtg gacgctatct
     1261 gcgagaaact catcaggcgg catccgcatg tgttcgcaga tgtggaggct gatgatgccg
     1321 gcacggtgct gcgaaactgg gagcgcatca agcagaaaga gaaggagtcg gatagcggca
     1381 ctcccgcgtc gatactagac gaagtccccg cctacctccc ggctttaatg caggcggcca
     1441 agatgcagga caaggctagc cgggtgggtt tcgactggca atacgcagag gaagcggcag
     1501 ctaaggtgtg ggaggaaacc gacgaattcc gggaggcgct ggcgtcgggc aggcgcgacc
     1561 ggatccggga tgaaatgggc gatgtcctgt tcgcagtggt caatgtagct cgtctcctgg
     1621 atatggacgc agaggaagtg ctgcgacgtg ccaacggccg gtttgtcgat agattccgct
     1681 acatagagcg ctgggctgac gagcgtgacg tgcgtcttga agacatgact cttggcgaga
     1741 tggacgctgt atggaatgag gccaagaagg cagggctgta gagccacgtt cgccctcgcc
     1801 ttcgtctctg cgtgggctgt tacttgtgcg tgttacttgt gcgcatcatg tgcctcagat
     1861 cggcatagac atccatagga ccattgtcca agggggtgct gtctatggcc gccaaggggc
     1921 cagatgtgca agaagaaatc agacgaaagc gcccggggct tcagcacaag ctggtgctgg
     1981 ttgaccgtga gagcatgacc gttgaagggg tcatgaacgt tgaaagcttc gacgacgagg
     2041 agatcgttct cgaaaccaac gccggcgtgc tcaccgtggt cggccacgat ctccacatca
     2101 ggcagctcag cctggaggag ggcgtcattg agatcgatgg gtacgtggag tcgtgcgact
     2161 acacggacga aaatcctgga aggtccaggg ggattttagc gaggttgttc agatagacgg
     2221 aggacgctag atgtcacgtc acgtagcaca ggagacgcag cggggcccag agcgtgcggc
     2281 gccgcgccga agtaggcccc ggaggagcaa accgcctgct tggcgacgga gcggaccagg
     2341 gctgggcttt gctgttgtaa ccggcgttct gggagtcctg atcagcccgg tagtcctact
     2401 tgtgtcaatt ttccgcccaa acatgcgagt cggcaagcag tgggtgattt ccatcgccgt
     2461 gctcactgcc atctgcgtat gcgtggcatt tgcagccgcg tctcagattg aacgatggtc
     2521 ggccgatgcc gtgagagtaa tgggcgccta cgacacggcc cgcgtccggg tccgagacct
     2581 tccgttcgcc gagaaagtga acttcgaagg actgcgcaat ggggtcgatc catcgcttct
     2641 tgcagcagtg atgtcccaaa gcagcgggtt tcagacagat gcagtgtcat ggcgcggagc
     2701 gcgtggtctc atgcaattga tgccggcggt gtgggaagag ttcatgcccg acgccgagtg
     2761 tcgaggcgac catgcaccgc ccgcgtgctc cgagatgtgc atctacagcg tggacgccaa
     2821 tctgcgtgta ggcgccgctt atctgcgttg gctgatagac gcgaacggag gcgacatagt
     2881 ggccgcgctg gcggcctaca gcgcagacca accgggcgac tacggtggct ccggcgaaat
     2941 gccggattcg atacctgccg atcggccgct gggttacgtc aggagtatca tacaggcgtg
     3001 gatcaactac cgcggggagc agggacggat ttcggccacg caagtcgtga cggcgactac
     3061 ggttcgcaca tgggcaggat gggcggccat cgggctcttg tgcttgctgg ctgtgtgggc
     3121 agccctgaga taccctgccg tcggtcgacc gttggaggcg gacagtcaag catcaatcca
     3181 acagccagag cagggggagg agtgcgccgg tggagaccct tacgtggcaa ctgcgggcga
     3241 tgctcgcgtc tctggcagcg gggatgctga tggggctggt ctttgacatt tccacatttc
     3301 tcaggcggct gatgggttcg ggaaggctga cgacggccat cacggacctg tctttctggc
     3361 tgttttcggc tgcgatgaca ttcgccatgc tcatgcaggc caaccgcgga gaggttcgca
     3421 tgtttgtgat agcggggata gcgggggggt tctgggtcta ctcgttgacc ataaggcatg
     3481 gcgtgttccc cattctgttg tggtgtgaat gtgcaatacg aagaacgggg cactgggcga
     3541 ggcgcagttg ccggtggggg cgcagagctg tcggtggaat gagtcgcgca gttcagaagg
     3601 gatttctgaa aacgcggcga acactatcgc agttacacag cccaaaaccc cggtccgggc
     3661 agggcagcca ggaccgataa tgggcgtatg ctgcgaagga ggccgccgta tgtcttcaaa
     3721 gctcactgtt gtgcttgtga tgttactcac agtgtgcgtt ggcctgacgt acattggtgg
     3781 gcggatgagg cgcgcggctc tggagcgcga gaccgagaag ttgctcgagg agtgcagggg
     3841 gtgggaactt aggcttgatg agctcaagaa agagataaac gtagcaggca ccaacgatta
     3901 cgtggaaagg gccgcccgag agaaacttgg ccttgtcaag ccgggggaga ctctgtatat
     3961 cgtcactcag cctgatgcga gcggattcgt tccagtgcag aggcgcccag ggaaaccaat
     4021 agagatcaac gactagaatc gggctgcaaa tcgcgttccc agtcgtgttc cattgattga
     4081 cactacgcca agcgctcgtg tataataaaa acaacattgg tcggggcgaa atcccggatc
     4141 atcaaaacag gaggatctgt cttagcatgt cgtcaattga ggtcggctca gtcttggaag
     4201 gccgcgtcac aggaatcacc agattcggag cattcatcga tattggtgaa gggaggacgg
     4261 gcctggttca catttctgag atagccgacg cttttgttca tgatgtcaat gactacctga
     4321 agcacaatga cgtagtaaga gtcaaggtca tagctgttga tagcggcaaa gtcgggcttt
     4381 ccatcaagca ggcggatccc aactacctcg ctcagagggc tcgtcgcgac cgggcatatc
     4441 agcagtcctt tgaagacaag ctcaccaagt tcttgaagga cagcgaagaa cgtcatcagg
     4501 acctcaagcg caaccaggat tctaagcgcg gaggaagggg aacccgtctt tccagaatgg
     4561 tttgagaact ggcatcgaca tagccacagc actccctgag acggagtgct tttcttttgg
     4621 accaggccgt gaagacatgt gacacgatgc gggggggcga cggggtgaac agcggagcgt
     4681 gggatcgggc cgagattcgc gatctggcag tggccgcacg gcagctaggt aggcctcttg
     4741 cgggccgtgt tgtggccgtg gccaagcgct gctcctacgg acaaccacaa gtcctagtca
     4801 cttacccgct gctgcaggtc acggagtatg gcgtagcgga gcaaaacgca gcggagcaaa
     4861 acgcagcgga gcactccggc gataccgggc catgctgcga accagaccat ggcgttccgc
     4921 cgagatgggc cccgtttccc acagtcttct ggctcacatg cccccacgtg cggagcgcgg
     4981 tggcgaccct tgagagtcag ggcatgatag aagacgttcg gcgcaggata cgcgaggatg
     5041 cggagtttcg gcgcgagtac gaatgcgcta acgccagata cgcttcgcaa cgaatcggat
     5101 tgctggatgc agctgatcgc gctcggctgc acattgaggc gcagaaggac ggaccgggcg
     5161 ccggtgtgtt gcgggtgatt gagaaatctg ggattggcgg ctcggcggac tggacgggca
     5221 tcaaatgcct gcacatgaat gtagccgact ccatggctgg caatgctaac cccgtgggcg
     5281 agctgtgcgt gcagaggctg accgaggatg gcgcagggcc gggactcgaa tgcgctggcg
     5341 gccgatgcgt gctggcacgg gggtcgcttg cgccgtgatc ccggcagaca tcacaatgag
     5401 cctgcatgta gtgattgaag gaggcgccgt taccggcgcg aattctgaca actcgtacct
     5461 accagagcac ccaggacctg agtaggtcgc tcaaaaggct taccagaaaa cgagtaggat
     5521 ggcctagatt ctggttgggc caaatcggcg ggtgacgagt gcgtgtaggt tttgtgggca
     5581 atggcgattg gagaggcagg taggcatcgc cagagcggat ggtgagccgg aaaatgacat
     5641 cctctccgat cattacagtg ggttgttggc tgcggggg
//
LOCUS       DAOTAC010000162         5662 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_11983_length_5662_cov_4.809881, whole genome shotgun sequence.
ACCESSION   DAOTAC010000162 DAOTAC010000000
VERSION     DAOTAC010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5662
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11983_length_5662_cov_4.809881"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..618
                     /locus_tag="PLM74_07665"
     CDS             52..618
                     /locus_tag="PLM74_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42988.1"
                     /translation="MTTSLCIIAYLAVIAPLIVLVHELSHVVAAIAFVPHDVIVRIGV
                     RPRIQLLKWGRLQVLAQWWGGWVGTWSWAADEVEVGKARYIWIVLAGPVASLLTALLC
                     ASLKNALQNGPRIAYALADTARVGAIATFACTMIPMKYPSWMYGQSGVTSDGYRIWHC
                     LVQKGSGSVLIKRSGHVEAVDRGQERGR"
     gene            732..878
                     /locus_tag="PLM74_07670"
     CDS             732..878
                     /locus_tag="PLM74_07670"
                     /inference="COORDINATES: protein motif:HMM:NF014398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HON42989.1"
                     /translation="MLVCAQCHVEYYFAEPGNGLTFPRAQGTTPAAAVTATQMPTWQD
                     CAPA"
     gene            complement(1175..2050)
                     /locus_tag="PLM74_07675"
     CDS             complement(1175..2050)
                     /locus_tag="PLM74_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42990.1"
                     /translation="MNGAVPQTPNEVRRYSFLMVHEYSHLVHALHARATYPALAEGFA
                     SLAPEYARRDGLKRAGELREATADDADDADDAQEDQWVLPPHITAASALEAGILPPIR
                     QVLTLVHSRQSWNQWMLYSVPTSLLGFIDEEYGRPTLTDIFRHCPENPGPIGQAADIG
                     SAHKELFGTFERYTGVPLDELEAQWHRRLAEMNIDSSSVAAVKLLKDLDRTEMTSLIW
                     ALGTGGKKLDPNFLQTFAELQADIVRFGSLSVSAATPDSDTTADGARLTEESLSERID
                     HLVEWARELWRKAYE"
     gene            complement(2019..2489)
                     /locus_tag="PLM74_07680"
     CDS             complement(2019..2489)
                     /locus_tag="PLM74_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42991.1"
                     /translation="MNPGGKVYVSGYQEDSIRSKAIILARAIRRFGIARLAAVAMVLM
                     LWGAGAAASAAVAQRTVDQVTRYTSIGNDRVILLIPDDICPSEETQQLLLHTATESLR
                     YAEDFFGSRLSHPVVHLWLNAVDKPGSVTPPDGGIRAGDLQGDRRERSSPPNAE"
     gene            2818..4584
                     /locus_tag="PLM74_07685"
     CDS             2818..4584
                     /locus_tag="PLM74_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HON42992.1"
                     /translation="MRRLGLAVLVCLVLAASVTAAPFVEGEDLTPATKPVRGGTLYLA
                     LTASPQSFLFYGSLDNNAYTVIGQTMTGLVELHPVTNAIRPGLAESWETSPDGKEVTF
                     HLRDVKWSDGVPFTADDVIFTFENFIMNKVAKGNSVDRFTILDTRDGQKKEVKWVKVN
                     DKTVKAVLPSPFGPFYMNLSHAYIYPRHKLESKIDKNRPDSVNELWLTNVSPKEIVAN
                     GPYRIASYVMDQKVVLERNPHYWRVDRFGNQLPYFDKLEYLIVKDAEVRLAKFIAGEI
                     DYMAVSAADFPTLKQKELAGGPFTIFKAQPTQPTPSPVHIAFNYDVANEQLKGIFRNT
                     EFRQAMEFLLDRERIIDEVYNGLAIPGAGLVLPANKAFYNPKIEKIMRPYDPAKAKAM
                     LDKLGLKDKNGDGIREFANGKNVEFTLTVQSTPQDYQDVALVFKEDLEKVGIKVNLQI
                     LDSTLVGNMFGAGNFEVGIRAFGNQPDLELRKAIWQPGTQLYYWHYSALDRDKLAAIP
                     ANMLDWEKRVYELFDLGSITTDPAKRKAIYDEVQEIYTDVVPVIFVCKGMNLSGANKS
                     LGNVTQDKEGIVYFATYTAFRK"
     gene            4685..5653
                     /locus_tag="PLM74_07690"
     CDS             4685..5653
                     /locus_tag="PLM74_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HON42993.1"
                     /translation="MWAFTARRLLIMVPMMIVISVICFGITELQPGDFVSQYLDNPRI
                     SPEQIRLLRERLGLDKPAYVRYIMWIKNIVTKWDFGYSFAYERPVGELIFERMGWTVF
                     IALATIVFQWILAVPMGIYSALHPYSPADYAMTVVGFIGISIPDFFFALILMYFMLRM
                     GSTSIGGLFSTRFIGAPWSFAKLIDLLKHLWMPLVVVGISGVAGLMRVMRGNMLDIVG
                     SPFITSLRAHGLDEKTVRRHAIKNALNPMVSIAGAELPNIFSGTIITAIVLNLPTMGP
                     FFYNALLNHDQYLVMSFLMFIALITQIGNLLADIALAFLDPRIRMS"
BASE COUNT         1188 a         1587 c         1705 g         1182 t
ORIGIN      
        1 cctagagaat gaagtgtttg gatgatctct acttgtggag gtgacttcga ggtgaccacg
       61 tctctatgca ttatcgccta cctggcggtg atcgcgccgc tgattgtgct cgtccatgag
      121 ctgagccacg ttgtggctgc cattgccttc gtgccgcacg acgtcatcgt ccggatagga
      181 gtacgcccga ggattcagct actcaagtgg ggcagactgc aggtgctggc tcagtggtgg
      241 ggcggatggg tagggacctg gagctgggcc gcagatgagg ttgaggtcgg caaggcgcgt
      301 tacatttgga tagtgcttgc ggggcccgtc gcatcgctcc taacagcgtt gctctgcgcc
      361 tccctgaaga acgctcttca gaacggcccg agaatcgcgt atgctctggc ggatacggcg
      421 agggtagggg caatagctac ttttgcctgc actatgatac cgatgaagta tccgtcatgg
      481 atgtacgggc agtcgggtgt gaccagtgat ggctaccgca tatggcactg ccttgtccaa
      541 aagggcagcg gatcagtact catcaagaga tccgggcatg ttgaagccgt agatagaggc
      601 caagagagag ggcggtgaac tgcaatgagg aaggccacgc cggcggcggt gtgcattgcc
      661 gtggtggtgc ccgcaggcgc tgtggctgcg gtggccgcgg ctttgtggac gccgcgcagg
      721 acgacatgaa gatgctggtc tgcgctcagt gtcatgtgga atactacttc gcggagccgg
      781 gcaacggtct cacattcccc cgggctcagg gaacgactcc tgcggcggct gtcacggcga
      841 cacagatgcc gacttggcag gactgcgcgc cggcttgatc gaactgcagg caggcatcga
      901 tcagcagcgg acaggtgtgg gcctgctcct ggcagggctg tacggggata tcgacgctgc
      961 cggggcatca ggccggcttg accgcctgcg cgaatcgccc caggccgagc ccgggcctat
     1021 ctcgagagga cgtgccgcag ggtgcagttc agatgggact aggcgttctc cgagaactcc
     1081 actggattcc accaccccga ccttatccgg gccctgaccg gcatcctgcg actgctcatc
     1141 tacagatctg ctacagacgg cagcaggccc cgaactactc atatgccttc cgccacagct
     1201 cgcgggccca ttctaccaag tggtcgattc gctcggacag cgactcctcg gtcagtcggg
     1261 cgccgtctgc cgtagtgtca ctatcaggtg tagctgccga cacagacagg gatccgaacc
     1321 ttacgatgtc ggcctgcagc tcggcgaagg tctgcaggaa gttggggtca agcttctttc
     1381 cgccggtgcc caaggcccat atgagcgagg tcatttccgt tcggtccaga tccttgagta
     1441 gcttcactgc cgcgaccgag ctactatcga tgttcatctc agccaatcga cgatgccatt
     1501 gggcctcgag ttcatccagg ggaacgcctg tgtagcgctc aaacgtgccg aacagctcct
     1561 tgtgggcgga tccgatgtct gctgcttgcc cgatcggccc tgggttttca gggcagtgcc
     1621 tgaagatgtc agtcagggtc ggtctgccgt actcttcgtc tatgaaaccc agcagggatg
     1681 tgggtaccga gtagagcatc cattgattcc aggattgcct gctatgcacc aaggtgagaa
     1741 cctggcggat aggagggaga atgcctgctt cgagggcgct tgctgcggta atgtgcggcg
     1801 ggagcaccca ttgatcctcc tgagcgtcat cagcgtcatc agcgtcatct gccgtggcct
     1861 cccgcagttc cccggcccgc ttcaggccgt cgcgtcgcgc gtactctgga gccagcgagg
     1921 cgaatccttc agcaagagcc ggatacgttg cgcgcgcgtg cagcgcgtga acaaggtgcg
     1981 agtactcgtg taccatgagg aacgagtatc tcctgacctc attcggcgtt tgggggactg
     2041 ctccgttcac gtcggtcacc ctgcaggtcg ccagcgcgaa tgcccccgtc ggggggggtc
     2101 accgacccgg gtttgtcgac ggcgttcagc cacagatgca ccacgggatg gctgaggcga
     2161 ctgccgaaga aatcctcagc atagcgcagc gattctgtgg cggtgtgcag cagaagttgt
     2221 tgtgtctcct cggaagggca gatgtcgtca gggatcagta gtatgacccg gtcattgcca
     2281 atgctcgtgt agcgagtgac ttggtcgacg gtgcgctgcg ccaccgcagc cgatgctgcg
     2341 gcgcccgccc cccacagcat cagcaccatt gcgaccgcgg ccaaacgggc gattccgaac
     2401 ctcctgatag ccctggccaa tatgatcgcc ttacttctta tagagtcttc ctgataccca
     2461 gaaacgtata ccttaccacc tgggttcatc cttatttgcc tccgcaaagg aaacgcacgc
     2521 gtgttcctgt gatcacgtgc gcgtgccttc tgcggcagtc gctcagcaac aagtgtgatc
     2581 gcggtcacaa agtgcatttg tcctccatag cgccacggcg ttcgctgcga atttcccttc
     2641 cggtttatct gtgaaaacat tggaatagtt tctgacacgc gcatcgaaag acggaagtaa
     2701 attttccgaa gttgcaggat tcgcttcagg aggaggcgaa tctaccaaca tcggcgatca
     2761 acgccaccag gcggactcga gcctgggcga acatacgaat gacaaggagg gtttctaatg
     2821 aggcgtctcg ggttggctgt gctcgtatgt ctagtactgg ccgcctcggt cacggccgcg
     2881 cccttcgttg aaggagagga tctcactcct gcaacgaagc ctgtccgtgg aggcacgctt
     2941 tatctcgcac tgacagcttc tccacaatcg ttcctgttct atggttcgct tgataacaac
     3001 gcctacacag tcattggtca gacgatgacg ggtctggtcg aattgcaccc ggtcaccaat
     3061 gctatccggc cggggctggc cgaatcgtgg gagacgtccc ctgacggcaa agaggtcact
     3121 ttccatctca gggacgtgaa gtggtcggac ggcgtgccgt tcacggctga cgacgtgata
     3181 ttcacgttcg agaacttcat catgaacaag gttgccaagg gcaactccgt cgacaggttc
     3241 acgatcctcg atacgcgtga cggccagaag aaagaggtca aatgggttaa ggtcaacgac
     3301 aagaccgtga aggcggtcct gccatcgccg tttggaccgt tctacatgaa cctttcgcat
     3361 gcgtacatat accccaggca caagctggag agcaagatcg acaagaacag gcccgactca
     3421 gtcaatgagc tgtggcttac taatgtctcg ccaaaggaga tcgtggccaa cggtccgtac
     3481 aggatcgcaa gctacgtgat ggatcagaaa gtcgtcctgg agagaaaccc gcactactgg
     3541 agggtcgaca ggttcggcaa ccagctgcct tacttcgaca agcttgagta tctgatcgtg
     3601 aaggatgccg aggtgcggtt ggccaagttc atcgcgggcg agatcgacta catggcggtc
     3661 agcgccgccg acttcccgac gctgaagcag aaggaattgg cgggcggccc gtttacgatc
     3721 ttcaaggccc agcccaccca gccaacgccg agcccagtgc atatcgcatt caactacgat
     3781 gtcgctaatg agcagcttaa ggggatcttc aggaacaccg agttccgaca ggcaatggaa
     3841 ttcctgctcg acagggagcg cataatcgac gaagtctaca acggactggc aattccaggc
     3901 gcgggcctag tgcttccggc caacaaggcc ttctacaacc cgaagatcga gaagatcatg
     3961 aggccgtacg acccggccaa ggccaaggcg atgctcgata agctcgggct gaaggacaag
     4021 aacggcgacg ggatcaggga gttcgcaaat ggcaagaacg tggagttcac cctgaccgtg
     4081 cagagcacgc cgcaggacta tcaggacgta gcgctggtgt tcaaggagga tctggagaag
     4141 gtcgggatca aggtcaactt gcagatcctc gacagcacgc tcgtggggaa tatgtttggc
     4201 gccggcaact tcgaagtcgg aatcagggcg ttcggcaacc agcccgacct cgagttgagg
     4261 aaggcgatat ggcagccggg cacgcagctg tattactggc actactctgc gctcgaccgc
     4321 gacaagctgg ccgcgattcc cgcgaacatg ctcgactggg agaagagggt ctacgagctg
     4381 ttcgacctgg gctcgatcac gaccgatccg gccaagagga aggccatcta cgacgaagtg
     4441 caggagatct acacggatgt tgttcctgtg atcttcgtgt gcaagggcat gaacctgtcc
     4501 ggcgcgaaca agtcgcttgg caatgtgacg caggacaagg aaggtatagt ctacttcgct
     4561 acctacacgg ccttcaggaa gtaacggtgc aattgggggg tggagggggg aggcgctgcg
     4621 cttccccccg tcttcaccag cctgcgggtg aacgtgtgag ttgcgccagg gagtgaacga
     4681 atcgatgtgg gcgttcacag caagacggct gttgatcatg gtccccatga tgatcgtgat
     4741 atcagtgatc tgcttcggga tcaccgaact ccagccgggc gatttcgtca gccagtacct
     4801 cgacaaccca aggatatccc ctgagcagat caggctgcta agggagaggc tcggcctcga
     4861 taagcccgcc tatgtcaggt acataatgtg gatcaagaac atagtcacca aatgggactt
     4921 tgggtactca ttcgcatatg agcgtcccgt gggcgaactg atctttgagc ggatgggctg
     4981 gacggtgttc atcgctctgg caaccatcgt gtttcagtgg atcctggctg ttcccatggg
     5041 catatactca gctctgcatc cgtattcacc cgccgactat gccatgacgg ttgtcgggtt
     5101 cataggtatc tccatccccg acttcttctt tgcactcata ctaatgtact tcatgctccg
     5161 gatgggctcg acctccatcg gtgggctctt ctccaccagg ttcatcggcg ctccttggag
     5221 tttcgccaag ctcatagacc tactcaagca tctatggatg ccgcttgtag ttgtgggcat
     5281 tagcggggtt gcggggctga tgagggtgat gagaggcaac atgctcgata tcgtggggtc
     5341 gcccttcatc acatccctca gagcacatgg tctcgacgaa aagacggtga ggcgacatgc
     5401 catcaagaac gccctcaacc ccatggtgag tatcgccggg gccgaactgc ccaacatatt
     5461 cagcgggacg atcatcacgg ctattgtgct gaaccttccg acgatggggc cgttcttcta
     5521 caacgcgctg ctcaaccacg atcaatacct ggttatgtcc ttcttgatgt tcatagcgct
     5581 gatcacacag atcgggaacc tgcttgcaga catagcactg gcctttctcg atccgagaat
     5641 ccgaatgagc taggagtggc cc
//
LOCUS       DAOTAC010000163         5600 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_12149_length_5600_cov_4.876285, whole genome shotgun sequence.
ACCESSION   DAOTAC010000163 DAOTAC010000000
VERSION     DAOTAC010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5600
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12149_length_5600_cov_4.876285"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="PLM74_07695"
     CDS             <1..563
                     /locus_tag="PLM74_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON42994.1"
                     /translation="MAFAVVLALIWEYAGLDARTASTYWIVRSTAVGGPHDRVGMVNT
                     ATWTAETAGDLVSDTPCPGADAPCPGGESDAKALFDCAKLPAISPEECELLERAAREG
                     ADLGEVDRALAVLMKLLIHPTLVRDAALRRDLVDHQRRLHARHAVLSMQASGQGGDIG
                     QSAARAQVQARLARLYSSLREEYSDD"
     gene            556..1041
                     /locus_tag="PLM74_07700"
     CDS             556..1041
                     /locus_tag="PLM74_07700"
                     /inference="COORDINATES: protein motif:HMM:NF017088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HON42995.1"
                     /translation="MIRAYELMGRPVITVDEGEIVGETAGVIIDTASADVAALIVNVA
                     SRFTPPKAVPYALVQGIGDDAVIIENASSLADFGAIPEILDIGRPDIEIRGVRVITRT
                     GRLLGTVADLMIDLDAGKVVAYQIQGGADTALLVRAEHVITVGRQALITTAEACAHTA
                     E"
     gene            1057..2226
                     /locus_tag="PLM74_07705"
     CDS             1057..2226
                     /locus_tag="PLM74_07705"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HON42996.1"
                     /translation="MYRILHRACIPVSAGISRGCVFVLLVAVIANAVSLGALAFVQIA
                     VFVNDREVYLPFGARVAQALAAAGVELTAGDLIDTDGAVLEVGGGNPAQLTLNGARCD
                     IADRVAPGDRIKAIPGTPIIESIVVDTTETAQPLETRGSGAFVKVQSPGAPGKVTVKR
                     GLITGKVFESAKSHEPTAMVVARFQYAVAKKLIILSFDDGPHPVYTPQILDVLRNEGV
                     HAVFFVVGAQVAAYPGTLKRIHAEGHEIGNHTYSHTLTESALECDILSEINNTSRLVE
                     ELTGNPTRFVRPVGGALSTSFVHAAESTGHTIVLWNVDTQDWRSARGSGRSDQIRLSV
                     SDIPLANGSVILMHDGGGYRGATVQALKAIIPALREQGYQFCTLSELWHLLGLDV"
     regulatory      2360..2483
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            2681..3103
                     /locus_tag="PLM74_07710"
     CDS             2681..3103
                     /locus_tag="PLM74_07710"
                     /inference="COORDINATES: protein motif:HMM:NF038093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HON42997.1"
                     /translation="MSLSSHCYTIVTNNPEVAASWPQAAEWVDGEARSVLIRVRGLVH
                     LGHRLLTHPLAGSVKPNESPYRSVIVSQHPDATIDFDSLRIIEGALAVVDRLGEPRFQ
                     TIPPHVDADFRLIDRTLMESAIESLRAQYGCVAHEICT"
     gene            3119..4351
                     /gene="trxB"
                     /locus_tag="PLM74_07715"
     CDS             3119..4351
                     /gene="trxB"
                     /locus_tag="PLM74_07715"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HON42998.1"
                     /translation="MAVRECDVLIIGAGPAGLAAALYASRAKLRTLVLEKGTPGGQVR
                     TTWSMENYPGFARGMTGPELMDRFADHAKEFGAEIVRGDVVAVDLGGDRKVVKTHGGD
                     EYRARAVIVAPGAEPRTLGLPGERELRGKGVSYCATCDADFFEDLDIVVIGNGDAAVE
                     EAMYLTKFVNSATMIVVHDEGILDATAIIRDRAFKNPKMRWVWNSVVEEIRGDGIVES
                     VVTRNIKTNERTELETNGVFVFVGTVPKTDFLRGHVELDKRGYVVANELMETSADGVY
                     CAGDARVKYLRQVITAAADGAIAAVAAEKYIHEEEGFREMVLDPEQPVMVAFWSPTSQ
                     ASLDFLPAVERVAEERRGKLNLVKIDIYKNQRVAGRYGVAEAPTMAFFRGGQLVEKVC
                     WPTNESELAAVADKVIGE"
     gene            4388..4708
                     /locus_tag="PLM74_07720"
     CDS             4388..4708
                     /locus_tag="PLM74_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HON42999.1"
                     /translation="MFEVNKENFEAEVLGAAGPVLVDFWSTKCEPCMALVPEVEALAA
                     KYGDRIKFCKLNVLENRRLSIGQKVLGLPTIAIYKNGEKVAELTKDDATVANIEAMIK
                     EHGR"
     gene            4714..>5600
                     /locus_tag="PLM74_07725"
     CDS             4714..>5600
                     /locus_tag="PLM74_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HON43000.1"
                     /translation="MRLELGKIRIKDVQFGPETCVRDGVLYVDRAEVERIVREDERIA
                     GASVELARPGEEIRIIPVKDVIEPRVKVAGPGGVFPGMVSKVDTVGEGRTHVLAGAAV
                     VTTGKIVGFQEGIIDMTGPGAEYTPFSRLNNVVLVCEPVAGLPQHEHEAAIRMAGLKV
                     AAYLGEAGRDVEPDQVDVYECLPLLEQAAAYPGLPKVVYVYMLQSQGLLHDTYVYGVD
                     AKRILPTLIYPTEVMDGAIISGNCVSACDKNTTYHHQNSPVVHDLLARHGRDFNLIGV
                     VITNENVTLADKERSSNYTA"
BASE COUNT         1160 a         1507 c         1836 g         1097 t
ORIGIN      
        1 ccatggcatt cgcggttgtc cttgcgctca tatgggaata tgcaggcctc gacgctcgta
       61 cggcatctac ttattggatc gtgcgatcga ccgcggtcgg cgggccgcat gatcgggtag
      121 gcatggtcaa caccgcgacc tggaccgccg agacggccgg cgacctggtt tccgacacgc
      181 catgtccggg tgccgacgca ccatgcccgg gtggcgagtc agatgcaaag gcgctgttcg
      241 actgtgcaaa gctgccggcg atcagccccg aggaatgtga gctgttggaa agagctgcac
      301 gagaaggggc ggaccttggt gaagtggaca gggcgctcgc ggtactgatg aagctcttga
      361 tccatccgac tttggttcgc gatgctgctc tgcggcggga tttagtcgat caccagcgac
      421 ggttgcacgc gaggcatgcc gtcttgagca tgcaggcgtc cgggcaggga ggcgacattg
      481 gacagtctgc agctcgggcg caggtccagg ccaggctggc acggctctac tcttcattga
      541 gggaggaata ttctgatgat tagggcatat gagcttatgg ggcggccagt gataacagtc
      601 gacgagggcg aaatcgttgg cgagaccgcc ggagtaatca tcgatactgc atcagctgat
      661 gttgctgcgc tgatagtgaa cgtagctagc agattcactc cgcccaaggc ggtgccgtac
      721 gcgctggtac agggcattgg agatgacgcc gtcataattg agaacgcgtc gtcgttggca
      781 gatttcgggg cgatccctga gatcctggac ataggacgcc ctgacatcga gatacgtgga
      841 gttagggtaa taacgcgtac cggacgtctg ctcgggactg tggcagatct catgatcgat
      901 ctggatgctg gcaaggtagt tgcctatcag atacagggcg gcgcagatac tgctctgctg
      961 gtccgggctg agcatgtgat taccgtgggc agacaagcgc tgataaccac cgcggaggcg
     1021 tgtgcgcata cggcagagtg aggggaacgg attcgagtgt atcgcatatt gcatcgcgcc
     1081 tgcattccgg tttctgccgg gatttcacgg ggatgcgtat tcgtactcct cgttgctgtc
     1141 attgcaaatg cggtgtcgct cggagctctc gcatttgtgc agattgcggt gtttgtgaat
     1201 gaccgggaag tgtatttgcc gttcggcgcc cgggtcgcgc aggcactagc cgcggcagga
     1261 gtcgagctga cggccggcga tctgatcgac accgacgggg cggttctcga ggtcggtgga
     1321 gggaatccgg cccagcttac gctgaatgga gcgcgatgcg acattgccga ccgggtcgcg
     1381 cctggtgacc gcatcaaagc gataccgggc acgccgatca tcgaaagcat tgtggtagac
     1441 actaccgaga ccgcacaacc tcttgagact cgcgggtcgg gcgcgtttgt gaaggtccag
     1501 tcgccgggag cgcctggaaa ggtcacagtc aagcgaggcc tgattaccgg caaagtcttt
     1561 gaatccgcaa aatcgcatga accgaccgca atggtcgttg cgagattcca gtacgcagtc
     1621 gcgaagaaac tgatcatcct gtccttcgat gatggtcctc accccgtgta taccccgcag
     1681 atcctggatg tgctgcgcaa cgagggtgtg cacgctgtgt ttttcgtggt gggagcgcag
     1741 gtcgccgcgt acccgggcac tctcaagcgg atccatgccg aaggccacga aataggtaac
     1801 catacatact cacacaccct caccgaatcg gccctcgaat gcgacatact gtccgaaatc
     1861 aataatactt cgcgtctagt ggaggagctg accggaaacc cgactcggtt cgtaaggcca
     1921 gtgggcggcg ccctcagcac gtcatttgtg catgccgcag aatcgactgg acacaccatt
     1981 gtcttgtgga acgtggatac ccaggactgg cggtcggccc ggggttcggg acgatcggac
     2041 cagatcaggc tgagtgtttc ggacattccc ctggccaacg ggtcagtgat cctcatgcac
     2101 gacggcggag gataccgcgg cgccaccgtg caggctttga aggccataat ccccgccctg
     2161 cgcgagcagg gctaccagtt ctgcactctc tccgaactct ggcatctcct tgggctagac
     2221 gtctagagct acacagttga cagaggcggg cgattccctg tatggcctaa ccaaaaagtg
     2281 cactcgaaat cttgcttgac atagacattg tgatagaata cactaataat cgatagaacc
     2341 tggacatatt gtcagctgga tgaaggtttc gggagaggtc tccttgggat ggaaatcacc
     2401 aagtgggcgc cgaaggagca atggtgctgc gagttaccgc ggtggcgctg caaactctca
     2461 ggcaaaagga tcgaaactgg acagcgtctc tggaaagctt ccaacggaca ccgaaggagt
     2521 aaagctctca ggttccccag acagagaagc gtgcggcgga tatgtattgc cgtatcgttt
     2581 tctctgtttt ttgtttggcc cggtatcaat ggctgccccg tgtgtttgtg gccggaaggc
     2641 ttaaactaag ggcgattatg ccgcgaatgg ttggggggcg atgtccctgt cgtcgcattg
     2701 ctacaccatc gttaccaaca acccagaggt cgctgccagc tggccccagg ctgcggaatg
     2761 ggtggacggc gaagcgcgaa gcgtgctcat tcgagtgcgc gggcttgtcc acctcggcca
     2821 ccgtctgctc actcatccgc tggcggggag cgtgaaaccc aatgaaagtc cttaccgatc
     2881 cgtgatcgtc tcacaacacc cagatgccac aatcgacttt gattcgctgc gcattatcga
     2941 gggcgccctt gccgtagtcg acaggctggg ggagccccgt ttccaaacga ttcctccgca
     3001 tgtagacgcg gattttcggc tgatcgaccg cacgctcatg gagagcgcaa tcgagagcct
     3061 gcgcgcgcag tatgggtgcg ttgcgcatga gatctgcact tgaagggagc actgagcaat
     3121 ggctgttagg gaatgcgacg tactcatcat aggcgccggc cctgcgggtc tcgccgcggc
     3181 cttgtacgca tcgcgcgcga aacttcgcac tctggtgctg gaaaagggga ctccgggagg
     3241 acaggtgcgc actacctggt cgatggagaa ctaccccggg tttgcccgcg ggatgaccgg
     3301 cccggaactg atggaccgtt tcgccgacca cgcgaaggag ttcggagcgg agatcgttcg
     3361 gggcgatgtg gtggcagtgg acttgggcgg cgaccgcaag gttgtgaaga cgcacggggg
     3421 cgatgaatac cgagccaggg cggtcatcgt cgcgccgggc gccgaacctc gcactctggg
     3481 cctgccgggc gagagggagt tgcgcggcaa gggagtctct tactgcgcta catgtgatgc
     3541 cgacttcttc gaggatctcg acatagtagt tattggtaac ggcgacgctg cggtcgagga
     3601 agccatgtac ctcacgaagt tcgtcaactc ggcaacaatg atcgttgtac acgatgaggg
     3661 catcctggac gcgactgcga tcatccgcga ccgcgcgttc aaaaacccca agatgagatg
     3721 ggtctggaac tcagtcgtcg aagagatacg aggcgacggg atcgttgaat ccgtcgtgac
     3781 ccggaacatc aagaccaacg agcgaactga gctcgagacc aatggggtct tcgtattcgt
     3841 gggcactgtg cctaagactg acttccttcg cggccatgtt gagctagaca agcgcgggta
     3901 tgtagtggct aatgagctca tggagacatc ggcggatggc gtgtactgcg ccggcgacgc
     3961 ccgcgtcaag tacctgcgcc aggtcattac tgcggccgcc gacggagcta tcgcggcggt
     4021 tgcggcggag aagtatatcc acgaggaaga gggcttccgc gagatggttc tggatcccga
     4081 gcaaccggtg atggtggcct tctggagccc caccagccag gcttcgctgg acttccttcc
     4141 cgcggtggag cgtgttgccg aggagcgcag gggcaagctg aacctggtca agatagatat
     4201 ctacaagaac cagcgtgtgg ccggcagata cggtgttgcc gaagcgccca ccatggcgtt
     4261 cttcagaggc gggcagctgg tggagaaggt gtgctggcct acgaatgagt cggagcttgc
     4321 cgccgtggcc gataaggtta tcggcgagtg aggttgttcc aaggtacggc gaaggaggtg
     4381 aactgagatg ttcgaggtga ataaggagaa cttcgaagcc gaggtgctcg gggccgccgg
     4441 cccggtgcta gtggactttt ggagcactaa gtgcgagcca tgtatggccc tagtgcctga
     4501 ggtggaggcg ctcgccgcaa aatacggcga ccggatcaag ttctgcaagc tcaatgtgct
     4561 ggagaacagg cgcctctcca ttggccagaa ggtgctgggt cttcccacca tagccatcta
     4621 caagaatggt gagaaggtgg cggaactgac caaggatgac gcgaccgtcg ccaacataga
     4681 ggctatgatc aaggagcacg ggaggtgaat gtaatgcggt tggaacttgg taagattcgc
     4741 atcaaggacg ttcagtttgg gccggagact tgcgtgcgcg acggcgtgct ctacgtggat
     4801 cgggcggagg ttgagagaat cgttcgcgag gacgagagga tcgccggcgc cagtgtggag
     4861 cttgctcgcc ccggcgagga gatccggata atcccggtca aggacgtcat cgaaccgcgc
     4921 gtcaaggtgg ccggcccggg aggggtcttc ccggggatgg tcagcaaagt cgacaccgtg
     4981 ggcgaggggc gcactcacgt gcttgccggc gcggcggtcg tcaccaccgg caagatcgtc
     5041 ggattccagg aagggatcat cgacatgacc gggccgggag cggagtatac cccgttttcc
     5101 aggctgaaca acgtggtgct ggtgtgcgag cctgttgccg ggctgcctca gcacgagcat
     5161 gaggcggcga tcaggatggc cggcctcaag gtggcggcgt acctgggcga ggccggacgc
     5221 gatgtggagc ccgatcaggt cgatgtgtat gaatgcctgc cactgcttga gcaggcggcg
     5281 gcgtatccgg gcctgcccaa ggtggtctac gtgtacatgc tgcagagcca gggactgttg
     5341 catgacacct acgtctacgg agtcgacgca aagaggattc tgcctaccct catatacccc
     5401 accgaggtta tggacggggc catcatctcc ggcaactgcg tgtcggcgtg cgacaagaac
     5461 accacctacc accatcagaa ctcgccggtc gtccacgacc tgctggcgcg gcacgggcgc
     5521 gacttcaacc tcatcggcgt agtgatcacc aacgagaacg tgacgcttgc cgacaaggag
     5581 aggtcgtcta actacaccgc
//
LOCUS       DAOTAC010000164         5582 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_12204_length_5582_cov_5.155962, whole genome shotgun sequence.
ACCESSION   DAOTAC010000164 DAOTAC010000000
VERSION     DAOTAC010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5582
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12204_length_5582_cov_5.155962"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="PLM74_07730"
     CDS             complement(<1..293)
                     /locus_tag="PLM74_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HON43001.1"
                     /translation="MSMMEDWSTDWCARCPEVAEAVETLCAAFVSGGKLLTCGCGGSA
                     ADASHIAGEMVKSFEIARPLPRTLVDEVDRVVGTSGEDGARRAIRAANASGRSL"
     gene            complement(302..1399)
                     /locus_tag="PLM74_07735"
     CDS             complement(302..1399)
                     /locus_tag="PLM74_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013738938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON43002.1"
                     /translation="MGVTLKNVTKRFRSPDGEISAVVAVDNVSLDIRDGEMVTLLGPS
                     GCGKTTTLRIIAGFELPDAGRVLISGEDVTDRPPNKRDTAMVFQSYAIFPHLSVFENV
                     AFGLRLRKAAQAEVDNRVSKMLDLVGLSDMARRSPDQLSGGQQQRVALARAIVNEPVV
                     LLFDEPLSNLDAKLREQMRLEIRRIQQTLGITSVYVTHDQAEAMSLSDRIVIMNEGSI
                     AQTGTPSEIYNRPVNRFVAEFVGRANFIDATVAGGAGGGMVIDVHGHRAEIPAASCRY
                     PAGTRVELVVRPETVKLFRSDCGCASPVCFAGRVTRVVYMGSVAEYDVDVEGMSLLAV
                     VAGPAEHGLFSVGEEVQVGFAVNVAHPLAVR"
     gene            complement(1414..3093)
                     /locus_tag="PLM74_07740"
     CDS             complement(1414..3093)
                     /locus_tag="PLM74_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HON43003.1"
                     /translation="MRVSFRLWRQMVRLSKEPTLLLSIIAIFALLAVFIVYPIVMVAV
                     RSLSSTDAPGLGIYSRLMLSPYVRRSLYNSLVMGAASAVISCAVGFLFAYTVTRTDVP
                     LKGLLNFMAVVPIVSPPFLGAVSVLLLFGANGLITSKLLGIYNYPIYGFKGLLFAQVV
                     TFFPVAYLTLKGVLESISPTLEDAAMDLGASRWEVFSKITLPLALPGIASALLVIFIE
                     SLADFGNPLILAGSDFPTLSVQAYLQITGMYDLPSGAALSVVLLIPSITAFVVQKYWV
                     DRRKYVTVTGKPTSTAIKSVSRPVKWVLFGACMAIVGVVILFYGVIVAGSFARLWGID
                     PSFSLKNYQYVWGVGSEAIRDTLTVAALSTPISGLLGMAIAFLVVRKSFPGKRAMDFI
                     SMLSFALPGTVVGIGYILAFNQKPILLTGTLAILVLNFVFRYFPVGIQAGVATLRQVD
                     PSIEEAAANLGADSATTFRKITLPLMAPAFFSALVFAFVRAMTAISAAIFLVSADWNL
                     MTVQILNQVNSGRLGAAAAFSVILVGLIVVAMALIRLVVDKALGIKYQARF"
     gene            complement(3209..4219)
                     /locus_tag="PLM74_07745"
     CDS             complement(3209..4219)
                     /locus_tag="PLM74_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009451670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HON43004.1"
                     /translation="MKRLSAALVILALTIPLMAGAVQAAPKAITVYSSVDEQNAKKIL
                     DAFTKATGIPYKMVFLSSGPAIARIEAEKGNPQADVWMGAPSENHVTVKNRGLTQPYV
                     SPNASALKPQFRDAEGYWTCFYMNPMGVGVRTDFLSRVKASAPRTWADLLRPEFKGQI
                     QYPSPQTSGTGYNMVAGFVKEWGEAKAFDYLKKLHANVQIYTQSGTAPSQAIAIGQAA
                     IGIQFTPAILQAIDEGYPIQLVFPSDGVTYEAPAVSILKGAGNLEGAKALVDWLISIE
                     GQTVIAQSKTYFYPIHPQAKLAPGMPAFSEINTVEVDTAWSASQKSRLVEKWIAEVLQ
                     GK"
     gene            4582..>5582
                     /locus_tag="PLM74_07750"
     CDS             4582..>5582
                     /locus_tag="PLM74_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43005.1"
                     /translation="MRATTGAGAPVFGLDSRRFKMIIFDWDGTAVTSRAHPADNILWR
                     SETLLGRGVWLAAVTGTNFANLSGQFAGRLSPSVRQRTLFCTNRGSEVYGFSADGQTP
                     LRLHARVAAATEDEAMDRAARRIQHELREQHGLETRIIYERMNRRKLDLIPLPQWADP
                     LKEQLPALHRAVEMRLASCGVSGGLSEVVALTRVICADEGIDARITTDVKHVELGLTD
                     KSDSVRYLIEQVAEMHAIDTSEILILGDEFGPIGGVEGSDYKLLIPAVRDSLCISVGS
                     EPCGVPAGVAHLGGGADTFAAILEAQMRAWRNESPAPSTALSFPLSFCPPTDPWQLR"
BASE COUNT         1202 a         1752 c         1541 g         1087 t
ORIGIN      
        1 aggctccgcc cactcgcgtt cgcagccctg atcgcgcgtc tcgcgccatc ctcgccgctt
       61 gtgccgacaa ctctgtccac ttcgtcaacc agtgtacgtg gcaacggacg agcgatctcg
      121 aacgacttca ccatctcgcc ggcaatgtgc gacgcgtcgg cagcgcttcc cccacatcca
      181 catgtcagca acttgccgcc tgaaacgaaa gcggcacaca acgtctcgac cgcttcagcc
      241 acttcggggc agcgggcaca ccagtctgta ctccagtctt ccatcatgct catgcggatc
      301 cctacctaac agcgagcgga tgagctacgt tcacagcgaa gcccacctga acctcttcgc
      361 ccacgctgaa cagcccatgc tctgccgggc cggccaccac agcaagcagc gacatccctt
      421 cgacatcgac gtcgtactca gctaccgatc ccatgtatac cacccgtgta accctcccgg
      481 cgaagcacac tggggaggcg cacccgcaat cggacctgaa gagcttcacg gtctccggtc
      541 gcaccaccag ttcaacacgg gttccggctg gataccggca gctggcggcc gggatctccg
      601 ctctgtgtcc atgcacatcg atcaccatgc ctcctccggc gcccccagct accgttgcgt
      661 ctatgaagtt ggccctgccc acaaactcgg ccacgaaccg gttgactggc ctattataga
      721 tttccgaagg cgtgcccgtc tgggcaatgg aaccctcatt cattatcacg atcctgtcgg
      781 ataagctcat cgcctccgcc tgatcatgag tcacatacac gctggtaatg cccaacgtct
      841 gctgaatgcg tcggatttcc agtctcatct gctcgcgcag cttcgcatcg aggttcgaca
      901 gcggttcgtc gaagagcagc accacgggct cgttcactat cgccctggcc agggctaccc
      961 tctgctgctg cccccctgat agctggtcgg gggagcgcct ggccatgtcg gaaaggccca
     1021 caaggtcaag catcttggag acacgattat ccacctcggc ctgcgccgcc ttgcggagcc
     1081 tgagcccgaa tgctacgttc tcaaacaccg aaaggtgcgg gaatattgcg tagctttgga
     1141 acaccatcgc agtatcacgc ttgttgggcg gtctgtctgt cacatcctca cctgaaatca
     1201 gcacccgccc agcatcgggc agttcgaagc ctgcgatgat acgaagggtt gtggtcttgc
     1261 cgcagcccga agggcccaac aaggtaacca tctcgccgtc tcgtatgtcg aggctcacat
     1321 tgtcaacagc cactaccgcg ctgatctccc cgtcagggct ccgaaacctc ttcgttacat
     1381 tcttgagtgt gacacccatc tgcatacacc gccctaaaac ctagcctgat atttgatgcc
     1441 cagagccttg tcgacaacca gcctgatcag cgccatcgcc accacgatca agccgacaag
     1501 gatcacactg aacgccgcag cggctccgag gcgacccgag ttgacctggt tgagaatctg
     1561 cactgtcatg aggttccagt cggccgatac aaggaagata gccgcactga ttgccgtcat
     1621 cgccctcacg aacgcgaaaa ccagagccga gaagaatgcc ggggccatga ggggcagggt
     1681 gatcttgcgg aacgtagtcg cactatcggc gccaaggtta gctgcggcct cctcgatcga
     1741 cggatccacc tggcgcagcg tggccactcc cgcttggatc cccacaggga agtaccggaa
     1801 cacgaagttc aacacgagaa tggccaacgt gcccgtgagc aggattggct tttggttgaa
     1861 ggccagaatg tagcctatcc ccactacagt gcctgggagc gcgaaactga gcattgatat
     1921 gaagtccatg gcccgcttgc ccgggaagct cttgcgtact accaaaaacg caatggccat
     1981 gccaagcagc ccggagatag gggtggacag tgcggcaact gtcagagtgt cgcgtatcgc
     2041 ctcgctgcct acgccccaaa cgtactgata attcttgagt gagaacgatg ggtcaatgcc
     2101 ccagagtctt gcaaacgacc ctgccactat gacgccatag aacaggatta ctactccgac
     2161 tatcgccatg caggcaccga acagcaccca tttcacgggc ctgctcacac tcttgatcgc
     2221 cgtgctcgtc ggcttgccgg tgacagtaac gtacttgcgc ctatctaccc agtatttctg
     2281 cactacgaaa gcggtgatgg aagggatcag cagcaccacg gagagcgccg ctccgctcgg
     2341 caggtcatac atgccggtta tctgcaaata cgcctgcact gacagggtcg ggaaatcgct
     2401 gcccgcgagg atcagcgggt taccgaaatc cgcgagggac tcgatgaaga tcaccaggag
     2461 agcactggcg attcccggca aagctagagg cagagtgatc ttgctgaaca cttcccagcg
     2521 cgatgccccg agatccatcg cagcgtcttc cagggtaggg ctgatggact ccagcacccc
     2581 tttgagcgtc aggtaggcca cggggaagaa cgtgaccact tgcgcgaaca gcaacccctt
     2641 gaagccatag atggggtagt tatatatgcc gagcagcttc gacgtgatga gtccgttagc
     2701 tccgaaaagc agcaggaccg acactgcccc cagaaagggg ggcgatacta tgggcacaac
     2761 agccatgaag ttcagaagtc ccttgagtgg aacatcagtg cgcgtcacgg tgtacgcgaa
     2821 cagaaacccc acggcgcagc tgatcacagc cgacgccgcc cccatcacaa ggctgttgta
     2881 gagcgacctg cgtacatatg ggctcagcat gagtctcgag tatatgccca gcccgggggc
     2941 atcagtcgat gaaaggctgc gcacagccac catgacgatg ggatacacga tgaacacagc
     3001 tagcagggcg aagatagcga ttatgctaag gagcagggtg ggttccttcg atagccgcac
     3061 catctgccgc cacaaacgaa aagagactct cattggctct cctccgacat gctgagggcg
     3121 gatcgatgta ggcccggcca ccgacgccat ggagcaacca tggggcatag cagccgggcc
     3181 ttctccgccg aaatagcggg ctatctacct acttgccctg cagcacttct gcgatccact
     3241 tctcgactaa gcggctcttc tgcgatgccg accacgcggt atcgacttcc actgtgttga
     3301 tctcactgaa tgcgggcatt cccggcgcca gcttggcctg gggatggatc gggtagaaat
     3361 acgtcttcga ctgggcaatg accgtctggc cctctattga gatcagccaa tcaactaggg
     3421 ccttggcgcc ctcaagattg cctgcaccct tgaggatcga tactgccggc gcctcgtagg
     3481 tcacaccatc actggggaat actagttgga tggggtagcc ctcgtcgatg gcctgcagaa
     3541 tcgcaggggt gaattgaatg ccgatggcag cctgcccgat agctatggcc tgtgaaggag
     3601 cagtgccact ctgtgtgtat atctgcacat tggcatgaag cttcttgaga tagtcaaagg
     3661 ccttagcttc tccccattcc ttcacgaaac cggccaccat gttgtacccg gtgcccgaag
     3721 tctgagggct cggatactgg atctgtccct tgaactctgg gcggagcagg tccgcccagg
     3781 ttctcggagc ggatgctttc actctactga gaaagtcggt acgtaccccc acgcccatag
     3841 gattcatgta gaagcaagtc cagtagccct cagcatccct gaactgcggt ttcagagccg
     3901 aagcgttagg ggagacgtac ggctgagtca gcccgcggtt cttcacagtg acatggttct
     3961 cgctgggagc gcccatccac acgtctgcct gcggattgcc cttctcagcc tcgatgcgcg
     4021 cgatagccgg gcccgacgac aggaatacca tcttgtacgg gatcccggtg gccttggtga
     4081 acgcgtccag gatcttcttc gcgttctgct catcgacact ggaatacacc gtgatcgcct
     4141 tcggtgcggc ctgcactgcc ccagccatca gaggaatggt cagggccaga ataaccagag
     4201 cagccgaaag tctcttcata gcaacatcct cctacactcg tgcagtcatg gcgcaggcga
     4261 cccgttctcc actcagaatc tgggccttcc gggtcctgct tgatcccatg caccacttct
     4321 ttcgtcattc gacacgaccc ctcccattcc ctttaggaat ccatgccggc tccgcatgaa
     4381 cagattccta gtcagggcga cacatgaaca ccgagttgac ccatcgcgct caaagcaact
     4441 gcttaaacgt ttgagaagat tctccccgta gtgaaggaat tgcactatca gtgcagaact
     4501 agttacgtgc tagaattaac ttttacgatt aagccgctgc cgaccgcgtc tgctgggcag
     4561 cgcactcacg gagggctgtt catgagggct actactggag ctggagcgcc agttttcggg
     4621 ctcgattctc gccgcttcaa gatgatcatc ttcgattggg acgggactgc agtcacctct
     4681 cgtgcccatc cggccgacaa cattctgtgg aggtcggaaa cgcttctagg acgaggggtg
     4741 tggcttgcgg ccgtcaccgg taccaacttc gccaacttga gcggccagtt tgcgggcagg
     4801 ctgagtccga gcgttcggca gcggaccctg ttctgcacaa accggggatc tgaagtctac
     4861 ggtttcagcg cagacggcca gaccccgctc aggctgcacg cccgcgtggc ggctgcgacc
     4921 gaggatgaag ccatggatcg agcagcccgc cgaatccaac acgaactgcg tgagcagcat
     4981 ggactggaaa cccggatcat ctacgaacgc atgaaccggc gcaagctcga cctgatcccc
     5041 ctgccccagt gggccgaccc gctcaaggaa cagctgcccg ccctgcacag ggccgtagag
     5101 atgcgactag cgtcctgcgg ggtatcggga ggcctgtccg aggtagtggc cctgactcgc
     5161 gtgatctgcg cggacgaggg catcgatgcc cggattacca ctgatgtgaa gcacgtggaa
     5221 ctcgggctaa ccgacaagtc agattcggta cgctacctga ttgagcaggt agcagagatg
     5281 cacgccatcg acacctcgga gatcctgata ctgggcgatg agttcggtcc aatcggagga
     5341 gtcgagggca gcgactacaa gctgctcatc cctgcagtgc gcgattcgct ctgcatcagc
     5401 gtagggagcg aaccctgcgg agtcccggca ggcgtcgccc acctcggagg gggcgccgac
     5461 accttcgccg ctattctcga agcacagatg cgcgcatgga ggaatgagtc tcccgcgccg
     5521 tcgaccgcgc tgtcattccc tctctcgttc tgcccgccca cggatccgtg gcagctacgt
     5581 ga
//
LOCUS       DAOTAC010000165         5561 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_12261_length_5561_cov_4.943879, whole genome shotgun sequence.
ACCESSION   DAOTAC010000165 DAOTAC010000000
VERSION     DAOTAC010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5561
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12261_length_5561_cov_4.943879"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="PLM74_07755"
     CDS             <1..557
                     /locus_tag="PLM74_07755"
                     /inference="COORDINATES: protein motif:HMM:NF013895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HON43006.1"
                     /translation="MPLDRPYFATSVKELWRSRVVWLMVLFLAESVTGKILQNFSTAM
                     QSVIELTFFIPLLIDCGGNAGSQAASVVIRGLAVRDIRMRDVWSVFARELTLGLALGL
                     SMSGIAFLRAWRVGGNPIIGVIVCTSVLVIVTVATIVGSMLPIAATALHLDPAVMSSP
                     LITTIVDGTGLMIYFSIARALLKL"
     gene            complement(662..1660)
                     /locus_tag="PLM74_07760"
     CDS             complement(662..1660)
                     /locus_tag="PLM74_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HON43007.1"
                     /translation="MNWRPTIGFCLAVTLAIMGAGCALKPPAAVGSGSVVVAAAIKSV
                     PSASSAPELIVLTLSMGGFTLKHEMPVEGGAGHGTIESLAAGNWLIDAVAVDGDGDAI
                     YAGRAKAQVKRDSVIPVSITLRAVEGRLRIQVSAAGIASTVPVHSADIFIYYASSSSA
                     YRSYKDLREQDGYFIVEDTGYPPRSYDFRVVLKADDGSVLYESEYASFGVCPGKFTGI
                     NWSPGLGDIQVNVDFLMAPGAPEDVGVSLDGTQAAVSWTAPPDGLAASYRVYWRYSLF
                     DRYTTSRSQQVDAPATSALVSLSSQQRGSTVHFVVVARDSSGQESLRSSEVAVEYR"
     gene            1883..3871
                     /locus_tag="PLM74_07765"
     CDS             1883..3871
                     /locus_tag="PLM74_07765"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HON43008.1"
                     /translation="MVEQLRQEQEPKRTQTRLPVPVTRMVRSILAAATIMAVSWGVIY
                     LGAARADQEARHNLLMRARTSAALLDPCDIADLTQYALGDDLRSSDTFIRVLDRLRAL
                     YQTSEDTRFVYIVQPVDGQIVSILDAEPEDSPDYSPPGQSMYPATPGEMALFASGTAG
                     VEGPRADPWGTWITAYAPIASDSGDSPVAFLAMDIDASAWPKYALFQWTPVFLTVAFL
                     AALAVFMVVLKYIDESRHARYLAERDYLTGLANRATLIRAIDRAILQARKGGPSALLA
                     IDIDNFKALNHTLTYSMGDEILVAVAGHVKTLVRRTDMVARLAGDEFAVLLTGVARDE
                     ALATAERIRSSLEMLRFEVRGMPVYLTVSTGMIEIGRNTHTEDVAMRADMALAAAKEA
                     GKNRIAFSPTYAAPAPNGESVTYSDTLAIEAALRGDRLLVYLQPIVPVTDTQPQESDY
                     RCFEALVRMIGADNNVVPAGKFVPIAEGLGLIPQIDMWVLDRVIELLSAQPDVSIAMN
                     LSARSIARPSFLEQVEAKVKSAGIGQGRLCFEITETAAMGNVSEVRSWMNRMKALGCV
                     FAVDDFGVGHSAISYLYELPVDIVKIAGNVVQSMRDDSSGKLVVEAINSVSHVLGAKT
                     VAECVEDEECVAVLQDMGIDFGQGYYFARPMPAHDALA"
     gene            complement(3929..4004)
                     /locus_tag="PLM74_07770"
     tRNA            complement(3929..4004)
                     /locus_tag="PLM74_07770"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3968..3970),aa:Glu,seq:ctc)
     gene            complement(4011..4084)
                     /locus_tag="PLM74_07775"
     tRNA            complement(4011..4084)
                     /locus_tag="PLM74_07775"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4050..4052),aa:Gln,seq:ctg)
     gene            complement(4219..4698)
                     /locus_tag="PLM74_07780"
     CDS             complement(4219..4698)
                     /locus_tag="PLM74_07780"
                     /inference="COORDINATES: protein motif:HMM:NF015388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:2001070 - starch binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HON43009.1"
                     /translation="MWQRKYGGDRGDESRERDVDFEFASELTPPGLGSPAKTGRTTGA
                     RSKTTRGGTAKESSLMGGVSVSPMPVTAGERVTVKYNGLLAQAGADTLYLHSGFGSRD
                     WRSVSDIPMTQEKAGTWKAQVDLDPNETSRLNFCFKDRANNWDNNYGLNWSLEIHNG"
     gene            complement(4798..>5561)
                     /locus_tag="PLM74_07785"
     CDS             complement(4798..>5561)
                     /locus_tag="PLM74_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HON43010.1"
                     /translation="APYTCSVSFLSAVARRASQLGVGIHTHISETFREVDGIRSAHGM
                     SPVEYMESSGLFDVPTLAAHCVAVSDDDINILAKYGVRVAHNPGSNMKLASGVAPVEK
                     MLAAGIAVGLGTDGAASNNNLDMVEEMRLAGLLQKVTRLDPTAVPALVCLEMATRGGA
                     TCLGLADCIGSIEVGKRADIVLLDFEAPHLSPSGIADPVSHLVYSASGADVSTVVVDG
                     CVLMDDRRLTTLDEREIMAEVERRAVRLAEEANVK"
BASE COUNT         1145 a         1726 c         1533 g         1157 t
ORIGIN      
        1 ccatgcctct cgaccgaccg tacttcgcga cctcagtcaa ggagctctgg cgcagccggg
       61 tagtctggct gatggtgctg ttcctggccg aatccgttac aggaaagata ctgcagaatt
      121 tcagcacagc tatgcagtcc gtcatcgaac tgacgttctt cataccgctc ctgatagact
      181 gcggcggcaa tgccggttcg caagccgcct cggtggtcat ccgaggtctc gcggtacgcg
      241 acatacgaat gcgggatgtc tggtcggtat tcgcccgcga gcttacgctg ggcctcgctc
      301 taggcctgtc gatgtcgggc atagcattcc tgcgggcctg gagggtcggc ggcaacccaa
      361 ttataggcgt aatcgtgtgc acatccgtgc tcgtgatcgt cacagtggcc acaatcgtcg
      421 gatccatgct gcccatagcg gccacagcac ttcacctgga cccggctgtc atgtcgtcgc
      481 cgctgatcac cacaatagtg gacggcacag gcctcatgat ctacttcagc attgcccgag
      541 ccttgcttaa gttgtaggtc ggcgccttct tcccaggccc aggcgttatt gtgcgattct
      601 catgacgctc cgcccgcgcc catagcgcgt gcggagcgac cttcatgctc taccgtatgg
      661 cttaccggta ctccaccgcg acctcggagc tcctcaggct ttcctgccct gatgagtcgc
      721 gcgctaccac cacgaagtgg actgtcgacc ctcgctgttg ggaggacagc gacacaaggg
      781 cggacgtcgc gggcgcgtcg acctgctgag acctggatgt tgtataccga tcaaacagcg
      841 agtatctcca atacacccta taggacgccg caagcccgtc aggaggagcc gtccatgata
      901 ctgccgcctg tgtgccatct aggctaacgc ccacatcttc tggggctccc ggcgccatca
      961 ggaaatccac atttacctgg atgtctccca accccgggct ccagttgata ccggtgaact
     1021 ttcccgggca caccccgaag ctggcgtatt cgctctcata cagcactgat ccgtcgtctg
     1081 ccttcagcac cacgcggaag tcgtatgaac gcggagggta tccggtgtct tcaacaatga
     1141 aatacccatc ctgctcgcgt aagtccttat aggaacgata cgcggagctg ctactggcat
     1201 aatagatgaa gatgtcggcc gaatgaacag gcacagtgct cgctattccc gcagctgaca
     1261 cctggatcct gagacgacct tccaccgcgc gaagagtgat cgacacaggg atcacactgt
     1321 cccgcttcac ctgggctttg gcccggcccg cgtagattgc gtcgccgtcg ccgtccacgg
     1381 caaccgcgtc tatcagccag ttcccagcgg caagagactc gatagtgcca tgacccgcgc
     1441 ctccttctac aggcatttcg tgtttgaggg tgaatcctcc catactgagc gtcagcacaa
     1501 tcagctccgg cgctgacgac gcacttggaa cgctcttgat ggctgcagcg actaccacag
     1561 atccgctgcc gaccgcagca ggcggcttga gcgcgcaacc cgctcccatg atcgccaacg
     1621 tgacggcaag gcaaaatccg atcgttggcc tccagttcat gttacatcac tccattctat
     1681 ggaatttgcc cattcgctgc cactgattct ccgcctgcag cctctgtcct gccgcctgcc
     1741 accagaatca cccgggtgat taagcgcgtt ctgggcgcag ggattttcac gactaatgcc
     1801 gaatcagctt ttttggatgc accctagcct gccaactggc gatcaacgac gatcgtcatc
     1861 ctctgggcaa ggaggcctgc acgtggtgga acagctacgt caagagcaag agcccaaacg
     1921 cacacaaaca cgtctgcccg ttcctgttac ccgaatggtc cgctcgatac tggcagccgc
     1981 aactataatg gcggtaagct ggggagtcat atatctagga gccgcccgag ctgatcagga
     2041 ggcgcgtcac aacttgctca tgagggctcg aacctcggcc gctctcctgg atccgtgcga
     2101 catagccgac ctcacccaat acgccttggg agacgacttg agatcgagcg atacgttcat
     2161 ccgggtgctc gatagactga gggctcttta ccagacgtct gaagacaccc ggtttgtcta
     2221 catcgtacag cctgttgatg ggcagattgt gtcgatcctc gatgcggaac ccgaggattc
     2281 gcccgattac tcccccccgg gtcaatccat gtatccggcg acccccgggg aaatggcatt
     2341 gttcgcctcc ggtacggccg gggttgaggg cccaagagct gatccttggg gtacctggat
     2401 caccgcctat gccccgatcg ctagcgactc aggcgacagc cctgttgcct tcctggccat
     2461 ggacatcgat gcctctgcct ggcccaaata cgcgctattc cagtggactc cggttttcct
     2521 gaccgtcgcc ttcctggcag cgctcgcagt cttcatggtc gtcttgaagt acatagacga
     2581 atcccgacac gcccgctacc ttgccgagcg cgactaccta accggccttg ctaacagggc
     2641 cacgctcatt agagcgatcg accgggctat cctacaagcg aggaagggcg ggcccagcgc
     2701 cctgctggcc atcgacatcg acaacttcaa ggcgctcaat cacacgctca cctattccat
     2761 gggtgacgag atcctggtag ctgtggctgg tcatgtcaaa acgttggttc gcaggaccga
     2821 tatggtggca aggctagccg gcgacgagtt cgcggtgctt ctcacgggag tcgcgcggga
     2881 tgaagcactg gccactgcag aacggataag gtcatcgctg gagatgctta ggttcgaagt
     2941 aaggggcatg cctgtgtacc ttactgtcag cacaggaatg attgagatag gccgcaatac
     3001 ccacaccgaa gacgtggcca tgcgtgccga catggctcta gctgctgcga aagaggcagg
     3061 caagaatcgg attgccttct caccgacgta cgctgctccg gccccgaatg gtgaatcagt
     3121 gacgtattcc gacacactcg cgattgaagc ggctctccgg ggcgataggc tactcgtgta
     3181 tctgcagccc attgtacctg tcaccgacac ccagccacag gaatccgact accgctgctt
     3241 tgaggcgctc gttcgcatga ttggggccga caacaacgtg gtaccggccg gcaagttcgt
     3301 gcccatcgca gagggcctgg ggctgatacc ccagatcgat atgtgggtgc tggatcgagt
     3361 gatagaattg ctgtccgccc agcccgatgt gagcatcgcc atgaacctgt ccgccagaag
     3421 catcgccagg ccctcgttcc tagagcaggt cgaggcgaaa gtgaagtcgg ccggcatcgg
     3481 ccagggccgg ctctgtttcg aaatcactga gacagcagcc atgggaaacg tgtccgaggt
     3541 gcgatcgtgg atgaaccgaa tgaaggcact aggctgcgtt tttgcagttg atgacttcgg
     3601 cgtagggcat tcggccatct catacctgta tgaactgccc gtggacatag tcaaaatcgc
     3661 aggcaatgtg gtccagtcca tgagggacga ttcctccggc aaactcgtcg tagaagccat
     3721 caactcagtc tcgcacgtgc taggagccaa aaccgtagct gaatgcgtag aagacgaaga
     3781 gtgcgtcgcg gttctgcagg acatggggat cgacttcggc caagggtatt actttgcccg
     3841 cccgatgccg gcacacgatg cgttagcctg agcagaggcg ctagactgag aaaaagaaaa
     3901 caccctgatc gctcagggtg ttcaaacttg gtagccccga cggaattcga atccgtgtct
     3961 ccgccttgag agggcgacgt cctaggccac tagaccacgg ggccatgaat tggctgggga
     4021 ggaaggattc gaaccctcgc aatcagatcc agagtctgac gtcctaccgc tagacgactc
     4081 cccaacgctc aacagcgtcc gttgatgatt atagcaaagg cctgagggtt tgtaaaggca
     4141 gaaggcagga tttcaggcac gtttgtgcgt gcctccatcc tgcctgccgg tgcgaagggt
     4201 ctgccgaaac cgccctacct atccgttgtg tatttccaag ctccagttga ggccgtagtt
     4261 gttgtcccag ttgttggccc tatccttgaa gcagaaattc aggcgggagg tttcattcgg
     4321 atccagatcc acttgtgctt tccaagtgcc cgctttttcc tgcgtcatcg ggatgtcgga
     4381 gacactccgc cagtctctgg acccaaaacc tgaatgcagg tacagggtat ccgcgccggc
     4441 ctgggccagg aggccgttgt actttacggt gacacgctct ccagcggtga ccggcattgg
     4501 cgaaaccgac acccctccca tcagggagct ttccttggca gttccgccac gcgttgtctt
     4561 ggacctagcg ccagtagtgc gcccggtctt ggccggcgag cccagacctg ggggcgtgag
     4621 ttccgaagcg aactcgaaat cgacgtccct ctctcttgac tcgtcgcccc tgtcgccgcc
     4681 atatttccgt tgccacaaca cccggcgaga cctcctttca ggcatattct ctcgccgcag
     4741 cgccgtagtt attagggcaa atacgacctc acaatgattg cgcatcacct attgccttta
     4801 ttttacattc gcttcttcgg ccaaccttac tgccctgcgc tccacctccg ccatgatctc
     4861 acgctcgtca agtgtggtca gtcttctgtc gtccatcagc acgcagccat ctaccacaac
     4921 cgtgctcacg tcggcgcccg aagcagagta caccagatgc gaaaccggat ccgcaattcc
     4981 ggaaggcgat aggtgcggag cctcgaagtc aagcagaacg atatccgccc tcttaccaac
     5041 ctctatagat ccaatgcagt cagcaagccc aaggcacgtg gcgccgcctc tggtggccat
     5101 ctccaggcac acaagtgcag gtacggccgt cggatcaagc cgagtcacct tctgcagcag
     5161 gcctgcgagg cgcatctcct ccaccatgtc aaggttgttg ttgctggccg cgccgtcagt
     5221 gcccaatcca accgcaatgc ctgccgccag catcttctcc acaggcgcaa cgcccgatgc
     5281 cagtttcatg tttgacccgg ggttgtgcgc cacccgcacc ccatacttcg ccaggatgtt
     5341 gatgtcgtcg tccgacaccg cgacgcaatg ggcagccagc gtcggtacgt cgaaaaggcc
     5401 gcttgactcc atgtactcca cagggctcat gccgtgagcg gagcggattc cgtctacttc
     5461 gcggaaggtt tcggaaatat gggtatgtat tccgacgccc agctgactcg cacgccgggc
     5521 caccgcagag agaaaagaaa ctgagcaagt gtacggcgcg t
//
LOCUS       DAOTAC010000166         5552 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_12288_length_5552_cov_5.390941, whole genome shotgun sequence.
ACCESSION   DAOTAC010000166 DAOTAC010000000
VERSION     DAOTAC010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5552
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12288_length_5552_cov_5.390941"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="PLM74_07790"
     CDS             complement(<1..542)
                     /locus_tag="PLM74_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43011.1"
                     /translation="MDVILVAGTPGDVHGLARPLAERVAAVMSVADVWVFVSPSSHAS
                     GREAKVASSYPGVRRAIGPLEFLKYLAVGAAPRGFARSGKGVVIHLGGDLIYSVDLGR
                     RLRYPVFAYSSRAMPGAKSISLFLVEDERVQASLIARGVAAERIKLVGNLMIDGVGAR
                     RGREESRSRLGVEQYERVIML"
     gene            complement(605..1369)
                     /locus_tag="PLM74_07795"
     CDS             complement(605..1369)
                     /locus_tag="PLM74_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HON43012.1"
                     /translation="MLSIRDLRKTYGSRQALTGFSLNVEQGETVAVMGPSGCGKSTAL
                     KSIIRLVQPDSGAIEWCGRSVLGMSCDELRAFRRRTGFVFQRANLVQRLSVLHNVMLP
                     GVMSGEPAARAERAAYEALESVGMAESAGASVTGLSGGEMQRVAIARAIANRPDLVLW
                     DEPTSALDPILVIDILTLIESLSRALGATMVVVTHEAGFAMRAADRVVLVDQGRVVEE
                     GTPYRVFSCPTSILGGKYAACVAYSASAQKEKASGL"
     gene            complement(1348..3687)
                     /locus_tag="PLM74_07800"
     CDS             complement(1348..3687)
                     /locus_tag="PLM74_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43013.1"
                     /translation="MYALLRGGFIRDVVGVLIITVVVGSIAAATVSAAADRFFEDTVS
                     GIVGDYGEYDLIIHVRQEAWHQAARALGQMLRRDFPGARLKQSVTVLSRANFFVSLPH
                     TDEATVTRLTRAATEVPGFAGYTIVLEPKVTVSGVGARVAPIIRPQLAAIEGVRFTLK
                     DGGNIHLVCDSGDSARAVAQTAEGILRRYHLVDVRPESVEVSDGDRQSLIDKVMAGDG
                     ASAVHDVTADGAAQEARELGRALKEMKRFIEAWAPQAVITLKDADVQPEESLIMSATP
                     GRGGSAVGDGDIVVALTEVSDSAARGIVINSDVDGATGELIAYAMDGEMVGPRVGTGR
                     LVLPSRELGNSLQEASAVLGRLGQATGAMLEATRGWEQALDAYETAIDSMIRLQRALE
                     RVGIGDGVGTDPARMRIDPASVRSLAELTSKALEAIRTLETAAEAATMFTSHYDPLLS
                     NLRLWQSRLESFAGKLETVQSAVSGAGDVATLLDEMSGAAAGILSTLQELDAAALGEQ
                     LEAARSDLDSLAGLDVEGISKQLEAVQSRTPDFSGEGAARSIRMIDQFIEEQQESSGK
                     VELLVESDTPQEQLAKVIGKAAGTDAAVYILPPGVVQPGVRSEVRTLLKSVKGTISGL
                     AACAIVLLSLILDHSTVIAGASAMALHGEGTSRRDALAVYGYGSCVGALLLGTIAAVT
                     RATLASMGFPAFVVLGMAMGLVVAWAAPRLSPVNADEVEACIALGMSPSEMMREIVAP
                     AGRPGMLSILNRPRLVFGDTASSGATSKGGASQCSQYAT"
     gene            complement(3777..4442)
                     /locus_tag="PLM74_07805"
     CDS             complement(3777..4442)
                     /locus_tag="PLM74_07805"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HON43014.1"
                     /translation="MRRVLRSGILLFVVAVFALSIPVQAKPVKVTAYQSVVHKGAKGD
                     TVFEGGLKIEYDDIVITADRATVNMDAGIAKLGGKVALVQGDVTIGCDSMDLNIKKKI
                     AAVTGSVRLQKRETQVEKDEAGKPKVSVITLTCTAMEIFTDTQDFNASGNVSMTRDKQ
                     RAQAAHASYKNSEKMLILTGSVFIEGENKESIKCDRAELHTDQTMVAAEGTKMEITFD
                     IEE"
     gene            complement(4462..5151)
                     /locus_tag="PLM74_07810"
     CDS             complement(4462..5151)
                     /locus_tag="PLM74_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43015.1"
                     /translation="MSAKLVKAVSISVAVLLACALIYAFVINRPDPPDADPASERGRG
                     TKQPASRDLLFDGVAVEGREGGERVWMLSAARVETGAGDGRAEMYKITEGAIYRSGEP
                     YLTFSAGRGLYDSKTGQLRLDGGVKVTSGGKDLLATDEVVWDPKQSKVLIPGKTEIST
                     SAGVVTVSQLEVDIEADKMYTAGDVLVSEAEGKGSFQMEGDDIHFSSDGESFEARGRT
                     TIQFELDDSEE"
     gene            complement(5180..>5552)
                     /locus_tag="PLM74_07815"
     CDS             complement(5180..>5552)
                     /locus_tag="PLM74_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43016.1"
                     /translation="GYDIQQALPKSLLGRVVVQPSPEFQLRAGGYYDFARGRMTRVES
                     SADMRPLPDWRIQWVAVYDLVKGGLVRGDIGITRDLHCREIAVTYQYTTRRVWLEFRF
                     KAFPGTPLGFGLGEDGVIFGQ"
BASE COUNT         1125 a         1904 c         1417 g         1106 t
ORIGIN      
        1 agcatgatga cgcgttcata ctgctccact ccgagacgcg aacgagactc ttcccgcccg
       61 cgcctggcgc caacgccatc aatcatcaga tttcccacaa gcttgatacg ttctgcagcc
      121 acgccgcgtg cgatgagtga agcctgaacc ctttcgtctt caaccaggaa tagagagatg
      181 gatttggcgc ccggcatggc cctgctggaa taggcgaaca caggataccg cagtcggcga
      241 ccgaggtcta ccgagtagat caggtcgccc ccaaggtgaa tgaccacgcc cttcccggat
      301 ctcgcgaacc cccggggcgc agctcctacc gcaaggtact tgagaaactc caatgggcct
      361 atggcccttc gcactcctgg gtacgacgag gctactttcg cctctcgtcc gctggcgtgg
      421 gaggacggcg agacgaacac ccacacatcg gcaaccgaca taacagccgc aactcgttcg
      481 gccagcggac gcgcgagacc gtgaacgtcc cccggcgtgc ccgccaccag tatcacatcc
      541 aatgcaactc accgtcccgc cgcgacgcgg caaccgccgc tcatgcccct aaaacttaga
      601 atttctacag accgcttgcc ttctccttct gagcagaagc cgagtacgcc acgcacgctg
      661 catactttcc gccaaggatg gaggttggac acgagaatac gcggtaggga gtgccttcct
      721 caacgactct cccctggtcc accaatacaa ccctatctgc ggcgcgcatg gcaaatcccg
      781 cctcgtgcgt cactaccacc atggtagcgc caagcgctcg cgaaagcgac tcgatgagag
      841 tgagtatgtc aatgacaagt atcgggtcta gagccgacgt cggctcatcc cacaggacta
      901 gatcaggccg gtttgcaatt gctctagcta ttgctaccct ctgcatctct ccgcctgaca
      961 ggcccgtcac ggacgctccg gccgactctg ccatacctac agactccaat gcctcgtagg
     1021 ccgcgcgctc cgctcttgcc gcgggttctc cagacatcac tcccggcaac atcacattat
     1081 ggagcacgct cagacgctgg acgagattcg cacgctgaaa cacgaacccg gtgcgccgcc
     1141 tgaacgctcg gagttcatcg caagacatgc caagaacact ccgaccgcac cattcgatgg
     1201 cacccgaatc cggctgcacc agccttatta tgctcttcag cgctgttgac ttgccgcacc
     1261 cggatgggcc cattactgca acggtctcac cttgctcgac gttaaggctg aacccagtga
     1321 gggcctgccg ggaaccgtaa gtcttcctca agtcgcgtat tgagagcact gacttgcccc
     1381 tcccttcgaa gtagccccag atgacgccgt atcgccaaac acaagacgcg gccgattcag
     1441 aatcgaaagc atgcctggtc tgcctgccgg cgccacaatc tccctcatca tctcggaggg
     1501 gctcatcccc aaagctatgc atgcttccac ttcatctgcg ttcacaggcg acaggcgtgg
     1561 ggcggcccag gcaaccacga gacccatggc catacccagc acaacgaagg ccggaaaccc
     1621 catcgatgcc agggttgcgc gggttacggc agctatcgtg cccagcagaa gagcgcctac
     1681 acatgagccg tacccgtaca ccgccagcgc atcccggcgg gacgtgccct cgccatgtag
     1741 cgccatggca ctcgcgcccg cgatcaccgt ggagtggtca agtatgagcg aaagcagaac
     1801 aatggcgcag gcggctaggc cggatatggt ccccttcacc gatttgagca gcgttcggac
     1861 ctcactcctc acgccaggct gcactacccc gggcggaaga atgtacacag ctgcgtccgt
     1921 tcccgcggcc ttccctatca ccttagccag ctgctcctga ggcgtgtccg attcaacaag
     1981 gagttctacc ttgcctgacg actcctgctg ctcctcgatg aactgatcta tcatccttat
     2041 ggaacgggcg gcgccttcgc cagaaaaatc cggcgtccgg gattgcacgg cctcaagctg
     2101 cttcgatatc ccctccacat ccaggccggc caggctgtca agatcggacc gggcggcctc
     2161 cagctgctcg ccaagagcag cagcgtccag ctcctgcagc gtagagagaa tgcctgcggc
     2221 cgcccccgac atctcatcca gcagggttgc aacatccccc gcgccgctga ccgccgattg
     2281 aaccgtctcg agtttgccgg caaacgactc cagccggctc tgccatagcc tcaagttgct
     2341 gagcagagga tcgtagtgcg aagtgaacat cgtggcggcc tcggctgcag tctccaaggt
     2401 ccgaattgct tctagggcct tcgaagtaag ctcggccagg gaacgcacag acgccgggtc
     2461 aatgcgcatt ctagctggat cagtccccac tccgtccccg atcccgaccc gttccagcgc
     2521 ccgctggagc ctgatcatgg aatcgatggc agtctcgtag gcgtccagag cctgctccca
     2581 gccgcgcgtt gcctcaagca tggcgcctgt cgcctggcca agacgcccga gcacagcaga
     2641 ggcttcctga agtgagtttc ccagttcacg gctgggcaga acgagccggc ctgtaccgac
     2701 gcgaggacca accatctctc cgtccatcgc atatgcaatg agctcgccgg tcgcaccgtc
     2761 cacgtccgag ttgattacaa ttcctcgggc cgcagaatcg gatacctcgg tcaaggcgac
     2821 cacgatatcg ccgtctccga cagctgatcc gccccgtccg ggcgtcgccg acatgataag
     2881 gctctcttcg ggctgcacat ctgcgtcttt cagagttatc acggcctgcg gcgcccacgc
     2941 ctctatgaaa cgcttcatct ccttaagcgc ccggccgagc tcgcgagctt cctgcgctgc
     3001 gccgtccgca gtgacgtcgt gcactgcgga agctccgtcg cctgccatga ccttatcgat
     3061 caaggactgg cggtcaccgt ccgacacttc gacgctctcg ggcctcacat cgaccagatg
     3121 gtaccttctg agaatgccct cggctgtctg cgccacagca cgagcagagt cgcctgaatc
     3181 gcacacaagg tggatattgc ctccgtcctt caaagtaaag cgtacgcctt cgatcgcggc
     3241 caactgcggt cggatgatcg gcgccacccg ggcgcccacg ccgctcacag tgaccttagg
     3301 ctcaagtacg attgtgtatc ctgcaaaccc cggcacttcg gtcgcggcgc gcgtcagccg
     3361 cgtcaccgta gcttcgtctg tatggggcaa cgacacaaag aagttggcgc gagaaagcac
     3421 cgtaacgctt tgcttcagcc gtgcgcctgg aaaatcccgg cgaagcatct gaccgagggc
     3481 gcgggctgcc tgatgccatg cctcctgtcg gacatgtatg atcaagtcgt attcgccgta
     3541 gtcacccaca atgcccgaca ctgtatcctc gaagaagcga tcagcggcag cggacacagt
     3601 cgccgcagcg atcgaaccta cgaccacagt gatgatcaga acgccgacca catcgcggat
     3661 gaacccgcct cgcaaaaggg cgtacatgca gctaaacccc ccattcaccc gcaattatag
     3721 catggggggt agccttactg aatgtaatat ccttgcctga ctggcaatat cctgttttac
     3781 tcttcgatat cgaaggtgat ctccatcttg gtgccttcag ctgctaccat tgtctgatcg
     3841 gtatgcaact cggcacggtc gcactttatg ctctccttgt tctctccctc aataaacact
     3901 gacccagtca gaatcagcat cttctcagag ttcttgtagg atgcgtgtgc ggcttgggcc
     3961 cgctgcttat ctctggtcat cgacacgttc cctgatgcgt tgaagtcctg cgtgtccgtg
     4021 aagatctcca tagcggtgca ggtcaacgtg atcaccgaca ctttcggttt gcccgcctca
     4081 tctttttcaa cctgcgtctc acgtttctgc agccgaacgc tgcctgtcac cgccgctatt
     4141 ttcttcttta tgttcagatc cattgagtca cagccgatcg tcacgtcccc ctgaacaagg
     4201 gctaccttcc caccaagctt ggcaataccg gcgtccatgt tcaccgtcgc acggtcagcc
     4261 gtgatcacta tgtcgtcata ctctatcttc agcccgccct cgaacaccgt atcgcccttg
     4321 gcacccttgt gcaccacgga ttggtacgcc gtaaccttaa ccggcttggc ctgcaccggt
     4381 atggagagtg caaacacggc caccacaaat aacaaaatgc ctgatcgcaa cacccgcctc
     4441 acgtatctcg ccccccccac tttactcttc agaatcatcc aactcgaact ggatggtagt
     4501 tctccctcgc gcctcgaaag actcgccgtc ggagctgaag tgaatatcgt cgccctccat
     4561 ttgaaagctg cccttgcctt ccgcctcgct cacgagcacg tccccagctg tatacatctt
     4621 gtcggcctca atatccacct caagctgact gactgtcact acgccggcag aggttgagat
     4681 ctcggtcttg cccggtatga gcaccttgga ttgcttcgga tcccaaacaa cctcatcagt
     4741 ggcaagtaga tccttgcctc ccgacgtcac tttcacccct ccgtccagac gcaactggcc
     4801 tgtcttgctg tcatacaagc ctcggccggc gctgaaggtc agatacggct cgccgctacg
     4861 gtagatcgcg ccctccgtga tcttatacat ctctgcccga ccgtcgccgg cccccgtttc
     4921 cacccgggcc gccgaaagca tccacacccg ttccccgccc tctctcccct cgaccgcaac
     4981 accatcgaac agcaggtcgc gggaggctgg ttgcttggtg ccgcgccccc tttcgctcgc
     5041 agggtcggca tcgggcggat ccggtctgtt gatcacgaag gcgtatatca gggcgcaggc
     5101 gagaagcacc gccactgaga tgctgaccgc cttcaccagc ttcgcgctca cgtgccgctc
     5161 cccccttgat gaccccgagt tactgcccga agatcactcc atcttcgccg agcccgaagc
     5221 caagaggcgt gcccgggaag gccttgaagc ggaactccag ccacacccgc cgtgtggtgt
     5281 attgatacgt taccgctatc tccctacaat ggaggtcgcg cgttatccca atatcgcccc
     5341 ggacaaggcc cccttttacc aggtcgtaca cggccaccca ctgtatacgc caatctggca
     5401 gagggcgcat atccgccgac gactctaccc gtgtcatccg cccgcgcgcg aagtcgtagt
     5461 agcctccggc tctgagctga aattcgggag acggctgtac tactacccta ccgagtagag
     5521 atttgggcaa tgcctgttgt atgtcgtatc cg
//
LOCUS       DAOTAC010000167         5545 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_12310_length_5545_cov_4.496721, whole genome shotgun sequence.
ACCESSION   DAOTAC010000167 DAOTAC010000000
VERSION     DAOTAC010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5545
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12310_length_5545_cov_4.496721"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="PLM74_07820"
     CDS             complement(<1..286)
                     /locus_tag="PLM74_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43017.1"
                     /translation="MRLGRYATAAAVSLLFCVALAGCGAGGNQKKLLSYFPSSEGFTW
                     EYAGTDDYAQTMGIDSIYKASSGEVTYGISGETVVVGVDDAEEESEGEFAL"
     gene            1198..2715
                     /locus_tag="PLM74_07825"
     CDS             1198..2715
                     /locus_tag="PLM74_07825"
                     /inference="COORDINATES: protein motif:HMM:NF016364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HON43018.1"
                     /translation="MSRSGRAPRIGAIVLILALCAAVLAEGAPKRGFETYVGYRDMLE
                     VTSGIEWVDEADGRLDIAGRSRYADVIVIASLEGFSAERHTKMTVDSGGDSRGTVWFV
                     DGPGWYRMSVATRPPGRQIYAIVCSFSVRNTSLVTPLAPTEYAGYGDDLTIESPRGDT
                     NVLKETLTVRGWSRYARVRAIVEHLETGEVRTYTVRASSDGSFVFPIPLEVGFGPCEV
                     KLSSLQVDADTWRGAAVYNVVSAMPSIYMIEPAYQYGTIFATGRFRIRGMAPDSPTVI
                     VETTSEDGGKTHWRADVAVMNGAFETWVDMPPGATAGDVFQLRVSMPGAGTEDPGSRR
                     AYTVAFTPRRPDPKTTESWVAHSTNEAIRDLATAIVGDKTDHYEIVQAIHDWVARNIA
                     YDVRGAKTLKPGPVDALATLERRESVCAGYANLMAALLRAAGLRVWVLIGNADNGITV
                     VKHAWNEVEVNGRIVAIDATWDSGGVEGWSFVRRYSRAFFDPQPDQLAFTHFEDR"
     gene            2979..4868
                     /locus_tag="PLM74_07830"
     CDS             2979..4868
                     /locus_tag="PLM74_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43019.1"
                     /translation="MTGTAKTGLAKGSRLSGRRSLAVATAIVLTIVAMGLPVIAQEHP
                     LSAWRLDEWGKWTGLPAVNPYATITGLGSSQGWGNAGASIGITARAVFEPDLSRAEIS
                     AEFVPVSRAIIASDSRLFIAPLGQRLEWKVIVTVRNPNDVSMTNVRVHNQFGKAFRAA
                     LTGQSVGDARMADADVKGLLPSSSGFTWDIGYLGPGDAAKAELTVTTNQSGGRAEFAE
                     EGVYSLDTGFQLTYRLLGKDEVRNTAPCSVIARRNPSALRSDGEPYGVVQKPIERDLV
                     LPERPISHGPVPVRPGAEKPIVIEPHSQQGPAVTRRVAPELSAEELGQLEAEVAQGYA
                     TKNVIRDDDISRFTVTATASEGNITEEGGKFVVPVGEYVEWVVDLKVHNPFFVAYWYY
                     WSARLEFGPGLDVECLTRSTNSSVTVTPELERTVVTWRNCGNLDEYESAEAKLKVSTR
                     NPAHYSTSGEFPLVQNMQLQYDGIWLLVVPIAGHVCLQPIYVIAKAEPKASVSASATR
                     LDWRVRKPGTYAALATEITATGSGRLQVQFSGFRDLARTDGIAGTIAAFYGFGDDLAA
                     VETGGWVSAADLNNQTRYIYLSHPAPLLMWSKISVGEEVSSAEYESKGVITFIVSNNW
                     EGEGT"
     gene            4941..>5545
                     /locus_tag="PLM74_07835"
     CDS             4941..>5545
                     /locus_tag="PLM74_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43020.1"
                     /translation="MKRLLVLFAIMALIFVPAMASAALPGEPLPLGAVEVEHWELAAD
                     GITWIHKDPGEGLARCWRSGGDQGECNQTSWVVDFTHHAAMAQWSKWSIGGTRWDWRI
                     LKPGTYAADCIEFKLQSNNSVLINYENFADLLRTTEGGVKQTIDTYYSFGESIKQAES
                     NGWVRAEDLNEDDDIIPDSAVLHVNYVTKLFNKIVVENCNSS"
BASE COUNT         1203 a         1554 c         1709 g         1079 t
ORIGIN      
        1 ccagggcgaa ctcgccttcc gattcctctt ctgcgtcatc tacacctaca acaacggttt
       61 cgccggagat gccgtaggtg acctctcccg acgatgcctt gtagattgag tcaatgccca
      121 tggtctgggc atagtcgtct gttcctgcgt actcccatgt gaaaccttcg cttgatggga
      181 aatacgacag caacttcttc tggtttcctc ccgccccgca accggccagc gcgacgcaga
      241 atagcaggga taccgccgct gcagttgcat acctcccgag cctcatgaac ctatcaactc
      301 ctcttcgcgt gatgcagtca tgctatcagc cggcgctggc tccggcgccg gcccggcatg
      361 attagctcct cttcctaagc gccgactcta cggcgcaaat ggcctcctgg tcgagacctg
      421 agctttcgac acactcccac acactccggc caatccatgg ccagagtagg gtggcggcga
      481 gtctggttcg agtcagctgc gacggccata acgacgcctg ggcgcaggcc gaggtgccga
      541 tagggctttg ccacagcaat atgctgaggg cgcagcgcgc tccggacgga acaggcttcg
      601 aaaacacgtc ggccctggtt attctcaatc gccaccggcc tttcctttcc ggcagggcgc
      661 acacgccata gccctggagc atgaaatgtc gggccacatc gcacactatt gaccccaggt
      721 ccgccgcaga actcgggaag acacggctgg ttccgcgcgg cttgtgattt acggttatgg
      781 acatccccgt accacctcga ctgcacaacg tatggacccg cagtacaata ggataccttc
      841 cgtacgtcag ccggttttcc tccctgcatt gacctcgttc gcgaaatcgc gcggataccc
      901 aacaccgaac gggcgtgatt ttgcgggcgt ggactaggcg cagcgacacc gttcgtggag
      961 attgtgcgcg cgtctcgaag cgttctgcgc ggatggcctc accaaacaac ggaatcctgc
     1021 ggaccgattc gtttctccca acagagtcct gcccaaggaa ggattttctg ggcaagtgca
     1081 ccgggatgat tctacatggt tcacccgtcc gacacggtat tatcgggctg aagtccgggt
     1141 tctatcgact ggcagcgcaa tccggcaagc cggcgctttg atgcgggggg tgctggggtg
     1201 tcacgaagtg ggcgggcgcc gcggatcggc gcgattgtcc taatcctggc tctgtgcgca
     1261 gcggttcttg cagaaggcgc cccaaagcgc ggctttgaga cgtatgttgg atacagagac
     1321 atgctcgagg tgacctcagg aatcgagtgg gtcgatgagg cagacggcag actcgatatc
     1381 gctgggcgaa gccgatacgc ggacgtgatc gtaatcgctt ccctcgaagg attcagcgcc
     1441 gagaggcaca cgaaaatgac agtggactcg gggggcgatt ctcgcggaac ggtatggttt
     1501 gtagacggac ccggctggta ccggatgagc gtcgcaacgc gtccaccggg ccgccagatc
     1561 tacgccatcg tctgcagttt cagtgtgcga aacacctcgc tcgtgactcc gctggcgccc
     1621 actgaatacg cgggctatgg cgacgactta acaatcgagt ctcccagagg agatacgaat
     1681 gtcctcaagg agacgctgac ggtgcgggga tggtcgcgct acgcgcgggt tcgcgctatc
     1741 gtcgaacacc tggaaacggg cgaggttcgt acgtataccg tgcgagcgag ctcggacggg
     1801 tcgtttgtct tcccgatacc gctggaggtc gggttcgggc cgtgcgaggt gaagctctcc
     1861 tcgcttcagg tcgatgccga cacatggcgc ggcgccgccg tctacaacgt ggtatcggcg
     1921 atgccctcga tctacatgat cgagccggca taccagtatg ggaccatctt tgccacaggc
     1981 aggtttcgaa tacgtgggat ggcccccgat agccctacgg ttatagtgga aacgacttca
     2041 gaggacgggg ggaagaccca ttggcgggct gatgtagctg tgatgaacgg ggccttcgag
     2101 acttgggtgg acatgccccc cggggccacg gcaggagacg tgttccagct tcgggtgagc
     2161 atgcccgggg cgggcaccga agatcccggg tcgcgccgcg cctacacggt agctttcact
     2221 ccacgaagac ctgaccccaa gaccacggaa tcctgggtcg cgcattccac gaatgaggcc
     2281 atacgggatc tagcaaccgc cattgtgggc gacaagactg accattacga gatcgtgcag
     2341 gcgattcatg actgggtcgc caggaacata gcatacgacg tcaggggagc gaagacactg
     2401 aaacccgggc cagtggacgc tctagctacc ctcgagcgcc gcgagtcggt gtgcgcaggc
     2461 tacgccaacc tcatggccgc actgctccgc gctgccggac tacgtgtgtg ggtgctcata
     2521 ggcaacgccg acaacggaat caccgtagtg aaacatgcgt ggaacgaggt ggaggtcaac
     2581 ggacggattg tggccattga cgccacgtgg gactccgggg gcgtggaagg gtggagtttc
     2641 gtccgccgct acagccgggc attcttcgac cctcagccgg atcagctggc tttcacacac
     2701 ttcgaggaca gataatcaag attcgatgta agaaagttac gaaatctgtg ggaaggaatc
     2761 ccattagtcg cggagaatca gtcaaaggaa cgatgcctgg tgtggtcggc cccgacccgc
     2821 caggcgtcgg acgcaaaagg ggagttcgag gaatcctggc catagatgca gcaaatcggt
     2881 gaggtacgcg ggaatcgggg ctctgcgaat aacagggatc gcaatatgcg caatattcgg
     2941 taaatatcga ccacacaggt tgaccgggag ggttcggtat gactgggacc gccaagacgg
     3001 gactggcgaa aggctcgaga ctgtcgggcc gaaggagtct cgcagtggca actgcgatag
     3061 tcttgacaat cgtcgctatg ggactgccgg tgatcgctca ggaacacccc ctatcggcct
     3121 ggagactgga tgagtggggc aagtggacgg gccttcccgc ggttaatccc tatgccacca
     3181 taaccgggct tggctctagc cagggctggg gcaatgccgg cgcttctatc ggcataacag
     3241 ccagggcagt gttcgagccc gacctgagcc gagctgagat cagcgcagaa tttgtgccgg
     3301 tgtcaagggc catcatcgcc tcggattcga ggctcttcat agccccgctc ggccagcgac
     3361 ttgagtggaa ggtcatcgta acagtgagga acccgaacga tgtgtcgatg accaacgttc
     3421 gcgtccacaa ccaattcgga aaggcgttcc gagccgccct gactggccag tcggtcggcg
     3481 acgcgcgcat ggccgacgcc gacgtcaagg ggctgctccc atccagctcc ggctttacat
     3541 gggacatcgg ctacctgggc cccggcgatg cagccaaagc tgaactgacg gtgactacca
     3601 accaatctgg aggccgcgcc gaattcgccg aagagggcgt atactccctt gacaccgggt
     3661 ttcagctcac ctaccgcctg ctgggaaagg acgaagtgag aaacactgcc ccatgttcgg
     3721 ttattgcccg caggaacccg tcagccctta ggagcgatgg tgagccgtac ggggttgtgc
     3781 agaagccaat cgagcgcgat ctggtgttgc cggaaaggcc gatttctcat ggccctgtcc
     3841 ctgtgcgacc tggagccgaa aagcccattg tgatagaacc gcatagccag cagggccctg
     3901 cggtgaccag gagagttgcg cccgagttga gcgccgagga actggggcag cttgaggccg
     3961 aagtagccca ggggtacgcc acgaagaacg taatccggga tgatgacata agccggttta
     4021 ctgtcactgc gactgcgtca gagggcaaca tcaccgaaga aggcggcaag ttcgtggtgc
     4081 cggtgggaga gtatgtagaa tgggtcgttg atctcaaggt gcataaccca ttcttcgtcg
     4141 cgtactggta ctattggtca gctcggcttg agttcggtcc cgggctggat gttgagtgcc
     4201 tgacgcgcag cacgaactcc agtgtgactg tgaccccaga attggagcga acggtggtca
     4261 cgtggcgcaa ttgcgggaat ctggatgagt acgagagcgc agaagcaaag ctgaaggtat
     4321 ccactaggaa tcctgctcac tattccacat ccggtgagtt ccctcttgtg cagaacatgc
     4381 agctacaata tgacggaata tggttgttgg ttgtcccaat tgcagggcat gtctgcctgc
     4441 aacctatata cgtgattgcc aaagcagaac ccaaagcctc agtctccgct tcggcaaccc
     4501 gacttgactg gcgcgtacgg aaaccgggga catatgccgc cttggctaca gagatcactg
     4561 ccacaggctc gggccgactg caagtccagt tctctggctt tagggatctg gcaaggaccg
     4621 atgggattgc ggggactatc gcagcattct acggattcgg cgatgatctg gctgctgtgg
     4681 aaacgggagg gtgggtatca gcggcagacc tgaacaatca gacaaggtac atatatctat
     4741 ctcaccctgc gccgcttctg atgtggtcga agataagtgt gggcgaagaa gtctcgtccg
     4801 ctgagtatga gagcaagggg gtgatcactt tcatagtcag taacaactgg gaaggcgagg
     4861 ggacctagac gccctggacc ttgacgggtt aaggcccgtc acgtcgactc aaatcaatac
     4921 ttcgaggagg ccggacatca atgaagagac tgttggttct gttcgcgatc atggcgctca
     4981 tcttcgtccc agctatggct agcgctgcgt tgccaggtga gccactgccg cttggcgcgg
     5041 tggaagtcga gcactgggag ctcgcggccg acggcataac atggatccac aaggatccgg
     5101 gtgaaggact ggcacgctgt tggagaagtg gcggcgatca gggcgagtgc aaccagacga
     5161 gctgggtagt cgactttacc caccacgctg ccatggccca gtggtccaag tggagcatcg
     5221 gcggaactcg ttgggactgg cgtatactca agccgggaac ctatgcagct gactgcattg
     5281 agttcaagct gcagagcaac aactctgttc tcattaacta tgagaacttc gctgatctcc
     5341 ttcgcacaac agaaggtggg gtaaagcaga ccattgatac ctactacagc ttcggagagt
     5401 caatcaaaca ggccgagtcc aacggttggg tcagagcgga ggacctgaac gaagatgatg
     5461 atatcatccc cgacagcgcg gtcttgcacg taaactatgt caccaagctc ttcaacaaga
     5521 tcgtggtcga gaactgcaac agctc
//
LOCUS       DAOTAC010000168         5522 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_12377_length_5522_cov_4.324492, whole genome shotgun sequence.
ACCESSION   DAOTAC010000168 DAOTAC010000000
VERSION     DAOTAC010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5522
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12377_length_5522_cov_4.324492"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="PLM74_07840"
     CDS             <1..644
                     /locus_tag="PLM74_07840"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HON43021.1"
                     /translation="THVARTGDIGMIKIVSEGGIAAGVRRIEAVTGLGALDYVNRTRS
                     ALHEVADILKVSEDSVSARVDRLMQDLHDKEREVHRLTQRLAAGSIDSIVAAREMVGG
                     VAMVAAPVEAPDAEGLRNLADQIRDKLGSGIVLLGAVTGDKLGFVACVTRDLVELGYN
                     AGAIVKEAARAAGGGGGGRPDMAQAGGRDVSKLDAALAAGVDAVRARQAAHSM"
     gene            707..964
                     /locus_tag="PLM74_07845"
     CDS             707..964
                     /locus_tag="PLM74_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015311508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HON43022.1"
                     /translation="MTGHWDETVKYPALGAEEVTPVRKVLTEVTAALVAKGYNPVNQL
                     VGYLLSGDPTYITSYKGARNLIRKVERDEIIEELVRSYLRK"
     gene            984..3311
                     /locus_tag="PLM74_07850"
     CDS             984..3311
                     /locus_tag="PLM74_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43023.1"
                     /translation="MTAVLLAAVLLAWAANGRASGSDRVAVADRDGHRVVVILADGLT
                     WQDCRVRGGTALTRFLSSAAVGCANARTADRGEPLLSACATLGAGRRMTAPESAGYGL
                     GLNEVLHGETAVDVYLRRTRRLPPWGDQREGGADGMLPATAAVCTAWPELERANAESP
                     YRGKPGLLGESLRRAGMAAAAVGNSDDQELVCRPAVLIAMDACGATPWADVGTHTVAS
                     DPHAPFGMVCDVGAMAEAAWAAFSAETETEAAGAATDAEVPPVAVLVLDMGDFARLRR
                     YAVNLSPEAYYVQLGLCYERLDALLVELEHSLGPPSEGLAYVLISPSSESGLAPVAVA
                     GWSYGAGLLTSPTTRRAGLVTNLDIAPTILAGLGVDPAWECDGAAMAQATVAADAPGG
                     LTDHVALTMLMEQTLALAAQARGPVLRLFIGLLVVAVFGLLGVIVLGRRVPGWAVRPV
                     RALLVAAGSAPLVMLAGPVVGVQGTAASTVMLIIGCAAMALLMVRVSGSAMWSVVILG
                     GATASVITIDAFTGGKLASTCLLGHSAVLGARYYGIGNELMGVLVGSAAVAGAGLSRM
                     AWPAAAAAAAAMVMLGHPSIGANFGGMVSAAVTSAVIAAMAAGRLPLSGGSARGRRER
                     LVAMAGVGLAILGASAILILWDSRSPRASHIGRAWRTLTQRAGGSGQLALIALRKLAM
                     NIRLLRYTAWTQVLIAFLAILGAMAAGAHGVFQRFRRDHFSLYAALMGGLVGALAAMA
                     VNDSGVVACATLAMMPTLVILGYAADELAARGARE"
     gene            3328..4275
                     /locus_tag="PLM74_07855"
     CDS             3328..4275
                     /locus_tag="PLM74_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HON43024.1"
                     /translation="MDIRELGLTGICVSRLCLGTLTMGPLQANIGVERGAELIRHAVD
                     MGVVFFDTAELYGTYGHLRKAMQEIPRESVVIASRCYAYTYDGMRRSLERALTELGTD
                     YIDIFGLHEQESRLTLKGHADAIQCLLDAKQEGLIRATSVSTHTVEVVRAVSAMREID
                     VVHPIFNKRGIGIIDGNTAEMADAIQAASRRGAGVYSMKPLGGGHLFREAPEAIQWVL
                     GHDSVHSMAIGAKSCDELDADVAIASGLKVPDEVLRYLAGEKHLLIEEWCSKCGACVE
                     SCRHGALRLGAKRAEVDADRCVLCGYCVAYCRDFCIKVV"
     gene            4287..4706
                     /gene="ruvX"
                     /locus_tag="PLM74_07860"
     CDS             4287..4706
                     /gene="ruvX"
                     /locus_tag="PLM74_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HON43025.1"
                     /translation="MVRIGLDVGEARIGVAVCDELGLVASPRTVIHSKGWGSDIARVG
                     ELVLEVGAGEIVVGLPVNMNGTLGPAAARVEEFCRRLRESVSVPVVTWDERLSTAQAS
                     RVLIQADLSRKRRRDVVDKTAAAIILQAYLDNLASRG"
     gene            4833..5162
                     /locus_tag="PLM74_07865"
     CDS             4833..5162
                     /locus_tag="PLM74_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HON43026.1"
                     /translation="MPDEENIITLVEEDGKERDYAIDEIIEIDDAKYAVLIPVECFEE
                     DEEGICEEAIIMKYEVDEKGDEYLSDIEDDEEYERVIEALEAFEEDDYDYDDDDEDED
                     DDDEEEF"
BASE COUNT          976 a         1524 c         1919 g         1103 t
ORIGIN      
        1 gcacgcatgt ggctcgcacg ggcgatatcg gcatgatcaa gatagtgtcg gagggcggca
       61 tcgcagccgg cgtcaggcgg atagaggctg ttacaggcct cggggcgctt gactacgtga
      121 accggacgcg ctcggctctg cacgaagtcg ccgacatcct gaaggtctcc gaagactctg
      181 tgtccgctcg cgtcgatagg ctcatgcagg atctgcatga taaggagcga gaggttcacc
      241 gtctcacgca gcgcctggcg gcgggaagta ttgactcaat tgttgcggcg cgtgagatgg
      301 ttgggggcgt agccatggtg gcggcgccgg tggaggcgcc tgacgccgaa ggcttgcgga
      361 atctggcgga ccagatccgt gataagctcg gatcagggat agtcctgctg ggcgcagtga
      421 ccggtgacaa gctcggattc gtggcgtgtg ttactcgcga ccttgttgaa ctgggctaca
      481 acgccggcgc tatcgtgaag gaagccgcgc gcgctgcggg cggcggcgga ggcggcaggc
      541 ccgacatggc gcaggcggga gggcgcgatg tgtccaagct cgacgctgcg ctggcggcgg
      601 gtgtcgatgc agtgcgcgcg cgccaggcag cgcattcaat gtagaagcgg agcattcggc
      661 gtcttggttc tgtgaacact attaccagcc cgaggtggtg ttttcgatga ctgggcattg
      721 ggatgaaacg gtcaagtacc cggctctggg ggcggaagag gtcacgccgg tcagaaaagt
      781 gctgactgag gtgacggccg cactcgtggc gaagggctac aatcccgtca accagctcgt
      841 gggttacctg ctctcgggcg accctacata catcacgagc tacaagggcg ctcgcaacct
      901 catccgcaag gtggagcggg acgaaatcat tgaggagctg gtccgaagct atctgcgcaa
      961 gtgatagatt gcgcaggcat acattgaccg cggtcctact ggccgcggtc ttgttggcct
     1021 gggcggctaa tgggcgcgca tctggcagcg accgggtggc tgtcgctgat cgcgatggtc
     1081 acagagtcgt cgtgatactg gcagacggcc tcacctggca ggactgccgc gtccgcggag
     1141 ggactgcgct tacccggttt ctgtcgtcag ctgccgtcgg atgcgctaat gcgaggaccg
     1201 cggacagggg agaaccgctc ctgtcggcct gcgcgacact gggcgcgggg cgcaggatga
     1261 ctgcgcctga gtcggccgga tacggcctgg gcctgaacga ggtcctccac ggtgagactg
     1321 ccgtcgatgt ctatctccgc cggacgcggc gcctgccgcc ttggggtgac cagcgggaag
     1381 gcggcgcaga tggaatgctc ccggcgacgg cggcagtctg caccgcctgg cccgagctgg
     1441 agcgggccaa cgccgaatcg ccctataggg ggaagcccgg cctgctgggc gagtcgctga
     1501 ggcgcgccgg catggccgct gccgcagtcg gcaattccga cgaccaagag ctggtgtgcc
     1561 ggcctgccgt gctcatcgct atggatgcct gcggcgcgac gccatgggcg gatgtaggca
     1621 cccatacggt cgcgagtgac ccacacgctc cttttggcat ggtgtgtgat gttggagcta
     1681 tggccgaagc ggcgtgggcg gcgttttcgg ctgagactga gactgaggct gcaggagccg
     1741 cgacagacgc cgaggttccg ccggtggccg ttcttgtgct ggacatgggc gatttcgccc
     1801 gcctgcgaag atacgcggtc aacctgtcgc ctgaagctta ctacgtgcag ctggggctgt
     1861 gctacgaacg ccttgatgcg cttctcgtag aactggagca ttctctgggg cctccttccg
     1921 aggggctggc ctatgttctc atttcgccgt cttctgagtc ggggcttgcg cctgtagctg
     1981 tcgcaggctg gtcgtacggc gccggtctgc ttactagccc cactacacgc cgggctgggc
     2041 tcgtgaccaa cctggacata gctccgacga tcctcgcggg gttgggcgtc gatccggcct
     2101 gggaatgtga tggcgcagct atggcgcagg cgacggttgc ggcagatgcg cccggcggac
     2161 tgaccgatca tgtggcgctg actatgctca tggagcagac gcttgctttg gcggcacagg
     2221 cacgcggacc tgttcttcgt ctattcatag ggcttcttgt tgtggcagtc tttggcctgt
     2281 tgggagtgat cgtgcttggg cgccgcgtgc ccgggtgggc ggtgcgaccg gtgcgggcgc
     2341 tgctcgttgc ggcaggcagt gcgcctcttg tgatgcttgc ggggccggta gttggggttc
     2401 agggcacagc tgcgtccact gtgatgctca taatcgggtg cgcagcgatg gcgcttctca
     2461 tggtgcgcgt atccggctcg gccatgtggt ctgtggtgat ccttggcggg gccactgcct
     2521 ctgtgatcac catagacgcg ttcaccggcg ggaagctggc gtccacttgc cttttgggcc
     2581 attcggcggt tcttggcgcg aggtactatg gaatcggtaa cgagctcatg ggcgtgttag
     2641 tggggtcggc tgcggtggcc ggagcggggc tatcgcgcat ggcctggccg gctgcggcgg
     2701 ctgcggcggc cgccatggtc atgctggggc acccttcgat aggcgcgaac ttcggcggca
     2761 tggtctcggc tgccgtcacc tcggcggtta tcgcggccat ggctgctggg cgccttccac
     2821 tgtcaggcgg ctcggcccgc gggcggcgcg aacgtctagt tgccatggcc ggtgtgggcc
     2881 tcgcaatatt gggcgcttcg gctatcctga tactgtggga ctcgcgtagc cctcgggcgt
     2941 ctcacatcgg acgggcgtgg cgcacattga cgcagcgggc tgggggttca gggcaattgg
     3001 ctttgatagc cttgcgcaag ctggcgatga acattcgtct actaagatac acagcctgga
     3061 cgcaggtgct catcgcgttc ctggccatcc tgggggcgat ggcagccggc gcgcacggcg
     3121 tctttcagcg tttcagacgc gatcatttca gcctgtacgc tgcactcatg ggcggtcttg
     3181 taggagcgct tgcggcgatg gcggtgaacg attcgggcgt agtagcctgc gctacgctgg
     3241 cgatgatgcc gaccctggtc atcctcgggt atgccgccga cgagctggcg gcgcgcggcg
     3301 ctcgcgagtg actgaaggtg attgactttg gacattcggg aattgggact cacagggata
     3361 tgcgtgtcgc gactctgctt gggaacactg actatgggtc ctcttcaggc gaacataggg
     3421 gtagagcgtg gggccgagct catccggcac gctgtcgaca tgggcgtcgt cttcttcgat
     3481 actgcggaac tgtatggaac atatgggcat ctgcgcaaag caatgcagga gataccgcgg
     3541 gagtcggttg tgatcgcgtc gaggtgttac gcctacacct acgatggcat gcgccgaagc
     3601 ctggagcgag ccctcactga actggggacc gactacatcg acatcttcgg cctccacgag
     3661 caagagagcc gtctcacact aaaggggcat gccgatgcca ttcagtgcct tcttgacgcg
     3721 aaacaggagg gtctgatccg cgcaacgagc gtgtccactc atacagttga ggtcgtcagg
     3781 gccgtctcgg caatgcgaga aatagatgta gttcacccca ttttcaacaa gcgcgggatt
     3841 ggtataatag atgggaacac ggctgagatg gccgatgcga tccaggcggc cagccgccga
     3901 ggggccggcg tttacagcat gaaacctctc ggaggtgggc atctatttag ggaggcgccc
     3961 gaggccatac agtgggtgtt gggccacgac tcggtgcact cgatggcaat tggcgccaag
     4021 agctgcgatg aactggacgc agacgtagcc atcgcatcgg gcctcaaggt gcctgatgaa
     4081 gtccttcgat accttgcagg agaaaagcat ctgctcatag aagagtggtg ctcgaagtgc
     4141 ggagcatgtg ttgagtcctg caggcacggc gcgctcaggc ttggcgccaa gcgggcggaa
     4201 gtcgacgcgg ataggtgcgt gctctgcggc tattgtgtcg cttactgcag ggacttctgc
     4261 ataaaggtag tctaggaggg cacagtttgg ttcggattgg gctcgatgtc ggagaggctc
     4321 gcatcggtgt cgctgtctgc gacgagctgg gcctggtcgc gtcgccccgc acggtgatcc
     4381 atagcaaggg gtggggcagc gacattgcac gcgtgggcga gctggtcttg gaggttggcg
     4441 ccggtgagat cgtggtgggt ctcccggtga acatgaacgg tacactgggt ccggctgcag
     4501 cgagagtcga ggagttctgc cgtcgactcc gtgagtccgt atcggttccg gtcgttacat
     4561 gggatgagcg tctcagcact gcccaggcat caagggtcct gatacaggcc gatctgtcgc
     4621 gcaaacgacg gcgcgatgtt gtggataaga cagccgctgc gatcatactc caggcttacc
     4681 tcgataacct ggcgtcgcgg ggctgacccg tcgccactgc cacgattggc gtttcgtgcg
     4741 ggcatggccc gcaggcattt gcctcagaag gctgcgaagg ggatatcatg ttaccttggc
     4801 agaccaaata gggccagatt ggagtgatca gcatgccaga cgaggagaac atcattacgc
     4861 tcgtagagga agatggcaaa gagcgcgact acgcaatcga cgagatcatc gagattgacg
     4921 atgctaagta cgctgtgctc attcccgtag agtgcttcga ggaagacgaa gaagggatct
     4981 gcgaggaagc catcatcatg aagtacgaag tcgacgagaa gggcgacgag tacctgagcg
     5041 acatcgagga cgatgaggag tacgagcggg tcatcgaggc tcttgaagcg ttcgaagagg
     5101 acgattatga ctacgacgac gacgatgagg acgaggatga cgacgacgag gaggaattct
     5161 agtctcgtct acccactttt catccgtccg tttccatgcc tggctgacga aggccccctg
     5221 tgtgggggcc ttcatggttg acgtggcaag attcctgcag ctcatgtggc agaggtggag
     5281 ctttgctcag cagatcacgc gagacgtcct tgcaggactc ctgcggttca tttagcaagg
     5341 gcggagtgtg gcggcggatg ctatgaactt cattcgtagc gcaaccccca ggccatcccc
     5401 tgccaggcga gcccatcccg tgctatgcca tcccaggcga tcccatgccg cgccatgcca
     5461 tcccaggcga tcccatgccg cgccatgcca tcccagacca tcccctgtaa gttttccgac
     5521 ag
//
LOCUS       DAOTAC010000169         5500 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_12435_length_5500_cov_4.791552, whole genome shotgun sequence.
ACCESSION   DAOTAC010000169 DAOTAC010000000
VERSION     DAOTAC010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5500
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12435_length_5500_cov_4.791552"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1054
                     /gene="gpmI"
                     /locus_tag="PLM74_07870"
     CDS             <1..1054
                     /gene="gpmI"
                     /locus_tag="PLM74_07870"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HON43027.1"
                     /translation="LDELEDRLNQLRLGRIATVSGRYYAMDRDTRWDRVKLAWDAMVH
                     SSGQTAHSAREAVERSYAEGVTDEFVMPTVILDEQESPVGPVRDGDSAIFFNFRGDRA
                     RELTRAFNLDEFDGFDRGRRPHVNFVCMMKYLDADIPVAFATPEPHDGLAETLAKAGK
                     AQLHLAETEKFAHVTFFFNGGREEPFPGEDRILIPSPRVATYDLAPEMSAKPIAAEAV
                     DKIGSGKYDFAVINFANGDMVGHTGKLAPAIVAVETVDECIGKVWSAVEAAAGAMIIT
                     ADHGNADEMFDHFSGQPSTAHSLNPVPFILAGTDVQKLREGGDFGDVAPTVLELMGVQ
                     PPAAMTGRSLIAGYGA"
     gene            complement(1000..1416)
                     /locus_tag="PLM74_07875"
     CDS             complement(1000..1416)
                     /locus_tag="PLM74_07875"
                     /inference="COORDINATES: protein motif:HMM:NF024848.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase-like protein"
                     /protein_id="HON43028.1"
                     /translation="MKNVVHVYTTIVPPESGSRWKAVTLVQSSSGRVLAEDVQHLTFK
                     HGAHAESGAPACTAALAAMLQGLLAAKSIRARNVVIYSDRPRTLDALNRQTRVAAAAV
                     GTYLQLRAMAHSFASVEFRYAPYPAISDLPVMAAGG"
     gene            1565..3088
                     /locus_tag="PLM74_07880"
     CDS             1565..3088
                     /locus_tag="PLM74_07880"
                     /inference="COORDINATES: protein motif:HMM:NF013444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HON43029.1"
                     /translation="MSKTQDNGSDLLNIPLEEFDAPLFAALLGYVQQHALRFHMPGHK
                     GGLGFPPEVAEAFKRNVLAMDVTETRGMDNLHLPSGCIKQAQDLAAHTFGADRTFFLT
                     NGTTAGVHAMIMTTCKEGQKIVIPRDAHLSVIGGVILAGLEPIFVYPDVDPEWGVSIG
                     VPTHEYERALSEHPDAKACLITRPNYYGMAGDLADLVDLCHNHGIPLLVDEAHGPHIH
                     LHEAMPRSALSYGADMVVQSTHKTLGSFTGSSMLHIKGERIHPDRVQRMLAILQSTSP
                     SYLLMASIDITATLMRLRGHDLIEHAIRLSDRARERLSQVPGVRVLSRPVMDKCKLSV
                     SMIELGLAGFELKNRLMDEHNARVELADFENVIAFVTYADTQKDVDALVDAMIQVAEE
                     ARSERAVMGPSPRKQGDQARFTKHLFAPAERAMLPRRATLAAHELTRLSDAPGRVCAE
                     VVAPYPPGIPMLYPGEVIDQSHLEIIREAMSLGAIFRGLATDPDGDLVVTVVKAGKS"
     gene            3088..3765
                     /gene="tmk"
                     /locus_tag="PLM74_07885"
     CDS             3088..3765
                     /gene="tmk"
                     /locus_tag="PLM74_07885"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HON43030.1"
                     /translation="MSKGVFITLEGPDGCGKSTQSRMLCDALETAGYDVVRTFEPGGT
                     AIGRRIREIILDTKSGGMCGITEFLLYAADRAQDVHEVIMPALAAGRIVIGDRFFDSS
                     IAYQGYGLGMDVEAVRAVNAIATRGLIPDLTVLLDIDPATGVARATGGRDADRIERRK
                     LAFHTSVRNAYLQMAANEPDRFRVVGVGDLSIDQVHAAVFREVTEFLTNRVSSAVGQY
                     RPGAREV"
     gene            3770..4849
                     /locus_tag="PLM74_07890"
     CDS             3770..4849
                     /locus_tag="PLM74_07890"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HON43031.1"
                     /translation="MHLAEILGQDDAMRILDAEIRSGTMSHAYLLWGPRGSGRTLMAR
                     RLALAANCSLRDQAPPKSLLWYCGECANCTKIVSGVHPDFSVIAPSGSSIKIAQIREM
                     QWRISLKANESELKTWLIDDADQMTEDAQNCLLKVLEEPPGRSLIILVAQDPQMMLPT
                     IASRCHGIRLGTVDTGAMLEWAGRELGLPPERARFLAALSQGLPGRLASLASDHDYFA
                     LRDSIISKVRTVMWCGNGGLALDTSEGLLDELKKASARAQDADAFGEAVPREVLEIAT
                     PTGACDCVAGWFRDLFVLSLTGDEELVINRDYMEALEEDAAHGSPALFGKWTIRVMQT
                     ARALEANASPRLAMDDLMLCLAFAR"
     gene            4874..>5500
                     /locus_tag="PLM74_07895"
     CDS             4874..>5500
                     /locus_tag="PLM74_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008881674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HON43032.1"
                     /translation="MVCVVGVRFKKAGKIYYFDPGEHALAPGIGVIVETARGTEYGEV
                     VVGPKLVPEDDIVQPLKQVLRAATSDDRHQVEENQKKAKRAFDIGLAKIAAHNLPMKL
                     VDVEYTFDSSKVIFYFTAEGRVDFRELVRDLASVFRTRIELRQIGVRDEAKMLGGMGP
                     CGRALCCASFLGDFEPVSIRMAKEQNLSLNPTKISGICGRLMCCLKYEA"
BASE COUNT         1136 a         1545 c         1732 g         1087 t
ORIGIN      
        1 cctggacgag ctcgaagata gattgaacca gctgcgcctg ggcaggatcg ccaccgtaag
       61 cggccgttac tacgccatgg atcgcgacac ccgttgggac cgggtgaaac tggcatggga
      121 cgccatggtc cattctagcg ggcagacggc tcactccgcg cgggaagcag tggaacggtc
      181 gtacgccgag ggcgttacgg acgagttcgt catgcccacg gtgatactgg atgagcagga
      241 gtcaccggtg ggccccgtgc gcgacggcga ctcggccata ttcttcaact tccggggcga
      301 ccgcgcccgc gagctgacgc gagcgttcaa cttggacgaa ttcgatgggt tcgatcgggg
      361 acggcgcccg cacgtgaact tcgtgtgcat gatgaagtac ctggacgccg acattccggt
      421 ggccttcgct acccctgaac cccacgatgg gctggcggag acgctcgcga aagcgggtaa
      481 ggcccagctt cacttggctg agaccgagaa gttcgcgcat gttacgttct tctttaacgg
      541 tggacgtgaa gaaccgtttc cgggcgagga tcgcatcctg attccgtcgc cacgagtagc
      601 cacatacgac ctagcgcccg agatgagcgc caagcccatt gccgccgaag ctgttgacaa
      661 gatcgggtca ggcaagtacg atttcgccgt tatcaacttc gccaacggcg acatggtggg
      721 ccacacgggc aagcttgccc cggcgattgt cgccgtggag accgtggatg agtgcatcgg
      781 gaaggtatgg agcgccgtag aagctgcggc aggtgctatg atcataactg ccgaccacgg
      841 caacgccgat gagatgttcg accatttttc cgggcagcct agcactgccc actccctgaa
      901 cccggtgccg ttcatactgg ccggcaccga cgtgcagaag ctgcgcgaag gcggcgactt
      961 cggcgatgtg gcgcccactg ttctggagct tatgggtgtt cagccaccgg ctgccatgac
     1021 cggaaggtcg ctgatagccg gatatggcgc gtagcggaat tccacgctcg caaaactgtg
     1081 cgccatggcc ctgagctgta ggtaagttcc cactgccgct gcggcaacac gcgtttgcct
     1141 gttcagtgca tcgagggtcc gggggcgatc agagtagatg accacgttgc gtgcgcggat
     1201 gctcttcgct gccaagagcc cttgcagcat cgcggcgagc gccgcggtgc aggcaggagc
     1261 gccggattct gcgtgggcgc cgtgcttgaa cgtaaggtgc tggacatctt cagccagcac
     1321 tctgcctgag ctgctctgaa cgagcgtaac ggctttccac cggctgcccg actcgggcgg
     1381 tactattgta gtgtatacgt ggactacgtt tttcacccta tgcggacctc ctttcgcacg
     1441 tcttggtcga accgaacaag tgttccctat acagacaata acacgaacat atgttcgcgt
     1501 caagcgttgg tctctcggta ccaataaccg ctacgcggcg ggcacgaagg agaaacgata
     1561 cccagtgtca aagacccaag ataacggctc ggatctgctc aatatacccc tagaggagtt
     1621 cgatgctcca ctctttgcgg cgctgctcgg ttacgtccag cagcacgcgc tacgttttca
     1681 catgccgggc cacaagggcg gtcttggttt tccgccagag gtagctgaag cattcaaacg
     1741 caacgtgctc gccatggacg ttaccgaaac gcgcgggatg gacaacctgc acctgccgtc
     1801 tggctgcata aagcaggcac aggacctggc agcgcatacg ttcggagccg acaggacgtt
     1861 cttcctcact aacgggacca cggcgggagt gcacgccatg ataatgacga cgtgcaaaga
     1921 gggtcagaag atagtcatac ctcgcgatgc acacttgtct gtgataggcg gggtgattct
     1981 ggccggcctt gaacccatct tcgtctatcc tgacgtcgac ccggagtggg gggtcagcat
     2041 cggagtgccg actcacgagt acgagcgagc actatcagag catcccgacg ctaaggcgtg
     2101 cttgatcacc aggcccaact attacggcat ggcgggcgac ctcgcggacc ttgttgatct
     2161 ttgtcacaat cacggcattc cgctgcttgt ggatgaggcc catgggcccc atatccatct
     2221 gcacgaagcg atgccgaggt ctgcgctgag ctacggcgcc gacatggtgg tgcagagcac
     2281 gcacaagacg ctaggttcgt tcaccggcag cagcatgctg cacataaagg gcgagcgcat
     2341 acatcctgac cgtgtccagc gaatgttggc catactgcaa tctacgagcc cgtcgtatct
     2401 gctgatggcc tctatagaca taaccgccac tctgatgcga ctgcgcggac atgacctgat
     2461 cgagcatgca atcaggttgt ccgacagagc gcgggagagg ctgtcgcagg tgcccggagt
     2521 acgcgtattg tccaggcccg taatggacaa gtgcaagctg agcgtgtcca tgatcgagct
     2581 gggcctggcc ggattcgagc tgaaaaacag gttgatggac gagcacaatg cccgggtcga
     2641 gcttgcagac tttgagaacg tcatcgcttt tgtgacctac gccgataccc aaaaggacgt
     2701 cgacgctctc gtggatgcaa tgatccaggt cgccgaggag gcgcgctctg agagggccgt
     2761 catgggtccc tcgccgagga agcaggggga ccaggccaga ttcacaaaac acctctttgc
     2821 tcccgctgag cgggctatgc tgcctcgcag ggctacgctg gccgcgcacg aattgacccg
     2881 gctatcggat gcgcctggtc gtgtgtgcgc cgaggtcgtg gcgccctacc cacccggcat
     2941 acccatgctg tatccgggcg aggtaatcga ccagagccat ctggagatca tccgtgaggc
     3001 aatgtcactc ggagcgattt tcagaggcct ggcaaccgac cctgacggag atctcgtcgt
     3061 cactgtggtg aaagctggga agagttgatg tcgaaaggcg tgttcatcac cttagaaggc
     3121 ccggacggct gcggcaagag cacccaatct cgtatgctgt gcgacgctct tgagaccgcg
     3181 ggttacgacg tagtgcgcac cttcgagccg ggcgggaccg caatcggtcg ccggatacgc
     3241 gagattattc tggataccaa gtccggcggg atgtgcggta tcactgagtt tctgctgtac
     3301 gcggcagacc gggcgcagga cgtgcatgag gtgataatgc cggcgttggc tgcgggacgg
     3361 attgtcatag gcgataggtt tttcgattcc agcattgcct atcaaggcta cgggctgggt
     3421 atggatgtag aggccgtgcg tgctgtgaac gcaatcgcga cgcgcggcct cattcccgac
     3481 ctgaccgtgc tgcttgatat cgacccggcc acgggcgttg cccgcgccac gggcggtcga
     3541 gacgccgaca gaatcgaaag gcgtaagctc gcattccaca catcggtacg caacgcgtat
     3601 ctgcaaatgg ccgcgaacga accggacagg ttcagagtgg tcggggtggg tgatctttct
     3661 atagaccagg tccacgcggc cgtattccgc gaagtcactg agtttctgac aaaccgggtg
     3721 agctcggcgg tcggccaata tcggccgggt gcgcgggagg tgtaggctga tgcatctcgc
     3781 cgaaatactc ggccaagatg acgcaatgcg catcctcgac gcggaaatcc ggtcgggcac
     3841 gatgtcccat gcatacctgc tgtggggccc acgtggaagc ggacgcaccc taatggcacg
     3901 caggctagcg ttggctgcca actgctcgct tcgtgaccag gcgccgccca agtcacttct
     3961 ttggtactgc ggcgagtgcg ccaattgcac gaagatcgtg agcggcgtgc atccggattt
     4021 ctcggtcatt gcgccatccg gtagcagcat caagatcgct cagatcaggg agatgcaatg
     4081 gcgaatatcg ctcaaggcca acgaatcaga gcttaagaca tggctgatag atgatgctga
     4141 ccagatgact gaagacgcgc aaaactgctt gctcaaggtg cttgaagagc ccccgggccg
     4201 ttcgctcata atcctggtcg cgcaggatcc acagatgatg ctgccgacga ttgcatcccg
     4261 ctgtcacggc ataaggctgg ggactgtgga taccggcgcc atgctggagt gggcaggccg
     4321 cgaacttggc ctgccgcctg agcgcgctcg atttctggcg gcgttgtcgc aaggtctgcc
     4381 cgggcgcctg gccagtctgg catcggatca cgactacttc gccttgaggg actcaataat
     4441 atcaaaggtg cgcactgtga tgtggtgcgg caacgggggg cttgcgctcg acacctcaga
     4501 aggtctgctg gatgaactga agaaagcctc tgcgcgagcg caggatgcgg atgctttcgg
     4561 ggaagcggtg ccccgggagg tgctggagat agccaccccg actggggcat gcgactgtgt
     4621 agccgggtgg ttccgagatc tattcgttct cagcctgacc ggagatgaag agcttgttat
     4681 caaccgggat tacatggagg cgctcgaaga ggatgcggcc catggttcac ctgctctatt
     4741 tggcaagtgg actattcgcg tgatgcagac cgcccgcgct ctcgaagcca acgcgagccc
     4801 caggctagcc atggacgacc tcatgctttg cttggctttc gcgcgatgaa cgcaatgaaa
     4861 aggtggccag attatggtct gtgttgttgg tgtaaggttt aagaaggcag gtaaaatcta
     4921 ctacttcgac cccggtgagc acgcgcttgc tccgggcatt ggcgtgatag tggagacggc
     4981 acgcggcacc gagtacggcg aggttgttgt ggggcccaag ctcgtgcctg aggacgatat
     5041 cgttcaacct ctcaaacagg tgctcagggc ggccacgtcc gacgataggc accaagtgga
     5101 ggagaatcag aagaaggcca agagggcttt tgacattggc cttgcgaaaa tcgcggcaca
     5161 caacctgccc atgaagctgg tagatgtcga gtacaccttt gatagcagca aggtgatctt
     5221 ctacttcacc gctgaaggac gggtcgattt cagagaacta gtccgcgacc tagcgtcggt
     5281 cttccgcact cgaatcgagc tgaggcagat cggtgtgagg gatgaggcca agatgctcgg
     5341 tgggatgggg ccgtgcggac gagctttgtg ctgcgcctcc tttctcggcg acttcgaacc
     5401 ggtgtcgata cgcatggcca aggagcagaa cctgtcgctg aatcctacca agatctctgg
     5461 aatatgtggc aggctgatgt gttgcctcaa gtacgaggct
//
LOCUS       DAOTAC010000170         5483 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_12492_length_5483_cov_4.794952, whole genome shotgun sequence.
ACCESSION   DAOTAC010000170 DAOTAC010000000
VERSION     DAOTAC010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5483
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12492_length_5483_cov_4.794952"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /gene="yedE"
                     /locus_tag="PLM74_07900"
     CDS             <1..615
                     /gene="yedE"
                     /locus_tag="PLM74_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YedE family putative selenium transporter"
                     /protein_id="HON43033.1"
                     /translation="VLPIVSAFLLILVIAAPAFIFASAKGPGSAHAPVLWALVAGLAV
                     GALAQRSRLCLAGGIRDLILIRDPHLFNGFAAIFVVSLILNLISGRFKLGFEGQPVAH
                     TDGLWNFASMAAVGLGAALLGGCPLRQCILAGEGDADGVMAFLGMLVGAAFAHNWGIA
                     SSAAGPTANGKIATIVALVVMAIIGYTGVAVSRRPDHQRTAREA"
     gene            620..901
                     /locus_tag="PLM74_07905"
     CDS             620..901
                     /locus_tag="PLM74_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010293608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HON43034.1"
                     /translation="MTHRVDARGLSCPQPVVMTKRVLEKTRLGMKAKAPGEHEASDAS
                     GTPEVIEILVDNRTAMENVSRFARYSGCDVTIDNGDDNDCYVIRVCVKG"
     gene            924..1253
                     /locus_tag="PLM74_07910"
     CDS             924..1253
                     /locus_tag="PLM74_07910"
                     /inference="COORDINATES: protein motif:HMM:NF023251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HON43035.1"
                     /translation="MADRSVDDHGAVSGLAPVFLISFPNVHHAFKCENVMRKPGRRIQ
                     VMPVPRQISSSCGLAVEVQAEDIVYGRDVASELERGGVDYEGIFLLRQDGRYELIGDC
                     MEEKRGQ"
     gene            1287..3086
                     /gene="pepF"
                     /locus_tag="PLM74_07915"
     CDS             1287..3086
                     /gene="pepF"
                     /locus_tag="PLM74_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019423988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HON43036.1"
                     /translation="MTKRLARADVPTEETWRLEDLFPTADDWRNELDAAVAHIPSVSA
                     FRGRLAEGAVVLLECLEATEQLMMRMLRVGTYAFLRFSEDGSNPVNQGMMGRVSSAQA
                     QVGAAIAFLQPEILALPDGTIERYLQEEPRLSPFRRHLEQVLSNKPYVLSHETEAALA
                     ALGEVFAAPSNIYGLSKSGDMSFDSVRDSKGQETPVSFATYEETLELSPDTVLRRSAY
                     ASFSEGLKRYQNTFGGTFGVEVKKNVAMARTRRFKSATHMFLHEQEVSEEFYHNLLRT
                     IQAGLAPHMRRYVELRKKVLGLDEVMYCDIEAPLDPGFRPGITYEEGSRLILDSVEIM
                     GAEYAQIVRAALQDRWIDRVDNIGKSTGGFCAPVYDVHPYVLTTWGGNMRSVFTIAHE
                     LGHSVHMVLSSRNQRLINTDMSRVFVEAPSTFNELLLSRCILGGQCDGQMRRWVLMQM
                     LATYYHNFVRHMLEAELTKRVYDLAEKGNPVTAALLSQLKGEILQEYWGGAVRIDDGA
                     RLTWMRQPHYYMGLYSYTYSAGLVTATVVAENLAKDGQAVAEQWVRVLKAGSTRKPLD
                     LARMAGVDLEDEATVRRATDYVGRVVDELVQSF"
     gene            3186..4784
                     /gene="murJ"
                     /locus_tag="PLM74_07920"
     CDS             3186..4784
                     /gene="murJ"
                     /locus_tag="PLM74_07920"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HON43037.1"
                     /translation="MADKSNGSLARFAGIVALGTVVSKVLGFLRETSFAAQFGASYAT
                     DAYLVAMIIPTLMLMGVGPAVTTTLVPVFADLERRRGRDAAFASVSAIINACFIISSV
                     LMVAGMVFARPVVRLVAPGFSGEAFALTVELTAILFPIAVLTVMAHCVTGVLHAVGRF
                     AAPAITGVVQNIIIITAIMVFGPQYGIRAVAVGTVLGAFSMLAVQWPALRACGYRHRF
                     TLDWNDRGLHQVGRLIGPIIVGTAASQAGSLVIRALASRLPEGSITYLNYSQRLAALP
                     VGVFGTALITVLYPALSRLYSDDKSEFSRTFRRSVGIVFFILLPMAAGLMILAAPVVR
                     LAFERGVFTAEATAATSVALVYAAIGIPFTGLADLASKAFYATHDTLTPVFVNVGAVG
                     VNVALSLALVGSMQHAGLSLAASCQPIASFIILQAILRRRAAATQSETRPSAGGEYSL
                     AASLAKSVVAAAVMWAAVSAFDPWFAARLPQSGTMAQAVRLAASVGVGVVIYFGMAAV
                     LRSEELSFAAGAVRSRLDRGKRAR"
     gene            complement(4897..>5483)
                     /locus_tag="PLM74_07925"
     CDS             complement(4897..>5483)
                     /locus_tag="PLM74_07925"
                     /inference="COORDINATES: protein motif:HMM:NF025099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbon starvation CstA 5TM domain-containing
                     protein"
                     /protein_id="HON43038.1"
                     /translation="NYAKVPTLPTFAEGGARIVETLGISRAMALNILAVFMVSFASTT
                     IDTATRLQRYVISEFGDSIGQKWLDNKYIAAGFAVVSAYLMAFRGSANVIWPLFGATN
                     QLLAGLALLTITVYLAENNKPTQYTMIPMIFMVFMTTWGMVANFRNYVAAGNTLLAVI
                     DGIIIVLEAWMIVEAITVLGRVRQGRLGQPNVKA"
BASE COUNT         1041 a         1491 c         1765 g         1186 t
ORIGIN      
        1 gtgctcccta tagtcagtgc atttctgctg attctggtca tagcagcgcc ggcattcatc
       61 ttcgcaagcg ccaaaggccc gggttcggca cacgctcctg tgctctgggc gctggttgcc
      121 ggcctcgcgg tgggagccct tgctcagcgt tcgcggctgt gcctcgcggg cggaatacgc
      181 gatctgatcc tcatccgcga tccccatctg ttcaatgggt ttgcggctat cttcgtggtc
      241 tcgcttatcc tcaacttgat ctccggtagg ttcaagctgg gatttgaggg tcagcccgtg
      301 gctcataccg atggtctttg gaactttgcg agcatggcgg cagtggggct gggagctgcg
      361 ctcctcggag ggtgcccgtt gcgccagtgc atcctagcag gtgaaggcga tgcagacgga
      421 gtcatggcat tcctcggcat gcttgtcggc gccgcgttcg cacacaactg gggcatagcc
      481 tcgtcagcgg cgggccctac tgccaacggg aaaatcgcta ccattgtcgc tctggtggtg
      541 atggccataa tcgggtacac gggagtagcg gtgtccaggc gaccggatca ccaacgaact
      601 gccagggagg cgtgacatca tgactcatcg tgtagacgcg cgcgggcttt catgcccaca
      661 gcctgtagtg atgaccaaga gggttctaga aaaaacgaga cttgggatga aagccaaggc
      721 gccgggagaa catgaggcct ctgatgcttc gggaacccct gaggttatag aaatccttgt
      781 agacaaccgt acagccatgg agaacgtatc gcggtttgcc cgctatagcg gctgcgatgt
      841 gaccatagat aacggcgatg acaacgattg ttacgtcatt cgcgtctgcg taaagggata
      901 agaatagcat ggatgatcgt agcatggctg atcgtagtgt ggatgatcat ggtgcggtat
      961 ctgggctcgc gcctgtgttt ctgatctcct ttcccaacgt tcaccatgcg ttcaaatgcg
     1021 aaaacgtaat gcgcaagccg ggacgtcgaa tccaggtgat gccggttccg aggcagatca
     1081 gttccagctg tgggctggcc gtagaggtgc aggccgagga tatcgtatac ggtcgcgatg
     1141 tcgcttcgga gctggaacgc ggcggtgtgg actacgaggg gatctttctg ctccgacagg
     1201 atggtcggta tgaactgata ggcgactgta tggaggaaaa gcgcgggcag tgatgaacag
     1261 aataccactg gaaaggggag ggtggtatga cgaagagact tgcccgcgcg gacgtgccga
     1321 cggaagaaac atggagactg gaggatctat tccctacagc cgacgactgg cgaaacgagc
     1381 tcgatgctgc ggtggcccat atcccttcgg tatctgcgtt tcgcgggcgg cttgccgaag
     1441 gggcagtagt acttctcgag tgccttgagg ccacggaaca gctgatgatg cggatgctcc
     1501 gagtgggcac gtatgccttt cttcgtttct ctgaggatgg atccaatcct gttaaccagg
     1561 gaatgatggg acgggtgtcg tcggcccagg cacaggtagg ggctgcgatt gccttcctgc
     1621 agcctgagat attggcgctc cctgacggca caatagagcg atatctgcaa gaggaaccgc
     1681 gcctttcgcc attccggcga catttggaac aggtcttgtc gaacaagcct tacgtgctgt
     1741 ctcacgagac tgaggcggcc ctggcagctc ttggcgaagt attcgccgcg ccgagtaaca
     1801 tctatgggct tagcaagagc ggcgatatgt cctttgattc cgtgcgcgac tcgaaagggc
     1861 aggagacgcc ggtgtcgttc gcgacgtatg aggagactct ggagctatcg cctgacactg
     1921 ttcttcgccg cagtgcgtat gcgtcattct cagaaggtct gaagcgttat cagaacactt
     1981 ttggcggcac ttttggggtc gaagtcaaga agaacgtggc catggcgaga accaggaggt
     2041 tcaagtcggc cacccacatg ttcctgcacg agcaggaggt ttcggaggag ttctaccaca
     2101 acctgctgcg aacgatccag gctgggctgg ccccgcatat gcgccgctat gtggaattgc
     2161 gcaagaaggt cttgggacta gacgaggtga tgtactgcga tatcgaggcc cctctcgacc
     2221 ctggctttcg gccgggcatc acgtatgaag aggggtcccg gctcatcctt gactctgtcg
     2281 agatcatggg cgcggagtac gctcagatag tgcgggcggc tctgcaggac agatggatcg
     2341 acagggtcga taacatcggc aagtccacag gcgggttctg cgcgccggtg tatgatgtgc
     2401 atccatacgt cctcaccaca tggggcggga acatgcgatc cgtattcacc atcgcgcacg
     2461 agctgggcca ctcggtgcat atggtgttat cctcgcgcaa ccagcggctc atcaacaccg
     2521 atatgtcgcg ggtatttgtc gaggcgcctt ccacgttcaa cgaactcctt ctgtcgcgct
     2581 gcatcctggg tgggcagtgt gatggccaga tgcgacgttg ggtgctcatg cagatgctgg
     2641 ccacctacta ccacaacttc gtccggcata tgctcgaagc cgagctcacg aagagggtat
     2701 atgaccttgc cgagaagggc aacccggtca ccgcggcgct cctgtcgcag cttaaggggg
     2761 agattctgca ggaatactgg ggcggagctg tgagaattga tgacggcgcc cgccttacct
     2821 ggatgcgaca gccccactac tacatgggtc tttattccta cacttactcc gccgggctgg
     2881 tgactgccac agtagtcgct gagaacctgg ccaaggatgg tcaagccgtg gccgagcagt
     2941 gggtccgggt cctaaaagcc ggcagcacca gaaagccgct ggacctggct agaatggccg
     3001 gagtggactt ggaggacgag gcgactgtgc ggcgcgcgac ggactatgta ggccgcgtcg
     3061 tagacgaact cgtgcagagc ttttgatcga agttgcagcc tggctgtggt aaactggtgc
     3121 ttgccgggta atggtcgggt gacggcattt gattgggccc ggcgtgaaat ggagaagtgt
     3181 atgctgtggc agacaagtcc aatggcagtc tcgctaggtt cgcgggcata gtcgcgctag
     3241 gtacagttgt gagcaaagtc cttggctttc tgcgggagac gtcgttcgcc gcccagttcg
     3301 gcgcgagcta cgcaacagat gcctacctgg tggccatgat tatcccgacg ttgatgctga
     3361 tgggagtggg gcccgctgtc accacgacgt tggtgccggt cttcgcagac cttgagcggc
     3421 ggcgcggccg agacgcggca tttgcttcgg taagcgcgat cataaacgca tgcttcatca
     3481 tctcgagcgt gctcatggtt gccggcatgg tgttcgcacg cccagtggtg cggcttgtgg
     3541 cgcccgggtt ctccggcgag gcattcgcgc ttaccgtgga acttaccgcc atcttgtttc
     3601 cgattgcggt tctgacagtg atggcacatt gtgtaacagg cgtcttacac gccgtgggca
     3661 ggtttgcggc gccggccatc accggagttg tacagaacat catcatcatc actgcgataa
     3721 tggtcttcgg cccccaatat ggcatccggg ccgtggccgt cggcactgtg ttgggcgcct
     3781 tcagtatgct ggccgtgcag tggccggccc ttcgcgcgtg tggctacagg caccgtttta
     3841 ctctggactg gaacgacaga gggttgcacc aagtgggccg gctgatcggg ccgatcatag
     3901 tgggtacggc ggcctcgcaa gcaggtagcc tagtcattcg ggccttggcg tcgcgcctgc
     3961 ctgagggctc gattacgtat ctcaactact cgcagcggct ggcggcgttg ccggtgggcg
     4021 tgtttggaac agctctgata accgtcctct acccggcact ctcccgcctc tactccgacg
     4081 ataagtccga attctcaaga accttcagac gttcagtcgg aatcgtcttc ttcatactgc
     4141 tgcctatggc ggcgggactg atgatcctgg ctgcgcctgt agtaaggctg gcgtttgagc
     4201 ggggcgtctt cacggctgag gccactgccg cgactagtgt tgcgctggtg tacgcagcca
     4261 tcgggatacc cttcacgggc cttgcggatc tcgcgtccaa agccttctac gccacgcacg
     4321 acacgctgac tccggtgttc gtgaacgtag gcgccgtagg agtcaacgtc gcgctcagcc
     4381 tagcccttgt gggaagtatg cagcatgccg ggctttcgct tgcggcatcc tgccagccca
     4441 tagcttcctt cataatccta caggccatct tgaggcgaag ggcggcggcg acccaaagcg
     4501 agacgaggcc ctcggcgggc ggggagtata gcctggccgc atcgcttgcc aagtcggtag
     4561 tggcggcggc cgtaatgtgg gcggcggtgt cggcgttcga cccgtggttc gcggcacgtc
     4621 ttccgcagtc gggcacaatg gcgcaggctg tgcggttggc tgcctctgta ggcgtgggcg
     4681 tagtgatata cttcggcatg gctgccgtgc ttagaagcga agaactatcg tttgccgcgg
     4741 gcgcagtgcg aagtcggctg gatcgcggca agcgggcgcg ctgaggcggc gggcagaaat
     4801 cagtagaaga agtgaaggag cggcgccccg accgcgtcgt gttgggccgc cgctcccgat
     4861 cgctgattca tgaggcccag cgtactagga aggttactag gcttttacgt tgggttgacc
     4921 gagccgccct tgcctcactc ttccaagcac ggttatggct tctacgatca tccacgcctc
     4981 aagcacgata atgatcccgt ctatcacagc caggagcgtg ttacctgcgg ctacgtagtt
     5041 gcggaagttg gcgaccatgc cccaggtggt catgaacacc atgaatatca tagggatcat
     5101 ggtgtactgg gtgggcttgt tgttctccgc taggtacacg gtgatcgtca gcagtgccag
     5161 ccctgcaagc aactgattgg tggcgccgaa cagcggccat atcacgttgg cgctgccgcg
     5221 gaaggccatc aagtaggcgc tgacgactgc gaaccccgcg gcaatgtatt tgttgtcgag
     5281 ccacttctgg ccgatgctgt cgccgaactc agagatcacg taccgctgca ggcgcgtggc
     5341 ggtgtctatt gtggtgctgg cgaagctgac catgaagact gccaggatgt tgagggccat
     5401 cgccctggag atgcccaatg tctcgacgat cctcgcgcct ccctcggcga acgtaggaag
     5461 ggtgggcact ttagcgtagt tgg
//
LOCUS       DAOTAC010000171         5476 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_12521_length_5476_cov_3.946135, whole genome shotgun sequence.
ACCESSION   DAOTAC010000171 DAOTAC010000000
VERSION     DAOTAC010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5476
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12521_length_5476_cov_3.946135"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="PLM74_07930"
     CDS             <1..355
                     /locus_tag="PLM74_07930"
                     /inference="COORDINATES: protein motif:HMM:NF013633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HON43039.1"
                     /translation="EERDPAEVSYPGQVAKTIRATVASMRLDAVAGAGFPASRTRLAQ
                     EIRVGRFRINGATCDSPAAHVAQGDVIICRGRGRIVVAEVAGATKRGRISLVLTRYSV
                     QMTDRREDSEDAYAT"
     gene            339..836
                     /locus_tag="PLM74_07935"
     CDS             339..836
                     /locus_tag="PLM74_07935"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HON43040.1"
                     /translation="MLTPLDVHSKQFTARFGRYSAAEVDDFLDLVGQSYDQIYRENAE
                     LKERLRSLAEKPQQPESADVGETLKQTLLMAQKAAEDARRNAEEKARLILENAEREAA
                     AVLDRAQEKVRSQEQRLEDLVAQHGTLRARMKAMLEAYLEMLEDSALNLSDAKPGSVR
                     DGRKE"
     gene            881..1219
                     /locus_tag="PLM74_07940"
     CDS             881..1219
                     /locus_tag="PLM74_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012447779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5665 domain-containing protein"
                     /protein_id="HON43041.1"
                     /translation="MHRKPRARASEHARARSRTGLSGNSLVPKLVELSRRLEESSIAE
                     YVEFMRNPRRVIWSNFLGGAARGFGFAVGASVLAAVFLYLLSAIISLNVPIIGKFVSE
                     IVKFVEEHRG"
     gene            1249..1815
                     /locus_tag="PLM74_07945"
     CDS             1249..1815
                     /locus_tag="PLM74_07945"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HON43042.1"
                     /translation="MDPRELGIYRKKLVRAKERAQSRITQLEETSGFDRPESDWAGEL
                     SSYDNHTADSSSALEMRSINLGLINHSEDRLAQIDAALTRVDQGVYGKCVQCGRDIPP
                     ERLDAIPWAVSCATCGVRQPSPAGLGEARPIEEEVIQPPFGGVDPARAYDDPGVHGED
                     IWDALASYGNANSPQDDEAGHSGDVDSH"
     gene            1904..2350
                     /gene="lspA"
                     /locus_tag="PLM74_07950"
     CDS             1904..2350
                     /gene="lspA"
                     /locus_tag="PLM74_07950"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HON43043.1"
                     /translation="MALFLVSAWVMLLDQVSKSVVARLVPMYDTVACLGGLLRITHVR
                     NSGAAFGVFQGRQPLLIAAALAAAVIVVVFYPRVSVRNWPARFGLALGLGGALGNLID
                     RLRFGGVVDFLEVGPWPVFNLADTAIVVGVALILLAMMRSPGRKEE"
     gene            2354..3139
                     /gene="lgt"
                     /locus_tag="PLM74_07955"
     CDS             2354..3139
                     /gene="lgt"
                     /locus_tag="PLM74_07955"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HON43044.1"
                     /translation="MHPVLFNLWKYPVYSWGLSLAVAFVVGTVYAAWIGKKKGVDPDN
                     VIDLALFVCIASIVGARLLYVMLNIRSYVDYPMSVFYMRDGGLSYFGGLGAGIGVGVL
                     YCRRQKISIGKIADIVAPAITIGYAIVRVGCLLNGCCYGRISEVPWALAAGPGDVLRH
                     PTQLYAAISSVVIFFLLKAAEPKKKFEGQILWLYVGLYSVARFIVEFWRVGTRDYFAP
                     FTATQILCVLLAVLAFGAIRVKARTAKPIGTGMTGAEGPSARA"
     gene            3143..4087
                     /locus_tag="PLM74_07960"
     CDS             3143..4087
                     /locus_tag="PLM74_07960"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009645639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HON43045.1"
                     /translation="MDVVVSSPAHADAGLRLDVYLAKEQIAPSRSFVQRLIEDRAVRV
                     NGRIEAKPSYRVRAGDAIEVSLRKPDAEPACVPPENIAIAVLYEDDDVLVIDKPRGMT
                     VHPGAGRTTGTLVNALLGRQTSLSGIGGPLRPGIVHRLDRNTTGVMIVAKNDRAHLRL
                     AADLAAHRVGRHYLALVRGNIVENEGVVDAPIGRHPVDRKRQAVVVSGRRAVTRFRVL
                     ARFGDYTLIEATLETGRTHQIRVHMQYIGRPVAGDPVYGGVRDELGLAAQALHSARVE
                     FTHPATGEHMVFAAGMPGDMRAAVEALRAREQGRTAGR"
     gene            4084..5262
                     /locus_tag="PLM74_07965"
     CDS             4084..5262
                     /locus_tag="PLM74_07965"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HON43046.1"
                     /translation="MRGALRSLGVALLCIAAVAAGSPCGGAAGAGADSGKLEVSEHAV
                     RLLAQVARQPRLTLAAAGDCMFDRGVRAVCDERGRDWPLSAVRGILTGADVAFLNLET
                     SIARGGARLPGKGIWFRSAPEFAQELAAAGVDVVTLANNHVLDYDDPAFMETLSNLAA
                     AGIAVCGGGKNMAEARRPAVLSANGISIAFLGYSEFAHIYWSVASPKRFVASEASPGV
                     SPWERKSIMEDIRHAKRLADHVVVSFHWGDEYVSMPADRQVQLAHAAIDAGASVVHGH
                     HPHVLQPVEVYHGGVIFYSLGNFVFDQKKPATVESMIAHVTFSPEAVVRAEIIPVRIE
                     ECRPRPLAAWAARAAVDRISLASARMGARIVTIGERGLVMGPIKGSPIEAEGFRNLLP
                     "
     gene            complement(5197..>5476)
                     /locus_tag="PLM74_07970"
     CDS             complement(5197..>5476)
                     /locus_tag="PLM74_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43047.1"
                     /translation="GSHVVLRRRAGEDVPEETIRAAALLAAYYSRARMSSNVAVDCTE
                     ARNVRKPRGARPGMVIYDRHKTIWVTPSREEIAEAFGLNGAALDRAHD"
BASE COUNT          991 a         1561 c         1833 g         1091 t
ORIGIN      
        1 agaagagcgg gatccggcgg aggtttcgta tccggggcag gtcgcaaaga ccattcgcgc
       61 tacggtggct tccatgcgtc ttgacgcagt ggccggagcg ggcttcccgg cgtcgcgcac
      121 tcgtctggca caggagatcc gggtaggcag gtttcgtatc aacggagcaa cttgcgattc
      181 gccggcagca cacgttgcac aaggagatgt gattatctgt cgagggcgcg gcaggattgt
      241 cgttgcggag gtcgcaggcg ccacaaaacg tgggcggatc tctttggttc ttacgcgtta
      301 ttctgtgcag atgactgacc gtagggagga tagtgaagat gcttacgcca cttgatgtgc
      361 attcgaagca gttcacagcc aggtttgggc gatatagcgc cgccgaggtg gacgactttc
      421 tggaccttgt gggccagagc tacgaccaga tctaccggga gaacgccgag ctcaaggaga
      481 ggctccgttc gcttgccgag aaaccgcagc aacctgaatc ggccgacgtc ggtgagacgc
      541 ttaagcagac cttattaatg gcgcagaagg ctgcggagga cgcccggcgg aacgctgagg
      601 agaaggcccg gctgattttg gagaatgccg agcgtgaagc ggctgccgtt ctcgatcgcg
      661 ctcaggagaa ggttcgcagt caagaacagc gcctggagga tctcgtggct cagcacggca
      721 cgctcagggc ccgcatgaag gccatgcttg aggcgtacct ggagatgctt gaagattccg
      781 cactgaacct atctgatgcg aaaccaggct ctgttcgcga cggtcgtaag gagtaaaacc
      841 cgctgcgtgc gggcagtcta caccatgcga ggtgtagcgc ttgcacagga aacctcgggc
      901 cagagccagt gaacatgccc gtgcccgctc taggacaggc ctttcgggga actcccttgt
      961 gcccaagctc gttgaactct ctagaaggct cgaagagtcc agcatcgcgg agtacgtcga
     1021 gtttatgcga aacccccggc gcgtcatctg gtcaaacttc ctaggtgggg cggcgcgggg
     1081 gttcggcttt gcggtcgggg catcggtgct cgctgctgta tttctctacc ttctgtcggc
     1141 catcatctcg ctcaatgtcc cgataatcgg caagttcgtg tccgagattg tgaagttcgt
     1201 cgaggagcac cggggctgag tatacgaggc gaaggcggag gcggtaggat ggatcccaga
     1261 gagctgggaa tatataggaa gaaactcgtg agggccaagg agcgtgccca atcccggatc
     1321 acgcagctgg aagagacttc cggcttcgac cgccccgaat ccgattgggc tggcgagcta
     1381 tcgagctacg ataaccacac agccgactcc agctctgcgc tggagatgcg ctctatcaat
     1441 ctgggcctga taaaccacag cgaggaccgg ctcgcgcaaa tcgacgcagc cttgacccgg
     1501 gtagatcagg gcgtttacgg aaagtgcgtc cagtgcggcc gagacatacc tccggagcgc
     1561 ctcgatgcca ttccatgggc tgtaagctgc gccacatgtg gggtcaggca gccctcgccc
     1621 gcgggcctcg gagaggccag gcctatcgag gaagaggtga tacagcctcc tttcggaggc
     1681 gtcgatccgg ccagggcata cgatgacccg ggggtgcatg gcgaggacat atgggacgcc
     1741 cttgcgtcct atggcaacgc caatagcccg caggacgacg aggccggtca ctccggagac
     1801 gtggattccc actgaacgct ggggcgccga cggcgtttac tgggcaatgc cggtgtgcta
     1861 aaatggatca cggactttgc tgcttgcgat ggagtggagg cttgtggcgc tgtttctggt
     1921 gtcggcctgg gttatgctgc tggatcaggt ttcgaaatcc gtcgtggcgc gactggttcc
     1981 aatgtacgac acagtcgcct gcttgggcgg gcttcttcgc ataacccacg tgcgcaatag
     2041 cggcgccgcg ttcggcgtct ttcagggcag gcaaccgctg ttgattgcgg ccgcgctggc
     2101 ggcggcggta attgtggttg tgttctatcc cagagtatcc gttaggaact ggccggcccg
     2161 gtttggcctc gccctggggc ttggaggcgc gctgggcaac ctcatcgaca ggctgcgttt
     2221 cgggggcgtt gttgattttc tggaagttgg gccatggccg gtgtttaacc tggccgacac
     2281 tgccattgtt gtgggggtcg cgctgattct actggcgatg atgcgcagcc cagggcggaa
     2341 ggaggaataa ttcatgcatc ctgtcctatt caacttgtgg aaatatcctg tctactcttg
     2401 ggggctttcg cttgccgttg cattcgtcgt cggaacggtg tatgccgcct ggattggaaa
     2461 gaagaagggc gtggaccccg ataacgtaat cgacctcgcg ctgtttgtgt gtattgcgtc
     2521 gatagttgga gctaggctgc tctacgtcat gctcaacatc agaagctatg ttgactaccc
     2581 catgagcgtg ttctacatgc gtgacggggg cctttcttac ttcggcgggc tgggcgccgg
     2641 catcggcgtc ggcgtgctgt actgccgcag gcagaagatc tccatcggca agatagcgga
     2701 tattgtcgcg cctgccatca ccatcggcta cgccatcgtg cgcgtcggtt gcctactcaa
     2761 cggatgctgc tatggccgca ttagcgaagt gccgtgggcg cttgccgccg gccctggcga
     2821 cgtactgcgc catcccacgc agctctacgc ggcgatctcc agcgtcgtaa tattcttcct
     2881 gctgaaggcg gccgagccga agaagaagtt cgagggccag atactctggc tgtatgtggg
     2941 cctctactct gtggcccggt tcatagttga gttctggcgg gtaggcacgc gcgactactt
     3001 cgcgcccttt actgccactc agatcctctg cgtgctcctg gcggtattgg cctttggcgc
     3061 gatccgggtc aaggcccgca cggcaaaacc gattgggacg ggcatgacgg gcgccgaggg
     3121 gccttcggcg cgggcgtagg acatggacgt ggtcgtctcg agccctgctc atgccgatgc
     3181 aggcttgagg ctagacgtat accttgcgaa ggagcagatt gctccttcgc gttcttttgt
     3241 ccagagactc atagaagaca gagcggttcg ggtcaacggt aggattgagg cgaaaccttc
     3301 ctaccgggtt cgggccggcg acgcgataga agtatcgctg cggaagcctg atgccgagcc
     3361 ggcatgcgtg cctcccgaga atatcgccat cgccgtgctc tacgaagatg atgatgtgct
     3421 ggtaattgac aaacctcgcg gcatgactgt ccatccgggg gcgggccgaa caaccggaac
     3481 actggtcaat gccctcctgg gcaggcagac gagtctgtcc ggtatcgggg gtccgctcag
     3541 gccgggcatt gttcaccgtc tggaccgcaa tactacgggc gtgatgatcg tagcgaagaa
     3601 tgaccgggct cacctaaggc ttgcggcgga cctggccgcc caccgcgtgg gccgccacta
     3661 cctggcgctg gtgcgaggca atatcgttga gaatgaaggg gtagtggacg ctcctatcgg
     3721 tcgtcaccct gtggacagga agcgccaggc agtcgtggtc agcggcaggc gggctgttac
     3781 gcgctttcgc gtgctggcga gattcggtga ctacacgctg atcgaggcga cgctggaaac
     3841 gggccgaacg catcagataa gggtccatat gcagtacatc ggccgcccgg tggcgggcga
     3901 cccggtgtat ggcggggtcc gcgatgagct gggattggct gcccaggccc tccactctgc
     3961 gagggttgag ttcacacacc cggctacagg agaacacatg gtgtttgcgg ccggcatgcc
     4021 cggcgacatg cgcgccgctg tcgaggcgct gcgggcgcgg gaacaaggaa ggacggctgg
     4081 cagatgaggg gggcgctacg tagcctgggt gttgcgcttc tctgcattgc ggcggttgcg
     4141 gcagggagcc cctgcggcgg tgcggccggc gccggtgcgg actccggcaa gctcgaagtg
     4201 tcggagcacg ctgtgcgcct gcttgcccag gtcgcccggc aaccccgcct tacgctggcc
     4261 gcggcaggcg attgcatgtt tgatcggggc gttagggcgg tctgcgatga gcggggaagg
     4321 gactggccac tgtcggccgt gcgcggcatt ctgacaggtg cagacgtggc ctttctgaac
     4381 ctagagacca gcattgcccg gggtggcgcc cgcctgccgg gcaagggcat atggtttcgc
     4441 tccgcccccg agttcgccca ggaactggct gctgcaggcg tcgacgtcgt cacattggcc
     4501 aataaccacg ttctcgacta tgacgacccg gcgtttatgg agaccctgtc aaacctggcg
     4561 gctgcgggga tcgcggtgtg cggaggcggg aagaacatgg ccgaagcgcg ccggcctgca
     4621 gtgctgtccg ccaatggcat atccattgcc ttcctgggct attctgagtt cgcccacatc
     4681 tactggtcag tcgcgagtcc caagcggttc gtggcaagcg aggccagccc gggcgtttcg
     4741 ccgtgggagc gcaagtctat catggaggac atcaggcacg ccaagcgtct ggcagatcat
     4801 gtagttgtct cattccactg gggagacgag tacgtgtcca tgccggccga caggcaggtt
     4861 cagctggcgc acgcggccat agacgccggc gccagtgtgg tgcacgggca ccatccccac
     4921 gtgcttcagc ctgtggaggt ctaccacggc ggagtgatct tctactcgct ggggaatttc
     4981 gtattcgacc agaagaaacc ggccaccgtg gaaagcatga tcgcccatgt gaccttctcc
     5041 cctgaggcag tggtgcgcgc cgagatcatc cccgtccgca tagaggagtg tcgccctcgg
     5101 cctctggcgg cctgggctgc ccgggccgct gtagacagaa tcagcctggc ttcggcacga
     5161 atgggcgccc gcatcgtgac tatcggggaa cgcgggctag tcatgggccc gatcaagggc
     5221 agccccattg aggccgaagg cttccgcaat ctcctcccgt gatggggtga cccagatggt
     5281 cttgtggcgg tcgtagataa ccatccccgg ccgcgctcca cgcggtttgc gcacattcct
     5341 ggcttcggta cagtccacgg ccacgttgga cgacatccgg gcgcggctgt agtaggccgc
     5401 gagcagtgcc gcggcgcgga tagtctcttc gggaacgtct tcgcctgccc gccgcctaag
     5461 caccacatgc gagccg
//
LOCUS       DAOTAC010000172         5438 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_12616_length_5438_cov_4.918819, whole genome shotgun sequence.
ACCESSION   DAOTAC010000172 DAOTAC010000000
VERSION     DAOTAC010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5438
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12616_length_5438_cov_4.918819"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="PLM74_07975"
     CDS             complement(<1..675)
                     /locus_tag="PLM74_07975"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HON43048.1"
                     /translation="MGISAQSTQFDKRKDLLGLKDVSNEEIELILNTALPMKDIIRRE
                     IKKVPTLRGKGIITVFYENSTRTRTSFELAGKYLSADTSNLAVSTSSVQKGETLRDTI
                     KTLEMMGPDILVMRHSMTGAPHYVARNTSMRVINAGDGTNEHPTQALLDMFSIRERKG
                     RIDNLKVAIVGDILHSRVARSNIYGLAKFGCEIRVAGPPTLIPPGLDRLGVKTCFNLE
                     DAIRGVD"
     gene            complement(809..1378)
                     /gene="pyrR"
                     /locus_tag="PLM74_07980"
     CDS             complement(809..1378)
                     /gene="pyrR"
                     /locus_tag="PLM74_07980"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HON43049.1"
                     /translation="MKERTVLVELRDKTQIMDEMGMKRALTRIAHEIIETNKGVQGVA
                     LVGIRRRGGPLAVRLADLIESFEGVRPPVGVLDITLYRDDLTALANQPLVHRTEVHFD
                     ITGMSIVLVDDVIYTGRTVRAGLDALIDLGRPKRIQLAVLIDRGHRELPIKPDFVGKN
                     VPTSREEVVSVCVLEIDDEDKVTIQERIQ"
     gene            complement(1707..1853)
                     /locus_tag="PLM74_07985"
     CDS             complement(1707..1853)
                     /locus_tag="PLM74_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43050.1"
                     /translation="MYWCVLMSGTKPSFEVVSLLELLELLLVGKEVGRAVMLFALHSA
                     VLPA"
     gene            2950..3963
                     /locus_tag="PLM74_07990"
     CDS             2950..3963
                     /locus_tag="PLM74_07990"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HON43051.1"
                     /translation="MERLAEQNRVHQDGAEAPEANGPKAMDYVEMSLPELRALDPGKT
                     VFMMSVSPIEVHGPHLPVGTDVFVSDNLAERYMAELHKRQPHLQFVRLPSLYIGSDAL
                     PVAGSLSVPAVHLEGVLKAYGKGLAKQGFRYLFVADNHGGPRHQMAVEAAARALWRKY
                     RFYLIDPFGADFRYMVQHDAEFMSRTGLGPGACGDDPDSHAGTNETSLMLACSPQRVQ
                     EDCRTVRASLPPAKRGAAKLVGGLATVVRLLRGRTLGRDLDHLANTLAWVSDPEMIPY
                     MGDPSKATREAGEAMLAARVEVAMDLFERALAGGTEPDSKREPAHITPMLWGLRIMRK
                     LPE"
     gene            4054..>5438
                     /locus_tag="PLM74_07995"
     CDS             4054..>5438
                     /locus_tag="PLM74_07995"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HON43052.1"
                     /translation="MHIRFLGGAGEVGASAILVTSNSGRNILLDVGVRVNEEGTEMLP
                     NLEPLNSVSLDAIIMSHAHLDHSGALPLVAKVSPGAGIYATAATKDLAKVLLYDSIKV
                     AEFREGLQLFDADDAEQALDRMVTYGFEAAFEPAPGVLAMFLPAGHILGAAAVLLQTE
                     EGTLLYTGDFTSFEQETVGMQRFTGMLKRGVDVAVVEATYGSRIHAPRSHELRRLLDA
                     IGEVVSDGGRVLIPAFAVGRAQELVLSLRNYIRRTKKKFPVYVDGLIRNVNAVFAHNP
                     RYLADRYRKEALRGEELFYTNGIESVTTKAQRDKIIASGEPCVIIASSGMLTGGVSPV
                     YAERIVEGRKNLLAIVGYQDEESPGRRLLDLAGRTEGERVIRLGDAEYQVRCRVARFG
                     LSAHADSLGIAASIKPLAPRLVLVNHGNDESLEAMSEALAAELPEARVEVAVPGAAYE
                     YAADGSVIADGR"
BASE COUNT         1089 a         1485 c         1723 g         1141 t
ORIGIN      
        1 gtccacgccg cgaatagcgt cctcaaggtt gaagcaggtc ttgacgccta ggcgatcgag
       61 accgggaggg atcagcgtgg gcggcccggc aacgcggatc tcacagccga acttggctag
      121 cccgtagatg ttggacctgg caacacggct atggagtatg tctcccacaa tggcgacctt
      181 gagattgtcg atgcggcctt tgcgctcgcg gatgctaaac atgtccagca gcgcctgcgt
      241 ggggtgctca ttggtgccgt cgcctgcgtt gatgacacgc atcgaggtgt ttcttgccac
      301 gtagtgcggc gctccggtca tcgagtggcg catcaccaga atgtcggggc ccatcatctc
      361 aagcgtcttg atggtgtcgc ggagtgtttc gcctttctga acactgctgg ttgagacggc
      421 caggttggat gtatcggcgc tgaggtattt gccggcgagt tcgaaggagg tcctggtgcg
      481 cgtgctgttc tcgtagaaaa cagtgatgat gcccttgccg cggagggtgg gcactttctt
      541 gatctcacgc ctgatgatgt ctttcatggg gagtgcggta ttcagaatca gctcgatttc
      601 ttcgttgctc acatccttca gacccaagag gtctttacgc ttatcgaact gagtgctctg
      661 agcgctgatt cccattgctg cagcctcctt atccccgctg caggatggcc aggccgagcc
      721 agcgcgccac agggcgcgga gagactttcg gatcccttgc cagcctcacg ggactagttt
      781 aaagggacat gtttactcaa tcactggatc actggatgcg ttcctgtata gtcaccttgt
      841 cctcgtcgtc tatctcgagc acacacacgg acactacttc ctcacgggat gtcgggacat
      901 tcttgccgac gaagtccggc ttgattggca actcccgatg gcctcggtcg atcaggactg
      961 ctagctggat ccgtttgggc cggcccaggt cgatcaaggc gtcaagcccc gcccgcacgg
     1021 tgcgaccggt gtagatcacg tcatccacta gtacgatgct catgcccgtg atgtcgaagt
     1081 gaacctcagt ccggtggacc agtggctgat tagccagcgc agtcaggtcg tctcgataca
     1141 gagttatgtc cagaacacct acaggcggcc tgactccttc aaaagattcg atcaaatcgg
     1201 ccagtctgac agctagcggg ccgcccctgc gccttatgcc caccagcgct acaccctgca
     1261 cacctttgtt cgtttcgatg atctcatggg caatgcgggt gagggcacgc ttcatgccca
     1321 tctcgtccat gatttgagtc ttgtcacgaa gttccacgag caccgtcctc tctttcatgc
     1381 tgtcctggta tggtgatctg tcagttagtg tgcatgccgg tcagcaaaaa gcctgcccaa
     1441 gccgtaaggc cgggcaggca tcatgcacta aacccctgca ggacagccaa ttcgcccttg
     1501 tcggcctcac gggaccaact taaaggtgat gtcgaacaca atctggggat tccgggtgcg
     1561 ttgcgtggtt cattacctga caagagcata gaccatgttc gcgtgcgcgt caatacgtgt
     1621 ttcgccccat gacttcagat tattctaccg ttctcagcgc gtacagcgtt gcgcagggcg
     1681 aacaggacta cgcagggcga acagcactac gcagggagaa cagcactgtg cagggcgaac
     1741 agcattacgg cacgaccgac ctctttgccg accagaagca gttccagcaa ctccagaagc
     1801 gagacaacct caaagcttgg ctttgtgccc gacatcagta cacaccagta cattcacgta
     1861 gagatccggg tcggcggctt agcgtctgcc ggaatggatc tcaagatccc accgcaggtt
     1921 cctcgcagac tgagccggcc tgagagcctg ctcgttgcca ggcccgagga gttcggcact
     1981 ggctgcaagt cgtacatcga tagcgttact cgatctcgtg acgcggagtg atgcgagtgc
     2041 cggcctatgt cccaataaat ggagaacgca atttttgacg atactttttg agataccggc
     2101 agtttttgag tacatcgatt tgcgcttcag ttctgatcac ctaataaatg ccgttgtgca
     2161 gactggttct ggcgtatgct tacagtttgc ggaatcagca tagccgctgt gagaggaggc
     2221 gcctcaaaac gcatcacatt gggcgccagt aatacccttt atgatggcgg ctactactaa
     2281 ttccgcctac atggcgatta ctgcaggcgt attaacagtt tttagcctgg cggcgaaaac
     2341 gatagtgtca gattttgcgc ctatcggcaa aactattgcc aaaatccgag tacatccctg
     2401 ctagacgcca tgtttcagac aaaaggccag gcaaacccac agatatacgt cactagcccc
     2461 ttaggtcgcg taggttctat gggtccccga ggtctcgtag gtctcgtagg tctcgtagat
     2521 cccgtaggtc ggggggtccc ctatgtcccc gaggtcccgg aggtccatat gtcccgaagt
     2581 cccgaaggcc gaaatcccga aggccgagga ccctagatgt tgctcataga tcccaggggt
     2641 ctcggaaacc ccagaggtcg ccgacgggtg gtcgctgaag acgccccttc tgccgccacg
     2701 gcgcctggta atacctgccg ctctcttagc ggcatctggt gactggcgcc gcagccgagg
     2761 tcggggttgg catcgggttg ccggggctgg cgccgggtag ccgggcggcg caagcaagcg
     2821 gagtgagcgg aaggataggg ggcactttga gttgaaacac gatacagtgt cagtgcggcg
     2881 cggactgtcc agaaactaga aactgaccgt aagcagatca tcaaagagct ctgatggagg
     2941 tgattggtcg tggagagact ggccgagcaa aacagggtcc atcaggatgg ggctgaggcg
     3001 cctgaggcga atggccccaa agcaatggac tacgtggaga tgtctcttcc tgaactgcgc
     3061 gcattggatc cggggaaaac tgtcttcatg atgagtgtta gtcccattga ggtacatggc
     3121 ccgcatctgc cggtcgggac ggacgtgttc gtctcggata acctggcgga gcgctacatg
     3181 gccgagctac acaagaggca accgcatctt cagtttgtgc ggctcccttc gctgtatatc
     3241 ggctccgacg cgcttccggt ggctgggtcg ctgtcagtgc ctgcggtcca tcttgagggg
     3301 gtgcttaagg cctacggaaa gggcctggcc aaacagggct ttcgctacct gtttgtcgcc
     3361 gacaaccatg gcggcccacg ccatcagatg gctgtggagg ccgcggccag ggcgctctgg
     3421 aggaaatacc ggttctactt gatagaccca tttggcgccg acttccgcta catggtgcag
     3481 catgacgcgg agtttatgag caggaccggg ctggggccgg gggcctgtgg cgacgacccc
     3541 gacagccacg ccggcacgaa cgagacctct ctgatgctgg cgtgttcacc gcagcgtgtt
     3601 caggaggatt gtcgcactgt tagagcatct ctccctcctg ctaagagggg cgctgcgaag
     3661 ctagtgggtg gtctggctac ggttgtgcgg ctgctacgcg ggcgaacgct cgggcgcgat
     3721 cttgaccatc tggccaacac gctagcatgg gtgagcgatc cggagatgat cccgtacatg
     3781 ggtgacccga gcaaggcgac gcgcgaggcg ggcgaggcca tgcttgctgc acgcgtagag
     3841 gtggccatgg acctgttcga gcgggcgctg gcgggtggga ctgagccgga ttcgaagcgg
     3901 gagcctgccc acatcacgcc catgctgtgg gggctgcgga tcatgcggaa gctgccggag
     3961 tagggcgcag tcggaggccc gggcggagcg ttgcggtgag tcggagctgg gctcaagcct
     4021 gagcaggctg aatacacaca gggagaattg cgtatgcaca tacgatttct ggggggtgcc
     4081 ggtgaagtgg gcgcctcagc catactcgta acatccaact ccgggaggaa catactgctt
     4141 gacgtgggcg tgcgggtgaa cgaggaaggc actgagatgc tgcccaacct ggaaccgctg
     4201 aacagcgtga gcctggacgc cattatcatg tcccacgcgc acctcgacca cagcggcgcg
     4261 ctgcctctgg ttgcgaaggt gtcgccgggc gcgggcattt acgccacggc tgctaccaag
     4321 gatctggcta aagtgctgct atatgactct ataaaggttg cggagtttcg ggaggggctg
     4381 cagctttttg atgcagacga cgctgaacag gctcttgaca gaatggtgac ttacggtttt
     4441 gaggcagcgt tcgagcccgc tccgggcgtt ctggcaatgt ttctgcctgc cgggcacata
     4501 ctgggggctg cagctgttct actgcagacg gaggagggta ctctcctcta cacgggcgac
     4561 ttcaccagct tcgagcagga gactgtgggt atgcagcggt tcaccggcat gctgaagcgc
     4621 ggtgtggatg tggccgtggt ggaggcgact tacggctcgc ggatacatgc gccccgatca
     4681 cacgagctgc gtaggctgct cgatgccatc ggcgaagtag tgtcagacgg ggggagggta
     4741 ctgatccccg cgttcgccgt ggggcgggct caggaattgg tgctgtcgct gcggaactac
     4801 atcaggcgga cgaagaagaa gttcccggtg tatgtggacg ggctgattag gaatgtgaac
     4861 gcagtgttcg cgcataatcc gcgctatttg gccgacagat accgcaagga ggcgctgcga
     4921 ggggaagagc ttttctacac aaacgggata gagtcagtca ccacgaaagc gcagcgtgac
     4981 aagataatcg catctggcga accttgcgtg atcatcgcca gttccgggat gcttacaggc
     5041 ggcgtatcgc cggtatacgc agagcggatt gtggagggac ggaagaacct gctggccatc
     5101 gtgggctacc aggacgagga gtcgcccggc cgcagattgc tggacctggc cggccggacg
     5161 gagggcgaac gcgtgattcg gcttggtgac gctgaatacc aggtcagatg ccgcgtggcg
     5221 agattcgggc tttcagccca tgcggactca ctaggcattg cagcgtcgat caagccgctc
     5281 gctccacggc tcgtgctggt caatcatggc aatgacgaat cgcttgaggc aatgtcggag
     5341 gcattggcgg ctgagttgcc ggaagccagg gtggaggtgg ctgtgccggg ggcggcctat
     5401 gagtatgcag ccgatggcag tgtgatcgca gacggtcg
//
LOCUS       DAOTAC010000173         5375 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_12789_length_5375_cov_4.349436, whole genome shotgun sequence.
ACCESSION   DAOTAC010000173 DAOTAC010000000
VERSION     DAOTAC010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5375
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12789_length_5375_cov_4.349436"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1275)
                     /gene="pulA"
                     /locus_tag="PLM74_08000"
     CDS             complement(<1..1275)
                     /gene="pulA"
                     /locus_tag="PLM74_08000"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR02104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051060 - pullulanase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HON43053.1"
                     /translation="MGLEVCPVVAVGGQELFELYYPGDDLGAVYSPDSTAFRVFAPTA
                     LGVSVMLYSSAQSAAGWEVSMSPDCDGTWLAVVPGDLAGLCYIYRVFHGGCVKEAVDP
                     YARALTVNGGRGVVADLSSTDPEGWAGDERPPLAAATDAVVYEAHVRDFSISPDSGTQ
                     YRGKYLGMSERGTRGPYRVRTGVDHLVELGITHVHLLPIQDFASVDELSPDGYNWGYD
                     PFHFFVPEGSYSCDPESWATRITEVKQMIHALHRAGLRVVLDVVYNHTYSTEESPLQM
                     IVPGYYHRTDAAGMFTNGSGCGNELATERPMVRKLIVDSLKYWVQEYHVDGFRFDLMA
                     LIDCDTMREIGCELRAVEPSLLLYGEPWAGGPSGLCHTRMFTAGKQRSTGIGVFNDSF
                     RNAIKGDNDGSRPGFATGAAGCEREIAKGVVAS"
     gene            complement(1375..3189)
                     /gene="pepF"
                     /locus_tag="PLM74_08005"
     CDS             complement(1375..3189)
                     /gene="pepF"
                     /locus_tag="PLM74_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013401444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HON43054.1"
                     /translation="MAETVEAIPERKDIPEELRWKVEDIYASDQACEADMSAVRNKIP
                     DMASYAGRLGAGPKALLECLKLRDEVDYLVTKAYTYSRMRKDEDNANADYQSMHDRAA
                     SLAVEAASATSFLRPEILAISDDELERFMSEEPGLELYRMFLDNIRRMRDHTLSASEE
                     RIMAMAGEIGHAVPAIFTMLNNADLKFPSIVDADGNTVEVTHGRYIRFMQSRDRRVRK
                     DAFDALYSSYRALINTVAATYSASVKADSFNARVRGYSSAREAALDENNIPVEAYDNL
                     VSAVRSRVDLMHRYVRLRKKALDLPEIHMYDVYAPVVPDVEWPVTYDEAKAMVRTGLA
                     PLGGEYIRALSAGMESGWIDVMENRGKTSGAYSWGVYGVHPYVLLNWQDNLSNAFTLA
                     HEMGHSMHSYYTWAKQPYVYSDYLIFVAEVASTVNEVLLTRYLLDRTDDPARRMYLLN
                     HFLEEFRGTVFRQTMFAEFEKITHEMHESGQALTPDALNQVYLRLNRDYFGPDMVLDD
                     AIAYEWARIPHFYRAYYVYQYSTGFSAAVALADSILDQGQPAQDRYLEFLSGGNARYP
                     IDLLKRAGVDMTSPEPVLAALGVFEKTLDEMETFFEGK"
     gene            complement(3214..4626)
                     /locus_tag="PLM74_08010"
     CDS             complement(3214..4626)
                     /locus_tag="PLM74_08010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HON43055.1"
                     /translation="MEHVRTTDFTTGSIPRHLIAFATPMFIGNLLQAAYNTVDSIWVG
                     RFLGPQALASVSVGFPIIFGLIALVTGLTMATTTLVSQYYGARRPDMVSAAISNGLTL
                     LTIMGVIMAAIGYLLRWPLLRLLRAPDDIIGAAASYLGVYTLGFAPSFIYNVASSILR
                     GLGDSRSPLRFLTYATVMNIVLDPIMIFGFGPIPRMEVAGAAWATVISQVFAAALALA
                     YMIGRLRIGTRLRDLARLDWNLTRLTVKIGLPAGAQQTLVSLGMMVIMSTVNQFGSVV
                     VAGSGVGSRLEQFAHLPAFSVGLAVTALVGQNLGAGQKERVSEVVKWGMLLTCTITGA
                     ITLVVQAFPTAVLSLFTKDPQVLAAGTVYLRWISLGFVPFAAMFALTGVLRGAGDTLP
                     TFFITLGSLWLVRVPLVRVLARTSLGVEGAWMAMASGPFVGLLLSYAYYRSGRWKQKA
                     IVSRRPAAGSADLVTTDDIQ"
     gene            complement(4631..>5375)
                     /locus_tag="PLM74_08015"
     CDS             complement(4631..>5375)
                     /locus_tag="PLM74_08015"
                     /inference="COORDINATES: protein motif:HMM:NF014010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HON43056.1"
                     /translation="LVPGEDIVEEAGPKYLDRRMLGTPGIALDLANQETVRMASIVQG
                     MLSRSRKALTEGFTPQIGAELASREELIDSLNRQVVLYLSDMVAKTSVNETQSVRLAG
                     LMHAVGDVERIGDVAEQLMNYAHEKSDTRVVFSDTAVQELHEVFDLADSILQYGIEAL
                     RDGSTKAAEEVYSIHRLLDEATDTLRANHISRLNEGTCVPPAGVIFVEAMNSLERVGD
                     LAVNLADAVMGKKSPRVEEKTRDRREAGV"
BASE COUNT         1131 a         1697 c         1448 g         1099 t
ORIGIN      
        1 cgaggctact actcctttcg cgatctcgcg ctcacatcct gcagcgcctg tggcgaagcc
       61 gggtcggctc ccgtcgttat cgcccttgat ggcattcctg aaggaatcgt tgaacactcc
      121 gattcctgtc gaacgctgct tgcctgcagt gaacatccta gtatggcata gccccgacgg
      181 tccacccgcc cacggctcac cgtacagcag aagcgacggc tcgaccgctc ggagttcgca
      241 tccgatttct ctcatcgtgt cgcagtctat caaggccatc aggtcaaatc tgaatccgtc
      301 cacatggtac tcctggaccc agtatttgag cgaatcgaca atcaacttgc ggaccatcgg
      361 tcgctcagta gcgagctcgt tgccgcagcc cgatccgttg gtgaacatgc cggcagcgtc
      421 cgtgcggtgg tagtagcccg gcacaatcat ctgaaggggc gattcctctg tgctgtaggt
      481 atggttgtac acgacgtcca gaaccactcg aagacccgcc ctatgcaatg cgtgtatcat
      541 ctgcttcact tcggtgatcc gcgtcgccca agactcagga tcgcaggagt acgacccctc
      601 cggaacgaag aaatgaaacg ggtcgtaccc ccaattgtac ccgtcgggcg acagttcatc
      661 gactgaggcg aaatcctgta tgggaagcag gtgcacgtga gtgattccca gttcaactag
      721 gtgatctact cctgtgcgca cacgatacgg gccgcgggtc ccgcgttcgc tcatgcctag
      781 gtacttgccg cggtactgag tgcctgaatc tggcgatatg gagaaatccc tcacgtgcgc
      841 ttcgtacacg acggcatccg ttgccgcagc cagaggagga cgctcatcgc ctgcccaccc
      901 ttccgggtca gtcgatgaca ggtcggctac aacgccgcga cctccattca ccgtaagtgc
      961 gcgcgcgtaa gggtccaccg cctccttcac gcacccgcca tggaataccc tgtagatgta
     1021 gcataggccc gcaagatccc cgggaacgac ggcaagccac gtaccgtcgc agtccgggct
     1081 catcgagact tcccatcccg ccgcggactg cgctgacgag tacagcatca ccgacacgcc
     1141 cagggcagta ggcgcaaaaa cacggaacgc cgtagaatcc ggcgaataga ctgcgccgag
     1201 atcgtcgccg ggatagtaca gctcgaacag ttcctgaccg cccactgcaa cgaccgggca
     1261 cacttcaagg cccatctgtc gcaccaacct tccaccaaac cgcattgtag ctgcacatta
     1321 tcatgataca gtatccggcg gcctggtgtc caacacgcgg tcgtcttcga acccctactt
     1381 accctcgaag aacgtttcca tttcgtccag cgtcttctcg aacaccccca gcgcggcaag
     1441 caccggctca ggtgatgtca tgtccacccc tgcacgcttc agcaggtcaa tggggtacct
     1501 ggcgttaccg ccgctcagga actcaagata gcggtcctgt gcgggctgtc cctggtcgag
     1561 tatactgtct gcaagtgcaa ccgcagccga gaagcctgtg gagtactgat acacgtagta
     1621 ggccctgtag aagtggggga tcctggccca ctcgtacgcg atcgcgtcat ccaacaccat
     1681 atcgggacca aagtagtcgc ggttcagacg taggtagacc tggttcagcg catcgggcgt
     1741 cagggcttgc cctgattcgt gcatctcatg ggtgatcttc tcgaactcgg cgaacatggt
     1801 ctggcggaac actgttccac gaaactcctc cagaaagtgg ttgagcaggt acatcctgcg
     1861 cgcaggatca tccgttctgt ccagcaagta tcgggtgagc agcacttcgt ttacggtcga
     1921 ggccacctcg gccacgaata tgaggtagtc cgagtacacg tatggctgtt ttgcccaggt
     1981 gtagtaggag tgcatggagt gccccatctc gtgcgcgagg gtgaacgcat tgctcaggtt
     2041 gtcctgccag ttcagcaaga cataaggatg gacgccgtac acgccccacg aataggcgcc
     2101 gcttgtcttg ccccggttct ccatcacgtc gatccagcct gactccatcc cagccgaaag
     2161 agcgcgtatg tactccccgc caaggggcgc caggccggtg cgtaccatgg ctttggcttc
     2221 atcgtaagtg acaggccatt ctacatccgg aaccaccggc gcatacacgt cgtacatgtg
     2281 gatctcgggt agatcaagcg ccttcttcct aagacgcacg tatctatgca tcagatcgac
     2341 ccggctcctc acagccgaca ccagattatc gtacgcctct accggtatgt tgttctcatc
     2401 cagtgctgcc tcgcgagcgc tgctgtatcc ccgcacgcgc gcattgaagc tatctgcctt
     2461 gaccgatgca gagtaagttg cagctacggt gtttatcaag gcacgatatg acgagtacaa
     2521 ggcgtcaaac gcatcctttc gcactcgccg atcacggctc tgcatgaacc ggatgtacct
     2581 accgtgggta acctccactg tgttgccgtc cgcgtccacg attgatggga acttgaggtc
     2641 tgcgttgttg agcatggtaa aaatggcagg cactgcatgc ccgatctcac ccgccatggc
     2701 catgatgcgc tcttcagatg cagaaagggt gtggtcgcgc atgcgtctga tgttgtccag
     2761 gaacatcctg tacagctcca gcccaggttc ctccgacatg aaccgctcca gttcgtcgtc
     2821 ggaaatagcc aaaatctcgg gcctgaggaa tgacgtcgcc gacgcggcct ccactgccag
     2881 gctagctgcc ctatcgtgca tgctctggta atcagcattt gcgttgtctt catccttgcg
     2941 catgcggctg taggtgtagg ccttcgtcac aaggtagtcc acttcatccc tgagtttcag
     3001 gcattccagc agcgccttgg ggcctgcgcc cagcctgcct gcgtaggacg ccatgtcggg
     3061 gatcttgttc cgcacggctg acatgtcagc ctcgcacgct tgatccgacg cgtagatgtc
     3121 ttccaccttc cacctgagct cttcagggat atcctttctt tccggaatcg cttcaaccgt
     3181 ctcagccaac tacattcggc ctccttctgg tttctactga atatcgtctg tagtcaccaa
     3241 atccgccgag ccggcggcgg gtcgccgtga cacaatggcc ttctgcttcc atcgaccgct
     3301 ccgatagtaa gcgtagctca gaagcagacc cacgaatggg ccgctggcca tagccatcca
     3361 tgcaccttct acccccaacg aggtgcgagc cagcacccga acgaggggca cccgaacaag
     3421 ccacagagaa ccaagagtga tgaagaatgt gggcaacgtg tcgcctgccc ctctcagcac
     3481 acccgtaagt gcgaacatcg cggcgaatgg gacaaagccg aggctgatcc agcgcaggta
     3541 gacagtgccc gccgcaagta cctggggatc cttcgtgaac aaggacagca cagccgttgg
     3601 gaatgcctga acaaccaggg tgattgctcc ggttatcgta caggtcagca gcatccccca
     3661 cttgaccacc tcagacacgc gctccttctg tccagccccc aggttctgcc caaccaaagc
     3721 ggtgaccgcg agtccaacgc tgaaggcggg caaatgcgcg aactgctcaa gccgcgatcc
     3781 tacgcctgaa cctgccacca ccacacttcc aaactgattc acggtggaca tgataaccat
     3841 catgcccaaa gagaccagag tctgctgagc cccggcaggt agaccgatct tcaccgtgag
     3901 cctggtgaga ttccagtcca gtcgcgccag gtcccggagg cgagtgccga ttctgagtcg
     3961 cccgatcatg tacgcaagcg ccagggcagc cgcaaaaacc tgggagatca cagtggccca
     4021 agcggcgccg gccacttcca tcctgggaat gggcccgaaa ccaaagatca taatgggatc
     4081 caggacgatg ttcatgactg tcgcataggt aagaaatcgc agcggcgacc gcgagtcgcc
     4141 aagaccgcgg aggatagagc tagccacgtt gtagatgaag ctcggcgcga atcctagtgt
     4201 atacacgcca aggtagctcg cggcggcccc tattatgtca tcaggcgccc gcaataagcg
     4261 caggagcggc caacgaagca ggtagccgat ggcggccatt atcacgccca taatggtcag
     4321 aagagttagg ccgttggata tggccgccga caccatatcg ggccgcctgg cgccatagta
     4381 ctgcgatacc agagtggtag ttgccatggt cagccccgta accagggcga tgaggccaaa
     4441 tatgatcgga aagcccacgg agacggatgc caacgcctgt gggcctagga atctgcccac
     4501 ccatatgctg tcgacagtgt tgtatgcagc ttgcagcagg ttcccgatga acatcggagt
     4561 ggcgaaagcg atgaggtgcc tcggtatgct tcctgttgtg aaatcagtag tgcgtacatg
     4621 ctccataatc ctatacgcca gcctcccgtc tgtctcgcgt cttctcttcc actctggggc
     4681 ttttcttccc cattactgcg tcagccaggt tcacggccag atcgcccacg cgctccaggc
     4741 tgttcatggc ctcaacaaat atcaccccgg ccggaggcac acacgtcccc tcgttcagcc
     4801 gtgatatgtg attggcccgg agcgtgtcag tggcttcatc gaggagcctg tgaatagagt
     4861 agacttcctc agccgccttt gtgctcccgt cgcgcaacgc ttcgattccg tactgcagga
     4921 tcgagtcggc taggtcgaac acctcgtgca gttcctgcac ggcggtgtcc gagaagacca
     4981 ctcttgtatc ggatttctcg tgcgcatagt tcatcagctg ctccgccaca tctccgatcc
     5041 tctccacatc gcccacggcg tgcatcagcc ccgccagcct caccgactgc gtttcattca
     5101 ccgacgtctt agccaccata tccgacaggt acagaacgac ctgacggttg aggctgtcaa
     5161 tcaactcttc tctggaggca agttccgcgc cgatctgcgg cgtgaaccct tcggtcagcg
     5221 ccttacgtga tcgagacagc atgccctgga caatgctggc catgcgtacc gtctcctgat
     5281 tggcaaggtc cagtgcgatg ccgggagtcc cgagcatgcg gcggtcgaga tacttcggcc
     5341 ccgcctcctc tacaatgtcc tcgccgggaa ccagc
//
LOCUS       DAOTAC010000174         5374 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_12794_length_5374_cov_4.954127, whole genome shotgun sequence.
ACCESSION   DAOTAC010000174 DAOTAC010000000
VERSION     DAOTAC010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5374
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12794_length_5374_cov_4.954127"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..816
                     /locus_tag="PLM74_08020"
     CDS             88..816
                     /locus_tag="PLM74_08020"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HON43057.1"
                     /translation="MTDDQCSGRDGRNDYELMAEFFDRRVDGYEEHMREIFPAFDEFY
                     SSVAGQIPATGEPLSILDLGCGTGLELDFIFARAPNAVITAVDLSAEMLAKLKAKLER
                     VRYGPQVEIVQGSYLTMPFPAGRFDCVVSVQTMHHFTPHVKRRLYSRIADALKAGGRY
                     IEGDYVVSPGEERSLLARYAELVKSLRAAGAVGEDELVDGKHHIDIPLSVQSQVAVLR
                     EAGFADVEVVFEQGATAVFCAGSR"
     gene            complement(890..3184)
                     /locus_tag="PLM74_08025"
     CDS             complement(890..3184)
                     /locus_tag="PLM74_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HON43058.1"
                     /translation="MRSARIRATLALLLLILYALAFGKPAAAYAGANAEPSGRLELTV
                     LSGSKDVCAECTAMATNMSTGHTAMLTMSPSGTLTAELPAGTYMVIVMPPGGIGSPSA
                     TPTGQIGAVLPAMLNVVIVAGQTARETVVMGGPIMPDEFDASQFEELFDLDSEGGHPQ
                     AADVDLDAVIADDELVALARRYHDLYWGRLTFSERSLVESLTWRMDIRSGGAAPSGDV
                     EEITTAAAAIAAPGASRSASVVLAARAVMEMPDLGLALNNFGAMLRLLGEISDSVVVL
                     RAARAVDPESPMILTNLANSVRELGDLRLAESLYLEALRAGDEFGPALSALGDMYMAR
                     GDYEKAVDMLMRGSKMGFCTAVNDSLVDALDQAYGDSDQVPPRPPDWDSNDSPASAIG
                     VVPLVAQHGHPVLVLPRFGNWGSVDSLAGSMEPLAEIMSRIAVERSSAVQQDVNLKRQ
                     RMATANPRDYEIPGTFPLRFEKQLLHLRLIKDYFWKTAVQAIDRATEQADPDASRERY
                     VSMMQRYALRMAAAGSEQEAREIAAEFCAEAAAFANREFAKNKPIWAAMNTAVVAAIE
                     DYWAFCQPVLDSIYDPITYKLAELDRRITAYSMIQMAYDQTLALAAMPISHFECGNCG
                     GTGKATAPVGDADVPPDPDDKCPFKGGSKFSLSLGPIDYSVTCTTVEIGFAAGTAASL
                     TWDFKNKRVTQIFVGVGVQSGVGPARLGRRFGAQVTFDPDGSVSDISGAASGSVSLGP
                     VSPGVSTNGGLVVGLPFVSIPVGR"
     gene            3582..4676
                     /locus_tag="PLM74_08030"
     CDS             3582..4676
                     /locus_tag="PLM74_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43059.1"
                     /translation="MARLCVVSCGQRKIWSSEPGRGPTPAGEAYVSSFSRLCREYALR
                     FFDKWVILSAKYGFLYPHDTIPDDYNVSFNRARPKPISIDDLRSQIASKGLAEIDTIV
                     VLGGRRYAEVVVAAFGGDYSYELPLAGAAGIGVMMQRLRRALDDDREIRAEAGGMIMD
                     LVHWLQGVEGHKFVPLFELDDNLAVKIKYVGSQNRPVLRRHPQMEQTIIDLVEKGLED
                     PDWEGLIYVMGWHDLPHFVPLFVGKTERRGIRRQVSENIRSIRTNTSAFARWGDNIDY
                     HIGDLSHALFRFKAYREPRKSYERWAATLFEAPGSTHLVQPVFLYVAPWYTYSTGPSG
                     SVCSVKQVTQELIDLASTQFAETLLNARRR"
BASE COUNT         1119 a         1554 c         1563 g         1138 t
ORIGIN      
        1 gcctggctgt agacgagata ttgagggagg caagctcgca tgaagcttga tgctgatgct
       61 gtgttgaact tggcgggacg gtgaaccatg actgacgatc aatgcagcgg gcgcgatgga
      121 cgcaacgact atgaactgat ggcggaattc ttcgacagaa gggtcgacgg gtatgaagag
      181 catatgcgcg agatattccc ggcgttcgat gagttctact cgtctgtagc tgggcagatt
      241 cccgcaaccg gcgagccttt atccattctg gacctgggtt gcggcacagg cctcgagctg
      301 gacttcatct tcgccagggc gcccaatgcc gtaatcactg cagttgacct ctcggcagag
      361 atgcttgcca agctgaaagc caagctcgaa cgcgtccggt atgggcccca ggtggagatt
      421 gtacaaggat cctatctgac catgccgttt cctgcgggcc gttttgactg tgttgtatct
      481 gtgcagacaa tgcaccattt cacgccccat gtgaagcgac gcctgtattc ccggattgcg
      541 gacgcactca aggccggcgg aaggtacata gagggagact atgtggtatc gcctggcgag
      601 gagcgttccc tgctcgctcg atatgccgag ctggtaaagt cgcttcgggc tgccggggcc
      661 gtgggagagg atgagcttgt ggacggtaag caccacatag acatcccgct gagcgtccag
      721 tcgcaggtag cagtcttgag agaagccgga tttgcagatg ttgaagtggt gtttgagcag
      781 ggcgcaactg ccgtcttttg cgcgggcagc aggtgatgtc cgcgtgagtg cgaaatgcct
      841 ccccatcgta gggccggaac tcgattcgac cggcgactga tcacgctctc tagcgtccca
      901 cgggaatact cacgaagggc agacccacta caagccctcc gttcgtgctt actccaggcg
      961 acactggacc aagactaacc gaacctgatg cagcgccgct tatgtccgac accgaaccgt
     1021 cgggatcgaa cgtaacctgt gcaccgaatc tcctaccaag tctggcggga cccacgccgc
     1081 tctggacgcc cacgcccacg aagatctggg tgacgcgctt gttcttgaag tcccatgtca
     1141 gagaagccgc ggtgcctgct gcgaacccga tctccacagt ggtgcatgtc acgctatagt
     1201 ctatcgggcc caggctgagg ctgaatttgc tgccgccctt gaatgggcac ttgtcgtctg
     1261 gatcgggagg aacgtccgcg tcgccgaccg gcgctgttgc cttgcctgtg cctccacagt
     1321 tcccgcactc aaagtgggat atgggcattg ccgccaacgc cagggtttgg tcgtacgcca
     1381 tttggatcat ggagtatgct gtgattcggc gatctagttc agccagcttg tacgtaatcg
     1441 gatcgtatat ggaatccagt accggctgac agaaggccca gtaatcctcg attgccgcta
     1501 ccactgcagt attcatagca gcccagatgg gcttattctt ggcaaactca cggttagcga
     1561 aggccgcggc ctccgcgcag aactcggccg caatctctct tgcttcctgt tctgaacccg
     1621 cggccgccat ccgtagtgcg tatcgctgca tcatggagac gtatctttca cggctggcat
     1681 cggggtcggc ctgctcagtg gcccgatcta tggcctgaac cgccgtcttc cagaaatagt
     1741 ccttgatcag cctcaggtgc aagagctgct tttcaaatct gagcggaaat gtgcccggaa
     1801 tctcgtaatc ccgcgggttg gctgtcgcca tcctctggcg cttcaggttg acatcttgct
     1861 gtactgcgga ggagcgttcg actgcaatcc tgctcatgat ctccgctaat ggttccattg
     1921 aaccggccag cgaatcaaca cttccccagt tgccaaaacg cggcaggacc agaacagggt
     1981 ggccgtgctg ggctaccagg ggcaccaccc caatcgccga tgccggcgag tcgttgctat
     2041 cccagtcggg ggggcgaggc ggcacttggt cagaatcacc gtaggcctga tcaagtgcat
     2101 ctacgagaga atcgttgact gcagtgcaga atcccatctt cgacccgcgc atcaacatgt
     2161 caaccgcctt ttcgtagtca ccgcgcgcca tatacatatc acccagggcc gatagggcgg
     2221 ggccaaactc gtctccggcc cgaagcgcct caaggtatag gctttcagcc aggcgcaagt
     2281 cgccaagctc ccggacggag ttggccaggt tcgtcagtat catgggagat tcgggatcaa
     2341 ctgctctggc tgccctgagc acgacaactg agtcggagat ctctcccagc agacggagca
     2401 tggcaccgaa gttgttcaat gcaaggccaa ggtcgggcat ctccatcacg gctcgggcag
     2461 ccagaactac tgaagccgac cgtgacgccc cgggcgccgc aatcgcagcc gcagcggttg
     2521 tgatttcttc cacatccccg ctcggggcag ctccgcccga acgaatgtcc attctccagg
     2581 taagcgactc gacgagcgac cgttcactga aagtgaggcg gccccaatag agatcatgat
     2641 acctcctagc cagcgccacg agctcatcat cggcgatcac ggcatcaaga tctacgtccg
     2701 cagcctgagg gtgtccgcct tctgagtcga ggtcgaacaa ctcctcaaac tggctcgcat
     2761 cgaactcatc gggcataatg gggcccccca tgaccacagt ctcccgtgca gtctgcccgg
     2821 ccactatgac cacgtttagc atcgcgggca acaccgcccc gatttggcct gttggcgtgg
     2881 cggacggcga cccaatgccg cccggcggca tcacgatcac catgtatgtg ccagccggca
     2941 gttcagctgt gagcgtcccg gacggcgaca tcgtcaacat ggcagtatgc ccggtagaca
     3001 tgttcgtggc catggcagtg cattcggcac acacgtcttt tgagccggaa agcacggtca
     3061 attccaggcg accggatggc tcggcgtttg ctccggcata tgcggccgca ggttttccaa
     3121 acgcgagcgc atacaggata agcaggagta gcgcgagggt agcccgtatc cttgccgatc
     3181 tcatgcgggt ctcctccgat cgtctcatgt tccgtcgcca tgaatcttcg cagctctaaa
     3241 tgactgtcga agcctgatgc gcacacaaag cggtagttgg tttttcaaca attgacaaga
     3301 cggtgcaaga tttctacggg cgagcgacaa tacctttctt gcgaaaacga agactgacgg
     3361 acacgatcgc cggagtcagg agaatcgggc cactctgagt ctgcgcatgc caacaccgtc
     3421 ttccacgtca tgggtctgca aatccctgat cacccggggc gcttcgggta caaccaggcg
     3481 atgcagcata gctcgcagga gaagccttgg ctagagggaa aaaccgctcg atatgagaat
     3541 acctatttgc agcatattct gtgatctatc gggtgagtgt gatggctagg ctctgtgtag
     3601 tttcttgcgg tcagaggaag atctggtctt cggaacctgg tcggggtcct acaccggccg
     3661 gggaggcata tgtcagttcg ttttcgcgtc tgtgcaggga gtacgctctg cggttcttcg
     3721 acaaatgggt cattttgtcg gcgaagtacg ggttcctata tccacacgac actatcccgg
     3781 atgactacaa cgtgtcgttc aaccgggcga gaccgaaacc tattagcatc gatgacctgc
     3841 gaagccagat agcgagtaaa ggccttgccg agatcgacac tatcgtagtt ctcggcggac
     3901 gccggtatgc cgaggttgtg gtggcggcat tcggcgggga ttattcgtac gagctacctc
     3961 ttgcaggcgc cgccggcatt ggcgtcatga tgcagagact caggcgagcg ctagatgatg
     4021 atcgggagat tcgtgcagaa gctgggggaa tgatcatgga tctagttcat tggcttcagg
     4081 gagtagaggg acacaagttc gttccgctgt tcgagctgga cgacaatctc gcggtgaaga
     4141 tcaagtacgt gggaagccaa aatagacctg ttctcaggcg tcatccccag atggagcaga
     4201 caatcatcga tctggtggag aaaggccttg aggatcctga ctgggagggc ctgatctacg
     4261 tgatgggctg gcatgaccta ccccacttcg ttccgctttt cgtaggcaag actgagcgtc
     4321 gaggcatccg ccgccaggtc agcgagaaca tccggagtat tcgcacgaac acgagcgctt
     4381 tcgcaagatg gggcgacaac atcgactacc acatcggaga tctcagccat gccttgttcc
     4441 gtttcaaggc atacagagag cccaggaagt cctatgagcg atgggccgca acgctatttg
     4501 aggcgccagg gtccactcat ctcgtgcagc cggtcttctt gtatgtcgca ccttggtaca
     4561 cctacagcac gggcccgtcc ggcagtgtgt gctcggtgaa acaggtcaca caagaactca
     4621 ttgacctggc ttccacacag ttcgcggaga ctcttctgaa cgcccgcaga cgatagccat
     4681 atcacaagtg gaaaaccgcc cgcgttgctt tgcgtacgag aacgcgggtg ggtgcagttc
     4741 gggtcggggc atgcccgatg ccggcgacga ggtgttgcgg gcgaactggc gtttcctcct
     4801 ttccgtccac acgcttggcc accgccaggc gcctcgcccg gtttgctctc ctttggccca
     4861 tggcctctgg catacatacg gctccgcgac tgtggcgtcc accgagaaaa agcgccgaat
     4921 aggaagtgga cgctgtgcac tacacgtatc catgggtatt cctgggcgtg acgttctccc
     4981 gtggtgggct ctggtggggt tctggggcat cctgtccatt tttgaacagg cttctcgagc
     5041 atcctgttca gatcctgccc ggtttggcag gcgataattg tcagaattcg cgccggcaac
     5101 ggcgcctcct ttttctgtaa tcacgccgca gtcgaatccg caggtagagg tcgtttacat
     5161 aatatggtca tacgcacacg ccttccgagt ctccccggaa aggccccgga gacgggtttc
     5221 agacccctaa taagtcccaa atccgctggg ctccggctcc tgcctgtcaa aaccgggcac
     5281 attctgaaca gcccgcgcag aaaaccgggc acatttcttc tatacgttac ccatatccat
     5341 ggatcggccc gggtacaaag gccgaagcgc cccg
//
LOCUS       DAOTAC010000175         5356 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_12852_length_5356_cov_4.481041, whole genome shotgun sequence.
ACCESSION   DAOTAC010000175 DAOTAC010000000
VERSION     DAOTAC010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5356
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12852_length_5356_cov_4.481041"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="PLM74_08035"
     CDS             complement(<1..572)
                     /locus_tag="PLM74_08035"
                     /inference="COORDINATES: protein motif:HMM:NF024912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HON43060.1"
                     /translation="MRHGGITPARMGAAGGANSADRPREECGVFGACMNPGADAALTT
                     FYGLMSLQHRGQDSAGIASSHGERVRVVKGPGLAAEVFHEDALARLSGYVAIGHVRSA
                     ARADEDDTAIQPFVFRYTGGMMAVAHNGSITNGKALRERLGRSGVVFQSESDAEVIGS
                     LVARYYSLGMIGAIERAMEDLEGAYSLLVMA"
     gene            complement(554..2776)
                     /gene="purL"
                     /locus_tag="PLM74_08040"
     CDS             complement(554..2776)
                     /gene="purL"
                     /locus_tag="PLM74_08040"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HON43061.1"
                     /translation="MGLADEEYERIRQLLGRDPNYVELGMFSVMWSEHCSYKNTRAVL
                     RGLPTSGPQVLQGPGENAGIVDVGDGLAVVFKIESHNHPSAVEPYQGAATGVGGIIRD
                     IFTMGARPIASLDSLWLGDLSSPRARRLFSGIVAGISGYGNAVGIPTVGGQTVFHSSY
                     ESNPLVNAMCVGVMRHEDIKRGAATGVGNSVMVIGSTTGRDGIHGATFASADLTETSI
                     ERKSAVQVGDPFREKLLLEACLELVARGCVLGIQDMGAAGLTCSSCEMASRGGTGMII
                     DLDRVPRRETGMTPYEVMLSESQERMLLVPTPGREDEVREVLSKWGLEAVVVGEVTGD
                     GMVTLIEGGDQVAHVPAKALADDAPVYHRESSKPEYVDRLAAFDTIGVPACTDLAGAL
                     LQLVASPSIASKRWVWEQYDHQVGAGTVIGPGSDAAVVRLPGSKRGVALTTDCNSRYC
                     YLNPRLGAAHAVAEAARNVACTGARPLAVTNCQNFGNPYKPEVFWQFREAIAGMSEAC
                     RALGTPVTGGNVSLSNEDGETAIRPTPVIGMVGAIDDVELRVPMGFQAEGDIVVLLGY
                     TRDEIGASEYLAVVHGVEAGQVPALDLEAEGRLCRLLATAASRKLLSSAHDTSEGGLA
                     VALAECAIAGRLGATLRIDCAGHDAGLAPDRFLFSESGARALVTVEQSRLDEFLALAA
                     SLNVPAAAVGCVGGDRLCVEFGETPKTCTGAGAERRLDVSVDALSKAYEEAIPCAMEG
                     "
     gene            complement(2814..3524)
                     /gene="purQ"
                     /locus_tag="PLM74_08045"
     CDS             complement(2814..3524)
                     /gene="purQ"
                     /locus_tag="PLM74_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HON43062.1"
                     /translation="MKFGIVVFPGSNCDRDVQTAARAAGYDAEFVWHTNTSVDGFDCL
                     VLPGGFSYGDYLRTAAIARFSSVMEPVAQYAASGRPVIGICNGFQVLLEAGLLPGAML
                     RNTSCKFICRPQRVRVEVESVRTPFTLKLRPGQVITLPIAHGEGNYHVDGETLAQMER
                     GSQVVFRYCDESDEPTPGSNPNGSLGNIAGICNKAGNVLGMMPHPERAAESILGGADG
                     RLLFDSVAQWVQGGGRIV"
     gene            complement(3521..3781)
                     /gene="purS"
                     /locus_tag="PLM74_08050"
     CDS             complement(3521..3781)
                     /gene="purS"
                     /locus_tag="PLM74_08050"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007473712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HON43063.1"
                     /translation="MVYVADIVVTLKKSILDPQGDAVRSALRSMGYTGVQDVRIGKRI
                     EVRLEAASEAECRQAVDEMCSRLLANTVVEAYDFSIKEAPRG"
     gene            complement(3868..4395)
                     /gene="purE"
                     /locus_tag="PLM74_08055"
     CDS             complement(3868..4395)
                     /gene="purE"
                     /locus_tag="PLM74_08055"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HON43064.1"
                     /translation="MSEVAARPGQSGSGVRVGVVMGSQSDLAKMSKARETLSELGIEC
                     ELRVISAHRTPEEAAAYARNAEERGLCVIIAGAGGAAHLAGIMAAYTALPVIGVPIAS
                     GALGGFDALCATVMMPSGVPVATVAIDGAANAGILAAQIIALSDGEVKQRLVEYRWTI
                     AEKVRQADAQVRALQ"
     gene            complement(4674..>5356)
                     /locus_tag="PLM74_08060"
     CDS             complement(4674..>5356)
                     /locus_tag="PLM74_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43065.1"
                     /translation="EGVIGLIWLMFFVGSIVRAIAQGKRRAEQEAARRAMRRAQGLPR
                     QAPTQHAPRGQVFRPGWPVYPGQPAQPVVVVEQRESQSQGEGEGQSGGEGESAEGEPA
                     EGDSRDIRRRLRQERSEEWQSDRARTWEMPVDTGSLGAEGEAVDEPIAKSAEAFEADL
                     DREALDWVLDEDMVAQAGTDVGAAERLERDVGMWRIPGRVEPEHALAGIVWGTVLAAP
                     RCRLTRHR"
BASE COUNT          989 a         1914 c         1447 g         1006 t
ORIGIN      
        1 gccatcacca gcaaggagta ggcaccctcc aggtcctcca tagcgcgttc tatcgcgccg
       61 atcatgccaa gtgagtagta gcgggcaacg agcgagccaa tgacctcggc atccgactcg
      121 ctctgaaaca caactcccga gcgccccaat cgctcgcgca gggctttgcc attggttatg
      181 cttccattgt gggcaacagc catcatcccg cctgtataac gaaagacgaa gggttgtatg
      241 gccgtgtcgt cttcgtctgc gcgcgcggca ctgcgcacgt ggccgatagc cacatatccg
      301 gacagacggg caagggcatc ttcatgaaag acctcggcag caagcccggg acccttcacg
      361 acccggacgc gttccccgtg ggatgacgcg attcctgcgc tatcctggcc cctgtgctga
      421 agggacatca agccgtagaa ggtggtcagc gcggcgtcgg cgccaggatt catgcacgcc
      481 ccgaagacac cgcactcctc cctgggccgg tcggcgctat tcgcgccgcc cgctgctccc
      541 atgcgcgcag gcgttatccc tccatggcgc atggtatcgc ctcctcgtac gccttcgaca
      601 gcgcgtcgac actcacgtca aggcgccgct cagctccggc gccagtacac gttttcggtg
      661 tctccccaaa ttccacacac agcctatcgc cgccgacaca gcccacggcc gctgcgggca
      721 cgttcaggct ggctgcaagc gctaggaact catccagccg gctctgctcg acggtgacca
      781 atgctctcgc cccgctctcg ctgaacagga aacggtcggg ggcaagcccg gcatcatggc
      841 cggcacagtc aatcctcagg gtcgcaccca aacgcccggc gatggcgcat tcggccagcg
      901 ccactgccag accgccctcg gatgtgtcat gcgccgagct caggagcttc ctcgacgccg
      961 cagtcgcaag cagccggcat aggcggcctt ctgcctcgag gtcgagcgcc ggcacctgcc
     1021 cggcctctac cccgtgtacg accgccaggt actccgatgc cccgatctca tcgcgcgtgt
     1081 agcccagcag cacaacgatg tcgccctctg cctggaatcc catgggcaca cgcagctcca
     1141 cgtcgtctat tgcaccgacc atccctatga caggcgtggg acgtatggcc gtctcgccat
     1201 cctcgttcga cagactgacg ttgccgcctg taacaggcgt tccaagcgcg cggcacgcct
     1261 ccgacatccc ggcgatagcc tcgcggaatt gccagaacac ctcgggcttg tacgggttgc
     1321 cgaagttctg acagttggtc acggccaatg ggcgcgctcc ggtgcaggcc acgttgcgcg
     1381 cggcctcagc cacggcatgt gccgcaccca ggcgcgggtt gagatagcaa tacctagagt
     1441 tgcagtcggt cgtcagtgcg accccccgct tggatccggg cagacgtact accgccgcgt
     1501 ccgagccggg ccctatcacc gtgcctgccc cgacttggtg atcgtactgt tcccaaaccc
     1561 acctcttgct ggcgatgcta gggcttgcta ccagctgaag cagggcgcct gccaagtccg
     1621 tgcacgccgg aacgccgatc gtgtcgaacg cagccagcct gtccacatat tctggtttcg
     1681 atgattccct gtgataaacg ggcgcgtcat ctgccaaagc tttcgcaggc acgtgcgcaa
     1741 cttgatcccc gccttcgatt agggtaacca tcccgtctcc cgtgacctcg cccactacca
     1801 cggcctccag gccccacttg ctgaggactt cacggacctc atcctcccgg ccgggagtgg
     1861 gcaccagaag catcctctcc tgtgactccg acagcatcac ttcgtatgga gtcatgcccg
     1921 tttcgcgccg cggcactcta tccaggtcaa taatcatccc agttccgccc cgcgacgcca
     1981 tctcacatga cgaacatgtc agcccggcag cgcccatgtc ctggatgccc agcacacatc
     2041 cgcgcgccac cagctccagg cacgcctcga gcagcaactt ctcgcggaag gggtcgccta
     2101 cctgaaccgc cgacttccgc tcaatggacg tctcggtcag gtcggccgaa gcgaaagtgg
     2161 cgccgtgtat cccgtctcgg ccggttgtgg agccaatgac catgacggag ttgccgacgc
     2221 ccgtggcggc gccgcgcttg atgtcctcat ggcgcatgac gcccacgcac atggcattga
     2281 ccaacggatt gctctcgtat gaggagtgaa agaccgtctg gcccccgacg gtagggatac
     2341 ctacggcgtt tccgtaacct gagatgcccg ccactatccc tgaaaacaac ctgcgcgccc
     2401 gaggcgagct gagatcgccc aaccacagag agtcgagcga ggcaatggga cgcgcgccca
     2461 tggtgaagat gtccctgatg atgccgccca cgccggtggc cgcaccctgg tatggctcga
     2521 ccgccgaagg atggttgtgg ctctcgattt tgaagacgac ggccagtccg tcgccgacgt
     2581 cgacgattcc ggcgttctcg cccggcccct gcagaacctg cggtcctgaa gtgggcagcc
     2641 cgcgcaggac ggctcgcgtg ttcttgtagg agcaatgctc ggaccacatg accgagaaca
     2701 tgccgagctc aacgtagttc gggtcccgcc caagcagctg gcggattcgc tcgtattcct
     2761 catccgcgag gcccatcgtc cgccatatgc actcctgttc tctgatcaac tcatcagaca
     2821 atccggccac ctccctgcac ccactgggca actgagtcga ataacagcct tccgtccgca
     2881 ccgccgagta tactttccgc ggcacgttca gggtgaggca tcatgcccag cacattgcct
     2941 gccttgttgc agatgccggc gatgttgccc agactcccgt tggggttgga cccaggcgtc
     3001 ggctcgtcgg actcgtcgca gtacctgaac accacctggc tgccccgttc catctgggcg
     3061 agggtctcgc cgtcgacatg gtaattgccc tcgccgtgcg ctataggcag cgtgattacc
     3121 tgacccgggc ggagctttag cgtaaacggg gtacggaccg attccacttc cacccgaact
     3181 cgctgtggtc tgcatatgaa cttgcagctg gtattgcgca gcatcgcccc gggaagcagc
     3241 cccgcctcga gcaagacctg aaatccgttg cagatcccga tgaccggacg gccggatgcc
     3301 gcgtactgag cgaccggctc catcaccgac gagaaccgcg caatggctgc agtgcgcaag
     3361 tagtcaccgt atgagaatcc ccccggcagc accaggcagt cgaacccgtc tacggatgtg
     3421 ttagtatgcc aaacaaactc ggcatcgtag cccgctgcgc gcgccgcagt ctgaacgtcc
     3481 ctatcgcagt tggacccggg gaacaccact atgccaaact tcatcccctg ggcgcctcct
     3541 tgatgctgaa gtcgtaagct tccacaactg tgttggccag gagtctcgag cacatctcgt
     3601 ccactgcctg cctgcactcg gcttcgctgg ccgcctcaag gcgaacctcg atgcgttttc
     3661 cgattctcac atcctgcaca ccggtgtagc ccatagacct cagggccgac ctgacagcat
     3721 cgccctgcgg gtcaagtatg gatttcttca acgtcacaac gatatcggct acatatacca
     3781 atcaaccgca ccccctgcca tctttgtcgc cgcctgtacc tgcccgtatc tgtagttgcc
     3841 tgtacctttg cctgactgtg cctgtgccta ctgcaatgcg cggacctgtg cgtccgcctg
     3901 acgtaccttc tcagctatcg tccaacggta ttccaccaaa cgctgcttca cttccccgtc
     3961 tgacaaggca atgatctgag ctgcgagtat ccctgcattg gcagccccat cgatcgctac
     4021 cgtagccaca ggcacgccag acggcatcat cactgtcgcg cacagcgcgt cgaaccctcc
     4081 caacgcgcct gatgctatgg gaacgccgat cacaggcaat gcggtgtaag ctgccattat
     4141 gcctgccaga tgagccgcgc cccccgcccc ggcgatgatc acgcacagcc cgcgctcctc
     4201 ggcattgcgg gcataggctg cggcctcttc gggagtccta tgtgcggaga ttactctcag
     4261 ctcacactcg attcccaatt ccgaaagcgt ctcccgggcc ttggacatct tggccagatc
     4321 cgactggctc cccattacca cccctacgcg cactccagag cctgactggc ccggccgggc
     4381 ggctacctcg ctcatacacg tcccctcctc atggtgttac gataaccgga agcaggcaca
     4441 taatcgcaaa tcaaaaaaag aaaagccccg gcggttcact acggtgagtg aaacctgacc
     4501 gccggggctt ttgtccctag aggtgtagcg ccgccatggc gcctgcgccg ctcggaccga
     4561 accttccgcc gcgtacccac gcggcacgga agtggaaccc taggcgcact ttcgctcgtg
     4621 aaagcaatgt attcagttga acttgctagc ttgcctacct caagcttaca ctactacctg
     4681 tgcctcgtca acctgcatcg cggcgctgcg agcacagtcc cccatactat ccctgcaagg
     4741 gcatgctccg gctcaacacg gcctgggatc cgccacattc cgacatctct ttcaaggcgt
     4801 tcggcggccc ccacatcggt gccggcctgg gctaccatat cctcatcaag gacccaatcc
     4861 agcgcctccc gatccagatc agcctcgaac gcttcagcgg acttggctat cggttcatcg
     4921 acggcttccc cctcggcgcc cagagaaccg gtatcgacgg gcatttccca cgtgcgcgcc
     4981 ctgtcggact gccattcctc tgaccgctcc tgcctcagtc gacgccgtat gtcgcggctg
     5041 tcgccttccg caggctcgcc ttccgcagac tcgccctctc ctccgctctg gccctcgccc
     5101 tcgccctggc tctggctctc acgctgttcg accacaacca ccggctgagc cggctgaccg
     5161 gggtaaaccg gccagccggg cctgaacacc tgcccgcgcg gggcatgctg ggtcggcgcc
     5221 tgcctcggaa ggccctgcgc acgccgcatg gcccttctgg cagcctcctg ctcggcacgc
     5281 ctcttgccct gggcaatagc acgcactatc gaccccacga agaacatcaa ccagatcaac
     5341 ccgatgacgc cttcta
//
LOCUS       DAOTAC010000176         5353 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_12863_length_5353_cov_4.914496, whole genome shotgun sequence.
ACCESSION   DAOTAC010000176 DAOTAC010000000
VERSION     DAOTAC010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5353
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12863_length_5353_cov_4.914496"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="PLM74_08065"
     CDS             <1..574
                     /locus_tag="PLM74_08065"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HON43066.1"
                     /translation="RLAGGLPKMLGIVRDDYASLAQAMHDALPRVDMLIVSGGSSIGA
                     RDFAREAIDSLGKPGVLVHGVAVKPGKPTIMAVADGVPVFGLPGHPVSCLTIFRLLVE
                     PAIARMMGGELARRELSARIGANVASAAGREEYVSVRIARRDGETWADPIFSKSALIS
                     GLAQADGVARIPAGIEGVQKGDTVTVLAWR"
     gene            580..2514
                     /locus_tag="PLM74_08070"
     CDS             580..2514
                     /locus_tag="PLM74_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin biosynthesis protein"
                     /protein_id="HON43067.1"
                     /translation="MKRNVYLDEAPLDEAVQEFFTALRGRGVIGALPGEWIAVDVAAL
                     GRITAEPVFAKVSSPHYHACAMDGVAVKSDSTFAASERRPVTLKLGEDAIMADTGDPL
                     PFGFDAVIMVEDLVETTDDSVTIIASAKPWQHVRSVGEDIVATELVLPENHAIRAVDI
                     GAMLAAGVTQVQVRRRPRVAIIPTGDELVAPGSRLQAGDIVEFNSRIIGGFVSEWGGE
                     VVFHDIVRDDFDAITQAVRQAAAESDIVIVNAGSSAGSEDYTSAVVERLGEVIVHGVA
                     IKPGKPTILGLAEGRPFIGVPGYPVSAALACELFLRPLMARMLASPVEPLPQVTATAT
                     RRIVSTPGVTEFVRVKVGDVGGKLVVTPLPRGAGAIMSLVKADGIVRVPPSAQGVDEG
                     AQVQVELLRSLDQIRRTIMAIGSHDLALDILSTFLARDYPGLSLSSAHVGSMGGLLAM
                     RRGEAHIAGTHLIDEETGEYNVPYVKRLLDWENYALVNLVRREQGFIVPPGNPKNIAG
                     FGDLVRRGVVFVNRQRGAGTRVLLDMELGKLGIDPDAVRGYSHEEYTHMGVAASVANG
                     AADVGLGIHGAAVALNLDFVPVSTEQYDLLATREFLESEMMSALLAVIRSDEFKRAVL
                     AMGGYDLSDTGKVVWQGWAS"
     gene            2493..3512
                     /gene="moaA"
                     /locus_tag="PLM74_08075"
     CDS             2493..3512
                     /gene="moaA"
                     /locus_tag="PLM74_08075"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HON43068.1"
                     /translation="MAGLGELVDRFGREITYMRVSVTDKCNLRCVYCMPEEGVKARAH
                     GEMLTMEEFARVVRIASTMGISRVRITGGEPLARKGVVSLVRAVAAIPGIRDISMTTN
                     ATMLAPVADELGRAGLKRVNISLDSLKPDTYARLTRRGDLASAVVGIHAAERAGLRPI
                     RLNAVVLRGINSDEIESLAALTLKHCWDVRFIEFMPFWGNGGLFGEECVVPIDEIRRR
                     VLAMGVEDRSAQRAAAPEENRSGPASYLRLRGGLGRVGFISRDGDEFCAACNRIRLTA
                     DGMFRPCLLSETSIDVKTALRGGADDNVVADLIRRAVAAKPESGRSRGADGGWEARPL
                     SQIGG"
     gene            3569..>4145
                     /gene="moaC"
                     /locus_tag="PLM74_08080"
     CDS             3569..>4145
                     /gene="moaC"
                     /locus_tag="PLM74_08080"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HON43069.1"
                     /translation="MTLDEPSGIPAPGAVPSLTHLDEAGKARMVDISGKPETHREAVA
                     ECRITMNPNTLALIERGEGPKGEVFGVARVAAIMAAKNTSSIIPMCHPIALTGIDVDF
                     VADETAGAVDVRVAVRTLGRTGAEMEALSGAAVAALTVYDMCKAVDRSMTIGDLRLVF
                     KSGGKSGVFFRDDDGPPGDVPNHDDVPNREDV"
     assembly_gap    4146..4245
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4321..5256
                     /locus_tag="PLM74_08085"
     CDS             4321..5256
                     /locus_tag="PLM74_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005331825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HON43070.1"
                     /translation="MGDRAAEGVVVAVCISETKGVPKQDVGSAMLVDNHGLDGDAHAG
                     RWHRQVSLLAEESIAKMRAKGLDVGPGAFAENITTRGLELPILPIGTRLGVGSEALLE
                     VTQIGKECHSKCAIFQQVGECVMPSEGIFAKVLHGGIVKAEDPIRVLPAINVGVLTVS
                     DKGSRGEREDLSGAAIERTVSGMAVVVERAIVPDEFDAIAEELRRMADKLNVNLILTT
                     GGTGFAPRDVTPEATLCLLDKLAPGIPEAMRAASLAKTSHAMLSRAIAGIRGSTLIVN
                     LPGSPRAVQECLEVLMPALPHAITTLAGDGGECAR"
BASE COUNT         1056 a         1464 c         1766 g          967 t
ORIGIN      
        1 ccggttggca ggggggcttc cgaagatgct gggcatcgtc cgcgacgact atgcaagtct
       61 cgctcaggcg atgcatgatg ctctgccgcg agtggacatg ctcatcgtgt cggggggcag
      121 ctccataggg gctcgggact ttgcccgcga ggccatagat agccttggca agccgggtgt
      181 tctggtacat ggtgttgccg tgaagcctgg caaacctacg attatggctg tagcagacgg
      241 cgtgcccgtg ttcgggctac ctggccaccc tgtgtcctgc ctcaccatat tcaggcttct
      301 ggttgagccc gccatcgcga gaatgatggg gggcgaactc gcaagacgcg aactctccgc
      361 gcgaatcgga gccaacgtgg ctagcgccgc aggcagagag gaatacgtgt cggtcaggat
      421 cgcccggcgc gacggcgaaa cctgggccga tcccatattc agtaagtctg cgctcatatc
      481 cggccttgcg caggccgacg gggttgccag aatacctgcg ggaatagaag gggtccagaa
      541 gggtgacacc gtcaccgtgc tggcgtggag gtaagatgcg tgaaacgcaa tgtatatcta
      601 gatgaagcgc cactggatga ggcggtgcag gagttcttca ccgcccttcg gggacgggga
      661 gtcatcggcg cgctcccggg agagtggatc gccgtggacg tcgccgccct gggacgtatc
      721 acggcagagc ccgtattcgc caaggtctcg tcgcctcact accacgcatg cgccatggat
      781 ggagtagcag tcaagtctga ttcgactttc gcagccagcg aacgccgtcc cgtcacactt
      841 aagctgggcg aggatgccat catggcggac accggcgatc ctctgccctt cggattcgac
      901 gcggtcatca tggttgagga tttggtggag acgactgatg atagcgtcac tatcatcgcg
      961 tcggccaaac cctggcagca cgtgcgcagc gtgggagagg acatagtcgc cacggaactg
     1021 gtgctccccg aaaaccacgc tatccgtgcc gtcgatatcg gcgccatgtt ggctgccggt
     1081 gtcacccagg ttcaggtgcg ccgtcgtccg cgtgtggcga tcattcccac cggagacgag
     1141 ctggtcgccc cgggttccag actccaagca ggcgacatcg ttgagttcaa ctccaggatc
     1201 atcggcgggt ttgtgagcga gtggggcggg gaagtcgtgt tccacgacat tgtgcgcgat
     1261 gactttgacg cgatcacgca ggctgtgcga caggctgccg ccgagagcga catcgttatt
     1321 gtgaacgccg gttcgtctgc gggaagcgag gactacacca gcgcggtcgt ggagaggttg
     1381 ggcgaggtca tcgtacacgg tgtggcgatc aagccgggca agccgacgat cctggggcta
     1441 gcggaaggac ggcccttcat cggtgtgccg ggctatcctg tttctgcggc gcttgcgtgc
     1501 gagctgttct tgaggccgct catggccagg atgctggcga gcccggttga accgctgcca
     1561 caggtgaccg ccaccgccac gcgacgcata gtgtctacac ccggcgttac ggaattcgtc
     1621 agagtcaaag tcggcgatgt aggcggtaag ctggtcgtga ctccactgcc gcgcggcgcc
     1681 ggcgccatca tgtcgcttgt gaaggctgat ggaatcgtgc gagtgccccc ttcggcccag
     1741 ggtgtcgacg aaggcgccca ggtgcaggtg gagctgctcc gcagcctgga tcagatcagg
     1801 cgcacaatca tggccatcgg cagccatgat ctagccctcg acattctctc caccttcttg
     1861 gcgcgcgact atcccggcct gagcctgtcg tcggctcatg tgggcagcat gggcgggcta
     1921 ttggcgatgc gcaggggcga ggcccacatt gcgggcacgc accttattga cgaggagaca
     1981 ggcgagtaca acgtcccata cgtgaagcga ctgcttgact gggagaacta tgccttggtc
     2041 aacctggtcc gcagagaaca gggcttcatc gtgcccccgg gcaaccccaa gaacatcgcc
     2101 gggttcggcg accttgttcg ccggggagta gtcttcgtaa acaggcagag gggcgccggc
     2161 accagggtct tgctcgatat ggagcttggc aagctcggca tcgatcccga tgcggtgaga
     2221 ggttattccc acgaggaata cacgcacatg ggggttgcgg cctctgttgc gaacggcgcg
     2281 gccgacgtgg ggctcggcat tcatggcgca gctgttgcac tcaatctcga cttcgtcccg
     2341 gtatcaacag agcaatacga tctgcttgct accagggagt tcctggagtc cgagatgatg
     2401 agcgcgctgc ttgctgtgat cagatccgac gagttcaagc gcgccgtgct agccatgggc
     2461 ggatacgatc tgtcggatac cgggaaggtt gtgtggcagg gttgggcgag ctagtcgaca
     2521 gattcgggcg ggaaatcacc tacatgcgag tctcggtcac tgataagtgt aacctgagat
     2581 gcgtgtactg catgcccgaa gagggggtca aggctagggc acatggtgag atgctcacca
     2641 tggaggagtt cgcacgggtg gtacgtatcg cttcgacgat gggcatatca cgagtgcgca
     2701 tcacaggcgg cgaacccctg gcaaggaagg gcgtcgtttc gctggtgcgg gcagtagctg
     2761 caattcccgg gatacgcgac atatcgatga ctacgaacgc gacgatgctc gcgcccgtgg
     2821 cagacgaact agggagggcg gggttgaagc gcgtcaatat cagcttggat tcgctcaagc
     2881 ctgataccta cgcgcgcctg acgagacggg gcgacctggc ttcagctgtt gtaggcattc
     2941 acgcagcaga gcgtgcaggc ctccgcccca taaggctgaa cgcggtcgtg ctcagaggca
     3001 tcaactccga cgagatcgag agccttgccg ccctgactct caagcactgt tgggacgtaa
     3061 gattcataga attcatgcca ttctggggaa acggcggtct tttcggcgag gagtgcgtag
     3121 tcccgataga cgaaatccgc cgtcgcgtgc tggcgatggg cgtcgaggat agatccgcgc
     3181 agcgggctgc ggcccccgag gagaaccgct ctggtcccgc gagctatctc aggctccgtg
     3241 gcggtctggg ccgggtcggt ttcatctctc gagacggtga cgaattctgc gcagcatgta
     3301 acagaatccg tctcactgct gatggaatgt tcagaccatg cctgctttcg gagacttcga
     3361 tagatgtgaa gacagcccta cgcggcggcg ccgatgacaa tgtcgttgcc gatctcatcc
     3421 gcagggctgt ggcggccaag cccgagagcg gcaggtcgcg gggcgccgac ggaggttggg
     3481 aggcccgccc tctgtcgcag ataggcgggt aggtcgagcc actagtgaag agacaagatt
     3541 ggggagacaa gattggggag acaacaccat gacgctagat gaaccaagtg gcatcccggc
     3601 gccgggcgca gttcccagcc tcacccatct ggacgaggcg ggcaaagcgc gcatggtcga
     3661 catatcaggc aagccagaga ctcatcgaga ggccgtggcc gagtgcagaa tcacgatgaa
     3721 cccaaacacg ctggcgctga tcgagcgggg tgaggggcca aagggagaag tgttcggagt
     3781 agcccgggtg gcggcgatca tggctgcgaa gaacacgtct tcaatcatcc caatgtgtca
     3841 cccaatagcc ctcacgggca tcgatgtgga tttcgtcgca gatgagacgg cgggcgctgt
     3901 ggatgttcgg gtcgcagtgc gaacgttggg ccgaacaggg gctgagatgg aagcattgag
     3961 cggcgcggca gtggccgcac tcaccgtgta cgacatgtgc aaggcggtcg acaggtcgat
     4021 gaccataggc gaccttaggc tggtgttcaa gtcgggcggc aagagcggcg tattcttccg
     4081 cgatgatgac ggcccgccag gcgatgtgcc gaaccatgac gatgtgccga accgtgagga
     4141 tgtgcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngaacc gtgaaggcgg
     4261 gctgaaccgt gaggatgggc tgggctttga ggataggcgg cgccatgagg aggagcgatg
     4321 atgggagata gagccgcgga aggcgtagtt gtagctgtct gcataagcga gacaaaagga
     4381 gtgcctaagc aggacgtggg cagcgcaatg cttgtagata accacggcct tgatggcgat
     4441 gcccacgccg gcaggtggca tagacaggtc agcctgctgg ccgaggagag catagccaag
     4501 atgcgcgcca aaggcctcga cgtgggcccc ggagcatttg cggagaacat cacaacgcgc
     4561 gggctcgagc ttccgatact ccccattgga acccgcctgg gagtgggctc cgaagccttg
     4621 ctggaggtga cccagattgg caaggagtgc cactccaagt gtgccatttt tcagcaagtc
     4681 ggcgagtgtg tgatgccctc tgagggtata tttgcaaagg tgctgcacgg aggcatcgtc
     4741 aaggccgaag accccattcg agtcttgcct gccatcaatg ttggagtgtt gactgtcagc
     4801 gacaagggtt cccgcggaga gcgggaggac ctgagcggcg ctgcgatcga aagaaccgta
     4861 tcgggcatgg ctgtcgtagt tgagcgagcg atagtgccgg acgagttcga cgccatagcc
     4921 gaagagctcc gccgcatggc cgacaaactc aatgtgaacc tgattctgac cacaggtggt
     4981 accggtttcg cccctcgtga cgtgactcct gaggcgaccc tctgcctcct cgataagctg
     5041 gcccccggta ttccggaagc catgagagcg gccagcctgg ccaagacctc ccatgccatg
     5101 ctgagcagag ccattgcagg catacgcggc agcaccctga ttgtgaacct gccaggcagc
     5161 cccagggccg ttcaggagtg cctggaagtg ctaatgcccg cgctccctca cgctatcaca
     5221 acactggccg gcgacggtgg agaatgcgcc agataggccc gccaaccaag ccagatgaac
     5281 gaacgggcgt gctaggcgcg gaaagacgac tgctgtggct cagtgatata cccccggggg
     5341 tatccgccca ggg
//
LOCUS       DAOTAC010000177         5293 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_13024_length_5293_cov_4.229477, whole genome shotgun sequence.
ACCESSION   DAOTAC010000177 DAOTAC010000000
VERSION     DAOTAC010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5293
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13024_length_5293_cov_4.229477"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..645)
                     /locus_tag="PLM74_08090"
     CDS             complement(106..645)
                     /locus_tag="PLM74_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013011604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HON43071.1"
                     /translation="MRREIRLSGYGGQGLILAGIILAEAAMHDGHETVQSQSYGPEAR
                     GGASKAEVIISDEPIDYPKVTAPDVLLLMSDAACKRYACTVEPGGTLIVDSGLVANVP
                     ENRGVVHAIPITDISVEATGRAITANIAALGVLNTVAKLVSPESLEKAIAARVPRGTV
                     EINMKAFRAGVDAAQKAGK"
     gene            complement(642..1466)
                     /locus_tag="PLM74_08095"
     CDS             complement(642..1466)
                     /locus_tag="PLM74_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012447938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HON43072.1"
                     /translation="MAGSIDEYLRTQFMPHIWCPGCGHGIILNALLRAIARLDLPKDQ
                     VCVVSGIGCASRAPGYLDFNTLHTTHGRALAFATGIKLAQPDLHVIVVTGDGDGAAIG
                     GNHLIHAARRNMGLTTIMFNNSIYGMTNGQYSPLTPSGRLATTAPYGNVERNFDMCEL
                     VKASGANYVARATVAHARQLSTYIERAITTPGFAFVEAVGNCPTYFGRRNKLGGPVEM
                     VETLKKNSITIAAADKLDPAEVKGKIIIGEFYRGDAPGYIELYDGVIERAKEAAGK"
     gene            complement(1470..2612)
                     /locus_tag="PLM74_08100"
     CDS             complement(1470..2612)
                     /locus_tag="PLM74_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HON43073.1"
                     /translation="MADSCDTRVKLLQGNESCAEAALLAGVRFFAGYPITPSSEIAEI
                     LSERLPQLGGKFIQMEDEIASMAAVVGASLAGMKAMTATSGPGFSLKQENLGFASFTE
                     VPCVIVNVQRTGPSTGLPTSPGQGDLMQARWGTHGDHPVVALYPSSVSEAFDLTVKAV
                     NIAERLRVPVILLMDEVVAHMRERVVVPCQDEIEIAWRPRPTVPPSDYLPYAAGPDGV
                     PAMADFGTGYRWHVTGLYHDVTGFPTSAPAKVDALVRRLCGKITNHKRDITFTWDEHM
                     DDARVAVVACGIVARSARRAVRLAREKGIAAGLFQPKTIWPFPDEQVAEIAARVDRII
                     VPEMNMGQLVGEVERAARGKCEVIGHSRVDGEPITPEEILARLEEV"
     gene            complement(2602..2853)
                     /locus_tag="PLM74_08105"
     CDS             complement(2602..2853)
                     /locus_tag="PLM74_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010939230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HON43074.1"
                     /translation="MAGDPEAPVLVRREWCKGCGICIAMCPKKVLEAGPDGKALVARP
                     EDCIKCEMCEIMCPDFAVRVVAKKAAAPAQGREAVRDGR"
     gene            complement(3024..3386)
                     /locus_tag="PLM74_08110"
     CDS             complement(3024..3386)
                     /locus_tag="PLM74_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43075.1"
                     /translation="MNVPEVVVRRIYVSGSMSNSRSGLAFRLKNTLVSGHLTAAGAME
                     VDGETVPPSGIIFRIAGAEVKASDVSDQSPLSLPAGMELEVNAVGVVLNPGRHQVRFP
                     VVLKELGPITLTLSDEIR"
     gene            complement(3489..4157)
                     /locus_tag="PLM74_08115"
     CDS             complement(3489..4157)
                     /locus_tag="PLM74_08115"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HON43076.1"
                     /translation="MARLLVVRHGETMFNREKRFQGWLDIPLSDFGSAQATACASYLA
                     TSPLVPVSRVYSSPLCRARQTAKAIAEALGADLATDERLREIDVGKIAGMTWEDTEDE
                     YPKFMEEYRRDPIATRYPGGESVLDVWDRAGHLLRYIAETHRAETVVVVGHAIILKAL
                     ICHILHLDVPNHRRMTLGNASLSVAEVGMQGRGFVGRVIRLNDRHYLEYAGLDTYTGH
                     VDAR"
     gene            complement(4164..>5293)
                     /locus_tag="PLM74_08120"
     CDS             complement(4164..>5293)
                     /locus_tag="PLM74_08120"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HON43077.1"
                     /translation="VSTPEGIAHFLEHKLFEEENGSIDDIFADLGAYSNAFTNHTMTG
                     YLFSCVDNFWPGFDTLLDFVMRPHFTDENVEKEKGIIEQEIRMNEDSPDWQAVNGLLK
                     AMYRLHPVREEIAGTVDSVRRITKEMLYECYRTFYHPSNMTLLVVGDVSCGEVADRAS
                     ARLDSRDFGAAPDVERIRPEEPRDVAIHRVEKSMAVSEPIVCFGYKDPNVSLLGRPLM
                     RRIIGMEMLMHMLAGRSSALYNALYGEGLIDASFDWDYDCEHGYGFAMFEGRSGNPDR
                     LVVRVAEGIAAFLSAGVDPDGFERTRRRVIGSMLRSFDSLEFIAYNFLTHHFRDMRLI
                     ESVDVARSITPDEAEAVARELFDPDLLAVSVIEPGGPESGE"
BASE COUNT         1074 a         1706 c         1371 g         1142 t
ORIGIN      
        1 gcatcctggt cgcgaatgcg gcggtattca atcgaccgtc actcccttca gaatgctcga
       61 ctgtgtttca ctgtatgtca catctagatt attctttgac ctgcctcact tacctgcttt
      121 ctgcgcagcg tccactccgg ccctgaacgc cttcatattg atctccaccg ttccgcgcgg
      181 cacccgcgcc gctatagcct tctcaagcga ctcaggcgac accagtttcg caacggtgtt
      241 cagaacaccc agggccgcta tgtttgcggt tatcgcccta ccagtggcct ccaccgagat
      301 gtcggtgatc gggatcgcgt gcaccacccc acggttttcc ggcacattcg caactaaccc
      361 tgagtccact atgagagttc cgcccggctc tacagtgcac gcgtagcgtt tgcacgcagc
      421 gtccgacatc agcagaagaa catcgggcgc agtcaccttc ggataatcaa tgggctcatc
      481 gcttatgata acctcggcct ttgaggcgcc tccccgcgcc tctgggccat agctctggct
      541 ctgaacggtt tcgtggccgt catgcattgc ggcctcggcc aggatgatgc cggctaggat
      601 caacccctgc ccaccgtatc ccgacagcct tatctcgcgt ctcacttgcc ggccgcctcc
      661 tttgcccgtt cgatcactcc gtcatacagc tcaatgtagc cgggagcgtc gcctcggtaa
      721 aattcgccta tgatgatctt gccctttacc tcagcgggat cgagcttgtc cgcagcggcg
      781 atagttatgg agttcttctt tagcgtctcc accatctcca ccggcccacc caacttattc
      841 ctgcgcccga agtaggtcgg gcagtttcct accgcctcca caaacgcgaa cccgggagtt
      901 gtaatggctc gttcgatgta cgtagacaac tggcgagcat gggccactgt agcgcgggca
      961 acataattgg ctcccgaggc cttcacgagt tcgcacatat cgaagttgcg ctcgacattg
     1021 ccatagggtg ccgtagtagc cagcctgcca ctgggcgtca agggcgagta ttgaccattg
     1081 gtcattccgt atatagagtt gttgaacatg atcgtggtaa ggcccatgtt ccgtcgtgcc
     1141 gcgtgtatca ggtgattccc gcctatcgca gccccgtcgc cgtctccggt cacaacgatc
     1201 acgtgaagat cgggttgtgc gagcttgatc ccggtagcga atgccaatgc tctcccatga
     1261 gtagtatgga gggtgttgaa gtcgagatag cccggagcgc gtgaggcaca accgatgcca
     1321 gacaccacgc acacctggtc cttgggcagg tccagcctgg ctattgccct gagcaatgcg
     1381 ttgagtatga tgccgtgacc gcagccaggg caccagatat ggggcataaa ctgcgtacga
     1441 agatactcat ctattgaccc tgccactcgt cacacctcct ccagtcgcgc gaggatctcc
     1501 tcgggcgtga taggttcgcc gtccactcgc gagtggccaa ttacctcgca cttgccgcgc
     1561 gcagcgcgtt ctacctcccc gacgagctga cccatgttca tctcaggcac tatgatccgg
     1621 tccactcgag ccgctatctc ggccacctgc tcgtctggga acggccatat cgtcttgggc
     1681 tggaacagcc cggccgcgat tcccttctcg cgggctagtc ggactgcacg acgtgccgac
     1741 ctcgctacga tgccgcaggc gactacggcc accctggcgt cgtccatatg ctcgtcccac
     1801 gtgaatgtga tgtcgcgttt gtggttcgtg atcttgccgc agagccgtct gacgagcgcg
     1861 tctaccttag ccggcgcaga cgtgggaaaa ccggtaacgt cgtggtacag acctgttacg
     1921 tgccagcgat agcccgttcc aaaatcagcc atggcaggca ccccgtcggg cccggccgcg
     1981 tacggcaggt agtccgatgg agggactgtc ggacgcggcc gccaagcgat ctcgatttcg
     2041 tcctggcatg gcacaacgac tcgctccctc atgtgggcaa ccacttcgtc cattaagagg
     2101 ataacgggaa ctcgcagcct ttccgcgata ttgactgctt tgactgtaag gtcgaaagct
     2161 tctgaaacgc tagacggata cagggccaca accgggtgat caccgtgagt gccccacctg
     2221 gcctgcatga ggtctccctg ccccggagaa gtcggaagtc ccgtgctcgg ccctgtgcgc
     2281 tggacattga cgatcacaca tgggacttcc gtgaacgaag cgaaccccag gttctcctgt
     2341 ttcagggaga agccggggcc cgacgtagcg gtcatggcct tcataccggc tagcgatgcg
     2401 ccgaccaccg ctgccatgct ggcaatttcg tcttccatct ggatgaactt gccgccgagc
     2461 tgcggcaagc gctctgatag gatctcagcg atctcgctcg acggcgttat ggggtaaccc
     2521 gcgaaaaacc tgactcctgc gagaagagca gcctcagcac aactctcgtt accctgaaga
     2581 agctttaccc tcgtatcgca actatctgcc atcgcgaacc gcctcccttc cttgagcagg
     2641 tgcagcggcc ttcttggcta ccacccgtac tgcgaaatcc ggacacatga tctcacacat
     2701 ttcacacttg atgcagtctt ccggcctagc gactagagcc tttccgtcag gcccagcttc
     2761 gaggaccttc ttcgggcaca tggcgataca tatgccgcaa cctttgcacc actcgcgcct
     2821 cacgagcacc ggcgcctcgg ggtctcctgc caaaaccaat ggactccccc tccacgttca
     2881 atcattgcgt caccgcttgc gcatgagaag cagagcacac acagacgaac ccccgtctcg
     2941 ccgaagtgag ggccgtacat gctctaccct gttaattcta ccatctctgt atgcacgtat
     3001 gcaatgtcaa gtgtatttcg ggtttatcgt atttcatcgg agagggtcag cgttatcggt
     3061 ccgagctctt tgagcaccac cggaaaacgg acctgatggc gaccgggatt aagaacaacg
     3121 cctacagcgt ttacctctag ttccatccct gccggaaggc tgagcggcga ctggtcgctc
     3181 acgtcggacg ctttcacctc agcgcccgct attctgaata tgatgccaga tggcggcact
     3241 gtctcgccat caacttccat ggcgccggcg gctgtcagat gacccgacac cagcgtgttc
     3301 ttcaaccgga acgctagccc tgatcgggag ttcgacatgc ttccgctcac gtagatgcgc
     3361 cttacgacaa cttcaggcac attcatcaag aatcctcctt tgccgggcgg ctgacaaccg
     3421 tcggctgcgc cttcacagcg ctgaatcatc tatacgtcga taggctccgg attgtgaacc
     3481 tgcagtgctc acctggcgtc cacgtgccct gtgtatgtgt caagcccagc atactccagg
     3541 taatgccggt cattgaggcg tatcactctg cccacaaatc ctctgccctg cattcccacc
     3601 tcagccacgg atagcgacgc gtttcccagc gtcatccgtc tgtgattggg cacgtctagg
     3661 tggagaatat ggcatatcag agccttcaga ataatcgcat gcccgaccac aactacggtc
     3721 tccgctcggt gagtctccgc aatgtaccga agcagatgtc cggcgcgatc ccacacgtcg
     3781 agtacgctct cgccccctgg atatcttgta gcaataggat cgcgcctgta ctcctccatg
     3841 aacttcggat actcgtcttc cgtgtcctcc catgtcatgc ctgcgatctt gcccacgtcg
     3901 atctcccgca ggcgctcatc ggtggcaagg tccgccccga gcgcctctgc gatagctttt
     3961 gccgtctgcc gagctctgca cagggggctg gaatagacgc gcgacaccgg aaccaaaggc
     4021 gacgtggcta aatagcttgc acacgccgtt gcctgcgccg acccaaagtc cgataacgga
     4081 atgtccagcc atccctgaaa cctcttctcc ctgttgaaca tggtctcgcc gtgcctcact
     4141 acaagcaatc tggccacggc acatcactcc cccgattccg gcccgccagg ctctatcacc
     4201 gacaccgcca gtagatccgg gtcgaacaac tcccgagcta cggcctcagc ctcgtcaggt
     4261 gtgatagacc tggcaacgtc gacgctctca atcagccgca tgtcgcggaa gtgatgggta
     4321 aggaaattgt aggctatgaa ctctaacgaa tcgaagctgc gcagcatgga tccgataaca
     4381 cgcctgcgcg tccgctcgaa tccatcggga tcaacacccg cgctcagaaa agcggcaatc
     4441 ccctcagcaa cacgcactac aagccggtcc gggttgccgc ttctgccttc gaacatagcg
     4501 aagccataac cgtgttcgca atcgtaatcc cagtcaaagg aggcatcgat cagaccctct
     4561 ccgtacagcg cgttgtacag cgccgaactc cttcccgcca gcatatgcat gagcatctcc
     4621 atgcctatga tcctgcgcat gagaggtcgg ccgagaagac tcacattggg atctttatat
     4681 ccaaaacaga caatgggctc agacacagcc atgctcttct cgacccgatg gatggcgaca
     4741 tctctcggct cctccgggcg gatcctctcc acatcaggag cggcgccaaa atcccgacta
     4801 tccagacgtg ccgatgctct atcagcgacc tccccgcaag acacgtcgcc caccaccagc
     4861 aaagtcatgt tggaggggtg gtagaacgtc ctgtagcatt catacagcat ctccttggtg
     4921 atgcgccgta ccgagtcgac tgttccagcg atctcctcgc gaactggatg cagacggtac
     4981 atcgccttca ggaggccgtt caccgcctgc cagtcagggg agtcctcgtt catcctgatc
     5041 tcctgctcga tgatgccttt ctccttctcc acgttctcgt cggtgaaatg cggccgcatc
     5101 acgaaatcga gaagcgtatc gaaccccggc cagaagttgt cgacacacga gaacaaatag
     5161 ccagtcatcg tgtggttggt gaaggcgttg gagtaggcgc cgagatcggc gaagatgtcg
     5221 tcgatggaac cattttcctc ctcgaatagc ttgtgctcga ggaaatgggc tatgccctcc
     5281 ggtgtcgata ctg
//
LOCUS       DAOTAC010000178         5292 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_13030_length_5292_cov_4.637770, whole genome shotgun sequence.
ACCESSION   DAOTAC010000178 DAOTAC010000000
VERSION     DAOTAC010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5292
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13030_length_5292_cov_4.637770"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..392
                     /locus_tag="PLM74_08125"
     CDS             102..392
                     /locus_tag="PLM74_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006599393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HON43078.1"
                     /translation="MNLAKVVGTIVATQKNPSFSGCKLLICDVCTPDGVLTGSEVVAI
                     DTVGAGENDYVLIVSEGNSSRQAMGKKDAPIDSTVVGIVDRVDTCSSTLVVL"
     gene            720..1382
                     /gene="deoC"
                     /locus_tag="PLM74_08130"
     CDS             720..1382
                     /gene="deoC"
                     /locus_tag="PLM74_08130"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003249061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HON43079.1"
                     /translation="MHRDLAGMIDHTLLKADATEAQVRKLCDEALKYGFASVCVNPTN
                     VALAASILKGSSVRVCTVIGFPLGATTPTAKAVETRDALANGAQEVDMVINVGALKSG
                     NYDVVKRDVHAVVDAAAGQAIVKVILETALLSDEEKIKACLIAKYAGADFVKTSTGFG
                     PGGATVADVALMRRTVGSNMGVKASGGIRDFDTAQKMVAAGATRIGASASVAIVKAAA
                     HD"
     gene            1402..2955
                     /locus_tag="PLM74_08135"
     CDS             1402..2955
                     /locus_tag="PLM74_08135"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HON43080.1"
                     /translation="MTDHDREPIVVAEIEKLIQKASAAQAGLSGFSQQDVDRVCERMA
                     RLGFEGANKLATMAVEETGIGIVRDKVVKNEFATRAVWNHIKNLRTVGFISDDPARRV
                     SEVAVPLGVIAGLLPTTNPTSTAMFKALIAVKSRNSIVFSPHPRAARCSMEAARIMNA
                     AAVEAGAPDGLVSCLETVTMEATDALIHHKLVAAILATGGTAMVRAAYSSGKPAFGVG
                     PGNVPAFIERTANVRQAVADILASKAFDNGTICASEQAIVTESAIEDEVLGELKAQGA
                     HLCSAQEQAALAGVVIRPGGGVNPDVVGKSAPYIASLAGIRVHEGVRALLARYTGVGP
                     EYPLSAEKLCPVLAFYVEKNWEDACARCIELLSYGGLGHSLVIHSGNQAVIREFALRK
                     PVNRILVNTPSSQGAIGLTTGLDPSLTLGCGSWGGSITSDNVGPLHLINRKRLAYHLG
                     GMLVTPEYRGAARGDGCREIGRMGREGGPEDGRALRSRLTGREREVLNSLDDASIVRL
                     VDTALSDMV"
     gene            3012..3305
                     /locus_tag="PLM74_08140"
     CDS             3012..3305
                     /locus_tag="PLM74_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HON43081.1"
                     /translation="MEIALGMIETRGLVGSIEAADAMVKAANVRLIGKEHVGGGFVTV
                     MVRGDVGAVKAAVEAGAAAAAKVGELKSVHVIPRPHSDVEMILPNQGCRPDEK"
     gene            3330..3956
                     /locus_tag="PLM74_08145"
                     /pseudo
     CDS             3330..3956
                     /locus_tag="PLM74_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014452098.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
     gene            4232..>5292
                     /locus_tag="PLM74_08150"
     CDS             4232..>5292
                     /locus_tag="PLM74_08150"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HON43082.1"
                     /translation="MSKVGTRTIDADRIEAAGIVGAGGAGFPTHIKARGAADTVIANG
                     VECEPVLGKDKAIMRAHPDDVVAGLVVMMGCVGAPRGVIAVKRKNSREVDSMRGAARG
                     HANVTVAEVGDYYPAGDEHVLVYEVTGRVVPAGGIPLQVGAVVSNVETLLNVAKAVRD
                     NVPVTRKYVTVAGEVKRPGTFRVPVGTSLAQLVEAAGGVRESLRGGFEVLLDGPMMGK
                     ALPLGQDLSAAQVTKTMSGAIVLPSGHPLLAGRKQSMEHQVRLARSACVQCTHCTDMC
                     PRSLLGHPLRPHLIMRNLGYMRPKLPQDLERYPELMTATLCSECGVCNLVCPMGLTPG
                     HINAGIKRAMAEARLKWKG"
BASE COUNT          983 a         1401 c         1907 g         1001 t
ORIGIN      
        1 cgggaagcca gtatgccgct gccgattccc gtgtcgccgg tggatgcggc gaccgtcggg
       61 atagtggact ccatgacccg taccgtgcga ggtgcccaga ggtgaacctg gcgaaggtag
      121 tgggaaccat agttgcgacg cagaagaatc ccagtttctc cgggtgcaag ctgctcatat
      181 gcgatgtatg cacgcccgat ggggttctta cggggtcaga ggtcgtagcc atcgacaccg
      241 tcggcgccgg cgagaatgac tacgtgctca tagtctccga gggcaactca tcgcggcagg
      301 ccatgggcaa gaaggacgcc cctatcgact cgactgttgt tggaatcgta gatagagtgg
      361 acacctgtag cagcacgctg gtcgttctgt agttttcgaa cagggcaggg cgcttcgcgt
      421 gcggttcgtc tttttggagg tgcttcgggt tggatcttga tgagttaata gcgcgtatca
      481 cgcgagaaat gctgcctacc ctttcagagg cctccaggcc tggagcggga tcgaataccg
      541 gggcaggcgg ctttgcaggc ggcgcccggg cgccgggctt ggccacgagc gaggcctgcc
      601 gaggagcggt gggcgactgc gccggatgcg gcatgtgtgc aggcctgaac gctgcggctg
      661 tggggcaggt tgtaagcgcc ggcgcggaga ggatcagcgc ccatccgggc acgcctgaga
      721 tccatcgcga tctggcgggg atgatagatc acactctgct caaggccgac gccacggagg
      781 cgcaagtgag gaagctgtgt gacgaggcgc tcaagtacgg gtttgcgtcg gtgtgtgtca
      841 atcccactaa tgtggcgctt gcggcgagca ttctgaaagg ctcttcggtg cgtgtttgta
      901 cggtcatcgg gtttcccctt ggggccacga cgccgacggc caaggctgtg gaaacccggg
      961 acgcgctggc caatggggct caggaagtgg acatggtaat caacgtcggg gcgctgaaat
     1021 cgggcaacta cgatgtagtc aagcgagatg tgcatgccgt ggtggatgct gccgcggggc
     1081 aagccatcgt caaggtgata ctggagacgg cattgctgtc tgatgaggag aagatcaagg
     1141 cttgcctcat cgcgaagtac gccggagcgg attttgtgaa gacttctacc gggttcgggc
     1201 ccggaggtgc caccgtggcc gacgtagccc tcatgcgccg cactgtgggc agcaacatgg
     1261 gggtcaaggc atcgggtgga atacgcgact tcgatacggc acagaagatg gttgctgccg
     1321 gggctactag gatcggagcc agcgccagcg tggctattgt gaaggcggcc gcgcacgact
     1381 gaagggggtg ccgacctgtc catgaccgac catgaccggg aaccaatcgt cgttgccgaa
     1441 atagagaagc tcatacagaa ggcgagcgca gcccaggccg ggctgtcggg cttttcgcag
     1501 caggatgtgg accgtgtgtg cgagcgcatg gcccggctcg gctttgaagg tgccaacaag
     1561 ctggccacga tggcagtgga ggagaccggc atcggcatcg tgcgcgacaa ggtggtcaag
     1621 aacgaattcg ccactcgcgc tgtgtggaac catatcaaga atcttcgcac ggtggggttc
     1681 atctctgacg acccggcgcg tcgggtatcg gaggtagctg tgccgctggg agtcattgcg
     1741 gggctcctgc ccactaccaa cccgacgtcc acggccatgt tcaaggccct catcgcggtg
     1801 aagtcaagga atagcattgt gttcagtcct catccgcgcg ctgcacgctg ttcgatggag
     1861 gcggccagga taatgaatgc tgccgcagtc gaggcaggcg ccccggatgg gctggtgagc
     1921 tgcctcgaga cagtcactat ggaggcgaca gacgcgctga tccaccacaa gctggtggcg
     1981 gcgattctgg ctacgggtgg cacggcaatg gtgcgggcag cctacagctc cgggaagccg
     2041 gcattcggcg tgggccccgg caatgttccg gccttcattg agcgcacggc gaacgtgcgc
     2101 caggccgtgg ccgacatcct ggcgagcaag gctttcgata acggcacgat atgcgcctcg
     2161 gagcaggcca tagtgaccga atcggcgatc gaggacgagg tgctcggaga gcttaaggct
     2221 caaggcgccc atctgtgcag tgcgcaggag caggcagcgc tggccggcgt ggtcatcagg
     2281 ccaggcggcg gggtcaatcc ggacgtagtg ggcaaaagtg cgccatacat agctagtctc
     2341 gcggggatac gcgttcatga gggggtcaga gcgcttctgg cacggtacac gggtgttggg
     2401 ccggagtatc cgctgtctgc tgagaaactc tgcccggtgt tggccttcta tgtggagaag
     2461 aattgggaag acgcctgtgc gcgctgcatc gagttgctca gctacggagg cctcggccat
     2521 tcgctggtga tccactcggg taatcaggca gtgatacggg agttcgctct tcgaaagcct
     2581 gtcaacagga tactggtgaa cacgccttcg tcgcagggcg cgattggcct cacaaccgga
     2641 ctggacccat cgctcacact ggggtgtggt tcatggggcg ggagcataac gtctgataac
     2701 gtgggaccgt tgcacttgat aaacaggaaa cgactcgcct accatctagg cggcatgctg
     2761 gtgacgccgg agtaccgcgg ggcggcgcgg ggggatggct gtcgtgaaat cggtcggatg
     2821 gggcgggaag gcggtccgga ggatggccgg gcgctgcgct cgcggcttac tgggcgcgaa
     2881 cgagaggtgc tgaacagtct ggacgatgct tccatcgtcc gtcttgtcga taccgctctg
     2941 tctgatatgg tctagcaaat aagactaata gccccgagca atgcggctgg ggcgggtcag
     3001 gaggaagagc aatggagatt gcactgggaa tgatcgaaac ccgcggccta gttggttcta
     3061 tcgaggcggc cgacgccatg gtcaaggccg caaatgtgag gctcatcggc aaggagcacg
     3121 tcggcggcgg ctttgtcacc gtcatggtac gtggcgatgt cggcgcagtc aaagcagcag
     3181 tagaggcggg agcagctgca gctgccaagg ttggcgagct gaagtcggtg cacgtgattc
     3241 cgaggccgca ttcggatgtg gagatgatcc tgccgaacca gggatgccgt ccggacgaga
     3301 agtaacggct gggtacggag gcattgttca tggcgggcga ggggagtgtg ggagcgggcc
     3361 ggcgtaccga ctggacgcta ttgcgcgctg cgccggtgcc catcggcgtg tctgcaaggc
     3421 atgttcattt gtcggctgag catctgattg ccctatttgg gtcgggccac gaacttgtgc
     3481 cgcgcaaccc gctgtcgcag ccggggcagt ttgctgcgga agagactctg gtcgtggccg
     3541 gccctcgcgg tgcacttgag catgttcggg tgctcgggcc cgtgcggggc aaaagccagg
     3601 tggagctggc gatcactgac tgttacgcca tagggattcc tgcggtagtg cgggattccg
     3661 gcgacgttgc cggttcgccg ggcgttgtgc tagtcggccc cgctgggtca gttgtactgg
     3721 ctgaaggggc gatcgtggcg gcgcgccatc tgcacatgac tacgctggat gcgcggcatc
     3781 tggggctagc cgacggggac tttgtgaccg tgattgcagg agccggcagg cgagccgccg
     3841 tgttcgactc ggtggtgtgc agggtgagtg attcgtaccg gctggagctg cacctcgaca
     3901 cggacgaagc taacgcggcc gggtgccgta gcggcgacat ggcgtggatc gcggacgggg
     3961 cggcgggaac tgcgcgcgca ggcgtcgcgg taggtgcgcc tgtgcctgtg ccggctgtgg
     4021 catccgcgtc ttcgcagatg tccggatctg agatatccgg ttccgcaggc gtgccaatgg
     4081 acttgacggc tcgaatcttg atcactgagg aagacgtact tgaggccgcc cgatcgcacc
     4141 cgcggcgggc gatcgttacg tcggccggcg tgatcgttac gccgcttgct cgtgatgcga
     4201 tcagacgaac cggggtggag ctgatgatcg agtgagcaag gtgggcacgc gcacgataga
     4261 tgcagacaga atcgaagcag cggggatcgt aggggcaggc ggagcgggtt tccctacaca
     4321 tatcaaggca cgcggcgctg ccgacactgt catcgccaat ggggtggagt gcgagcctgt
     4381 gcttggcaag gataaggcta tcatgagggc tcaccctgat gatgttgtag ctgggcttgt
     4441 tgtgatgatg gggtgtgtgg gggcgcctag gggcgtcatc gcggttaagc gcaagaacag
     4501 tagggaagtt gactccatga gaggcgcggc ccgaggccat gccaatgtca ccgtggccga
     4561 ggttggcgac tactaccctg ccggtgatga gcatgtcttg gtgtatgaag tcaccgggcg
     4621 ggtggtgccc gcgggcggta ttccgcttca ggtcggagca gttgtgagca atgtagagac
     4681 acttctcaac gttgcgaagg cggtgcgcga taatgtcccg gtgactcgca aatacgtgac
     4741 ggtagcgggg gaggttaagc gaccgggtac gttcagagta ccggtgggaa ccagtctggc
     4801 tcagcttgtc gaagccgccg gaggggtgcg cgagtcgctg cgcggaggtt tcgaagtcct
     4861 tctggacggc cccatgatgg gcaaagctct gccgctcggt caggatcttt cggcggcgca
     4921 ggtgacgaag acgatgtcgg gtgcgatcgt ccttccgtca gggcatcccc tgcttgccgg
     4981 gcggaagcag tctatggagc atcaggtcag gctggcgcgc tccgcgtgcg tgcagtgcac
     5041 tcactgcacc gacatgtgcc cgcggagcct gctagggcat cctctccgtc cgcacctgat
     5101 catgaggaac ctgggctaca tgcggccgaa actaccgcaa gatctggaac gatatccgga
     5161 gcttatgacc gcgactctct gttcggagtg tggagtgtgc aacctggtct gcccgatggg
     5221 gctcacgcca ggtcacataa atgccggcat aaagcgggcc atggccgagg cgcggctcaa
     5281 gtggaagggg ga
//
LOCUS       DAOTAC010000179         5270 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_13104_length_5270_cov_4.827996, whole genome shotgun sequence.
ACCESSION   DAOTAC010000179 DAOTAC010000000
VERSION     DAOTAC010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5270
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13104_length_5270_cov_4.827996"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="PLM74_08155"
     CDS             complement(<1..650)
                     /locus_tag="PLM74_08155"
                     /inference="COORDINATES: protein motif:HMM:NF014697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HON43083.1"
                     /translation="MIRRMGEFFTRIMEKYLPDAYLFAIILTFISFILALVFTDAGFM
                     GTINAWWGGIWGILAFAMQMTLILVTGHSLALAPPVKRMLAGIARIANTPAKAVFLVA
                     LVAGICSWLQWGFGLIVGGLLAVEIAKRVEGVDYPLLIAAAYAGFLTWHMGISGSTPL
                     TIATVGNPANHIEKLTGQVIPVTQTMFASWNFWPAMFLIVSVAGLLAMAIPTRDKA"
     gene            complement(1011..2396)
                     /locus_tag="PLM74_08160"
     CDS             complement(1011..2396)
                     /locus_tag="PLM74_08160"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HON43084.1"
                     /translation="MDPERILVVDDEPNMAWLFRQAFAGDYEIVAASSGEQALELLRC
                     MDIDLIMLDLRLPGADGIDTLTAARKHGFSGPVIVMTAFGEIKTAVAAMKLGAHDYIT
                     KPFDMDELKLTIESSLRQSRETGKAHDSHRVLEDAVHLRDMITVSPEMRKIFEMVERV
                     SASDASVLVQGESGTGKELMAKAIHYTSPRKNKPFVPVNCAALPESLLESELFGYDEG
                     AFSGARKRKPGRFEIADGGTIFLDEVGDLPLAMQPKILRAAEEKVIERLGGTRRIPVD
                     VRIVAASNRDLRQEVKEGRFRQDLYFRLAVITVSIPPLRDRKEDILVLADYFLQEFCT
                     DLGRPSPLLSNATARALLSHDWPGNVRELKNAMQHLALLCDSNVVEPHSLPGWILLAE
                     PPDPMVSTVDDPLKTQISLKESKRRAFAETEKQRIAEALRIFDGNRTRAAAYLGISRR
                     SLQLKIKRYGL"
     gene            complement(2438..4228)
                     /locus_tag="PLM74_08165"
     CDS             complement(2438..4228)
                     /locus_tag="PLM74_08165"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HON43085.1"
                     /translation="MKSRYLLFQKAEAALIEEKSSKLFGLTRLLDLHLEGDYDGVLLS
                     TAGPQAIHAPASDRIRILNERLAPFTDQLAAAFPGVGVGYYSRDLRAILTYGPSRDFG
                     SKVGTDLGLDHLGWQAMATGKETVAVGSMVRGEIMNCMRPLIRRGRAIGFVWANEALE
                     DIYAQIQVGAGKLFFSPEIEPLLGLTGILLFASKVLLLGKSPSYSGSSVADSRPAGSH
                     MVHRLEQLKCYLELFLNTLSLAVILSDADGHIAFVSRGIQDILGETPASFVSRDIRSV
                     LQCMGIDPHTTLDEFADGSLNRFVNVAIKTHEGERPITMVCTRAEWPMAGPAESDDHS
                     RAGDGHSDGNQFGHIVILEDLKEAQANEERLERAERLAAVGELAAAVAHEIRNPLAVL
                     KGAVSLVPRRLDDREFLEQFSQVATAEMDRINGTVESLLRFSRYSQPHMVSTDIRRIA
                     EKARNLISEYGREMGVAIEFTCPADVPALCGDSDHLTQAILNLLLNGIQAMPAGGTLR
                     VEVKWKPGARYVQIAVADTGMGIAPELRDHVFEMFFTTKESGTGLGLPLVQRIVYNHH
                     GFVEFESETGKGTTFIVRLPVIAQSCPELR"
     gene            4603..5268
                     /locus_tag="PLM74_08170"
     CDS             4603..5268
                     /locus_tag="PLM74_08170"
                     /inference="COORDINATES: protein motif:HMM:NF019947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HON43086.1"
                     /translation="MTSQQSEGYMPAELVDALGRVANGLVATLGSNWEIVVHDFGRLP
                     NSIVAIAGDLTGRTVGGPMTGFGFQLVSSSMENDVLNYPSVTPDGRKLKSSTMFIRDR
                     SGKVIGCVCVNLDLTELMIAQGAIERLCKIDEGERPKEVFARDISEVLNDYLEWTLKQ
                     VDVPVQLMQKEQKLQVIESLERLGVFQIKGAVDIVAQALLSSRYTVYNYLEEVRARNP
                     LRV"
BASE COUNT         1095 a         1474 c         1554 g         1147 t
ORIGIN      
        1 ctggccttat cgcgggtggg gatggccatg gctagaagtc ccgccacgct cacgataagg
       61 aacatggccg gccagaagtt ccaggacgcg aacatggtct gggtgacggg gatgacctgc
      121 ccggtcagct tttcaatgtg gttggctggg ttgcctacag tcgcgatagt cagaggagtg
      181 gaaccggata tgcccatgtg ccacgtgagg aaacctgcgt acgccgctgc gatgagcagg
      241 ggatagtcca ctccctccac acgcttggcg atctcgactg caagcaggcc ccccacaatg
      301 aggccaaacc cccactgcag ccaggaacag attccggcaa cgagcgctac gaggaacaca
      361 gccttagcag gagtattggc tatccgcgcg atgccagcca acatcctctt gaccggcgga
      421 gcgagggcca ggctatgccc tgttaccagg atgagagtca tctgcatggc gaacgcgagt
      481 atgccccaga tgcctcccca ccacgcattg attgttccca tgaaacctgc gtcggtgaag
      541 acgagagcca ggatgaacga gatgaaggtg aggattatcg cgaacaggta cgcatcgggc
      601 aggtacttct ccattatcct cgtgaagaac tcgcccattc gccggatcat gtttaacacc
      661 cccgtcagat tttggtgaaa tcctagagta ttaagtagca agacgtatgc caaggatgaa
      721 ccgggggtgc gccagggacg cattgcgggc gagacccgac ctgagcagag gcttgaggac
      781 agtgcggcag gattctgata gagttttcag gacaatggtg cgaagtgcat tgcgcactct
      841 tgcggtcagg cgtgcaagcc gattcgcagt tggaggcagt acgttggctt tgcaccggct
      901 ttacatgggc tttgcatcgg cttttcgtct gagctgcggt tgctttgtgt ctgcgcaggg
      961 tccgcgatgc gactgcgctg cgtctatgac tatgcctgag cagactgcga ctacagcccg
     1021 taacgcttga tcttgagttg gagtgatcgt ctggagatgc caagatacgc ggcagcccgt
     1081 gtgcggtttc cgtcgaagat tcggagggcc tcagctattc gctgcttttc ggtctcggcg
     1141 aaagctctgc gctttgattc tttcagcgaa atctgagtct tcagtggatc atctactgtg
     1201 gaaaccatag gatctggcgg ctcggccagg agaatccagc ccggcaggct atggggctca
     1261 acgacgttgc tatcgcacag cagggccaag tgctgcatgg cgtttttcag ctcccgtacg
     1321 ttccccggcc aatcgtggga caggagggca cgggcggtag cattgctcag caagggcgac
     1381 ggcctgccca ggtcggtgca gaactcctga aggaagtagt ctgcaagcac taggatgtct
     1441 tccttcctat cgcgaagcgg cggaattgac acagtgatta cggctagtcg gaagtagaga
     1501 tcctgtcgga acctgccttc cttaacttcc tgacggaggt ccctgttgct ggcagccact
     1561 atccggacat ccaccgggat cctgcgtgtg ccgcccagcc gttcgatgac cttctcttcc
     1621 gcggcgcgga gaatcttagg ttgcatagct aggggcaggt cgccgacttc gtctaggaag
     1681 atcgttcctc catccgcgat ctcgaacctt ccgggcttgc gctttcgggc gcccgagaag
     1741 gctccttcat catacccgaa caactcactc tcaagaaggc tctccgggag ggctgcgcag
     1801 ttcacgggca cgaagggctt gttctttcgt ggagaggtgt agtgaatagc tttggccata
     1861 agctctttcc ccgtgccgct ctcgccctgc actaacacag aagcatcact tgcagacact
     1921 ctctccacca tttcgaatat cttgcgcatc tcgggagaga cagtgatcat gtcgcgaagg
     1981 tggactgcgt cttcgaggac tcggtgcgag tcatgcgcct tgcctgtctc cctggactgt
     2041 cggagtgagg actctatcgt caacttgagc tcgtccatgt cgaaaggctt cgtgatgtag
     2101 tcgtgcgctc cgagtttcat ggccgctaca gccgtcttga tctcgccgaa ggccgtcatg
     2161 acgatgacag gtccgctaaa accgtgtttt cgagcggccg taagagtatc gatgccgtct
     2221 gcgcccggca ggcgcagatc cagcatgatc aggtctatgt ccatgcaacg gaggagttcg
     2281 agggcctgct ctccggagga ggccgctacg atctcgtaat cgcctgcgaa tgcctggcgg
     2341 aaaagccacg ccatgttggg ctcatcgtcc acgacgagta ttctttcagg atccaacgct
     2401 gctcacctca cctcagcgca gcgcacatta gcgcacttta gcgcagctca gggcaactct
     2461 gggcaatcac cggaagcctg acgataaagg tagtgccctt tcctgtctcc gattcgaact
     2521 ccacaaaccc atggtggttg tatactatgc gctggacaag gggtagtcca agtcctgtgc
     2581 cgctttcctt cgtggtgaag aacatctcga acacgtggtc gcgcagctcg ggagcaattc
     2641 ccatgcccgt gtcggcgacg gcgatctgta cgtagcgagc gccgggcttc cacttcacct
     2701 ctactcgaag cgtgccgcct gccggcatgg cctgaatccc gttgagcagc aggttgagta
     2761 tggcctgtgt aaggtggtcg ctatcgccgc agagcgcagg cacatctgca gggcatgtga
     2821 actcgatggc tacgcccatc tctctgccat actcggaaat gaggttgcgg gccttttcgg
     2881 ctatgcgccg gatgtcggtg gaaaccatat gtggctgcga gtatcgagag aacctcagca
     2941 gagactcgac cgtgccattg attctgtcca tctccgccgt ggcgacctgc gagaactgct
     3001 cgaggaactc tcggtcatcc agtcgacgcg ggactaggct gacggccccc ttcagcacag
     3061 ccagggggtt cctgatctcg tgagccacgg cggcggccag ctcgcccact gccgcaagac
     3121 gctcagctcg ctccaacctc tcctcgttgg cctgggcctc cttgagatcc tccagtatca
     3181 cgatgtgccc gaattgattg ccgtcgctgt ggccgtcgcc cgcgcggctg tgatcgtctg
     3241 attccgccgg cccggccata ggccactcag ctcgcgtaca taccatggtt atgggccgtt
     3301 ctccttcatg ggtcttgatc gcaacgttca cgaaccggtt gagagacccg tcagcgaatt
     3361 cgtccagtgt agtgtggggg tctatgccca tacactgcaa aacactgcgg atgtcgcgcg
     3421 acacgaagga cgcgggcgtt tcgcccagta tgtcctgtat tcctctggat acgaaggcta
     3481 tgtggccatc agcgtcggac agtatgaccg caaggctgag tgtgttgaga aacagctcga
     3541 ggtagcattt gagctgctcc agcctgtgca ccatgtgtga gccggcgggc cgtgagtccg
     3601 ctaccgatga accgctgtat gagggcgatt tgccgagcag aagaactttg gatgcaaaca
     3661 ggagtattcc cgtgagaccc aggagaggct cgatctccgg agagaagaac agcttacccg
     3721 cacctacctg gatctgggcg tagatatctt ccagcgcctc attcgcccac acgaacccta
     3781 tcgctctgcc ccgccggatc agcgggcgca tgcaattcat gatctcgcct cgtaccatgg
     3841 atccgactgc gaccgtctcc ttgcctgtgg ccattgcctg ccacccgagg tggtcgaggc
     3901 ccagatccgt gcccaccttc gagccgaaat cgcgactggg tccgtaagta agaatggcgc
     3961 gcaggtcgcg tgagtagtag cctacaccga cgccagggaa agcagcggcg agttggtcgg
     4021 taaaaggcgc caggcgctca ttcagtatgc gtatcctgtc ggacgcgggc gcgtgtattg
     4081 cttgaggccc agctgtcgac agcagcacac catcgtagtc accttcaagg tgcaggtcca
     4141 gcaacctggt gagcccaaac agcttgctgc tcttctcctc gatcagcgct gcctcggcct
     4201 tctggaaaag gaggtatcgc gatttcacgg tgtttccctc ctccccggca tacaatgcct
     4261 ctgcatgcag caatgttcta cggaatacgg aggagtcctg ccccggggcg agttctgacg
     4321 ttcggctgcg catactggct tcggcgctga cgccatggta tgtagttgtc aagcaaaatt
     4381 atcgtacact tacagcaaaa ttcgtatatg taggaaggaa gattgtaata cgcgtagaat
     4441 acaatcacca cgggctgctg ggcgcagtgg agcataccga gcgcataccg tacgtcactt
     4501 gagttctagc ttacagggtc gcacagacca gaaggtcggg gcgactcatt cgctccagga
     4561 gtggtcgaga ccggcagacc agtctgaaag gaggatgagt ctatgacatc tcagcagtct
     4621 gaggggtaca tgcctgccga gttagtggac gctctgggcc gagtcgcaaa cggcctagtg
     4681 gctacattag gttcaaactg ggaaattgtg gtacatgatt tcggccgact gcctaattcg
     4741 attgtggcca tagctggtga tctgactgga cgaaccgtgg gggggccaat gacgggtttt
     4801 ggttttcagt tggtctcttc cagcatggag aatgacgttc tgaactatcc aagcgttacc
     4861 ccggatgggc gtaagctaaa gtcatctacc atgttcattc gagaccggtc tggaaaagtg
     4921 atcggctgtg tctgcgtcaa cctggacctc acggaactca tgattgcgca gggcgcgata
     4981 gaaaggctct gcaagatcga tgaaggtgaa cggccaaagg aagtgttcgc aagagacatt
     5041 tctgaggtgc tcaacgacta ccttgagtgg actctgaagc aagtggatgt gcccgtccaa
     5101 ctaatgcaga aggagcagaa acttcaggtc attgaaagtt tggagcgtct tggggtgttc
     5161 cagataaagg gcgctgtcga catagtggct caggcgctgc tctcttccag atacacggtc
     5221 tacaactacc ttgaggaggt aagagcgcga aacccgctgc gcgtgtaagc
//
LOCUS       DAOTAC010000180         5263 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_13128_length_5263_cov_4.770545, whole genome shotgun sequence.
ACCESSION   DAOTAC010000180 DAOTAC010000000
VERSION     DAOTAC010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5263
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13128_length_5263_cov_4.770545"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..944)
                     /locus_tag="PLM74_08175"
     CDS             complement(<1..944)
                     /locus_tag="PLM74_08175"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HON43087.1"
                     /translation="MDTPGKVANLSQVKEFTVDPARRLFSATHDEIINGYTTDLYFVK
                     TRQILDSMGLADTRVTAEIFPRKRGVLAGIDECMNLLQDTDVEVWAMPEGEPFEAKQT
                     VMRIRGKYSEFGIYETALLGILASSSAWATAARDIRDAAGGKPFICFGARHLHPAVAP
                     VMERAAVVGGADGASCILGAKLAGVEPKGTVPHAVFLIAGDTVEVAKVYDREMPPGDA
                     RIILVDTFKDEAEESLRVAEALRERLQGIRLDTPGERGGVTVDLVREVRARLDQAGFS
                     HVKIFVSGGVTPERIVELSAAGADSFGVGSYISGASPI"
     gene            complement(985..1587)
                     /locus_tag="PLM74_08180"
     CDS             complement(985..1587)
                     /locus_tag="PLM74_08180"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HON43088.1"
                     /translation="MLKTLARLIRMVAILAVLGMLASIAVGNPWVQRMLNPIQYQDLI
                     TVYSRRHDLDPFLVAAIMRVESRYQPDAVSSKGARGLMQLMPDTGRWAAGCLGIEGYS
                     DDFLFEPEVNIRIGAWYLRQLLNQFEGNLTVALAAYNGGPSNVEKWLSGGRWSGSIDD
                     LEQIPFGETARYVDRVSRAHRLYLRAYRSRWPGNAPPRVL"
     gene            complement(1704..2315)
                     /gene="coaE"
                     /locus_tag="PLM74_08185"
     CDS             complement(1704..2315)
                     /gene="coaE"
                     /locus_tag="PLM74_08185"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018090359.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HON43089.1"
                     /translation="MIVVGLTGRSGAGKSTVARMFASRGAVCLDVDWVAREVVHPGSP
                     ILAEIERAFGREYLLPDGGLDRRRLGRKVFSDPAALELLNKITHPALVREVDAWITGL
                     AGSSAPPSVAVIDAAVLFESGLTKLTDAVVVVVASEQVQADRISERDGIAVGEALARV
                     RAQRSAEQMLARANFVIRTDCPLEQVEAQVDKVWHLLLSGSCG"
     gene            complement(2390..3025)
                     /gene="ytaF"
                     /locus_tag="PLM74_08190"
     CDS             complement(2390..3025)
                     /gene="ytaF"
                     /locus_tag="PLM74_08190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation membrane protein YtaF"
                     /protein_id="HON43090.1"
                     /translation="MHFIEVMLLSAAISVDALGAGLTYGLRHIRVPVGAALLVAVTSL
                     AGAAVSTLVGSTAGGWLTPLVAERIAGFILVAMGLWIGWEGIRDRRRNNVASGADLLL
                     SLRVRPLGVMIQVMRCPEIADVDRSGQLGAGEAVLLGSALALDTFGTGLAAGLGGASW
                     IIVALAVGMMTLGGFAGGIAVGRRVPEGWGGVWLSITPGVILTFMGLASII"
     gene            complement(3156..5006)
                     /locus_tag="PLM74_08195"
     CDS             complement(3156..5006)
                     /locus_tag="PLM74_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43091.1"
                     /translation="MKKNLTIIAIALLVLAMAIPASAASLSLSGEYSAVFKFDYATGV
                     ETWAPDILGSGADHKLKLNLTFKEGDGIVAYLPLTMTTFSATPTLAIGSWYFSYDTAP
                     WAFWVSQNDAYNAKKFASLGDPLGIVASAKYNGADATKWALNAKGGILGANLNLYALD
                     YDGKAGWVGRATYPLPMDFTVGLVGAYTSLTNTDVVKDDLTFGADVVGKVPGLDATLT
                     LAGAGYWAKTDAWKFEGGTDNAAFLAKISDLKLGPITAWASYTGVGDNFLAHYKTSAA
                     VDDTTILNSYEGSAAAEIGITADLPVGIPTTLTLGDTLWMDYPADPQWNETTGKVVVT
                     PMENLAVTVSGAYKADLDDADAEDHKGYKAHADVKYTAFGLDLKPFVDYKVDSYANQT
                     PHGDPAKIVDTIVGLNIDGSPLAGLDVGVRASYTIEEPKTALKGWGIYATELNPGFVK
                     SAMTKIAGVAELNKVGDLDATTDFYGYAGSDIVVTDQLTAKVGVLTKDEKEKIAAHAA
                     LTYKVSDSIATGLAYTYRGFAVAPDADHENMWKPFDDKNQSYVKANVTGTVGKSTIEL
                     AYGVSGLQSKTCATLGCDDFHVGKPWDWLYHVPGTYMNWELLTLTVKVPF"
BASE COUNT         1052 a         1706 c         1443 g         1062 t
ORIGIN      
        1 tctataggag acgcgccgga gatgtagctg cccacgccga acgagtcggc tcccgcagcc
       61 gacagttcca ctatacgctc gggggtcacg cctcccgaga cgaatatctt gacatggctg
      121 aacccggcct gatccaggcg ggcgcgcact tcgcgcacta gatccactgt taccccgccc
      181 cgctcgcccg gggtgtcgag ccgaatgccc tgcagtcgct cgcgaagcgc ctccgcgacg
      241 cgcagtgact cctcggcttc gtctttgaat gtgtcgacca ggatgatccg agcgtctccc
      301 ggaggcatct cacgatcgta caccttggca acttccacag tgtcgccggc tatcaggaag
      361 accgcatggg ggactgttcc cttaggttcg acgccggcta gtttcgcgcc gaggatgcag
      421 ctggcgccat cggcgccgcc caccactgca gcccgctcca tcaccggagc gaccgccggg
      481 tggaggtggc gcgcgccaaa gcatatgaac ggcttgccgc ctgccgcgtc gcgtatatct
      541 cgggccgctg tagcccacgc gcttgagctg gcgagtattc caaggagtgc agtctcatat
      601 atgccgaact cggagtattt gccccggatt cgcatgacgg tctgcttggc ttcgaacggt
      661 tctccctcgg gcattgccca tacctcgaca tccgtatcct gtaggaggtt catgcactcg
      721 tcgatccccg ccagaacgcc acgtttcctc gggaatatct cggccgtaac ccgagtgtcg
      781 gcgagcccca tcgagtccag tatctgccgg gtcttcacaa agtacagatc cgtagtgtag
      841 ccgttgataa tctcatcgtg cgtcgccgag aagagacgtc gggctggatc cacggtgaac
      901 tccttcacct gcgagagatt agcgaccttg cctggagtgt ccatctacac aactaccccc
      961 cggacaattc ccattacaga gtgattatag cacacgtggc ggcgcattgc cgggccaccg
     1021 tgatcgatag gccctgaggt agagcctatg tgctctactc acccggtcca cgtatcgggc
     1081 agtctcccca aacggtatct gctccagatc gtcgatgctc cccgaccatc ttccgccgga
     1141 tagccacttt tccacattgc tcgggccacc gttgtaggct gccagagcta cagttaggtt
     1201 gccctcgaac tggttcagaa gttgcctgag ataccatgcg cctatcctaa tgttgacttc
     1261 gggctcgaac aggaagtcgt cggagtaccc ttcgatcccc aggcacccgg cagcccaccg
     1321 cccggtatcc ggcatgagct gcatcaaacc ccgggcgccc tttgacgaca ccgcatctgg
     1381 ctggtaccgg ctctccactc tcatgatagc ggccacgaga aaaggatcta ggtcgtgccg
     1441 gcgcgagtac acggtgatca ggtcctggta ttgaatgggg ttgagcatgc gctgaaccca
     1501 cggattaccg accgctattg aggccagcat gccgagcaca gccagaatcg ccaccatccg
     1561 tatcaacctc gcaagcgtct tcagcatctc accaactccg actacaccca tatgccgatg
     1621 cagcgtgttt catcatgcgc cgaatcatct tttcctacgc ttcgggttcg cagtgcaggc
     1681 gactgctcgc gagcaggtca cggctatcca cagctacccg atagcaacag gtgccacacc
     1741 ttgtccacct gcgcctcgac ctgctccagc gggcagtcgg tgcggatcac aaaattggca
     1801 cgagccagca tttgctcggc cgatcgctgc gcccgcaccc gggcgagcgc ctcgcccacg
     1861 gcaatgccgt cgcgctcgga aatcctatcg gcctgcacct gttcactggc cacaacgact
     1921 actaccgcgt ccgtgagctt ggtcaatccc gactcaaaaa gcaccgccgc atcgatcacc
     1981 gccacagacg ggggtgcact gctgcccgcg agtccagtta tccaggcgtc cacctcgcgc
     2041 acaagggctg ggtgggtaat cttgttcaga agctccagtg cagccggatc cgagaagacc
     2101 tttcggccaa gcctacgccg gtcaagcccg ccgtcaggca ggaggtattc gcgaccaaag
     2161 gccctctcga tctcggccag aataggcgag ccggggtgga ccacttccct ggccacccaa
     2221 tcgacatcga ggcagaccgc tccccgactg gcgaacatgc gtgccaccgt actcttgccc
     2281 gccccgcttc tccctgtcag gcccacgact atcatgtcca cccctcccgg ctgccgccgt
     2341 gcccggcact gagccgacat gcgcaaggag cccaataccg cacgagccgc tatatgatcg
     2401 aagccaggcc catgaatgta agtataacgc ctggagtgat actgagccat accccgcccc
     2461 atccttcagg cacacgccgc cccactgcta tgcctcctgc aaaaccgccg agtgtcatca
     2521 tccccactgc gagagctaca atgatccagc tcgccccgcc tagccctgcc gcaaggccgg
     2581 tgccaaatgt gtccagggca agagcggacc cgagcagcac ggcctcaccc gcgcccagct
     2641 ggcccgaacg gtcaacgtcc gctatctcgg ggcaccgcat gacctgtatc atcaccccaa
     2701 gcggccttac acgcagcgac agcagaagat cagctccact ggccacgttg ttgcgccttc
     2761 gatccctgat tccctcccag cctatccata ggcccatggc cacaagaatg aaaccggcaa
     2821 tcctctcggc aacaagcgga gtcagccatc cgcccgctgt cgaacccaca agagtggaca
     2881 ccgccgcccc ggcaagtgac gtaacagcca ccagcaacgc cgcgcccaca ggcaccctaa
     2941 tgtgacgaag cccataggtc aagccggccc ccagggcatc aacgctgatc gcagccgaaa
     3001 gtagcataac ctcaatgaag tgcatgttcg tcctccagct ccgccgcgct gtgcgcatag
     3061 cccttcagca ctcgtcggag ccagagtcag tatacgaacc cggcatagaa aaggaccccc
     3121 ggattgctcc ggaggccctt gttcgttcgt tcagcctaga acgggacctt gaccgtcaag
     3181 gtgagtagct cccagttcat gtaggtgccg ggaacgtggt acagccaatc ccacggcttg
     3241 ccgacgtgga agtcgtcgca tcccagagtg gcgcaggtct tgctctggag accgctgaca
     3301 ccgtaggcca gctcgatggt gctcttgcct accgtaccgg tgacgttagc cttcacataa
     3361 ctctggttct tgtcgtcaaa gggcttccac atgttctcgt gatcagcatc cggcgccacc
     3421 gcgaaaccgc gataggtgta agcaagaccg gtcgctatgc tatcgctcac cttgtaggtc
     3481 agggcagcat gtgcggcgat cttttccttc tcgtccttcg tcagcacgcc aaccttcgcg
     3541 gtcagctgat cggtgacgac gatgtcagat ccagcgtagc catagaagtc agtggtcgca
     3601 tcgaggtcgc caaccttgtt aagctccgcc actccggcta tcttggtcat ggcgctcttc
     3661 acgaagccag ggttgagctc ggtggcgtag atgccccagc ccttgagagc cgtcttgggc
     3721 tcctcaatgg tgtagctagc cctgacgcca acgtccagtc cggcgagcgg gctgccgtcg
     3781 atgttgagcc caacgatggt atcaacgatc ttggcaggat ccccatgggg agtctggttg
     3841 gcataggagt cgaccttgta atcgacgaac ggcttcaggt caaggccaaa ggcggtgtac
     3901 ttgacatccg catgagcctt gtagcccttg tggtcctctg catcggcatc gtcaaggtct
     3961 gccttgtagg cgccggagac ggtgaccgca aggttctcca taggagtgac gactaccttg
     4021 ccggtggtct cgttccactg cggatccgca gggtaatcca tccacagggt atcgcccagc
     4081 gtcagcgtag tcgggatgcc aaccggcaga tctgccgtga tcccgatctc agcagcagcg
     4141 ctaccttcgt agctattcag gatagtcgta tcgtccactg ctgcactggt cttgtagtgc
     4201 gctaggaagt tgtcgccaac acccgtgtag cttgcccagg cggtgatggg acccaacttc
     4261 agatcgctga tcttggcgag gaaagcagca ttgtctgtac cgccttcgaa cttccaggca
     4321 tccgtcttcg cccaataccc agcgccagcc agggtaaggg tggcatccag gccaggaacc
     4381 ttgccaacta cgtcagctcc gaaggtcagg tcatccttga ccacatccgt attagtgagg
     4441 ctggtgtatg cgccaactag cccgacggtg aagtccatcg gcagaggata tgtggccctt
     4501 ccgacccaac ctgcttttcc atcatagtcg agggcataca gatttaggtt ggccccgaga
     4561 ataccgccct tggcattcag tgcccatttg gtcgcatctg cgccattgta cttcgcggaa
     4621 gcaacgatgc ccagcgggtc gccgagcgaa gcgaacttct tggcgttata tgcgtcgttc
     4681 tggctgaccc aaaatgccca cggagcagtg tcgtacgaga agtaccagct gcctatagcg
     4741 agcgtgggag ttgctgagaa ggtggtcatg gtaagcggca ggtaggccac aatgccgtcg
     4801 ccctctttga acgtcaggtt gagcttcagc ttatggtctg cgccgcttcc cagaatgtca
     4861 ggtgcccaag tctcaacgcc tgtagcatag tcgaacttga acacagcgct gtactcgcca
     4921 ctcaggctca ggctcgccgc tgaagcgggg atcgccatgg ccaataccag gagggcgatt
     4981 gcgatgatag tgaggttctt cttcatgaaa gatccccctt atggtttttt gtagtttcac
     5041 gctttccgtt ggccgtcagg gggagagctt gtgagtgtcc aagtttcctc ataatcttcc
     5101 ggcgtcgggc ctctgttcag cgtgttgcgg ctctgtcgcg tatgccgcga tccggccgct
     5161 atagactcga tcctaagagc aaaccagggg tgcacctcct ttcgggcttt caggatcgaa
     5221 aggtatatcc agactgcaat gtgatactag cgaagcgctg gga
//
LOCUS       DAOTAC010000181         5258 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_13147_length_5258_cov_4.145109, whole genome shotgun sequence.
ACCESSION   DAOTAC010000181 DAOTAC010000000
VERSION     DAOTAC010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5258
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13147_length_5258_cov_4.145109"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="PLM74_08200"
     CDS             complement(<1..693)
                     /locus_tag="PLM74_08200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HON43092.1"
                     /translation="MRVAVVTDSMADLPKEIAEEHGVSVVPLTVIFGEETFLDGVDMT
                     SRDFFARLKTSSVMPSTSQPSPADFIQIYDDLLKTNDHIVSVHASHKLSGTYQSACIA
                     RDTLESHAIDVIDSKQVTMCEGFAALEAARAAQRGASPEEICEAALRVAGSVQLLFTV
                     DTLEFLERNGRIGKAASLLGTLLSIKPIIHLEDGEVAPRDKVLGARRLLPRVMELMDE
                     AIDSRAIHAAVLH"
     gene            complement(690..1682)
                     /locus_tag="PLM74_08205"
     CDS             complement(690..1682)
                     /locus_tag="PLM74_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008518450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HON43093.1"
                     /translation="MRGYIGVDVGSVSTNLVLLDESGSLVDSSYLRTRGSPIEAVKEG
                     MRALRDAHRDFEVAAAGATGSGRKLAGVVIGADVVKNEITAHAVAAMAVVPGVRTVIE
                     IGGQDSKIIVLRSGIVVDFGMNAVCAAGTGSFLEHQAQRLGVPIEDFGRIACQSSSPV
                     RIAGKCAVFAESDMVHKQQVGYSLPDILRGLCEALVRNYLSNVGKGKAVAPPAVFQGG
                     VAANLGMRRAFEDALGFEVIVPPHFDVMGAIGAAMLAQRWAASGGETTAFAGWDVPEQ
                     VYSTVSWECDGCPNTCEIVEVARDSKAVARWGGRCGRWERLEQDEEHTERVGSG"
     gene            complement(1795..3021)
                     /locus_tag="PLM74_08210"
     CDS             complement(1795..3021)
                     /locus_tag="PLM74_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008195551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HON43094.1"
                     /translation="MRYRQFGRCGFEVSVLGFGCMRLPCVSGDQSAIREDEAVRMVRY
                     AIDEGVNYVDTAYGYHGGNSERLVGRALADRYRDRVKLATKLPVWLCKSYDDFDRFLS
                     EQLERLATDHIDCYLLHSLNKKSWSKVRDMGVLDFLAEQLELGRIRYAGFSFHAAYEV
                     FRDIVDAFDWDFCQIMLNYVDVEYQAGLAGLKYAAERGLAVVVMEPLRGGRLAANLPD
                     EVVAALGAVSVDSAGRAGRAGSATGAVGTAGETSDGSVGSEGWSPAEWALRWVWNLPE
                     VSVALSGMSNMDQVVDNIRIVSDAAPGALTERQLAMLARAREAYMARTKIACTECGYC
                     QPCPQGVLIPDVFGLYNEAEIFNAWESSRWMYGGIIKAAKDASRCVECGQCEAACPQQ
                     LRVIELLKEAHAALAE"
     gene            3304..5184
                     /locus_tag="PLM74_08215"
     CDS             3304..5184
                     /locus_tag="PLM74_08215"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HON43095.1"
                     /translation="MSLFTPIMPVRGHLKRRLTAAALLALTPTLALAGMLALNEFVGS
                     CSGVTARNTAEARHLGTALSSHLNSLLDRQALLLDTCGASQAEPSPQMHMMDKLMTDL
                     RSQWIIGCSVFSLNGQLLCTAGVAPSYSPLFDTSLLEPVLSSSKPTTCFIATQEEDGS
                     DAIPRVVLANRVQGGKREPRVFAMEVDVDWMARLAQSVWDDGSGPDVCGFVDPSGQVL
                     LWDRNGSSQPISKSSESLNAKWPLACDEAATMVFTHRGAWYGEALSGSYRVSAVAIPW
                     LGWTSIAATRNDPASAMAHYVRLKHVLSMVFTALAGLLAAARLVSGVSAEACALERAA
                     IAVGAGDLSARSGLRGATDVGAAGRAFDEMAISLAELEQSRLQALQVASHELRNPLSA
                     VKGAAALLSMRIDDAKQASELRPLVDIIVRQANDLTDKTIRIFDALILANHWRAMDKK
                     PVELRELTREALRPFLAAESGNRLAVCGLDRDLPDLVVIGDREQLETVIASLVSNGLK
                     YSMGEGIVRIAVRPTEGAARISVCDRGIGVPDAELSSIFDGFARGSNLEGRDPGGLGL
                     GLYVSAMIIRQHGGRIWAESDGQTGTHFHVELPLHPVVLSTRNQNRGDENGRNTHSGG
                     RG"
BASE COUNT         1044 a         1717 c         1443 g         1054 t
ORIGIN      
        1 atgcagcacc gcagcgtgaa tggcgcgcga gtcgatcgct tcatccatta gttccatgac
       61 tctgggaagc agacgccgcg ctcccagcac tttgtctcgc ggggcaactt cgccgtcttc
      121 aaggtggatt atgggtttga tcgacagcag cgtgcccagc agcgatgcag ccttgccgat
      181 cctcccgttg cgctccagaa actcgagagt atctacggtg aaaagcagct gcacagagcc
      241 tgcaacccgc agtgctgcct cgcagatctc ctcgggactc gcccctcgct gcgccgcccg
      301 ggcggcctcg agcgcagcga acccttcgca catggttacc tgtttggagt cgattacgtc
      361 tatcgcatgg ctttccagag tatcccgggc aatgcaggcg ctctggtatg tgccgctcag
      421 cttgtgcgaa gcgtgcaccg acactatgtg gtcattcgtt ttgagcaggt catcatagat
      481 ctgtatgaag tccgccggcg aaggttggga cgtagacggc atcacactgg acgtcttcag
      541 tctggcgaag aagtcgcgtg aggtcatgtc tacgccgtcc agaaaggtct cctcgccgaa
      601 gataactgtc agaggcacta cggacactcc atgttcctcg gcgatctcct tgggcaagtc
      661 agccatgctg tcagtaacaa ccgcaactct catccggagc ccaccctctc tgtatgctct
      721 tcgtcctgtt caagcctttc ccatctgccg caccgaccgc cccagcgcgc caccgccttg
      781 gagtcgcgcg cgacctcgac aatctcacac gtgttggggc agccatcgca ctcccacgac
      841 accgtagagt acacctgctc cggcacgtcc cagcctgcga aggctgtggt ttcgcctcct
      901 gacgccgccc aacgttgcgc cagcatggcc gcgcctatgg cacccatcac gtcgaaatgc
      961 ggcgggacga ttacctcgaa acccagggcg tcttcaaagg ccctgcgcat tcccaggttg
     1021 gcggcaacgc ctccctgaaa tacggcgggc ggagccacag ccttcccctt acccacgtta
     1081 ctcaggtagt tgcggactaa tgcttcgcac aggccgcgca ggatatccgg tagtgaatat
     1141 cccacctgct gcttgtgaac catgtccgat tcggcgaaca ctgcgcactt ccctgcaatc
     1201 cttacaggcg aggaagactg acatgcgatt cggccgaagt cctcgatggg cactccgagc
     1261 cgctgtgcct gatgttccag aaaagacccg gtccctgcag cacagaccgc attcattccg
     1321 aagtccacta caatgcccga gcgcaggact atgatcttgg agtcctggcc tccgatctct
     1381 atgacagtac gtacgccagg gacaaccgcc atggccgcaa ctgcgtgtgc tgtgatctcg
     1441 ttcttcacca cgtcggcacc tatgacgact ccggccagct tccgtccact gcctgtggcg
     1501 ccggcggcgg ccacttcgaa atcccggtgc gcatccctca gcgcccgcat gccttccttt
     1561 actgcttcta tcgggctacc gcgcgtcctc agatatgaag agtcaaccaa agagcctgat
     1621 tcgtccagca gcacaagatt ggtgctgacg gaccctacgt ccactccgat gtagccacgc
     1681 accctgtgca ccccccatcc ccagagggtg cccagtcgca gccaaacaga ttcgcgagcc
     1741 cgcgccctgc gccggtggcc ggtggcaccg cacttcagct tgccgcagcc cgcctcactc
     1801 agccagcgcc gcgtgggcct ccttcagcag ttcgatcacg cgcagttgct gcggacatgc
     1861 cgcctcgcac tggccgcact ctacgcacct ggacgcatcc ttggcggctt tgatgatccc
     1921 cccgtacatc cacctcgaac tctcccaggc attgaagatc tcagcctcat tgtagaggcc
     1981 gaatacgtcg gggatcagca caccctgcgg acacggttgg caatacccgc actcagtgca
     2041 tgcgatcttg gtccgcgcca tgtaggcctc tcgcgcccgc gcaagcatcg ccaactgccg
     2101 ctcggtcagc gctccgggag cggcatccga aactatgcgt atgttgtcca caacctggtc
     2161 catattgctc atcccgctca gggccacgct gacttcgggc aggttccaca cccagcgcag
     2221 agcccactcc gccgggctcc agccctcact accgacactg ccgtcactgg tctcgcccgc
     2281 cgtgccaact gcgcctgttg cgctgcctgc tctgcctgct ctgcctgcac tgtccacact
     2341 gaccgcgcca agcgccgcga ctacctcgtc cggcaagttc gcagcaagcc ggcctccccg
     2401 caaaggttcc attacaacca ctgccagccc acgctctgct gcatacttca gcccggccaa
     2461 tccggcctgg tattctacgt ctacgtagtt gagcatgatc tggcaaaaat cccagtcgaa
     2521 agcatccact atgtctcgaa acacctcata ggcagcatga aaggagaatc cagcgtatcg
     2581 tattctcccc agctccaact gctcagcgag gaagtccagc actcccatat cgcgcacctt
     2641 cgaccacgac ttcttgttga ggctgtggag caggtagcag tcaatgtgat ccgtggcgag
     2701 gcgttcgagt tgctcgctaa ggaaccggtc gaagtcatcg tacgacttgc acagccacac
     2761 cggaagtttg gtggcgagct ttaccctgtc acggtatcgg tcggcaagag cccggcccac
     2821 gagcctttcg ctgttgcccc cgtggtatcc gtaagctgta tccacgtaat tgacgccctc
     2881 atctatggcg tatctcacca tccgcactgc ctcatcctcg cgtattgccg actgatcgcc
     2941 gctaacgcaa ggaagtcgca tgcatccgaa accgaggaca gatacttcga acccacaccg
     3001 cccgaactgc ctgtatctca caacccatcc tccctatatc cagtattgcc gtaaccgtca
     3061 ttccatcctt tgtgtcttgt cattttctcc ttcagccatg cacatccctc cctgatgctc
     3121 cccttctcac acgcccagcg gcaggttcga tagcacgctg cagccaaacc gcgatttacc
     3181 tcgaatgaca tcgtaaagtg acgcaatagc acaagaggtg agtggtccgg gaatgtcgta
     3241 gtattctcat caaataagtg ccctttcgag ttcgaattgc agtattcgat ggaggacgcc
     3301 tcgatgtcgc tatttacgcc gatcatgcct gttcgcgggc acctcaagcg aagactgacc
     3361 gctgctgcgt tgctcgcatt gactccgaca ctagccctag ccggcatgct ggcactgaat
     3421 gagtttgttg gatcctgctc gggcgtgacg gcacggaaca cggcagaggc ccgccactta
     3481 gggactgctc tctcatccca tctgaacagc ttgcttgatc ggcaagcgct actactcgac
     3541 acctgcggcg catcccaggc agaacccagc ccacagatgc acatgatgga taagctgatg
     3601 accgacttgc gctctcaatg gatcatcgga tgctcggtgt tcagcctcaa cgggcagctt
     3661 ttgtgcactg ccggcgtcgc accgagctac tctccgctgt tcgacacgtc actgcttgag
     3721 cctgtgctat caagcagtaa gcctaccacc tgcttcatcg cgacacaaga agaagacggc
     3781 tccgacgcta tccctcgagt cgtcctggct aaccgagtcc agggcggaaa gcgcgagccg
     3841 cgcgtgtttg ccatggaggt tgacgtggac tggatggcga gattagccca gagcgtgtgg
     3901 gatgatggtt ccggcccgga cgtgtgcggg tttgtggacc cgagcggcca agtgctgctg
     3961 tgggaccgca acggatcgag tcaaccaatc agcaagtctt ctgaatcgct gaacgccaaa
     4021 tggcctctgg cttgcgatga agccgctacc atggtcttca ctcatagagg cgcctggtac
     4081 ggcgaagccc tttcaggaag ctaccgggta tccgccgtcg ccatcccctg gctgggatgg
     4141 accagtatcg ctgcgacaag gaacgatccg gccagcgcca tggcacatta tgtcaggctg
     4201 aagcacgtgt tgtccatggt cttcactgcg ctcgccgggc tgctggcggc agctaggctg
     4261 gtgagcggcg tctcagccga ggcatgcgcc cttgaacgtg cagccatcgc cgtaggcgcg
     4321 ggcgacctct ctgcccgatc cgggctccgc ggcgccaccg atgtaggcgc tgcaggtcgc
     4381 gcctttgatg agatggctat ctctttggca gagttggagc agtcgagact ccaggcgctc
     4441 caggtggcct cgcatgagct gcgcaatccg ctgtccgcgg tgaagggagc agcagcattg
     4501 ctcagtatgc gcatagacga cgccaaacag gcctccgagt taaggccgct ggtagacatc
     4561 atagtgcgcc aggcaaacga cctgaccgac aagacgatac gtatcttcga cgccctcata
     4621 ctggccaacc attggcgcgc catggacaag aagccggttg aactcaggga gctgactcgc
     4681 gaggcgcttc ggcccttcct ggccgccgaa agcggtaacc ggcttgccgt gtgcggcctc
     4741 gatcgcgacc tgccggacct agtggtcatc ggagaccgcg agcaactcga gactgtgatc
     4801 gcaagtctgg tctcaaatgg actgaagtac tcgatgggcg agggaatagt gcgcatcgcc
     4861 gtgaggccca ccgagggcgc agcgagaatt agtgtatgcg accgcggaat aggcgtgccc
     4921 gacgccgagc tgtcctcgat cttcgacggt tttgcccgcg gcagcaacct ggagggacgc
     4981 gatccaggtg ggcttggcct ggggctctac gtgtccgcga tgataatcag gcagcatgga
     5041 ggtaggatct gggcagaaag tgacggacag accggcacgc acttccacgt ggaactccca
     5101 ctccatccag ttgtcctctc aacacgcaat cagaacaggg gggatgagaa tggccggaat
     5161 actcatagcg gaggacgagg ctgacatcgc catgatcctc cgcgagatac tggagatggc
     5221 cggacatcat gtgaccctgg ctatcgatgg cgcgatgg
//
LOCUS       DAOTAC010000182         5257 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_13154_length_5257_cov_4.315648, whole genome shotgun sequence.
ACCESSION   DAOTAC010000182 DAOTAC010000000
VERSION     DAOTAC010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5257
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13154_length_5257_cov_4.315648"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="PLM74_08220"
     CDS             <1..237
                     /locus_tag="PLM74_08220"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HON43096.1"
                     /translation="CYGVAQSARDRFGSLVAVGIATIMLVHIFVNVGMTMNVSPCTGI
                     PLPFFSAGGSSLIATMLGVALLQSIHMRRKKIVF"
     gene            317..1189
                     /locus_tag="PLM74_08225"
     CDS             317..1189
                     /locus_tag="PLM74_08225"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HON43097.1"
                     /translation="MHVEITVDPLVVVFAVLYALAGSLWQWAVVFASLLVHEVSHAVV
                     AVGFGFAVSEVRITPIGAAVRVDDTIQLRAEAEAAVAMAGPMTSLVLAGAGYILLAYG
                     KPDTAAAEFFIGANIVLAMLNLLPAFPLDGGRVLRALLVERMGMSGATRAAVNVSRWI
                     GGLAVVAGAVGAATGALSPLVAAMGVLIWVEAGREVRFAPYAAMRSAMRKRAGLARNG
                     FAPAALIAASPDMSAGAVLGRLSGQQFAIISVVAPDGREMGRLSEIELMDGVCARGPG
                     VNLETLVRGSKHRW"
     gene            1183..3015
                     /locus_tag="PLM74_08230"
     CDS             1183..3015
                     /locus_tag="PLM74_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015758749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HON43098.1"
                     /translation="MVNKELLATEILPKVSQPARYTGHELNAGAGYRAGAEAALLLAF
                     PDVYEVGMSYIGFKILYDIVNSNPVWAVERAFAPWPDMEAQMRLHGIPLYGLETYTPA
                     RSFDLIGFTLQYELTYSNVLCMLELAGIPARADRRGQGDPIVIAGGPCASNPEPIAPF
                     LDLVVVGDGEEAIVEIMSETARGKREGASREAILARLAKIAGVYWPRAQNHVTRRIVA
                     DLDSAQYPRKFVVPFLEAVHDRVALEVMRGCSRGCRFCQAGMVYRPVRERSMNDLCEL
                     AEALVSSSGYEELSLLSLSSADYTRIKELISRLAAEYGPQGVAVSLPSLRVDSFSIAL
                     SRDVEKSRRTGLTFAPEAGTQRMRDIINKGVTEDDLISAATDAFESGWDQIKLYFMIG
                     LPYETFEDIDGIAALSRRVLQLGRQVAGARGKAGRARVNVSVSSFVPKPHTPFQWFAQ
                     NTREQLEQKQSFLKERMRARGLSVSFHDVWASHLEAAFARGDRHLAPVIERAVALGCR
                     FDGWSDQFRPDLWQRAFTDMGLDSSMWANAEYGFDDSLPWDHIDMGVSREFLVREARR
                     AESGLTTPDCREAGCSGCGVCRGDVRVRLAGAFAGSSSQGGGQA"
     gene            3012..3761
                     /locus_tag="PLM74_08235"
     CDS             3012..3761
                     /locus_tag="PLM74_08235"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HON43099.1"
                     /translation="MSAAVVRVRLGKGDEIRFVSHLGFMRAFERTVRRARIPVAYSGG
                     YHPHPKISFATALGVGMTSEAEYLDMELAPADSCDLSTQDVFERLQPTAPPGMPVIAV
                     CAAEGGPALASRVEWASYRVDVHADARPELEDAVLEFLSSDSAIAKVNTKSGQRRTDI
                     RPMVREIRWSGSGALECTLACGGRAHLRPQDFIAHLDSMRGRGDAPMLGHESIGPSRI
                     HRTGLYFTDREGGDLLSPMNVECKDLVMAWS"
     gene            3781..>5257
                     /locus_tag="PLM74_08240"
     CDS             3781..>5257
                     /locus_tag="PLM74_08240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HON43100.1"
                     /translation="MAREIIVNSESGEVRAAVLEDGVLVDLFIERSLHPRYAGNIYKG
                     TVENVLPGMQAAFVDVGLERNVFLYVDDALAGRNGREARIVREADGEEITVPNKKQRS
                     IKDILKPGQEIIVQVSKEPIGTKGARVVTDITLPGRYVVLMPTVDYVGVSRRIDEDGE
                     RDRLKKIAQAAKPKRMGVIVRTVAEGLSQEEIASDIQFLVKLWRRIQARGRRAKPPAL
                     LHKDYDLVFRLVRDHFAEDVTQFVVDDPDDLKKVNDLVKMFPEPMRDRVHLYTGQEPI
                     FDSYGLEEEIARALRRKVWLACGGYLVIDHTEALTVIDVNTGKFTGSTCLADTVLKTN
                     LEAASEIARQMRLRNIGGIIIADFIDMDDENHQKQVVRKLEESLARDKTKASVLGFTQ
                     LGLVEMTRKKVQQGLAESMMKVCPMCDGRGRILSEETLAFRAMRAIKKEALSTSQPAM
                     LVLLHPSVAAVLIGSGGSNLAALEEETGRAIYVKGSFDQKVE"
BASE COUNT         1022 a         1425 c         1770 g         1040 t
ORIGIN      
        1 tgctacggtg tagcccagtc cgcccgcgac agattcggca gcctggttgc cgtgggaatc
       61 gcgactatca tgcttgtcca catcttcgtg aatgtgggca tgaccatgaa cgtgtcgcca
      121 tgcaccggca tccctctgcc cttcttcagc gccggaggga gctccctcat agcgaccatg
      181 ctgggagtcg cgcttttgca gagtatccac atgaggagaa agaagatcgt cttctagacg
      241 caggggcgtg ccactataat ctcataggaa tcccggagct tcgggcggag ccggagggga
      301 ggcgtccatg gggcggatgc acgtggagat caccgtcgac ccgcttgtcg tggtattcgc
      361 cgttttgtac gccctcgcgg gcagtctgtg gcagtgggcg gtggtgtttg cctcgctttt
      421 ggtgcacgag gtctcccacg ccgtggtcgc agtcggtttc gggttcgccg tttctgaggt
      481 gaggatcacc cccatagggg cggccgtccg tgtcgacgac acaattcagc ttcgggcaga
      541 ggcggaggca gcggtggcca tggcgggccc catgacatcg ctggttctgg caggcgcagg
      601 ctatattctt ctggcatacg gtaaacctga tactgcggct gccgagttct tcatcggcgc
      661 caatatcgtg ctcgcgatgc tcaatctgct gccggccttc ccccttgacg gagggagagt
      721 gctgagagct cttcttgtgg aacggatggg catgtcgggg gccacccggg cagcggtgaa
      781 cgtttcacgg tggatcggcg gtttggcagt cgtggccggt gcggtgggcg cggcgacagg
      841 agctttgagc ccgctcgtcg cggccatggg tgtgctcatc tgggtggaag cgggacgcga
      901 ggtacgcttc gcaccgtacg cagccatgag atcggcgatg aggaaacggg caggactggc
      961 gcggaatggg ttcgcaccgg cggcgctgat cgccgcaagc ccggacatga gcgcgggcgc
     1021 cgtgctaggc cggctctccg ggcagcaatt cgcgataata tccgtggtgg cgcccgacgg
     1081 cagagagatg ggccgtcttt cggagattga gctgatggat ggggtatgcg cccgggggcc
     1141 tggggtaaac ctggaaacac tggtgagggg aagcaagcat cgatggtgaa caaggaacta
     1201 ctggcaacag agatacttcc gaaggtgtcg cagcccgctc ggtacactgg gcatgagttg
     1261 aatgctggag caggctaccg tgccggcgca gaggccgcac tgcttctggc cttccccgat
     1321 gtgtatgaag tggggatgtc gtatatcggc ttcaagatac tgtacgacat cgtgaacagc
     1381 aacccggtgt gggcggttga acgcgccttt gcgccatggc ccgacatgga ggcacagatg
     1441 cgcctgcacg ggatccctct gtacggcctg gagacttata cgcccgcccg aagcttcgac
     1501 ctcataggtt tcaccctcca gtatgagctg acatactcaa acgttctgtg catgctcgag
     1561 cttgcaggga taccggcgcg tgccgacagg agaggacaag gcgaccccat agtcatcgcg
     1621 ggcgggccat gcgcgtccaa tcctgagccc attgccccct tcctcgactt ggtcgtcgtg
     1681 ggcgatggag aggaggcaat cgtcgagatc atgagcgaga cggctcgagg caagcgcgag
     1741 ggcgcgtcca gggaggcgat cctcgcaagg ttggccaaga ttgccggcgt gtactggccg
     1801 cgagcccaga accatgttac gcggcggatc gtggccgacc tggactcggc gcagtatccc
     1861 aggaagtttg tggtgccgtt tctggaggca gttcatgacc gggtggcgct tgaggtaatg
     1921 cgaggatgct ccagaggatg ccggttctgc caggcaggga tggtttaccg gcccgtgcgc
     1981 gagcgtagca tgaacgacct gtgcgagctg gccgaggcgc ttgtcagcag ttcgggatat
     2041 gaggagctgt cgctactttc actgtccagt gccgactaca cgcgcataaa ggaactcatc
     2101 tccaggcttg cggcagagta cggccctcaa ggcgttgccg tgtcattgcc ttcgctgcgc
     2161 gtggattctt tctccatcgc cctgtcgcgc gatgtagaga agtcgcgccg cacggggctg
     2221 acgttcgctc cagaagccgg gacacagcgc atgcgagaca tcatcaacaa aggcgtgacc
     2281 gaggacgacc tgatctccgc tgccaccgac gcgttcgagt cggggtggga ccagatcaag
     2341 ctgtacttca tgatcggctt gccctatgag accttcgaag atatcgacgg catcgccgcc
     2401 ctgtcgcgac gcgtgctgca attggggcgg caggtagccg gcgcccgtgg caaggccggc
     2461 cgggcgcggg tgaatgtgag tgtgtcgtct ttcgtcccca aaccgcacac tccgttccag
     2521 tggtttgccc agaacacccg cgaacagctg gaacagaagc agtctttcct caaggagcgc
     2581 atgcgggcgc gcggcctttc ggtatccttc cacgacgtgt gggcgagtca cttagaggcc
     2641 gcattcgcca ggggcgaccg ccatctagcg cccgtcattg agcgcgcagt ggccttgggc
     2701 tgcaggtttg acggttggtc ggatcagttt cggccggatc tatggcagcg ggcctttacc
     2761 gacatgggcc tggattcctc catgtgggcc aatgccgagt acgggttcga tgattcgctg
     2821 ccttgggacc atatcgacat gggtgtctcc cgcgagtttc tggtgcgcga ggcgcgccgc
     2881 gcagagagcg ggctcaccac gcctgactgc cgcgaggccg gctgttccgg ctgcggcgtc
     2941 tgccggggcg atgtccgtgt caggctggct ggggcatttg cgggcagctc ctcgcaaggg
     3001 ggcggacagg catgagcgcg gcagtagtgc gggtgcggct cggaaagggc gacgagataa
     3061 ggtttgtgtc gcacctgggt ttcatgagag cgtttgaacg tacagtgagg cgtgctcgga
     3121 ttccggtggc ctactcgggc gggtaccatc ctcaccccaa gatctcgttt gcgactgctc
     3181 tgggtgtggg tatgaccagt gaggctgaat acctcgatat ggagctcgcc ccggccgact
     3241 cgtgcgacct gtctacacag gatgtgtttg agaggctgca accgacggcg ccgccgggga
     3301 tgccggtgat agcggtgtgc gctgcagagg gcgggccggc tcttgcctct cgcgtagaat
     3361 gggcatccta ccgcgtagac gttcatgctg atgcacggcc tgaactggag gacgcggtac
     3421 tagagtttct ttcgtccgac agcgcgattg ccaaggtcaa cactaagagc gggcagcgca
     3481 ggaccgacat acggcccatg gtgcgcgaaa tccgctggtc aggcagtggc gctctagagt
     3541 gcacgctggc atgcggagga cgggcccacc tccgcccgca ggacttcatt gcccatcttg
     3601 actcgatgcg cggccgcggt gacgcgccta tgttggggca tgagtccatc ggtccatccc
     3661 gtatccatag gacgggcctg tatttcactg atagagaagg cggggatttg ttgtctccga
     3721 tgaatgttga atgcaaagat ttggttatgg cgtggagttg acccgaggga gttgagttaa
     3781 gtggccagag agattatcgt caactccgaa agcggcgaag ttagagccgc agtcctcgaa
     3841 gatggagtac tggtcgactt gtttatcgaa cggtctcttc atccgaggta cgccgggaac
     3901 atatacaagg gaaccgtcga gaacgtgctc cccggtatgc aagcggcctt tgtcgatgtg
     3961 gggttggaac gaaacgtatt tctgtatgta gacgatgccc ttgcggggcg aaacggccgt
     4021 gaagcgcgga ttgtgcgcga agcggacggc gaagagatca ccgtaccgaa caagaagcag
     4081 cgctcgataa aggacattct caaacccggg caggagatta tcgttcaggt tagcaaggaa
     4141 cctatcggaa ccaagggcgc ccgggtggtg accgatatca cgcttccagg caggtatgtc
     4201 gtgctgatgc cgaccgtgga ctacgtgggg gtttcgcgga ggatagacga agacggtgag
     4261 cgggacagac taaagaaaat cgcacaggca gctaagccca agcggatggg cgtcatcgtg
     4321 cgcacggttg ccgaggggtt gagccaggag gagatcgcct ccgacattca gttcctggtg
     4381 aaactgtggc gcaggattca ggcccgaggc agacgagcca agccaccggc attgctgcac
     4441 aaggattacg atctagtgtt ccgactggtg cgcgaccatt tcgcagaaga tgtcacgcag
     4501 tttgtcgtgg acgacccgga cgacctgaag aaggtcaatg atcttgtaaa gatgttcccc
     4561 gaacccatgc gcgacagagt gcacctctac acgggtcagg agccgatctt cgattcgtac
     4621 ggtctggaag aggagatagc acgggcgctg agacgcaagg tatggctggc ctgcggcggc
     4681 tatcttgtga tcgaccacac tgaagccttg acggtgatcg atgtgaacac ggggaagttc
     4741 actggatcca catgccttgc cgacaccgtc ctgaagacca atctggaggc tgcctcggaa
     4801 attgcgcgtc agatgagatt gcgaaacatc ggcggcataa tcatcgcgga cttcatcgac
     4861 atggacgatg agaaccacca gaagcaagtc gtgcgcaaac tcgaggaatc gctggcgcgc
     4921 gataagacca aagcttccgt gttgggcttc actcagcttg gcctggtaga gatgactcgc
     4981 aagaaagtgc agcagggtct ggcagagtct atgatgaaag tgtgccccat gtgcgacggc
     5041 cggggaagga tcctctccga ggagactctg gcgttccgtg cgatgcgcgc tatcaagaag
     5101 gaagctctat cgacatctca gccggcgatg ctggtgttgc tgcacccatc ggtggccgca
     5161 gtgctcatag ggtcgggtgg atccaacctt gcggctctgg aagaggagac cggcagggcg
     5221 atatacgtca aggggagttt cgaccagaag gtcgagg
//
LOCUS       DAOTAC010000183         5203 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_13323_length_5203_cov_4.393162, whole genome shotgun sequence.
ACCESSION   DAOTAC010000183 DAOTAC010000000
VERSION     DAOTAC010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5203
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13323_length_5203_cov_4.393162"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="PLM74_08245"
     CDS             complement(<1..248)
                     /locus_tag="PLM74_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HON43101.1"
                     /translation="MSLRRHIEIVVQDALISCNELPDSAIRIRMLLHRRGPILVSEII
                     EELKMSPNTVRSSCRKLISKGWAAKMNLPDGRRRLIIP"
     gene            627..1181
                     /locus_tag="PLM74_08250"
     CDS             627..1181
                     /locus_tag="PLM74_08250"
                     /inference="COORDINATES: protein motif:HMM:NF021348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HON43102.1"
                     /translation="MAPPKYTARDLVFIALMAAAGIAIKPLIVPVIRVVAVPLGLPGG
                     SLAGGIYMMWLILGHAFTGKPWAATLVGVVQAILAWAMGIPGPQGPWMLVMYMAPGMA
                     ADLVLRFSSGSAAALLLGAAMLAGAAANVTGISLMCLIIYRISLPVLVLSMAVGAVSG
                     AIGGVVAYAIHRRVAQIAKQTHGG"
     gene            1186..1929
                     /gene="mobB"
                     /locus_tag="PLM74_08255"
     CDS             1186..1929
                     /gene="mobB"
                     /locus_tag="PLM74_08255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HON43103.1"
                     /translation="MRVFAVSGVHHSGKTTTIEHLIRELVRRGYSVGTVKSVHREKFS
                     IDRAGTNTDRHRSAGATAVTAVSDVQTAIMWSGRLDYDAILRFYDTDWVIIEGAHREP
                     YPRIVAAGTEHHIEQRFDANTFLICGAVAQKLDSWHGVPVIDATTRAKEVVDLLERSV
                     PEFVRPEGGHSERGHSERGHSELDRPEQYPPEPGLEERFEAEVRVDGEELHLAPFVQD
                     FVSRTVLGMLSSLKGYKPGSEFKIEIRRF"
     gene            1934..2962
                     /locus_tag="PLM74_08260"
     CDS             1934..2962
                     /locus_tag="PLM74_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43104.1"
                     /translation="MLPQDLRVLPELSPAPPAEATQPLLAAAIKSARIVSALADRKSD
                     TLLLRVDAGASAGASASASAGAGVGVGAEASAGGHDLLLVLKVYDEAHPGRGASTGRA
                     GREADALLRLAQAGVCVPGLVASGKRALLRRYVEGPIVRDLHWDVTLARQLAEWVFRC
                     HEALAIDAIRGTDSVGVIDAIGGMDSAGADESSCVADMTGVADMTGAAGTTGAAGHGL
                     RRSWLVGDMNLGNFVLDVASGRLCGIDLGDTRVGDPLDDVGEGCMRIVGRRPGFTADR
                     WECAVAFASWYGRLAGSVEYVLSRIPERASASFRQMAVWRDDPFMAEVADAFQILWGD
                     ALLSLNNG"
     gene            3016..3477
                     /locus_tag="PLM74_08265"
     CDS             3016..3477
                     /locus_tag="PLM74_08265"
                     /inference="COORDINATES: protein motif:HMM:NF025070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HON43105.1"
                     /translation="MKAEFVNPFASAVLMVFQKEFGMNVMRDSLALQQGPLRGADVNT
                     IIGVTGQLEGQVIYTFEEGVALRIASALMGEAVEELDELAKSAVAELGNIITGNAAIG
                     LSENGYNCILTPPTLLTGKELIVTTFTPVLNIPFSTDFGSVIVHVALRERP"
     gene            3878..4621
                     /locus_tag="PLM74_08270"
     CDS             3878..4621
                     /locus_tag="PLM74_08270"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body protein"
                     /protein_id="HON43106.1"
                     /translation="MIRGIYIAASGMMAQEAKQSAISNNLANVSTPGFKRDIAVGESF
                     SDIVSGLARSPIGRLGMSTAITAVCFDNSPGSMASTENPFDLAIEGDGMFVVQTDAGI
                     RYTRTGNFRLDAESFLITQDGHRVLGVDGPIRVDGSLVVQNDGSVYSDGEFRGRLSVM
                     VPTTGDGLVKVGGGLFAERAGAAAGMVQVQPGEARVIQGQLESTNVNVVREMVDMIAG
                     YRTYEAAQRALLSHDALLDKAVNEVGRVG"
     gene            4768..>5203
                     /locus_tag="PLM74_08275"
     CDS             4768..>5203
                     /locus_tag="PLM74_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HON43107.1"
                     /translation="MMRALWSAATGMLAKQLDMDVIANNLANVNSAGFKKSRVDFQDL
                     MYQTIRTAGSTVAQGSKVPTGIQVGLGTRAAAIQKVFSQGDFIQTENPLDLVIEGDGF
                     FQVLLPDGSTAYTRTGAFKVDSEGRVVTSDGFVMEPELVVPAN"
BASE COUNT         1066 a         1355 c         1716 g         1066 t
ORIGIN      
        1 atggggatta tcagcctgcg ccgtccatca ggcaggttca tctttgccgc ccaaccctta
       61 ctgatcagtt tccgacacga actccggact gtgttgggtg acatcttgag ctcttctatt
      121 atctcgctga ccagtatcgg acctcgccta tgcagcagca tccgaattcg gattgcacta
      181 tcaggaagtt cattgcatga tataagagcg tcctgcacga ctatctcgat gtggcgccta
      241 agagacatgg acatcccctc cgtcaagttc cagttatgga tgcatgagcc tttagctatc
      301 attataccac gaactgtaac tagacccaaa acgtagccta tcctctcgcc ggctggccag
      361 aaacgagcgc cgcggccgga aaggagccat ctgttgggct cctcggctgc ggcgcccacc
      421 gagcaccgga tcgaagcatc ttgcatgccg agctcgatac gccgcctctg cctgcagcgt
      481 tgttctctac tcccccggca acaggggagg aaggggaggg aaagcaggag ttcatctaga
      541 atacacgaca aactgcatca actacatctt ggctgcttgc gcactgtcga tgtgtggcat
      601 aggcccgggc taggagggat tcccgcatgg cgccgcccaa atacacagcc cgtgacttgg
      661 tgtttatcgc tctgatggcg gcggcgggga ttgcgataaa gccgttgatc gtgccggtga
      721 tacgggtcgt tgcggtcccc ctggggttgc ccggcggctc attggccggc gggatctaca
      781 tgatgtggct gattctcggg catgcattta ctggcaaacc gtgggcggcc accttggtag
      841 gggtagtaca ggcgattctg gcgtgggcga tgggcatccc agggccccag ggcccatgga
      901 tgttggtaat gtacatggcg ccgggaatgg cggccgatct ggtgctgcgt ttcagctcgg
      961 gcagcgcggc agcattgttg ctcggggcgg cgatgctagc cggagctgcg gccaatgtca
     1021 ccgggatatc cctgatgtgc ctcatcatct atcggatctc tcttccggtt ctggtgctgt
     1081 ctatggccgt gggggcagtg tcgggagcca ttggcggtgt tgtcgcatac gccatacacc
     1141 gtcgggtagc gcagattgct aagcagacgc atggaggctg actgcatgag agtattcgcg
     1201 gtctcgggag tgcatcactc cggcaagacg actaccatag agcatttgat cagggagctc
     1261 gtcaggcggg ggtatagtgt tggcacagtc aagagtgtgc accgggagaa attctccatt
     1321 gaccgagctg gaaccaatac agacaggcac cgttcggcgg gcgccactgc tgttacggct
     1381 gtgagcgatg tacagacagc cattatgtgg tctggccgac ttgactacga tgcgattttg
     1441 cgtttctacg atactgactg ggtcattatt gagggcgccc accgggaacc gtatccgcgt
     1501 atagttgcag ccggcacaga acaccatatc gagcagcgct tcgatgcgaa cacgtttctc
     1561 atatgcggag cggtggcgca gaagcttgat tcatggcacg gagtgcccgt gatagatgct
     1621 accacacggg caaaggaggt agttgatctg ttggagcgca gcgtcccgga gtttgtacga
     1681 ccggagggcg gtcactcaga gcgtggccac tcagagcgtg gccactcaga gctggatcgc
     1741 ccggagcagt atcccccaga acctggcctt gaagagcggt tcgaggccga agtgcgcgtt
     1801 gacggcgagg aactacacct ggccccattt gtgcaggact ttgtgtctcg aacggtgctc
     1861 ggcatgcttt cttctctgaa gggttacaag cctggaagcg agttcaagat tgagatcagg
     1921 cggttctagg cgcatgttgc cgcaagacct acgggttctt ccggagctga gtccggcgcc
     1981 gcccgcagag gcaacacagc ccctgctggc agcggcgatc aagtccgcca ggatagtttc
     2041 tgcgctagcc gaccgcaaga gcgacactct cctgctgagg gtggacgcgg gtgcgagcgc
     2101 tggtgcgagc gcgagcgcga gcgcgggagc tggggtagga gtgggcgctg aagcgagtgc
     2161 aggaggccat gacctgcttt tggtcctcaa ggtgtacgac gaggcgcacc ctggccgagg
     2221 tgcaagtacg ggccgcgcgg gcagggaggc agacgcgctc ttgcgtctgg cacaggcggg
     2281 ggtttgtgtt cccgggctgg ttgcgtcagg gaagcgtgcg ctgttgcgcc gctacgttga
     2341 gggaccgata gtaagggacc tgcattggga tgtgactctc gcccgtcagt tggcggagtg
     2401 ggttttccgg tgccatgagg cccttgccat cgatgcgatc cgagggacgg actcggtcgg
     2461 cgtgatcgat gccatcggcg ggatggactc ggccggtgct gatgagtcgt cttgtgtcgc
     2521 ggacatgact ggcgtcgcgg acatgactgg cgcggccggc acaactggtg ccgccgggca
     2581 cggcctcaga cgctcgtggc tggtgggcga tatgaacttg ggcaatttcg tgctcgatgt
     2641 tgcgtccgga aggctgtgcg ggattgacct tggtgacacc cgagtgggcg acccgctgga
     2701 cgatgttggt gagggatgca tgcggatcgt tggccgcagg ccggggttca cagccgacag
     2761 gtgggagtgc gctgtggcgt ttgcctcttg gtacggcagg cttgccggaa gtgtcgaata
     2821 cgtcctctca aggattcccg agcgcgcgtc tgcgtcattt cggcagatgg ccgtgtggcg
     2881 agatgacccg ttcatggcgg aggtagcgga tgcgtttcag attctctggg gagatgcgtt
     2941 actttcgctg aacaacggct aaacaagagg agccgttcag ccgatagata atgcggaggg
     3001 aacggagggc gaggtatgaa ggcggaattt gtgaacccct ttgcgtctgc agtgctgatg
     3061 gtgttccaga aagaattcgg gatgaatgtc atgcgtgact cgcttgcact tcagcagggg
     3121 ccgcttaggg gcgcggacgt aaacacgatc atcggtgtta ccgggcaact tgaaggtcag
     3181 gtcatataca ccttcgaaga gggagtagcc ttgcgcatcg catccgcgct gatgggtgag
     3241 gcagtggagg agctagacga actggcgaag agcgcagtgg ccgaactggg gaatatcatc
     3301 accgggaatg ctgctatcgg cctgtcggag aatggttaca actgcatact gactccgcca
     3361 acgctgctca caggcaaaga actcatagta acaactttta cgccggttct taatatccca
     3421 ttctccaccg acttcggaag cgtgatagtc catgtggctc tgagggagag accctaggct
     3481 ggatcactga gaaaatcttg cccggttcgg gaaacaaatg cacttatcga cgaaagacgc
     3541 tccagggagg gggcgtcttt tgtttgccct gacttcatat cgaccgatat caagagtgcg
     3601 acgcatgagc atccggcgca gctacagacg ggtatgatgg tcagagaccg tcgacggggc
     3661 gccttggcat gtatcttgct tcaaacaaat ggcgggcgca tgtttctcgc aactggcaac
     3721 tcgcaacgtg ctgcccgtca cttatcactc tccactcgcg ctgcaccagt tacagttgca
     3781 gcggcagcgg cagcggcagt tgtggtggca gcggcagttg cagttgcagt tgcagtggcg
     3841 gtcgagggaa tccacatgag agaagggagg gtaggtattg atacgaggga tctacattgc
     3901 cgcatcgggc atgatggcgc aagaagcgaa gcaatcggcg atctcaaaca acctcgccaa
     3961 cgtatcgact ccagggttca aacgcgatat cgcagttgga gagtcattct ccgacatcgt
     4021 ttccggcctt gccaggtcgc caattggccg cctgggcatg tcgactgcaa tcactgcagt
     4081 ctgttttgac aactccccag ggtcgatggc gagcacagag aacccgtttg acctagcgat
     4141 agagggcgac gggatgtttg tggttcagac ggacgcaggc atcagataca cgcgcaccgg
     4201 caacttcagg ctggatgcag aatcattcct aatcacgcag gatgggcaca gggtgctggg
     4261 cgttgatggc ccaattaggg tagatggatc tttggtagtg cagaatgatg gcagcgtcta
     4321 ttcggacggg gagtttcgcg ggcggctgag cgtcatggtc ccgacgaccg gcgacggact
     4381 ggtcaaggtg gggggagggc ttttcgcgga acgggctggg gcagcggcag gcatggttca
     4441 ggtgcaacct ggcgaggcca gggtaatcca ggggcaactg gagtcgacga acgtgaatgt
     4501 agtgcgcgaa atggtggaca tgattgcggg ctacaggacg tacgaagcgg cgcagcgagc
     4561 tctgctttcg cacgatgcat tgctggacaa ggcggtcaac gaggtaggta gagtagggta
     4621 gggctggaga cggtaggaag ggtagatcag gacgacacgg gacaacacgg ggcgacacaa
     4681 tttcacatgc ggccggaccg cgcacgcggg ggccgcgggc tgccgactga ttgacgcagc
     4741 ccacacaata aggaggtctg taccgccatg atgagggcgt tatggagcgc ggccaccgga
     4801 atgctcgcca agcaactcga tatggatgtc atcgcgaata acctcgccaa cgtgaacagc
     4861 gcgggtttca agaagagcag ggtagatttc caggacctca tgtaccagac catcagaacc
     4921 gcaggcagca ccgtcgccca gggatccaag gtgcccaccg gcatccaggt gggtctgggg
     4981 accagggctg ccgcaattca gaaagtcttc tcccagggcg acttcattca aacagagaac
     5041 cccctcgatc tcgttattga gggagacggt ttcttccagg tgctcttgcc cgacggttcg
     5101 acagcataca ctcgcacggg cgcattcaag gtcgattctg aggggcgagt ggtgacctcg
     5161 gacggattcg tcatggagcc tgaactagtt gtgccggcca acg
//
LOCUS       DAOTAC010000184         5155 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_13458_length_5155_cov_3.952549, whole genome shotgun sequence.
ACCESSION   DAOTAC010000184 DAOTAC010000000
VERSION     DAOTAC010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5155
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13458_length_5155_cov_3.952549"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1215)
                     /locus_tag="PLM74_08280"
     CDS             complement(<1..1215)
                     /locus_tag="PLM74_08280"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HON43108.1"
                     /translation="MITEQRKSWHAMSSGEALVALGTSRASGLSNEDAQARLSEHGPN
                     SLAEPARRTLLEQFVAQFKEFLVVLLLISAGVSAAVGEVEDALVIGVIVVLNAVIGVA
                     QERRAENALDALKKMASPRARVIRSGEPRLVEASSLVPGDIVLIEAGDSVPADARLVE
                     SAMLKLDESSLTGESVPVDQNAEVILDDDAPLGDRVNMVHMGSSVTYGRGVAVVVATG
                     MSTQMGRIAAMLQEGEQETTPLQQKLEVLGKKLGTAAVLMCAGVFIAGLARGERAFDM
                     FLTAVSLAVAAIPEGLPAIVTIVMAIGVQRMATQNAIIRHLPAVETLGSADVICSDKT
                     GTLTTNQMTVRRLYVPGRAVDVIGEGYSRMGALLEAGSDALVSPDASVSRMLTCAILC
                     SDARVRDVGDGGR"
     gene            complement(1476..2069)
                     /locus_tag="PLM74_08285"
     CDS             complement(1476..2069)
                     /locus_tag="PLM74_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HON43109.1"
                     /translation="MRHYFERARAPAVYEGAAQQHVHDLKYRARVEIVPALSAIMATC
                     AIHTGMANTCDCAAPVPLHPDRLARRGFNQAELLARSVASLLGITLIPEALSRRASTG
                     TQTFLDRRHRRDNVLRAFVCDEPARVAGRTVMLIDDVITSGATADGCARALLRSGASR
                     VYVLTFAVSVADARDWAPSRNPGSAVHTGQRRMEGRV"
     gene            complement(2327..4120)
                     /locus_tag="PLM74_08290"
     CDS             complement(2327..4120)
                     /locus_tag="PLM74_08290"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HON43110.1"
                     /translation="MFHAYILLTESGARAGFGHSPGFDWAYANTRAPTDLAVITPPVP
                     AAYAEVVSGEVRRQLDGVSRAHPGRARHAAVRAGLRTARSVLRAFGLPGPAFLSAGSF
                     WHSSRFDAERVRDRIPPPSLVTESAIALAGRSLLYEEAMRALASGGFSSLDGAHDLLA
                     AICADGLGLLLPAVSISPLGARICNRCGSRGPFASTPCAHCGRQACFLCLQCRSMGPA
                     RECVPLYLVADHECASFRAGAVAEGAHLAMPHALTDAQEQAAGMLRDWYADGSTRDVL
                     VWAVCGAGKTEVSFSAIHSALMAGQRVLFAAPRRDVVAELAPRVQAAFPGVTCSTFCG
                     GSIGQRDYDARIILATTHQTMRFYRRFGLVVLDEADAYPFAGSEALASSVRRAIAPEG
                     RLVIMTATPSRGHLARVNSGVLPCIRIPARHHRKPLPEPEVVTAPGSDLLPKVADIAC
                     ASVRSGSPLLVFVPRRRQAQEVAGYLSGKVPGARVEWVHSQDPARDHKRAAIAGGKCD
                     VLVSTSVMERGVTVDGVDVVVCYADDDIVFDYRALIQMAGRSGRTAANPGGRVWFVCA
                     NENWHARMAAGIIKEMNAEATARGLLAGSHG"
     gene            complement(4224..>5155)
                     /locus_tag="PLM74_08295"
     CDS             complement(4224..>5155)
                     /locus_tag="PLM74_08295"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HON43111.1"
                     /translation="VDISLMYSVLKAVRPGSRLVLVGDVDQLPSVGPGNVLRDIIDSG
                     VVPTVVLNQVYRQAPGSMIVTNAHRINHGEFPLFRGARDFFFIEAEDPDRVVQAVVTA
                     VTSKLPRHLGCDPVDDVQVLSPMKRSAAGVDNLNVVLRDALNPRRAGASEIRCRGSAF
                     RRGDKVMQIRNDYDRLVWNGDMGKIVRIDAEEGEVVVRFSEPEGDRDVLYHQDELDEL
                     TLSYCVSIHKSQGSEYPAVVVPVTTQHYIMLQRNLLYTAVTRARRTVVLIGTKKALAI
                     AVRNASMLQRRTMLAPRLREATSASWPEEEQIG"
BASE COUNT         1055 a         1694 c         1470 g          936 t
ORIGIN      
        1 ccggccgccg tcgcccacgt cccttactct ggcatcgctg cacaaaatgg cgcaagtgag
       61 catacgagac acagatgcat caggcgaaac aagcgcgtcg gatcctgctt cgagcagagc
      121 ccccatacgt gagtaaccct caccgatcac gtcgacggct cgccccggaa cgtacagcct
      181 ccgcacggtc atctggttgg tggtcagggt gcctgtcttg tcggagcata tgacatccgc
      241 gctccccagt gtctccactg caggcaagtg gcgtatgatc gcgttttgtg tggccatccg
      301 ctgtacacct atggccatga ctatggtgac aatcgccgga agcccctcgg gaatcgcggc
      361 tactgcaagg ctcactgcag tgaggaacat gtcgaaagcg cgctccccgc gggcaagccc
      421 cgcgatgaac accccggcgc acatgagcac cgccgctgtt cccagcttct tgccgagtac
      481 ctccagcttc tgctggaggg gtgtggtctc ctgctcgccc tcttggagca tggcggcgat
      541 tcgacccatc tgggtggaca tcccggtagc caccacgacg gccaccccgc ggccgtaggt
      601 cacggaactg cccatgtgaa ccatgttcac cctatcgcca aggggcgcat catcgtccag
      661 gatgacctcg gcgttctgat cgaccggaac cgactccccc gtgagtgagg actcatccag
      721 cttgagcatg gccgattcga ccagcctggc gtcggcaggt acgctatcgc ccgcctctat
      781 caagacgata tcgcccggga cgaggctcga agcttcaacc aacctgggtt cgcccgacct
      841 gatgacgcga gcgcgggggg acgccatctt cttcagggca tccagggcat tctcggccct
      901 gcgttcttga gccacgccga tcacggcgtt caggacgact atcaccccta tgaccagcgc
      961 atcttctact tctcctacag cagccgatac gccggccgat atgaggagca ataccacgag
     1021 aaactcctta aactgcgcga cgaattgctc cagcaacgtg cggcgcgcgg gttcggcaag
     1081 gctgttcggc ccatgctcgc tgagacgcgc ctgagcgtct tcgttcgaca aacccgacgc
     1141 gcgcgatgtg cccagtgcga ccagcgcctc gccagacgac atcgcatgcc atgacttcct
     1201 ctgttctgtg atcaagattc gagcctccaa gttctccgag atcttctacg ccatcaagct
     1261 catgaagact gcaatgccca gcacagcggc ggacgggaca agcaccccgc ttagcatccc
     1321 ccggaaggcc cacatacgcc atggccataa ccatgcttag tgcgggcgcc gaggaaacag
     1381 acctgataac agatggtcca gttagcaccg attatatatc cgaccaacta tgcacgcaac
     1441 gcaccaaagg ctcctgttgc taccgcctcc agggatcaca cgcgcccctc cattctgcgc
     1501 tgcccggtgt gcactgcgga gcccgggttg cggctaggcg cccagtcgcg cgcatccgcc
     1561 acggacacgg caaatgtgag tacgtagact cgggaggcgc ccgaccggag caaggctctt
     1621 gcgcacccat cggctgtggc gccggatgtg atcacatcat cgatgagcat gacagtacgg
     1681 cctgccacgc gggcaggctc atcgcagacg aacgcgcgga ggacgttatc acgtctatgc
     1741 ctccggtcaa gaaaggtctg ggttccagtg gacgcacgtc ttgagagcgc ttcggggatc
     1801 aatgtgatcc ctagcagcga tgccacagat cgcgccagca gttcggcctg gttgaatccg
     1861 cgcctcgcca acctatccgg gtggagcgga accggtgccg cgcagtcgca cgtgttcgcc
     1921 atgcccgtgt ggatagcgca tgtcgccatg atcgcagaca aggcggggac gatctccaca
     1981 cgggcacggt atttcagatc atgcacgtgc tgctgcgctg cgccctcgta aaccgcaggc
     2041 gccctggcgc gttcgaaata gtgccgcaag ccgcggcatc ccatgcacac ctcatgttca
     2101 gcgtcgtcgt gcctctcaac gccgccccgt agaggcgtcc cgcagacgct gcatataggc
     2161 gggcgggcca tcggaatcaa cgccatacat tcgctacaca acgagtgcgc gttcccaggc
     2221 ctaccacacg ccgggcagaa ctccctcgtc ccgaacaaga catccaatgc aatgccgaga
     2281 acccgtgcaa tgctcattgc ggtatcacct ccccacatga tgtcagctag ccgtggctac
     2341 cagccagaag cccacgtgcc gtcgcctctg cgttcatctc cttgatgatt cccgcagcca
     2401 ttcgagcatg ccagttctca ttcgcacaca cgaaccagac tcggcctcca gggttagccg
     2461 ccgttcggcc acttctccct gccatctgga tcagagcccg gtagtcgaac acaatgtcgt
     2521 catctgcgta gcacacaacg acgtcgactc cgtctactgt caccccacgt tccataacgg
     2581 acgtgctcac gagcacatca cacttacccc ccgctatcgc cgcacgctta tggtcccggg
     2641 ccgggtcctg cgagtggacc cactccaccc gggctccggg gaccttgccc gacagatatc
     2701 cggccacctc ctgagcttgc cggcgtcggg gtacaaacac cagaagcggc gatcccgacc
     2761 tgacgctggc gcacgcgatg tctgccactt tgggcagcag gtcactgcct ggcgcggtca
     2821 ctacctccgg ctctgggagc ggctttcggt ggtgacgcgc aggaatcctg atgcatggca
     2881 gaacccctga gttcacccgc gcaaggtggc cacgcgacgg tgtcgcagtc atgatgacaa
     2941 gccgcccttc gggagcaata gctcttcgaa ccgacgacgc aagggcctcg gagcctgcga
     3001 acgggtaagc atcggcctca tcaagaacga caagaccaaa tctgcgatag aagcgcattg
     3061 tctgatgggt tgtggcgagt atgatgcggg cgtcatagtc cctctgaccg atggaaccac
     3121 cacagaacgt ggagcacgta acgcctggaa aagcagcttg tactctcggt gccagctcgg
     3181 cgactacgtc gcgcctgggt gctgcaaaaa gcaccctctg gcccgccatg agcgcagagt
     3241 gtatggcgct gaacgatacc tcggtcttgc ctgcgccgca caccgcccac accagtacgt
     3301 cgcgagttga accgtcggcg taccagtcgc gcagcatacc ggcagcctgc tcttgagcat
     3361 cggtaagtgc gtggggcatc gcaaggtgtg cgccttccgc gactgccccg gcacggaaac
     3421 tcgcgcattc gtggtcggca accaggtaca gcgggacaca ttcgcgcgcc ggccccatcg
     3481 acctgcactg cagacagagg aaacaggcct gcctgccgca atgggcacat ggtgtagatg
     3541 caaacggacc acgagagccg catctattgc aaattcgcgc ccccagaggc gagatggaga
     3601 ccgcaggcaa gagcaagccc aggccgtcgg cacatatggc tgccagtaag tcgtgagccc
     3661 catcgagaga cgagaatccg cccgaagcga gggcacgcat cgcctcctca tacagcaagc
     3721 tgcgaccggc caacgcaata gcgctttcgg tcacgagact tgggggagga atgcggtctc
     3781 tgacccgttc cgcatcgaaa cgcgatgagt gccagaatga cccggcagag aggaatgcag
     3841 gaccaggtaa cccaaaggcg cgcaagacag accgagccgt cctcaagccg gctctcaccg
     3901 ccgcgtgtcg agccctgccc ggatgtgcgc gtgatacgcc gtccagctgc cgccggacct
     3961 cgccagacac tacttctgca taagccgccg gaacaggggg agtgattacg gccaggtctg
     4021 tgggtgcccg ggtgttggcg taggcccaat cgaagccggg cgaatgaccg aacccggcgc
     4081 gcgcgccgga ctccgtgagt aggatgtagg catgaaacac tcgtcaaccc tgcctattcc
     4141 agtttatgtt ggtacattac attaggcagc ggctggaagg ttccttccga aagaatcaaa
     4201 atcgcccggg ttagcccggg catctatcct atctgctcct cttccggcca ggatgccgag
     4261 gttgcttcgc gaagcctcgg tgcaagcatt gttcgtcttt gcagcatgga tgcattgcgc
     4321 acagctatcg ccaacgcctt tttggtgcct atgagcacaa ccgtccttcg agcgcgggtc
     4381 acggccgtgt agagcaggtt tcgctggagc attatgtagt gctgggtagt aacgggcacc
     4441 acgacagccg ggtactcgct cccctggctc ttgtgtatcg acacgcagta cgacaatgtc
     4501 agttcatcca gttcatcctg atggtacagg acatcccggt ctccctccgg ttcagagaac
     4561 ctgacgacta cctcgccttc ttcagcatct atgcgcacga tcttccccat gtcgccattc
     4621 cataccagcc ggtcgtagtc gttccggatc tgcatcacct tgtcgcctct gcggaacgcc
     4681 gagcctctgc agcgtatctc agaagctccc gctcgacgcg gattgagcgc atcacggaga
     4741 accacattga ggttgtcgac gcccgcagcc gaccgcttca tcggtgacag cacctggaca
     4801 tcatcgacag gatcgcagcc caagtggcgc ggcagtttcg aagtgacggc agtcactact
     4861 gcctggacca cccggtcagg atcctcagct tctatgaaga agaagtcgcg cgcgcctcgg
     4921 aataggggaa actcgccatg gttgatccgg tgggcgttag tgactatcat actgcccggc
     4981 gcctgcctgt agacctgatt gagcacgacc gtcggcacga cgccggagtc tatgatgtcg
     5041 cgaagaacat tgccgggtcc gaccgatgga agctggtcga catcgcccac gagaaccagc
     5101 cgcgatccgg ggcgcactgc cttgagaacg ctgtacatga gcgagatgtc gacca
//
LOCUS       DAOTAC010000185         5140 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_13508_length_5140_cov_5.256244, whole genome shotgun sequence.
ACCESSION   DAOTAC010000185 DAOTAC010000000
VERSION     DAOTAC010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5140
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13508_length_5140_cov_5.256244"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="PLM74_08300"
     CDS             complement(<1..382)
                     /locus_tag="PLM74_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43112.1"
                     /translation="MEIGGEFCFADIGRAPDGEASVADLIDGWSRGYPTLLMSSGRTA
                     YLNVWLNLLHAEHGLDTVLLPSYLCYSILMPFRRMGVRIEYYRVDADLAVDWEDLERK
                     AGRLGARAAAVIIDYFGLIPSGDSA"
     gene            complement(402..1151)
                     /locus_tag="PLM74_08305"
     CDS             complement(402..1151)
                     /locus_tag="PLM74_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON43113.1"
                     /translation="MLLGIENLHVHYGAIHALKGISFNVDRGEIVTLIGANGAGKSTV
                     LRTISGLIRPTDGSMSYWVPPATSKADAAGRQVNLCSLPAHKLVDLGISQVPEGRRIF
                     PNLTVMDNLELGAYLRHDKAEIKRDFEMVFELFPRLRERRAQAAGTLSGGEQQMLAMG
                     RGLMSRPDLLLLDEPSMGLAPLLVKEIFQVIVRINQSGTAILLVEQNASMALAVANRA
                     YVLETGSIALQGSAAELAKDSRVQAAYLGSA"
     gene            complement(1151..2959)
                     /locus_tag="PLM74_08310"
     CDS             complement(1151..2959)
                     /locus_tag="PLM74_08310"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HON43114.1"
                     /translation="MAEADTASAKRTKTNAKATDGRRLARAAVTLGGLAAGYFLVVAL
                     HRADILINDYYLQILIWVGINVIMGVSLNLINGFTGQFSLGHAGFMAIGAYVAASMTK
                     LMGMPFALALLAGGIAAAAGGLVVGIPTLRLAGDYLAIATLGFGEIIRVIILNLDVVG
                     GPRGLPGIPAHTTFLWVYVIAAATVVVVRNLIWSRQGRALVAIREDETAAETMGVDTT
                     RNKIMAFVVGAFFAGVAGGLYAHKITFIDPSQFDFMKSIEALVIIVLGGLGSITGTVM
                     AAFVVTFLPEILRAFADYRMIIYSLMLILMMLYRPRGLMGTAEFSFKQLGLGNGRPVP
                     DGGGQVAATGDGPPPVLETHRVCMDFGGLRAVSDFNMTLCQGELVGLIGPNGAGKTTV
                     FNMITGLAEATSGRIHFMGEDITRRKPSEINDMGIARTFQNIRLFSNLSVLDNVRIAF
                     RGARPYSMTEALLRAGRYSAQEDQIYRKSMEFLRLFGLEDRASEIASKLPYGAQRRLE
                     MARALATKPRLLLLDEPAAGMNPQETQELLELIRWIHERFDLTVLLIEHDMNLVMKLC
                     QRIIVLDYGVTIADGSPAEIVKNRRVIEAYLGEEAV"
     gene            complement(2970..3842)
                     /locus_tag="PLM74_08315"
     CDS             complement(2970..3842)
                     /locus_tag="PLM74_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HON43115.1"
                     /translation="MVIQQLVNGLALGSVYALIALGYTMVYGIIRLINFAHGDIFMLG
                     AYYGYFAITMFNIPFALALPLTMILAAVTGMAVERLAYKPLRQAPRIASLITAIGVSL
                     LLQNLGLKFFEARPRPYPRIMSMVVWDVGGVVVSNVQLIMLGLAIGLMVLISLIVYRT
                     KIGKAMRAVSFDKDAARLMGIDADMVISVTFALGSALAAAGGMLVGFYYNRISPTMGT
                     MYGLKAFVAAVVGGIGIVPGAMLGGLIMGVAEVFVIAFVSSTLKDAIAFAILIVILLF
                     KPAGLLGRAVREKV"
     gene            complement(3984..5120)
                     /locus_tag="PLM74_08320"
     CDS             complement(3984..5120)
                     /locus_tag="PLM74_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013386872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HON43116.1"
                     /translation="MRKSLVLVVSVLLIMSAVALGAEEIVIGLVTPVTGDVATFGMSV
                     RNAALLWEKQVNSRGGIQGRKVKIIVEDDKGVPSETANAVSKLINQSKVLMIMGPVTS
                     KCANAGAPIGQASKVPVLTPTATDQAVTAVGNYVFRACFIDPFQGQVMGKFALETLKA
                     KTAAMLYDIGNDYTKGLAENFKKFFEAGGGKVVVSETYGVGESDFSAQLTKIKRADPD
                     VLWLPDYYNTVGLITRQARQLGIKRTVFLGADGWDSSELIPIAGDSIEGGFFSNHYYP
                     GSTDPVAVAFVKDYQAAYKAVPDALAALTWDACAIAESALNASIKKVGKLDRNALRDA
                     LAATKDAHGVSGKMAFDQNRNPIKPAVILEVKDGKFAYKATVNP"
BASE COUNT         1054 a         1610 c         1417 g         1059 t
ORIGIN      
        1 gcgcactgtc gcccgatggg ataagaccga agtagtcgat gatcaccgcc gccgccctag
       61 ccccgagcct tccggccttg cgttcaaggt cttcccagtc caccgccagg tcggcatcga
      121 ctcggtaata ctcgatcctc actcccatcc tgcgaaaggg catgagtatc gaatagcaca
      181 ggtaggacgg gagcagcact gtgtcgaggc cgtgctctgc gtgcagcagg ttcagccata
      241 cgtttagata ggcagtgcgt ccgctggaca tgaggagtgt gggatacccc cgggaccaac
      301 cgtcgattag gtccgccaca ctcgcctcac cgtctggagc gcggccgatg tcagcgaaac
      361 agaattcccc gcctatctcc attcacatgc cccttccagt atcaggcgct ccctagatac
      421 gcagcctgga cccgagagtc ttttgccagt tcggcggcgg atccctgcag ggctatgctg
      481 ccggtttcga gcacgtaggc acggttcgcc actgccagcg ccatagaggc gttctgttct
      541 accagcagga tcgcagtgcc cgactggttt atcctcacta tcacctggaa gatctcctta
      601 acaagaaggg gggcaagccc cattgacggc tcatctagga ggagcaggtc cggccttgac
      661 atcagccctc tccccattgc tagcatctgc tgttcgccgc cggacaaggt cccggccgct
      721 tgagcacgtc tttcacgcag cctggggaag agctcgaaca ccatctcaaa gtcccgcttt
      781 atctcggcct tgtcgtgcct gaggtacgcg ccgagctcta ggttgtccat gaccgtcagg
      841 ttggggaaga tcctcctccc ctcaggcact tgcgatatcc ccaggtccac aagcttgtga
      901 gcaggcagcg agcacagatt cacctgtcgc cccgccgcgt cggctttgct ggtcgccggc
      961 ggaacccagt acgacataga accatccgta ggacgtatca ggccggatat ggttcggagg
     1021 accgtgctct tcccggcgcc gtttgcgccg attaaggtaa cgatctcacc gcgatcgaca
     1081 ttgaacgaaa tgcctttcag cgcatggatc gcgccgtaat gaacgtggag attctcaata
     1141 cccaggagca tcagaccgcc tcctctccca gatacgcctc gatgacgcgt ctgttcttaa
     1201 caatctcggc aggggaaccg tcggctatgg tgacgccgta atcgaggacg atgatacgct
     1261 gacacagctt catcaccagg ttcatgtcgt gctcgatcag aagcacggtc agatcgaacc
     1321 tttcgtgaat ccacctgatt agctcgagga gttcctgagt ctcctgaggg ttcatgcccg
     1381 ccgcaggctc gtcgagcagc agcagtctcg gttttgtcgc cagagcgcgg gccatctcaa
     1441 gtctcctctg cgcgccgtac ggaagcttgc tggcgatctc cgatgcccgg tcttccagcc
     1501 cgaacagccg gagaaactcc atggacttgc ggtatatctg gtcttcctga gccgaatagc
     1561 gacccgcccg gagaagcgcc tcagtcatgg agtaagggcg cgctccgcga aacgcaatac
     1621 gcacattgtc cagaacagaa agattactga aaagacggat gttttggaac gtgcgagcaa
     1681 tgcccatatc gttgatctct gacggcttac gccttgttat gtcctctccc atgaagtgga
     1741 tgcgcccgct cgtggcttcc gcaaggcctg ttatcatatt gaagacagta gtcttgcctg
     1801 cgccgttggg cccgatcagt cccaccagtt cgccctggca gagcgtcatg ttgaagtcgg
     1861 acaccgccct aagtcccccg aaatccatgc acactcggtg ggtctcaagc acgggaggcg
     1921 ggccgtcgcc cgtcgccgcc acctgcccgc ccccgtcggg aacagggcgc ccgttgccaa
     1981 gcccgagttg tttgaagctg aactcggcgg tgcccatgag gcccctgggc ctgtatagca
     2041 tcatgaggat cagcatgagg gagtagatga tcatcctgta atcggcgaac gcgcgcagga
     2101 tctcaggaag gaatgtcacg acgaaagccg ccatcaccgt tccagtgatg ctgcccaggc
     2161 cgccaaggac tatgatcacc agggcctcga ttgacttcat gaagtcgaac tgactcgggt
     2221 cgatgaaggt tatcttgtgg gcatacaggc ctccggccac gccggcgaag aaggccccca
     2281 ctacgaacgc cattatcttg ttgcgggtcg tgtcgacccc catggtctcg gcagcggttt
     2341 cgtcttcgcg tatcgccacc agagcccgtc cctgcctcga ccagatcagg tttctcacta
     2401 cgaccacggt cgccgccgct atcacgtaca cccacagaaa ggtcgtatgc gccgggatcc
     2461 ccggcagccc tctcgggcca cccaccacgt ccaggttgag tatgatcact cgaatgatct
     2521 cgccgaaccc cagtgtggcg atggccagat agtcacctgc cagcctcaat gtggggatgc
     2581 ctaccaccaa tccgcccgcg gccgcggcga ttccgcctgc aagcagcgcg agagcaaacg
     2641 gcatgcccat cagcttggtc atggatgcag ctacgtaagc gcctatggcc atgaagcccg
     2701 catgccccag ggagaactgc ccggtgaacc cgtttatcag gttgaggctg actcccatga
     2761 tgacgtttat gccaacccag atgagtatct ggaggtagta gtcgttgatc aagatgtctg
     2821 cacgatgcag cgccactaca aggaagtacc cggccgcgag ccctcctagc gtcacagcgg
     2881 cgcgggcaag acgtctgccg tcggttgcct tagcattggt tttcgttcgt tttgcgcttg
     2941 ccgtgtctgc ctcagccatt tctgcccacc tacactttct ccctgacggc ccgcccaagc
     3001 agacccgcag gtttgaacag caagatgacg atcaggatcg cgaaggctat cgcgtccttg
     3061 agcgtagacg acacaaaggc tatcacgaac acctcggcga ctcccattat cagcccgcct
     3121 agcatcgcgc ctggcactat gccgatgccg cctaccacag ctgccacgaa tgctttgagg
     3181 ccgtacatgg tgcccatagt cggagaaatg cgattatagt agaagcccac cagcatcccg
     3241 cctgcggctg ccaaagccga gcccagcgcg aaggtcacgc tgatgaccat gtccgcatcg
     3301 atccccatga gcctcgccgc atccttgtcg aatgaaacag ccctcatcgc cttgccgatc
     3361 ttggtacggt acacgattag gctaatcagc accatgagcc caatggccag ccccaacatg
     3421 atgagttgca cgttcgacac taccacgccg cccacgtccc acactaccat gctcattatc
     3481 ctcgggtatg gcctgggcct ggcctcgaag aacttcaaac ccaggttctg gaggagcagc
     3541 gataccccga tcgcggttat gagtgacgcg atgcgcgggg cctgcctgag aggtttgtac
     3601 gccaaccgct cgactgccat gcccgtcaca gctgccagaa tcatcgtcag aggcagagca
     3661 agcgcgaatg gtatgttgaa cattgtgatg gcgaagtaac cgtagtaagc cccgagcatg
     3721 aatatgtctc cgtgagcgaa gttgatcaga cgtatgattc catataccat ggtgtagccc
     3781 agggcgatga gcgcatacac gctgcccagg gctaacccgt tgactagctg ctgtataacc
     3841 atgctgatat ccaggccgcc agacaaagat aatcacctct actacgaaag caggggggcg
     3901 gcgaagtgcc cccctgcccg tcgcttccgg atgctgttat cgctcaagcc cacgcgttca
     3961 aagttgcgtt tgaaggcgcc ggatcacgga ttgaccgtcg ccttataggc aaacttgcca
     4021 tccttcacct cgaggatgac cgcgggtttg attggattgc ggttctggtc gaaggccatc
     4081 ttgccggaga ccccatgcgc atcctttgtc gcggcgaggg cgtcgcggag ggcattcctg
     4141 tcaagcttgc cgaccttctt tatcgaggcg ttcagcgcgc tttcggctat tgcgcacgca
     4201 tcccacgtca gcgcagccag cgcgtccgga acggctttgt acgcagcctg atagtctttc
     4261 acgaaggcaa cagccacagg gtcagtcgaa ccggggtagt agtggttgct gaagaacccg
     4321 ccctcaatgg aatcgcctgc gatgggtatc aactctgacg aatcccatcc atcggcgccg
     4381 aggaagacag ttctcttgat gccgagctgc cgggcttgcc tggtaatgag gccgactgtg
     4441 ttgtagtagt cgggaagcca cagcacgtcc gggtcggccc tcttaatctt ggtgagctgt
     4501 gcggagaagt ccgattcgcc cacgccgtac gtctcgctca caactacctt gcctccgccc
     4561 gcctcgaaga acttcttgaa attctctgcg agccccttgg tgtagtcatt gcctatgtcg
     4621 tagagcatcg cagcggtctt ggctttcagg gtctcgagag cgaacttgcc catgacctgg
     4681 ccctggaatg gatcaatgaa acaggctctg aagacgtagt tgccgaccgc ggttactgcc
     4741 tggtcggtgg cagtcggggt aagaaccggg accttgctgg cctgaccaat gggggcacca
     4801 gcgttagcgc acttgctggt aacagggccc atgatcataa gtaccttgct ctggttgatc
     4861 agtttcgaca ctgcgttggc cgtttcagac ggcacgccct tgtcgtcttc cacgattatc
     4921 ttgaccttgc ggccctggat gccgccgcgg ctgttgacct gcttctccca gaggagagca
     4981 gcgttcctca cggacattcc gaaagtcgca acgtcgccgg tgaccggcgt gactaggccg
     5041 atcacaatct cctcggcccc cagcgcaaca gcgctcatga taagcaggac cgagaccacc
     5101 aatactaggg actttctcaa caactgcctc tcctcctcac
//
LOCUS       DAOTAC010000186         5130 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_13544_length_5130_cov_4.822266, whole genome shotgun sequence.
ACCESSION   DAOTAC010000186 DAOTAC010000000
VERSION     DAOTAC010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5130
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13544_length_5130_cov_4.822266"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="PLM74_08325"
     CDS             <1..409
                     /locus_tag="PLM74_08325"
                     /inference="COORDINATES: protein motif:HMM:NF015085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HON43117.1"
                     /translation="DEDRHCPEKAGGTQVVKKRLVLTILIVAAFAVFAAGAFRNALTP
                     YVTFAEAAQRGGSVQVSGVADKDAARMDSESGMFLFDLTDRSGQTMPVQFAGAPPGNF
                     DEAESVVVVGNLHDGVFEARKILVKCPSKYQAK"
     gene            520..1833
                     /locus_tag="PLM74_08330"
     CDS             520..1833
                     /locus_tag="PLM74_08330"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HON43118.1"
                     /translation="MDAVKIGLLGLGNVGSGVWKILNDNADEIAEKVGARLQIAKVLV
                     HDPDKGRKVSVPRGLLTTDADSIVNDPDIPIIVETIGCPGQDTQLARDYMLRALRAGK
                     HVITANKEVLARHGRELRAAVTDGGGLYYEGAVAGGIPVVKVFKEALAGNKALSLMGI
                     INGTTNYILTRMSQEGSDLDEVLRMAQSLGYAEQDPTSDVDGHDAAYKLALLASIAFG
                     HDVQVEDVFREGISRIKLEDLCYAAELGYAVKLLAIARRDGDGDGDGDDSVQLAVHPT
                     MIPHSHPLASVGGVFNAVFIRGDAVGDLMLYGMGAGMMPTGSAVVADCIDAAHNFIRR
                     VSPPREVRRNHVHVKPMDETESRYYVRVLVVDRPGVMAAIAAEFGRAQVSIESLIQKG
                     RGQDPVSVVFVTHRARERDIQDALREIEALDVVHSIASVIRVEAS"
     gene            complement(1879..2520)
                     /locus_tag="PLM74_08335"
     CDS             complement(1879..2520)
                     /locus_tag="PLM74_08335"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010291725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HON43119.1"
                     /translation="MEIRVSPTRMQLQQLKKRHAMAVRGHKLLKDKLDELMKHFLELV
                     RRDRDLRAQIDDRLGAAFRGFLIARGAMSAAAVDTALIYPKETISLRAGQRHIMNVAV
                     PSLEWTFEHEGDATNIYPYGFAYTSTELDSAIRLLSEAMPSLVELAEVEKAVELLADE
                     IEKTRRRVNALEYVLIPSLEQSIKYITMKLDEAERGNLTRLMKVKEIVGERGA"
     gene            complement(2522..3910)
                     /locus_tag="PLM74_08340"
     CDS             complement(2522..3910)
                     /locus_tag="PLM74_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HON43120.1"
                     /translation="MLKEYLTVREVAGPLMLVEQVQDVKYGELVEIAVRGEIRRGRVL
                     EASGTNALVQLFEGSAGLEIKSARVRFLGHGIELGVSPEILGRVFDGLGRPRDYGPKI
                     IPDARLDTNGAPINPYARDYPSEFIQTGISAIDGLNTLVRGQKLPIFSGAGLPHARIA
                     AQIARQARVLGTEEQFAVVFGAMGITFEESDFFISDFKRTGAIERAVLFINLANDPAI
                     ERIATPRMALTAAEYLAYDLGMHVLVILTDMTNYCEALREVSAARKEVPGRRGYPGYL
                     YTDLATIYERAGRIKGNKGSITQFPILSMPEDDKTHPIPDLTGYITEGQIMLSRELHR
                     KGIYPPIDVLPSLSRLKDKGIGSGKTREDHADTMNQLYAAYARGKEAKELAVILGEAA
                     LSAVDKIFSKFADEFERRYITQGEEEDRPIEQTLNIGWDLLSLLPVDELTRVREEFIE
                     KYLPKAGEDEAE"
     gene            complement(3903..>5130)
                     /locus_tag="PLM74_08345"
     CDS             complement(3903..>5130)
                     /locus_tag="PLM74_08345"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HON43121.1"
                     /translation="EITMMQKWPVRRGRPIGQKLTPGQPMVTGQRVLDTFFPVAKGGT
                     ACIPGPFGSGKTVVQHQLAKWADADIVVYVGCGERGNEMTDVLMEFPELLDPKSGEPL
                     MKRTVLIANTSDMPVAARESSVYTGITIAEYFRDMGYSVALMADSTSRWAEALREMSG
                     RLEEMPGEEGYPAYLGSRLAEFYERAGRTTCLGADGREGALTVVGAVSPPGGDLSEPV
                     TQNTLRVVKVFWALDANLAYSRHFPAINWLTSYSLYLDRVDADLREMVGDEFVEMRGE
                     AMRILQDEAGLAEIVRLVGMDALSTRDRITMETARIIREDFLHQNAFHEVDTYTSLAK
                     QAAMLRVILEMDRAAREAVERGASLDEVLALPVRAKIGRMKYVEEARLSEIETMVDEV
                     RRSVGAIAAGGGEDVA"
BASE COUNT         1019 a         1588 c         1533 g          990 t
ORIGIN      
        1 tgatgaagat cgacactgcc ctgaaaaagc tggagggacg caagtagtga agaagaggtt
       61 agtcctcact atcctcatag tagcggcttt cgccgtgttc gcggcgggtg cgttccgaaa
      121 cgcgctcact ccgtatgtga cttttgctga agctgcgcag cgcgggggga gtgtgcaggt
      181 atccggggtg gccgacaagg atgcggcccg gatggattcc gaaagcggaa tgttcctgtt
      241 cgacctcacc gaccgctcgg gccagaccat gcctgtgcag tttgccggcg cccctccagg
      301 caatttcgac gaggccgaaa gcgtggtcgt agtagggaat ctacacgacg gggtgtttga
      361 agcccgaaag atcctggtga agtgcccgtc taagtatcag gccaagtaga acccccaaat
      421 cgcagctgct gcagctcgtc tacggccatg accgtgtatc tttttccgcg gctacacgct
      481 ataatcggaa gaaatgcacg ctatctagga ggaggcgccg tggacgctgt aaagatcgga
      541 ctactgggac tcggaaatgt cggcagcgga gtttggaaga ttctgaatga caacgcggac
      601 gagattgcgg agaaggtcgg agcccggctg cagatagcaa aggtgctggt acacgacccc
      661 gacaagggac gcaaggtcag tgttcccagg ggacttctga ctaccgacgc ggacagcata
      721 gtgaacgatc ccgacattcc catcatcgtc gagacgatag gctgcccggg ccaggacacc
      781 cagctcgccc gcgattatat gttgagggcg ctcagagccg gcaagcacgt gataacggct
      841 aacaaagagg tgctggccag gcacgggcgc gaactacggg ctgcggtgac cgacggcggc
      901 gggctatact acgaaggcgc agtggccggc ggaatccccg tcgtcaaggt gttcaaagag
      961 gccctggccg gcaacaaagc gctctcgctc atgggcataa tcaacggcac caccaactac
     1021 atcctaacgc gaatgagcca ggaggggtcg gacctcgacg aagtgctgcg aatggcgcag
     1081 tcgctggggt acgccgagca ggaccccaca tccgatgttg acggccacga tgccgcctac
     1141 aaactggcac tactcgcttc gatcgctttc ggccacgatg tgcaggttga ggacgtcttc
     1201 cgcgaaggca tctcccgcat caaactggaa gacctgtgtt acgccgccga gctgggctat
     1261 gcagtcaagc tgctggcaat agccaggcgc gacggagacg gagacggcga cggcgacgac
     1321 agtgtgcaac tcgcggtgca ccccaccatg atcccacata gccatccact ggccagtgta
     1381 ggcggggtgt tcaacgctgt gttcatacga ggcgacgctg taggcgacct gatgctgtac
     1441 gggatgggag cggggatgat gcccacaggt agcgccgtgg tagccgactg catcgacgcg
     1501 gcgcacaact tcatccgccg ggtaagcccc ccgcgggaag tccggcgaaa tcatgtgcat
     1561 gtcaagccca tggatgagac agagtcacgc tactacgtga gagtgctggt agtggaccgg
     1621 ccgggcgtga tggcggcaat agccgcagaa ttcggccgcg cccaggtaag catcgagtcg
     1681 ctcatacaga aaggacgagg ccaggacccc gtcagtgtgg tattcgttac ccacagggcg
     1741 cgcgaacgag acattcagga cgctctgcgg gaaattgagg cgctggacgt agtgcattcc
     1801 atcgccagcg tcatcagggt cgaggcgagt tgaaagacga agaccggaga ggaagaccgg
     1861 aaaagacgag cgcgggttct acgccccgcg ctctccaact atctctttga ctttcatgag
     1921 cctggtcaag ttgccgcgct cggcctcatc cagtttcatg gtgatgtact tgatggactg
     1981 ctctaggctg ggtatgagga catactccag cgcattcacc ctccgccggg tcttctcgat
     2041 ctcgtccgcc agaagctcca ctgccttctc cacttcggcc agctccacca ggctgggcat
     2101 tgcctccgac agtagcctga tggctgagtc cagctcagtc gatgtgtagg caaaaccgta
     2161 cgggtatatg ttagtggcgt cgccctcatg ctcaaaggtc cattcaaggc taggaacggc
     2221 tacgttcatg atatggcgct gcccggccct gagggagatc gtctccttgg ggtatatgag
     2281 cgccgtatcc acggcggcgg ccgacatcgc cccccgcgcg atcaagaacc ctcggaaagc
     2341 cgcgccgagc ctgtcgtcga tctgcgcgcg cagatcacga tcgcgccgca cgagctcaag
     2401 gaagtgtttc atcagctcgt ccagcttatc cttgaggagc ttgtgcccac gaacggccat
     2461 ggcgtgcctc ttcttgagct gctggagctg catccgggtg gggcttactc tgatctccat
     2521 ctcactcagc ctcgtcctcg cccgccttgg gcaggtactt ctctatgaat tcctctctca
     2581 cacgagtcag ctcatccaca ggtagtagcg aaaggagatc ccagccgatg ttgagagtct
     2641 gctcaatggg acggtcctct tcctcgccct gtgtgatgta gcgccgctcg aactcgtcgg
     2701 cgaacttgga gaagatctta tcaacggccg acagcgccgc ctcgcccagg atcaccgcca
     2761 gttctttggc ctctttgccc cgggcataag ccgcatagag ctggttcatg gtgtcggcat
     2821 ggtcctcgcg tgtcttgccg ctgccgatgc ctttgtcctt aagccgcgac aacgagggca
     2881 gcacgtcgat gggcgggtat atgccctttc ggtgcagctc gcggctgagc atgatctgcc
     2941 cctcggtgat gtaccctgtc aggtcgggga tggggtgagt cttgtcgtct tcaggcatcg
     3001 aaaggatcgg gaactgggtg atcgaaccct tgttgccctt gatccttccc gcccgctcat
     3061 atatggtcgc aaggtcggtg tagaggtacc ccggatagcc acgccttcca ggcacttctt
     3121 tacgtgcagc cgacacctcg cgcagcgcct cgcagtagtt ggtcatgtcg gtcaggataa
     3181 ccagaacgtg catgcccaga tcgtatgcga gatactcggc agcggtcaga gccatccttg
     3241 gtgtggcgat ccgctcgatg gcagggtcgt tcgcaaggtt gataaagagc accgcgcgct
     3301 cgatggcgcc tgtacgcttg aagtcggaga tgaagaagtc cgactcttcg aatgtgatgc
     3361 ccattgcccc aaagaccact gcgaactgct cctccgtacc cagcaccctg gcttgccgcg
     3421 caatctgcgc ggcgatgcgc gcatgcggca ggcctgcgcc tgagaagatc ggcagtttct
     3481 gcccgcgcac cagtgtgttc aggccgtcga tggcggatat gcctgtctgg atgaactcag
     3541 acgggtagtc gcgcgcgtag gggttaattg gagcgccgtt ggtatcgagc cgagcgtcgg
     3601 gtatgatctt agggccgtag tcgcgcgggc ggccaagacc gtcaaacacc cttcccagaa
     3661 tctcgggcga cacgccaagt tcgattccgt ggcccaaaaa acgcaccctc gcggacttga
     3721 tctcaaggcc ggcggatccc tcgaacagct ggactagagc attcgttcct gacgcttcca
     3781 gcactcggcc gcgcctgatc tcgcccctca ccgcgatctc cacaagctcg ccatacttaa
     3841 cgtcctgtac ttgctcgacg agcatcagcg ggcccgcgac ctctcttaca gtaaggtatt
     3901 ccttaagcaa cgtcctcacc tccgccggca gcgatcgcgc ccacgctgcg cctgacctcg
     3961 tccaccatag tttcaatctc ggagagcctg gcctcctcaa cgtacttcat ccgcccgatc
     4021 ttggcgcgga cgggcaaagc cagcacctca tcgagcgatg ctccgcgctc gactgcctca
     4081 cgtgcagcgc ggtccatctc cagtatcact cggagcatcg ccgcctgctt cgcaagggac
     4141 gtgtaggtgt ccacctcatg gaacgcgttc tggtgcagga agtcctcgcg gatgatcctg
     4201 gccgtctcca tggtgatcct gtcgcgagtc gacagcgcgt ccatccccac gagtcgcacg
     4261 atctccgcga gccccgcctc atcctgcaat atcctcatgg cctcgccgcg catctcaacg
     4321 aactcgtcgc ccaccatctc gcgcaggtca gcatcgaccc tatcgaggta caacgagtag
     4381 ctagtaagcc agttaatggc cgggaagtgg cggctatacg ccagattagc gtcgagcgcc
     4441 cagaagacct tgaccactcg caacgtattc tgagtaaccg gctccgacaa gtcgcctccg
     4501 ggaggcgaca ccgcgccaac aacagtgagc gcgccttcgc gtccatcggc ccccaggcat
     4561 gttgtgcgcc ctgcccgctc atagaactcg gccaagcgcg agccaaggta ggccgggtag
     4621 ccctcctcgc ccggcatctc ctcgaggcgc cccgacatct cgcgcagagc ctcggcccag
     4681 cgcgaggtgg agtcggccat gagggcgacg ctgtagccca tgtcgcggaa gtactcggcg
     4741 atcgtgatgc cggtgtagac agacgactca cgggccgcaa ccggcatgtc ggacgtattc
     4801 gcaatcagca cggtcctctt catgagcggc tctccgctct tggggtcaag gagttcaggg
     4861 aattccatga gcacgtcggt catctcgttg ccgcgctcgc cgcagcccac gtagaccacg
     4921 atgtcagcgt cggcccactt ggccagctgg tgctggacaa cggttttgcc agaaccgaag
     4981 ggccccggaa tacaggccgt gccgcccttg gccactggga agaacgtgtc gagcactcgc
     5041 tgcccggtca ccatcggctg cccaggcgtg agtttctgcc cgatggggcg accccgcctt
     5101 accggccatt tctgcatcat cgttatctca
//
LOCUS       DAOTAC010000187         5129 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_13549_length_5129_cov_4.263303, whole genome shotgun sequence.
ACCESSION   DAOTAC010000187 DAOTAC010000000
VERSION     DAOTAC010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5129
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13549_length_5129_cov_4.263303"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="PLM74_08350"
     CDS             complement(<1..872)
                     /locus_tag="PLM74_08350"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HON43122.1"
                     /translation="MNESNGSAGFCFVHASDVHLDSPFSSVKLDSPEVAEAFAGAATA
                     SLQGIARVCIEEGARFLVLSGDVFDSEQRSLRAQHELWAVLSSLAERGVHTYVAFGNH
                     DSLSGWRSAFSWPEEVHFFGPEVSTYPVIAGGREIARVSGISYPQPSVADNLSLAFRK
                     DPGDPFTVGVLHCNVGGDVRHANYAPCTLADLDASGIDYWALGHVHEPRVLRTDCPAV
                     VYSGCVQGRNPRETGAHGCFVVRVAGDGGGSEVGGGRVALKFVPTDVIRWHVLDVMID
                     GLSTVDQVIDEIQS"
     gene            complement(976..2124)
                     /locus_tag="PLM74_08355"
     CDS             complement(976..2124)
                     /locus_tag="PLM74_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43123.1"
                     /translation="MKSRQSVWRALWPAPLSTLRSVLRSTLSVLRSTLPALALAVCLV
                     SSAVCVCATSATSAPATLGLTPEALYELSMAPTSDYEARIRMWNPSTAEVWVDAARAD
                     GITVYSMCASGYIASLALADGMRVFYGTPLNGTSSGSPADMTRFPAAGPRIQALASLA
                     GPLRYDSALMSENYTFTACQGASVAGRDTIELRIDPKWPGNVRRTVWVDFYAGTALRV
                     EDRNYRGDLIWLWEVERIAFGPPRRQIMQAARAALSSDEAVPYSEPSMSLGEISRAAG
                     FRVTLPAWVPEGYRLVALRPAQGILDREEPGPGRLRLMARMTSVAQLIYSDGLGAITF
                     YQRPVPWWARRASAPRVGHAIEWERNGIRYVLVGDVDPQLLLKMARSL"
     gene            complement(2133..2675)
                     /locus_tag="PLM74_08360"
     CDS             complement(2133..2675)
                     /locus_tag="PLM74_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014821484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HON43124.1"
                     /translation="MKPTVGGNTRGRDGALLHLDDESLGAYVDGELHGWAKQAAADHL
                     RTCSACRAAADAIAGLGSAARRLESPEWDVEAMVALVDAGISRTGVVGVACASRSRAA
                     KTVRRESWVGRAAGWIRSHRRLALGLGVAVIVLAVSALGIAETAYRRELTAQDKLLVD
                     SHYIIRSGNVGALLVSYPGR"
     gene            complement(2696..3313)
                     /locus_tag="PLM74_08365"
     CDS             complement(2696..3313)
                     /locus_tag="PLM74_08365"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HON43125.1"
                     /translation="MVGSEWARMSDGDLARRAASGSQDAYSELVRRHSKKLLNLAWRM
                     IGSEDAAWDAVQEALLAGWRGISNFRGEAEFYSWIRRILVNAIMGQFRKEGRNEAAMV
                     SLDMPAGSGEAGEPLHVQVEDEGSDPQDVVGRIETAREIGRAVARLPDIYRTVFLLRE
                     TELMSYSEIAGVLGIAEGTVKSRLNMARRMLREALGEEVKAARFA"
     gene            complement(3517..4356)
                     /locus_tag="PLM74_08370"
     CDS             complement(3517..4356)
                     /locus_tag="PLM74_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43126.1"
                     /translation="MKRILVLLILVCLALGSAASVYADGIDESQPSALPEVAAPEPVQ
                     PGEAAPKSAEQLPRGTERGPIRGRVKVLVPMPAPSPRSGEWGQCPLCGSTSKAEQELA
                     IWAKVTGVPAEELKKHLSSGVGLGEICGAVVVARKQGKSLGQVIQQAKTDKVSMPVLA
                     YAAGMSMEEFSREIAALHTAFLEQAAADGLITKDQVTKDQVTKGQEALAPDRLRRRLV
                     DSRKAGIQMGLKPGLGMQYRVMPNLGSVGQQMIRFAGMRTMILMHWRNMMRGPVSLPS
                     NGK"
     gene            4602..>5129
                     /locus_tag="PLM74_08375"
     CDS             4602..>5129
                     /locus_tag="PLM74_08375"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HON43127.1"
                     /translation="MTYLGRPVLMQRRAPLTYALLATNILVFIALEASGGSQNPLVLL
                     KWGAKFTPAIRAGQPWRLFTAMFLHAGVLHLAVNSYSLHNLGNVVERLFGTHRFALIY
                     LIGGVWGSLASTFMSDPRVLGVGASGAIFGLAGCLFYFGLRYPSHFRVLAGWRFIAIV
                     LINLLIGFSSSHIDGY"
BASE COUNT         1017 a         1728 c         1367 g         1017 t
ORIGIN      
        1 ctgctctgga tctcgtcaat aacctgatca acagtggaca gcccgtcaat cataacgtct
       61 agcacatgcc agcggatgac gtcagtgggc acgaatttca gtgccactct cccgccgcct
      121 acctcgctcc ctccaccgtc accggccaca cgtaccacaa agcagccgtg cgcccccgtt
      181 tcgcgcgggt tcctcccctg cacacacccg gagtacacca cggctgggca atcggtgcgc
      241 aaaacccgcg gctcatgcac atgcccgagc gcccagtagt cgatgcctga tgcgtcaagg
      301 tcagccagtg tgcagggcgc atagttggcg tgccgcacat cgcccccgac attgcagtgg
      361 agaacgccca ctgtgaacgg atcacccgga tctttccgga acgcaagaga cagattatcg
      421 gccaccgacg gctgtgggta gctgatcccg ctgacccggg cgatctcgcg cccgcctgcg
      481 ataaccggat atgtagagac ctcgggcccg aagaaatgga cctcttccgg ccagctgaag
      541 gcggatcgcc atcctgatag actgtcgtgg tttccgaaag ctacgtaagt gtgcaccccg
      601 cgttcagcta gactgctcag gaccgcccac agctcgtgct gtgccctcaa gcttcgctgc
      661 tcgctgtcga atacatcgcc tgacagcact aggaagcgcg ccccctcctc gatgcagacc
      721 cgcgcgattc cctgaagcga agccgtggct gcaccggcaa aggcctccgc cacctctggg
      781 gagtctagct taaccgacga aaacggggaa tcgaggtgga catcggaagc atgtacgaag
      841 cagaacccag ctgacccatt ggactcgttc aaacagaaga ccccctccac gacgattatt
      901 cgcgcctgcg agggggttga ccttccggtg ttggagcgcc tgcatctccc ggcatttcag
      961 gcaatcatgg ccatttcaca gacttcgggc catcttcagc aatagctgcg ggtccacatc
     1021 gcccacgagc acgtagcgta tgccattgcg ctcccactcg atggcatgac ccactcgtgg
     1081 agcagatgcc cgtcgggccc accacggaac cggtcgctga tagaatgtga tcgcgcccag
     1141 accgtcgctg tatattagct gagccaccga cgtcatgcga gccatcagtc gcaacctccc
     1201 ggggccgggt tcctcccgat caagaatccc ctgggccggc ctcaacgcaa cgagccggta
     1261 accttccggc acccatgctg gcagcgtcac cctgaacccg gccgctctgg atatctcgcc
     1321 cagcgacatg ctcggttccg agtaaggcac tgcctcatcc gacgatagcg cagcgcgtgc
     1381 cgcctgcatt atctgacgac gcggcggccc gaacgcaatc cgctcaacct cccacagcca
     1441 gatcaaatcg ccgcggtagt tgcggtcctc cactcgcaaa gcggtgccgg cataaaagtc
     1501 cacccatacc gtcctgcgca cattcccagg ccacttagga tcaatccgca actcgatggt
     1561 gtcgcgtccg gcaacggaag cgccctggca tgccgtgaac gtgtaatttt ctgacataag
     1621 cgccgaatcg tagcgcaggg gtccagccag cgatgccaga gcctgaatgc gtggccccgc
     1681 ggccgggaag cgagtcatgt ctgcgggtga gccggaggag gttccattca gaggcgtccc
     1741 gtagaagacc cgcatcccat cggccagggc caaactggcg atgtacccgg acgcacacat
     1801 gctgtagaca gtaatgccgt ctgcgcgagc cgcatccacc cacacttccg ccgtggacgg
     1861 gttccacatc ctaatccggg cttcgtagtc agacgtcggc gccatactga gttcgtagag
     1921 cgcctccggc gtaagcccga gagtggctgg agcagaagtc gcagatgtgg cacagacgca
     1981 tacggcagat gacaccaaac agacggcaag tgccagtgcc ggcagtgttg accgcagcac
     2041 cgacaatgtt gaccgcagta ccgacctcag tgtggacaat ggtgctggcc atagtgcccg
     2101 ccatactgat tgccgcgact tcactgctca gttcacctcc ccgggtacga aactagcaac
     2161 gctccgacat tcccggaacg gatgatgtag tgcgaatcca caagaagctt gtcctgtgcc
     2221 gtcagctcgc gtctgtatgc agtttcagcg atgccaagtg ccgacaccgc cagcacgatc
     2281 acagcaaccc ccagcccgag tgcgagtcgc ctgtgagatc tgatccatcc tgccgcccga
     2341 cctacccaac tctctcggcg cacggtcttg gccgcgcggc tacgcgatgc acaagccacg
     2401 cccacgaccc cagtcctgga gatgcccgca tctactagtg caaccatcgc ctctacatcc
     2461 cactcgggcg attccaagcg ccgtgccgca cttccgagcc cggctatggc gtcggccgcc
     2521 gcacggcacg cagaacacgt ccgtaggtga tcggctgcag cctgctttgc ccacccatgt
     2581 agttccccgt cgacgtacgc gcccagcgat tcgtcgtcca gatgtagcag ggcgccgtca
     2641 cgcccgcgcg tgttaccgcc tactgttggc ttcatgcacc atgccgcgcc ggctctcacg
     2701 cgaaccgcgc agccttcacc tcctcgccca gagcctccct gagcatccgc ctggccatgt
     2761 tcagcctgga ttttacggtt ccttctgcaa tcccaaggac gccggcgatc tcagagtagc
     2821 tcatcagttc cgtctcacgc aggaggaaca ccgttctgta gatgtcgggc agtcttgcga
     2881 ctgccctacc tatctcccgg gcggtttcga tccggcccac aacgtcctgg ggatcagaac
     2941 cctcgtcttc cacctgaacg tgaagaggtt cgcccgcctc acccgaaccc gctggcatat
     3001 ccagcgacac catggcagct tcgttccgac cttccttccg gaactggccc atgatcgcgt
     3061 tcacgagtat ccgccggatc caggagtaga actcggcctc gcctctgaag ttgctgatgc
     3121 ccctccatcc tgcaaggagg gcctcctgca ccgcgtccca cgctgcatct tcagaaccga
     3181 tcatccgcca tgccaggttg agcagcttct tcgaatgcct ccgtacgagc tcagagtagg
     3241 catcctgcga acccgacgct gctcggcgcg caagatctcc atcggacata cgtgcccact
     3301 ctgaaccgac caacagcccg accccctgcc gcccatggga tggatcccgg agtcggcgct
     3361 acgcaagccg ggtcgatcgg cccgactccg ggatctccat caatgcgtcg accaatgcct
     3421 cggctgatgc ctcgacactg catcagcaat acgccgactc gcacgtttag cgagacattc
     3481 gacaggctcg ctgtacagac cttcggacat ggaggatcac ttgccgttgc taggaaggct
     3541 cacggggcct cgcatcatgt tcctccagtg cataagtatc atggtgcgca tgccggcaaa
     3601 ccttatcatt tgctggccga ccgaccccag attgggcatc acgcgatact gcatgcccag
     3661 ccctggcttg agacccatct gaataccggc cttgcgtgag tcgacgagtc gccgcctcag
     3721 gcgatccgga gccagtgctt cttgcccctt ggtgacctga tccttggtga cctgatcctt
     3781 agttatcagc ccatctgcgg cggcttgctc aagaaaggct gtatgtaggg cggcaatctc
     3841 cctagaaaac tcctccatgg acattcccgc agcgtacgcc aggacaggca tgctcacctt
     3901 gtcggtcttt gcctgttgta tgacttgccc caggctcttg ccctgcttac gtgcgactac
     3961 taccgcacca catatctcac cgagcccgac tccgctcgac agatgcttct tgagttcctc
     4021 ggctggtacg ccagtgacct ttgcccatat cgccagttcc tgttcagcct tgctggtact
     4081 gccgcacagc gggcactgcc cccattcacc ggaccttggc gaaggcgcag gcataggcac
     4141 aagtaccttg acacgaccgc ggatcgggcc tcgctcggtc ccgcgcggta gttgctctgc
     4201 agacttcggt gcagcttccc caggctgcac aggttcgggt gctgccacct cggggagggc
     4261 agacggttgg ctttcatcaa tgccgtctgc atatactgat gccgccgatc ccagagccag
     4321 acataccaga atcaggagaa caagaatgcg cttcactaca gtcactcctt tacgctaatg
     4381 tagctggcga tgcgcgcatc cggcacgtat cgctcaggcc ctatttgtac tgatgctgag
     4441 agcgggcaga agttcgaata cgaaaatcgc aggcgtttcc gcgcatactg ggcattgcgc
     4501 gtgacaatga atagcgcatc acgcatgata agtatgcctt gcccgaaacc actcatgtcg
     4561 tgtactatct gcagtaggat tcatttggag gtacttgacc catgacatac ctcggccgac
     4621 ctgtactgat gcaaaggaga gctcctctga cctacgctct ccttgcaact aacatactgg
     4681 tcttcatcgc cttagaagca tctggtgggt cgcagaatcc cctggttctg ctgaaatggg
     4741 gcgccaagtt cacccccgca atccgcgccg gccagccctg gcgccttttc acggccatgt
     4801 tcctgcatgc cggtgtgctc catttggccg tcaactccta ctctctacac aatctcggga
     4861 acgtggtgga gcgcctattt ggcacacacc ggttcgccct aatctacttg ataggcggag
     4921 tatggggttc gcttgccagt acgttcatgt ccgaccccag agtgctcgga gtgggcgcct
     4981 cgggggccat attcggcctc gccggctgtc tcttctactt cggtctccga tatcccagtc
     5041 atttcagggt gctggcaggc tggagattca tagcgatcgt cctgatcaac ctgttaatcg
     5101 ggttctccag ttcacacatt gacggatat
//
LOCUS       DAOTAC010000188         5118 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_13580_length_5118_cov_4.736520, whole genome shotgun sequence.
ACCESSION   DAOTAC010000188 DAOTAC010000000
VERSION     DAOTAC010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5118
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13580_length_5118_cov_4.736520"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="PLM74_08380"
     CDS             complement(<1..366)
                     /locus_tag="PLM74_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43128.1"
                     /translation="MPAIRRWSANDDGESGITQVELLISVVLAAVLVGAFFAYVQVGM
                     SANARVTAQAYYAQRLALAANQLLDGSGNAFLGMRSASSVQSRSGGGFEFRYSGTSHT
                     ESYWISGGELLRSADGGSAE"
     gene            complement(335..706)
                     /locus_tag="PLM74_08385"
     CDS             complement(335..706)
                     /locus_tag="PLM74_08385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HON43129.1"
                     /translation="MFPITRTRDDGFTLVEIMVVVVLLGLIGTFVYSGISTAMGTSQR
                     GKDKVEAVSVLQAVTEELTSSNLKSDIDKGSIAYDRDWIPNRYSVRYSVADTEYGVYR
                     LKVEVNQGAKVLASNTTLVCK"
     gene            complement(824..1828)
                     /locus_tag="PLM74_08390"
     CDS             complement(824..1828)
                     /locus_tag="PLM74_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43130.1"
                     /translation="MNQLARIHSSESGSVLLSVLVAVVVITVLAFGLLTNSTAAYRMQ
                     TSNARRVQAVLLAEGGAEAAYNRLKTDPSYRKDRGFTAAVGSEAVTGTISVDGSSYMI
                     VSTAAVGSQSQSVSLKVNTIAPPPPQIYGYGLYAAESVTGFNEETNIVVGSDIVSGGK
                     ITGFRTSEGFSKRENTPMPFPVLDPEEYRGYPAPDFYPGDDSRIVVNGYRFFDGTLGN
                     LSKNSVRIESPSDDGVLFVNGDLLAKNNLEFYGKITVIVAGDMECKNCLGGLEKLHLV
                     IGGDLELKNNMTLSGTVTVGGSFKAKNNTTVTFRAVGDSGVPGYDPTEIAGWSREWVR
                     "
     gene            complement(1836..2321)
                     /locus_tag="PLM74_08395"
     CDS             complement(1836..2321)
                     /locus_tag="PLM74_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HON43131.1"
                     /translation="MLRVEHSLQNEKGLTLVELLIVIVLAGLIIGVTWTVMTGAWSAW
                     ARTSTDASLDMQSEAGLMAVSSKMRIASSVDAADPTSPVIKVKGLSGDTVYSFRVDGT
                     DLKMATTVPGGTSTSATVARDVASFQVNPTEYAGSYTISITCTRSGMSETASTQVTIR
                     R"
     gene            complement(2290..2697)
                     /locus_tag="PLM74_08400"
     CDS             complement(2290..2697)
                     /locus_tag="PLM74_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HON43132.1"
                     /translation="MAKCMTEQSGYSLVEVLVALVFIGVAIAALFTAIMQGVYVQKAA
                     EMEADASDLANQAVEKYRSYFNNGNYDYLVTRSAEEEEAELAALGRSQFRRTIAVTQS
                     GTAPDRTWTIAATVTYDLKGKSRNVTCRTLFAE"
     gene            complement(2743..4200)
                     /locus_tag="PLM74_08405"
     CDS             complement(2743..4200)
                     /locus_tag="PLM74_08405"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011929062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HON43133.1"
                     /translation="MSSVWKGPLERIDEFRWRIPKAYKQGMRADAVVITDSEGLEVAR
                     DSEALEQLANSACLPGISGPALAMPDIHSGYGFPIGGVGAMRLEDGVITPGGIGFDIN
                     CGVRLVRTNLELGDATPKIKSWIDRLYRDVPSGLGTRGPVQLSANEVRQVLEHGAQWA
                     VRSGYGNQADLECTEDGGRMEAADSAAVSTRAMERGMPQLGTMGSGNHFVEIQVVEEV
                     IAPDVAKVFELHQNQVVIMVHTGSRGLGHQVCTDYLVTMNAALAKYGITVPDRQLACA
                     PFESSEGQRYFVAMACAANFAWANRQCITHWVRQATAREFGSTPESLGMTLLYDVCHN
                     IAKVEQHSVDGRLRKLCVHRKGATRAFPPGHPDVPAAYRGVGQPVIVPGDMGRNSYVL
                     VGTQKAMEVSFGSTCHGAGRLMSRNEALKAQRPSEVRQSLAEAGVYARSASKSALAEE
                     APEAYKDVNDIVDITHKAGISMKVVRLRPLGVVKG"
     gene            complement(4216..4722)
                     /locus_tag="PLM74_08410"
     CDS             complement(4216..4722)
                     /locus_tag="PLM74_08410"
                     /inference="COORDINATES: protein motif:HMM:NF014058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HON43134.1"
                     /translation="MADVKTIIEPFDEAGDAAFRVTASSLPEVFTGCGLALSNLILPW
                     AELGGEWEHRLGMPATRRIELAAPDLESLLVDFANEIIFLFDCEAFVPCGFEFRELGR
                     TEPAEGAGAACLDAEVAGIVLTENALLLAADQCGILGLVPKAASYHMIEVAELADGKW
                     TARLVLDA"
     gene            complement(4765..>5118)
                     /locus_tag="PLM74_08415"
     CDS             complement(4765..>5118)
                     /locus_tag="PLM74_08415"
                     /inference="COORDINATES: protein motif:HMM:NF015922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HON43135.1"
                     /translation="VGMDIARDNADVLVITNNGFGKRTSVSEYRPQRRGGKGLKACKL
                     TDKTGHIAGVKLVREENQLMLMTMAGYVIRFQISDVPRMGRYAQGVKAMALEEGDQVI
                     SIARVSSKIAEDLED"
BASE COUNT         1046 a         1638 c         1294 g         1140 t
ORIGIN      
        1 ctcggcacta cctccgtcgg ctgagcggag caattcaccc ccgcttatcc agtacgattc
       61 agtgtgagac gtgcccgagt atcggaattc gaatccccct ccagaacggc tttggacaga
      121 cgacgcgctg cgcataccca agaacgcgtt cccggagcca tccagtagct gattcgctgc
      181 cagggccagt ctttgagcat agtaggcttg cgcggtcact cgagcatttg cagacattcc
      241 cacttgtacg tacgcgaaaa atgccccaac cagcacggct gcgagcacga cactgattag
      301 caactctact tgagtgattc cgctctcgcc atcatcattt gcagaccagc gtcgtattgc
      361 tggcaagcac cttcgcaccc tgattcacct cgaccttgag gcggtacact ccgtactcag
      421 tatcagccac actgtacctt acagaatacc gattgggaat ccagtctctg tcgtaagcga
      481 tgctgccctt gtctatatcg gatttgagat ttgacgaggt caactcctcg gttactgcct
      541 gaagaactga aacagcctct actttgtctt tcccgcgctg gctcgtcccc attgccgtac
      601 tgattcctga gtagacgaag gttccgatta gcccaagtag aaccacgacc accatgatct
      661 ccacaagggt aaaaccgtca tctctagtgc gggtaatcgg gaacaccccc ctggcgagag
      721 atggttacaa tagtagcaac acgaaattgc tgtaccaatt atcacttatc agggatagat
      781 gtgcaactcc cgaagaaaag atagatggtg attcgggaaa atgctaccta acccactccc
      841 gcgaccagcc ggcaatctcc gtggggtcgt atccgggaac tcctgagtcc cctacagcgc
      901 gaaacgtgac cgtggtgttg tttttggctt tgaacgaccc tcctactgtg actgtgcctg
      961 atagcgtcat gttgttcttg agctcaaggt ctcccccgat taccaggtgc agtttctcga
     1021 gtcccccaag acagtttttg cattccatgt cgccggctac gatcaccgtg atcttcccgt
     1081 aaaactcaag gttgttcttg gccagcagat cgccgtttac gaagagcact ccatcatctg
     1141 atgggctttc gatgcggaca ctgttcttgc tgagattccc gagtgtgccg tcgaagaatc
     1201 tgtacccatt cacaacgatt cggctatcgt cacctgggta gaagtctggc gctggatagc
     1261 ccctgtactc ctcaggatcc aggaccggaa aaggcatcgg cgtattctcc cgtttgctga
     1321 agccttccga agtgcggaac ccggttatct tgccgcccga cacgatgtct gaacctacaa
     1381 cgatgttggt ctcctcgtta aatcccgtga cagactccgc ggcgtacagt ccatacccat
     1441 aaatctgggg cggaggcgga gctatggtat tcaccttcag cgatacgctc tgcgactgac
     1501 tacccactgc cgcagttgac acaatcatgt acgaagaacc gtcaaccgat atcgttcctg
     1561 tgactgcctc tgatccaacg gccgccgtaa aacccctgtc ctttcggtag ctgggatcgg
     1621 tcttcaagcg gttgtaggct gcttcggcgc ctccttccgc cagtaggacc gcctgtaccc
     1681 gtctcgcatt cgaagtctgc atgcggtatg ccgctgtgga gttggtcagc aacccaaaag
     1741 ccaacactgt tatgacgacc acggccacca gaaccgaaag caatactgag cctgactcag
     1801 acgaatgaat gcgcgccaat tggttcaccc cgctttcacc ttctgatggt gacctgtgtt
     1861 gatgcggtct ctgacatacc ggaacgtgtg catgtaatcg atattgtgta tgaaccggcg
     1921 tactctgtcg gattcacctg gaaagatgcc acgtctcttg cgaccgttgc actggtagat
     1981 gtaccgcccg gcacagtcgt agccatcttc agatcggtac catcgactct gaaagagtag
     2041 acagtatcgc ccgaaagacc ctttaccttg atcacaggac ttgtgggatc ggccgcgtca
     2101 acagacgacg ctattctcat tttggacgaa acagccatca gccccgcttc agactgcata
     2161 tcgagagagg catcggtcga tgtacgtgcc catgcactcc acgctccggt catgactgtc
     2221 caggtcactc ctatgatgag ccctgccagc acaatgacaa tgagcagctc gaccagggtt
     2281 agccctttct cattctgcaa agagtgttcg acacgtaaca ttccgagact ttcccttcag
     2341 gtcatatgtg acagtagccg cgatggtcca ggtgcggtca ggcgccgtgc cggactgcgt
     2401 gactgcgatc gtcctgcgaa actgcgatct gccgagcgct gctagctcag cctcttcctc
     2461 ctcggcactc ctcgtaacca ggtagtcgta gtttccattg ttgaagtacg agcggtactt
     2521 ctcgaccgcc tggttagcca gatccgatgc gtccgcttcc atctccgcgg ctttctgcac
     2581 atatactccc tgcataatcg cagtgaagag ggccgcgatc gcaacgccta tgaacactag
     2641 ggctacgagc acctcaacta gagaataccc cgattgttca gtcatgcact tcgccaatac
     2701 cccacccccc cggagcgtgg tcgcgccgca agatacgtca ttctatccct tgactacccc
     2761 caacggccta agccgcacga ccttcataga tatccccgcc ttgtgagtaa tatcgacaat
     2821 gtcgtttaca tccttatagg cctcgggcgc ctcctcagct agtgcgctct tgctggccga
     2881 gcgggcatac actcccgcct ctgcaagcga ctgcctgacc tcgctgggcc tttgggcctt
     2941 cagagcctcg ttgcggctca tcagacggcc tgctccgtga catgtggagc cgaacgagac
     3001 ctccatcgcc ttctgcgtgc ccacgagaac ataggagttt cggcccatgt cgcctggaac
     3061 gatcaccggc tggccgaccc ctcgatacgc tgccggcacg tccggatgcc cgggcgggaa
     3121 ggcgcgcgta gctccttttc tgtggacaca gagctttcgg agacggccat ccacagagtg
     3181 ctgttcaacc ttggcgatgt tatggcacac atcgtatagc agcgtcatgc ccagactttc
     3241 aggagtcgac ccgaactcgc gcgcggtagc ctgcctgacc cagtgagtga tacactggcg
     3301 attcgcccag gcaaagttgg ccgcgcatgc catggctacg aaatacctct gcccctcgga
     3361 tgactcgaag ggggcgcacg cgagctgcct gtcgggaacg gttatgccgt attttgcgag
     3421 ggctgcgttc atcgtgacca gatagtccgt gcaaacctgg tgccccagcc cacgcgatcc
     3481 tgtatgtacc attatcacga cttgattctg atgtagttcg aatactttcg ccacatccgg
     3541 cgcaatcacc tcttccacga cctgtatctc tacgaagtga ttgccggacc ccatagtgcc
     3601 aagttgcggc atcccgcgct ccatggcccg ggtgctcacg gctgccgagt ccgccgcctc
     3661 catcctgccc ccgtcctcgg tgcactcaag gtcagcctgg tttccgtagc ccgatcgcac
     3721 tgcccattgg gccccgtgct caaggacttg ccgcacttca tttgcgctca gctgaacggg
     3781 tccccgcgtg ccaagccccg acggcacatc gcggtacagc cggtctatcc atgacttgat
     3841 cttgggagtc gcatcgccaa gctccagatt cgttctgacc agcctgactc cacagtttat
     3901 gtcgaagccg atgcctccgg gggtgatcac cccatcttcc aggcgcatcg cccccacgcc
     3961 cccgatggga aagccgtatc ccgaatggat atccggcatg gccagggcgg gcccgcttat
     4021 gcctggaagg caggccgagt tggccagctg ctccagagcc tcgctatcgc gggcaacctc
     4081 cagcccctcg ctgtcagtga tcacgacggc gtctgcgcgc atgccctgct tgtaggcttt
     4141 gggtatgcgc caccggaatt cgtcaattcg ctctagcggg cctttccaca cactcgacac
     4201 gtgctcaacc tcctctcaag catccagaac cagccgagcc gtccacttac cgtcggctag
     4261 ttccgctact tctatcatgt gataagaagc cgccttgggc acaagcccca gtatcccaca
     4321 ttgatcggcc gccaaaagga gggcgttctc cgtcagcaca atgccggcga cttccgcatc
     4381 gagacacgcg gcgcccgcgc cctcggccgg ctccgtccgg ccgagctcgc ggaattcgaa
     4441 tccgcaaggc acaaatgcct cacagtcgaa aagaaagatg atctcgttgg cgaagtccac
     4501 cagcagcgac tcaagatcgg gcgcggccag ctcaattctt cgagtagcgg gcatgcccag
     4561 tctatgctcc cactcgcccc caagctctgc ccacggaagt atcagattgg acagagcgag
     4621 cccgcagccg gtgaatacct ccggaagcga gctcgctgtc actcggaagg cggcgtcacc
     4681 ggcctcatcg aaaggttcaa tgatggtctt gacgtcggcc attgatttgt ctcctcttgg
     4741 cgctccccgc caggtcagtc gctactagtc ttccagatcc tccgcaatct tggacgacac
     4801 ccttgcgatt gatatcacct gatcgccctc ttcaagagcc attgccttga ctccctgcgc
     4861 gtatctgccc attctaggca cgtcggagat ctggaaacgt atcacatacc ctgccatcgt
     4921 catcagcatc agctggttct cctcgcgcac cagcttgacc ccggcgatat ggccggtctt
     4981 gtcagtcagt ttgcaggcct tgagcccctt gccgccgcgc cgctgcggac ggtattccga
     5041 cactgaggtg cgcttgccga atccgttgtt cgtgatcacc agcacatccg cattgtcgcg
     5101 cgcgatgtcc atgcccac
//
LOCUS       DAOTAC010000189         5038 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_13860_length_5038_cov_4.548063, whole genome shotgun sequence.
ACCESSION   DAOTAC010000189 DAOTAC010000000
VERSION     DAOTAC010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5038
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13860_length_5038_cov_4.548063"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="PLM74_08420"
     CDS             complement(<1..698)
                     /locus_tag="PLM74_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HON43136.1"
                     /translation="MRHTTMRVLMVGAGIATGFLVHRAFPVAVMMPESQGAGLRVMLG
                     AMTGGGLIGFAITPLLHQMASRFGKYVDSGVRNISASGIIAGAVGMIVGLIIANLLAI
                     PIALIPSLGSYVGIALNVFFGLAGMVIGSHKREEIMSILAPLPRSGELVRSAVARDRS
                     MRSYAGKAKPKILDTSVIIDGRIADIADSGFVEGTLVVPGFVLEELQHIADSSDVLKR
                     NRGRRGLDILNRMQ"
     gene            complement(796..1278)
                     /locus_tag="PLM74_08425"
     CDS             complement(796..1278)
                     /locus_tag="PLM74_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HON43137.1"
                     /translation="MFSVGDRVVYPMHGAGVVEAIEEREVLGLRDNYYIMRLQVGDMK
                     VMVPRSSASEVGLRPVIGPEDIPRVYEVLSGEKTAMSQNWNRRYRANMEKMKTGDIFS
                     VAEVVRNLGIRDREKGLSTGERRMLENAKQILTSEMAMVEKRSIDDISRTIDSILDSA
                     "
     gene            1528..1923
                     /locus_tag="PLM74_08430"
     CDS             1528..1923
                     /locus_tag="PLM74_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HON43138.1"
                     /translation="MADLSCNDFQDSVCEYLIRHRSVLDVMSKFQEASARVNRAMAKA
                     VTTCGCLEIQAHRQKFPEDVSLEDLRRLVCSHLSGELCPECREALETELGQTIFYVAA
                     LCAITGLDMNEIMAKEYDRLRMLGLFNLT"
     gene            complement(1962..3020)
                     /gene="disA"
                     /locus_tag="PLM74_08435"
     CDS             complement(1962..3020)
                     /gene="disA"
                     /locus_tag="PLM74_08435"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018087016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HON43139.1"
                     /translation="MSEKLRKIFGMVAPGTPLYDGLENILRARTGGLIVVGDTPEVMG
                     IVKGGFRLDAEFSPAALYELAKMDGAIILSSNADRILHANVTLAPDPDIPSRETGLRH
                     STAERVAKMTGQLVIAISQRRSVISVYWGHTRYVLRDIGVVLAKANQALQTMANYRDA
                     LVKALANLTALEFIEEATLVDVCRVLQRYELMRRAWDEVTDYIAELGSEGRLVKIQAD
                     EVVGGMWEDVLFVAMDYIPDGGQAEAERLMRGVAEWPFDEVLELTSLSRALTQSSGAS
                     LESAMSPRGYRALSKISRLPISVIQNVVEGFGSLKAIRSATVEELDEIEGIGDVRARA
                     VREGLARLRDQVVRNLVM"
     gene            complement(3020..4423)
                     /gene="radA"
                     /locus_tag="PLM74_08440"
     CDS             complement(3020..4423)
                     /gene="radA"
                     /locus_tag="PLM74_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HON43140.1"
                     /translation="MKGPKGVFVCQECGYESAKWMGKCPACASWNRFVEEVWVEEAPA
                     RTSSRRSGSGRGLASAAARPIPVGEVISGDEERLITGIGEFDRVLGGGIVAGSVALIG
                     GDPGIGKSTILLQVSASVARRMGPVLYVTGEESARQVGLRAARLDAKCERLLVLPETD
                     VISIIDQIHAVHPALVVIDSIQTMDYAGLESSPGSVGQVRECTALLMRAAKEQGVPIF
                     LVGHVTKSGAIAGPKVLEHIVDTVLYFEGESSHAHRIIRAVKNRYGSTNEVGIFAMTD
                     AGLEQVENPSEMLLSERPGGAAGSVVVASLEGTRPLLVEVQALVARSVTGTPRRTTTG
                     VDYNRTAIIIAVLERRAGFAIGVEDIYVNVAGGVALNEPAVDLGIAAAVVSSFRGVPA
                     SGGTVVFGEVGLAGELRAVNHADVRVAEAQRLGFGRCVVPRHNLKSMTRRPKDVEIIG
                     AASVREALDAVFAGVKA"
     gene            complement(4459..>5038)
                     /locus_tag="PLM74_08445"
     CDS             complement(4459..>5038)
                     /locus_tag="PLM74_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016742864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HON43141.1"
                     /translation="LLQVLEDGRLTDGQGRTVDFRNTVLIMTSNVGATQIQRDTVLGF
                     RTERDERDSYERMRDKVMSELKKTFRPEFLNRVDEVIVFHALGRAELEQIVELMLAEV
                     RSHLEPRGIHLSFTPAAKDALVNEGYSPEYGARPLRRVIQRLVEDRLSDEIIAGRIKD
                     GQEVRVDAQEGTLVFRVQNQKGSEAGVTKDIA"
BASE COUNT         1002 a         1672 c         1328 g         1036 t
ORIGIN      
        1 ttctgcatcc tgttcaggat gtcgagaccc ctgcgtcccc tattccgctt aagaacgtcg
       61 gaagaatcag ctatgtgctg aagctcctcc agcacaaagc ctggaacgac aagtgttccc
      121 tcaacaaacc cgctgtcggc aatatcggca atgcggccat cgatgatgac gcttgtatca
      181 agtatcttcg gcttggcctt gccagcatag ctcctcatac tgcggtcgcg cgcaacggcc
      241 gaccgcacca gttcgccgct gcgcggaagc ggcgccagta tggacatgat ttcctcccgc
      301 ttgtgcgagc caatgaccat tccggccagg ccgaaaaaga cgttcagggc tatcccgacg
      361 tagctgccca gcgacggtat gagagctata ggaatggcga gtaagttagc tattattagg
      421 ccgactatca taccgacagc gccggcgatt atcccgctcg ccgagatgtt ccggactccc
      481 gaatccacat acttgccaaa ccgcgaggcc atctggtgca gaagcggcgt gatggcgaac
      541 cctatcagcc ctccgccggt catcgcaccc aacataactc tgagccccgc gccctggctc
      601 tccggcatca tgacggccac cggaaacgcc cgatgaacaa gaaaacctgt ggcgatcccg
      661 gccccgacca tcaggactcg catagtcgta tgacgcactg agcgcttcac ctccctccag
      721 ccggcagcag atgaacccgg ctgttccagg ctagtatatc cagtggatgg tcaagtatga
      781 aaatgggcgg cccctctagg ccgagtctag tatggagtcg attgttcgag atatatcgtc
      841 gatggagcgc ttctccacca tggccatctc gcttgtcagt atctgcttgg cgttctcgag
      901 catgcggcgt tcaccagtgg aaagtccctt ttccctatcc ctgatgccca gattgcgcac
      961 tacctcggcc acggagaaga tgtcgcccgt cttcattttc tccatgttcg cccggtatct
     1021 gcgattccag ttctggctca tcgcggtctt ctccccgctg agcacctcgt acaccctggg
     1081 aatatcctca ggcccaatga ccggcctcag gcccacttcc gaagcgcttg atcggggaac
     1141 catcactttc atatcgccga cttgtagacg catgatgtag tagttgtccc gcagcccgag
     1201 gacttctcgc tcttcgatgg cctcaaccac cccagctcca tgcatggggt agaccaccct
     1261 gtctcccacg ctaaacatgg tttgcatcac ttcctcctcg agcgcctttt gtaattgtac
     1321 cattggctgc caggccagtc aattcgcgcg agcgacgcat cgacccaccg accctgaccc
     1381 ggggctgatc cggagtcgct aatccggggt tgttgattcg gctctgattc ggctctgatt
     1441 gacaacgcgg tggaccaaac gtataatcaa gttgaacttc agcccggcca tatcacctgc
     1501 gtgtaccgga tttggaggtg agcgctcttg gccgacctct catgtaacga cttccaggat
     1561 tctgtctgcg agtacctaat tcgccaccga agcgttctcg atgtaatgtc gaaattccaa
     1621 gaagccagcg cgcgagtcaa ccgtgcgatg gccaaagcgg ttacgacctg tgggtgcctg
     1681 gagatccagg cccaccggca gaagttcccc gaagatgtgt cgctggaaga tctccggcgt
     1741 ctagtctgtt cacacctcag cggcgagctg tgccccgagt gcagagaggc tctcgagacc
     1801 gaattggggc agacgatttt ctatgtagcg gccttgtgtg caataacggg cttggacatg
     1861 aacgagatta tggcgaagga atacgatcgc ctgcgcatgt tgggcttgtt caacctcacc
     1921 tgagtagctc cggccgccaa tgtgtgatat tgagtcctcg atcacatgac aaggttgcga
     1981 acgacttggt cgcgaaggcg agccagccct tcgcgcactg ccctcgctcg cacatctcca
     2041 atgccttcta tctcgtcgag ctcctccacg gtagcgctgc gtatggcctt gagtgatccg
     2101 aatccctcca cgacattctg gatcaccgag attggcaacc gcgagatctt cgacagagcg
     2161 cggtagcctc tgggcgacat agctgattcc aacgaagctc ccgagctctg ggtcagcgcc
     2221 cgggataggc tcgtcagctc cagcacctcg tcgaagggcc actcggccac tccccgcatc
     2281 aatcgctccg cctcggcctg gccgccatcg ggtatgtagt ccatggccac gaagagcacg
     2341 tcctcccaca tgcctcccac gacctcgtcg gcctggatct tcaccaggcg accctccgac
     2401 cccagttctg ctatgtagtc ggtaacctcg tcccatgcac gtctcatcag ttcgtatctc
     2461 tgcaaaaccc gacatacgtc taccagcgta gcctcttcga tgaactccag cgcagtgagg
     2521 ttggcaagcg ccttgaccag tgcgtcgcgg tagttggcca tggtctgcaa tgcctggtta
     2581 gctttggcca gcaccactcc tatatcgcgc aggacgtatc tggtatgccc ccagtacacg
     2641 ctgatcaccg agcgcctctg agagatcgcg ataaccagtt ggcctgtcat cttggcaaca
     2701 cgctcggcgg tgctgtgacg caggccggtc tcgcgtgacg gtatgtcagg gtcgggcgca
     2761 agagtgacat ttgcgtgaag tattctatca gcattggacg ataggattat ggccccatcc
     2821 atcttggcaa gctcgtaaag ggcggccggc gaaaactcgg cgtccagcct gaacccgcct
     2881 ttcacgatcc ccattacctc aggagtgtct cccacaacta tgagcccgcc ggtccgggca
     2941 cgcaagatgt tctccagccc atcatagaga ggagtgccgg gcgcgaccat gccgaaaatc
     3001 ttgcgcagct tctccgacat caagctttca cccccgcaaa caccgcgtcc agggcttcac
     3061 gcacgctcgc agcccctatg atctcaacat ccttcggccg cctggtcatg ctcttaagat
     3121 tgtgacgcgg aaccacacac ctgccaaatc cgagacgctg ggcttcggct actctcacat
     3181 cggcgtgatt caccgccctg agctctccgg ccaaccctac ctcgccgaag accaccgtgc
     3241 cgcctgaggc gggcacgccg cggaaactgg ataccacggc cgctgctatt cccaaatcaa
     3301 cagccggttc gttaagggcg actccacctg ccacgttcac gtagatgtct tccacgccga
     3361 tggcaaagcc tgctcgcctt tcgagcaccg caatgattat ggccgtcctg ttgtagtcga
     3421 cgcccgtagt cgtcctgcgt ggagtcccgg taaccgaccg agcaaccagc gcctgaacct
     3481 ctaccaggag cggcctggtc ccttccaggc tggccacgac tacagaaccg gccgcgccgc
     3541 cgggtcgctc cgacaaaagc atctccgagg ggttctctac ctgctcaagc ccggcatcgg
     3601 tcatagcgaa gatcccaact tcgttggtcg agccatacct gttcttcaca gcccggataa
     3661 tgcgatgggc gtgtgagctc tcaccttcga aatagagcac agtatcgaca atgtgctcca
     3721 gaaccttcgg gccggcaatc gcgccggatt tggtgacatg ccccacaagg aagatcggca
     3781 ctccctgttc cttggcggct cgcatcagca aggccgtgca ctcacgcacc tgccccacgc
     3841 tgcccggcga cgattccaga ccggcgtagt ccatcgtctg gatcgaatcg atcaccacca
     3901 acgccggatg gaccgcatgg atctgatcga taatggagat tacgtccgtc tccggaagga
     3961 ccagaaggcg ctcgcacttg gcatcgagcc gcgccgcgcg caagccgacc tgccgggctg
     4021 actcctcgcc cgtgacgtag agcaccgggc ccatccgccg cgcaacactc gccgacacct
     4081 gcagcagtat ggtggacttg cctatgccgg ggtcgcctcc tatcagcgct accgaccccg
     4141 ccactatccc gccgcccagc actcggtcga actcgcctat cccggtgatg aggcgttctt
     4201 cgtccccgga gatcacctca cccaccggga tcggcctcgc cgccgcagag gccaggccgc
     4261 gcccggagcc gctgcgccgg ctggacgtgc gcgccggcgc ctcctccacc cagacttctt
     4321 cgacgaaccg gttccagctg gcacaggccg gacacttgcc catccatttg gcagattcgt
     4381 atccacattc ctggcagacg aaaacgccct tcgggccctt catgcacgac cgcccccaga
     4441 gttctggctc tcacgctcct aagctatatc cttcgttaca ccagcctctg aacccttctg
     4501 gttttgaacg cgaaagacga gagtgccctc ttgtgcgtca acgcgcactt cctggccgtc
     4561 cttgatgcgg ccggcgatga tctcgtccga cagacgatcc tccacaagcc gctgaatcac
     4621 ccgcctgagc ggccgtgctc cgtactcagg gctgtaacct tcgttcacaa gagcgtcttt
     4681 ggcagcaggc gtgaacgaaa ggtgtatgcc cctgggttcg agatgagagc gaacctcggc
     4741 aagcatgagt tcgacaatct gctcgagttc cgcgcgcccc agtgcatgaa agacgatcac
     4801 ctcatccaca cggttcagga actccggcct gaaggtcttc ttcagctcag acatgacctt
     4861 gtcgcgcatg cgctcgtacg agtcgcgttc gtcgcgctca gtgcggaagc ccagaactgt
     4921 gtcgcgctga atctgggtgg ctcctacgtt tgacgtcatt atcagcacgg tgttccgaaa
     4981 gtccacagtc ctgccctgac cgtcggtaag gcgcccgtcc tccaagacct gcaggagg
//
LOCUS       DAOTAC010000190         5026 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_13898_length_5026_cov_4.405150, whole genome shotgun sequence.
ACCESSION   DAOTAC010000190 DAOTAC010000000
VERSION     DAOTAC010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5026
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13898_length_5026_cov_4.405150"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="PLM74_08450"
     CDS             <1..407
                     /locus_tag="PLM74_08450"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HON43142.1"
                     /translation="GYPAPRINCKTISGERLCLADCAGDLVLLTFFSTASGASLAHVS
                     VLAQMRAGGLEGTRIVAIAVAEKADAVLRYVEARKLDLPVIADPKGELARLYDITAVP
                     TTFVIDAEGIVRDLRVGAMTRAQLERAVRSAL"
     gene            complement(1040..1966)
                     /locus_tag="PLM74_08455"
     CDS             complement(1040..1966)
                     /locus_tag="PLM74_08455"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HON43143.1"
                     /translation="MDSVTVFEALRGIATPGLDRVIKVVTDLGSTTFYMIVIPILYWC
                     ISKSLGFGLAIVLSVSNYINVGIKFIVCRARPYVAFPHLDAPEYLKLTGTGYSFPSGH
                     AQVTSTFWTYLARSVRRNWVTVVGIAVVVVVAFTRVYATVHYPTDVLVGAPLGLAIAL
                     AYSEIERRLRASGTEGTSRVGLQLAVSIVLPGLAFALSAPRNPAFTEEVAQVLGFLAG
                     AGIGYTLENAYVKYDVRAGLPVQVIKVILGMAGLMGVRYGLKAVFPQAALWNMLRYAA
                     VAIWATLGAPFVFKALFGRRAAAQAERHRPGH"
     gene            2046..3074
                     /locus_tag="PLM74_08460"
     CDS             2046..3074
                     /locus_tag="PLM74_08460"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HON43144.1"
                     /translation="MIVSLGDLMVDAYFQIPDRRNPRTDTQGRVELAAGGSAANFAVW
                     IARHGRKSGLIGRVGHDFLGRALVADLEHEGVCPYLVFDPDPDYGTGRVGVIVAADGE
                     RDMVCDRRANARLSVEDIPEHVVAGAEWLHVSGYVFLEEAPAKAARHSIDIACEAGVP
                     VSVDPAAYSFIHALGRQAFLKLCDGATVILPNLDEGRAMTGLEQPEDIASCLLDHFPV
                     VALKLGADGCLGMARQWAGMAGITGAAGSMGAGAGVAAGAGAAGRSDAAGRAGGVDAG
                     GHVLEKGLVAAVGFAAVPTRVVDTTGAGDAFDAGFVLEYSRGAGLAASLARGNEMGAR
                     VVSRMGAR"
     gene            complement(3110..3562)
                     /locus_tag="PLM74_08465"
     CDS             complement(3110..3562)
                     /locus_tag="PLM74_08465"
                     /inference="COORDINATES: protein motif:HMM:NF016535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HON43145.1"
                     /translation="MRFLNAGALWAVAAAAASGIAMAFQGAVNATAAKAIGLWETTFV
                     VLASGTALTGAGLALGLSQGGLARLGTAPWWSLLGGPVGPVITAAVAYGIRKAGAVGA
                     TTAIIVGQISMAALIDHLGWLGVERIPFSPLKFLGIALLAVGAWILLN"
     gene            3802..>5026
                     /locus_tag="PLM74_08470"
     CDS             3802..>5026
                     /locus_tag="PLM74_08470"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HON43146.1"
                     /translation="MRRLMSILACFVVLLMITTAGFAAETLAKKQELSFNVGTEPETL
                     DPAKSTGVPEAIIELNCFEGLTRMDENNIPQPAIAESWTVSKDGKTYVFKLRKTKWSN
                     GDPLTAHDFEWSWKRALAPETASEYATQLWYLKNGQAYNEGTLKDASKVGVKALDDYT
                     LEVVLENPTTYFLQLCNFPTLMPVNRKIVEKNPEGWFQDIKNYIGNGPFKIVKWEHNA
                     VMELVPNENYWRRDKVKLEKLVLYLVDNPQTALSMFETGQLDWMDAPPLAQIPRLEKE
                     GWLFRTPYIGTYYYMFNVTKKPLDDVRVRKALAMALDRQMLIDRVIRGGQKPAFAYVP
                     FGLADGKPGADFRTVAGNDYIKENLAVAKKLLADAGYPNGKGFPTLTILYNTSENHKK
                     IAEAIQEMWKKNLGIN"
BASE COUNT         1037 a         1449 c         1540 g         1000 t
ORIGIN      
        1 caggatatcc ggccccgaga attaactgca agactatcag cggggagcgg ttgtgcctgg
       61 cggattgtgc gggcgatttg gtgctgctga cgttcttcag caccgcatcg ggcgctagcc
      121 ttgcccatgt atcggtcttg gctcaaatgc gcgcaggagg actggagggc acgaggattg
      181 tggcgattgc ggtggccgag aaggcggacg ccgtactccg atacgtggaa gctcgtaagc
      241 tggacctgcc cgttatcgcc gatcccaagg gcgaacttgc gcgcctatat gacattactg
      301 ccgttccgac gacattcgtc attgatgcag agggcatagt gcgtgacctg cgcgtgggcg
      361 ccatgacccg cgcacaactg gagcgggcgg tgcgctcggc gctctagggc atccgcctgc
      421 gcctactggc aaccaatgtg gaaatgagca cttcggactc gatagtgggc cgtgtatatg
      481 tgccgtacgc tgcacgcttg ccgcgaaagg cccatctacc tggccttttg cgacctgttc
      541 atattatgaa caggttttcc ccggatgtgt cgcgagtccg gacgagtact gatgtatatc
      601 ggaacgtata tccgcataaa ggctggcgac gggatgatcc agtggctgcg gtcgtgaata
      661 gtggcatgat gtggctggcg aatggggatt attgctaatg tcgtccgcat atcgttagcc
      721 catttgggta tacatgttag gacctttccg tcttaggccg cctatgcatc tattcgttcc
      781 gttatgcatc acctcttgtc cgttttgtcg acatatcgtc gggaacgtgt tcataaaatc
      841 tacatctaga ttcttccata taccctgtgg tccgccgctg cgctctccct gctgctaggc
      901 ttcgcttcgt ctgtgatcga aaccgcatgt acctgtggat tacatatgtc tgcactcttc
      961 gggcctgatc acctcgttca catgagatgt ttcgcgtgga tgcacagaat ctgccgggcc
     1021 cccgcgtgat gaccaaccct caatgcccag gtcggtgcct ttctgcctgc gctgccgccc
     1081 gccgaccgaa caacgccttg aagacaaacg gcgctcccag cgtggcccag atggctactg
     1141 ctgcgtagcg aagcatattc cacagcgctg cctgtgggaa gactgccttc agtccgtacc
     1201 tgacccccat caacccagcc atgcccagga tcaccttgat gacttgaaca ggtaggccgg
     1261 cgcgaacatc gtacttgacg tacgcattct cgagggtgta cccgattccc gcccctgcta
     1321 ggaagcccag cacctgggct acttcctcgg tgaacgcagg attacggggc gcggacagcg
     1381 cgaaggcgag ccccggcagt acgatggaaa cagctagttg gaggcccact ctgctcgtgc
     1441 cttccgtgcc cgatgcgcgc aggcgtcgtt cgatttctga gtatgcgaga gcgatggcca
     1501 ggcccagcgg cgctccgacc agaacatcgg taggatagtg gaccgtggca tagactcgcg
     1561 tgaaagccac taccacaacc actgcgattc cgacgacggt tacccaattc cgccggaccg
     1621 agcgggccag gtaggtccag aatgtggacg taacttgagc gtgcccgctg gggaatgagt
     1681 atcccgtccc ggtcagtttc aggtactcgg gcgcgtcaag gtggggaaaa gcgacgtaag
     1741 gcctcgcccg gcacacgatg aacttgatcc ccacgttgat gtagttcgaa accgacagga
     1801 ctatcgctag accaaacccc agtgacttgc tgatgcacca gtagagtatt ggtatcacaa
     1861 tcatgtagaa tgttgtcgag cccagatcgg ttacgacttt gatcaccctg tcaagcccgg
     1921 gggttgcgat gcccctcaag gcctcgaaca ctgtgacact gtccaatgcc gatcacctcc
     1981 gggagatgac gttcttcgca taggtctaga atcctttgtg ttcttgtaaa ggagcctgag
     2041 cccctatgat tgtgagcctt ggagacctga tggtcgacgc gtactttcaa atacccgaca
     2101 gacgaaaccc acgcaccgac actcaaggac gagtggagtt ggccgcaggc gggtcggctg
     2161 ccaatttcgc tgtgtggatc gcgcgccacg gccgcaagtc aggcctgata gggcgggtcg
     2221 gccacgactt cttggggcgg gcgttggtcg ccgacttgga gcatgaaggt gtgtgcccgt
     2281 atcttgtatt cgatcccgac ccggattacg gcaccggtcg ggtgggggtg attgttgcag
     2341 ccgacggcga gcgcgacatg gtatgcgacc ggcgggccaa tgcgcgcctt tcggtggagg
     2401 acattccaga acatgtcgtg gccggcgccg aatggctgca cgtttcggga tacgtattcc
     2461 tggaggaggc gccggccaag gccgcgcgac actccattga catagcctgc gaagccggag
     2521 tccctgtgtc ggtagaccct gcagcgtact cattcatcca cgccctgggg agacaggcct
     2581 tcctgaagct gtgcgacggg gccacggtga tccttcctaa cctggacgaa gggcgtgcca
     2641 tgacaggact ggagcagcct gaagacatcg cgtcctgcct gctcgaccac tttccggtgg
     2701 tcgcgctgaa gctgggcgcc gatgggtgtc tgggcatggc gcggcaatgg gcggggatgg
     2761 cgggcatcac gggtgcggcg gggtcgatgg gcgcaggagc gggggtcgca gcaggagcag
     2821 gtgccgcagg cagatccgac gccgcgggca gagcaggcgg tgtagacgca ggggggcatg
     2881 tgctcgaaaa agggctcgtg gctgcggtag gattcgcggc tgtgcccact cgggtagtcg
     2941 acacgaccgg cgcaggggac gccttcgatg ccgggttcgt gcttgagtat tcgcgcgggg
     3001 cggggttggc cgccagtctg gcgcggggca acgagatggg agccagggta gtgtcgagga
     3061 tgggcgcacg gtgacatgcg cggcatacag gcgaagcgtg gcagtcgcat cagttgagca
     3121 atatccaggc cccgaccgcg agtagcgcta ttcccaggaa cttgagcgga ctgaacggga
     3181 tgcgctctac tcccagccag ccaaggtggt cgatgagtgc ggccatactg atttggccca
     3241 ctatgatggc ggttgttgcg cccactgcgc cggccttgcg tattccgtaa gccaccgcag
     3301 ctgtgatgac cggtcctaca ggcccgccga gcaggctcca ccatggggct gtgcccaggc
     3361 gtgccaggcc tccctggctt agccccagag ccagtccggc gcctgtgagt gcagtgccgg
     3421 aggccagcac tacgaaggta gtctcccaca ggccaatggc ctttgcggcc gttgcgttca
     3481 cagctccctg gaacgccatt gctatgccgc tggccgctgc agccgccaca gcccacaggg
     3541 cgcccgcgtt gaggaagcgc ataaacacca caacctttcc tgtttgatat ccgcacctag
     3601 tcccgttttg taatcattcc cacagacggg tccggaattg caggagacgc gtagcaggaa
     3661 tccgacatgg taaggagaat agaaatcata ctatggactc cccctggttc agatgagtcc
     3721 aaatccgagg ggaacaggta ttcgctcatc tgagcctgcg ggcaacgact gaccactaac
     3781 aacagaggga ggcaaggttg tatgcgaaga ctcatgtcaa tactcgcgtg cttcgtggtg
     3841 ctgctgatga tcacaacggc aggtttcgcc gcggagacac tcgccaagaa gcaggagctc
     3901 agcttcaacg tgggaactga gcccgagacg ctcgacccgg ccaagtccac cggagtaccc
     3961 gaggctatca tcgagctgaa ctgcttcgag ggccttacta gaatggacga gaacaacatt
     4021 cctcaaccgg ccatcgctga gagctggacc gttagcaagg atggcaagac ctacgtcttt
     4081 aagcttcgca agaccaagtg gtctaatggc gaccccctga ccgctcatga ttttgagtgg
     4141 tcttggaaga gggcgctggc tccggagact gcgtcggagt acgctacgca gctctggtac
     4201 ctcaagaacg ggcaggctta caacgagggc actctgaaag acgcttccaa ggtaggcgtc
     4261 aaggcactcg acgactatac cctcgaagtg gtgctggaga accccaccac ctacttcctg
     4321 cagctgtgca acttccccac actgatgcct gtgaaccgca agattgtcga gaagaaccct
     4381 gaaggctggt tccaggacat caagaactac atcggcaacg ggccgttcaa gatcgtcaag
     4441 tgggaacaca acgccgtcat ggagctagtg cccaatgaga actactggag gcgtgacaag
     4501 gttaagctgg agaagttggt gctttacctc gtcgataacc ctcagacggc gctatcgatg
     4561 ttcgagactg gccagctcga ctggatggac gcccccccgc tcgcgcagat cccgcggctc
     4621 gaaaaggaag gttggctgtt ccgcactccc tacattggca cgtactacta catgttcaac
     4681 gttaccaaga agcctcttga tgacgtcagg gtgcgcaagg ctctcgccat ggctctagac
     4741 cgccagatgc tcatcgaccg cgtgatcagg ggcggccaga agccggcctt cgcctatgtt
     4801 cctttcggct tggctgacgg caagccaggc gccgacttcc gcaccgtggc cgggaacgac
     4861 tacatcaagg agaatcttgc agtcgcgaag aagctgctgg ccgacgccgg ttatcctaat
     4921 ggcaagggct tcccgacact gacgattctc tacaacacta gcgagaacca taagaagatc
     4981 gccgaggcca tccaggagat gtggaagaag aacctgggca tcaacg
//
LOCUS       DAOTAC010000191         4964 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_14106_length_4964_cov_5.961907, whole genome shotgun sequence.
ACCESSION   DAOTAC010000191 DAOTAC010000000
VERSION     DAOTAC010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4964
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14106_length_4964_cov_5.961907"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="PLM74_08475"
     CDS             <1..217
                     /locus_tag="PLM74_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43147.1"
                     /translation="TRIHIMLPAYACGFVAGVTPKNRGAVAGANSDNSYLPNHPESEE
                     VDQKACRKTNRMSQNLVEPNAPVSGTR"
     gene            complement(864..1889)
                     /locus_tag="PLM74_08480"
     CDS             complement(864..1889)
                     /locus_tag="PLM74_08480"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HON43148.1"
                     /translation="MDAIWWITTHLAPERCTSDKFFYDAMESQSEYGLPVIYKAFDPG
                     KAWHWDDRGRILDYLFATHGQGARLLDFGPGDGWPSLPVAPFAGEVVGVDASARRVQV
                     CAGNAARMGINNARFVHIPAVGPLPFEDESFDGVMAATSIEQTPDPREALREIYRVLR
                     PGGRLRMDYEGLGCYRGGKEREVWLLDMGEACRLIIYDRHVEQERADEYGLDLSMAVG
                     EVASWLTGRAGATAASEGMAVGIEHVTPDALDALRPVITGAWVCTLSHPSGATYARWL
                     REIGFSVVRPTHNGGHFARKLFGSMLPEDRPTSLEGVTELLSPLVQVVVEMPAPIEMD
                     PMITAVK"
     gene            complement(2135..3304)
                     /gene="grdD"
                     /locus_tag="PLM74_08485"
     CDS             complement(2135..3304)
                     /gene="grdD"
                     /locus_tag="PLM74_08485"
                     /EC_number="1.21.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     component C subunit alpha"
                     /protein_id="HON43149.1"
                     /translation="MAAVAELFREMAEGLETGQFGRRPRVGLTVLGSEHGPDEMVHGA
                     ELAARAYSDVEVVLVGPVSPEDARGRGLDAVWASDEAGQHARMEELLDSGQLDACVTM
                     HYTFPIGVSTVGRVVTPGRGREMFIATTTGTSGTDRVENMVKNAVYGIAAAKACGVTE
                     PSVGVLNVDGARQVERALRKLDSNGYDVRFAESARADGGAVMRGNDLLVGVPDVMVCD
                     TLTGNLLMKVFSSFTTGGDYEASGYGYGPGVGEGYERIIMILSRASGAPVVAGAIRYA
                     ADLVRGDLKRVAAAEFAAARSAGWPALGEAPGSRAQQAVGGAEAGAGIAPPAAKVVTH
                     EIPGVDILQLEDAVQALWAAGIYAESGMGCTGPIVMVAQEDTDAAMGLLGKRGYL"
     gene            complement(3367..4944)
                     /gene="grdC"
                     /locus_tag="PLM74_08490"
     CDS             complement(3367..4944)
                     /gene="grdC"
                     /locus_tag="PLM74_08490"
                     /EC_number="1.21.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013239377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     component C subunit beta"
                     /protein_id="HON43150.1"
                     /translation="MDFAVVKGVSYALVHAPSLLVHMGTTQTVEREINPDSEYLNQFQ
                     KHLRSYQECVAYPANQVYIGNMTPAELANVARPWYERPVSGASRYGKWGEVMPEDEFY
                     GLMKIVDSFGLVVLEESFAREIAAKMSASPIWTDSDAARLGDGAPAAEVQATLDGGRA
                     EPLYVGDRLVGCVKAAHERDRALTAHVMVENLATKASGVLALKHLIKLNGLDAGEIDY
                     IVECSEEACGDMNQRGGGNFAKAIGEMAGCTGATGSDVRGFCAAPAHAMVYAASLVQS
                     GVFRNVVVVAGGATAKLGMNGRDHVKKGLPLLEDALGAFAILVSANDGVSPVIRTDAV
                     GRHTIGSGSSPQAVMTAIVLDPLERVGLKLTEVDKYSPEMQNPEITEPAGAGDVPKAN
                     FKMIAALAVKRGEIARTDIDSFVAAHGLPGFAPTQGHVPSGVPSIGYFRDEIMAGRMR
                     RAQIIGKGSLFLGRMTNLFDGVSFLFEKNKGMTEEKAQAQAQTQQGGAVATESRDELR
                     KIMAEALRELAQSLVKE"
BASE COUNT          965 a         1759 c         1291 g          949 t
ORIGIN      
        1 cacgcgtatc catattatgt tacccgccta tgcctgcgga tttgttgccg gcgtgactcc
       61 gaaaaacagg ggcgccgttg ccggcgcgaa ttcagataac tcgtacctgc caaaccaccc
      121 agaatccgag gaggttgatc aaaaagcctg ccggaaaacg aataggatga gccagaatct
      181 ggttgagcca aatgcgcctg tatcggggac caggtaatcc cactctacgg cgaataggat
      241 gccccggaac cctcgttaga gcccacacag gagaacgccg tacccagaaa tgttcacgga
      301 tgtgggtaat gcgtagccgt ttctggacag gttcttcgag catcctgttc agatcctgcc
      361 tggtttagaa ggcgacaatt gtccgaattc gcgccggcaa cggcgcctcc tttttctgtc
      421 atcgcgcgct cctcgaatcc gcaggcagag gcgggtgcaa taatatggat acacacctca
      481 atcccctaga tgcgcccaag aaggattcag acggccgcag attctccacg aaacgccaag
      541 aacttccgat ttctttctgc ccgttttcga aaccgggcac tttctgagca agtcgttcga
      601 aaaaccggac acattcacta caggttcgct aactcgctgc taaacgcggt ttcgcctaat
      661 ccccgcacaa tacggccacg aacaaccagc gtcttcgagc gaacgcgccc ggcaagactc
      721 gcatcctttc cggcgctgtg ggtaatgcac agagcagggg ccttctggcg agaatgatcc
      781 cctccaccac tctgtctacc aatgcctgtt tgcatttcca cttcccgatg cctacaggga
      841 ctacacgcgc ctcgaccggg ttgctacttc accgccgtaa tcatggggtc catctcgatc
      901 ggcgcgggca tctccacgac cacttgcacc agcgggctca gaagctcggt gaccccttct
      961 aggctggtag gccgatcctc gggcagcatg ctgccgaaca gcttccgcgc gaagtggcct
     1021 ccattgtggg tcgggcgaac aaccgagaac ccgatctccc taagccacct ggcatacgtt
     1081 gcgcccgacg gatggctcaa ggtgcacacc caggctcccg tgatcacagg ccgcagggca
     1141 tcaagcgcat caggtgttac gtgctcgatt cctacagcca tcccctcgga cgccgcggtt
     1201 gcgcccgccc gaccagtcag ccatgatgcg acttcgccga cggccatcga caaatccagc
     1261 ccgtactcat cggctcgttc ttgctcgacg tgcctgtcgt agatgatcag cctgcacgcc
     1321 tcacccatgt caagcaacca cacttctcgc tccttaccgc cgcggtagca gccaagcccc
     1381 tcatagtcca tccgaagacg cccccctggg cgcaacactc ggtagatctc ccgcaaggcc
     1441 tcacgggggt cgggcgtctg ctcaatcgaa gtggccgcca tgacaccatc gaacgactca
     1501 tcctcaaacg gcaatgggcc aacggctgga atgtggacga acctggcatt gtttatgccc
     1561 atgcgcgcgg cgttgccggc acatacctgc acacgccggg ccgaagcatc gacgcctacg
     1621 acttcgcccg cgaaaggtgc aacaggaagc gacggccacc catccccagg gccgaagtcc
     1681 agcagccggg cgccctgccc atgagtagcg aataagtagt ccagaatacg ccctcgatca
     1741 tcccagtgcc aggccttccc tggatcgaac gctttgtaga tcaccggaag gccatactcc
     1801 gactgcgact ccatcgcatc gtagaagaac ttatcagaag tacatcgctc gggcgcaaga
     1861 tgagtcgtga tccaccagat agcgtccatc gctcagcctc cccgttcagc cgtcatgtcg
     1921 agtcctattc ggttccgtat cgtgtgtccc acttcccggt tgataatacc cgaccctgtg
     1981 aactgccgta ggtaggccaa gtgaagaaaa tcccgccgcg ctacgcgcta atggaaagcg
     2041 cgagcgcggc gggttaccta caagctccac ttaccgggtt gctcaaggcc agctccaccc
     2101 atcaaccgtt gcttgggaca gtcgatacgg aagtctagag gtagcccctc ttgccgagca
     2161 gccccatcgc cgcatccgta tcttcctgcg cgaccatgac aatcggcccg gtacacccca
     2221 tgccgctctc ggcgtagatc cccgctgccc agagcgcctg caccgcgtcc tccagctgca
     2281 ggatgtcaac accgggaatc tcatgggtga ctaccttcgc cgccggcggg gcaatccctg
     2341 cacccgcctc agctccgccg acagcctgct gcgcccgcga ccccggagct tcgcccagag
     2401 ccggccaccc cgcgcttcgt gccgcggcaa actcggccgc cgccacgcgc ttcaggtcgc
     2461 cgcgcaccag gtcagcagcg taccgtatgg ctccggccac cacaggcgca cccgaagccc
     2521 gcgacaggat catgattatc ctctcgtaac cttcgcccac gccggggccg tatccatagc
     2581 cggatgcctc gtagtcgcct cctgtcgtga acgacgagaa caccttcatc aggagattcc
     2641 ccgtaagggt gtcgcacacc attacatcgg gaacccccac aagcaggtcg ttaccgcgca
     2701 tcactgcccc gccgtcggcc cgcgccgact cggcaaaccg cacatcatac ccgttgctgt
     2761 ccagtttcct cagggcccgc tccacctgcc gcgcgccgtc cacgttgaga acgccgacac
     2821 tgggctcggt cacgccgcac gccttggccg ccgcaatgcc gtacactgcg ttcttcacca
     2881 tgttctccac gcggtcagtt ccggacgttc ccgtagtcgt ggcgatgaac atctccctgc
     2941 ctcgccccgg tgtcaccact ctccccacag tcgatacccc tatagggaag gtgtagtgca
     3001 tcgtgacgca tgcatcaagt tggccgctat cgagcagctc ctccattcga gcatgctgcc
     3061 ctgcctcatc gctcgcccac accgcatcaa gcccccggcc ccgtgcatcc tcagggctca
     3121 ccgggcccac cagcacgacc tcgacatcgc tgtacgcacg cgccgcaagc tcagcgccgt
     3181 gcaccatttc gtccggcccg tgctcactgc cgagcacagt caggcccaca cgcggtctcc
     3241 gtccgaactg gccggtctcc agcccttcgg ccatctctcg aaagagctcg gccacagccg
     3301 ccatcccttt tgcccttgcc tctgcgcctg cccctgtcct tgcaccggct tcgctaccca
     3361 taggacctac tccttcacga gagactgagc taactcacgc agggcctcag ccatgatctt
     3421 gcgcagttcg tcgcgggatt cggtcgcaac cgccccgccc tgctgagtct gagcctgagc
     3481 ctgagctttc tcctcagtca tgcccttgtt cttctcgaac aggaacgaaa ccccgtcgaa
     3541 cagattggtc atccgcccta ggaagagcga gcccttgccg atgatctgcg cccgcctcat
     3601 gcggcccgcc atgatctcgt ctcggaagta gccgatgctg ggcactcccg agggcacgtg
     3661 gccctgtgtt ggggcaaagc ccggcaaccc gtgcgcggcc acgaacgagt caatgtcggt
     3721 cctggcaatc tctccgcgct tgacagcaag cgccgcaatc atcttgaagt tggccttggg
     3781 cacatcgccc gcgcccgccg gctcagtaat ttcggggttc tgcatctcag gcgagtactt
     3841 atcgacctcg gtcaacttaa gccccacgcg ctccagagga tcgagcacga tcgccgtcat
     3901 gactgcctgc ggcgatgacc ccgaccctat cgtgtggcgg cccacagcat cggtgcgtat
     3961 cactgggcta accccgtcgt tagccgacac caggatcgcg aaggcgccca gcgcgtcctc
     4021 gagtagtggc aggcccttct tcacgtggtc acgtccgttc atgcccagtt tagctgtggc
     4081 gccgcctgcg accacgacca cgttacgaaa cacacccgac tgcacgaggc tcgccgcgta
     4141 caccatggcg tgcgcaggcg cagcacagaa tccccgaaca tcgctgccgg tagccccggt
     4201 gcatccggcc atctcgccga tggccttcgc aaagttgccg ccgccccgct ggttcatgtc
     4261 tccgcatgct tcctcggagc attcaacgat gtagtctatc tcccccgcgt ccagtccatt
     4321 tagcttgatc agatgcttca gcgccagaac gcccgaggcc ttggttgcta agttctccac
     4381 catgacgtga gcggtcagag cccgatcacg ttcatgcgcc gccttgacgc acccgaccag
     4441 ccgatcccct acgtaaagcg gttcggcccg tcccccgtcc agtgtagcct ggacttctgc
     4501 ggccggggct ccatctccca agcgtgctgc atcagaatca gtccatatcg gcgaagccga
     4561 catctttgcg gcgatttctc tggcgaatga ctcctccagt actaccaggc caaaactatc
     4621 tacaatcttc atgagtccgt agaactcgtc ctccggcatg acctcgcccc acttgccgta
     4681 gcgcgaggcg ccactgaccg gtcgctcgta ccacggccgc gctacattcg caagctcagc
     4741 cggggtcatg ttgccgatgt agacctggtt agcaggataa gctacacact cctggtagga
     4801 gcgcagatgc ttttggaatt ggttcaagta ctctgaatcc gggttgatct cccgctccac
     4861 agtctgggtc gtgcccatgt ggaccaggag ggacggcgcg tgcaccagcg cgtagctcac
     4921 gccctttacg accgcgaagt ccatccaggc acctcctagt tcga
//
LOCUS       DAOTAC010000192         4949 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_14158_length_4949_cov_4.169391, whole genome shotgun sequence.
ACCESSION   DAOTAC010000192 DAOTAC010000000
VERSION     DAOTAC010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4949
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14158_length_4949_cov_4.169391"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="PLM74_08495"
     CDS             complement(<1..561)
                     /locus_tag="PLM74_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013514648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HON43151.1"
                     /translation="MNIRSVVSSVCRIVAVFGALMLAPAAVSLLFGESDWTAFLISSA
                     ITAAIGVVGALLAAPGTDWRIGNREGMAITTLGWTACAVFGALPFLLAGSLHASADAV
                     FETMSGLTTTGATVLLDVESQSHAILFWRSFLHWLGGMGIVVLSIALLPGLQVGGSQL
                     FRAEVPGPLPDRLEPRIRETAQRLWGI"
     gene            complement(573..1910)
                     /gene="trkA"
                     /locus_tag="PLM74_08500"
     CDS             complement(573..1910)
                     /gene="trkA"
                     /locus_tag="PLM74_08500"
                     /inference="COORDINATES: protein motif:HMM:NF007031.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HON43152.1"
                     /translation="MIGAGKLGFELASRLCQENRDVVVIDRAAERLGEVERNLDVMVI
                     HGNGASLRVLERAGVSSASLVIAVTDQDEVNMLACMLAKQAGAHTTIARVRDPDYDTD
                     LALASDDAYPGVDVTINPDHQAAADIARLIKNPDSSEFDYLAGGKVQILSYTVEPDSP
                     VAHTHVRELNIATATLVAILRNGDVLIPRGDTAILPGDKVFVVGRRGVPSVFGWRAGR
                     EATRLRTVSIIGGSSIGYYLAQAIEPLAAHGVQLKLFEKDHERCRTLAEAFPRLTVIQ
                     GDPSNLEVLQDENAESSDAAAVVGDNDQSNLLTGYLLKRMGVPRVVVSLKRTEYLPFT
                     GPMDLDATVVPRVSAAAAILKIIRGDHVLSLALLKEARAEVLEVLVSEECPACRIPLR
                     NLGVPAGAVIGVLVRGGQVIVPRGDTGMLPGDHVIVFMLESAREETARLFELR"
     gene            complement(2097..2639)
                     /gene="fabZ"
                     /locus_tag="PLM74_08505"
     CDS             complement(2097..2639)
                     /gene="fabZ"
                     /locus_tag="PLM74_08505"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: protein motif:HMM:NF000582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HON43153.1"
                     /translation="MFRRWEGPEAGPRAYCEPKARPEHAEGEHDAPANPISLLPHREP
                     FVLVDEIVSAEPGVQAVGIKRVRSDEFWATGHFPGHPVMPGVLIVEAMAQVGAVALFL
                     DMPQQDNSVLPLFAGANNVRFRHQVCPGDTLTIETRILQSWHSFGRAHSTARLPDGAL
                     AAEAELLFALEEAECKPSRG"
     gene            complement(2622..3869)
                     /gene="fabF"
                     /locus_tag="PLM74_08510"
                     /pseudo
     CDS             complement(2622..3869)
                     /gene="fabF"
                     /locus_tag="PLM74_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495369.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
     gene            complement(3917..4708)
                     /gene="fabG"
                     /locus_tag="PLM74_08515"
     CDS             complement(3917..4708)
                     /gene="fabG"
                     /locus_tag="PLM74_08515"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019542817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HON43154.1"
                     /translation="MAHDGRVALVTGGVRGIGLAIARRLAADGASVILNYRSSDPKVV
                     KSAVAAVSAASPESTVESTVTSASPGCRVCAIAADVSRHDEVRQMFAQVVSELGRLDV
                     LVANAGIVRDGLALSMEPESFMEVLQTNLVGAFLCAKEAARLMLKQRKGRIVFVSSIV
                     GLDGNAGQANYAASKAGLVGLAKSLARELGSRGITVNVVAPGFIETDMTASLPEEMRN
                     SCAARAPLRRIGSPAEVAAAVAFLASDEASYITGQVLRVDGGLTM"
     gene            complement(4708..>4949)
                     /locus_tag="PLM74_08520"
     CDS             complement(4708..>4949)
                     /locus_tag="PLM74_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007488088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43155.1"
                     /translation="AGIDTFIEIGPGKTLTGFVKKTLPHATTMNVEDLASLDALRAAV
                     SRARADRIAAASALAPAGVATAAVTAGAGAGGGVW"
BASE COUNT          914 a         1564 c         1531 g          940 t
ORIGIN      
        1 tatgccccag aggcgctgtg ctgtttcgcg gatccgcggc tcgaggcggt ccggcagcgg
       61 gccgggcact tcagcccgga acagctgaga acctccaact tgcaggcctg gcaggagtgc
      121 tatggagagc acgacgattc ccataccgcc gagccaatga aggaacgatc tccagaacag
      181 gatcgcgtgc gattgcgact ccacatccag cagtaccgtg gctccggtag tggtcaggcc
      241 cgacatggtc tcaaagacgg cgtcagccga cgcgtgcaaa gacccggcca acagaaacgg
      301 cagtgcaccg aagaccgcac atgccgtcca tcccagtgta gtaatggcca ttccctcgcg
      361 attgcctatg cgccagtcgg ttccgggcgc cgcgagcagc gcgccgacga cgccgatggc
      421 agcagttatc gccgacgaga tgaggaaggc cgtccagtcg gactcgccga agaggagcga
      481 cactgcagcc ggcgccagca tcaatgcccc aaatactgcg acgatgcggc aaaccgagct
      541 aacgactgac ctgatgttca atgcatacgc ctctatcgca gctcgaatag gcgagctgtc
      601 tcttctcttg ccgattctag catgaagacg atcacatgat cgccggggag catcccggtg
      661 tcgccgcggg gcacgatgac ctgcccgccg cggaccagaa ctccgataac agcgcctgcc
      721 ggcactccca gattgcgcag ggggatgcgg catgcagggc actcctccga gaccagcacc
      781 tccagcactt ccgcccgggc ctccttcagc agcgcaagcg agagcacatg gtcaccgcgg
      841 atgatcttga gtatggcagc tgcagcgcta accctcggta ccaccgtggc atcaagatcc
      901 atgggtccgg tgaagggcag gtactcggtg cgcttcaaag acacgaccac gcgaggcacg
      961 cccatacgct tcagcagata acctgtcagc agattcgact ggtcgttatc gcctactact
     1021 gcggctgcgt cagaactctc agcgttttcg tcttgaagga cctccagatt ggacggatcg
     1081 ccctgaatca cagtgagacg tgggaatgct tccgcaagtg ttctacacct ttcgtggtcc
     1141 ttttcgaaca acttgagctg tactccgtgt gcggccagag gctcgatggc ttgcgcaaga
     1201 tagtagccga tgctggaacc gccgattatg ctgactgttc gcagacgtgt agcctcgcgt
     1261 ccagcgcgcc atccgaatac gctgggcact cctcgccggc caaccacgaa taccttgtcg
     1321 ccgggaagaa tggctgtgtc gcccctgggg atgagcacat cgccgttacg caggatggca
     1381 actagggtgg cagtggcgat gttcagttcg cggacgtggg tatgtgctac cggcgagtcg
     1441 ggttcaactg tgtacgatag gatctgcacc ttgccgccgg ccagatagtc gaactcagat
     1501 gaatcggggt tcttgatcag gcgtgcaata tccgccgcgg cctgatggtc ggggttgatg
     1561 gtcacgtcaa cgcccggata cgcgtcgtcc gaagcgaggg cgaggtcggt atcgtagtca
     1621 gggtcgcgta cccgtgcgat ggtggtgtgc gctccggcct gtttggctag catgcacgcg
     1681 agcatgttga cctcgtcttg gtcggtgacg gctatgacca gtgacgcgct agataccccg
     1741 gctcgttcca gaacgcgtag agatgccccg tttccgtgga tgaccatgac gtccaggttg
     1801 cgctcaactt cgcccagcct ctcggctgct cgatcgatta cgacaacatc gcgattctcc
     1861 tggcacagcc ggctggcaag ttcgaatccc agtttgcctg cgccgatgat gatgactctc
     1921 gtatcgtatc actccagacc gcagatgcct ggcctgcgca tggcgcaatc cgctgcttaa
     1981 gtgaacccta tttgacgtcc ggtatcgctt atcctgcaag catgagaagg aaaggccctg
     2041 ctcgcgcagg gcctacctta gttcgcccgg aactcggtcg gccagcaccc ggtctgctag
     2101 cccctggacg gcttgcactc agcctcttcc agggcgaaga gaagctctgc ctctgctgcc
     2161 agggctccgt cgggcaggcg ggcggtggag tgagcgcggc cgaagctatg ccaggactgc
     2221 agtatcctgg tctcaatggt cagagtgtct cctggacaca cctggtggcg gaaccgcacg
     2281 ttgttcgctc ccgcgaacag cggaaggaca ctgttgtctt gttgcggcat atccaaaaag
     2341 agcgctaccg cacctacttg cgccattgct tcgactatga ggacgcccgg catcaccggg
     2401 tggccgggga agtgccctgt cgcccagaac tcgtcggaac gcactcgctt gatgcccacc
     2461 gcctgcactc cgggttctgc cgacacaatc tcgtctacga gcacaaaggg ctctctgtgg
     2521 gggaggagcg aaatggggtt agccggtgca tcgtgctcgc cctcggcgtg ctctggtcga
     2581 gccttaggct cgcaatatgc tctggggcct gcctccgggc cttcccaccg tctgaacacc
     2641 aggcagcagt tgtggccgcc aaaccccagg gagttgctga tggctgtgcg aacctcgagg
     2701 gggcgccctt ggttcggaat gtagtccagg ttgcattcgg ggtcaggctc ggcgtagtgg
     2761 atcgtcggag ggacgaattg gtcgacgatg gccccgatgc aggcgatggc ctccacggcg
     2821 ccggccgcgc cgagcaggtg gccgatcatc gacttggtgg agcttattgc cacgcgatgg
     2881 gcgtgttcgc ccagggcggt cttgatggcc tgcgtctccg tggaatcgtt gtagggcgta
     2941 cccgtgccgt gggcattcac atagtcgacg tccagcggcg aaattgaagc atcgcgcagc
     3001 gccgctatca tggcgcggga cgctccttcg ccgccggggg aagggctggt aatgtggtaa
     3061 gcgtcggtgg agagaccgca tccggcgatc tccgcgtaca cgcgggcgcc gcgggcgagc
     3121 gccgagtcga ggctctcaag caccagagcg ccggcgcctt cacccatgac gaagccgtcg
     3181 cgcccgaggt cgaagggccg aggtgcggca tccgggtcgt cgcttgtgga taatgcctgc
     3241 atgtttgcga atcctgcgat cgtcagcggg gtaatagccg cctctccgcc gccggctatc
     3301 atggcgtcgg cccgtccttc ggctatgagt cggaaggcgt ccgctatgcc gtgggctgat
     3361 gccgcgcacg ccgtaacctg gcacgtgctg gggcctttcg cgctgaacat tattgcgata
     3421 ttcccggcag ccatgttccc gatcatcatg ggcacgaaga acggactgac ccattcgggt
     3481 ccgcgctcga gatatcggct atgctgctct tcgaaggttt ccatcccgcc cacgcccgaa
     3541 ctgaaagcca cgcccagccg ctcaggcggg tagggatttc ggccggcgct gcctgccagc
     3601 ccagcgtttt ccatggcttg agcagcggcg accacagcgt attgagtgaa gcggtccatg
     3661 cgctttctgt ccttgcgatc gatataagga tcagggtcga atcccctgat ttctgcagcc
     3721 agtcttgccg ggcaggaagg cgcaggactg aaccgagtga ttctgccgat accggattgt
     3781 cccgcgcgcg cggcctccca gaatgtcttc catccacatc ccaggggcgt caccgcgccc
     3841 atcccggtga ccacaactcg tcttctcaag taagaacacc tctgttaggc ccgtgtgtcc
     3901 cagcgccgcg ggcggatcac atagtgagac ctccgtccac gcgcaggact tgacctgtga
     3961 tgtagctcgc ctcgtcggaa gcgaggaaag cgactgccgc agccacttcc gcgggtgatc
     4021 cgatccgccg caggggcgcg cgggcagcgc aggaattgcg catctcttcg ggcaaagatg
     4081 cggtcatatc ggtctcgatg aacccggggg ctaccacgtt cacagttatc cctctggacc
     4141 ccagctctcg cgcgagcgac ttggccaggc ctactaggcc ggccttgctg gctgcgtagt
     4201 tggcctggcc tgcattgccg tcaagcccga ctatggacga cacaaacacg atcctccctt
     4261 tgcgctgctt gagcatgaga cgggcggctt cctttgcgca caggaaggcg cccacaagat
     4321 tagtctgaag cacctccatg aaggactcag gctccatgga cagggccaga ccatcacgga
     4381 ctatcccggc attggccaca agcacgtcga ggcggcccaa ctctgagact acctgggcga
     4441 acatctgccg gacctcgtca tggcgcgata catcggccgc gatggcgcac acgcgacagc
     4501 cgggcgatgc cgacgtcaca gtcgattcca cagtcgattc aggtgacgcg gccgatacag
     4561 ctgcgactgc gcttttcacg actttcgggt ctgatgaccg gtagttcagg atcaccgacg
     4621 cgccgtccgc cgccagcctg cgcgcaatgg ccagtcctat cccccgcacg ccgccggtaa
     4681 ccagggcgac tctgccgtca tgcgccatta ccacacaccg cctccggcgc ctgctccggc
     4741 ggtgacggcc gcggtggcga cacctgccgg ggccagtgcc gacgcggcag caatccggtc
     4801 cgcgcgggca cgtgagactg cagctcgtag tgcgtccagt gacgcgaggt cctcgacgtt
     4861 catggttgtg gcatggggaa gcgtcttctt gacaaaaccg gtcagcgtct tgccgggccc
     4921 aatctcgatg aatgtgtcta tcccggcgc
//
LOCUS       DAOTAC010000193         4917 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_14276_length_4917_cov_4.637186, whole genome shotgun sequence.
ACCESSION   DAOTAC010000193 DAOTAC010000000
VERSION     DAOTAC010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4917
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14276_length_4917_cov_4.637186"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            325..1143
                     /locus_tag="PLM74_08525"
     CDS             325..1143
                     /locus_tag="PLM74_08525"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HON43156.1"
                     /translation="MPNMQVEDVSLFYEISGHGAETVAFLNGVAMSTERWAAQTPFFS
                     ENYRVLLHDFRGQGRSTLVSEGITFEMHARDMAALVDTLGIDRLHIVGVSYGAEVGMY
                     FALMYPERVKSLTLGTAVSESDPFLKAMIESWIEAARTYNGKLFFKVMAPTVYSKSFY
                     ATHKKWLDDYAELFGARVTREWMDAFAELCSNFLTLDMTDKLSEIRVPAFVISAAEDI
                     LKPPSYGRIIHEKIKDSRFAVVQGAGHALFLEKADEFNHLVSDFVQSNSMQSEE"
     gene            complement(1329..2351)
                     /locus_tag="PLM74_08530"
     CDS             complement(1329..2351)
                     /locus_tag="PLM74_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HON43157.1"
                     /translation="MDLMTVRKNAADRLTRSCHVCRQCNGVACAGQIPGFGGVGTGSG
                     FIANVEALAKKKLNLRVLHDARRPDMRISLFGIDLSMPILGAAVAGVRVNRMDGITEL
                     ELAKAMIGGPRLAGTIGMGGDGGDPEVFEATIAATAEAEGIAIPVIKPREQDALIERV
                     QRARDAGAVAVAVDVDAAALVNMALLGQRVEPKTVEQIREIVRAVDRPVILKGIMTPE
                     DAVIAAEAGAAGIVVSNHGGRALDHTPGTADVLPGIVRALRGGGRVAVLVDGGVRSGV
                     DVLKMLALGADAVLVGRPLAVAAVGGGAEGVRMQLEEYAKQLQVAMMLSGCASLADVT
                     GKVLFG"
     gene            complement(2434..3945)
                     /locus_tag="PLM74_08535"
     CDS             complement(2434..3945)
                     /locus_tag="PLM74_08535"
                     /inference="COORDINATES: protein motif:HMM:NF015563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HON43158.1"
                     /translation="MSEKTDSSVSIRIGKKAFISSLVILLALLVAAGLLTRVVPAGSY
                     VRQEDGGRTTIDISSFEYTDRPALPVWKWFTAPIEVLASPEGSVVIAIILFFLCIGGA
                     INLLNQCGVFSFIISRVAARYGARRYRLIASVVLVLMLMSALTGIMEEAVFIVPLMMP
                     LAAGLGMDPLVGLGMSFLALGFGFAAGLTNPFTIGVAQRVAQVPLFSGLWYRALVFAA
                     VYAVLSSFLIRHARRSEGPVRGHGQPMFDFGPTSCSSSSSGSSSNSGSGCSSGSSSNS
                     GSALVANKMPRASQWFMGAMAVMFVFSLSSSAVQGMSDLAFPVTTALFLIGGVGSALL
                     SGMGAGQVARTFGSGIVGMAPGIVLMLLAAGVKHVIASAGILDTIIFTGARIVEGMPR
                     VFAAGMIYLLVLIMNFFIPSASAKSLIMMPILAPLADVVGLNRQVAVLAFQFGDGFSN
                     VIYPTNPITLISLALAGVSYPRWLKQTIGLQLIVLAVTVLFLVAAVAIGLGPV"
     gene            complement(4028..>4917)
                     /locus_tag="PLM74_08540"
     CDS             complement(4028..>4917)
                     /locus_tag="PLM74_08540"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HON43159.1"
                     /translation="EAGDQGGIYEIAGRLGRASVRFEWVLDEGGVVSEGMMAGLDKPL
                     AVVGVAEKGYLDLELSVEMEAGHSSMPGRVTSVGLLSEAVARVEARQMPSRIVSPVRE
                     FFQQVGPYLGPAKRMVFANTSLFAPLIKHVLLGSPETAAMIRTTTAPTMISGGVKPNV
                     LAARASAVINVRLLPGDTARGVVRHVTNVVGDNRVKIKVLNESPASVVSPMGGPGYRL
                     IGECIRRYYPDAVVVPYLLTGSTDSKTLEPLASGVYRFTPCRLTKRDIARMHGVNERV
                     SIENIEKAVGFFKTIIAGA"
BASE COUNT         1065 a         1614 c         1313 g          925 t
ORIGIN      
        1 cacaggggaa cgtcacgccc agaaataccc atggatacgg gtaatgcaca gatggagatg
       61 ggttcatttc attatgggct tggttggcta tagcactggg gcgggcgctg agaggagtcc
      121 gacaacaggt accaagaagc tgcggttgcc gaggcatgga agctagcaac tgactgcggc
      181 atggctcaag cgcaactcaa catgggtcgg cgcaatgagg ccaaggccat ggccgaaagc
      241 gcggcggacg cctcgaccac atgtgtaacc cagatgtaac cgcatgggct caagatgaag
      301 caaacggatc ttggagggaa gggcatgccg aacatgcagg tcgaagatgt cagtctcttc
      361 tacgagattt cgggtcacgg tgccgagaca gtggcattcc tcaacggggt agcgatgtcg
      421 accgagagat gggcagcaca gactccgttc ttcagcgaga actaccgtgt cctgctgcac
      481 gatttccggg ggcaaggccg atctacgctg gtttcggagg gcattacgtt tgaaatgcac
      541 gctcgcgaca tggccgcact cgtcgacaca ctgggaatcg acagactgca catcgtgggc
      601 gtgtcgtacg gcgcagaggt cggcatgtat ttcgccctga tgtaccccga gcgtgtgaaa
      661 tcgctgacgc ttggcactgc agtgagcgag agtgatccat tcctcaaggc catgattgaa
      721 tcgtggatcg aagcagccag gacgtacaac ggaaagctct tcttcaaggt aatggcgccc
      781 acggtgtact ccaagtcgtt ctacgcgaca cataagaaat ggctggacga ctatgcagag
      841 ctattcggag caagagtcac tcgcgaatgg atggacgcct tcgccgaact gtgcagcaac
      901 ttcctcactt tggacatgac ggacaagcta tccgaaatcc gcgtacccgc cttcgtgatc
      961 agcgcagccg aggacattct caagccgccc agctacgggc ggatcatcca cgagaagatc
     1021 aaggactcca ggttcgcagt tgtgcaaggt gcaggtcacg ccctattcct agagaaggcc
     1081 gacgagttca accatctggt ctcggatttc gtccagagca atagcatgca gagcgaggaa
     1141 tagtcgccaa ataggccgca cgctgcgcat taggcgaaat ggtgccttta gctgggattt
     1201 cgtgatccgt ccgttttcag aacacattgt ccgacatgtg tcgcgaaatc ggacagtagc
     1261 tgatgcgtca gcctaaatcc catataaccc catgtccatc gcccctcaaa tcgccgccac
     1321 gctttcgtct acccgaaaag gactttgccg gtcacatccg caagcgatgc gcaccctgag
     1381 agcatcatcg ccacttgtag ctgctttgcg tactcctcca gctgcatccg cacaccttcc
     1441 gccccaccgc ccactgccgc aacagccaac gggcggccta ccagcacagc gtctgcgccc
     1501 agagccagca tcttgaggac atccaccccg ctgcgcacac cgccgtccac aagcacggct
     1561 acccgacccc cgcccctgag ggcacgtact atgccaggca gcacatccgc cgtgccgggt
     1621 gtgtggtcaa gggctctccc tccgtggttg gacacgacta tgcccgccgc gccggcctcc
     1681 gcagcgatca ccgcgtcttc cggcgtcatg atccccttga ggattaccgg cctatcaacc
     1741 gcgcgcacga tctcgcggat ctgctcgacc gtcttgggtt caactctctg cccaagcagg
     1801 gccatattca caagcgccgc cgcgtccacg tctacggcta cggcaactgc ccccgcgtcc
     1861 ctagcgcgct gaaccctctc tatcagggca tcctgctccc gcggcttgat caccggaatc
     1921 gcgatcccct cggcttcagc cgttgctgct atagtagcct cgaacacctc gggatcgcct
     1981 ccgtcgcccc ccatcccgat cgtgccggct agccgcggcc cgcctatcat tgccttcgcc
     2041 agttcgagct cggtaatgcc gtccatcctg ttcacccgca cgccggccac cgccgccccc
     2101 agaatgggca tgctcagatc gatcccgaaa aggcttatgc gcatatcagg gcgcctcgca
     2161 tcatgcagca ccctcaggtt gagcttcttc tttgcaagcg cctccacgtt agcgatgaag
     2221 ccgctgcccg tgcctacgcc tccgaacccc gggatctggc cagcgcatgc gaccccattg
     2281 cactgccggc aaacatgaca cgaccgcgtc aatctgtctg cggcattctt ccgcaccgtc
     2341 atcagatcca tcagcacacc tccgttccgt acccggcgcc tcccgcgctc tgccctattg
     2401 accagcgccc tagcgcgccc cgccccttca cgcctacacc ggtcccaagc ctatcgccac
     2461 cgccgccacc aggaacagga ccgtgaccgc cagcacaatg agctgaagtc cgatcgtctg
     2521 cttaagccac cgaggatagc tcactcctgc cagggccagc gagatcaggg ttatcgggtt
     2581 ggtggggtat atcacgttgc tgaaaccgtc tccgaactgg aatgcaagca cagctacctg
     2641 cctgttaagc cccacgacgt ctgccagagg agcgagtatc ggcatcataa tcagcgactt
     2701 ggccgaagct gatgggatga agaagttcat gatcagaacg agcaggtata tcatccccgc
     2761 cgcaaacact cggggcattc cctccactat ccgcgcgccg gtgaatatga tcgtgtcaag
     2821 tattcctgcc gatgcgatca cgtgcttcac tccggcagcc aggagcatca gcacgatacc
     2881 cggagccatg cccacgatgc cgctgccgaa agtcctggca acctggcctg cgcccatccc
     2941 cgaaagcaac gcagatccta cgccgcctat caggaacagc gctgttgtca caggaaacgc
     3001 cagatcgctc atgccctgca cggctgagct gctcaggctg aagacgaaca ttacggccat
     3061 ggcgcccata aaccactgcg atgcccgtgg catcttgttc gctacgaggg cggagccgga
     3121 gttggaacta gaaccggagc tacatccaga gccagagttg gagctcgaac cagagctaga
     3181 gctggaacaa gaagtagggc cgaagtcaaa catgggctga ccatgtccgc gtactggtcc
     3241 ctcgctccgc cgagcatgtc tgataaggaa ggaagacaga accgcataca ctgctgcaaa
     3301 gactaacgcc ctgtaccaca gtcctgaaaa gagcgggacc tgggctactc tctgcgccac
     3361 gcctatggtg aaagggtttg tgaggccggc agcgaacccg aacccaaggg caagaaaaga
     3421 catccctaac ccgaccaggg gatccattcc caggcccgct gcgagaggca tcatcagcgg
     3481 cactatgaag actgcttcct ccattatccc tgtcaaagcg ctcatcagca taagtacaag
     3541 tacaaccgac gcgattagcc ggtatctcct ggctccgtat cgcgccgcga ctcggctgat
     3601 tatgaaggaa aacaccccgc attggttcaa caggttaatg gcaccgccga tacacaggaa
     3661 aaacaggata atcgcaatca ccaccgagcc ttctgggctt gctagcacct cgatgggcgc
     3721 ggtgaaccac ttccacacag gcagggcagg acggtcagta tattcaaacg agctgatgtc
     3781 gatagtcgtc cgaccgccgt cttcctgacg tacatagctc ccagcaggca caacccgtgt
     3841 gagcaaaccg gccgccacca gcaaagccag gagaataacg agcgatgaga tgaacgcctt
     3901 tttgccgatt ctgatagaga ctgatgaatc tgtcttctcg ctcaagcggg ttccgccccc
     3961 ttcgtctgtc tgtccagcgc agtccggact actcgccctt gccgcgctcc cttgccgttg
     4021 cggcgcgtta tgccccggct atgatggtct tgaagaaccc gaccgctttc tcaatattct
     4081 cgattgacac gcgctcgttc acgccgtgca tgcgcgcgat gtctctcttg gtcagcctgc
     4141 acggcgtgaa tctgtacacc cccgatgcca ggggttccag cgtctttgag tcggtgctgc
     4201 cggtgagaag gtatggcacg accacagcgt ccgggtagta tcgcctaatg cactctccga
     4261 taagccgata cccagggcca cccatgggcg atacgaccga tgcggggctc tcgttgagca
     4321 ccttgatctt gacccggttg tcgcccacta cattcgtaac atgcctgacg acgccccggg
     4381 ccgtgtcacc cggaagcagc cggacgttga tgaccgccga tgctcgcgca gccaatacgt
     4441 tgggcttcac gcctcccgag atcatggtag gcgccgtcgt agtcctgatc atggctgctg
     4501 tttccggaga gcccagcagc acatgcttga tcagcggcgc aaacaaggac gtgttggcga
     4561 agaccatccg cttcgcgggc cccaggtatg ggccaacttg ctgaaagaac tcccgcacag
     4621 gactgacgat tcgtgaaggc atctgccttg cctccacccg cgcaaccgcc tctgatagaa
     4681 ggcccacgga cgtcactcgc cccggcatgg acgaatgccc ggcttccatc tccactgaaa
     4741 gctcgaggtc aagatagccc ttctctgcca cacccaccac tgccagcggc ttgtccaggc
     4801 ccgccatcat cccttcgctt actaccccgc cctcatccag cacccactcg aagcggacac
     4861 tggcgcggcc caagcgcccc gcgatctcgt agatgccgcc ctgatctccc gcctctt
//
LOCUS       DAOTAC010000194         4914 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_14290_length_4914_cov_4.816217, whole genome shotgun sequence.
ACCESSION   DAOTAC010000194 DAOTAC010000000
VERSION     DAOTAC010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4914
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14290_length_4914_cov_4.816217"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            657..1757
                     /locus_tag="PLM74_08545"
     CDS             657..1757
                     /locus_tag="PLM74_08545"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020618239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HON43160.1"
                     /translation="MSNHTGTRTEAAEAIEAIASVFRTHHEQRHFSGAGLVKRGDEVL
                     FSGAYGYAHRGWRVPNTVCTRFDTASITKLFTAVAVLQLVERGLLSLHDRVLDILKLP
                     GTTISPRVDVYHLLTHSSGIGDDADEEAGESYEAIWTDKPNYLVRETVDFLPQFVHKP
                     ANFEPGDGCRYNNCAFVLAGLVVEKLTGQKYRDYVRENVFRRAGMSDTEFVAMDTVAE
                     NVAEGYSAVTDDDGKLVGWRKNIYSFPPIGSPDAGAYTTVGDLDVFMRALTGSKLLSK
                     QLTEDMLTPKVMYRQTARATYMMGYAFEFALEPGSGRIMYMQKDGDNPGVDGVLRYYP
                     DTSTVVAILANQDCNVWDMANEVDGALEGLLV"
     gene            complement(1796..2680)
                     /locus_tag="PLM74_08550"
     CDS             complement(1796..2680)
                     /locus_tag="PLM74_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007930961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HON43161.1"
                     /translation="MEFIPAKTIISGYAEGSSWFGNNYNMNIYKGCCHGCIYCDSRSE
                     CYRIEDFDQVWAKDNALALIARELRSKRRKGVVGTGSMSDPYNPFEQEHRLTRGALEL
                     VDAHGFGASIATKSDLVTRDIDVLTSIKTHSPVLVKITVTTADDELCRKIEPRAPLAS
                     RRFSAIRQLAENGIFAGILLMPVLPFIEDNDDNVVSIIRLASEAGAGFVYPAFGVTLR
                     QNQREWYYSKLDEHFPGLRLKYAKQFGSSYECRSPRASELWRVFCAECDRLGILYKME
                     DIIEAYRQPYSRTQLSLF"
     gene            complement(2744..3271)
                     /locus_tag="PLM74_08555"
     CDS             complement(2744..3271)
                     /locus_tag="PLM74_08555"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HON43162.1"
                     /translation="MALELLTVSSRKMTPSQIGDVIQLCSRVFQCDYSYYMNLPLDRV
                     HVLGYIDGLLVSHALWLDRRLRIGSGPWLNTAYVEGVATREDCRKQGFGSALMRRLQR
                     EISGYDLGALSPSRPEWYERLGWIRWQGPLRIVKDGGILETPEECVLVYGTPITGQID
                     VTSPLTGEWRPFELW"
     gene            complement(3513..4163)
                     /locus_tag="PLM74_08560"
     CDS             complement(3513..4163)
                     /locus_tag="PLM74_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43163.1"
                     /translation="MFVDILPPRGLEVLDLLSATLPMERFYLAEGTGLALQLGHRKSY
                     DLDFFSQAAFDTFDIIQALSVAGHFRLTGEAIGTVHGFLNDVRVTFLHYPYGLLFPPT
                     AFNRISIADPRDIGLMKITAISGRGSKKDFIDLRFISRQVIPLESLFGLMEKKYLGVD
                     YSEYHLIRSLAYFDDADREPDPIMRSGVSWSETKDYFRAQQRSLIRKYLRPDDIGS"
     gene            complement(4121..4393)
                     /locus_tag="PLM74_08565"
     CDS             complement(4121..4393)
                     /locus_tag="PLM74_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43164.1"
                     /translation="MAKFFWDTDPMHLDVDADYFFIIERLMEEGDDSAIQWMMHRYGD
                     AQIVEVVRGSRQLSRKTARLWQNYYDLGEEEVKCLSTSYPRVGWRF"
     gene            4723..>4914
                     /locus_tag="PLM74_08570"
     CDS             4723..>4914
                     /locus_tag="PLM74_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43165.1"
                     /translation="MINPWKRVLVVALALCAFVMLVSHGQLIPVGCASTYDYGSRLKQ
                     VREFDEVRGCDLSGIEGVIT"
BASE COUNT         1044 a         1350 c         1449 g         1071 t
ORIGIN      
        1 aaccacccag aatccgagga ggttgatcaa aaagcctgcc ggaaaacgaa taggactagc
       61 cagaatctgg ttgagccaaa tgcgcctgta tcggggacca gggttacccc actctggggc
      121 atcctgtcca tttttgaacg ggcttttcga ggatcctgtt cagatcctgc ctggtttggc
      181 aggcgacaat tatcagaatt cgggcctgca attgccaatc ctttttctgt catcgcgtcg
      241 tcattgaatc cgcaggcaga ggagtctggc ataatctggc catgcgcctc tttccatcga
      301 gtatgcccga gaaggaatta ggcggccgca aagtctctat gaaacgccag aaactcccgt
      361 gttttgcctg ctcccggtcg aaaccgggca tttcctgagc aggtcagcgt acaaaccgta
      421 cagtgttgtt acagatgagc ggaccccctg ctaaatgcgg tttcgcctag aggcctgtgc
      481 aacgtggctt tcaccgacaa ctgcgcgtgt ccaagcgtag gcaagccccc ggcgcgatgc
      541 gttcggccaa taagcaagac tcgcaacctt tccagcgatg tgggtaatgc atagcggcca
      601 cacgacaggc tggtacgggc agagttcgga tggctgacag aatggatgtg acatgaatgt
      661 ccaatcacac aggaacaaga accgaggctg cggaagccat tgaggccata gcctcagtct
      721 tccggaccca ccatgagcag cgccatttct ccggcgcagg gctcgtgaag cgtggcgatg
      781 aggttctgtt ttctggcgcg tatggatacg cgcacagagg ttggcgagtc ccgaataccg
      841 tgtgtacccg gttcgacacg gcctccatca ccaagttgtt cactgcagtg gctgtgctgc
      901 agctagtgga gcgtgggctt ttgtcactgc atgatagggt gctcgacatc ctcaaacttc
      961 cgggaactac gatttcgccg cgggtcgacg tgtaccacct gctcactcat tcgtctggaa
     1021 taggcgacga cgctgacgag gaggcggggg agagctacga ggccatttgg acggacaagc
     1081 caaactactt ggtaagggag actgtggact tcctgccgca gttcgtgcac aagccggcca
     1141 acttcgagcc gggcgatgga tgcaggtaca acaactgcgc gtttgtgttg gccggcctgg
     1201 tggtggagaa gcttacgggc cagaagtatc gagactatgt ccgtgagaac gtgttcaggc
     1261 gggcgggtat gtccgacact gaattcgtgg caatggacac ggtagccgag aacgtagcgg
     1321 agggctactc cgctgtgaca gacgatgacg ggaagctggt cggctggcgg aagaacatct
     1381 actcgtttcc tcccataggc tcgcccgacg cgggcgcata cacgacggta ggcgatctgg
     1441 acgtgttcat gagggcgctc acagggagca agttgctatc gaagcagctc accgaagata
     1501 tgctgactcc caaggtaatg tacaggcaga ccgcgcgcgc cacgtacatg atgggatatg
     1561 cattcgaatt cgcgctcgag cctggcagcg gcagaatcat gtacatgcag aaagacgggg
     1621 ataaccccgg ggttgacggc gtgctaaggt actaccctga taccagcact gtagttgcga
     1681 tcctcgcgaa ccaggactgc aatgtatggg atatggcgaa cgaggtggat ggggcgctcg
     1741 agggcctgct cgtctgattg gcacgtccat cgtctggttt gtggcgtggt cctgttcaga
     1801 acagcgagag ctgagtcctg ctgtatggct gacggtaggc ctcgatgatg tcttccatct
     1861 tgtacaggat gcccagccta tcgcactccg cgcagaacac gcgccacagt tcacttgccc
     1921 tgggagagcg gcattcgtac gaactgccga attgcttagc atacttgagc ctgagaccgg
     1981 ggaagtgttc atcgagtttc gagtagtacc actctcgctg gttctgtcgc aatgtgactc
     2041 cgaaggccgg atagacgaac cctgcgccgg cctcgcttgc tagccggatg atgctgacca
     2101 cgttgtcatc gttatcctct atgaacggca gtaccggcat gagcagtatt cctgcgaaaa
     2161 tgccgttttc ggcaagctgc ctgatggcag agaatcgcct ggaggccaag ggcgcgcgcg
     2221 gttcgatctt gcggcacagt tcatcatcgg ctgtggtgac ggttatcttg actagcacgg
     2281 gcgaatgagt cttgatcgag gtgagcacgt cgatatcccg cgtcaccaga tcgctctttg
     2341 ttgctatcga agccccgaag ccgtgcgcgt ctacgagctc cagcgcgcct cgcgttaggc
     2401 ggtgctcctg ctcgaaaggg ttgtagggat cactcattga gccggtgccc acaacgcctt
     2461 ttctgcgttt gcttctcagc tcccgtgcga tcagggcgag ggcgttatcc tttgcccaca
     2521 cctgatcgaa gtcctcaatc cggtagcact cactgcggct gtcgcagtag atgcacccat
     2581 ggcagcagcc cttgtagatg ttcatattgt agttgttgcc aaaccacgag ctgccctcgg
     2641 cgtagccgga gatgattgtc tttgcgggga tgaactccat gctcaggatc ccttccatat
     2701 ggtcagtcgc ggggaccgga ggcgttgccg acgcactggt aggctaccac agctcgaacg
     2761 gtcgccactc tccggtgagg ggcgatgtga cgtcgatctg ccccgtgatg ggagtgccgt
     2821 acaccagcac acactcctcg ggcgtttcga gtatgccgcc gtctttcact atccgaagag
     2881 gcccctgcca gcggatccag ccgaggcgct cataccactc cgggcggctg ggtgaaagcg
     2941 cacccagatc atagccagat atctcgcgct gcaggcggcg catcagggct gaaccgaatc
     3001 cctgcttgcg gcagtcttcg cgagtcgcga cgccctcgac atatgccgtg ttcagccacg
     3061 gaccgctgcc gatacgcagg cggcggtcga gccatagggc gtgcgatacc agcaggccgt
     3121 cgatgtaccc tagcacatga actcggtcaa gcggcaggtt catgtagtat gagtaatcgc
     3181 actggaacac ccgcgaacac agctggatga cgtcaccgat ctgcgacggc gtcatcttcc
     3241 gcgaagagac ggtgagcaac tccagagcca agctgcatag acctcctttg tgggcgcctc
     3301 ggcggacacc tgccgggcac gggccttcta gaacgtcggt cttcgggaga tctttcgcca
     3361 gtgacagatg gggttactta ccgcctattt ggcggacacg cccaagcgaa ggaaggcgta
     3421 tgacaaagta gccttgggca gatttgcggg cggggtgatg ttcttcttcg ccggatgcct
     3481 gctactgctc atggccggaa tcgtagaagc gatcaactac cgatatcgtc aggcctaagg
     3541 tacttccgaa tgagcgacct ctgttgagcg cggaagtagt ccttggtctc gctccaggaa
     3601 acgcctgatc tcatgatggg gtcgggttct ctgtcggcat catcgaagta cgccaagctg
     3661 cgaattaagt ggtactcact gtagtccaca cctagatact tcttctccat tagcccgaat
     3721 aggctttcga gcggaatcac ctgccggctt atgaaacgca agtcgatgaa gtccttcttg
     3781 ctgccccggc ccgaaattgc ggtgatcttc attaggccga tgtctcgtgg atcagctatc
     3841 gagatgcggt tgaatgcagt gggcgggaag agcagtccat acggatagtg aagaaacgtc
     3901 accctgacat cgttcaggaa gccgtgaact gtgccaatgg cttcaccggt gagacggaag
     3961 tggccggcta cactcaaggc ctggatgatg tcgaatgtgt cgaacgcggc ctgtgagaag
     4021 aaatctaggt cgtaggattt cctgtgaccc agttggagag ccagtcctgt gccttcggct
     4081 aggtagaacc gctccatcgg cagtgttgcc gaaagaaggt ctagaacctc cagcccacgc
     4141 gggggtagga tgtcgacaaa cacttgacct cctcctcacc aagatcgtag tagttttgcc
     4201 acagtcgcgc cgtctttcgc gacagctgtc tactgcctcg cacaacctcg actatctgag
     4261 catcgccgta gcgatgcatc atccactgga tcgcagaatc atcgccttcc tccatcagcc
     4321 tctctatgat gaagaagtag tccgcatcga catccagatg catagggtcc gtatcccaaa
     4381 agaacttcgc cactgagcgt ggaggagtct ccatgatgca tcgccccatt ctgcgatcag
     4441 tctgtcactg cctaatgatg tcgcatttcg atggtaccat cttcccgcgt gcggcgcaac
     4501 aagcctattc acagcgcgtc catctgatgg ggttgtacag gcggtcgtaa cagcgattga
     4561 ctccgctggc gtcgggatgt cgaccttgaa gatgagtttc cgagcaatcc tctcgggccc
     4621 tgacggcttt ctcggcagga caaatctgac aggcagagta tacccgaaga gacaaggatt
     4681 gccgattcac ctgagctacc tgagtcgaag ggagcgagag aattgattaa tccatggaag
     4741 cgcgttcttg tggttgcgct agcgctctgt gcctttgtga tgctggtctc gcatggtcag
     4801 ctgatccctg ttggatgtgc ttccacttat gactacggca gcagactaaa gcaggtaagg
     4861 gagttcgacg aggtaagggg gtgcgatctt tcgggcattg aaggtgtcat cacg
//
LOCUS       DAOTAC010000195         4878 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_14414_length_4878_cov_4.578685, whole genome shotgun sequence.
ACCESSION   DAOTAC010000195 DAOTAC010000000
VERSION     DAOTAC010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4878
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14414_length_4878_cov_4.578685"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /gene="fliF"
                     /locus_tag="PLM74_08575"
     CDS             complement(<1..797)
                     /gene="fliF"
                     /locus_tag="PLM74_08575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HON43166.1"
                     /translation="MTLPLPQVGEQAGAAGQAGTGRAGRGGDRAEGGGSSGEAGGSGR
                     PGGAGDRSGSGGRGGRGGRGDHGGLTTTQKIIIGVVAGGVFIALIMLIVSSSTSDFAV
                     LYSGLPPEDAGRIVEYLKESGVKYKLSSGGSTVMAPSNRVYELRMELASKGVPQGAGV
                     GFELFDKTNFGATDFTQKVNYARALQGELARTIMALNPVELARVHIVIPEERLFSEQQ
                     KPTTASIVLKLKPGAYILDAQVRGVVNLVAGAVQGLSPEFITVVDTDG"
     gene            complement(802..1125)
                     /gene="fliE"
                     /locus_tag="PLM74_08580"
     CDS             complement(802..1125)
                     /gene="fliE"
                     /locus_tag="PLM74_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HON43167.1"
                     /translation="MAGIEGMGLRGRLTAGAATASAATGVSSDGQTDLVSAFGGVLEQ
                     ALDTVNDMQTRADRLTEKLAAGEVRDIHQVMIAVEQVNIALQLTMQVRNKVIESYQEI
                     MRMQV"
     gene            complement(1163..1558)
                     /gene="flgC"
                     /locus_tag="PLM74_08585"
     CDS             complement(1163..1558)
                     /gene="flgC"
                     /locus_tag="PLM74_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HON43168.1"
                     /translation="MSVISTFGTSASGMAAERLRLDTISSNLANINTTRTPEGGPYRR
                     LMAVMESAPDGRGVHVARIVQDESPPLLVHNPGHPDADAEGNVAMPNVNPVLEMVDMI
                     SATRAYEANVAAFNAAKAMAAKALEIGKA"
     gene            complement(1562..1993)
                     /gene="flgB"
                     /locus_tag="PLM74_08590"
     CDS             complement(1562..1993)
                     /gene="flgB"
                     /locus_tag="PLM74_08590"
                     /inference="COORDINATES: protein motif:HMM:TIGR01396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HON43169.1"
                     /translation="MKILDDRTMRTLETALDVASARHQVIANNIANVNTPGFKAADVD
                     FVSSLEQAMAGRAEPTGPKLTGRATRVGHLPISGPASAGQLPVVQTVDEGASMRVDGN
                     SVDIDLEMAKMAENATMYNTFAQLVSSKFSLLKYVISEGRR"
     gene            complement(2175..3995)
                     /locus_tag="PLM74_08595"
     CDS             complement(2175..3995)
                     /locus_tag="PLM74_08595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HON43170.1"
                     /translation="MRDMEVRLWAPSAVIMTIAGIRLVCSWVSPIAHGWWTVAGDVLL
                     LSSVALLSAHYIAVIRRRTREAGIAADRVSRLEMVTRELVDHEAMIRDDLVRGQRELN
                     ERIVRLSGILDASRDLVATRDFREVLERITEMARSVVGADWASLTVDACLAGTSATGG
                     DTVVITAGAPEGTSSLFHLSTPIVCLGQISGQLTAHFSAVRPGASEVTHLLSIISSFA
                     GVAMENARLYHDLSGANLRLEAARRYAERLIEEVPVGIVVVDRDYHVMTWNRVLSNTF
                     RVPEEKVFGRHLADIVGTDEHRLVQMLSEAAEDARSMSPRNIVLTDAEGGRHTMRLVV
                     SAVDSLVGGDEGLIIMAEDVTERVRLGEQLKQAERLRTLGEFAAGMVHEINNPIGIIS
                     ACAEVLGRKLARHGDEFHEYVRSAKIIEDEAIRCSSIVRNLLSFAREAEMNPVEVNVA
                     ALLRRTSEFVKGKASELGVELILDIPDNIPVIIGDRDQLEQVFLNLAVNGIEAMKGGG
                     RLHVSVARRDNEMRVSFTDTGPGIPAQDIPHVFNPFYTTKPSGTGLGLALSWSIIERH
                     GGTIEVDNVSGGGGARFEVILPLQWSLGREQTTRQMLPGR"
     gene            complement(4022..>4878)
                     /locus_tag="PLM74_08600"
     CDS             complement(4022..>4878)
                     /locus_tag="PLM74_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HON43171.1"
                     /translation="AKLLRVLQEKEFERVGGVDTIKVDVRVVAATNQKLKQAVAEGAF
                     REDLYYRLSVVPLFLPPLRERKQDIPALVSRFIHKYSQEFGRSVSGITHEALEHLAAH
                     NWPGNIRELENALERAVLLARRDGLTSAVFHLGAEPMHVIPFGLGSQLLADSVLRDQG
                     EPGYAGEEGDAREAGNNREAGEARDAGDTGSAGNAGNAGNTGSAGDASDMGDVDDTGD
                     DGEIDVPGDAYGFDNDDQQGAMSLEELERRYIEQVLEQCGDNRTHAARILKITRRTLL
                     NKIKKYDL"
BASE COUNT          970 a         1623 c         1263 g         1022 t
ORIGIN      
        1 ccgtcggtat ccacaacagt gatgaactcc ggcgatagac cctgcacagc accggccacc
       61 aggttaacca caccgcgcac ctgcgcgtcg agtatgtagg caccgggctt gagcttgagg
      121 acaatcgatg cggtagtggg tttctgctgt tccgagaaga gcctctcctc ggggatgacg
      181 atgtgcacac gcgctagctc aacagggttg agcgccatga tcgtgcgcgc cagctcgccc
      241 tggagagctc gagcgtagtt gaccttctga gtgaagtcag ttgcgccaaa gttggtcttg
      301 tcgaacagct cgaaccctac ccctgcgccc tgcggaacgc ctttgctcgc aagttccatg
      361 cgcaattcgt acacgcgatt cgacggcgcc atgaccgtgc tgccgcccga cgacagtttg
      421 tacttgacac cgctttcctt caggtactcg acgatcctgc ccgcgtcctc cggcggcagg
      481 ccggaataaa ggacggcgaa atcgctggtg gaactggaga cgataagcat gatgagagca
      541 atgaagactc caccagctac cacgccgatg atgatcttct gcgtggtggt cagtcctccg
      601 tgatcgcccc ggccgcccct gccgccgcgt ccgcccgacc cactgcgatc gccggctcct
      661 ccaggcctgc cggatccgcc ggcctcgccg gatgatccgc cgccttccgc gcggtcgccc
      721 ccccgtccgg ctcgcccggt gccggcctgc ccggcggcgc ctgcttgttc gcctacttga
      781 ggtaatggca gagtcattca ctcatacctg cattctcatg atttcctggt atgactcgat
      841 gaccttgttc ctcacctgca tggtgagctg aagagcgata ttcacctgtt ccacagcgat
      901 cataacctga tgtatgtcgc gaacctcgcc ggcggcgagc ttctcagtca gcctgtcggc
      961 ccgtgtttgc atgtcgttca cagtatccag ggcctgttcg agcacgcctc cgaaggcgct
     1021 gaccagatcg gtctgaccgt ccgacgacac acccgtcgca gctgatgcag tcgctgcgcc
     1081 cgcggtcagc ctgcctctca gccccattcc ttcaatccca gccacgaggt cgcacctcca
     1141 atgtcatgtg tgaatgaaat gactacgctt tgcctatctc cagcgccttg gccgccatag
     1201 ctttggccgc attgaacgcc gccacgttag cctcataagc cctagttgcg ctgatcatat
     1261 ccaccatctc cagcaccggg ttgacgttgg gcatggccac attgccctcg gcatcagcgt
     1321 ccgggtgacc aggattgtga accaagagcg gcggcgactc atcctgaact atgcgcgcga
     1381 catgtacgcc gcgcccgtca ggcgccgatt ccatcacagc catcaggcgc ctatagggtc
     1441 caccctcagg agtgcgcgtt gtgttgatgt tggccaggtt gctcgatatc gtgtcaagac
     1501 gcaatctttc cgccgccatc cccgatgcgg aagtgccgaa tgtggatatc acggacacag
     1561 actaccgcct cccctcgctg atcacgtatt tcaaaaggct gaacttgctc gacactaatt
     1621 gggcgaaggt gttgtacatc gtcgcgttct ccgccatctt cgccatttcg agatcgatat
     1681 cgacgctgtt tccatctact ctcatcgaag caccctcgtc cactgtctgc acaaccggca
     1741 actgccccgc tgacgcaggg cccgaaatcg ggaggtgccc gacccttgtc gctcggcctg
     1801 tgagcttggg accagttggc tccgcgcggc ctgccatcgc ctgctcaaga ctgctcacga
     1861 agtctacatc cgccgcctta aaccccgggg tattaacgtt agctatgttg ttagctatca
     1921 cttggtgacg tgcggacgca acatccagcg ccgtttcaag ggttctcatc gtgcgatcgt
     1981 cgaggatctt caactcacac attctccctt cggaaagaca atagaaaccc cgttaaattg
     2041 tgatgcaata tcgatgccac tcaagaaaca ggcacaaaaa agaacagccg ccctcgaact
     2101 tccgagaagt ggctgctaca agccgtattg cgcaggctct gcgtcgcaag cctccgccgg
     2161 tgagtagttt ttgctcaccg tccgggcagc atctgccggg tagtctgttc tcgccccaag
     2221 ctccactgca agggcaggat cacttcaaac ctggcgcctc cgccgcccga cacattgtct
     2281 acctcgatgg tgcctccatg cctttcgata atgctccaac tgagagcaag gcctaggcct
     2341 gtgcctgacg gcttagtggt gtagaacggg ttgaacacat gaggtatgtc ctgagccgga
     2401 attcccgggc ctgtgtcggt aaatgagacc ctcatctcgt tatccctccg tgcgacggac
     2461 acatgcagcc tgccgcctcc cttcatggcc tcgatcccgt tcacggccag gttcaggaag
     2521 acctgctcca actgatcccg gtcgccgatg ataaccggga tgttgtctgg tatatccagg
     2581 atcagctcga ctccgagctc ggacgcctta cccttcacga actcggaggt acgccgcaga
     2641 agcgccgcga cgttcacttc gaccgggttc atctccgctt ccctcgcgaa actgagaagg
     2701 ttcctcacaa tagacgaaca acggatcgcc tcatcctcta tgatcttggc ggaccggaca
     2761 tactcgtgga attcatcacc atgccgcgca agtttccgcc ccaggacctc agcgcaggct
     2821 gagataatcc caatggggtt gtttatttcg tgcaccatgc ccgcagcgaa ctcgccgagc
     2881 gtacggaggc gctcagcctg cttgagctgc tcgccaaggc gcacgcgctc agtaacatct
     2941 tcggccatga ttatgaggcc ctcatcccct ccgaccaacg agtccactgc cgaaacaaca
     3001 aggcgcatcg tatggcggcc accctccgca tctgtcagaa caatattgcg tggggacatg
     3061 gagcgcgcgt cctcggcagc ctcagacagc atctgaacta gcctgtgttc atcggtccct
     3121 acaatatctg ccaagtgccg cccaaatacc ttctcttcag gcaccctaaa ggtgttcgaa
     3181 agcacgcgat tccaggtcat gacatggtag tcgcggtcga cgacgactat ccctaccgga
     3241 acttcctcga tgagcctttc agcatatcgc ctggccgcct caagcctcaa attggcgccg
     3301 ctcagatcat ggtaaagacg ggcgttttcc atggccacgc ccgcaaacga agagatgatg
     3361 gacagaagat gcgtaacttc cgaggcacca ggacggacgg cgctgaaatg cgccgtgagc
     3421 tggccactta tctgaccgag gcatacgatg ggcgtggaca agtgaaatag cgaagacgtt
     3481 ccctccggcg cgcccgccgt gatgacgacc gtgtcgcctc cagtcgcaga tgtgcccgcc
     3541 aaacatgcgt ccaccgtgag agatgcccag tcggcgccga caacgctcct tgccatttcg
     3601 gttatgcgct ccagcacttc gcggaagtcc ctagtcgcca ccagatcacg ggaggcgtcg
     3661 agtatgccgg acagcctgac gatgcgctcg ttcaactccc gctgcccccg cactaggtcg
     3721 tcgcggatca tggcctcatg atccacaagc tccctggtga ccatctcaag ccgactcacc
     3781 ctgtcagcag cgattccggc ctcccgcgtt cggcgacgta tgactgcaat atagtgagcc
     3841 gatagcagag caactgacga aagcagcaac acgtcgcccg ccaccgtcca ccagccgtgg
     3901 gctattggcg acacccatga gcacaccagt cgaatcccgg cgatcgtcat gatcaccgcg
     3961 gaaggcgccc acagccgcac ctccatgtct ctcataaggc atcacatccg catacgcttt
     4021 atcagaggtc gtacttcttt atcttgttga gcaacgttcg acgggtgatt tttaggattc
     4081 ttgccgcatg tgtccggtta tccccacatt gctcaaggac ctgctcaata tagcgacgtt
     4141 ccagttcctc cagcgacatc gcgccctgct gatcatcatt gtcgaaccca tatgcgtccc
     4201 ccggaacgtc aatttcgccg tcatcgcccg tatcgtccac atcacccatg tcgctcgcat
     4261 cgcccgcact gcctgtgttg cccgcattgc ctgcattgcc tgcactgccc gtgtcccccg
     4321 catcgcgtgc ttccccggct tcccggttat ttcccgcttc ccgggcatcc ccctcttccc
     4381 ccgcataccc gggctccccc tggtcgcgca acacggaatc ggctaacagc tgcgagccca
     4441 acccgaacgg gattacgtgc atgggttcag ccccgagatg aaaaacagca gaagtcagcc
     4501 catccctgcg cgccaaaagc acggctcgtt ccagagcatt ctctagctcc ctgatgttcc
     4561 caggccaatt gtgagctgcg agatgctcca gggcttcgtg ggtaatgccc gatacactcc
     4621 taccgaactc ctgactgtac ttgtgaatga accgggagac cagagcggga atgtcctgtt
     4681 ttcgttcacg caaaggcggc aggaacaacg ggaccaccga tagcctgtag tagaggtctt
     4741 cgcggaacgc gccttcggcc accgcctgtt tgagtttctg gttggtggcc gccaccacgc
     4801 gtacatccac cttgatagtg tcaaccccgc cgacgcgctc gaactccttc tcctgcaaca
     4861 cccggagcag cttggcct
//
LOCUS       DAOTAC010000196         4793 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_14716_length_4793_cov_7.429506, whole genome shotgun sequence.
ACCESSION   DAOTAC010000196 DAOTAC010000000
VERSION     DAOTAC010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4793
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14716_length_4793_cov_7.429506"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1078
                     /locus_tag="PLM74_08605"
     CDS             <1..1078
                     /locus_tag="PLM74_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015403577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HON43172.1"
                     /translation="AVWPSTGNYCRGGAYDSALLACQSIAILPEGMSRERFEWLAKVA
                     DETIATPGCESNVKEIFDKCHELTRERGDDIFIFNQFEEFGNYLWHYEITGHAIEEVL
                     AQVLGPHDTFFGITSNTGSAGTIGCGDYLKQRFPSSRIVASEALQCPTLLRNGFGGHR
                     IEGIGDKHVPWIHNVKNTDVVTAIDDEATLSLIRLFNEPAGRAYLIKQGVPESLVAQL
                     DLIGISGIANILSAIKAARWFELGHEDVIVTVLTDSMELYQSRLRELHEEHGEYTLTD
                     AAADYARYILGQSTDNMLELTYPERKRIHNLKYYTWVEQQGKTYTEIQQQWYQPDYWT
                     SFQGQVGEIDALITEFNARVGLLK"
     gene            1369..4491
                     /locus_tag="PLM74_08610"
     CDS             1369..4491
                     /locus_tag="PLM74_08610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HON43173.1"
                     /translation="MHKHESGILAILTVLALGALAARPMLGPSLPCSDDVGFHLLRAA
                     QLDLLLRQGILYSRWAPDMGLGYGYPLFNYLPQLPYYVVVLLGRLGGGLNVGVRLTFA
                     LSLWASGLAMYRFAREHFSAVAALVAAVAYMYAPYQAYDTLFRGNLSESFAWPLLPLA
                     LWGLGRLARHGGRKWVLLTALSYAAILLTHNIFAMITTPLLGLYVLVEAMEGRVFRVG
                     RDYIRRLVPPLGALALGMGLAAFSWVPAILERSVVQLDRLAFGFPFSYWNHFLSAGEL
                     FLSYCPVFLDLMNPSPPRTLGLLAVLCGLPGVLGVMWLKTGRQRRQVAFFGAALAGYA
                     FLMTALSRPVWDTFPLLQLTEFPWRLLSPAALCLALLAAGATDLIPRGKAQTVWGVLA
                     ISALIVGDLFWLTPRYCANPLHPGAADIRAQELAGLIGTTSAGEFLPVTVKALPATPA
                     DTFLDRESLPEGTDVFTETTTPLTGEVWLDLPQAAHLVFNRFAYPGWQARVDGISQPT
                     FPTTPEGLISLPVPAGAHLVEIAFGTTPLRVAATLVSLLSALALVLVERFGRRSRTEA
                     PVAASEALSARWLLFPVALVLLKTGLLDAVESPLSVHRLQGGGLQGVGVSTDLVFDHQ
                     LRLLGYEPFAVTTRADAPLALTLYVQDVVPGGPDYVIYPELLDAAGEVWIDFPYAPTV
                     YKPPPSQTSNWPPDQVAVLLFDLWPKIGTPPGRYTLALKVFDARSQFTYTAYAGDQAV
                     GPRVILGEIEITHPRTPFNVETLAPEAERMSALLGPVRLVWASIGAAEAHPGDPLKLQ
                     LAWETAASPQADLRAQLTLEAANGASFPLATLPLVHEDFPTTQWQVGDVWRGQHTLRL
                     PARLETGSYRLDLRLCALSGASCASMESTAAIGSLSLTAPERLWTVPPLDVSLDTRLG
                     ASVTLVGVQLTPYQMPLHAGEPFTVTLVWRDEAELDTALRVFVHLQDDSGYPVTQSDG
                     EPVQWSRPTTGWLPGEIVLDERTLSLPPDLAPGRYRLVAGLYAPEGPRLLTADGRDAV
                     LITELWVAEP"
BASE COUNT          780 a         1536 c         1451 g         1026 t
ORIGIN      
        1 ggccgtctgg ccgtccaccg gcaactactg tcgcggcggc gcgtacgact cggcgctgct
       61 ggcctgtcaa tccatcgcca tcctgccgga gggaatgagc cgcgaacgct ttgagtggct
      121 ggcaaaagtg gctgatgaaa ccatcgctac ccctggatgc gaatcgaacg tcaaggagat
      181 cttcgacaag tgtcacgagc tcacccgcga gcgcggcgat gacatcttca tcttcaatca
      241 gttcgaggag ttcggcaact atctctggca ctacgagatt accggtcacg ccatcgagga
      301 agtgctggcg caggtgttag ggcctcacga caccttcttt ggcatcacgt ctaacaccgg
      361 gtcggcgggc accatcggct gcggcgatta tctgaagcaa cggttcccct ccagccgcat
      421 cgtggccagc gaggcgctgc agtgccccac gctgttgcgc aacggctttg gcgggcaccg
      481 catcgagggc atcggcgaca agcatgtgcc gtggattcac aacgtcaaga acaccgacgt
      541 cgtcacggcc attgatgatg aggcgacgct cagcctcatc cgcctgttca atgagccggc
      601 gggccgcgcg tacctgatca agcaaggggt gccggagagc ctggtggcgc agctggattt
      661 gatcggcatt tccggcatcg ccaacattct cagcgccatc aaagcggctc gctggttcga
      721 gctggggcac gaggatgtca tcgtcacggt gctcaccgac tcgatggagc tctatcagag
      781 ccgcctgcgc gagctgcacg aggagcacgg cgagtacact ttgaccgatg ccgccgccga
      841 ttacgcgcgc tatatcctgg ggcagtccac cgacaacatg ctggaactga cttacccgga
      901 gcgcaagcgc atccacaatc tcaagtacta cacctgggtg gagcagcagg gcaagaccta
      961 taccgagatc cagcagcagt ggtatcagcc ggattactgg acgagcttcc aggggcaggt
     1021 gggcgagatt gacgcgctca tcacggagtt caacgcgcgc gtggggctgc tgaagtaata
     1081 tcccctggtt gcaaacatgc caggactccc tcgagatcct ggcatgtttg tatctcggtc
     1141 atcatgccga aaacacacca aaaacgcaga tccattagat tagaaggctt tccagggagg
     1201 atgtcaagcc ttcgcctttt ttgcgcttgg agcctgcctt gtgcgtctct gtctttcgcg
     1261 tccgacatgc ggcttctcaa gccgaggcaa ggcgcttttc ggttgctgaa cgtcgggggt
     1321 aaggtataat tttgccatca cgccgagggg cgatgagcgc ggtgggtcat gcacaaacac
     1381 gaatccggta tccttgcgat tctcacggtt ctggcattgg gggcgctggc ggcccggcct
     1441 atgttgggcc ccagcctccc ctgttccgat gacgtcggat tccacctctt gcgcgctgca
     1501 cagctcgatc tcctgttgcg ccaggggatt ctctacagcc gttgggcgcc tgatatgggc
     1561 cttggatacg gctatcccct gttcaactac ctgccccagc tcccgtacta cgtcgtcgtt
     1621 ctgctgggaa ggctgggcgg ggggctgaat gtcggagtgc gtctaacctt cgccttgagc
     1681 ctgtgggcct ccggactggc aatgtaccgg tttgcgcgcg agcacttcag cgcggttgcc
     1741 gccctcgttg ccgccgtcgc ctacatgtac gctccctacc aggcctacga caccctgttt
     1801 cgcggcaatc tctccgagtc ctttgcctgg cctctgctgc cgctggcgct ctgggggctt
     1861 ggccggctgg cccgccatgg cggtcggaag tgggtcctgc tcacggccct gagctatgcc
     1921 gcgattctgc tcacccacaa catcttcgcc atgatcacga cgccgctcct gggactgtac
     1981 gtgcttgtgg aggccatgga ggggcgggta ttccgcgttg gccgggacta cattcggcgg
     2041 ttggtcccgc cgctgggggc gctggctctg gggatgggac tggcagcctt ctcctgggtc
     2101 cccgcgattc tggagcggag tgttgtgcag ttggatcgtc tggcatttgg cttccccttc
     2161 tcatactgga accatttcct ctccgcgggt gagctgttcc tgtcatactg cccggtgttc
     2221 ctggacctca tgaatccatc gcccccgcgg acgttggggc tgctggccgt gctctgcggt
     2281 ctgcccggcg tgttgggggt gatgtggctg aaaacgggcc gccaacgacg tcaggtagcg
     2341 ttctttgggg cggctctcgc cggctatgct tttctgatga cggctctgtc gcggccggtg
     2401 tgggatacct tcccccttct tcagctgact gagttcccct ggcggctgct cagtccggcg
     2461 gcgctctgtc tggcgctcct cgccgcaggc gcgacagacc tcattccccg aggcaaagcg
     2521 cagacagttt ggggcgtcct cgctatatcg gccctgatcg tcggggacct tttctggctc
     2581 accccgcgat attgcgccaa tccgctgcac cctggcgccg ctgacattcg cgctcaagag
     2641 ctggccggac tcatcggcac gacatctgcg ggcgagttct tgcccgtgac cgtcaaagcg
     2701 ttgccggcga cgcccgccga tacattcctc gatcgcgaat ccctgcctga agggacggac
     2761 gtcttcaccg agactaccac cccgcttaca ggagaggtat ggcttgacct cccgcaggcg
     2821 gctcacctcg tcttcaatcg cttcgcctat cccggctggc aggcccgcgt ggatggcata
     2881 tctcagccga ccttccctac tacccccgag gggttgatct ccttgccggt accggcgggc
     2941 gcgcacctcg tcgagatcgc gttcgggaca acgcccttgc gggtggcggc gacgctcgtc
     3001 tcgctgttga gtgcgctggc gctcgtcctc gtcgaacgat tcgggcggcg cagcaggacc
     3061 gaagcgccgg tcgcagcgtc tgaggcgctc tccgcccgct ggctgctttt cccggtcgcg
     3121 ctcgtcctgt tgaaaaccgg gttactggat gccgttgagt cgccgttgag cgtccatcgc
     3181 ttgcagggag ggggcctgca aggcgtaggc gtttccaccg acctcgtctt cgatcaccaa
     3241 ttgcggctcc tgggatacga gccttttgcc gtgacgacac gggctgacgc tccccttgcc
     3301 ctgacgcttt acgttcagga tgtcgtcccc ggcgggccgg actatgtgat ctaccccgag
     3361 ctgctcgatg ctgccggcga agtgtggata gatttcccct acgccccaac cgtgtacaaa
     3421 ccgccgccgt cccagacctc caactggcct cccgatcaag tcgccgtctt gcttttcgac
     3481 ctgtggccca agatcgggac gccgccgggt cgctacacgc tcgcgctgaa agttttcgat
     3541 gcccgatcgc agttcaccta cacggcctac gccggcgacc aggctgtggg acctcgggtc
     3601 atcctgggcg agatcgaaat cacacacccc cgaacgccct tcaacgtcga gaccctcgcc
     3661 ccggaagccg agcggatgtc cgcgctgctt ggtccggtgc gcctggtgtg ggcttcgatc
     3721 ggcgcagcgg aggcgcatcc tggtgatccc ctgaagctgc agctggcctg ggagactgcg
     3781 gcctcaccgc aggctgacct ccgggcgcaa ctgacgcttg aagccgccaa cggggcctcc
     3841 ttcccattgg cgactctgcc cctcgtgcat gaggactttc ccacgacgca atggcaggtg
     3901 ggggacgttt ggcgtggcca gcacacgctg cggctgccgg cccgcctcga aaccggctcg
     3961 taccggctcg accttcggct ctgtgcgctt tccggagcgt catgcgcctc gatggaatcg
     4021 accgctgcca tcggcagcct gtctctgact gcaccggaga ggctgtggac ggttcccccc
     4081 ctcgatgtgt ctctcgatac gcgccttggc gctagcgtca ccctggtcgg agtgcagctc
     4141 acgccttatc aaatgcctct ccatgccgga gagccgttca cggtgaccct tgtctggcgg
     4201 gacgaggcgg agctggacac ggcgctgcgc gttttcgtgc atcttcagga cgactccggc
     4261 tatccggtca cccagtcgga tggcgaaccc gtacagtgga gccgtcccac tactggctgg
     4321 ctccccggcg agatcgtgct cgatgagcga acgttgtccc tgccccccga cctggcgccg
     4381 ggacgttacc gcctcgtcgc cgggctctac gcgcctgaag ggcctcgtct gctgacggcg
     4441 gacggacgcg atgccgtgct tatcacagag ctgtgggttg ctgagccctg aggcattcga
     4501 gcaatgggta agcataggat tctcaatttg gttctgggac actcctatca cccgtgcccg
     4561 aagggggtat agcctggcgt ggcgactttg agttgaagtt gtggcgtgat gtcatccagt
     4621 gatttgttcc ctcattgttc actaccccat cactaactga aaacagactt tatatggtca
     4681 gatggttaga ctttcctggt agaagcggtt acaaatcaaa cttgttttga ctaaccaacc
     4741 ggctcagcgc tttccatact cctttccata ctcgtgttat aatgcgagag tca
//
LOCUS       DAOTAC010000197         4780 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_14763_length_4780_cov_5.178201, whole genome shotgun sequence.
ACCESSION   DAOTAC010000197 DAOTAC010000000
VERSION     DAOTAC010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4780
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14763_length_4780_cov_5.178201"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(512..2185)
                     /locus_tag="PLM74_08615"
     CDS             complement(512..2185)
                     /locus_tag="PLM74_08615"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HON43174.1"
                     /translation="MTSVGSPHHPVLARMLCVGLVMLLVLAAITAGCSNDKPTVSRES
                     GKTIYDYCEKSDAAAKAVVKQLEVAGVLNPGAPPRAEAQRIIRRSTMGDAYLRWNLTT
                     YHAPSWFASADLLSYVVSRAVRKAGATVALPTDTSGALVFTIGFSDRRLKEMGESLDV
                     IRLVVPVGPAGEIAEAADPSWVIGDDTSASVPAKVGAGVVSPASENDSGVYQDLVGGE
                     KPGGSEEYVPREEPLAPTGPGQVSRARARLAIVIDDLGSGVAGTEQLFQVKAPLTVAV
                     LPDGRRARSEATTAAERGFAVLLHQPMEPHDPAKRPGGGAIMTGMSEDEIRRVLAANL
                     AAVPNVVGISNHMGSKITEDRDAMSTIMDETFSHGLFFFDSRTTPNSAAPRVARDMGA
                     PLLENARFLDHVADEAYVVDQIRAAARIALSRGAAAVIGHVRPTTVRALVRMIPELEA
                     AGVQLVRLDELMPAGWKSTIAKLQKPTEEPAQAPPQEATDSPAPAAAPATGQEAAVPT
                     QVQPVPGGEGQPTSVVPPIPSAGQDDHRGDQAEVQPEPTDAPPAADGNI"
     gene            complement(2195..3457)
                     /locus_tag="PLM74_08620"
     CDS             complement(2195..3457)
                     /locus_tag="PLM74_08620"
                     /inference="COORDINATES: protein motif:HMM:NF036497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HON43175.1"
                     /translation="MILLAVILRSIVGILSYPTTMILFWVVMAIVYSQYTRLAAMERQ
                     WTKGSPSPLVRTMRSLGEGILGGIAGSFLFVLLGPSIDRMGLGFLWAAALALAILDAR
                     LMCFSYAAGLACIGNLLLGWPKLDVASVTAMVGVLHLVEAGLIWATGSHSAIPVLISG
                     PDGRPAGGFLMERFWPVPVAVGLMLYYPLSAALPEVLAMPEWWPLIKSAAPAPEGMQS
                     VYALIGITAALGYSDLTYTRLPRQKARVTAAMSGCFSLILLGLSVLSSTRSWARWLAA
                     LFSPVGHELMVHLSRAAEIRGRPVFQAPAQGIMVLDIVRRSPAWQAGIRRGDVIESVN
                     CRPISSGDDLLEAMAVSPVTIVMDVRSAVPGGPPGARTVQIRGARSGLGLYLVPQEGK
                     YPDVRSTRAGLLWLIARYLRARSSGRRM"
     gene            complement(3454..>4780)
                     /locus_tag="PLM74_08625"
     CDS             complement(3454..>4780)
                     /locus_tag="PLM74_08625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HON43176.1"
                     /translation="KDRKRQGSGRFGWLAALGVLLALLVVFAGLAPRVDGVQSGGEAA
                     APPKFRDAKTALEAMYLVKGEFYKPVSLSTLIRAYLSEGTVSGMLAVLGDPYTRYMNP
                     KEFQTLKDDSAGVFGGVGILVGIRDEQITIISPIQNTPGMRAGLMPGDHILEIDGVST
                     DGMALDGAVSMMRGEPGTSVELVVERRYPAGRFTVEIVRDIIDAPATRGYMVDKSSGI
                     GYIELRAFSQKAGQDIESEVVSLLNQGMKGLILDLRYNGGGLVTQAVEVSSKFIPAGA
                     VMHVQGRDGQKKTLYTAGRRITDIPMVVLVNEYTASASEIVSGALQDTKVATVVGVPT
                     FGKGLVQTLFPLSDGSGLAVTTQIYLTAGGREITPDSPIKPDVEVQALTPEELERIKN
                     EQAKQGGPAGPVTAEDLLRIDPALDPQLRRALEILGDQIESSKTRRNAA"
BASE COUNT          951 a         1559 c         1336 g          934 t
ORIGIN      
        1 ggttttctta gtagatcaag ggctgcctga gggctttttc cggcgcgctc gagaaacccg
       61 cccgcgtatc catattatgt tacccgccta tgcctgcgga tttgttgccg gcgtgactcc
      121 gaaaaacagg ggcgccgttg ccggcgcgaa ttcagataac tcgtacctgc caaaccaccc
      181 agaatccgag gaggttgatc aaaaaaccta ccggaaaacg aataggatga gccagaatct
      241 ggttgagcca aatgcgcctg tatcggggac caggtaatcc cactctacgg caaatagaat
      301 gccccggaac ccccgttaga gcccacacag gagaacgccg tacccaggaa tgtccatgga
      361 tgtgggtggc gcatagcaga gaatccacag gtgacgggga acgaagtggt gacgacacga
      421 gagctgttga ggccgcggcc agatcgtccg gggagacagt gtgattcgtg gatccagctg
      481 gccatttgac atccgtacat atgatctaca tctagatgtt tccatcagcc gccggcggtg
      541 catcagtagg ttcgggctga acttctgcct gatcccctcg atgatcgtcc tgcccggcac
      601 tgggtatagg cggcactaca gatgtcggtt ggccctcgcc ccccggtacg ggctgcactt
      661 gggtgggcac cgccgcctcc tggcctgttg ccggcgctgc tgctggagcg gggctatccg
      721 ttgcctcctg gggcggcgcc tgagccggct cctcggttgg cttttgcagc ttcgctatgg
      781 tcgacttcca gcctgcgggc atcagctcat ctagcctcac aagctgcacg cctgcagcct
      841 ccagttccgg gatcatccta accaatgccc gaactgtggt ggggcgcaca tgtcctatta
      901 ccgctgccgc cccgcgcgat agcgcgatgc gcgctgcagc cctgatctga tctaccacat
      961 atgcttcatc tgcgacatga tcgaggaatc gcgcgttctc aagcaatggc gcgcccatgt
     1021 cgcgtgccac acgcggcgct gccgagttcg gagtagtcct gctatcgaag aagaacagcc
     1081 catggctgaa cgtctcgtcc atgatagtac tcatcgcgtc gcgatcttca gtgattttcg
     1141 accccatgtg attgctgatg ccgaccacgt taggcacggc tgccagattt gctgccaaga
     1201 cccgcctgat ctcatcctcg ctcatgccgg tcatgatcgc tccgcctccc ggacgtttgg
     1261 ccggatcgtg cggttccatt ggctggtgca gcagcaccgc gaacccacgc tcagccgcag
     1321 tcgtcgcctc gctcctagcg cgcctgccat cagggagcac ggccactgta agcggggcct
     1381 ttacctggaa caactgctca gtcccggcga cgccgctacc caggtcgtcg atgacaatgg
     1441 ccagccttgc gcgggcgcgc gaaacctgac ccggtccggt cggggccaaa ggttcctccc
     1501 tgggtacata ctcctcagac ccgcccggct tctcgccgcc caccaggtcc tgatacaccc
     1561 ctgaatcatt ctcactcgca ggggagacga cgccggcgcc tactttcgca ggaacgctcg
     1621 cgctggtgtc atcgccgatg acccacgacg gatcggcggc ctccgcgatc tcgcctgcag
     1681 gaccgacagg cactacaagc cgaatcacgt ccagcgattc acccatctct ttgagtctgc
     1741 ggtcgctgaa cccgattgtg aaaacaagag caccagatgt gtccgtcggc agagccactg
     1801 tcgcccctgc tttccgcacg gctcgcgata cgacgtacga cagcaagtcc gcactcgcga
     1861 accacgaagg ggcatggtat gtcgttaggt tccaccggag gtatgcgtcg cccatggttg
     1921 accggcgaat gatccgctgg gcttctgcgc gcggcggcgc gccaggattg agtacgcccg
     1981 cgacctccag ttgcttcacc acagccttgg ccgctgcgtc ggacttttcg caatagtcat
     2041 aaatcgtctt gccgctctca cgtgatactg tgggcttgtc attggagcac cctgcagtaa
     2101 tcgctgccag cacgagaagc atgactagcc ccacgcataa catacgcgca aggaccggat
     2161 gatgcggtga cccaacgctg gtcatggttg acgttcacat cctccgccca ctcgagcggg
     2221 ccctgagata tcgcgctatg agccacagaa ggccggcgcg cgtcgaacgt acgtcgggat
     2281 atttcccctc ctgcggtacc aggtagagcc ccaaccccga tcgggcgccc ctaatctgga
     2341 cagttcttgc cccgggcggc ccgcccggaa cagccgagcg cacatccatc actatcgtaa
     2401 cgggcgatac ggccatggcc tccaataggt catcgccgga ggaaatgggc ctgcagttga
     2461 ctgactcaat gacgtcgccg cgccttatgc ctgcctgcca ggcaggtgac cgccggacaa
     2521 tatccagaac catgatgcct tgggcaggcg cctggaacac gggccgtccg cgtatctcgg
     2581 ctgctctcga aagatgaacc atcagctcat ggccgaccgg tgagaacagc gcagcaagcc
     2641 agcgcgccca gctgcgtgtt gacgaaagta cggacaggcc cagcaggatc aggctgaaac
     2701 atccagacat ggctgcggtc acacgagcct tctgtcgggg aagccgggta taggtcaaat
     2761 cgctgtagcc cagggcagcg gttatgccta tcagtgcata caccgactgc atcccctccg
     2821 gggcaggggc agccgacttt atcagcggcc accactcggg cattgcgagg acctcgggga
     2881 gggcggcaga cagcgggtag tagagcatta ggccgacggc cactggcacc ggccagaacc
     2941 tctccattag gaacccgccc gcaggcctgc cgtcgggccc cgagatcagc acggggatgg
     3001 cgctgtgact gcccgtcgcc cagatcagac cggcctccac gagatggagc actcccacca
     3061 tggcggtgac agatgccaca tccagcttgg gccaacctag caacaggtta ccgatgcacg
     3121 cgagccctgc ggcatacgaa aaacacatca gacgcgcatc cagtattgcc agcgccaatg
     3181 ccgccgccca tagaaagccc aggcccatcc ggtctatgga tgggccaagc aacacgaaga
     3241 gaaagctacc cgctattccg cccagtatac cttcgcctaa cgacctcatg gttcggacaa
     3301 gcggcgatgg cgagcctttg gtccactgcc gttccatcgc cgcaagccgc gtgtactggg
     3361 aatatacgat ggccatcacg acccagaata ggatcatcgt agtggggtac gagagtatgc
     3421 ctacgatgct cctgagtatg acggccagaa ggatcatgct gcgttccgcc tggtcttgct
     3481 cgactcgatc tggtctccca gaatctccag cgctcgtcgg agctgcgggt cgagagctgg
     3541 gtctatcctg agcagatcct cggcggttac gggcccggcc ggaccgccct gtttcgcctg
     3601 ctcgttcttg attctttcca gttcctcagg ggtgagcgcc tgcacctcta cgtccggctt
     3661 gatgggcgaa tccggagtga tttctctacc cccggcggtc aagtatatct gcgtggtcac
     3721 tgcgagcccc gacccatccg acagcgggaa gagcgtctgc acaagtccct ttccaaacgt
     3781 gggaacgccc accacagtag cgactttcgt gtcctgcaat gcccccgaca ctatctccga
     3841 tgccgaagcc gtatactcgt tcaccagcac caccatgggt atgtcggtta tgcggcgacc
     3901 agctgtgtat agcgtcttct tctggccatc tctgccctga acgtgcatga cggcacccgc
     3961 ggggatgaac ttgctggaga cctccactgc ctgcgtaacc aggcctccgc cattgtaccg
     4021 gaggtcgaga atgagccctt tcatgccctg attcagcagg ctgacgactt cactctcaat
     4081 atcttggccg gctttctgtg agaatgccct cagttcgata tagccgatac ccgaggactt
     4141 atcgaccatg tatcccctgg tggcaggggc atcgataata tcgcgcacta tctccacagt
     4201 aaaacgcccg gcagggtaac gtcgttcgac aacgagctct accgaagtgc caggctcgcc
     4261 cctcatcatg gatacggctc cgtccagcgc cattccgtcg gtcgatacgc cgtctatctc
     4321 caggatgtgg tcccccggca tcaggccggc cctcattccc ggcgtgttct gaatcggcga
     4381 gatgatcgtg atctgctcat cgcgtatacc caccaagatg ccgactcccc cgaacactcc
     4441 tgccgaatca tccttgagcg tctgaaactc cttcgggttc atgtaccgcg tgtatggatc
     4501 tcccaggacc gccaacatac ctgagacagt gccctctgac aggtatgctc gaatcagtgt
     4561 cgataaggac acgggcttgt agaactctcc cttgacaagg tacatggcct ccagagccgt
     4621 cttggcgtcc ctgaacttgg gcggagccgc agcctcgcct cccgactgga ctccgtcgac
     4681 cctgggcgct aagcccgcga acacaactag cagcgcgagc agcacaccca atgcggcaag
     4741 ccaaccgaac cttcccgagc cctgcctttt tctatcttta
//
LOCUS       DAOTAC010000198         4776 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_14783_length_4776_cov_3.997035, whole genome shotgun sequence.
ACCESSION   DAOTAC010000198 DAOTAC010000000
VERSION     DAOTAC010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4776
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14783_length_4776_cov_3.997035"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1102)
                     /locus_tag="PLM74_08630"
     CDS             complement(<1..1102)
                     /locus_tag="PLM74_08630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endospore germination permease"
                     /protein_id="HON43177.1"
                     /translation="MKMAEAREIIANRQLAAILFMMRTTVVISFVPLLTTGNAAQDAW
                     AAGLIAGLLLTLAAWGIAELAARYPRLTVVDYSRMLLGRFLGTIVSLVISWHFLLIAA
                     TDIRIYAELMRVAFMPNTPIWFTIVAMAFLASLAAWFGIEPIGRAADAFLPIFAAFVA
                     LTLLGAVPSFNVHNLQPALARGLRPVLSSVWTPLGIGLQWISAGMLFPFLIPQNRRVH
                     SVVLAAVLPSAVLAVVAAMLVGVLGTDLGSKSLFPFLKMARSVRPTRTIERIEILGTV
                     AWGLGLFAGGSTMVYCGSRALSQILRITEYRKIVPVFGVLVALYSMYAYKDAFQLVRF
                     FRPPGFPLLIALTAGVPYALMWASHIARHLLRR"
     gene            complement(1099..>1357)
                     /locus_tag="PLM74_08635"
     CDS             complement(1099..>1357)
                     /locus_tag="PLM74_08635"
                     /inference="COORDINATES: protein motif:HMM:NF015292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HON43178.1"
                     /translation="VLILASRRSFGHPYLAPVAPFIGGGWKDYLFRTWHWAKTMRPKL
                     TGGREPIRAPGGQMPRPGASPESEGLQQLQPEGGGAKSKGR"
     assembly_gap    1358..1457
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1458..2972)
                     /locus_tag="PLM74_08640"
     CDS             complement(<1458..2972)
                     /locus_tag="PLM74_08640"
                     /inference="COORDINATES: protein motif:HMM:NF015292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HON43179.1"
                     /translation="MAFNLVRALISRFARRTRRGGFGASPGRSSPSSGRKRIDPELLA
                     DDSLDVGARKAVTDRLALDARLAENLRMLELAAGPSPDIIVRRIATDASLDIAVINLQ
                     GIVDQERIEGVVRAVSSAGVASAPELISELGAHLLRSSHVKTVSSFTDLWDALSGGLA
                     VVLADGHNQGLACSAQSFKTRPIQEPPTDTTLRGPRQGFIEDLTTNLAILRQWIRTPN
                     LWIEETRLGHLTQTRLAIAYVKGLASEELLAEVRQRIQRIETDSVLGTGPVEDFIQDT
                     HLSFFPLILRTERPDRVVGNLLEGRVAIMVQNSPFALVAPMDYGMLFQSADDYYEPVP
                     IVFVISVLRVIAFWTSILLPGAFVAVLTFHHELLPTSLLLRIVGDRAGVPFPVIVEVF
                     LMELTFELLREAGLRLPRAVGSAVTIVGALVLGEAAINAGLVSPSVIIVVATTAIASF
                     ILPTFSFDISARLLRFAFIVAGGSIGLFGVQYGIIMVVLILASRRSFGHPYLAPV"
     gene            complement(3090..4130)
                     /locus_tag="PLM74_08645"
     CDS             complement(3090..4130)
                     /locus_tag="PLM74_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43180.1"
                     /translation="MIVRPAEPRDDEVLLRIESMAGQGDRIRLIDERDSFFSRARQFN
                     NTILLVGENEATGALHGVVAAAVQRLIVAGQEHLGAYMFDLRSNPEANRGLSRAMFFL
                     WKGLEERLMDQGVEFIFGLIKEDNSRSYSIATRMGAEARGEKRFYTIPTFRRKRPLQE
                     VKAARRIDARQEYTDLSTHYADFSLLPIVDDFDHAQSLYDHYLVARLECGESSLKLWD
                     VSRHYTRRVAAMPRAYELAGSMLRGLARLVPLPKIPAPGEAINVWNVLDLVTRRGREE
                     EVRSLLAQANNMAVDHGIDYLIVAVDEEQAELAWLRHMAVMSLNYKVMVKEYRPVPAL
                     VGKTYLDPRWLG"
     gene            complement(4184..>4776)
                     /locus_tag="PLM74_08650"
     CDS             complement(4184..>4776)
                     /locus_tag="PLM74_08650"
                     /inference="COORDINATES: protein motif:HMM:NF015197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HON43181.1"
                     /translation="MSQSIVPEVVRGLAHDPKSIVRAIKWGLGINLLFLLLFPVAIFG
                     LQNPDAISQVVTISWGHTLGPAVYLTVNLFALLALLTSFWASAGAVMGNVVEIMRVES
                     EWNIRARLVAFGVTVAPSLALVFRGRLEFGDMIEAAGAVGGVILAVLPIFVLARARAA
                     SGRRPEYQVGILYSPIVRIALFVFYAGVLAYAAFAR"
BASE COUNT          940 a         1378 c         1468 g          890 t
ORIGIN      
        1 ggcggcgcaa caggtgtctt gctatgtgtg aggcccacat gagcgcgtac ggaacgccgg
       61 cggtgagcgc gatgagcaga gggaagccgg gcgggcggaa gaacctgacg agctggaagg
      121 cgtctttgta cgcgtacatc gagtaaagcg caaccagcac tccgaagaca ggcacgatct
      181 ttcggtactc tgttatcctt aggatttgcg acaacgctcg gcttccgcag tacaccatgg
      241 tggaacctcc cgcaaacagc cctaaccccc atgcaaccgt gcccaggatc tcaatacgct
      301 cgatggtgcg ggtgggcctg accgaccgcg ccatcttgag gaatggaaat agcgacttcg
      361 agccgagatc ggttccgagt acgcccacaa gcattgctgc aactaccgca agcactgcgc
      421 tgggtagaac tgccgctagg actacgctgt gcaccctgcg attttgcggt atgagaaagg
      481 ggaaaagcat ccctgccgat atccactgaa ggccgatgcc cagcggtgtc catacgctgg
      541 acaagactgg acgaaggcct cgtgcaagcg ccggctggag gttgtggacg ttgaagctgg
      601 gcacggctcc aagcaatgtg agtgcgacaa acgccgcgaa gatgggcagg aatgcgtcgg
      661 cggcgcgccc gattggctcg ataccgaacc atgctgccag ggaggccagg aaggccatgg
      721 cgacgatggt gaaccagatg ggagtgttgg gcatgaacgc cacccgcatc agctccgcgt
      781 agatgcggat gtcggttgcg gcaatgagca ggaagtgcca ggagataacg agcgagacga
      841 tggttccgag gaaccgcccg agcaacatgc gcgagtaatc cacgacggtg agccgcggat
      901 accttgctgc cagctcggca ataccccaag ccgcgagtgt gagcagcagc cctgcgatca
      961 gcccggcggc ccacgcatcc tgggcggcgt tgccggtggt gagcagcgga acgaaggata
     1021 tcaccacagt ggtccgcatc atgaagagta tggctgcaag ctgccggtta gctattattt
     1081 cccttgcctc ggccatcttc atcgtccttt gctcttggcg ccgccgccct caggctgcag
     1141 ctgctgcaac ccttcgcttt cggggcttgc gcccgggcgc ggcatctgtc ctccgggcgc
     1201 gcgtataggt tcgcgtcctc ctgtaagctt cggccgcatc gtcttggccc aatgccaagt
     1261 gcggaatagg tagtccttcc agcctccgcc gataaacggg gcgaccggcg caaggtatgg
     1321 atgcccgaaa ctgcgtcggc tggctaggat cagcacannn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnngac cggcgcaagg tatggatgcc cgaaactgcg tcggctggct
     1501 aggatcagca caaccatgat gatgccgtat tgcaccccga ataggccgat gctgcctccg
     1561 gccacgatga acgcaaatcg cagtagccgt gcggatatgt cgaaggagaa cgtgggtagg
     1621 atgaacgagg ctattgccgt ggtagccact acgattatca ccgatggcga caccaggccg
     1681 gcattgatgg cggcctcgcc gagcacgagc gctccgacta tggtgacggc cgaccccact
     1741 gcgcgcggta gcctaagccc cgcttcgcgc agcagttcga acgtcagttc catcaggaag
     1801 acttctacga ttaccgggaa tggcacgcca gcccgatcgc ccacgatcct gagcagcagt
     1861 gaggttggaa ggagttcgtg gtggaaggtg agaaccgcga cgaacgctcc tggaaggagt
     1921 atggaggtcc agaatgcgat tacccgcagc actgagatga cgaacactat aggtaccggc
     1981 tcgtagtagt cgtcggcaga ctggaagagc atgccgtagt ccatgggcgc caccagcgcg
     2041 aacggggagt tctgtaccat gatcgcgacg cgcccctcga gcaggttgcc gacgactctg
     2101 tcaggtcgtt cggtgcgcag gatcagcggg aagaacgaca gatgtgtgtc ctggatgaag
     2161 tcctcaacag gcccggtacc cagcaccgag tcggtttcga tacgctggat acgctggcgc
     2221 acttcggcca ggagttcctc gcttgccagg cctttgacat aggcgattgc gagtctggtt
     2281 tgtgtgagat gccccagtct ggtctcctca atccacaggt tcggggtgcg gatccattgc
     2341 cggagaatgg cgaggttggt tgtgaggtct tctatgaatc cctgccgcgg cccgcggagg
     2401 gtcgtgtcgg tggggggttc ttggattggt cgagtcttga aagactgggc gctgcaggcc
     2461 agtccctgat tatggccatc tgctaggacc actgcgagcc cgccgctgag cgcgtcccat
     2521 aggtcggtga aggacgagac tgttttgacg tgcgaagaac gtagcaagtg ggcgccgagt
     2581 tccgagatca gttcgggcgc cgaggccacg ccggccgacg acacggcacg cacgacgcct
     2641 tcgatgcgct cctgatcaac gatgccttga aggttgatga cggcgatatc cagcgaggca
     2701 tcggtagcga tccgcctgac tatgatgtcc ggcgacggcc cggcagccaa ctcaagcatt
     2761 cgcaggtttt cagccagcct ggcgtcgagc gcgagcctgt cggtgacggc tttgcgtgcc
     2821 cccacatcca gagagtcgtc ggcgagcagc tccgggtcga tccgttttcg cccggaggag
     2881 ggggatgaac ggcccggcga cgcgccgaaa ccgccgcgtc tcgtgcgtct tgcaaagcgg
     2941 gatatgagtg cccggacgag gttgaacgcc aagctgagcc cgtctccttc catggctgcg
     3001 ctgatcgtgc ttcagcttag cgtctccaat ggagtcaggc tgctattcgc gcaatgccta
     3061 tcaatgccta accgggcggc gttagggcgc tatcccaacc acctggggtc gagataggtc
     3121 tttccgacga gcgccggcac tggccggtac tccttgacca ttaccttata gttcagggac
     3181 attaccgcca tatgcctgag ccaggccagc tcggcttgtt cttcgtctac ggcgacgata
     3241 aggtagtcga tgccgtggtc cacggccatg ttgttggcct gggcaagcag cgaccttacc
     3301 tcttcttcgc gacctcggcg agtcacaagg tccagcacat tccatacatt gatagcctcg
     3361 cctggagcgg gtatcttcgg cagagggacg aggcgcgcaa ggccgcgcaa catcgaacct
     3421 gccaactcat atgcacgcgg catggcggct actcgtctgg tgtagtggcg ggagacgtcc
     3481 cagagcttga gcgacgactc tccacattca agccgggcca ccagatagtg atcgtagaga
     3541 ctctgcgcgt gatcgaagtc gtctacaatg ggtagcagac tgaagtcggc atagtgtgtg
     3601 ctgaggtctg tgtactcctg acgggcatcg atcctgcgtg cggccttgac ttcctgcagt
     3661 ggtctcttgc ggcggaaagt cgggatcgtg taaaagcgct tctcgccgcg ggcctcggcc
     3721 cccattcgag tggcaatgct gtaggaacgg ctattgtcct ccttgatcag gccgaagatg
     3781 aattcgacgc cctggtccat caggcgttct tcgaggccct tccagaggaa gaacatggct
     3841 ctggacaacc ctctgttggc ctcagggttg gagcgcaggt cgaacatgta ggcgcccaga
     3901 tgctcctgcc ctgccacgat caggcgctga acggctgctg ccacaactcc gtgcagcgcg
     3961 cctgttgctt cgttctcgcc taccaggagg atggtgttgt tgaactgacg cgcgcggctg
     4021 aagaagctat cgcgctcgtc gatcagacgt attcggtcgc cctgccctgc catggattct
     4081 atccggagca gcacttcatc gtctcttggc tcggcgggcc ttactatcac gctgggcccc
     4141 tcccttcatc tcattgcgcc ctcgattgct ccggccatgg ccgttatctg gcaaaagccg
     4201 cataggcaag tactcctgcg tagaacacaa acagtgcgat tctgacgatt ggggagtaaa
     4261 gtatgcctac ttgatactcc ggtcgcctgc ctgacgcggc tctggcacgg gcgagcacga
     4321 agatgggaag cacggccaga atcacccctc cgacggcgcc tgccgcctca atcatgtcgc
     4381 cgaattccag cctgcctcgg aagaccagcg cgagcgacgg ggcaactgtg actccgaaag
     4441 cgaccagcct ggcgcggata ttccactcgg attcgacccg catgatctca accacgttgc
     4501 ccatgacggc accggcactg gcccagaagg acgtcaacag cgccagcagg gcgaacaggt
     4561 ttactgtgag atacaccgct ggcccaagcg tgtggcccca cgagatggtc actacttgtg
     4621 aaatggcgtc agggttctgc aggccgaata tggccacagg gaacaggagc aggaagagga
     4681 ggttgatgcc gagcccccat ttgatggccc gcacgatcga tttcgggtcg tgggccagcc
     4741 cgcgcactac ctcaggcacg atggactgcg acatgg
//
LOCUS       DAOTAC010000199         4771 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_14803_length_4771_cov_5.081001, whole genome shotgun sequence.
ACCESSION   DAOTAC010000199 DAOTAC010000000
VERSION     DAOTAC010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4771
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14803_length_4771_cov_5.081001"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(250..957)
                     /locus_tag="PLM74_08655"
     CDS             complement(250..957)
                     /locus_tag="PLM74_08655"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HON43182.1"
                     /translation="MKEKKEKIVGIIGGMGPDATVDLFQKILRATPAKRDQDHLRIII
                     DCNSKIPDRTAHIMRGEENPGPCLVSSARLLEQAGVDVMAMPCNAAHAWHDEIQASVG
                     VPVLHIMRAAADYAAERWPNMKKIGLLAVPSTVKIGIYHRTFAEKGIEVIAPDQKHQD
                     IITEAIFAVKAGQTGPNIQRLAANAGEYLVAQGAEAVIAGCTEVPLILKDGDISVPVV
                     DATQALAVAVVKFARGM"
     gene            complement(1175..2398)
                     /locus_tag="PLM74_08660"
     CDS             complement(1175..2398)
                     /locus_tag="PLM74_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HON43183.1"
                     /translation="MKLWVKILIGFVLGAIVGLIVGPPIAVIKPLGDLFIRLIKMLIV
                     PMVFCSLVVGASSIGDLKKLGRVGGKTLAYFLVTTAFAITLAILLSLVIDPSAGYVIG
                     EVGTYEPKPMPSIVDTFLNMVPTNPISAAASDNILQLIVFALFMGIAISLLGEKGKPA
                     LAFFDSVAEIMYKITAIVMGFAPIGVFALIAWVTGTSGSAVLLPLAKLIFVVYLANIL
                     HAVIVYSGAVYIWAKINPMTFFKGFFEAMAVAFSSCSSSATLPISMRCAQDNLGVSKE
                     ISSFVQPLGATINMDGTAIYQGVCAIFIARVYGIPLGLPQYLMIILTATLASVGTAGV
                     PGAGMIMLSMVLQTVGLPLDGIMLVAGIDRILDMIRTCMNVTGDAAGAIVIAASEGEL
                     ARVAPAGGRGAAIAK"
     gene            complement(2523..2948)
                     /locus_tag="PLM74_08665"
     CDS             complement(2523..2948)
                     /locus_tag="PLM74_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43184.1"
                     /translation="MWDELAVKALEILACIGALFVLVLLHEASKYLREQARRVQNCLA
                     RELLMSAIAEAELVGVDAVYATNQVFVDALRRTSADGKLSKEDATNAMQIAVDYFRTH
                     ISSTSARILGSPAELADKWIREFLEAKLAQMKVDAASPR"
     gene            complement(3091..3726)
                     /gene="pcp"
                     /locus_tag="PLM74_08670"
     CDS             complement(3091..3726)
                     /gene="pcp"
                     /locus_tag="PLM74_08670"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HON43185.1"
                     /translation="MAKILITGFDAFGGESINPASGALQHLEHSRFSEGLAVEQGVEV
                     ITREIPTVFGASAEAVREAIQDIMPQAVISIGQAGGAHGIRVERVALNIDDARIDDNA
                     GNRPVDSRIEPEGPVAYWATIPVREVVEAIRKEGIPAAFSYSAGTFVCNHVFYSTCHF
                     IASRSLNVQVGFIHVPYLPEQVVEKDTAPSMSQGCIVTALEAALRAVAATL"
     gene            complement(3711..4703)
                     /locus_tag="PLM74_08675"
     CDS             complement(3711..4703)
                     /locus_tag="PLM74_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010296099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HON43186.1"
                     /translation="MRIEVEWHRRPQAGGGGVIMNSRWVVKPDVQKALDGGQPVIAFE
                     STVLTHGLPYPDSTKLALRVEEIACEEGCVPATIGVIDGVVHVGMTPDEIDRLAADKR
                     ACKSSAFDLAFVIARGASAGTTVSGTLAIAERAGISVFATGGIGGVHRGAGDSFDISH
                     DLQSLARSRVVTVCAGAKAILDLPKTLEYLETAGVCVIGYQTSSFPAFYSCDSGLSLD
                     YSAESADIIARAFMAREQLGLPGGILVAAPIPEEYEIPADQISTSIDLAVEQAAECGV
                     HGKEVTPFLLASLARITAGRSVEANLALLENNARVGARIARAISTEEVRSWRRY"
BASE COUNT          990 a         1482 c         1297 g         1002 t
ORIGIN      
        1 ccgcacctgc atgaacgtca caggcgacgc agcaggcgca atcgtgatcg cagcgaccga
       61 aggcgaacta gcgacagtca agcccgcacg cgggcgtgga gcggcggtcg caaggtaaca
      121 cagggcagga agcaatcgga gcaacaagca taagcataaa aggcgagctt ggcatctcgc
      181 gcactgcgag agccggctcg cctttcatgt gtcgccacgg taccaacgta gttgggatct
      241 gcccccaacc tacatcccgc gggcaaactt cactaccgca accgccaggg cctgggtcgc
      301 atccaccacc ggcaccgata tgtcgccgtc cttcaggatt agcgggacct cggtgcagcc
      361 ggcgatcacc gcctccgcgc cctgagccac cagatactcg ccggcgttgg ccgcgagcct
      421 ctggatattg ggtccggtct gccccgcctt gaccgcgaag atcgcttccg tgattatgtc
      481 ctggtgcttc tggtcagggg ctatgacctc tatgcccttc tcggcaaagg tccggtgata
      541 aatgccgatt ttcaccgtgg acggcacagc tagaagcccg atcttcttca tgttgggcca
      601 gcgctcggcc gcgtagtcgg cggcggcccg catgatgtgg agcacgggaa ccccaacact
      661 tgcctggatc tcgtcgtgcc aggcatgagc cgcgttgcaa ggcatagcca ttacgtctac
      721 ccctgcctgc tccaggagcc gagccgaact cacaaggcaa ggccccgggt tttcctcgcc
      781 cctcatgatg tgagccgtcc ggtcggggat cttggagttg cagtcgatga ttatcctcag
      841 atggtcctgg tctctcttcg ccggcgtggc ccggagtatc ttctgaaata ggtctaccgt
      901 ggcgtccggc cccatgcctc cgattatgcc tacgatcttc tccttcttct ccttcatatt
      961 cgcacatcgc ctcctataag gactccagtc cctcgtcgat cagccccttg tcaattagtc
     1021 agcactcttc ttctgtgatt gtacaacata gagtggtgtg cgcgcatcta tggcagagag
     1081 ccgtcaaatc ggaatgtgca tccgatattg ccggctctct tctgattcgg tcatgattcc
     1141 agtgtcatgc ccaatttggc gcatggctcc ctatctactt ggcaatagcc gctccccgtc
     1201 cgccggcagg cgcgactcgt gccagttcac cttcggacgc tgcgatcacg attgcgcctg
     1261 ctgcgtcgcc tgtgacgttc atgcaggtgc ggatcatgtc gagaatgcgg tcgataccgg
     1321 cgaccagcat gatgccgtca agcggtagcc ccacggtctg caggaccatg ctcagcatga
     1381 tcatcccggc gcccggcact cccgccgttc cgactgacgc cagagtggcc gtgaggatga
     1441 tcatcaggta ctggggcagt cccagcggaa taccgtatac ccgggcgatg aatatggcac
     1501 acacgccctg gtagatggcg gtgccgtcca tgttgatcgt cgctcccagc ggctgcacga
     1561 aactggagat ctccttggac acgcccaggt tatcctgagc gcaccgcatc gagatcggca
     1621 gcgtagcgct gctggagcag gagctgaagg ccaccgccat ggcctcgaag aatcccttga
     1681 agaaggtcat aggattaatc ttcgcccaga tgtacactgc tccggagtac acaatgacgg
     1741 cgtgaaggat attagcgaga tacaccacga agatcagttt ggccagtggc aatagcactg
     1801 ccgaaccact agtgcctgtg acccatgcga tcagcgcaaa cacgcctatg ggagcgaagc
     1861 ccataacgat ggcggtgatc ttgtacataa tctctgccac gctatcgaag aacgccagcg
     1921 caggcttgcc cttctcgccc aggagcgata tggctatccc catgaacagc gcgaacacaa
     1981 tgagttgcag gatattatca cttgctgcag cgcttatggg gttagtaggc accatgttaa
     2041 ggaacgtatc gactatgctc ggcatcggtt tgggttcata tgttcccact tcgccgatta
     2101 catagcccgc gctgggatcg atcaccaagg atagcaggat cgcaagggtt atcgcaaacg
     2161 cagtagtgac caagaagtac gccaaagtct tgccgcccac gcggccaagt ttcttgaggt
     2221 cgccgatgct acttgcgcca actaccagcg agcagaatac cattggcaca atcagcatct
     2281 tgatgagcct gatgaataag tctcccagcg gttttatcac cgctatggga ggtccgacaa
     2341 tgaggccgac gatcgcgccc aggacgaagc cgatcagaat tttgacccag agtttcatcc
     2401 ggtacgtacc ctccctctcg cttgattgga tcctaccgac ccattcaacc attgcagcga
     2461 aaaacccttc ttttcatcga actccgtagg ctggtatagc ctggtgggat atgcagtcgc
     2521 ggtcatctcg gagaggccgc atcaaccttc atctgtgcca gcttcgcttc gagaaactcg
     2581 cggatccact tgtcggcgag ttcggcaggg ctgccgagta tacgagcact cgtagacgag
     2641 atatgggttc tgaagtagtc cacagctatc tgcatggcat tcgttgcgtc ttccttcgac
     2701 agcttgccat ctgcggaagt gcgcctaaga gcgtccacaa acacctggtt cgtggcgtag
     2761 accgcatcaa cgcccacgag ctcggcttca gcgattgcgg acatgagcag ttcccgggcc
     2821 aggcaattct gtacccgcct ggcctgttcg cgcaggtact tcgatgcttc atgcagcaag
     2881 accagcacaa acagcgcgcc gatgcacgcc aggatctcca gcgccttgac tgccagttcg
     2941 tcccacatgt tcagcacctc ccaatatcag cgcagcggac gccgggcgcc gccgagcgcg
     3001 tgccccgtct cattctgcta attcttgctt tcgatatggg gatgctaaat ccttccgtaa
     3061 atggattggg aaacctacgg tgcgaacatg tcacagtgtt gcagctacag cccttaatgc
     3121 cgcctccagc gccgtgacta tgcatccctg cgacatgctg ggagcggtat ctttctcgac
     3181 gacttgttct ggtaggtacg gcacatggat gaaaccgacc tggacattga ggctgcgact
     3241 ggctatgaaa tggcaggtcg agtagaagac gtggttacat acgaaagtcc ccgccgaata
     3301 agaaaacgcc gcagggatcc cctctttccg tatggcttcc actacttcgc gcacaggtat
     3361 cgttgcccag taggcaacgg ggccttcagg ctcaatgcgc gaatcaacag ggcgattgcc
     3421 cgcattatcg tcgatcctgg catcgtcgat gttgagagcc acccgttcta cgcgtatccc
     3481 gtgcgcccct cccgcctgcc ctatgctgat gactgcctga ggcatgatat cctgaatcgc
     3541 ctctcgcacg gcctccgccg atgcgccaaa tacagtcgga atctcgcgcg tgatcacttc
     3601 cacgccctgc tctaccgcta gtccctcaga aaaccgtgag tgttccaggt gctgcagtgc
     3661 tccggaggcc gggttgatgg actcgcctcc aaatgcatcg aatccagtga tcagtatctt
     3721 cgccatgagc ggacctcctc ggtactgatt gccctggcga ttctggctcc gactctcgcg
     3781 ttgttctcga gcagcgccag gttggcttct actgaacggc ccgcggtaat cctcgctagg
     3841 ctggccagga ggaacggagt cacttccttg ccgtgaacgc cgcattcagc ggcctgctcg
     3901 acggcaaggt cgatcgaagt actgatctgg tcagccggga tttcgtattc ctctggaatc
     3961 ggcgccgcga ccagaatccc tcctgggagc ccaagttgct cgcgggccat gaaagcccgc
     4021 gcgatgatgt ctgccgactc tgcggagtag tcgagcgaca acccggaatc gcacgagtaa
     4081 aacgcaggaa atgaactagt ttggtatcca attacgcaca cccccgcggt ctccaggtac
     4141 tcgagggtct tgggcagatc cagtatggcc ttggccccgg cacacaccgt aacgacacgc
     4201 gaacgggcca ggctctgtag atcgtgggag atgtcgaagc tatccccggc tccgcgatga
     4261 acgccgccta tacccccggt tgcaaagaca cttatccccg ctcgctctgc aatggccagc
     4321 gtcccggaga ctgtggtccc tgcagacgcg ccccgggcaa tcacgaaggc cagatcgaac
     4381 gcggaggact tgcaggcccg cttgtcggca gcgaggcgat ctatttcgtc cggagtcatt
     4441 cccacatgaa caaccccgtc gatgacgccg atggtcgcag gaacacaccc ctcctcgcac
     4501 gcgatctcct ctacacgcag agccagcttc gtgctgtcgg gataggggag cccatgagtc
     4561 agaaccgtag actcgaatgc gattaccggc tggccaccgt caagcgcttt ctgcacatcc
     4621 ggtttgacta cccaacgact attcatgata acaccgcccc cgcctgcctg tggtcttctg
     4681 tgccactcca cttctatgcg caattgagaa atcctccggc gaatctgccg gtcgccgtat
     4741 cggacactat attcgaatat tgaacaaagc g
//
LOCUS       DAOTAC010000200         4758 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_14843_length_4758_cov_4.625771, whole genome shotgun sequence.
ACCESSION   DAOTAC010000200 DAOTAC010000000
VERSION     DAOTAC010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4758
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14843_length_4758_cov_4.625771"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..878)
                     /locus_tag="PLM74_08680"
     CDS             complement(156..878)
                     /locus_tag="PLM74_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016205794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HON43187.1"
                     /translation="MLAQNYLNRVRRILDEIEKTQLEAIGRAADIVTAAIRGGGRLYL
                     FGTGHSHIIAEEAFYRAGGLVPVNAILEPALMLHEGPFKSTAMERLEGYAQIILDHSG
                     IGPGDALMIISNSGRNAVPVEMALIAKARRIPVIVLTSLAHSRSVPSRHSSGKHLFDV
                     ADVVIDNCGVPGDAVLEADGSAVQICPTSTVAGAAIINMIEAEVVERLCAMGVEPPVF
                     VSANIDGGDEFDQQWKGVLCRR"
     gene            complement(872..2392)
                     /locus_tag="PLM74_08685"
     CDS             complement(872..2392)
                     /locus_tag="PLM74_08685"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HON43188.1"
                     /translation="MFNVGSGLGRIQLPPSIPLAGYQARKEVSRGVHDPLYARALAVE
                     FDGRCIAILSLDILYSDVRLTRMIRQSATDAVPAGAASGSASVMVCCSHTHSGPSGIF
                     PDSGSFDPPTIDAILSAAAEAMAQAWAELLPCRLYLGSGQVHGMASRRSDLAGHGATG
                     PMGQRIAVAEFRSLDQGSSGAPRATMRLVSLACHPTVLGPDNPLVSRDWPGFMVDACE
                     HEMRTSGAERAFAVFLNGPAADVSTRYTRRAQTFDEAARLGRLAASGAGQVAAVARLS
                     RTSGVCLACEHVTLHRKEPVGEGHALAAIAAAEAEAERLARFGADEIAIKESRDRAAA
                     WRIVLKRAQNQYRLQAASGATQGEPRGAVVDAEISLARMGDLTLLFCPGELAHETGED
                     LAEVVVEGQKAAQAGATVTTAAPTETVTTTATTTPVVPSVGTAPDAPNARAARVARDD
                     VWVVGYSNGHLGYLAPEQAAPDAYERVMSSITPESIHEIMQAAMRLSSKGMCMDLC"
     gene            complement(2434..3375)
                     /locus_tag="PLM74_08690"
     CDS             complement(2434..3375)
                     /locus_tag="PLM74_08690"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HON43189.1"
                     /translation="MRMPDNQVTEYLAEVNRMAQVEEGNGLVEVGLPVSSCSARLRRM
                     FDSLRPAEKKVASYVLDNGGQVIYQSITELANATGTSDATIIRLANTLGYSGYQELKI
                     ALARELVSPDKNIHDDIEPGDTLPVAVKKAFRANVQAVSDTLDVLDLDSLKDAVDAVI
                     AAKQVYLYGVGTSALAAQDAYYKLLRVGIHANFYADMHMQAMSTALISADDVVIGFSH
                     SGSTKDVVDIIDLAKQRGARVICITNRIRSPLARLADIMLRTASEETPFGSGGMPSMM
                     AQLSVVDALFVGVSLAIYDQAIEFIERTGETVKDRKY"
     gene            complement(3690..4085)
                     /locus_tag="PLM74_08695"
     CDS             complement(3690..4085)
                     /locus_tag="PLM74_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43190.1"
                     /translation="MRRRRRAWRHGSCILSRQSIAIQEDVSARIVEGTSLDDVDPLEI
                     LRLRNTIERYRGDAVLLELSDLDFAKALGLVDANGNTRCLTPTGLLLAGKEQSIQRRL
                     PTHEAAFQVLSDVDVEVNQFYRSPLSGRP"
     gene            4469..>4758
                     /locus_tag="PLM74_08700"
     CDS             4469..>4758
                     /locus_tag="PLM74_08700"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HON43191.1"
                     /translation="MASSMLTLRPEASTVVSIDCQPKILSIVADSDTLAPRAASLVRV
                     ARLLGVPVVRVEQNPSRLGPTVPELAEAMGDAEPVAKMTFSLFRTPEAEKAV"
BASE COUNT          957 a         1496 c         1338 g          967 t
ORIGIN      
        1 cgtctccgcc tgcctgccag aagttgatcg tggtctgttt cgcggcgctc gcaggcacaa
       61 ttgccgcagc gagcatgaaa cacgcgatga taaccatggc aacacgcttc atctgtcaaa
      121 cctcctgatc attattggag tcagcacgat tcggcctacc ttctgcacaa aacacccttc
      181 cattgctggt cgaactcatc cccgccatcg atattggcgc tcacgaatac cggcggttca
      241 actcccatgg cgcaaagccg ctctaccacc tccgcctcaa tcatgttgat aatagctgcc
      301 cccgcaactg tcgaagttgg gcatatctgc accgcggagc cgtctgcctc cagcactgcg
      361 tcgccgggca cgccgcagtt gtcgatcacc acgtcagcca cgtcaaacag gtgcttcccg
      421 ctggagtggc gcgacggcac gcttcgcgaa tgcgcaaggg atgtgaggac tattactgga
      481 atcctccgag ccttagcgat cagggccatc tccaccggaa cggcattccg ccccgaattc
      541 gatatgatca taagggcatc gccggggccg attcccgaat ggtccaggat gatctgcgcg
      601 tacccttcca gtcgctccat tgctgtggac ttaaaggggc cttcatgcag catgagagcg
      661 ggctcaagta tggcgttcac cggcacgagg ccgccggccc ggtagaaagc ttcttcggcg
      721 atgatgtgtg aatgcccggt gccaaacaga tacagtctgc cgccgccgcg tatggctgca
      781 gtcacgatgt cggcagcccg acctatcgcc tctagctggg tcttttcaat ctcgtcaaga
      841 atccgccgta cgcggttgag gtagttctga gctaacataa gtccatgcac atccctttgc
      901 tcgaaagtct catggctgcc tgcatgatct cgtgtatcga ttccggtgta atgctcgaca
      961 tcacccgctc gtaagcgtcc ggcgcagcct gctcaggggc cagatatccg agatgaccgt
     1021 tggaataccc aactacccag acgtcatctc gggcgactcg agcggcccga gcgttcgggg
     1081 cgtccggggc ggtccccacg ctcgggacga ccggggtagt agtcgcagtc gtcgtgacag
     1141 tctccgtagg cgccgcagtc gtgacagtag ccccggcctg ggcagctttc tgcccttcca
     1201 ccacgacttc agccaggtcc tcgccggttt cgtgagcaag ctctcccggg cagaaaagca
     1261 gcgtcagatc gcccatgcgc gcaaggctta tctcagcatc gaccaccgcg ccgcgtggct
     1321 cgccctgcgt cgcgcccgat gcggcctgca gtctgtactg gttctgtgct cgcttcaaga
     1381 ctatcctcca tgcagcggca cgatcgcgag actccttaat tgcgatctca tccgccccga
     1441 acctagcgag gcgctcagcc tcagcttcgg ctgctgctat ggcggccaga gcgtgccctt
     1501 cgccgaccgg ttccttgcgg tggagggtaa cgtgttcgca tgcaaggcac acgccggatg
     1561 tgcgcgacag ccgggcaact gcggcaacct gcccagcgcc cgacgctgcc agccttccta
     1621 gtcgcgccgc ctcatcaaag gtctgagccc gccgcgtgta ccgtgtgctc acatccgccg
     1681 ccgggccatt cagaaagaca gcaaatgccc gctccgcccc tgacgtgcgc atctcgtgtt
     1741 cgcacgcgtc caccatgaac cctggccagt cccgtgagac tagcgggtta tccgggccga
     1801 gcacagtggg gtgacacgca agactaacca agcgcatggt agcccggggc gcccccgagg
     1861 agccctgatc gagagagcga aactccgcca cagcgatgcg ctgccccatc gggccggttg
     1921 ctccatgccc ggcaagatcg ctccgccgac tggccatgcc gtgtacttgc cctgagccca
     1981 ggtacagccg gcacggaagg agctcggccc acgcctgcgc catcgcttca gccgctgcgc
     2041 tcaatatcgc gtctatggtc ggaggatcga aggaacccga gtccgggaat atgcccgaag
     2101 ggcctgaatg agtgtgggaa cagcagacca ttactgatgc ggagcctgac gccgcgcccg
     2161 cggggacagc atcagtcgcc gactggcgga tcatgcgcgt gaggcggaca tcggagtaga
     2221 gaatgtcaag cgataggatg gcgatgcagc gtccgtcgaa ttcgacggcc agagccctgg
     2281 cgtacagcgg gtcgtgcacg ccgcgcgata cctccttccg ggcttggtaa cccgcaaggg
     2341 ggatcgacgg tggaagctgt attcggccaa gacccgagcc cacgttgaac atgtagcgct
     2401 caccgccctt gcctgccgcc tgaagctcgt ccgtcagtac tttctgtcct tgacagtctc
     2461 gcccgtacgc tcaatgaact cgatagcctg gtcgtatatg gccaaagaga cccccacgaa
     2521 cagcgcatcc accaccgaca gctgagccat catggacggc attccacccg acccgaacgg
     2581 cgtctcttcg gaggcggtgc gcaacataat gtcggccagc ctagccaggg gagatcgaat
     2641 gcggttggtg atgcatatga cacgagcgcc gcgctgcttg gccaggtcga tgatgtccac
     2701 cacgtccttg gtgctcccag aatgggaaaa cccgatgaca acgtcgtcgg cgctgatcag
     2761 cgcggtggac atcgcctgca tatgcatatc ggcatagaag ttggcgtgga ttcctaccct
     2821 gagcagcttg tagtatgcgt cctgggccgc gagagcagat gttcccacac cgtagaggta
     2881 gacttgcttg gcggctatca ccgcatcgac cgcgtccttc aacgagtcga ggtctagtac
     2941 atccagcgtg tcgcttacag cctgaacgtt ggcgcgaaac gccttcttaa ccgccaccgg
     3001 cagagtgtcg ccgggttcaa tgtcatcatg gatgttctta tctggcgaca ccagctctct
     3061 agccaaagct atcttcaatt cctgataccc gctgtacccc aaagtgttgg caagacggat
     3121 tatcgttgca tccgaagtgc cagtggcatt ggccagttcc gtgatggact ggtatatcac
     3181 ctgcccgccg ttatcgagaa cataggacgc gaccttcttc tccgcgggcc tgagcgaatc
     3241 gaacatcctc ctcaagcgcg cagagcagct cgaaacgggc aacccaacct cgacaagtcc
     3301 attgccctcc tcgacctggg ccatccggtt cacctccgcc aagtactcag taacctggtt
     3361 gtcgggcatt ctcatgaagg attactccac cgatttggtg cttccagcgg agctattcta
     3421 caccgcttgg agttttcctt cttgcgttgc ccaaagtttt gtggagatta tctccacgta
     3481 tggcaaaaga aacacgcgcg tgttgccaaa tcgcgcgcgc gtgtttctcg cgcattgttc
     3541 acgtgttttc gcgcgtttac gcctcagtct cgaacctgcc gttctcttcc cgaaagcagt
     3601 ctgccagctc gtagtatgct cctcttccca tacctcgcaa caccagatgt ccttctttca
     3661 ccagaccctt aaggatgcga gtagcctgat cagggccgac ctgacagtgg agaacggtag
     3721 aactggttca cctccacgtc tacatccgat agaacctgaa atgcggcttc atgcgtggga
     3781 aggcgccttt gaatggactg ctccttgcca gccagtagta ggccggtagg ggtcagacac
     3841 cgtgtattgc cattggcatc aaccaagcct agagctttcg cgaaatcgag atcggaaagc
     3901 tccagcagca ccgcatctcc cctgtatcgc tcaatcgtat tccgcaggcg cagaatctcc
     3961 agtggatcca catcatccaa agaagtccct tcgacgatcc gagcgcttac atcctcctga
     4021 atggcaatgg actggcgcga aagaatgcat gagccgtgcc tccatgctct cctcctcctc
     4081 cgcacgcgat atctgcctcg cctcagtact ggttattctc ctcactcgac gcgagacatc
     4141 tgcatcttgc tgctgtggga atgaacgctt cgcgctcaat ggcaggtagc ggatatgcgc
     4201 tgtacgctgc ttgttcgccc cgaactgcaa aatggggcgc cgttgccgac gtgaattcag
     4261 aatgggtctg gttagctata ctgtcactta ccagaagcca agtgtgtagg cgaacgggcc
     4321 aatcaggccc aggatggcat gagcgcccga acgtgtataa taggcttggg gcatgcgcga
     4381 ggacataagt gcaaggacat atgcgcggaa gatacaggtg cgcggaaggc acattgtgcg
     4441 aaacacgagc acagggaggc gaatggtgat ggcgagcagc atgctcacgc ttcgtcctga
     4501 ggcgtcgact gtggtgtcga tcgactgtca gcccaagata ctctcgattg tggcggattc
     4561 ggatactctg gcgccccgtg cagcgagtct cgtgcgggta gcgcgtcttc tgggcgtgcc
     4621 cgtggtcagg gtagaacaga acccgtctcg cctcgggccc actgtgcccg agcttgcgga
     4681 ggcgatgggc gatgcagaac ccgtcgccaa gatgactttc agcctctttc gcacacccga
     4741 ggccgaaaag gccgtaaa
//
LOCUS       DAOTAC010000201         4713 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_15005_length_4713_cov_4.059897, whole genome shotgun sequence.
ACCESSION   DAOTAC010000201 DAOTAC010000000
VERSION     DAOTAC010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4713
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15005_length_4713_cov_4.059897"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="PLM74_08705"
     CDS             <1..399
                     /locus_tag="PLM74_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43192.1"
                     /translation="GRALVDGGILNPIPANYLAALGVDVSIAVDVMPVLRPSPEQLGK
                     APSIVSTLINSFDIMGRLVAAPVACMATVVIRPQVGEVFGAEFWRAPELITEGFRAAE
                     AAVPAIGEAAGLEPVAVANMPPPDYAMVIA"
     gene            424..1323
                     /locus_tag="PLM74_08710"
     CDS             424..1323
                     /locus_tag="PLM74_08710"
                     /inference="COORDINATES: protein motif:HMM:NF022604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HON43193.1"
                     /translation="MIPQPALPALSPSLAMGVVEEMRFWLQIMSDHARIIRSGLDVTD
                     ESLFRCVDCLGVGIDELLTAVQSACPPFPQGQVECLIAEANRRVTPLRELKRNLAAMV
                     RECRINIQLPADMLEHIRREADFFLGIINRPVGWPVPTRSDLEIPAPPAEANLPACLA
                     PRSVIPYMGQGGAVVLMDEILFFADINAEHAAVLSLFFRPIVQDELSEKARELGEMGM
                     GPFREMSQARLAGASLADMCGMIARSRDAVNAWVGYLRCLKESIGRCSVPTGQVNFAY
                     HLLHHMEREACYTLELMDMVQGV"
     gene            complement(1404..2759)
                     /locus_tag="PLM74_08715"
     CDS             complement(1404..2759)
                     /locus_tag="PLM74_08715"
                     /inference="COORDINATES: protein motif:HMM:NF028468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HON43194.1"
                     /translation="MYRQTALGRETISGPGPGPGPKPGQPLALGLAPTLALAAAGLAA
                     SFWVARVNYLFFHAAVENFSVIVAALTCVLALASSRYARSDLLLFIGHAHALVGGIDF
                     LHTMAYKGMCILPLPTPDTATQLWIAGRYVWAAMFLMAPALASRRLPRRPATAALGVT
                     AVALAWLILRGGIFPACFVEGLGLTPFKIVSEYLVIAAVIAGSLLLAGKRNTIDDSLF
                     NALMGAAGATVVSEFCFTLYHDVYATPNFLGHIFKVIAYSFLFRGVVLRGLQAPYDSI
                     FAELQRSAIRDSLTGLYNRFGFIQIAQREMDETADKPGARTAALMADLDKFKHVNDTF
                     GHLAGDLVLIQFARILTSSVGPADTVARLGGDEFVILLSGADGERATAVASIIRQQMR
                     AWTQQSETAAKLDVSIGVALWTPDIGGDLDTLLREADDAMYKEKESKRAQTAVAGPGP
                     E"
     gene            3198..3770
                     /gene="lepB"
                     /locus_tag="PLM74_08720"
     CDS             3198..3770
                     /gene="lepB"
                     /locus_tag="PLM74_08720"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HON43195.1"
                     /translation="MEYVQSIALAILLAMVIMTFVGRSFVVEGASMEPTLHNRERIIV
                     EKVSYRFRVPARGEIIVLRNPWRPDTEGWARAVEAMRELVDFSGSMRPYIKRIIGVAG
                     DTIEISDGQVYLNGKELHEPYVAASPWSDYGPGTVPEGHVFVMGDNRNNSDDSRGSVG
                     FLRTDRIVGRAVVRYYPLNKATVMKRPDVY"
     gene            complement(3910..4374)
                     /gene="mscL"
                     /locus_tag="PLM74_08725"
     CDS             complement(3910..4374)
                     /gene="mscL"
                     /locus_tag="PLM74_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017250903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HON43196.1"
                     /translation="MLKEFRDFAMKGNVMDMAVGIIIGGAFGKIVSSLVNDVIMPPIG
                     MLLGKVDFSNLFINLSGEHYASLADAKAAGAATVNYGLFLNTVIDFIIVAFAIFLMVK
                     WINKMRATAEALEKRPPVVPAAPTTKECPYCLSTIPIKAIRCAHCTANLSDK"
     gene            complement(4477..4560)
                     /locus_tag="PLM74_08730"
     tRNA            complement(4477..4560)
                     /locus_tag="PLM74_08730"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4525..4527),aa:Leu,seq:tag)
     gene            complement(4594..4670)
                     /locus_tag="PLM74_08735"
     tRNA            complement(4594..4670)
                     /locus_tag="PLM74_08735"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4634..4636),aa:Pro,seq:cgg)
BASE COUNT         1000 a         1306 c         1433 g          974 t
ORIGIN      
        1 ggtcgcgcgc tcgtggatgg aggcatccta aacccaatac ccgcgaacta cctggccgcc
       61 ctgggagtcg atgtttcgat cgctgttgac gtaatgccgg tgctcaggcc atccccggag
      121 caactgggga aggctccctc catagtctct acactaatca actcgttcga tatcatgggt
      181 cgcctggtgg cggcgcccgt cgcctgcatg gcgacggttg tgatcaggcc tcaggtgggc
      241 gaggtattcg gcgcagagtt ctggcgtgcg ccggagctga tcaccgaggg gtttcgggcg
      301 gctgaggctg ccgtgcctgc catcggggaa gcggcaggac ttgagcctgt cgctgtcgcg
      361 aacatgcctc cgcctgacta tgctatggtg attgcctaaa gcggaggtga cttcgtcgtg
      421 agcatgatcc cgcaaccggc tctgccggcg ctctcgccca gtctggcgat gggcgtagtc
      481 gaggaaatgc gattctggct tcaaatcatg tcagaccatg ccaggataat ccgaagcgga
      541 ctggacgtaa ccgatgaaag cttgtttcgc tgtgtggatt gcctgggcgt gggcattgat
      601 gaactactga cggcggtgca gtcggcatgc ccgcctttcc cccaggggca ggtggaatgc
      661 ctgatagcag aagcgaatcg gagagtgact cccttaaggg agctcaagcg caacctggcg
      721 gccatggtaa gggagtgtcg catcaacatc cagctgccag ccgacatgtt ggagcacatc
      781 cggcgtgaag ccgacttctt cctgggtatc ataaacaggc ctgtcggatg gcccgttccc
      841 accagatcgg atctggagat cccggcgcct cctgccgagg caaacctccc ggcatgcctt
      901 gcgccgagga gtgtgatccc ctacatgggc cagggtggcg cagtggtcct gatggatgaa
      961 atcctgttct ttgccgacat caatgccgag catgccgcag tgttgagtct gttcttccgc
     1021 ccgatcgtcc aggatgagct ctcagagaag gcccgggagt taggggagat gggcatgggg
     1081 ccattccgcg agatgtctca ggcaaggctg gcgggagcat cgcttgcaga catgtgtggg
     1141 atgattgccc gcagccgcga tgcggtgaac gcgtgggtgg gctacctgag atgcctgaag
     1201 gaatcgattg ggcgctgcag tgtacctact gggcaggtga acttcgcgta tcatctgctt
     1261 catcacatgg agcgagaagc ctgctacacg ctggaactga tggatatggt gcaaggtgtg
     1321 taggcatcgg ggcaagcttt ggcaagccgt ggcttgaagc acccatagca tgcatggcca
     1381 ccaggatagc gggaccggga tccctactcg ggtcccgggc ccgcgactgc ggtctgggct
     1441 cgctttgact ccttctcctt atacattgca tcatccgcct ctcgaagcag agtatccagg
     1501 tcgcctccta tgtcgggtgt ccacagggcc acgccgatgc tcacatcaag ctttgctgcg
     1561 gtctcggact gctgggtcca tgcgcgcatc tgctgcctta ttatgctggc gacagctgtt
     1621 gcccgttctc catcggcacc cgaaagcaag ataacgaatt cgtctccccc aaggcgcgca
     1681 accgtgtcgg caggcccgac cgaggatgta aggatacgtg caaactggat gaggaccaag
     1741 tcgccggcga gatggccgaa tgtgtcgttt acatgcttga acttgtcgag atccgccatg
     1801 agggcggccg tccgagcacc gggcttgtcg gcggtttcgt ccatttcgcg ctgcgctatc
     1861 tggataaaac cgaatcggtt gtacagccct gtgagagaat cgcggatagc gcttctctga
     1921 agctcagcga agatcgagtc gtatggagcc tgtagcccgc gaagaaccac gccccgaaac
     1981 aagaagctat aggctattac cttgaagatg tgcccaagga agttgggagt agcgtaaacg
     2041 tcatggtaga gggtgaaaca gaactccgag acaacggttg ctcctgctgc gcccattagc
     2101 gcgttgaaga gcgagtcatc gatcgtgtta cgcttccctg caaggagcag cgatccggcg
     2161 ataacggcgg ctatcactag gtactcgctt acgatcttga acggggtcag ccctaaacct
     2221 tcgacaaagc acgcggggaa gatgcctccg cgtaggatta accatgcaag ggctaccgcg
     2281 gtcacgccaa gtgcagccgt cgccggcctt cgcggaagcc tccggcttgc cagcgccggt
     2341 gccattagga acatcgcagc ccacacatac ctgccggcaa tccatagttg tgtagcagtg
     2401 tccggtgtcg gcaatggcag gatgcacata cccttatagg ccatagtgtg taggaagtct
     2461 atgcccccga ccagcgcatg tgcatgcccg atgaagagca gcaggtcgct gcgggcatac
     2521 cgagaagacg ccagcgcaag cacgcaggta agtgcggcca cgatcaccga gaagttctca
     2581 accgcagcat ggaaaaatag atagtttact cgggccaccc agaacgatgc ggctagacct
     2641 gctgccgcca gcgccagcgt cggcgccagc cccagcgcta gtggttgccc tggcttgggt
     2701 cccggtcctg gtcccggtcc cgatatcgtt tcccgcccca gtgccgtttg ccggtacatt
     2761 cccatgcccc ccctcttgct ggattgtcca gcgtatcatc gtactcgtcc ggagagtgag
     2821 acgtaccatt cgcaaaagaa cgggtgcctt gagcttgcca cagagcgcgt aagggggtat
     2881 tatcgagtcg ggaccgtgca cggcgcggga acgactagga catcattatc gttaatgaca
     2941 gaataggcat atgtccaggt tgggataagg tggtcgcgta tcaaatgact gagaatccga
     3001 aaattgagga cgcggtcgaa cagaagatca gcgacacgac tgacgacacg ctcgacatga
     3061 ctgaacaagc ggacgagcag gacgtggaaa cgctgggtga atcctctgac atcacgtcgg
     3121 acttcgattg gggtgcagcg caggccatca ggctcgctga ggccaagcgc cgaagaaagg
     3181 ctgcggttcg tgaggccatg gagtacgtgc agtccatcgc attggcgata ttgctggcaa
     3241 tggtgatcat gaccttcgta ggcaggtcgt tcgtagtgga aggcgcatca atggagccca
     3301 ccttgcacaa ccgcgaacgg attatcgtgg agaaggtgag ctacaggttc cgcgttcccg
     3361 ccagaggcga gatcatcgtt ttgcgcaacc catggcggcc tgacacagag ggatgggccc
     3421 gtgctgtgga agccatgcgt gaactagttg acttcagcgg ttcgatgcgt ccgtacatca
     3481 aacggatcat aggtgtagca ggcgatacca ttgaaatatc ggacggccag gtctacctca
     3541 acggtaagga attgcatgag ccatatgtgg ccgcgtctcc ctggagcgac tacggcccgg
     3601 gcacagtgcc ggaaggccat gtgttcgtga tgggcgataa ccggaataac tcggatgaca
     3661 gccgaggaag cgtaggcttc ctgaggaccg atcggattgt gggaagagca gtagtgcggt
     3721 actatcctct aaacaaggca actgtgatga aacgtccaga cgtgtattga acgaacctat
     3781 agacgagatt gtggctgtgg caatcacgcc ccgggtcttt gcgcaaagac ccggggcgta
     3841 gcgtttcaca tgccgattac ttggacgtcc atcgtgtcgt cgacgcctcg tcgacgcctc
     3901 atcgatgctc tacttgtcag acagatttgc ggtgcaatgc gcgcatctga ttgccttgat
     3961 cggaatagtg gacaggcagt acgggcattc cttcgtggtg ggagctgcgg ggactaccgg
     4021 tggcctcttc tccagagcct ctgcggtcgc tctcatcttg ttgatccact tgaccatgag
     4081 gaatatcgcg aacgcaacga tgatgaagtc aatcactgtg ttaaggaata gcccgtagtt
     4141 gaccgttgct gctccggccg cctttgcgtc ggcgagagac gcgtagtgtt cgcccgacag
     4201 attgatgaac aagttcgaaa agtcaacctt gccaagcagc attccaatcg gcggcatgat
     4261 gacatcattc accagtgagc tcacgatctt tccgaacgct ccgccgatga tgatgcccac
     4321 ggccatatcc atgacgttac ccttcatcgc aaaatcccgg aattctttca gcatgtaggt
     4381 cattcctcct gccgtctcgt cgcttctaag gtttggcctc gggtcgcagg tacaaaaata
     4441 gctgccctgg gcagcttgga gtagattgtg agttgctggt gcgagagggg ggagttgaac
     4501 ccccacgggt tgccccgtca gatcctaagt ctgatgcgtc tgccattccg ccactctcgc
     4561 acgcatggat gcgcctgcat gacggaacaa gaatggtcga ggtgccggga cttgaacccg
     4621 gggcctcttg gtcccgaacc aagcgcgcta ccaaactgcg ctacacctcg acctcgcgcc
     4681 catatgaggc acacccatag tatagagcat ggg
//
LOCUS       DAOTAC010000202         4707 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_15031_length_4707_cov_4.696690, whole genome shotgun sequence.
ACCESSION   DAOTAC010000202 DAOTAC010000000
VERSION     DAOTAC010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4707
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15031_length_4707_cov_4.696690"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..907
                     /locus_tag="PLM74_08740"
     CDS             <1..907
                     /locus_tag="PLM74_08740"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HON43197.1"
                     /translation="RLTNSSLKQNEFNPALHAATISRVVERFAPDAVFFMMDLSLEAG
                     ALGLPVRYPLFESPTVEEHPVKQVSDLDKLRVVDPLEDARICAYIRVMELMSRFISIP
                     RAAYVTGPFTLAGLLMGASDIAMATITDPSLVHQVVQFCKDICTRYARALTEAGADII
                     VILEPTATFLSPQAFAEFCGAYVREVIASPGVAGNAVLHICGDTSHILREMCATGACA
                     LSLDSPVDFPTAASRVDPEVILIGNIDPVGVMVQQSPHDVACATRELLDSMTRYDNFV
                     LSTGCDLPYETPHANIDAFMNEGRR"
     gene            904..2691
                     /locus_tag="PLM74_08745"
     CDS             904..2691
                     /locus_tag="PLM74_08745"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HON43198.1"
                     /translation="MTEHFRLTMPARGIDANTPSVRVCAGETLLDAIRRAGGSLYAPC
                     AGRGICGKCRIIARGGLAAPTDAERDHLTPAQLAAGVRLACQARIIGDVEIHAVGAEP
                     SDAWPDIAGSASILLQTQGSGPARFSRSEHEPQPQSEPESCPQPQPQRQPESLPSSQL
                     EAHPAGLGVALDIGTTTLVAYLVDRNTGLIRDAAGALNPQAVHGADLISRIAFSETPG
                     GPETLRREVTQAADELVRTLLQRSDVSGDDVRCVSAVGNTCMHHLFLGIPASGLGRIP
                     YAAAVLDTGIMSAASAGLASVNPEAILFFLPNIAGFVGSDALAVALVAGMDECSRPTL
                     AIDIGTNGEILLAANGRILACSAAAGPAFEGVNISSGMIACQGAIDWVGFAGLPPHTD
                     IEIHVIGNVEPRGICGSGLISLVAALRQAGLVSEAGAFEPEAIDPESPLARRIATGPG
                     PGGIEFILSDPGDAVPRDDAVPARVTLTQRDIRELQLAKAAIRAGAEVLLDEAGISAS
                     DIEQIVLAGAFGTYLDKHAAHAIGLLPGAPQARITALGNAAGEGAALAVANCDAYREA
                     RRLAGIIEHIELGANPRFMQVFADSMLLA"
     gene            2722..3132
                     /locus_tag="PLM74_08750"
     CDS             2722..3132
                     /locus_tag="PLM74_08750"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HON43199.1"
                     /translation="MKREMRKETRKVIEQPRAIDLPAESVPSVQILEALGVNTAPADV
                     IRSTLESVNAANVMIARVSPGCQAVPIEAQKVLFSLLDAQSIGVTLTDSLLMIPMKSI
                     SLILPLAEALPERLRVFSMCHTCRRSETCMRIIL"
     gene            complement(3929..4675)
                     /locus_tag="PLM74_08755"
     CDS             complement(3929..4675)
                     /locus_tag="PLM74_08755"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HON43200.1"
                     /translation="MRICDCHAHVGQFDDHMSGHRASYSPQALLSEMDAAGVDLAVVS
                     NISAIDDPGGANDEMAAWARRYPRLIPLMWVTPEISRPDKVLEYLGIGFRGVKLHPTA
                     GKYRADCAAVRPLLDVCREAHIPALYHCAADQFSAPALFGQVAADYPEVPIILAHMNM
                     FGRAQDAIAVAESHSNVYLDTSWVRPERVLEAVRRVGADKVLWGTDAPLGGTGHYRRD
                     RAREYLRKHAGTLEYEAMTWSNAACLFCLE"
BASE COUNT          932 a         1509 c         1369 g          897 t
ORIGIN      
        1 gcgcctcacc aactcgtctc ttaagcagaa cgagttcaac cccgcgctgc atgcagccac
       61 catatcgcgt gtagtcgaaa ggttcgcacc ggacgcggtc ttcttcatga tggacctgtc
      121 gcttgaggcc ggagcgctgg gccttccggt caggtatccc ctgttcgagt cgcccacggt
      181 agaggagcat cctgtgaaac aagtgagcga cctcgataag ctccgcgtgg tggacccttt
      241 ggaagacgcc cgcatctgcg cgtacataag ggtcatggag ctgatgagtc ggttcatatc
      301 gattccccgt gcagcttacg tcacaggccc cttcacgctc gcaggactgc tcatgggcgc
      361 aagcgatatc gccatggcca ccataaccga tccgagcctg gtgcaccagg tggtccagtt
      421 ctgcaaagac atctgtaccc ggtatgcccg cgccctcacc gaagccgggg ccgacatcat
      481 cgtgatcctc gagcccaccg ccaccttcct ttcgcctcag gccttcgccg aattctgcgg
      541 agcgtacgtc cgagaggtga tcgcgagccc gggggtggcc ggcaatgcgg tgctgcacat
      601 ctgcggcgat acgtcacaca tcctgcgcga aatgtgcgcc accggcgcct gcgctctgag
      661 cctggattcg ccggtggatt tcccgaccgc ggcaagccgt gtcgaccctg aagtgatact
      721 tatcggcaac atcgaccctg taggcgtcat ggtgcagcaa tcgccgcacg atgtcgcctg
      781 cgccacacgg gaactgctcg actcaatgac gcggtatgac aacttcgtac ttagcaccgg
      841 gtgtgaccta ccgtacgaga cgccccacgc aaatatcgat gctttcatga atgagggaag
      901 acggtgaccg agcactttag actgacaatg cccgcgcgcg gcatagacgc gaacacaccc
      961 tcagtacgtg tttgtgcggg cgagaccttg ctggacgcca taaggcgcgc cggcggaagc
     1021 ctctatgcac cttgtgccgg gcgaggcata tgcggcaagt gccgcatcat cgcaagaggc
     1081 ggcctcgcag ctccgacaga cgccgagcgt gatcacctca cccccgcgca gcttgcagcg
     1141 ggcgtaaggc tggcttgcca ggcccgtatt atcggcgatg tcgaaataca cgccgtcggc
     1201 gccgagccca gtgacgcctg gcccgatatc gccggttcag ccagcatact cctgcaaacg
     1261 caaggctcgg gcccggcccg cttcagtcgg tcagagcacg aaccacagcc ccagtcagag
     1321 cccgagtcct gccctcagcc gcagccccag cgccagcccg agtccctgcc ttcgtctcag
     1381 ctcgaagccc acccagcggg acttggagta gctctggata tcggcaccac cacacttgtg
     1441 gcgtatctgg tagatcgcaa tacgggactg ataagagacg cggcaggcgc actcaacccg
     1501 caggcagttc acggcgcaga tttgatttcg cgcattgcct tttccgaaac cccgggcgga
     1561 cctgagacac ttaggcgtga ggtgacacag gccgccgatg aacttgtgcg tacactcctg
     1621 cagcgctccg acgtatcggg cgatgatgtg agatgtgtga gcgccgtggg aaatacctgc
     1681 atgcaccatc tgttcctggg catccccgcc agcgggcttg gccggatacc ctatgctgct
     1741 gcggttctgg acacaggcat aatgtcggcc gcgagcgccg gcctggcatc agtcaaccct
     1801 gaagcgattc tcttcttcct gcccaacata gccgggttcg taggctccga cgcgttggca
     1861 gttgcgctcg tagccggtat ggatgagtgc tcccgcccca ccctggcgat agacatcggc
     1921 accaacggcg agatactgct cgccgcaaat ggcagaatcc tcgcatgctc cgcagctgca
     1981 gggcccgcat ttgagggcgt caacatatca agcgggatga tagcctgtca gggcgctatc
     2041 gattgggtcg gtttcgccgg gctcccgccc cacaccgaca tcgaaatcca cgtgataggc
     2101 aacgtcgagc cgcgcgggat atgcggatcc gggttgatca gcctggtggc cgcgttgcga
     2161 caggcggggc tcgtatccga ggccggcgca ttcgagcccg aagccattga tccggaaagt
     2221 ccgctcgccc gccgcattgc tactggccct ggcccaggcg gcatcgagtt catcctctcg
     2281 gacccgggtg atgctgtgcc gcgcgacgat gccgtccccg cgcgcgtcac cctcacccag
     2341 cgcgacatac gcgaactgca gctcgcaaag gcggccatca gagccggagc agaggtgctt
     2401 ctcgatgagg ctggcatcag cgccagcgac atcgaacaga tcgtgctggc cggcgccttc
     2461 ggcacgtacc tcgacaagca tgccgcgcac gctataggcc tccttcctgg agcgccgcag
     2521 gcccgcatca ccgcgctcgg aaacgccgca ggggagggtg cggcccttgc tgtcgcgaat
     2581 tgcgacgcat accgcgaagc ccgtcgcctc gctggtatca ttgagcacat tgagctggga
     2641 gccaacccca ggttcatgca ggtcttcgca gactccatgc ttcttgcgtg aatactacga
     2701 acgcttacgg agaacgcgga gatgaagaga gagatgcgaa aggaaacgcg caaggtaatc
     2761 gagcaaccgc gagcaataga cctgcctgct gagtcggtcc cgtccgtcca aatactggag
     2821 gccctcggcg tgaacacagc gcctgcggac gtgatccgct caaccctcga atccgttaac
     2881 gccgctaacg tcatgatagc ccgcgtatcg cccggatgcc aggccgtccc gattgaagcc
     2941 cagaaggtac ttttctcgct gctggacgca cagtccatcg gcgtgaccct gacagactca
     3001 ctgttgatga tccctatgaa gtccatctcc ctcattcttc cgctggccga ggcgcttcct
     3061 gagaggctga gagtgttcag catgtgccac acatgccgcc gcagcgagac gtgcatgcgg
     3121 attatcctgt gatagccggc gactggatcg agcaccacga ctccaacgcc gatcattgcg
     3181 gcccatgcgt cctggctggg gtctcacagc cgagtgtttc gcgtgggcga tcggggctag
     3241 agtcggggtt agcttgagag cagcgggagg gtgatcggaa gccagaagct gagctcttcc
     3301 cgtgttgcca ggaggtaatt acaggttttt ggatggagaa ggcctatccg cggaaatttt
     3361 ccgccgtatc gcggcgaaaa atctcccttc aaaccgattc tcgcggggga gagtcagttc
     3421 agctggtatg atggttattt atgtatggaa ctctccggga tatcttgggt tatatggcat
     3481 atgataccta atattacctg agcgtgcgtg ttggccctat tccgctctgg cagtttcatg
     3541 atgtcagatg ggcttctatg ggaattcgtg gccgctcgcg gagatttttc gcacgcaagc
     3601 tgcagaaaat ctccggaaat cgctccgatg tatacaaaaa cctgtaattg ccagcggccc
     3661 cgcgcgccgt cgccatgatg cgggccatct gcaaccccgc gatctcagca ccacgccacc
     3721 acgcccgtac cgcacatcgc acgccatctg ggtcgagaca gtcgctgcgt gtcggctcct
     3781 gtaccccaca agacagcttc cccggtcctc ttatgggttt acccgggtca ccggaaatct
     3841 acttgactct tgcgagcaca tctgtcccgt aggacatagg ttcgggccgc cgacgccaca
     3901 tgccagaggc caaatctggg gccgagcctc actcaaggca gaacaggcac gccgcgttgg
     3961 accacgtcat ggcctcatac tccagcgtgc cggcatgttt cctgagatac tcccgtgccc
     4021 ggtcacgccg gtagtgccct gtgcctccca gcggcgcatc agtgccccat agaaccttgt
     4081 cggccccaac cctccgcaca gcctccaaaa cccgctcagg ccgcacccag ctggtgtcga
     4141 ggtatacgtt ggagtggctt tcggcgaccg ctatggcgtc ttgggcacgg ccgaacatat
     4201 tcatatgagc cagtatgatg ggcacctcag ggtagtcggc agcgacctgc ccaaacagtg
     4261 ccggcgcgga gaactggtca gcagcgcagt gatacagcgc cggaatatga gcttctcggc
     4321 acacatccag cagcggacgg actgcagcgc aatcagcgcg atactttccg gccgtcgggt
     4381 gtagtttcac gccgcgaaag ccaataccta agtactcaag caccttatcc ggccggctga
     4441 tttcgggcgt cacccacata agtgggatca gccgcggata gcggcgggcc caagctgcca
     4501 tttcgtcgtt ggcgcctccg gggtcgtcta tcgccgatat gttggataca accgccagat
     4561 caacccctgc cgcgtccatc tcagacagca gtgcctgagg ggagtacgac gcccgatgcc
     4621 ccgacatgtg atcatcgaac tgcccaacat gggcgtggca atcacagatc ctcaatgtag
     4681 tcgcctccgc attctatgaa ctgccgc
//
LOCUS       DAOTAC010000203         4702 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_15045_length_4702_cov_4.782871, whole genome shotgun sequence.
ACCESSION   DAOTAC010000203 DAOTAC010000000
VERSION     DAOTAC010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4702
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15045_length_4702_cov_4.782871"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="PLM74_08760"
     CDS             <1..685
                     /locus_tag="PLM74_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43201.1"
                     /translation="DSTGERYLRYAITESGISPRVGFGHSSGVHAPSTDEHEEDAHIT
                     EESGVRVEMMRKRMRKMETALANDLRGPTIYGDTNTNGDVDVTLLVWGSTLPAACEAV
                     ALLAADGIRANVMHYTDVWPVSDAAAPLTLRAMPTGEATAGSGGACGPGGAAGPHGGG
                     GTDGTGLLVAVEQNYSGQMSLIHRMITGRAPDLAVLKYDGRQISPREIADGVREGLRR
                     GRRKGVSDA"
     gene            678..1580
                     /locus_tag="PLM74_08765"
     CDS             678..1580
                     /locus_tag="PLM74_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HON43202.1"
                     /translation="MPDTLFDSKQKPVWCPGCGDYGIMAALKQAFVSLGLTPHQIVIV
                     SGIGCGSKLPDYVHAYGYMSIHGRPLAVATGVKLANPELTVIVVDGDGDAYGIGGNHL
                     IHTARRNMDITHIVQNNQVYALTKGQFSPTTDRGRRTSTSPFGVIEAPIMPLALALAA
                     GATFVARGAAFDPRYTAGLMAEAISHPGYALLDVLQPCVSFNKVNTYEWYRQKVYKVE
                     ERAQGVARGGDRRADGHDSSDFHAAMKLAREWGDRIPLGVIYRRTEESYHSSLLGLAR
                     GPLPARALDDMGADGFERLLDEHV"
     gene            1628..2242
                     /locus_tag="PLM74_08770"
     CDS             1628..2242
                     /locus_tag="PLM74_08770"
                     /inference="COORDINATES: protein motif:HMM:NF021536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2267 domain-containing protein"
                     /protein_id="HON43203.1"
                     /translation="MKSFCAQVMARARFGDEEEAERTAVESVAVLARSLPRALAAEVA
                     ARLPAELGEVMRSAAGGGDSSDLPGNGSDSPGDDTGPDVGMPEDWPAITPDEPFDWGV
                     WGGLPDGEGVHHADPTQEPAEEAFGDPMVDAQDFIGPAINMMETESGHDSALGGRDLV
                     SVPMGDDAIARVAAVLSTVCQCVGPDMAARIREALPEEVAEWLG"
     gene            3145..4299
                     /locus_tag="PLM74_08775"
     CDS             3145..4299
                     /locus_tag="PLM74_08775"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HON43204.1"
                     /translation="MSDDMHDPLRDPGPVIAGDTGCESSGDGVPAGSVQRPTIRDIAR
                     AAGVSVSTVSHVLNEYGDISAETEHRVRQVMEQLNYYPSALARRLVAKRSYVLQLLLF
                     AVEGLHHPFFYEVTCGITAEVEKAGYELVLSVKDTRDRRWRETLKRCYEAKVEGIMLM
                     GTLPSAKVLAEVRASGIPAVMIDIPFEGPRVTYVTSDNIAGARRAAEHLISLGHRNIV
                     YVDGYDLPDEHTPLPTISVARRIGYEEALRAHGIEFDPSLVCIGNYTQEGARSAVRAK
                     LESRPDVTAVFAISDVMAFGAMKAVRDLGRRVPSDVAVMGFDDIEAASYVRPALTTIK
                     QQGELMGRSAVCELLRIITNPKRSPKATVLPAELVVRESCGASRLSPRFA"
     gene            4493..>4702
                     /locus_tag="PLM74_08780"
     CDS             4493..>4702
                     /locus_tag="PLM74_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43205.1"
                     /translation="MKRAIVSSLVAFLMLALIVPTGFAAAKKINFTIWTKESEAEGVL
                     QEIVRLTEEFAKSRPGLSFEIVNYGV"
BASE COUNT          995 a         1277 c         1494 g          936 t
ORIGIN      
        1 agactccact ggcgagaggt acttaagata cgctatcacc gagtcgggga tttcgccgcg
       61 cgtcggcttc gggcattcca gcggtgtgca tgcgccttca acagacgagc atgaggagga
      121 cgcgcatatc acggaagaga gcggcgtcag ggtggagatg atgcgcaaga ggatgcgcaa
      181 gatggagact gcgctggcca acgacttgcg cgggcccacg atatacggcg ataccaacac
      241 gaatggagac gtggacgtaa ccctcctcgt ctgggggtcg acactgccgg cggcatgcga
      301 ggcggtcgcg ctcctggccg cagacggtat ccgtgccaac gtgatgcact acaccgacgt
      361 gtggcccgta tcggacgcgg cggcaccctt gacattgcgt gcgatgccga ctggcgaggc
      421 aaccgccggg tcgggcggcg cctgcggtcc gggcggcgcc gcgggtccgc acggcggcgg
      481 tggtacagac ggcactggct tgcttgtggc tgtggagcag aactatagtg gccagatgtc
      541 cctgattcac aggatgatca cggggcgcgc tccggaccta gctgtgctca agtacgacgg
      601 acgccagatc tcaccccgcg agattgcaga cggagtgcgc gaaggtttgc ggagaggccg
      661 aaggaagggc gtatctgatg cctgataccc tgttcgactc caaacagaaa cctgtatggt
      721 gcccaggctg cggggactat ggaatcatgg cagccttgaa gcaggccttt gtatcattgg
      781 gcctcacgcc tcatcagatt gtgatcgtat ccggaatcgg ttgcggcagc aagctgccgg
      841 actacgtgca cgcctatggc tacatgagca tccatggtcg tcctcttgcg gtagctaccg
      901 gtgtgaaact ggccaacccc gaactcaccg tcattgtggt cgacggtgac ggagacgcct
      961 acggaatcgg cggcaaccac ctgattcaca cggccaggcg gaacatggac atcacgcaca
     1021 tcgtacagaa caaccaggtg tacgcgctca cgaaaggcca gttctccccc acaaccgata
     1081 gaggcaggcg cacgtcgaca tcaccgttcg gggttatcga agcgcccatc atgcctcttg
     1141 cgctggcgct ggctgcgggc gccactttcg tggcgagggg agcagcattt gatccaaggt
     1201 acacagcagg gcttatggcc gaggccataa gccatcccgg ctatgcgctg ctcgatgttc
     1261 tgcaaccctg cgtcagcttc aacaaagtca atacatacga gtggtacagg cagaaggtat
     1321 ataaggtaga ggagcgcgct cagggcgttg ctcggggcgg cgacaggcgc gcggacggcc
     1381 acgactccag cgacttccat gccgcgatga agcttgcacg cgaatggggc gataggattc
     1441 cgctgggggt gatctaccga cgcactgagg aatcgtatca ttcgtcactg ctgggattgg
     1501 ctcgcggtcc tcttccggct agggcacttg acgacatggg tgccgacggg ttcgaacgcc
     1561 tcctggatga acatgtgtga gagtctcagg tgaatgcaag agggcggagg tgcatgcgag
     1621 cgtggacatg aagtcattct gcgctcaggt catggcgaga gcgagattcg gagatgagga
     1681 agaggccgaa cgaaccgccg ttgagagtgt ggcggtgctc gcgagatccc tgcctcgtgc
     1741 gctggccgcg gaagtggcag caaggcttcc ggctgagctc ggggaggtaa tgaggtctgc
     1801 tgcgggcggc ggggacagca gtgatttgcc cggcaacggc agtgattcgc ctggcgacga
     1861 cactggtccc gatgtaggca tgcccgaaga ctggcccgcc atcactccgg atgagccgtt
     1921 cgattgggga gtatggggcg ggctacctga tggtgaaggc gtacaccacg cggatccgac
     1981 acaagagccg gctgaggagg cgtttggaga tccgatggtg gacgcacagg acttcattgg
     2041 gccagctatc aacatgatgg agaccgaatc cggccatgat tcggctcttg gagggcggga
     2101 cttggtttcg gtcccgatgg gagacgacgc catcgcgcgc gtggctgcag tgctcagtac
     2161 agtatgccag tgcgtcggcc ccgacatggc ggctcgtata cgcgaagccc ttcccgagga
     2221 agtggctgaa tggctgggat gagtgactga tgagtgactt gggagtctgg gatgccctag
     2281 aagcccaagg aaccgatatg ccctatggtt caccatcgcg cctcgccgga aatgtcgcca
     2341 agctggccta atgaggatgg cacgacgcga aaacggctgg agctggcata tcgtcaatta
     2401 tcagcagcag ttttggagat accggcaatt tttgagtaca tcgatttcag agggccgccg
     2461 gaccgcctcc gaatcgcgaa aatcgggggg tatccggccg gtccgggcat ggaaccgggc
     2521 aagggaagcg acataatatc ccataaccta tcttttcctg gcaacagcgc gtgagtgatg
     2581 gcatggcctg gcgccgagat cccagataat aggctcgcat cgaccccgag agcgcccttc
     2641 tcagcgctag tccaccgacc aaccgcctcc catgcaaatc gatgtactca aaaactgcgc
     2701 ctatcggcga aactatcagc aaaaactgac ggcatgcctg cctaaatacc cccgggggta
     2761 tatcactgag ctacagcgat cgccttttcg ccttcggagc tgttcccgcg tgcctatcct
     2821 cgggtcccct tgtgccccag ccgctttcga tgtggtccca gtgaggtggt cgcggtgaga
     2881 tggtcccggc gaggccgcat gccagaagcc aaatccgcgg tcgggcgggt tgacaccgtt
     2941 gcgccaatat cctagtatgg aattagcgac tatatcgtta tagaaactcc tgcatttccc
     3001 ctaacatcct caattagcat tgcagcattc gtaacagcac ctgatcattg cagttgatta
     3061 cctaacctag acgcttttgt gcgccagcac taccgccatg atgccaaagg gccatgagca
     3121 aacggtgagg gtgggcgtgt taggttgagc gatgacatgc atgacccatt gagagacccc
     3181 gggcccgtca tcgccggtga cacgggctgt gaatcgtcgg gcgacggtgt gccggcaggc
     3241 tcggttcagc ggccgacgat aagggacatt gccagagcgg caggtgtgtc ggtcagcacg
     3301 gtttcgcacg tgctaaacga atacggcgac atcagtgcgg aaaccgagca ccgtgtccgt
     3361 caggtaatgg agcaactcaa ctactatcca agtgcgcttg ccaggcgact tgtcgcaaag
     3421 cgcagctatg tgctgcaact gctgctcttt gcagtcgaag gactgcacca cccttttttc
     3481 tatgaagtca cgtgcggcat aactgccgaa gtagagaagg ccgggtatga gctggttttg
     3541 agtgtgaaag acactcgaga tcgacggtgg cgcgaaacgc tcaagcggtg ctatgaggcg
     3601 aaggtagagg ggatcatgct catgggaacg ttaccctctg ccaaggttct tgctgaggtt
     3661 cgagcgagcg gcattccggc tgtgatgatc gacattccct tcgaaggacc cagggtgacg
     3721 tacgtgacgt ccgataacat cgccggcgcc cgtcgcgcgg ctgaacatct catctctctg
     3781 ggccatcgca acattgtcta tgtggacggg tacgacctgc ctgatgaaca tacccctctg
     3841 cccaccatat cggtagctag gcgtatcgga tacgaggaag cgctgcgtgc acacggtatc
     3901 gagttcgatc cgagcctggt gtgcatcggc aactacactc aagaaggagc aagatccgcc
     3961 gtccgggcga agctggaatc gcgcccggat gttactgctg tattcgcgat cagcgacgtt
     4021 atggcgtttg gcgcgatgaa ggccgtccgc gatttggggc gcagagtacc cagcgatgtc
     4081 gccgttatgg gcttcgacga tatcgaagcc gcgtcttacg tgcgtccggc actgactact
     4141 attaagcagc aaggcgagct gatgggtaga agcgctgtgt gtgagctgct gcgcataatt
     4201 acgaatccca agagatctcc gaaggcgact gtccttcctg cggaacttgt tgttcgcgag
     4261 tcatgcggcg ctagccggct gtcgccgcga ttcgcgtgag tctccgctag ttggcatgaa
     4321 accgcgtgag attgcacgaa accgaatgac tgcgttcgga ggtgatgctc tggcagacag
     4381 ggtgttccgg atgtctgcga atgcttgagt acgtgggtca aagtccagtg cagtctagtt
     4441 tggccgcatg ggcggccgat catcgggttg attatgaaag gaggctactc tagtgaagag
     4501 ggcaattgtt tcatcattag tggctttcct catgcttgcg ctgatcgtac ctaccggttt
     4561 tgctgcagcg aagaagatca acttcacaat ttggaccaag gagagcgagg ccgaaggggt
     4621 tttgcaggag attgtgagac tcacggagga attcgcaaaa tcccgccccg ggctgtcttt
     4681 cgagatcgtt aactacggcg tc
//
LOCUS       DAOTAC010000204         4690 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_15091_length_4690_cov_5.204962, whole genome shotgun sequence.
ACCESSION   DAOTAC010000204 DAOTAC010000000
VERSION     DAOTAC010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4690
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15091_length_4690_cov_5.204962"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1748
                     /locus_tag="PLM74_08785"
     CDS             <1..1748
                     /locus_tag="PLM74_08785"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HON43206.1"
                     /translation="EAERLLVQLIMLGIPTITFVRARVVAELIYKYAVESLRRQAPSL
                     AGKIKPYRGGYLPSERREIERQLFAGELLGVVSTNALELGIDIGSLDASLIVGYPGTI
                     ASTWQQAGRAGRKGEESLAVFIAHDLPIDQYLMKRPDYFFDRSPENAIINAANPYILA
                     GHLRAATVELPVTAAELESFGEYAPALIAILEERGDVIRTRRGWRWAGRGFPASDVKL
                     RNMAENTYAIVDTSSDDGNRVIGTIDELSAFEQVHPEAVYMHEGETFLVSDLNLTEKV
                     AYIHRADVDYFTQSVTETKVQIDTEEQAKAWRRSQVNFGDVTVTSLTYMFRKIKFYER
                     DSIGFGKVSLPQHELATAAAWLELPESAARLVAGFGRIATEGLIGIGNAASAVIPLFA
                     MCDPMDIGTAVDSANTGVPTLFIYDRHPGGVGFAQKSYNMIEEVMEACLNLIENCTCE
                     DGCPSCVGSPIPPYAQHDPDATPRGRIPDKETALVILHDLLEREPYVPVRPPVWAKWA
                     ESSGLEAGVDAGAGAGMDAAASDGTAAGAASAAAAAAAHDDRAVQVVVKPLPEGVERR
                     IRKIMDRATSQHKAR"
     gene            1870..2613
                     /locus_tag="PLM74_08790"
     CDS             1870..2613
                     /locus_tag="PLM74_08790"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HON43207.1"
                     /translation="MRRLSSVFLLCMALTLVIGTTAAVGAKPILKVGTSADFPPFEFQ
                     DEKTGAYLGFDIDLIRAVGAAAGMDVQIVNTAWDGLIPGLLTGNYDCIMSAMTITDER
                     LKAVAFSDPYFSAGQVIVTRAKDLSIQTVENLEGKKISVQIGTTGDLAASDIKGATVK
                     RFNMAPDAIQEVRNGGVAACIIDLAVAAEIAQEYKDIKFGEPFTMEYYGIAIRKNNAD
                     LIKKINRALASIKASGEYDKIYADWFGQN"
     gene            2708..3391
                     /locus_tag="PLM74_08795"
     CDS             2708..3391
                     /locus_tag="PLM74_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HON43208.1"
                     /translation="MRHDGGIDVALDFSIIPGIIPALLMGTRMTIELAVIAVAIGLVI
                     GSFAGIARVGGNRLARIVTSIYVDFIRGTPLLVQLFLVYFGLPTLIRRPVPPFIAAIT
                     AMGVNSGAYIAEIVRAGIQSVDRGQMEAARSLGLTYSQSMRYIIFPQALRRIIPPLGN
                     EFIAMLKDSSLVSVIALEELLRKGQVVITRTFRPFEVYMVVALIYLAMTLAISRLVAW
                     SERRLRVVD"
     gene            3384..4106
                     /locus_tag="PLM74_08800"
     CDS             3384..4106
                     /locus_tag="PLM74_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010095376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HON43209.1"
                     /translation="MIRTVGLQKSFGNNHVLRGIDVAIKPHEVVVVIGPSGSGKSTFL
                     RCLNLLERPTAGQVFFRGHCITDPHVNVNEVRREMGMVFQRFNLFPHMSVIDNITLGP
                     MKVRGVSPEEAKQTARDLLKKVGISEKEKDYPSELSGGQQQRVAIARALAMKPMAMLF
                     DEATSALDPEMIGEVLDVIRKLAEEGMTMVVVTHEMGFAREVGDRVLFMDEGVIVEEG
                     TPEQVLGKPQNPRTQAFLSKVL"
     gene            4457..>4690
                     /locus_tag="PLM74_08805"
     CDS             4457..>4690
                     /locus_tag="PLM74_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43210.1"
                     /translation="MGSEGPMGPRGAAGTDGVGAPEWSPAEFRRALWIAIAIVAAGLI
                     LGTLIAAWAGGAIPGPVRLFDLVRRFVPPLGGPR"
BASE COUNT          957 a         1244 c         1526 g          963 t
ORIGIN      
        1 gcgaggccga gcgccttctg gtgcaactca tcatgttggg cataccgact atcacctttg
       61 ttcgcgctcg agtcgtggct gagctgatct acaagtatgc ggtggaaagc ctgcgaaggc
      121 aggcgccgtc gctggcgggc aagatcaagc cttatcgcgg cgggtatctg cccagcgaac
      181 ggcgggagat cgagcggcag ctttttgcag gtgaactgct tggggtggta agtaccaacg
      241 ctctggagct gggaatcgac atcggctcac tggatgcgag cttgatcgtt ggctatccgg
      301 gcactattgc ctcaacctgg cagcaggcgg gaagggcagg gcgcaagggt gaagaatcgc
      361 tcgcagtgtt cattgcacac gatcttccta tcgaccagta tctgatgaag cgcccggatt
      421 actttttcga ccggtcgcct gagaacgcca tcatcaatgc ggcgaatccc tatatcctcg
      481 caggccatct gcgcgccgcc acagtggaat tgccggtaac cgcggccgag ctcgagtcgt
      541 tcggggagta tgcacctgcg ctcatcgcca tcttggagga acggggcgat gtgatccgca
      601 ccaggcgggg ctggcgatgg gcagggcggg ggtttcccgc cagcgatgtg aagcttcgga
      661 acatggcgga gaacacatat gcgattgtcg acacatcatc ggacgatgga aatcgggtca
      721 taggcaccat agacgaactc tcggcgttcg aacaggtaca cccggaagct gtgtacatgc
      781 atgagggcga gacttttcta gtgtcagacc tcaacctcac tgagaaagta gcctacatac
      841 acagggcgga tgtggactac ttcactcagt ctgtgacaga gacgaaagtg cagatagaca
      901 cggaagagca ggctaaggca tggcgtcgga gtcaagttaa cttcggcgat gttactgtta
      961 cgagcctcac ctacatgttt cgcaaaatca agttctatga gcgtgacagc ataggcttcg
     1021 gaaaggtgag cctgccccag cacgaactgg ctacggctgc tgcctggctg gagctgccgg
     1081 agagcgccgc acggctcgtg gcagggttcg gacgcatcgc cacagagggg ctcataggca
     1141 tcggtaacgc ggcatccgcg gtgattcccc tgtttgccat gtgcgacccc atggacatcg
     1201 gcactgcggt ggactcagct aacaccggcg ttccaacgct gtttatatac gaccggcatc
     1261 ccgggggcgt gggattcgcc cagaagtcgt acaacatgat cgaggaggtc atggaggcct
     1321 gcctcaacct cattgagaac tgtacgtgcg aggatggctg cccgtcctgc gtgggctcgc
     1381 ccattccgcc gtacgctcag cacgacccgg acgcgactcc gcgcggccgc attcccgaca
     1441 aggagaccgc cctagtcatc ctgcacgatc tgttggagag agaaccgtac gtgccggttc
     1501 gaccgccggt gtgggccaag tgggccgaaa gctccgggct ggaggcgggg gtcgatgcgg
     1561 gggccggcgc cggcatggac gctgccgcgt ccgatggaac tgccgctggt gccgcttctg
     1621 ctgccgccgc tgcagcggct catgacgatc gtgcagttca ggtggtggtc aagccgctgc
     1681 ctgagggcgt ggagcgacgt atcaggaaga taatggatcg tgcgacttcc caacataagg
     1741 ctaggtgaat ttgcctgtag gccctgtata gtgtagaatg tctgtgaaat gtgatcgttg
     1801 acagtggcgc gcgctcagag tatactcatg cagtaacagc gtaacaagat tcgaaaggag
     1861 ctgtgtgtag tgagaagact atcctccgtg ttccttttgt gcatggcact tactcttgtt
     1921 atcgggacga ctgctgccgt tggggcgaaa cctattctga aggttggcac gagcgccgat
     1981 tttccgcctt ttgagttcca ggatgagaag accggggctt acctcgggtt cgacatcgac
     2041 ctgatccgcg cagtgggcgc ggctgcgggg atggacgttc agatcgtgaa cactgcctgg
     2101 gatggcctga ttccgggtct gctcaccggc aactacgatt gcattatgtc ggcgatgacc
     2161 attaccgacg aacgccttaa ggctgtggct ttttcggatc cgtatttctc ggccgggcag
     2221 gtcatagtga ctcgcgctaa ggacctctcg attcagactg tggagaatct tgagggcaag
     2281 aagatttcgg tgcagatcgg caccaccggc gaccttgcag catcggatat caagggcgcg
     2341 acggttaagc gcttcaacat ggcgccagac gcgattcagg aagtcagaaa cggcggcgtc
     2401 gcggcctgca taatcgattt ggcagtcgcg gcggagattg ctcaggagta caaggatatc
     2461 aagttcggcg agcctttcac aatggagtac tacggtattg ccattcgcaa gaacaacgcc
     2521 gatcttatca agaagatcaa ccgcgcactg gcgtctatca aggcttccgg ggagtatgac
     2581 aagatatacg cggactggtt cgggcagaac tgacgcggac cattgaggta tcgatgaggt
     2641 accgaggttg atccaccatg agctggccac ggcgggatag gaactgccgg tggccagtct
     2701 tcatgagatg cgacacgatg gaggaatcga tgtggcgctc gacttttcga taatcccggg
     2761 catcataccc gcactactta tgggcacgcg tatgaccatt gaacttgcgg tcatcgcggt
     2821 agcgatcggt cttgtcatcg gatcgttcgc cggaattgcc cgggtgggcg gcaatcgcct
     2881 ggcgcgcatc gtcaccagca tctacgttga cttcatcagg ggcactccgc tcctggtgca
     2941 gttgttcctt gtatacttcg gcctgcccac tctgatacgt cgccccgtgc ctcccttcat
     3001 cgccgcaatc accgctatgg gcgtaaacag cggagcatat atcgctgaga tcgtccgggc
     3061 gggcatacag tcggtggacc gggggcagat ggaggcggcg cgatcgctgg ggctgaccta
     3121 ctcccagtct atgcggtaca tcatcttccc acaggccttg aggcggatca tacccccgct
     3181 gggaaacgag ttcatcgcga tgctaaagga ctcatcctta gtctcggtta ttgccctgga
     3241 ggagttgctc cgcaaagggc aagtggtgat cacccgcacg ttcaggcctt ttgaggtgta
     3301 catggtagta gcgctgatct atctcgcgat gactctggca atatccaggc ttgtggcctg
     3361 gtctgagagg aggttgaggg tggttgatta ggactgtcgg gcttcagaag tctttcggca
     3421 acaatcatgt gctgcgcggg atcgatgtgg ccatcaagcc gcatgaggtg gtcgtggtga
     3481 tagggccgtc cggctccggg aaaagcacat tcctgcgctg cctcaacctc ttggagaggc
     3541 ccaccgccgg ccaggtgttc tttcgcggtc attgcataac tgatccgcac gtcaacgtaa
     3601 acgaagtccg gcgcgaaatg ggcatggtgt ttcagaggtt caacctgttt cctcacatga
     3661 gtgtgataga caatatcacg ctagggccga tgaaagtgcg cggtgtgtcg ccggaagagg
     3721 cgaagcagac tgccagagat ctgctgaaga aagtgggaat ctcggaaaag gagaaagatt
     3781 acccgtcgga gctatcgggc ggtcagcaac agcgtgttgc catagcccgt gcgcttgcaa
     3841 tgaaacccat ggccatgttg tttgatgaag ccacatccgc gctggacccg gagatgatcg
     3901 gcgaggtgct ggacgtcatc cgaaagcttg cggaagaggg catgaccatg gtggttgtca
     3961 cccatgagat gggttttgcg cgcgaagtgg gtgatagggt ccttttcatg gatgaaggtg
     4021 tgatcgtgga ggaaggcacg cccgagcagg tgctgggcaa gcctcaaaac cccagaacac
     4081 aggcattttt aagcaaggtg ttgtagcgcg gcgaatacgc gaatagtggg cgagccctcc
     4141 gtcgtaggaa tacgaacgga gggatgtctt tatggcccca aaagcggcga gacggaaggc
     4201 gaggattcga caaccgcagc gacaaccgca gcagcgcccg ctgcagaggc ttcaacggca
     4261 gcctgcaaac ggatgtggga ggcaagcagg gcagcagtcg gggcgggctg gccgagcggg
     4321 cgaaacggtc gacaagcgtg tgcgcgctga tggcggcacg tgcagcgcgg gcatgatgcg
     4381 tgcggtggtc tggccgccgg gctgcccctg gccgggagtg tggctgtggc ctgggccttt
     4441 cggtgccggg agtcccatgg gctctgaggg tcccatgggc cctaggggtg ccgcaggaac
     4501 cgacggggtg ggcgccccgg aatggtctcc ggcggagttt cggagggcgc tctggattgc
     4561 gattgccatt gttgcggcgg ggcttatcct cggaaccctc attgcagcct gggcaggcgg
     4621 ggcgattcca ggcccggtca ggctgtttga cctcgtgagg cgatttgtgc ccccccttgg
     4681 agggcctcgg
//
LOCUS       DAOTAC010000205         4689 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_15098_length_4689_cov_4.208459, whole genome shotgun sequence.
ACCESSION   DAOTAC010000205 DAOTAC010000000
VERSION     DAOTAC010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4689
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15098_length_4689_cov_4.208459"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="PLM74_08810"
     CDS             complement(<1..521)
                     /locus_tag="PLM74_08810"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HON43211.1"
                     /translation="MLYEFDKVLDRRNTNCEKWDNLMEVFGREDVVSLWVADMDFASP
                     PEVVEALAERVQHPTYGYTFPSQSLREAAVGWISRRHGWEIDPEWIVITPGVVPGLAI
                     SVLAYTQPGDGVVVQSPVYPPFFRVVESNGRRLLNNELRMGPDGRYAIDWDDLEAKLD
                     DGARLVLLCSPANP"
     gene            720..1070
                     /locus_tag="PLM74_08815"
     CDS             720..1070
                     /locus_tag="PLM74_08815"
                     /inference="COORDINATES: protein motif:HMM:NF024198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43212.1"
                     /translation="MSGFRKHVHKLSGTTATQAGHHHKVSVITGPPWPGAGGHIHRII
                     GATETGKSPHVHQMKGWTGPSMTTGAEHTHSVDISTKETQAHAHTVTGTTAAFAPSRS
                     AALAARTTDGVFGK"
     gene            complement(1176..4256)
                     /locus_tag="PLM74_08820"
     CDS             complement(1176..4256)
                     /locus_tag="PLM74_08820"
                     /inference="COORDINATES: protein motif:HMM:NF024904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HON43213.1"
                     /translation="MRIREVYLDGFGIFHDRRIVIAGGSGVECNSGTASGNQGVVLFT
                     GANEAGKTTLMSFIRGILFGFEGEASRGFYAPERGGRHGGSLTVEMLDGSVDRIVRYA
                     ERGQSRQPAPAVGNMSKALYTNVFAFGLAELALLSSLGSDEVRSRIYSAGAGLGTRSL
                     PEARRRLESKCDSLYKPRGRAPSINAALSEAAELDRQIADLNRDPLEHARLIRDIEQC
                     QESYAQVQASLREAREAQQWYASLDSTRSTWERLCQAAAAQGRLDPEDSSSSGLLAQA
                     GAIRELQLRLDTYLSVAASIPALWAEADAIRADHRRLLGELGVGWNESAVAAFDTSPG
                     VDETVRQMRKRRDALAGAVERAQEVMRAREEAVEAATRQHDRLKQELHRSRKPDYPDR
                     AAIEARIERIGRCQALLMSVAAAGAKVEAARERSGDLAREAAERVEAAAQARGFAQVW
                     LAPVLIGVGSILASLHRPAWAASLLIAVGAVAGIVIARNALRRARAADTVAKASQERA
                     RQAREHEEQLAAAHRRASEELEAAKTALGRVEELEATNAGLGKADELEAVKSGLGSAD
                     EAALAALMERARRDLTDWEGFARIVRDLEEAQMECRRAEKDLHSAHEALGSARAALEA
                     DCDAWRRWLSARGIDSEFSHDGALQAIELIRRAKDARERLGQAERRVEAARTRTREYE
                     EAVNLVLEELGMASVGGAQRSVGAELEGGIERSGGAELEDWAEPGGIRKYARHDTYRA
                     EVDRLVALSAEAQELVDRRKRLAAQVEDLEAQFASVFPASDRTRARADHASHTAAEIQ
                     AALEEAAKRAETLEVKAGELDRGLGSLGRSRAILENRDELARAGAERGAVQGELDALL
                     REWAAHCVCLDLVNSACEKYERERQPQVLQDASAALSRMTGGRWPRIIARVDGLESLD
                     VVGSDGQARPHTRLSCGTEQQLYLAVRCGLVREYCAHAEPMPVMVDDVLVNFDPVRQA
                     AAARVLAELSDTCQVLVFTCHPHTAEHFRQTGLVTAEFALEAVVGPGPVVAGTV"
     gene            complement(4260..>4689)
                     /locus_tag="PLM74_08825"
     CDS             complement(4260..>4689)
                     /locus_tag="PLM74_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43214.1"
                     /translation="AMSSNMRSCAVRVRLCGRGPVHRELAMDGPEALLAAARESSLPG
                     DRFDWIECVQDLTGPDVDRDALKAVGSLISDLLCLTDDMRSDPTRLEAVRGALEQRLG
                     SRADRALSRLVRELSDEDILQIIAKAESLALDLLLEGADS"
BASE COUNT          883 a         1675 c         1276 g          855 t
ORIGIN      
        1 ggattggccg ggctgcacaa aaggaccagc ctggccccat cgtccagctt ggcctcgaga
       61 tcatcccaat cgatagcgta cctcccatcg ggccccattc gcagctcatt gttgagcaat
      121 ctccgaccat tgctctctac gacccggaag aacggagggt acacaggaga ttgcacgacc
      181 acgccatcgc ctggctgagt gtatgccagc actgatatgg caagaccggg cactaccccc
      241 ggagtaatca cgatccactc tgggtcaatc tcccatccat gccggcgcga tatccagccg
      301 actgcagcct cacgcaacga ctgggacggg aaggtgtacc cataggtagg atgctggact
      361 cgctcagcca gtgcctccac gacctcaggg ggcgacgcga aatccatgtc ggccacccac
      421 agagatacga catcctcgcg cccaaagacc tccatgaggt tatcccattt ctcgcagttc
      481 gtgttccttc tgtcaagcac cttgtcgaac tcatatagca tcggccggaa cctcccccaa
      541 agccttgcgt aggcaacacg ctactaatgg attgtagagc agtattcgcc tccgcgtccc
      601 attgtcctgt atgtgctgca cggcacaaca cccctgatac caagcgggta taacatgtag
      661 cgaggtgatc ggacttgctg gaattatggc tcgcgaaatt agaggagagc agttttggcg
      721 tgagtggttt ccgcaagcat gtacacaagc tatcggggac cactgcaaca caggcaggac
      781 accaccataa ggtcagcgtg atcaccggcc ctccgtggcc aggagccgga ggacacatcc
      841 acaggatcat cggcgccact gaaacgggca agagccccca tgtacatcag atgaaaggat
      901 ggaccggacc gtccatgacc acaggcgccg aacacacgca ctccgtggat attagcacca
      961 aggaaaccca ggcgcatgcc catacagtaa ccggcactac cgccgctttc gcaccgagca
     1021 gatcggcagc tctggcagcc cgcaccactg acggtgtatt cggtaagtga cgccgaacgc
     1081 gacccgaacg aattggctga cggtgggttc gctccctcag acgaatccac cgtcggcgct
     1141 tccgccgatt ttggcggtct aggcggtccc gcggtctaga ccgttccggc gactactggg
     1201 cctgggccca caaccgcctc cagcgcgaac tccgcagtca ccagcccggt ctgcctgaaa
     1261 tgctctgccg tgtggggatg acaggtgaag acgagtacct ggcaggtgtc cgacagctcc
     1321 gccagcacgc gcgctgcggc cgcctggcgt accggatcaa agttgacgag cacatcgtcc
     1381 accataacag gcataggctc ggcatgcgcg cagtattcac gcactaaccc gcatctgaca
     1441 gccagataga gttgctgctc agtgccgcag ctcagcctgg tgtgtgggcg agcctggcca
     1501 tccgacccca caacatccaa cgactccagg ccatccacgc gggctatgat gcggggccag
     1561 cgaccgccgg tcatcctgct caaagcagcc gaggcatcct gtagcacctg gggttggcgt
     1621 tcacgctcgt acttttcaca cgcgctgttc acgagatcaa ggcatacaca atgggccgcc
     1681 cattccctga gcaaggcgtc cagctcgccc tgcacagccc ctcgttcagc acccgcgcgc
     1741 gcaagctcat cgcggttttc tagtatcgcc ctggaccgcc cgagcgaccc gaggccccga
     1801 tccagctcgc cggctttgac ctccaacgtt tcggcgcgtt tggccgcttc ctccagcgcg
     1861 gcctggatct ctgctgcagt gtgagacgcg tgatcagccc gcgcccgggt gcggtcagag
     1921 gcaggaaaga ccgaagcaaa ctgagcctca agatcctcca cctgcgcagc caggcgctta
     1981 cgtctatcga ctaattcctg cgcctcggcg ctcagtgcga cgagccggtc cacttccgcc
     2041 cgataggtgt cgtggcgcgc atacttcctg atcccacccg gttcagccca gtcctccagt
     2101 tcggcgcccc cgcttcgttc gatcccgccc tccagctcag cgcctacgct tcgttgggcc
     2161 ccacctacgg acgccatccc gagctcttcc aggaccaggt tcacggcctc ctcgtattct
     2221 cgcgtacgtg ttcgcgccgc ttccacacgt cgctccgcct gaccgagtcg ctcgcgggca
     2281 tccttggccc tgcgtatcaa ctcgatcgcc tgcagcgcgc catcatgact gaactcggaa
     2341 tcaatacctc tggccgacag ccaccggcgc cacgcatcgc aatctgcctc caaagccgcg
     2401 cgggcactgc ccagcgcctc atgggcacta tgtaggtcct tctcagcccg tctgcactcc
     2461 atctgcgctt cttccagatc ccgcactatc cgggcgaatc cctcccagtc ggtcaggtcc
     2521 cgcctggccc gttccatcag cgcagccagc gcagcctcat ccgcgctgcc cagacctgac
     2581 ttcacagctt ccagctcatc tgccttcccc aggcccgcat tcgtggcctc cagctcctcc
     2641 accctgccca gggcagtctt cgcggcctcc agctcctcgc tggcccgccg atgagccgca
     2701 gccagctgtt cctcatgttc ccgcgcctgc cttgcacgct cctgcgatgc cttggctacc
     2761 gtatcggcgg ccctcgcccg gcgcagggcg ttccgggcaa taacgattcc tgcgacagcc
     2821 ccaaccgcga tcaacagcga tgcagcccag gcaggcctgt gtagagacgc gagtatcgac
     2881 ccgacgccta tcaggactgg cgccaaccag acttgcgcga agcctcgcgc ctgggctgca
     2941 gcctcaacgc gctcggcagc ttcgcgggca agatcgccgg accgttccct ggcggcctca
     3001 accttggcgc cagctgcagc tactgacatg agcagggcct ggcacctgcc tatacgctct
     3061 atcctggcct caatcgcagc ccgatcaggg taatccggct tgcggcttct gtgtagctcc
     3121 tgcttgagcc ggtcatgctg gcgcgtggcc gcctccactg cctcttcccg ggcccgcata
     3181 acctcctgcg cgcgctccac cgcaccggcc agcgcgtccc gcctctttcg catttggcgc
     3241 acggtttcgt ccacaccggg agatgtgtcg aacgcagcta cggcgctttc gttccagcca
     3301 acgcccagct ctcccaggag tcgccgatga tcggcgcgta tcgcatccgc ctctgcccaa
     3361 agcgcgggaa tcgacgcggc cacactcaag taggtgtcca gtctgagctg caattctcga
     3421 atcgcgcctg cctgcgcgag cagcccgctg gagcttgagt cctccgggtc gagcctgccc
     3481 tgggctgccg ccgcctggca cagccgctcc catgtcgatc tggtcgaatc caggctggca
     3541 taccattgct gagcctccct cgcctcgcgc aaagatgcct gtacctgagc gtaagactcc
     3601 tggcactgct caatgtcgcg aatcaagcgc gcatgctcca aagggtcgcg gttcagatca
     3661 gcaatctgcc tgtccagctc ggcagcttct gagagggccg catttatcga cggcgcgcgg
     3721 cctctcggct tgtagagcga gtcgcacttc gactccagcc tcctgcgggc ctccggcaac
     3781 gatcttgtgc ccagcccggc gccggcactg tagatccggg aacgcacttc gtcggaccca
     3841 agcgacgaaa gcaatgccag ctcggccagg ccaaatgcaa agacattggt gtagagggcc
     3901 ttcgacatgt tgcccactgc gggtgccggc tgtcgagact gccctcgctc cgcataccgc
     3961 acgatccggt ccacgcttcc atcgagcatc tccacagtca ggcttccgcc gtggcgtccc
     4021 ccccgttccg gagcgtagaa gcctcgcgaa gcctcgccct cgaaaccgaa cagaatgccg
     4081 cggatgaaag acatcaacgt ggttttgcca gcctcattgg caccagtgaa cagcacaacg
     4141 ccctggttgc cggaagcggt cccgctgttg cactcgaccc cgctgccgcc cgctatcaca
     4201 atgcgccggt cgtggaatat cccgaatccg tcaaggtaaa cttcgcgtat gcgcatggcc
     4261 tatgaatcag ccccctccag aagcaggtcg agcgcaagcg attccgcctt cgcaatgatc
     4321 tgcaggatat cctcatcgct aagctcgcga accaaccgac tcaaggcgcg atcggcgcga
     4381 ctgcccaatc tctgctcgag cgctccgcgg actgcctcca gacgggtcgg gtcggacctc
     4441 atgtcgtctg tcaggcacag taggtcggat atgagactgc caacggcctt gagcgcatcc
     4501 ctgtccacgt ccgggcctgt cagatcctgt acgcactcaa tccagtcaaa tcgatcgccg
     4561 ggaagtgaag attcgcgcgc agcggccagg agagcctccg gcccgtccat agccagctca
     4621 cggtggaccg gccctcgccc gcaaaggcgc actcggacag cacatgaccg catgttgctc
     4681 gacatcgcc
//
LOCUS       DAOTAC010000206         4655 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_15230_length_4655_cov_3.842174, whole genome shotgun sequence.
ACCESSION   DAOTAC010000206 DAOTAC010000000
VERSION     DAOTAC010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4655
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15230_length_4655_cov_3.842174"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="PLM74_08830"
     CDS             complement(<1..292)
                     /locus_tag="PLM74_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43215.1"
                     /translation="MQSEGERILTVPQEMGDVESPLVTGNEWVTLPDISPADAGIATV
                     NLVHMGCRGLVEWVGPEEPSGHAGLGRAPFLAPYIESGEGQSGKEAQTARAQN"
     gene            complement(297..1022)
                     /locus_tag="PLM74_08835"
     CDS             complement(297..1022)
                     /locus_tag="PLM74_08835"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HON43216.1"
                     /translation="MGKVALAVCAHPDDLEFFCGGSAVAMVREGWDVWLTIATDGDRG
                     THDPAVSRESLAAVRREESHKAGETIGLSGIVFLGKEDGELYADHSLRRDLARVYRQI
                     RPNRLITFDPWRRYELHPDHRAIGFASLDARLAARLPHYYSDLLRDGLEPCTIAEILL
                     FSTDAPDYYVDISSTFDTKLSAVGQHHSQWESIWGDTARSLRQEAESAGRRSGYAMAE
                     AFKRIFVPMGPVSADWHLKEEGC"
     gene            complement(1010..2167)
                     /locus_tag="PLM74_08840"
     CDS             complement(1010..2167)
                     /locus_tag="PLM74_08840"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HON43217.1"
                     /translation="MGSVTDGVKTRIASFVLARSSDPERISRRATWLRQADDRFRHRD
                     RPSKLRMGGPRPERLTCAAVQMKATLSHSGREYVDLVYRLAAKAAAAGADIVAFPEYS
                     GLPLLGMIPGMEKILAHADSLAQAVGPAGDVLEVFRFLGPYADRAYRAAFSSVSRELG
                     IYIVSGTMVLPDETGSRNAMRSLGYIFGPEGELCGKYHKTHLMPVESGWGIEPGDSMS
                     TVNIGGIIVAVPICMDATYFETFRIARLSGADLVIVPSANNEPYNLWYALRGVWPRVQ
                     ESQVYGIGASMVGTFAGQTFTGRSAILAPLELSPGGDGMLAQASTYDSEEIVIARIDI
                     PLLYKFRADNPLRFNLPLYRKYLPVMYRAALQARQPSAGQSSKEGNDTWER"
     gene            complement(2171..3091)
                     /locus_tag="PLM74_08845"
     CDS             complement(2171..3091)
                     /locus_tag="PLM74_08845"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HON43218.1"
                     /translation="MSRLACGLLQIDPSGFDTAHDFQVFLTAKISSLAQAGARLIVLP
                     AYSAMIGPALQLESEGRSPCNPFELAGSVGTDGFSAWMDFFAAAALSNRIYLCPGTFI
                     TPDDGEFEHRAPLFSPDGNLAMVQLQTHVSEAEAQAGMVVGADLAVVDVFSHKVGISI
                     GTDAWCPEVGRILALLGADTILAPTAVPAPYTVWRQTSGLWQIIQQNQVFGIEASLAG
                     RWLGTTYQGRSRVFGPVEVTPDGRGVLGEVTSESIEGEMIASLDLADLQTARRAFPIF
                     EHFNIGLYEARLADAYLVGHPARVRSEGRQ"
     gene            3252..>4655
                     /gene="aspS"
                     /locus_tag="PLM74_08850"
     CDS             3252..>4655
                     /gene="aspS"
                     /locus_tag="PLM74_08850"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HON43219.1"
                     /translation="MLRTHTCGELRASHAGQAVTLCGWAHSVRDHGGLVFITLRDRYG
                     FTQVVVEPGASQSAAECARGIHRESVLRVRGTVSRRPEGTWNSAIATGEVEVIASDIE
                     IMSDAAPILPVEVSEDKLANEEARLRYRYIDLRRHSLQANLHARHRAALAVRNWLETQ
                     DFIEVQTPLFVRSTPEGARDFVVPSRNFPGMFYALPQSPQLYKQLLMIAGFDRYFQLA
                     PCFRDEDQRADRQLVHTQIDLEMSFCDEEDVFTIVEGIISAAFMAVNGVEVKGPFLRL
                     SYDEAMERFGSDKPDLRFGMEMRDLTDLVSESEFSIFREACASGGRVRGLAATGCAHF
                     SRKETDELADIAKIYKAKGIITLKIEEGQLKGSAAKYLSEDAQRGIVERLQASDGDLV
                     IMTADNPEVSAVALGQVRRFLGRKLGLIKEGDYRLLWVTEFPLFEWNPDENRWEAKHH
                     IFTQPREQDLPILESDPA"
BASE COUNT          899 a         1389 c         1448 g          919 t
ORIGIN      
        1 tgttttgggc gcgggcggtt tgggcttctt taccgctttg gccttcgccg ctttcgatgt
       61 acggagcgag gaacggagcc cgcccaagcc ccgcgtgtcc cgacggttcc tccggaccaa
      121 cccactccac aagccccctg cagcccatgt gtaccaggtt gacagtggcg atcccggcat
      181 ctgccgggga aatatcgggg agcgttaccc attcgttgcc tgtaactagg ggcgactcga
      241 cgtctcccat ctcctggggg acagtgagga tccgttcgcc ttcagactgc attaggtcaa
      301 cagccctcct ctttgagatg ccagtcggcg cttaccgggc ccattgggac gaatatgcgc
      361 ttgaaggctt cggccatcgc atatccgctt ctgcgcccgg ccgactctgc ttcctgtcgc
      421 agcgagcggg cggtatcgcc ccatattgac tcccattggc tgtggtgctg gcccaccgca
      481 gagagctttg tgtcgaacgt gcttgagatg tcgacgtagt agtcgggcgc gtccgtactg
      541 aagagcagga tctccgctat ggtacacggc tcaagcccat cgcggagaag gtcggagtag
      601 taatggggca atctggccgc gagccttgcg tcgaggcttg cgaaaccgat ggcgcggtga
      661 tcggggtgta gctcatatcg gcgccacggg tcgaaggtga tcagccggtt cgggcgaatc
      721 tgcctgtaca cgcgggccag gtcccggcgg agcgaatggt ctgcgtacag ctccccgtct
      781 tcttttccga gaaagactat gcctgacagc cctatggtct cccccgcctt gtgagactcc
      841 tctctcctga cggcggccag tgactcacgg gagacggcag ggtcgtgcgt accccggtcg
      901 ccgtcggtgg cgatggtcag ccagacgtcc catccttccc gcaccatggc gactgccgag
      961 ccgccgcaga agaactccag gtcgtcgggg tgagcgcaca cggcaagtgc tacctttccc
     1021 aagtgtcatt cccctctttc gacgactgcc cagcgctggg ctgccgggct tgtagcgctg
     1081 ctcggtacat aaccggcaag tacttgcggt acagcggaag gttgaaccgc agggggttgt
     1141 cggccctgaa cttgtagagc agcggaatgt cgattctagc gatgactatc tcttcactgt
     1201 cgtaagtgct ggcctgggcc agcatgccgt cgccgcctgg agagagctcc aggggagcca
     1261 ggatcgcgct ccggccggtg aaggtctgtc ctgcgaatgt acctaccatc gacgccccga
     1321 tgccgtacac ctgcgattcc tgcacccggg gccatactcc gcggagcgcg taccacaggt
     1381 tgtagggctc attgttggct gacgggacga tgactagatc agctccggaa aggcgtgcta
     1441 tccggaaagt ctcgaagtag gtcgcgtcca tgcatatggg aacggctacg atgattcccc
     1501 cgatgttgac ggtggacatc gagtcgcctg gctctatgcc ccagcccgat tccacgggca
     1561 tgagatgggt tttgtggtat ttgccgcata gttcgccctc ggggccgaat atgtagccca
     1621 gcgagcgcat ggcattacgg ctcccggtct catccggcag caccatggtg cctgacacaa
     1681 tgtagatgcc cagttcacgc gagaccgagg agaatgccgc gcgatacgcc ctgtcggcat
     1741 acggccccag aaacctgaac acttccagaa catctccggc gggccctaca gcttgagcca
     1801 gcgagtcggc atgagcaagg atcttctcca tgcctggtat catgcccagg aggggcagac
     1861 cgctgtactc ggggaaggcc acgatatcgg cgccggccgc ggcggccttc gcagctagcc
     1921 tgtatacgag atcgacatat tcgcgaccgg agtgcgacag cgtagccttc atctgtacag
     1981 ctgcacacgt caggcgctcc ggccttggcc cacccattcg cagcttggac ggtcggtcgc
     2041 gatgcctgaa cctgtcgtcg gcctgcctga gccaggtggc ccgccgggag atgcgctcgg
     2101 ggtcggacga gcgggccagc acaaagctgg cgatcctcgt tttcacgcca tctgtcactg
     2161 atcccacccg ttactgcctc ccctcggacc tgacgcgcgc tgggtgccct acgagatatg
     2221 catcggccag tcgcgcctcg tacagaccta tgttgaagtg ttcgaatatg ggaaatgcgc
     2281 gtcgggcggt ttgcaggtcg gccagatcga gtgaggcaat catctcgccc tcgattgact
     2341 cgctcgtgac ctcgccgagc acgccgcgtc catcgggcgt cacttcgacc gggccgaaca
     2401 cgcgcgagcg gccctggtag gtcgtgccga gccatcgtcc tgcaagggag gcttcgatgc
     2461 caaaaacctg gttttgctgg atgatttgcc atagcccgct agtctggcgc cagacggtat
     2521 agggagccgg tacagcggtc ggagccagga tcgtatcggc acccagaagc gccagaatgc
     2581 ggccgacctc cgggcaccac gcatcagtgc ctatggagat gcccaccttg tggctgaaga
     2641 cgtctaccac cgccaggtca gctcctacca ccatccctgc ctgggcctcg gcctctgata
     2701 catgggtctg aagttggacc atggccagat tgccgtcggg tgagaaaagc ggagcgcgat
     2761 gttcgaactc gccgtcgtcg ggtgtgatga atgttccagg gcagaggtat atcctgttgg
     2821 agagtgcggc ggcggcgaag aagtccatcc acgcggaaaa cccatctgta ccgacagatc
     2881 cggcaagctc gaacgggttg cacggcgaac gtccttccga ttcaagctgt agtgctggcc
     2941 cgatcattgc ggagtacgcc ggcaacacga tcaggcgcgc ccccgcctgc gcaagcgatg
     3001 atatcttcgc ggtcaggaag acctggaaat catgagcggt gtcgaacccg gaggggtcta
     3061 tctgaagaag accacaggct agacgagaca tagaggtcac ctctctttgg agattacaca
     3121 tttttcgccc cgttcctgca gtgtcgaggt acaagtgtcg aagtgtcgag gtggaagttg
     3181 cactaagaca cgttgcaggg ctataattgg gttaattctc tcttggattt attactatcg
     3241 aggtgagttt cgtgcttcgc acacacactt gcggagaatt gagagcctcc cacgcgggcc
     3301 aagcagttac cttgtgcggt tgggcccatt cagtgcgcga ccatggtggt ctagtcttca
     3361 tcaccttgcg cgataggtat ggcttcaccc aggttgtggt ggagcccggc gcgtcgcaga
     3421 gcgcggctga atgcgcgcgc ggcatacacc gcgaaagcgt gctccgcgtc cgaggcacgg
     3481 tgtcgcgtcg gcccgaaggc acctggaaca gcgcaatcgc aacaggagag gtcgaggtaa
     3541 tcgccagcga catcgagatc atgagcgacg cagcgcccat cttgcccgtg gaggtatccg
     3601 aggacaagct ggctaacgaa gaagccaggc tgagatacag gtacatcgat ctcagacgtc
     3661 attctctgca ggccaacttg cacgcgcgtc atcgggcggc cctggccgtg cgcaactggc
     3721 tcgaaacgca ggacttcatc gaggtccaga cgcctctatt cgtccgcagc acgcccgagg
     3781 gggcccgaga cttcgtggtg cccagccgga acttcccggg catgttctat gcgcttcccc
     3841 agagccctca gctgtataag cagttgctga tgatcgcggg cttcgatcgc tacttccagc
     3901 tggccccctg cttccgcgac gaggaccaac gggctgacag gcagctggtt cacacccaga
     3961 tcgatctgga gatgagcttc tgcgatgaag aagacgtgtt caccatagtc gaggggatca
     4021 tctccgctgc gtttatggcc gtgaacggcg tagaagtgaa gggcccattc ctaaggctca
     4081 gctacgacga agctatggag cggttcggct cggacaagcc cgatctcagg ttcggcatgg
     4141 agatgcgcga tctcacggat ctcgtgagcg agtcggagtt tagcatcttc cgcgaagcat
     4201 gcgcttcggg cggccgtgtg cgcggtttgg ccgcgacagg atgtgcccac ttcagccgga
     4261 aggagaccga tgaactagcg gacattgcca agatatacaa ggcaaagggc atcatcacgc
     4321 tcaagatcga ggaggggcag ctcaagggga gtgccgccaa gtatctctcg gaagatgccc
     4381 agcgcgggat cgtggagcga ttgcaggcgt cagatggcga cctcgtcatc atgaccgcag
     4441 ataacccgga agtgtcagca gtcgcgctcg gccaggtcag gcgttttctc gggcgcaagc
     4501 tcgggctgat caaggagggc gactacaggc ttctgtgggt gacggagttt ccgctatttg
     4561 agtggaaccc cgacgagaat cgctgggagg ccaagcatca catctttacc caacctcgcg
     4621 agcaggactt gcccatactg gagtcggatc cggca
//
LOCUS       DAOTAC010000207         4653 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_15238_length_4653_cov_5.424532, whole genome shotgun sequence.
ACCESSION   DAOTAC010000207 DAOTAC010000000
VERSION     DAOTAC010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4653
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15238_length_4653_cov_5.424532"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1207)
                     /locus_tag="PLM74_08855"
     CDS             complement(<1..1207)
                     /locus_tag="PLM74_08855"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HON43220.1"
                     /translation="MNMNILDMMDRTQKGFERRLRTFQIIALALFIVLAGRLWQLQVM
                     RGDYFKSRSTANRIAVVPISAPRGLMVDRNGEVLATSRMAYTVSAMPQEFRDRESEVE
                     LLSQLLGMTREEIEAKIAGQTEGKYGIPYQPARLVEDAPPDIIMKVSEHRVDLPGVII
                     EEEPYRSYPHGTLAGTIMGYVGQISQDELKQLGDRGYRGRDRIGKTGLEREFEEYLRG
                     QDGVTQVEVDSLSRPRGTVGYTEPVGGATLVLTIDARVQQAAEDALRAQLASLATGKY
                     RNARAGAAVAIDPRTGEILALASEPGYDPGWFVPKISEENWRRVSTGVSALFNRAVSG
                     AYPPGSTFKPFTAMAALESGQVSLDERFLCTPSVSSRYYGKKCSVWSNGRTHGSQNLF
                     QGMSNSCNIV"
     gene            complement(1213..1761)
                     /gene="mreD"
                     /locus_tag="PLM74_08860"
     CDS             complement(1213..1761)
                     /gene="mreD"
                     /locus_tag="PLM74_08860"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HON43221.1"
                     /translation="MKQARVIVALFVSVLLQTAVIPALLPGVVVPDFVFCLSAVVAIA
                     AGGRPGVALAAVGGLIKDGLTGAFIGANAGADMVAAVLIGFVEPQLFKESLLTPAVVI
                     GAATWAREAVYMFSIGSFGARVDMQRAICVVAPWLTALNICAGVLIYWWIYLVPRERQ
                     RTLIQAEPAASMELRPRGPGRS"
     gene            complement(1758..2594)
                     /gene="mreC"
                     /locus_tag="PLM74_08865"
     CDS             complement(1758..2594)
                     /gene="mreC"
                     /locus_tag="PLM74_08865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HON43222.1"
                     /translation="MNKLLSRRRAIVTCVSVILLVTLIYSTARDRERLSAAEHVLREV
                     FAPVARGANYVSRAVSLWVSFVASIGRMKRENEALSAEVDQLRSEVVQLSEAARENAR
                     LSSLLELKSSLDGRAVAARVVYRDPASWLETAVIDKGSSQGVGTGMAVVGPRGVIGRT
                     TSVTAHTATVLFAIDPRSAIGGIDSRSRDLVIAEGIGDGSGLLRVRPIGADCDIVAGD
                     VIVTSGMGGIYPKGHIVADVISVEQDKYGVSTTAIARPRVDFQRLEEVLVLIDPTRGA
                     AR"
     gene            complement(2622..3662)
                     /locus_tag="PLM74_08870"
     CDS             complement(2622..3662)
                     /locus_tag="PLM74_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HON43223.1"
                     /translation="MAFASLFSGWSRDMGIDLGTANTLVFVRGKGIVLQEPSVIAIDH
                     NSQEILAVGYDAKKMVGRTPASIVAVRPLRDGVIADLDVTVTMLRHFIDKASRRHGVF
                     KPRVVIGVPSGVTQVERRVIHDSAMQAGAKEACLVEEPMAAAIGAGLPVEDPTGNMVV
                     DIGGGTTEVAVISLGGIVASKSITTAGDKMDEAIVNYMKRNYNLMIGERTAEEIKKTV
                     GSAYPIQQEETMEARGRDIVNGLPKTVTISSSEIQQALSDTVSEIVNAVRSTLEKTPP
                     ELSADVIDKGIAMTGGGSMLRGLDRLLSDATGMPAYLTEDPLTCVVRGAGLVVEHLDT
                     YRRVLVSSGRLK"
     gene            complement(3721..4428)
                     /gene="radC"
                     /locus_tag="PLM74_08875"
     CDS             complement(3721..4428)
                     /gene="radC"
                     /locus_tag="PLM74_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017692611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HON43224.1"
                     /translation="MNNGRRGIREMPPEERPTERFLEFGPEAISTAELLAIIIRCGRR
                     GESAVDVASRVLSYFGSARRLATASVAELSDIPGIGSVRSVQIQAAIELGRRVLLSES
                     ELRPAAANPHDVADLLMPGMRYLEKEEFRAVFLDARNRVIDTATISVGTLNSSIVHPR
                     EVFRAAIKAGSAALILVHNHPSGDPSPSPEDLAITKRLVRAGSMLGIEVLDHIIIGDN
                     VYVSLREKGLLGSAQQQ"
     gene            complement(4514..>4653)
                     /locus_tag="PLM74_08880"
     CDS             complement(4514..>4653)
                     /locus_tag="PLM74_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HON43225.1"
                     /translation="KAGAYAIQGLGSLLVTGISGDYNCVVGLPLCRLAMMLSEFGVDT
                     L"
BASE COUNT          923 a         1548 c         1265 g          917 t
ORIGIN      
        1 agactatgtt gcacgagttg gacatgccct gaaacaggtt ctggctgccg tgtgtgcgcc
       61 cgttggacca aaccgagcac ttcttgccgt agtagcgtga cgacacagac ggtgtgcaca
      121 aaaacctctc gtccaggctg acctgccccg attccaacgc tgccatggcc gtgaagggct
      181 tgaacgtgga gcctggcgga taggcgcctg acacagccct gttgaatagg gcggacacgc
      241 ctgtgctcac gcggcgccag ttctcctcgg agatcttcgg cacgaaccag cccggatcat
      301 atcccggctc tgaagcgagc gccagaatct cgccggtgcg cggatcgatg gcgactgcgg
      361 cgccggctcg ggcgttcctg tacttcccgg tcgcgagcga cgcaagttgc gcgcgaagcg
      421 cgtcctcggc ggcctgctgc acccgggcgt cgatcgttag aaccagcgtg gcgccgccca
      481 caggctctgt gtagcctacc gtgccccttg gtcgcgatag cgagtctact tcgacctgtg
      541 tcacaccgtc ctgaccgcgc aagtactcct cgaattcccg ttccagccct gtcttgccta
      601 tgcggtcgcg tccgcggtag ccgcggtcgc cgagctgttt cagctcatcc tggctgatct
      661 ggcctacata tcccatgatc gtgccggcca gcgtcccgtg aggatagctc ctgtacggtt
      721 cctcctcgat aatcaccccg ggcaggtcca cccggtgctc ggagactttc atgatgatgt
      781 cgggaggcgc atcctccaca aggcgagcag gttgatacgg aataccgtac ttgccctcag
      841 tctgacctgc gatcttcgcc tcgatctcct cgcgcgtcat gcccagaagc tgcgagagca
      901 actccacctc gctctcccta tcgcggaact cctggggcat cgcagagacg gtatatgcca
      961 tcctgctcgt tgcgagcacc tcaccgtttc tgtccaccat gaggccacgc ggggcggaaa
     1021 tgggcactac tgcgatcctg ttagccgttg agcggctctt gaagtagtcg ccgcgcatga
     1081 cctgcagctg ccacagcctt cccgcaagca caatgaacaa ggccagcgca atgatttgga
     1141 acgtcctcag ccggcgctcg aaacccttct gggtgcggtc catcatgtcc aagatgttca
     1201 tattcatggc gatcagctcc gcccgggccc gcgagggcgc aactccatac tggccgccgg
     1261 ctctgcctgg atgagagtgc gttgtcgctc ccgcggaaca aggtaaatcc accagtaaat
     1321 caaaacgccc gcgcagatat tgagcgcggt cagccatggc gccactacac atatcgcccg
     1381 ctgcatgtct acgcgtgcgc cgaatgatcc tatggaaaac atgtaaacag cctcgcgcgc
     1441 ccaggtggcc gcgcctatca ctacggccgg cgtaagcagg ctctccttga acaactgggg
     1501 ttcgacaaat ccgattagga ctgccgcgac catatcggcc ccggcgttag cgccgatgaa
     1561 agcccctgtc agcccgtcct tgatcagccc gcccacagcg gcaagagcca cgcccggacg
     1621 gccgcccgcc gctattgcca cgaccgctga taggcaaaag acaaagtcgg gaaccacgac
     1681 accgggtaga agtgccggaa ttacggcggt ctgcaacagc acagagacga acaacgcgac
     1741 aataacccga gcctgcttca tcgcgccgcc ccccgcgtcg ggtcgatgag caccagcact
     1801 tcctcaaggc gctggaaatc cactcgcggc ctggctatgg ccgtcgtcga taccccgtat
     1861 ttgtcctgct ccacgctgat cacatccgcg acgatatgcc ctttgggata gatgcccccc
     1921 atgcccgatg tgacgataac atcgcctgcc acaatgtcgc aatcggcgcc tatcgggcgc
     1981 acccgaagaa gccccgatcc gtcgcctatt ccttctgcta tgaccagatc gcgcgacctg
     2041 gagtcgatcc cgccgatcgc gctgcgcgga tcgatcgcaa acaacaccgt ggccgtatgc
     2101 gcagtaacag acgtcgtgcg cccgattacg ccccttgggc ccacgaccgc catgccggtc
     2161 cctacgcctt gactggatcc cttgtcgatc acagcagtct ccaaccagct ggcaggatcg
     2221 cgatacacaa cacgagccgc gaccgcacgc ccgtcaagcg acgatttcag ctccagaagg
     2281 ctgctcagcc tagcattttc cctggctgct tcggaaagtt gcaccacctc ggagcggagt
     2341 tggtcgacct cggcgcttaa ggcttcgttc tcccgcttca tccgaccgat tgacgccaca
     2401 aacgagaccc ataacgaaac ggccctgctc acgtagttgg cgccacgggc aacgggcgcg
     2461 aagacctcgc gcagcacgtg ctcggccgcc gacagccttt cgcggtcgcg ggccgtggaa
     2521 tatatgagtg taactaggag gatcacactc acgcaagtga cgatggctct cctcctcgat
     2581 agcagcttgt tcacaggagc gaccctcccg ggcctagcac cctacttgag cctgcctgac
     2641 gacacaagca cccgccgata agtgtcgaga tgctccacca cgaggcccgc gcccctcaca
     2701 acgcaggtga gagggtcctc tgttaggtac gccggcatcc ctgtagcatc cgaaagcagc
     2761 ctatccaggc ctctcagcat ggatccaccg cctgtcatgg ctattccctt gtcgatgacg
     2821 tcggccgaca gctcgggcgg cgtcttctcc agcgtgctgc ggacagcgtt cactatctca
     2881 gaaaccgtgt ccgagagagc ctgctgtatc tcggagctgg atatcgtgac ggtcttgggc
     2941 agcccgttga cgatgtcgcg cccacgggcc tccatcgtct cttcctgctg gatcgggtat
     3001 gccgagccca ccgtcttctt tatctcttca gcggttcgtt cgcctatcat caggttgtag
     3061 ttgcgcttca tgtaattcac aatggcctca tccatcttgt cgccagcggt ggtgatggac
     3121 ttggatgcca ctatgcctcc gagcgagatc acggcaacct cagtggtgcc cccgccgata
     3181 tccacgacca tgttacctgt ggggtcttca acaggaagcc ccgcgccgat ggcggcggcc
     3241 ataggctcct cgaccagaca agcttcttta gcgccggcct gcatggccga atcatgtatc
     3301 acacggcgtt caacctgggt aacgcctgac ggcacaccta tgacgaccct gggcttgaac
     3361 acaccgtggc gccgcgaagc cttatcaatg aaatgcctga gcatcgtaac tgtaacgtca
     3421 agatcggcga tgaccccgtc ccttagagga cgtaccgcaa cgatactcgc aggggttctg
     3481 cccaccatct ttttggcgtc gtaaccgacg gccaagatct cctggctatt gtgatcgatg
     3541 gcaatgaccg agggttcctg aagcactatc cctttgcccc ttacaaagac aagtgtattg
     3601 gcagtaccca ggtcgatgcc catatccctc gaccaaccgc tgaagagaga agcaaaggcc
     3661 atgacggatg gctcccttct aaacaggatt tgtcactgca gtgtcattgc agtctcagcg
     3721 ctattgttgc tgcgccgaac cgagcaagcc cttttcgcgc aggctcacat atacattgtc
     3781 gccaatgatt atgtggtcaa gtacttctat cccaagcatc gatcccgccc gtaccaggcg
     3841 tttagtgata gccagatcct cggggctcgg gctgggatcg ccgctcgggt ggttatgaac
     3901 aagtatcaaa gcagcgctgc ccgcctttat cgccgcacga aacacctctc gaggatgcac
     3961 gatggacgag ttgagcgtgc ccaccgatat cgtcgctgtg tctatcaccc tgttccgggc
     4021 atccaggaag accgcccgga actcttcctt ctcaagataa cgcatgcccg gcatcaggag
     4081 gtcggccaca tcatgcggat tggccgccgc tggccttagc tcgctctcag acaggagcac
     4141 tcttcgcccc agctcaatgg ctgcctgtat ctgtacagac cgtaccgatc ctatccccgg
     4201 gatatccgaa agttctgcca cactggccgt agccagcctt cgcgcgctgc cgaagtaaga
     4261 cagcacacgc gatgccacat caacagcgct ttcgccacgc cgaccgcatc tgatgatgat
     4321 cgccagtagc tctgcagtgg aaatggcctc cggcccgaac tccaagaatc gctccgtggg
     4381 gcgttcctca ggtggcatct cgcgaatgcc gcgtctgccg ttattcatgg tcacacctcg
     4441 cctgcggctt attgcaagcg cggccgcggg ttggccaacc atgttaggcg gcgagcgaca
     4501 agacgcctac gacctacagc gtatccacac cgaactccga tagcatcatg gcgagtctgc
     4561 acagaggcag ccccaccacg cagttgtaat cgccgctgat ccccgtgacg agcaggctgc
     4621 ccagcccctg gatggcgtag gcgccggcct tgt
//
LOCUS       DAOTAC010000208         4647 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_15275_length_4647_cov_4.508711, whole genome shotgun sequence.
ACCESSION   DAOTAC010000208 DAOTAC010000000
VERSION     DAOTAC010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4647
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15275_length_4647_cov_4.508711"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /locus_tag="PLM74_08885"
     CDS             complement(<1..850)
                     /locus_tag="PLM74_08885"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HON43226.1"
                     /translation="MKAAVYHGPGDLRVEQIPKPEAMPGGALLEVSYCGLCGTDIKTY
                     RRGHHMFTPPCVLGHEVVGRIIEMKDPHASVSVKEGDFVAVAPYVPCFVCPLCARGRH
                     ELCRHKDWIDGGFAEYVTVPAGVLEKGTFRVPDGLDPRVAALAEPLACCLNAVTDSGV
                     HMGDTVLVIGAGPMGMMMLESCKAAGATKLLVSEPDEFRRSQAERRGAQAIDPGRSSV
                     AEWVNDVTCGAGVDVVFVCVGAAEAVGEAMAAAGPGGVVNVFGGLAGGVTISVDARRV
                     HYDEVTL"
     gene            complement(847..1611)
                     /locus_tag="PLM74_08890"
     CDS             complement(847..1611)
                     /locus_tag="PLM74_08890"
                     /inference="COORDINATES: protein motif:HMM:NF013916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HON43227.1"
                     /translation="MTGVDVRLRRIFKDGKAVVCPLDFGGFMGPVQGIDDPSRIVGEV
                     VAGGADAILVNPGMARSEWESYAGRAGLIVRITGGSTKFSPNPAFHTLVCSVDEACAI
                     GADAVCVMVLVGSEQEQAMFEIMGDVVSDAHILGIPVLAEVIPADPAHNFDPEWISVC
                     ARVGYELGADFIKTYFTDDGFANIVRACRAPIIIAGGPKVADPNTVVRQAMQCGAAGI
                     AFGRNVFQSVDPRAKVRELHAIVHGPGAPCGEVRQA"
     gene            complement(1598..2722)
                     /locus_tag="PLM74_08895"
     CDS             complement(1598..2722)
                     /locus_tag="PLM74_08895"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HON43228.1"
                     /translation="MSDCRDSLLERMAGSLAGVAIGDAMGMPCEFLTRGQIREKYAWL
                     NWFVAPDASHFHHGMRAGRITDDTEQTIALIGALDKHSRITPETAAEAYLKWADDCNA
                     WQSTVMGPSTRRALERLKAGEDPRTTGSSGDTVGAAMRVAPIGLVNAGRLAEAAEECY
                     MSCLPTHGVSIAIGGACAVACAVACAAVCSVGREVAGATTGVITGATTGATTCEQAAT
                     AVADTGVIGEILDAAVWGAEYGEARGAAWAGASVVARIRLARRIVAESRCAGEALDAL
                     YETCGVGMLPTELVPTAMGIVALYGADCAKAVEAAVNMGGDADTLASIVGAVTGALHG
                     IGGFPTEWVRTVEDVNGISICELARRLVCVRERRSVHDRS"
     gene            complement(2719..3714)
                     /locus_tag="PLM74_08900"
     CDS             complement(2719..3714)
                     /locus_tag="PLM74_08900"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HON43229.1"
                     /translation="MGGKGRCGGSGKCVVCLGACAMDLIIRVPRLPEADEMVFAEGEP
                     GLFPGGSTANIAAGIARIGVASRFVGKVGDDPNGRALREAFEKDGVDVRWLVADSAGR
                     TAQTVIAVDPSGSRVIYSLGGTALLESVEEFTGDILDGAAALYVGEAFPDVARRAMQQ
                     ARSVGAQVVYGPGGAASWIGMENLAELMRLADFVLMSRAELRAITECDEPDAGARLLL
                     ERGVPSVVATLGEQGAACFSRRPEHVADIAWAPSFNVDVVDTTGAGDAFASGFISSLV
                     EGRPTAECLRRGNACAAMAIGRVGAREALPTSEELEMFLAARGGAGLPEKDGVEV"
     gene            complement(3715..4557)
                     /locus_tag="PLM74_08905"
     CDS             complement(3715..4557)
                     /locus_tag="PLM74_08905"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HON43230.1"
                     /translation="MFRVIVEYESKSSAVHRLDPRVKFARMLAAIVISVMWTHPVYLI
                     VLALAVVGFGMLAGFPWSKIRGVLSFMIIVTAVITLVQGATYVPKTVTLDEPARVLFH
                     LIPGWIPRVGPACPIRVGGFLYGVGMALKVVTVLVVVAIFGFTTSPSEIVQIIARVPF
                     MPYQVGFVMSTAWKFVPVVQMQMRTLMDAHRSKGVDFEQGTFAEKIRKTSGIVLPLFA
                     NSLSMADTMALAMESRAFGSSRKFTFIRPYRMEPFDWVVLIGSLGAMVASIVLLAVFR
                     LGAL"
BASE COUNT          928 a         1683 c         1224 g          812 t
ORIGIN      
        1 tcagagtcac ctcatcatag tgcactcgtc ttgcatctac ggagattgtc acgccacctg
       61 caagcccgcc gaatacgttc acgacgccgc caggccccgc cgccgccatg gcctcgccca
      121 cagcttcggc ggcgccgacg catacgaaca ctacatctac gcccgcgccg caggtaacat
      181 cgttgaccca ctccgcaacg ctactccgcc caggatcgat cgcctgcgct ccgcgccgct
      241 cagcctgcga tctcctgaat tcatcgggct cgctcacgag caacttggta gccccggcgg
      301 ccttgcacga ctccagcatc atcatgccca taggcccggc gccgattacc agcacggtat
      361 cgcccatgtg cacgcctgag tcagtgacgg cattcaaaca gcaagccaga ggttctgcca
      421 gcgctgccac tctcggatcc agcccatcag gcaccctgaa cgtgcccttc tcaagaaccc
      481 cggcaggcac ggtcacgtat tccgcaaacc cgccgtcaat ccaatcctta tgcctgcata
      541 gctcatggcg gccgcgcgcg cacagcgggc acacaaagca tggcacgtac ggggcaaccg
      601 ccacgaagtc gccttccttg acactcaccg acgcatgcgg gtccttcatc tcgataatcc
      661 gccccaccac ctcgtgaccc agaacgcaag ggggagtgaa catatggtga ccccgccggt
      721 atgtcttgat atccgtaccg cacaggccgc aatagctcac ctcaagcaat gcgccgcccg
      781 gcatggcctc aggttttggg atctgctcta cgcgcaaatc gcccggccca tgataaacgg
      841 ctgccttcaa gcttgtcgca cctccccgca gggcgcccca ggtccgtgga cgattgcgtg
      901 cagctcacgc accttggccc tgggatctac cgactggaac acgttgcgtc caaaagcgat
      961 gccggccgct ccgcattgca tggcctggcg aaccacggta ttgggatcgg caactttggg
     1021 cccgcctgca atgataatcg gcgcccggca cgcccgcacg atgttggcga aaccgtcatc
     1081 agtgaagtaa gtcttgatga aatccgctcc cagctcatag ccgacgcggg cgcacacgga
     1141 tatccattcg gggtcgaaat tgtgtgcggg gtctgcagga atcacctcgg ccagcaccgg
     1201 tatgccaagg atgtgggcat cggatacaac atcccccatg atctcgaaca tcgcttgttc
     1261 ctgctctgag ccgaccagaa ccatgacaca cacagcgtcc gcgccaattg cgcatgcctc
     1321 gtccacgctg caaaccaggg tgtggaaggc cggattgggg ctgaacttgg tagacccccc
     1381 tgtgatcctg actatgagac cggcacgacc ggcgtagctt tcccactcgc tcctagccat
     1441 tccggggttg accaggattg cgtccgctcc gcccgctacc acctcgccca cgatccttga
     1501 ggggtcatct atgccctgca caggccccat gaacccgccg aagtccagcg ggcacactac
     1561 ggcctttccg tctttgaata tccgtcggag cctgacatca actcctgtca tgaacagatc
     1621 tcctttctct cacacaaacc agtcgccgag cgagttcaca aatgctaatg ccgttgacat
     1681 cctctacggt cctcacccat tcggtcggga accccccgat tccgtgcaga gctccggtca
     1741 cagcgcctac aatggacgca agagtgtcgg cgtcgccccc catattgacc gccgcttcca
     1801 cggccttcgc acagtccgcg ccatagagcg ccacaatccc catggccgtc ggaaccagct
     1861 cagttgggag catccccacc ccacaggtct catacagcgc atccagcgcc tcgcctgcac
     1921 accggctctc ggccacaatc cgccgcgcca gccggattcg ggcgacaacc gatgcccccg
     1981 cccaagccgc gccgcgtgcc tcaccatact cagcgcccca caccgccgcg tcgagtatct
     2041 cgccgatcac gcctgtgtcg gcgactgcag tcgctgcctg ctcgcaagta gtcgcaccgg
     2101 tagtcgcacc ggtaatcaca ccggtagtcg caccggcaac ctcacggcct accgagcaga
     2161 ccgccgcgca ggccactgca caggctactg cgcatgcgcc ccctatggcg atgctcaccc
     2221 cgtgagtcgg taggcacgac atgtaacact cctcggctgc ttcagcaaga cggcccgcgt
     2281 tcaccaggcc aatcggagcc actcgcatag cagcgcccac ggtgtcgcct gaagagcctg
     2341 tcgtgcgcgg gtcctcgccg gccttcagac gttccaaggc ccgcctagtg ctcggcccca
     2401 ttaccgtcga ttgccaagca ttgcagtcgt cagcccactt caggtaggcc tccgccgccg
     2461 tctccggagt tatccgactg tgcttatcaa gcgccccaat gagagcgata gtctgctccg
     2521 tgtcatcggt aatccgcccg gcacgcatcc catggtggaa atggctcgcg tccggcgcga
     2581 caaaccagtt cagccaggcg tatttctctc gtatttgccc tcgcgtcagg aactcgcacg
     2641 gcatacccat ggcatcccct atggctactc cggcgaggct tcctgccatc ctttccagta
     2701 gactatctcg gcagtcgctc atacctctac tccatctttt tctggcagcc cggcgccgcc
     2761 gcgggccgcg agaaacatct caagctcttc cgaagtgggc agggcttcac gcgctccaac
     2821 gcgcccgatg gccatggccg cgcacgcgtt gcctcgtctc aggcactcgg ccgtcggtcg
     2881 cccctctacc agggacgata tgaacccgga cgcaaacgcg tccccggcgc ccgtggtgtc
     2941 aacaacatcc acgttgaacg aaggcgccca cgctatgtcg gccacatgct caggtcgccg
     3001 gctgaagcag gctgccccct gttcgcccaa ggtcgcgaca acgcttggca cgccgcgctc
     3061 caagagcaat cgtgcacccg cgtccggctc atcgcactcg gtaatagcgc gcagttcggc
     3121 ccggctcatg agcacgaagt cggccaaccg catcaactca gccaggttct ccatgccgat
     3181 ccaggacgcc gcgccgcccg ggccgtatac gacctgggcg cccacactgc gcgcctgctg
     3241 catggctctg cgcgcaacat ccggaaaggc ttcccccaca tatagcgccg ccgccccatc
     3301 caagatgtcg ccggtgaact cctccaccga ctcgagcaaa gccgtcccgc caagggagta
     3361 gatcactcgg cttcccgaag gatctacggc gatcactgtc tgcgcggtgc gccccgccga
     3421 gtccgccaca agccacctga cgtcgactcc gtccttctca aatgcctcgc gaagcgcgcg
     3481 accgttcggg tcgtcaccca ccttgccgac gaaccttgac gccaccccga tgcgggcgat
     3541 accagcggcg atgttggctg tggaccctcc tgggaacagc ccgggttctc cctctgcgaa
     3601 caccatctcg tcagcctcgg gcaggcgcgg gacccggatg atcaggtcca tggcacacgc
     3661 tccaaggcat acaacgcact tgccgctacc gccgcaccta cccttcccgc ccatttacag
     3721 cgcccccagc cggaacacgg ccagcagcac gatgctggca accattgccc caagcgagcc
     3781 aatgagaacc acccagtcga acggttccat gcgatatggt cggatgaagg tgaacttcct
     3841 gctggagccg aaggctctgg actccattgc taaggccatg gtgtcggcca tcgacagtga
     3901 gttggcgaac agcggcagaa cgattccaga cgtcttcctg atcttctccg cgaaggttcc
     3961 ctgttcgaag tcgacgccct tggagcggtg ggcatccatg agcgttctca tctgcatttg
     4021 cactaccggg acgaacttcc acgctgtaga catgacgaag cccacctggt aaggcatgaa
     4081 gggcaccctc gcgattatct gcactatctc agacggagac gtggtgaacc cgaatatcgc
     4141 cactaccacc agaaccgtga ctaccttgag cgccatgccg accccgtaca gaaatccgcc
     4201 cacccggatg gggcacgccg gtcctacgcg cggaatccaa cccgggatca gatggaacag
     4261 cacgcgtgca ggctcatcca gcgtcacagt cttggggacg tatgtagcgc cctgaaccag
     4321 ggtgattacg gctgtgacta taatcatgaa agacaacacg ccgcggatct tgctccacgg
     4381 aaacccggca agcatgccga agcccaccac ggccaaggcc aaaacgatca ggtagacagg
     4441 gtgcgtccac atcacgctga tcactatggc ggcaagcatc cgcgcgaact tgactcgcgg
     4501 atccaggcga tgcaccgccg aggacttgct ttcgtactct acaatcactc tgaacacacc
     4561 cccgcggctt gagatttggc ggcgagaagc ctggccgcgg cctcagccgg ggtcaagagt
     4621 acctcgcccc acagcgcctg cgacaga
//
LOCUS       DAOTAC010000209         4616 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_15368_length_4616_cov_4.258277, whole genome shotgun sequence.
ACCESSION   DAOTAC010000209 DAOTAC010000000
VERSION     DAOTAC010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4616
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15368_length_4616_cov_4.258277"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /locus_tag="PLM74_08910"
     CDS             complement(<1..750)
                     /locus_tag="PLM74_08910"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HON43231.1"
                     /translation="MTATPVHQRKRPSYTALGFLVPSLAILGIVVLIPLLYVIYLSFT
                     DYSFIAPTQSRFVGIRNYIDALWQRDFRNSLWVTTVFTVATVGLETVFGLIVALALRT
                     RQRYSRAFFSVYLMPTIITPISTGLVWAYILHPTLGVFNYLLGLLGTPTRAWLGEGGT
                     ALLSVCLVDVWEWTPLVALMLYAGLVSLPQEPVEAAHVDGATRWQVFRYVTMPLIRPV
                     ALGAVLIRALDALKTFDIVYVLTSGGPSNRTE"
     gene            complement(832..2136)
                     /locus_tag="PLM74_08915"
     CDS             complement(832..2136)
                     /locus_tag="PLM74_08915"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HON43232.1"
                     /translation="MKSRAILARALTMALVFAMALSLVTGVQAAKEKSITILLETVPD
                     SEYVIKLIPEFEKATGIKVNVEKTTFMVMRDKLMTNVLSGRGQYDVIVVDAPFWTGQF
                     VNGRWIIPLDDYIKETPSVNMDVYYPSMLDLAGVFDGVTYMLPFYQYNMVMLTRSDIV
                     DDPKIRKEYKAKFGKELDLDNLSVEEYVQVAKFLTRDTNGDGKVDIWGSIMTPKKSDT
                     YCDWANYFFGLGGDFYTNKKVTINSQAGVKALNLYMDTLKNAAPAGSMGFSFDDALNA
                     MAQGQGATMVTFNWMLGPLNDPAKSKVAGKLNVHPVPGGRGVSAGWGWAIPWNAPDKD
                     AAWQFISWVESAKIARQRALMGGAPTRRDILEDPELSRKFAAYYSTLKKVLPTAVPAI
                     PMMLEQIRFEDIVNDYLGAALLGEMSAEDALNRAAKELSRLR"
     gene            complement(2327..3160)
                     /locus_tag="PLM74_08920"
     CDS             complement(2327..3160)
                     /locus_tag="PLM74_08920"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HON43233.1"
                     /translation="MKRAMVEARFADVVSGVVLWAALLSVALPLVWILLTSLKPVSKI
                     YQMPPVLFFKPTLHNYYKALVENDSLRAAFLNSVIVAVTTSLVSVALAAPAAYAIVRF
                     VKRNYRARVAILAMQMLPPAVLAIPIALVARRLAMYDSLVSLIIACLTITLPFSIWLL
                     SAFLEGIPGSFEEAALIDGCSRFTAFLRVVVPVATPGLGAAAILSFIFGWNEFLYALV
                     LTGRTSQTLPVAACGFATSQGVQVEQLTVAVVLMALPAVLFGTVAQKYLVEGLTSNSL
                     K"
     gene            3378..4265
                     /locus_tag="PLM74_08925"
     CDS             3378..4265
                     /locus_tag="PLM74_08925"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HON43234.1"
                     /translation="MGSLVAPEQQDHAGLTLPLMRIRNAAASLGPAEERLAEFILDNP
                     EKVVYLSISELADACSVSDATIIRFCKRIGYRGYYEFKISLAVESSSPTKHFHENVAR
                     DDSTADIIRKVTADSVRALQDFESAIHPGQFERAVQCMAAAGKGRIEIYGVGGSAFIA
                     DMAYHKWLKLGLSVIAITDPHIQLMSASLLTRRDVALGISDSGTSVQIVYALEKARQS
                     GAATIAITGHDRSPICKVSDIVLLTRATDTVETESLCRRAVQVNLIECLAVAVGIKNY
                     EQSVETIRKTDAAVVSYKL"
BASE COUNT         1026 a         1382 c         1267 g          941 t
ORIGIN      
        1 ctccgtgcgg ttgcttgggc cgccgcttgt gagcacgtag actatgtcga aggtcttcag
       61 cgcatccagg gcccttatga ggactgcccc cagagccacc ggtctgatca gcggcatggt
      121 cacgtacctg aacacctgcc acctggtggc gccgtccacg tgagcggcct ccacgggttc
      181 ttgaggcagc gacaccagcc ctgcgtagag catgagcgcg accaaaggag tccattccca
      241 cacatccacc aggcacaccg acagcagcgc cgttcctccc tcgccaagcc aggcgcgggt
      301 aggcgtcccg agcagcccga gcagatagtt gaacacaccc agcgttgggt ggaggatgta
      361 cgcccacacc aggcctgtgg agatcggcgt gataatcgtg ggcatgaggt acaccgagaa
      421 gaatgcccgc gaataccgct gcctggtccg gagcgcaagg gctacaatca gcccaaaaac
      481 cgtttcaagc cccactgtcg ccacggtgaa taccgtagtc acccaaagcg aattcctgaa
      541 atcgcgctgc cacagcgcat cgatgtagtt gcgaatccct acgaaccggc tctgagtggg
      601 cgcgatgaaa gagtagtctg tgaagctcag gtatattacg tacagaagcg gaatcaacac
      661 tactatcccg agtatggcga gagaggggac caagaatccc agcgcagtat aggaaggcct
      721 cttcctctga tggactggtg ttgcggtcat catagatccc ctctctggtc actctctcag
      781 gctagtctct ctagtcagtc tctccgatta gtctctctga tcagtgcgcc cctagcgcag
      841 acgcgagagc tcctttgccg ctctgttcag cgcgtcttcc gccgacatct cgccaagaag
      901 ggctgcaccc aggtagtcgt tgacgatgtc ttcgaacctg atctgctcca acatcatcgg
      961 gatggccgga actgccgtag gcaggacctt cttgagagtg ctgtagtagg cggcgaactt
     1021 ccggctaagt tccggatctt ccagaatgtc gcgcctggtg ggcgctccgc ccatgagtgc
     1081 acgctggcgc gctatcttgg ccgactcgac ccacgagatg aactgccatg cagcgtcctt
     1141 gtcgggggcg ttccaaggaa tcgcccaacc ccagccggcc gatactccgc gtccgccggg
     1201 cacggggtgc acatttagct tgccggccac cttggacttg gcaggatcgt tcagcgggcc
     1261 cagcatccag ttgaacgtga ccatcgtggc gccctggccc tgcgccatcg cattgagagc
     1321 gtcgtcaaaa gagaacccca tggagcctgc cggcgccgcg ttcttcagag tgtccatgta
     1381 aaggttgagt gccttgactc ctgcctggct gttgatggtg accttcttgt tggtgtaaaa
     1441 gtcaccgccc aggccgaaga agtagttggc ccagtcacag taagtgtcgg actttttggg
     1501 tgtcatgatc gagccccaga tatcgacctt gccgtcgcca ttggtgtcgc gggtgaggaa
     1561 cttggcaacc tgcacatact cctcaactga gaggttatcg agatcgagtt ccttgccgaa
     1621 cttggccttg tactccttgc ggatcttggg gtcgtccaca atgtcggacc tggtgagcat
     1681 caccatgttg tactggtaga acggcagcat gtaagtcacg ccgtcgaaca cgcccgccaa
     1741 gtccagcatg gacgggtaat acacgtccat gttcaccgac ggcgtttcct taatgtagtc
     1801 atcgagaggg atgatccaac ggccattcac aaactggccg gtccagaacg gggcgtcgac
     1861 aactatcacg tcgtactgcc cgcggcccga cagcacgttg gtcatgagct tgtccctcat
     1921 aaccatgaac gtagtctttt ccacattgac cttaatacca gtggcctttt cgaactcagg
     1981 gatcagcttg ataacatatt ccgaatccgg aacggtctcg agcaggatgg taatggactt
     2041 ttccttagca gcctgcacac ccgtcacaag cgatagcgcc atggcaaaga ccagcgccat
     2101 agtaagcgcg cgggcaagga tggcccttga cttcatcttc ataccctcct agatcctaaa
     2161 tgctagatgt acctgatcac tgttatcaca atttgcttct tacggttatg ctgcttctac
     2221 tgctcctact gcttctactg cgacactact actggctctg ctgctggttt tgatgctagt
     2281 tctgccgttg gcacctccct caccaaacct tccgcaaccc tgtaccctac ttgagcgaat
     2341 tggacgtaag cccctcgacc aggtacttct gggctaccgt accgaagagg acagccggca
     2401 gagccatcaa cacaaccgcc accgtgagct gctccacctg caccccctgg gaagtcgcga
     2461 acccacacgc cgccacaggc agcgtctggc tagtccttcc cgtcaggacc agcgcgtaca
     2521 agaactcgtt ccaaccgaat atgaagctga ggatcgccgc ggctcccagc ccaggagtag
     2581 ccaccggcac gaccacacgc agaaacgcgg taaaccggct gcacccgtcg atgagcgcag
     2641 cctcttcaaa gctgcccggt atgccttcga gaaaagccga cagaagccag atggagaatg
     2701 gcagcgtgat ggtgagacag gcgataatca gcgacacgag gctgtcgtac atggccaacc
     2761 gccgggcgac taatgctatc ggaatggcca ggaccgccgg cggcaacatc tgcatcgcca
     2821 gtatggccac gcgcgccctg taattgcgct tcacaaagcg cacaatggcg taggccgcag
     2881 gcgccgccag agccaccgaa acaagggagg tggtcacggc cacaattacg ctgttgagaa
     2941 aagccgcccg cagagaatca ttctccacca gggccttata gtagttgtgt agcgtgggtt
     3001 tgaagaaaag aaccgggggc atttggtata tcttggacac cggcttaagc gacgtgagca
     3061 ggatccacac aagcggcaac gccaccgaaa gaagcgctgc ccagagcaca actccgctca
     3121 ccacatctgc aaatcttgct tcgaccatag cacgtttcat tgcgagtccc ccggttcaac
     3181 ccattgcttt gttgacatgt aaccttcatc ccgacacgaa gtattgcttc atcgagagag
     3241 ggtattctac ctcggtgcca cttttcctcc ttgttgtgac gaaattttga tatcaccgtt
     3301 ttgcaggacg taggcaatcg gcggcgaatc agatcattgg ctaagtcgca tttagttcaa
     3361 gacaggggtg cagtgccatg ggtagtcttg tcgctcccga acagcaggac catgcaggcc
     3421 ttacactacc gttaatgagg atcaggaacg cagccgcttc gctggggcct gctgaggaga
     3481 ggttggccga gttcatcctg gacaaccctg agaaggtcgt gtacctctcc atatcggagt
     3541 tggccgacgc ttgctcggtg agtgacgcga cgatcataag gttctgcaaa cggatcggat
     3601 accgcgggta ctacgagttc aagatcagtc tggcagttga gtcgtcatcg cccaccaagc
     3661 acttccatga aaacgtggcc cgagacgaca gcacagccga catcatacgc aaagtcaccg
     3721 ccgattctgt gcgggccctt caggacttcg agtcggccat ccatcccggt cagttcgagc
     3781 gcgcagtgca gtgcatggcg gcggcaggaa aaggaaggat cgagatatac ggagtgggcg
     3841 gatcagcctt cattgcggac atggcgtatc ataaatggct caaactcggg ctttcggtta
     3901 tcgcgataac cgatccacat attcagctca tgtcggcgag cctattaacc aggcgcgatg
     3961 tggctctggg catatccgat tcaggcacct cggttcagat tgtgtacgcc ctggagaaag
     4021 ccaggcagtc gggcgccgcc accatagcca taaccggcca tgaccgatcg cccatatgca
     4081 aagtgtccga catagtgctg ctgacacgcg ccaccgacac agtcgaaaca gaaagcctct
     4141 gccgacgagc cgtgcaggta aacctgatag aatgcctagc cgtggcagta ggcatcaaaa
     4201 actacgaaca gtcagtcgag acaatcagaa agacagacgc ggcagtggtg agctataagc
     4261 tgtagtcccg tagtcgaatc ctgtcttctg ttccattcca cccgggcccg cgatcgccac
     4321 gactaggcag cctgcttgtt atccatccac agatcggcgc ctgtgcccta cgggacacct
     4381 ttttcggcca tcctgcgtcg gtttacacag gaaccctgga aatgctgtcc cgtaggttac
     4441 aggttcgcgg aaacccccga cggccgttgc accgggcgaa atcccgggct acgataatcc
     4501 cagctagaag gcgtaggcgg cactggtggg catcgacacc ggggcgcgcg agatcgtcgg
     4561 ggatcggcgc ctgtgcccta cgggacacct ttttcggcca tcctgcgtcg gtttac
//
LOCUS       DAOTAC010000210         4604 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_15406_length_4604_cov_4.452627, whole genome shotgun sequence.
ACCESSION   DAOTAC010000210 DAOTAC010000000
VERSION     DAOTAC010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4604
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15406_length_4604_cov_4.452627"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            185..622
                     /gene="rplM"
                     /locus_tag="PLM74_08930"
     CDS             185..622
                     /gene="rplM"
                     /locus_tag="PLM74_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HON43235.1"
                     /translation="MSATYTPKPDDIVRKWYVVDASGKPLGRLATEVARVLKGKHKPM
                     YTPHIDVGDHVIVINADKVVLTGNKLHTKIHYWHTGYPGGIRQMTYEKFLATRPVRVV
                     EKAVKGMLPHTRLGRKMGMKLKVYAGPEHPHAAQKPEPLEITV"
     gene            649..1041
                     /gene="rpsI"
                     /locus_tag="PLM74_08935"
     CDS             649..1041
                     /gene="rpsI"
                     /locus_tag="PLM74_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011876644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HON43236.1"
                     /translation="MAQLQFSATGRRKCSVARVRLVPGTGRILVNEKPANEYFGRRTL
                     ELVVNGPFEVTQTGGKYDAIADVIGGGVSGQAGAVRHGIARALVEVDPELRSALKKAG
                     YLTRDPRMKERRKYGLKKARKAPQFSKR"
     gene            complement(1163..4081)
                     /locus_tag="PLM74_08940"
     CDS             complement(1163..4081)
                     /locus_tag="PLM74_08940"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HON43237.1"
                     /translation="MRLRYTKTPRHAAGAGALTRAVIVAIAVASIIALPAAVPVKSAA
                     AGIADELLAKSLSIPLAPGVSFERRSAVTGAGWVGLSIIRVDLTSPYVTVGPALAGGV
                     LTSPAKATEITEAAGLVAAINGDFFDAGVTGAPLSLLIADGQLVRSPRNDPDFASMAI
                     AGETGILGSWSWSGQLIGPGGLKIPISAVNEVSVPANGAVLYTGHWSWLRNPPTNGVT
                     CIAIRDGVVQSRIAGWPRPAPSSTSEELGLIYLAARGSAATSAASLSVGDAVEISGQL
                     RPDAENIIAAFSGKPVLLRDGVIASDLWKHTGIQSLFAAPRSAAGLTQDSRTLLLVVA
                     DGRQSMARGLTLSELAELMRSLGAWNALNLDGGGSSSAVVRDPFSGEIVLANRPSGAS
                     QRPVPYVVGVQALPPAAGNEAEPDIAFLLISADELQSRAHLGDRPSLAAAVQGGPAHL
                     VEQDLQVEAGRQLLLSARLYDSLMRMVDPDTLHTVEVRWNVIPPKSLRGRDPASLESG
                     VWAVSTDTQSATLCPQTPGRFEIEATAVWTRPHSSGELIVERSNTVSFGVRVNQPAPA
                     PGSDASPSVEAAFGPILIEGFDNVDAPGWAGWRSSSSSPSVKHSASFAAPPDTELDVT
                     LPGLPSPAPAGLAAMLSYDFNSPDATRAVYLKPSEPISLPEGTRIIGMWVWGDGGGHW
                     LRATVADEAGNRFPIDFPRMHWTGWRYVQAGLPSELAGPAWLAQVYLVEFKPEMQGAG
                     TVYIDSIAAMGASAPATGDGSSPTAPAASPSSSPEPSSETHLPTPPLAPPSTSSVSSS
                     VSQSASSTSPAPPSATPAPARTRTDAPDAPKPLEPGEDLTSLVRPVPGRHVITIDGSR
                     PSLPWPWLVQEFDTFRASDDMELVVVLNGATAVDIKSLVPGGTFKDSLQAELLLDLLN
                     TMADGSTSVVLVQDPRPSPLPAHSSGAKMEPDGLYMPGASPLAAPLWTMLGQVKVIWK
                     "
     gene            complement(4167..>4604)
                     /locus_tag="PLM74_08945"
     CDS             complement(4167..>4604)
                     /locus_tag="PLM74_08945"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HON43238.1"
                     /translation="GGGCELALACDIRIASENARFAQPETGLGIIPGFGGSVRLPATI
                     GRARAAYLILTGRQVNAADALSMGLVSEVMPRESLMARAREIAAELATRSPVALAHAK
                     RAMATRIKAYEREALEFGRCFETDDPKEGMSAFLERRKPGYAR"
BASE COUNT          895 a         1378 c         1472 g          859 t
ORIGIN      
        1 catcaattcc atcttggctc ggagaaaagc ccctacaact acttgactca gtccgcgacg
       61 cggggtgcct tgacagtgca tgttcactat attagaattg cttcgtgagt cttgtgccag
      121 tggagccaag ccccggaacc actgacgaca ctccgacgag cagttcaatc tgggagggaa
      181 ctcgatgtcg gccacatata cgccgaagcc cgatgacatc gtccgcaagt ggtatgtcgt
      241 ggatgcctcc gggaagcccc tgggcaggct cgcgactgag gtggcccgtg tcctgaaggg
      301 caagcataag ccaatgtaca ctcctcacat cgatgtgggc gaccatgtca tagtgatcaa
      361 tgctgacaag gtagtcctca ccggcaacaa gctacacacc aagatacact actggcacac
      421 cggttacccg ggcgggattc gccagatgac ctatgagaag ttccttgcga ccaggcccgt
      481 gcgtgttgtg gagaaggcag tcaagggtat gctgccgcac acacggctgg ggcgcaagat
      541 gggaatgaag cttaaggtat acgcgggccc cgagcacccg catgcggcgc agaaacctga
      601 gccgctcgaa ataacggttt gatccgggcg aggaaggggg ataattctgt ggcgcagctg
      661 cagttttccg cgacaggcag acgcaagtgc tcagtcgctc gcgtaaggct agtgccggga
      721 accggcagga tcctggtcaa tgagaagcct gcaaatgagt atttcggccg ccggactctc
      781 gagctggtgg tgaacggccc gttcgaagtt acgcagacgg gcggaaaata cgacgccatc
      841 gccgacgtaa tcggaggcgg ggtgagcggg caggcgggcg ctgtgcggca cggcattgcc
      901 agagcgcttg ttgaggtcga tcctgagctt aggtcggcac tcaagaaggc cggctacctc
      961 acgcgcgacc cgcggatgaa agagcgtcgc aagtacggcc tgaagaaggc tcgtaaggcg
     1021 cctcagttct ccaagagata agctgagggc actccacgat gctggtaaga tgaaggacgg
     1081 gctctgtgcc cgtcctttcc tctacgagag cttagtggcg atgcctacgc tatcggcggt
     1141 gagactcaag cagcgcatca gattacttcc agatgacttt gacctgcccc agcattgtcc
     1201 agagcggcgc agctagtggg cttgcgccgg gcatataaag cccgtctggt tccatctttg
     1261 ctcccgagct gtgcgcaggc agcgggctgg gccggggatc ctgcaccaag accaccgatg
     1321 tgctcccgtc tgccatcgta ttcagcaaat ccagaagcag ttctgcctga agactgtctt
     1381 tgaatgtacc gcctgggacg agtgacttaa tatcgactgc tgtcgcgccg tttagcacca
     1441 cgaccaactc catgtcgtcg ctggccctga acgtatcgaa ctcctggaca agccagggcc
     1501 acggcaggga agggcgggat ccgtcgatgg ttatgacatg ccgcccggga acggggcgaa
     1561 ctaggctcgt caagtcttcg cctggctcaa gcggcttggg cgcatcaggc gcatcggttc
     1621 gggtacgggc tggggcaggt gtagccgaag gtggcgctgg cgacgtcgag gacgcgctct
     1681 gtgacacgga agatgacacg gaagatgtgg acggcggcgc tagcggcggt gtaggtaaat
     1741 gcgtctcaga cgaaggttca ggtgacgatg agggcgaggc cgcaggtgca gtcggagatg
     1801 acccatcgcc ggtcgccggc gcgctcgcgc ccattgcggc gatgctgtcg atgtacacgg
     1861 ttccggctcc ctgcatttcg ggcttgaact caaccaggta cacttgcgca agccacgccg
     1921 gccctgccag ctccgaaggc aagcccgcct gtacgtaacg ccagcctgtc cagtgcatcc
     1981 gcgggaagtc tatggggaac cggtttcccg cctcgtccgc taccgtggcc cggagccaat
     2041 gcccgcctcc atccccccac acccacatgc ctatgatacg tgtgccctcc ggcagcgaaa
     2101 tcggttcaga cggcttgaga tagactgcac gtgtcgcatc cgggctgttg aagtcgtagc
     2161 tgagcatcgc agccagccct gcgggcgcgg gcgacggcag gcctggcaac gtaacgtcga
     2221 gttctgtatc gggcggcgcg gcgaaggatg ccgaatgctt cacgctcgga gatgaagatg
     2281 acgatcgcca gccggcccag cccggtgcgt ccacgttgtc aaaaccttca atcaggatgg
     2341 gcccgaatgc ggcctccacg ctcggcgaag cgtcggatcc gggcgcgggc gcgggctgat
     2401 tgactcgcac tccaaatgag actgtgttac tgcgttccac gatcaactcg cctgatgaat
     2461 gcggcctggt ccagactgca gtggcctcga tctcgaaccg gccaggcgtc tgtggacaca
     2521 aggtggcgct ctgggtatcg gtcgagactg cccagacccc ggactcgagg cttgcagggt
     2581 cacgtccacg cagggacttt ggggggatca cattccacct gacttcgact gtgtggagtg
     2641 tatctggatc gaccatgcgc atcaggctgt cgtagagccg ggccgacagt aggagttggc
     2701 ggcctgcttc gacctggaga tcctgctcta cgaggtgcgc cggtccgccc tggacggcgg
     2761 ctgcgaggga cgggcggtcg cccaaatggg ctcggctttg gagttcatcc gccgatatca
     2821 agaggaatgc gatgtcaggc tcggcctcat tgcctgcggc cggcggaagt gcctgcacac
     2881 ctactacata cggcaccggt ctttgcgatg cgcctgacgg ccggttggcc agtactatct
     2941 cgccggagaa ggggtcgcgt acgacggctg acgatgaccc tccgccgtcc agattcagcg
     3001 cgttccaagc gccgagcgaa cgcatgagct ctgcaagctc gctcagggtc agcccgcgtg
     3061 ccattgattg ccggccgtct gctaccacga gcagcaaggt tcggctgtcc tgtgtgagcc
     3121 cggctgcgct gcgcggcgcc gcgaagagcg actggatgcc tgtatgcttc cataagtcgg
     3181 acgcgataac gccgtcgcgg agcagcactg gtttgcctga gaatgccgca atgatgttct
     3241 cggcgtcagg gcgcaactgt cccgagatct ccaccgcgtc tcccacagag agggaggcgg
     3301 cggaagtggc tgcgctgcct cttgcggcca gatagatgag gcccagctcc tcagatgttg
     3361 aggacggcgc ggggcgcggc cacccggcga tccgagactg gacaactccg tcgcgaatgg
     3421 cgatgcacgt taccccgttg gtgggcgggt tgcgcagcca tgaccagtgg ccggtgtaca
     3481 ataccgcccc gtttgccggc actgagactt cgttcaccgc tgaaatcggt atcttgagcc
     3541 cgcccgggcc aatcagctga ccagaccagc tccagctgcc caggatgccc gtttcgcctg
     3601 cgatggccat cgacgcgaag tcgggatcgt tgcgcggtga gcgcaccaac tggccgtctg
     3661 cgatgagcag cgaaagcggt gctccggtga cgccggcatc gaagaagtcg ccgttgattg
     3721 cggccaccag accggctgct tcggtgatct ccgtggcctt ggcgggcgag gtcagcacac
     3781 caccggcaag ggccggtcct accgtaacgt agggggaggt caggtcgact cggatgatcg
     3841 acaggccgac ccatcccgcg ccagttacgg cgctgcgtcg ctcgaatgaa acacccggcg
     3901 cgagtggaat cgacagcgat ttggcaagca gctcatcggc aatgcccgcc gctgcggact
     3961 tgacagggac tgcggcgggc agggcaatta ttgaggcgac ggcgatagcg acgatgacgg
     4021 cacgtgtcag tgcccctgcc ccagctgcat gtcttggtgt tttggtatat cgtaatcgca
     4081 tcatatcaca tcacagtagt tgcacagagt ctcgcagact ctcgcagaat cgtgcagaat
     4141 catgcaggat cacggccgat cgccgatcag cgtgcgtagc cgggcttgcg gcgttccaga
     4201 aaggctgaca tcccttcttt cggatcatcg gtctcgaaac acctgccgaa ctcaagcgct
     4261 tcgcgctcat acgccttgat gcgtgtggcc atcgctcgtt tggcatgagc tagggccaca
     4321 gggctgcgcg tcgccagttc ggcagcgatc tcgcgagctc gagccatcaa ggattcgcgc
     4381 ggcatcactt ctgatactaa tcccatggat agcgcatctg ccgcgttcac ctgcctgccg
     4441 gttaggatca ggtaggccgc acgggctcgg cctattgtag caggaaggcg cacactgcct
     4501 ccaaagccgg ggattatgcc cagacccgtc tctggttggg cgaatcgggc gttttctgag
     4561 gcgatcctta tgtcgcacgc cagggcaagt tcacatccgc cgcc
//
LOCUS       DAOTAC010000211         4589 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_15476_length_4589_cov_4.927658, whole genome shotgun sequence.
ACCESSION   DAOTAC010000211 DAOTAC010000000
VERSION     DAOTAC010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4589
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15476_length_4589_cov_4.927658"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /locus_tag="PLM74_08950"
     CDS             complement(<1..903)
                     /locus_tag="PLM74_08950"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HON43239.1"
                     /translation="MLEQRRSATARCTGIATIYEIAKKAGVSTATVSRALSGGGYVRK
                     ELREHILQVAKAMDYQPNSFARGLVTRQSYILGLIVPDICNPFFPAVARGVEDVANEN
                     GYNVLLCNTDGSPKKENNYINVLRSRQADGVIFTTSQVSSKNVRTLMNSGIPVVLADR
                     RMDLECDTVAADNVEGAFRATQHLLDLGHARIGIITGPMGVATSEDRVEGYKGALSER
                     GIAPNPDLVREGDYRERSGYDGAMELIRLSDPPTAIFACNDLMAVGALHALEESQLRV
                     PDDVSVVGYDDITVASVARPRLTTV"
     gene            complement(1241..3208)
                     /gene="ftsH"
                     /locus_tag="PLM74_08955"
     CDS             complement(1241..3208)
                     /gene="ftsH"
                     /locus_tag="PLM74_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018035257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HON43240.1"
                     /translation="MKSLALWLLLGLIAVSISNSLFAGKPRVDDISASQFMKYWQDRK
                     VTQLEIVGENAIRGSLDDGRKFKLYYPDVKGLVEDVRNDPELASRVEVSMTPESQGAW
                     WIALIPNVISIVLLFAVFFFIMNQLQGGGNKAMQFGRSRAKLHAGERGRVTFEDVAGV
                     DEVKEELVEIVDYLKHPKRYIELGARIPRGILLVGPPGTGKTLLAKAVAGEADVPFFS
                     VSGSDFVEMFVGVGASRVRDMFDQAKRNAPCIVFIDEIDAVGRQRGAGLGGGHDEREQ
                     TLNQLLFEMDGFDPNSGIIILAATNRPDVLDPALLRAGRFDRHIVVDAPDQRGREEIL
                     KIHAKNKPLETDIDLKALSKRTPGFTGADLANLLNEAALLAARGNKKRVSMAEMSEAI
                     ERIVAGPAKKGRIMSEREKDLVAYHESGHAVLAHLLPGADPVVKVSIVGRGRAGGYTL
                     MIPEDDRRYRYATKSEIMNEIIVGLGGRVAEELVLGEISTGAGNDLERVSKTARKMVT
                     EYGMSDLGPMTFGRSQEEQVFLGRDISRDRNYSEEVAAKIDEEVHKIVTAAHDTARRI
                     LSENIDSLHKLAQTLKEQETVEAEELEALLAGPAGESCDQAKAAQSAARSAGSEAQTP
                     SAGDAKGALAAIEERLKSGGKREPSPEGAPA"
     gene            complement(3422..>4589)
                     /gene="tilS"
                     /locus_tag="PLM74_08960"
     CDS             complement(3422..>4589)
                     /gene="tilS"
                     /locus_tag="PLM74_08960"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF023162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HON43241.1"
                     /translation="GLAGIPPIRKLDEGISVIRPFIEVSREQIEQYCEKRRLATRTDP
                     TNVDTQYFRNKVRCELLPLLEREYNSNIRAGLARLARLASSDEQYLEEQAVRLLDAAR
                     AAVLGSAGAADLRRREPQVELDREMMRQAAEPVASRAFALAFAEAAGDRRDLYWPNID
                     DMAALARTGNVGNSIDLPGSLRARLTYDTIIIERVAEPAREGADEPGALAESTAEQID
                     VPVRVPGAMPLAWAQVTVYAACGERSALERAPGAHADPDADTSIEELACMANRALADS
                     GFRGLRVDASGIGRKWAVFDADRVDPDALVVRRWRAGDRIRPLGMAGAKKLSDLFVDE
                     KVPRPVRSRLAVLAMGDEALWVVGVRASGRGTVTSSTRRLAVFAADTDDCVDDA"
BASE COUNT          836 a         1492 c         1253 g         1008 t
ORIGIN      
        1 gacagtggta aggcggggcc gggccacaga cgccactgtg atgtcgtcgt acccgaccac
       61 cgaaacgtcg tccggaactc gcagttgcga ctcctccaac gcatggagcg cgcccactgc
      121 catcaggtca ttgcacgcga atatggctgt gggcgggtca gatagcctga tgagctccat
      181 ggccccatca tagccactcc tctcgcggta gtcgccctcg cgaaccagat cagggttcgg
      241 ggcgataccc cgctcgctca gagcgccctt gtagccttct actctgtctt cgctggtcgc
      301 aactcccatt ggcccggtta tgatgccgat tctggcatgc ccaagatcca gtagatgttg
      361 ggtagcacgg aatgcccctt ccacgttgtc ggctgcaact gtgtcgcact ccaagtccat
      421 gcgcctatcc gccagtacca caggtattcc cgagttcatc agcgttctga cgttcttgga
      481 gctaacctgc gatgtagtga atattactcc atccgcctgt cggctgcgca agacgtttat
      541 gtaattgttc tccttcttgg gactcccatc agtgttgcac aggagcacgt tgtacccgtt
      601 ctcgttggct acgtcctcga ctcccctggc gacagcgggg aagaacgggt tacatatatc
      661 ggggactatc agaccaagta tgtagctttg gcgggtgacc agtccacgcg caaacgagtt
      721 tggctgatag tccattgcct tcgctacctg caggatatgt tccctaagct ctttgcgcac
      781 atacccgccg ccggagagag ccctggagac agtagcagtg gaaacgccgg ctttcttggc
      841 aatctcgtat atggtagcta ttcctgtgca cctcgcagtc gcgctccgcc tctgctccag
      901 catgtcttga cgacgccttg ccgcgctcgg aggctgcatg caagcgctta catatgatag
      961 cggttacaca tattctccgt gatctcgatc gttccttctt gtttggaccg ggtttttcgt
     1021 tacatgaccc gcgaaatgcg actcgtgcgc tgtgtagggc gttttgacgg agtggttact
     1081 gcgaagccgg cgaagaggtc cgcgtgccgg cgcggaagag gcggtgtccg ctcagaggcg
     1141 gcagtccctg caggaagcta atatgagggc tccgtgaagc gtgtgaacac gtctccagga
     1201 gccctcatca ctcgtcatat gcggcagttt caggcgaggc tcaggccggc gccccctccg
     1261 ggctgggttc gcgctttccg cccgacttca gcctctcttc gatggcagcc agggcgcctt
     1321 tggcgtctcc ggcagatggt gtttgggcct ccgatcccgc cgaccgagcg gccgattgcg
     1381 cggccttcgc ttggtcgcag ctctcgccgg ctgggcccgc aagcagggcc tcgagttcct
     1441 ctgcctcgac tgtctcctgc tctttcaggg tctgggccaa cttgtgcagg ctatcgatat
     1501 tctcgctcag tattctcctc gcagtgtcat gagcagccgt gactatcttg tggacctctt
     1561 cgtcgatctt cgcggccact tcttcgctgt agttgcggtc gcgggagatg tcgcgcccca
     1621 ggaagacctg ctcctcctgg cttcggccga atgtcatcgg gccgaggtcg ctcatgccgt
     1681 attcggtgac catcttgcgg gccgtcttgc tcacgcgctc caggtcgttg ccggcgccag
     1741 tgctgatctc gcccagcacc agctcctcgg cgactcgacc gccaaggccc actatgatct
     1801 cgttcattat ctcagactta gtggcgtacc ggtatctcct gtcatcctcc ggtatcatca
     1861 gggtgtagcc tcccgctcgc ccccggccca ctatcgatac cttgacaacc gggtcggcgc
     1921 cgggcaggag gtgggccagc accgcatggc ctgactcatg gtaagccact agatccttct
     1981 cgcgctcgct catgatccgg cccttcttgg caggccccgc cactattcgc tctatcgcct
     2041 ccgacatctc agccatggat actctcttct tattccccct ggccgccagg agtgccgctt
     2101 cgttcaacag gttcgcaaga tcggcgccgg taaagccggg agtgcgcttc gacagcgctt
     2161 tgaggtcgat atctgtctca agaggcttgt tcttggcatg gatcttgagt atctcttccc
     2221 tgccgcgctg gtcgggggca tccaccacta tgtggcggtc gaatctgcct gcacggagca
     2281 gcgcagggtc aagaacatcg ggcctgttcg tcgcggctaa gatgatgatc ccggaattag
     2341 gatcgaatcc gtccatctca aagagcagct ggttaagcgt ctgctcacgc tcatcgtgcc
     2401 cgccgcccag ccccgcgcca cgttggcggc ccacagcgtc gatttcgtct atgaacacta
     2461 tgcacggagc gttccgcttc gcctggtcga acatgtcgcg cactcggctt gcgcccacac
     2521 ccacgaacat ctccacaaag tcagaaccgc tgacactgaa gaaaggcaca tccgcctcgc
     2581 ccgcaacagc cttggcaagc agggtcttgc cggttccggg aggtcccacc agtagaatcc
     2641 ccctgggtat cctggctccg agttcgatgt atcgcttggg gtgtttcagg tagtccacga
     2701 tttcgacgag ttcctctttg acctcgtcca ctccggctac gtcttcgaac gtcacgcggc
     2761 cacgttcacc ggcatgaagc ttggcccggc tccgcccgaa ctgcatcgcc ttgttgcccc
     2821 cgccctgaag ctggttcatg atgaagaaga acacagcaaa gaggaggaca atcgatatta
     2881 cgttcggtat gagcgctatc caccaggccc cctgggactc aggagtcatg ctgacttcta
     2941 cacgcgaagc aagctccggg tcgtttcgaa catcttcgac caggcccttc acatccgggt
     3001 agtacagctt gaactttcgc ccatcgtcca aagagccccg gattgcgttt tcgccgacta
     3061 tctcgagttg ggtgactttc cggtcttgcc agtatttcat gaactggctg gccgagatat
     3121 cgtcgactcg gggcttgccc gcaaagagcg agttagatat ggagactgct atcaggccga
     3181 gcaacagcca tagagcaaga cttttcataa gcctattgtt caccgtaagt aggcgcaaga
     3241 agccccgtgt accagggcat cgcggtatat gcgccttcct ccttccccga ctaattcagg
     3301 tgcatgctgc aaatgcaatc ccaaatacta gataattctc gttgtgcgtg aactcattat
     3361 agcatagcgc cttttctatc acaacgaaac gcacagctgt atggtttcca tctcaaccgg
     3421 cttatgcatc gtcaacgcaa tcatccgtat cggccgcgaa gacagccaga cgccttgtcg
     3481 aagacgtcac cgtccctctt ccgctcgccc ttacgcccac gacccaaagt gcctcatcgc
     3541 ccatggccaa gacggccagg cgcgaccgaa cgggccgagg taccttctca tctacaaaca
     3601 ggtcggacag cttcttggcc ccggccatcc caagcgggcg gattctgtcg cctgcacgcc
     3661 accggcggac gaccagggca tcggggtcca cgcggtcagc gtcgaacacc gcccacttcc
     3721 gaccgattcc ggaggcatcc acgcgcagcc cgcgaaatcc cgaatcagcc agggcgcgat
     3781 tggccatgca ggccaactcc tcaatgctcg tatccgcatc cggatccgcg tgtgcccccg
     3841 gcgccctctc cagggccgac ctctcgccac aagccgcata gactgtgact tgagcccacg
     3901 cgagaggcat agcccccggc acgcgcaccg gaacatctat ctgttccgcg gtcgattcgg
     3961 cgagcgcacc gggttcatct gcaccttccc gtgcaggttc ggctacacgc tctattatta
     4021 tcgtatcgta cgtcaggcgc gcccgtagac ttccgggcaa gtctatcgaa ttgcctacgt
     4081 tcccagtccg tgccagagcc gccatgtcgt ctatgttagg ccagtagaga tcgcgcctgt
     4141 ctccagctgc ttcagcgaag gccaacgcaa atgcgcgtga agcaaccggc tctgcggcct
     4201 gtcgcatcat ctcgcggtct agctccacct gcggctcccg ccggcgcagg tccgcagcgc
     4261 cggcgctgcc taaaaccgct gctctagccg catcgagaag gcggactgcc tgctcttcta
     4321 ggtactgctc gtccgatgac gcaaggcgcg caagccgggc cagtccggcg cggatattcg
     4381 agttgtactc gcgctccaga aggggtagga gttcgcaccg caccttgttg cggaagtatt
     4441 gcgtatcgac attcgtcggg tcggtacggg tcgcaagacg ccgcttctcg caatactgct
     4501 cgatctgctc ccgcgacacc tcgataaacg gcctgatcac cgagatcccc tcgtctaact
     4561 tgcgaatggg cgggatgccg gccagcccg
//
LOCUS       DAOTAC010000212         4585 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_15492_length_4585_cov_4.293598, whole genome shotgun sequence.
ACCESSION   DAOTAC010000212 DAOTAC010000000
VERSION     DAOTAC010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4585
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15492_length_4585_cov_4.293598"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..820)
                     /gene="yunB"
                     /locus_tag="PLM74_08965"
     CDS             complement(113..820)
                     /gene="yunB"
                     /locus_tag="PLM74_08965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HON43242.1"
                     /translation="MRSHQLGSGQVRRGRGSRRRQGLLVAVLVCLGVGFLGFTFVEVR
                     LKPTLLQLAEARARAVATTAVNTALSETSALEIKYEDLIDWKTDSQGNIVAVQPNTGE
                     INRIAASTTTRVQAALRDIKNVRISVPIGQVFGSAILANLGPWLTVSVVPIGVVSTSV
                     TDQFETAGINQLRHRVYLEIEAYVKILVPLVSSNMTVKTSMPIAESIILGDVPQFYVH
                     MGEPGSLVKGLLSTDKP"
     gene            complement(988..1911)
                     /gene="fba"
                     /locus_tag="PLM74_08970"
     CDS             complement(988..1911)
                     /gene="fba"
                     /locus_tag="PLM74_08970"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012035907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HON43243.1"
                     /translation="MGLVSSKSILDAANKGRYAVGAFNVNNLEFAKAVIGAAAEERSP
                     IIVQVSESAIAYAGMKQITGIVAGLAGQSDVPVALHLDHGRNWATIVQCITNGFTSVM
                     IDGSHLPFDENVAITRKVVEVAHAAGVSVEAELGKLAGIEDYIVVDERDAFLTDPDEA
                     LAFVERTGVDALAVAIGTSHGPRKFKGEPVLAIDLVSKIKEKVGIPLVLHGASGVPAE
                     IMEVGERFGAEWGGSKGIPDGSIVDAIARGINKVNIDTDMRLAFIASVRETLWTRPEV
                     TDPRDLLKPAMAKVKSVAAGKMHLFGSAGRA"
     gene            complement(1935..3155)
                     /gene="tyrS"
                     /locus_tag="PLM74_08975"
     CDS             complement(1935..3155)
                     /gene="tyrS"
                     /locus_tag="PLM74_08975"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HON43244.1"
                     /translation="MDPKEQLEVIKRGAAEVISEEDLLAKFGKSMRTGRPLRVKLGLD
                     PSAPDIHIGHTVVLHKMRQFQDMGHEGIIVIGDFTGRIGDPTGRREARKQLSEEEVRA
                     NAATYCEQVFKIIDAAHTRVVFNSEWLGKLMFADIIELASKVTVARMLERDDFETRYR
                     EGRPIGIHEFFYPLMQAYDSIALEADVELGGTDQKFNNLMGRTMQREFGQESQCVVLM
                     PILPGLDGVHKMSKSLGNYIGISEEPSEIYGKTMSIPDELIVPYVNSTTTFALAEKVA
                     IERDLESGALHPMKAKRKLARKLVAQYHGEDRARLAEDEFDRVFSKGQLPDDMPEVCL
                     AAHELADGTISVVALMVRSGLAPSNSEARRLVEQGGVRVDDRRVADPTQEIAPRDGMV
                     LRVGKRRFARVLVR"
     gene            complement(3253..3390)
                     /locus_tag="PLM74_08980"
     CDS             complement(3253..3390)
                     /locus_tag="PLM74_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43245.1"
                     /translation="MNEIAEGWRVSGIGLLSVFGVLLLFYIMLHVLMKLFPNKDENDS
                     H"
     gene            complement(3423..4505)
                     /locus_tag="PLM74_08985"
     CDS             complement(3423..4505)
                     /locus_tag="PLM74_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HON43246.1"
                     /translation="MFAIGGALIYLAVKKEYEPTLLLPIGFGAILTNIPGSSAIGEHG
                     FLTILYRAGIENELFPILIFIAVGAMIDFGPLLRRPFMLFFGAAAQFGIFATLMTGLY
                     LGRLHGVASFIPAFTLKQAASIGIIGAADGPTSIYVSGLFAPELLGPITVAAYSYMSL
                     VPLVQPPVIRLLTTPEERRIHMEYQEFRVSKTVRVLFPIMVTVIAGIIAPISVGLIGS
                     LMLGNLLREAGVVERLSKSAQNELANIVTLFLGIAIGSTMVADRFLQPSTLFIVLMGL
                     VAFIFDTAGGVIFAKTLNLFLKRKVNPMIGAAGISAFPMSARVIHKMGIEEDPTNYLL
                     MHAVGANVAGQVGSIIAGAMILALIH"
BASE COUNT          959 a         1443 c         1222 g          961 t
ORIGIN      
        1 tctaggctcc acgctgtttg gttttcaagg aacatccgcg gccttagcgc cgcgcgcaag
       61 tgttactata tcacctgccg gaagtttgcg caaggggtca ccccgacaat tttcacggtt
      121 tatccgtgga gagcaggccc ttgacaagcg aacccggttc gcccatatgt acgtagaatt
      181 gcgggacatc gccgaggata atcgactccg caatcggcat gctcgtcttt accgtcatgt
      241 tcgatgaaac tagcggcaca aggatcttta catatgcctc aatctccagg tagaccctgt
      301 gcctgagctg gtttatcccc gccgtctcga attgatccgt caccgacgtg ctcacgaccc
      361 ctatgggcac taccgatacc gtgagccacg ggccaaggtt ggcgagtatg gcgcttccga
      421 acacctgccc gataggcacc gatatcctga cgttctttat gtcgcgcagc gcggcctgaa
      481 ccctcgtcgt ggtgctcgcc gctattctgt ttatctcacc cgtattcggc tgcactgcca
      541 caatgttgcc ttggctgtct gtcttccaat cgatcaggtc ttcgtatttg atctccagcg
      601 ccgatgtctc cgaaagcgcc gtattcaccg cggtcgtcgc cacggctctg gcgcgggcct
      661 cggccagttg cagcaaggta ggtttgagcc gaacttccac gaacgtaaac cctaggaagc
      721 ccacgcccag gcagaccagc accgctacga gcaggccttg tctacgtcga ctcccacgcc
      781 cgcgtcgaac ctggccgctg ccgagctggt gtgaccgcac acctcgcagc agaccggggc
      841 ggcgcggccg aaacttgggt ctggcagatc cgccagatag gaaccgcagg agccaagcag
      901 gggcgggaaa ccccagcggc agcctgacac acaaaaaaac cacccctccc catcgtatgc
      961 aggaggggtg gtccctgttc acaagtgtta cgctcgccct gcgcttccga aaaggtgcat
     1021 cttgccggcg gccacggact tcaccttggc catggccggc ttaagtagat cgcgcgggtc
     1081 ggtcacctct ggcctcgtcc agagagtctc gcggacactg gcgatgaagg ccagacgcat
     1141 gtcggtatcg atgttgacct tgttgatgcc acgggcgata gcgtcaacga tgctgccgtc
     1201 agggatacct ttggatcctc cccactcggc cccgaaccgc tcgccgacct ccattatctc
     1261 ggcgggcacc ccggaggccc catgcagcac cagcggtatg ccaaccttct ccttgatctt
     1321 cgacacgaga tcgatagcca gcaccggctc acccttgaac ttcctcgggc cgtgggaagt
     1381 cccaatcgcc acagcgagcg cgtccacacc cgtccgctca acgaaagcca aggcctcgtc
     1441 gggatcggta aggaatgcgt ctcgctcatc cacaactata tagtcctcga tgcccgcgag
     1501 cttgcccagt tccgcctcaa cactcacgcc tgcggcatgc gcgacctcta caaccttccg
     1561 cgtgatcgcg acattctcgt caaaaggcag atgcgaaccg tctatcatga ccgaggtgaa
     1621 accgttcgtt atgcactgca cgatagttgc ccagttgcgc ccgtgatcca gatggagcgc
     1681 cacgggcacg tcggattggc cggcaaggcc ggcgacgatc cctgtaatct gcttcatccc
     1741 agcgtaagca atggcgctct ccgatacctg aactatgatg ggcgatctct cttccgcagc
     1801 agcgcctatg actgccttcg cgaattccag gttgttgacg ttaaacgccc cgaccgcata
     1861 gcgccctttg ttggccgcat caagaatcga tttcgatgaa accagaccca cgtttcgcca
     1921 cctccagtat tctcctaacg tactaggact ctagcgaacc gtcgcttgcc aactctgaga
     1981 accatgccgt cacgaggcgc gatctcctga gtggggtccg ccactcgccg gtcatccacc
     2041 cgcacgccgc cctgctccac aaggcgcctg gcttcgctgt tactcggcgc gagcccggat
     2101 ctgaccatga gggctacgac cgagatcgtc ccatctgcca gctcgtgcgc cgcgaggcac
     2161 acctcaggca tgtcatctgg cagctggcct ttgctgaata ccctgtcgaa ctcgtcctca
     2221 gccagcctag cccggtcttc gccgtggtac tgcgccacca acttccgagc gagtttcctc
     2281 ttggctttca taggatgcag agccccggac tccaaatcgc gctcaatggc gaccttctcg
     2341 gccagggcaa atgtcgtagt gctgttgaca tagggcacta tgagctcgtc aggtatagac
     2401 atagtcttgc cgtagatctc ggacggctcc tcgctaatgc ctatgtagtt gcccaggctt
     2461 ttactcatct tgtggactcc gtccagacca ggcagtatgg gcataagcac aacgcactga
     2521 ctctcctgcc cgaactcgcg ctgcatcgtg cggcccatca ggttgttgaa cttctggtcg
     2581 gtgcccccga gctcaacatc ggcttcaagg gcgatcgaat cgtatgcctg catgagcggg
     2641 tagaagaact cgtgtattcc gatgggccgg ccctctcgat atctggtttc gaaatcgtcg
     2701 cgctctagca tccgcgccac ggtgaccttc gatgccagtt cgatgatgtc ggcgaacatc
     2761 agcttgccca gccactccga gttgaagacg accctggtgt gcgcagcatc gatgatcttg
     2821 aaaacctgct cgcagtatgt tgccgcgttg gcgcgcacct cttcctccga gagctgcttt
     2881 cgcgcttctc ggcgccctgt aggatcgcca atcctgccgg tgaaatcgcc gatcacgatt
     2941 atcccttcgt gccccatatc ctgaaattgc ctcatcttgt gcagcacgac ggtgtgccct
     3001 atgtgaatgt caggcgcgga agggtccaac ccaagtttca ccctgagagg cctacctgtg
     3061 cgcatcgact tgccaaattt cgcaagcagg tcttcctcag agatgacttc ggcggcgccc
     3121 ctcttgatga cctctaactg ttccttggga tccattgcca gatgccccct atagcacgac
     3181 tgtgtgcgac gcagcagcgt gcagcgtcaa gcattatgta gagtctatca cagccgcgaa
     3241 accagggtca attcagtgcg aatcattctc gtccttgttg ggaaacagct tcatcagcac
     3301 atgcaacatg atgtagaaca gcaacaagac accgaaaact gatagaagcc caatgccgct
     3361 cacacgccag ccctcagcga tctcgttcac tcacaaccgg ctcctttctc tgtcacccgg
     3421 gatcaatgta tcagcgccag aatcatggcg ccggcgatta tggagccgac ttggcccgct
     3481 acgttggccc ccacagcatg catcaaaagg tagttagtgg gatcttcctc gatgcccatc
     3541 ttgtgtatca cccgtgccga catggggaac gccgatatgc ccgctgcccc gatcatcgga
     3601 ttgaccttgc gtttcagaaa caggttgagc gtcttggcga agatgacccc gccggcggtg
     3661 tcgaatatga aggctaccag gcccatcagg acgataaata aggtgctggg ctgaaggaac
     3721 cgatcggcta ccatggtcga gcctatcgcg atgcctagga agagcgtcac aatattggcc
     3781 agctcgttct gggctgactt cgacagtcgc tccacaaccc cggcttcgcg cagaaggttg
     3841 cccagcatca gcgagccgat aagccccacg ctgattggag ctatgatccc ggcgatcacg
     3901 gtgaccatga tgggaaacag cactcgcacg gtcttgctca cccgaaactc ctggtactcc
     3961 atgtgtatcc gccgttcctc gggcgtggtg agcaggcgga tgactggcgg ctggacgagg
     4021 ggtaccagcg acatgtagga gtatgcggcc acggtgatag gccccaataa ctccggagcg
     4081 aatagcccgg acacgtatat agacgttggg ccgtccgccg ctccgatgat accgatagag
     4141 gccgcctgtt tcagggtaaa cgcagggatg aaactagcca caccgtgtaa tctgcccagg
     4201 tacagccctg tcatgagagt cgcgaagatt ccaaactgcg cagcggcgcc gaaaaacagc
     4261 atgaacgggc gcctcagcaa tgggccgaag tctatcatcg cccctacagc gatgaagatg
     4321 aggatgggaa agagttcgtt ctcgataccc gcgcggtata gaatggtcag gaatccatgc
     4381 tctcctatgg cagatgagcc gggtatgttc gtgagaatcg cgccgaaccc aataggtaga
     4441 agaagagtag gttcgtattc cttcttgaca gccaggtaga tcagagctcc tccgatggca
     4501 aacatgaccg cgacacgcca gtagtccacc acgagttgca agccattcac cttccatcca
     4561 cagcgtgaat tgtttcgatt accag
//
LOCUS       DAOTAC010000213         4546 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_15641_length_4546_cov_4.463371, whole genome shotgun sequence.
ACCESSION   DAOTAC010000213 DAOTAC010000000
VERSION     DAOTAC010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4546
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15641_length_4546_cov_4.463371"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1674
                     /locus_tag="PLM74_08990"
     CDS             <1..1674
                     /locus_tag="PLM74_08990"
                     /inference="COORDINATES: protein motif:HMM:NF018528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HON43247.1"
                     /translation="RTASGNECALTMVICAPVGERGFVVRFELSDLVTAAQGQAAQFK
                     VGLTGCWGTTANTIFTRRGMRVMNHAFYSGWTKSLVLEARGVSSLAALAVACNRPMEW
                     TLSGGDGALAKEVQEPGDGSGRIDFSVWTWASLKPGQTWAADFWVAANADPDGAATTS
                     VHLRRVGYQAALEHTLAWLRGRSHRSRIPAEMAGKTRAAISAISRADYNLHFCRFFAH
                     GKAIDTEELVMLTSRSPRYYVSAAFWPRDTFLWAFPAMLASDPAFAREVLLTGFARHV
                     ANMGIHAHYIDGVLLYPGFELDQHASYMVALGSYVTRTGDMSIVSETAVRTGLARFEE
                     ILAKQRHSEAALYRTFLDPSDDPVRYPYLTYDNALLWRAFASLAEIARTAGDSATASR
                     YADTAQRIHTAVCERLVAEGPFGPMFVWSADLEGGFELYDDPPGSLLLLPFYGLIDRN
                     DIRYVNTVKYIMSEHNPYRVAHGDYPGAGCAHAARPWPMHACNMVMAGIADGESLELI
                     AKAPLDGELACESVYEVGGKAATGGAFATFAGYLSMALTQAVTSGIILT"
     gene            complement(1661..3004)
                     /locus_tag="PLM74_08995"
     CDS             complement(1661..3004)
                     /locus_tag="PLM74_08995"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HON43248.1"
                     /translation="MANHTRATEAKNVEIIVLVISVAKWAALAGVTGALGGAAVAAFL
                     MLLEWGVEHAGHIGPWWFMLPVAGLIGAALIHYVSPESYGHGTEAVIRAVNEQDGEIP
                     IRVAPIKALATIISISFGASAGKEGPAAQIAGAVISQAGRALRLAAEDRTRLTMCALA
                     AAFGVAMGAPMAGALFAIEALRMGDPFYVGILPAVIASSVSCIVARRLGWWHPLAAAL
                     PLPGISAVTIAQVIAGAVCLGLVAWLYIEAEEGAARMFHRLRVPPPVRTLIGGIVLVL
                     LTLGFSSEYLGLGEHLIDQALAGASIPLWAFALKILFVAVTLGSGFSGGAMTPLLVIG
                     ATAGNAFGRILGLAPGFGAAIGIAGLLGGAANAPLTAAALGLEAFGPAFGIYGLLAAV
                     VSYTVSGHRSINATQHLGRPKSTAFTVDVGHVCEEHGRVHIDPRIARQFPWLRSK"
     gene            complement(3095..3379)
                     /locus_tag="PLM74_09000"
     CDS             complement(3095..3379)
                     /locus_tag="PLM74_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43249.1"
                     /translation="MLPLIVDTDGDRALLEQMVADACSSGMIDVAVFLMKLMVMDRAP
                     SLYGESTSHLPTEVTCELQRIVREDGWRGIEAIQARMDQMVASKRVGVCR"
     gene            complement(3683..>4546)
                     /locus_tag="PLM74_09005"
     CDS             complement(3683..>4546)
                     /locus_tag="PLM74_09005"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HON43250.1"
                     /translation="GVNIVADFLPADNPIEKHITFSAHYDSKTDIFDHEQRKPIYGFA
                     PIAMGCLLAASMLGIVSSGRKWGPGAHRVCLALACVGVSGLVMLALAFGGGLLVPAGR
                     QSPGARDDASAVAVLLATADQIAADHQQLKRTAVRFVFFGGEEVNMQGSSAYSRSLAG
                     SGEGFDRGVVVNCEFIGGEEPFCYWESSGTFLSKFRASDEAVQLYQDALTSLGLAPAI
                     SAGSIFDDTGRLLAAGFRAVTVGHADPVSSAGYHSQSDSARQVVPAKLQETKSVFLRM
                     ITMTDRTAECL"
BASE COUNT          952 a         1444 c         1286 g          864 t
ORIGIN      
        1 cgcacggcat caggcaacga gtgcgctctc acgatggtca tctgtgcacc tgtgggagaa
       61 cgagggttcg tcgtcaggtt cgagctgtcg gacttggtca cggccgccca gggccaggcc
      121 gcccagttca aggtggggct gaccggatgc tggggcacaa cggcgaatac gatcttcacc
      181 aggcggggta tgcgtgtgat gaaccacgcc ttctactcgg gatggaccaa atcgctagtc
      241 ctcgaggcca gaggtgtatc gagcttggcc gctctggccg tggcatgcaa ccgtcccatg
      301 gaatggacac tcagcggagg cgacggggcg ctggccaagg aggtgcagga accgggagat
      361 ggctccggga gaatcgactt ttccgtgtgg acctgggctt cactcaagcc tgggcagact
      421 tgggcagctg acttctgggt tgccgccaac gccgatccgg acggagccgc cacaaccagc
      481 gtgcacctaa ggcgcgtcgg ctaccaggcc gcgctggagc acaccctcgc ctggttgcga
      541 ggtaggtcgc accgcagtcg catcccggcc gagatggccg gaaagacccg ggccgcaata
      601 tccgcgatat ccagggccga ttacaacctg cacttctgcc ggttcttcgc ccacggcaag
      661 gcgatcgaca ccgaagagct ggtgatgctg acctcacgca gcccgcgata ttacgtctcg
      721 gccgccttct ggccccgcga cacgtttcta tgggcatttc ccgccatgct tgcgtccgac
      781 cctgccttcg cccgggaggt gttgctcaca ggcttcgcaa ggcacgtcgc gaacatgggg
      841 atccacgcgc actacattga cggcgtattg ctctaccccg ggtttgagct ggatcagcac
      901 gcatcctaca tggtggcgct gggcagttac gtgacgcgca ccggcgacat gtccatcgtc
      961 tcagagacgg cagtgcgcac agggctagcc agattcgaag aaatcctggc caagcagcgc
     1021 cattcggagg cggccctgta ccgcacgttc ctcgacccca gcgacgatcc tgtcaggtac
     1081 ccctatctca cctacgacaa tgccctgctc tggagggcgt tcgcgtctct cgcggagatc
     1141 gcgcgaacag ccggtgacag cgctacagcc tcaagatacg ccgacacggc gcagagaatc
     1201 cacacagccg tctgtgagcg gcttgtagca gaaggcccat tcgggccgat gttcgtgtgg
     1261 tccgcagatc tcgaaggcgg attcgagcta tatgacgacc cgccgggaag cctcttgctt
     1321 cttccatttt acgggttgat cgatcgcaat gacatccgat acgttaatac agtcaagtac
     1381 ataatgtcgg aacacaaccc ctaccgcgtc gcacatggcg actaccctgg agccggatgc
     1441 gcccacgctg ctcgcccctg gcccatgcac gcctgcaaca tggtgatggc cggcatcgca
     1501 gatggcgaat cgcttgagct gatagccaaa gctcccctag acggggagct tgcatgcgag
     1561 agtgtctatg aggtcggcgg aaaagcagct acaggcggtg ccttcgctac atttgccggt
     1621 tacctatcga tggccctaac gcaggccgtc acgagcggca tcattttgac ctaagccacg
     1681 gaaactgcct cgcgatgcga ggatcgatat gcacgcgccc atgctcctcg cacacatgcc
     1741 cgacatctac ggtgaaagcg gtagacttcg ggcgccccag atgttgcgtg gcattgatgc
     1801 tgcgatgacc tgacacggtg tatgatacga ctgccgccaa gagcccatat atcccgaacg
     1861 ccggcccgaa ggcctctagg ccaagggctg ctgctgtgag cggagcattt gccgcgccgc
     1921 ccagcaggcc ggctattccg atggccgcac cgaaccccgg tgcaaggcca agaatcctcc
     1981 cgaaggcatt ccccgcagtt gcgccgatca ccagaagcgg ggtcatggca cccccgctga
     2041 aacctgatcc caaggtgacc gcgacgaaca gaattttgag cgcgaatgcc cagagcggga
     2101 tggacgctcc cgctaaggcc tgatcgatca ggtgttcacc gaggccaagg tactcgctgg
     2161 agaagcccag cgtaagaagg acgaggacga tgcctcctat aagcgttctg accggaggcg
     2221 gcacgcgcag cctgtggaac atgcgtgcag cgccttcctc cgcttcgatg taaagccatg
     2281 ctacgagacc aaggcagacg gcgccggcta tcacttgcgc aatggtaacc gcgctgatcc
     2341 ccggcaacgg cagcgccgca gcgagcgggt gccaccatcc aagccggcgg gcgacgatgc
     2401 aggataccga ggacgcaatc acggcaggca ggatccccac atagaacgga tcgcccattc
     2461 tcagtgcctc tatcgcgaac agagcccccg ccatcggagc gcccatcgcg accccgaagg
     2521 cggcggccag agcacacatg gtgagcctgg tgcgatcctc cgccgcaagt cgcagggccc
     2581 gcccggcctg agaaatgaca gcacctgcta tttgcgctgc aggcccttcc ttccctgccg
     2641 aagccccaaa agagatgctg attatggtcg ctaatgcctt aatcggcgcg acgcgtatcg
     2701 gtatctcgcc gtcctgttcg ttcacagccc gtatcacggc ctctgtaccg tgaccgtatg
     2761 attccggaga cacgtaatgg ataagtgccg cgccgatcaa tcctgcaacg ggaagcatga
     2821 accaccaggg gcctatgtgc ccggcatgtt ccacccccca ttccagcagc atcagaaaag
     2881 cagccaccgc cgcaccgccc aaagcgccgg tgacaccggc aagtgctgcc cacttggcca
     2941 cggagatgac aagcacgata atctcgacat tctttgcttc ggttgctcgt gtgtggttcg
     3001 ccaatcatga ccaacctttc gttcatcaat cttggatgcc atgctacatc ctccgcgcaa
     3061 gtcaccaagc ggtacagtcg cccggctgcc acgcctagcg acatacccct actctcttcg
     3121 aagccaccat ctggtccatt ctggcctgga tggcctcaat cccacgccat ccatcctcgc
     3181 gcactattct ctggagctca caagtaacct cagtcggcag atggctggtg gactctccat
     3241 acaggctggg agcacggtcc atcaccatta gtttcatcag gaacaccgcg acgtcgatca
     3301 ttcccgatga gcatgcatcc gcaaccatct gctccagcaa agcacggtcg ccgtccgtat
     3361 caactatcag cggtaacacc tcccctctga gactagttcc aacgaattac ttctcttcca
     3421 tcttaccctg cttttggtga ttagtaaaga tggatttttg ccgaaacgtg taggatcttc
     3481 tagtcgatgt tatcctgtgt tatgcaggcg cttggggtgg tcgctaccca cagagtgccc
     3541 ggcgcaattg tgtgacaatg tttgtaattc gtcgctagct atggcatgca ttgtatggac
     3601 gatatggtgc tatcgctgca gaacgccagc gagttgcaca gacgaaagcc ttgcacaact
     3661 gcactccgcc aagaggactt ctctacaaac actccgctgt tcggtctgtc atggtgatca
     3721 tccgaaggaa cacgctctta gtctcctgca gttttgcggg aacaacttgg cgcgcgctgt
     3781 ccgattgact atggtatccc gccgatgaca cgggatcggc atgtcccacc gtcactgccc
     3841 taaacccggc cgcaagcaaa cgacccgtat catcgaatat ggatccagcc gatatcgcag
     3901 gcgcaagccc cagagaggtc agggcatcct ggtagagttg tactgcctcg tctgacgctc
     3961 ggaattttga cagaaaagta cctgagcttt cccagtagca gaagggttcc tcaccgccga
     4021 tgaactcgca gttgacgaca acgcctcgat caaacccttc gcccgatccg gctagtgacc
     4081 tggaatatgc gcttgacccc tgcatgttga cctcctcgcc cccgaagaac acaaatcgaa
     4141 cagcagtgcg cttaagctgc tgatgatcgg ctgcgatctg gtcggccgta gccagaagca
     4201 ctgccaccgc gctcgcatca tcgcgggctc cggggctctg gcggccggcc ggtacgagca
     4261 gaccgccccc aaatgccagg gctagcatga cgaggcccga tacgcccaca cacgccagcg
     4321 cgaggcagac cctgtgcgcc cccggccccc actttcttcc ggaggagact atcccgagca
     4381 tcgatgcagc aagcaagcag cccatggcga tcggggcgaa gccgtatatc ggcttgcgtt
     4441 gctcatggtc gaagatgtcg gtcttggagt catagtgcgc cgagaaggtg atatgcttct
     4501 cgatggggtt atcagcaggt aggaagtcgg ctactatgtt gacccc
//
LOCUS       DAOTAC010000214         4527 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_15710_length_4527_cov_3.838998, whole genome shotgun sequence.
ACCESSION   DAOTAC010000214 DAOTAC010000000
VERSION     DAOTAC010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4527
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15710_length_4527_cov_3.838998"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="PLM74_09010"
     CDS             <1..539
                     /locus_tag="PLM74_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HON43251.1"
                     /translation="NIAHNVLDRLGYPTRSAELAGIAGYLHDIGNAVSRDHHGQSSAL
                     MTMSILSRMGMDPTEIALIMAAVGNHEEEYGHPVNEISAAVILADKSDVHRTRVRKKD
                     IPTMDIHDRVSYAAERSFLDVDAEKRVITLTLTIDTGICPVMEYFEIFLTRMTMCRRA
                     ASVLECEFKLEINGACLL"
     gene            635..2221
                     /locus_tag="PLM74_09015"
     CDS             635..2221
                     /locus_tag="PLM74_09015"
                     /inference="COORDINATES: protein motif:HMM:NF036831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43252.1"
                     /translation="MRSYESLVKHEGNSALFAAIEISIVSTLAGRPVHVHAEGVRGTG
                     KTTIMRAAAGILPMIERITGCDHNCRPWAPHCPDHVSAPPARLREIGSEFVQMPFLEI
                     SHSARLGTVVGSIDLARVVDPSNPQAALLLGTIPRANRGIIFIDEINRLADTSPELTD
                     VLLDTMGTKPGRVQIEEAGLPVVSLPLETGVWAASNPDEDPGPLEDVRRQLSDRFDLS
                     VEMGRACEPGMIRRILECDDFDVPRRAPEAPTAGCWPGRMAAAALLAPRVLFPDEMVE
                     LLATIYVDFDIESIRALEAARTAARCRAALAGRSRVSIEDLRAVLGMVLRHRVEVAAL
                     ANILRYVDAMQEKSAHGRSVGAVMPGCPGGPGRAGTQGAEGTRPDRDGGPDRADNDCA
                     CDRGRSGDGGRVDMDQASAHGGAGLRGILHRLFEHMRSEQHGGSCGPECAGRLDSQGP
                     SGSRGDSAGGPDGGYAGGVGTGIGACGRRRQGAGTGAGAGTGSACAPESTVSEVCPPS
                     IARPITQVGPKVVFAEEDLI"
     gene            2218..3933
                     /locus_tag="PLM74_09020"
     CDS             2218..3933
                     /locus_tag="PLM74_09020"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HON43253.1"
                     /translation="MKPDELATFAAEVNCRLDCGHGVRVGETALVSRRVHVIDPTRPY
                     EARVKCAADAGQVFYRRSLADEVVHVDIFHSLADVSHYAIAQASRQAVDEFSRQASLG
                     ISCSAALADFIDVQTATGGGRATGSLKYGQKPSLQTAHRGHVHLAALLPREASGLLYY
                     LVRNAEQAIQRQGAELRRLDRIVNDATHGGAPIDLSPYSCPTDSLLQEHAPPARLTDD
                     CAMMKDALDAAADFGSVSSLASFLECVAAARRRDNPYSLLEREYGDAESMLSRLRALD
                     ILSGDGASLVLTDKGIQMASFLRRFAREMETQMRKVIRRSGRPRVIPLDKSDFTRGRT
                     RGPSAKESRAIMSAEEADFRGAISVPGTIGQWAVRCSQQGRWGPIEPCDVRVEQARRR
                     LRVDVCLLIDASASMAGKRIQAAKHLARYMFLTCRDRVSVLTFQDRNVTVHVVRARSH
                     RALEQGLSTVRPAGLTPLAAGIVEAVRLLGARHHAPAMLVLLTDGIPTMNQWTGDPAR
                     DALTAAQQIAENNIPFTCIGLAPNKGFLRRLTEIACGTLYIVDEFDRDVLAKLVKDER
                     SRVQT"
     gene            4000..>4527
                     /locus_tag="PLM74_09025"
     CDS             4000..>4527
                     /locus_tag="PLM74_09025"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HON43254.1"
                     /translation="MSQKKKTWELVAADPRVAAELSARCSISLGMAKVLANRGIVDET
                     SARRFLAPDITNMHDPFALPDMQAAAGRLLDAVRQGEKIRVYGDYDVDGISSVSLVVS
                     VLRACGADVDYYIPNRLIEGYGLSIEAVQKGAEDGVGLLVTADCGIGAFDEAEAAQSC
                     GVDLIITDHHEAGASL"
BASE COUNT          879 a         1302 c         1498 g          848 t
ORIGIN      
        1 ccaacatagc ccacaatgtc ctggaccggc tggggtatcc cacgcgctcc gccgagcttg
       61 ccggcattgc cggctacctg cacgacatcg ggaacgcggt gtcccgcgac catcacgggc
      121 agagcagtgc tttgatgacc atgtcgatac tatcgcgcat ggggatggac ccgactgaga
      181 ttgcgctgat catggcggcc gtcggcaacc atgaggagga gtatgggcat ccggtgaacg
      241 agatctctgc ggctgtgata ctcgctgaca agagcgatgt gcatcgcacc cgtgttcgca
      301 agaaggacat ccctaccatg gatatccacg acagggtgtc gtacgcagct gagcggtctt
      361 ttctggatgt ggatgccgaa aaacgggtga ttacgctcac gctcaccatc gataccggca
      421 tctgtccagt aatggaatac ttcgaaatct tcctgacccg gatgaccatg tgcagaaggg
      481 cggcttcagt ccttgaatgc gagttcaagc tggagataaa cggcgcatgc ctgctgtagg
      541 cgcacggcgc tagcaaagcc ccagctagca gaatcaaacc ctccgcacgg tgcaatacta
      601 ctctggacac tgcaccgatg cggaggggtt cgttttgcgc tcgtatgaga gcctcgtgaa
      661 acacgaaggc aactcagcgc tattcgctgc gattgagata tctatcgtat ctactctggc
      721 agggcgcccg gtgcatgtgc acgccgaggg tgtcagggga accggcaaga ccaccatcat
      781 gcgggcggct gcaggtatcc tgcccatgat cgagagaatc accgggtgtg atcataattg
      841 taggccttgg gcgccgcact gccccgacca cgtaagtgcg cctcccgctc ggctgcggga
      901 gataggctcg gaattcgtgc agatgccttt cctggagatc tcgcactcag ctaggctggg
      961 aacggtcgtg ggcagcatag acttagcgcg cgtcgtcgac ccgagcaatc cccaagctgc
     1021 tctgcttctt ggtacgatac cccgggcgaa ccgcgggata atcttcatcg acgaaatcaa
     1081 caggctcgca gacacgtcgc cggagctgac ggatgtgctt cttgacacca tgggcaccaa
     1141 accaggcagg gtgcagatag aggaggctgg cctgccggtg gtgtcgctcc ctctggagac
     1201 gggcgtgtgg gcggcatcca accccgatga agaccccggc cctcttgagg atgttcggcg
     1261 tcagctgtct gaccgattcg acctctcagt cgaaatgggc cgggcgtgtg aaccgggcat
     1321 gatccgcagg atcctggagt gcgacgactt cgacgttccg cgacgagcgc cggaggctcc
     1381 gacggccgga tgctggccag gccgcatggc cgccgccgcc ctgcttgcac cgcgggtgct
     1441 cttccccgat gagatggtgg aacttctcgc tactatctac gttgacttcg acatcgaaag
     1501 tatccgcgca ctggaagcgg ccaggacggc tgcgcgctgc agggctgcac tggccggtag
     1561 gagccgcgtg tctatcgagg acctcagagc agttctcggc atggtgctgc gccaccgcgt
     1621 agaggtggcg gcccttgcca atatccttag gtacgtcgat gcgatgcaag aaaagtcggc
     1681 tcacggtagg agtgtgggcg ccgtcatgcc tggatgccca ggagggcccg ggcgggcggg
     1741 cacacagggg gcggagggga cgcggccgga ccgcgatggc ggcccagacc gggccgacaa
     1801 tgactgcgcc tgcgaccgcg gccggagcgg cgatggcggc cgcgtcgaca tggaccaggc
     1861 ttctgcgcat ggcggggcgg ggctccgtgg catattgcac aggctcttcg agcacatgcg
     1921 ttctgaacag cacggtggct catgcgggcc ggagtgcgca ggccgcctgg attcacaggg
     1981 gccgagcggt tcccgcggcg attcggcagg cggccctgac gggggctacg ctggcggggt
     2041 ggggacaggg atcggcgctt gcggtcggcg gcgacaaggt gcgggtacag gcgcaggggc
     2101 cgggactggt tcggcatgtg ccccggaaag cacggtcagt gaggtgtgcc ctccgagtat
     2161 tgcccggccc atcactcagg tggggcccaa ggtcgtgttc gctgaggaag acctgatttg
     2221 aagcctgatg agcttgcaac cttcgcggca gaggtcaatt gccgactcga ttgtgggcat
     2281 ggagtgcgag tgggcgagac cgctctggtc tcgcgccgag tgcacgtaat cgatccgact
     2341 cgtccatatg aagctcgggt gaagtgtgct gccgatgcgg ggcaagtctt ctaccgccgc
     2401 agcctcgccg atgaggtagt ccatgtcgat atcttccatt cactggccga tgtcagtcat
     2461 tacgcgatcg cccaggcatc caggcaggcc gttgacgaat tctcgcgcca agcatccctg
     2521 ggcatcagct gttcggcagc gttggcggac ttcatagacg tccagaccgc tacgggcggc
     2581 ggcagggcaa cgggcagtct caaatacgga cagaaaccgt ctctccagac ggcccacaga
     2641 ggacacgtac atctagctgc gctcttgccg cgcgaggcct cagggttgct ctattacctt
     2701 gtgcgcaacg cagagcaggc tatacagcgg caaggggcgg aactgaggcg gctggaccgg
     2761 atcgtaaatg atgcaaccca cgggggggct ccaatcgatc tgtcgcccta ttcatgtcca
     2821 acagactcgc tcctgcagga acatgctccg ccggccaggc ttactgatga ctgcgcgatg
     2881 atgaaggatg cactcgatgc agcggccgat ttcggatctg tgtcgagcct ggccagcttc
     2941 ctggagtgcg tggcggcggc gcgtcggcgg gataatccct attcactcct cgaacgcgag
     3001 tacggtgatg cggagtcgat gctctctcgc ctgcgggcac tggatatact cagcggcgac
     3061 ggcgcttctt tggtgttgac cgacaagggg atacagatgg cctcatttct gcggcggttc
     3121 gctcgcgaaa tggagacgca gatgcggaag gtgatccgac ggagtgggcg acctcgggtc
     3181 attcccctcg acaagagcga cttcacgcgc ggcagaaccc gtggcccatc cgcgaaagag
     3241 tcgcgcgcca taatgtcggc tgaagaggcc gatttcaggg gcgcgatatc cgtgcccggc
     3301 accatcggtc agtgggctgt tcgctgttcg cagcagggac ggtggggtcc gatcgagccg
     3361 tgcgatgtcc gggttgagca ggcgcgacga cgcttgaggg tggatgtgtg cctcctgatc
     3421 gatgccagcg ccagcatggc cgggaagcgc atccaggcgg caaagcacct ggccaggtat
     3481 atgtttctca catgccgcga tcgcgtgagt gtattgacgt tccaggaccg caacgtcacg
     3541 gtgcatgtcg ttcgcgcccg ctcacaccgg gctcttgaac aaggtctgtc tacagtcagg
     3601 ccggccgggc tcacgcctct ggctgcaggc attgtggaag ccgtgcggct tctgggggcg
     3661 aggcatcatg cccctgccat gctggtactg ctgacggacg ggatacctac gatgaaccag
     3721 tggacgggcg accctgcacg ggacgcactt acggcggcgc agcagatcgc tgagaacaac
     3781 atacccttta cgtgcatcgg gctcgccccg aacaagggct tcctgcgccg gctcactgag
     3841 attgcctgcg gcacactgta catcgtagac gagttcgacc gcgatgtact ggcgaaactc
     3901 gttaaggacg agcgcagcag ggtgcaaaca tgatactata ggcaatagaa gggcaaacgg
     3961 tcatcaggtc gaattgtctc tgactaaggt ggagaataga tgtcgcagaa gaagaaaaca
     4021 tgggagctgg tcgcggcgga cccgcgcgtc gcagcagaac tgtccgcccg ttgtagcatc
     4081 tcgctgggca tggccaaggt ccttgcaaac aggggcattg tggatgagac aagcgccagg
     4141 cgctttcttg cgccggatat caccaacatg catgatccat tcgcgctccc cgatatgcag
     4201 gccgccgccg gacggttgct tgacgcggtt cgccagggcg agaagatcag ggtatatggc
     4261 gactatgacg tggacggaat atcgtctgtg tctttagtcg tatcagtgct tcgtgcctgc
     4321 ggcgcagatg tggactatta catccccaac cgtctgattg aggggtacgg cctgagtatt
     4381 gaggcggtcc agaagggggc agaagacgga gtagggctgc ttgtcactgc cgactgcgga
     4441 atcggggctt tcgacgaggc agaggcagcg cagtcgtgcg gcgtagacct gatcatcacc
     4501 gatcatcacg aggccggtgc tagcctg
//
LOCUS       DAOTAC010000215         4497 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_15845_length_4497_cov_4.849617, whole genome shotgun sequence.
ACCESSION   DAOTAC010000215 DAOTAC010000000
VERSION     DAOTAC010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4497
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15845_length_4497_cov_4.849617"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..161)
                     /locus_tag="PLM74_09030"
     CDS             complement(<1..161)
                     /locus_tag="PLM74_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013290054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HON43255.1"
                     /translation="MQGELPLENIRNIGIMAHIDAGKTTTTERILFYTGRIHRLGEVD
                     AGTATMDWMV"
     gene            complement(205..675)
                     /gene="rpsG"
                     /locus_tag="PLM74_09035"
     CDS             complement(205..675)
                     /gene="rpsG"
                     /locus_tag="PLM74_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014792380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HON43256.1"
                     /translation="MPRRGKVTRSEVSPDPIYGSMLVSEFINKLMLSGKKGTAEAIFY
                     GAAEIIQSKTGKEPLETFDRALKNVMPVLEVRPRRVGGATYQVPIEVRPERRKALALR
                     WIVTNARKRPEKTMSERFAGEILDAAAGQGTSIKKREDMHKMAEANKAFAHYRW"
     gene            complement(709..1122)
                     /gene="rpsL"
                     /locus_tag="PLM74_09040"
     CDS             complement(709..1122)
                     /gene="rpsL"
                     /locus_tag="PLM74_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009448792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HON43257.1"
                     /translation="MPTINQLVRKGRQKASTKSMAPALRYQYNSLNRRTVYGEGSPQR
                     RGVCTVVRTTTPKKPNSALRKIARVRLSNGLEVTAYIPGVGHNLQEHSVVLIRGGRVK
                     DVPGVRYHIIRGTLDAAGVANRRQGRSKYGAKRPK"
     gene            complement(1206..1454)
                     /locus_tag="PLM74_09045"
     CDS             complement(1206..1454)
                     /locus_tag="PLM74_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HON43258.1"
                     /translation="MYEDLASAPSRVIGVKQTVRALERGVVTAVYVARDAEERVVREL
                     KALAAAKGIPVNPVESMDELGKVCGIDVGAAAAAILHE"
     gene            complement(1796..4497)
                     /gene="rpoC"
                     /locus_tag="PLM74_09050"
                     /pseudo
     CDS             complement(1796..4497)
                     /gene="rpoC"
                     /locus_tag="PLM74_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457901.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
BASE COUNT          781 a         1502 c         1195 g         1019 t
ORIGIN      
        1 accatccagt ccatcgtggc cgtgccggca tcgacctcgc ccagacggtg gatgcggcca
       61 gtgtagaaga gaatacgctc agtggtagtc gtcttccctg catcaatgtg agccattatc
      121 cctatgtttc tgatgttctc tagaggcaac tcaccctgca tatctcttcc ctcctctcct
      181 gccccgacag cccggatagc gggcttacca cctgtagtgc gcaaaggcct tgttcgcttc
      241 ggccatcttg tgcatgtctt ctcgcttctt gatagaggtt ccctgcccgg ccgctgcgtc
      301 tagaatctcg ccggcgaatc gctcgctcat ggtcttttca ggacgcttcc tggcattagt
      361 cacgatccac ctcagagcca aagccttccg cctctcgggc ctgacctcaa tgggtacctg
      421 atacgtcgcg cctcctaccc ttcgaggcct gacttcgaga accggcataa cattcttgag
      481 cgcacggtca aaagtctcta gaggctcttt tcccgtcttg ctctgaataa tctcggcggc
      541 cccgtaaaag atggcctcag cagtgccctt cttgccggaa agcatcagct tgttgatgaa
      601 ctccgacacg agcatggacc cgtagatcgg gtcaggagac acttcacttc ttgtaacctt
      661 gcctcgcctc ggcatagctc accctcccct cgctatctcc tgggagcgtc acttcgggcg
      721 ctttgctccg tatttggagc ggccctgacg tctatttgca actccagcag catccagagt
      781 gccgcggata atgtggtacc tcacgcccgg cacgtccttt acacggccgc ctctgatcaa
      841 gacgaccgag tgctcctgga ggttatgccc tacgcctgga atgtaggccg tcacttcgag
      901 tccgtttgac aacctcaccc ttgcaatttt cctgagagca gagttgggct tcttcggcgt
      961 agtcgtcctc accactgtgc atactcccct gcgctgtggt gatccctcgc catagacagt
     1021 cctccgattg agcgagttgt actggtacct gagagcaggc gccatagact tggtcgaggc
     1081 cttctgcctt cccttcctta ccagctgatt aatggttggc acatgcgcca cctccttccg
     1141 caagactgca acatttcgga cgcaacatgc cccttccacc tattttcagg tgcaaggggc
     1201 acgattcact cgtgtagtat ggcggctgcc gccgcgccca catctatccc gcacaccttg
     1261 ccaagttcgt ccatggattc aaccgggtta accggaatgc ctttcgcggc agcgagcgct
     1321 ttcagttcac gcacgacacg ctcttcggca tcgcgggcca cataaaccgc cgtgactacc
     1381 ccgcgctcta aggctctcac cgtctgcttg acgccgatga cccggctcgg ggcagaggcg
     1441 aggtcctcgt acatgaacgg ctttcgcccc tttcagcctg gccttccacg cactacgata
     1501 ctggctcaga cagttttaca ttttaccatc agccgcggga cgtgtcaagg gcacccgagc
     1561 cctcgaaatc cctgcatcag gagtcggttg ctgcgttcac atggcccagc gcagatgctt
     1621 gagcccgatc tagatgctcc gccatgggcg ctccgaaatg acgatctgcg cccgccgcaa
     1681 gcgccccgac cgcaccgccg atcccggcgg ctggggccgc ctcaagtcgg gctcgatcat
     1741 ccgtaaccgg cgccgtcatc agctcagcct cagcctccgg ggcaaccttg atccggatgt
     1801 tgcggtatct gctcatcccc gttcctgccg gtatgagctt gcctatgatg acattctcct
     1861 tcaagccgat gagcgggtct gtcctgccct ttatggatgc atccgtcagt acgcgcgtcg
     1921 tctcctggaa tgacgcggcc gagaggaagc tatctgtctg aagcgacgcc ttggtgatgc
     1981 ccagcagaat cggcttggac gtagcggttc tcaagccgcc ggcctcggcc tcttcgttgg
     2041 tctcctcgaa tctgaacacg tcgatggtct cccccggaag catgctggtt tcgcccggat
     2101 cctccacctt gaccttgcgc atcatctgcc tcacaacgac ctcaatgtgc ttgtcgttga
     2161 tctccacacc ctgcgagcga tatacatcct gcacctctcg cacgatgtac tgctggactg
     2221 ccaccggccc acgtatgcgc agtatgtcat gggggttcag cgacccctcg gtgagcttgt
     2281 cgccggcgac cacacggtcg ccctcgcgca cctcaaggcg cgcgccgaac ggcaccgagt
     2341 actcgtgctc ctcatcggac tcggtggtga ctatgacctt tcgggctccc ttgacctcgg
     2401 ttatgcgaac cacaccgtcg tactccgtga cggtggcaag gcctttcggc ttcctcgcct
     2461 cgaacagctc ttcgacgcgg ggcagacccc gtgtaatgtc gtcgcctgcg actccgccgg
     2521 tgtggaacgt cctcattgtg agctgcgtgc cgggctcgcc gatggactgc gcagcgatag
     2581 tgcccacggc ctcgcccact tcaaccaggc tccctgtagc gaggttccgc ccgtagcact
     2641 tggcgcacac accgtacctg gtgcggcact tgagaaccga gcggatgggc caggagttcg
     2701 ggccgccatg cttgaccttt tcgtcgacct gacgctcgat ctcgcttgcc gtgtcttcat
     2761 ctatcatctg gccggcctct aggagcacct cgcctgtctc ctggttgatc aggtcgctca
     2821 agctgaccct tccggttatg cgttcagcca gagtctcaat gacggtgtcg ccttccatga
     2881 gcgcggtagc ttctattccc tcatcggtgc cgcagtcgtc ctcgcggact atcacatcct
     2941 gtgccacgtc cacaaggcgc ctagtcaggt agcctgagtc ggccgtcctg agggccgtgt
     3001 cggccaggcc cttccgcgtg ccgtgtgtgg agatgaagta ctccagcacg gtcaagccct
     3061 cgcggaagtt ggctttgata ggcacgtcga tggtccgtcc cgacgggtcg gccaccagac
     3121 cgcgcatgcc ggccagctgg ttgagctgct gcacgttacc gcgggcgccg gatgtagcca
     3181 tcatgtagac cgggttgagc tggtccacag tgaactcctc gggtctatcc gcgccgcgtt
     3241 ccctggcctc ctcgaccttg tccttcatga tctcctcagg gttgaggtgc ctcatcatgg
     3301 ccttctggac cttctcacgc gcctcggtcc atacgtctat caccaggctg tacttctcat
     3361 cctcgagcag caggccccgc ctgtactggc cttcgatctg ttcgactttg gtctcagcct
     3421 cgcgtatgat ctccgtcttc tcctcaggga tcgcgatgtc tcctattccc acagtggtgc
     3481 cggaaaccgt agcgtagtgg aagcccaggc gctttaaggc gtccaaaaca tcggccgtca
     3541 tcgcattgcc gctcttccgc ctgatcctgc ccactagttt gctcaaaaag ctcttgcttg
     3601 caggcttcct ggttcgtccg ccgccaatcg acagaaactc atcgaacgac atgcctcggg
     3661 gcacgaaatc gccggcggtg agcatgtcgt cgaaagtggc ctcggccgag ttgatatacg
     3721 gaaaatcatc ggggaaaatc tcgttgaaca cgagcttacc gacggtcgtt actattctgc
     3781 gcgtacctgc ttcctccgac cccagcacat tcttgctttc gggcatcctc accagtatcc
     3841 gcgcatgaag cgcaaccacg cctgcatcgt aggctgcctt ggcggcttcg gggttttcaa
     3901 agatcttacc ttcgccgagg gcgcctatgc gctcgaacgt caagtaatag cagcccagga
     3961 ccatatcctg cgtgggcgta gccacagggc ccccgtgagc cggtgagagt atgttgttga
     4021 ccgccagcat gagcagcctg gcctctgact gagcctcagc tgacagcggc acatgcacgg
     4081 ccatctgatc gccgtcgaag tccgcgttgt acgcagtgca caccagcgga tgtatctgga
     4141 tcgccctgcc ctcgaccagt acaggctcga aagcctgtat tcccagcctg tgtagggtag
     4201 gggcgcggtt aagcagcacc gggtgctcgg cgatgatctc ctcgagcacg tcccatacct
     4261 ccggcctgac tcgctccacc attcgcttgg cgctcttgat gttgtgcgca agcccccgat
     4321 ccaccagccg cttcatgacg aacggcttga acagctccag ggccatctcc ttaggcaggc
     4381 cgcactggtg catcttcaga gtggggccga ccacgatgac cgaacgcccc gagtagtcaa
     4441 cgcgcttgcc caaaaggttc tggcggaaac gcccctgctt ccccttgaga agatcgg
//
LOCUS       DAOTAC010000216         4491 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_15875_length_4491_cov_5.308161, whole genome shotgun sequence.
ACCESSION   DAOTAC010000216 DAOTAC010000000
VERSION     DAOTAC010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4491
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15875_length_4491_cov_5.308161"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /gene="meaB"
                     /locus_tag="PLM74_09055"
     CDS             complement(<1..827)
                     /gene="meaB"
                     /locus_tag="PLM74_09055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HON43259.1"
                     /translation="MDWQEIVSRALARDIRAVSRLISIVEDRRSGFENAYAAIYPHTG
                     RAHVIGITGPPGSGKSTLVDKMAAEYRGRGKTVGIVAVDPTSPFTGGALLGDRLRMQR
                     HSLDDGVFIRSMGTRGHLGGVSRATSGVVDVLDAAGYDYVLIETVGVGQSEVEIASLA
                     DTVALVQVPGLGDDIQVIKAGVMEIGDVFVVNKKDRPGVERVVAEIRMALDLAGNASG
                     RVRAYSGHHGPTAASAAGAVATTEVTPWRPPILQTMAESGEGVAEVCDMCDVHMCRLT
                     "
     gene            complement(836..1237)
                     /locus_tag="PLM74_09060"
     CDS             complement(836..1237)
                     /locus_tag="PLM74_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HON43260.1"
                     /translation="MDEGRKIRVLVAKPGLDGHDRGAKVVARALRDAGMEVIYTGLRQ
                     TPEQIVAAAIQEDVDVVAMSILSGAHNYLFPRVMQLLRERGVDDVLVVGGGIIPDEDI
                     PGLKECGIAEVFTPGASTADTVKFIRENIKR"
     gene            complement(1257..2924)
                     /locus_tag="PLM74_09065"
     CDS             complement(1257..2924)
                     /locus_tag="PLM74_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HON43261.1"
                     /translation="MNQGDLSGEYGRWKSGPYAKTCARFPERKERFEGSSGIEVKPLY
                     TPLDVEQVDYVNDLGFPGEYPYTRGVQPTMYRGRFWTMRQYAGFATAEESNRRYKYLL
                     EQGQTGLSVAFDLPTQIGYDSDHELSAGEVGKVGVAIDSLADMETLFDGIPLDKVSTS
                     MTINAPASMLLAMYIAVAEKQGVFANKLNGTIQNDILKEYAARGTYIFPPKPSMRLIT
                     NIFEYCSTAVPRWNTISISGYHIREAGATAVQEVAFTLADGIAYVKAAVDAGLDVDDF
                     GPRLSFFFNAHNDLLEEVAKFRAARRLWARIMRDRFGAKDPKSMQLRFHTQTAGCTLT
                     AQQPDNNIVRVTLQALAAVLGGTQSLHTNSRDEALALPSQDSVRIALRTQQIIAYESG
                     VADTIDPLAGSYYIEALTDEIERAAADYIRRIDEIGGAPAAIERGFIQAEIEESAYRY
                     QMEIESGDRTIVGLNDFQIEEDRPSGLLKVDPAVGDLQCRKLAQLRAERDNAAVEASL
                     AALEHAASGSDNLMPYILDAVRAYATLGEMCGVLRGVFGEYKPTVAY"
     gene            complement(2989..>4491)
                     /locus_tag="PLM74_09070"
     CDS             complement(2989..>4491)
                     /locus_tag="PLM74_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HON43262.1"
                     /translation="TVAVEQVMPGDIVIVRPGERVPVDGEIIEGYTSIDESMLTGESI
                     PVDKGAGDAAVGGTVNQHGMFKMRATHVGADTALAQIVRLVEEAQGSKAPIQRLADVV
                     ASYFVPTVLAIAAVTFVGWHFAGVGIAQALVNATSVLVIACPCALGLATPTGIMVATG
                     RGAELGVLIRGGEHLERLSRIDAVVVDKTGTLTQGKPRVVDSKVFLSLKPGEALRLAA
                     AAERASEHPLGRAIVEEADLQGLNAPMPTDFRSVPGRGVTAQVDGRQVAVGTLAFLSD
                     LGVDVADDVVRALEHMEEKGETGVLQAVDGTVTAAYGIADQLRPESAGVIAELFGLGL
                     EVYMITGDNARTAAAIARQVGIPDENVMAEVLPDGKAEQVEKMRRRGLHVAMVGDGIN
                     DAPALATADVGIAMSAGTDIAMEAADITIVGGLHRLPDAIRLSRATLGIIKQNLFWAF
                     IYNAVGIPLAAFGIMPPIYAGAAMAASSVSVVSNSLRLKRFDAASSVVKR"
BASE COUNT          841 a         1530 c         1224 g          896 t
ORIGIN      
        1 gtaaggcggc acatgtgcac atcgcacatg tcacatactt ctgctacgcc ttcgcctgac
       61 tccgccatgg tctgcaaaat gggcgggcgc cacggagtaa cttcagtcgt agcaactgct
      121 cccgctgccg atgcggccgt cggcccgtga tgccccgagt acgccctgac gcgtcctgac
      181 gcatttccgg ccaggtcgag cgccatgcgt atctcggcaa ccaccctctc aacgcccggc
      241 cggtccttct tgttgaccac gaacacgtcg ccgatctcca tgacgccggc cttgatcacc
      301 tggatgtcgt ctccaagccc aggcacttgc acaagagcca ccgtatcggc aagtgaagcg
      361 atctccactt ccgactggcc cacgcccacg gtctcaatga gcacgtagtc gtacccggcg
      421 gcatcgagca catccaccac gcccgatgtg gcccgcgaca ctccgcccaa atgcccgcgc
      481 gtgcccatgc tcctgatgaa caccccgtcg tccagcgagt ggcgttgcat gcgcaaccta
      541 tcgccgagga gcgcgccgcc tgtgaacgga ctggtggggt ctaccgccac gatcccgacg
      601 gtcttgcccc gcccgcggta ctcggccgcc atcttgtcaa caagcgtgct cttgcccgat
      661 ccggggggac cggtaatgcc aatgacatgg gccctgcccg tgtgagggta tatcgcagcg
      721 tatgcgttct cgaatcctga tcgacggtcc tccacgatgg atatgagccg ggacaccgcg
      781 cggatgtcgc gtgccagcgc ccggctcaca atctcctgcc agtccattcc gatgcctacc
      841 tcttgatgtt ctcgcgtatg aatttcaccg tgtcggcggt cgaggccccg ggagtgaaca
      901 cctccgcgat gccgcactcc ttaagccctg ggatatcctc gtcgggtatg atgccgccgc
      961 ccaccaccag cacatcatcg actccccgct cgcgcaaaag ctgcatcact ctggggaaca
     1021 ggtagttgtg agccccggag agaatggaca tggccacgac atccacgtcc tcctgaatcg
     1081 ctgccgccac gatctgctcc ggcgtctgcc gaaggcccgt gtatatcact tccatgccag
     1141 cgtcgcgcag ggcgcgcgcg accaccttcg cgccccggtc atgcccgtca aggccgggct
     1201 tggcgacgag gactctgatc ttcctaccct catccatcgt gtcgctctcc cccttcctag
     1261 taggccaccg tgggcttgta ctcgccgaag acgcctcgga ggacgccgca catctctccc
     1321 agcgtcgcat atgccctcac agcgtccaga atgtacggca tcagattgtc ggaccccgaa
     1381 gccgcgtgct caagcgccgc aaggctcgcc tccactgccg cgttgtcgcg ctccgcccga
     1441 agctgggcga gcttcctaca ctgcagatct cccacggccg gatcgacttt gagaagaccg
     1501 ctgggtcgat cctcttctat ctggaaatcg ttgagcccca ctatcgttcg atcgcccgac
     1561 tctatctcca tctggtagcg gtacgcactc tcttcgatct cggcctggat gaacccgcgt
     1621 tctatggccg ccggagcgcc gccgatctcg tcgatcctgc gtatatagtc cgccgccgcc
     1681 cgctcgatct cgtcggtgag cgcctcgatg tagtaagacc ccgcaagagg atcaatagtg
     1741 tcggccacgc cgctttcata cgcgatgatc tgctgggtcc tgagggcgat gcgcaccgag
     1801 tcctgcgatg gaagcgccag cgcctcatcg cgggagttag tgtgaagcga ctgagtgccc
     1861 ccgagcaccg ccgcgagcgc ctgcaaggtt accctgacga tgttgttgtc gggctgctga
     1921 gccgtgaggg tgcacccggc agtctgagtg tgaaaacgaa gctgcatcga cttgggatcc
     1981 ttagctccga acctgtcgcg catgatccgc gcccacaatc tcctggccgc tctgaacttg
     2041 gccacttcct ccagcagatc gttgtgggcg ttgaagaaga acgagagcct gggcccgaag
     2101 tcgtctacat ccaggcctgc gtcgaccgcg gccttgacgt aggcgattcc gtctgccaaa
     2161 gtgaacgcta cttcctggac tgcagtggcg ccggcctccc tgatgtggta gccgctgata
     2221 ctgatggtat tccacctagg cacagcggta gagcagtact cgaagatgtt cgtgatcagc
     2281 ctcattgatg gttttggcgg gaatatatag gtgccccggg cagcgtactc cttgagaatg
     2341 tcattctgaa tagtgccgtt cagcttgttg gcgaagacgc cctgcttttc agcgacggca
     2401 atgtacatgg caagaagcat ggacgcagga gcgttgatcg tcatcgacgt ggagaccttg
     2461 tcgagaggta tcccatcaaa cagcgtctcc atatcggcca aagagtcgat ggccacgccc
     2521 accttcccta cttcgcccgc ggaaagctca tggtcagagt cgtacccgat ctgcgtaggc
     2581 aggtcgaaag cgaccgacag gcctgtctgc ccctgctcca gaaggtactt gtagcgtctg
     2641 ttcgactcct cggctgtcgc aaaccctgca tactgcctca tggtccagaa tcggccgcgg
     2701 tacatcgtgg gctggacgcc gcgggtgtac ggatactcac ctgggaaccc tagatcattg
     2761 acgtaatcta cctgttcgac atcgagcggc gtgtacagcg gcttgacttc tatccccgag
     2821 ctgccttcga atctctcctt gcgctcaggg aatctggcgc aagtcttcgc gtacggaccg
     2881 ctcttccagc gaccatactc gcccgaaaga tccccctggt tcaatgtgat gatccccccc
     2941 tttgaaatag gcagacaacc ttatgggata ttcaacataa acccagaatc acctcttgac
     3001 cacactactc gcggcatcga accgcttaag cctaagcgag ttggatacga ccgacacaga
     3061 acttgcggcc atggctgcgc cggcgtatat cggcggcatt atgccgaacg cagccaaagg
     3121 gatacccacg gcattgtaga tgaacgccca gaataggttc tgcttgatga taccgagagt
     3181 agctcttgac agcctgatcg cgtccggcaa cctgtggagg cccccgacta tcgtgatgtc
     3241 cgcggcctcc atggcgatgt cggtgccggc gctcatggcg atcccaacat ccgccgtggc
     3301 gagagcagga gcgtcattaa tgccgtctcc caccatggcg acgtgcaggc cacgccgccg
     3361 catcttttcc acctgctcag ccttgccgtc aggcaacact tcggccatga cgttctcatc
     3421 gggtattccc acctgacgcg ctatggccgc ggctgtacgg gcattgtcgc cggttatcat
     3481 gtagacctcg aggcccagcc cgaaaagctc cgcaatcaca ccggccgact ctgggcgaag
     3541 ctggtcggcg attccatacg cggccgtaac agtaccgtcc accgcctgca ggactccggt
     3601 ctcgcccttc tcctccatgt gctctagggc tctcactaca tcgtccgcta cgtcgacccc
     3661 gagatcagac aagaatgcga gcgtcccaac cgccacctgg cgcccgtcca cctgtgccgt
     3721 aaccccgcgg ccgggcacac tgcgaaagtc ggtgggcatc ggcgcgttca gaccctgaag
     3781 atccgcctcc tccacaatcg ccctgcccaa tggatgctcc gacgccctct cagcagcagc
     3841 cgccaacctc agtgcttcgc cgggcttcag cgacaggaac accttagaat cgactactcg
     3901 gggcttgccc tgggtaagcg tgcccgtctt gtcgacgact accgcatcga tccggctcag
     3961 cctctccagg tgttcaccgc cgcggatgag cacccccagt tcggccccgc gccccgtggc
     4021 caccattatc cccgtaggcg tagctagccc gagtgcacac gggcatgcga tgaccagcac
     4081 ggacgtggca ttaaccaggg cctgcgcaat gcctacccct gcgaagtgcc aaccgacaaa
     4141 ggtgaccgcc gcaatcgcca gaacagtggg gacgaagtaa gacgctacca cgtccgcgag
     4201 ccgttgtatc ggagccttgc ttccctgggc ttcctccacc agacggacta tctgcgccag
     4261 cgcggtgtct gctcccacgt gagtagcgcg catcttgaac atgccgtgct ggtttaccgt
     4321 tcctcccacc gccgcatcgc cggcgccttt gtccacaggg atactctcac cggtaagcat
     4381 ggattcatcg atggatgtat agccttcgat gatctctccg tccacaggca cccgctcgcc
     4441 gggcctcacg atcactatgt cgccgggcat aacttgttct accgctaccg t
//
LOCUS       DAOTAC010000217         4487 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_15893_length_4487_cov_5.055731, whole genome shotgun sequence.
ACCESSION   DAOTAC010000217 DAOTAC010000000
VERSION     DAOTAC010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4487
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15893_length_4487_cov_5.055731"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(393..633)
                     /locus_tag="PLM74_09075"
                     /pseudo
     CDS             complement(393..633)
                     /locus_tag="PLM74_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006623038.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            complement(630..851)
                     /locus_tag="PLM74_09080"
     CDS             complement(630..851)
                     /locus_tag="PLM74_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017715838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HON43263.1"
                     /translation="MPLYKVIVEKHQDGYVAYPLGLRGVVVGEGNTYEEALDNVKSAI
                     RFHIETFGSDVVETDSAVEVFVAEAKVAT"
     gene            complement(981..2651)
                     /locus_tag="PLM74_09085"
     CDS             complement(981..2651)
                     /locus_tag="PLM74_09085"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HON43264.1"
                     /translation="MDIQDSDGVSDGKQIAWRAWDTQPFDEARAGEKPVLLSISASWC
                     HWCHVMDRTTFSDRDVAALVNEAYVPVRVDTDVRPDVNARYNMGGWPTVAILDHDGHA
                     LAGATYVPPDRMLEWLGRVSRAYPALTRSRTGGDDDTDQHRKRELSYDGEYDDVGTPD
                     REPNLDPYYDVLGDVMASYDPVYGGFGDAPKFPMFDAINLAVFAYRVSADPSYRQIFE
                     HTLHAMTSGGTYDKVEGGLFRYSTVRDWSVPHFEKMLLDNALLVHALANAYQAVQDPG
                     LKAAAHSTVRYMNSVLFQPSIPAWSGSQDADEEYYAIAAAEERARRAPPRVDTRIYVD
                     WNGRASAAMIAAGRVFDEPEWTSRGVQVLRAIIDKCLDARYGLAHLYDGRPHLFGLAH
                     DTISVGAACLHAFEATGDRIWLDLSSSLAQSLIASHAAARHLRGAAGLVSRIPNPDDP
                     PAFNEPARDYRENAYAAIWFRRLARAMSPGDDGSAEVGSVGVCTAPGVCSAPSPPSVS
                     GETSEILRAASSCLAHCRTRYEDHGLMASAYALALGHLALSPICDVCP"
     gene            complement(2831..>4487)
                     /locus_tag="PLM74_09090"
     CDS             complement(2831..>4487)
                     /locus_tag="PLM74_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43265.1"
                     /translation="QRQLLTEKQAALVRCIGEYYSASDTASLRQALSTIMARAVAPAH
                     IEVQAWAGAVNVTGHAQIEIVKSTGEVVQSTRTAQDPPVVRAQVDEGQYTVRGRLVVG
                     SQVLTAQATAIAVKPGQTSLVRLDFGPLDGKVRVTARASGQDVSDRVDIRVLKAGAPV
                     AASWAGIPPATNLPAGDYTVVVTHRQYLELTRTAAATVRAGQDTYVDVDLGELPATLE
                     VTVTYRGAVISNLCQLSVFAPGQATQVIRSTSDGQVFRWTSTPGTYDLSVMYRDLVTV
                     EKGVPGVQLAGGRTTNITVTLDDLLGTLRTHVVASGRDVTADSHVTAAGRDGQIELPY
                     TAGARQALVTPGVYAVTAVYRDSESDMYETYVKPGEITDLTLEVKLPGLIAIVPTIEG
                     KPMRTDKLRAWASLDGVSVGTVAIQPDRVELTVPEGAYTVVGEISDPFAQRREAPKVQ
                     VRAGETVQVKMDFDPAGLLRVKVMSDGKPLTEAKVMVYLDGKGGGMWMNQVDKGVWEM
                     EVAEGTHIISIVPSAKGMKSQGIPGVEVASGAIVEVVFDVSSP"
BASE COUNT          856 a         1390 c         1369 g          872 t
ORIGIN      
        1 cgcaatccaa aacgggcggt tcgcccgtcc gcagctgcaa ccaccgagtc tgccccgcgg
       61 gctgcgtgtt ctcttccctg aactcacgtt ccgcatgtag tacatggaaa gcaggcattt
      121 cggaaatgag cagctaggca agcaacctga gatttgggga atcgcgaagc tacactcaag
      181 gaagactcgc taccgaccgt cgggttttcc ggcaggctgg ataaacccct gctacgcatg
      241 gcgttgacgg gcccatcccc gcgtaaggcc gctttgtcat ccgactggga tgcccagggc
      301 agcgcccaaa tgcggaaatg ggaacatcta catgcggaac gtgggtcgca ggactatcgg
      361 ggacggtcag cacgaaagta tgatcttggg gatcacgatt gtcggtaggc tttcaggaaa
      421 tcctccctgc taatccctga ttgagtacag atgacacgca gagtggaagc tttcaaccta
      481 gggtggttgg gcattgttag cggggtgcag gtaccgtcgg gattgtggcg gatcatcgca
      541 atgtgctcgc actccctgac gagttgaaaa acccaacaat ccgaaagcgc ctactacctt
      601 tgctttcggc gcatcgacag ggaacctcgt catgtcgcaa ctttcgcttc ggcaacgaac
      661 acctcgacag ctgagtcagt ctcgacgaca tcgctcccga aagtctcgat gtggaaacga
      721 attgccgact taacgttgtc tagcgcttcc tcgtacgtgt tgccctcgcc gacaactacc
      781 cccctcaatc ctaaggggta tgctacgtag ccgtcctggt gcttttccac gatgacctta
      841 tagagcggca tttccactcc ccctggtaga tgatcttggc ttcagtatac cacgacgcgg
      901 catgagcgaa aatgggaagc aagtgcgcag tgaccacaca gacattcaac tcggcattgc
      961 ctacagaacc tgcacgcgtc ctacgggcac acgtcacaga tgggcgacag cgcaagatgg
     1021 ccaagggcga gggcgtaggc cgaggccatc aggccatgat cctcatagcg ggttcggcaa
     1081 tgggcaaggc atgagcttgc ggctctcaga atctcggagg tttcacctga cacactgggt
     1141 gggctgggcg cgctgcagac gccgggggcg gtgcacacgc caaccgagcc gacctcagcg
     1201 gagccatcat cgccggggct cattgcgcgg gccagccggc gaaaccagat ggcagcatag
     1261 gcgttttccc ggtagtcgcg ggcaggttcg ttgaaagcag gcgggtcatc ggggttcgga
     1321 atcctggaaa ccagccccgc cgctccccga agatgccgcg ctgcggcgtg gcttgcgatc
     1381 agcgactggg ccagtgaaga cgagagatca agccagatcc ggtcgccggt cgcctcgaat
     1441 gcatgcaggc aggcagcgcc caccgaaatc gtgtcgtggg ccagcccgaa cagatggggc
     1501 ctgccgtcat acaggtgggc taggccataa cgtgcgtcga ggcacttgtc gatgatagca
     1561 cgtagcacct ggactccgcg cgaggtccac tcaggttcgt cgaacacgcg cccggcggcg
     1621 atcatagctg ccgacgcgcg cccgttccag tcgacgtata tgcgggtatc gactcgcggc
     1681 ggggcgcggc gggcgcgctc ttctgcggcg gctatcgcgt agtactcctc gtctgcgtcc
     1741 tgcgagcccg accacgccgg aatgctcggc tgaaacaata cagagttcat gtagcgaacg
     1801 gtgctatgag cggcggcttt gagtcccggg tcttgtacgg cctgatacgc gtttgccagt
     1861 gcatggacaa gcagcgcgtt atccagcagc atcttctcga aatggggcac gctccagtct
     1921 cggaccgttg aatatcgaaa gagaccgccc tccaccttgt cgtaggttcc gcctgaggtc
     1981 atcgcgtgaa gggtgtgctc gaagatctgt ctgtacgagg ggtcagcact gacgcggtat
     2041 gcgaaaacgg caaggttgat cgcgtcgaac atcgggaact tgggggcgtc tccgaacccg
     2101 ccatacacag gatcgtagga agccatcaca tcgcccagca cgtcgtaata ggggtccagg
     2161 ttgggctcgc ggtcgggagt gccgacgtca tcatattcgc cgtcgtagct gagctcgcgt
     2221 ttgcgatgct ggtctgtgtc atcatccccg cccgtgcgcg aacgggtgag ggcggggtag
     2281 gctcgcgata cccgacctag ccactccagc atcctatccg gaggtacgta cgtcgcgccg
     2341 gccagcgcgt gtccgtcatg atcgagaatg gccaccgtcg gccatccgcc catgttgtat
     2401 cgggcattca catcggggcg tacgtcggtg tcgacgcgta cggggacgta tgcctcgttg
     2461 acgagagcag cgacatcgcg gtcggagaag gtcgttcggt ccatgacatg acaccagtgg
     2521 caccacgacg ccgatatcga cagaagcact ggcttctccc cggcgcgtgc ttcatcgaat
     2581 ggctgggtgt cccaagctcg ccatgctatc tgcttcccat ccgaaacgcc gtcactatcc
     2641 tgaatgtcca cgtgcaccgg tgcctccttc tccgcgtgtg atagctgatc gtccgccgag
     2701 cggcgagcat ccatatgagt ataccccaag acggaccgtg aaaagcgaag ggcgaggctg
     2761 ccagcctcgc ccctcagtat acctgcgctg agcaatccgg cgctcgagcg ctgtcatgct
     2821 gaacctaacg tcaaggagaa ctgacgtcga agacgacctc aacgatagcg ccgctggcga
     2881 cttctactcc gggaatcccc tgcgatttca tgcccttagc gcttggaaca atggagatga
     2941 tgtgggtgcc ttcggcgacc tccatctccc atacgccctt gtctacctga ttcatccaca
     3001 tcccgccacc cttgccgtct aggtacacca tcactttggc ttcggtgagg ggctttccat
     3061 cggacataac cttgacgcgt aggagtcccg caggatcgaa gtccatcttg acctgaaccg
     3121 tttcgcctgc ccgcacctgt actttgggtg cctccctgcg ctgggcgaac ggatcggata
     3181 tctcgcccac aacggtgtag gcgccttccg gcacagtcaa ctcgactcta tcgggctgaa
     3241 tcgcgacagt gccgactgac actccgtcaa gactcgccca tgcacggagc ttgtcggtcc
     3301 tcatgggctt gccctcgatt gtggggacta tcgcgatcaa gccgggtaac ttgacctcga
     3361 gggtcagatc cgtgatctcc ccgggcttga cgtaggtttc atacatatcg ctttcggagt
     3421 cgcggtagac cgcggtcacg gcgtagacgc ccggtgtcac aagcgcctgg cgcgcgcccg
     3481 ctgtgtaggg cagttcgatc tggccgtcgc ggcccgccgc cgtcacgtgc gaatcggcgg
     3541 taacatcgcg cccgctggct actacgtgcg tccggagcgt gccgaggagg tcgtccaagg
     3601 tcactgtgat attggtcgtt cgaccgcctg ctagctgcac gccgggaacg cccttctcaa
     3661 ctgtgacaag atcgcggtac atcacactga ggtcataggt tccgggagtt gaggtccaac
     3721 ggaagacttg tccatcagat gtgctgcgaa tcacctgcgt tgcctgccca ggcgcgaata
     3781 cgctcagctg acacaggttg gatatgaccg ctccgcggta ggtcacggtc acctcgagcg
     3841 ttgccggcag ctcgcccagg tccacgtcga cataggtatc ctggccggcc cgaactgtgg
     3901 ctgccgccgt gcgggtgagc tcaagatact gcctgtgggt gacgaccact gtgtagtcgc
     3961 ccgccggcag attcgtggcg ggcggaatcc cggcccaact cgcggcgacg ggcgcgcccg
     4021 ccttcagcac gcggatatcg acgcggtcgc taacgtcctg ccccgacgcg cgggcggtca
     4081 cgcgaacctt gccgtccagc gggccgaagt ccagcctcac caagctggtc tgcccgggtt
     4141 tcacggcgat cgccgtggcc tgagccgtca gaacctgtga gcccacgacg agacgaccgc
     4201 gcactgtgta ctggccctca tctacttgag cgcgcacgac aggggggtct tgcgcggtgc
     4261 gggtcgactg cacgacctcg ccggtggact tcacgatctc gatttgcgcg tgccctgtca
     4321 cgttgaccgc gcccgcccag gcctgcacct ctatgtgcgc cggggcgact gcgcgcgcca
     4381 tgatcgtgga gagagcttgc ctgagcgatg ctgtatccga agccgagtag tactcgccaa
     4441 tgcagcgcac cagagcggcc tgcttctcgg tgaggagctg gcgctgg
//
LOCUS       DAOTAC010000218         4463 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_15976_length_4463_cov_4.471642, whole genome shotgun sequence.
ACCESSION   DAOTAC010000218 DAOTAC010000000
VERSION     DAOTAC010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4463
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15976_length_4463_cov_4.471642"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="PLM74_09095"
     CDS             complement(<1..562)
                     /locus_tag="PLM74_09095"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HON43266.1"
                     /translation="MSSILAAGLIMLSGVALGRVAAGLGVSSVVGYLLAGITLGYAWP
                     GIGSAITPAGAAFAAEMGLALIAFVVGGELEARRVRSLPRGAVSIAFAQASLCMAAVL
                     AAAVITGEPPALGLALGAIAAATAPSTTIMIIRRHKADGPLTRMLLSVVAVDDAVCIM
                     LYAVAVSVARALPIAGAPQSVLGVVGV"
     gene            complement(590..961)
                     /locus_tag="PLM74_09100"
     CDS             complement(590..961)
                     /locus_tag="PLM74_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43267.1"
                     /translation="MQVLFVVLNDTALLDDLFSSFLRAGITGATCIESMGMGRALSEI
                     DSSSIPIFAALRHHLNESRPYNRTVFALLPDEKVDEAIAAVESVVGDLSRPGSGLLFT
                     VPVGKIAGFPKRHSNLDDLGQ"
     gene            1146..1739
                     /locus_tag="PLM74_09105"
     CDS             1146..1739
                     /locus_tag="PLM74_09105"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HON43268.1"
                     /translation="MVSSLIDITRPVYHGMPVWPGDPDVRFVPTLTIPTCGANVTSLG
                     MSTHSGTHIDAPSHYLAGAPTVDRVPLESLVGPCLVIEPAAKGPIGRRSLMESMETPH
                     PPRVLMKVPTGAYLDAGGAAYLVEQGIVLIGTEEMSIESPAGDGSVHTRLLSAGVVIL
                     EGADLSLVPSGQYHLTALPLRLIGLDGAPCRAVLIPL"
     gene            1972..2169
                     /locus_tag="PLM74_09110"
                     /pseudo
     CDS             1972..2169
                     /locus_tag="PLM74_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017415000.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
     gene            complement(2368..3189)
                     /locus_tag="PLM74_09115"
     CDS             complement(2368..3189)
                     /locus_tag="PLM74_09115"
                     /inference="COORDINATES: protein motif:HMM:NF013397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HON43269.1"
                     /translation="MDPVAYRTPSEIAATAVNAGVAKSRASAVTLLVLGFLAGSFIAM
                     AAIASHTVSYALGAWAGPSAVKLISGLVFSVGIVMVIIGGAELFTGNSLMVMALLDRR
                     IRLGALIRNWVIVYFSNMAGAVFVAWLYHESGLWQPVDGASAQALVMTAMRKSSLLFW
                     PAFFRGVLCNWLVCMGVWMSYSADDVVGRIAPLAMAVSAFIMCGAEHSVANMFYIPIG
                     LMLSSAPAAEYVASLARNLLPVTLGNVVGGGLLVGAMYWWAYMRPVSEPRGIAGR"
     gene            3376..4200
                     /locus_tag="PLM74_09120"
     CDS             3376..4200
                     /locus_tag="PLM74_09120"
                     /inference="COORDINATES: protein motif:HMM:NF019128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HON43270.1"
                     /translation="MRSRLHTNSFKSLAAFAAAALIVAMAACVASAATTYTVKTGDSL
                     YLIAQRFGVTVAALRAANGLSHDWIYPGERLTIPSSGGTPAPSGSTYTVRSGDSLFLI
                     ANRFGVTVSALRSANGLSYDWIYPGEVLRIPAGGSSRPPETPSRGDYGSWSSTDLSLL
                     ARLVTAESGGEPYEGQVAVAATVLNRVKSGSYPNTISGVIYQVVDGRYYQFSPVLDGR
                     INTAPSSTALRAVQDAVSGWDPSYGALGFYNPSKTSNYWVQLRPVTRAIGGHVFFK"
BASE COUNT         1000 a         1391 c         1208 g          864 t
ORIGIN      
        1 agacgcccac tacgcccaga acagactgcg gcgcgcctgc gataggcaga gcgcgcgcca
       61 cagacacggc aacggcgtac agcatgatac atacagcatc atccaccgcc actacagaca
      121 agagcatccg ggtaagcggc ccgtccgcct tgtgcctcct gattatcatg attgtggtag
      181 atggggctgt ggctgcggca atcgcaccca atgcgaggcc aagggcagga ggttcgccgg
      241 ttatgaccgc cgcggccagc actgccgcca tgcacaggct cgcctgtgca aatgcgatgc
      301 tgacggctcc tctgggcagc gagcggaccc tccgggcctc cagctcccct cccacgacga
      361 aagctatcag cgccagaccc atctctgcag cgaacgccgc gcccgccggg gtaatagcgc
      421 tgccaatgcc gggccacgca tagcccagcg taatccctgc gagaaggtat cccacaacgg
      481 acgaaacgcc taagcctgcc gcaacccgcc ccagggcgac accgctcaac atgataagac
      541 ccgccgcaag aatgctgctc atgtaaaacc tctccgccaa cacgcgaggt tactgaccga
      601 ggtcgtccaa gttagagtgg cgtttgggaa accccgcgat cttgcctacc ggaaccgtga
      661 agagtaatcc cgagcccgga cggctcaggt cgccaacgac gctctcaaca gccgctatgg
      721 cttcgtcaac tttctcgtct ggaagcagcg cgaacaccgt tctattatac ggcctgcttt
      781 cgttcagatg atgtcgcagc gctgcaaaga tcggtataga ggagctgtcg atctcagaca
      841 gggcgcgccc catccccata ctctctatgc acgtcgcccc ggttatccct gctcgaagaa
      901 aagatgagaa caggtcatcg aggagcgcag tatcgtttag caccacaaac aggacttgca
      961 cacgaattcc ctccagaaga ggcaagtgtt gcgttgttac tatttactac tatacctaac
     1021 tgctcagttc actgcaatac gctgccacgc catccgtgtc gaccttccat atccgtacag
     1081 cgggcgattg cggcaggata atgattgacg cccgagaaaa gtactgacct gggatggtga
     1141 tgcacatggt cagctcgctc atagacatta ctcgccctgt gtaccacggg atgccggtgt
     1201 ggccgggaga tcccgacgtt cgattcgtgc ctacgctaac gattcccaca tgtggcgcca
     1261 acgtcacatc gcttggcatg agtactcact caggcacaca tatcgatgct ccttctcatt
     1321 atctagcagg tgccccaaca gtggatcgag tgccactgga atcactggtc ggcccttgcc
     1381 tagtcatcga acccgccgcc aaaggcccca ttggacgccg aagcctgatg gaatccatgg
     1441 aaactcctca cccacctcga gttcttatga aggtccccac aggcgcgtat ctcgacgcag
     1501 gcggcgccgc ttacctcgtc gagcagggta tagtactgat aggaactgaa gagatgtcca
     1561 tcgaaagccc ggctggagac ggcagtgtcc acacccgcct tctttcggca ggtgtagtga
     1621 tactggaagg cgcagatctg agtctcgttc catcgggtca gtaccatctg accgcactgc
     1681 cgctgcgcct catcgggctt gatggagcac cgtgccgggc agtcctaatc ccactttgac
     1741 tgcattgttc catactatcc cacttatggg tccaccagcc ttacttcggc tgagtgcgcc
     1801 tgcaattctt cgagtgtgtg cggcgtcacc tgtcttttct ccgaccggtc cagttttcct
     1861 tcgtaccata tagtgattcc ttgggtgact caaagttcta gtatatgccc acatgcagcg
     1921 gtcagactac gaatgcaacc gcgacacctg caagaaagga ggaggcatcg catgccgaga
     1981 aggagcaaca ggatcttgaa ccctcaggcg cgtcaagcgc tgaatcagat gaagtatgag
     2041 attgcgagcc aggtgggcgt ggactacaac acctatggcg gataccttgg cgatatcccc
     2101 gcgcgtgagg cgggcagaat cggcggccag atggtcaagc agatgatcgc cgcggccgag
     2161 cagagcttga taagccagac agtcgccgga acccaggcca gtttcgcgca ggccctcagg
     2221 ccttccgggt tcacgtctga aaccgcatcc ggttttaatg tcccgaccaa cctgaacgtt
     2281 cccaagtcgt agcctgcaac acaaggtacg cagggcgcga tagcgcggct atcgcccggt
     2341 cgcccctcga ccgggcgctg gccgcggtca tctgcccgcg attccgcgtg gctccgacac
     2401 agggcgcatg tatgcccacc agtacattgc ccccactagc agacctccac caaccacatt
     2461 gcccagagtc accggcaaca ggttccgggc gagactagcg acgtattcgg cagctggagc
     2521 tgaagacagc atcagaccga taggaatgta gaacatgtta gccactgagt gttccgctcc
     2581 acacatgatg aaggcagaca ctgccatggc cagcggcgct atcctgccga caacgtcatc
     2641 ggcggaatac gacatccata ctcccataca taccagccag ttgcacagca ccccacggaa
     2701 gaaggccggc cagaacagaa gcgacgattt tcgcatggca gtcatgacga gcgcctgggc
     2761 ggaggcgccg tctaccggct gccacagtcc ggactcgtga tatagccacg ctacgaagac
     2821 agcgccggcc atgttggaga aatagacaat gacccagttg cggatgagcg ctcctaatcg
     2881 aatccgtctg tcgaggagcg ccattaccat caaggagttc ccagtgaaca gctcagctcc
     2941 tcctattatc accatgacaa tgccgacact gaacactagg ccagagatga gtttcaccgc
     3001 actcggccct gcccacgcgc cgagcgcgta tgacactgtg tgggatgcta tagccgccat
     3061 cgcaatgaag cttcctgcaa gaaaacccag tacaagaagg gtaaccgcac tggctcggga
     3121 tttggccact cccgcattga cggcggtcgc ggcgatctcg gaaggcgtcc tgtacgccac
     3181 tggatccacc actctttctc acgtcgttcg ctatactcca actatctcaa gggcttcaag
     3241 ctctgtcaag catcggcctc ggaggtgctt tcccaccact tcccacaggg aattccacat
     3301 gtagttgtag aatagacagg ccgagagggg gcccacactt ccgcgcaggc ttaacagaga
     3361 aaggagttgc aattattgag atctagacta cacactaaca gtttcaagtc cttggcggca
     3421 ttcgcggcgg cggccctgat tgtagccatg gccgcatgcg tagcgtcagc cgccacaacc
     3481 tacaccgtca agacaggcga ctcactctac ctcatcgccc aacgcttcgg cgtgacggta
     3541 gctgcgctgc gcgccgcgaa cgggttgtca cacgattgga tataccccgg tgaaagactg
     3601 acgatcccct cttcgggagg cacaccggcg ccgtcaggct ctacctacac agtaagatca
     3661 ggtgattcgt tgttcctcat cgcgaaccgc ttcggcgtta cagtaagcgc gttgcgctcc
     3721 gcgaacgggt tgtcatatga ctggatctac ccgggtgaag tcctgcgtat cccggcaggc
     3781 ggtagttctc gtccacccga aaccccgtca cgaggggact atggctcttg gagctcgacc
     3841 gacctctcgc tccttgcccg actggttact gccgaatccg gcggtgagcc gtatgaaggg
     3901 caggtcgcag tggcggcgac cgtgttgaac agggtgaaaa gtgggtcgta tcccaatacg
     3961 atctccggcg tgatctacca ggttgtagac ggcagatact atcagttcag tccggttctc
     4021 gacggtagga tcaataccgc tccctcctct acagcactga gagcagttca ggatgcggtg
     4081 agcggatggg acccatccta cggcgcacta gggttctaca atccctctaa gacctccaac
     4141 tactgggtac agctccgccc tgtcactaga gcgatcggag gtcacgtctt cttcaagtag
     4201 agcgagcgcg ccagagcgct ggaagacagg cccgaccact ccccgaggcg cattcgcctc
     4261 ggggttcgcc ttgttccgac caatcaacgt caagccgtct attgagttca gcgccatatg
     4321 gttgtaaaat tgaccgaggg agggactagc agtgccaatc tatgagtaca actgcagcaa
     4381 ctgcggcaaa tttgaatttc tacacggacc aggcgaaggc gcgctcacgc gctgccccaa
     4441 gtgcggttct cctgtccggc gcc
//
LOCUS       DAOTAC010000219         4447 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_16048_length_4447_cov_4.407332, whole genome shotgun sequence.
ACCESSION   DAOTAC010000219 DAOTAC010000000
VERSION     DAOTAC010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4447
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16048_length_4447_cov_4.407332"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..1582
                     /locus_tag="PLM74_09125"
     CDS             8..1582
                     /locus_tag="PLM74_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013656983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HON43271.1"
                     /translation="MEFGWWTIVPPLVAITLALVTKKVILSLGIGILSGALIASHFSI
                     VGMFTVAATTLWEKVTDMWNVSILIFLVCLGILTYLVTIAGGARAYGDWATKRIKTRA
                     GAQLASLLLGILIFIDDYFNCLTVGTVMIPVTDRHRVSRAKLAYIIDATAAPVCVIAP
                     VSSWVVTIMSTMGDKFRAVGIEMEPFVAFLRTLPLNLYAWLTLGMVAVVAILELDFGP
                     MEKFEHEARATGNVNAAKPAGAAKRQPAISPKGTVWDLLVPVIGLIIFAIIMMAYTGG
                     YWSGEGMSFLDAIKNTDSATALMYAGFLGLALTLAVVLPRGVVSVSRIPEAIWGGFLA
                     MLPAIIILIFAWTIGGVIGQLGTGEFLAHQVGVHLNPAWYPVIMFLLACLISFSTGTS
                     WGTFAIMVPIAIDFAVLLAPEIMLGMIGAALAGAVYGDHCSPISDTTILSSTGAQCNH
                     IDHVSTQLPYATVAAVAVAIGYVFYGFLARTGASAGLTAVLTLLVAAAVLYVIIRVLH
                     TRSIRKEAASGRAVGK"
     gene            1839..2732
                     /locus_tag="PLM74_09130"
     CDS             1839..2732
                     /locus_tag="PLM74_09130"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HON43272.1"
                     /translation="MARFGIHLSISGGVAQAAARARELGCDAFQIFSRNPRTLKAKPI
                     DPDDAAAFRTAVQESGIHPVVVHVNYLINVASPKDDTYRLSVDALTDELIRADILGAE
                     LVVMHPGNHLGSGAQAGAERIASAIDRAYESTGARARLCLENMAGAGTEIGTTFAELR
                     MIMDLAQCGPTLGVCFDTCHAYGAGYDVSTARGLRSVIDEAARTVGIDRIVVVHANDS
                     KGQLGGRKDRHEHIGDGAIGLAGFRNILACAELADLPFILETPVDSPEDQVRDLATIR
                     SCAPPPQDEPRQLTGRMRVEV"
     gene            2722..>4447
                     /locus_tag="PLM74_09135"
     CDS             2722..>4447
                     /locus_tag="PLM74_09135"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HON43273.1"
                     /translation="MRSEPVGAVANYISPEAADALRREIEQIDGAEVFAVGRCDPNYV
                     VCDLSVLARGNRGAAPVVDPTLIRGQVVIHNHPSGDLTPSDADVTAAASLAARGIGFW
                     IVDNSVSRIRSVTDPLTNDMGSIIIDPADIRHLFSPAGPFAARFPGYETREGQIEMAL
                     AVAQAFSDGGHLVVEAGTGTGKSLAYLAPAFLWARRNSTRVVVSTNTINLQEQMVHKD
                     IPAISEALGEEVRAVLVKGRANYLCLRKLNRVLADQDRAFEPALRGAFGQVLEWAYGT
                     DTGDRSDMGFEPNPDVWEMVSSESDMCLHARCPHFGECFFHRARKEMDSAEILVVNHH
                     ILFADASIRSQLGPDAERAVLPRYRAVILDEAHNAADVATEYFGRMGSRTGLIRLINA
                     VYRREGRPGGTGGSWGAGASGAHRGDSGQADYGALVRLRGALYSDWADTSRQSQLRAA
                     LDLIDMEAASSAIRLREVGETFFEEVRQLALRSAGDGGERTLRITEEIRSGPEWDRVI
                     WPAHERFAAALEDFAECLVRLVNILRGNAESTGDADSTGDADNTSDSESSGGTDVYAE
                     TVELAAYAN"
BASE COUNT          841 a         1298 c         1407 g          901 t
ORIGIN      
        1 gataggcatg gagtttggtt ggtggaccat tgtgccgcca cttgtggcaa tcacactggc
       61 gctcgttaca aagaaggtca tactgtcact gggaatcgga atcctttcgg gcgccctcat
      121 cgcctcgcac ttcagcatcg tcggcatgtt cactgtcgcg gcaacgacct tatgggagaa
      181 ggtcactgac atgtggaacg tgtcgatcct gatcttcctg gtatgcttgg gtattcttac
      241 gtatctggtg actatcgccg gcggcgccag agcgtacgga gactgggcaa ccaaacggat
      301 caagactcgt gcaggcgcgc agcttgcaag ccttctgctg gggatcttga tcttcatcga
      361 cgactacttc aactgcctta ccgtgggcac cgtgatgatt ccggtgaccg accggcatcg
      421 tgtgtcacgc gcgaagcttg cctacattat tgacgcaacc gctgccccgg tctgcgtgat
      481 tgctccggtg tcgagctggg ttgtgaccat aatgtcaaca atgggcgaca agttcagggc
      541 agtcggcatc gagatggaac cgttcgtcgc gttcctgcga acgcttcccc taaacctcta
      601 tgcctggctg actctgggca tggtagcggt tgtcgcaatt ctcgagcttg acttcgggcc
      661 tatggagaag tttgaacatg aagctagggc cacggggaac gtcaacgcgg ccaagccagc
      721 cggcgccgca aagcgccaac cagcgatcag ccccaagggt actgtatggg accttcttgt
      781 ccccgtcata gggctgatca tttttgccat catcatgatg gcgtacaccg gtggttactg
      841 gtccggggag gggatgtctt tcctcgatgc catcaagaac accgattccg ctacggcgct
      901 gatgtacgct ggtttcctgg gccttgcgct tactcttgcc gttgtgttgc ccaggggagt
      961 ggtatcggtc agccgaatac ctgaggccat atggggcggg ttcctggcga tgctgcccgc
     1021 catcataatc ctcatattcg cctggacaat aggcggcgtc atcggtcagc tgggcacagg
     1081 cgaattcctg gcacaccagg tcggagtaca ccttaatccg gcgtggtacc cggtgataat
     1141 gttcctgctc gcctgcctaa tctcgttctc taccggcact agctggggca cgtttgcgat
     1201 catggtcccg atcgcaatcg acttcgcggt gctcttggcg cctgagatca tgctgggcat
     1261 gattggggca gcgctggcgg gcgcggtata cggcgaccac tgctcgccaa tatccgacac
     1321 tacgattctc agctccacgg gcgctcagtg caaccacatc gaccatgtgt ccacgcaatt
     1381 gccatatgcg acagtggctg cggttgcagt tgccatcggc tacgtctttt acggcttcct
     1441 tgcgcgtacc ggggcctcag cggggctgac ggcagtcctg acactgctgg tggcagcagc
     1501 tgtgctgtat gtgataatca gagtgctgca cacccggtcc atcaggaagg aggccgcatc
     1561 aggcagagct gtgggtaagt gacgatcggc gcgggcgtct gcggcatctg agcgatatgg
     1621 cttcagcgga cggggcgggg cggggtgggg tccccgcccg ttcggctgta caccggcggt
     1681 tcgttcccat gtcctgtggg ccagatgcaa gagacccgaa tcatgtcgac atcgcgctgg
     1741 acgatgccct cgatgacgca aggtatccag gcggttgttt cggcttgtga ctgtgctgcc
     1801 caaacggtag aattagaaat ggcaaagggg gctccgacat ggcacgattc ggaatacatc
     1861 tgtcgatatc aggcggggtt gcgcaggcgg cggcgcgcgc tcgcgagctc ggttgcgacg
     1921 cattccaaat attcagccgt aaccctcgca cgctcaaggc caaacccatc gatcccgatg
     1981 atgcggccgc gttccgcacg gccgtgcagg agtcgggcat acacccggtc gtggtgcatg
     2041 tgaactacct gatcaacgtg gccagcccca aggatgatac gtatcggctc tcggttgacg
     2101 cgctcacaga cgagcttatc cgtgccgaca ttttgggagc ggagctggta gtgatgcatc
     2161 ctggtaacca tctcgggagt ggcgcccagg ctggagctga gcggattgct tccgccatcg
     2221 accgcgccta cgagtccaca ggcgcccgag cgcgcttgtg ccttgagaac atggcgggtg
     2281 ccggaaccga gatcggcact accttcgccg agttgcgcat gatcatggat ctggcacaat
     2341 gcgggccgac gcttggcgta tgcttcgata cgtgccatgc ctacggagcg ggctatgacg
     2401 tgtcgacagc ccgtggactc cggtcagtga tcgatgaggc agcgcgcact gttggcatcg
     2461 accgcatagt ggtggtccat gccaacgact ccaaagggca actgggcggt cgaaaagacc
     2521 ggcacgagca cataggggat ggagccatcg ggctggcggg tttccggaac atcctcgcat
     2581 gcgcggagct ggccgatctt cctttcatac tcgagacgcc tgtggattcc ccggaagacc
     2641 aggtgcgaga ccttgccact atcaggtcgt gcgcacctcc accgcaggat gagcctcggc
     2701 agctgacagg gaggatgcga gttgaggtct gagccggttg gggcggtagc caactacatc
     2761 tcgcctgaag ccgcagatgc gcttcgacgc gagattgagc agatcgacgg tgcggaagtg
     2821 ttcgccgtgg ggcgatgcga tcccaactac gtggtgtgcg atctatctgt gctggctcga
     2881 gggaatagag gcgccgcccc ggtggtggat cctaccttga tacgcgggca ggtggtcata
     2941 cataaccacc cttccggcga cctcacacct tcggacgccg atgtgacggc agcggccagc
     3001 cttgccgcac gtggcattgg cttctggatc gtcgacaact cagtctcccg tattcgttca
     3061 gtcactgacc ctctgaccaa tgatatgggg tcaatcatca tcgatcctgc tgatatcagg
     3121 catctgttct ctcccgccgg gccattcgcg gcgcgattcc cagggtatga gacaagagaa
     3181 gggcagatag agatggccct cgccgtcgca caggcgtttt ccgacggcgg tcacttggtg
     3241 gtcgaggccg ggacgggcac cggtaagtcc ctagcgtacc tggcgccggc cttcctgtgg
     3301 gcaaggcgca acagcacacg ggtagtcgtg tccaccaaca ccatcaacct gcaggagcag
     3361 atggtccaca aagacattcc tgctatctcc gaggcactgg gtgaagaagt ccgcgcggta
     3421 ctggtcaaag gcagggccaa ctacctatgc ctgcgcaaac tcaaccgggt gcttgccgac
     3481 caggacaggg cgttcgaacc cgctctacgc ggtgcattcg gtcaggtgct ggagtgggcc
     3541 tacggaaccg acacgggcga ccgctccgat atgggctttg aacccaaccc ggacgtgtgg
     3601 gagatggtca gctcagaatc ggacatgtgt cttcatgcca ggtgccctca tttcggcgaa
     3661 tgcttcttcc atcgggcgag aaaggaaatg gatagcgccg aaatcttggt ggtcaaccac
     3721 cacatcttgt tcgcagacgc atcgatccgg agtcaactcg gtccggacgc cgagcgcgct
     3781 gtcttgcccc gataccgtgc ggtgatcctg gacgaagccc ataacgccgc cgatgtcgcc
     3841 acagagtact tcggtcggat gggctcccgg accggcctga tccggctgat caacgcagtc
     3901 tatcgcaggg agggcaggcc cgggggcact ggggggtctt ggggcgccgg ggcgtccggg
     3961 gcgcatcggg gcgactcggg tcaggctgac tacggggccc ttgtgcgcct gcgcggcgcg
     4021 ctctacagcg actgggccga cacgtcgcgg cagagccagc ttcgcgccgc ccttgacctc
     4081 atcgatatgg aggccgcctc gtccgccatc aggctccgcg aggtgggtga gaccttcttc
     4141 gaggaagtaa ggcaattagc gctgcgttcg gccggcgatg gtggcgagcg cacattgcgc
     4201 ataacagagg agatacggtc cgggccggag tgggaccggg tgatttggcc cgctcatgaa
     4261 cgattcgctg ctgcactcga ggattttgct gagtgcctcg tacgtctagt gaacatccta
     4321 cgcggcaacg ccgagagcac gggtgatgcc gatagcacgg gcgacgccga taacacgagc
     4381 gattccgaga gcagcggcgg cacggacgtc tatgccgaaa ccgtcgaact tgcggcctac
     4441 gccaacc
//
LOCUS       DAOTAC010000220         4433 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_16112_length_4433_cov_4.868661, whole genome shotgun sequence.
ACCESSION   DAOTAC010000220 DAOTAC010000000
VERSION     DAOTAC010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16112_length_4433_cov_4.868661"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(496..2289)
                     /locus_tag="PLM74_09140"
     CDS             complement(496..2289)
                     /locus_tag="PLM74_09140"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HON43274.1"
                     /translation="MLKHALLHLPYDPMAYPVGEDEIEVVFRAARADLKSVSVVYVDR
                     YGIGLQPKETGLRRVGSDSMFDYWSGRMRLRYGRFMYHFVLSDGQSTMYYGEGGLSAE
                     PVTPEVCNPWAWCFHYPYIWRHYGADDPPAWAADAVVYQIFVDRFANGDEASDPARVD
                     PWGARPTSCSFSGGDLAGIIGRLDYLASLGVSCIYLTPVFLAPSNHKYDTTDYYKLDP
                     HFGDEATARRLVDESHARGIRVMLDAVFNHCGFNFGPFQDVVARGAESPYYKWFNIHD
                     LPVRVHPTPNYETFAMDVPDMPKLRTDNPEVRNYLISSAEYWTRSLDIDGWRLDVADD
                     VDPGLWREFRARIRAIKPEALIVGEILHDATPFLRGDQMDSVMNYPWRELCLHYFADR
                     TIGTDSFADDLTTLHVRYRRQVQYAIWNLLGSHDRPRFLTLCGGDVRRLLLASTFQFT
                     YPGTPYIYYGDEIGMEGDDDPDCRRCMPWNRQEWNEQLLGHYMGLARLRRRHSALRRG
                     EFTVLMADTAESPLVYARWNSRGSARSCMVIALNNSDLPATIDWDLVAARMRQTGVLA
                     RWADRPMRTVWTVGAGDRSELAPMSARVWKV"
     gene            2501..3070
                     /locus_tag="PLM74_09145"
                     /pseudo
     CDS             2501..3070
                     /locus_tag="PLM74_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779959.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
     gene            complement(3529..>4433)
                     /locus_tag="PLM74_09150"
     CDS             complement(3529..>4433)
                     /locus_tag="PLM74_09150"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HON43275.1"
                     /translation="AQFPLARSMYYGIFHSVSAFCNAGFDLFGTVTGPFTSLTGWQSD
                     PVIVLTIGSLIITGGLGFPVIVELIGHHRGNRLSLHTRLVLQVTGILLLGGTLAVLGL
                     ELSNPHSLKSLTWAGKLLGAIFQSITPRTAGFNTLDIGNLRDGTLFVMIILMFIGASP
                     SSTGGGIKTTTFGVLIASVISTARGRDDVEMHERRLPHDEILKAMTIATLAMGLVTGV
                     TLILSVTENARFLEVLFETASAFGTVGLTMGITPNLSDIGRALIMATMFMGRVGPLTV
                     VIALAQRKRKPSTVHLAEERILIG"
BASE COUNT          835 a         1339 c         1363 g          896 t
ORIGIN      
        1 gtaactatcg tttccgagcc taagcaggtc gccggcccgg tagtcagtca gcttctgcgg
       61 tctgggtacc catggcgcgg cgtcagcggt ttcccgctca gctgcaatat gtggatatcc
      121 ggggcgcttc ggcctttgta cccgggccaa tccatggata tgggtaacgt gtagagctaa
      181 agggcctttt ccgcgagtac gcccccttcc atcgtcacta cgtgtggcaa tgtgtgcccg
      241 cgcttcgact tcgcggcact tacagggaat actcagatcc aggagatcgc cccgctgttc
      301 ctcggctggc cgcgctgcct gtcagcctga gccacatcgg tccgacgatg gcggatcgac
      361 aagcattgcg ccgatcgccg gctcgctgac tgctaggtgg aagctcgatg ctggtattgc
      421 aggcgtctct cgtggcggcc ccgatctctc gtggctgcta ccatctctcg cagcgaccac
      481 tgccgctcgc caccgctaca ccttccacac ccgagcgctc atgggcgcga gttccgaacg
      541 gtcgccggct cctacggtcc acactgtcct catgggccgg tcggcccagc gcgcgagcac
      601 acctgtctgc cgcattcttg cggctacaag atcccaatcg atggtggctg gcaggtcact
      661 gttgttcaga gcaatgacca tgcatgagcg ggcactgccc cggctattcc atctggcgta
      721 cactagcggg ctttccgcgg tatcggccat aaggacggtg aactcgccgc gtcggagggc
      781 gctatgtcgc cgtcggaggc gggctaggcc catatagtgg cctaagagct gttcgttcca
      841 ctcctgcctg ttccatggca tgcatcgcct gcagtcggga tcgtcatcgc cttccatccc
      901 gatctcgtct ccataataga tgtagggagt gccggggtag gtgaactgga atgtagaggc
      961 caagagcagg cgcctcacgt caccaccgca tagggtcaga aaccttgggc ggtcgtgcga
     1021 gcccagcagg ttccaaatgg cgtactgcac ctgcctcctg tacctcacgt gcagagtcgt
     1081 caggtcgtct gcgaagctat cggttccgat ggtgcggtct gcgaagtagt ggaggcatag
     1141 ctcccgccag gggtagttca tgactgagtc catctggtcg cctcgcagga acggtgtcgc
     1201 atcatgcagt atctcgccca cgatcaaggc ctcgggcttg attgcgcgga ttcgtgcgcg
     1261 gaactcacgc catagcccgg ggtcgacgtc gtcggcaacg tcgaggcgcc aaccgtctat
     1321 gtcgagcgag cgcgtccagt attccgccga gctgatcagg tagttgcgca cctcggggtt
     1381 atctgtgcgc aacttgggca tgtcgggcac atccatggcg aatgtctcat aattcggcgt
     1441 ggggtgcact cgcaccggca gatcatggat gttgaaccac ttgtagtagg gcgattcggc
     1501 gccgcgggcc actacgtcct gaaatgggcc aaagttgaag ccacagtggt tgaacaccgc
     1561 atcgagcatc acgcggatgc cccgggcatg gctttcgtcc accaggcgac gggcagtagc
     1621 ctcgtcgccg aaatgcgggt ctagcttgta gtagtctgta gtgtcgtact tgtggttgga
     1681 cggcgccagg aacacaggag tcaggtagat acacgacacg cccaggctgg ccaggtaatc
     1741 gagtcggccg atgatgccgg ccaggtcgcc gccggagaat gagcaagatg tggggcgagc
     1801 gccccatggg tcgactcgcg ccgggtcgct cgcctcatcg ccattggcaa atctatcaac
     1861 gaagatctgg tacacaacgg catcggcagc ccaggcaggc gggtcgtcgg cgccgtagtg
     1921 acgccagatg tatggataat gaaaacacca tgcccatggg ttgcatacct caggcgtcac
     1981 aggctcggcc gacaggccgc cctctccgta gtacattgtg gactggccat cgctcaggac
     2041 gaagtgatac atgaaccgcc cgtaccggag cctcatccgc cccgaccagt agtcgaacat
     2101 ggagtcgctt ccgactcggc gcaggccggt ctctttgggc tgcaggccga tgccgtacct
     2161 gtcgacgtag acgacgctaa ccgatttcag gtcggctctg gccgcccgaa agacgacttc
     2221 gatctcgtct tctcctaccg gataggccat ggggtcgtat ggcagatgca gcagcgcgtg
     2281 tttgagcaat gtttgttcga ctcctttgcc gctccttgac tgctccgccg gcaaactctg
     2341 ggctgagtca gtctattaca cagttctccc tggttcgagc aactcctcca tatcgcgggc
     2401 cagagagccg ggacgtccgg catgtgcttt tggtcgatag gcaaacccat gccggggtgc
     2461 atatggtgca ctagactcat tccagggaga gtgatcggct atgccagatg aggagaagga
     2521 agaggtgcag acacgtcaag ttccgtcgtg ccccggcggg ttcttgtatg agatcaaggc
     2581 aggcgacacg ttttacgctc tggctcggcg ttacgggacc acagtcgagg cgatcatggc
     2641 agctaatccc ggcgtggatc cgagaaggct tcagatcggc cagctggtgt gcataccggt
     2701 ggccgcgcct ccggtgccgc cagtgcctcc tcctgcggtg tgcccagggg gtacaccata
     2761 cgtcatacag gcgggcgata cgttctacag aatcgcgctc cggtacggaa cgaccgtcga
     2821 cgcgttgatc agggccaacc cgggcgttga tcctcgcaac ctccagatag gccaggtaat
     2881 ctgtgtgccg gtgccgattc cgattccgcc gccggctgta tgccctggcg gtgtgttcta
     2941 cacgattcgc gccggcgaca ccctttacac cattgcgctg cgctttggca ctacagtgaa
     3001 cgccatcatc gcagccaatc cgggtatcga tccatatcgc ctacgtgtag ggcagacgat
     3061 ctgcgttccg ctggccccag ttgtgcccag aacttgcgtg atggcgctgt cgccgtcggt
     3121 caccggcccg gcgcccacgg cgggcggggc agtgtggatg cggacggatg tgactgggca
     3181 gactcagata ctggtcgaag cgaccaatgt gcccccgccg gcgcaactcg gcgccaattg
     3241 gtacacggcc ctgttctcct gggaacgaac ctcgttcgaa gtgccgctag tggaagcgcc
     3301 aggcacaggc gtgtgggcgg ccactacagt ccaatcgctt cccgctcagt tctttgctgt
     3361 cggcagtgtg aacgtgttcc ccggtcccgt tcttggaggg ctagtagcca actgcaggta
     3421 gccggcaagt ggttgtcatg cagcagacgc gcagttcacc ggattcctca ggtagcccac
     3481 agagagcccg atgaagggag ccgcttcgcc ggctcccttt gccctgcctc atcctatgag
     3541 tatgcgctct tccgcaaggt gcactgtgga cggcttccgc ttgcgctggg caagggcgat
     3601 tacgaccgtc agcggcccga cgcggcccat gaacatcgtg gccattatga gggctcggcc
     3661 gatgtccgat aggttcgggg tgatgcccat ggtgagtccc acagtgccga acgcggacgc
     3721 ggtctcaaac agcacttcca aaaaacgcgc gttttcggta acggacaaga tcagcgtgac
     3781 cccggtcacc aaccccatgg ccagagtggc aatggtcatg gctttcagga tctcatcgtg
     3841 aggcaggcgc ctttcgtgca tctccacatc gtcccgtcct ctggcagtgc tgatcactga
     3901 ggcgatgagc accccaaagg tcgtcgtctt tatgccgccc cccgtggaac tgggagatgc
     3961 gccgatgaac atcaggataa tcatgacgaa aagcgttccg tctctgagat tgccgatatc
     4021 tagagtgttg aaacccgccg tgcgcggggt gatcgactgg aagatcgccc cgagcagctt
     4081 gcccgcccat gtcagggact tcagcgaatg tggattggaa agctccagcc cgagcacagc
     4141 tagggttccg ccgagcagca ggatccctgt gacttgaagc accaatctcg tgtggagaga
     4201 aagccggttt cctcggtgat gcccaatcag ctcgacgatc accgggaatc ccagcccgcc
     4261 ggtgattatc agcgagccta tggtgagcac gatcacagga tcggattgcc agccggtaag
     4321 gctggtgaaa ggcccggtca ccgtgccgaa caggtcgaac ccggcgttgc agaaggctga
     4381 gactgaatgg aatatcccgt aatacatgga cctggcaagc gggaattggg ccg
//
LOCUS       DAOTAC010000221         4388 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_16318_length_4388_cov_5.189015, whole genome shotgun sequence.
ACCESSION   DAOTAC010000221 DAOTAC010000000
VERSION     DAOTAC010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4388
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16318_length_4388_cov_5.189015"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="PLM74_09155"
     CDS             complement(<1..587)
                     /locus_tag="PLM74_09155"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HON43276.1"
                     /translation="MRARVIVGLVGAPIGVWVVWAGGIPFAVITLLVAGVAAYEYALM
                     CRTKTWSPSASLVVVASTMFVADAVFWSGRFTGLGLTFVLLGSLFLEVAKGRPVNSVG
                     GPALEVFGSLYCGWALGRFCLIRSSLGRAGLGLALLFVVLVWMNDMGAFFTGRAFGRR
                     KLAPGLSPSKTIEGAIGGVAWALAASVAFKALGDA"
     gene            complement(584..1348)
                     /locus_tag="PLM74_09160"
     CDS             complement(584..1348)
                     /locus_tag="PLM74_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HON43277.1"
                     /translation="MFDQRSAWREAVSNVPMHIAITMDGNGRWAQKRGKPRLYGHRVG
                     VETARTIVLAAPDMGVSHLTLFAFSTENWKRPPTEVQGIFRLLEEFFMRELDILASHG
                     ILVNVIGDRSGLPGFIQSVVARSEEMTRSGRRMVVNIALNYGGRWDIVHAMQALADDV
                     RAGRIEPGDITEEMVKSRLSTAGQPDPDLLIRTGGEQRISNFLLWQSAYSELYVTPVL
                     WPDFTPRHLNRAILEFQSRHRRFGALGPAGDGGDSN"
     gene            complement(1401..1943)
                     /gene="frr"
                     /locus_tag="PLM74_09165"
     CDS             complement(1401..1943)
                     /gene="frr"
                     /locus_tag="PLM74_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HON43278.1"
                     /translation="MSMAETRMKDVIESTVRDFAGVRTGRANPALLDRIVVDYYDTPT
                     PLNQIANVSAPEPRLLVVTAWDKSVLGKIEKAILKSDIGLVPTNDGNVIRMVVPHLTE
                     ERRRELVKVVRRIAEEKRVAVRGVRRDAIEQFRSMEKEGSITEDDLKRAQDEIQKVTD
                     KYISEIDKMLAEKEVEIMQV"
     gene            complement(1961..2680)
                     /gene="pyrH"
                     /locus_tag="PLM74_09170"
     CDS             complement(1961..2680)
                     /gene="pyrH"
                     /locus_tag="PLM74_09170"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HON43279.1"
                     /translation="MEMPRYKRVVLKLSGEAFAGGQGFGVEFDSVKWIAQQVADAHQL
                     GTEMAIVVGGGNIWRGAEASRRGMDRSTADYVGMLATVINSVVLQDALEDIGIPTRVL
                     TAIEMREIAEPYIRRRAVRHLEKGRVVIFGAGTGNPFFSTDTAAALRAAEVEADAILM
                     AKRVDGVYDCDPLTNPNATFFDKLSFIDVINRGLAVMDSTAASLCMDNNIPIVVFNLL
                     RDGNIRKALAGEPVGTYIGRD"
     gene            complement(2831..3430)
                     /gene="tsf"
                     /locus_tag="PLM74_09175"
     CDS             complement(2831..3430)
                     /gene="tsf"
                     /locus_tag="PLM74_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HON43280.1"
                     /translation="MEITASMVKELRERTGAGMMDCKKALLETGGDFEKAVIVLREKG
                     LAAAAKRSGRETSEGLIDAYIHMGGRIGVLIEVNCETDFVAKNAQFQALVHDLAMQVA
                     ASRPLYVSREQIPEDVAQREREIYRTQAINEGKPEKFADKIVEGRMAKFYQEVCLLDQ
                     PFIRDPEKSVADLIAENIGIIGENISVRRFVRFERGESL"
     gene            complement(3451..4254)
                     /gene="rpsB"
                     /locus_tag="PLM74_09180"
     CDS             complement(3451..4254)
                     /gene="rpsB"
                     /locus_tag="PLM74_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HON43281.1"
                     /translation="MAVVTMKQLLEAGVHFGHQTRRWNPKMKTYIFTSRNGIYIIDLQ
                     KTVRKVDEAYNFVRSVVQDGGSILFVGTKKQAHDTVAEEAQRCGMFWVNERWLGGMLT
                     NFATIRRRVDRMKMLERMEEDGSFEALPKKEVQKLVAEREKLQKRLSGIRDMRDIPSA
                     MFVIDPRKERIAVAEARRLNIPIVAIVDTNCDPDEVDYVIPGNDDAIRAVRLLTSKMA
                     DAVIEGREGLDQASPAEADAEADGGQYDGVNDESGEPEAYGDSQEAAIE"
BASE COUNT          867 a         1420 c         1130 g          971 t
ORIGIN      
        1 ctggcatctc ctaaggcctt gaacgcgact gaagccgcca atgcccaggc gacgcccccg
       61 atcgcccctt caatggtctt gctgggagac agccctggcg cgagttttcg gcgtccgaat
      121 gccctgccgg tgaaaaacgc gcccatgtcg ttcatccaca ccagaaccac gaaaagaagg
      181 gcaaggccca gcccggcacg gcccagagaa gaccggatga ggcagaaccg gccgagagcc
      241 cacccgcagt agagagaacc gaaaacctct agggctggtc ctcctacaga gttgaccggt
      301 ctgcctttcg caacctccag aaagagtgag cccagaagga cgaaggtgag cccgagaccg
      361 gtgaaccttc cgctccagaa taccgcatcg gctacgaaca ttgtcgaagc gaccacaacc
      421 aacgaagcgc tgggcgacca cgtcttagtt cggcacatca gggcgtattc ataggctgcg
      481 acacccgcaa cgagcagcgt tatcactgca aaagggatac cacccgccca caccacccaa
      541 acgccgatgg gcgcgcctac aaggcctact atcaccctgg ctctcaattg ctgtcgcctc
      601 catcgcccgc tggacccaat gcgccgaagc gtctgtgtcg cgactgaaac tccagtatgg
      661 cccggttcag gtgccgagga gtgaaatcag gccacagcac gggcgtgaca tacagctcgg
      721 agtaagcaga ctgccacaga aggaagttgc ttatccgctg ctcaccgcct gtgcgtatca
      781 ggagatccgg atcgggctgg ccggctgtgg aaagcctcga tttgaccatc tcctcggtga
      841 tgtcgccggg ctcgatgcgc cctgcgcgga catcatcagc cagcgcctgc atggcgtgga
      901 cgatgtccca cctgcctccg tagttcagag cgatgttgac taccatcctc cggcctgacc
      961 tcgtcatctc ctccgaccgc gcgaccactg actgaatgaa tccggggagt ccgctcctgt
     1021 cgccgatgac attgaccagg atgccgtgac tcgctaagat atcgagttct cgcatgaaga
     1081 actcctcaag aagccggaat atcccctgaa cttccgtggg cggacgtttc cagttctcag
     1141 ttgagaaggc gaagagcgtc aggtgactca cgcccatgtc cggggctgcc aagactatgg
     1201 ttcgcgcagt ctccacgccg acgcgatgcc cgtacaggcg gggtttcccc ctcttctgcg
     1261 cccatctgcc gtttccgtcc atggtgatcg ctatgtgcat aggcacgttc gagactgcct
     1321 ccctccaggc gctgcgctgg tcaaacatgc cacaaacccc ctcaatcgag ggggtgtgac
     1381 gaaacgggca caacagaggc ctacacctgc attatctcga cctctttctc ggccagcatc
     1441 ttgtcgatct cggatatgta cttatcagtc actttctgta tctcatcctg cgcgcgcttg
     1501 aggtcatctt ccgttatgga accctctttc tccatcgaac ggaactgctc tatggcgtct
     1561 cgacgaactc cccgcactgc cacgcgcttc tcctcagcga tccgcctgac cactttgaca
     1621 agctcgcgac ggcgttcttc ggtcagatga ggaacgacca tacgaatgac gttgccatca
     1681 ttggtcggaa ccagcccgat atcggacttg agtattgcct tttcgatctt cccaaggacg
     1741 gatttgtccc acgccgtcac cactaggagg cgcggttcag gcgccgatac attcgctatc
     1801 tgattgagcg gtgtcggagt atcgtagtag tcaacgacga ttcggtcgag cagagccggg
     1861 ttggctcttc cggtcctcac tcccgcgaaa tcgcggactg tggattcgat cacgtctttc
     1921 atcctagtct cagccatgga catttcgtcc ttgtacaaac ctaatccctc ccgatgtagg
     1981 ttcccacagg ctcgcccgca agcgccttcc tgatgttgcc gtcgcgcaac agattgaaga
     2041 cgactattgg gatgttgtta tccatacata acgacgcagc agtggagtcc atcaccgcga
     2101 gcccgcggtt gataacgtct atgaacgata gcttatcgaa gaacgtcgca ttcgggttcg
     2161 tcaaaggatc gcagtcgtac actccatcga cgcgcttcgc catgagaatg gcgtctgcct
     2221 ctacctcggc cgccctgagt gcggcagcgg tatctgtcga gaagaacggg ttccccgtgc
     2281 cggcgccgaa tatgacgacg cgtcccttct ccagatgcct cacagcccgg cgacggatgt
     2341 agggttcggc gatctcacgc atctcgatgg cggtcaggac cctcgtagga atacctatgt
     2401 cttcaagcgc atcctggagt accactgagt ttatcacggt agcgagcatg ccgacatagt
     2461 cggcggtgct cctatccata cccctccggc tggcctcggc cccgcgccat atgttccctc
     2521 cgcccaccac tatggccatc tccgtgccca actggtgtgc atctgcgacc tgctgagcta
     2581 tccactttac tgagtcgaac tcgactccga acccttgacc gccggcgaac gcctcgcctg
     2641 aaagcttgag cactactcgt ttgtacctgg gcatttccac gcaggtcacc ccttatgccg
     2701 attcgatacc tgctgacttt ctccttcacg aggcggaaac tccgcgtcag ggcagcctaa
     2761 actgggcaac cgataccggc gcactagcaa aagagagaac accgtggtgt tctctcttct
     2821 gcccattcta tcagaggctt tctccgcgtt cgaaacggac aaaccgcctc acactgatgt
     2881 tctccccgat gatgccgatg ttctcggcta tcagatcggc cacgctcttc tccgggtccc
     2941 tgataaaggg ctgatccaga aggcatacct cctggtagaa cttggccatc ctgccttcta
     3001 cgatcttgtc ggcgaacttc tcgggcttgc cctcgttgat tgcctgggtg cggtatatct
     3061 cgcgctctcg ctgtgccaca tcctccggaa tctgctcgcg cgacacgtag agcggccgag
     3121 atgcagcaac ttgcattgcg aggtcgtgta ccagagcctg aaactgcgca ttcttcgcca
     3181 caaagtcggt ttcgcagttg acctcgatca gcacaccgat ccttccgccc atgtggatgt
     3241 aggcgtcgat caagccttcg ctggtctctc tgccagaccg ctttgcggcg gctgccaggc
     3301 ccttctctcg gagtacaatg actgccttct cgaagtcgcc gcccgtctcg aggagtgctt
     3361 tcttgcagtc catcatgccc gcgcccgtac gttccctgag ctctttgacc atggatgcgg
     3421 tgatttccat ttgcaagcgt ccctccgtac ttactcgatt gcggcttcct gggagtcgcc
     3481 gtacgcctcc ggttcgccgg attcatcgtt gactccatcg tactgcccgc cgtctgcctc
     3541 cgcatctgcc tccgcgggag atgcctgatc gagaccttct cgcccttcaa tgacagcatc
     3601 ggccatcttc gaggtcagca gcctcacagc acgaatggca tcatcgttgc ccggtatcac
     3661 gtagtcgacc tcgtcagggt cacagttggt gtcgacgatg gcaacgatgg gtatgttcag
     3721 cctacgcgcc tcggcgacgg cgatcctttc ctttcgggga tcgatgacaa acatcgcgct
     3781 gggaatatcc ctcatgtcac gtatgccgga gagccgcttc tgcagcttct ctcgctcggc
     3841 gaccagtttc tgaacttcct tcttcggaag cgcttcgaag gatccgtcct cttccatgcg
     3901 ctcaagcatt ttcattcggt ccacacgccg gcgtatggta gcgaagttag tcagcatgcc
     3961 gcccagccag cgttcgttga cccagaacat cccgcagcgc tgagcttctt cagcgacagt
     4021 gtcatgcgcc tgcttcttag tacccacaaa caggatagat ccgccatcct gcaccactga
     4081 gcgaacgaag ttgtaggcct catccacttt tctgacagtc ttctggaggt cgatgatgta
     4141 gatgccgttt ctcgaagtga agatgtacgt cttcatcttc ggattccagc gtctggtctg
     4201 gtgaccgaaa tgcacgcctg cctcaagcag ctgtttcatt gtaaccactg ccatggatat
     4261 cacctccctc ccgcagcggt tttccctccg acttcttcat ctccgaaccc caccggcgct
     4321 tatccgccgg caccgcggcc cgaatcggaa gccgtgtgta gtcgcacaac ggttagtata
     4381 gcacagcc
//
LOCUS       DAOTAC010000222         4349 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_16482_length_4349_cov_5.090824, whole genome shotgun sequence.
ACCESSION   DAOTAC010000222 DAOTAC010000000
VERSION     DAOTAC010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4349
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16482_length_4349_cov_5.090824"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..1211
                     /locus_tag="PLM74_09185"
     CDS             117..1211
                     /locus_tag="PLM74_09185"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HON43282.1"
                     /translation="MFRDISYYKSDLDACMARVRAFFNCEPDAPGALIITFPSVLTLR
                     GPALNQYDFETDLCAYLDDYLEYQAQLLEIRRGIEDDWIPSVVPYMGIGEFSAFVAGE
                     IEFGEDTSWVKPVIRDKRDLDSLRLDPNNKWFTKLNFATRYMVQHIAPYRIPLGRGYY
                     SPLDLAWALRGEAIYVDFYEDPDFVRKLLEFSREATIWFARAQAAEIFAPQFVHELSA
                     WHCGPDRIALGEDISSLCSPSHYREFGAPYTQQVFDAFGIGEIHCHSAGPHVVPEFLK
                     LKRVRQIQIVADPNAARPVDILEDLMGGHPELFGRSPDLPVMSVDATADEIMDHYDLC
                     RRARIIFSVVTDEASGSNDAVRMFRNRQSL"
     gene            1262..2590
                     /locus_tag="PLM74_09190"
     CDS             1262..2590
                     /locus_tag="PLM74_09190"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HON43283.1"
                     /translation="MADAGSVHPVPIVAGALSALFAGVGQLYAGQRRRGIAYLLTEAA
                     FIFVLFTLLWGPLRGLITLMPQGRFTDSRHILIVGILAGFVVVAYALFHVANIADAVR
                     TASRAQAASQAARRPGAISLSASAVRRHAGVLGGTPLTPYLYIAPSLISAFFIIIVPL
                     VFGVSLAFTNYNLYHCPPAGRFDWIGLTNFAKLLKPGSTWRSQLDMVMTWNVTYAVLG
                     TVLAFAAGMGLALALQTHGLRFRRIFRMILMLPWAVPATVSIMVFLGLFNTSFGPINV
                     LLRSSGMRAIPWFQDRLWARASVLMTHVWISTPFNMSIISAALQSIPEEVYEAARVDG
                     ASRWQVFSRMTFPLLMLVVSPIALLTLAGNFNNFGIIYLLTGGGPAVAGSKGAGATDI
                     LMTWVYNLGFQQLQWSTASALAVLVFIFVVIFSMINFRLSGVLSQLKQEE"
     gene            2675..3559
                     /locus_tag="PLM74_09195"
     CDS             2675..3559
                     /locus_tag="PLM74_09195"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HON43284.1"
                     /translation="MASARVSAMAAPRRRRGRRGRAVEMVFVYLVLALACAIVLYPLL
                     WVIACSFRVSSGLISSSLIPAKPTLANYRRILTDPNVNFLRWFFNTIKVSALSSLGAL
                     AVTAPAAYAFSRMRFAGRRHVLICLMISQMFPSFMAIVALYTLLGWVGLIDSHLGLIL
                     LYAGGATPFSIWLLKGYFDSVPRSMEESAMIDGATKFQAFTKVLLPLARPILYVVGLI
                     NFIGPYAEYILAQSVITSGSRWTVAMGMRSLTMSSQFATDWPVFSATSVLTAIPIVIV
                     FLSAEKYIVSGLTQGAVK"
     gene            3649..>4349
                     /locus_tag="PLM74_09200"
     CDS             3649..>4349
                     /locus_tag="PLM74_09200"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HON43285.1"
                     /translation="MNGRLARTVAAAVAAIMLLLAGGAISLAADPIVVWCEPQKQATI
                     QAVAAEWTKQSGIPVQVEAVSVLETANKIQLAGPVGKGPDVFCCLSGQLGMLVTTGIA
                     APIDSALLDLSQFMKVGLDGATQNGKLYGVPYDISSVGLIYNKKMWPQPPATMDELIA
                     KSRDLKAKGKFGLLWPLENFYFTYAIMAGYGGYVFAPTANGWNTSDIGLANEGSVKAI
                     KLVKTLRDSGLIPVGT"
BASE COUNT          842 a         1262 c         1277 g          968 t
ORIGIN      
        1 caatggtgcg caacgacgct atattgacac gcaacggttc tccctctaat atagtagttg
       61 atgtgttgac tactatccat acctgagcgt ctggttaagg caaacgaggt ggtcgagtgt
      121 ttcgcgacat ttcgtattac aagtccgacc tggacgcctg catggcccgg gttcgggcct
      181 tcttcaactg tgagcccgat gcgccgggcg ctctgatcat aacattccct tcggttctta
      241 cactccgtgg ccctgctctc aatcagtacg acttcgaaac cgacctctgt gcctacctcg
      301 acgactactt ggagtatcaa gctcagctcc ttgagataag gcgcggcatc gaggatgact
      361 ggattccttc agtggttcct tacatgggca taggcgagtt cagcgcattt gtggccgggg
      421 aaatcgagtt cggggaggac acgagctggg ttaagcctgt catcagggac aagcgcgatc
      481 tcgactcgct ccgcctcgac ccaaataaca agtggtttac gaaactgaac ttcgctacaa
      541 ggtatatggt acaacacatc gcgccgtacc gcatcccgct tggacgcggc tattactccc
      601 cgctcgatct cgcctgggct ctgcgcggag aggcaatcta cgtggacttc tacgaggacc
      661 ccgactttgt gcgcaagctg ctggagttct ctcgggaagc gacaatctgg ttcgcgcggg
      721 cacaggctgc tgagatattc gcaccccagt tcgtgcacga actatctgca tggcattgtg
      781 gccccgaccg aatcgcgctc ggtgaggaca tatccagcct ctgctcaccg tcgcactacc
      841 gcgaattcgg cgccccctac acgcagcagg tcttcgacgc tttcggaata ggcgaaatac
      901 actgtcattc cgccgggccg catgtcgtgc cggagtttct caagctgaag cgcgtacggc
      961 aaatccagat cgtcgccgac cccaatgctg ctcggcccgt tgacattctg gaagacttga
     1021 tgggcggaca tcccgagcta ttcgggcgat caccggattt gcccgtgatg tcggtagacg
     1081 ccaccgccga cgagatcatg gaccactacg atctgtgccg gcgcgctcgc atcatattct
     1141 cagtcgtaac cgacgaagca tcaggatcca atgacgcagt ccgcatgttt cgaaacaggc
     1201 aatcactcta gcatgtgaac gtggcgccaa gtgaaccaag tcacaaaggg agggagccgc
     1261 catggcggac gcaggttccg ttcaccccgt ccccatcgtg gccggagcac tgtcagcgct
     1321 tttcgccgga gtaggtcaac tgtacgccgg gcagagaaga cgaggcatcg cttatctcct
     1381 cacggaagcg gcgttcatct tcgtcctgtt caccctgctc tggggccctc tgcgcgggct
     1441 aattactctc atgcctcagg gccggttcac cgactcgcgc cacatcctca tagtcggcat
     1501 tctggccggc ttcgtcgttg tcgcgtacgc attgttccac gtggccaaca tcgcggacgc
     1561 cgtgcgcacc gcctctcggg cgcaggccgc gtcgcaggct gctcgacgcc ccggcgcgat
     1621 ttctctatcc gcgtcggccg tcaggaggca tgcaggagtc ctcggaggca caccgctgac
     1681 tccctacctg tacatcgcgc cgtcgctcat atccgctttt ttcatcatta tcgttcctct
     1741 cgttttcggg gtctctctag ccttcaccaa ctacaatctg tatcactgcc ccccagcggg
     1801 caggtttgac tggataggcc tgaccaactt cgccaagctg ctgaagccgg gttccacgtg
     1861 gagaagccag ctcgacatgg tgatgacctg gaacgtgaca tacgcggtgc tcggcaccgt
     1921 tttggcgttt gccgcaggaa tgggcctggc actcgcgttg cagactcatg ggctcaggtt
     1981 ccgaaggatc ttccggatga tactgatgct gccgtgggca gttccggcca cggtgtcgat
     2041 tatggtgttc ctcgggctct tcaacacgag tttcggtccc atcaatgtgc ttctgcggtc
     2101 atcgggcatg cgtgcaattc cgtggtttca ggaccggtta tgggcacgcg ccagcgtctt
     2161 gatgacgcat gtgtggattt cgacgccctt caatatgtcc ataatctcgg ctgcgctgca
     2221 gagcatcccc gaggaggttt atgaggcggc gcgggttgac ggagcgtcgc gctggcaggt
     2281 gttttccagg atgacgttcc cgctcctcat gttagtggtg tccccaatag ccctcctgac
     2341 gttggccggg aatttcaaca acttcggcat catctactta cttaccggcg gcgggcccgc
     2401 agttgcggga agcaagggcg ccggcgccac cgacatcctg atgacgtggg tctataacct
     2461 ggggttccag caactccagt ggtctacagc atctgcactt gcagttttgg tgttcatctt
     2521 cgtcgtgata ttcagcatga tcaacttccg gctctcagga gtcctgagcc agctgaagca
     2581 ggaagagtag aacgagaggc tgaggctgag cgggaggctg aggctgggcg taagggcgag
     2641 gacgagggtg agggtgacga tgaggatagg gacgatggcg agtgcgaggg tgagtgcaat
     2701 ggctgcgcca cgcagacgcc gcggcaggcg cggcagggca gtggagatgg tcttcgtcta
     2761 cctggtgctg gcgctcgcgt gcgccatcgt cctctatccc ctgctctggg taatagcctg
     2821 ttcgtttcga gtgtcatcgg gcctcatcag ctcgagcctg ataccggcca aacccacgct
     2881 ggccaactac cgccggattc tcaccgaccc caacgtcaac ttcctgcggt ggtttttcaa
     2941 cactatcaag gtgtcggcac taagctcatt aggcgcactt gcggtcactg ctccggccgc
     3001 ttacgccttt tcgcgcatgc ggtttgcggg aaggcgccac gtgctgatct gtcttatgat
     3061 ctcgcagatg ttcccgagtt tcatggctat cgtcgccctc tacaccttgc tgggctgggt
     3121 agggctgata gactcgcacc tggggttgat cctgctgtat gcaggaggag cgacaccgtt
     3181 ttcgatctgg ttgctcaaag ggtacttcga ttccgtaccc aggtccatgg aggaatcggc
     3241 gatgatcgac ggggccacta agttccaggc gtttaccaag gtgctgctgc cgctggcccg
     3301 tccgatcctc tacgtagtcg gcctgatcaa cttcataggg ccatacgcag agtatatcct
     3361 ggcccagtcg gtgataacgt cgggcagcag atggacggtg gcgatgggca tgagaagcct
     3421 aaccatgtcg tcgcagttcg ccaccgactg gcctgtgttc tcggcgacgt cggtgttgac
     3481 ggccataccg atagtgatcg tatttctgtc ggccgaaaag tacatcgtgt ccggcttgac
     3541 ccagggagct gtcaagtgag ttcttcaggt gagttcctca ggccctgaaa aatgagtggg
     3601 attccaggcg taagacaatt acaaaggagg aacggtattg tgaaagccat gaatggacgt
     3661 ctggcgcgaa ccgtggccgc ggcagttgcg gctattatgc tccttttggc cggcggagca
     3721 atctcgctcg ctgcagaccc tatcgtagtg tggtgcgaac cgcagaagca ggcgacgatt
     3781 caggcagtcg ccgctgagtg gaccaagcag tctggcatac cagtgcaagt ggaagccgtg
     3841 tcggttctgg agactgcgaa caagatccag cttgccggcc ctgtgggcaa gggcccggac
     3901 gtgttctgct gcctctctgg tcagctgggc atgttggtga cgacgggcat cgccgctccc
     3961 atcgattcgg cgcttcttga cctgtcgcag ttcatgaagg tcgggttgga tggggccaca
     4021 caaaacggca agctgtatgg agtgccctat gacatctcat cagtgggcct catatacaac
     4081 aagaagatgt ggccgcaacc cccagcgacc atggacgagc tgatcgcaaa gtcccgcgat
     4141 ctcaaggcca aaggcaagtt cggactgctc tggccgctag agaacttcta cttcacctac
     4201 gctatcatgg ccggctacgg cggttacgtg tttgccccca cggccaatgg ttggaacaca
     4261 agcgacatag ggcttgccaa cgaagggtca gtgaaagcca tcaagctggt gaagaccctg
     4321 cgcgactcag ggctgatccc agtgggcac
//
LOCUS       DAOTAC010000223         4325 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_16593_length_4325_cov_3.888525, whole genome shotgun sequence.
ACCESSION   DAOTAC010000223 DAOTAC010000000
VERSION     DAOTAC010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4325
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16593_length_4325_cov_3.888525"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1094)
                     /locus_tag="PLM74_09205"
     CDS             complement(<1..1094)
                     /locus_tag="PLM74_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009064609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HON43286.1"
                     /translation="MRPSSTYRAALYMRLSKDDEGASESASISTQRKMLRNYAQENGF
                     AIYDEYVDDGWSGTNFERPDFRRMIQDIESRKVNLIITKDLSRLGRDYIATGQYTEIY
                     FPSKGVRYIAINDGYDSDSPYTDIAPFKNVVNEMYARDISRKIRSAFATKMQDGSYIG
                     NFAPYGYRKDPTNKNHLIPDEKTAPIVQEIFQMAAKGSRPVEIAHHLNGKGVPSRSVY
                     RRSRHPHLGAERRSDRSEWTSGAVTKLLGNIVYLGHIAQGKTTKVSFKSRTTIRNPRR
                     DWIVVMNTHEPLIDQDTFDMARRRTQCRTCKKKGTFFNLFSGIARCADCGRNMSTVST
                     RKKGSPANLACGGYKLRGGRDLLMCRPDPV"
     gene            complement(1091..1249)
                     /locus_tag="PLM74_09210"
     CDS             complement(1091..1249)
                     /locus_tag="PLM74_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43287.1"
                     /translation="MRTMKPTGQRSRPPGLGGRKAIYTVEIVSPCEKTAKESAKAKGP
                     QRPSKEDT"
     gene            1442..2356
                     /locus_tag="PLM74_09215"
     CDS             1442..2356
                     /locus_tag="PLM74_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012198303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HON43288.1"
                     /translation="MEYISVKQAAEKWGLSDRRVRVLCEHGKIAGARKEGRSYLIPSD
                     ALKPADGRRLRGRLVPKEFASLLARVDSAKEELDRRRPLTVGEVKRLQDEFLIEFTYS
                     SNAIEGNTLTLKETAMVLEGITIDRKPLKDHLEAVGHRDAFRYVQQLVAAKAPFSERI
                     IREIHSLVLLDKPEDRGVYRRIPVRIMGAHHEPPQPYLVPVQMERLVAESAHDKRHPV
                     HGAALFHLEFEGIHPFVDGNGRTGRLILNLTLMQHGYPPINVKFADRRRYYDCLDTYF
                     RDHDEEPMVTMVAEYVEERLKQYLAALG"
     gene            complement(2275..3579)
                     /locus_tag="PLM74_09220"
     CDS             complement(2275..3579)
                     /locus_tag="PLM74_09220"
                     /inference="COORDINATES: protein motif:HMM:NF014474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HON43289.1"
                     /translation="MLSRPAKRADGRAAYLSTHHCPDEHVIALAGNPNTGKSTVFNAL
                     TGLRQHTGNWPGKTVDLAFGRVDLGGRRAVVVDLPGAYSLLACSEDESIARDFLCEQG
                     PDAVVVVVEAGCLERNLSLVLQIIELTPRVIVCVNLIDEANRLGIQVDSEALERELGV
                     PVTLTAASSGRGLDQLKRTVADLIDGRLQPKPLPVAYPPDIEDASRELQSKLPTLPSA
                     VSRRYVALRMLDTDPAIRERIVESIYCRAKSISDRAVSRIPSSAPASRVATESGIDDV
                     LTSRWLGFPVMLALLGGVFWITLVGANYPSALLAKVLFHMEAWLSAAFRAAHAPDWLY
                     GAVVLGAYRTMAWVVSVMLPPMAIFFPLFTLLEDLGYLARVSFNMDWCFKKCGAHGKQ
                     SLTMWIDSRPCAEATVHPSAARYCFSRSSTYSATIVTMGSSS"
     gene            complement(3573..3878)
                     /locus_tag="PLM74_09225"
     CDS             complement(3573..3878)
                     /locus_tag="PLM74_09225"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HON43290.1"
                     /translation="MESDSDREPKARPLTDLPLGQTGVIRSVNATGMVRNRLLDLGFL
                     PGTEVSAVRRAPMGDPTAFRIRGAMLALRAQDSSLVTVTPVDGLQMDAETETGAKTC"
     gene            complement(4026..>4325)
                     /locus_tag="PLM74_09230"
     CDS             complement(4026..>4325)
                     /locus_tag="PLM74_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43291.1"
                     /translation="DSGHTDAFDRSFGAGVAVRPMDNLLIAADVIWKDGIDARVGGEF
                     RLGKFAVRAGGGYRDDKLSFSAGAGFLLPSFSIDYAYQTHSILPDSHRLSLCVKF"
BASE COUNT          827 a         1227 c         1327 g          944 t
ORIGIN      
        1 tctactggat cgggccggca catcaggaga tcccgaccgc ctcgcagctt gtagcctccg
       61 caggcgagat tggcggggga acctttcttc cttgtgctca cagtggacat attccgcccg
      121 cagtcggcgc acctggctat tccggaaaag aggttgaaga aagtgccttt cttcttgcat
      181 gtccggcatt gggtgcgcct ccgcgccatg tcgaaggtgt cttgatcaat gagcggctca
      241 tgggtgttca tgactacgat ccagtctctc ctgggattgc gtatggtcgt gcggctcttg
      301 aatgacacct tagtcgtctt gccctgtgct atgtggccga gatagacgat attcccgagc
      361 aacttcgtca cggcgccgga ggtccattcg cttcggtcgg agcggcgttc ggcgcccaga
      421 tgcggatgac gggaacggcg atacacggag cgagacggaa cgcctttgcc gttcagatgg
      481 tgggcgatct caacaggcct gctgcctttg gcggccatct ggaagatctc ctgcacgatg
      541 ggggcggtct tctcgtcggg tatcagatgg ttcttgttag tggggtcttt cctgtagccg
      601 tagggggcga agttgcctat gtagctgccg tcctgcatct tcgtggcaaa agcgctgcgg
      661 atcttcctgg agatgtccct tgcgtacatc tcattgacaa cgttcttaaa cggggcgatg
      721 tcggtatagg ggctgtcgga atcatagccg tcgttgatgg cgatatagcg cacgcctttc
      781 gatgggaagt agatctcggt atactgccct gtggcgatgt aatccctgcc caagcgcgat
      841 agatccttcg tgataatgag gttcactttt ctgctctcga tgtcctgtat catcctccta
      901 aagtccgggc gttcgaagtt agtgccgctc cagccgtcgt cgacatactc atcgtagata
      961 gcgaacccgt tttcctgcgc ataattcctg agcattttcc gctgtgtgct gatgctcgcg
     1021 ctttcagagg cgccttcgtc atccttcgac aacctcatgt aaagcgcagc tcgataggtt
     1081 gatgatggtc tcatgtgtcc tcctttgagg ggcgctgcgg gcccttggct tttgccgatt
     1141 ccttggcggt tttttcgcac ggtgacacga tttccacggt gtagatggcc tttcgtccgc
     1201 ctaatcccgg aggacggctc ctctgacctg tgggcttcat cgtcctcata tcggcacctc
     1261 ccgaagaaac ctatgaaggg aggcgcgtcg ttttgcctgt ccaggccggg atctagcttg
     1321 gggaatatta tcgcatgaga tcgcttagta tgcgttgata ttattccgat gctatggcat
     1381 actgctgttg cgaagaagaa tcactcccaa gtaacacgcg aggcattgtg aggtgacgcg
     1441 tttggagtac atcagcgtga aacaggcggc agagaagtgg gggctgtccg atcgccgtgt
     1501 tcgcgtgctg tgcgaacatg ggaagatcgc gggcgccagg aaagaggggc gttcgtacct
     1561 gataccgtca gacgcgctca agccggcaga tggccgaagg ctgcggggaa gactggtacc
     1621 gaaggagttc gcctcgttgc ttgcacgcgt cgactccgca aaggaggagc tggacaggcg
     1681 ccgccctctg accgtcggcg aggtgaaacg gctgcaagac gagttcctga tcgagttcac
     1741 atacagctcc aacgctattg aaggaaacac cctcacactg aaagagacgg cgatggtgtt
     1801 ggaaggaatc acgatcgacc gcaagccgtt gaaggaccac ctagaggctg tggggcatcg
     1861 cgacgccttc cggtacgtgc agcaactcgt cgcagccaag gcgccgtttt cggagagaat
     1921 catcagagag atccattccc tcgtgcttct tgataagccc gaggataggg gggtgtatcg
     1981 acgcatcccc gtgcgcatta tgggcgctca ccacgagccg ccgcagccat atctcgtgcc
     2041 ggtgcagatg gagaggctcg tcgcggagtc tgcacacgac aagcggcacc ccgttcatgg
     2101 cgcggctctg ttccatcttg aattcgaggg cattcacccc tttgtagacg gcaatggacg
     2161 gaccggcagg ctgattctga atctcactct gatgcagcac ggttatccgc ccatcaatgt
     2221 gaaattcgcc gaccggagac gttactatga ttgcctggat acctatttca gggatcatga
     2281 tgaggaaccc atggtcacaa tggttgcgga gtacgtcgag gaacggctga aacagtatct
     2341 tgccgcactt ggatgaactg tcgcttctgc acacggtctg ctgtcgatcc acatggtaag
     2401 cgactgcttg ccgtgggcgc cgcacttctt gaagcaccag tccatgttga agctgaccct
     2461 ggccaagtag cccaagtctt caagcagcgt gaatagggga aagaagatcg ccatgggggg
     2521 cagcatcacc gatactaccc aggccatggt gcggtatgcg cccagaacga ctgcaccgta
     2581 tagccagtcg ggggcgtgtg ccgcccggaa tgcagccgac agccacgcct ccatatggaa
     2641 gagtaccttg gccagaaggg ccgacgggta gttcgccccc accaatgtta tccagaacac
     2701 ccctccgagc agcgccaaca tcacggggaa ccccagccag cgcgatgtga ggacatcgtc
     2761 gattccggat tccgtggcga ctctcgatgc cggcgccgat gatggtattc ggcttacggc
     2821 tctgtctgat atggacttgg cgcggcagta gatcgattcc acgatccttt cgcgaatcgc
     2881 gggatcagta tccagcatac gcagcgccac gtaccttcgg ctgacggcgg agggcagtgt
     2941 gggcagcttg gactggagtt cgcgcgaagc gtcttcgatg tccgggggat aggcgacagg
     3001 cagcggtttc ggctgcaggc gtccgtcgat tagatctgcg acagtgcgct ttagctgatc
     3061 gaggccgcgg ccactcgatg cggcagtgag tgtcaccggc actcccaatt cgcgctcgag
     3121 cgcttcgcta tcgacttgta tgccgaggcg attggcctca tctatcaggt tcacgcacac
     3181 gattaccctg ggagttagct cgattatctg aaggaccaga ctcagattgc gctcgaggca
     3241 tccggcttcg actaccacga cgacggcgtc ggggccttgc tcgcagagga agtcgcgagc
     3301 tatggattca tcttcggaac aggcgagaag cgaatacgcg ccgggcagat ccacgactac
     3361 cgcgcgcctg ccgccgaggt cgaccctgcc gaacgctaaa tccacggtct ttcccggcca
     3421 gttgccggtg tgttgccgaa ggcccgtcaa agcgttgaac acggtgctct tgccggtatt
     3481 tgggtttcca gccagcgcaa tcacgtgttc atcggggcaa tggtgtgtcg aaaggtacgc
     3541 ggcgcgtccg tcggcgcgtt tggcgggtcg gctcagcatg tcttggcccc cgtttccgtt
     3601 tctgcgtcca tctgcaggcc gtcgacgggc gtgacagtca ccaaagagga gtcctgtgct
     3661 cggagtgcga gcatggcgcc tctgatcctg aatgctgtgg gatcgcccat cggggcgcgc
     3721 cgcactgccg acacctctgt cccggggaga aagcccagat ccaacaggcg gtttcgcacc
     3781 atgccggttg cgttcacgga gcgtataacg cccgtctggc cgaggggcag gtccgtcagg
     3841 ggtctggcct tgggttcgcg gtcggaatcg ctttccatgc aggcctcctg atacgcgtcg
     3901 gatcgcgcca ttctggtctc atgaagcggc gccctgacag catatgtgcc cgaggtggct
     3961 attgtgccgc gggcagaaga agggacggct gatgccgtcc cctcgatctg ccgcctgacc
     4021 gatcgctaga acttgacgca cagcgacagc ctgtggctgt caggcaggat gctgtgagtc
     4081 tggtaggcat agtcgatgct gaacgatggc agtaggaagc cggcgccggc cgagaacgat
     4141 agcttatcgt cgcgatagcc gccgcctgcg cgcacggcga acttgcccag ccggaattcg
     4201 cctccgactc gcgcgtcaat gccgtccttc cagatcacat ctgcagctat tagcaggttg
     4261 tccatggggc gcactgcgac tccggctcca aaggagcggt cgaaggcatc agtgtgcccg
     4321 ctgtc
//
LOCUS       DAOTAC010000224         4310 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_16645_length_4310_cov_4.132080, whole genome shotgun sequence.
ACCESSION   DAOTAC010000224 DAOTAC010000000
VERSION     DAOTAC010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16645_length_4310_cov_4.132080"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..953)
                     /locus_tag="PLM74_09235"
     CDS             complement(<1..953)
                     /locus_tag="PLM74_09235"
                     /inference="COORDINATES: protein motif:HMM:TIGR01945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit C"
                     /protein_id="HON43292.1"
                     /translation="MPRGSFRGGIHPPENKSSTSRLPIVAAQQPSKVVVLLNMGTSCA
                     LSSVVAKGDSVRKGQKIGDPAGRFGVPSHSPVSGKVTAVGVVRTGLGTLDQAITIEPD
                     GQDTLDESCVPVENVDRLSQAEIVDIIREAGIVGMGGAQFPTHIKLTLPPNVKVDTLL
                     INGAECEPYLTADHRLMVERPGDVVRGTQILMKAIGVDRAVIGIEDNKPDAVDALSRA
                     VAEASAGGAVEVAAVRTHYPQGSEKHLIKSVLGREVAPGCLPFSVGVVVNNVGTAVAV
                     ADRFDRGLPLIERVVTVTGSGVAQPANIMMKIGTPAIELIE"
     gene            complement(1262..2986)
                     /locus_tag="PLM74_09240"
     CDS             complement(1262..2986)
                     /locus_tag="PLM74_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HON43293.1"
                     /translation="MSDITLTIDGRTVHTAPGTTILEAARSVGIKIPTLCWHEDLGAP
                     SACRVCVVEIVGSRIMQPACSYPVSQGIMVRTSSPAVREARRMAVELLLAHHPDDCLS
                     CARNLRCELQQLASDLGIREIRFERVERGLAKDESSPSIVRDPEKCINCRRCVEACEK
                     IQGVAALSTVNRGFDSVVMPAYESGLMDVVCVMCGQCTLACPTGAITERDDTARVWAA
                     LADPSKHVVVQTAPAIRASLGEELDLPAGSIVTGKMVAALRRLGFDRVFDTDFTADLT
                     IMEEGHELLQRLTSGGTLPLLTSCSPGWIKFIEHFYPELLPHVSTCKSPQQMFGALAK
                     TYYAEKAGICPADMFVVSIMPCTAKKFEAQRPEMTASGNPDVDVALTTRELGRMLREA
                     GIDFDSLPEEDFDAPLGISTGAGAIFGATGGVMEAALRTVYEVVTGRELPAESLEFHD
                     VRGLEGVKEISVTLNGNSVNVAVAHGLANARKVLDRMRAGQAPWQFVEIMCCPGGCVG
                     GGGQPIGTVMNTRAERARALYDVDRGMELRSSHRNPGVAALYEEFLGRPLSKRSHELL
                     HTHYTPRR"
     gene            complement(3004..>4310)
                     /gene="nuoF"
                     /locus_tag="PLM74_09245"
     CDS             complement(3004..>4310)
                     /gene="nuoF"
                     /locus_tag="PLM74_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012896186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HON43294.1"
                     /translation="QIQKRIVLDNVGIIDPESIEEYIARDGYLAAGTALTGLSPEEVI
                     ETIKRSGLRGRGGAAFPTGLKLGFTARAEADQKYIICNADEGEPGTFKDRLILEGDPH
                     KVIEGMVIMGYAVGATRGYIYIRGEYYVSIDRVERAINQARWLGLLGRNLFGSGFDFD
                     IEVKKGAGAYVCGEETALIESMEGKRGEPRQKPPYPGTSGLWGKPTVVNNVETIANIP
                     PIILHGAEWYRSFGTSTSPGTKVFTLTGNVNNKGLIEVPMGITLREVIYGVGGGIPGG
                     RSFKLAQTGGTAGGCVADAHLDVPMDYESMQSAGSALGSGALLIIDDSHCIVDVACSL
                     LHFFAHESCGQCTPCREGTARLVQIFNKLREFRASQADLDLAMELARVMQGSSLCALG
                     QSVIVPLGTIMEHFHGEIQAHLHGSCPAGKCSLSGATSASAS"
BASE COUNT          843 a         1413 c         1162 g          892 t
ORIGIN      
        1 tgttctatca gttcgatggc gggcgtgcct atcttcatca tgatgttggc cggttgcgcg
       61 actcccgacc ctgtcacagt tactactctc tctatcaacg gaagcccgcg gtcgaacctg
      121 tcggccacgg caacggccgt tccgacattg ttgaccacga cccccacgct gaatggcagg
      181 catccgggag cgacttccct gcccagcacc gatttgatga ggtgcttttc agaaccctgc
      241 gggtaatgag tcctaacagc ggccacctcg acggccccgc ctgccgacgc ctcggcaact
      301 gccctgctaa gcgcatcgac ggcgtcaggt ttgttatcct caattcctat cacggctcga
      361 tccacaccaa ttgccttcat gaggatctga gtgccacgta ctacgtcgcc cgggcgctcc
      421 accatcagtc tgtggtcggc cgtgaggtac ggctcgcact cagccccatt gatcagcaga
      481 gtgtcgactt tcacatttgg gggcagagtc agcttaatgt gggtaggaaa ctgcgcgcct
      541 cccattccca ctattcccgc ttcgcgaatt atgtcgacta tctccgcctg cgaaagcctg
      601 tcgacattct ccacaggcac gcatgattcg tccagcgtgt cctggccgtc aggttcgatc
      661 gtgatcgcct gatccaacgt gcccaggccg gtgcgcacga cgccgacagc cgtcaccttg
      721 cccgataccg gcgaatgcga tggcacaccg aacctcccgg caggatctcc gatcttctgg
      781 cccttccgca cagagtcgcc ctttgcgacc accgacgaca gagcacagct agtgcccatg
      841 ttgagcagca cgactacctt cgacggctgc tgtgccgcca ctatcggcaa cctgctagtg
      901 cttgacttgt tctccggagg atggatacct cctcggaacg accctctggg catgcacctc
      961 actcctttgt acacaacccc taagctgtaa ccccggacgc actctccaag ccacttggca
     1021 gtacagcatc gaaccctcca gccagcctgc ctaccgcccg actgagagtt cgacgcgagc
     1081 caatcaaagc cggtaatatt gtataacacg cagtacactt gtgcaacaat tctacagctt
     1141 aacagctaat tgaacctgcc tggcaattcg accctcaaaa agggcagaaa gcctgctctc
     1201 ccggcgtgcg agagagatgc cctctgccct gctttctccg tcaagcgtcg gcctcgccgc
     1261 cttaccgcct tggcgtgtag tgagtgtgca gtaattcgtg tgatcgcttg gataggggtc
     1321 gacccaggaa ctcctcgtaa agcgccgcta cccctggatt tctgtgcgat gagcgcagtt
     1381 ccatgcctcg atccacgtca tacagcgctc tggcacgctc ggctcgggtg ttcatcacag
     1441 tgccgatggg ttgcccgccg ccgcccacac acccgcccgg gcagcacatt atctccacga
     1501 actgccaggg cgcctgcccg gcccgcatac ggtctagcac cttccgggca ttagctagtc
     1561 cgtgagcgac tgcaacattc acactgttgc cgttcagggt taccgagatc tctttgactc
     1621 cctcaagccc cctgacgtca tggaattcga ggctctcggc gggcaactcc ctgcctgtaa
     1681 cgacctcgta caccgtgcgc aaagccgcct ccataacacc acccgtggcg ccgaatatcg
     1741 ccccggcgcc ggtggatatg ccgagaggcg cgtcgaaatc ctcttcgggc aagctgtcga
     1801 agtcgatgcc cgcctcacgc agcatccgcc ccagctcgcg agtggtgagc gccacatcga
     1861 cgtcagggtt tccgctggca gtcatctccg ggcgctgggc ctcgaacttc ttcgccgtgc
     1921 agggcatgat ggacactacg aacatatccg cagggcatat gccagctttc tcggcgtagt
     1981 atgtcttggc cagggcccca aacatctgtt gaggagactt gcacgtggac acatgcggca
     2041 gaagctcggg atagaaatgc tcgatgaact tgatccatcc ggggctgcat gaagtgagca
     2101 ggggcagtgt accgccgctc gtcagccgct gcaggagctc gtgcccttcc tccatgatgg
     2161 tgagatcagc tgtaaagtcg gtatcaaaca cgcggtcaaa accgagcctc cttagcgcgg
     2221 caaccatctt ccctgtcact attgagccgg ccggcagatc tagttcctcg cccaaggaag
     2281 cacggatggc cggagcggtc tggaccacta catgcttgga agggtcagcg agtgccgccc
     2341 atacgcgcgc cgtgtcgtct ctctccgtta tggcgccggt cgggcatgcc agagtgcact
     2401 ggccgcacat tacgcacacc acgtccatga ggccagattc gtaggcgggc atcaccactg
     2461 agtcaaaccc acggttcacc gtggaaagcg ccgcaactcc ctgaatcttc tcacaagcct
     2521 ccacgcaccg tcggcagttg atgcacttct cagggtcacg cactatggac ggagacgact
     2581 catctttggc tagacctcgc tcgacccgct cgaaccttat ctccctgatt ccaaggtcag
     2641 acgcgagctg ctgcagttcg caccggaggt tcctggcaca agacaggcag tcgtcgggat
     2701 ggtgggccaa aagcagttcc accgccatcc tacgagcctc cctcacggct ggggagctgg
     2761 tacgcaccat tatgccctgt gacaccggat acgagcaggc aggctgcatt atgcgcgaac
     2821 ctacgatctc gactacacat acgcggcagg ccgacggagc gccaaggtcc tcatgccagc
     2881 atagtgtagg gatctttatg ccgacactgc gggccgcctc gagaatggtc gttcccggcg
     2941 cggtatgcac cgtccgccca tcgatcgtca atgtaatatc actcatcctc tgtcggcctc
     3001 ctttcagctg gcactggcgc tcgtggcgcc ggatagcgag catttgcccg cggggcagga
     3061 tccgtgaaga tgcgcctgga tctcgccgtg gaaatgctcc ataatcgtgc ccagcggcac
     3121 tattaccgat tggcccagag cgcacagcga cgaaccttgc atcactcgag ccaattccat
     3181 ggcgaggtcc agatccgcct gagaggcgcg gaactcgcgc agcttgttga agatctgtac
     3241 aagcctggct gtgccctccc tgcagggagt acactgccca cacgattcat gcgcgaagaa
     3301 atgcagcagg gaacaggcca catccactat gcagtgactg tcgtcgatta tgaggagcgc
     3361 cccggatccc agggcggacc cagccgactg catgctctcg taatccatag gtacatccag
     3421 atgggcatcg gctacgcatc cgcccgctgt gccgccggtc tgggccaact tgaacgaccg
     3481 accgcccgga atgccgccgc cgactccgta gatcacctca cgcagcgtga tgcccattgg
     3541 cacttcaatg agtcccttgt tattgacatt gccagtgaga gtgaacacct tggtgcccgg
     3601 cgatgtagac gtgccaaagc tccgatacca ttcggcgccg tgtaggatga tcggcgggat
     3661 gttggcgata gtctccacat tattgaccac ggtaggcttg ccccacagcc ctgaagtgcc
     3721 tgggtacggc ggcttctgac gtggttcgcc gcgcttgccc tccatggact cgatgagcgc
     3781 ggtttcttcg ccacatacgt aggcgccggc gcccttcttg acttcgatgt cgaagtcgaa
     3841 tcccgaacca aacagattcc ttcctaagag gcctagccag cgcgcctggt ttattgcgcg
     3901 ctcaacccgg tcgatcgaaa cgtagtactc gccgcgtatg tagatgtacc cacgagtggc
     3961 gccgacggca tagcccatga tgaccatgcc ttcaatcacc ttgtgagggt cgccttccag
     4021 tatcagacgg tctttgaacg tccccggctc gccttcgtcg gcgttgcaga ttatgtactt
     4081 ttggtcggct tcggcccttg ccgtaaagcc cagcttgagg cctgtgggaa atgctgctcc
     4141 cccgcgccct cgcaggcccg aacgcttgat ggtttcgatc acctcttccg ggctaagccc
     4201 ggtaagcgca gttcccgccg ccagataccc atcgcgagca atgtactcct cgatgctctc
     4261 ggggtcgatt atccctacat tgtcgagcac aatgcgcttt tggatctgga
//
LOCUS       DAOTAC010000225         4296 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_16713_length_4296_cov_4.143362, whole genome shotgun sequence.
ACCESSION   DAOTAC010000225 DAOTAC010000000
VERSION     DAOTAC010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4296
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16713_length_4296_cov_4.143362"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="PLM74_09250"
     CDS             <1..490
                     /locus_tag="PLM74_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HON43295.1"
                     /translation="GKAYFTYVYACQVAEVAVDTQTGQVVVERVTAAHDVGRAINPQT
                     VEGQIYGGVAMSTGYGLLEEVELNDGITRTRNFDEYLIPTAMDVPEIKPIIVENADPY
                     GPYGAKTIGEPTCELGAAAIANAIFHATGTRVRALPMDMERVLLGRTLRPMRGRRGSE
                     AR"
     gene            496..1401
                     /locus_tag="PLM74_09255"
     CDS             496..1401
                     /locus_tag="PLM74_09255"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HON43296.1"
                     /translation="MQVYRPLCLDDALRYLSECAEAQGNPARPIAGGTDLIVSARHAA
                     TAGAAEQPAGWRVVDISGLPEISGICVDGDEVCIGAATTHTEIEESAVIDKSAPFLAE
                     AAATIGSRQIRNRATLGGNVMNASPAADSLPPLVALSATATLASVRGRRVVPVAELVT
                     GPYRTVAVPDELLVDVRFRALEPKAQSAFIKVGRRRALAISRLAAAVAMLFDSDAKIV
                     DTRISAGAAFPTPRRIGQAEQVLLGREYKAELAEEAGREAARAMVCATGVRWSTEYKE
                     PVLAAIIARAIRQCAAKEGLDVWPE"
     gene            1482..2003
                     /locus_tag="PLM74_09260"
     CDS             1482..2003
                     /locus_tag="PLM74_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009115131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HON43297.1"
                     /translation="MKVNGHDVTVSVPGCERLLDTIRERLHLTGTKEGCGVGECGACT
                     VILNGQAVNSCLVLTAQCDGAEVMTVEGLAEDGEMHALQRAFIEHGAVQCGFCTPGML
                     MSAKALLDADPHPDRDEIRQAIAGNLCRCTGYEQIVQAIESVARQAGPSEDRGLRKDH
                     VEGGCESDGGQGS"
     gene            1984..3330
                     /gene="ssnA"
                     /locus_tag="PLM74_09265"
     CDS             1984..3330
                     /gene="ssnA"
                     /locus_tag="PLM74_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003358040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HON43298.1"
                     /translation="MVDKVPDLIIGNGVVVTCDSKSQVIEDGAVAVAGDRVADIGPTQ
                     DLVQRYPGAKFEDVGGRLVMPGMTCTHMHLYSTFARGMQLDGEPARTFGQILERLWWR
                     LDRALTEEDVWMSAMMPLIWCVRNGTTTILDHHSGPNAIPGSLDVIADAVNRVGMRAS
                     LAYEVSDRDGEKARDQGIAENVRFIEACKRNRSPLLRATFGLHASFTLSDETLKKCVD
                     APGAREAGFHIHTAEGIEDLIQSIRQYGQPVVKRLDKMGLWNDRSLAVHCVHISPEEI
                     EILRERDVNVVHNPESNMGNAVGAAPALEMIKRGVRVGLGTDGYTYDMFESIKVGNLI
                     HKHKEANPQAAWAEIPLMAFENNSKILAKYWDVPLGVLTPGAYADVIVVNYWAPTPIT
                     SSNWYSHVLFGVSGGMVDSTMVGGKFLMRRGELLTVNEQEMAAKSREMARGVWERA"
BASE COUNT          914 a         1135 c         1456 g          791 t
ORIGIN      
        1 ggggaaggcc tacttcacct atgtctacgc atgccaggtg gcggaggttg cagtcgatac
       61 gcagacggga caggtggtcg tggaacgtgt gactgccgcc cacgatgtag gccgcgctat
      121 caacccacag acggttgaag ggcagatata cggcggtgtc gcaatgagca ccggatacgg
      181 tctgcttgaa gaagtcgagc tgaacgatgg aataacgagg acgcgcaatt tcgacgagta
      241 cctgatacct acggccatgg acgtgccgga gatcaagccg atcatcgtcg agaacgcaga
      301 tccttacggc ccctacgggg ccaagactat aggcgaaccc acgtgcgagc tgggcgcggc
      361 ggctatcgcc aacgcgattt tccacgccac gggtacgagg gtgagggcgc ttcccatgga
      421 catggagaga gtgctcctag gcagaacgct caggcccatg cggggacgaa gggggagtga
      481 ggcccggtga tcgagatgca agtctacagg ccgttatgtc tggacgacgc cctgcggtac
      541 ttgtcggagt gcgccgaggc ccagggcaat cctgcacggc ccattgcggg cggtactgac
      601 ctcatagtgt cggcaaggca tgcggcgacg gcaggggcgg cggagcaacc ggccggctgg
      661 cgggtggtcg atatatccgg gctgcctgag atatccggga tatgcgttga tggtgatgaa
      721 gtgtgcatcg gcgctgcgac cacgcacact gagatcgagg agtcggcagt gatcgacaaa
      781 tcggcgccgt ttctggcgga ggcagcggcg actatcgggt ctcgccagat ccggaacaga
      841 gctactctcg gcgggaacgt aatgaacgcc tctcctgccg ccgacagcct tccgccgctc
      901 gtggcgcttt ccgcaacggc gactttagcc tcggtgcgcg gcaggcgtgt ggttcccgtg
      961 gctgaactgg tgaccgggcc atacaggacg gtcgcagtcc ccgacgagct gctggttgac
     1021 gtgagatttc gggcgctgga accgaaagct cagtcagcct tcataaaggt gggccggagg
     1081 cgcgcgcttg ccatatctag gctggcggct gcagtcgcca tgctgttcga ctcggatgcg
     1141 aagatcgtcg atacgcgtat ctcagccggg gctgctttcc cgacgccgcg ccgaatcggc
     1201 caggctgagc aggtcctgct gggcagggaa tacaaggctg agcttgcaga ggaggcgggg
     1261 cgcgaggcgg ctcgggccat ggtgtgcgcg actggagtcc gctggtccac agagtacaag
     1321 gaacccgtcc ttgcagccat tatcgccagg gcgatcaggc agtgcgcagc gaaggagggg
     1381 ttggacgtat ggccggagta accggaaccg gaaccaaaac cgatgccaat gcgcgaggcg
     1441 gttgctgtgg gcatgaagcg gccgccacag tagatgtgcg catgaaggtc aacgggcacg
     1501 acgtgacggt gagcgtgcct gggtgcgagc gcttgctcga cacgatcagg gagaggctgc
     1561 acttgaccgg gaccaaggaa ggctgcggag ttggcgagtg cggggcgtgt accgtgatac
     1621 tgaacgggca ggcggtcaac tcctgcctgg tactgacggc gcagtgcgat ggggctgagg
     1681 ttatgacagt ggaagggctg gccgaggatg gggagatgca cgccctgcag cgggccttca
     1741 tagagcacgg cgcggttcag tgcgggttct gcactcccgg catgttgatg tcggccaaag
     1801 ccctgctcga cgcggaccct catcctgaca gggacgagat acgtcaggcg atagccggca
     1861 atctgtgcag gtgcacaggg tacgagcaga ttgtgcaggc catcgagtca gtagcgcggc
     1921 aggccggccc gtcggaagat cggggtttgc ggaaagatca tgtggaaggc gggtgcgaat
     1981 cggatggtgg acaaggttcc tgacctcatc atcggcaacg gtgtagtcgt gacgtgtgat
     2041 tccaagtcgc aggtgatcga agacggggca gttgctgtcg caggcgaccg agtcgcggac
     2101 atcgggccta cacaggacct tgtgcagagg tatcccggcg cgaagtttga ggacgtgggc
     2161 ggacgcctgg taatgccggg gatgacttgc acacacatgc acttatacag tacattcgct
     2221 cggggcatgc agctagatgg cgagccggcg cgcactttcg ggcagatact cgagagacta
     2281 tggtggcggc tagaccgagc tcttaccgaa gaggatgtat ggatgagcgc catgatgccc
     2341 ctgatatggt gcgtgcgcaa tggaaccaca accatactgg accaccattc tgggccaaat
     2401 gccatacctg gttccctgga tgtgatcgcc gatgccgtta accgggtggg aatgcgcgca
     2461 tcgctggctt acgaagtgtc ggacagggat ggggagaaag cccgtgacca gggcatcgct
     2521 gagaacgtgc gttttatcga agcatgcaag agaaaccgtt cgccgctgct tcgggccacg
     2581 ttcgggctgc atgcctcgtt cacgttgtcc gatgagactc tcaagaaatg tgtggatgcg
     2641 ccgggcgcca gggaagccgg attccacatc cacacggccg aaggcatcga ggacctgatc
     2701 cagagcatca ggcagtacgg gcagcccgtc gtaaagcggc tagacaagat gggattgtgg
     2761 aacgataggt cgctggcagt gcactgcgtg cacatcagcc ccgaggagat cgagatacta
     2821 agggagagag acgtgaacgt ggtccacaac cccgaatcca acatggggaa tgctgttggc
     2881 gcggcgccgg cactggagat gatcaagcgc ggggtgcgag tgggtctcgg caccgacggc
     2941 tacacgtacg acatgttcga aagcatcaag gtgggcaatc tgatccacaa gcacaaggag
     3001 gccaaccctc aggctgcgtg ggcagagatc ccgctgatgg cgttcgagaa caactccaag
     3061 atactggcga aatactggga cgtgcccctc ggtgttttga ccccaggtgc gtatgccgat
     3121 gtgattgtgg tcaactactg ggctcctacg ccaattacca gcagcaactg gtacagccat
     3181 gtgctgttcg gggtatccgg cggtatggtc gacagcacga tggttggagg caagttcttg
     3241 atgcgccggg gcgagttgct caccgtgaac gagcaggaga tggccgccaa gtcgcgggag
     3301 atggcacgtg gggtgtggga acgcgcttga cagcgccacc acgccggcac accgatgtga
     3361 cgatgatgga cgggtagacg ggaacccgtc cattgttgtt gtacgcgcaa gatctgtagc
     3421 cgttggtagg caacatatat gtgctatgcg ctgcgtgttc gctgagaaag gccggcctga
     3481 atggcctttt gcaccgccgc ctgaaatggc aaagcgaccg ggtgggacct gggctacccg
     3541 acacacaggg cgcggtcggc ctctgcctac ctgtcggctt tggatggctg atatctaagg
     3601 tgggtctggc actagcctgt aacgatactg cccggtctgt acatcatttt gttcagagag
     3661 tgcccggttt tgaccgggag cagacaaaac cagggatata ttggtgcttc atggagagtg
     3721 tacggtcgcc taagtccttt tcgggcatat tcgagggata gaggcacatg gccagataat
     3781 accagactcc tccgcctgcg gattcgacga cggcgtgatt acagaaaaag gaggagccgt
     3841 tgccggcgcg aattcggaca attatcgcct gccaaaccag gcaggatctg aacaggatgc
     3901 tcgaaaagcc agttcaaaaa tggacaggat gccccagaac cccgttaggg accgccacag
     3961 gggagctccg cactcagtaa tatccataga gattgggagg acagtgaccc tccgcctcca
     4021 gaacactgca agtcttcccc agcgtaatgg ctgttgactc ccatcatatg gaacggtata
     4081 cttgacatgg aagaggccta tcaataaagg aggacttatc catgaacaga cataatcagg
     4141 ataccagtcc tggcggtgtc ccgctgccgc gctcgggcgg agtggccgtc tcttcggatc
     4201 ccacgcctat cggtgtgaga gaggccgttg ctgtgccggc cggtgctggt ctgtcgtctc
     4261 tgattcgcgg caaggctgag aacggtgttg taatgc
//
LOCUS       DAOTAC010000226         4293 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_16727_length_4293_cov_3.899009, whole genome shotgun sequence.
ACCESSION   DAOTAC010000226 DAOTAC010000000
VERSION     DAOTAC010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4293
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16727_length_4293_cov_3.899009"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1331)
                     /locus_tag="PLM74_09270"
     CDS             complement(<1..1331)
                     /locus_tag="PLM74_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009166023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HON43299.1"
                     /translation="MQGLIGLLKAAGSLMSLQPLLLITAGIIVGILAGAMPGVSPSMG
                     VALMVPFTYAMDPSLALIYLAAIYLAANYGGSITAVTINTPGTPSAVVTAFDGYPLAR
                     KGRAGEALGVSLVASFVGGAIGIIVLMLFSAPLAKAALKFWPAEYFSLAVMGLSSIAS
                     MGGQNALKSAISAVLGLLICTIGMDPILGVRRFTFGNVHLFDGFEFIPVLIGLLALSE
                     VFVEVEKFDFRAESVGDSAKAKWPSFVYYWKLRMSMLRASVIGTIVGIFPGAGATIAT
                     FISYDMEKRFSSTPEEFGQGMPEGVAAAEASNSASVGGALVPMLTLGIPGSATAAVLM
                     GALMIHNLQPGPELFTKSPELIHKLFASLLIGNVIMLILGLGGSRLWIKVTKVKKQIL
                     FPLIFAFAMIGSYAVNSSMWDVGVCLGFGVIGWLLKRHGYSLPPLVLGMVLG"
     gene            complement(1351..1920)
                     /locus_tag="PLM74_09275"
     CDS             complement(1351..1920)
                     /locus_tag="PLM74_09275"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HON43300.1"
                     /translation="MESFSSWVAGSGQVILPLLGACIVLLAWALARRGSMRKYAGRIA
                     LDAALLAVGLVAYILTYGFPSMSFAGVDAETVPRLWAGLLVALAMARLIRVFRGKDSP
                     DPASGRLDKVFLLIALLVLKIVGITFVGYYISAGVFVFVCGLLLGYRNLPGLALVAGG
                     WVAFSYFVFYRLLSVPLPTGLLLAAVLGR"
     gene            complement(1945..2919)
                     /locus_tag="PLM74_09280"
     CDS             complement(1945..2919)
                     /locus_tag="PLM74_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HON43301.1"
                     /translation="MNRNRVRMLSVALAALLVLSFALGVAAKDEFPAKPIRIINYVAP
                     GGVMDVTTRKFVDVAAKYTKAVFVVENRTGAGGLIGMDYVLGQPADGYTVFAPTTSVV
                     IQVVSTSSNVDKYIWGFEWASMLMRDPECIISNIKLPINTWPDIVKDAKAKGGRQIWV
                     GPSTGGNDHLMAMKVWDKAGISAMWIPFESGPAAMVALMGGQGVAYVGNPVDTAGRPD
                     LKVCAVSSATRLKQYPDAPTFRELGLAGLDDESMWRGFAVKKGTPPEAVKWLQDLVEK
                     VAKDPEWKNFFEEQAIEVVAYRTDEFTKHVKTNLEDAQRYFKQFGIIK"
     gene            complement(2974..3894)
                     /locus_tag="PLM74_09285"
     CDS             complement(2974..3894)
                     /locus_tag="PLM74_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HON43302.1"
                     /translation="MDRKAVIDIGTNSIKLYVAERASDGSLTSVVDKNNIARLGEGMG
                     DTGILQPEAIARNAQAVAEFAGLARDSGAQEIVAVGTMALRTAKNAADFVSVVQNLSG
                     VDVRVIPGEEEAGLAYLAVLSGIGASAGRLAVCDVGGGSTEFIFGGDAGVDRRFSINL
                     GAVKVSDGYLASDPPSPEQVDLAMSFVLDALRANRVDAARPVDRLIGIGGTVTSMGSV
                     KLKMAEYDPDLIQGAALSRDDVDELIRLFCSVSLDDRRRIVGLQPKRAEVIIGGACIV
                     RCIMECLDVPELTMSDRGLRHGLMFQLFQR"
     gene            complement(3895..>4293)
                     /locus_tag="PLM74_09290"
     CDS             complement(3895..>4293)
                     /locus_tag="PLM74_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43303.1"
                     /translation="AVNDDGLEQWRPIMKEQFPLGVDDLVRLLGVLQVAAPSDLKESY
                     TYDEFIEEIVKPHPDLAAVNVSKRRYGLTVNGCTVEFAHTEFNSVPLQTICVEHADPA
                     LVIATVRQLGLESYANINYIAAMKRVVGMN"
BASE COUNT          953 a         1379 c         1105 g          856 t
ORIGIN      
        1 cccaatacca tacccagtac cagcgggggc aacgagtatc catgtctctt cagtagccag
       61 ccaatcacac caaatccaag gcataccccg acatcccaca tgctcgagtt cacagcgtaa
      121 ctgccgatca tggcgaacgc gaatatgagc gggaatagta tctgcttctt gactttagtg
      181 accttgatcc acagccggct gcctccaagg ccgaggatca gcattatcac gttgccaatg
      241 agaagagaag cgaagagttt gtgtatgagc tcgggactct tcgtgaacag ctcgggccct
      301 ggctgcaggt tgtgtatcat caacgcgccc atgagcaccg ccgcagttgc gctgccaggt
      361 atcccgaggg tgagcatagg cactagagcg cccccaaccg acgcgctgtt cgaagcctcg
      421 gctgccgcaa ccccctcagg cattccctgc ccgaattcct ccggcgtgct gctgaacctc
      481 ttctccatgt cataactgat gaaggtggct attgtggcgc cggcgcccgg gaatatgcct
      541 acgatagtgc cgatcaccga tgcccgtaac atcgacatcc gcagcttcca gtagtagacg
      601 aaggagggcc atttggcttt cgcggaatcg cctaccgact cggcccggaa atcgaacttc
      661 tccacctcaa cgaatacctc gctgagcgcc agcaacccta tgagcacagg gatgaactca
      721 aacccatcga ataggtgcac gttgccgaat gtgaatctcc tgacgccaag aatgggatcc
      781 atgccgattg tacatataag gaggcccaga actgccgaga tcgcgctttt caaggcattc
      841 tgcccgccca tgcttgcaat gctcgacagg cccattactg ccaaggagaa atactcggca
      901 ggccagaact tcaacgccgc tttggcaagg ggcgccgaaa acagcatcag cacgataatg
      961 cctatagcac cgcccacgaa agacgccaca agcgacaccc cgagggcttc acccgccctg
     1021 ccctttctgg ccagcggata tccgtcaaac gcagtcacga ccgccgacgg tgtgcccgga
     1081 gtgtttatcg taaccgccgt aatcgaaccg ccgtagttag cagcgagata aatcgccgct
     1141 aggtatatta gcgccagcga cggatccatt gcataggtga acggaaccat cagggcgacc
     1201 cccatcgacg gcgaaacccc gggcatagca ccggccaaga tgcccacgat gatcccggca
     1261 gttatcagta agaggggttg cagactcata agcgagcccg ctgccttaag caatccaatc
     1321 aagccctgca cgatatctcg tcctcctgcg tcatcttccc agtacggccg cgagcagcag
     1381 tcccgtaggt aatggaacgg atagcagcct atagaatacg aagtacgaga aggctaccca
     1441 tccgcctgca accagcgcca atcccggaag atttcgatag cccaacagta gtccgcagac
     1501 gaacacgaat actccggcgg agatgtagta ccccacgaac gttatgccga cgattttcag
     1561 gactagcagg gcaatgagca agaatacctt gtctagcctg ccggatgcgg ggtcaggcga
     1621 atccttccct ctgaacactc gaatgagcct ggccatcgct aacgccacta ggagaccagc
     1681 ccacaacctc ggcaccgttt cggcgtctac gcctgcgaaa ctcatactag ggaagccata
     1741 ggtcagaatg taggccacaa gacccacagc cagcaacgcg gcatccagcg cgatccttcc
     1801 cgcgtatttc ctcatggaac ccctgcgggc gagcgcccag gccagtaaca cgatacatgc
     1861 tccaagaagg ggcaggatga cctgccccga cccagctacc caagaggaga acgactccat
     1921 agccattcct ccgatccgat aggactactt gattatcccg aactgcttga agtacctctg
     1981 ggcgtcttcc aggtttgtct taacatgctt ggtgaattcg tcagtcctgt atgccacaac
     2041 ctcaatggcc tgctcctcaa agaagttctt ccactcggga tccttggcca ccttctctac
     2101 caggtcctgc agccacttga ccgcctcagg gggcgtgcct ttcttgaccg cgaatcccct
     2161 ccacatggat tcgtcgtcca ggccggcaag ccccagttcc cggaacgtgg gggcatcagg
     2221 atactgcttg agcctcgtgg ccgacgatac tgcgcagacc ttcaggtcgg gccggcccgc
     2281 agtgtcgact gggttcccta cataggcgac gccctgcccg cccatgagcg ccaccatcgc
     2341 agccgggcca ctctcaaacg ggatccacat cgcggagatc cccgccttgt cccatacttt
     2401 catggccatc aggtggtcgt tgccgccggt ggatgggcca acccagatct gcctgcctcc
     2461 cttggccttt gcgtccttga caatgtcggg ccaggtgttg atgggtagct tgatgttgga
     2521 gattatgcac tccgggtccc tcatcagcat ggacgcccac tcgaatcccc atatgtactt
     2581 gtcgacgttg ctgctggtgg acacgacctg gatgactact gacgttgtgg gggcaaagac
     2641 tgtgtagccg tcggccggct gaccgagcac atagtccata ccgatcaggc cgcccgcccc
     2701 ggtgcggttc tccaccacga acacggcctt cgtgtacttg gccgccacgt ccacgaactt
     2761 cctagtagta acgtccatga ctccgccagg cgcaacgtaa ttgatgatcc taatgggctt
     2821 cgcggggaac tcgtctttgg cagccacgcc cagtgcgaaa gacagaacga gcagggcagc
     2881 caatgcgacg gacagcatgc gaacgcggtt tctgttcatc tagctaacct cccatgtcat
     2941 cttctctggc tcccattgat tgaaagccga ttgctagcgc tgaaacagct ggaacatgag
     3001 gccgtgcctc aggcctcgat cgctcatggt aagctcaggg acatccaaac actccatgat
     3061 gcacctgact atgcaggcgc cgccgatgat cacttcggcg cgcttgggct gcagtcctac
     3121 gatcctccgc cggtcatcaa gcgacaccga acagaatagc cggatcagct catccacgtc
     3181 atccctggaa agggcagcgc cctgtatcag gtcgggatcg tactcagcca tctttagctt
     3241 gactgaaccc atggatgtta cggttccacc gatgccgatc agcctgtcaa ctggccgcgc
     3301 ggcgtcgacg cggttcgctc ggagcgcatc aagcacaaac gacatcgcca ggtccacctg
     3361 ctctgggcta ggtggatcgg acgcaagata cccatcggag accttgaccg ctcccaggtt
     3421 gatactgaac ctgcgatcga cgccggcgtc ccctccgaaa atgaactccg tgctcccacc
     3481 gccgacatcg cacacagcca gccgtccggc ggaagcgcca atgcctgaca gaactgcgag
     3541 atacgcaagt cccgcctctt cctccccagg gatgacccgt acgtcaacgc ctgaaaggtt
     3601 ctgtaccacc gagacaaagt cggctgcgtt ctttgcggtt ctcagggcca tggtgcccac
     3661 agcgacgatc tcctgcgctc cgctatcccg ggccaatccg gcgaactcgg ccaccgcctg
     3721 cgcgttgcgg gcgatcgcct ccggttgcag aatgcctgtg tcgcccatgc cctcgcccag
     3781 ccgagctatg ttgttcttgt ctacgacgct cgtgagactg ccgtcggatg cgcgttcagc
     3841 gacatacagc ttgatcgaat tcgttccgat atcgattact gcttttctgt ccatttagtt
     3901 catccccaca acgcgcttca tggcggcaat gtagtttatg ttcgcataag actccagccc
     3961 cagctggcgc acggttgcga tcacgagtgc cgggtccgca tgttcaacac atatcgtctg
     4021 caatggtacg gagttgaact cggtatgagc aaactcgacg gtgcatccat tcacagtcaa
     4081 cccatatctc cgcttactca cgttcaccgc ggcaaggtcc ggatgaggtt tcactatctc
     4141 ttcaatgaac tcgtcgtaag tgtacgactc tttgaggtca gacggagctg caacttgcag
     4201 aacgcccagc agtctcacaa ggtcgtccac acccaatggg aactgctcct tcatgatcgg
     4261 acgccactgc tccaagccat cgtcattcac ggc
//
LOCUS       DAOTAC010000227         4261 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_16871_length_4261_cov_4.830956, whole genome shotgun sequence.
ACCESSION   DAOTAC010000227 DAOTAC010000000
VERSION     DAOTAC010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4261
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16871_length_4261_cov_4.830956"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2777)
                     /locus_tag="PLM74_09295"
     CDS             complement(<1..2777)
                     /locus_tag="PLM74_09295"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucodextranase DOMON-like domain-containing
                     protein"
                     /protein_id="HON43304.1"
                     /translation="MGGRTRLALMLCITVTTALVVFGPCEAATASDAGNPLYVTLIWH
                     NHQPFYKNTATGMYMMPWVRMHAVKDYYDMAAMLKDYPNVHVTFNLVPSLIKQLDEYS
                     AGAKDIQLILTEKPAHELTQDDKDFILRRFFDANWDNIIKRYPRYWELLQLRGTSVDD
                     ECIARAMANYTEQDFRDLQVWFNLAWLDPDFIEDDPAMKGFVQKARGFTEADKQLVVA
                     KHAQIVREVIPLYREMQKTGQIEVTTTPFYHPIMPLLYDTDLARVASPKLDLPAERFA
                     YPEDVEAQLGLAVDSHKSHFGAAPRGLWPSEQAVGQKIVDIVSSAGFDWMVSSEGVLA
                     RSLGVALRDGAGNVAKPDLLYRPYIVEEDGKQVTILFRDIVLSDKIGFSYSGMTGRAA
                     AHDLVSYLKKVKADLAGVAGPHVVTIALDGENCWEYYANDGKEFLHTLYEALNADTDF
                     RAVTVEEYLAVNPATERIPVLHTGSWISDNLETWIGEAEENRAWDYLTKARRALAEAT
                     AGGEVSCGEACEAACGGACGAACEPPAGQLAQAWDEMFAAEGSDWLWWYGDDQSSGND
                     EAFDELFRVHLQNVYNYLGLPVPGYLYLPIIPKQPAVAEDKITGIMKDAVMDGMVGWG
                     EWEKASFYRGVPLEGSVTVFASLRVGVDAENLYVRLTAEGGFEALYGQDTQMAVYIST
                     PRRGEVNALPRWSEEGMDATVLGFPLGAEVIVDFANLSRRGRAGARLGFAAGDGTWVD
                     AGELAAASVGNALEIAVPFDTLGIRGGDAAVLAAVATRGAKNVDVIPSGGPGEFKAPV
                     VSIGKRLFLLEDAQGDDHGPGGYTYPTNAVFTPGCFDMVSFECREGDDEVTFNVKLAS
                     EITNPWNSGIGLSVQTIDIYIDTDHKPGSGLTEALGGRRVEFEPECAWEYAIWVEGWN
                     QKVFAADGSEVG"
     gene            complement(3029..3379)
                     /locus_tag="PLM74_09300"
     CDS             complement(3029..3379)
                     /locus_tag="PLM74_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HON43305.1"
                     /translation="MDRIIAYCGLVCDECPAYLATQADDDQARTKIAAEWSEGLGATM
                     KPEDINCDGCLGAGGRKVGYFSMCEIRACAVERSVENCAHCSDYACESLKGFLKGAPA
                     AQALLDGMREARRG"
     gene            complement(3473..>4261)
                     /locus_tag="PLM74_09305"
     CDS             complement(3473..>4261)
                     /locus_tag="PLM74_09305"
                     /inference="COORDINATES: protein motif:HMM:NF020034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HON43306.1"
                     /translation="TPTDGWLEGLRVDSTFRRHGVAKAVAHRLLEEAKDAGATSLRFA
                     TARDNTESIALNEALGFVRTGGFRYFYCDPEPLPAAVQAARARADELAASPPNARVTE
                     IEVKRLHSPGPDDPFTLAVQDSATVAGARGMLPSGFVFYPASAGHIGKMANRGCAFEA
                     HDGDGRRAVLLMDASSEMWPGAGEFVISVLEGDHALCWAVLSHAFDEMSKHGMRSVSG
                     CVPCGGMVASMMTEIGFEMWQEDVPPDMPTVLLYEYPSELLGKI"
BASE COUNT          849 a         1444 c         1117 g          851 t
ORIGIN      
        1 ccgacctcgg aaccatccgc cgcaaacacc ttctggttcc atccttccac ccagatggcg
       61 tactcccacg cgcactctgg ttcgaactcg acccttctgc cacccagggc ctccgtcagc
      121 cctgacccgg gcttgtggtc ggtatcgata tagatgtcta tcgtctgcac cgaaagacca
      181 atgccggaat tccacggatt cgtgatctcc gatgccagct tgacgttgaa cgtgacctca
      241 tcatcgccct cccggcactc aaacgacacc atgtcgaaac agcccggcgt aaacactgca
      301 ttcgtcgggt aggtgtagcc gcccgggccg tgatcatcac cttgcgcatc ctcaagcagg
      361 aagaggcgct tgccgatgct cactacaggc gctttgaact cgcccggccc gccggatggg
      421 atcacgtcca cattcttcgc tccgcgtgtt gccaccgcgg cgagcactgc ggcatcgccg
      481 ccccgtatgc caagggtatc gaaaggcact gcgatttcca gcgcgttgcc aaccgacgca
      541 gccgcaagct cgcctgcgtc cacccacgtg ccgtcgcccg ccgcaaagcc caagcgcgcc
      601 ccggcgcgcc cgcgccgcga caggttggca aaatccacta tgacctcggc ccccagtggg
      661 aacccgagca cagtggcgtc catcccctcc tcactccatc tgggcaaggc attgacttct
      721 cctcttcgcg gggtgcttat gtagacggcc atctgcgtgt cctgcccgta aagcgcctcg
      781 aatccgccct cggccgtcag gcgcacgtac agattctcag catctactcc gactcgcaga
      841 gaagcgaaga ctgtgacact gccttccagg gggacgccgc gatagaatga tgccttctcc
      901 cactcgcccc aacccaccat gccgtccata acggcgtctt tcatgatccc ggttatcttg
      961 tcttccgcca ccgccggctg cttcggaatg atgggcagat agaggtatcc cggaacgggc
     1021 aggcccaggt agttgtagac gttctgaaga tgtaccctga acagctcgtc gaaggcctca
     1081 tcgtttccgg acgactggtc gtcgccgtac caccacaacc agtcggagcc ttcggcggcg
     1141 aacatctcgt cccacgcctg cgccagctgg ccggcgggcg gctcacaggc ggcgccacat
     1201 gccccgccac atgctgcctc gcacgcctcg ccgcacgaca cctcgccacc ggctgtggcc
     1261 tccgcgagcg ccctgcgcgc cttggtaagg taatcccagg cgcggttctc ttccgcctcg
     1321 ccgatccatg tttcgagatt gtccgagatc cacgaaccgg tgtgaagcac cggaatgcgc
     1381 tccgtagcgg ggttgacggc cagatactcc tcaaccgtca ccgcccggaa gtcggtatcg
     1441 gcattcaagg cctcgtacag cgtgtgcaaa aactccttgc cgtcgttggc gtagtactcc
     1501 cagcagttct caccatcgag agcgattgtc acaacatgcg gcccagcgac tcccgcgagg
     1561 tctgccttga ccttcttcag gtagctcacc aggtcgtgcg ctgccgcccg cccggtcatg
     1621 ccggagtaac tgaagcctat cttgtcagag agcacgatat cgcggaacaa gatagtcacc
     1681 tgcttaccgt cttcctctac tatgtacgga cgatatagca ggtcgggctt cgccacgttt
     1741 ccggccccat cccgcaatgc caccccgagc gacctcgcca gaactccctc gctcgacacc
     1801 atccagtcga atccggcact cgacacgatg tcgactatct tctgccccac tgcctgctcc
     1861 gatggccaca gcccgcgcgg cgcagctcca aagtgcgact tgtgggaatc gaccgcgagg
     1921 cccagctgag cttcaacgtc ctccgggtac gcgaaccgct ccgcaggcag atccaatttg
     1981 gggctagcta ctctcgcaag gtcagtgtcg tagagcagcg gcatgatggg gtggtaaaac
     2041 ggcgtggtcg tgacctcgat ttgcccggtc ttctgcatct ccctgtagag cggaatcacc
     2101 tcgcggacga tctgggcgtg cttcgcgacc acaagctgct tatcggcctc ggtgaacccc
     2161 cgtgccttct gcacaaatcc cttcatggcc ggatcgtctt cgatgaagtc ggggtcgagc
     2221 catgccaggt tgaaccatac ctgcagatcc cgaaagtcct gctcagtgta gttcgccatt
     2281 gcccgggcaa tacactcgtc atccactgat gtgccccgca gctggagcaa ctcccagtag
     2341 cgagggtatc tcttgatgat gttgtcccaa ttggcatcga agaaacgcct gagtatgaag
     2401 tccttgtcgt cctgggttaa ttcgtgagca ggcttctccg tcaggatcag ctgtatgtct
     2461 ttcgcgccgg cggaatactc gtccagttgc ttgatcaagg atggaacaag gttgaacgta
     2521 acatgcacat tgggatagtc tttgagcatc gcagccatat cgtagtagtc cttcacagcg
     2581 tgcatgcgca cccagggcat catgtacatc cctgtagcgg tattcttgta gaagggctgg
     2641 tgattatgcc agataagagt cacatagagc ggattgcccg cgtcgctcgc agtcgcagcc
     2701 tcacaaggcc cgaaaacaac cagcgccgtg gttacggtga tacacaacat gagcgcaagt
     2761 cgcgtacgac cccccatgat ccatgcctcc ccgcaatgat ggtattgctg ccaacagacc
     2821 acattcgcag catattagtg ttcgcccaat tgggcgaaac atcctttgtt gcttgggaga
     2881 cgtagaataa ccgccgtgat ggccgggcgc gcaggtacac aaacagagca ggcgccaggc
     2941 catcacgcct gattagtcgg ctggttagcg gaccatctga ttagtcgtct ggttgactgc
     3001 cagatcgctc gactacctcg ttgtgtggct agccccgtcg agcctccctc atgccatcga
     3061 gcagcgcctg agccgccgga gcgcccttta gaaacccctt gagcgactcg catgcatagt
     3121 cggagcagtg cgcgcagttt tcgacagacc gctcaaccgc acacgcccgg atctcgcaca
     3181 tgctgaagta ccccactttc cgaccgcccg cgcccaggca accatcgcag ttgatgtcct
     3241 ccggcttcat agtggccccc aagccctccg accattctgc cgcaatcttg gtcctcgcct
     3301 gatcgtcatc ggcctgagtg gctaagtatg ccgggcactc gtcgcacacg agcccgcagt
     3361 aagcgatgat cctgtccatg tcgccacaac ccccttatgc tctgtagaac acatgttcgg
     3421 cggcaatcgt agaattcctc ccaggagcgc catctatttt gtgcaatgat gcctagatct
     3481 tccctagcag ctcagacggg tattcgtaca acagaaccgt aggcatgtca ggtggtacgt
     3541 cttcctgcca catctcaaac ccaatctcgg tcatcatcga cgcgaccatc ccaccgcacg
     3601 gtacacagcc cgataccgaa cgcatcccgt gtttcgacat ctcgtcgaaa gcatgcgaca
     3661 gaacagccca gcatagcgca tggtccccct ccagcacgct gatgacgaat tcccctgccc
     3721 ctggccacat ctccgacgaa gcatccatga ggagaaccgc gcgtcggcca tcgccatcgt
     3781 gtgcttcaaa agcgcaacct ctgttcgcca ttttcccaat gtggcccgcg gacgcaggat
     3841 agaacacgaa tccagacggc agcattcctc ttgcgcccgc taccgttgcc gaatcctgaa
     3901 ccgccagcgt gaatggatcg tccgggccag gcgaatggag ccttttcacc tcaatctcgg
     3961 taacgcgagc attaggcggc gacgcggcca gctcgtccgc gcgcgccctg gcggcctgaa
     4021 cagctgcagg cagtggctca gggtcgcagt agaagtagcg aaaccctccg gttctcacaa
     4081 agcccagcgc ttcattgagc gcaatgctct cggtattatc gcgagccgtg gcgaacctga
     4141 gcgacgtcgc acctgcgtcc ttcgcctcct ccagcagtcg gtgggcaaca gcctttgcca
     4201 caccgtgcct ccgaaacgtc gaatccaccc gaagtccctc aagccacccg tcagtgggcg
     4261 t
//
LOCUS       DAOTAC010000228         4257 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_16891_length_4257_cov_3.344836, whole genome shotgun sequence.
ACCESSION   DAOTAC010000228 DAOTAC010000000
VERSION     DAOTAC010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4257
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16891_length_4257_cov_3.344836"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="PLM74_09310"
     CDS             <1..553
                     /locus_tag="PLM74_09310"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HON43307.1"
                     /translation="RWEGTIEYSFMAPQPRGVYMFGVCMWGILYGFVRTAICLAAVTL
                     MFRISLAGANLWAALLTLAASSLAFIGMGLAAAVLPLISPEKGSPATHIFQALVLLVS
                     GVYYDVSVLPAWVRPFSYVSPATYTLRAARAALLEGAGLRALAPDLIMLLVLGVVLIP
                     VGFAVFSAGERYAMRTGKLKRNG"
     gene            complement(632..3106)
                     /locus_tag="PLM74_09315"
     CDS             complement(632..3106)
                     /locus_tag="PLM74_09315"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HON43308.1"
                     /translation="MHRGFDSLGPCCSVGWNSNGVTLTTSSAQVDIKALSHSVVRVRM
                     VPQADANLRLPPSFAVVEPGWPIPEVTCEETAGHVRLSTSNITVEAQKNPLRVSFVRP
                     DGMPVAADSPAHGMGSTERGAGCYKAFAPGTHFYGFGERTGFLDKQRTAMTLWNSDVN
                     PHTPGTDSLYVSIPFFIAFSGQSAYGIYMDTAARATFNMGRPKFMPWTSEINLRPLPY
                     PEVETGEYWFGTEEKRLDYYFFAGPKIPDVVRAYSDLTGRMQLPPLWALGYHQCRFSY
                     CPERQVREVGRMMREADIPCDAIYLDIDYMDGFRVFTFNPRHFPDPAGLISDLGKDGF
                     KVVTIVDPGVKVDSQYPVYRQGRRKRVFVRRPDGKTYKGRVWPGVVGYPDFARPASRR
                     WWADNHKILFDAGVRGIWNDMNEPSNFATDTGTIDETALHGEPGAEVEHAYVHNAYGN
                     LMGQATREAFARLLPNTRPFVISRAGCGGIQRHACVWTGDNSSWWEHLLMAIPMCCNM
                     SLSGVSFVGADVGGFMDNADPELVTRWTQFGSFVPLFRSHSMIRSVPQEPYTLGEPYT
                     SICRKYIKLRYRLMPYMYTLMRQCAQDGTPVMRPMVYDFSDDSATHAMYDQFMLGPDI
                     MVAPVYLPGAVARPVYFPRGRWASLETGQIVESMGEHILVGCPLDQIPVYLREGAILP
                     WGREMSYVGQRGQAIESIDVFPSSGASGNIFSIYVDDGETLDYKDGKFGFVDISYSAR
                     EPGGLRLTITSRDECYPCTMDVGVVHILGMSATPGRVLLNEAPLSRVASRDELDRHGF
                     FCDPVTGSITLGLHVPSRHVAVDIGF"
BASE COUNT          840 a         1207 c         1304 g          906 t
ORIGIN      
        1 gcggtgggaa ggcaccatcg agtattcgtt catggctccc caacctcgag gagtctacat
       61 gttcggagtg tgcatgtggg ggatcctcta cgggtttgtg aggacagcta tctgcctagc
      121 cgcggtcact ttgatgtttc ggatatcgct tgcaggcgcc aacctgtggg cggctcttct
      181 gactctggcc gcgtcgagcc tggcgttcat aggcatgggg cttgccgccg ccgtgctgcc
      241 gctgatttcg ccggagaagg ggtcgccggc cacccatatc ttccaagcgc tggtgctact
      301 ggtgtcgggc gtctactacg atgtatcggt gctgcccgca tgggtgaggc cgttttccta
      361 tgtgtccccg gctacgtaca cactccgggc agccagggct gcgctgctcg aaggggccgg
      421 cctccgggca ctggctcccg acctgatcat gctgcttgta ctgggagtag tgctgattcc
      481 tgtggggttc gccgtcttct ctgcgggtga gcggtatgcg atgagaaccg gcaagctcaa
      541 gagaaacgga taaggccaaa gcggggcggc ctgctccgcc ccgctttggc ctcaggtgac
      601 aagtgagcag ctgacaagtg ggagatgatt gtcagaagcc tatgtctacc gcaacatgcc
      661 tggaaggcac gtgtaacccc agagtgatgc tgccggttac cgggtcacag aagaaaccgt
      721 gtctgtccaa ttcgtctcga gatgcgactc gggagagtgg tgcctcgtta agcagaacgc
      781 gtccgggcgt agcggacatt cctaggatat gtacaactcc gacgtccatg gtgcacggat
      841 agcattcgtc tcgcgaggtg atggtcagtc tcagcccacc cggctcgcga gcgctgtagg
      901 agatgtcgac aaagccgaac ttgccgtctt tgtagtcgag ggtctcgccg tcgtctacat
      961 atatggaaaa gatgttcccg gatgccccag atgaggggaa gacgtcgatt gactctatgg
     1021 cctgtccacg ctggcctacg tagctcatct cacgccccca gggtagtatg gccccttccc
     1081 gcaaatacac cgggatctgg tcgagggggc acccgacgag aatgtgttcg cccatgctct
     1141 cgacgatctg gccggtttcc aggctggccc atcgcccgcg cgggaaatag actggtcgcg
     1201 cgacggcgcc cggcaggtag actggggcga ccatgatatc cggacccagc atgaattgat
     1261 cgtacatggc gtgagttgct gagtcgtctg agaagtcgta caccatgggg cgcatcacgg
     1321 gcgtcccgtc ctgcgcgcac tggcgcatca aagtgtacat gtagggcatc aggcggtaac
     1381 ggagcttgat gtacttgcga catatagacg tgtagggctc tccgagagta tatggttcct
     1441 ggggtactga tctgatcatg ctatggctcc gaaatagggg cacaaacgag ccgaactgcg
     1501 tccatctggt cactagttca ggatcggcgt tgtccatgaa cccgcccaca tcggccccca
     1561 cgaacgatac gcccgacaag ctcatgttgc agcacattgg aatcgccatc aacagatgct
     1621 cccaccagct ggagttgtcg ccagtccaca cacaggcgtg gcgctgaatc ccgccgcagc
     1681 ctgcgcggct gattacgaac gggcgggtat taggcagcag cctggcaaaa gcctctctcg
     1741 tagcctggcc catcagattg ccgtaggcat tgtgcacgta ggcatgctcc acctcggcgc
     1801 cgggttcgcc atggagggcg gtttcgtcga tcgtgcctgt gtcggtggcg aagttggacg
     1861 gctcgttcat gtcattccat attccgcgaa cgcctgcgtc gaacaggatt ttgtggttgt
     1921 ctgcccacca tctccggctt gcaggccttg caaagtccgg ataaccgaca acgcccggcc
     1981 agacacggcc tttgtacgtc tttccgtccg gtcgtctcac aaacacgcgt ttgcgtcggc
     2041 cctgccggta gaccgggtat tgactatcaa ccttcacgcc cggatctact atggtcacca
     2101 ccttgaaacc gtctttgcca aggtcggata tgagcccggc tgggtccggg aagtgcctgg
     2161 ggttgaacgt gaatacccta aatccgtcca tgtagtcgat gtcgaggtag atggcatcgc
     2221 atggtatgtc ggcctctcgc atcatccgcc ccacttcgcg tacctggcgt tccgggcagt
     2281 agctgaaccg gcactggtgg tatccgaggg cccagagcgg cggcaactgc atgcgaccgg
     2341 tcaggtccga ataggcgcgt acgacatccg ggatcttggg ccccgcgaag aagtagtagt
     2401 cgaggcgctt ttcttctgtc ccaaaccagt attctccggt ctctacctcg ggatagggga
     2461 gtggacgcag gttgatctcg gaagtccaag gcatgaactt gggcctgccc atgttgaaag
     2521 tcgcacgtgc ggcggtgtcc atgtatatgc cgtatgctga ctggccggag aatgctatga
     2581 agaatggtat ggacacatac agcgagtcgg ttccgggtgt gtgcgggttc acgtcgctgt
     2641 tccagagagt catcgcagtt cgctgcttgt cgagaaagcc cgtgcgctcg ccgaaaccgt
     2701 agaagtgagt gccgggcgcg aatgccttat agcatccggc gccgcgctcg gttgagccca
     2761 tgccgtgggc cgggctgtcg gctgcgaccg gcatgccatc cgggcgtacg aaggaaaccc
     2821 tgagagggtt cttctgcgcc tctacggtga tgttggatgt agatagccgg acatggccgg
     2881 ctgtctcttc gcacgtgact tcaggtatgg gccaccctgg ctcgaccacg gcgaatgacg
     2941 gaggcagcct gaggtttgcg tcggcctggg gaaccattcg tacgcggacc acgctgtgtg
     3001 acagcgcttt gatgtcgacc tgcgccgacg aagtggtgag tgtgacgcca tttgagttcc
     3061 aaccgacact gcagcatggg ccaagagaat cgaaaccacg gtgcaaaaca accaacctcc
     3121 ataagcacaa gtccgtggca gccgacggct aatgggaatc ggcgccaacc gcatgaggca
     3181 aacaatgcca caattcgagg ctgtccggag aaaccctgct gcaaggctcc gccaaagtct
     3241 tgttggaaaa cacataggtc gtaggatggg ttgggaattt cacggcattt catagtgcat
     3301 tccatcgttg ccagatgaac tgcagaagtc ttggcgacaa gcgaatacag gtcgtacggc
     3361 tatgctaccc agctctccag tcgcccactt gtgcgcctca ctcatgctca tggtctgttc
     3421 ctcccccgtc gtcatgtgcg tcggaagcat gctaccacat catggctttc acgttcaacg
     3481 gcgcatcgaa tctcggcgtc ttcagcgaac ctatgccctg tgggcgaggt tattcgcggg
     3541 ccctatgacg accgacatag gttggtcgaa agacctctcc cgtaggtcat aggtcccggt
     3601 cacggaccgc tcctcgggcc gggcgaccca actgaccgcc ggtcgagcct cgcaagagcg
     3661 gcccgagtaa tcgagccttg cgggcaaggt gttcattctg tgaacaagat gtgtatcctt
     3721 accagcgata tgggtaatgc ataggaccat tacggtgggc cactggctga ggggatcagg
     3781 ctcacctcgc cggaagggct gtttagctgg ttttgacatc atggccgtga gacaaaacgg
     3841 ctggagctgg catgtcgtcg atttctgcca tcagtttcgg agatacccgc aattttcgag
     3901 tacatcgact tcggaggacc gctggacggc cttcgaacca cgagaatcgg ggggtatccg
     3961 acgggttcgg gcggcggagc aggcgggcgg gccgacacaa tatcccacta aatgggggcg
     4021 tttggtaaca gcacgcgagc gacggcatga cgtggcatgt cattaccagc gactgggttg
     4081 cgattggccc agagggcttc tttgataggg ccgattcgcc cccgggaccc ccgacccaaa
     4141 tcgatgtact cagaagttgc gtttttcgcc gaaagtactg tcaaaaagtg accgtatctt
     4201 tcggcagata cccccggggg tatatcactg agccacagct gtggacctgg cgagatt
//
LOCUS       DAOTAC010000229         4223 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_17065_length_4223_cov_4.776631, whole genome shotgun sequence.
ACCESSION   DAOTAC010000229 DAOTAC010000000
VERSION     DAOTAC010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4223
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17065_length_4223_cov_4.776631"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..698)
                     /gene="gmk"
                     /locus_tag="PLM74_09320"
     CDS             complement(51..698)
                     /gene="gmk"
                     /locus_tag="PLM74_09320"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015881336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HON43309.1"
                     /translation="MSRERVEFPRTRGNLIVISGPSGSGKNSVLARVKEGVPNLTYSV
                     SATTRAPREGELDGVHYFFLKHDEFVSRMEAGEFLETAEFCGNYYGTPRSLVERSLSA
                     GEDVIMDIEIRGADQVRAAMPDAVFIFLVPPSYAELRARIERRGSESSEAVERRLAKA
                     MEEIPAVFRYDYVVMNYDLNRAVDQIRSIILAERARVSRCDCGCFVRRFAPESPN"
     gene            complement(762..1031)
                     /locus_tag="PLM74_09325"
     CDS             complement(762..1031)
                     /locus_tag="PLM74_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010292837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HON43310.1"
                     /translation="MKLVSIGFGNIVAANRLVAIVSPESAPIKRIIREGRERGMLIDA
                     TYGRRTRAVMIADSGHVILTSVQPETVAQRVRLTQLQDAEADGRG"
     gene            complement(1049..1360)
                     /gene="mihF"
                     /locus_tag="PLM74_09330"
     CDS             complement(1049..1360)
                     /gene="mihF"
                     /locus_tag="PLM74_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007537111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor, actinobacterial type"
                     /protein_id="HON43311.1"
                     /translation="MALPELTPEQKREALKKAQEVRSKRAQVRADLKAGNMTLEGVLN
                     KAEDEVIGKMRVAYLLESLPRIGKVRSRQIMEEIGIDESRRVQGLGVRQKEALLKRLA
                     K"
     gene            complement(1415..2293)
                     /locus_tag="PLM74_09335"
     CDS             complement(1415..2293)
                     /locus_tag="PLM74_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HON43312.1"
                     /translation="MLDSMTGYGRAVCVCGSDVVTVEARSVNHRYLDVSFRMPREFAA
                     VEESARAFVSERLSRGRVDVAVSVEWHSPLRRTVVFDTHLARAYAEGLASLAADLSLT
                     GGVSVDVLTRLPDVVTIQESAADSETLWAMIRPALGEALDQLVAMRRVEGQRLESDIV
                     HRVRKIEQTLVEIVKHAPDAASAYRTRLERRVAEMLGDVRVDEARLATEMALFADRSD
                     YTEETVRFKSHIGQFRRTLDEGGARGKKLDFITQEMNRESNTIASKAQDSLISALVVE
                     IKSELEKVREQVQNVE"
     gene            complement(2463..>4223)
                     /locus_tag="PLM74_09340"
     CDS             complement(2463..>4223)
                     /locus_tag="PLM74_09340"
                     /inference="COORDINATES: protein motif:HMM:NF012892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HON43313.1"
                     /translation="RTGLGRAETVPAERRIEDPSSDPILRDLLLCAALCNDASLTREQ
                     SGQSTPPAGSGRLPARRKTRIWDVVGDPTEGALLAMAHKGGLAVMRRVRSFARVSEIP
                     FEPERRMMAVACEGPEGVFTFVKGAPQTVLELCDSRYVGRERAARDARFDREVLARNS
                     DMARGSMRVLASACARGNLIEERAGADARVHPHLTFLGLTGMMDPPRPEAVKSVERAR
                     RAGIRTIMITGDHAETAEAIAVQTGLISPDQLADGRARSSLIISGEQLDTASDQEAMR
                     MVDTAAVFARVAPAHKLRIVRALRQRGHIVAMTGDGVNDAPAIREADIGISMGLKGTD
                     VAREASSMVLGDDNYATIIAAIEEGRSIYDNIRKFIRYLLASNVGEVGSMLLTAMVGL
                     PLPLTPIQLLWMNLMTDGLPAMALAADPTDPEAMSRPPRRPDEGVFAHGLGLKIIAQG
                     AFIGLCTIACYILAGLALGHDLPTARTMAFCTLVMSQLIYVFHCRSERHSASEVGLGS
                     NRLLVAAVAVSVALQVAGVQLPVGHTYLGTRPLSLIDWALVLFLSGWSVVLTAAARAA
                     RRAARRRFHRFKVRNAKKTG"
BASE COUNT          791 a         1429 c         1154 g          849 t
ORIGIN      
        1 atccgccggc gcccacgcgc ggcgtcaggg aggccgaggt gtaggttgcg ctagttgggc
       61 gactccggtg caaacctgcg cacaaagcag ccgcagtcgc acctcgacac ccgtgcccgt
      121 tcagcgagga ttatcgacct gatctggtcg acagcccgat tcaggtcgta gttcatcact
      181 acatagtcat atctaaacac ggcgggtatc tcctccatgg ctttggccag gcggcgctcg
      241 accgcctcgg aagattccga tccccgcctc tcgatcctcg ccctgagttc ggcatacgac
      301 ggcggtacta ggaatatgaa gacggcatca ggcatggcag cacggacctg gtcggccccg
      361 cgtatctcga tgtccatgat gacgtcttcg ccagccgaca ggcttcgctc gaccaacgac
      421 cgcggagtgc cgtagtagtt gccgcagaac tccgccgtct ccaggaattc gcctgcctcc
      481 atcctcgaca cgaactcatc atgcttgagg aagaaatagt gtaccccgtc cagctcgccc
      541 tcgcgcggcg cccgggtagt cgcagatacg gaataggtca gattgggcac gccctccttc
      601 accctagcaa gcaccgaatt cttgcccgat cctgaaggcc cggagatcac tatcaagtta
      661 ccgcgagttc tagggaactc gacacgctcg cgtgacattt cacaaccccg tcgtttcctt
      721 ggtttcgcgc gttgcttcga tgcgcgctag gggacgcagg ctcagccgcg cccatcagct
      781 tcagcatcct gcagctgtgt gagcctgact cgctgggcaa ccgtctcagg ttgcaccgat
      841 gtcagaatca catggccgct gtcggcgatc atgacggctc tcgtccgccg accgtacgtc
      901 gcgtcgatca acattccgcg ttcacggcct tcccggatta tgcgcttgat cggcgccgat
      961 tccgggctga ctatggccac aagcctgtta gcggcgacga tattgccgaa gccaatgctg
     1021 acgagcttga tgcccacagc gaatcctcct acttggccag cctcttcaga agagcttcct
     1081 tctggcgaac gcccaggccc tggaccctgc ggctctcgtc gatgccgatc tcctccataa
     1141 tctgacggga acgtaccttg ccgatcctcg gcagcgactc cagcaggtat gcgacgcgca
     1201 tcttgccgat gacttcgtcc tccgccttgt tcaggacgcc ctcaagcgtc atgttcccgg
     1261 ccttgagatc cgccctcact tgcgccctct tgctcctgac ctcctgggcc ttcttgagcg
     1321 cctcgcgctt ctgttcgggt gtcagttctg gcaatgccat tattcgcgca cctcccataa
     1381 attgttcgcc ctgagtttgc cgtttaccgt accttcattc cacgttctgc acctgttcgc
     1441 gcactttctc cagttcggac ttgatctcga ccacaagagc cgagataagg gaatcctgag
     1501 ccttggaggc gatcgtattg gattcgcggt tcatctcttg agtgatgaaa tcgagcttct
     1561 tgcctctcgc tcccccttcg tcaagcgtgc ggcggaactg cccgatatgg ctcttgaaac
     1621 ggaccgtctc ctctgtgtaa tcgctcctgt cggcgaacag agccatttca gtcgcgagcc
     1681 tggcctcgtc gacacgcaca tcccccagca tctcagctac ccgacgctca agacgcgttc
     1741 ggtaggcgga agctgcatcg ggggcgtgtt tgacgatctc gactaatgtt tgctcaatct
     1801 tcctaactct atgcacaatg tcgctctcga gcctctgccc ctctacccga cgcatcgcga
     1861 ccagctggtc cagggcctct ccgagcgccg ggcggatcat cgcccagagc gtttcggagt
     1921 cggccgccga ctcctgaatg gtcactacat cgggcaagcg cgtcagcacg tcgactgaca
     1981 cgcctccggt aagagacaga tcggccgcga ggctggccag accctcagca taggcgcgtg
     2041 cgaggtgcgt atcgaatact accgtgcggc gaagcgggct atgccattcc actgacaccg
     2101 cgacatccac tctgccccgc gacagcctct ccgatacgaa tgcccttgca gattcctcga
     2161 ccgcggcgaa ctcacggggc attcgaaacg atacatcaag atatctgtga ttgaccgacc
     2221 tggcttcgac tgtgactacg tcgctaccgc acacgcaaac cgctcgaccg tatccggtca
     2281 tactatccaa catgagctgg caattcacct ttcattgcta accgtgcagt tcattctact
     2341 acacagatcc cagctgcatc aagtgttatg cccgctttcg gctcgaatac atggctttcc
     2401 ggtgcgtctt attccacctg cgcggcgaaa atcctgcatc tactgcggtt gttcacccac
     2461 tttcacccgg ttttctttgc atttctcact ttgaaccggt ggaaccggcg tcgcgccgcc
     2521 cggcgggccg cgcgggcggc ggcggtaaga accaccgacc accctgaaag aaacagcact
     2581 aaagcccagt cgatcaggga gagcggacgg gttcccaggt acgtgtggcc caccggcagt
     2641 tgcactccgg caacctgcag cgcgactgag acggccactg cggctaccag cagcctgttg
     2701 gagcccaggc ccacctcgga cgccgagtgc ctttcagacc ggcaatggaa tacgtagatc
     2761 agctgtgaca ttaccagcgt gcagaaggcc atggtccgtg ctgtagggag atcatggccg
     2821 agcgccaggc cggccaggat gtagcaggca atcgtgcaca gcccgatgaa agcgccctgt
     2881 gctataatct tcaggcccag cccatgagcg aacacaccct cgtccgggcg gcggggaggc
     2941 cgcgacatgg cctcgggatc cgtggggtca gcggccaggg ccattgccgg caggccgtct
     3001 gtcatcaagt tcatccacaa cagctgaatg ggcgtgagcg gtagcggaag acctaccatc
     3061 gccgtaagca gcatcgaccc tacctccccc acattactcg ccaggagata cctgatgaac
     3121 ttgcggatgt tatcatagat cgacctgcct tcctcgatgg ctgctatgat agtggcgtag
     3181 ttgtcgtcgc ccaacaccat gctggaagcc tcccgagcca catcggtgcc cttaagcccc
     3241 attgagatac cgatatcagc ctcccggatg gccggagcat cattcacccc gtcgccggtc
     3301 atggccacga tgtgaccgcg ttggcgtaga gcccgcacta tccgaagttt gtgcgcaggc
     3361 gccacccggg caaacaccgc cgcggtatcg accatgcgca tggcttcttg gtcggaggcg
     3421 gtatccagct gctcgccact gattatcagg gaggagcgcg cgcgtccgtc agcgagttgg
     3481 tcgggcgaga tcagcccggt ctgtacggct atcgcctcgg ccgtctccgc gtggtctccg
     3541 gtgatcatta tggtgcgaat gccggcgcgc cttgcccgct ccaccgattt gaccgcctcc
     3601 ggtcgcggcg ggtccatcat ccccgtaagt cctaagaatg tgagatgcgg atgcaccctg
     3661 gcgtccgcgc ccgcccgttc ctcaatgagg ttgccgcgcg cgcacgctga ggccagcacg
     3721 cgcatggacc cgcgtgccat atccgaatta cgggcgagca cctccctgtc gaaccgggcg
     3781 tcgcgcgccg ctctctcccg ccctacgtac cggctatcgc acagctccag caccgtctgg
     3841 ggcgccccct tcacaaaggt gaacacaccc tcaggccctt cgcacgccac agccatcatc
     3901 ctccgctccg gctcgaacgg aatctccgat acgcgggcaa aagagcgcac ccgccgcatc
     3961 acggccaatc cgcctttgtg agccatggcc aggagcgcgc cttcagtcgg atcgcccaca
     4021 acatcccaga tcctggtctt gcgtctggcg ggcaagcgac cagagcccgc aggaggcgta
     4081 ctctgtcccg actgctcccg ggttaggcta gcatcattgc acaaagccgc acagagcagc
     4141 aggtctcgga gaatagggtc cgacgaaggg tcctcgatcc tccgctcggc cggtacagtc
     4201 tctgcccgcc ccaacccagt cct
//
LOCUS       DAOTAC010000230         4203 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_17166_length_4203_cov_4.656702, whole genome shotgun sequence.
ACCESSION   DAOTAC010000230 DAOTAC010000000
VERSION     DAOTAC010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17166_length_4203_cov_4.656702"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="PLM74_09345"
     CDS             complement(<1..577)
                     /locus_tag="PLM74_09345"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HON43314.1"
                     /translation="MAMRADAVIMAGADNDGRLAEVSGAEYEALILINGKTMLEWVLE
                     GVLRAERVDRVIIVGPVDALSDIVESLPDAVRSRVVLVEQRGSMIDNLMAGIEQVPDS
                     KKALALSSDIPFITGEALDGFIKLCEETPAQVHYSLVPRSVNEARFPGVQRTYINMAD
                     AVVTGGNAFMVEPGIVRRNYDVIKQALGMRKK"
     gene            complement(654..1817)
                     /gene="ispE"
                     /locus_tag="PLM74_09350"
     CDS             complement(654..1817)
                     /gene="ispE"
                     /locus_tag="PLM74_09350"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HON43315.1"
                     /translation="MRARTTLLQVYVDEQGQTHGQAPQAVQVKAWAKVNLALNILARR
                     PDGYHDIETVMHRIGLADDVEVVKGGDVAVAGMSAITVKVTGVAGVDVSGVPCDLGNV
                     AFRAAQAFLECLECPGSGCPVSISIRKRIPVGAGLGGGSADAAAVLMAMNELWGEPYG
                     WRQLMAIAASIGADVPFCLMNHDLAGRDAGGSAPVNAAFAEGVGDRLTPLPGLRGVGL
                     LLVTPGYVVSTPDAYAMWDARIAEDAGSGGPATAVRQSDPAREPDRVRPVTARTARML
                     AEELSLAWSRAECADAGDDLRRMCSLMSNDFEPLIDERYGLIIQMKQLMMQAGAMGAL
                     MSGSGPTVFGVYGSPPQATEAAEVLRSLAADCGFDVDRYGFSVIDACIGDSRS"
     gene            complement(1864..3060)
                     /locus_tag="PLM74_09355"
     CDS             complement(1864..3060)
                     /locus_tag="PLM74_09355"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HON43316.1"
                     /translation="MYKRARLAALALTLLLTAGSICMTLGVSAAAGAMTPPPPTIAAK
                     SAKLVDASTGQVLYAANSEERMAPASITKIMTMLLVMEQVRAGRISLDDVVTVSTEAS
                     LMGGSQVYLKERERVPVRDLMAATAIRSANDASFALAEYVAGTADDFVRMMNQRAAEL
                     GMKNTHFTNPEGLDDPDHYTTAADIAIMSRELIAYPEVLEWTSTWIGKMRNGTYDLFN
                     TNRLIKEYEGADGLKTGHTDKAGYCLSGTAERGGVRLIAVVMGTAGEKERVAETGKLL
                     DYGFRNFERATLAEAGAKVGTLRVPTAVRPDVAAVAAEDVITLIPRGTAAEVSVEFEP
                     TEEEITAPIKKGQTLGRAVVRTAAGEELRSVRAVAESDVARANVFVRAWRSIVGFFAR
                     LLSRQK"
     gene            complement(3256..>4203)
                     /locus_tag="PLM74_09360"
     CDS             complement(3256..>4203)
                     /locus_tag="PLM74_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HON43317.1"
                     /translation="NLKKYFAIDGGFFSRTRSLLKAVDDISFTIDRGETLGLVGESGC
                     GKTTAGRTICRLYEPTSGTVTFDGVPIYSLPPSEMLPLRRRIQMIFQDPYASLNPRMT
                     VGDIVGEPIDIHGLAANKQERLEMVHELLHTVGLSPEHATRFPHEFSGGQRQRIGVAR
                     ALAVEPEFIICDEPISALDVSIQAQVINMLEELQEKRKLTYLFIAHDLAMVKHTSHRI
                     AVMYLGKIVELAGSAELYKNPQHPYTQALLSAIPIPDPDVEAQRQRIILEGDVPSPID
                     PPPGCRFRTRCKMAKKICSEQDPEFVNIGDHYVACHLVK"
BASE COUNT          809 a         1400 c         1123 g          871 t
ORIGIN      
        1 gcttcttcct catgcccaga gcctgcttga tcacgtcgta gttgcgtctt acgatcccgg
       61 gctcgaccat gaaggcgttt cccccagtta caactgcatc tgccatgttt atgtacgtcc
      121 gctgcacccc ggggaaccga gcttcattca ccgatcttgg gacaagtgag taatggactt
      181 gcgcaggcgt ctcctcgcat agcttgatga acccatcaag tgcctcacct gtgatgaaag
      241 gtatgtcgga agacagagcc agcgccttct tagaatccgg aacctgctcg atccccgcca
      301 taaggttgtc gatcatgctt ccgcgctgct caaccaggac gactctgcta cgcaccgcgt
      361 caggcaggct ctcaactata tcgctcaggg cgtccacagg cccgactatg ataacccgat
      421 ccacacgctc tgcccgaagc acgccctcaa ggacccattc gagcatggtc ttgccgttga
      481 tgaggatcag cgcctcatac tcggctcctg atacttcggc taggcgcccg tcgttgtcgg
      541 cccccgccat gatgactgca tccgctcgca ttgccatcgt cgatctcaac tccccgaaat
      601 agggattggg tcgctccgac cccgtaatca tactatttcg gcactctagc cgatcaggat
      661 cgggagtcgc cgatgcaggc gtcaatcacg ctgaaaccgt agcgatccac gtcgaacccg
      721 caatcggcag cgagagacct aagcacttcg gccgcctccg tggcctgggg aggcgagcca
      781 tacaccccaa acaccgtagg gccactaccg ctcatcaacg cgcccatggc gccggcctgc
      841 atcatcagct gtttcatctg tatgatcagc ccatagcgtt cgtcaatcag cggctcaaag
      901 tcattgctca tcagcgaaca catgcgccgc agatcatcgc cggcatcagc gcattcagcg
      961 cgagaccaag ccaaagacag ctcctcagcc agcatccgag ccgtccgtgc ggtcactggc
     1021 cgaactctgt cgggttcccg cgccgggtcg gactggcgga ctgcggttgc gggtccgccg
     1081 gacccggcgt cctcagcgat ccgggcgtcc cacatcgcat atgcatccgg cgtagatacg
     1141 acgtacccag gggtaactag cagaagcccg accccgcgta gccccggaag cggtgtgagc
     1201 cggtcgccaa ctccttctgc gaaagcggca ttcaccggcg ccgaaccgcc ggcatcacgg
     1261 cccgcgaggt cgtgattcat caggcagaac ggaacatccg cgcctatgga tgcggcgatg
     1321 gccatcagtt gtctccagcc atagggctct ccccacagct cgttcatggc cataagcacc
     1381 gccgctgcat ctgcgctccc acccccgagc cctgctccca ccggtatgcg ctttcggatg
     1441 ctgatactca ccgggcagcc actcccaggg cactccaggc attcgagaaa ggcctgagct
     1501 gctcggaatg cgacattgcc cagatcacac ggcacgccgg agacatctac gcccgcgacg
     1561 cctgtgacct ttactgtaat tgccgacatg ccggcaaccg ctacgtcgcc acccttgacc
     1621 acctcgacat cgtcagccag accaattcgg tgcatgactg tctcgatgtc atggtacccg
     1681 tccggccttc tggccagaat gttaagcgcc aggttgacct tagcccatgc cttgacctgc
     1741 acggcttgcg gcgcctgccc atgcgtctgc ccctgctcat ctacatatac ctgcaaaagc
     1801 gtcgtcctcg ccctcattgt cgcacgccgc cacgatgccg ggcagctacg ccgccgcgct
     1861 gcgctacttc tgcctgctaa gcagccgtgc gaagaaccct actatggacc gccatgctcg
     1921 cacgaacacg tttgcccgcg ccacgtccga ctcggctacc gctctcaccg agcgcaattc
     1981 ctcgccggcg gcagtgcgta ccacagcgcg cccaagcgtc tgtcctttct ttatcggggc
     2041 cgtgatttcc tcctcggtag gctcgaactc tacgctcact tcagcggcag tccccctcgg
     2101 gatcagcgtg atgacatctt ccgcggcaac tgccgccaca tcaggccgca ctgcggtcgg
     2161 gacccgcagc gtccctactt tcgctcctgc ctcggcaagc gtggctcgtt cgaagtttcg
     2221 gaacccgtaa tccaacagct tcccggtttc tgcgacacgt tctttttcgc ccgcagtgcc
     2281 cattacgacc gcgatcaggc gcacaccgcc gcgctctgca gtgcctgaca agcagtatcc
     2341 cgccttgtcg gtgtgaccgg tcttcaagcc atctgcgccc tcatactctt tgatgagccg
     2401 gttcgtattg aacagatcgt acgtgccgtt tcgcatcttc cctatccatg tactggtcca
     2461 ctccagcacc tcggggtatg caataagctc tcgcgacatg atggcaatgt cggccgctgt
     2521 ggtgtagtga tccgggtcgt ccaggccctc agggttggtg aagtgggtgt tcttcatccc
     2581 gagctccgcc gcgcgctggt tcatcatcct cacgaagtcg tcggctgtcc cggccacgta
     2641 ttcagccagc gcgaaacttg cgtcgttggc agaccttatg gcagtagcgg ccatgagatc
     2701 acgtaccggc acgcgctccc gttccttaag gtagacctgc gaccctccca tcaacgatgc
     2761 ctcggtgctc acagtgacca cgtcgtcaag ggatatccta ccggcccgta cctgctccat
     2821 gacgagcagc attgtcataa tcttggtgat ggacgcaggc gccatgcgtt cctcagagtt
     2881 ggccgcgtaa aggacctggc cagtggaagc gtctactagc ttggcgctct tggcggcgat
     2941 cgtgggaggc gggggcgtca tggcgcctgc cgcagcgctg acgcccagcg tcatgcagat
     3001 cgatcccgca gtcagcaata gagtcaatgc cagagcggca agacgggctc gcttgtacaa
     3061 tggtcatctc tcccgaactc aacatagact acctagattc ttcccgctgt gcgataacat
     3121 gttgcatatc agcgcacaag caagcccgac ggagcgccga gccgatacac gctctccgcc
     3181 gggctagcgc attccaagtg acgtgcccag ccggcaatcc acttagccaa ctacccactg
     3241 ccaactacat actgcctatt tcacgagatg gcacgcaaca tagtggtcac ctatgttgac
     3301 gaactcggga tcctgctcgc tgcaaatctt cttggccatc ttgcacctgg ttcggaaacg
     3361 gcagcctggc ggcggatcta tggggctggg aacgtctccc tcgagaatga tcctctgccg
     3421 ctgggcctcc acatcagggt cgggaatagg gattgccgac agaagcgcct gcgtgtacgg
     3481 gtgttgcggg ttcttgtata gctctgcgct ccctgccaac tctacgatct tgcccaggta
     3541 cattactgca atccggtgac tagtatgctt gaccatagcc aggtcgtgcg ctatgaagag
     3601 gtaggtgagc ttgcgcttct cctgcaactc ctccagcatg ttgataacct gggcctggat
     3661 agagacgtcc agcgccgaaa tgggctcatc gcatatgatg aactccggct ccactgctaa
     3721 agccctggcc acaccgattc tttgccgctg cccgccgcta aattcgtgcg ggaacctggt
     3781 cgcatgctcc gggctcaagc ccaccgtgtg caagagttcg tgtaccatct ccagtcgctc
     3841 ctgcttgttc gccgcgagtc cgtggatatc aatgggctcg ccgacgatgt cgcccacagt
     3901 catgcgcggg ttcaaagacg catacgggtc ctggaagatc atctgtatcc tccgcctaag
     3961 gggaagcatc tcagatggcg ggagggagta tatcggcaca ccgtcaaacg tgacagtccc
     4021 ggacgtgggc tcatacagac ggcaaatcgt cctgccggcc gtggtcttgc cacagcctga
     4081 ttcacccaca aggcccagcg tttcgccgcg gtcgatagtg aagctgatat cgtcgacggc
     4141 cttgaggaga ctgcgtgtgc ggctgaagaa ccctccgtct atggcaaagt acttcttcag
     4201 gtt
//
LOCUS       DAOTAC010000231         4192 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_17217_length_4192_cov_4.003868, whole genome shotgun sequence.
ACCESSION   DAOTAC010000231 DAOTAC010000000
VERSION     DAOTAC010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17217_length_4192_cov_4.003868"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            41..895
                     /locus_tag="PLM74_09365"
     CDS             41..895
                     /locus_tag="PLM74_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HON43318.1"
                     /translation="MNRFFHGSRGDSPAKRLLIHIVLICACVVAIYPFLRVVSISLRP
                     GSALFSTDLSLIPEGATLNNYRALIMEKDFTIWLWNSLLVSIATVIVGVLLAAAAAYA
                     FSRWSFPGRRQGLLFLLTTQMLPPGMLLLPIYVMVVRLKLFNTYTGIILSYSAGSVPF
                     SIWLLKGYYDTIPRELEEAAMVDGAGPLMAFYRVIVPLSTPALAIAGLFSFMTAWSEF
                     MMARVMLQKAGMFTWPLGFQKLMDQFSTQWNMFAAASVLVAIPAMLLFLYSSKWLISG
                     LTLGGIKE"
     gene            980..1852
                     /locus_tag="PLM74_09370"
     CDS             980..1852
                     /locus_tag="PLM74_09370"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HON43319.1"
                     /translation="MNGANPNETMKLLYERASCRSFKEEPIPDETLDAVLMAATCAAT
                     GGNLQPYSVIRVLDAQVRQRLMALCDDQPFIGKAPVNLVFCIDFHRLERWAALENAPF
                     SARRAFRHFWISLQDVVICAQTVCTAADAMGLGAVYVGTVLECLEELKDLLKLPKGVF
                     PVVMVCLGYPKGQLTQRRKFGVDVVVHDETYHEMTDEELLRAFDEKYGGQTFEATPER
                     VDSIAQACREVHGEGYTEEFLARVKRDGHLNMAQNVFGLKYPADEMPRRNRYIMGAAR
                     NAGFEWFDQGSEEQ"
     gene            complement(2325..3098)
                     /locus_tag="PLM74_09375"
     CDS             complement(2325..3098)
                     /locus_tag="PLM74_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43320.1"
                     /translation="MSFRQHIASVVPNALVRDKRLCDLCIRIYLTLQGSRFSSIEELA
                     EAVGACRARTAKAMATLVDRGWAFYIDGENRRRKAIAPAWPVSVEAAIAALIADDKEC
                     VVFLGQWLMWDWLDVLVDALDYLDNSRPDWLRNADTGYRMELDREYRSLGVAFEFQGE
                     RHFRPTEAYPDEDAQLRQQLRDDQKVGVCLRNGIRLVEITFEDLTLKGMLKKVDGLPL
                     RHYRADGPIIRTLGQFGDSCISWMRRKRAGAYHALAGLL"
     gene            3390..3890
                     /locus_tag="PLM74_09380"
     CDS             3390..3890
                     /locus_tag="PLM74_09380"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HON43321.1"
                     /translation="MELWDVFTADRERTGKTAVRGEALGAGEYHVVVHIWLVNDEGDL
                     LIQKRAACVEWAPNVWATTGGSALAGEDSAAACARELLEETGIRADMRNAELAFTLNR
                     EDSFCDVWLIRQNVDLDHCTMQAEEVSAVGWASPEEIRSMTAQGVFWSYRYLEDLFSL
                     ISRRRT"
     gene            3974..>4192
                     /locus_tag="PLM74_09385"
     CDS             3974..>4192
                     /locus_tag="PLM74_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43322.1"
                     /translation="MPRTEVRAETDTGAEVKVRVGVKAEVGAEAGGEAGAEAGAKAMA
                     EGQIAAAISRELGVSRRHVESVVRLLDEG"
BASE COUNT          875 a         1099 c         1319 g          899 t
ORIGIN      
        1 atcttcgggc atccgggggg tttagggggg tggacgagcg atgaaccggt tcttccacgg
       61 ctcacgcggc gacagcccgg cgaaacgtct gctcatacac attgtgctca tatgcgcgtg
      121 tgtagtagcc atatacccgt tcctgcgtgt agtcagcata tctcttcgac cgggttctgc
      181 gctgttctct acagacctgt cgttgatacc tgagggcgcc accctgaaca actacagggc
      241 tctgataatg gaaaaagact tcaccatctg gctatggaac agcctattgg tgtcgatagc
      301 tacggtgata gtgggggtgc tgctggccgc agccgcggcc tatgccttct cacggtggtc
      361 attccccggc cgacgccagg gcctgctgtt cctgctcacg acccagatgc tgccgcccgg
      421 aatgcttctc cttccgatct acgtgatggt agtaaggttg aagctgttca acacatacac
      481 aggcatcatc ttgagctact cagctgggtc ggttcctttt tccatatggc tgctgaaggg
      541 ctactacgac accatacccc gggaactgga ggaggcagcg atggtggacg gagccggccc
      601 gctcatggcc ttttaccgtg tcatagtgcc gctgtctacc cccgcgctgg caatcgcagg
      661 gctgttcagt ttcatgactg cctggagcga gtttatgatg gccagagtca tgctgcagaa
      721 agccggcatg ttcacatggc cgctcggctt tcagaaactt atggaccagt tctccacgca
      781 atggaacatg ttcgcggccg cgtcggtgct tgtggctatt ccggcgatgc tgttgttcct
      841 ttattcatcc aaatggctca tatccgggct tacacttgga gggatcaagg agtagccgcc
      901 atagcgatgc gatccactcg ctcctgcccg actatgcgag atattgcctg gtgccacctg
      961 atgctggagg tgtggtgtgg tgaacggtgc taatccgaat gagacaatga agttgctgta
     1021 tgagagggcc agctgcagga gcttcaaaga ggaacccatt cctgacgaaa cgctggacgc
     1081 tgtgttgatg gctgctactt gcgcggccac aggcggcaat ctgcagccgt actctgtgat
     1141 ccgggtgctc gatgctcagg tacggcagag actgatggcg ctctgcgatg accagccctt
     1201 catcgggaag gcccctgtca acctggtgtt ctgtatcgat ttccatcgac tggagcggtg
     1261 ggcagctttg gagaacgctc cgttctcggc caggcgggct ttccgccatt tctggatttc
     1321 actccaggac gtggtcatct gtgcgcagac tgtctgtacg gccgcggatg caatggggct
     1381 tggggcagtg tacgtgggga ctgtccttga atgcctcgag gaactgaaag acctgctcaa
     1441 gctgcccaag ggtgtcttcc ccgtggtaat ggtctgcctg ggatacccca aggggcagct
     1501 tacccaacgg cggaaattcg gcgtagacgt ggtggttcac gacgagacct accacgagat
     1561 gaccgacgag gaactgctgc gggcgttcga tgagaagtat ggaggtcaaa cattcgaagc
     1621 cactcctgaa cgagtggaca gcatagctca ggcttgccgc gaggtgcatg gggagggtta
     1681 caccgaggag ttcctggccc gagtgaagcg ggatgggcat ctcaacatgg cccagaatgt
     1741 gttcggcctg aaatatccgg ccgacgagat gcccaggcgg aaccggtaca tcatgggcgc
     1801 tgctcggaat gcggggtttg aatggttcga tcagggctcc gaagagcagt gaccgggcga
     1861 gcaacggact tcgggcgccg gcgccggccg agaggcgcta ctagatcggt caaaggaagg
     1921 cgggcggcat gagccgcccg ctaccgcatt ggcattgact gagctggagc cgagacccgt
     1981 tgtgaaagag ggcccgggcg tggtaaaaaa tgtatgatca tattctgaac gcatttccgc
     2041 tgatctgttc aatttggtga caatagctgc tgcataattg aacgtatagc tgtatagggc
     2101 gtcggaggcg cggcgctagt aacatataat ggaattatgg ctgggctacg acgacagaca
     2161 agggtatatg atgtcattga acacacatgt ttccccattg cacagagcga tgtccttcag
     2221 attccttgtt cggcacgtat tatgcatcta tacgtttcgt tatgcatcac ctatcgtccg
     2281 atttcgcgac acatgccgga aaaagcgttc acgaaatgga caggtcacaa aaggccagct
     2341 agagcgtggt atgcgcctgc ccttttgcgc ctcatccagg agatgcacga atcaccaaac
     2401 tgcccaaggg tcctgataat gggcccatcg gcccggtagt gcctcagggg gagtccgtcc
     2461 accttcttga gcattccttt gagcgtcagg tcctcaaagg tgatctctac taggcggatc
     2521 ccgtttctaa ggcacactcc tactttctga tcgtctctca gttgctgtct taattgcgca
     2581 tcttcgtcag gataggcctc tgtcggcctg aagtgccgtt ctccctggaa ctcgaaggct
     2641 acacccagcg agcggtactc tcgatcgagt tccatgcggt atcctgtgtc cgcgttccgc
     2701 aaccagtcgg gccgcgagtt gtcgagatag tcgagagcat ccacaaggac atcgagccag
     2761 tcccacatga gccactgtcc caggaaaacg acacactcct tatcgtctgc aatcagcgcc
     2821 gcgattgccg cttcaacgct cacaggccac gctggagcga ttgccttgcg gcggcggttt
     2881 tcgccatcaa tatagaatgc ccatcctcga tcgaccagag tggccatggc tttagctgtg
     2941 cgtgcgcggc atgcgcctac cgcctctgcg agttcctcaa tcgagctgaa tcggctgcct
     3001 tgcagcgtca ggtaaatgcg gatgcagagg tcacaaagcc gcttgtcgcg gacgagagca
     3061 ttcgggacga cactagcgat gtgctgcctg aatgacattc ggactccacc tttcggggct
     3121 cggccccatt ttgtgcctgc agcacctaga ctactactcg cgcagacagg cctaaatcgc
     3181 ctgattgggc gatttcgcag tgcaaagaga aaatctctcg cgctcaacgt gcgcacaccg
     3241 tgggagggcg gcgtgaccgc agggtaggcc gtggaggggg cgagggggcg ccttaatcct
     3301 ggagtaggcc gcggggatac gtcgtgaatg agactgaccg ctgtgataaa atagcagcgc
     3361 agcctactgc gagtaacggg agggcactca tggagctatg ggacgttttc accgctgaca
     3421 gggagagaac cggcaagacc gcagtgcgag gcgaggcgct gggggccggc gagtaccatg
     3481 tggtcgttca catctggctc gtgaatgatg aaggggattt gctgattcag aaacgcgcgg
     3541 catgtgtcga gtgggcgccg aacgtctggg caacgactgg ggggtcggcg cttgcagggg
     3601 aggacagtgc tgctgcgtgc gcgagggaac tgcttgagga gacggggatt agggccgaca
     3661 tgcgcaacgc tgagcttgcg ttcaccttga accgggaaga ctctttctgc gatgtgtggc
     3721 ttattaggca gaacgtcgat ctggatcact gcacgatgca ggccgaggag gttagcgcgg
     3781 tggggtgggc ttcacctgag gagatccgga gtatgacggc tcagggagtg ttctggagtt
     3841 atcgatacct ggaggatctg tttagcttga ttagccgtcg gcgaacctag atagcgcgtc
     3901 ccacggggta gactggcgtt catctggcga aaaatacgtg cacagacgca cataggcgta
     3961 cataggagga cttatgccaa gaaccgaggt cagggccgag acagatacag gggcagaagt
     4021 gaaggttagg gtaggggtca aggcagaggt cggagcagag gccggaggcg aggcaggagc
     4081 ggaagccggc gcgaaagcaa tggccgaagg gcagattgcc gccgcaatca gccgtgaact
     4141 cggggtttct cgccggcacg tggagagtgt tgtgcggcta ctggatgagg gg
//
LOCUS       DAOTAC010000232         4188 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_17235_length_4188_cov_4.822163, whole genome shotgun sequence.
ACCESSION   DAOTAC010000232 DAOTAC010000000
VERSION     DAOTAC010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4188
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17235_length_4188_cov_4.822163"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     misc_binding    120..403
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            465..3587
                     /gene="ileS"
                     /locus_tag="PLM74_09390"
     CDS             465..3587
                     /gene="ileS"
                     /locus_tag="PLM74_09390"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HON43323.1"
                     /translation="MFRKVDAAMDFPRMERKILEQWNKERVFERSVAQREGAPKFVFF
                     EGPPTANGLPHPGHVLTRAMKDVVLRYKTMRGYQVPRKAGWDTHGLPVELEVEKQLGI
                     NGKPQIEQYGVRDFVNKCKESVFKYEQEWRRMTERLGFWVDMDDPYVTYHDSYIESVW
                     WAVKTIWEKGLMYRGHKVVPYCPRCGTALSSHEVAQGYRDVSDPSVFVRFRARGQENL
                     SLLAWTTTPWTLPSNVALAVSPDFEYAYVETGGEILIMAKDLVDARIAAPHSILRTVR
                     GSELIDMQYEPLYTFAIPDAPAYRVVAGDFVTLTEGTGIVHIAPAFGEDDMRVAQANG
                     LPVVQLVDTQGHFDERAGDWSGLFVKDADRLIVGDLQSRGLLYTQVEYEHSYPFCWRC
                     DTPLLYYARASWFIRMSELRDRLIANNHTINWYPEHIRDGRFGNFLENVVDWAVSRER
                     YWGTPLPIWVCQDCGHEHAVGSIAELRQMAKELPDHFELHRPYIDEAVLACPKCGGDA
                     LRVPEVMDCWFDSGSMPFAQWHYPFENHDLFNESFPADFITEAVDQTRGWFYTLLAIS
                     TLLFDRAPFSNCMVLGLVLDEQGLKMSKSRGNVADVWRVFDTKGADAVRWYLYTVNAP
                     WTPTRFYEEGVTDAMRKFMGTLWNVYAFYVLYANIDGFVPREHSVPHAGRAEIDRWLQ
                     SRLAGLVREVTRLMDSYDITAAGRRIEQFVDDLSNWYVRRCRRRYWASEMTTDKEAAY
                     LTLHETLVTIAKLIAPFTPFLAEEIYDNLEPDEGSVHLADYPVPDQEMIDPLLDSEME
                     LARQVVYLGRAARNAVNIKNRQPLSKIVVQATAEEQDKLMALADLVEDELNVRGVEFA
                     QDFGEFVSHRIKPRYDLLGPKHGKLMRAIASALAEADAAGVARDVAAGRPVVLALEGA
                     GEVSVLPEEIAVETVAREGFHVETDNGRSVALDTVISDDLRMEGFARELVNKIQATRK
                     EAGFEISDRIHAVCAGGPESMRALREHREYLMGETLSLSLAQGEPDGEYIKEWDVNGE
                     KVTVGVRRVR"
     gene            3568..>4188
                     /locus_tag="PLM74_09395"
     CDS             3568..>4188
                     /locus_tag="PLM74_09395"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HON43324.1"
                     /translation="MSDGSVDLHCHTTASDGTFTPRELVEEAQRRGLLAIAVTDHDSV
                     AGIDEATSKGAEVGVEIVPGLELSTDVDDGEVHILGYFIDREDKDLLDLLESQRESRL
                     QRVRKMLDRLSGMGVQLSMDEVQRFSDGGPLGRPHVAQALIRAGQVGSWDEAFSRYIG
                     RHAPAYVRRSKLSPHDAVRAILASGGVPVLAHPGLSGHDDMIPSLVD"
BASE COUNT          867 a         1111 c         1373 g          837 t
ORIGIN      
        1 acatcaattc catcttggct cggagaaaag cccctacaac tacttgactc agtcgatgca
       61 gtggggtgag ttgacgctct gccccgcgct gtggtagtct gagttacagt tgaaaaacga
      121 aaaaggcgat gaagggaaga gtagcgctca aggccgcttc agagagcggg gggatggtgg
      181 aaaccccgtg ccggcccggc tgcgaaaatc gcccctgagc cgcgcggcga aaggacaaaa
      241 ggccgatagg cccaagggtt ctgagtagac gtggccgcag gccctgcgtt atccgggcgt
      301 atccgatgag tggacccgga tctcgcgcga gagcgctttg ccgccgctgt gcggcagccg
      361 ggccaataag ggtggtaccg cgagcttatc cctcgcccct tgtgggcgag ggatttctgt
      421 ttgtcccgcc ttacgcgtgt cttatgagga ggtagcgtcg aacgatgttc aggaaagtgg
      481 atgcagccat ggattttccc cgaatggaga ggaagatcct tgagcagtgg aacaaggagc
      541 gggtgttcga gcggagcgtt gcccagaggg agggtgcgcc caagtttgtg ttctttgagg
      601 ggcctcctac cgctaacggg ctgccccatc cggggcatgt gcttacgcgg gccatgaagg
      661 atgtcgtgtt gcggtacaaa accatgagag gctaccaagt gccgcgtaag gccggatggg
      721 atacgcacgg ccttcctgtg gaactagaag ttgagaagca gctgggcata aacggcaagc
      781 cgcagataga gcagtacggc gtccgggatt tcgtcaacaa atgcaaggaa tcggtgttca
      841 agtatgagca ggaatggcgg cgcatgactg aacgcctagg tttctgggtg gacatggacg
      901 acccttacgt gacataccac gactcgtaca tcgagtcggt gtggtgggca gtcaagacca
      961 tctgggagaa gggcctcatg taccgggggc ataaggtagt cccgtactgc ccacgctgcg
     1021 gcacggctct gtccagccac gaggtggctc aaggttaccg cgatgtatcc gacccgtcag
     1081 ttttcgtgcg attccgcgcg cgcgggcagg agaatttgtc gctgcttgcc tggaccacta
     1141 cgccgtggac actgccgtcc aacgtggctc tagctgtgtc gcctgatttc gagtatgcct
     1201 atgtggaaac cggcggcgag atcttgatca tggctaagga tctcgtagat gccaggatcg
     1261 cagcgccgca cagcatactc cgcactgtgc gcgggtctga actcattgac atgcagtacg
     1321 agcccttgta taccttcgca atccccgacg cgccggccta ccgcgtggtg gctggcgact
     1381 ttgtcacact tactgaaggg acggggatcg tccacattgc tccggcgttc ggtgaagacg
     1441 acatgcgagt ggcgcaggca aatggcctgc ctgtggtgca acttgtggat acacaggggc
     1501 atttcgacga gcgtgccggc gactggagcg ggctgttcgt caaggacgcc gatcggctga
     1561 ttgtgggcga tcttcagagc cgcgggcttc tgtataccca ggtggagtac gaacacagct
     1621 accccttctg ctggcgctgc gacacaccgc tgctgtacta tgctcgggcg tcatggttca
     1681 tccgcatgtc ggagcttcgc gatagattga tagcaaataa ccacacgatc aactggtatc
     1741 ccgagcatat acgagatggg cggttcggca atttcctgga gaacgtggtc gattgggccg
     1801 tgagccgcga acgctactgg ggcaccccgc tgcccatctg ggtatgccag gactgtgggc
     1861 acgaacacgc cgtaggtagc attgccgagc tcaggcagat ggcgaaggag ctgccggacc
     1921 attttgagct gcacaggcca tacattgatg aggctgtgct ggcttgccct aagtgcggtg
     1981 gcgatgcgct gcgggtgccc gaggtgatgg actgttggtt cgattctggg tcgatgccgt
     2041 tcgcccagtg gcactacccg ttcgagaacc atgatctgtt caacgagtcg tttccggccg
     2101 acttcataac cgaggctgtg gatcagaccc gtggttggtt ctatacactg cttgccatct
     2161 cgaccctgct gttcgaccgc gctcctttta gcaactgcat ggtgctgggg ctggtcctgg
     2221 atgagcaggg cttgaagatg agcaagagca ggggcaacgt ggcagacgtg tggcgggtat
     2281 tcgatactaa gggcgccgat gctgtgagat ggtacctata cacagtcaat gcgccatgga
     2341 cgccgacgcg cttctacgag gaaggcgtga ctgacgccat gcgcaagttc atgggcaccc
     2401 tgtggaatgt gtacgccttt tacgtgctct atgccaacat agacggattc gtgcctcgcg
     2461 agcacagcgt gccgcacgct gggcgggcgg agatcgaccg gtggctccag tcgaggctgg
     2521 ccgggctagt tcgggaggtc acccgcctga tggacagcta cgacatcacc gcggccggtc
     2581 gcaggatcga gcagttcgtc gatgatctat ccaactggta tgtgcgaaga tgccgccggc
     2641 gttactgggc cagcgagatg acgaccgata aggaggctgc ctaccttacg ctgcatgaga
     2701 cgcttgtgac tatcgcgaaa ctgatagcgc cgttcacgcc gttccttgcc gaggagatct
     2761 atgacaacct cgaaccggac gagggtagtg tacacctagc agactatcct gtgccagatc
     2821 aggagatgat cgatccactc ctcgacagcg agatggagtt agcccgccaa gtcgtgtacc
     2881 tgggtcgggc tgcccgtaat gcggtgaaca tcaagaacag gcaacccctc tctaagatcg
     2941 tcgtgcaggc tactgcggaa gaacaggata agctgatggc cttagccgat ctggtggagg
     3001 atgagttgaa tgtacgcggc gtcgagtttg cccaggattt cggtgagttc gtgtcgcata
     3061 gaatcaagcc cagatacgac ctgctcgggc cgaagcatgg caagctgatg cgcgccatcg
     3121 cgtcggcgtt ggcagaggct gatgcggccg gcgtagcccg cgatgtcgcg gccggacgcc
     3181 cggtggtatt ggcgctggag ggtgcaggcg aggtgtcagt acttccggag gagatagcag
     3241 tagaaaccgt ggcccgcgag gggtttcacg tggagacgga caacgggcga tcggtggcac
     3301 tagacacagt catatccgac gacctcagga tggaggggtt cgcgcgcgaa ctggtcaaca
     3361 agatacaggc tacccgaaaa gaggcaggat tcgagatatc cgatcgcatc cacgcggttt
     3421 gcgcgggcgg gcctgaatcc atgcgagcct tgagagagca tcgggaatac ttgatgggtg
     3481 agacactttc actcagcctc gcccagggag agccggatgg cgagtacatc aaggaatggg
     3541 acgtcaacgg agagaaggtg actgtgggtg tcagacgggt ccgttgacct tcactgtcat
     3601 accacagcat cagacggcac cttcaccccg agggagctgg tagaggaggc ccagcgccgc
     3661 ggcctcctcg ccattgccgt gaccgaccat gactcagtag ccggcatcga tgaggcgacg
     3721 tcgaaaggcg ccgaagtcgg cgtggagatt gtgcccggct tggagctttc cacggatgtc
     3781 gacgacggag aggtccatat actggggtac ttcatagatc gagaggataa agaccttctg
     3841 gatctcttgg aatcgcagcg cgaaagcagg ctgcaacgag tgcggaagat gctggatcgg
     3901 ctatccggaa tgggtgtgca gctgagcatg gacgaggtgc aaagattctc agacggcggt
     3961 cctctcgggc gaccgcacgt ggcccaggcg ctgatacggg cgggtcaggt gggttcatgg
     4021 gatgaggctt tttcacggta tatcggcagg catgcgccgg catacgtcag gcggtccaaa
     4081 ctctcgcctc acgatgcagt ccgagccata ttggcctccg gcggtgttcc ggtgctagct
     4141 catcctggtc tatccggaca tgatgacatg atcccttcgc tggtggac
//
LOCUS       DAOTAC010000233         4133 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_17530_length_4133_cov_5.677048, whole genome shotgun sequence.
ACCESSION   DAOTAC010000233 DAOTAC010000000
VERSION     DAOTAC010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4133
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17530_length_4133_cov_5.677048"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..87)
                     /locus_tag="PLM74_09400"
     CDS             complement(<1..87)
                     /locus_tag="PLM74_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_045009167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HON43325.1"
                     /translation="MERVAYGRGYAYSVQYHIVWCVKYRRKII"
     gene            341..1714
                     /locus_tag="PLM74_09405"
     CDS             341..1714
                     /locus_tag="PLM74_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017714397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HON43326.1"
                     /translation="MRNLIEDALRSDGADYVDIRIEDAVRTKITLRGRSTDELSVSRS
                     TGGCVRALAGGGWGFISFNDIANLKHHVALAVSQARLVSQANGDSLLAPVGPVVDTVV
                     PHLVVDPRSISLAAKQEMLQEYSQIMLGASPKIQTTTVRYRDTYSKKIFASSEGAFIE
                     QEHCDVGASCRAIAREGDNVQQMFIVAGGVDGYQSVEGQHDRARECARRAALNLDAAP
                     VKAGQYTVVLDPAMAGTFVHEAFGHLSESDFIYENDTLRDMMVLGRQFGSPILGITDG
                     AAAAGHRGSYRYDDEGTPASLTPLIREGVLVGRLHSRETAAKMGERPTGNARAISYRF
                     RPIVRMTNTYIENGEMSFEEMISDIDEGIYAIDAYGGTTAMEMFTFSAMEAFMIRKGR
                     IAERVRNVVLTGNVFETLMHIDAVGSDLRMNDGGTGGCGKGEQYPLPVSDGSPHIRIR
                     NVVVGGN"
     gene            1717..3054
                     /locus_tag="PLM74_09410"
     CDS             1717..3054
                     /locus_tag="PLM74_09410"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HON43327.1"
                     /translation="MIDLLDMAVRRADAAEFYLVSSEAIPATFEMNKLKHVETRRMQG
                     FALRVIKSGRLGFSSSTDTSGVAAQELINAAVDSADMGEPARFDLPGPVLGGKSAAPL
                     IYDDKIAELDADALIRMGREIVGIITEYDPSIKCGVQLERKVEEVTVANSNRGGGNYR
                     KTTLTAMADALLVDGNDIFEVIGMDGSCRSDLDHRSVAHEMLERVRLGRRIAEVHTGR
                     MQVLFTHRGIMPLLMALQSALNGKTALEGASPLAGRIGQQALDPRVCIYDDPTVDWAL
                     GSCPIDGEGVQSRRTPLVEAGIVKGFYYDLQTAGRAGTSSTGNGFRRAGAGDYFEGQP
                     TPTLTNAMVAPGSESLDDMITSMDEGIIVDQVMGAGQGNILTGDFSVNVHLGFKVEKG
                     KIVGRVKDTMVAGNAMEALNNIAAISDCAEWAFGEYSSPAFLFSSLGVAAKGR"
     gene            complement(3124..3441)
                     /locus_tag="PLM74_09415"
     CDS             complement(3124..3441)
                     /locus_tag="PLM74_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005814555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HON43328.1"
                     /translation="MEKTPQHRVIVSDRARQMLASHVRFLAQKSPSAAREAKDQLADA
                     IRSLHTMPERFPFLDAQFVPVNKYHKMCVGRWYLILYQIKDQTVYVDYVVDCSQDYGW
                     LVR"
     gene            complement(3419..3709)
                     /locus_tag="PLM74_09420"
     CDS             complement(3419..3709)
                     /locus_tag="PLM74_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007130980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HON43329.1"
                     /translation="MQIKPSARIRQNYNEIAALCKSSGEPVYLTKNGEGDLVVMDIDA
                     FERREKMLRLREELLAVEEDRLTGRAGTSPEELDAYLESIIAEVEHGKDASA"
     gene            complement(3882..>4133)
                     /locus_tag="PLM74_09425"
     CDS             complement(3882..>4133)
                     /locus_tag="PLM74_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43330.1"
                     /translation="PARAVIIAGRDSVLKRLLVMPLHHLHAEEVRRFCISGLPFSICT
                     SVSFRPSPTAVSRQERLLTMHIRKWSRRLRKWGRRERRA"
BASE COUNT          939 a         1192 c         1210 g          792 t
ORIGIN      
        1 tattatcttc ctgcgatact tcacgcacca tacaatatga tactgaactg agtaggcata
       61 ccctcgccca tatgctactc gttccaattc atccacctct atgcagcata acacatgttt
      121 ggataggtac acgcaattgc aggctattct gacagttctt gggtaacata tgtcgacgct
      181 tactcacgac tgaagtcacg agcatgcgcg gcggttcaat caagatcatt cgatcatcgg
      241 ggatagactc ctccaaagca cgaaagatcc ctgcagaact gacaggttga aggatactgc
      301 ggtccagtgt ccaagttctg cgcaaagggg gcatgggatt gtgcgaaacc tgatcgaaga
      361 tgcgctgaga tcggatggcg cggactatgt agacatcaga atcgaagatg ccgtgcgaac
      421 caagataacg ctgcgcggca ggagcacgga tgaactgtca gtctcccgtt cgacgggagg
      481 atgcgttcgc gctctggccg gaggcggctg gggattcatc tcattcaacg atatcgcgaa
      541 cctcaaacac cacgtagcgc tggccgtaag ccaggcccgg ctggtatcgc aggcgaatgg
      601 agacagcctc ctcgcccctg tcggaccggt ggtcgatact gtagtgccgc atctagtggt
      661 tgacccgaga tcgatcagcc ttgcggccaa gcaggaaatg cttcaggaat acagccagat
      721 catgctgggc gcgagcccca agatccagac gaccacagtc aggtacagag atacttattc
      781 gaagaagatt ttcgcaagct ccgaaggcgc cttcatcgag caggagcatt gcgatgtcgg
      841 cgccagctgc agggctatcg cgcgagaggg cgacaacgtg cagcagatgt tcatcgttgc
      901 cggaggcgtc gacggctacc agagcgtcga ggggcagcac gatagagccc gcgagtgcgc
      961 caggcgcgca gccctgaatc tcgatgcggc gccggtcaaa gccggccaat acactgtagt
     1021 gctcgatccg gccatggcag gtacattcgt gcacgaagcc ttcggccacc tgagcgaatc
     1081 cgacttcatc tacgaaaacg ataccctccg cgacatgatg gttctgggca gacagttcgg
     1141 ttccccgatc ctgggcatca cagacggcgc cgccgcggcg ggccacagag ggtcgtacag
     1201 gtacgacgac gagggcacgc ccgcctcgtt gacgcctctc ataagggagg gcgtgctcgt
     1261 gggcaggcta cattcgcgcg agactgcggc caagatgggc gaacgtccca cgggaaatgc
     1321 ccgcgcgatc agctaccggt tccgccccat tgtgcgaatg accaacacct acatcgagaa
     1381 cggcgaaatg agtttcgagg agatgatctc cgacatcgac gagggcatct acgccattga
     1441 cgcctatggc ggcacaacgg ccatggagat gttcacattc agcgcaatgg aagccttcat
     1501 gatccgaaag ggcagaatcg ctgaaagagt gcgtaacgtg gtcctcaccg gcaacgtatt
     1561 cgaaacactg atgcacatcg acgccgtcgg cagcgatctt cgcatgaacg atggcggtac
     1621 cggagggtgc ggcaagggcg agcagtatcc gctgcctgta tccgacggca gcccgcacat
     1681 acggatccga aacgttgtgg tgggagggaa ctagcgatga tagacttgtt ggatatggcg
     1741 gttaggcgcg ccgacgcggc tgagttttac ctcgtaagca gcgaggccat ccccgcaacg
     1801 ttcgagatga acaagctcaa gcatgtagag acccgcagga tgcagggatt cgctctgcgc
     1861 gttatcaagt cgggcagact agggttctcc tcctccaccg ataccagtgg agtcgcagcc
     1921 caagaactca tcaacgcggc ggtcgactcc gccgacatgg gagaacccgc gagattcgac
     1981 cttcctgggc cggtcctggg cggcaagtcc gctgcgccgc tcatctacga tgacaaaatc
     2041 gcagagctcg acgcggacgc tctgatccgc atgggtcggg agattgtggg catcatcaca
     2101 gaatacgacc cctccatcaa gtgtggtgtg cagcttgaga gaaaggtcga ggaagtcacc
     2161 gtagccaact cgaaccgcgg aggcggcaac tatcgcaaga caaccttgac tgcaatggcc
     2221 gatgccctgc tggtagatgg caatgacatc ttcgaagtga tcggcatgga cggttcatgc
     2281 cgcagtgacc tcgaccatcg atccgtcgcg catgagatgc tggaacgcgt gagactcggg
     2341 cgcaggatcg ccgaggtgca cacgggccgt atgcaggtgc ttttcaccca ccgcgggatc
     2401 atgcccctcc tcatggccct gcagtcggcg ctaaatggca agaccgcgct ggaaggcgca
     2461 tcgcccttag caggcaggat aggccaacag gcactggatc cacgagtctg catatacgat
     2521 gatccaactg tggactgggc actcggcagt tgcccaatag acggcgaagg agtgcaatcg
     2581 cgcagaacgc cccttgtaga ggcaggaatc gtgaaaggtt tctactacga tcttcagacc
     2641 gcgggcagag cgggcacatc cagcacaggc aatggcttca ggcgggccgg cgccggcgat
     2701 tacttcgagg ggcagccaac ccccaccctg accaatgcca tggtagcgcc gggcagcgag
     2761 agccttgacg acatgatcac atccatggat gaaggcatca ttgttgacca agtgatgggc
     2821 gccgggcagg gcaacatact gaccggggac ttctccgtca acgtgcatct cggtttcaaa
     2881 gtagagaaag gcaagatcgt aggccgcgtg aaggacacaa tggtcgctgg caacgccatg
     2941 gaggcgttga acaacatcgc cgccatcagc gattgcgctg agtgggcctt cggggagtac
     3001 tcctcccccg cgttcctctt cagctccctg ggagtagcag cgaaaggaag ataagcagtc
     3061 tccgttcggc gtgggcgcac ggcaccgacc gagaacggat ctgcccacgt cgaacaccgt
     3121 gactcacctc accagccatc cgtagtcctg actgcaatcc acgacatagt cgacgtacac
     3181 tgtctggtcc ttgatctggt acaggatgag ataccacctg ccaacacaca tcttgtggta
     3241 cttgttgact ggtacgaact gggcatcgag aaacggaaaa cgctccggca tcgtatgaag
     3301 agagcggata gcgtcggcca gctggtcttt cgcctctcga gcggcagatg ggctcttctg
     3361 cgccagaaac cgaacgtggc tagccagcat ctgacgggcg cggtcggaga caatcaccct
     3421 atgctgaggc gtcttttcca tgttccacct cggcaatgat gctctctagg taggcgtcca
     3481 gttcctctgg agaggtcccg gcgcgaccag tcagacggtc ttcctccacg gccagcagtt
     3541 cctcgcgtag tctcagcatt ttctcgcggc gttcaaacgc atctatgtcc atgaccacaa
     3601 gatcgccctc gccgttcttc gtgaggtaca cgggctcacc tgaggacttg cacaaggccg
     3661 cgatctcatt gtagttctgc ctgatgcgcg cggatggctt gatctgcaca ggatcaactc
     3721 cccgtagcaa cattctgatc atattctacc tctttgatgc tatgacatca aggtactgat
     3781 gcttgtgtac tgatgcttgt gctccaaaca tcgcttttcg caccgattcc gatgtccctc
     3841 cggctgcagc aggccagctg aagagaggtt tgccgcgctc atcatgcccg gcgctcccgt
     3901 ctgccccatt tccggagcct tcggctccac ttccgaatgt gcatggtgag aagcctctcc
     3961 tggcgcgaaa ccgctgttgg ggaaggccga aaggaaacgg aagtgcagat ggagaaaggc
     4021 agccctgata tgcagaaccg ccggacttcc tcggcgtgaa gatggtgaag gggcatgacc
     4081 agtaaccgtt tcagaaccga gtctctgcct gcgataatga cagctctcgc agg
//
LOCUS       DAOTAC010000234         4126 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_17569_length_4126_cov_5.943503, whole genome shotgun sequence.
ACCESSION   DAOTAC010000234 DAOTAC010000000
VERSION     DAOTAC010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4126
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17569_length_4126_cov_5.943503"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            397..531
                     /gene="rpmH"
                     /locus_tag="PLM74_09430"
     CDS             397..531
                     /gene="rpmH"
                     /locus_tag="PLM74_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HON43331.1"
                     /translation="MKRTFQPKVRRRAKIHGFLARMSTPGGRRVIRRRRARGRKRVSA
                     "
     gene            615..1019
                     /gene="rnpA"
                     /locus_tag="PLM74_09435"
     CDS             615..1019
                     /gene="rnpA"
                     /locus_tag="PLM74_09435"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HON43332.1"
                     /translation="MRAHSLRRTSEFNRAFREGKAAAGKYVVVHAVPNGLEIVRVGFP
                     VGKKMGGAVKRNRVKRLLREAVRLSGAMPNQGYDLVVVPRRRMTSPDLRCADVVADLG
                     RALEQLGVCSTREMTGDPGASPREEASDAREA"
     gene            1003..1218
                     /gene="yidD"
                     /locus_tag="PLM74_09440"
     CDS             1003..1218
                     /gene="yidD"
                     /locus_tag="PLM74_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007070240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HON43333.1"
                     /translation="MLEKLELQVIRLYRVYVSPLKRGPCCRFVPTCSQYAEEAVRKHG
                     ALRGSCLALWRLLRCHPFSRGGYDPVP"
     gene            1276..1908
                     /locus_tag="PLM74_09445"
     CDS             1276..1908
                     /locus_tag="PLM74_09445"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HON43334.1"
                     /translation="MSITSVMESILRYFTHLTGNLGWAIIIITVLIKAILHPLQVNQI
                     KMMEGMKALTPKIKELEQKYKGQPQEYQQRLAELYRDNKVNPMAGCLPALIPLPVLVV
                     FFRVLQNKAFVDSFGAAKTSFLWLPSLSAPDPWILPILSAATMWFSMTQSTTDQSQKT
                     MTIVMPLFFGWFTRTMPSGAAVYWVATNVVSILQHYLIQRQLAAPKKGVS"
     gene            1909..2733
                     /gene="jag"
                     /locus_tag="PLM74_09450"
     CDS             1909..2733
                     /gene="jag"
                     /locus_tag="PLM74_09450"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HON43335.1"
                     /translation="MEPIEVAGKTVDEAVQTGLSSLGLRKDQVEIEVIDEGSRGFLGL
                     VGFRHARVRIRALNAGSTSVGDTASGRTCPRPRLQTAQNASALPEDGSSGDVAGQRGI
                     CVRGVDADHGYIDRRAVDEGDLSCGAKFLEGLVSVMGLDAEVREFPEEDITMLEVEGK
                     SLGVLIGRHGETLDSIQYLVNLAANRAIRASGEEERVRYVVDVAGYRRKREETLRAMA
                     VEATDKVIEDRKKQILEPMSALERRIVHMALKDVNGVVTHSEGREPYRRIVISPTE"
     gene            2882..>4126
                     /gene="mnmE"
                     /locus_tag="PLM74_09455"
     CDS             2882..>4126
                     /gene="mnmE"
                     /locus_tag="PLM74_09455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HON43336.1"
                     /translation="MSDWTAYAADTIAAVATPLGMGGIAIVRVSGSSAQQVVDRVFRP
                     VRRHRSGESLPEHRRGWHATYGRVYDSSGEFIDEAIMLSMPGPASYTREDVVELSCHG
                     GPAVVRAVLQAVLDAGARHAGPGEFTKRAFLNGRIGLGQAEAILQVINARTERARRSA
                     SAFLSGKSGRMAQESREAVAQLITIIEASLDFPEDDIPEMQLLEIGNEARRIAMDLCR
                     AASDAHRGRVLWEGASVAIVGRPNVGKSRLLNALLREERAIVSERPGTTRDTVSEVAN
                     IRGIPIRLIDTAGMREAADPLERIGVERARGALADADIALAVIDGSIRLVQEDFDIVG
                     STYEDRTVVVVNKSDRPAAFADEDLGQHVGNRRILRVSALTRSGIDQLEAEIEALAMA
                     RHADVGEGIQSFGARRADCLRRA"
BASE COUNT          893 a         1071 c         1304 g          858 t
ORIGIN      
        1 tcttcgcaca cgttatccac agattgatcc acagtctgtg cataaacagg cccacaactg
       61 gcgataatca tgattgcatg gaataggcga tgaaaaattc atccgtttgc aaagactatc
      121 ataacagact tgcgggtacc aggacagtct gaatggaagc gtttgaaagg tccctgtgga
      181 taaccgtacg tagatgcgag tcgcatcgaa accgcaccaa aaccgcaccg aaagcgcgaa
      241 tggattgctg cctagacccg cccgcctgga ctgcctggat tgctgcctag atcggcccgc
      301 ctggaccgcc tggatcgctg cttgacactg catgtttacg tcgcttataa ttgacgcggc
      361 atgcttgcgg tggagtttac agggaggaac gcttggatga aacggacatt tcaacccaag
      421 gtacgtcgca gggcaaagat tcacggtttt ctggctcgca tgagcacgcc cggcggtcgg
      481 agagtgataa ggcgcagacg cgccaggggc cgcaagcggg tttcggctta agctgacctg
      541 ttgcgtctcg aagttctgcc gtcgtgcgac ggcttttcct ttcgtatcgg cgtttgtggc
      601 aggggggccc cagcatgcgc gctcacagcc tgcgtaggac ctcggagttt aacagggctt
      661 tccgcgaagg caaggctgct gcaggcaagt acgtcgtggt ccatgctgtg cccaacggcc
      721 tcgaaatcgt gcgcgttggg ttccctgtgg ggaagaagat ggggggcgct gtgaagcgca
      781 acagagtcaa acggctgttg cgggaagcag tgcgtctttc aggggccatg ccaaaccagg
      841 gctatgacct cgttgtcgta ccgaggcggc gcatgactag cccagatctt cgttgcgctg
      901 acgtcgtggc agatctgggc agagcacttg agcaattggg cgtatgctcg actcgggaga
      961 tgacggggga tcctggcgcg tcgccgcgtg aggaggccag tgatgctcga gaagcttgaa
     1021 ctgcaggtaa ttcggctata ccgggtctac gtctctcctt tgaagagagg tccgtgttgt
     1081 cgttttgtac ccacgtgttc gcaatacgcc gaggaggcag ttaggaaaca cggggcactc
     1141 cggggttcgt gcctagccct ctggcgactt ctcagatgcc acccgttcag tcggggagga
     1201 tacgatcccg ttccatagag ccgactacta ccatattacc agctacagtc ctctgactga
     1261 aagggggagg ctgcattgag catcacctcg gtgatggaga gcattctgcg gtactttacg
     1321 caccttaccg gcaatctcgg gtgggcgatc ataataatca cagtgctgat caaggcgatt
     1381 ctccatccgc tgcaggtaaa tcagattaag atgatggaag gcatgaaggc gcttaccccg
     1441 aaaatcaagg aactggaaca gaagtacaag gggcaaccgc aggaatacca acagcgcctt
     1501 gcagaactgt accgcgataa caaggtcaac cctatggccg ggtgccttcc ggctctgatc
     1561 ccactacccg tgctcgtcgt gttcttccga gtactgcaga acaaggcttt tgtcgatagt
     1621 ttcggcgcgg ccaagactag ttttctctgg ttgccaagtc tcagcgctcc cgacccgtgg
     1681 attctgccca tcctgtcggc ggccacgatg tggttttcca tgactcagtc tacaacagat
     1741 caatcacaga agacgatgac catcgtgatg ccgctgttct tcggttggtt caccagaacc
     1801 atgccctcag gggcggccgt ttactgggtc gctacgaacg ttgtcagcat actccaacat
     1861 tacttgatcc agaggcaact ggctgcgcct aagaagggag tcagctagat ggaaccaata
     1921 gaggttgctg gcaagacggt ggacgaggca gttcaaaccg gattgtcgtc cctcgggttg
     1981 aggaaggatc aggtcgagat agaggtcata gatgagggtt ctcgcggatt cctgggcctg
     2041 gttggtttcc ggcacgcgag agtccgcatt cgagcgttga acgcgggttc tacctcggtc
     2101 ggcgatacgg cctctggccg cacatgcccg agaccacgtc tgcagacggc gcagaacgca
     2161 tccgcattgc cggaggatgg ctcgagtgga gacgttgccg ggcagcgtgg catttgcgta
     2221 cgcggagttg acgccgacca tggctacatc gatagacgcg ctgtggatga gggcgacttg
     2281 agctgcggcg cgaagttcct cgagggactt gtgtcggtca tgggcctaga cgccgaggtg
     2341 cgcgagtttc ccgaggaaga catcacaatg cttgaggtcg agggcaagtc gctcggagtg
     2401 ctcatcggtc gacacgggga gacgcttgat tcaatacagt atctggtcaa cctcgcagcc
     2461 aacagggcca ttcgggcctc tggcgaggag gagcgggtca ggtacgttgt ggatgttgca
     2521 ggctacagga ggaagcgaga agagaccctc agagccatgg cagtggaagc cacagataag
     2581 gtaattgaag acaggaaaaa gcaaatactg gaacccatgt cggctctgga gcggcgcatt
     2641 gtccacatgg ctctcaagga tgttaacggc gtcgtaactc attcagaggg tcgtgagccc
     2701 tacaggcgga tagtgatatc gcccaccgaa tagcagaaga aatgctgaaa atgcgatatt
     2761 gtgaaagtgc gtgttcaaaa agcgcgcact ttcttttatg ctgatatatg aagcggattg
     2821 tttcacgtga aacaattgtc gggattagcg atcccgagat cgacgcgcgg gaggtgcgga
     2881 tgtgtcggac tggacggcat acgctgctga cactattgca gccgtggcta cacctctggg
     2941 gatgggcggc atagccattg tcagggtgtc gggatcaagt gcccagcagg tggtggacag
     3001 agtgttcaga cccgtccgtc ggcatcggag cggcgaatcg ttgcctgaac accgccgcgg
     3061 atggcacgcg acctatggtc gagtttatga ctcttccgga gagttcatcg acgaggctat
     3121 tatgctgtcg atgcctggcc cggcttcgta cactagggaa gacgttgttg agctgagttg
     3181 ccacggtgga cctgcggtcg tacgggcagt gttgcaggcg gtactggacg ccggagcgcg
     3241 ccacgcgggg cccggggagt tcacaaaacg ggcctttctt aatggacgca taggcctggg
     3301 acaagctgaa gccatactgc aggtcataaa tgcgcgcact gagcgggccc ggcgctccgc
     3361 ctcggccttc ctatccggga aatctggccg catggcacaa gagtcgcgag aggccgtggc
     3421 tcagctcatt accatcatcg aggcttcctt ggattttccc gaggacgaca ttcccgagat
     3481 gcaattgcta gagatcggca atgaggcccg gcgcattgcc atggacctat gtcgtgctgc
     3541 ttctgacgcg cacagaggcc gggttctctg ggaaggcgcc agtgttgcaa ttgtcggaag
     3601 gcctaacgtg gggaaatcac gtctgctcaa cgctctgctg cgcgaagaga gggcgatcgt
     3661 atcggaacgg cccggcacta ccagggacac ggtgtcggag gtagccaaca tccgcggtat
     3721 tcccatacgc ctgatagaca ctgcgggaat gcgcgaggcg gccgatcctt tggagcggat
     3781 cggtgtcgaa cgcgccaggg gtgcactggc cgatgctgat atcgccttgg ctgtgatcga
     3841 tgggtcaata cggctcgttc aggaggactt cgacatcgtg gggtccacat acgaagaccg
     3901 cacggtagtc gttgtcaaca agtcggatcg tcccgcagcg ttcgctgatg aggatcttgg
     3961 gcagcacgtg ggcaaccgac gcatcttgcg agtctcagca cttacaagga gcggaataga
     4021 tcagttagaa gcggaaattg aggcgctggc catggcgcgg catgccgatg tcggggaggg
     4081 aatccagtca ttcggggcgc gcagggctga ctgccttcgt agggcg
//
LOCUS       DAOTAC010000235         4115 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_17627_length_4115_cov_4.062069, whole genome shotgun sequence.
ACCESSION   DAOTAC010000235 DAOTAC010000000
VERSION     DAOTAC010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4115
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17627_length_4115_cov_4.062069"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1461)
                     /gene="alaS"
                     /locus_tag="PLM74_09460"
     CDS             complement(<1..1461)
                     /gene="alaS"
                     /locus_tag="PLM74_09460"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HON43337.1"
                     /translation="MKTDELRQAYLKFFESRGHRILPSASLIPAGDPTLLLTAAGMVP
                     FKPYFLGTAKPDHSRATTCQKCVRTGDIENVGRTTRHATFFEMLGNFSFGDYFKTEAI
                     TWGWEFVTRVLGFSPEQLWITIYLDDDEAFEIWNKTVGVPADRIVRKGKKDNFWEIGV
                     GPCGPCSEIHVDRGADVGCGSADCGLDCDCGRFIEIWNLVFIQFHRDEQGNYTPLEHK
                     GIDTGMGLERVAAVLQNVPTIFDTDETRIIRDLAAGLAGVTYGEGGLSDMAVRVITDH
                     VRASVFMVGDGILPTNEGRGYVLRRLLRRAARFGKLIGIEGVFLPKVAGTAIDVMGNT
                     YPELADDRERILRSIQLEEERFLVALDQGSAVLESVIARVQSEGGSCVPGADAFRLYD
                     TFGFPFELTVEIAGERGLTVDQSGFDRAMEAQRERARAARGENAYLDETSERYHAAIG
                     EATTEFVGYDRLAEQARVIAVIGADGTATTRLDLIDA"
     gene            complement(1631..2041)
                     /locus_tag="PLM74_09465"
     CDS             complement(1631..2041)
                     /locus_tag="PLM74_09465"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HON43338.1"
                     /translation="MRAEIVNVDGLTMMAKSDTNHWVVMDTTREGQVAAAATPMETFL
                     FGFGGCFCMTVKSLLLKKRLDVSKVSMRIDAQRAPEPPTVFTKIDAVLELSGSNLRDA
                     DVEWAVALAREKYCQVDVMLEKAVPIDVKWEILE"
     gene            complement(2184..3212)
                     /locus_tag="PLM74_09470"
     CDS             complement(2184..3212)
                     /locus_tag="PLM74_09470"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HON43339.1"
                     /translation="MAPSKHAGILRSLGTALAVLALLLFAYRVRRAFIPFFFAIGIGY
                     IISPLVRMAEQRGAPRAAAIFTAYVLVAAVIGFSLAFVLPPLATQLDEIVGAFSPDAS
                     ADGLAADWRRRLQLLPEGAASRLATAAVDRAEVYISEVARAAGRAVSALTSQLVNIVL
                     APVIAFYLTRDVDAIRERFASWLPAVSRNRIMSVISDIDRALAGWVRGQVIVCTFVGL
                     ATGTSLALLGVRYALPIGFLTGILEIIPYFGPVLALVPAVLLAAQKSSVTALVAATVF
                     VAIQQIESAVVSPKIVGDCVGLHPLVVIGALLVGGALFGVVGMLLAVPVTAVLFILGQ
                     RLLVDPAR"
     gene            complement(3285..3464)
                     /locus_tag="PLM74_09475"
     CDS             complement(3285..3464)
                     /locus_tag="PLM74_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43340.1"
                     /translation="MNSSRSGKKIVHEELSTETPMGTQRTVSRTVSRRRSYAEPVVEA
                     MQSMGRRFRSGDDSD"
     gene            complement(3594..4034)
                     /gene="nifU"
                     /locus_tag="PLM74_09480"
     CDS             complement(3594..4034)
                     /gene="nifU"
                     /locus_tag="PLM74_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002597364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HON43341.1"
                     /translation="MYNDKVMDHFTNPRNVGDIPDADGVGQVGNPVCGDIMRIAIKVE
                     DGVIQDIKFKTFGCGAAIATSSMVTELVKGRSLKEALDISNKAVAVALGGLPPVKMHC
                     SNLAADALHLAIKDYIEKHGGDGLGIDPASIVVEEHDHGEEADA"
BASE COUNT          872 a         1369 c         1090 g          784 t
ORIGIN      
        1 cgcgtcgatg aggtcaaggc gagtggtggc cgtgccgtcc gcgccgatca ctgcgatgac
       61 gcgagcctgc tccgccagcc tatcgtaacc cacaaactcg gtagtcgctt cccctatggc
      121 cgcgtggtac ctctccgacg tttcgtccag atacgcattc tcgccccgag ctgcccgcgc
      181 ccgctcgcgc tgggcctcca tggctctgtc gaaaccgctc tgatccaccg tgaggccgcg
      241 ttcgccggcg atctccactg tcagttcaaa tggaaaaccg aatgtatcgt acaaacggaa
      301 cgcatccgcc ccaggcacac acgacccgcc ctccgactgc acgcgggcga tcaccgattc
      361 cagcactgcc gatccctgat ccaaggcgac caggaaccgc tcttcctcca gctggatgct
      421 gcgcagaatg cgctcgcggt catcggcaag ctccgggtag gtgttgccca tgacgtctat
      481 ggcggtgccc gctaccttcg gcaggaacac accttcgata ccgatcagct tgccaaaacg
      541 cgcagctcgg cgcaggagcc gacggagcac atatccacgg ccctcgttgg tgggcaggat
      601 gccgtcgccc accatgaaaa cagacgccct gacatggtca gtgataaccc gaaccgccat
      661 gtcggacaga ccgccctcac cataggttac gccggccagc ccggctgcca agtcgcgtat
      721 gatccgagtt tcatcggtat cgaatatcgt gggcacattc tgcagcacgg ctgcgacgcg
      781 ctccagcccc atcccggtgt caatgccctt gtgctcaagc ggagtgtagt taccctgctc
      841 gtcgcggtgg aactgtatga agactagatt ccagatctcg atgaacctgc cgcaatcgca
      901 gtccaggccg caatcggcgg acccgcagcc tacgtcggcg ccgcggtcga catgtatctc
      961 cgaacacgga ccgcacggcc cgaccccgat ctcccagaag ttatctttct tgcccttgcg
     1021 gactatcctg tctgcgggta cgcccacagt cttgttccat atctcaaatg cctcatcgtc
     1081 atcaaggtag atggtgatcc aaagctgctc ggggctgaac ccaagcactc gagtgacgaa
     1141 ttcccagccc caggtgatgg cctccgtctt gaagtagtca ccaaatgaga agttcccgag
     1201 catctcaaag aatgttgcgt gacgcgtggt ccgcccgacg ttttcaatgt cgccggtacg
     1261 cacgcacttt tgacacgtgg tggcgcggga gtgatctggt ttcgccgtgc ccaggaagta
     1321 cggcttgaac ggaaccattc cggcagccgt aagcagcaat gtaggatcgc ctgccgggat
     1381 caacgatgcc gacggcaaaa tcctgtgccc ccgggactca aagaacttca gataggcctg
     1441 cctcaattcg tcagtcttca taagcaacac ctcacttgca cggccaaagc agcatcgaat
     1501 gcgccgcggc gaccgcccgt tcggactgtc gccgattctc aaatcacctt atcataacaa
     1561 cggttcacac acaatggtct acctgcagag gtcacatatg tcaggctcct aaggccgcag
     1621 cgccgtgcgt ctactcaaga atctcccact ttacgtcgat aggtactgcc ttctccaaca
     1681 tcacgtccac ctggcagtac ttctcgcgcg ccagcgcaac cgcccattct acgtcggcat
     1741 cgcgcaagtt gctgcctgaa agctccaaca cggcgtcgat cttcgtgaac acagtgggcg
     1801 gctcaggcgc ccgctgcgcg tctatcctca tcgatacctt acttacgtca agcctcttct
     1861 tgagcaggag cgacttcaca gtcatgcaga aacacccgcc gaacccgaac agaaacgtct
     1921 ccatgggagt agcagcagcg gctacctgcc cctccctcgt ggtgtccatg accacccagt
     1981 gattagtgtc ggacttcgcc atcatggtca atccgtccac attgactatc tcagcacgca
     2041 tgagtcctcc tccttatacc atcccgattc gtctacttcg tgaggtggta gagtgattta
     2101 ctttctacaa ggtcgaccct tctcctccgt gctccgcccc agcaccctcc aggggcccag
     2161 gcgcacgtga ccgaaaaggc cccctaccgg gccgggtcca ccagcaggcg ttggcccagg
     2221 atgaacaaca cagccgtaac aggcactgcc aataacatgc cgaccacacc gaacagcgcg
     2281 ccaccaacaa gcagcgcccc gatgaccacc agagggtgca ggcctacgca gtctccaacg
     2341 attttgggcg agaccactgc cgactcaatc tgctgaattg caacgaacac cgttgcggca
     2401 acgagtgcag tcactgagga cttctgagcg gccagaagga cggctggaac cagcgcaagt
     2461 accgggccga agtaggggat tatctcgagt atgccggtca gaaacccaat cgggagggcg
     2521 taccgcacgc ccaaaagcgc gagggaggtc ccggtcgcca accccacgaa cgtgcatacg
     2581 atgacttgtc ctctgaccca gccggccagc gccctgtcga tatccgatat aacactcata
     2641 atcctgttgc gcgaaaccgc aggaagccac gaggcgaacc tctcgcgaat ggcgtccaca
     2701 tcgcgcgtca ggtagaacgc gattaccggt gccagcacta tgttgaccag ctgcgatgtg
     2761 agcgccgaca cagcgcgccc tgctgcccgc gccacctcgc tgatgtagac ctcagcacgg
     2821 tccacggccg cggtcgccag tcgcgatgca gccccctcag gaaggagttg cagtcggcgc
     2881 cgccaatcgg cagccagccc gtcggcgctt gcgtcgggcg agaatgctcc cacgatttca
     2941 tcgagttgtg tagccagggg cggcagcacg aacgcaagcg aaaagccgat cacagcagcc
     3001 accagcacgt acgcagtaaa gatagcggca gcccggggcg ctccacgctg ctcggccatt
     3061 ctcaccaacg gacttataat gtagccgatc cctatggcaa agaaaaatgg tatgaatgcg
     3121 cgcctgaccc ggtaggcgaa aagaagcagc gcgagcacgg cgagtgccgt gccgagcgaa
     3181 cgcaagatcc cggcatgttt cgacggcgcc atgctcgcac ctcagcgcaa atggaccggc
     3241 ccgagcgcgg cgcgtccgga accggtccgt atcttggcgg cagttcagtc cgagtcgtca
     3301 ccggacctga agcgccgccc catcgattgc atcgcttcga ctacgggctc agcgtacgac
     3361 ctccgtctgg agacagtccg gctgaccgtg cgctgggtgc ccatgggagt ctcagtcgat
     3421 agctcctcgt ggacgatctt cttgccggaa cgggacgagt tcatcaacat gagcacgcct
     3481 agacctgcgc caatgagact gccggtaatg agcgccctcc acaaccttct gatggtcgtt
     3541 ccctcctctc gcaggacgta ttatgcccct tgcgcaagga gagtaaacca ttgttaggcg
     3601 tctgcctcct cgccgtggtc gtgctcttct acgacaatcg aagcagggtc aatgcccaat
     3661 ccgtcgccgc catgcttctc aatgtagtct ttaatcgcca ggtgcagcgc atcggcagcc
     3721 aggttggagc agtgcatctt gaccggaggc agcccaccga gggccactgc gacagccttg
     3781 ttcgagatat cgagcgcctc cttgaggctc ctacccttaa ccagctccgt caccatactc
     3841 gaagtggcga tcgccgctcc gcacccgaag gtcttaaact tgatatcctg gatgactccg
     3901 tcctcgactt taatcgctat tctcattatg tcgccgcata cagggttccc aacctgaccc
     3961 acaccgtcag cgtccggaat gtcgccaaca ttgcgtggat tagtgaagtg atccattacc
     4021 ttatcgttgt acaattctca cacctcctgc cgactgcggc acgaagagga tcatgttgcc
     4081 gcagcccaag acgcatcctt tggttctcct gctct
//
LOCUS       DAOTAC010000236         4046 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_17975_length_4046_cov_3.328489, whole genome shotgun sequence.
ACCESSION   DAOTAC010000236 DAOTAC010000000
VERSION     DAOTAC010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17975_length_4046_cov_3.328489"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1479
                     /locus_tag="PLM74_09485"
     CDS             <1..1479
                     /locus_tag="PLM74_09485"
                     /inference="COORDINATES: protein motif:HMM:NF028259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43342.1"
                     /translation="GFYFSRDSSILTPLPLPASTVVMARFGVVLINEYITLALVLVPA
                     FCVYGIRSGAGLLYWITCIPVFLLTPVIPLAIDAAVAILLMRFVNVRKNRTLFMFLGT
                     AIFLGLYFWFQYYIMHQAPQSEADMLNYLMTVQDSLSRSMAMRFPPSLWASFSLSKAG
                     TAAGLGNLAILVAVSAGALLLALALGSRLFYAALAAGGEVAVSAAGRKVGRWGRRQPT
                     QTAGDLALPAAWWVQRSPEVAIALKDMWLFVRTPTYLLNGLANVLVFPGLLAVWFIAG
                     GRGGVGGGAGVGNPFAEIPGFAAFMSSPEFAAARALAFAGAILFVSGINAVASSAFSR
                     DGMQYWVYKVIPVSFRRQVIGKIMFALAFQFASMLPLVVVMQLILRLDLAALAAGVLI
                     GVAGSTWASLTSLYVDMMRPYLTWDNPQRAMKSNLNALIAMAAVTVVAVGAGYAIVKA
                     LSAGLDQHAVIWCTFVFFAALSILSYRLLMSGAGRAFRRVEL"
     gene            1811..2926
                     /gene="thrC"
                     /locus_tag="PLM74_09490"
     CDS             1811..2926
                     /gene="thrC"
                     /locus_tag="PLM74_09490"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017247553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HON43343.1"
                     /translation="MRLWRGIIEEYREFIPFGAGAPIVTLLEGGTPLVPAPALARRLG
                     VRADIRLKLEGANPTGSFKDRGMTAAITKAVADGARVVICASTGNTSASAAAYAARAG
                     LRCVVVIPDGAIAMGKLAQAIAHGAEVVPIRGNFDQALAIVREMADAGIATLVNSVNP
                     WRIEGQKTAAFEIVDQLTSGGHGAPGTERSAGITPDWLAIPVGNAGNITAYWKGFSEY
                     AAAGRIGALPRMAGFEAAGAAPIVLGRPVEHPETIATAIRIGNPASWASARAAADDSG
                     GFIDSVTDEEILDAYRLLAQTEGVFAEPASCACVAGLVKMARDGRLKDGQCAVAVLTG
                     NGLKDPSTAIQQAPRVRGAINADYESVSSEVLTYAGR"
     gene            2913..3845
                     /gene="thrB"
                     /locus_tag="PLM74_09495"
     CDS             2913..3845
                     /gene="thrB"
                     /locus_tag="PLM74_09495"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HON43344.1"
                     /translation="MPVDRVIVRAPATSANMGPGFDALGFAMELRDEVMFERASDGES
                     SAITVDPDGRQVSLGIPTGSDNLALRAFAAAFRDAGQAAPAVRVCIVNRIPVARGLGS
                     SASAIVAGLMAADAFMGIAPDLQRLLSTAARIEGHPDNTAACILGGITASSFVGARVL
                     WARVPVHGSLRAVVAVPDFPVSTRAARNVLPGSVPHSDAVFNVGRTALLVASLASGDY
                     ANLRYAFDDRLHQPYRSGLVPGMINAMKAACDSGAIGSYMSGAGPSIMALIDRDGLAE
                     PVAFAMREAFARVRVNALTMDLPMAESGAAFEVV"
BASE COUNT          664 a         1142 c         1368 g          872 t
ORIGIN      
        1 gggttctact tctcgcgcga ttcgtccata ctcactccgc tgcctctgcc ggcgtccact
       61 gtggtgatgg ccaggttcgg ggtagtgctg atcaatgaat acatcacgct ggcgctggtg
      121 ctagtgccgg ccttctgtgt atatggaata cgatcgggcg caggtttgct ctactggata
      181 acgtgcatac cagtgtttct gctgacgccg gtgattcccc tggctatcga cgccgcagtg
      241 gccatactgc tcatgcgttt tgtgaatgtg cgcaagaacc gaacgttgtt catgttcctt
      301 ggcacagcca tcttcctcgg gctgtatttc tggtttcagt actacatcat gcaccaggcg
      361 ccccaaagcg aggccgacat gctcaactac ctaatgactg tgcaggacag cctgtcgcgg
      421 tcgatggcca tgcgctttcc gcccagcctt tgggcttcgt tctcgctgtc gaaggcgggc
      481 actgccgctg ggctcgggaa cctggccatc ctggttgcag tgtctgccgg cgcgctcctt
      541 ctggcgttgg ctctcggttc acggctgttc tatgcagccc ttgcagccgg cggcgaggtg
      601 gcggtatccg ccgccggtcg caaagtgggg cgctggggcc gccgtcagcc gacccaaacc
      661 gccggggatt tggctctgcc cgccgcgtgg tgggtgcagc gttcgccgga ggtcgcgata
      721 gctctcaagg acatgtggct gtttgtgcgc acaccgacgt atttactgaa cggcctcgct
      781 aatgtgctgg tattcccggg cctgctagct gtgtggttca ttgcgggagg tcgaggggga
      841 gtgggagggg gcgcgggagt tggcaacccg ttcgccgaga tccccgggtt tgccgctttc
      901 atgtcatcgc ctgaatttgc agccgcccgc gcgctggcct tcgccggcgc aatcctgttt
      961 gtgtccggga tcaacgcagt ggcttcgtcc gcgttctccc gtgatgggat gcagtactgg
     1021 gtgtacaagg tgatccctgt atcgttccgg cgtcaggtga tcgggaagat catgtttgcg
     1081 ctggcgtttc agtttgcgtc catgttgccg ctcgtggtgg tgatgcagct gattctccgg
     1141 cttgacctgg cggcgcttgc ggcgggcgtg ttgatcgggg tggcggggtc gacgtgggcg
     1201 tcgctcacat cgctgtatgt cgacatgatg cgcccctacc tcacctggga caaccctcag
     1261 agggcgatga agagcaacct gaacgcactc atcgccatgg ctgcggtgac ggtcgtagca
     1321 gtgggggcgg gatacgccat tgtgaaggcg ttgtcggcag gcctggacca gcacgcggtc
     1381 atctggtgta cgttcgtgtt tttcgcggca ttgtcgattc tatcatatcg cctgctgatg
     1441 tcaggagcgg ggcgggcatt tcgccgcgtg gagttgtagg cacgtagagt tgtaggcacg
     1501 tagagttgta ggcacgtgga gttgcggccg cgtggagttg tagggtttgg gaccgcgcgg
     1561 tctgtagcta tctatgcatt acccacatcg ctgggaagga tccgagtctt ggccattgac
     1621 cgaacagctc acgcagtgag cgtgcccgcg ccagaaggtg ccggctggtg ttgataaggg
     1681 ccctgcgcag gtctttaggg aaaaccgcgt ttggcagggg gttagcgaac ctgcaagaag
     1741 tatgtacggt cttggacgga acctgttcag atacgaacaa gatgctattt gctgctggga
     1801 gtgttcgtct ttgagacttt ggagaggaat catcgaagag tacagggaat tcataccgtt
     1861 tggcgcgggc gcgcccattg tcacgttgct cgaaggcgga acgcctcttg tccctgcgcc
     1921 tgctctggcc cggcggctgg gcgttcgagc cgatatccgt cttaagctgg agggcgcgaa
     1981 ccccaccgga tcgttcaagg atcgcgggat gactgcagcg atcaccaaag cggtcgcgga
     2041 cggtgcccgc gtggtcatat gcgcttccac cggcaacact tcggcctctg ccgcggccta
     2101 cgctgcccgc gcggggcttc gctgtgttgt ggtgataccg gatggggcca tagccatggg
     2161 caagctggcg caggccattg cccacggggc cgaggtcgtg ccgatcaggg gcaacttcga
     2221 tcaggccctg gcgattgtga gggaaatggc cgatgcaggg atcgccactt tggtcaattc
     2281 ggtcaatccg tggcgcattg aaggccagaa gaccgccgct ttcgaaatag tggatcagct
     2341 cacctcgggc ggacatggcg cgccgggcac ggagcgctcg gctggcatca ccccggattg
     2401 gctggcgatt cccgtgggca atgcaggcaa catcacagcg tactggaagg gcttctcgga
     2461 gtatgccgcc gccggtcgga tcggcgcgtt gcctcggatg gctggatttg aggctgccgg
     2521 ggccgccccc atagtgctgg gcaggccggt ggagcacccg gagaccatag ccactgccat
     2581 cagaatcgga aaccctgcga gctgggcgag cgctcgggct gccgccgatg actccggagg
     2641 gtttattgac tccgtaacgg acgaggagat tctcgacgcc taccgcctgc tggcgcagac
     2701 tgaaggcgtt tttgctgaac ccgcctcgtg cgcttgtgta gccgggctcg tcaagatggc
     2761 tcgcgatggt cgcctcaaag acgggcagtg tgccgtagcc gtgctcaccg gcaacggcct
     2821 gaaggatccg tctacggcta ttcagcaggc gcctcgggtc cgcggcgcca taaacgccga
     2881 ttacgaaagc gtctcaagtg aggtgttgac gtatgccggt cgatagggtc atagttcgcg
     2941 cgccggctac ttcggccaac atggggccgg gattcgatgc tttggggttt gccatggaac
     3001 tccgtgacga ggtgatgttc gagcgcgcgt ccgacgggga gtccagcgcg atcacagttg
     3061 acccagacgg taggcaggtt tcgctgggaa tccccacagg cagcgacaac ttggcacttc
     3121 gggcatttgc ggctgcgttt cgtgacgcag gccaggcagc gccggcggtg cgggtgtgta
     3181 tcgtcaaccg catacctgtg gctcgaggcc tcggaagcag cgcgtcggcc atcgtcgcgg
     3241 ggctcatggc cgccgatgcc ttcatgggca tcgctcccga cctacagcgg ctattgagca
     3301 cggccgcccg gatcgagggg caccccgaca atactgcagc ttgcatactt ggcgggatca
     3361 ccgcgtcctc cttcgtaggg gcacgcgtgc tgtgggccag ggttccggtg cacggcagcc
     3421 tgcgcgctgt agtggccgtg cctgatttcc ccgtgtctac cagggccgcc cggaacgtgc
     3481 tgccaggcag tgtgccgcat tccgacgctg tgttcaacgt ggggcgtacg gccctgctcg
     3541 tggcctcgct ggcatcgggc gactatgcca acttgcggta tgcctttgac gaccgacttc
     3601 accaaccgta ccgctccgga ttggtgcccg gaatgatcaa cgccatgaag gcggcgtgtg
     3661 attcaggcgc gataggttcg tacatgagcg gcgcaggccc atccataatg gccctgattg
     3721 accgtgatgg gctggcggag ccagtggctt ttgcaatgag ggaggctttc gcgcgcgtca
     3781 gggtaaacgc cttaaccatg gatttgccaa tggccgaatc gggcgctgca tttgaagttg
     3841 tgtagatggc atatgaagct cgcttcagtg agagactgga tggaaccgac gggcggtgac
     3901 gcaggtggaa ttgccggcct actttggcct gggctcgaac ctcggcgata gactgggata
     3961 cctggcgcgg gcatgcgaga tgatgtacaa tgctccgggg ttcgcgcggc acgccgtgtc
     4021 gagggtatac gagactgaac cctggg
//
LOCUS       DAOTAC010000237         4024 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_18089_length_4024_cov_4.256236, whole genome shotgun sequence.
ACCESSION   DAOTAC010000237 DAOTAC010000000
VERSION     DAOTAC010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4024
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18089_length_4024_cov_4.256236"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="PLM74_09500"
     CDS             complement(<1..404)
                     /locus_tag="PLM74_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HON43345.1"
                     /translation="MSRNPMSSWSRLLGMSKSPVSSWLRRSAVAILFVALVAAAGSVH
                     AAGGYPVSIVDDTGATIEIPREPVRIVSLAPSNTEILFALGLGDKVVGVTSACDYPEV
                     AKKIDKIGDYNINIEAVVAKRPDLVVAVADLQ"
     regulatory      complement(576..840)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            852..1076
                     /locus_tag="PLM74_09505"
     CDS             852..1076
                     /locus_tag="PLM74_09505"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HON43346.1"
                     /translation="MYILAQPPILAKIAMSTGVGPRIANSSSTQSGPPGNRKAVAALI
                     EAQSLEIDTVSGATITTRAFLKSVGNALAK"
     gene            1084..2076
                     /locus_tag="PLM74_09510"
     CDS             1084..2076
                     /locus_tag="PLM74_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HON43347.1"
                     /translation="MALQHTSAESLNLGMDTVMTHRAFGRHAAEAIQAVKQEAARLEG
                     MLSRFMPESDISAINESAGHACCSVGLETYDVLSCATELSTACQGLFDVTIGPLVDLW
                     GIGHGALRPPAEAEIHRARCMVNYLDLLLDPVLRTAGLRRTGQSIDLGGIGKGYAADR
                     FIQVFREYNVSSAFTNIGGNVAALGARPDGSAWRVGIQHPRQTDLLIGLVSVKDKAVV
                     TSGDYQRFFADGEGNRYHHILDPTTGYPAKSGLTGVTVVAHNSGSGSMIADALSTAVF
                     IAGLDRGLALISGYPEVEALFVDAEPTRAGLSVWVTRGLRDCFSAAQDVSVHIV"
     gene            2098..2481
                     /locus_tag="PLM74_09515"
     CDS             2098..2481
                     /locus_tag="PLM74_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HON43348.1"
                     /translation="MLKVIMWIVAGLVILFVGAGLLDAPGRKAGERLQVGKVDFSSLK
                     DGIYEGQYMGGRWSNKVRVTVASGKVTGIGILRDVWFRKPELTEQVVSDVIKSQSLEV
                     DIVSGATVTTRAYLKAVENALAEKK"
     gene            complement(2534..>4024)
                     /locus_tag="PLM74_09520"
     CDS             complement(2534..>4024)
                     /locus_tag="PLM74_09520"
                     /inference="COORDINATES: protein motif:HMM:NF015126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SelB C-terminal domain-containing protein"
                     /protein_id="HON43349.1"
                     /translation="TGQGLDELLHQLDLALDRAASASAPERLSCTRLPIDRVFAISGH
                     GTVVTGTLIGGSIKPGDRLELLPAKKPVRARQVQVHDVPVEAAERGQRVALNLAGLER
                     GEIERGMVLATPGILEPSRLFDAELTLLTSAAPVEHGDRMHLHMGTAEATCRVRVIGG
                     DAAFPGETTFVQLELEECDGVAARGDRFIVRTYSPMSTAGGGRILAVARGRVRRFRPE
                     IVGEFETLSAGSDADLVTLFVHRALREYARPATAADVARAMFREEASVRQLLERGATT
                     GAIVGLGGSGGLGGLGSSGDSEQRGFLTSDDWNDIESRMHELLADFHAREPLRAGMAR
                     EELRTRAFPGWDSKWFTLAMSRAAADGIVREQGALAALPSHTVTLSPEQQVWAHRIED
                     AFASSLMSPPERAEAMAGGAPQAVLDYLIDTGVVMRVGPELLFHRDAIARALEFAREL
                     GRDGRAFTAAEFRDSCGNSRKYAVALLEYMDSSRITRRLGDERVLA"
BASE COUNT          766 a         1295 c         1236 g          727 t
ORIGIN      
        1 atctgcagat ctgccacagc cactaccagg tcgggtcgtt tggccacgac ggcctcgatg
       61 ttgatgttgt aatccccgat cttgtcgatt ttcttggcaa cttccgggta gtcgcatgca
      121 gatgtgaccc cgacgacttt gtcgccgagc cccagggcaa acaggatttc ggtgttgctt
      181 ggtgcaagag acacaatcct tacaggttcg cgaggaatct cgatggtggc accggtgtcg
      241 tccacgatcg aaaccggata cccgcccgcc gcgtgtaccg aacctgccgc ggccacgagc
      301 gcgacgaaca ggatcgccac agccgacctg cgcagccatg acgaaacagg agatttgctc
      361 atcccgagca gccgagacca actggacata ggattcctgg acatctcagt aacactcctt
      421 ctccggcgcg tgccgcacgg ctcttcgcgc cggcggtcgc gcacataaaa acaccctgct
      481 caccggcgag tgaacagggg agaagtcggt acggtctgcg ccccgaaaac ccggtcaacg
      541 cagcaccttc cgcatccccc ttatcctcga aggtggagtc ggcaagctcg gtgggcaggt
      601 ttcctgactt ccgggtcgtc gcctgcgcgc gtcttccccc tgccgcacgt ggcaagagtg
      661 acatcaaatg cgcctcggct caccggatac agtggcggga ccgcgggctt ggcccggcgc
      721 tgcgcgacgt cgcctcgttc gacgctcctc ctgctcggag tcacgcccga cgcgctcatg
      781 cgcccggctg ccacttccct ttcacccgct tgcgcgggca cccaccggca ttccgttatt
      841 caggtgttac tgtgtatatc ctagcacaac cgccgatttt ggccaagata gcgatgagca
      901 caggagtcgg cccgcggatc gcgaatagta gctcgacaca atccggaccg cccggcaatc
      961 gtaaggcggt cgcagcgctc attgaagccc agtcacttga gatagacact gtgagcggcg
     1021 cgaccatcac cacccgggcc tttctcaagt ccgtgggaaa tgcgctagcg aagtagaggt
     1081 gacatggcgc tgcagcacac atcagccgaa tcgctgaacc tggggatgga cacagtgatg
     1141 acccaccggg ctttcggcag gcatgccgct gaagccattc aggccgtaaa gcaggaggcc
     1201 gcgcggctcg agggcatgct gagccgtttc atgcccgaaa gcgacatatc cgcaatcaat
     1261 gagtcggcag gacatgcgtg ctgcagtgtc ggtcttgaga catacgatgt gctgtcgtgt
     1321 gcgaccgagc tgtcgacggc atgccagggg ctctttgacg tgactattgg gccgctggta
     1381 gacctgtggg gcatcggcca cggcgccctg cgccctcctg ccgaggccga gatccaccgg
     1441 gcgcgctgca tggtgaacta tctggatctg ctgctggacc cggtcctgcg gacggcgggc
     1501 ctgcgcagga cggggcagtc catcgatctc gggggcatcg gcaaaggcta cgccgccgac
     1561 aggttcatcc aggtgttcag ggagtacaac gtgtcgtcgg ccttcacaaa cataggaggc
     1621 aatgtggcgg cgctgggcgc gcggcccgac ggatccgcct ggcgcgtcgg cattcagcat
     1681 ccccggcaga cggacttgct gatcggtctg gtgtcggtga aggacaaggc ggtggtcact
     1741 tcaggcgact accagcgctt cttcgccgac ggcgaaggta acagatacca ccacatactg
     1801 gacccaacca ccgggtaccc ggccaaatca ggattgaccg gcgtaactgt ggtcgcccac
     1861 aactccggca gcggatcaat gattgccgat gcgctatcca cagcggtgtt cattgccggg
     1921 ctggacaggg ggcttgcgct gatcagtggg tatccggagg ttgaggctct cttcgtcgac
     1981 gcggagccga cccgtgcggg gctatcggtc tgggtcaccc gaggcctgag agattgtttc
     2041 tctgctgctc aagacgtgag cgtccatatt gtctagcaga caaaaggggg gaatgagatg
     2101 ctcaaggtaa tcatgtggat cgttgccgga ctagtcattc tatttgtggg agcagggctc
     2161 ctagatgcgc ccggtcgcaa ggccggcgaa aggcttcagg ttggcaaggt ggacttctcg
     2221 agtctcaaag atggcatcta cgaagggcag tacatgggtg ggcgctggtc aaacaaggtc
     2281 cgggtgacgg tagcgtcagg aaaggttacc gggatcggaa tattacgaga tgtctggttc
     2341 cgcaagcccg agctgacaga gcaagtggtt agcgacgtca tcaagtctca gtcgctggaa
     2401 gtggatatcg tgagcggcgc caccgtcacc acccgggcct acctcaaggc cgtggagaac
     2461 gcgctcgccg aaaagaagta acacctgagg gatgatgatg ctgtcgccgg ttctggcgca
     2521 gggcaagcgt ggttcaggcc agaacccgtt catcgccgag acgtctggtg atgcgcgacg
     2581 agtccatgta ctccagcagt gccactgcgt atttccgaga gttcccgcat gagtcgcgaa
     2641 actcggctgc cgtaaacgcg cggccatcgc gcccgagctc gcgagcgaat tcgagcgccc
     2701 gggcgatcgc gtcgcggtgg aagagcagct caggcccaac gcgcatcacc actcctgtgt
     2761 caatcaggta gtcgagcact gcctggggag cccctccggc catcgcctcg gctcgctcag
     2821 gcggcgacat aagagaggag gcgaacgcgt cctcaattcg gtgcgcccac acctgctgct
     2881 ccgggctgag ggtcactgtg tgagacggca gcgccgccaa ggcgccctgt tcgcgcacga
     2941 tgccgtctgc cgccgccctg gacatggcca gtgtgaacca tttggagtcc catccgggaa
     3001 atgccctggt ccgcaattcc tccctggcca tcccggccct aagaggctcg cgggcgtgga
     3061 agtctgccag cagttcatgc attcggctct cgatatcgtt ccagtcatcc gaggtcaaga
     3121 atccccgctg ctcgctgtcg cccgaactgc ccaagccgcc caggccaccc gagccgccca
     3181 gacccacaat cgcgcccgtg gtcgcccccc gttctagcag ttggcgcacg ctcgcctctt
     3241 cacggaacat ggcccgcgcc acatccgcgg cagtcgccgg gcgagcatac tcccgaagag
     3301 cacgatgcac aaacagtgtt actaggtctg cgtcgctccc cgccgataat gtttcgaact
     3361 cccccacgat ctccgggcga aaccgccgca cccggcctcg cgcaacggcc aggatccgcc
     3421 ctcccccggc tgtggacata ggcgagtagg tgcgcacgat aaaccggtcg ccccgcgccg
     3481 caacaccgtc gcactcctcc agttccagct ggacaaaggt ggtctccccg gggaaagccg
     3541 cgtcgcctcc gatcaccctg accctgcatg tggcctcggc ggtgcccata tgaagatgca
     3601 tgcggtcgcc atgctccacc ggcgccgccg acgtaagcaa cgtcagctct gcgtcgaaca
     3661 gccgcgacgg ctccaggatc cccggagtcg ccaataccat gccacgctcg atctcgcccc
     3721 gttccagccc ggccaggttg agcgcgaccc gctgcccgcg ttcggctgcc tccacgggca
     3781 cgtcatgtac ctgcacttgc cttgcccgaa cgggtttctt cgcaggaagc agttccaagc
     3841 ggtcgccagg cttgatcgag cccccaatga gggtacccgt aaccactgtg ccgtggcccg
     3901 atattgcgaa tacgcggtcg ataggaagac gggtgcagct taggcgctcg ggcgcagagg
     3961 ctgatgcggc tcggtccaaa gccaggtcca gctggtgcag tagttcatcc aggccctgtc
     4021 cggt
//
LOCUS       DAOTAC010000238         4013 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_18152_length_4013_cov_4.966902, whole genome shotgun sequence.
ACCESSION   DAOTAC010000238 DAOTAC010000000
VERSION     DAOTAC010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4013
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18152_length_4013_cov_4.966902"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      618..770
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02683"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="NiCo riboswitches sense nickel or cobalt ions;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="nickel or cobalt"
                     /db_xref="RFAM:RF02683"
     gene            867..3431
                     /locus_tag="PLM74_09525"
     CDS             867..3431
                     /locus_tag="PLM74_09525"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HON43350.1"
                     /translation="MSTAIDVKNLSGLTSEEAQRRLAAEGPNELPSGKKRSLLHIVLE
                     VVREPMFLLLVGCGALYLLLGDHQEALMLLSFVFVVMGITIYQERKTENALEALRDLS
                     SPRALVIRDGRQTRIPGREVVRGDVVLMSEGDRVPADSVLLWTMNLSVNESLLTGESL
                     PVRKSSIEGTAESTEMERPGGEDTPFVYSGTLVVQGQGVAEVKCTGARTEIGRIGKAL
                     QTVEAEETALNRETGRMVRTWAMLGFVLFAAVVIVYGLTRANWIQGLLAGITLAMAML
                     PEEFPVVLTIFLALGAWRMSQKNVLTRRTPAIETLGAATVLCVDKTGTLTQNRMSVSR
                     LLARDECYDADLGGSNLPEEFHELIEFGILASEKDPFDPMEQALRQFGQKHLVDTEHL
                     HDDWTLVQEYALSRELLALSHVWMMPNGNGGHYVVAAKGAPEAIADLCHLTPDATAAL
                     LAQVSQMAADGLRVLGVAKAKFRAAELPDEQHDFRFELVGLVGLADPARPTVWRAIEQ
                     CYQAGIRVVMITGDYPGTAQNIARQIGLSPADQVITGPELTAMSDSDLAEKVKTCCIF
                     ARMVPEQKLRLVEALKADGEIVAMTGDGVNDAPALKSAHIGIAMGGRGTDVAREAASL
                     VILDDDFSSIVEAVRQGRRIYDNLRKAMAYIFAIHVPIAGLSLLPVMFEWPLVLLPVH
                     IAFLELIIDPACSVVFEMEPEEADVMNRPPRDPSSPLFSSRLMRVGIVQGLSVLAFVM
                     AVYVVSLLVLDRGELEARSLTFTTLVLANIGLIMANRSWSGNLLAQLQRPNPAVRWVV
                     GGSLAFLAAVLSIKALRGLFHLTVLHPVDIAICLVAGVLSVLWFELFKANRGISRA"
     gene            complement(3400..>4013)
                     /locus_tag="PLM74_09530"
     CDS             complement(3400..>4013)
                     /locus_tag="PLM74_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43351.1"
                     /translation="AVWLRGARPAPALGVMRERIDSYFSSRALAMMMKASHVADGWGN
                     SCDYLNLYDQAEAALDREFGANSAMSGVSRSIIKRLDVDGIRRARRANYAELVRALCE
                     QDAIHAGVELLFPGLPDGVVPLGCPVATDERDALREHLAARGILTQILWQSPPEVPRD
                     EFPESWALSERILVLPCDQRYSPEHMEAVARAAVRRDLSPDSL"
BASE COUNT          785 a         1224 c         1232 g          772 t
ORIGIN      
        1 cgaagggcca cctgaccgcc tccaaatcac gaaaatcggg gggtatccga cgggtctggg
       61 cagtgaagca ggcgggcggg ctgacataat atcccgcatc atggctcaag ttggtaatgg
      121 cgcgcaagtg atggcgcgta gtgccatcga ttcgccaata aatcggcggt atccggcccc
      181 gatggcccct tttgcggggt cgattcgcct cccggggacc ccggcccaaa tcgatgtact
      241 caaaagttgc gtttttcgcc gaaagtattg tcaataagtg acggtatccc tgggcggata
      301 cccccggggg tatatcactg agccacagca gtcgtctttc cgtccctcag cacgcccccg
      361 gatctatccc cggacttatc cctgggccta ttcctggact tacccgcggt tctatccctg
      421 gacccctgga cccagtgcga ttccgactgc ttccgctgtg gccccggcga gaccacacgc
      481 ctgaatgcgg gggatgtgcc accccgaaaa ccacaatccc tgcacgcacc cgacggccgg
      541 acggccgaac ggtcaacggt gagcgttgac accccacctg gccggtgata acatctgtgc
      601 ctagagtgca taatgcgcca atattgacgg agcaggcgat tgactgaagc cgcgctgaac
      661 gtccgcagcc cattgcaaga gggagtgcgg cgtcaagtgc gatgctttcc gtgtcagtgg
      721 ggctgggcca gagatggcag cgggtgatta agcaagcccg tgggaccgcg acttcaagtc
      781 acggttccgc gggcttttcg cgtactctgg tatgtcggcg tatggagggc ccgacgcaac
      841 cgaacactca aagcggaggt actgccgtgt caacagccat tgatgtcaag aacctgtctg
      901 ggctgacaag cgaggaagcc cagaggcggc ttgcagccga ggggcctaat gaactacctt
      961 cgggcaagaa gcgttcgctc ctccacatcg tcctcgaggt cgttcgcgag cccatgtttc
     1021 tattgctcgt gggatgcgga gcactctatc tcctgctagg agatcatcag gaagctctga
     1081 tgctgctcag cttcgtgttc gtggtcatgg gcatcactat ctaccaggag cgaaagaccg
     1141 aaaacgcatt ggaggcccta cgtgatctgt ccagcccgcg agctctggtt atccgcgacg
     1201 gccggcagac gaggatcccg ggacgcgaag ttgtgcgcgg cgatgtcgtg ctgatgtcgg
     1261 aaggcgaccg agtgccggcc gattccgtgc tgctgtggac tatgaacctc tcggtcaacg
     1321 agtcgctgct caccggcgaa tcgctccccg tgcggaagtc gtcgatcgaa ggaactgcag
     1381 aatctacaga gatggaacga ccgggcggcg aagatacgcc cttcgtgtac tcaggaaccc
     1441 tggtcgttca gggccaaggc gtagcggagg tcaagtgcac cggggccaga accgaaatcg
     1501 gtcgcatagg caaggcatta caaacagtgg aggcagagga gaccgcccta aaccgcgaga
     1561 ccgggcgcat ggttcgcacc tgggccatgc tcggctttgt gctcttcgca gcagtagtca
     1621 tcgtgtatgg gctcactcgg gccaactgga tccagggcct tctggcaggg atcacgcttg
     1681 ccatggcgat gctgcctgag gaattccctg tagtcctcac aatcttcctg gcactgggcg
     1741 catggaggat gtcgcagaag aacgtgctca ctcgcagaac tcccgccatt gaaaccctcg
     1801 gggcggccac agtgctgtgc gtcgacaaga ccggcaccct tacgcagaac cggatgtcgg
     1861 tgagcaggtt gcttgcacga gacgagtgct atgacgccga tttgggcggg tcgaatcttc
     1921 ccgaggagtt ccacgagctg atcgagttcg gaatactggc cagcgagaag gacccgttcg
     1981 accccatgga acaggcgctg cgccagttcg gacagaagca tctggtggac acagaacacc
     2041 tgcacgacga ctggacattg gtccaagagt atgcgctctc acgtgagctc ctggcgctgt
     2101 cgcatgtgtg gatgatgcca aacggcaacg gcggccatta cgttgtggcg gccaaaggcg
     2161 cgcccgaagc catcgccgac ttgtgccacc tgacgcccga cgccaccgcg gcacttctcg
     2221 cccaggtaag ccagatggcc gccgacggcc ttcgggtgct gggcgtggcc aaggcgaaat
     2281 tccgggcggc tgagcttcct gacgagcagc acgacttccg attcgagcta gtcggactcg
     2341 taggactggc cgaccctgcc aggcccacag tttggagggc aatcgaacaa tgctaccagg
     2401 cggggatcag ggtggttatg ataacaggcg actaccccgg cactgcccag aacatcgcgc
     2461 gccagatcgg cctgtcgccg gcggaccaag taatcacagg tcccgagctt accgcaatgt
     2521 ccgactcaga tctggccgag aaggtcaaga cctgctgcat cttcgcgcgc atggtgcccg
     2581 agcagaagtt gcgtctggtc gaagccctga aggcggacgg agaaatcgtg gccatgaccg
     2641 gcgacggcgt caacgacgcc ccggccctaa agtccgcaca tatcggcatt gccatgggcg
     2701 gtcgcggcac cgacgtcgcg cgcgaagcgg cctcgctcgt gatcctcgac gacgatttct
     2761 cttcaattgt cgaggccgtg cgtcagggca ggaggatata cgacaacttg cgcaaggcga
     2821 tggcgtacat cttcgccatt catgttccca ttgccgggct gtcgctgctc ccagtgatgt
     2881 tcgagtggcc gctggtgctg ctgccggtgc acatcgcctt cctcgaactc ataatcgacc
     2941 cggcatgttc agtggtgttc gagatggaac cggaagaagc agatgtgatg aacaggcctc
     3001 ctcgtgaccc atcaagcccg ctcttcagca gcaggcttat gcgcgtgggg atcgtccagg
     3061 gcctctccgt gctggcattc gtaatggctg tgtacgtcgt ctcattgctg gtcctagacc
     3121 gtggagaact ggaagcgcgc agcctcacgt tcacgactct ggtcttggcc aacatagggc
     3181 tgatcatggc caaccgatcg tggagcggca atctcctcgc ccagttgcaa aggcccaatc
     3241 ctgccgttcg gtgggtggtg ggcggttcgc tcgccttcct ggcggcagtg ctgagcatca
     3301 aggcgctgcg cggccttttc catctgaccg tgctgcaccc agtcgacatc gcgatctgcc
     3361 tggtcgcagg agtgctgagc gtgctctggt ttgagctgtt caaagcgaat cgggggataa
     3421 gtcgcgcctg accgccgcgc gcgccactgc ctccatgtgt tcgggcgagt atcgctgatc
     3481 gcacggaagc acgagtatgc gctctgatag tgcccagctc tccgggaatt cgtcccgcgg
     3541 tacctcaggc gggctctgcc agaggatctg ggtgagtatg ccccttgccg cgaggtgttc
     3601 ccggagtgcg tcgcgctcat ccgtggccac agggcatccc agcggcacga ccccgtccgg
     3661 aagtcctgga aagagcaact caaccccggc gtggatcgcg tcctgttcac agagtgctct
     3721 cactagctcg gcgtagtttg cccgccgggc ccgtcggatt ccatccacgt ccagccgctt
     3781 gataatggat ctggaaacgc ccgacatcgc gctattcgcc ccgaactccc tgtcgagcgc
     3841 ggcctcagcc tggtcatata ggttgaggta gtcacacgag ttgccccagc catctgctac
     3901 atgcgaagcc ttcatcatca tcgccagggc cctggagctg aagtaggagt ctatccgctc
     3961 tctcatgaca cctagagcag gcgcaggccg agcgcctcta agccacaccg cag
//
LOCUS       DAOTAC010000239         3985 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_18296_length_3985_cov_3.544275, whole genome shotgun sequence.
ACCESSION   DAOTAC010000239 DAOTAC010000000
VERSION     DAOTAC010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3985
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18296_length_3985_cov_3.544275"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            223..1302
                     /locus_tag="PLM74_09535"
     CDS             223..1302
                     /locus_tag="PLM74_09535"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HON43352.1"
                     /translation="MLKKKLVYALCAFIASCSVCLLLAGMEVAASAILTFTEKSIQAT
                     SAFRLLKDLCSHSHTVLLARFGADSEVVADWNEGLLAKGLPRSGSSLLTLATGPDGLL
                     DAVRALKDQCIPARKAVGAVVSERKGSTSSNGIRMIEHKVAKGESLSVIAERYAVSVD
                     TIMWSNSLSNPNRLKIGQTLKFPSISGVVHLVTRGESIWTIAKKYGVSRDEIVRTNQL
                     DEPDKLKLKQALVIPGAKPLPVRNSPVAVASRGAASSRSSGSSSGDALIWPLSGRISS
                     RFGFRWGRMHNGLDIAVPIGTPVKAAADGCVIFAGWRSGYGRLVILDHGAGVHTYYGH
                     NSSFTVGAGDAVAGGPDCNPGDLML"
     gene            1450..2709
                     /locus_tag="PLM74_09540"
     CDS             1450..2709
                     /locus_tag="PLM74_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012813476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HON43353.1"
                     /translation="MAASDRSGRVMRNDNARAVARAGNYFVELQPSDVIPLPAGASLA
                     RLPERVPVGADASSGEFVQAGPGEAVAAILPQGYTRTFVPAYVSKPNAPTLPLFGYSA
                     AAFDGDQMVVAAMRTDERDTWDPRHYNLPELQELVEAARGAHPGNRILRQLARCAMEY
                     QCYTAQNLFYRRWEAGIPVSPTCNARCLGCISEQPAECCPSPQQRIDFVPTIDEIVEL
                     ALPHLEEAPQAIISFGQGCEGEPTNEWRVIAEACRAVRARTDRGTININTNAGNTQAV
                     EALCDAGIDSFRVSLLSARPMPYAQYHRPLEHRLADVERSIAAARSRGAFVSLNYLTF
                     PGFTDLEPEVDAVCSLIERTGVQMVQFRNLNIDPEVFSSVMLERPGKPGHRDEVAEPA
                     GIAQVIAHLRDCFPDLMIGNFSVALRD"
     gene            2846..3322
                     /locus_tag="PLM74_09545"
     CDS             2846..3322
                     /locus_tag="PLM74_09545"
                     /inference="COORDINATES: protein motif:HMM:NF017648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HON43354.1"
                     /translation="MASLSDYIESYLKLLLSSMPNDWVEIRRRDLAERFRCAPSQISY
                     VLTTRFSIERGYVVESRRGGGGYIRIFRLGAAPGSDALEIVREAVGDAIDRKAADDLI
                     ARLSELGAISEQDAAFVRAAVLEGDDDRRSPELERIIRARVLKATLLFVLTQSTTE"
     gene            3347..3982
                     /locus_tag="PLM74_09550"
     CDS             3347..3982
                     /locus_tag="PLM74_09550"
                     /inference="COORDINATES: protein motif:HMM:NF014232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HON43355.1"
                     /translation="MLCQVCNQRPANVHVTKVINNVRTELHLCQQCARERGELEVFAG
                     PKFAFSNLLAGLLQSDAAAGVPAGIPGGSRSVCATCGLEYPEFMNTGYLGCSECYSQF
                     LPMLRPLIARIHGHARHTGKVPVRAGKAVRFRKELESLREELRTMIEAEEYEKAALVR
                     DKIRDLEKAAEQARQAQAEQAQTEQAQAEQDEPEQGGMEQGEVNEEEGEGR"
BASE COUNT          819 a         1137 c         1245 g          784 t
ORIGIN      
        1 ccaacatggg ctcggccgag atgacccagc tgctgataga tcggattcag cacaccaact
       61 cgaaccacga cctcatcaac acaatactca agtcaaagtt tgccgagaat cttcggagct
      121 ggcagaattc gaactgagtg accatacgtg gtcatggtgg tatactgtag tagatgccag
      181 gcttaccccg ggctggcgcc ttgagtggat ggggggagat agttgctgaa gaagaagcta
      241 gtctacgcgc tgtgtgcctt tatcgcgtcg tgttctgtat gcctgctgct agcaggaatg
      301 gaagtcgctg caagcgccat cttaacattc accgagaagt ccatccaggc aacgtcggct
      361 tttagactgc tcaaagatct ttgttcgcac agccacacgg ttctcctggc gcgtttcgga
      421 gcggactccg aggtagtcgc cgactggaac gaagggctgc ttgcgaaggg attgccccgc
      481 tcgggttcga gtctgctcac actggcgacc gggccggacg ggctgctgga cgccgtgcgc
      541 gcgttgaagg accaatgcat accggcgagg aaagctgtgg gcgccgtggt ctccgagcgc
      601 aaaggctcca cgtcaagcaa tgggatccga atgatcgagc acaaggtagc aaagggcgaa
      661 agcttatcgg tgatagccga aagatacgcc gtgagcgttg ataccataat gtggtccaat
      721 tccctatcga atccgaacag actcaagatc gggcagaccc tcaagtttcc ctccatatca
      781 ggcgtggtcc acctagtgac ccggggcgaa tcgatttgga caatcgccaa gaaatacggg
      841 gtcagcaggg acgagattgt ccgcaccaac caactggacg agcccgacaa gctcaaactc
      901 aagcaagccc tggtcatacc gggcgcgaaa ccgcttcctg ttcgcaactc tcctgttgcc
      961 gtggcgtcta ggggcgccgc tagctcgagg tcgtctggat cgtcttcagg cgacgctctg
     1021 atctggccgc tttctggccg catatcctcg cgcttcggct tcaggtgggg caggatgcac
     1081 aacggcctag acatagccgt ccccataggc acgcctgtga aggccgctgc cgacggctgc
     1141 gtgatcttcg ccgggtggag gtcgggatac ggtaggctgg tcatcttgga ccacggcgcc
     1201 ggcgtccata catattacgg ccataactcc tctttcactg taggcgcggg cgatgccgtg
     1261 gctggcgggc ctgattgcaa tcctggcgat ctgatgctgt agccgcgggc gaccggatcg
     1321 ccctgtccgg caattccggt gtcagcacag gtccgcacct ccatttcgag gtcagggtga
     1381 acgggcgggc ccgtaacccc ctcgactacc tcaggtaggg cacagatggg ggagtcagcc
     1441 gtgttgacgg tggccgcgag cgaccgatcg ggccgggtta tgcgaaatga caatgcgcgg
     1501 gcggtagccc gcgccggaaa ctacttcgtc gagcttcagc cgtccgacgt tatcccgctt
     1561 cctgcgggtg cgagcctcgc gcggttgcct gagcgcgtcc cggtgggcgc cgacgcctca
     1621 tcaggcgagt tcgtgcaggc cggcccggga gaggcggtgg cggccatact tccccagggc
     1681 tatactcgca ctttcgttcc ggcgtacgtg tcgaagccca atgctcctac actcccgctt
     1741 ttcggctatt cggcagcggc ctttgacggc gaccagatgg tagtggccgc gatgcgcacc
     1801 gatgagcgcg atacctggga cccgcgtcat tacaacctgc ctgagctcca ggagctggtt
     1861 gaggctgcgc gcggggctca tcccggcaac agaatcctgc ggcaactggc cagatgcgcc
     1921 atggagtatc aatgctatac agctcagaac ctcttctaca ggcggtggga ggccggcata
     1981 ccggtttcac ccacatgcaa cgcacgttgc ctcggctgca tctccgagca acccgccgag
     2041 tgctgcccat ctccccagca gcgcatagac ttcgtgccca caatcgacga gatagtggaa
     2101 ctcgccctac ctcaccttga ggaggcgcct caggcgatta tcagcttcgg tcagggctgc
     2161 gaaggggagc ccacaaatga gtggcgagtt attgctgaag cctgccgcgc cgtgcgggca
     2221 cgcaccgaca ggggcactat caacatcaac actaatgccg gcaacacgca ggccgtagag
     2281 gccctgtgcg acgccgggat tgattccttc cgcgtcagcc tgctcagcgc gcgcccgatg
     2341 ccgtacgcac aataccatcg tcccctagaa caccggttgg ccgacgtgga gcgctccatt
     2401 gccgccgcgc gaagccgcgg agcatttgtg tccctcaact acctgacgtt cccagggttc
     2461 actgacctcg aacctgaggt agacgcagtg tgctctttga ttgagcgcac gggcgtccag
     2521 atggtgcagt tccgcaacct caatatcgat cctgaggtct tttctagtgt aatgctggaa
     2581 aggccgggca agccgggtca ccgcgatgag gtcgcagaac ctgcgggcat agctcaggtc
     2641 atagcccacc tacgggactg cttccctgac ttgatgatcg gcaacttcag tgttgcgctg
     2701 cgtgactgac gtgtctgaac gcggctgaac cgttgtctgt ccttatttga ccaatagaca
     2761 gacccgaacg ggcttgcgtt ttcgcgcgct tggtgtatca tgatatacga aaggtcagta
     2821 atggtcagac cgttgggaga gtgccatggc gagcctgtcg gactacattg aaagctacct
     2881 caaacttctc ctaagcagta tgcccaatga ttgggtggag atacgcaggc gcgatctggc
     2941 ggagcggttc cggtgcgctc catcgcagat cagctacgtc ttgaccactc gtttcagcat
     3001 cgaacgagga tatgtcgtag agtcgaggcg gggaggggga ggctatatac gcatattcag
     3061 gcttggggcg gcgcccggaa gcgacgctct tgagatagtc cgcgaagctg tgggcgatgc
     3121 catagaccgg aaagccgcgg acgatctcat tgcccgtctg tctgagttgg gagcgatcag
     3181 tgaacaggac gcggcttttg tgcgagccgc ggtgcttgag ggcgacgacg atcggcggtc
     3241 gccggagctt gagagaatca tccgcgcgcg tgtcctcaag gcaactcttc tgtttgtcct
     3301 gacgcagtct actaccgagt gagggttttg gggggaagct atcaccatgc tttgtcaggt
     3361 gtgcaatcag aggcctgcaa atgtacatgt caccaaggtc ataaacaacg tccgtaccga
     3421 actccacctg tgtcagcaat gtgccagaga gcgcggggag ctggaagtgt tcgcagggcc
     3481 gaagttcgcg ttttctaatc tgcttgcggg cttgcttcag agcgacgcgg cggcaggagt
     3541 ccctgcggga ataccggggg gctctcgcag tgtgtgcgca acgtgcggcc tcgaataccc
     3601 ggagttcatg aacaccggtt acctaggatg ctccgagtgt tactcgcagt ttctgccgat
     3661 gctcaggccc ctgatcgcgc gcattcatgg gcatgcgcgt catacaggca aggtgcctgt
     3721 gcgtgccgga aaggccgtac gctttcgcaa agagcttgag tctcttcgtg aggaactgcg
     3781 caccatgatt gaagcggaag aatacgaaaa ggcagctctg gtgcgcgaca agattcgcga
     3841 tctggaaaag gccgctgagc aggcgaggca ggcgcaggca gaacaggccc agacagaaca
     3901 ggcgcaggca gaacaggatg agccggaaca gggcgggatg gaacagggcg aggtcaatga
     3961 agaggaaggg gaagggaggt agccc
//
LOCUS       DAOTAC010000240         3980 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_18327_length_3980_cov_4.189809, whole genome shotgun sequence.
ACCESSION   DAOTAC010000240 DAOTAC010000000
VERSION     DAOTAC010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3980
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18327_length_3980_cov_4.189809"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /gene="dusB"
                     /locus_tag="PLM74_09555"
     CDS             <1..774
                     /gene="dusB"
                     /locus_tag="PLM74_09555"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HON43356.1"
                     /translation="VQLFGSDPDVMARAASIVERLGADILDINMGCPAPKIVRSGEGA
                     ALMCRPELAWEIVRAVRAACDMPVTVKIRKGWDEETANAVEFAMGCVDAGADAVAVHG
                     RTRAQGYSGAADWRVIARVARAVPVPVLGNGDVDSPEGAARMLDETGCAAVMVGRGAL
                     GNPWIFRRALNFISTGQLLPEPGPREKIAMAMRHLADVAAFKGDETAASQMRKHLAWY
                     IRGLRGSARVREAIMAAPTVSALQGVLAEYLLVLDADQC"
     gene            827..1363
                     /locus_tag="PLM74_09560"
     CDS             827..1363
                     /locus_tag="PLM74_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006522912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HON43357.1"
                     /translation="MALVRVGDKVIDRDRIDRVLDRLFELRQNGLSQAEAAARVGIDR
                     SFVSRVENLGEVRKGKRVALIGFPVGNKAEIEAVAQSAGVDYTWLMNDEERWAYVQNR
                     SGIELLNDVVREISGFQQYDQVIFLGSDFRVRLIEAILGDRVTSVVIGRSPIHGDVEV
                     DPHDISAVIAAVRGGADQ"
     gene            1360..2280
                     /locus_tag="PLM74_09565"
     CDS             1360..2280
                     /locus_tag="PLM74_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate 5-dehydrogenase"
                     /protein_id="HON43358.1"
                     /translation="MKHIVSVSIGSSERNHSAHVSMLGEDYLVERIGTDGDIDRAIDL
                     IREMDGKVDAFGMGGIDLYMAGVGKKYVFRDAKKIARAAQKTPLVDGTGLKSTLEYRV
                     VKYAAEVEKLPIAGKPVLVVCAIDRIGMGVAFEELGCRVTYGDLIYALGIPIPVHSLK
                     WIYRLAPIIMPIATQLPFEWLYPTGKGQTTQDPKATAKHARLYADSDVIAGDFHFIRK
                     YLPDRIDGKIIVTNTVTSKDVEELGRRGASVLVTTTPEIEGRSFGTNVLEAILVTLIK
                     KPVDDITEKDYFDMLDKLEIKPRVVRYSQG"
     gene            2535..3011
                     /gene="greA"
                     /locus_tag="PLM74_09570"
     CDS             2535..3011
                     /gene="greA"
                     /locus_tag="PLM74_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003333840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HON43359.1"
                     /translation="MPEKEVILTPEGLMNLEKELEYLRTTKRREVADRIKQAIEFGDI
                     TDNSEYEDAKNEQAFVEGRIATLEKMLRNARLIEGPDGDHDEVAVGSRVVLKDLDLGD
                     VEEYTIVGSAEANPSKRRISNESPVGRAVLGKAVGSVVEVLVPAGTVKYQVLQIAK"
     gene            3022..>3980
                     /locus_tag="PLM74_09575"
     CDS             3022..>3980
                     /locus_tag="PLM74_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HON43360.1"
                     /translation="MDGLEETADVATIVDETDQRRAKLEYWRSHGVDPFGSRFVRTHS
                     SRQIIDGFADLEGKPVSIAGRVMAVRSHGKASFFDLADLSGRIQVYAKHDALGDEQYG
                     MLQQVDIGDIVGVAGDVFRTRRGEISVSLSSFTMLSKSLRPLPEKWHGLKDVDIRYRQ
                     RYVDLIVNPEVRDTFIMRTRIVSAIRSFLDKRGFLEVETPSMHAIAGGATARPFITHH
                     NALDMDLYLRIATELHLKRLIVGGLERVYEIGRIFRNEGISTRHNPEFTSVELYQAYG
                     DYHDMMEITENLVAYAAQQALGTTHITYQGSAIDLTPPWPRIT"
BASE COUNT          853 a         1066 c         1284 g          777 t
ORIGIN      
        1 gtacagctgt tcggatcaga tccggatgtg atggcgcgtg cagcctccat tgtggaacgg
       61 ctgggcgccg acatcctgga cataaacatg ggctgtccgg cgccgaaaat cgtgcgcagt
      121 ggggaaggcg cagcgctcat gtgccgacct gaactggcat gggagattgt gcgtgccgtg
      181 cgggcggcgt gcgacatgcc ggtaaccgtc aagatccgca aaggatggga tgaggagacg
      241 gccaatgcag tggagttcgc catgggctgc gtggatgcgg gcgccgatgc cgtggcggta
      301 cacgggcgga ccagggccca gggctacagc ggcgccgcag actggcgtgt aatcgcgcgc
      361 gtggcaaggg cagtgcctgt gcctgttctg ggcaacggag acgtcgattc gccggagggg
      421 gctgccagga tgctggacga gacgggctgc gcggctgtga tggtgggccg cggggctctg
      481 ggcaacccgt ggatcttccg tcgcgccctg aatttcatct ctacggggca acttctgcct
      541 gaaccgggcc ctcgcgaaaa aatagccatg gccatgcggc atctagcgga cgttgctgcc
      601 ttcaaaggcg atgagacggc agccagccag atgcggaaac accttgcctg gtacatacga
      661 gggttgcggg gttcggcgcg ggtgcgcgag gccatcatgg ccgcgcctac ggtgagtgcc
      721 ctgcaaggcg tgcttgcaga gtatctgctt gtgctggatg ccgaccaatg ctagaatcgg
      781 agtgtgcaca gttttgtgaa cataacctgg agggaaggtg gccttgatgg ctctggtccg
      841 cgtaggagat aaggtcatcg accgcgacag aatcgaccgc gtccttgacc ggctgttcga
      901 actgcggcag aacgggcttt cccaggcaga agcggctgct cgagtgggca tcgacagatc
      961 cttcgtttcg cgtgttgaga acctgggcga agtgcgcaag gggaagcggg tcgccctgat
     1021 cggtttcccg gtcggcaaca aggccgagat cgaggcggtt gctcaaagcg caggcgttga
     1081 ctacacgtgg ctgatgaatg acgaggagcg ttgggcatac gtccagaacc ggtcgggcat
     1141 tgaactgctg aacgatgtgg tacgggagat atccggcttc cagcagtatg accaggtcat
     1201 cttcttgggc tcggactttc gggtacgtct tatcgaagcc atcctgggtg acagagtcac
     1261 cagcgtagtg attgggcgat cgcccatcca tggggatgtc gaggtcgatc ctcacgacat
     1321 ttcggccgtc atagcggccg tccgaggagg cgctgaccag tgaagcatat cgtgagtgtg
     1381 agcattggct cgtcagagcg taaccactcg gcccacgtca gcatgctggg cgaggactat
     1441 ctggttgagc ggatcggaac agacggcgac atagaccggg ccatcgatct catacgtgag
     1501 atggacggca aagtggacgc gtttggaatg ggcgggatag acctatacat ggcgggcgtc
     1561 ggcaagaaat acgtcttcag ggatgcgaag aagattgctc gcgctgcgca gaaaacgccg
     1621 ctggttgacg gcacggggct gaagagcacg ctggagtaca gagtagtaaa gtacgccgcc
     1681 gaggtggaga aactgcccat agcaggtaaa cccgtgcttg tggtgtgcgc gatcgacaga
     1741 atcggcatgg gagtggcctt cgaggaactt ggttgccgcg ttacctacgg cgacctgata
     1801 tacgccctcg gcatacccat tccggttcat tcactgaagt ggatataccg cttggcgccg
     1861 atcatcatgc cgatagccac tcaactgccg ttcgagtggc tgtacccgac aggcaaaggt
     1921 cagaccacgc aggatccgaa ggcgacggcc aaacatgcga ggttgtatgc agactccgac
     1981 gtcattgcgg gcgatttcca tttcattcgc aagtaccttc ccgatcgaat cgacggcaag
     2041 atcatagtca ccaacaccgt tacctccaag gatgttgagg aactgggtag gcgcggcgcc
     2101 agtgtgctgg ttacgactac ccctgagatc gaggggcgtt ccttcggcac aaacgttctg
     2161 gaggctatcc tagtcacgct gatcaagaaa cccgtcgacg atattacgga gaaggactac
     2221 ttcgacatgc tggataagct cgagatcaaa cctcgggtag tcaggtactc gcaaggctag
     2281 gcccgccgat cgcgcgggct gatggggcga atgacatcga tagcgctttt tgcgaggcaa
     2341 gctgaccggc ttgacaacca ggggtcagct tgccataata ttggcgtgac ttgtggcggg
     2401 tgcgccatct ttttttccct atgcggggca tgcggacctc acaagctcga atccaggcgc
     2461 atgggcgcga atagaatggg taacgctccc gggcggcgtg ctcggagatg atgttcgagg
     2521 aggagcggac ttagatgcct gagaaggaag ttatcttgac tcctgaaggg ctgatgaacc
     2581 ttgagaagga gctcgagtac ctccggacga ccaagcgccg cgaggtcgca gaccggatca
     2641 agcaggccat agagtttggc gacatcactg acaactcgga gtatgaggac gccaagaacg
     2701 agcaggcatt cgtggagggc cgcatagcga cgctcgagaa gatgctcagg aatgccaggc
     2761 tcattgaagg acctgacggc gaccatgacg aagttgctgt aggatcccgc gtagtcttga
     2821 aggatcttga ccttggtgat gtcgaggaat acacgatcgt aggttctgcc gaggctaacc
     2881 ccagcaaacg gcggatatcc aacgagtccc ccgttggccg cgcagtgcta ggcaaggcgg
     2941 tggggagcgt tgtggaggtg cttgtgccgg cggggaccgt caagtaccag gtattacaga
     3001 ttgcaaagtg agtgttgatt catggacgga cttgaagaga cagccgacgt cgccactatc
     3061 gtagatgaaa cggaccagcg tcgagccaag cttgagtact ggcggtcgca cggggtggac
     3121 ccgttcggct ccaggttcgt caggactcat tcgtctcgcc agattatcga cgggtttgcc
     3181 gatcttgagg gcaagcctgt atccatcgct ggaagggtca tggccgtcag aagccacggc
     3241 aaggcctcct tcttcgacct ggccgaccta tcagggcgca tacaggtgta tgccaagcat
     3301 gacgcgctcg gggacgagca gtacggaatg cttcagcagg tcgatatcgg cgacatcgtc
     3361 ggcgtcgccg gtgatgtctt ccgcaccagg cgcggtgaga tatcggtctc cctttcctcg
     3421 ttcacaatgc tatccaagtc gctcaggcct ctcccggaga aatggcatgg cctcaaggat
     3481 gtcgatatcc ggtacaggca gcgttacgtg gacctcatag tcaaccctga ggtgcgtgac
     3541 accttcatca tgaggactcg catcgtctcc gccatccgta gcttccttga taagcgaggg
     3601 ttcctcgagg tagagacccc atccatgcac gcaatcgccg gcggtgcaac tgctcggccg
     3661 ttcattacgc accacaacgc gttggacatg gacctctacc tccgaatagc cactgagctt
     3721 cacctgaagc gcctgatagt aggcgggctg gagcgtgtgt acgagatagg tcgcatcttc
     3781 cgaaacgagg gcatctccac caggcacaac cctgagttca catcggttga gctgtaccag
     3841 gcctatggtg actaccacga catgatggaa atcaccgaga acctggtggc gtacgctgct
     3901 cagcaggccc tgggcacaac gcacatcact taccagggca gcgccatcga ccttactcct
     3961 ccgtggccgc ggatcacaat
//
LOCUS       DAOTAC010000241         3979 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_18334_length_3979_cov_4.412844, whole genome shotgun sequence.
ACCESSION   DAOTAC010000241 DAOTAC010000000
VERSION     DAOTAC010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3979
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18334_length_3979_cov_4.412844"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..433
                     /locus_tag="PLM74_09580"
     CDS             155..433
                     /locus_tag="PLM74_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008507425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HON43361.1"
                     /translation="MKHNTYFNGRVQSLALVVDGVDATVGVMEPGKYTFSTSSEEHMT
                     ITAGVLRVQLPGHSEWRDYAAGETFVVAPGKSFEVEAKEDVAYICLYK"
     gene            complement(506..2515)
                     /locus_tag="PLM74_09585"
     CDS             complement(506..2515)
                     /locus_tag="PLM74_09585"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HON43362.1"
                     /translation="MCATRRRRSQVQFRAAAACVLAVALGATCSGAPSSSAAPQSVFD
                     VYRIGGDEAFPPFEYVADSGAYRGFNVDLMNAIGIELGIQIELIPMPWSHVRRALEDG
                     DICAIQGMKLTPARQSQFLFSDAYLESSMAIFVRATEFGIAGLDDLVGRTVSVQRADA
                     GHEKLSALGGIELLTMPSQEAALVSVVAGSADAYVGNRLAGIHMAQKRGLAEYVKITG
                     DTIDPTPYAMAFRKTDQELAALFNAGIAKVKASGTYDKIHAKWFGRPIEGFWGLDRAR
                     VRWLLTASAFILAGAVLIVAWNLSLRRLVAAKTTEIAQVNALNQQILESSCDGIAAVD
                     RELNFIWANGTAMRLMGWHRGSVVGVNLSDTLLAPLAFRTPLRRIASDEAEATRCDAE
                     LGIGDEQQVFNVSLMPLMPQASSARSGALLLFSDVTALRRSEQTASTGLVMSALARSI
                     SGVAAAIRNPLLSIREYLGQLPDRYDQPEFIDEICRFVPSQVDMVNAAVGDLAFLARP
                     EPPEPRVFDLAVCAQEALARVSNEADSRGIIIRLHLADAKAFADEAQIADAVYRVVSN
                     AVACSRYRSEIEVHLRISDEAFAEVEVCGHLSDLGAQHARVEHGEQNRADTATELFFS
                     AGPGDSALGMAVAYRIIQLNGGLLTADATPDGACVKMTVPRGRQG"
     gene            2762..3448
                     /locus_tag="PLM74_09590"
     CDS             2762..3448
                     /locus_tag="PLM74_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON43363.1"
                     /translation="MPLIEARSLTREYSHGRMTVPALRGVDIEVDIGEFTAIAGPSGS
                     GKTTLLNLIGCLDRPTRGTLVIDGDDVAHYTPSMLASLRREKIGFVFQTYNLVPVLTA
                     YENVELPLVLLGRHTHSEIRDRVMSMLAEVGLAGLENRRPMDLSGGQQQRVAIARALV
                     KQPRLVLADEPTANLDSENSGAILALMKTMNETRGATFLFSSHDPLVIGYARRLVQMR
                     DGKIALDERR"
     gene            3454..>3979
                     /locus_tag="PLM74_09595"
     CDS             3454..>3979
                     /locus_tag="PLM74_09595"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HON43364.1"
                     /translation="MLRFYLGLAAKNMWRRKRRTILTAAAIAVGIMYFVMFDSLLAGA
                     DTDAELNTIDFETGHLWVSASEEGNAALAALERPTLEKTIPSAQALAQAIAELSGVAG
                     VAPRLVFPASLIAGLDELHVSAIGVNPEADSQVFRVSRFVSSGRWLEPGEDGIVLGKR
                     IADLLQLEPGDTVTV"
BASE COUNT          829 a         1203 c         1140 g          807 t
ORIGIN      
        1 tgggctttcg gtctcgggac gcgcctgacc cgcccagtgt ctgaaagggc agcgggactg
       61 tgatggagga gaatggcgtt tggaggagta tggcggcgca tgtcgaattt gtcgttactg
      121 gcaagatggg ccatatctaa gggaggagat catattgaag cacaatacct acttcaacgg
      181 gcgagttcag agcctggcat tggttgtcga cggcgtggat gctaccgttg gggtgatgga
      241 acccggcaag tacaccttct ccacctcgtc tgaagagcac atgaccatta ccgccggggt
      301 gctacgcgtt cagcttcccg gccactcgga atggcgcgac tatgctgcgg gcgaaacctt
      361 cgtggtggcg cccgggaagt ccttcgaagt cgaggccaaa gaagacgtgg cgtacatctg
      421 cctgtacaag taggcatagc ctagtcatag cagcagctga cgctacatgg caggcttcaa
      481 ggcagtgtcg gcttgttgag actcgtcagc cttggcgccc gcgcggaacc gtcattttca
      541 cgcacgctcc gtcgggcgta gcgtctgcag tgagcaatcc cccgttgagt tggattattc
      601 tgtacgccac tgccatacct agagcggaat cgcctgggcc ggcgctgaag aacagctcag
      661 ttgcagtgtc tgcgcggttc tgctccccgt gttctactcg agcatgctgt gcgccgagat
      721 cggaaagatg gccgcatacc tctacctcag caaatgcctc gtcggatatc cgcagatgca
      781 cctcaatttc tgacctatac cttgagcacg ctacggcgtt tgacactacc ctgtacacag
      841 catccgctat ctgagcctca tcagcaaagg ccttcgcatc ggcgaggtgc aagcggataa
      901 tgatgcctct ggagtccgct tcgttgctta cgcgagccaa tgcttcttgt gcgcacaccg
      961 ccagatcgaa gaccctgggc tcgggtggtt caggcctggc gaggaaggcc aggtcgccaa
     1021 cggcggcgtt gaccatgtcg acttgcgacg gtacgaacct gcatatctca tcgatgaact
     1081 caggctgatc atagcgatcg ggcaattgcc ccaagtactc gcgtatcgac agcagcgggt
     1141 tacgaatggc ggcagccacg cccgaaatcg acctggccag cgcgctcatg actaggccgg
     1201 ttgacgcggt ctgctctgaa cgcctcagcg ccgtcacatc ggaaaacagc agaagggctc
     1261 cacttcgagc agatgatgcc tgaggcatca gcggcatcaa gctaacattg aaaacctgct
     1321 gctcatcgcc tatgcccagc tcggcgtcgc atcttgtcgc ctcggcttca tctgaagcga
     1381 tccggcgcag aggtgttcgg aaggccaagg gagctaggag agtgtctgac aggttcaccc
     1441 ccacaacaga accacgatgc cagcccatga ggcgcattgc cgtgccgttg gcccatatga
     1501 agttgagctc tcggtcgacg gcggcaatcc cgtcgcagga gctttccagg atctgctggt
     1561 ttaaggcgtt gacctgtgcg atttccgtgg tcttggcagc caccagcctg cgaagtgaga
     1621 ggttccatgc tacgatgagg actgcccctg ccaatatgaa cgccgaggcg gtcagcagcc
     1681 aacgaacgcg agccctgtcc aacccccaaa aaccttcaat gggccggcca aaccacttgg
     1741 catgtatctt gtcgtacgtg cctgatgcct tgacttttgc tatccccgca ttgaacagtg
     1801 cggccagttc ctgatcggtc ttcctaaacg ccatggcata tggcgtcgga tcgatggtat
     1861 cgccggtgat cttgacgtac tctgccaagc cacgcttctg cgccatatgt atgcctgcca
     1921 gcctattgcc gacataggca tcggcgctgc ccgccacgac tgaaacgagc gcggcttcct
     1981 gcgagggcat ggtcagaagc tcaatacctc cgagcgccga cagcttctca tgccctgcat
     2041 cggccctctg cactgatacc gtacgaccga caaggtcatc gaggcctgct atgccgaact
     2101 cagtggctct cacgaatatg gccattgagg actcgagata cgcatcggag aacaagaact
     2161 gggattggcg cgctggagtg agcttcatac cctgtatagc gcagatatcg ccgtcttcca
     2221 gcgcccggcg gacgtgtgac cacggcatcg gaatcagctc aatctgaatc cccagctcga
     2281 ttcctatcgc gttcatcaag tccacattaa agccccgata agctccgcta tccgcaacat
     2341 actcgaatgg gggaaacgcc tcatcgccgc cgatcctgta cacatcgaag acactctgag
     2401 gtgcagcgct agaggatggt gcacccgagc aggttgcccc gagcgcaacc gccagaacac
     2461 acgccgctgc agcacggaac tgcacctggg atcgccgtcg tcgcgttgcg cacattgcca
     2521 tctcctccag cacccgcaat gaacattaag ttcaggcgcc atttgttttt ctgcgatttt
     2581 cggcaagtac tgatgttttt cctgcttgca tctgtacatg atgtaccgaa atcccatgct
     2641 cgacggcggc cgtcttgcga tcgggagtcg atctgtagcg catattgacc acttggtcaa
     2701 caccgtagta tcatgatcat gaacgcgttc acacgtcgca tgggaatgag gtgtgcatac
     2761 aatgcccctt atcgaagcca ggagcttgac ccgcgagtac tcccacggtc gcatgactgt
     2821 gcctgcccta agaggcgtgg acatcgaagt cgatatcggg gagttcaccg ccatcgccgg
     2881 gccatcgggt tcgggcaaga ccactctcct gaacctcata ggttgcctgg atcgcccgac
     2941 ccgcggcacg ctggtgatcg acggcgacga cgtcgcgcac tacacgccgt ccatgctagc
     3001 gagcctgcgg cgcgagaaga tcgggtttgt cttccagacc tataacctcg tgccggtgct
     3061 cacggcatac gagaacgtcg agctcccact ggttctcctt ggcaggcata cccacagcga
     3121 gatccgggac agggtcatgt cgatgctcgc cgaagtgggc ctggcggggc tggaaaaccg
     3181 gcggccgatg gacctctccg gcggccaaca gcagagagtc gccatagcaa gggcgctggt
     3241 caagcagccc aggctggttc tggccgacga gcctaccgcg aaccttgact ccgagaactc
     3301 cggtgccata ctggccttga tgaagaccat gaatgagacg cgaggcgcca cgttcctgtt
     3361 ctcgtcgcac gatccgctcg tgatcggcta tgccaggcgg ttggtgcaga tgcgcgacgg
     3421 caagatcgcg ctggacgaaa ggaggtagac agcatgctcc gcttctacct cggacttgcg
     3481 gccaagaaca tgtggcgccg caagagacga accatcctaa ccgcggccgc catcgccgta
     3541 gggatcatgt atttcgtcat gttcgactca ctgcttgccg gcgcagatac ggatgccgag
     3601 ctcaacacga tcgatttcga aacaggccat ctatgggtca gcgcttctga ggaaggcaat
     3661 gctgcactcg cagcactcga acgcccgacg cttgagaaga ccatcccgtc tgcccaggca
     3721 ctggcccaag ccatcgccga gctctccggc gttgccggcg tagcccctcg cctggtcttc
     3781 ccagcatcgc ttatcgcggg ccttgatgaa ctgcatgtct cagcaatagg agtcaatcct
     3841 gaggcagatt ctcaggtctt tcgggtatcg cgattcgtgt cctccggaag atggctcgaa
     3901 ccgggtgaag atggcatagt cctcggcaag cgcatcgcgg acctgctgca gctggaaccg
     3961 ggcgacacgg tcaccgtaa
//
LOCUS       DAOTAC010000242         3968 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_18403_length_3968_cov_4.607973, whole genome shotgun sequence.
ACCESSION   DAOTAC010000242 DAOTAC010000000
VERSION     DAOTAC010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3968
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18403_length_3968_cov_4.607973"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /locus_tag="PLM74_09600"
     CDS             complement(<1..861)
                     /locus_tag="PLM74_09600"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HON43365.1"
                     /translation="MAKGGKRTLVAGAVTLALAGAVTRGIGAVYHVVVVRVAGPEALG
                     LFQMAMPVYRLASGVASIGFHVAVVRLIADSLGRRRPDEARAYARSAVMAAMISGPMV
                     GLLLCFGAPCLATFLFKDIRLTLPIICLGLLLIPATISTILRGIVRGFGAAPSLAGAN
                     IAEAVLRVPGALVMLAVFLPFGMGSAASAMVGGMIVGELGSLAVLGFKTRSLMANRSL
                     GEPLLSRGLPPSRPKRDSGHIRALLRLGIPAMISGLLNSLISLVNAALVPRQLQSAGF
                     TQSGAAQAFGQ"
     gene            989..1402
                     /locus_tag="PLM74_09605"
     CDS             989..1402
                     /locus_tag="PLM74_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HON43366.1"
                     /translation="MRIDRDLTAASVVPLLLLLLEQEESYGYQIIRRIRSSSKQQWEW
                     SEGMLYPVLHRLEQRELIESYWRAAETGRQRKYYRLTKRGRAELQRQKQQWAVVQKTL
                     SSLGLPEVRGDMLDGRGKPDSGLEGGNSGEKPPCR"
     gene            1335..2675
                     /locus_tag="PLM74_09610"
     CDS             1335..2675
                     /locus_tag="PLM74_09610"
                     /inference="COORDINATES: protein motif:HMM:NF038403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HON43367.1"
                     /translation="MDVESQIAAWKAEIAAKSRLAVEDLGELEDHLRDQMEALQASGL
                     DKDEALLIAIRRMGNSTVLAGELARADLDRTWAQLPRAEEPGGGGNRREVGWVVLAAA
                     AAAALGKLPLLFGVQMAEGIDVYAKNMALFVIPVLIGGYYVVRRFHTSLLALLGAALA
                     LAAVAVNVYPFRGDGSTLLLTALHLPMLLWLLFGLAYTAGEWRNVRQVWDFIRFTGEF
                     VVYSVLIGLGGLAFMMLTTAFFSAIGLPIEDFLMEWVGYSGLLGIPVVAAYLVEKKRS
                     LIENIAPVLARIFIPMFLVMTVSFLVGALTRLHVLAQDRNLLIMVDVLLALVTGMVLY
                     DLSARAAEGPSTYMDLMSLLLMATALIIDILALGGILTRLSQFGLSPNRVAALGENVL
                     LLVNLAGLSYYYARFQRGKGSRREILSWQVRCMPGYLAWLAWVGFVLPPLYHFM"
     gene            2794..3225
                     /locus_tag="PLM74_09615"
     CDS             2794..3225
                     /locus_tag="PLM74_09615"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HON43368.1"
                     /translation="MRDKDIVERIKMAVVPVLTKRDQVVAAYLFGSTGTERATTMSDV
                     DIAVLTDGDISLLDELSLSADVAVALGREDVDLLALNSARNDLQHAVISEGELILDRD
                     PMKTSDFIEKVLSVHKDYGVFMRAFLSDLKSGLEEDYLGGR"
     gene            3215..3634
                     /locus_tag="PLM74_09620"
     CDS             3215..3634
                     /locus_tag="PLM74_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HON43369.1"
                     /translation="MVDESRILQKIQAITDSLSKLEELARMDRGTFLSDFRSVDSAKY
                     NLQTSIEAMIDICNHIISRRRLRIPATNVESFEIVSETGLLPADALPVYRSMARFRNR
                     IVHMYDEVDDSQIHEILQNRLGDIRAFVQSIVNTCWS"
BASE COUNT          781 a         1103 c         1246 g          838 t
ORIGIN      
        1 ctggccaaac gcctgagccg caccggattg tgtgaaaccg gcactctgaa gctgacgggg
       61 gaccagtgct gcgttgacga ggctgataag gctattgagc aggccggata tcattgccgg
      121 gatacccagt ctgagcaggg ctcttatgtg tcctgagtct cgtttcggcc tgcttggagg
      181 cagtcctctt gaaagcagtg gttcgcctag actcctgttg gccatcagtg atcgtgtctt
      241 gaacccaagg acggcgagag agccgagctc tccgacgatc atcccgccga ccatggccga
      301 ggccgccgac cccatgccga agggaaggaa tactgccagc ataaccagag cgcccggcac
      361 ccgcagcact gcctcggcaa tgttggcccc agccaaggag ggcgctgcgc caaatccccg
      421 aacgattcct ctgagaatgg ttgagatagt cgccgggatc agtagcagcc cgaggcagat
      481 gatggggagt gtaagcctga tgtccttgaa gaggaaggtt gcgaggcagg gcgccccgaa
      541 acacaggagc agacccacca tggggcctga aatcatcgca gccatcactg cagaccgcgc
      601 gtaggctcgg gcttcatcgg ggcgtctgcg accgaggcta tcggcgatta gcctcaccac
      661 tgcaacgtgg aaacctatcg aagcgacgcc agaggccagg cggtaaacgg gcatggccat
      721 ctggaacagt cccagagcct ctggcccggc cacccggaca acgacaacgt gatacaccgc
      781 accgattcct ctggtcaccg cgcccgccag agccagcgtt acggcgcctg caacgagagt
      841 tcgtttcccg cctttcgcca tctcgagacc caccatcccg cctattgatg gctattcggg
      901 ggcgtggtgg ggtagaccgg tggtggggcg gaccggcggt ggcggagatc ggttgtcata
      961 cctagcagcg ttaggtataa tatgaaccat gaggatcgat cgtgacttga cggcagcatc
     1021 ggtggtgccg ttgcttctcc tccttctgga acaggaggag agctacggct atcagatcat
     1081 acggcgcatc aggtcttcgt cgaagcagca gtgggagtgg tcggagggca tgttgtaccc
     1141 cgtgctccac cgactggagc aacgcgagtt gatcgagtcg tactggcggg cggccgagac
     1201 gggacgccag cgcaagtact atcgcctgac caagcgtggc cgagccgagc tgcagcggca
     1261 gaagcagcaa tgggcagtcg tgcagaagac gctgagcagt cttggcttgc cagaggtgag
     1321 aggagacatg ctcgatggac gtggaaagcc agatagcggc ctggaaggcg gaaatagcgg
     1381 cgaaaagccg ccttgccgtt gaggatctag gtgaactcga ggatcacctg cgcgaccaga
     1441 tggaggcgct gcaggcgtcc gggctagata aagatgaagc tcttctgatc gccattcggc
     1501 gcatgggcaa cagcactgtc ttggccggcg agttggcccg tgccgatctc gacagaacct
     1561 gggcacagct gccgcgggcg gaagaaccgg gcggaggggg caaccggcgc gaagtgggct
     1621 gggtggtcct ggctgctgca gcggctgccg ccctggggaa gctgcccctg ctcttcgggg
     1681 tgcagatggc ggaaggcatt gacgtctatg ccaagaacat ggcgctcttc gtgattccgg
     1741 tcttgatcgg cggttactac gttgttcggc gtttccacac gagccttctg gcgcttctcg
     1801 gggctgcgct cgcacttgcg gcggtggcgg tgaatgtcta cccgttccgt ggcgacggaa
     1861 gcactttgct gctcacagcc ctgcacttgc cgatgctgct ttggctgctc tttggcctgg
     1921 cgtacacagc cggggagtgg cgcaatgtac gccaggtgtg ggacttcatc cgcttcacgg
     1981 gagagttcgt ggtctactcc gtgctcatcg gcctgggcgg cttggcattc atgatgctta
     2041 ccacggcctt cttctcggcc ataggtctgc ccatagagga cttcctgatg gaatgggtgg
     2101 gctacagtgg gcttttgggt atccccgttg tggctgcata tctggtagag aagaagcgga
     2161 gtctgatcga gaacatcgcc ccagtcctag cccgcatctt cattccgatg tttctagtga
     2221 tgactgtctc ctttctagtt ggcgcgctga cccgtctaca tgtgttggcg caggaccgca
     2281 acttgctgat catggtcgac gtactgctgg cgctggtcac cgggatggtt ctctacgacc
     2341 tctcggcccg ggccgccgag ggtccgtcca catatatgga cctgatgagc ctgttgctga
     2401 tggcgaccgc tctgatcatc gatatcctgg ctttgggcgg catcctgacc cgtctcagcc
     2461 agttcggttt gagccccaac cgggtggccg ctctgggaga aaacgtcctg ctattggtca
     2521 acctcgccgg cctgtcatac tactacgcgc gtttccaaag gggtaagggt agccgccgcg
     2581 aaatcctgtc ctggcaggtg cggtgtatgc cggggtatct cgcctggctg gcctgggtcg
     2641 gcttcgtctt gccgcccctc taccacttca tgtaggcggc cgcctgctga ccttgcgcgt
     2701 gcatggtcag caggttcagg gactgataga atggaggcgc aggcgcaaag ggccagtgag
     2761 tggcaactgg tccggctgtc ggaggtggtc cgcatgcgcg ataaggacat tgtcgagcga
     2821 atcaagatgg ccgtagtgcc tgtcctcacg aagcgcgatc aggttgtggc tgcctatctc
     2881 ttcggttcta caggcacaga acgtgccact accatgagcg atgtcgacat tgccgttctc
     2941 actgacggcg acatctctct tttggatgag ctatcgctgt cggctgatgt ggcagtggcg
     3001 cttgggaggg aagatgtgga cctgctggcg ctcaactccg cccgcaatga tctgcagcat
     3061 gcggtcatat cagaaggcga actcatcctg gatcgcgatc ctatgaagac gtcagacttc
     3121 atagagaagg tcctatcggt ccacaaggac tatggcgtgt tcatgagggc ttttctgagc
     3181 gatctgaaat cgggattgga ggaggattac ctcggtggtc gatgagtcga gaattctgca
     3241 gaagatccag gctatcaccg attcgctctc aaagcttgaa gagctggcac gcatggacag
     3301 aggcacgttt ctcagtgact ttcggagcgt tgactcagcc aagtacaact tgcagactag
     3361 cattgaggcg atgattgaca tatgtaacca tatcatttcg cgcaggaggc tcaggattcc
     3421 ggcgacgaat gtagagtctt tcgagattgt cagtgagact gggctgttgc cggcagatgc
     3481 cttgccggtc tacagatcga tggctcgatt ccggaatcgc attgtccaca tgtacgatga
     3541 agtagatgat agccagattc atgagatact ccaaaaccgg ctgggcgata tcagagcctt
     3601 cgtgcagtcc attgtcaaca cgtgctggtc gtagaacatg ccgccctctc ctatgggcag
     3661 gtataaccga gtcttgaacc cgagaacggc ggaggagccg agctttccga cgatcatcgc
     3721 gccgaccgtg gccgtgcatc ccaaggtaga gaagttccga gcgctctggc ggcagcttag
     3781 tctgccagga tcgccgcctg gcacggcttt tcgagcatgc tgttcagatc ctacccgtca
     3841 aaaccggaca cattctagac agccctcgga gaaaaccggc tgttttcctt ctatgcatta
     3901 cccacatcca tggacatttc tgggtacggc gttctcctgt gtgggctcta acgagggttc
     3961 cggggcat
//
LOCUS       DAOTAC010000243         3953 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_18489_length_3953_cov_4.015393, whole genome shotgun sequence.
ACCESSION   DAOTAC010000243 DAOTAC010000000
VERSION     DAOTAC010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3953
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18489_length_3953_cov_4.015393"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1527
                     /locus_tag="PLM74_09625"
     CDS             <1..>1527
                     /locus_tag="PLM74_09625"
                     /inference="COORDINATES: protein motif:HMM:NF028259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43370.1"
                     /translation="VDKALVILGAEFRQFLNGSVGTGARRSKVRKFLKSWGGYVFLLA
                     IAIFIGRGVYKFAAGMNAALADYPDIARLIWVNLLSAASLTVFVMLFMTGVSVVYQSL
                     YEAGDVKFLLGTPAPVGAVVGAKLVMSLVTNLFAILPFLCPVWVGFGAASHAPASFYV
                     LVFLSQLLAAAMFTSFVALVVMAIMRYIPSPKMRQVILVGSLVIGFMIFAVTQALSAA
                     MSRNDGLSFEDIARIAQGVNLGRAAWLPHVWMLKTALLTMPGYGYSVSTSLLPLAAAA
                     VLISWAAIALSSRAFVGGWTHARETAPAAKGKASSAQHAGRTFINRLQGAGWAIFARD
                     LTMLFRQPVMWYGVLVSIVASAFFVFNISAAGNAAIIRDMIVVIFTMMAGVSTGQFAG
                     LAVSLDGEGLWLMKSAPIAPSTYYSAKLAFATAPGAVVLVLLFAGLSFVTSVPQHPLY
                     VSVPVGLAVLSVICALSVMSDAIKPNFSVRLSATGNQKQKDPGKALLVTFGSQIGSAA
                     L"
     assembly_gap    1528..1627
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1628..3144)
                     /locus_tag="PLM74_09630"
     CDS             complement(<1628..3144)
                     /locus_tag="PLM74_09630"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HON43371.1"
                     /translation="MPTDYSMAIKSALDFIENRVAEDIQPDQVAQVIGFSLSHSRAVF
                     AGVTDKTISQYIVGRKLSHAALALRSTDRPVSDIAMDYGFASHDSFTRAFRRAFGEPP
                     SRFRQQERSVGNMLITQGICGPAVRSMREKVILIDSANIVDPSSSVLYGVPKVSYFSA
                     ECECTPFPSSLKACLGFIGQPVTYHYLVAITGAAFRLLWNTEYWDGGNVGILIMRPNV
                     TEPLRRAMDAVGRDYTLLCKPEHAALLAGLQGESKSVKTGDKPAFVDLIRREIDAGRP
                     VISFGIIGPPEACVITGYRDGGETLLGWNFFQDMPEYASGIEKEPCGYFVRRDWYEHP
                     DTVAVLALGERKDEPSDEKALLIDTLTHALEIMETPRVHERAAGYAAYDAWADALLSE
                     SEFPAGAPLPLLMERLMCQIDGACMIGDRYQAHRFMQEQAALFPEAAVEMEKASQLFR
                     EEARLALQMGEAIGGMRMGEKQAQALAKRETREELVRLIRQAKELDMQAATHMKQA"
     gene            complement(3429..>3953)
                     /locus_tag="PLM74_09635"
     CDS             complement(3429..>3953)
                     /locus_tag="PLM74_09635"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019387610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HON43372.1"
                     /translation="TTEFMTFASMAEEFKALNCELIGLSIDSNFSHIAWLRTIKEKIK
                     FRDMENVEVKFPLIADLKMEVAKKYGMVQPAASDTQAVRAVFFIDPEGTIRALVYYPL
                     SNGRNFDEIKRLLIAMQTSDKFKVATPADWRPGEKVIVPPPGSCGQATERVAGAGKDY
                     ECQDWFLCFKNLPK"
BASE COUNT          718 a         1110 c         1134 g          891 t
ORIGIN      
        1 agtggacaaa gctcttgtca tacttggagc cgaattcagg cagttcctca acggatcagt
       61 tgggaccggt gctcgccgca gcaaggtgcg aaagttcctg aagagctggg gcggatacgt
      121 tttcctactg gccatagcca tcttcatagg caggggagtc tacaagttcg ccgccggcat
      181 gaacgcggcg ctggccgact accccgatat tgccaggctg atatgggtca acctgctgtc
      241 ggcggcctca ttgactgtgt tcgtaatgct gttcatgacc ggcgtatccg tggtatacca
      301 gtcgctgtac gaggcagggg atgtgaagtt cctactagga acgcccgcgc ccgtcggagc
      361 cgtggtaggt gccaagctcg tcatgtcgtt ggtgactaac ctgtttgcaa ttctaccgtt
      421 tctgtgcccc gtctgggttg gattcggggc cgcgtcgcac gctcctgcgt ctttctacgt
      481 tctcgtgttt ctatcacagc tgctagcggc ggcgatgttt acgtctttcg tggccctggt
      541 cgtgatggcc atcatgcggt acatcccgtc tcctaagatg cgtcaggtca ttctagttgg
      601 ctcactggtg ataggcttta tgatattcgc cgtgactcag gcgctgagcg ccgcgatgtc
      661 gcgcaacgac gggctaagct tcgaggacat cgctcgaata gcgcagggtg tcaacctggg
      721 cagggcggcg tggctgccgc acgtgtggat gctgaagaca gcgctactca ctatgcccgg
      781 ctacggctac tcggtgtcga cctcgctact cccactggct gccgctgccg tgctcattag
      841 ctgggctgcg atcgcgctgt cgtctcgcgc ctttgtgggg ggatggactc acgcaaggga
      901 gacagctccg gctgcgaaag gcaaggcgtc atctgcgcaa catgcaggcc ggactttcat
      961 caacaggctt cagggcgccg gatgggccat cttcgccaga gacctcacaa tgctcttccg
     1021 tcagcccgtg atgtggtacg gggtgcttgt atcgatagta gcgtcggctt tcttcgtgtt
     1081 caacatctcg gctgcgggaa atgcggcgat cattcgagac atgatcgtgg tcatcttcac
     1141 gatgatggct ggtgtgtcga cggggcaatt tgcaggcctt gcggtcagcc tggacggcga
     1201 gggactgtgg ctgatgaaat cggcgcctat cgcgccgtct acctattact cggcgaaact
     1261 cgctttcgca acagcgccgg gagctgtggt gcttgtgctt ctctttgcag ggctgtcgtt
     1321 cgtgacatca gtgccacagc accctctgta cgtgtcggtc ccggtcggct tggccgtgct
     1381 atcggtgata tgtgcattgt cggtgatgtc tgacgcaatc aagcccaact tcagcgtgcg
     1441 tctttcggca acaggaaacc agaagcagaa ggacccgggc aaagcccttc tggttacgtt
     1501 cgggagccag ataggcagtg cggcgctnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnngcc tgcttcatat gtgtggccgc ctgcatgtcg agctccttcg cttgccggat
     1681 gagccgaact agctcttctc tcgtctcgcg cttagcaagc gcctgcgcct gcttctcacc
     1741 cattctcatg ccgccgatgg cctcgcccat ctgcagggcc aggcgggctt cctcgcggaa
     1801 cagctgggat gccttttcca tctccaccgc agcctccggg aacagcgccg cttgctcctg
     1861 catgaagcga tgcgcctgat acctgtctcc tatcatgcac gcaccatcga tctggcacat
     1921 caggcgttcc atcagcaagg gaaggggcgc gcctgccggg aactccgatt cgctcagcag
     1981 ggcatcggcc caggcatcgt aagctgcgta accagctgct cgctcatgta cccttggggt
     2041 ttccatgatc tctagcgcgt gtgtcagggt atcgataagc aaggccttct cgtccgacgg
     2101 ctcatcttta cgttcgccca acgccaggac ggcaacggta tcgggatgct cgtaccaatc
     2161 tcggcgcacg aagtaaccgc aaggctcttt ctcaataccg gacgcgtact ctggcatgtc
     2221 ctgaaagaag ttccacccaa gtagcgtttc gccgccatcg cgatagcctg tgataacgca
     2281 tgcttcgggc gggccgatga tgccgaagct gataaccgga cgaccggcgt ctatctcgcg
     2341 cctgatcaga tccacaaaag ccggcttatc tccagttttc acagacttgc tctcgccctg
     2401 aagacctgca agtagagcag cgtgttcggg tttgcacagt aacgtgtagt ctctgcccac
     2461 cgcatccata gcgcgtcgca gcggttctgt gacgttcggc ctcataatca ggatgcccac
     2521 attgccgccg tcccaatact cagtgttcca gagcagcctg aaggctgccc cggtgatcgc
     2581 caccaggtaa tggtaggtta ctggctgacc tatgaaaccc aagcatgcct tcaatgaaga
     2641 gggaaatggt gtgcactcgc attctgcgct gaagtagctc actttgggaa caccgtacaa
     2701 cacagatgaa ctcggatcca ctatgttcgc gctatctatt aggatcacct tctctcgcat
     2761 tgatcgaaca gcgggcccgc aaatgccctg agtgatcaac atgttcccta ccgatcgttc
     2821 ctgctgccga aaacgcgaag gcggttcgcc gaaagctcgc ctgaaagccc tggtgaacga
     2881 atcatgtgag gcaaagccgt aatccatggc gatgtccgac acaggcctgt ccgtgcttct
     2941 cagcgcaagg gcggcgtggc tcagcttgcg accgacaatg tactgcgaga ttgtcttgtc
     3001 ggttaccccg gcgaagacgg cacgggagtg tgatagcgag aagccgatta cttgcgctac
     3061 ctgatctggc tgtatgtcct ctgccacacg attttcgatg aagtcaaggg ctgacttgat
     3121 cgccatcgag tagtctgtcg gcattgtgtc accgccttcg acgtagcttg ttctggtcgc
     3181 gcgaccatcc tgattatacg aggaccgggc agcgtccgct cgacaattct gatgaagcta
     3241 tcgttgggac ctgtgccgcg agagcgttcc gatgcgcttg gcagatggga atgtggatgg
     3301 gaatgtggat gggaatgtga aggaccgcgg gaaggcatga gccttgccgc ggtccacacg
     3361 agttacacac gcggtacacg cgcgccacac tgtgttgtgc tgctgctttt gctgcggtgt
     3421 gctacgttct acttaggcag attcttgaag cagaggaacc agtcctggca ctcgtaatcc
     3481 ttgcctgcgc ctgctaccct ctcggtggcc tgcccgcacg atcccggcgg cggaacgatg
     3541 accttctcgc ccgggcgcca gtcggcaggg gtggccacct tgaacttgtc ggaggtctgc
     3601 atggcgatga gcaggcgctt gatctcgtcg aagttgcgtc cattcgagag cgggtaatac
     3661 accagagcgc ggatggtccc ctcagggtcg atgaagaaga ctgcgcgaac ggcctgtgtg
     3721 tcgctggccg ctggctggac catgccgtac ttcttggcaa cctccatttt gaggtcggcg
     3781 atgagaggga acttgacctc tacgttttcc atgtcgcgga acttgatctt ctccttgatc
     3841 gtccgcagcc aggctatgtg gctgaagttg ctgtcgatgg agagaccgat cagctcgcag
     3901 ttgagcgctt tgaactcctc agccatcgat gcgaatgtca tgaactcggt ggt
//
LOCUS       DAOTAC010000244         3944 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_18528_length_3944_cov_4.120597, whole genome shotgun sequence.
ACCESSION   DAOTAC010000244 DAOTAC010000000
VERSION     DAOTAC010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3944
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18528_length_3944_cov_4.120597"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /gene="obgE"
                     /locus_tag="PLM74_09640"
     CDS             complement(<1..866)
                     /gene="obgE"
                     /locus_tag="PLM74_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014464676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HON43373.1"
                     /translation="MFVDYIEIRARGGDGGAGAVAFRREKYVPRGGPAGGDGGRGGSV
                     ILQADEALHTLVDLRYQKHLRAKNGQPGASSDKTGADGEDLTVRVPVGTIVRDIGTGE
                     VIADLLAHGQRVVLARGGAGGRGNARFATAVYRTPRFAEKGEAGEERVASMELRVLAD
                     VGLIGYPNAGKSTLLSKISSARPKIADYPFTTLSPVLGVVKAGDDSFVVADIPGLIEG
                     AASGVGLGHEFLRHIMRTRLLVHVVDVSEFSGRDPIRDYEAIRRELSLYEAGLETKPE
                     IVAANKMDMPGA"
     gene            complement(967..1686)
                     /locus_tag="PLM74_09645"
     CDS             complement(967..1686)
                     /locus_tag="PLM74_09645"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HON43374.1"
                     /translation="MSSIAFYAVVGVSVAVRTVVSRLPANRNRGVDIGDKLLCAILAL
                     TWLATGRDPLAATGISCTNLHLKVVRASAVAVPLLAGANMALQRRILIRHGDWVCRAG
                     VNPGGAFAAVSKLPFRTAVLAGLAYTSSGALLEELIFRWLLMAWVARVGGPLAGMAVQ
                     ATLFGLIHAAPMIACQAPIQVALYAFVMPLVCALVFGWLTAASGGVACAWIVHWALNY
                     IALVSALRSGRKEGPSAELRE"
     gene            complement(1683..3149)
                     /locus_tag="PLM74_09650"
     CDS             complement(1683..3149)
                     /locus_tag="PLM74_09650"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HON43375.1"
                     /translation="MDVHSLDPTTEYALAPSVKLVAANDGYIIMDPEDGVWVKLMRPA
                     GALLELVLDTSITEVIDTVGRKYGSDTLSELMDLLEELRAEGLLIRTRLVTRRTRDKS
                     SGTRYRRSHTGTQRAFLAVTDRCNLKCSTCYRSTGHDDAPTWQVERTISRAANLRVDE
                     LVITGGEPALRQDLPDLLYASQRAALRVTLATNGTLITPELAGAIANARVRVQVSLES
                     PSEREHDLIRGQGSFKAACRGLGLLAEAGVSDIEVVSTLLDPEVFSPDDMIAFAAQYR
                     ATFHASLFQEVGRGGCGLRRPAEDADALARSMLRYLMRRAREGSIPESAPFAEVVGIL
                     PRLGCGAGNSVVALSGAGDAYPCHLLMLPEFRADLSSLIDSPAEVRRQLKPKAWDPEK
                     DYASDMCNRYLQLSPEWRLPGVDSIPACEGCDIRYFCGGGCRAAAYAATGDVAGRDPS
                     CESYRALISSLLWAWDDRIPVIYNLYQACHRLGLDLSQ"
     gene            complement(3528..>3944)
                     /locus_tag="PLM74_09655"
     CDS             complement(3528..>3944)
                     /locus_tag="PLM74_09655"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HON43376.1"
                     /translation="ENGVVMLYLCPLLSGADSVDSFQHSGDSATAVASARARLEESID
                     KDVSEEGEAAAVRLTATETNVMRLLAEGKTNRAIAHEMFISINTVKTHVRAIFQKLGV
                     NTRGCAVTRAVVTGLIDWPGPEAPKITRLGNTRKMV"
BASE COUNT          812 a         1279 c         1086 g          767 t
ORIGIN      
        1 tgggctccgg gcatgtccat cttgttggcc gccacgattt cgggcttggt ttcgagcccc
       61 gcttcgtaga gcgacagttc tcgtcggatt gcttcgtagt cgcggatcgg atcgcgaccg
      121 gagaactcag acacgtccac aacgtgtacc aagaggcgag tgcgcatgat gtgacgcaga
      181 aactcgtgcc caagccctac gccgctggcg gctccctcga taagcccagg tatgtctgcc
      241 acgacgaagc tgtcatcacc agccttcact acacccagca ccgggctgag agtggtaaac
      301 gggtagtcgg cgatcttggg tctggcagag gagatcttcg atagcagcgt ggacttgcct
      361 gcattggggt agcctatgag gcccacatcg gccagcaccc tcagttccat agatgcaacg
      421 cgttcttctc cggcttcgcc cttctcggcg aacctcggag tacggtagac agcagtggcg
      481 aaccgggcgt taccgcgccc gcccgcgcct ccgcgggcca gcacaacacg ctgcccgtgg
      541 gccaacaaat cagcaatgac ttcacccgtg ccaatatccc gcacaatggt gccgacaggt
      601 acccgcacgg tcagatcctc gccgtcggct cccgtcttgt cgctgcttgc gccgggctgc
      661 ccgttcttgg cgcgcaggtg cttctgatat cgaagatcga caagagtatg caacgcctcg
      721 tctgcctgga gaatcacgct gccaccccgc ccgccgtcac caccggccgg gcccccgcga
      781 ggcacgtatt tctcgcgccg aaacgccacg gcgcctgctc ctccgtcacc acccctggcg
      841 cgaatctcga tatagtcaac aaacatccgc gtcgcgctcc tcccggcagc acaccagata
      901 catatcatct ccggcgacag tgtagtgtgc ccgtccgtcc atagcagtat atcagatgct
      961 gcccgattac tctcttaatt ctgcggaggg cccttccttc cttcccgagc ggagcgccga
     1021 cacgagagct atgtaattca atgcccaatg gactatccac gcgcacgcca cacctccgct
     1081 ggctgctgtc agccagccga agaccaaggc gcacacaagc ggcattacga acgcatacaa
     1141 tgccacctga atcggagctt gacaggcaat catgggcgct gcatgtatca gtccgaacaa
     1201 ggttgcctgg accgccatgc ctgcgagcgg accacccacg cgcgcaaccc atgccatcag
     1261 cagccagcgg aaaatgagct cctcaaggag agccccggac gacgtgtagg ccaaaccggc
     1321 cagcacggca gttctgaacg gcaatttcga aacagccgcg aaggcgcctc ccgggttgac
     1381 cccggcccga cacacccagt cgccatgacg aatcaagatg cgcctctgca gcgccatgtt
     1441 ggcccccgcc agcaaaggga ctgccactgc cgacgcgcgt accactttca ggtgcagatt
     1501 ggtacaggat atcccggtcg ccgcgagcgg gtcccgaccg gtggcaagcc aggttagtgc
     1561 caaaatggcg cagagcaact tgtcgccgat atcgacgcca cggtttctgt tcgccggcag
     1621 cctggacaca accgtcctga cggcgactga cacgccgact accgcgtaga atgcgatgga
     1681 actcattgag acagatccag acccagccgg tgacaagcct ggtagagatt gtagatcaca
     1741 ggtattctgt catcccatgc ccaaagcagg ctggaaatga gagctcggta cgactcgcag
     1801 ctgggatcgc gcccggccac atcgccagtt gcggcatatg ccgcagccct gcagcctccc
     1861 ccacagaagt agcgaatgtc acatccttca cacgcaggta tggagtcgac ccccggcaga
     1921 cgccattccg ggctaagctg cagatacctg ttgcacatgt ctgacgcgta atccttctcc
     1981 ggatcccagg ccttgggctt cagttgcctt cgtacttcgg caggcgagtc gatcagactg
     2041 gatagatcag ccctaaactc cggcagcatc agcaggtggc acggataggc gtcgcccgct
     2101 cccgacagcg ccactacgga attcccagct ccacacccaa gccgaggaag aatgccgacg
     2161 acttcggcaa acggagcgct ctccggaatc gatccttccc ttgcgcgccg catcaggtat
     2221 cgcagcatcg atcgggccag cgcatcggca tcttccgccg gtcgcctgag gccgcatccg
     2281 ccgcgaccta cctcctggaa tagactggcg tggaatgtgg cacggtactg tgctgcaaaa
     2341 gcgatcatgt cgtcgggtga gaacacctca ggatccagga gcgtagagac tacttcgata
     2401 tccgatactc ccgcttcggc cagaagccca aggccccggc atgcggcctt gaacgaaccc
     2461 tgccctctga tgaggtcgtg ttcgcgttca ctgggcgact ccaggctcac ctgcacgcgc
     2521 accctagcgt tcgcaatggc gcctgcaagt tcgggggtaa tgagggtgcc gttcgtcgcc
     2581 agcgtaacgc ggagagccgc tcgctgggat gcatagagca ggtccggcag gtcttgacga
     2641 agcgcaggct cgccgccggt aatcacaagc tcatctacgc gcaggttggc agccctcgat
     2701 atcgtgcgct caacctgcca agtcggcgca tcgtcatggc ccgtgctacg ataacacgtg
     2761 gagcacttca ggttgcacct gtcggtgacc gccaggaaag ccctttgtgt gccggtgtga
     2821 gagcgccgat atctagtccc tgagctcttg tctcgcgttc ttcgcgtcac aagcctggtg
     2881 cggatcaaca aaccttcggc tcgcagttcc tcaagcagat ccatgagctc agatagggtg
     2941 tcgctgccat atttcctgcc gacggtgtcg atcacctctg taatactggt atccaacacc
     3001 agttcgagca gtgctcccgc aggtcgcatc agtttgaccc acacaccgtc ttccgggtcc
     3061 attatgatgt agccatcgtt ggcagcgact aatttcacgc tgggagcaag tgcgtactca
     3121 gtagttgggt ctagcgagtg aacgtccatg ccaagaacag agcaaccgac gtctgcatct
     3181 gctctgcaga ccggtgcccc ctcctttctt gcttgggtgg gtaattgcgc agatagggag
     3241 gaaccaacca ggcccaaggt tggctccggt acgcctgagt acgagatccg gtacgcagcg
     3301 ccgctgtaag gattacggag actgcacgct gaagagaccg gctatccgat ggtgatgaga
     3361 tagccccaca ggatgaaatt gacacagaag ctgttcaccg acacgttcat ccggcgcttt
     3421 cgccctgctg tgacttcaaa cacggttgcc acctcctttt tcgtgtaggg aacgatccga
     3481 gaccaaacgg ccgcaccgcg accgcgcttg catccacagc ataacatcta caccatttta
     3541 cgggtattac ccaaacgggt gatttttggc gcctcgggtc ccggccagtc tatgagccct
     3601 gtgacgacag cccgggtgac agcgcagcct ctagtattga cccccagctt ttggaatatg
     3661 gctcttacat gcgttttcac cgtattgatc gagatgaaca tctcgtgtgc gatcgcgcgg
     3721 ttggttttgc cttcggccag gagcctcatc acgttagtct ctgtggcagt gagcctgacg
     3781 gctgcagcct caccctcctc cgatacatcc ttgtctatgc tctcttcaag cctggcccgg
     3841 gcagatgcga cggccgtcgc gctgtctccg ctgtgctgaa acgaatccac actgtcagcg
     3901 ccgctcaaga ggggacacaa gtacagcatt acaacaccgt tctc
//
LOCUS       DAOTAC010000245         3910 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_18736_length_3910_cov_3.757717, whole genome shotgun sequence.
ACCESSION   DAOTAC010000245 DAOTAC010000000
VERSION     DAOTAC010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3910
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18736_length_3910_cov_3.757717"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..989
                     /gene="priA"
                     /locus_tag="PLM74_09660"
     CDS             <1..989
                     /gene="priA"
                     /locus_tag="PLM74_09660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HON43377.1"
                     /translation="RGFSTFVLCRECGHVMTCPHCDVSLTLHGSGSRRSELVCHYCNH
                     TERAPDVCPSCGGTHIRYFGAGTERIEDEVKKSFPEARVARMDVDTTRRKGAHRAILG
                     AFRAGHTDILVGTQMIAKGLDFPNVTLVGVVSADTALNLPDFRAAERTYQLISQVAGR
                     SGRGDAPGTVVVQTYSPEHYSIVAAAAHDYRAFYEAEIASRREAGYPPARRMARILVR
                     GIDESATARCAGMIASACRELDCVSDGTVEVLGPAPAPIPRIAGKYRWHLLLLSGDFQ
                     ALRGAAKAGCAAREAGRGAGGGAGGVGGVRGIGGGPAAGITVTLDIEPSAVL"
     gene            1055..1549
                     /gene="def"
                     /locus_tag="PLM74_09665"
     CDS             1055..1549
                     /gene="def"
                     /locus_tag="PLM74_09665"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019554153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HON43378.1"
                     /translation="MLILEIRKDGDPVLRQKAAPVAVIDDEMRQLAADMLETMYNAPG
                     VGLAAPQVGVAVRLIVVDDGSGPHTLFNPRVVSRRGEVCEEEGCLSVPGMIGKVRRAA
                     QVEVEAYDRSGRRILVRGDDLMSIILQHETDHLDGVLFTDKAESLRRAEASSENRETE
                     AEGE"
     gene            1559..2527
                     /gene="fmt"
                     /locus_tag="PLM74_09670"
     CDS             1559..2527
                     /gene="fmt"
                     /locus_tag="PLM74_09670"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HON43379.1"
                     /translation="MVFMGTPAFAVPTLEALADAGHDILCVVTQPDRPAGRGHKLAPP
                     PVKVAAQARGLRVLQPERVRAAVFVQAMRDLAPDVAVVAAFGQKITAELLAVPAHGFV
                     NVHPSLLPRYRGAAPVERCLLNGDSVTGVTIMYMDEGWDTGDIGICREFAVPRDARAN
                     AGWLEESLARVGAELMVQFLRLLEEGRAPRTAQDHSMASYAPKISRDELEIDWRRRAS
                     QLFDTVRGLAPDPGARTTLDGQQLRVLCLRELGKGEAEGDSEVGPAVKPGTIMLGPSD
                     AVIVACGENGRERVTLEEVQPSGKRCMTGAEFARGRRICTGARLGS"
     gene            2639..3340
                     /locus_tag="PLM74_09675"
     CDS             2639..3340
                     /locus_tag="PLM74_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005337165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HON43380.1"
                     /translation="MMYPFFYDPTYILVLPAFIFALYAQTRVQSAFTRGMNIRSRAGV
                     TGAQVANSLLADRGVDVSVEQIGRPMGDHFDPRSGVVRLSPQVYGGTSIAALAVAAHE
                     TGHAMQHSEGYAPLSVREAILPVASFGSTMALPLFILGMILGSPQISRFLMTLGIWLF
                     AGAVAFQIVTLPVEYNASRRALAMLTEAGYVTDAETPQVKNVLNAAALTYVAAAAVAL
                     TQLLRLLVLRGSRRD"
     gene            3333..>3910
                     /locus_tag="PLM74_09680"
     CDS             3333..>3910
                     /locus_tag="PLM74_09680"
                     /inference="COORDINATES: protein motif:HMM:NF013216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HON43381.1"
                     /translation="MTKRPDFRKASASGRDLAVEILYTCDQDGAFAHAAWSRALSGRN
                     LDARERRLGMELAFGSIRRKKTLDWCIARAAGRSMQDVDPLAHSLLRISTYQLLFMSR
                     IPAHAAVSEAVESARRLGHPGMAGFVNAVLRRIASPRFDPGFPDLADDPVKSLAVTLS
                     YPEWLVESWIGEFGPETAKRMLEAGNQAPTVV"
BASE COUNT          697 a         1082 c         1358 g          773 t
ORIGIN      
        1 gacgcgggtt ttctacgttt gtcctgtgca gagagtgcgg ccacgtgatg acctgcccgc
       61 actgcgatgt ctcgctgacg ctccacggtt cggggtcgcg ccggtctgag ctagtatgtc
      121 actactgcaa ccatactgaa cgcgcccccg atgtctgtcc gtcctgcggc ggcacgcata
      181 tccggtactt cggcgccggc accgagcgga tcgaggacga ggtcaaaaag tccttccccg
      241 aagcgcgagt cgcgcgcatg gatgtcgaca ccacgcggcg caaaggggcg caccgggcga
      301 tactcggtgc attcagggcc gggcacaccg atattcttgt gggaacacag atgattgcca
      361 agggtctgga ttttcccaat gtcacgctgg tgggagtggt gtcggccgac actgccctga
      421 acctgcctga tttcagggcg gcagagcgga cttaccagct catttcgcag gttgccggca
      481 ggtcgggacg tggggatgcc cccggaactg tggtcgtgca aacttacagc ccggagcatt
      541 acagcatcgt ggcggcagca gcccacgact atcgggcatt ctacgaggct gagattgcat
      601 ctagacgcga ggcggggtat ccaccggcgc gtcggatggc ccggattctt gtgagaggaa
      661 tcgacgagtc ggccaccgcg cgatgcgcgg gcatgattgc tagtgcatgc cgtgagcttg
      721 actgtgtgtc agacggcaca gtcgaagtgc ttggcccggc gccggcgccc attccccgaa
      781 ttgcggggaa gtaccggtgg catctgctgc tgctatcagg tgattttcag gcgttgcgcg
      841 gggctgcgaa ggctggatgc gctgcgcgtg aggcgggccg cggcgcaggg ggcggcgcag
      901 ggggcgtcgg cggcgttcgc ggcatcggcg gcggcccggc ggcagggatt acggtgaccc
      961 ttgacattga gccgtcggcg gttctgtgat tgggcaatgc gcctatcgat gcgctataat
     1021 gtagcaaggc aacataccat tttaggaggc aactctgttg attctcgaga tacgcaagga
     1081 cggcgatccg gtgcttcgcc aaaaggccgc gccggtggct gtgatcgatg atgaaatgcg
     1141 ccagctggct gccgatatgc tggagactat gtacaacgct ccgggggtag gactggctgc
     1201 gccgcaggtc ggcgtggcgg ttcgactcat agtcgtggac gatggttcgg ggccccacac
     1261 gctcttcaac ccccgggtgg tgtcgcgccg cggggaagta tgtgaggagg aaggatgcct
     1321 gtcggttccg gggatgatcg gaaaggtaag gcgggcggct caagtggaag tcgaggcgta
     1381 cgacaggtca ggccggagaa tcctggtccg cggcgacgat ctgatgtcga taatccttca
     1441 gcatgaaacc gaccatcttg acggcgtgct cttcactgac aaggcagaaa gcctgaggcg
     1501 cgccgaggcg agtagcgaga accgcgagac ggaagcggag ggcgagtaac cgttgcgagt
     1561 ggtgttcatg ggtactcccg cgtttgccgt tcctacgctg gaagctctgg cagacgcggg
     1621 gcatgatatc ctatgtgtgg tcacacagcc cgacaggcct gccgggcgcg gccacaaact
     1681 tgcgcctcct cctgtgaagg ttgcagccca ggccagaggg ctgagggtac tgcagccgga
     1741 gagggtgcgg gcggcggttt tcgtgcaggc catgcgcgat ctggcgccag acgtggctgt
     1801 ggtggcggcc ttcggccaga agatcacggc agagcttctg gcagtgcccg ctcacgggtt
     1861 cgtgaatgtc catccgtcgc tgctgccgcg atacaggggc gctgctcccg tagagcgctg
     1921 cctgctcaat ggagactccg taaccggagt aaccatcatg tacatggatg agggatggga
     1981 cacgggtgac atcggcatct gccgggagtt cgcggtgcct cgcgacgctc gcgccaacgc
     2041 aggatggctg gaggagtcgc tcgcgcgggt gggtgcggaa ctgatggtgc agtttcttcg
     2101 tttgcttgaa gaggggcgag ctccacgcac tgcacaggac cactccatgg ccagctacgc
     2161 accgaagatc tctcgagacg aattggagat agactggcgc cgcagggcat cgcagctgtt
     2221 cgacacagtg cgagggctgg cgcccgaccc gggagcgcgc actacgcttg acgggcaaca
     2281 gctgcgagtg ttatgcctgc gtgagctggg caagggagag gctgagggcg actcggaggt
     2341 ggggccggca gtcaaaccag gaacgataat gctggggccg agcgatgctg tgatagtggc
     2401 gtgcggcgag aacggccggg aacgggttac gctcgaagaa gtgcaaccgt cagggaagcg
     2461 gtgcatgacc ggggcggagt tcgcccgggg gcggcgcatc tgcacaggcg cccgattggg
     2521 cagctagcgt agcgctgtgg gcagttgcgg cgtttgcacc ttcagctgaa gccttgaggc
     2581 gtgtggagtg aactgtgcag cttatcgatt tcgcagatga tgttgggggg tgacgcggat
     2641 gatgtaccca tttttctatg atcccacgta cattctggtg ttgccggcgt tcatctttgc
     2701 actgtacgcg cagacgcggg tgcagtctgc cttcacaaga ggcatgaaca tccgctcccg
     2761 cgcgggagtt accggcgccc aggtagccaa cagccttctg gccgatagag gagtcgatgt
     2821 gtctgttgag cagatcggcc ggcccatggg agaccatttc gacccgcgca gcggcgtggt
     2881 gcgtctatcg cctcaggttt atggaggaac gtccatagcc gcgttggccg tggccgcgca
     2941 cgagacggga catgccatgc agcacagcga gggctatgcc ccgctttcgg ttagggaagc
     3001 cattctgccc gtggcaagct ttggctcgac tatggccctg cccctgttca tactcggcat
     3061 gattctgggc agcccccaga taagccggtt tttgatgacc ctggggatat ggctgtttgc
     3121 tggggcagtg gcatttcaga tcgtaacgtt gccggtggag tacaacgcca gccggcgggc
     3181 tctggctatg cttacggagg ccggttatgt caccgatgct gaaacgccgc aagtgaagaa
     3241 tgtactgaat gctgctgcat tgacgtatgt ggctgctgcg gccgttgcac tgactcagct
     3301 actgcgactc ctagtgctca gaggcagccg gcgtgactaa gcgtcccgat ttccgcaaag
     3361 cctcggcgag cggcagggac cttgcagtgg agatcttgta cacctgcgac caggacggtg
     3421 cgttcgcgca cgctgcctgg tcgcgcgctt tgtcaggccg taatcttgat gcgcgcgagc
     3481 gccgccttgg aatggaattg gccttcggct caatacgccg gaagaaaact ctggactggt
     3541 gtatagctag ggcggccggg cgctcgatgc aagatgtcga tccgctggcg cactcgttgc
     3601 tacgcattag cacctaccag ctcctcttca tgtcgcgtat cccggcgcac gccgcggtat
     3661 cggaggctgt ggagtccgct cggcgactgg ggcatccggg tatggccgga ttcgtcaatg
     3721 cggtgctgcg tcgcatcgct tcgcccaggt tcgatccggg ttttcccgat ctggcagatg
     3781 atcctgtgaa atcgctcgct gtcaccctgt cctatcctga gtggcttgta gagtcatgga
     3841 tcggcgagtt cgggccggag actgccaagc gtatgctcga agcgggcaac caggctccga
     3901 cggtggtgtt
//
LOCUS       DAOTAC010000246         3909 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_18741_length_3909_cov_3.392839, whole genome shotgun sequence.
ACCESSION   DAOTAC010000246 DAOTAC010000000
VERSION     DAOTAC010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18741_length_3909_cov_3.392839"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            297..1643
                     /gene="spoIVB"
                     /locus_tag="PLM74_09685"
     CDS             297..1643
                     /gene="spoIVB"
                     /locus_tag="PLM74_09685"
                     /EC_number="3.4.21.116"
                     /inference="COORDINATES: protein motif:HMM:TIGR02860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase"
                     /protein_id="HON43382.1"
                     /translation="MRAHSRRLAIILCVLLSLLTLAAGGVLRAIGLRPAYLRVVEGQE
                     FDLNSGFNLSGAFVADPPDSLLIDQAAGGSISVTPTKPGRARVEFRLFNAVAVRRFVV
                     DVVPELRVALGGHSIGILLASHGVRVIGLYPVIGTDGGRYMPARDAGLAVGDAILTVD
                     GREVSDPVELETAVNQVSSVRGAVTLGILHEGKQREVVVEAVPTDSGFRIGVYVRDNA
                     AGVGTLTFWHPGSLRFGALGHLVTDSQTGKEIPITDGRIVAARVAGIDAGAVGRPGEK
                     IGTFAGTRDNVGVIEINSSIGIYGRLTTPLTNSAFPEPIPVALASSVHEGPAEILTVI
                     SGSTIERFRVDIVKVARQTRPSGKGMVIRVVDPRLIERTGGIVQGMSGSPIVQDGKLI
                     GAVTHVFVSDPTRGYAVFAEYMLDAAGILQEQPEPLSWAEPRQKAHTRADLAHHIM"
     gene            1711..1974
                     /locus_tag="PLM74_09690"
     CDS             1711..1974
                     /locus_tag="PLM74_09690"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HON43383.1"
                     /translation="MPETKPETDSSALLALEKLSPREREIIKMVALGLSNQSIGERLF
                     ISDKTVKNYVTSIRRKLGVENRIQIALHAIRCGLVDPGISQQQ"
     gene            2036..2821
                     /gene="spo0A"
                     /locus_tag="PLM74_09695"
     CDS             2036..2821
                     /gene="spo0A"
                     /locus_tag="PLM74_09695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HON43384.1"
                     /translation="MLESRIRVVIVDDNMEFTAVVEEFLSRQPDMVVMGTAYNGKAAI
                     ELIEEVHPDVVVLDVVMPHLDGLGVIEYIAAAGFARRPRIVVVSAIGLETTAASILGA
                     GADYYLAKPFDLSILAERIRRLAAIPPDIRSSIRPSMPTRTTSLEFEASRALREIGIP
                     SNLKGYVYLRDAIIFALSEGDMLSSITHVVYPAIARRHNTTASRVERSMRHAIETAWM
                     RGDINTLTDVFGHTVDAEKGRPTNSAFIARIADKIRLDRAATR"
     gene            2859..>3909
                     /gene="steA"
                     /locus_tag="PLM74_09700"
     CDS             2859..>3909
                     /gene="steA"
                     /locus_tag="PLM74_09700"
                     /inference="COORDINATES: protein motif:HMM:NF040608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cytokinetic ring protein SteA"
                     /protein_id="HON43385.1"
                     /translation="MDASDDSGPDHAGLSEGQTEATTAATARVGRKTKHLLERLAPGE
                     IAVISHADIDEVSARALAAKRPAVIINAEPCITGRYRCPGPAILMDAGIPVLDCVGDQ
                     VMESIADGAEVQVRAGRVYAGGGMVGSGRCFTREHLADAYAAAEENRGAELERFVTNT
                     LHYALQEKDMILGELDVPTIKTRIRGRHCVIAVRGSNYLEDLAAVSSYIREVRPALIG
                     VDGGADALLDAGLVPDMIVGDMDSVTDKALLSGAELVAHAYPDGRSPASDRLRSLGLA
                     HSVFRCPGTSEDIAMLLAHDKGASLIVMLGSHSNMLDFLEKGRPGMGSTFLVRLKVGS
                     VLVDARGVSQLYRGRL"
BASE COUNT          773 a         1109 c         1242 g          785 t
ORIGIN      
        1 ttcagccgtg ggtccgctct cttggcgccg tggttgaccg cggccctttc atcgctggtt
       61 tagacgtgat tatggtgtct ttacgatagc acacactacg aggaaaagca atcggccccc
      121 gctgcggcgg gtttcaaggt agcatagcgc agcgtgaggc gctcgtgaat atgcccgaat
      181 ccacccggac aggttaattc cgagcgggcg gattcgggcg cgctgtgctg tgcagcggag
      241 gccctgcgcg attgcccgca gctggcgccg tttgggcgct gtaatgcggg ggcagtatgc
      301 gggcacattc gcgacgtctg gcgattatcc tatgtgttct cctgtcgctc ttgactctgg
      361 ccgctggagg cgtgcttcgc gccatcggcc ttcgaccggc ctatctacgc gtggttgagg
      421 ggcaagagtt cgatctcaat tccggtttca acctgtccgg cgcgttcgtc gctgatcctc
      481 cggattcact cttgatcgac caggccgccg gaggcagcat ctcggtcaca ccaaccaagc
      541 ctgggcgtgc ccgcgtggaa tttcgtctgt tcaacgccgt agcggtgcgg cgctttgtcg
      601 tggacgtcgt gcccgaactc agggtggcgc tgggcggaca ttccatcggc atactcctgg
      661 ctagccatgg ggttcgtgtg atagggctgt atcccgtcat cggcacagac ggcggcagat
      721 acatgcccgc acgcgacgca ggtctcgcgg ttggagacgc aattctgaca gttgacgggc
      781 gtgaggtttc tgaccctgtc gagcttgaga cggcagtaaa ccaggtctcc tcggtgcgcg
      841 gagcggtaac cctcggcatt cttcacgagg ggaaacagag ggaggtcgtg gttgaggctg
      901 ttccaactga ctcgggattc aggatcggcg tctatgttcg cgataacgct gccggcgtag
      961 gcacgcttac cttctggcac cccggcagcc ttcggttcgg ggcgctcggg catctggtaa
     1021 cggacagcca aacaggtaag gagatcccca tcaccgatgg gcggatagtg gctgcccgtg
     1081 tggcgggaat tgatgcgggc gcggtggggc gtccgggcga gaagataggc acgttcgccg
     1141 gaacccgcga caatgtcggc gtgatcgaaa tcaactcgtc gatcgggatt tacggcaggt
     1201 tgactacgcc gcttaccaat tccgcattcc ccgagccgat tccggttgcc ctcgcctcct
     1261 cggtccacga ggggccggca gagatcctca cggtaatctc cggatcgacg atagaacgct
     1321 tccgagtaga cattgtcaag gtcgctaggc agacgcgtcc gtcgggcaaa gggatggtaa
     1381 tccgagtcgt cgacccccgg ctgattgagc ggaccggcgg gattgtgcag ggcatgagtg
     1441 gatctcccat agtccaggac ggtaagctca tcggcgccgt cactcacgtc ttcgtttcgg
     1501 acccgacaag aggatacgct gtgtttgccg agtatatgtt ggatgccgca ggcatcttgc
     1561 aggagcagcc ggaaccgctg tcatgggccg aaccacgtca aaaagcccat acacgggctg
     1621 atttggcaca tcatattatg tagcgccaga gggagaagca agcctgcttc agctgtgtat
     1681 acgccctgac ggggttgaga ggtgaggcct gtgccggaga ctaagccgga aacggactcg
     1741 tccgcattgc ttgcgctgga gaaactctct ccgcgcgagc gcgagatcat aaaaatggta
     1801 gccctcgggt tgagcaatca gagcatcggg gagcgtctgt tcatttcgga caaaaccgtg
     1861 aaaaactacg ttacatcgat acgccgtaag ttgggcgtcg agaaccggat tcagatcgcg
     1921 cttcacgcga tccgctgcgg gctggttgac ccgggtatca gtcagcagca atagagggaa
     1981 acagtgccac cgcgtagaaa gaataatggc aagttcttcg cggaggcttt gcggaatgct
     2041 tgagagtcgc atacgcgtag tgatcgtgga cgacaacatg gagttcacgg cggtggttga
     2101 ggagttcctt tcgcgtcagc ccgacatggt tgtgatgggc acggcgtaca acggtaaggc
     2161 cgccatagag ctgattgaag aggttcaccc ggatgtcgtg gtgctcgacg tagtaatgcc
     2221 gcacctcgac ggactgggag tcattgagta cattgccgcc gcgggctttg cgcgccgccc
     2281 gcggattgtg gtcgtatcgg cgataggtct cgaaaccacc gcggcaagca ttctgggtgc
     2341 cggggccgac tattacctag cgaagccttt cgacttgagc atattagccg aaagaatccg
     2401 gcggctcgcg gcgatcccgc cggatattcg gtcgtccata cggccgagta tgcccacgcg
     2461 caccacttca cttgagttcg aagcaagcag ggcactgcgc gaaatcggta ttccctccaa
     2521 cctcaagggc tatgtctacc tgcgcgatgc tatcatcttt gctctcagcg aaggagacat
     2581 gctctccagc atcacgcatg tggtgtaccc tgccatcgcc cgcaggcaca atacaacggc
     2641 atcccgggtt gaacgatcca tgcggcacgc cattgaaact gcctggatgc ggggtgatat
     2701 caacactctg actgacgttt tcggtcatac agtagacgcc gagaaagggc gccccaccaa
     2761 ctctgccttc attgcccgaa tcgcggacaa gatccggctc gacagggcgg ccaccagata
     2821 gtgcgaatgc acgacggatc tgacggaatc ctagtagcat ggatgcttca gacgatagcg
     2881 gacctgacca tgcaggcctg agcgaagggc agaccgaggc gacgaccgca gcgacggccc
     2941 gcgtcggccg caaaaccaag cacctcttgg aaaggctagc gcccggcgag attgccgtga
     3001 tatcccacgc cgacatcgac gaggtttcgg cgcgtgctct tgcggccaag cgaccggcag
     3061 tgataatcaa cgctgagccg tgcataactg gccggtaccg atgcccggga ccggccattc
     3121 tcatggatgc gggcatacct gtgctggact gtgttggcga tcaggttatg gaatcgattg
     3181 ccgacggagc agaggtccag gtgcgggcag gccgtgtgta tgcaggcggc gggatggtgg
     3241 gaagcggacg atgctttacg cgcgagcatc tggcagacgc ctacgcagcc gcggaggaga
     3301 atcgcggcgc cgagcttgag cggtttgtca ccaacaccct gcactatgcg ttgcaggaaa
     3361 aggacatgat cctgggcgag ttggacgtgc ccaccatcaa gacccgtatc cgtggacgcc
     3421 attgcgtcat agcggtcagg ggttcaaact acctggaaga cttggccgct gtaagctcct
     3481 acattagaga agtcaggccc gcgctgatcg gtgtggacgg tggcgcggac gccctcctcg
     3541 acgccggcct tgttcccgac atgatagtcg gagacatgga ctcggtcacc gacaaggcgc
     3601 tgctttcagg ggccgaactc gtcgcccacg cctatcctga cggcaggtct cccgcctccg
     3661 acaggcttcg aagcctgggc cttgctcatt cagtcttccg ctgccctggc accagcgagg
     3721 acattgccat gcttctggct cacgacaagg gagcgtcctt aatcgtgatg ctcggttctc
     3781 attcgaacat gctggacttt ctggagaaag gccggccggg catgggcagt acgtttctgg
     3841 tcaggctcaa ggtcggttca gtgctggtgg acgccagagg agtgagccag ctgtaccggg
     3901 gaaggctac
//
LOCUS       DAOTAC010000247         3905 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_18762_length_3905_cov_4.622597, whole genome shotgun sequence.
ACCESSION   DAOTAC010000247 DAOTAC010000000
VERSION     DAOTAC010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3905
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18762_length_3905_cov_4.622597"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="PLM74_09705"
     CDS             <1..820
                     /locus_tag="PLM74_09705"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HON43386.1"
                     /translation="DRATAMGCCESLAQGVMGLAGPVIGAVLVTRFGGVSTQGIRPLF
                     LIASALSLVSLLLISTQLSQTEYVAAEKASLRAGLRPLLLLGERPDLWRFVAVSCLAN
                     LPTGMVLPFTQVFAREMKMADQYVLGAMVTAFAVVGLLLGVPLGRLADRFGRKRILYA
                     LAPVFWASNLLLVWSPSPVLLVFAGMLQGTVSMTLVVTQAMAFELVPSSRMSDWLAVL
                     RFFRMLVGAALAFVSGIIWDHFGPEYVFLLAVGIDAAIRIPLLAGLPETLDAPV"
     gene            complement(887..1192)
                     /locus_tag="PLM74_09710"
     CDS             complement(887..1192)
                     /locus_tag="PLM74_09710"
                     /inference="COORDINATES: protein motif:HMM:NF039800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6504 family protein"
                     /protein_id="HON43387.1"
                     /translation="MLTNGVMLVPYFIGKPAKVECSPGGTAPTALAWNDTTVQVVEIL
                     SEWQDFGFGGTHPSARTWRTRRHRNYYRVLCDNGHTYEIYLDRGSERRQWHVYRQVD"
     gene            complement(1314..2054)
                     /locus_tag="PLM74_09715"
     CDS             complement(1314..2054)
                     /locus_tag="PLM74_09715"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HON43388.1"
                     /translation="MCPEVLRLDENSPIPLYEQLASQLRDKIRAGELEPGEPIPTEAE
                     LSAMYGVSRTTARQAVLTLTRDGLVHRKRGKGSFVSQPKIVQQLVTLRSFSDEIRILG
                     FKPGARLIGKEWVPADEDVAQALQVKAGDPVLRIVRLRLAGEKELSINASHFARHHGL
                     ILENFDLGAPSLYCLVEAHVGPVTRATEVITAVTATEEQARHLIVPPGAPLLRLGRTT
                     YISGGYPVEFVRADFRPDRYTFFVELKA"
     gene            2342..3181
                     /locus_tag="PLM74_09720"
     CDS             2342..3181
                     /locus_tag="PLM74_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HON43389.1"
                     /translation="MHEPMERFRSVFSEPKPVIGMVHLPPLPGAPRYNGAQMADIAEY
                     AISEARKLEETGYSGLIVENFGDAMFYKRARPETIAAMTYVAKALADAVSLPMGICVL
                     QSDAIAAISIAHVVGARFVRVPYYTETYVVDAGLMESCAADALRFRKLLGSDALIFAD
                     VHIKHGYPLSQRPIQESAEDAVHRGLADVVIVTGKKTGGPTNVEDVQKVKTQIPQYPV
                     LAGSGISTADAHILLGIADGAIVGTNLKINGDTESPIDLCRAKELMTVIQGIRQQEGD
                     DQR"
     gene            3178..>3905
                     /locus_tag="PLM74_09725"
     CDS             3178..>3905
                     /locus_tag="PLM74_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HON43390.1"
                     /translation="MKSRLTAFNEMFGHKAIIGMVHLPPLPGSPLYDDKGISSTIEFA
                     MDEARKLQDGGVDAIEIENYLDASYFPDAVGPELVSAMAIIAHEVRKVTDIPMGICIL
                     ADPTAGLAVAHAIRAQFVRATFFTEASVDVSGLVLARPHQLLRYRKFLDPSVKIFADV
                     HIKHSAPLAPRPIEESAYDAAYFQADAVIVSGKHTGFPTRMDDVRAVKRVLPEVPVLI
                     GSGLRQSTAAEFFEVADGAIVGSSL"
BASE COUNT          783 a         1099 c         1135 g          888 t
ORIGIN      
        1 agacagggca actgcgatgg gttgttgtga gagccttgcg cagggagtga tggggcttgc
       61 cgggccggta atcggagcag tacttgtcac ccggtttggc ggagtcagca ctcaggggat
      121 caggccgctg ttcctgatcg cttcggctct ttccctcgtg tcgctcctcc tcatatccac
      181 ccagctgtcg cagaccgaat acgttgcggc ggagaaggca agcctccggg ccggcctcag
      241 gccgcttcta ttgctgggcg agcgccctga tctgtggcgc tttgtagccg tgtcctgcct
      301 agccaacctg cccacaggca tggtgcttcc gtttacccag gtgtttgcac gcgagatgaa
      361 gatggcagac cagtacgtgc tgggtgcaat ggtaaccgcc tttgcagtgg taggtctgtt
      421 gcttggcgtt cccctgggca ggttggccga tagatttggg cgcaagagga ttctctatgc
      481 acttgctcct gtgttttggg cgagcaacct gctactcgtc tggtcaccaa gtccggttct
      541 tctggtgttt gcaggcatgc tgcaggggac agtctcgatg accctggttg taacccaggc
      601 catggccttc gagctcgttc cctcaagcag aatgagcgat tggctggctg ttctgcggtt
      661 ctttaggatg ctcgtcggcg ccgccctggc cttcgtgtcg gggatcatat gggatcactt
      721 cggccccgag tacgtgttct tgcttgctgt cggtatagac gcggcgattc gcatcccact
      781 gcttgcgggg ctaccggaga cgctcgacgc gcccgtgtag tcggaacttc atcatgttgc
      841 cacttcggcc tcggcaacaa atacctccag aggcgagctt ctgcgcctaa tccacctgcc
      901 tatagacgtg ccattggcgc cgctcgctgc ccctatccag gtatatttcg tatgtatgtc
      961 cgttatcgca gagtaccctg tagtagttgc ggtggcgcct agtcctccat gtcctggcag
     1021 agggatgagt acctccgaaa ccgaaatcct gccactcaga gagaatctcc actacttgca
     1081 cggtggtgtc gttccacgcc agcgccgtcg gcgcggttcc gcccggcgag cactcaactt
     1141 tcgcgggttt tccgatgaaa taaggcacca acatcacccc attggtgagc aaagaagcct
     1201 caccggtatg tgccagtacg ttccgtgctg gccatgctga tcggctacac ttccgactga
     1261 ttgcactgcg cctttaggca cggtgggtta tcatcccgta cgctcatcga caactaggcc
     1321 ttgagctcga cgaagaatgt gtaccgatcc ggcctgaagt cggccctcac gaattccacc
     1381 ggataccctc cggaaatgta ggttgtgcgc cccagtcgga gcaggggagc accgggtgga
     1441 actatgaggt ggcgggcttg ttcttcagta gcggtgacgg ctgttatcac ttctgtggct
     1501 cgtgttaccg gcccgacgtg cgcctccact agacagtaca gagaaggagc ccctaggtcg
     1561 aagttctcta gaatcagccc atggtggcgg gcaaaatggg acgcgttgat agagagttcc
     1621 ttttctccgg ccagcctgag gcggacgatc ctcagaaccg gatcgcctgc cttcacctgg
     1681 agagcctgag ccacatcctc atccgctgga acccattcct tccctatcaa tctcgcgcca
     1741 ggcttgaaac cgagtatcct gatttcgtcg gagaaactgc ggagcgttac aagttgctgt
     1801 acgatcttgg gctggctcac aaagctgccc tttcctcgtt tgcggtgcac gagtccgtcg
     1861 cgggtcagtg tcaggactgc ctgccgcgct gttgtacggc tgacgccata cattgcagac
     1921 agctccgctt ccgtgggaat gggttcgccc ggctccagct ctccagcacg tattttgtcg
     1981 cgcaactggc ttgccaactg ctcataaagg ggaatcggcg agttctcatc tagccgaagt
     2041 acttcaggac acattaagct caaccctcac caatctacag gtagtaatgt catcatattg
     2101 caacatacca ttcgagacaa ctacactgac tccttcattc gctcggtgcc cattgcccac
     2161 tcacgcaggt gagtggatct gtttgcgctg ctcctcctga ggaggctggt atgtatgaga
     2221 tttttgtgct ctcgcggcag gagtttctga gggggcgtgg tatagtaaca atacaatgtt
     2281 actacattat gaatttggtt ctgatgggtg cttgcaccga gcgctgtagg aggtagtcca
     2341 catgcacgaa ccaatggaac gattccgctc cgttttttct gaacccaaac ccgtaatcgg
     2401 catggtacat ctgccacctc tgcctggggc tccacgctac aacggggccc agatggcaga
     2461 catcgcggag tacgcgatca gcgaagcccg aaagctggag gagacgggct atagtgggct
     2521 gatagttgag aacttcggag atgccatgtt ctacaagaga gctcgtccgg agacgattgc
     2581 ggcgatgact tacgtggcta aggcgcttgc agatgcggtc agcttgccta tgggcatctg
     2641 cgtgctgcag agcgacgcga tagctgcgat atccatcgcc catgtggtcg gcgctcggtt
     2701 tgtacgtgtt ccttactaca ccgaaaccta cgtggtcgac gcagggctca tggagtcttg
     2761 cgccgcggac gcgctgcgct ttaggaagct tctggggtcg gatgctctca tcttcgcaga
     2821 tgttcacata aagcatgggt acccattgtc tcagcggccg atacaggaat cggccgaaga
     2881 cgctgtccat agaggactgg cggatgttgt catagttact gggaagaaga ccggcggacc
     2941 gactaatgtt gaagatgtcc agaaagtgaa aacgcagatc ccccagtacc cagtgcttgc
     3001 cggaagcggg ataagcactg ccgatgctca catactgctc ggcatcgccg acggagccat
     3061 cgtcggcacc aacctgaaga tcaatggcga tactgaaagc cccatagatc tgtgcagggc
     3121 gaaagagctt atgacggtaa ttcaggggat caggcagcag gaaggggatg accagcggtg
     3181 aaatctagac ttacggcgtt caacgagatg ttcgggcaca aggcgataat cggtatggtt
     3241 cacctgccgc cattgcccgg ctctccgctc tacgatgaca agggaatatc ttcgacaatc
     3301 gaattcgcaa tggacgaggc caggaagctc caagatgggg gcgtagatgc gatagagatc
     3361 gagaactacc ttgacgccag ctacttcccg gatgccgtgg ggcctgagct ggtatcggcg
     3421 atggccatca tcgcccacga agtccgaaag gttactgata ttcctatggg catctgtatc
     3481 cttgcagatc ccaccgcagg tctggccgtg gcccacgcaa ttcgagcaca gttcgtaaga
     3541 gctaccttct ttaccgaggc ctccgtggac gtgtcagggc tagttctggc acgcccacat
     3601 caactgctgc gctaccggaa gttcctcgac ccgtcggtga aaatcttcgc ggacgtgcac
     3661 atcaagcact ccgctccgct cgcgccgcgg ccgatagagg aatccgcgta cgatgcggcc
     3721 tacttccagg cggatgcagt gatagtcagc ggcaagcaca cgggctttcc aacacgcatg
     3781 gatgatgtgc gcgctgtgaa gcgagtattg cctgaggtgc ccgtcctcat cggcagcggt
     3841 ctgcggcaat cgacagcggc tgagtttttc gaagtggccg acggagccat agttgggtca
     3901 tcgct
//
LOCUS       DAOTAC010000248         3888 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_18872_length_3888_cov_3.514219, whole genome shotgun sequence.
ACCESSION   DAOTAC010000248 DAOTAC010000000
VERSION     DAOTAC010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18872_length_3888_cov_3.514219"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /gene="spoIIM"
                     /locus_tag="PLM74_09730"
     CDS             complement(<1..502)
                     /gene="spoIIM"
                     /locus_tag="PLM74_09730"
                     /inference="COORDINATES: protein motif:HMM:NF014052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HON43391.1"
                     /translation="MARKRYPEKLAFGYIRANAGCYALAFVMFVAGVCTGAVYASAIQ
                     EGSTAISSYLESGLADLRRAAPGSHGSAMLASVNANLCSAAFVWLAGLSVVGFTGALL
                     ILFVRGFSIGFTVAFLVRQMGGAGAVLALSSVLPHNLVAVPALLTACATSVGFAWSVV
                     KKSLLGI"
     gene            complement(558..1775)
                     /locus_tag="PLM74_09735"
     CDS             complement(558..1775)
                     /locus_tag="PLM74_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019687888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease Q family protein"
                     /protein_id="HON43392.1"
                     /translation="MVDGGDRHLVKEYFVDLHVHIGRDSRGRPVKVSGARDLTFASIA
                     REAGERKGIDIIGVVDCASGRVLSDIDCLLQSGEMEECPGGGIRYMKRTVILPGVEVE
                     AACRANEGPSAHYIAYFATLPQVREAARELSRHMTNIDLSTQKARLTPEQLARLVGDM
                     GGLFIVAHAFTPHKSLYGTCARRITEVFGAEARRMVSAVELGLSADTSMADRIAELAS
                     YTFLSNSDAHSLGRMGREYNVMLMESPSFAEVSLALARIDGRRVVANYGLDPRLGKYY
                     LSACEVCGTRFHAPNAALHCPECGAYRVVRGVSDRVDEIADYPEAKSPAHRPPYRHQV
                     PLQFVPGVGRATIARLIGAFGSEMAVLHRATRDQLVEAAGERIADRIVAAREGRLTIQ
                     AGGGGTYGRTLPD"
     gene            complement(1768..2313)
                     /locus_tag="PLM74_09740"
     CDS             complement(1768..2313)
                     /locus_tag="PLM74_09740"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HON43393.1"
                     /translation="MFREELVERRQVYSGKIVTLNVDTVELPNGGLAGREVVVTRDSV
                     GIAPVTHDGDLVMVRQYRHAGGVELLEIPAGRIDAGESPEDAVLRELGEETGYKAIEL
                     TKIAGFYLAVGFATEYMHLYVALAEPGGDAHPDDDEFVQVELVPLSRIPGMIKSGEIQ
                     DVKTLAAMLHLAWTRGLAQNG"
     gene            complement(2332..3405)
                     /locus_tag="PLM74_09745"
     CDS             complement(2332..3405)
                     /locus_tag="PLM74_09745"
                     /inference="COORDINATES: protein motif:HMM:NF024384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HON43394.1"
                     /translation="MINANLARVVRVLQDSPDIQELIVDTDGGGECAAVCYPPLTGSV
                     QPGDRVIVNTTAVDLELGSGGRHFVIWALPRTRLSGHIRGHIMKMRYSPMQACTGSFE
                     EDPANHAAIDAFTGLAGMPVIICELHSMVAPVLSGFAGRAAYIMTDGAALPIAMSRAV
                     RQLKRARLVDATITAGHAFGGDLEAVNVHSALVAAKAVARCDAAVVAMGPGIVGTGTR
                     YGFSGVEQGWIADAVNRMGGRPVIVPRLSRLDPRPRHRVVSHHTLTVLRDVCCTPVTC
                     VLASDMEPGFADETRSRLCDVMDRGGHTLGYAPGRDGVELAITQELELSTMGRGPDEE
                     PEFFLTCAAAGNYARTMVISEKK"
     gene            complement(3461..>3888)
                     /locus_tag="PLM74_09750"
     CDS             complement(3461..>3888)
                     /locus_tag="PLM74_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014828203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HON43395.1"
                     /translation="YGPEMLGGTANCSVVISTESIGSPIVTEPTTLIVMNRPSLDRFE
                     QAVMPGGLLIYNSSLIDRRPERTDIRVIAVPAVEMAREAGSDRVANMCVLGAYLAATG
                     VVAYESVAECLKEVLPERRQELIPLNLKALAQGASMVKL"
BASE COUNT          763 a         1311 c         1073 g          741 t
ORIGIN      
        1 cgattccgag gaggctcttc ttcacaaccg accatgcaaa gcccactgaa gtcgcgcacg
       61 cggtaaggag cgcggggacg gccaccaggt tgtgcgggag tacagacgat aacgccaaca
      121 cagcccctgc accgcccatc tgccgcacga gaaaagcaac agtgaaccct atggagaacc
      181 ccctgacgaa caagatgagc agggcgccgg taaagcctac cacgctgagg cccgccagcc
      241 acacgaaggc cgcagagcac aggttcgcgt taacactcgc gagcatggcg ctgccgtgag
      301 atccgggagc ggcgcggcgc agatccgcta ggcccgattc cagatatgag gagatggccg
      361 tactgccctc ttgaatcgct gaagcgtaga cggcgcctgt acatactccg gctacgaaca
      421 tgacaaacgc caacgcgtaa catcccgcgt tggctctgat gtaaccgaat gcgagcttct
      481 cagggtaacg cttcctggcc acggcggaca cccccgtagg ccatccgtat gcgccgcgca
      541 gccctgctat tcctgcctca atcgggcagc gtccgcccgt aggttcctcc tcctcctgcc
      601 tggattgtga gcctcccttc cctggccgcc actatgcgat ctgcaatacg ttcgcctgcc
      661 gcctctacca gctgatcgcg ggtcgcgcga tgcaggaccg ccatttccga tccgaaagcc
      721 ccaattagcc gagcgatcgt agcccgcccg acgccgggca caaactgcaa tggtacctgg
      781 tgccggtaag gcggacggtg ggccgggctc ttcgcctctg ggtaatcggc gatctcgtca
      841 actcggtcgg acacgccacg cactacgcgg taagcgccgc attccgggca gtgcagcgcc
      901 gcattgggcg cgtgaaagcg ggttccacaa acctcgcacg cacttaagta gtacttccca
      961 agcctgggat cgaggccata gttggcgacg actctacgcc cgtcgatgcg cgcaagcgcc
     1021 agcgacactt cggcaaaaga aggcgattcc atcagcatca cgttgtactc ccgccccatc
     1081 cgcccgagcg agtgagcgtc ggagttggac agaaaggtgt agcttgccag ttcagcaatg
     1141 cggtcggcca tactggtatc tgcagaaagc cccaattcca ctgctgatac catgcggcgg
     1201 gcttctgcgc cgaacacttc cgtaattcgc cgggcgcatg ttccatagag gctcttgtga
     1261 ggggtgaatg cgtgagccac gatgaacaat ccgcccatgt cgcccacgag tcgtgccagt
     1321 tgctcaggcg tcagcctggc cttctgcgtg ctcaggtcga tattggtcat gtgccggctg
     1381 agctctcgcg ccgcctcgcg cacctggggc agagtggcaa aataggctat atagtgtgcg
     1441 cttgggcctt cgttcgcccg gcaggccgcc tcaacctcga ctcctgggag gatcaccgtc
     1501 cgtttcatat agcggattcc gccgccaggg cattcctcca tctcgcctga ctgaagaagg
     1561 cagtcaatat cgctcagcac gcgcccggaa gcgcagtcta ctacgcctat gatgtcaatc
     1621 cctttgcgct cgccggcctc gcgggcgata ctcgcgaacg taagatcgcg cgcgccggac
     1681 actttgaccg gtcgtccgcg cgagtctcgc ccgatgtgta catgtagatc tacgaagtac
     1741 tccttcacga ggtgcctatc ccctccgtca accattctgg gccaggccgc gagtccacgc
     1801 tagatgcagc atagcagcca gggtcttcac gtcctgtatc tcgccggact ttatcatccc
     1861 agggatccgc gatagcggaa ccaactcgac ctgcacaaac tcatcatcgt cagggtgcgc
     1921 gtcaccgccg ggctcggcca gagcgacata caagtgcata tactcagtcg cgaatcccac
     1981 agccagatag aaaccggcga ttttcgtcag ctcaatcgcc ttatagcctg tctcctcgcc
     2041 cagctcgcgc aggacggcat cttccggcga ttcacccgcg tctattctgc ccgcaggtat
     2101 ctccaaaagc tccacgcctc cggcatgtct gtactgacgc accattacca gatcgccgtc
     2161 atgcgtaacc ggcgcaatcc ctacagagtc gcgggttacg accacctcac ggccggccag
     2221 cccgccgttg ggaagctcca ctgtatcaac gtttagagtc actatcttgc cgctatatac
     2281 ctgcctgcgt tccacaagtt cttcacgaaa caccgacacc actcgctcac cttatttctt
     2341 ctcactgatg accatggttc ttgcatagtt ccctgccgcc gcgcatgtaa ggaaaaactc
     2401 gggttcctca tccgggcccc tgcccatagt ggacagctcc agctcctgcg tgatcgcgag
     2461 ctctacgcca tcgcgtccgg gggcgtaccc gagtgtatgg ccgccccgat ccataacatc
     2521 acacagccgg ctgcgcgtct cgtcagcgaa cccaggttcc atgtccgagg caagcacgca
     2581 cgtcacaggt gtgcagcaca catcgcgcag caccgtcaga gtgtgatggc tgaccactcg
     2641 atgcctagga cgcggatcca gcctggatag gcggggaaca atcacgggcc gccctcccat
     2701 ccggttcacc gcgtctgcga tccacccctg ttcgacgccg gagaacccat accgggtgcc
     2761 tgtgcccaca atgcccgggc ccattgcgac tacggcagcg tcgcacctgg ctaccgcctt
     2821 tgctgccacc agcgccgagt gcacgttgac ggcctccaga tcgcctccga aggcatgccc
     2881 tgcagttatc gtcgcatcga cgagtcgcgc gcgtttcagc tgacgtactg cccggctcat
     2941 ggcaatgggc agcgccgccc cgtctgtcat aatgtaggcg gccctgcccg cgaacccgct
     3001 tagcacaggg gcaaccatgg aatggagttc gcatatgatc acaggcatgc cggcaagccc
     3061 cgtgaaggca tcaatggcag catggttagc cgggtcttcc tcaaagctcc ccgtgcaggc
     3121 ctgcatcggg gagtatcgca tcttcatgat gtggccgcgt atatgaccgc tcaggcgcgt
     3181 gcgcgggagc gcccagatca caaagtggcg cccgccactt cccagctcga gatcgacggc
     3241 tgtggtgttt acgatcactc tgtctccggg ctgcacagat ccggtcagcg gcgggtagca
     3301 tacagccgca cactccccgc cgccgtctgt gtccactatg agttcctgga tgtcagggct
     3361 atcctgcaga acgcggacca ccctcgcgag gttggcattg atcatcgctc tggcctccca
     3421 ggagtattct gccccgggca ggccggatac tacgaccgca ctacaacttt accatcgacg
     3481 ccccctgcgc gagggccttc aagttgagcg gtatcagttc ttgcctccgt tcgggcagaa
     3541 cctcttttag gcattctgcc accgattcat aggcaactac gccagtggcg gccagatacg
     3601 cgcctagcac gcacatgttg gcgactcggt cagacccggc ttcccgggcc atctctacgg
     3661 caggaaccgc gatgacgcgg atatccgtcc gctcgggcct ccgatcgatc agggagctgt
     3721 tgtaaatgag caacccgccc ggcatcacag cctgctcgaa ccggtcgagc gacggcctgt
     3781 tcattacgat caaggtcgtg ggctcagtca ctatcggcga tcctatgctc tcggtagata
     3841 tcacaacgga gcagttggct gtgcccccga gcatctccgg cccatatg
//
LOCUS       DAOTAC010000249         3845 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_19121_length_3845_cov_6.052770, whole genome shotgun sequence.
ACCESSION   DAOTAC010000249 DAOTAC010000000
VERSION     DAOTAC010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19121_length_3845_cov_6.052770"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="PLM74_09755"
     CDS             <1..224
                     /locus_tag="PLM74_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43396.1"
                     /translation="GCPRTPLEPTTGERHAQNYPWIRVMHRWRSVHFIIGLVGYSMLG
                     SNKTAAREAISWLDQVCHAGEFLGTCLCG"
     gene            249..485
                     /locus_tag="PLM74_09760"
     CDS             249..485
                     /locus_tag="PLM74_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43397.1"
                     /translation="MRLTGTKRQVTSLGGLPAVRMAWDTLGMNSILLEHGFEKRSGAP
                     ATELASAVTVEHLAQAQSNVELEKAKTSAAEVPN"
     gene            1255..2424
                     /locus_tag="PLM74_09765"
     CDS             1255..2424
                     /locus_tag="PLM74_09765"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HON43398.1"
                     /translation="MKAHPSRMLIVSVAFAICAALTLLPGSGLVHSAASLPSIKIGAA
                     GPYSGDLSKIGLDGLNAIKMAVEEANAAGGVRGRKIEIVEADDAGDSSKAILVAEKLA
                     MDRAVLGVVGPMNSSTVEAALPTYQRAGLAIISQSATKPSLTETGYKVMHRICPRDDA
                     QGPASAKFVVDVLKAKNVYIIDDKTAYGQGLSDEVEAALKAAGIKTVRSQITLEDRDF
                     SPILTRVKAAKPDLLYLGLANPAQAASIIKQAAGLGISVKLMGGDGLKEKDQLIAGSA
                     GLAEGMYVTTIGRDITEVPEAAEFIRKFEGKFGAMSVFSGQTYEATNILIDAMRRAST
                     DPSKLTRALVLQALGKTAGYKGILGFPISFDSKGDVIGAGIYVYQVKGDDFVLVR"
     gene            2541..3470
                     /locus_tag="PLM74_09770"
     CDS             2541..3470
                     /locus_tag="PLM74_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017510038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HON43399.1"
                     /translation="MDLFLMQIPQQIVNGVTLGSVYALLALGYSMVYGVLKMLNFAHG
                     SVFMVGAFAGWGVLSLLARGNVLATSPALALLAMVAAGTAATALLGATIERLAYRPLR
                     GGSRMAPLISALGVSIILENAAMLLTSGRAKTYQTELIFPQTWAVHVAGATISATRIA
                     MLASSVAIMILLDMVVSRTRFGRAMRATSQDAEAAEYIGIRTTAVIAGTFIIGSALAG
                     VAGVMVGLYYTQIDFMIGFSAGLKAFTAAVLGGIGNFRGAAAGGLLLGLAESIGVLIA
                     PPVYKDAIVFAVLIITLILRPSGLLGKQMRARA"
     gene            3467..>3845
                     /locus_tag="PLM74_09775"
     CDS             3467..>3845
                     /locus_tag="PLM74_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43400.1"
                     /translation="MKHAAMRQMEAEGEMGLKTMPDKAIPRTCATRQGLQTDRARRAA
                     SLPRVLVFAAYAVLLPLPLLLRDYYLIRAGGSVGIYLILAAGLSIVAGQAGLLDLGYA
                     GFYGIGAYVYALLASPKFGLHSAF"
BASE COUNT          778 a         1141 c         1200 g          726 t
ORIGIN      
        1 caggatgccc cagaaccccg ctagagccca ccacagggga acgtcacgcc cagaactacc
       61 catggatacg ggtaatgcac agatggaggt cggttcattt cattatcggc ttggttggct
      121 acagtatgct tgggtcgaac aaaaccgcag ctagagaagc gatctcgtgg ctcgaccaag
      181 tatgccacgc gggcgagttt ctgggaacgt gtctatgcgg ctgatgtgtc tatgcggctg
      241 atgtgtctat gcggctgacg ggcacgaaac gtcaggttac ctcattggga ggccttccgg
      301 cggttaggat ggcttgggac acgcttggca tgaactccat cctcctagag catggttttg
      361 agaagcggag cggagcgccg gctacggagc ttgcttctgc cgtgacggtt gagcatctcg
      421 ctcaagccca atccaatgtc gagttggaga aggcgaagac ctctgcagct gaggtaccaa
      481 actgaccaga cctgtccatt tcatagtacc ccattgcttc cgagcaggac agggccctgt
      541 caagcgcctg gtttcgcggc tctcggcgcg acatgatgcg cagcggcggc actccgccca
      601 aaagggccat acacagcgct gtaagcggca gatggcacag gcccccaagc ggctcacgca
      661 ccggcaggtc acacagtcag cgctagctta acgggctctg tcgagtccaa aaatgaccat
      721 tgacaaccgt ctcagttcat gttattcctt tagcaaggta tcacgaccgt cttacagctg
      781 aatcacgtgc aagcacaaag acgttgaagg ggaagatcgt cgcagctaaa ccttcccaga
      841 gcgagcgggg gagagtgcaa gccccgcggg gagagtgtgg cgagtgggcc ccggagtcgc
      901 aggccaaacg gccgacgcgc acacatgaat agtgtcgtgc agcggcgccc agtaggccga
      961 gccggaagcc tccgttatcg ggcaggggca tcgcctcggc gttaggccgc gatcgcgcag
     1021 atgcgcagcg caactatatg cgcttcgcag gtgcgcctcg cgtgcagccg gcaagcagca
     1081 ggccgtgctc tggaatgaga ggggcagtat atctgtccta ctagggtggc accacgagtg
     1141 taaagctcgt cccttgcggg cgagcttttt gtgttccgcc gccacagagc accgagcgcg
     1201 aatcccaggc atttcgggaa tctcaagaac ttcgagcatg aggaggcaac caaaatgaag
     1261 gcgcatccaa gtcgtatgct catcgtgtct gttgcgttcg cgatctgtgc agcgctaacc
     1321 ctacttcccg gctccggtct cgtgcacagt gcagccagtc ttccttccat caagataggc
     1381 gcagcggggc cctactcagg cgatttgtcc aagatcgggc tcgacggact gaacgccatc
     1441 aagatggctg tggaggaggc caatgccgca ggtggtgttc gcggcaggaa gatcgaaatc
     1501 gtggaggcgg acgacgcagg cgactcatcc aaggccatac tcgtggcgga gaaactcgcc
     1561 atggaccggg ccgtactcgg agtagtcggt cccatgaaca gctctacagt ggaggccgca
     1621 ctgcccacct accagcgggc cgggctggcc atcatcagcc agtccgcaac caagccgtcg
     1681 ctgactgaaa cgggatacaa agtcatgcac aggatatgcc cccgtgacga cgcacagggc
     1741 ccggcatctg ccaagttcgt agtggacgtc ctgaaggcga agaacgtcta catcatcgac
     1801 gacaagaccg cctatgggca ggggctgtca gacgaggtgg aagcggcgct taaggccgcc
     1861 gggatcaaga ccgttcgtag ccagatcacc ctggaagaca gggacttctc gcccatcctt
     1921 acgcgtgtca aagccgccaa gccggacctg ctctatctgg gcctcgctaa cccggcacag
     1981 gctgcgtcca tcatcaagca ggctgccggg ctcgggatct cggttaaact catgggcggt
     2041 gatggactga aggagaagga ccagctgatc gcaggttcgg cgggacttgc agaaggaatg
     2101 tacgtgacaa cgataggccg cgacataacc gaggtccccg aggcggctga gttcatccgc
     2161 aagttcgaag gcaagttcgg cgcgatgagc gtgttctctg ggcagactta cgaggccact
     2221 aacatattga tagacgcaat gcgccgcgcg tccaccgacc cgtccaaact gacacgcgcc
     2281 ttagtcctgc aggcgctggg caagacggca ggttacaagg gtattctcgg cttcccgatc
     2341 agcttcgact ccaaaggcga tgtgatcggg gccggcatct atgtctatca ggtgaagggc
     2401 gacgatttcg tgctagttcg ctagtgtcag ccagcgaatg ccgaccggat agcttcgtct
     2461 ggccggtgca ggtcggccta tgccggccga tgccggccaa ccgccaaccc aaatgtcttc
     2521 ggggcaacga ggtgtgaagt atggacctgt tcctcatgca gataccccag cagattgtga
     2581 acggggttac gctaggcagt gtgtacgccc tgttggcgct gggctactcc atggtgtatg
     2641 gagtgctcaa gatgctcaac ttcgcccatg gcagtgtttt catggtaggc gccttcgcag
     2701 gctggggcgt gctgtcgctg cttgcgcgcg gcaacgtgct ggccacgagc cctgctctgg
     2761 cgctcctggc catggtcgca gcaggcaccg cagccaccgc attgctgggc gcgacgatag
     2821 agcgtctggc gtaccgccca ctgcgtgggg gatctcgcat ggctccgctc atctcggccc
     2881 tgggagtatc aatcatactg gaaaacgcgg caatgctcct cacgtcagga cgagccaaga
     2941 cttatcagac cgaactgatc ttcccgcaga cctgggcggt gcacgtggcg ggggcgacca
     3001 tctccgccac gcgcatcgcc atgctggcct cgtcggtggc aatcatgatc ctcctggata
     3061 tggtagtgtc gcgcacccgg ttcgggcgag cgatgcgcgc gacgagtcag gatgccgagg
     3121 cggctgaata catcggcatc cgcaccaccg cagtgatcgc aggaacgttc attatcggat
     3181 ctgcactggc aggagtagcc ggagtgatgg tcggcctcta ctacacgcag atcgacttca
     3241 tgatcgggtt ttccgccggg ctcaaagcgt tcaccgccgc tgtgctcggc ggaatcggca
     3301 acttccgggg cgctgccgca ggcgggctgc tcctggggct cgccgaaagc atcggcgtgt
     3361 tgatcgcgcc gcccgtgtac aaagacgcaa tcgtgttcgc agtccttatc atcactctga
     3421 tcctgcgccc gtccgggctg ctcggcaagc aaatgagggc gcgggcgtga agcacgcagc
     3481 catgcggcaa atggaggctg aaggtgaaat gggcttgaag accatgccag acaaggccat
     3541 accacgtaca tgcgcgactc gccaggggtt gcagacagat cgagcgcgcc gcgcggccag
     3601 cctcccgcgt gtactcgtct tcgccgccta cgccgtattg ctgccattgc cgctgctcct
     3661 cagggattac tacctcatcc gggcgggcgg gtcagtgggc atctacctta tcctggccgc
     3721 ggggctttcc atcgtcgcgg ggcaggcggg cctactcgat ctgggatacg ccggcttcta
     3781 cggtatcggc gcttacgtct acgcgctgct ggcatcgccc aagttcgggc tgcattctgc
     3841 gttca
//
LOCUS       DAOTAC010000250         3840 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_19148_length_3840_cov_4.413738, whole genome shotgun sequence.
ACCESSION   DAOTAC010000250 DAOTAC010000000
VERSION     DAOTAC010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3840
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19148_length_3840_cov_4.413738"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="PLM74_09780"
     CDS             <1..681
                     /locus_tag="PLM74_09780"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HON43401.1"
                     /translation="KAIIITPTPSGSLRTSHSVSLTGLREKTTYYYQVVSSDAAGNVA
                     VMSGFSFTTGGTTPPPPPPPPPTPGTVNVNWALASNGGKATASSYAPATSEGPASPPS
                     NAIDGNARTYWEASKAGVGSSIEWIMVEFAKSQPINYIKTMSDVGHYPMEMTVEVEQD
                     GRWVAVGSFKSTSEQKNLMSRSGSTISFEFKFAQVVASKVRLTIRRVSNPTHAIQFYE
                     VIAMNTGK"
     gene            802..2196
                     /locus_tag="PLM74_09785"
     CDS             802..2196
                     /locus_tag="PLM74_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HON43402.1"
                     /translation="MGQAGYDVVIVGAGPAGIFAALELSNSSNLRVLMIEKGRGIAGR
                     VCPSKERRVNCVRCDPCGVLCGWGGAGAFSDGKLTLSTDVGGMLENYVGREELLRLID
                     QVDRVYLDYGAPEDVWGEEDEKVSELRDRAKLAELTLIPARIRHLGTGRTGHILEGMR
                     RALEGKVDIDLNTGAVRVSARDGVVEGIETSDGRFIPAKYVIVAPGREGASWLAEQAS
                     ALGLRTAVNPVDIGVRVELPAAVMEHITDVVYESKFVYYSRTFDDKVRTFCMCPYGEV
                     VTENNAGLMTVNGHSYAERRTENTNFALLVSKNFTEPFKEPILYGKYVAGLANLLGGG
                     VIVQRLGDLINGRRTTTDRLRKGTVTPTLEDATCGDLSLVFPYRHLVDITEMLQALDK
                     IAPGVYSRNTLIYGVEVKFYSNRLELSGDLETQVANLFAAGDGAGVTRGLMQASASGV
                     VAARGILARAAGRA"
     gene            2199..3419
                     /locus_tag="PLM74_09790"
     CDS             2199..3419
                     /locus_tag="PLM74_09790"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HON43403.1"
                     /translation="MGGNIGGDSGMVKLKPGRDARARAGHPWIYEGEISRIRGNPPDG
                     SIVTVCAGSGAFIGRGYINSASQITVRLLTWRDEPVDNEWFARRLQDAFRYRDLVAPG
                     VHSCRLVHSEGDGLPGLIVDRYEGCLVFQFLTLGMDIRKDALVRTSLELDGLKDAYER
                     SDVRSRQYEGLEQRSGFLGDPFDASSIQIRENGFALDVDVAGGQKTGHFLDQRENRAA
                     LAPYCRGARVLDCFCHTGAFAVHAAGYGAREVLGIDISEQALETARRSAALNRLDGVC
                     TFQAGNVFDTLRQMADDATARRAPEGRFDMIILDPPAFTKSRATVESAARGYKEINLR
                     AAMLLGEGGFLVTSSCSHHVDEPTFVAIVESALADAGRRARLVEARTQAKDHPVIAGV
                     PETRYLKFLVFRMM"
     gene            3494..>3840
                     /locus_tag="PLM74_09795"
     CDS             3494..>3840
                     /locus_tag="PLM74_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43404.1"
                     /translation="MRRPIKITRRQALVETGTPEALDPREDTTAPHPLKLDAYARFDD
                     VEEQVSLISTALGSPSDLAVRRISDGIVCLFLSPLVDQGAVRHSLVEPLSRAGWLELQ
                     EKGEAARVLPAAG"
BASE COUNT          778 a         1038 c         1296 g          728 t
ORIGIN      
        1 aaggcgatca taatcactcc gacgccgtca ggctcgctcc gcaccagcca cagcgtgagt
       61 ctgaccggct tgcgcgagaa gaccacctac tactaccagg tggtttcaag cgacgctgcc
      121 ggcaatgtgg cggtcatgtc gggcttctca ttcacgacag gcggcactac gccgccgcct
      181 ccaccgccgc ctccgccgac tcctggcaca gtcaatgtga actgggcact ggcatccaac
      241 ggcggcaagg ctaccgcatc gagctatgct ccggctacgt ccgaggggcc ggctagcccg
      301 cccagtaatg ccatcgacgg caacgccagg acttactggg aggcgtccaa agcgggggtg
      361 ggaagcagta tcgaatggat catggttgag ttcgcgaagt ctcagccgat caactatatc
      421 aagaccatga gtgatgtggg ccactatcca atggaaatga ccgtcgaggt ggaacaggac
      481 ggtcgctggg tggcagtagg gtcgttcaag agcacatctg agcagaagaa cctcatgagc
      541 aggtccggat cgactatcag ctttgagttc aagttcgctc aggttgttgc aagcaaggtg
      601 cgccttacca ttcgccgcgt gtccaatccg actcacgcta ttcagttcta tgaagtgata
      661 gcgatgaaca ccggaaagta ggctgagcag gttgacctgc acagcacggg ttgttgcgaa
      721 tgatcgctga ttgacacata gggccgactg ccgcatcctg gcgtgggtgc ggcagtcggg
      781 cgtcacggag gtatcaggca gatgggacag gcgggctatg atgttgtcat cgtcggcgca
      841 gggccggcag ggatattcgc tgcgttggag ctgtcgaatt cgagcaactt gagggtgctg
      901 atgatcgaga aagggcgcgg aatagctggt cgcgtatgcc catctaagga gcgccgggtc
      961 aactgcgtcc ggtgcgatcc ctgcggtgtg ctgtgcggat gggggggagc cggggcgttt
     1021 agcgacggca agctgaccct gtcaacagat gtcggaggca tgctcgaaaa ctacgtggga
     1081 agggaagagc tgctccgctt gatagaccag gtggaccgcg tatatctcga ctacggtgct
     1141 ccggaggacg tgtggggcga ggaagacgag aaggtatcgg aactgaggga ccgagcgaag
     1201 ctggcagagc tgactctgat acccgctcgc ataaggcatc tgggcaccgg tcgcaccggc
     1261 cacatcttgg aaggcatgcg cagggctctg gaaggcaagg tggacatcga tctgaacacc
     1321 ggcgcggtgc gggtatcggc cagagatggg gtagtcgagg ggatcgaaac cagcgatggg
     1381 cggtttatcc cggcaaaata cgtgatagtg gcccccgggc gcgaaggcgc gtcgtggctt
     1441 gcagaacaag catctgcgct cgggcttcgc accgcggtga accccgtaga catcggcgtg
     1501 cgggtggaac tcccggccgc tgttatggag cacattaccg atgttgtcta cgagtcaaag
     1561 ttcgtgtact actctcggac cttcgacgat aaggttcgca cgttctgcat gtgtccttat
     1621 ggagaggtgg taactgagaa caatgccggc ctgatgacgg tgaacggcca tagctatgcc
     1681 gagcgcagga cggagaacac caacttcgcc ttgctcgtaa gcaagaattt cactgagccg
     1741 ttcaaggagc caatactgta cggcaaatac gtggccggcc ttgcgaacct attgggcggc
     1801 ggcgtgattg tgcagcgact gggggacctg ataaacgggc gccgcactac aacggatagg
     1861 ctccgaaagg gaaccgtgac gcctacgctc gaggatgcta cctgcggtga cctgagcctc
     1921 gtcttcccat accggcatct tgtcgacatt acagaaatgc tgcaggcgct cgacaagata
     1981 gcgcctggcg tgtactcaag aaacaccctg atctacgggg ttgaggtgaa gttctactcc
     2041 aaccggcttg agctcagcgg cgatctggag acgcaggtag cgaacttgtt cgccgccggc
     2101 gacggtgccg gcgtcactcg cggcctgatg caggcgtccg cgtccggagt ggtggctgcg
     2161 cgggggattc tggcacgcgc cgcagggagg gcgtaggcat gggcggcaat atcggcggcg
     2221 actcgggcat ggtcaagctg aaaccgggca gagacgccag ggccagggcc ggtcatccgt
     2281 ggatctacga aggagagatc agccgcattc ggggcaatcc gcctgatggc agcatagtca
     2341 ccgtctgcgc cggatcagga gcgttcatcg gtcgcggcta catcaacagc gcctcgcaga
     2401 ttactgtgag gcttctgacg tggagggacg aacctgttga caacgagtgg tttgcccgtc
     2461 ggcttcagga cgccttccgc tatcgcgatc tcgtggcgcc gggagttcac tcgtgcaggc
     2521 tggtgcattc ggagggcgac ggccttcccg ggctcatagt ggacagatac gagggatgtc
     2581 tggtattcca gttcctcact cttgggatgg atatccggaa ggatgctctg gtgcgaacct
     2641 cgctcgaact tgacggcctt aaggatgcat acgagcgcag cgacgttcgg agccggcagt
     2701 acgagggtct cgagcagcgg agcggttttc tgggcgatcc attcgacgcc tcctcgatcc
     2761 agattcgtga gaacggcttc gcgctggatg tagatgtggc cggcggccag aagaccgggc
     2821 acttcctgga ccagcgtgag aacagggcgg cgctggcgcc gtactgtcgg ggagcgcgcg
     2881 tgctggactg cttctgccac acaggggcgt tcgcggttca cgcagccggg tacggggcgc
     2941 gggaagtcct gggaatcgat atctccgaac aggcgctgga aaccgcacga cgaagcgcgg
     3001 ctctgaaccg gctggacggg gtatgcacat tccaagcagg caacgttttc gatacgctca
     3061 ggcagatggc tgacgatgcg accgcgcggc gggcgccgga ggggcggttc gatatgatca
     3121 tcctggatcc tcctgcgttc accaagtcgc gggcaaccgt ggaatcagct gctcgcggtt
     3181 acaaggagat aaacctgcgc gcggccatgc ttttggggga aggcggcttt ttggtcacgt
     3241 cgtcatgttc gcaccacgtg gatgagccca ccttcgtagc gattgtagaa tcagcgctag
     3301 ctgatgcggg taggagggcg aggttggtgg aggcacggac gcaggcgaag gaccaccctg
     3361 tgatagcagg cgttcccgag acccggtacc tcaagttcct ggtttttcgg atgatgtaga
     3421 ggccttagcg gttcacaatg ggcaatagag gggactataa ccctgatgtg aaccattgag
     3481 gcaggtcgtc gaaatgcgta ggccgatcaa aatcactcgc agacaggcgc tggtcgagac
     3541 cggcacgcct gaagcactgg atcctcggga ggacactact gcgccccacc ccctgaagtt
     3601 ggacgcgtac gcgcggtttg acgatgtcga agagcaggtc agtctgatca gcacggcgct
     3661 gggcagtcct tctgatcttg cggtcaggag gatctcggac ggcatcgtat gcctgtttct
     3721 gtcgcccctc gttgatcagg gggctgtccg gcacagcctg gttgagccgc tcagcagggc
     3781 cggctggctc gagctgcagg agaagggcga ggcggcgcgg gtgttgcctg cggccggttt
//
LOCUS       DAOTAC010000251         3833 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_19191_length_3833_cov_4.047380, whole genome shotgun sequence.
ACCESSION   DAOTAC010000251 DAOTAC010000000
VERSION     DAOTAC010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19191_length_3833_cov_4.047380"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="PLM74_09800"
     CDS             complement(<1..259)
                     /locus_tag="PLM74_09800"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HON43405.1"
                     /translation="MLQVDLHGMKTAEAIACFTRAYDSALGSGTRSDACLKVIHGYGS
                     GGAGGDTRRAVRSLLRANPSCADYVTGEDIDGNPGYTVVYPR"
     gene            complement(450..884)
                     /locus_tag="PLM74_09805"
     CDS             complement(450..884)
                     /locus_tag="PLM74_09805"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HON43406.1"
                     /translation="MANVTFGADVVWSGSGLYCEGNARGFKVAVDEPKELGGADRAMN
                     PVELLLCSLGGCMSKCAAAFARGCGVELKGFRVELEGDLDPDGFLGKNKNVRTGYQEI
                     RYRMIIDSPSPQANIEKLRGMIEQRCPVSNTLCGVKVSKIGS"
     gene            complement(1307..1382)
                     /locus_tag="PLM74_09810"
     tRNA            complement(1307..1382)
                     /locus_tag="PLM74_09810"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1347..1349),aa:Thr,seq:cgt)
     gene            complement(1951..2025)
                     /locus_tag="PLM74_09815"
     tRNA            complement(1951..2025)
                     /locus_tag="PLM74_09815"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1991..1993),aa:Gly,seq:ccc)
     gene            2890..>3833
                     /locus_tag="PLM74_09820"
     CDS             2890..>3833
                     /locus_tag="PLM74_09820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HON43407.1"
                     /translation="MNARLLAERYERYVIDLRREFHRHPEPSGEEKRTSSRVIAELES
                     IGIPYEVVGKYGILATLRGSGSAGSEVMLRADMDALPIQESDSNLACPKAVVSGISGC
                     AHLCGHDAHTAMLLGAARGLAETRTELPGDVLLCFEQAEETTEGIAPMMLALEGKKPK
                     AVWAIHVYSELPSGAISVESGPRMSGMSAPTVTLRGSGGHGSRPDQTIDPLLCAAQAV
                     TDLCSIPAREVKPTDVGVVTIGMIRAGEVGNVIPASASFAGTARFFDPNLGERIIESV
                     ERIVTHTAAAHRCQASVTVNGPHPAVINDAALSALAARS"
BASE COUNT          810 a         1159 c         1113 g          751 t
ORIGIN      
        1 gccgcggata gaccacggtg taccctgggt ttccgtcgat atcctcccca gtaacataat
       61 cggcacaaga cgggttggcc ctaagcagcg agcggaccgc acgacgtgta tcgccgcctg
      121 ctccccccga tccgtagcca tggatcacct tgaggcatgc gtcggaacgc gtgcctgagc
      181 cgagggcgct gtcgtatgcc cttgtgaagc aagctatggc ttctgctgtc ttcattccgt
      241 gaaggtctac ctgtagcatg ataaccgccc cacccggcaa aatgccatgg agaaggatga
      301 aacgaaaagg ggtgacgcag cacacctcag gaactatggg ccctgcggcg acgacacccc
      361 ctacccgtat cactctcatt gcctatacta acatacatgg cgcaaggcag caatgacccg
      421 cgagtcattg actcccgagt tactacccat caagacccaa tcttggagac cttcaccccg
      481 cacagcgtat tcgataccgg gcaacgctgc tctatcatgc cgcgcagctt ctcgatattg
      541 gcctgtggcg atggagaatc aatgatcatt ctgtacctga tctcctgata accggtacgc
      601 acgtttttat tctttcccag gaagccatcg gggtcgagat cgccctcaag ctcaacgcga
      661 aaccccttca gttccacacc gcacccgcgt gcaaaggccg cagcgcattt gctcatgcat
      721 ccgcccagag agcagagaag caactccacc gggttcatcg ccctgtccgc gccgcccagt
      781 tccttcggct cgtccactgc gactttgaac cctctagcat tgccttcgca atacagacca
      841 cttcccgacc agaccacgtc cgctccgaac gtcacgttcg ccacctgtat tcaccccttg
      901 cccgatcaat gtgatgtgat ggtagtatgt ccagggcgga tagtccaatt gctcttcact
      961 ccacttggcc accggcatcc cctccggcgc catcgcccgc cgccatacgc actgaacccg
     1021 gcagcttcgt tccgctgatc gatgtcggca cattggccta ccgccgtaag tatgacaatc
     1081 acgctgtgaa gaagacgctc acaatcccag cgtggctcaa cacgatggcc gagagcaaca
     1141 acactaactt cgacggtctc tcgacaacca gggaaacggg aatagtaagc aaaaataaaa
     1201 cgcccacagt tgggcgtttc ctgttctccg tgccaggccc gcgtctgttt gggagctgga
     1261 ggctgcgatt tctgtcatcc gtcacagact ctagctggga gtataatgga gccggcgaag
     1321 ggaatcgaac ccgcgacctg cgcattacga gtgcgccgct ctaccaactg agctacgccg
     1381 gcccacaaat ctcgcgcaag tgttatgtta tcagctccgc atgtgtgtgt caatcacccc
     1441 catctccact cctccatctc cacccctcca tctctcggga cgcccaacat atcgccgaca
     1501 tgtagcaggg accggagcgg atcggcggac ctatgccctg taggctagga tttccgaggg
     1561 gcttaggacc acctcctcag cctacgcgcc ctagcttggg ttttcgtcgc ttcgggtgtc
     1621 acgtggcgag gcgaccgccg aaggccggac tatcacgaat tctcattgca gcccgggcaa
     1681 ggtgcagtat tctgacttgg ggcgcagaga tttgcgctct tcgctgtcta gaggggtggc
     1741 tttctcccga tgcatgcgaa aagcccccct tggacgcctc caaaggtgca aaggactgca
     1801 cacacgcgtt atgcatacgc tatgaccaat tcgactcagg cttctagcgt tctccttcgg
     1861 gggtgaagag ggtcttccgg taagcagatc tcttgcgggg cggggtttct gcaaggcggg
     1921 tatcctgcaa agcgcggcag tacatgaaaa tggagcggat gacgggattc gaacccgcga
     1981 ctcacggctt gggaagccgt tgccctacca ctgggctaca tccgctcgca aagcttcgta
     2041 actacgctct tagtatacgc aagtgtggcc aaacgcgcaa gggggggtcc aggaggggtc
     2101 aggttcccgt caaagtccgt ctcacggctt gagcgcatgt gcgaaggtct gtatggtgtt
     2161 gcgacgttgc gatgttgcga tgttgcgacg ttgcgatgtt gcgacgtgtc cacaggcggg
     2221 cgaggcagcc ggaaccatat ggggtggatc gagggaaccg gcggggagtc gacggggttt
     2281 tcggggcctg ggtcgcagcc tgtcgtgtgg ccgacaggat ttttgcacga cctgtcgggg
     2341 tggctacagg atttgcacgg ggcctcaaga cagtagagaa acctggcgcc ccgcattcac
     2401 cagctggaag gacggccggc gaaactgcag ctaaacgcgg cgagcgaatt gggcggttac
     2461 ggcgctaata gtgcgaaagg gcccaagatc gtgtctgcgc cccgcaattc ccacctattg
     2521 cctttagttt ccgtcgaacc tgtcgtccag ccgacaagta tcccggaaaa cctgtcggcg
     2581 gggcgacaag ttgtcctcag cctgctccgc ctacgcgtcg atgactcgtt ggccatggcc
     2641 ctacgcgcgg ccccagcgtc accatcaaag ccctcttccc agtcttggac gggcgcggac
     2701 gggcgcggac gggaacgggc gggaacggac ggtcgcagga tggtgcaaga tggtgcaaga
     2761 tggtgcaaga ttccgatgtg atgaaggatt tccggactcg gtgtcaaact cccagcctgc
     2821 tacggctaaa ggatgtggga aagccggggc aagtgattga atccaatatc gcgcaatcga
     2881 ggtagagata tgaacgcgag actacttgcc gagcgatacg aacggtacgt gatcgacctc
     2941 cgaagagagt tccacaggca tcctgagccg agcggagagg aaaagcgcac gtcatcccgg
     3001 gtaatcgcgg agctggagtc tataggtatt ccatacgaag ttgtgggcaa atacggaatc
     3061 cttgccacgc tgcgcggctc tgggtcggca ggcagcgagg tcatgctccg cgccgacatg
     3121 gatgctcttc cgattcagga atctgacagc aatctagcct gcccgaaagc ggtcgtgtcg
     3181 ggcatatccg gatgcgccca tctgtgcggg cacgatgcgc acaccgcgat gctgctgggc
     3241 gcagcccgag gtctcgctga aaccagaact gagcttcccg gcgatgtcct gctctgcttc
     3301 gaacaagccg aagagacgac agaagggatt gcccccatga tgctggctct cgagggcaag
     3361 aaacccaaag cagtatgggc gattcacgtc tactcggagc tgccgtcggg tgcaatatcg
     3421 gtggagtcgg gcccgcgcat gtcgggcatg agcgccccca ctgtgacctt gaggggcagc
     3481 ggaggccacg gttcccggcc tgatcagacc atcgatcctc tcctctgcgc cgcacaggca
     3541 gtgaccgatc tgtgctcgat acccgcaaga gaagtgaagc caacagacgt cggcgttgtg
     3601 accatcggga tgatcagggc aggcgaagtg ggaaacgtga tcccagccag cgcctcgttc
     3661 gccggaacgg ctcgattctt cgacccgaac ctgggagagc ggattatcga gtccgtcgag
     3721 cgaatcgtga cgcacactgc tgcagctcac cgttgccagg caagtgtgac cgtaaacgga
     3781 cctcatcctg cggtcatcaa tgacgccgca ctgtccgcgc tggcagccag gtc
//
LOCUS       DAOTAC010000252         3777 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_19564_length_3777_cov_4.232402, whole genome shotgun sequence.
ACCESSION   DAOTAC010000252 DAOTAC010000000
VERSION     DAOTAC010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19564_length_3777_cov_4.232402"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            204..279
                     /locus_tag="PLM74_09825"
     tRNA            204..279
                     /locus_tag="PLM74_09825"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:237..239,aa:Val,seq:cac)
     gene            complement(250..1095)
                     /locus_tag="PLM74_09830"
     CDS             complement(250..1095)
                     /locus_tag="PLM74_09830"
                     /inference="COORDINATES: protein motif:HMM:NF039343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HON43408.1"
                     /translation="MFDIHSHLLPGLDDGPTRIQDSLEMLRTAQRVGVRAIVATPHCE
                     SHRLSLRTSTIQSAVTGLRTEASDDPEASAVELYVGSEIMLDPGVRAALDAGDVPTLG
                     GSTRFALVELPMREIPDYAEDVLFELAATGYVPVLAHPERNHALSQRFDVMLRIIKRG
                     ALMQLEAGSVVGAYGQIAQQCAMDMIDRRMCHFIASDAHRPGAYDALIPRAVEAVAAR
                     IGREAADALFDANPAAALAGERPVIAEPVSEEEWQEGRRRKERAGRFHILPAKKWWGK
                     QGSNL"
     gene            1421..2224
                     /locus_tag="PLM74_09835"
     CDS             1421..2224
                     /locus_tag="PLM74_09835"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004457443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HON43409.1"
                     /translation="MFKFFSKREVADDYRDSKQALVTLYNPKSAASEAYRMLRTNIHF
                     SSGGDAFKVLVITSTFPGEGKSLTAANLAITFAQAGEGVVLVDADMRRSSQHEKFEMS
                     ESPGLSEAIVSGNVEASLRDGPVDGLKLITAGITPPNPSELLHSRRMDNLLTDLKSRS
                     DLIVLDAPPVLAVADASVLASKADGAIVVVDMTMSDKNASRRAIDSLRAVNARVLGTV
                     ITGIEPVRKRYGYHNYYNGYYSGYYEGYYDDSEHSSDEHGKAHRGRTVQ"
     gene            2236..>3777
                     /locus_tag="PLM74_09840"
     CDS             2236..>3777
                     /locus_tag="PLM74_09840"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HON43410.1"
                     /translation="MDLKTYRIIHRESPRARWSVPTIISFCAILLLAAMSLGPIPAAA
                     QDQPAKASEYVLGEGDVLSISVFGDGQLSVTVPIGPDGDVSVPLAGRIKAGGRTAVQL
                     AADIASALSTYMKNPSVTVNVTQYRKIRVVVLGQVVRPGAYELPGNAGALAAIAAAGG
                     VTDMADVTGITAASAFGEAACVAYESALTNPAADVQLQSGAVIYVPRAYRQVLVMGDV
                     IRPGSYSAVGGAGQGASTTGRMTLLDAVAAAGGVRGDARRATVVWNGVRDGAASSAQA
                     NLGDLIDAPESAHNIVLEPGDVLLVHEGPEEYVSIMGEVARPGVYPLRRDATILDVIA
                     AAGGPTPRANMGRVRVYQGADVQGASTFAVASDRLSFEGDVEANPKAHAGQLIVVPSR
                     AIRVHVTGSVNRPGPVDLLQGATLADAVAAAGGLRPDADGRHAVLTRADGQDVSVSQI
                     DIESLFAGGAQGAAPVAQDGDSIFVPEAVPGQVVVLGEVARPGAYKLQPGAKLLDAIA
                     AAGGAS"
BASE COUNT          720 a         1095 c         1235 g          727 t
ORIGIN      
        1 gagaccggcg ccgaattgct gtcgggcccc ggccctgcgc tgatcgagct gtagaggcag
       61 ttgggcgacg gggcgatcag atttcggagc gatctaccga gcgatctacc gagcgatctg
      121 ccgagcgact tgcggagcga tttgccgctt gcgctctgtg tgggggcctg atataatagc
      181 tttacgcgag gcgcgcacga agtggggcaa tagctcagct ggaagagcac ctgcgtcaca
      241 tgtagggggt cacaggttcg agccctgttt gccccaccac tttttagccg ggagtatgtg
      301 gaatctcccg gctctttctt ttcgccggcg accctcttgc cactcctctt cggaaaccgg
      361 ttcagcgatc acaggccgct cgccggccag cgcggcggcg gggttggcgt cgaagagcgc
      421 gtcagcggcc tcccgaccga tccgagctgc aactgcctcg accgcgcgag ggataagagc
      481 gtcgtacgca ccgggcctgt gggcgtcgga tgcgatgaaa tggcacatac ggcggtcgat
      541 catgtccatc gcgcattgct gggcgatctg cccgtaagcc ccaacgacgc ttcccgcctc
      601 gagctgcatt agggccccgc gctttattat tcggagcatt acgtcgaaac gctgcgacag
      661 cgcatggttg cgttccggat gggcgagcac cggcacgtat cctgtcgccg ccagctcgaa
      721 cagtacgtct tctgcgtagt ccggaatttc ccgcatgggc agctccacca gcgcaaaccg
      781 ggtgctcccg cccaaggtcg gcacatcgcc ggcgtccagc gcagctcgga ctcccggatc
      841 aagcatgatc tcactgccaa cgtagagctc tacagccgat gcttcaggat cgtctgaggc
      901 ctccgtccgt agcccggtga cggcgctttg gatcgttgag gtgcgcaacg acaggcgatg
      961 cgactcacaa tgcggagtgg ctacaatggc acggacgccg acgcgctgcg ccgtcctcag
     1021 catttccagg gaatcctgga tcctggttgg cccgtcgtcc aggccgggaa gcaggtgaga
     1081 gtggatatcg aacatgtatg agtcctcctt ggctttagga tagcacggtg ggcggggcaa
     1141 aggaaggtcc ggtgggttcc atggatgttg tggaactgaa tggcatcggg aatactgtaa
     1201 aatgtgagag gtaaaccgcg gttcatatag aatagactac agggaccgac agtagctcgg
     1261 gcttcccgcg attgcccatg ataacccgct agaatgaaat gccagtgtat gtagacatca
     1321 ttgcagaaca tggtatcata gtaattgtct atggggacat gtcgatattt gtggggtgaa
     1381 tacgggctga ggggatgccg gcattcggag gaaagcctga atgttcaagt tcttcagcaa
     1441 acgagaagtc gcagatgact accgggattc taagcaagcg ctagtcactc tctacaatcc
     1501 gaagtctgcg gccagcgagg cctaccgcat gcttcgcact aacattcatt tctcaagcgg
     1561 cggcgacgca ttcaaggtac tggtcataac cagcacattc cccggcgaag gcaagtcgct
     1621 caccgccgcc aatctggcca tcacatttgc ccaggcgggc gagggtgttg tcctggtgga
     1681 tgccgacatg cggcggtcga gtcagcatga gaaattcgag atgtcggaat cgcccgggct
     1741 ttcagaggcc atcgtgtccg gcaatgtcga agcatcgctc agggacggcc ctgtcgacgg
     1801 gcttaagctc atcactgccg ggatcacccc gcccaatccg tccgaactgc tgcacagcag
     1861 gcgtatggac aacctgctca ccgacctcaa gtcgcgatcc gacctgattg tccttgatgc
     1921 accgcccgtg ctggccgttg cagacgcttc ggtgctggct tccaaggctg acggggcgat
     1981 cgtcgttgtg gacatgacga tgtctgacaa aaacgcgtcc aggcgtgcta ttgattcgct
     2041 cagagccgtg aacgcgcgtg tactgggtac agtcatcacc ggcatcgagc ctgtccggaa
     2101 gcgctatggc taccacaact actacaatgg gtactacagt gggtactacg aagggtacta
     2161 cgatgattcc gaacatagct ccgatgagca tggcaaagcc cataggggac gtacagtcca
     2221 atagaagggg atcgtttgga tttgaagacg tacaggatta tacatagaga aagtccgcgg
     2281 gcacggtgga gcgtccctac gatcatctcg ttttgcgcca tccttctact ggcggccatg
     2341 tcgcttgggc cgatcccagc tgccgctcaa gaccagccgg ccaaggcttc cgagtatgtt
     2401 ctgggagagg gagacgtcct gtcgatcagc gtgttcggag acgggcagct cagcgtgaca
     2461 gtgcccattg gcccggacgg cgatgtatcg gtgcctctag ccggtcgaat caaggcaggc
     2521 ggacgaaccg cagttcagct cgccgcggat atagcgtcgg ctttgtctac atacatgaag
     2581 aacccttctg tgactgtgaa tgtgacgcag taccgaaaga tccgcgtggt agttctgggc
     2641 caggtcgttc gccccggggc gtatgagctc ccgggcaacg cgggcgcttt ggctgccatt
     2701 gccgctgccg gcggcgtgac ggatatggcg gacgtgaccg gcataaccgc ggcgtctgcg
     2761 ttcggcgagg cggcctgtgt ggcctatgaa tccgctctta cgaatcccgc cgccgacgtt
     2821 cagctgcaga gcggagccgt catatacgtt ccgcgcgcat accgccaggt gctggtgatg
     2881 ggcgatgtga tcaggccggg ctcgtactcg gcagtgggcg gcgccggcca gggcgcctcg
     2941 accaccggca ggatgactct gctggatgct gtcgcagccg cgggcggggt tcggggcgat
     3001 gcgcgccggg cgacggtagt gtggaacggt gtgcgcgatg gagcggcgag tagcgcgcag
     3061 gcgaacttgg gcgatctcat agacgccccc gagtccgccc ataacatcgt gctggagccg
     3121 ggagatgtcc tgcttgtaca tgaaggacct gaagaatatg tgtccataat gggcgaggtg
     3181 gcgaggcccg gggtgtaccc cttgcgccgc gatgccacca tcctcgatgt gattgccgca
     3241 gccggaggcc cgactcccag ggccaacatg ggccgcgtcc gggtctacca gggcgccgac
     3301 gtccagggcg cgtccacatt tgccgttgct tccgacaggc tttccttcga aggcgacgtc
     3361 gaggcgaacc ccaaggcgca tgcgggccag ctcatagtgg tcccgtcgcg cgctatacgg
     3421 gtgcatgtga ccggatcagt caaccgtccg ggccctgtgg acttgctgca gggcgctact
     3481 ttggctgacg cggtggcggc tgcgggcggc ctgcgacccg acgccgacgg gcgacatgcg
     3541 gtgctcaccc gggcagacgg gcaggacgta tcggtgagcc agatagacat tgaatcactc
     3601 ttcgccggcg gcgcgcaggg ggcggcccct gtggcgcaag acggcgactc catatttgtg
     3661 ccggaggccg tgcccgggca ggtcgtggtg ctgggcgagg tggcgaggcc tggagcgtac
     3721 aagctccagc cgggggccaa gcttctggac gccatagcgg cggcaggagg cgccagt
//
LOCUS       DAOTAC010000253         3752 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_19728_length_3752_cov_4.593454, whole genome shotgun sequence.
ACCESSION   DAOTAC010000253 DAOTAC010000000
VERSION     DAOTAC010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19728_length_3752_cov_4.593454"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            238..1170
                     /locus_tag="PLM74_09845"
     CDS             238..1170
                     /locus_tag="PLM74_09845"
                     /inference="COORDINATES: protein motif:HMM:NF020391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sporulation protein YtxC"
                     /protein_id="HON43411.1"
                     /translation="MDCVQIGSYLAADSLRRRLFCELDHLRAQGLDISIRYRVLGDLT
                     LIECTAKSSRNTLVNDQDLRRLFRRRLANLAAGMIFDEVEEALILDTLRTRHPELEEE
                     EIAAVSEYAVALLNEGVGGVLEPSMRLREVTTDVEEYLSDSNALVIEGFIRFRMKEYM
                     EELKRSAEEALIRFMAEREHREFVRLLKYFVDIQDQRQETVHVVRTAENRFELRDSEG
                     GRVVGDYMDTLASDAPGEGVDMSDLLISALITAAPRKVVLHFEPDSTTAETIDTVFEG
                     RVERCRGKDCRVDGCRMRHPSATGEDPVTPAKRQ"
     gene            complement(1291..3033)
                     /locus_tag="PLM74_09850"
     CDS             complement(1291..3033)
                     /locus_tag="PLM74_09850"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HON43412.1"
                     /translation="MSNRSLPTRNLRLLAAVVALLFVALGVTGATVAETDRSRLVVLA
                     TSDLHGHIYPIDYFTNQADEGGLAQIYTYVKQVRSDSPNVLLVDNGDVLEGGNSVLPY
                     HYMFDSSAPNPMIKVMNLIGYDSMSLGNHEFNYGLKTLGKAISEAKFPVISGNIVRES
                     GESFIQPYMIKDFGEFKVGVLGITTPKVPTWEKPENIEGLVFMSPLETALKYIPQMRA
                     EGADVVIVVGHTGWERKPKDSGKLEAWGSPDNWVDTGQAEENWALRLANNVPGLDAIV
                     AGHDHVAVPMVAKMGDIVATDVIITQPGQWGDYVSRIDIALERGADGRWRVAERSSTV
                     VPMTGVQPAPEVLEAAKYYHDQTVDFFKSPVAQATGDMPGGPPARFYDNALVEMINEA
                     QLWATGADISIAALFTDTSRINKGPMTMQDVYGLYVYPNTLYTIKINGQQLREAIEAS
                     ANYFKTYTGQTNLDDLINTSMRGYNYDIYQGVEYTVDISKPLGQRVVTLKFKGEDVRP
                     DQEFVVALNNYRAGGGGGYTMFRNAQLLSESTTEVREIMVDYLREVGKFGPESVDNNW
                     RLLPAVVEQMARKR"
     misc_binding    3256..3518
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3641..>3752
                     /locus_tag="PLM74_09855"
     CDS             3641..>3752
                     /locus_tag="PLM74_09855"
                     /inference="COORDINATES: protein motif:HMM:NF014839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HON43413.1"
                     /translation="MESIRISLPDGSVIEAPRGTTIRDLAARLSPRLAARA"
BASE COUNT          737 a         1051 c         1148 g          816 t
ORIGIN      
        1 gttggttaga gcgcaacgtt gacatcgttg aggccagtgg ttcgactcca ctgcatccca
       61 ccatgaattg agtggaagcc ccgggtttgc ccggggcatt tccctgtacc gggccatgcc
      121 ggcctgccaa ggcgaatctg gctacaaaac gcacataaat gcattatgcc cctgcttggg
      181 catcgtatac gccccttcag cagaacatat actattggct gaagggggca agaatcgatg
      241 gactgtgtcc agatcggctc ctaccttgcc gccgactcgt tgagacggcg gctcttttgt
      301 gagcttgacc atcttcgcgc tcagggcctc gacatatcca ttcgctacag ggttctgggc
      361 gatttgacac taatagaatg caccgccaag tcgtctcgga atacgctagt gaacgatcag
      421 gacctgcggc gtctgtttcg gcgcaggctg gcgaacctgg ctgcaggcat gatctttgac
      481 gaggttgagg aggcgctgat ccttgatacg ctgagaactc gccatcctga gctggaagag
      541 gaagagatcg cagcggtgtc ggagtatgcc gtggccctgc tcaatgaggg tgtgggcggt
      601 gtgttggagc cttcgatgcg gctcagggaa gtcacgacag atgtggaaga gtaccttagc
      661 gactccaatg ccctggtgat tgaggggttc atcaggttcc gcatgaagga gtacatggaa
      721 gagctgaagc ggtctgccga ggaagcgctg atcaggttca tggccgaacg agagcatcgg
      781 gagtttgtgc gtctactgaa atactttgtc gacatacagg accagcgaca ggagactgtt
      841 catgtggtcc ggacagcaga gaaccgtttt gagctgagag actctgaagg cggtcgtgtg
      901 gtcggagact atatggacac tctggcgtcg gacgctcccg gcgagggcgt ggatatgtcg
      961 gatctcttga taagcgcgct gatcacggcg gcccctcgca aggtggtgct gcactttgaa
     1021 cccgactcca ctacggcgga gactatcgat acggtgttcg aaggccgagt ggagaggtgc
     1081 cgcggcaagg actgcagggt cgacgggtgt agaatgcggc atccatcagc tacaggcgag
     1141 gatcccgtaa cgccggcgaa gcgacagtga agctacggat cgggcaggac cagactgccc
     1201 ggctcgctgc ccgacccagc gggaagcccg caaggccggg cagtgttgcc tcgtgcttac
     1261 gcactgatcg gctccgggtc cggcggtcgt ttagcgcttg cgcgccatct gctccactac
     1321 ggccggcagg agtcgccagt tgttgtctac cgactcgggg ccgaacttgc caacttcccg
     1381 cagatagtcc accatgatct cgcggacttc cgtggtagac tccgacaaca actgggcatt
     1441 gcggaacata gtgtacccac cgccgccgcc tgccctatag ttgttgaggg ccaccacgaa
     1501 ctcctggtcg ggccggacat cctcaccctt gaacttgagc gtcacaaccc gctgacctaa
     1561 gggtttggat atgtctacag tgtactcgac gccctggtat atatcgtagt tgtaaccacg
     1621 catcgaggtg ttgatcaggt cgtccaggtt ggtctgcccg gtataggtct tgaagtagtt
     1681 ggcgctggct tcaattgcct cgcgcagttg ctgcccgttg atcttgatgg tgtagagtgt
     1741 gttcgggtat acatataggc catacacgtc ctgcatggtc attggccctt tgttgattct
     1801 cgaggtgtcg gtgaacagag cagcaatgga gatgtcggcg ccggtcgccc acagttgcgc
     1861 ctcattgatc atctcgacca aggcgttgtc gtagaatcta gccgggggtc cgccaggcat
     1921 gtcgcctgtg gcctgcgcga ccgggctctt gaagaagtcg acagtctggt catggtagta
     1981 tttggctgcc tcgagaactt cgggcgcagg ttgcaccccg gtcatgggga caaccgtcga
     2041 ggacctctct gccacgcgcc agcgtccgtc tgcaccgcgc tccagcgcaa tgtcgatccg
     2101 ggatacgtag tcgccccact ggccgggctg ggtgatgatc acatctgtcg ccactatgtc
     2161 gcccatcttt gcgaccatgg ggacggcaac gtggtcgtgg ccggcgacga tggcgtccag
     2221 accgggcacg ttatttgcca gcctgagcgc ccagttctct tcggcctgcc cggtatctac
     2281 ccagttgtcc gggctacccc atgcttcgag cttgccgctg tctttgggtt ttcgttccca
     2341 tcctgtgtga ccgacgacga tcactacatc ggcgccttcc gcgcgcatct ggggtatgta
     2401 cttgagagca gtctccagag gactcatgaa gaccaggcct tctatgttct ccggtttctc
     2461 ccacgtcgga accttaggag tcgtaatccc cagaactccc accttgaact cgccgaaatc
     2521 cttaatcatg tatggctgga tgaacgattc gccgctctcg cgtacgatat tgcctgatat
     2581 cacagggaat ttcgcctcag atatggcctt gcccagagtc ttgagcccgt agttgaactc
     2641 gtggttgcca aggctcatcg aatcgtatcc tatgaggttc atcaccttga tcatgggatt
     2701 gggggcgctg gaatcgaaca tgtagtggta cggcaggacc gaattgcctc cctcaagcac
     2761 atcgccattg tctaccagca gcacgttcgg gctatcactt cgcacctgtt tcacgtaggt
     2821 gtagatctgc gccaggccgc cctcgtctgc ctggttagtg aagtaatcga tcgggtagat
     2881 gtggccgtgc aggtcactgg tcgctagcac caccagacga cttcggtccg tctcggccac
     2941 ggtcgccccg gtgaccccca gtgcaacgaa aagcagcgcc acgactgcag ccagcagcct
     3001 aaggtttcgg gtgggcaaag agcggttgct caaaccgaca tacctccttg tgcgggacgt
     3061 ggtttctggg atgcgacgcc gcgacgcgtg caccagtctt ccacgtgttt ccgcatatat
     3121 aatgggtctt tagtgtggta acttccatac ggataggatc tttccttctc acggccgacc
     3181 aggcattccc tgtagtaccg cgggcatgtt gacaaccccg ccgcacaagg ctatgatggg
     3241 gataatccaa aacagcatgt agcgatgaag aggacgagta ggctgaagcg agcccaaaga
     3301 gacggcgggc atcgggtgta agcacgccgg ggagtcggca gccgaatacc acctccgagc
     3361 ttccggttga aatgtacttg gccggcccag cagcaggtca cgggcggctc aggcagaata
     3421 agccggcacg gctggccacc gttagatggc tccacgagag gacgtccgga tatccgacgt
     3481 caaaccgggt ggaaccgcgg gttgaccccg tcccgatgca tgtgcacgag gcggggtttt
     3541 tgcgtaccta ttgcggccca gttcgtggcg gagagtgccg tttgctattg cggggcggca
     3601 caaggttgta gcacagtcca cgatgagggg gatgtaccag atggagagta tacgcatatc
     3661 gcttcccgac ggatcggtaa ttgaggcgcc gcgcgggacg actatccggg acctggctgc
     3721 tcgtttgagc ccgcggttgg cggcccgggc aa
//
LOCUS       DAOTAC010000254         3719 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_19929_length_3719_cov_5.219159, whole genome shotgun sequence.
ACCESSION   DAOTAC010000254 DAOTAC010000000
VERSION     DAOTAC010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19929_length_3719_cov_5.219159"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="PLM74_09860"
     CDS             <1..485
                     /locus_tag="PLM74_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43414.1"
                     /translation="TRIEVRQRINGAQRPTVEQFARGDWSHHPFGNIMVADEQELQRL
                     HHLTRQAFSRKGLHKGLRDYQRRNGVSKAGRMKKDLLKPFTELMDAWDALRAAWHASA
                     PVPCLVYDTSQRPSTCSSTRPSAFACPYGAEVWAFPSARVSAFDVRPCAVSAELISSG
                     "
     gene            complement(1215..1784)
                     /locus_tag="PLM74_09865"
     CDS             complement(1215..1784)
                     /locus_tag="PLM74_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43415.1"
                     /translation="MAKINEEHIISIDGKPYVKYAGLLEAAHKSGLKDLQVKLIQSPT
                     RDNGYTAVCEATAVIKTRNREKVYIEIGDANPASIGDPRLNVHSIRIAATRAKARALR
                     DALRISICSVEELALGGPQREVSDASASAQTSSQNKATEAQLRVLRIQADKLGLPQED
                     YARFEIMTKREASDAISEFNRQLSRSQAG"
     gene            complement(1855..2460)
                     /gene="radC"
                     /locus_tag="PLM74_09870"
     CDS             complement(1855..2460)
                     /gene="radC"
                     /locus_tag="PLM74_09870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HON43416.1"
                     /translation="MSACYPDFRGLSASQLIAMTLRDNDAASILDRFQNLRAMEEASV
                     EDIAAAGITEEQAATLKAAIELGRRCLTERRSKAYIKSAQDIYNLLGYEMGRLDREQI
                     RVVMLDIRNGVIDTEIVSIGTIDSCVGHPREIFKNAIVKGAASIILTHNHPSGLASPS
                     PEDLALTKRLMEVGKFLGIEVLDHVVIGDGCYCSIKDLMSQ"
     gene            complement(2607..2870)
                     /locus_tag="PLM74_09875"
     CDS             complement(2607..2870)
                     /locus_tag="PLM74_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43417.1"
                     /translation="MKKIANINEALTYVSRLGFTPNGTIHHQQACLYALCEALEQAAG
                     GYPYEVRFQRYVSAQGISFPWLGRFTVKGASGAVVVEAKTVKS"
     gene            complement(2867..>3719)
                     /locus_tag="PLM74_09880"
     CDS             complement(2867..>3719)
                     /locus_tag="PLM74_09880"
                     /inference="COORDINATES: protein motif:HMM:NF019997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ArdC family protein"
                     /protein_id="HON43418.1"
                     /translation="KAAKGCDVIMKTSELMDQIESRIKELATKTEDAKASEEVTQYLR
                     FLSRFHRYSLHNTMAIWIHCPNATHVAGYNAWMKLGRYVRKGERGIPIFAPCTQAVPK
                     GEEEDDEKKTRKVIGYKIVYVFDVSQTEGKPLPQAPIVAQGDGSALLPVLEGIAFDLG
                     IGLEYRAMSGSHHGTSYGGSIDVDSGLDDAGKSSVIIHELAHELLHKGADRMLLSTRQ
                     KETEAESTAYVVCSHFGFDSAAPNYLALWDAKPEQIIEAFQRIRDVAASLIDMIEAKF
                     NAEEVCA"
BASE COUNT          740 a         1106 c         1039 g          834 t
ORIGIN      
        1 acacgcgcat cgaggtgaga cagcgcatca atggcgctca acgtcccacg gtggagcagt
       61 tcgcccgagg ggattggagc catcatccat tcgggaacat aatggtggcg gatgagcagg
      121 agcttcagag gttgcatcac cttactcgcc aggccttcag cagaaagggc ctacataagg
      181 gactgcgtga ttaccagcgc cgcaatggcg tatccaaagc cggtcgcatg aagaaagacc
      241 tgctcaagcc cttcactgag ctcatggacg cctgggatgc cctgcgggct gcatggcacg
      301 ccagtgcgcc cgtaccgtgc ctggtctacg atacgagtca acgaccgtcc acatgctcat
      361 ccacgcgccc ctctgcgttc gcctgcccgt atggtgcgga ggtgtgggcg ttcccttcgg
      421 cacgcgtctc tgcgtttgac gtcaggccct gcgctgtatc ggcagagctc atatcctctg
      481 gatgatgagg caacttcatg ccatggcggg aaagacagac actgctggca atggtcgagc
      541 tgtgtcgatc aggcatgatg gccatgctca attctcatcg cagaatgccc caggaacgtc
      601 gcataggcat gagaggatac aatcacttta ccacctccca cttgtaggct tgtagagcct
      661 tctgccgaaa acacgagccc tctagctggg acgttacgga cacactttca cactaccacc
      721 cccactacgt ggactgcgcc agagtggaca aaggagagaa cgctgaatga ccctacagcc
      781 catgctgtat gaatatacat gcccgcaatg ctcctcgcgc atagtcgcca tcagggccat
      841 ggtgtggtgc aagtgccgca cgaggatgat cagagaggat gaatggaggc gcatacggta
      901 ccaccgcgac aagtccacat gcaagtcatc gagacaggct cctgagacat gagacgcagc
      961 acactatgta ttgcaaggag gcccctggca gactactgat ggagtccagt atggggcctc
     1021 cgctacggag cgacgcccag ttttgaggta aatttcgcga agcgtagtca tcgcttcgat
     1081 gtgtgtttcg gtgtctggag caggtattat tatagtagag ggacgttctg cgagggatgc
     1141 cgacttgtca ctgcccgacg ggataaaaca ggggcccgtc agggccccca cgaacgtacc
     1201 gggcttatcg gcggctaacc ggcctgagac ctgctgagct ggcgattgaa ttcgctaatc
     1261 gcgtccgagg cctccctctt ggtcatgatc tcgaagcggg catagtcctc ttgaggtagc
     1321 ccgagcttat cggcctgaat tctaagtact cgaagctgtg cttccgtcgc cttgttctga
     1381 gatgaggtct gtgcagaggc agacgcgtcc gacacctccc gctgaggccc tccaagcgcc
     1441 agctcttcca cggagcaaat ggatatgcgc agcgcgtcac gcaaagcccg tgctttggcc
     1501 ctggtcgccg ctattcgtat cgaatgtacg ttcaaccttg ggtctcctat cgatgccggg
     1561 tttgcatcgc cgatctcgat atagaccttc tcccgattcc tcgtcttgat gactgctgtc
     1621 gcctcgcata cggcggtgta cccgttatcc ctcgtgggag actggatgag cttgacctgc
     1681 aagtccttga gcccagattt gtgggccgcc tccaataggc ccgcgtactt cacgtagggc
     1741 tttccgtcaa tgctgatgat gtgctcttcg ttgatcttcg ccattgttat ttctcctttc
     1801 gttcatgggg aatgaatgcg ggcgccagct gcagcgccag cgcccggcag cgcgctattg
     1861 ggacatgagg tccttgatgg agcagtagca gccgtcaccg atgaccacgt ggtcgagaac
     1921 ctcgatgccg agaaactttc caacttccat tagccgcttc gtgagggcga gatcctcggg
     1981 ggaaggtgat gccagccctg aaggatggtt atgggtgagg atgatggatg cggcgccctt
     2041 cacgatcgcg ttcttgaaaa tctctcgagg atgtcccacg cacgagtcta tggtgccaat
     2101 ggacacgatc tcggtatcga tcacgccatt gcggatatcg agcatgacca ccctgatctg
     2161 ttccctatcc aggcggccca tctcataccc aagcaggttg tagatgtcct gtgccgactt
     2221 gatgtaggct ttgctccgac gctcagtcag gcaccgcctg ccaagctcga tggcggcctt
     2281 gagcgttgct gcctgctctt cagtgatgcc cgctgcggcg atgtcctcta ccgatgcctc
     2341 ttccatcgcc ctgaggttct ggaagcgatc caggatgctg gcggcatcgt tgtcacggag
     2401 cgtcatcgca atgagctggg acgcgctcag accgcggaaa tcgggatagc atgcactcat
     2461 gaatcaatct cctcctcgca tgattttggg aaagcagagg aggacggcag ccgctctcgc
     2521 tcgcggcgcc gccctccgat acacccgatt ctgacaataa gaaaagcccc actatggggc
     2581 catcggggat atgcgtgttg tccttgctag ctcttcacgg tcttggcctc gaccaccact
     2641 gctccgctgg cgcccttcac ggtgaaccgc ccgagccagg ggaagctgat gccctgggcg
     2701 ctcacatacc tttggaatct gacctcgtaa gggtatccgc ctgctgcctg ctccagagct
     2761 tcacacagag catacaagca ggcctgctgg tgatgaatag tgccgttcgg agtgaaccca
     2821 agcctgctaa cgtacgtcaa ggcctcattg atgttggcga tcttcttcat gcacacacct
     2881 cctctgcatt gaacttggct tcgatcatgt ctatcaagga cgccgccacg tccctgattc
     2941 gctggaaggc ctcaatgatc tgctcgggtt tcgcgtccca caaggccagg tagttcggag
     3001 cagcgctgtc gaacccgaag tgcgagcaca caacgtaggc cgtagattcg gcctcggttt
     3061 ccttctggcg ggtgctgaga agcatgcggt cggctccttt gtgaagaagc tcgtgtgcca
     3121 actcgtggat gatcacagag gacttgcctg catcgtcaag ccccgagtcc acgtcgatgc
     3181 tccccccata gctggtgcca tggtgagatc cggacatggc ccgatactcc aggccgatac
     3241 ccagatcgaa tgcgatgccc tcgagcaccg gcaggagcgc actgccatcc ccctgcgcca
     3301 ctataggcgc ttgcggcagg ggcttgcctt cggtctggct cacatcgaac acatatacta
     3361 tcttgtagcc gatcaccttg cgagtcttct tctcgtcatc ctcttcctcg cccttgggaa
     3421 ctgcctgcgt gcagggcgca aagattggaa tgccccgttc gcccttgcgc acgtatcttc
     3481 ccagcttcat ccacgcgttg tagccagcca cgtgcgttgc attggggcaa tgaatccaga
     3541 tggccattgt gttgtgcagg ctatacctgt ggaacctgga cagaaacctg agatactgcg
     3601 tcacctcctc ggaggccttg gcgtcttcag tcttggtcgc gagctccttg atcctggact
     3661 cgatctggtc catgagctca ctagtcttca tgattacgtc acatcctttc gccgctttt
//
LOCUS       DAOTAC010000255         3670 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_20257_length_3670_cov_3.349654, whole genome shotgun sequence.
ACCESSION   DAOTAC010000255 DAOTAC010000000
VERSION     DAOTAC010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20257_length_3670_cov_3.349654"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1184
                     /locus_tag="PLM74_09885"
     CDS             <1..1184
                     /locus_tag="PLM74_09885"
                     /inference="COORDINATES: protein motif:HMM:NF014047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HON43419.1"
                     /translation="KVEDIVVAAVGPREDVERKALPVVTGDLIDVVIEEPHMTNSRDG
                     IARLEGYVIDVEGAGRMIGARVKAKVTKVFKTYARARLVSADGSLSQEEQPQEPAPGD
                     QVSAAKAQEPAGTNEIAAASALPISTPAPAAARVAAPASAPAPASPTASVSDSGAAAV
                     SAQLRPQNQEKNKASQKAQPTQPAQQGRAQGKANDKAKQQAKPEQEGKAVGQKQGARQ
                     SAEEQQPAPDTNTTVDKQAAAPSSTRGAKRNGRRSRRGSGRAKAGDQPAKPSPAPVGG
                     QAAAPPAEQAVAQPIPVTVDAVAAAGTATSIPPVAPSAVPAATGSADPSSPQRPSSRS
                     SRRGGRQARAARARKAARAAKAAASGVADAAAAAADAGAGARQTDAAGRVQNVAEQET
                     V"
     gene            1558..1872
                     /gene="rplU"
                     /locus_tag="PLM74_09890"
     CDS             1558..1872
                     /gene="rplU"
                     /locus_tag="PLM74_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HON43420.1"
                     /translation="MYAIVETGGKQYRVSPGQVVRVEKLSTALGDTVEFDRVLMVVGD
                     DGVRVGQPVVNGAKVTGAVVAHDKAKKIYVFKYKAKKRIRKKTGHRQPFTSVRIDSIV
                     TE"
     gene            1886..2254
                     /locus_tag="PLM74_09895"
     CDS             1886..2254
                     /locus_tag="PLM74_09895"
                     /inference="COORDINATES: protein motif:HMM:NF016233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HON43421.1"
                     /translation="MTTVTVWRSGGHVVRFTVRGHSGWAEYGHDIVCSAVSALTTAAA
                     NGLRQQAGVCRAVRQRHGELDCMILGSPSDSEWNRADAILETMFAGVCSIAREYPGTV
                     LVVERNRPESGRDLQARGCN"
     gene            2254..2583
                     /gene="rpmA"
                     /locus_tag="PLM74_09900"
     CDS             2254..2583
                     /gene="rpmA"
                     /locus_tag="PLM74_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HON43422.1"
                     /translation="MIRMDLQLFAHKKGMGSSRNGRDSQAQRLGIKKFGGQRVTAGSI
                     IVRQHGTKVYAGVNVGMGKDYTLFATADGLVQFDRFGKTGKRVSVVPDSEAAVAGLEV
                     APAAETM"
     gene            2675..>3670
                     /locus_tag="PLM74_09905"
     CDS             2675..>3670
                     /locus_tag="PLM74_09905"
                     /inference="COORDINATES: protein motif:HMM:NF015644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HON43423.1"
                     /translation="MSRASGWVKALIVVLLGVALAWNTIAVIVTEHLWYADLGYLGVF
                     WKRAEARVIVCVAAFAVTFAFVAINLLATRAASRLVSTRAALMIAAASGLAAAAASYG
                     SWMEFQQFVHAASFGKADPIFGRDISFYVFRLPIIRQIHAGSRWVVGLTAASVILIYL
                     VSGALVRFGREQVEPGTPGGAAFGRRKRGRIVLEPIDARAKLHVCVLLGIGLCIVALG
                     FALSMWGLIYSTRGVVTGASYADVHGTLPGLRLLVWLMLASAVFIVGTGLRAASKRTW
                     AVVCITFIVLLGVSFFAIDVYPSIVQKLYVTPNELVAEREYIGYNIDFTRSAFGLD"
BASE COUNT          728 a         1021 c         1210 g          711 t
ORIGIN      
        1 agaaggtcga ggacatcgtt gtggcggctg tgggccccag ggaggacgtg gagaggaaag
       61 ccctaccggt ggtgacgggt gacctgattg acgtggtgat agaggaaccg cacatgacca
      121 acagccgcga cggaatagcg cgactcgagg ggtacgtcat cgacgtggaa ggcgccggcc
      181 gtatgatcgg ggcgcgtgtg aaggcaaagg tcacgaaggt cttcaagacc tatgcccgcg
      241 cgcggctagt ctccgccgac ggaagtctct ctcaggagga gcaaccccag gagcccgcgc
      301 caggcgacca ggtctctgct gcgaaggccc aggaaccagc gggcacgaat gagatcgccg
      361 cagccagcgc tcttcccatc tcgaccccag ccccggctgc agcccgtgtt gccgctcctg
      421 cgtctgctcc tgcgcccgcg tctcctactg cttctgtttc cgactccgga gcggctgctg
      481 tttccgctca gcttcggcca cagaaccagg agaagaacaa ggcttctcag aaggcacagc
      541 cgacgcagcc ggcgcagcag ggccgggcac agggcaaggc caacgacaag gcgaaacagc
      601 aggccaagcc ggaacaggaa ggaaaggccg tcggtcagaa gcagggcgcc aggcaatcag
      661 cagaagaaca gcaacccgcg cctgacacga acaccactgt tgacaagcag gcagcggcgc
      721 caagctcgac ccgcggcgcc aagcgcaacg gtcgcaggtc aaggcggggt tcgggccgtg
      781 cgaaggcggg cgatcagcct gcaaagccat cgcctgcgcc ggtcggaggc caggctgccg
      841 caccgcctgc ggaacaggcc gttgcccagc cgattcccgt cacggtcgat gcagtggccg
      901 cggcaggcac agcaacctcg attcctccag ttgctccgag cgcagttccg gccgcaaccg
      961 gaagtgcaga cccgtcttcg cctcaaaggc cttcgtcccg ttcatctagg aggggcggcc
     1021 gtcaggcgag ggcagccaga gccagaaagg cagcgagagc cgccaaagca gctgcatccg
     1081 gcgttgccga tgccgctgcc gccgctgctg atgctggtgc tggtgctcgg cagactgacg
     1141 cagccggacg cgtccagaat gtagccgagc aggagacagt gtaggcaacg tagcctttca
     1201 caccggccga taggcccgca tggcgcaaac ggacgggggc cacaccgact gcccaacata
     1261 gcaggcagtc agctggcccc cgacagattt ggccggctga gcagggatga ttatgatagc
     1321 gcgaggccaa aacggctgga gctggcatat cgtcaattag cgggtgtgtc gtgcgtgagt
     1381 tgcggggtga gtttgacacc tatccatgct tgtgctatat tttaaccgct gtgggcgacc
     1441 ttgtacgtcg cctcaagccg ctcgggaaag gcctgtaaat ggagcgcggg agggattgca
     1501 ccgcgtttcc tgcctttgat ggtcccggcg agcccagagt gcgaggaggt cagataattg
     1561 tacgcaatcg ttgaaaccgg cgggaagcaa tatcgtgtat cgccgggcca ggtcgtccgc
     1621 gttgagaagc ttagcactgc tctcggcgac acagtcgagt ttgacagggt gcttatggtg
     1681 gtcggcgatg acggtgtccg ggtagggcag cccgtcgtga atggggccaa agtcaccgga
     1741 gcagtcgtgg cccatgacaa ggccaagaag atctacgtct tcaagtacaa ggcgaagaaa
     1801 cgcatacgca agaagaccgg ccataggcag ccgtttacgt ctgtcagaat cgactcgatc
     1861 gtcactgagt gaagatacgc ctgatatgac tactgtcacg gtttggcgat ccggtggcca
     1921 cgtggtgcgg ttcacagtgc ggggtcactc aggttgggcg gaatacgggc acgacatagt
     1981 ctgttccgca gtatccgctc tcaccactgc tgcggccaac gggctccggc agcaggctgg
     2041 tgtgtgcaga gccgttaggc agcgccatgg cgaacttgat tgcatgatac tgggttctcc
     2101 gtccgactcg gagtggaacc gggcggatgc gattctcgaa acgatgttcg caggggtttg
     2161 ctcgattgcg cgggaatacc caggcaccgt attggttgtg gagagaaaca ggcccgaaag
     2221 cggccgcgat ttgcaggcta gggggtgtaa ctaatgatca gaatggatct tcagctgttt
     2281 gcgcacaaga agggaatggg aagctccagg aacgggcggg attcccaggc gcagaggcta
     2341 ggcataaaga agtttggcgg gcagcgtgtg acggccggca gcattatcgt acggcagcat
     2401 ggaactaaag tgtacgccgg agtcaatgta ggcatgggta aggactacac tctgttcgca
     2461 accgccgatg gattggttca gttcgatcgg ttcggcaaga ccggcaaacg ggtctcggtc
     2521 gtgccggact ctgaagcggc ggtagccggt ctggaggttg ctccagccgc cgaaacgatg
     2581 tagtgagcaa cggggatatg tcggagcccg catcagcttt gcttggtgtg ggttccttcg
     2641 tttttgtgac aatcctcttg aaggggtgga actaatgagc agggcttccg gatgggtcaa
     2701 ggccctcatt gtcgttcttc tcggcgtggc cttggcgtgg aacactattg cagtcatcgt
     2761 gacggagcat ctgtggtacg ccgacctcgg ctacctcggc gtgttctgga aacgtgcgga
     2821 agcgcgtgtc atcgtgtgcg ttgcggcatt tgctgtcacg tttgcctttg tggcgatcaa
     2881 ccttctggct accagagccg catccaggct agtctcaacc cgggcagccc tcatgatcgc
     2941 cgcggcttcc gggctcgcag ccgcagcggc gtcctatggc tcgtggatgg agttccagca
     3001 gtttgtccac gctgcgtcct tcggaaaggc agatccgatt ttcggccgcg acatcagctt
     3061 ctatgtattc agattgccga taattcggca aatccacgca ggttccaggt gggtagtggg
     3121 gctcacagcc gcatctgtca tactgatcta tcttgtgtcc ggagcattgg tcaggttcgg
     3181 acgcgagcag gtggagccgg gcaccccggg cggcgcagcc tttgggaggc gaaagcgcgg
     3241 caggatagtg ctggaaccca tcgatgcccg ggccaagcta catgtgtgcg tgctgctcgg
     3301 cataggcctg tgcatcgttg cgcttggatt cgcgctctcc atgtgggggc ttatctactc
     3361 taccaggggc gtagttacgg gcgccagtta cgcagatgtg catggcaccc tccctggtct
     3421 gcgtctgctc gtctggctaa tgcttgcgtc cgcggtgttc atcgtaggca cgggtcttag
     3481 ggctgcgagc aagcgcacgt gggcggtggt gtgcattacc ttcatcgtgc ttttgggagt
     3541 gtcgttcttt gcgattgacg tttaccccag cattgttcag aagttgtatg tcacacccaa
     3601 tgagcttgtt gcagagcgcg aatacatagg gtacaacatt gatttcactc ggagcgcctt
     3661 cgggctggat
//
LOCUS       DAOTAC010000256         3646 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_20440_length_3646_cov_5.534113, whole genome shotgun sequence.
ACCESSION   DAOTAC010000256 DAOTAC010000000
VERSION     DAOTAC010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3646
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20440_length_3646_cov_5.534113"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="PLM74_09910"
     CDS             <1..220
                     /locus_tag="PLM74_09910"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HON43424.1"
                     /translation="KHPGTASEANHSVRRRCEICGKLIPKERTEALPDTKRCIECARS
                     RGTDVSAKRADVGMDPDTYKDLLGAVRS"
     gene            263..618
                     /gene="rnpB"
                     /locus_tag="PLM74_09915"
     ncRNA           263..618
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLM74_09915"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            850..1008
                     /locus_tag="PLM74_09920"
     CDS             850..1008
                     /locus_tag="PLM74_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HON43425.1"
                     /translation="MSKWVCTVCGYIYDPEVGDTDGGIAAGTAFEDIPDDWVCPECGV
                     GKDAFEEA"
     gene            1109..1606
                     /locus_tag="PLM74_09925"
     CDS             1109..1606
                     /locus_tag="PLM74_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HON43426.1"
                     /translation="MAAFAGESQARNKYDYFASAAKKEGFEQIAAIFEETALNEKEHA
                     KRLAKFVGLVGATDANLKHAAEGENYEWTSMYPEFERVAREEGFGDIADVFREIGEVE
                     EHHEARFRALLTNIENGTVFKKDKPVSWKCRNCGYIHEGPEAPKVCPACAHPQSYYEL
                     NVKNY"
     gene            1648..1983
                     /locus_tag="PLM74_09930"
     CDS             1648..1983
                     /locus_tag="PLM74_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HON43427.1"
                     /translation="MKLWRCGVCGYIHDGDSPPERCPKCGAPKEKFSALDEAEANLVH
                     RSRFTNDLHCKLISLMDEVRKIAEAGIEDDLDPPCVTLFKHALEQAVVIGQSSKAEIR
                     GHMGKGKWG"
     gene            2607..3139
                     /gene="spoVT"
                     /locus_tag="PLM74_09935"
                     /pseudo
     CDS             2607..3139
                     /gene="spoVT"
                     /locus_tag="PLM74_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412147.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
     gene            3361..3564
                     /locus_tag="PLM74_09940"
     CDS             3361..3564
                     /locus_tag="PLM74_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43428.1"
                     /translation="MKVTNVRGVLVATIALDPGDSGEISGLSDAALVMRARRAVSTVI
                     PDYLIRDILVGRDDDVLEVAFTV"
BASE COUNT          852 a          891 c         1213 g          690 t
ORIGIN      
        1 caaacatcct ggtaccgcat cggaggcgaa tcacagcgtg aggcgcaggt gtgaaatctg
       61 tggtaagcta atacccaaag agaggactga ggcgcttccg gacaccaagc gctgcatcga
      121 gtgcgctaga agcaggggta ccgatgtatc ggccaagcgt gctgatgtgg gtatggatcc
      181 ggatacgtac aaagacctcc tcggggccgt gcgcagttga cgccatggtc ggcaaggtgt
      241 tagcatgtgt cgtgaagagt gacaagcagt ctgagtgatc gcgggcgagc gagcgttcgc
      301 ctgaggaaag tccgagctcc gcagggcagg gtgctgggta acgcccagtg aaggcgactt
      361 caaggaaagt gccacagaaa tcaaaccgcc atgcgcagcg cgtttggcgc atggtaagag
      421 tgaaacggtg cggtaagagc gcaccagcgt ccgggcgacc gggcggctag gtaaacccca
      481 cccggagcaa ggccaaatag gagggatatg aagctgcccg cttctccctc gggttgggcc
      541 gcttgaggct cgtggtaaca tgagtcccag atagatgatc actctcgaca gaactcggct
      601 tacagggctg cttgttgcgt ttgtgatgcc cgggctcgtc agacgacggc tccgggcatc
      661 tccctttttt ggggtgttcg ctgggcgagg ccggcacagg cgcactcgcg ggggccatga
      721 cgggcgtcga gtacccagcg ggaacactta tagcgggaac atgttgacag gtggcatcgt
      781 tgcgtgttat agggatgtac cggaggaatt tggagcggta gtgcattact tctttggagg
      841 tgtaacgtaa tgagcaagtg ggtctgcacc gtatgcggct atatctacga ccccgaggtg
      901 ggcgacacgg atgggggaat cgccgcgggc actgcctttg aggatatacc cgacgattgg
      961 gtatgcccgg agtgcggtgt tgggaaggac gcgttcgagg aggcgtaggc gcaaacgccg
     1021 agcatgaagc aacgcgcttg gacgaaatgg aaggaggttg acggttttgc gcaaagatct
     1081 cgaaggaacc aagacatatg agaacatcat ggctgccttt gcaggagagt cgcaagcgag
     1141 gaacaagtat gactactttg cctcagcagc caagaaagag gggttcgagc agatagcggc
     1201 catcttcgag gagacagcac tgaacgagaa ggagcacgcg aagaggttgg ccaagttcgt
     1261 gggccttgtc ggagcgacag atgcaaacct gaaacacgct gcggaaggcg agaactacga
     1321 atggaccagc atgtaccctg agttcgagcg tgtagcccgt gaagaggggt tcggagatat
     1381 cgccgacgta ttccgggaga tcggcgaggt cgaagagcat cacgaggccc gtttcagagc
     1441 tctccttacc aatatcgaga acggcactgt gttcaagaag gacaagcctg tcagctggaa
     1501 gtgccgcaac tgcgggtaca tccacgaggg ccccgaggca cccaaggtgt gtcctgcatg
     1561 cgcccatccc caatcatact atgagctcaa cgtgaagaac tactaaggct cgcctggggc
     1621 aatcccgcga cttatggagg tgtaagcatg aagctgtggc gatgcggagt gtgcgggtac
     1681 atacatgatg gcgatagtcc gccggaaagg tgccccaagt gcggcgctcc caaggagaaa
     1741 ttcagcgcgc tggacgaggc cgaagcgaat ctggttcaca gatctcgctt caccaacgat
     1801 ctgcactgca agctgatatc cctcatggat gaggtgagga agatagcgga ggccgggatc
     1861 gaagacgatc tggatccgcc atgtgtcact ctgttcaagc acgctttgga gcaggccgtg
     1921 gttattggcc agtcatcaaa ggctgagatc agagggcaca tgggcaaggg caagtggggc
     1981 tagagagagg ctgtcggtcg cgagggcata gctgaagatt tgcaaatcga ccacaatgtg
     2041 ccatttaccg gacgcatatt tggccgatct cgggccatca tacataacga ggtttgcgac
     2101 ggtcgagcga agatctgcgg cggacttgac catagcaatg atagtcgagt ccgctgtggg
     2161 tcgagcaaag gtcgactgtg gttccaaagg tgatcacagt cggccggcgc gaagatcgtt
     2221 gcaggtggcc caacttatgg cggcggttga tgagatcgtc gctgtaggga aatctatctg
     2281 cagcggtcga gctaagatca gcgtacaagc gaaagcgtgg tgctgcagtc gagagattgc
     2341 agtatgatgc ccaagtttgg acgttggtcg atcaaaggtt gttccactcc gtgtaatggc
     2401 cgctcggcgc cgaaaggggt cgggtagtca cgcaacgggg tgtagcagcc ggagagcaga
     2461 tcgccgcagg cctccttttt gcgtacggaa taggcaccgc ccttgcgagg tccgcatgcc
     2521 cgtccccgcg gccgatccgc gggagcacgc acaagagcat aagctagttt cggcaaaggt
     2581 agaatactag caagggaggg atgcgggtga aggcgacagg cattgtccgc agaatcgacg
     2641 acctagggag ggtcgtgatt ccgaaagaga tccgaagaac ccttcgaata cgcgagggtg
     2701 atccgcttga gatattcact gaccgcgaag gcgaagtgat cctgaagaaa tactcgccca
     2761 ttgctgacct gggtgatttc gcgcaggaat acgccgactc gctccacgac ggcactggtc
     2821 atgtcgtact gattgctgac caagacgaga ttgtggcggt ctcgggggcg cagaagaagg
     2881 gtttcgttga gcggcgtatc acggaacacg ttgaggaggt aatgcgcgct cgcaaaccgt
     2941 tcctgtcgaa tgtccccaat gagagccccc catacaggat ctggagttca caagccaggt
     3001 gatggcgccg atcattgtgg aaggggatat tgcgggcgca gtcatactcg tctcgaaaga
     3061 ggaccgcacc atgggcgacc ttgagctgaa gctggccgag accgcggcag ggttccttgc
     3121 aaagcaactc agtcagtagg ttacttgcag cgatgagggc cggcctcgcg atgtttcggg
     3181 ctggcccgtt tgatttcccg ttttgcggga aacgccgata ggacagaacg cttgaccgag
     3241 gcaaacggca acagtcgtag cgcatcctgc gaaactgatc gaaaacccat cgggacgtgt
     3301 gcggaggaca cgccgctctc gcgtcgaaac gagtgctaga cgcgggagga ggccgatccg
     3361 atgaaggtta ccaacgtcag aggagtgctt gtcgccacga ttgcactgga cccgggagac
     3421 tctggagaga tctccggctt gtccgatgcc gcattggtta tgagagccag gcgggcggtg
     3481 tcgacagtca ttcctgacta cctgattcgc gacattctgg tggggcggga cgacgacgta
     3541 cttgaggtgg ccttcacggt gtgaagagcc cggtatggta agtggtgcgc agatagcgta
     3601 atcttagtgt aggctgctgg gtagggaaaa gcggagtaca gcaaga
//
LOCUS       DAOTAC010000257         3606 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_20689_length_3606_cov_4.908195, whole genome shotgun sequence.
ACCESSION   DAOTAC010000257 DAOTAC010000000
VERSION     DAOTAC010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20689_length_3606_cov_4.908195"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(594..690)
                     /locus_tag="PLM74_09945"
     tRNA            complement(594..690)
                     /locus_tag="PLM74_09945"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(654..656),aa:Sec,seq:tca)
     gene            complement(1175..2401)
                     /locus_tag="PLM74_09950"
     CDS             complement(1175..2401)
                     /locus_tag="PLM74_09950"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HON43429.1"
                     /translation="MDSAKESAGRVLALFPMLVFLASMAAIGVISALVLGPTSTSDIP
                     CAIPCATPCATPYVNPSPYDELVYHRKYSEGPNVSARAAILVEASTGTILYARNEHEI
                     REPASITKIMTAIVAIERGDLADIVQVSANAARVGGSSLGLAAGQRYKLEELIRATML
                     RSGNDGATAVAEHVGGSVAGFVELMNARAYSMGLKHTRFANPHGLSAPGHHSTAYDIA
                     LMCIWGFRLRKFADIVGCPELWSSPLAGGQPRLTYSTNRLLWSLAGADGVKTGTTSKA
                     GHCLAASATRDGLQFIAVVLRSGARFHDAAALLEYGFANFVRVDVARAGEPVADGLAG
                     RRGATTAGLATARDVSMVVRRSEVDQLDSQVIVAERLKANTRAGNHAGWLRVLFDEEE
                     VGAYPLYTVESPKKRG"
     gene            complement(2554..3003)
                     /locus_tag="PLM74_09955"
     CDS             complement(2554..3003)
                     /locus_tag="PLM74_09955"
                     /inference="COORDINATES: protein motif:HMM:NF020939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RQC domain-containing protein"
                     /protein_id="HON43430.1"
                     /translation="MDANAPNSVALLEEEELAQIVLECVGSIPWHVGKTKIARILKGS
                     LAQDILKMDLDRIERYGALQDLTLAQITAIIDQLIAGGYLRSSRGRRPIILLTPMGRT
                     ALITGELPGIEVPDALKMFIARERCRKCPYNPSLIASGEGSPAASPW"
     gene            complement(3091..>3606)
                     /locus_tag="PLM74_09960"
     CDS             complement(3091..>3606)
                     /locus_tag="PLM74_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43431.1"
                     /translation="VVQSDGSLLFQSADNIRQVSVASVAGAHMGEAQTVDVRRSELEL
                     PYNLAVPTATLAVRVSGSHVLMSLSSDEPVAWTAFELEGPSGMEVASSEQSYTVEKGR
                     DTVKIRTVLAGESGRFAAGLELSAPSGTQVTVRATGGYRQNPSEIVLTGTGKRFYQGN
                     RFTVPAQSITI"
BASE COUNT          745 a         1108 c         1024 g          729 t
ORIGIN      
        1 tatccatatt atgttacccg cctatgcctg cggatttgtt gccggcgtga ctccgtaaga
       61 agggggcgcc gttgccgacg cgaattcaga taactcgtac ccgccaaacc acccagaatc
      121 cgaggaggtt gatcaaaaag cctgccggaa aacgaatagg atgagccaga atctggttga
      181 gccaaatgcg cctgtatcgg ggaccaggta atcccactct acggcgaata ggatgccccg
      241 gaaccctcgc tagagcccac acaggagaac gccgtaccca gaaatgtcca tggatgtggg
      301 taatgcatag cggccacacg acaggccgag attcgggcct tggaaaccga tagtcccagg
      361 ccggtaagag gcccgccgcc gttgcctaca atccctacac gcacccgact gatggcgagc
      421 taccctaaat cgagctcgaa atcatctgca tatgaagact tgatctcaaa cggcgtaaac
      481 gagatgtgca cgcagacgtg gaccgggaac cggtatggtg cggctcaagc ggcatgttcg
      541 tacagaactc aggatcagcg cccttgtagc tcgcctcgct gaggggagag tgttggcggg
      601 agtacgtggg aatcgaaccc accgcggcca ggttagctgc ccgccactgg atttgaagtc
      661 caggaggccc accaggacca atctactccc gcgtcttcag gctgcacttg cctagacatg
      721 aacccattat agcgcagtcg tcgctgccag tcgagggcca cttcatgcct ctagctccgg
      781 tcgaagcttc tggcaggaag ctcgccgcgc ttctcgccat ttgagcgcgc gagcgcggcg
      841 agcctctctt cagctggaat ccgcaggccg gcaagccctg cctatgggca accaatggga
      901 aaccaatgcg cgaatcaaca cctcgcgctc gatagtagac aacatgcatg cgccattatg
      961 cgccatacgc tgcatgtttg cagttaaacg cctgtctgcc tggccttata tgatctgctc
     1021 gcttttcgaa caggttttcg ccaatgtgtt ccgaaacgcg acacctagct tttcccatcg
     1081 gccgtgccgt tcaccagtgc gcttatgcta accccaggct tggcttcgtt catgaccgaa
     1141 accgcgcgtc cccatggaga tgagttcatt tcgcctaacc ccgtttcttc gggctctcca
     1201 cggtatagag cgggtacgcc cccacctcct cttcgtcgaa gagcactctg agccaaccgg
     1261 cgtgattacc ggcgcgggtg ttcgccttga ggcgctcggc cactatcacc tgggagtcta
     1321 gttggtccac ttcgctgcgt ctcaccacca tcgacacgtc gcgtgcggtg gcgagccccg
     1381 cggttgtcgc cccacgccgc cccgcaagac cgtctgccac cggctcgccc gctctagcca
     1441 catcaacccg tacgaaattg gcaaacccat attccagaag ggccgctgca tcgtgaaacc
     1501 gcgcccccga gcggagcacg acggcgatga attgcagtcc atcccgggtt gctgacgctg
     1561 ccagacaatg cccggcctta cttgtggttc cggtcttgac accgtcggct ccagcgaggc
     1621 tccagagcag cctgttggtg ctataggtca gcctaggctg gccacctgcc agtggcgacg
     1681 accacagctc cgggcagccc acaatatcag cgaatttgcg gaggcgaaaa ccccagatac
     1741 acatcagcgc gatgtcgtat gcggtggagt ggtgccccgg ggcggacagg ccgtgcgggt
     1801 tggcaaagcg ggtatgcttc aggcccatgc tgtaggcgcg cgcattcatc agctctacga
     1861 aacctgccac cgacccaccc acatgctctg ccacagcggt tgcgccatca tttccggacc
     1921 gaagcattgt ggctcgtatc agctcttcga gcttgtagcg ctgtcccgcc gccaacccca
     1981 gggatgatcc gcctacccga gcggcgtttg cagacacctg aactatgtcg gccaggtcgc
     2041 cgcgctcgat agccactatg gcggtcatga tcttggtgat gctggcaggc tctctgatct
     2101 cgtgttcgtt gcgggcgtag aggattgtgc cggtggacgc ctccaccaga atggcggcac
     2161 gcgcgctcac attcggcccc tcggaatact tcctgtggta gacaagttca tcgtatggag
     2221 acgggttcac gtagggggtc gcacatgggg tcgcgcaggg gatcgcgcag gggatatcgc
     2281 tggtcgaagt cggacccaag acaagggccg aaataacccc tatggcggcc atagaggcaa
     2341 gaaagaccag cattgggaag agcgccagga ccctgccggc gctctctttt gcggaatcca
     2401 aggcttgatt cctccggtgc acccacggta caccccccga tgcaccccca ctcgcgtggg
     2461 gctatcgcct cggagcaagg gcgttctcag ctccattcat actcttgcaa acgaataact
     2521 agaacgaccg gatgtcgtag gcacagacct cgactaccac ggcgaagctg cgggcgaacc
     2581 ttcgccggag gcaattaggg acgggttgta tgggcacttc cggcaccgct ctcgcgcgat
     2641 gaacatcttg agtgcatcag gtacctcgat accgggaagt tcgccggtga tgagagccgt
     2701 gcgacccatt ggcgtaagga ggatgatggg ccgtctgccc cgggacgatc gaagatagcc
     2761 gccggcgata agctggtcaa tgatggcagt gatctgggcc aaggtaaggt cttgaagagc
     2821 gccatagcgt tcgatgcggt cgaggtccat cttgagaatg tcctgcgcga gggagccttt
     2881 gagtatgcgc gctattttcg tttttccgac atgccacggg atcgagccca cacactcgag
     2941 cactatctgg gcaagttcct cttcttcgag cagcgcaacg gaatttggcg cgttggcatc
     3001 caattggatg acctcctccc agcaatagta tcatacacag attaccattg ctgtcgccag
     3061 ttgtaaagta gtattttgta attctgcggt ttatatggta atgctctggg caggcactgt
     3121 gaaccggttt ccctggtaga accgcttacc ggtgcccgtg aggacgattt ccgacggatt
     3181 ctgacggtat cctcctgtag cgcgaacagt gacttgcgtc ccagatggcg cagaaagttc
     3241 caggcccgct gcaaaccgac cggattcgcc ggccagtaca gtgcgtatct tcactgtatc
     3301 gcgccccttc tccacagtgt agctctgttc ggatgatgcc acctccatcc ctgacggtcc
     3361 ttccagttca aaggctgtcc aagccacggg ctcgtcagat gacagcgaca tcaagacgtg
     3421 cgatcccgat actcggaccg ccagtgtagc ggtgggcaca gccagattgt atggcagctc
     3481 taattcggaa cgcctgacat ccaccgtctg cgcttcgccc atgtgcgcac ccgccacgga
     3541 cgccacagac acctggcgta tgttgtcggc gctttggaac aggagtgagc cgtcgctctg
     3601 caccac
//
LOCUS       DAOTAC010000258         3605 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_20695_length_3605_cov_4.715211, whole genome shotgun sequence.
ACCESSION   DAOTAC010000258 DAOTAC010000000
VERSION     DAOTAC010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20695_length_3605_cov_4.715211"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="PLM74_09965"
     CDS             <1..741
                     /locus_tag="PLM74_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HON43432.1"
                     /translation="MDKVTREQGFSCRGSDVSCERQKSLMVEKIAREIRAVSKRNGKV
                     LVVAGPAVIHTGAGKHLAALIDAGYVNVFFAGNAVAVHDVESALHGTSLGVYLKDGTS
                     APMGHMHHLLAINKIRDLGGLRQAVEAGVLREGVMHALVKNNVPFVLAGSIRDDGPLP
                     DVITDSVEAQEAMRKGIKGVEIALMMSTMLHSIATGNLLPARVKTICVDINPATVTKL
                     ADRGSHQAVGIVSDVEWFLKELRSYLVA"
     gene            complement(838..2622)
                     /locus_tag="PLM74_09970"
     CDS             complement(838..2622)
                     /locus_tag="PLM74_09970"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HON43433.1"
                     /translation="MLALSVRDLAKAFGARTLFRDVSLEIEAGEKVALFGPNGCGKST
                     LLNIISGRTDPDSGTVWNRPGAILGYLPQSLQFQGDLTPSDLCRDSAVLGRFGIEPAL
                     ASMPMSTLSAGQQRRVGLAQVWAAQPDLIMLDEPTNHLDAPAMDLLARLIRESRAAVL
                     LVSHDRYFLDHTARRVIDLTPRGARSYTGNYTAFRARKDSELEAEWRLYEKQEREEDR
                     LKQVASREMEWFRQAHRRAGQNDFLRARANKGAARARASFARLERFRQQRIAKPREAD
                     AIVLRMGQAQKSGRRLIHAKGISKSFDRPLFGAADVAIMRGDRVAIIGPNASGKTTLL
                     RMLLGLEPSTSGTVWRSPSASWFYFDQTMEQLDPSHTVFESVSSAASTRSPQEVRDIL
                     GALLFRGSAVGKRVSVLSLGEKVRVIFGRMIARGYDILALDEPTNNLDVESREAIERC
                     LQSWAGTLVIVSHDRYLLDRICTTTISIEDGILRTYYGPPSASMTETKTETEVGAEVG
                     VSARAKAELEPGAGPLREGDGAPTKGADDAAGTAHAADSANALVLENRLSVLSAKLAD
                     ASASEAERADAERDFLTISRALAQMRRK"
BASE COUNT          680 a         1053 c         1126 g          746 t
ORIGIN      
        1 atggacaagg tgaccagaga gcagggcttc tcctgcaggg gaagtgatgt atcctgtgag
       61 cgccagaaat cgctgatggt cgagaagatc gcccgcgaga ttcgggcggt gagcaagcgg
      121 aacggaaagg ttctggtcgt ggcgggtccc gcggtgatcc acacgggggc cggcaagcat
      181 cttgcggcgc ttatcgacgc gggctatgtc aacgttttct ttgccggcaa tgccgttgcc
      241 gtgcacgatg tggagtcggc gctgcatgga acatcgcttg gagtgtacct caaggacggc
      301 acatctgcgc caatgggcca catgcaccac ctgctggcca ttaacaagat acgtgacctt
      361 ggggggctgc gacaggccgt tgaggccggc gtcctcagag agggagtcat gcacgcgcta
      421 gtgaagaaca acgttccctt tgtgctggca ggttctatta gggacgacgg cccgctgccc
      481 gatgtcatca cggattcggt tgaagcgcaa gaggcgatga ggaagggcat caagggcgta
      541 gagattgcgc ttatgatgtc gaccatgctg cattcgattg ccaccggcaa tctgcttcct
      601 gcgcgggtca agacgatctg tgtcgacatc aacccggcta cagtgacgaa gctcgccgat
      661 cgcgggtcgc accaggcagt ggggattgtc agcgacgtgg agtggttcct gaaggaactc
      721 cggtcttatc tagtggcgta gcttcgaaca taagtgttga cgggcccggc gcgggcgctt
      781 cgcaggcgaa atgcggagtg cacgcgaccg ggtctttttt catggcccac tgtcgggcta
      841 tttgcgtcgc atctgggcca gcgcccgcga gatcgtcagg aaatcgcgct ccgcatctgc
      901 ccgttcggct tcactggcac tggcatctgc gagctttgcc gatagtaccg acaacctgtt
      961 ctcgaggacc aaggcatttg cggagtctgc cgcgtgcgct gttccggcgg cgtcgtctgc
     1021 acccttggtg ggcgcgccgt caccttcgcg caggggtccg gctccgggct caagctcggc
     1081 ttttgccctg gcactgaccc cgacctcagc cccgacctcg gtctcggtct tggtctctgt
     1141 catcgaagcc gaaggtggcc cgtagtaagt acggagaatt ccgtcttcga tggatatggt
     1201 cgtagtgcaa atccggtcga gcagatatcg gtcatgcgag acaatcacca gagtgcctgc
     1261 ccacgattgg aggcacctct cgatcgcttc gcgcgattcc acatccagat tgttcgtggg
     1321 ttcgtccagg gccaggatat cgtaaccgcg cgcgatcatg cgcccgaaga tgacccgaac
     1381 cttttcgccc aggctcaaca cgcttacgcg tttgccgact gcactaccgc ggaagagcaa
     1441 cgctcccaga atgtcgcgga cttcttgtgg agagcgcgtg cttgcggcgg atgatacgct
     1501 ttcgaacacg gtgtgcgaag gatcaagctg ctccatggtc tggtcgaagt agaaccatga
     1561 agcggacggg cttctccaca ccgtgcctga cgtggacggt tccaggccga ggagcatacg
     1621 gagcagagtg gtcttgccgg acgcgttcgg cccgatgata gctaccctgt cgcctcgcat
     1681 aatcgccaca tccgccgcgc caaacagcgg cctatcgaag ctcttggata tccccttcgc
     1741 atgtatcagc ctgcgcccgg acttctgcgc ttgccccatc ctgagcacaa tcgcatctgc
     1801 ctctcgtggc ttcgcgatac gctgctggcg gaagcgctca agacgcgcga aggacgcgcg
     1861 ggcgcgggcc gcccctttgt ttgctcgggc cctgagaaag tcgttctgcc cggcacgcct
     1921 gtgagcctgg cgaaaccact ccatttctct ggacgccact tgcttgagcc ggtcctcttc
     1981 gcgctcctgt ttttcgtaca gcctccactc tgcctcgagt tcactgtcct ttcgagcacg
     2041 gaacgcggtg tagttgccgg tataggagcg cgcgccacga ggagtcaagt cgatgacgcg
     2101 ccgggcggta tggtcgagga agtagcggtc atgcgatacg agcagcactg cggcgcggga
     2161 ctcacggatc aagcgcgcta gaaggtccat cgccggcgca tccagatgat tggttggttc
     2221 gtccagcata atcaggtcgg gctgcgctgc ccatacttgg gctagtccca cgcgcctttg
     2281 ctggcccgca ctaagagttg acatcggcat gctcgctaag gcgggctcga taccgaaccg
     2341 gcccagtacc gccgagtcac gacagagatc cgaaggcgtc aggtcgccct ggaattgcag
     2401 agactgtggc aggtagccta gtattgcccc gggccgattc caaacggttc ctgagtcggg
     2461 gtccgtacgg ccggatatga tgtttagcag ggttgatttg ccacatccgt tcgggccgaa
     2521 caaggcgact ttctcgcctg cctcgatttc caatgataca tcgcggaaga gggtccgggc
     2581 accaaatgcc ttggcgagat ccctgactga tagagctagc atcttgccgc ctcccaacga
     2641 gtgatacaca gttgacgagt gggatgcggc gacttccgtg atggatgatc ggggtcgggc
     2701 tgaaagcagg gagcctcgtg cgatggtggc tcgacttgcc gggtatgatt gccgggcgta
     2761 aattgcccag tgcggactgc ccagtgcaga ctgcccagtg caaattgtct ggcgcagcgt
     2821 cgagcctatc gccccttatg cactacattc ggaagacgcc aacattcccg tgcatgcaga
     2881 cacgcgattg cagggcccgg cgttacggga cccgcgagaa cgcgccctgc ggttcaccta
     2941 gaatgtcgac tgcttcctca tgtcgcaaag gccctttctg agtagtggag cgaagtgcgg
     3001 acggctgtca aggccagacc ggtgctccgc ccacaggata ccatgtgggg acagtgcgtg
     3061 caaggttggc gacatcgtct gttcgcgaca tcgtccatct gggaccgcca acatgtcgcc
     3121 gacgggttcc gcggacctgt gccctgtggg ccaggatttc cggggcacct gtgtcaagcg
     3181 acacaggatg gccgaaaaag gtgtcccgta gggcacaggc tccgatatcc ggcgatcccg
     3241 cctatgccga ggtctgcggc gactgtttcg ccccaggctc cttagctggg gttttcgtaa
     3301 ccatggattc tgccgggctc gaaggccgcc gacgggctcc gcggacctgt gccctgtggg
     3361 ccaggatttc cggggcacct gtgtcaagcg acacaggatg gccgaaaaag gtgtcccgta
     3421 gggcacagga tccgatatct ggcgatcccg tctatgccga ggtttgcggc ggctgtctcc
     3481 ccgacgaact gcaactgtct tctacataca gcaatctata tacagtccta catattggcc
     3541 atgagtgaca ggatatttgc atgtgatgta gaaacttgac gttgtggggt gacctcatga
     3601 agaca
//
LOCUS       DAOTAC010000259         3590 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_20811_length_3590_cov_4.295332, whole genome shotgun sequence.
ACCESSION   DAOTAC010000259 DAOTAC010000000
VERSION     DAOTAC010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20811_length_3590_cov_4.295332"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="PLM74_09975"
     CDS             <1..622
                     /locus_tag="PLM74_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43434.1"
                     /translation="IAELDRLGREAHRRITGTLGNLAVRYLPSVQFEAPDVQAIAGAF
                     RDRLAQIRKVELLRGVTMCGPHRDDILFELDGVNVAAFGSQGQQRSTVLAVKLAEVEF
                     IRARTGDQPVLLLDDVLSELDERRRAHLLAEVVGKYQALVTSVEPGCVEIMPPGARVF
                     MISAGQIHAGEVAADAVPSEGCTEHDSGQARLQGPPALTGGDARVE"
     gene            525..1388
                     /locus_tag="PLM74_09980"
     CDS             525..1388
                     /locus_tag="PLM74_09980"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HON43435.1"
                     /translation="MLCRLKDALSTTVDRLGYRGRLRSLAAMRAWNEDIAPCLKTESR
                     ATFIRGKVLHVTVEKPVWAQQLTMMRQDLINRLNALAGEGAVADIRFRTGQIREALQW
                     GPATREEAAAGFGEGVATSVPDWTCVEPDVDALARAREAAMPITDDELRSRFEEFMLL
                     DAKYRIWARRNLSTGATQAADVLRREPWLSDDHLHSLVPAATPDDLMRARATVAAEFC
                     DEVRARIAPEAIAAGGGCGSDAAQRRVELRLLVESIAMLLTGRPPEQVDGNLVAYALG
                     EEFASYVRQAE"
     gene            1369..1734
                     /locus_tag="PLM74_09985"
     CDS             1369..1734
                     /locus_tag="PLM74_09985"
                     /inference="COORDINATES: protein motif:HMM:NF015958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HON43436.1"
                     /translation="MCDKPNRPDTPDKPHKAFEPMVMREKHWDAPVHWLYLGGDAVID
                     SREVVAILDGERGRRSTALTEYIRRNVEAGRAIDVSDDGSVKSVVITADRIYLSQVAP
                     PTVGKRANIIYLGEDEGEE"
     gene            1834..>3590
                     /gene="gyrB"
                     /locus_tag="PLM74_09990"
     CDS             1834..>3590
                     /gene="gyrB"
                     /locus_tag="PLM74_09990"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HON43437.1"
                     /translation="MESSGVRNNNNTQYNADQIQVLEGLEGVRKRPAMYIGSTDARGL
                     HHLVYEVVDNAIDEALAGFCDTIVVTIGADGSVSVEDNGRGIPVDIHPREGKPALEVV
                     LTMLHAGGKFGGGGYRVSGGLHGVGVSVVNALSESLEAWVRRDGRIYYQAFRRGATVA
                     PLKVKGPADSTGTTIRFRPDPDIFETLDFSFDTLSFRLREFAFLSAGTKIVLRDEREG
                     DDGRRENVFQYDGGIRSFVEFINRAKDPVHDDVIYVSRDLESVSVETAIQYNDGYSEN
                     MLCFANYVNTTEGGTHLSGFRSALTRSINDYARKANILKDNDENLVGDDVREGLTAVL
                     SVKLEDPQFEGQTKTKLGNSEMRGIVDSVVTEGLTVYFEENPGTARKIVDKCVSAARA
                     REAARKAKELTRRKSALEISSLPGKLADCTLRDPALCELYIVEGDSAGGSAKSGRDRR
                     FQAIMPIRGKILNVEKARIDKIFANMEIRAMITALGTGIGEDFDISKLRYSKVVIMRD
                     ADVDGAHIRTLLLTFFFRFMRPLIENGNILIAQPPLYMVRQGKKEHYAYSDRELQEVL
                     GSLGGGNYPVQRYKGLGEM"
BASE COUNT          788 a          957 c         1173 g          672 t
ORIGIN      
        1 catagcagaa ctcgaccggc tgggccgcga agcgcatcgc aggataacgg gcacgctggg
       61 caatctcgcc gtgaggtatt tgccgtcggt tcagtttgag gcgcccgacg tgcaggcgat
      121 tgcaggcgcc ttcagggatc gtcttgccca gattcgaaaa gtcgagctac ttcgcggagt
      181 cacgatgtgt gggcctcacc gcgacgacat tctgttcgaa cttgacggtg ttaatgtggc
      241 tgcgttcggc tcccagggtc agcagagatc gacggtgctt gcggtgaaac tggcggaggt
      301 cgagttcata cgcgcacgta ccggcgatca gccggtactg cttctggacg atgttctgtc
      361 ggaactggac gaacgccggc gggcgcacct actggcagag gtcgtaggca agtaccaggc
      421 tctagtcacc agcgttgagc ctggctgcgt tgagataatg ccgcccgggg ctagggtgtt
      481 catgataagc gcggggcaga tccatgccgg ggaggttgca gcggatgctg tgccgtctga
      541 aggatgcact gagcacgaca gtggacaggc taggctacag gggccgcctg cgctcactgg
      601 cggcgatgcg cgcgtggaat gaggacattg ctccttgtct caagaccgaa tcacgagcca
      661 ccttcatcag gggcaaggtg ctgcacgtga ccgtcgaaaa gcctgtgtgg gcacagcagc
      721 tcaccatgat gaggcaggac ttgatcaata ggctaaacgc gctggcgggg gaaggcgcgg
      781 tcgccgacat acggttccgc acaggccaga tacgggaagc actgcaatgg gggcctgcga
      841 cgcgcgagga agccgcggcg ggctttggcg aaggggtcgc tacttctgtt cccgactgga
      901 cgtgcgtgga acctgacgtc gatgcgctgg ccagggcgag ggaggcggct atgccgatca
      961 ccgacgatga gctgcgcagt cgattcgagg agttcatgct gttggacgcc aaatacagga
     1021 tatgggcgcg gcgcaacctg tcgacaggcg cgacgcaggc agcggacgtt ctcaggcgcg
     1081 aaccgtggct gtccgacgac catcttcatt ctctcgtgcc cgctgctacg cctgacgact
     1141 tgatgcgtgc acgggcgacg gtagcggcag agttctgcga tgaagtgcgc gcacgcatag
     1201 ctcctgaagc aatagccgcc ggcgggggct gtggctccga tgccgcgcag cgtcgtgtcg
     1261 agttgagatt acttgttgaa tccatagcca tgctgttgac aggccgacct cctgagcagg
     1321 tggacggtaa cctcgtggcc tacgctcttg gcgaggagtt tgccagctat gtgcgacaag
     1381 ccgaataggc cggatacgcc ggataagccg cataaggcct ttgaacccat ggttatgcgg
     1441 gaaaagcatt gggacgcgcc tgtgcactgg ctctacctgg gcggcgacgc agtgatcgat
     1501 agccgcgaag tcgtagccat acttgatgga gagaggggca gacgctcgac tgcactgaca
     1561 gaatacatac ggagaaacgt ggaggcggga agagcaatag acgtatcgga tgacgggtcc
     1621 gtcaagtcag tggtgatcac cgccgaccgg atctacctga gtcaggtggc gccgcctact
     1681 gtgggcaaaa gagccaatat catctacttg ggcgaggatg agggcgaaga atagccgggc
     1741 gttttggtgt ataattggtc tgatgggcgc cgtggtgcga actcatacac cacggcgatt
     1801 ttatgggttc gaaacgatca gggaggtagt tttttggaaa gctccggcgt acggaacaac
     1861 aacaacacgc aatacaatgc cgatcagatt caggtgttgg aggggctgga aggggtccgt
     1921 aagcgacctg ccatgtacat cgggagcacc gatgccagag gccttcacca cctggtctac
     1981 gaggttgtcg acaacgccat cgacgaggcc ctcgccggct tttgcgacac catcgtggtg
     2041 acgataggag ctgacggttc agtctcagtt gaagataacg gccgaggaat cccagtcgac
     2101 atacatccca gagaaggcaa gcccgctctg gaagtagttc taaccatgct acatgcaggg
     2161 ggtaagttcg gcggcggggg atatcgcgtg tcgggaggcc ttcatggcgt gggagtgtcc
     2221 gtggtgaacg cgctctccga gtctcttgaa gcgtgggtgc gccgcgacgg ccgcatctac
     2281 tatcaggcgt tcaggcgcgg agccacggtt gcgccgctta aggtgaaggg gccggccgac
     2341 agcacaggaa ccacaatcag attccggccg gatccagata tcttcgagac gctggacttc
     2401 agcttcgata cgctctcctt caggttgagg gagttcgcgt tcctcagcgc aggcacgaag
     2461 attgtgctca gggacgaacg cgaaggcgat gacgggcgca gggagaatgt gttccagtac
     2521 gacggaggaa tccgctcatt cgtcgagttc atcaacaggg caaaagaccc tgtgcatgac
     2581 gatgtcatat acgtctctag agacctggaa tcggtatcgg tggagaccgc cattcagtat
     2641 aacgacggct attcagagaa catgctctgc ttcgcaaact acgtgaatac tactgaaggc
     2701 gggacacacc tgtcggggtt cagatcggct ctcacccggt ccataaacga ttacgcccgg
     2761 aaggcaaaca tcctcaaaga caacgacgag aacctggtgg gcgacgacgt gcgcgaaggg
     2821 ctcacagccg tgctcagcgt caaactggag gacccgcagt tcgaagggca gaccaagact
     2881 aagcttggca acagcgagat gcgcggcata gttgactccg ttgtgactga agggcttacc
     2941 gtgtacttcg aggagaaccc gggcacggca cgcaagatcg tcgacaagtg tgtttcggca
     3001 gcccgggccc gcgaagcggc acgcaaggcc aaggaactca cccgcaggaa atctgctctg
     3061 gagatatcgt cgcttcctgg gaagcttgcc gactgtacgc tgcgcgatcc ggctttgtgc
     3121 gagctctaca tcgttgaggg cgactctgca ggcggttcgg ccaaatccgg tcgcgaccgc
     3181 aggttccagg cgatcatgcc tatcaggggc aagatcctca atgtcgagaa ggcacgcatt
     3241 gacaagatct ttgccaacat ggagattcgg gcgatgatca cagctctggg caccgggata
     3301 ggcgaggact tcgacatctc caaacttcgc tacagcaagg tcgttatcat gagagatgcg
     3361 gacgtggacg gcgcgcacat acgcacactt ttgctgacgt tcttcttccg gttcatgagg
     3421 ccgctcattg agaacgggaa catcctaata gcgcaaccgc ccctgtacat ggtgcgccag
     3481 ggcaagaagg agcactacgc gtacagcgac cgagagctgc aggaggtgct aggcagcctg
     3541 ggaggcggca actacccggt gcagcgctac aagggtctcg gcgagatgaa
//
LOCUS       DAOTAC010000260         3589 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_20820_length_3589_cov_4.452462, whole genome shotgun sequence.
ACCESSION   DAOTAC010000260 DAOTAC010000000
VERSION     DAOTAC010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20820_length_3589_cov_4.452462"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(217..1329)
                     /locus_tag="PLM74_09995"
     CDS             complement(217..1329)
                     /locus_tag="PLM74_09995"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HON43438.1"
                     /translation="MFIHRAHLATKTLRILIVLAVASFALTGCGPVRNVGSVQGTITD
                     AVTLLPISGAKVTLINAAILTATTGTDGSYRFPSVAAGTYEVKVTKAGYVDRLGDGVT
                     VTKGATTTASMNMIMPADLEVLLYDDSILDNHFAAEALDALGITFTQVSDSVGLVDEM
                     TNHPWRLIVADHCNYPSSKAMLDCLSDYVDMGGHLAISSWDPTVSASSRAHALWEKLG
                     ADFSGFADGDVLAEDPFTVFRWAADHVIFRSPNAVTNISVNYVSRYNFEGVVAHESAG
                     GMAIAGITPAYQAGQGLVFVTDSNLTVFNSFLLDCVQDGAEPGDDTDSDADGVADAEE
                     LWQNEIVYLLSWTDPAAIQCPPIGPASLSAGGRNSK"
     gene            complement(1625..2296)
                     /locus_tag="PLM74_10000"
     CDS             complement(1625..2296)
                     /locus_tag="PLM74_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43439.1"
                     /translation="MGYSRPAGTWSRGALAALVIMACAATLLGAGCLGRSALRDEVMS
                     FFPDNEGFTWTYFGFAEYGHKMSIDRITNTKSPGNVVYEISGAVFDMSDGESGRDFSF
                     DLEYVFTQEDVRERILHKDVFPHRIDGLVMLKAPVKQGQNWDFKAEDGSRVDAKVTRT
                     GKDPEDDLAFCEVEYTTPDASMPDGLYRETRVFKKGLGVVAFKSTILPDVEFNYSLTS
                     TSGPR"
     gene            complement(2360..2944)
                     /locus_tag="PLM74_10005"
     CDS             complement(2360..2944)
                     /locus_tag="PLM74_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HON43440.1"
                     /translation="MCSDDLDPVCERLIRAIGIADPFRRRMYVLGVVTRALESDGVAA
                     PILVGGGAVDFYTLGGYATQDIDVVIAARERLDAVLKELGFSKERGQRHWYSPDLDVA
                     IEAPDDTLAGSLERVAVVDVDDLQVQVIGIEDLIMDRLRGYVHWQSISDREWAGRLTA
                     LHEASIDWEYLNYRAKTEGLLEALDSVSRECGFS"
     gene            complement(3269..3589)
                     /locus_tag="PLM74_10010"
                     /pseudo
     CDS             complement(3269..3589)
                     /locus_tag="PLM74_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511628.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6530 family protein"
BASE COUNT          737 a         1111 c          959 g          782 t
ORIGIN      
        1 ggccctcctt aggtagtcca cttctgaaac cgcccggtgc ccgccaagcg ggttcggccc
       61 gggcagaggc ctcggtctgc aattcgacaa aagactatcc tatcctccag ctgccttggg
      121 aatcggcttc ttaggaggcg ttcaaggacg aatagctagg gcggggacac gccccgccct
      181 aagaccgaga tactcttgcc cacggctcga tcttgatcac ttgctgtttc ggccgcctgc
      241 gcttagcgaa gcgggaccaa ttggagggca ttggatggct gcaggatctg tccatgacag
      301 caggtacacg atttcgtttt gccacagctc ctcagcatca gccacaccgt ccgcatcact
      361 atcggtgtca tcacctggct ctgcaccgtc ttgcacacag tcgagaagga aactgttaaa
      421 tacagtgagg ttgctgtcag tcacgaacac aagaccttgt ccggcctggt atgcgggggt
      481 tatgccggca atggccatac ctcctgcgct ctcatgggcc acgactcctt caaagttgta
      541 cctagatacg tagttcacgg agatgttcgt aacagcgttg ggcgatctga agatcacatg
      601 atcggcagcc cacctaaaca ccgtgaatgg atcctcagcc aggacgtcac catcggcgaa
      661 tccagagaag tccgcgccaa gtttttccca aagcgcgtgt gcccttgagg acgcgctgac
      721 tgtgggatcc cacgaggaaa tggcaaggtg cccacccatg tcaacatagt cagagaggca
      781 atccagcatg gccttgctgc tagggtagtt gcagtggtcc gccacgatca ggcgccatgg
      841 atggttcgtc atctcatcca caagcccgac cgaatcactg acctgtgtga aagtaatccc
      901 cagcgcatca agggcttcgg ctgcgaagtg attgtcgaga atggagtcat cgtacaggag
      961 cacttctagg tcggccggca tgatcatgtt catcgaggct gttgtagtgg cacccttggt
     1021 taccgtcacc ccgtctccca gcctgtcaac ataccctgcc ttcgtcacct tcacctcgta
     1081 ggttccagct gcaaccgaag ggaatctgta ggatccgtcc gttccggtag tggcagtgag
     1141 tatcgcggcg ttgataagcg tcaccttagc gccggatatg ggcagcaaag tgactgcatc
     1201 cgtgatggtt ccctgaaccg accccacgtt tctcacgggc ccgcaccccg tgagagcgaa
     1261 ggacgcaaca gccaggacaa taagaatgcg cagagttttg gtggcgagat gtgctctgtg
     1321 gatgaacaaa tggattcgcc tccctcttgt tgtgtacgct gatcgtgagc tcatgcggga
     1381 ttcacatgat ctgcgcttcc cggcccgcgg gtgcagtcgc acgccctggc catcgatgcc
     1441 cgttactacc ggcccatgcc caatgcgcaa ggcggtgtac tgtgcgcgag tgcgggtttg
     1501 atggctggat tattccgcgt gttatgtgtt cttcctgctg actatcggct gatctcgaag
     1561 ttaattcgag tgtggaatgt ggagcgcgtc accgcgtcta gctgcgacat aggtgaggcg
     1621 ccgctcagcg cggcccagac gtcgatgtga ggctatagtt gaactccaca tcagggagaa
     1681 tcgtcgactt gaaggcgacg acgcccagac ccttcttgaa gactctggtt tcccggtaca
     1741 gcccgtctgg catgctcgcg tcgggagtgg tgtattcgac ttcgcagaat gccaagtcat
     1801 cttcagggtc cttccccgtc cgggtgacct tggcgtcgac cctgcttccg tcttcggctt
     1861 tgaagtccca gttctgtccc tgtttgaccg gggccttgag catcaccaga ccatctatcc
     1921 ggtgagggaa cacatccttg tgaagtattc gttcgcgcac atcctcctgg gtgaagacgt
     1981 actcaaggtc aaagctgaag tcgcgcccgg attcgccgtc tgacatgtcg aacacagcgc
     2041 cggagatctc gtagaccacg ttacccggcg acttcgtgtt ggttattcga tcgatactca
     2101 tcttgtgtcc gtactcggca aacccaaagt aggtccaggt aaagccctca ttatcaggga
     2161 agaacgacat gacttcgtcc cgcagcgcag agcgcccgag acaccctgcc ccgagtagcg
     2221 tcgctgcaca cgccatgatt actaaggcgg cgagagcgcc ccggctccat gtaccggctg
     2281 gcctggagta gcccatgaaa ctagccacga gttgggatcc cccgatcaga aaccggattg
     2341 acgatgctga tgcgggccat cacgaaaatc cgcattcgcg actcaccgag tccagcgcct
     2401 ccagcagtcc ttcggttttg gcccgatagt tcagatattc ccagtctatc gaggcttcat
     2461 gcaaggcagt cagccttccg gcccactctc ggtcggaaat cgactgccag tgaacgtagc
     2521 ccctcaaacg atccatgata agatcctcga taccgattac ctgtacctgc aagtcgtcaa
     2581 catcaacaac tgcaacccgc tcaagcgagc cggcaagagt gtcatccggc gcctcaatcg
     2641 ctacgtccaa atctgggctg taccagtgcc tttgcccccg ttccttcgaa aaccccagtt
     2701 ccttgaggac tgcatccagc ctctcgcgag cggcaatgac tacgtcgata tcctgggtag
     2761 cgtacccgcc aagcgtgtag aagtccaccg cgccgcctcc caccaagatg ggcgcagcga
     2821 cgccgtccga ctccagcgcg cgtgtcacaa cacccaacac gtacatgcgg cgtctgaacg
     2881 ggtcggcaat gccaatagct cggatcaacc gttcgcaaac cggatcgaga tcatccgaac
     2941 acacggagac gatacctcct ctcggctacc ctgacgagct cgccggttga aacggcgcga
     3001 tccaatctgg ccttggccag ccgagtgagg acggcgacct cttccgggtt cctcgggcgg
     3061 cgccttacgg cccgatggga gctgccctct ccgcttccca cttgagccat gcccgccgcg
     3121 cggccggcct attgtgtgca gtatactcat caagatcgaa tcgtctggag cccaaaggta
     3181 ttccactccc cgccatgccc tagtagtgta catccgtctt ctgcgaatcc gccggtcctc
     3241 ctgcagatcg aacctcagtc tccggctctc agtagcccat tcccttcaaa atggtagaca
     3301 gcgcgctcag ccccccccta tcaattcgtc atccctgctg atgcagtctc tgaatagctt
     3361 gatgccctca tcgatcatgg gattatcagt acatacctca atagtcatgg catcgccctg
     3421 caggcctacc cgatcgatgg cggtcttttc ggggtcgtag agcttctcat agcggtatgc
     3481 ctccgtgaag tgctgtcgcg ccctgcatat gagcagcgca agatctagtg ctctgtgcag
     3541 cggcagttcc tccgattgcc tcgaccattt ttcaccggtg tgcctccac
//
LOCUS       DAOTAC010000261         3576 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_20903_length_3576_cov_3.565464, whole genome shotgun sequence.
ACCESSION   DAOTAC010000261 DAOTAC010000000
VERSION     DAOTAC010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20903_length_3576_cov_3.565464"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="PLM74_10015"
     CDS             <1..601
                     /locus_tag="PLM74_10015"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF013916.2"
                     /note="catalyzes the reversible formation of fructose
                     1,6-bisphosphate from glycerone phosphate and
                     D-glyceraldehyde 3-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HON43441.1"
                     /translation="LDSGVGLIIRITGGFTVLGGRFEEAMISSVTHALKLGATGVAVT
                     VKFGHEREDEFTRQASLVADDCYDWGMPLMTEVWPAGKNVGKPSDLSAVKLGARAAAE
                     YGTDIVKTFYPGTEEGCAEVVAGCPVPVVVLGGEKADAPLDTFRMVESAIRAGAAGVA
                     MGRNVWGQPDPTRMIEALRGIVHDGWSAEQADTFFGRQG"
     gene            605..1543
                     /locus_tag="PLM74_10020"
     CDS             605..1543
                     /locus_tag="PLM74_10020"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HON43442.1"
                     /translation="MGRVEGACLLRVQGVLASLSPSEQRVAVCVLEHPRKVVQSTITD
                     LARECEVSESTVVRFCKSLGLDGYKEFRIAIAQELGALVPELAAAEMGTAREVKELAQ
                     DVFSNNIRALEATLGSLDYEAVARATEALARARRVDFYGAGPSNVVAMDAYIKFMRIG
                     LSAGFNSNSYLQAISAAVLTNEDVAVGISYSGSTRDTVEALSIARKAGATTVAITNFS
                     DMPISDASDIVICTKADESLFQGGAMASRTAQLAVIDLIFAGTVSRRSDVFAEQFDKT
                     KTALLMKLRGAAGVPGQAGRNGRASAPHRGEHDTKQ"
     gene            1561..>2162
                     /locus_tag="PLM74_10025"
     CDS             1561..>2162
                     /locus_tag="PLM74_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007933621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HON43443.1"
                     /translation="MNIFVLMKQVPGTSNVKMDEKTGTMIRTEHENVINPLDENALEE
                     ALRLRQAVPGSIVTAISMGPPPAARALREALAMGADSAVLLCHRALAGSDTLATARAL
                     AGAVKHVAGGDVGSRDLVLCGERATDGETGQVGPMIAALLDVCLATYVRSIGLEDAEG
                     ASPTVVAERVVEDGFERVRLSLPCVITVVKDINQPGFPTLA"
     assembly_gap    2163..2172
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2173..2392
                     /locus_tag="PLM74_10030"
     CDS             <2173..2392
                     /locus_tag="PLM74_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007933621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta"
                     /protein_id="HON43444.1"
                     /translation="AGKIRARKAEVRMLSHEKLGLGADAVGLSGSPTRVVRIFRPSFA
                     RNTVLHAQDGSGAAVDALVDLLVDRGIL"
     gene            2398..3498
                     /locus_tag="PLM74_10035"
     CDS             2398..3498
                     /locus_tag="PLM74_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HON43445.1"
                     /translation="MHSITCVGDLGRVLDMGHAGVMVVGEQRRGEVHPVTFELLARGR
                     QLADRLGAELSCVVIGSGLETGAREIIERGADMVYLVDDPALAWFLPGLYARALARLI
                     QSEMPEIVIAAATTSGRTLMPLVAARLGTGLTADCTELDIDTKERLLLQTRPAIGGNV
                     MATIKTPQARPQMATVRPKSARPLPRDAARVGRVVQVKLETKDCGAVGGGTIGCGAGS
                     GRGQGLEVVLGSVRDATQEVNVQDAEVIVTGGKGLKNKDNFRMIEELASLLGAAVGAT
                     RDVVDLGWISYPHQIGLSGKTVAPRLYIAVGVSGSVQHLAGMQTAETIIAVNKDKDAQ
                     IFKVADFGIVGDLFEVVPEMIEKIRNRTAAGR"
BASE COUNT          676 a          944 c         1285 g          661 t
ORIGIN      
        1 gctggactcg ggcgtgggcc tgatcattcg gattacaggg ggcttcaccg tgctgggagg
       61 cagattcgag gaggctatga tcagctcagt gacgcatgcg ctcaagctcg gcgctacggg
      121 agtggccgtg actgtcaagt tcggccatga gcgtgaagat gagttcacgc gccaggcttc
      181 gctggttgcc gacgactgct atgactgggg tatgccgcta atgaccgagg tgtggccggc
      241 aggcaagaat gttggcaaac cttccgatct ttccgccgtc aagctgggcg cacgtgcggc
      301 ggccgagtac ggaactgata tagtcaagac cttctaccct ggtaccgagg agggctgtgc
      361 cgaggtagtg gcgggttgcc cggtgccagt ggttgttctg ggcggcgaga aggccgatgc
      421 gcctctcgat accttccgca tggtcgagtc ggcgatccgg gcgggcgcag ctggcgttgc
      481 catggggcgc aacgtgtggg gccagccgga cccgacgagg atgatcgaag ctctgcgtgg
      541 catcgttcat gatgggtgga gcgcagagca ggcagacact ttcttcggaa ggcagggttg
      601 acgtgtgggc agggttgaag gggcctgtct gctgcgagtg cagggagttc tggcctcgct
      661 gtcgccaagt gagcagcggg tggcagtgtg cgtactagaa caccctagga aggtcgttca
      721 gtctacgata actgatctgg cgcgcgaatg cgaggtgtcg gaatccaccg ttgtccggtt
      781 ctgcaagagc ctcggcctgg atggatacaa ggagttccgc atcgccatag cccaggaact
      841 gggggctctc gtgcccgagc ttgccgctgc cgagatgggg accgctcgag aagtcaaaga
      901 gctggcccaa gatgtgtttt ccaacaatat cagggctctg gaggctacct tgggttcgct
      961 ggactacgaa gcggtagccc gggccaccga ggcgctggct cgtgcacgga gggtggactt
     1021 ctacggggca ggcccatcca acgtcgtagc gatggacgcc tacatcaagt tcatgcgtat
     1081 aggtctgtcg gccggtttca actccaactc gtatctacag gcaatatcgg ctgcagtgct
     1141 aaccaacgaa gatgtcgcgg tcgggatctc atattccggt tccacgcgcg acaccgtgga
     1201 agctctcagc atcgccagga aggccggggc gactactgtg gccataacga acttcagcga
     1261 catgccaata agcgacgctt ccgacatcgt catatgcacc aaggcggatg aatcgctgtt
     1321 ccaggggggt gccatggcct cgcgcacggc gcagttggcg gtgatagatc tgatttttgc
     1381 gggcaccgtg agccgaaggt cagatgtgtt cgcggagcaa ttcgacaaga ccaagaccgc
     1441 cctcctaatg aagctgaggg gcgccgccgg cgtaccgggg caggcgggca ggaatggccg
     1501 agcatcggcg ccgcatagag gcgagcacga cacgaaacag tgagagcggg ggcatggcag
     1561 atgaacatct tcgtcttgat gaagcaggtg ccgggcacgt ccaatgtgaa gatggacgag
     1621 aagaccggca ctatgatcag gactgagcat gagaacgtta tcaacccgct ggacgaaaat
     1681 gccctcgaag aggcgctgcg attgaggcag gctgtgccgg ggtcgattgt gaccgcgatc
     1741 agcatggggc ctccgcctgc cgccagggcg cttcgcgagg ctctggccat gggcgccgac
     1801 agtgcggtgc tgctctgtca ccgtgccctg gcgggatcgg acacgctggc tacggcgcgc
     1861 gccctggccg gcgccgtgaa gcatgtggcg ggcggcgatg tcggctcgcg tgacctcgtc
     1921 ttgtgcggcg aacgtgccac cgatggcgag accgggcagg ttggcccgat gatcgccgcg
     1981 ctcctcgacg tgtgcttggc gacgtatgtc cggagcatag ggctggagga cgctgagggc
     2041 gcttcgccca cagtggtggc ggaacgggtc gtagaagacg gatttgagcg cgtgaggctg
     2101 tcgcttcctt gtgtgattac cgtggtcaaa gacatcaacc agcccggctt cccgactctg
     2161 gcnnnnnnnn nnggccggca agatacgcgc gcggaaagcc gaggtgcgca tgctcagcca
     2221 cgagaagctg ggcctgggcg ccgatgctgt cggcctgtcg ggatcgccta cgagagtagt
     2281 tcgaatattc aggcccagct ttgcgcgaaa cacggttctg catgcccagg acggctcggg
     2341 cgcggctgtg gacgctctcg ttgatctgct cgttgatcgg gggatcttgt gatggatatg
     2401 cactcgataa cgtgcgtagg ggatttgggg cgtgtgcttg acatgggaca tgcaggcgtg
     2461 atggttgtcg gggagcagcg ccgaggagag gtgcatccgg ttacgttcga gcttttggcg
     2521 cggggcaggc agcttgccga caggcttggc gcggagctat cgtgcgtggt gatcgggtca
     2581 gggctggaaa ctggcgcccg cgagatcatc gaaagaggcg ccgacatggt ctacctggta
     2641 gatgatcccg ccttagcatg gtttctgccg gggctctacg cccgggcgtt agcgaggctt
     2701 atccaaagcg agatgcccga gatcgtcata gccgctgcta cgacttctgg gcgcacgttg
     2761 atgcctcttg tggcggccag gttgggcacc ggcctcactg cagactgcac cgagctggac
     2821 atcgatacca aagagcgcct tctgctgcag acgcgaccgg ccattggcgg gaatgtgatg
     2881 gctacgatca agacgcctca ggcgaggccg cagatggcga cggtgcgccc caagtccgcg
     2941 cgtccgctgc cgcgcgatgc ggcgcgggtg ggcagggtcg tccaggtgaa gctagagacg
     3001 aaggattgcg gtgcggtagg gggcggcacg attggctgtg gcgccgggtc cgggcgcggg
     3061 caagggctcg aggtcgtact tgggtcggtt cgcgatgcga cgcaggaagt gaatgtgcag
     3121 gacgccgagg tcattgtgac aggcggcaag ggcctgaaga acaaagacaa cttccggatg
     3181 atcgaggaac tcgcgtcttt gctcggggcg gcggtggggg ccacgcgcga tgtggtagac
     3241 ctggggtgga tcagctaccc gcatcagatc ggcctatcgg gcaagaccgt tgcgccgcgc
     3301 ttatacattg cggtaggcgt gtcggggtcg gtgcagcacc tggccgggat gcagacggcg
     3361 gagaccataa tcgctgttaa caaggacaag gacgcgcaga tcttcaaggt tgccgacttt
     3421 ggcatagtcg gagacctgtt cgaggtagtg ccggagatga ttgaaaagat caggaatcgg
     3481 acggcggcgg gaaggtgacc ggcgagatga cgaccgatga gacgaatgag acaaacaggt
     3541 atgggtgtgc gccggccgag aagaggttca gcccgg
//
LOCUS       DAOTAC010000262         3554 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_21065_length_3554_cov_3.751929, whole genome shotgun sequence.
ACCESSION   DAOTAC010000262 DAOTAC010000000
VERSION     DAOTAC010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21065_length_3554_cov_3.751929"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..654
                     /locus_tag="PLM74_10040"
     CDS             52..654
                     /locus_tag="PLM74_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43446.1"
                     /translation="MKLPDVNVKLVAASLAVALALLFGGNALVRVTRVSIPVERALGH
                     MAEVKSHSIESTPYGAVLNVELVDVSDLSETFRSLTMAAAHLAGQDGVWIRIADSRDD
                     TLRDVYYRLHFSLQEAIATGSFEDLERRSEESAREAGLTGARVVVDSEYVYVSMAHGA
                     NQLYEVVPRRPYDAGATTGSRSVARSDPRSFGAAGGVRIW"
     gene            648..2138
                     /locus_tag="PLM74_10045"
     CDS             648..2138
                     /locus_tag="PLM74_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HON43447.1"
                     /translation="MVRDIAIGVWAAALIFCVVSGYDPALIVLAGGVAALAQLLLSGR
                     ITGQRFEVANRACAGQKVRGLTFGDVGGQETAKRELVEALEFIRNEDSAVRLGIRPLR
                     GILLSGPPGTGKTLLARAAANYTDAVFISAAGSDFVEMYAGVGAKRVRDLFSTARATA
                     VAQHKKTAVIFIDEIDVLGGVRGKHSSHLEYDQTLNQLLVEMDGLVSASRDGVRVLVV
                     GATNRADLLDPALLRPGRFDRVVNVDLPDREGRRRILEIHTKRKPLADDVDLGRVAAE
                     AFGFSGAYLESLTNEAAIMALRAGRARITMDDFSEAMEKVIMGEKINRRPDVDELLRV
                     AVHEVGHAVASERLKPGSVAAVTVSSRGKALGYVRQAPKSDIYLETRMELEDRMCTML
                     AGAVAEELILGERSTGASQDIEQAVETARKIVNCGLSDLGIISEEDIPRDKYHDAIWH
                     LVRAQEDRTRSLLSEHSELIREIAGELMDVERIDGDAFRRRLAVAV"
     gene            2176..3477
                     /locus_tag="PLM74_10050"
     CDS             2176..3477
                     /locus_tag="PLM74_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HON43448.1"
                     /translation="MMAEILSVGTELLLGQVVDTNAAYIARRLAELGVTLYRKTTVGD
                     NLARVSAAVREALERADIVIATGGLGPTEDDLTREAVADALSVRLVESPEVLAGLASF
                     FASRGARMTENNRRQALRPEPGAGGLIPNPIGTAPGVLFESGDKRVICLPGVPREMER
                     MMDSWVVPYLRDCRRGPDSAQVIKSKVVRTFGIGESALEELIVDLAHGHDNPTVATYA
                     GGGECQVRVTARADSEPEADALIQSVLAPLGARLGRLVYGYDADTLEEAVGRQLAEAG
                     LSMACAESCTGGLVSAMLTNVPGSSAYLDRGVVTYSNRAKVEQLAVDEKTLASHGAVS
                     RDTAVEMATGVARIARVDIGLSVTGVAGPGGGTDAKPVGTVHFAMADMGRPSVTTARL
                     RFGGDRAAVRTRAAKFALDLIRRHLVGDRQDHPADHPANGQ"
BASE COUNT          685 a          977 c         1234 g          658 t
ORIGIN      
        1 tataatcatc cgtgcagctt gagggctgtt taccagaggg gagggtacgc cttgaagcta
       61 ccggatgtga atgtcaagct agtggcggcg agcctggctg tcgccctggc tttgcttttc
      121 gggggaaacg cgcttgttcg cgtgacacgg gtttcgattc ccgtggaacg cgcgctgggg
      181 catatggccg aggtcaagtc gcattcgatt gagtcaactc catacggcgc tgtgctcaat
      241 gtcgagctcg tcgatgtgtc ggatctttcc gaaacattca gaagcctgac tatggctgca
      301 gcacatcttg ccgggcaaga cggggtgtgg atacgcatcg cagattcccg cgacgatacc
      361 ctgcgagacg tctactatcg tcttcatttc tcattgcagg aggccatagc taccggctca
      421 ttcgaagacc tggaacgccg gtccgaggag tcggcgcgag aagcgggcct taccggcgcc
      481 cgggttgtgg tagactcaga gtacgtatat gtgtccatgg cgcacggcgc aaaccagctg
      541 tacgaggtgg tcccgaggcg accatatgac gccggggcaa cgaccggatc aaggtcggtc
      601 gcgcgctctg atccccgctc ctttggggca gcgggaggtg tgcggatatg gtaagggaca
      661 ttgcgatcgg cgtatgggct gcggctctga tcttctgtgt ggtctccgga tacgatccgg
      721 ccctgattgt gctggccgga ggggtggccg ctcttgcgca gttgcttctg tcggggcgca
      781 ttaccggcca gcggtttgag gtagcaaacc gggcctgcgc agggcagaag gtgcgcgggc
      841 tgactttcgg agacgtggga ggacaggaga ctgccaagcg tgagctcgta gaagctctgg
      901 agttcataag gaatgaagac agtgcggttc gtctggggat acgcccactc cgcggcatac
      961 tgctgagcgg tcctcccggc accggcaaga cgcttcttgc cagagctgcg gcgaattaca
     1021 cggatgcagt gtttatatcc gcagccggca gcgatttcgt ggagatgtac gcaggcgtcg
     1081 gggccaagcg ggtgcgtgac ctgttcagca cggcgcgcgc gacagctgta gcgcagcata
     1141 agaagacggc ggtcatattc atagacgaga tcgacgtgct gggcggtgtg cgcggcaagc
     1201 actccagcca tctagagtac gatcagacac tcaaccagct cttggtggag atggacgggc
     1261 tggtgtcggc atctcgcgac ggggtgaggg tgttggtggt cggtgccaca aaccgtgccg
     1321 accttctgga cccggctctc ctcaggccgg gcaggttcga tagggtggtc aatgtggacc
     1381 tgcctgatcg tgaaggccgc cgccggatac tggagataca caccaagcgg aagccactcg
     1441 cggatgatgt ggacctaggg cgcgttgcag ccgaagcctt cggcttctcc ggcgcctatc
     1501 tggagagctt gaccaacgag gcggcgatta tggcgctgcg ggcagggagg gcccggataa
     1561 ccatggacga cttctccgag gcgatggaga aggttatcat gggcgagaag atcaacaggc
     1621 gtccggatgt ggacgagctg ctcagagtgg cagtacatga ggtcggccac gcagtcgcga
     1681 gcgaacggct gaagccgggt tccgtggctg ccgtcacagt atcgtcgcgg ggaaaggctc
     1741 tggggtacgt gcgccaggct cctaagtccg atatatacct ggaaacccgc atggagctgg
     1801 aggacaggat gtgtaccatg ctcgcgggcg ctgtggcgga agagctcatc ctgggcgagc
     1861 ggagcacagg cgcgtcacaa gacattgagc aggctgtgga gacagcgcgc aagatcgtca
     1921 actgtggcct gtcggaccta gggataatca gcgaagagga cataccgcga gacaagtacc
     1981 atgacgccat atggcaccta gttagggcac aggaagaccg cacccggtct ttgctgagcg
     2041 aacattcgga gctgatccgg gaaatcgccg gtgagctcat ggacgtggag agaatagacg
     2101 gagacgcttt caggcgccgt ctggccgtag cggtgtaacg cggacctggc ggcgcgcagg
     2161 gaggcaggct acgagatgat ggcagaaatc ctttcggtcg gcactgaact gctgctcggg
     2221 caggttgtcg acacgaatgc tgcatacatc gcgcgtcggc tggcagagct gggcgtcacc
     2281 ttgtatcgaa agaccaccgt aggcgacaac ttggcccggg tatccgctgc ggtgcgtgag
     2341 gcgcttgagc gcgcagacat cgtgatcgcc accggcggcc tcgggccgac ggaagacgac
     2401 ctcacgcgcg aggcggtggc ggatgcgcta tcggtgaggc tggtggagag ccccgaggtt
     2461 cttgccggcc tcgccagttt cttcgcctct cgcggggccc gcatgaccga gaacaaccgc
     2521 aggcaggcct tgcgccctga gccgggggcg ggcgggctca taccgaaccc gataggcact
     2581 gccccgggcg tcctgttcga gtcaggcgat aagcgggtga tatgcctccc gggcgtgccc
     2641 cgggagatgg agagaatgat ggactcctgg gtcgtgccgt acctgcgcga ctgccgtcgc
     2701 gggcccgaca gcgctcaggt gataaagtcc aaagtcgtac ggacctttgg aatcggcgaa
     2761 tcggctctgg aggagctcat agtcgacctg gcccatgggc acgacaaccc gactgtggcc
     2821 acctatgccg gcggaggaga gtgtcaggtg agggtcaccg cccgggccga ctctgagccc
     2881 gaggcggatg ctctcataca gtctgtgctg gcgcctcttg gcgctcgcct tgggcggctg
     2941 gtatatggat acgacgccga cacattggaa gaagcggtgg gtcgccagct tgcggaggcg
     3001 gggcttagca tggcttgcgc tgagtcgtgc accggagggc tggtatccgc catgctcacc
     3061 aacgtgcccg gaagctccgc atacctcgac cgcggcgtcg ttacctatag caaccgtgcc
     3121 aaagttgagc agcttgcggt ggatgagaag accctggcct cgcacggggc tgtgagccgc
     3181 gatactgcag ttgagatggc cacaggggtg gcccggattg cccgagtgga catcggcctt
     3241 tcggttaccg gagtagcggg cccgggggga ggcacagatg ccaagcctgt cggcacagtc
     3301 catttcgcca tggccgacat gggccgtcct tcggtgacaa cagcccgact gaggttcgga
     3361 ggagatcgcg ccgctgtgcg gacccgagcg gccaagttcg cgctggatct cataaggagg
     3421 catctggtgg gagaccgcca ggatcacccg gcagatcacc ccgctaatgg gcaataataa
     3481 cccggatggg cggtgctact tgtggaggaa tatcgacgcg agtctggaat tccaattatc
     3541 agaacgttca tcgc
//
LOCUS       DAOTAC010000263         3522 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_21261_length_3522_cov_6.086530, whole genome shotgun sequence.
ACCESSION   DAOTAC010000263 DAOTAC010000000
VERSION     DAOTAC010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21261_length_3522_cov_6.086530"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..341
                     /locus_tag="PLM74_10055"
     CDS             114..341
                     /locus_tag="PLM74_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HON43449.1"
                     /translation="MIKTLTAHGNSAALIIDKPILGLLDITMSTPLKIVTDGKNLIIS
                     PVRDAGRDELFRAALAKVNAEHESTLRKLAE"
     gene            387..530
                     /locus_tag="PLM74_10060"
     CDS             387..530
                     /locus_tag="PLM74_10060"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HON43450.1"
                     /translation="MDSAVFMTLDEAVAVHTDQVKRYGGHPFIDGNKRTALACALFGS
                     LPA"
     gene            complement(834..2720)
                     /locus_tag="PLM74_10065"
     CDS             complement(834..2720)
                     /locus_tag="PLM74_10065"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HON43451.1"
                     /translation="MGGEKRKQRASIRWKITGAIVLVVLLMGAASLLLYREVSVLGKA
                     TGRLASDEYHYLELAEEVRYFDLRVSDAMKAVLLQPDDPSRGEELKLAGEEFAHVVAE
                     AALLATSDEDIQLFEKMRSYLEDFESYQSDLLEMARDWGADVAMTIYESEYAPMRLWS
                     ADALSKFAQRRREAMVSGFQDAIDRSLVSQKMSLAAMGAGLVASLMLGWRISGRIARP
                     IRRIMEAADTVAGGDLTAEVPATGTGDEIERLTESVREMMMGLRRLIQGVLEMTEEVA
                     GASAELSASAEESSLAVDQIAGTVQELAAAGQRQGADAVSVTGSARRLEATIAEVAQG
                     TERQAASARESARSVDQAGAALSESRTVLEAVMDSVTHNSELARRGSESVDAVSARMD
                     EIVSATQDVERRIRELEGHSHEIGRILEVIRDIADQTNLLALNAAIEAARAGEHGRGF
                     AVVADEVRKLAERSSAETKAIGALVQSIVESTGSAVRAMAGGARAVGAGKEVSSQASA
                     ALEEIAYSSSQLGKLVEKLIAALVTVEGATSAVKQHMSGIMGVAEANTAAARSMRADA
                     GQVQEDVSGVASLAEENAASIQEVSASAEQVAASLKGMADSTGILTDMAQKLRLEVGR
                     FKVS"
     gene            2924..>3522
                     /locus_tag="PLM74_10070"
     CDS             2924..>3522
                     /locus_tag="PLM74_10070"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HON43452.1"
                     /translation="MKRFEHIHEFLSKHKWEYVAGVACLILVDALQLVIPRLLGRLTD
                     SLRAGTLTSDGLRSVVIVIMGIAGAIAVGRFLWRIFINGTARLLDFYLRNKLFAHLER
                     LPARYYNEHKTGDLMAHATNDIPAIRQTFGQGFILLTDAIFMTGATLIILFRTTPLRL
                     ALLSLLPFPFLALGTTLFSRLVGPRFRAVHEAFSDLTERA"
BASE COUNT          665 a         1199 c          892 g          766 t
ORIGIN      
        1 ttccgaaatg cctgctttcc atgcactaca tgcacctatg ccccacatct gtttgaccca
       61 gtccgttctt ggctatacat cgtaattaca atgtatggac cggagggatg gctgtgatca
      121 agaccctgac cgctcatgga aacagcgcgg cgctgatcat cgacaaacca atactgggac
      181 tgctcgatat caccatgagc actcctctga agatcgtcac cgacggcaaa aacctgatca
      241 tatcgccagt gcgcgacgcg ggacgcgatg agctgttcag ggcagcgctc gccaaggtga
      301 acgcagaaca cgagtctacg ctcaggaagc tcgcggagta gcacgcagtc tgcccggaaa
      361 gcacaggctt tgcgaggtga ttctgcatgg acagcgcagt cttcatgacc ctggacgagg
      421 cagtcgctgt ccacacggac caggtcaaac gatatggcgg gcatccgttc atcgatggca
      481 acaagcgaac cgccctagct tgcgctctgt tcggctcgct ccctgcttaa gggacttttg
      541 cgttctctcc gatgtcaaac tcgggatgca tcacctattg tccgttccgt cgacgcatcg
      601 tcgaaaacgt gctcataaaa tcgacatcta gtttttccca tcggccctgg gccggtcgct
      661 gcgcctttcc cgctgccaag cttcgcttcg tctgtgatca aaaccgcacg tacccacggg
      721 gatgggttcg tctcactttg ggtttggttg gccatagacc ctatcagcgc cgcgccccat
      781 ctgcccgcct ttccaggtga tagacccggt cgcgctggaa gctctcaccg agcctacgat
      841 accttgaacc tccccacctc caaccgtagt ttctgggcca tgtctgtcag tattccggtt
      901 gagtcggcca tccccttgag cgaggccgcc acttgctcgg cagatgccga cacctcctgg
      961 atcgaagcgg cgttctcctc ggccagagag gccaccccgc tcacatcctc ctgaacctga
     1021 ccggcgtctg cccgcatgct gcgtgcggcc gcggtattcg cctccgccac tcccatgatc
     1081 cccgacatgt gctgtttcac cgctgacgtg gcgccctcaa ccgttacgag tgcggcaatc
     1141 agcttctcca cgagcttgcc gagctgtgac gaactgtacg ctatttcctc aagcgccgca
     1201 gacgcctgag acgaaacctc tttgcctgcc cccaccgccc gcgcgccccc cgccatggct
     1261 cgaacggcgc tgccggtcga ctccacgatg ctctgcacca gcgcccctat ggccttggtc
     1321 tctgccgagg atcgctccgc caattttcgg acttcatctg ctaccacggc aaatccacgc
     1381 ccgtgttcgc cggcgcgtgc cgcctctatg gctgcattca gcgctagcag gttagtctgg
     1441 tccgcgatgt cccttatgac ctcgagaatc ctgccgatct catgcgagtg tccttcgagt
     1501 tcgcggatgc gtctctcgac atcctgcgtg gccgagacga tttcgtccat acgggctgac
     1561 acggcatcga ccgactccga accgcgacgg gccagttcgg aattgtgggt gacagagtcc
     1621 atcacagctt ccagaaccgt acgagactcg ctcagggctg caccggcctg atcaactgaa
     1681 cgcgcggact cccgcgcgct cgcagcctgg cgctccgttc cctgtgccac ttcggcaatg
     1741 gtagcttcta gcctcctcgc ggatccggtc acgctcacgg catcagcccc ctgtcgctgg
     1801 ccagccgctg caagctcttg cacggtaccg gctatctgat ccacggccag tgacgattcc
     1861 tcggctgagg ccgacagctc cgcgctggcg ccggccacct cttcggtcat ctccagcact
     1921 ccctgaatca accggcgcag acccatcatc atctcgcgca ccgattctgt cagccgctca
     1981 atctcatcgc cagtccccgt cgccggcact tcagccgtca ggtccccgcc tgccaccgtg
     2041 tcggccgcct ccatgatacg ccgtatcggg cgggctatcc gcccggaaat ccgccacccc
     2101 agcatcagcg aggctaccag gccggcgccc atagccgcaa gcgacatctt ctgcgaaacg
     2161 agcgatcgat ctatcgcgtc ctgaaacccg ctcaccatgg cttcccgccg cctctgggcg
     2221 aactttgaaa gcgcatcggc agaccacagc ctcataggcg catattccga ttcgtatatc
     2281 gtcatcgcaa catctgcgcc ccagtcgcgc gccatttcca gcagatccga ctgatatgat
     2341 tcgaagtcct ccaggtacga cctcatcttc tcgaacagct ggatgtcctc atcgctagtt
     2401 gccagtaacg cggcctcggc caccacatga gcgaactcct cacctgcaag cttaagttct
     2461 tccccacggc tcggatcatc cggctgcagc agtacggcct tcatcgcgtc cgacacccta
     2521 agatcgaagt atcgtacctc ctccgccagc tccaggtagt gatactcatc agacgcgagc
     2581 cttcccgtgg ccttgccgag aaccgatacc tcccggtaga gcagtaacga agccgcaccc
     2641 atcaggagca cgacaaggac gatagcgcca gttatcttcc atcgtatact ggctctctgt
     2701 ttccgctttt ctccgcccat caccaatgct tcctccctcg cgattggttc tcttgccttc
     2761 acagaacatt atcggcacat atctgctgtc tgtttaccag cattgcacac ccagaatgcc
     2821 catatgctgg ataatgctgg cgaatgcgtc tttcatctgg gtgcattgtg ggctataatg
     2881 atgtggtgtc gcgtgcgata gccgcagaat cgaggagtgg ccgttgaaac gcttcgagca
     2941 tatacacgaa ttcctgtcca agcacaaatg ggagtacgtt gcaggggtcg cctgcctcat
     3001 cctcgttgac gcactgcaac tggttatccc gcgtctcctt gggaggctca cggacagtct
     3061 ccgcgcagga actctcacca gcgatgggct gcgcagcgtc gttattgtga tcatgggtat
     3121 agccggcgcc atagcagtcg gtcggttcct ttggcgcatt ttcatcaatg gcacggcccg
     3181 gctgctggat ttctacctga ggaacaagct ctttgctcat ctggagcggc ttccggctcg
     3241 ttactacaac gagcacaaga cgggagactt gatggcgcat gccaccaacg acatcccggc
     3301 aattaggcag acgttcggac agggattcat cctgttgact gacgccatct ttatgacagg
     3361 tgccacacta atcatactgt tcagaactac gccgctccga ctggctctgc tgagcctgct
     3421 gcccttccct ttcctggccc tcggcactac cctcttctcc aggttagtcg gcccgcgttt
     3481 ccgagccgtg cacgaggcct tctccgatct caccgagcga gc
//
LOCUS       DAOTAC010000264         3464 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_21644_length_3464_cov_5.305075, whole genome shotgun sequence.
ACCESSION   DAOTAC010000264 DAOTAC010000000
VERSION     DAOTAC010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21644_length_3464_cov_5.305075"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="PLM74_10075"
     CDS             complement(<1..694)
                     /locus_tag="PLM74_10075"
                     /inference="COORDINATES: protein motif:HMM:NF015042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HON43453.1"
                     /translation="MMHSTGKETIAVAMSGGVDSSVAAALLVSEGFAVIGVTMKLWHS
                     SIDGSFDNRCCSVESVEAARRVARRLGIDHIVLNVSEEFHRMVVSDFVDQYSRGLTPN
                     PCVVCNARIKFGELHRRLLALGVDHVATGHYARIDVDGASGRKLLFRPRDRSKDQTYV
                     LYRLTQSQLARTMFPLGGMTKPEVRAVARSMQLETAENPESQDVCFVPQGRYRDFLIH
                     ERPELGAPGPIIS"
     gene            826..2697
                     /locus_tag="PLM74_10080"
     CDS             826..2697
                     /locus_tag="PLM74_10080"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HON43454.1"
                     /translation="MNLRRKLVFVFAGVALVSSMAVGSLLYIQSVKTNREMLGQVALA
                     AVRTAAPSIDGDIHSRVVSYASEETAEYSAIADQLREISRHNPDLIFVYTLFRSTPDK
                     IFFAVDIGTGDDHTEFGYEYEPDEAVLGALAGNASVTPGFVSDEWGTFITACAPIRDS
                     SGQIVGVLAGDLDGRGIVARERRMLIQVVAIVAVVLVCTMALSALLAGRTVKPLLNVA
                     QMVRDIAEGEGDLTRRLDATAKDEIGHLAAWFNRFVDGLQSMVMAMMQTSSAVAQTAQ
                     ELSSASSEVASASRQIALSTAEVAQGAQAQSKNAQAGAAQLRAIKAGAQESAGGADDQ
                     EKASADASKAAEGMVGALRGVAVGLQETVGASEENARTARSGIKAVNAVLESMDAVKD
                     RTERAVASIRALDSQSREIGKILDMIVDIAAQTNLLALNAAIEAARAGEHGRGFAVVA
                     DEVRKLAERSASEAKAVAQLVEGVLRATDESVALIGESAQETEKASELSSDARQALER
                     VLDSAQRSYELVAGLAREIQALQTGVEQVGKTVSVATEMAKANRRIAQDMADRAASAE
                     RSTESSAAVSEQTAAAAQELSASAEQVSASVEEMTAQVASLAAQAQNLAQLAARFKV"
     gene            2897..>3464
                     /locus_tag="PLM74_10085"
     CDS             2897..>3464
                     /locus_tag="PLM74_10085"
                     /inference="COORDINATES: protein motif:HMM:NF016350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HON43455.1"
                     /translation="MYTTDIELSGHLIDTMLLSRVIDAIAEMDAEFSLRETRIGRTAD
                     DTSYARIEIGAPTCERLDEVIDRVKELGATPVDGQPVTLATVVKDGVCPDGFYSSTNI
                     ETYVNVAGKWVPVDDIEMDCAVVVDPVNFKARCCPVGALSRGEQVVIGHKGVRVAAMD
                     RVAEEQGFSFMGSDVSSERQKSLMVEEIA"
BASE COUNT          729 a          926 c         1149 g          660 t
ORIGIN      
        1 gcgatattat cgggccggga gctcccagct cggggcgctc atggatgaga aagtcccgat
       61 atctgccttg gggcacgaag cacacgtcct ggctttccgg gttctccgcc gtctccagct
      121 gcatggaacg ggcgaccgct ctcacctcag gttttgtcat cccgcccagc gggaacatgg
      181 tgcgcgccag ctggctctgg gtcaatctgt aaaggacgta cgtctggtcc ttgctcctgt
      241 cacgtgggcg gaagagcagt ttgcggcctg atgcgccgtc aacatcaatg cgggcataat
      301 ggcctgtggc aacatggtcg acgccgaggg ctaggagcct gcgatggagc tcgccgaact
      361 taatccgggc attgcacacg acgcatggat tgggagtgag tccgcggcta tactggtcca
      421 cgaaatccga cacgaccatg cggtggaatt cctcggacac gttgagcacg atgtggtcta
      481 tgccgagtcg gcgagcgact cgccgagcgg cttcgaccga ctcgacggag cagcaccggt
      541 tgtcaaacga gccgtcaata gaggagtgcc acagcttcat cgttaccccg ataactgcaa
      601 aaccctctga taccagcagg gcagcggcta ctgacgagtc tacgcccccg ctcatcgcca
      661 cggcaatagt ctccttgcct gtactgtgca tcatgtgctc ctcctccggg aaacgtgctc
      721 ccgttcgata aagtgttgcg acaaccttcc gatattagta tagacaggca gttgcgataa
      781 gtcactggcg gcaatgtaag atcacaacga acgggggcgc ggacgatgaa cctcaggcgc
      841 aaactggttt tcgtgttcgc aggagttgcg ctagtctctt cgatggctgt tggaagcctg
      901 ttgtacatcc agtcagtcaa gacaaacagg gaaatgctcg gccaggtggc cctggccgct
      961 gtgcggaccg ccgctccgag catcgacgga gacattcaca gccgcgtggt gtcgtacgct
     1021 tccgaggaga ctgcggagta cagcgccatc gcagaccagc tgagggagat aagccgtcac
     1081 aatcccgacc taatcttcgt atacacactc ttcaggtcga cgccggacaa gatctttttt
     1141 gcggtggata tcggcaccgg ggatgatcac actgaattcg ggtatgaata cgagccagat
     1201 gaggcagtgc ttggggcgct tgccggcaac gcgtcggtta ctcccggctt cgtctcggac
     1261 gaatggggca cgttcattac ggcttgcgct cccatacgtg attcatcggg gcaaattgtg
     1321 ggcgtactcg ccggtgatct ggacggaagg ggtatcgtcg cgcgcgagcg ccggatgttg
     1381 attcaggtag ttgccatagt ggcagtggtg ttggtatgca ccatggcttt gtcggcgctg
     1441 ctggccggtc gcacagtgaa acccttgctc aacgtggcac agatggttcg tgacatcgcg
     1501 gagggcgagg gtgaccttac ccgccgcctg gatgcgaccg cgaaagacga gattggccat
     1561 ctcgcagcat ggttcaacag gtttgtagat gggctgcaat ctatggtgat ggccatgatg
     1621 cagacatcgt ctgccgtggc acagaccgcc caggagcttt ccagcgcgag cagcgaagtc
     1681 gcttcagcca gccggcagat tgctctgagc acggccgaag tggcccaggg cgcccaggca
     1741 cagagcaaga atgcgcaagc cggtgcggct cagctgcgcg caatcaaagc cggcgcccag
     1801 gagtcggccg gaggggccga cgaccaggag aaggcttcgg cagacgcgtc gaaggctgca
     1861 gagggtatgg taggggctct gagaggggta gcggttgggc tgcaagagac tgtcggcgcg
     1921 tccgaggaga acgcgcgcac ggcacggtcg ggcattaagg ccgtgaatgc agtactggaa
     1981 agcatggacg ccgtcaagga tcgaaccgag cgggctgtgg ccagcatacg cgcgcttgac
     2041 agccaatcga gagaaatagg taagattttg gatatgatag ttgatattgc cgctcagacc
     2101 aacctccttg ctctcaacgc tgcgatcgag gcggcgcgtg ccggagagca tggcagaggc
     2161 tttgcagttg tagccgacga ggtgaggaag ctcgccgagc gctcggccag cgaagccaaa
     2221 gccgtcgccc agctggtgga gggcgtgctt cgtgccactg atgaatcggt ggcgctgatc
     2281 ggggagagcg cgcaggagac tgagaaggcc agcgagctat cgtccgacgc ccggcaggcg
     2341 ctcgaaaggg ttctggattc tgcccagagg tcgtacgagc ttgtggcagg cctggccagg
     2401 gagatccaag ccctacaaac tggtgtggaa caggtgggga agaccgtatc agtggcaacg
     2461 gagatggcta aggcgaatcg gcgcatcgcc caggacatgg cggacagggc cgccagtgcg
     2521 gagcggtcta ccgagtcgtc ggcggcagtg agcgagcaga ccgctgccgc ggcgcaagag
     2581 ctatcggcaa gcgctgaaca agtgagtgcg tcggtggagg agatgacagc tcaagtggca
     2641 tcgctcgccg ctcaggcaca gaaccttgct cagctggcgg cgaggttcaa ggtctagcgg
     2701 cgggtgttcg ggttggcgag atatgggccg gacgatggcg gggcaacccg cgcgcccggc
     2761 tttccttgtt ccggcggacg atatctgata caattgtgtg gagaatttag gttgacattt
     2821 cggaatctgg gtatacatta tgcatgcctg atgcataaat gccaaccaag aatgtccgtt
     2881 aaggagtgat cagccggtgt acacaaccga catcgagctc tcgggacatc tcatcgacac
     2941 catgttgctg tcgcgcgtta tcgacgcgat agcagaaatg gatgcagagt tcagcctacg
     3001 cgaaacgagg atagggcgca ctgcggacga cacgagctat gccaggattg agattggggc
     3061 gccaacgtgc gagcgactcg acgaagtcat cgacagagtt aaggaactag gcgccacacc
     3121 cgtcgatggc cagcccgtta ccttggccac agttgtcaag gatggggtgt gccccgatgg
     3181 gttctactcc agcacgaaca ttgagactta tgtgaacgtg gcgggcaagt gggttcctgt
     3241 tgacgacatc gagatggact gcgctgtggt ggttgatccg gtgaacttca aggccaggtg
     3301 ctgccctgtg ggggcgcttt cccggggtga gcaggtggtc atcggccaca agggcgtacg
     3361 ggtcgctgcg atggacaggg ttgccgaaga gcaaggcttc tccttcatgg gaagtgatgt
     3421 atccagtgag cgccagaaat cgctgatggt cgaggagatc gccc
//
LOCUS       DAOTAC010000265         3456 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_21692_length_3456_cov_4.120847, whole genome shotgun sequence.
ACCESSION   DAOTAC010000265 DAOTAC010000000
VERSION     DAOTAC010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21692_length_3456_cov_4.120847"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(588..1472)
                     /locus_tag="PLM74_10090"
     CDS             complement(588..1472)
                     /locus_tag="PLM74_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HON43456.1"
                     /translation="MQTNADGRPRRCSGPGLTGRPGPLAHSDRLRSSGEHETFLRSLA
                     RRRMLVGTARAMLLVAFVVLWEVAARFGWINAFVVSQPSRALATAMSMARRGELWIHL
                     GMTVGETAIGFTVGTLIGIVAAVMLWWSDLLSDILDPYIVVLNSIPKVALGPIFIVWL
                     GTTVTAIIAMAISISVIVTIMMVYVGFREVDPDRVKLLRTFGATRLQILSKVVLPASV
                     PVMIAALKVNVGLSMVGTIVGEFLVSKAGLGFLIVYGGQVFNMSLVMSSVILLSIVSV
                     LLYYGVSALEQAVLRTRE"
     gene            complement(1435..2229)
                     /locus_tag="PLM74_10095"
     CDS             complement(1435..2229)
                     /locus_tag="PLM74_10095"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON43457.1"
                     /translation="MTYHTREAETVALADISLDVAPQEFVAVVGPSGCGKSTVLSLVA
                     GLIRPTSGSVEVCGEAVTGPSGHVGYMLQQDCLYEWRTILGNALLGLEVKRAVTRESV
                     EMVRHMLDAYGLGEFERHYPRHLSGGMRQRAALIRTLATRPDVLLLDEPLSALDYQTR
                     LEVSEDLYRIMRAERLAALMVTHDIAEAIAMSDRVLVMSKRPGRIKSGHRISLTCERQ
                     TPIGRREAPEFRAYFNAIWKELTEDGHAVAGGSEHADERGWAPAAV"
     gene            complement(2283..3323)
                     /locus_tag="PLM74_10100"
     CDS             complement(2283..3323)
                     /locus_tag="PLM74_10100"
                     /inference="COORDINATES: protein motif:HMM:NF024772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HON43458.1"
                     /translation="MFRVRLNVARIARVAAILALAMVFALVFVSGAALGKVGRGYTIV
                     RLSEVVRSIFYAPQYVALAQGFFEDEGLKIDLSTAWGADKGAAALISGACDIGFFGPE
                     AAIYIFNQGAPDYLIGFAQLTQMDGSFFLARTGMPEPFSWEHVRGKTIVGARLGGVPE
                     MVLEWVLKKHGIEPFKDVKIITSLAFAAAPGAFAAGMGDYIAQFEPSMTELEMAGQGK
                     IVASMGVEGGEIMYTVYHVRRSQLVRDRDMLIRFTRALARGMKWVEDHTPAETAEVIR
                     PYFPDTKLEVIARIVQRYRDQGTWPKTPEVSPSGFMRLQEVMASAGELKATVPANALM
                     TTEITRAALKTK"
BASE COUNT          708 a         1179 c          907 g          662 t
ORIGIN      
        1 ggtcgccggc ccggtagtca gtcagcttct gcggtttggg tacccatggt acggcgtcag
       61 cggtttcccg ctcagctgca atatgtggat atccagggcg cttcggcctt tgtatccggg
      121 ccgatccatg gatatggtta acgtatagaa gaaatgtacc cggttttccg aggtacctgc
      181 atagaatatg cccggttttg atcggtcagg cgggaaatcc agccgtattg gggttttctg
      241 ggttgcctga acccgtctag ggagcgtccc cggggagatt cgtagggtac acgagcgtaa
      301 ccacattatg taatcctcgt ctgcctgcag atttgccgga catccgactc cagaataagg
      361 aggggccgtt gccggcgcga attctgacga ttagcgccca ccaaaccagg taggatctga
      421 acaggctgac caaaaaccct gttcaaaaac gaacggggtg gtgcgaacta ccaccctcgc
      481 ttaagcagta ttgcagcaat tcctacagcg aatagggccc agtggacagt agatactcac
      541 actatcgagt ccgtttcacg tcatgcagtc accctcgacc agtcgcgcta ctccctagtc
      601 cggagcaccg cctgctccag cgccgacacc ccgtagtaaa gaaggacgga aacgatggag
      661 agcaatatca cggaactcat gaccaggctc atgttgaaca cctgtccgcc atagacgatc
      721 aagaaaccca gtcccgcctt tgacaccagg aattcgccta cgattgtacc gaccatcgat
      781 agccccacgt taaccttgag cgcggcgatc atcaccggca cactcgccgg cagcactacc
      841 ttgctgagta tttgcagccg cgtagcgccg aaggttctga gcagtttcac ccggtccgga
      901 tccacctcgc ggaagcccac gtacaccatc attatcgtca cgatgaccga tatcgaaatg
      961 gccatggcga tgatagcagt gacggtcgtc cccagccata cgatgaatat cggtcccagc
     1021 gccaccttgg gtatggagtt gagcaccacg atgtacgggt cgagaatatc cgacagcaga
     1081 tccgaccacc acagcatcac cgccgccacg atcccgatta gcgtgccaac cgtgaacccg
     1141 atggccgtct cgcccacggt catccccagg tgaatccaga gctccccgcg gcgcgccatc
     1201 gacatcgctg tggccaaagc cctggacggt tgcgacacga cgaaggcgtt gatccaccca
     1261 aagcgtgccg caacctccca tagcaccaca aacgccacca gcagcattgc tctggcagtg
     1321 cctaccagca tgcggcgcct cgccagactt cggaggaacg tctcgtgttc gccggacgac
     1381 cggagccgat ccgaatgtgc cagcgggccc gggcgcccag tcagcccagg cccactacac
     1441 cgccgcgggc gcccatccgc gttcgtctgc atgttcgctt cctcccgcga ctgcgtggcc
     1501 atcctcggtc agctccttcc agatagcgtt gaaataagcg cgaaactcag gcgcttcccg
     1561 gcgaccgatg ggcgtctgcc gctcacaggt gagactgatc ctgtggccgg acttgatcct
     1621 tcccggcctc ttgctcatga cgagcactct gtcggacatg gctatcgctt ccgcgatatc
     1681 gtgcgtcacc atgagcgcgg cgagccgctc cgccctcatg atccggtaca ggtcctcaga
     1741 cacctcgagc ctggtctgat aatccaaagc cgacaggggt tcatccagca gcagcacatc
     1801 cggacgggtc gcgagcgtcc ggatgagcgc tgcgcgctgc cgcattccgc ctgacagatg
     1861 gcgcgggtag tgtcgttcga actcgcccag gccgtacgca tcaagcatgt gcctgaccat
     1921 ttcgacactc tcgcgcgtca ccgccctctt cacctcgagc ccgagaagag cgttgccgag
     1981 tatggtgcgc cattcataaa gacagtcctg ctggagcatg tagcctacgt gtccgctggg
     2041 ccctgtaacc gcctcgccgc aaacctcgac gctgccggac gtgggcctta tcaagccggc
     2101 taccaatgat agcaccgtgc tcttcccgca tccactgggc ccgactacgg ctacgaattc
     2161 ctgcggggcc acatccaggc ttatgtcggc gagcgccacg gtttcggctt cccgggtgtg
     2221 gtaagtcatc cacacccggt ctagcctaat cgcgctggag cccgccacgt tcactccacc
     2281 gctcatttcg tcttcaacgc ggcccgcgtt atctccgttg tcatcagcgc atttgccgga
     2341 accgtcgcct ttagctcacc cgccgacgcc ataacctcct gtagccgcat gaaaccgctc
     2401 ggcgacacct cgggcgtctt gggccaagtt ccctggtccc gatagcgctg cacgattcgg
     2461 gcgatcactt caagcttcgt gtccggaaag taaggccgga tgacctcggc cgtctcggcc
     2521 ggcgtgtggt cctccaccca cttcatgccc cttgcaagcg cccgagtaaa tcgaatcagc
     2581 atgtcgcgat cccgcaccag ctggctccgt ctcacatggt agacggtata catgatctcc
     2641 ccgccctcaa cccccatgga cgcaacgatc ttgccctgcc ccgccatctc cagttcggtc
     2701 atggacggct cgaactgggc aatgtagtcg cccatcccgg cggcgaaggc tcccggagct
     2761 gccgcaaagg ccaggctggt tatgatcttc acgtccttga acggctcgat cccgtgcttc
     2821 ttcagcaccc actccagcac catctcaggc accccgccta ggcgtgcccc gacgatggtc
     2881 ttgcccctca cgtgttccca gctgaaaggc tcaggcatcc cggttcgggc caaaaagaag
     2941 gacccgtcca tctgagtaag ctgagcaaag ccgatcagat agtccggggc cccctggttg
     3001 aaaatataga tcgccgcctc cggcccgaag aacccgatat cgcacgcacc agagatcaac
     3061 gcagccgcgc ccttgtcagc gccccatgcg gtagacagat cgatcttaag accctcatcc
     3121 tcgaagaaac cctgcgccaa cgccacatac tgcggcgcgt agaatatgga cctcacgacc
     3181 tccgatagac gcacaatagt gtacccacgt ccgaccttgc ccagcgctgc gccggacacg
     3241 aagaccaacg cgaacaccat agcgagcgcc agtatcgccg cgactcgggc gatcctcgcc
     3301 acgttcaacc tcacgcgaaa catgctccca cctccatgac gccagtcatg tattgcagga
     3361 gcatattatg ctgcggtcac gcttgctgac tatctgttct ccgggttcgc ctcagctgcc
     3421 ggctcagctc ccgacccctt cgccgccctc aatacc
//
LOCUS       DAOTAC010000266         3407 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_22044_length_3407_cov_5.384845, whole genome shotgun sequence.
ACCESSION   DAOTAC010000266 DAOTAC010000000
VERSION     DAOTAC010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22044_length_3407_cov_5.384845"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="PLM74_10105"
     CDS             complement(<1..597)
                     /locus_tag="PLM74_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003337244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HON43459.1"
                     /translation="MRINHNLSALNAWKNLTVNDGGQTKTLERLSSGLRIGRAADDAA
                     GLSISEKMRGQISGLNQATRNAQDGISLLQTAEGALGETHSILQRMRELAVQAASDTV
                     TNDDRAEIQKEVDALALELGRISTDTEFNTQKLIDGTFSNKVVHIGANADQSLRVSIE
                     AMSGNSLGVAFGSVLNNVQLVSGGDNAYAMVTSAELEHD"
     gene            complement(701..1828)
                     /locus_tag="PLM74_10110"
     CDS             complement(701..1828)
                     /locus_tag="PLM74_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003335947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HON43460.1"
                     /translation="MRINHNLSALNAWKNLTVNDGGQTKSLEKLSSGLRIGRAADDAA
                     GLSISEKMRGQISGLNQASRNAQDGISLLQTAEGALGETHSILQRMRELAVQSASDTV
                     TDGDRLEIQKEADALALELNRIAGTTEFNTQNLLAGKFADKTVHIGANSDQNLKVSVS
                     DMSAKALAVHQNINFGADYASGTQFGGDKLRVFGTVNTAGGAANAVQVRVTVTNAGDD
                     SEAATLEWLDTAGNVLATNAGIEIDSAPGAATYGLTFQEGTVNVVLNDTWTIDLMNKG
                     IDMSTQSAANTAITTINTAINTVSSERSKLGAVQNRLEHTIANLATSAENLTAAESRI
                     RDVDIAVEMMNFTKYQILAQASTAMLAQANAKPQAVLQLLR"
     gene            2641..2832
                     /locus_tag="PLM74_10115"
     CDS             2641..2832
                     /locus_tag="PLM74_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43461.1"
                     /translation="MRILFSDKELAEACMNPQAGQRRWGKSVYKKIVKRLSEFGAAES
                     LHDIATIKGALRRGKARTY"
     gene            2854..>3407
                     /locus_tag="PLM74_10120"
     CDS             2854..>3407
                     /locus_tag="PLM74_10120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HON43462.1"
                     /translation="MNSDGFIEYVPAVVTPPGDTLKEALDEIGMTQAELARRMGRPKK
                     TINEIIRGKAAITHETALQLERVLDIPAHFWRALEDNYQQHILKSRDLRLLEEHADWL
                     DEIPMASMVREGWAEPGDTAAENVANALRFFGVASVDAFRDTWGAAVAAFRQSPSFQS
                     NRWAIAAWLRKGEIEAARLRCAPYS"
BASE COUNT          625 a         1030 c          969 g          783 t
ORIGIN      
        1 gtcatgctca agctcggcag atgtgaccat cgcatacgca ttgtcgcccc cgctaaccaa
       61 ctgcacgttg ttcaggacgg acccaaatgc cactccaagc gagttgccgc tcattgcttc
      121 tatggacacc ctcaaggact gatcggcgtt ggcgccgata tggaccacct tgttgctgaa
      181 tgtgccatcg atgagtttct gggtgttgaa ctcggtatcc gtcgagatgc ggcccagctc
      241 gagtgcgaga gcgtcgactt ccttctggat ctcggcgcgg tcgtcgttgg ttactgtgtc
      301 ggacgccgcc tggactgcca gctctctcat gcgctgcaga atcgagtgcg tctctccgag
      361 agcgccctct gctgtctgca ggagcgagat gccgtcctgg gcattccgcg tggcctggtt
      421 aagaccactg atctggccgc gcatcttctc cgagatcgac aggccggccg cgtcatctgc
      481 ggcgcgtccg atcctcaagc cactggagag tcgctcgagg gttttggtct gcccgccgtc
      541 attgacggtc aggttcttcc acgcgttcag cgcggagagg ttgtggttga ttcgcatgtg
      601 cgtctacctc cttgtgcccg gttgccccgc atccatgcgg gtgggcctac gtgcatctga
      661 gcattcctac ccttgcttct cacctagtcg ctccggcgcg ctacctgaga agttgcagta
      721 ccgcctgggg cttcgcattg gcctgcgcca gcatcgccgt ggatgcctgc gccagtatct
      781 gatatttggt gaagttcatc atctcgaccg cgatgtccac atccctgata cggctctcgg
      841 cagcagtcag attctcagcc gacgtcgcca ggttggcgat ggtgtgctcc aacctgttct
      901 gtacagctcc gagcttggac cgctcactcg acacagtgtt gatcgccgtg ttgatcgtgg
      961 taatcgccgt gttcgcagcc gactgcgtcg acatgtcgat gcccttgttc atcaagtcta
     1021 tggtccaggt gtcgttgagc actacgttga cagtgccttc ttggaacgtc agcccgtaag
     1081 tcgctgcgcc aggagcagaa tcgatctcaa taccggcgtt ggtggccaag acgttccccg
     1141 ccgtatccag ccactcgaga gtcgctgcct ctgagtcatc tccggcatta gtcacggtta
     1201 ccctaacctg caccgcattc gcggcgcctc cggcggtgtt cacagtcccg aacacccgca
     1261 gcttgtcgcc gccgaactga gtgccgctcg catagtccgc cccgaagttg atgttctgat
     1321 gcactgccaa ggccttcgcg ctcatgtcgc tgacgctcac cttgaggttc tggtcagagt
     1381 tagcgccgat gtggacggtc ttgtcggcga acttgccggc caggaggttc tgggtgttga
     1441 actcagttgt gcccgcgatc cggttaagct caagggcaag ggcgtccgct tccttctgga
     1501 tttcgagtcg gtcaccatcg gtcacggtat cggacgccga ctggaccgcc agctctctca
     1561 tgcgctgcag gatggagtgg gtttcgccca atgcgccctc tgccgtctgc agcagagaga
     1621 tgccgtcctg ggcattccgg ctggcctggt taagcccgct gatttgtccg cgcatcttct
     1681 ctgagatcga taggccggcc gcgtcgtccg ccgcccttcc tatcctgagg ccgctcgata
     1741 gcttctcgag ggacttggtc tgcccgccgt cattgacggt caggttcttc cacgcgttca
     1801 gcgcagatag gttgtggttg attctcatgt ggatctacct ccttgtgtgg tccgcccggc
     1861 atccatgccg gggctgtcga ggttctgcga gcgcttcatc catatacagt ccacataccg
     1921 tttgccagtt acacgatttt catcaaacac ccccagtcga tcgaagtagt cacacccagc
     1981 ctagtatcaa tccggacatc ctcacgcctt accccaagaa aaccgttcgc gcgtcacaca
     2041 gagtatccac ggagtccttg ctggttccat tatcggcacg tgaacgccac gggtttaggc
     2101 ccagtcgccg gcaagccctg cgcacagtac ccatacaata atgcccctcg ctccttaccc
     2161 gatcgtgcag tacctggttc gcagcgaact cgtagagcct cctgaatcac cggcgccgtc
     2221 ttctagccca tcgacatcat gtgcgatcat cgcccctgag agcggtccat cacgcaatgg
     2281 cggatcgtcg gaatccccga acgcccatat gcgcaccccg gcaacgcaaa ctccctgcac
     2341 gagcatgttt ctatgcgaat ccgggggtgt gcttcacatg cactcgaatg acaacacctg
     2401 taatggcaat ggatatcctc tgctgccgcg ctgctgggct atcatcacag ccccggtcgt
     2461 ccttcctgtc ttccttgact cagttgacgt gggaggcgtg ggtgggccgt gttcccatca
     2521 atgggaagta cggtatgatc gccatgtccc ccggggtcgg caggctctca tcggcagtgc
     2581 cggcgcaacg gtatctagtt aacccctggg gttaattctg cgttgactgg aggcttattc
     2641 ttgcgtatac tgttctcaga caaggaactt gctgaggcat gcatgaaccc gcaagctggg
     2701 cagcgtcggt ggggcaagtc ggtctacaag aagatcgtca agaggctaag tgagtttggc
     2761 gctgctgagt ccctccatga tattgcgact atcaaaggcg cgctgcggcg cggaaaggcg
     2821 aggacatact gatcctggag gtgattgact atcatgaata gcgatgggtt catcgagtat
     2881 gttcctgccg ttgttacgcc tccgggcgat acgctcaaag aggcccttga cgagatcgga
     2941 atgactcagg ctgagctggc caggcgcatg ggacgtccga agaagacgat aaacgagatc
     3001 atcaggggca aagccgccat tactcacgag actgcattgc agcttgagag ggtgcttgat
     3061 atccccgcgc acttttggag agccttggag gacaactatc agcagcacat tctgaaatcc
     3121 agagatctgc gcctccttga agaacacgca gactggcttg atgagattcc gatggcctca
     3181 atggtgagag aaggctgggc tgaacccggc gacaccgccg ccgaaaacgt ggcaaatgcc
     3241 cttagattct tcggagtcgc cagcgtagat gcgttcagag acacttgggg ggcggcagta
     3301 gcagcctttc gtcagtcgcc gagtttccag tccaatcgct gggctattgc tgcgtggctg
     3361 cgcaagggcg agatcgaggc agccaggctc cgctgtgcac cctactc
//
LOCUS       DAOTAC010000267         3404 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_22064_length_3404_cov_4.347566, whole genome shotgun sequence.
ACCESSION   DAOTAC010000267 DAOTAC010000000
VERSION     DAOTAC010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22064_length_3404_cov_4.347566"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="PLM74_10125"
     CDS             <1..337
                     /locus_tag="PLM74_10125"
                     /inference="COORDINATES: protein motif:HMM:NF019998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HON43463.1"
                     /translation="YPVPSGMGDGPFYAACRPMDIRLSREVADAGVEARTGAGAGAGA
                     GGALKGLVTRVIYLGNIYEYRVQVGDSEIRVQQDSFEAFRHGVLREGEVCALSIDNIR
                     YYDDMEEVS"
     gene            379..2061
                     /locus_tag="PLM74_10130"
     CDS             379..2061
                     /locus_tag="PLM74_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HON43464.1"
                     /translation="MEGSRFNTGQLLLVISTVILIITVVLPVAMIVYNVFFYKWSFDW
                     SLFASMLTDPDNLAAMWNTVKISFFVTTLGTVVGLFFAWLIGRSDIPLKGLMKSLFVI
                     PYMFPPFIGAMAWGLLLAPRSGYINKMFMALTGGRTPLFNINTLAGIVFVELCYYFPF
                     VFIQVSGALERMDPTLEESARIAGANQMTVIRRITMPLVVPAIAAGAMLILISSLSHF
                     GVPAILGFSNGIFTLPTKIYELIYRASGSFEGIRRGASLSILLVAVVVLALALQRRVL
                     RAGRYDIIRGKSMRPMLIKLRGWKAPMLLVCFVFLAITVILPLFTIFGVGFLKAYGVP
                     MKLANMTLNNFKYVLFESRMARDSIRNSFFLSITSAVITMLVGTMIAYVIVKIQPRGK
                     GVLEVLGILPYSIPGIVLAVGVILTWSGAFYLNLYNTIWIILIAYIARYMAFSMKSAS
                     ASLEQVHDSLEEASRTCGASHFESLLDVTLPLIRPGMVAGFFLIFLPAMRELTTSLLL
                     YGPFTRTMGVAIYSINEEGYTVQACALAGVAIIIIVLGELLLRFVTRERRGQ"
     gene            2061..3167
                     /locus_tag="PLM74_10135"
     CDS             2061..3167
                     /locus_tag="PLM74_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007708476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON43465.1"
                     /translation="MDQVVLKNVTKSFVTKAFGKVTAVDGVSLSIREGECFSFLGPSG
                     CGKTTTLRMIAGFEDLSSGEIWLGDRPVSIKTKNFYVPPEKRQLGMVFQAFAVWPHLN
                     VFENVAFPLRIQRMSNAEISDRVKQALKHTSLSGLEEKYPSELSGGQQQRIALARAIV
                     ASARVLLLDEPLSNLDPKLREIMRFEIKDLQKKLGLTIVYVTHDQAEAMALSDRMLVM
                     DMGVVQQVDTPTSMYNNPANRFVFSFLGLSNFVPVALHDGLVWPAGSASSSLECEIPH
                     DWDDDRAVLATRPNEIQLTAEGGYRTRIKNRVFLGDYVEYRMDIGGTEIRVQTHEHRD
                     FRAGDVCGIRLGKAKWYPAEDEVSDEERERRKVI"
BASE COUNT          682 a          907 c         1057 g          758 t
ORIGIN      
        1 ataccctgtg ccttcgggca tgggcgacgg gcctttctac gctgcctgcc gcccaatgga
       61 catcaggctt tccagggagg tggctgatgc gggagtggaa gcgagaacgg gcgctggcgc
      121 cggtgctggc gcgggagggg cgctcaaggg cctggtcacg cgtgtgatct acctgggcaa
      181 catctacgag tatcgtgtgc aggtgggcga cagtgagatc cgggtgcagc aagactcgtt
      241 cgaggccttc aggcacggag tattgcgcga gggggaagtc tgcgcactca gcatcgataa
      301 tatccgctac tacgacgaca tggaggaggt gtcatagtga gagctggtca gtctccgaga
      361 ggcagcgagc tcaggcgcat ggaaggctca aggttcaaca ccggccagct gctgcttgtc
      421 atctccaccg taatactgat cattaccgtg gtcttgcctg tggcaatgat cgtgtacaac
      481 gtcttcttct acaaatggtc gttcgactgg agcctctttg cttcgatgct caccgatccg
      541 gataatctgg cggccatgtg gaacacggta aagatatcct tcttcgtcac caccttgggc
      601 actgttgttg gcctcttctt cgcgtggctg atcggtcgaa gcgacattcc gctcaagggg
      661 ctcatgaagt ctctcttcgt gattccgtac atgttcccgc cctttatcgg ggcgatggcc
      721 tgggggttgc tcttggcgcc gcgctcggga tacataaaca agatgttcat ggcgctgact
      781 ggcgggcgta cccctctgtt caacattaac acattggcag gcatcgtctt tgtagagctg
      841 tgctactact tcccgttcgt gttcattcag gtgtcggggg cgctggagcg gatggatccc
      901 actctggaag agtcggctag gatcgccggc gccaaccaga tgactgtgat acgcaggatc
      961 acaatgccgt tggtggttcc ggcgatcgct gcaggcgcta tgctgatcct catctcgtcg
     1021 ctgtcgcact tcggggttcc tgcgatcttg ggcttctcca acggcatctt cacactgccg
     1081 accaagatct acgagctgat ctaccgggca tcgggaagct tcgagggaat caggcgcgga
     1141 gcgtccctgt caattctgct ggtggccgtg gtcgtgctgg cgttggcatt gcagcgacgg
     1201 gtgctgcgag ccgggcgtta cgacataatc aggggcaaga gcatgaggcc catgctgatc
     1261 aagctgcgcg gatggaaggc tccgatgctt ttggtctgct tcgtgtttct ggccatcact
     1321 gtgatcctgc cgctattcac catcttcggc gtcggttttc tcaaggctta cggtgtgccc
     1381 atgaagcttg ccaacatgac tttgaacaac ttcaagtatg tgttgttcga gtcgagaatg
     1441 gcgcgtgact ccatacggaa cagcttcttc ctgtcgataa cgtcggcagt cataacgatg
     1501 ctggtgggga ccatgatcgc ctacgtaatc gtcaagatac agccgagggg caagggagta
     1561 ctcgaggtgc ttggcatact gccatactcc ataccgggaa ttgtgttggc ggtgggtgtc
     1621 atcttgacgt ggagcggggc gttctacctg aacctataca acacgatctg gattatcctg
     1681 atcgcgtata tcgcgcgcta catggcgttt tcgatgaagt cggcgtctgc gtcgctagaa
     1741 caggtgcacg attcgctgga agaagcctcg cgcacatgcg gcgcgagcca cttcgaatcg
     1801 cttctggatg tgacactgcc cctgatccga ccgggaatgg tcgcaggttt cttcctcata
     1861 ttcctgccgg ccatgaggga actcacgacc tcgctactgc tctacggacc cttcacgcgc
     1921 accatggggg tggccatcta ctccatcaat gaagagggat acacagtgca ggcgtgtgcc
     1981 ctggccggag tggccatcat catcattgtc ctcggcgaac tgctgctcag gttcgtcacg
     2041 cgggaaagga ggggacagtg atggaccagg tcgtgctgaa gaacgtcacg aaatccttcg
     2101 ttactaaggc cttcggcaag gtaactgcag ttgatggcgt gagcttgagc atccgcgaag
     2161 gcgagtgctt ctccttcctg gggccctcag gatgcggcaa gaccacgact ctcaggatga
     2221 tcgcggggtt tgaggacctg tcgtcaggag agatatggct gggcgacagg cccgtgtcga
     2281 tcaagacgaa gaacttctac gtgcctcctg agaagagaca gctcggtatg gtgttccagg
     2341 cgttcgctgt gtggccgcac cttaacgtgt tcgagaacgt ggccttccct ctccgcattc
     2401 aacggatgag caatgcggag atatcagacc gagtcaagca ggcactgaag cataccagcc
     2461 tatcggggct ggaggagaag tacccctcgg agctgtcagg cggtcagcag cagcggatcg
     2521 ctttggccag agccatcgtt gccagcgcta gagtgctgtt gctggatgaa cccctgtcca
     2581 atctcgaccc taagctccgc gagatcatgc gctttgagat caaggacctc cagaagaagc
     2641 tgggcttgac catcgtgtac gtcacgcatg atcaggcgga agcgatggcg ctgtccgaca
     2701 ggatgctcgt catggacatg ggcgttgtgc agcaggtgga tactccaact agcatgtaca
     2761 acaacccggc gaacaggttc gtattcagct tccttgggct atcgaacttc gtgcctgtgg
     2821 cgctgcacga tgggctggtt tggccggcgg gttcggcgag cagcagtctg gagtgtgaga
     2881 tcccgcatga ttgggacgat gacagagctg tgcttgccac tcgtccgaat gagattcagc
     2941 tcactgcgga aggcggttac cgtacgcgca taaagaaccg agtcttcctc ggtgactatg
     3001 tcgaataccg catggatata ggcggcaccg agatcagagt tcaaacccat gaacacaggg
     3061 atttcagggc gggagacgtg tgcggcatcc gactgggcaa ggccaaatgg taccccgccg
     3121 aagacgaagt ctccgacgag gaaagggaac ggcggaaggt aatctagaga aggcgatccg
     3181 ggatgcggta tcgtacgagc acctaagctg aggatgagca acgaggaccc gcgacctgca
     3241 ggtggaggcg cgcgggtcct tctcttcgtc cggcaggacg cgtgcggcgc ctagagaatg
     3301 aagcgcttgg atgatgtcta cttgtggagg tgacttccgg gtgaccacga ctctatgcat
     3361 tatcgcctac ctggcggtga tcgcgccgct gattgtgctc gtcc
//
LOCUS       DAOTAC010000268         3361 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_22415_length_3361_cov_4.777979, whole genome shotgun sequence.
ACCESSION   DAOTAC010000268 DAOTAC010000000
VERSION     DAOTAC010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22415_length_3361_cov_4.777979"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..987)
                     /locus_tag="PLM74_10140"
     CDS             complement(<1..987)
                     /locus_tag="PLM74_10140"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HON43466.1"
                     /translation="MHLRRMRTLLVALTAILLVFGASAFGGVQSEDAVLLTVSVIDVG
                     QGDSVLIATSDGHAMLVDGGPADASSKVINAISKAGCDKLDVLVSTHPHADHIGGLAR
                     VLDGVRVDRVIDSGKVHTSKTYEGYLEKILAKDIPFSLGRAGSSFALGPAAVTILWPV
                     EPLAENINDCSIVVRVTYGGFSALLVGDIEEPSEQALIKAGSLSPATALKVAHHGSDT
                     STTDAFLGIVRPAIAIISVGAGNGYGHPTASVLARLKTSGVKVYRTDLNGTVSVKTDG
                     KSWQVETERNDGSKAAPTPAPTPTSGTGQKYYGSRNSNVFHYAWCRYVSNIKA"
     gene            complement(1382..1975)
                     /locus_tag="PLM74_10145"
     CDS             complement(1382..1975)
                     /locus_tag="PLM74_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43467.1"
                     /translation="MPREVKWSIVTLVILLALSCMAIAGEPVKAYRNLVFGESRRETD
                     RKIVADKALQGNSYTCFATLAGNRYGLECGFDQDRLFLIKFTANFTFTDRIYSASQFD
                     KTLMEVETLRSIIETQYGPPHGVEDVSLLSAKDGKVAFRYYWEVDSVKAIMIGIAARQ
                     FKYWVEMSICHIPIMAEISARLAQQESTAVMDSAGDF"
     gene            complement(2569..>2988)
                     /locus_tag="PLM74_10150"
     CDS             complement(2569..>2988)
                     /locus_tag="PLM74_10150"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HON43468.1"
                     /translation="TLRRSKTDQDGQGQVIAVPYGRYETTCPVLAVKTWLDASGITAG
                     PVFRAVDRHGNIAAARLSDRAVANIVKRAAAAAGLDPADYAGHSLRSGMATAAARAGA
                     SERAIMNQTRHRSVAMVRRYIHRGQMFADNAAGRLGL"
     assembly_gap    2989..2998
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2999..>3361)
                     /locus_tag="PLM74_10155"
     CDS             complement(<2999..>3361)
                     /locus_tag="PLM74_10155"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HON43469.1"
                     /translation="AAWCDTQGLRAMPAAPETVTAYITALADQGLAAGTIGRRLTAIG
                     QAHANQSQQFDAKHPLIRATLSGIRRTLGTAQAPKQALLTEDVRAMVLALPEGLLGLR
                     DRALLLVGFAAALRRSELV"
BASE COUNT          627 a         1009 c          983 g          732 t
ORIGIN      
        1 ggccttgatg ttggagacat acctgcacca tgcgtagtgg aacacgttcg agttcctgct
       61 tccgtagtac ttctggcccg tcccgctggt cggcgttggc gctggggtgg gagcggcctt
      121 gctcccatcg tttcgctcgg tctcgacttg ccagctcttc ccgtcggtct ttacgctgac
      181 ggtgccattc aggtcagtcc ggtaaacctt cacccctgag gtcttgagcc ttgcaagaac
      241 gctagcggtc ggatggccgt agccgttgcc cgcgcccacc gagatgatgg ctatggcggg
      301 cctcacgatg ccgagaaacg cgtcagttgt ggaagtgtcg cttccatgat gggcaacctt
      361 gagcgccgtg gcgggcgaaa gcgatcctgc cttgatcagc gcctgctcgg aaggctcttc
      421 aatgtcgccg acaaggagcg ccgagaaccc gccataggta acccggacca caatggagca
      481 atcattgatg ttctccgcta ggggttccac cggccacagg atcgtgaccg cggcggggcc
      541 cagggcgaac gacgatcccg cccggcccag agagaagggg atatccttcg cgaggatttt
      601 ctccagatac ccctcgtagg tcttggaggt gtggaccttg cctgagtcga tgaccctatc
      661 cacgcgtacc ccgtcgagca cgcgggcgag gccgccgatg tgatcggcat gcggatgtgt
      721 gctcacgaga acgtccagct tatcgcagcc ggccttactg atcgcgttga tgacctttga
      781 cgaggcgtcc gccgggccac cgtcgactag catggcgtgc ccgtcggatg tcgctatcag
      841 aaccgagtct ccctggccga cgtctatcac cgaaacggtg agcaggacgg catcctcaga
      901 ctgcacgccg ccgaaagcgc tagctccaaa gacaagcaga attgcggtca gagcaacgag
      961 cagcgttcgc attctcctca gatgcatgtt gtcttccctc catctctatc cgaccatata
     1021 gagcttatcg acggcgctac atggccaaga acgattgccg agactcgcca ccttatctgt
     1081 ttagctgctt cgccattcga ctggcggcca cggcggccat gtagcagtcc ttcttcacag
     1141 cctcaactgc ccagccgcgt tgtcggccaa catctgccta cggcgaacgt gcctccgcgc
     1201 cgtagtgacg gaataatggt ccgtctggtc cataggacac tgttctgcag aatcgcaggg
     1261 taaggtgttc agcattttgc ccagggtggg ctggacccga tacgagcctt tgccacgttt
     1321 gtaaacggca ccacccagac gggcgaggat ccgggttaag ctcacgatac tgtgtccttc
     1381 cttagaaatc acctgcgctg tccattacag cagtgctctc ctgctgggca agcctggctg
     1441 agatttctgc catgatcggg atatggcaga tggacatctc cacccagtac ttgaactgtc
     1501 tcgccgcaat accaatcatg atggccttca cgctgtcaac ttcccagtaa taccggaagg
     1561 ctaccttacc atcctttgcg ctgagtaaac tgacatcctc cacaccatgc ggaggaccat
     1621 actgggtttc aattattgag cgtagagtct ccacttccat tagagtcttg tcgaactggc
     1681 tggccgaata gatacggtcg gtgaatgtga agttcgcggt gaatttgata aggaacagcc
     1741 ggtcctggtc gaatccgcat tcgaggccat atcgattccc ggctagtgtc gcaaagcaag
     1801 tgtagctgtt cccctgcagc gctttatcag caacgatctt ccggtctgtt tcacgccggg
     1861 actctccaaa aacgagattc ctgtaggctt tcacaggttc gccggctata gccatgcaag
     1921 agagcgccag caagatcact aacgtaacga tgctccactt cacttctcta ggcattgata
     1981 cccctcccat tcatcccaga cctactcata gtgctggtga gcgtctggag agagtcgtct
     2041 tctttgtccg cccatcctct tgacgcccga gtctgcgcac ccagaagtgc ctgagggaac
     2101 tcatcagctt cacgctcatg ccacccaggg ggaatcagcg taccttcggc aaccaaatag
     2161 tatccggcgc agccagggag aagcgcatgt cctggttcat ggacgcgtgt gtatgagcgg
     2221 gtcttcatcg agtgtggagt gcatcgatca tgttgcggat tattggtgtg acatgtagac
     2281 gtgagcctgc gggcgggatg ctgacagcga ttcctggcat cgtccgcgga tttgctgtgc
     2341 aaggcgattt cccactgcag atgtgtattc gtccggattt tctggatccc ttcatcattt
     2401 tccatgatcg gcgttttccg attctccgga cagcataacg gcagcagccc gccgatattg
     2461 catgaagtgc gcggggggct aggcaatcgg tgttgcctct cggtgagcgt ggagggtaat
     2521 cggattcaac tacgttgata tctattcctt gcttgccctg gcccttccct acagccccaa
     2581 ccgccctgca gcgttgtcgg cgaacatctg tccacggtgg atatacctcc gcaccatagc
     2641 cactgagcgg tgtctggtct ggttcatgat ggccctttca gaagcgccgg ccctggcggc
     2701 cgcggtagcc atgcccgaac gcagagagtg cccggcatag tcggcaggat cgaggcccgc
     2761 ggccgcggcg gcccgcttga cgatgttggc gacggccctg tcgctgagcc ttgcggcggc
     2821 gatgttcccg tggcggtcca cggcccggaa cacgggcccg gcggttatcc cagacgcgtc
     2881 gagccatgtc tttacagcga gtacgggaca tgtcgtctca tagcgtccat aaggaacggc
     2941 gatgacttgt ccctgcccgt cctggtcggt cttggagcgg cggagggtnn nnnnnnnnac
     3001 tagctcggag cgcctgagag ccgccgcgaa gcctacgagg agcagggccc tgtcgcgcag
     3061 tcccaggagg ccttcgggca gcgccagcac catcgccctg acatcctcag tcagcaaagc
     3121 ctgcttcggc gcctgtgccg tgcccagggt gcggcgtatg ccggacaacg tggccctgat
     3181 caacgggtgc ttcgcatcga actgctgact ctgattggcg tgagcctggc cgatggcggt
     3241 gagccggcgt cctatggtgc cggctgcaag cccttggtcg gcgagggctg ttatgtatgc
     3301 tgtgaccgtc tcaggcgcgg caggcatggc ccggaggcct tgcgtgtcgc accaggcggc
     3361 g
//
LOCUS       DAOTAC010000269         3351 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_22503_length_3351_cov_3.862864, whole genome shotgun sequence.
ACCESSION   DAOTAC010000269 DAOTAC010000000
VERSION     DAOTAC010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22503_length_3351_cov_3.862864"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="PLM74_10160"
     CDS             <1..670
                     /locus_tag="PLM74_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43470.1"
                     /translation="AKLAYNTKLLDADLTLRSGIKQANTVKPAASVATTAQLGFDIAY
                     KPIEAGFTWERGIESKKVGYIGYAKANIPFGDALTIKGDWEQRFATDEYYTYNAGLVL
                     SLPVVPDKVTLGAEAAYGKTWHDTLDAAKDYAKWLMKGDVAWQINPATKATGTASYEW
                     REYEASSKPSGEYLQLGLAVGHKLFDATSLNFKYDLKDVYYSATGFNDYTVRILELSL
                     KTEF"
     gene            682..1161
                     /locus_tag="PLM74_10165"
     CDS             682..1161
                     /locus_tag="PLM74_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pre-16S rRNA-processing nuclease YqgF"
                     /protein_id="HON43471.1"
                     /translation="MRPGKHCFPGRFSREEVSPLDEIVLAVDPGRDKAGMAVVSKTRG
                     TLFRCIVSADACASRGTELARAYGAHKIVVGDRTGSREVGRALALSSGLRVTTVDEHR
                     SSMEGRQRYLLEHRGRGLCRFVPVGLRTPPEPFDDYVAEILAARYFEQQCREKESTS"
     gene            1211..2689
                     /locus_tag="PLM74_10170"
     CDS             1211..2689
                     /locus_tag="PLM74_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43472.1"
                     /translation="MRLASARTRRCCMLMLAVLCAALVGALSPSAHAAPLITPDDWRY
                     TALVELGRAGLLDERDAGVYASGVPVSLGDLSLTVERTIRHLAGASVGGEISVSGSVA
                     LSPGPLRLTGPIPTNLTISATQANLIARLVDEFGPTFSDQSLANLSLGVPFRSPGGGM
                     VLSLSESALPLPSHRLLSDIGAYASVSTAPASMRPPASQPELVRLQDLLTPSVRLGSE
                     DAEPRFQAGVPSPLGTLAVEMSSQAARRPGKDDTGEERQPTRGFDLSTSVRLADILKV
                     SADFFTDDKSPQKPTSTALGVVLGETEGAGLSVGHRVTDMGAGAANTGGLRETVTSVD
                     VKYSLPDVVGRAVSAGSTDSITVRAGYELYGREQPLLAAKSSLQATASVVIDYKLLLG
                     DAAFLQAGYRYERIRDLIASGAVWTKSEMYGDGGLSAWPGIEPLKLDGSDATRTVTSI
                     DFGYRLMGDTSLLLGYKLIDFSDIGALDFTKNLATAEVTIRF"
     gene            2726..>3351
                     /locus_tag="PLM74_10175"
     CDS             2726..>3351
                     /locus_tag="PLM74_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43473.1"
                     /translation="MRRSANRAAAMLVAIVLLYAVGHAAAQSSTTSPVERLVAMEGLL
                     FGSQQQGAIVARLQNIENVVFGEPGSGTLPERLDQCWNFVLGDRAGGMNLKFKLKAVQ
                     WTIFGKITDGPIVPMLVDLEGSVIGRPGTGPVGPRLDILMRLSIAGGRPEVGYSLVPK
                     GSLVKVRLLTPLSSVKARSGDVVKFEVVDNVIFEDRLVIPKGTPLTARV"
BASE COUNT          661 a          928 c         1090 g          672 t
ORIGIN      
        1 cgcgaagctg gcatacaaca ccaagctgct cgatgcggat ctgacgctaa ggagcggcat
       61 caagcaggca aacaccgtaa agccggcagc tagtgtcgcc actactgcgc aactcggctt
      121 cgacatagcc tacaagccga tcgaggccgg gttcacctgg gagcgcggga tcgagagcaa
      181 gaaggtaggc tacatcggct acgcaaaggc caacatcccg ttcggcgatg ctctgaccat
      241 caagggtgat tgggagcaga ggttcgccac ggatgagtac tacacgtaca acgcgggtct
      301 ggtcctctca ttgccagtgg tacccgacaa ggtgacacta ggcgctgagg cagcctacgg
      361 gaagacctgg cacgacactc tagacgcggc caaggactac gccaagtggc tcatgaaggg
      421 cgacgtggcg tggcagatta acccggcgac caaggcaacc ggtacggcgt cgtatgagtg
      481 gcgcgagtac gaggcgtcaa gtaagccttc cggcgagtac ctgcagttgg gtctggcagt
      541 cggccataag ctgttcgatg ccaccagcct gaacttcaag tacgacctga aggatgtcta
      601 ctactcggcg actgggttca acgactacac tgtgcgtatt ctcgagctgt cgctcaagac
      661 cgagttctag gttgatgcga gatgcgcccc gggaagcact gcttcccggg gcgtttttct
      721 agagaagagg tgagtccgct ggacgagatt gtgctggcag tagaccctgg gcgcgacaag
      781 gcaggcatgg ccgtagtgtc gaagacacgt ggcacgttgt tccgctgcat tgtgagcgcg
      841 gacgcctgcg cgtccagagg aactgagctc gccagggcat acggcgcgca taagatcgtg
      901 gttggtgacc gaaccggctc tcgcgaggtc gggcgggcgt tggcgctctc gtcggggctt
      961 agggtaacca ctgtcgatga acacagatca tcgatggaag gaaggcaacg atacctcctg
     1021 gagcatcgcg ggagagggct ctgcaggttc gtgccggtcg gcctcaggac tccgccggaa
     1081 ccgtttgacg actacgttgc agagatcctc gctgccaggt attttgagca acagtgtagg
     1141 gagaaggaat caacaagttg acgaagaata tataaaagct gagtaatcgg cgggaaaggg
     1201 ggagcgtggc atgaggcttg cgtctgctcg cacacggcga tgctgcatgt tgatgctggc
     1261 ggtcttgtgc gcagcgctgg tgggagcatt gtcgccgtcc gcccatgccg ccccgctgat
     1321 cactccggac gattggagat acacagccct tgttgagctg ggccgagcag gcctgctgga
     1381 tgagcgcgac gccggggtct acgccagcgg cgtgccggtg tcccttggcg atctctcgct
     1441 caccgttgaa cggacgatca ggcatcttgc aggcgcttca gtgggcggcg agatctcggt
     1501 gtcggggagc gtggcactat cgcccggccc gctgaggctc acgggcccaa tacctacgaa
     1561 cctcactatc tctgccacgc aggccaacct catagctcgg ctcgtcgatg agttcgggcc
     1621 gaccttttct gatcaatcgc tcgccaacct ttcgctgggt gtgccctttc gcagccctgg
     1681 cggaggcatg gtacttagcc tctcagagtc ggcgcttcct ttgcccagcc atcgactgct
     1741 cagcgatatc ggagcttacg cgtccgtgtc gactgcaccc gcttcgatgc gtcctccggc
     1801 tagccagcca gagctagtaa ggctccagga tctgctgacg ccgtctgtga ggctcggctc
     1861 tgaggacgct gagccgcggt ttcaggccgg ggtgccaagt cccctgggca ctctagctgt
     1921 tgagatgtcc agtcaggccg cgcgacgccc gggcaaggac gatacgggtg aggagaggca
     1981 accgacgcgt ggcttcgacc tgtccacaag tgtgcgtttg gccgatatcc tcaaggtgag
     2041 tgcggacttc ttcaccgatg ataagtcgcc gcagaagccg acctccactg ccctgggagt
     2101 cgtgctaggt gagactgagg gagcaggcct cagcgtgggg caccgtgtga cggatatggg
     2161 cgccggtgcg gcgaacacgg gcggcctccg ggagactgtc acgtcggttg acgtgaagta
     2221 ttcgcttccc gacgttgtcg ggcgggcggt gtccgccggt tctacggact ccatcactgt
     2281 aagagccggc tatgagctgt atggaagaga acagccgtta ctggccgcga agagcagtct
     2341 tcaggccacg gcgtcggtgg taatcgatta caagctcctg ctcggtgatg ccgcgtttct
     2401 tcaggcgggc tataggtacg agaggattcg cgacctgatc gcttcgggcg ctgtgtggac
     2461 caagtccgag atgtacggcg acggaggcct gtcggcgtgg cccggaatcg agccgcttaa
     2521 gctcgacggt tcagacgcga ctcgcacggt tacctctata gatttcgggt acagactaat
     2581 gggcgacacc tcattgctgc tagggtataa gctgatcgac ttctccgata tcggggctct
     2641 agactttacc aagaatctcg ctaccgccga ggttaccatc aggttctagt ggtttcatcg
     2701 tgcgtcgcta cagggaggta gaaaaatgag aagatctgcg aacagggcag cggctatgct
     2761 cgtcgcaatt gtgctgttgt acgctgtcgg ccatgccgcg gcgcaatcct caactactag
     2821 cccggttgag cgcctagtcg ctatggaggg gctgcttttc ggttcacagc agcagggagc
     2881 tattgtggca cgcctccaga acatagaaaa cgttgtgttc ggcgaacccg gatcgggcac
     2941 acttcctgag aggctcgatc aatgctggaa cttcgttttg ggtgaccgcg ccggcggcat
     3001 gaacctcaag ttcaagctga aggcggttca gtggacgatt ttcggcaaga ttacggacgg
     3061 gcccatagtg cccatgcttg tcgacctgga aggcagcgtc atcgggcggc ctggcaccgg
     3121 gccggtcggg ccaaggctcg acattttgat gaggctatcg atcgccggag gaaggccgga
     3181 ggtgggatac tccctcgttc ccaaggggtc gttggtcaag gtaaggcttt tgacgcctct
     3241 gagctcagtc aaggctcgct ccggcgatgt tgtgaagttt gaagtggtcg acaacgtcat
     3301 cttcgaagat cggctggtca tcccgaaagg gacgcccctt acggcgcggg t
//
LOCUS       DAOTAC010000270         3323 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_22727_length_3323_cov_5.959302, whole genome shotgun sequence.
ACCESSION   DAOTAC010000270 DAOTAC010000000
VERSION     DAOTAC010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22727_length_3323_cov_5.959302"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            123..617
                     /locus_tag="PLM74_10180"
     CDS             123..617
                     /locus_tag="PLM74_10180"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HON43474.1"
                     /translation="MQINFDESVPIYVQIMQAVMRDVAVGELAPGDKLLSVRDLARDA
                     KTNPNTVQRAYMELERIGIVETQRGQGTFIRATPGLAKEVRSEVTATAVRSFINEMRR
                     LRYADDEIVALVSQGLNQASAAGDSSADCSDADGRDQASRRQSTYDQPIAGRSATDPD
                     GGVH"
     gene            617..1309
                     /locus_tag="PLM74_10185"
     CDS             617..1309
                     /locus_tag="PLM74_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON43475.1"
                     /translation="MLAIEMANVQKRYYRSHALRGVNLQIEPGKIVGLLGPNGAGKST
                     LLKICAGLTHPTAGRVHVLGRDVSTATKALTAFVPENDTFYSWMTARQAVAFESSMFS
                     DMDVSDAMDLLRGLRLDPDNKISSLSKGQRGRLKLALAMSRRAGLVLMDEPLAGIDPP
                     SRAAILETIASRYRAGEQTIIISTHEVLESEKLFEDVVFLSDGQIALAGNADELRAER
                     GKSLNEIFAEVC"
     gene            1311..2096
                     /locus_tag="PLM74_10190"
     CDS             1311..2096
                     /locus_tag="PLM74_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43476.1"
                     /translation="MKRWWSMVGKEWRGTWTLLAVAIAAVIALDVWLGIKTATWSLGH
                     MGEGIAVGLSSIPFAGVGIATVVAGYFAMRGEWQSRTSMRTLSYPISGTGLLTARLAV
                     IAFNLVLVTIVAATGVWFVANRTPGFWMMMTGDPAFMTWVRSREFLTSAIWLTISAIL
                     GIAFMVAVGMFSFIVGTLVPRVSGLIAVAVHVLVWYLCFAFGPHAMYIASFLPNPVIV
                     GFDNPLGLVRRIAVPLLPFWPTLAITAVLLIVAGRILESEVDA"
     gene            2111..3040
                     /locus_tag="PLM74_10195"
     CDS             2111..3040
                     /locus_tag="PLM74_10195"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HON43477.1"
                     /translation="MYSRRSIVLAVLVGLAFLASTTQPWIQGSRLLANKYADEFSFSG
                     FVYEGSSKAVDDSFHKEFGAAGIRSVQVEARPGDITVTRADDDMISINGERVAYGPSN
                     QIAVERLHYVCLDAVQDGDVVRVVGSIWHPEPSSPNPAGPNGGFTNIVLRVPPGLAIY
                     LQTPAGKVALDGLTGTVEAHVDMGSIEARSITGNLILGTSAGRIAVTNATIEEELSLT
                     TSMGDIEFDGSLGARNHVEVSAGRAQMTVPRSTCVRLDAATNAGNVDIRLPVTGTKSE
                     AAKSSAVGILGEGQPTGDLKIRVSMGEISISGR"
BASE COUNT          716 a          954 c         1026 g          627 t
ORIGIN      
        1 cgcagcgccc acaatcgcgc ctcagcgtcg ccacacatcg cggcccagcg cagcggagac
       61 cgggcggcgc tccggaagtt gaccgaatcg tatgccagat gtcacaggaa ggaggaggct
      121 gagtgcagat caatttcgac gagtcagtcc ccatctacgt gcagataatg caggccgtca
      181 tgcgggatgt ggcagtgggt gaactggcgc ccggcgacaa gctactgtca gtcagggatc
      241 tggccaggga cgccaagacc aaccccaaca cggtgcaacg cgcctacatg gaacttgaga
      301 gaatcggcat tgtcgaaacc caaaggggcc agggaacatt catcagggct accccagggc
      361 tagcaaagga ggtcagatcc gaggtgacag caaccgcagt gcggtcattc ataaacgaga
      421 tgagacggtt acgctacgct gatgacgaga tcgtcgctct ggtgtcgcag ggccttaacc
      481 aggcgtcggc tgcaggcgac agcagtgccg actgcagcga cgcagacggc cgagaccagg
      541 ctagtcgacg ccagagcaca tatgaccagc cgatcgcagg ccggtcggcc acggatccgg
      601 acggaggtgt tcactgatgc tagccattga aatggccaat gtccagaaga gatactatcg
      661 ttctcacgcg cttcggggcg tgaatctcca gatagagccg ggcaagatcg taggcctgct
      721 cgggccgaac ggggccggga agtctacgtt gcttaagata tgcgcagggc taacccatcc
      781 gacggcagga agagtgcacg tactcgggcg agacgtatcc acggcgacca aagccctcac
      841 cgcattcgtg cctgaaaacg acactttcta ctcctggatg accgcgcggc aagccgtcgc
      901 tttcgaaagc tcgatgttca gcgatatgga cgtatccgat gctatggatc tacttagggg
      961 gctgagactg gacccggaca acaagatctc ctcgctttcc aaggggcagc gtggtcgtct
     1021 taagctggcg ctggcaatgt cgcgccgggc aggactggtg ctcatggacg aaccgctcgc
     1081 aggcatcgac ccaccgtcgc gcgccgctat cctggagacc atagcctcga gataccgcgc
     1141 gggcgagcag accataatca tatccaccca cgaggtgctg gaatcggaga agttgttcga
     1201 agacgtcgtg ttcctgtcgg acggacagat tgcgcttgca ggcaatgccg acgaactccg
     1261 agccgaacgg ggcaaatcgc taaatgagat cttcgcggag gtgtgctgag gtgaagcgtt
     1321 ggtggagtat ggtaggcaag gaatggcgcg gcacatggac gctgcttgca gtagctatag
     1381 cagccgttat agcgctcgat gtgtggctcg ggatcaagac cgcaacgtgg tcgctcgggc
     1441 acatgggcga aggaatcgca gtgggtctgt catcgatccc gtttgcgggt gtagggatcg
     1501 ctaccgtagt ggccggctac ttcgccatgc gaggcgagtg gcagtcgcgc acctcgatgc
     1561 ggacgctatc ctacccgata agcgggacag gattgctcac ggcaaggctg gccgtgatcg
     1621 cattcaacct ggtgcttgtc acaatcgtgg cggccaccgg cgtctggttc gtcgcgaata
     1681 ggacgcccgg attctggatg atgatgacag gcgatcctgc cttcatgacc tgggttaggt
     1741 ctcgcgagtt tctgacgagc gcgatctggc tcacgatatc cgccattcta ggtatcgcct
     1801 tcatggtagc tgtggggatg ttctcgttca tcgttggtac tctggtgccc cgggtttccg
     1861 gactgatagc agtggccgtg catgtgctag tgtggtatct ctgcttcgca ttcgggccgc
     1921 acgcaatgta catcgcctcg ttcctcccca acccggtgat tgtgggattc gacaaccctc
     1981 tgggtctggt gaggcgaatc gcagtgccac tgctcccgtt ttggcctaca cttgcaataa
     2041 cggcagtcct gctcatcgtt gcaggcagaa tccttgagtc ggaagtcgac gcgtaaggag
     2101 ggacagatga atgtactcca gaagaagcat agttctcgcg gttctcgtgg gcctggcatt
     2161 cctggccagc acaacgcagc catggataca gggcagcaga ctgcttgcca acaagtacgc
     2221 tgatgagttc tccttctccg gttttgtgta tgagggttcg tccaaagcgg tggacgattc
     2281 tttccataag gaattcggcg ctgccgggat tcgaagcgtt caggttgagg cacgacctgg
     2341 cgatatcacc gtgacgcggg ccgatgacga tatgatcagc atcaacggtg agcgggtcgc
     2401 gtacggcccc tcaaaccaga tcgccgtgga gcgcctgcac tacgtgtgcc tggacgctgt
     2461 gcaggatgga gatgttgttc gggtcgtcgg ctcgatatgg catccggagc cttcatcgcc
     2521 gaacccggca ggcccgaatg ggggattcac gaacatagtg ctcagggtgc cgccgggtct
     2581 agccatctac ctacagaccc cagcgggcaa ggtcgcactc gacggcttga cgggaacggt
     2641 cgaagcccac gtcgacatgg gtagcataga agcccggtct atcactggaa acctcatact
     2701 cggaacctcc gccggacgca tcgcagtgac caatgcgacc atcgaagaag agttgtcgct
     2761 gaccacgtcg atgggagata tcgaattcga cggatcgctc ggcgctcgaa accatgtaga
     2821 agtttcagcc ggcagagcgc agatgactgt cccccgatcc acctgtgtca ggctagacgc
     2881 cgctaccaac gcaggaaacg tagacatcag actgcctgtt accgggacaa agtcagaagc
     2941 agcgaagtcg tccgcagtcg gcatccttgg cgaaggacaa ccaaccggcg atctgaagat
     3001 ccgagtgtcg atgggcgaga tcagcatctc gggcagatga ctctgcatag cacacacggc
     3061 agcatgccag aagcagaaaa ggccggacgt cgcacgctgc ccacgtcggc agtcgcaccg
     3121 tccggccatt tgccctggca aacagctgtc tacccgcagc tagtagccct ccgccagtag
     3181 cacccgctgg gagctagcgg cggccaacag cgactgacag ttccctactc caagccgggg
     3241 tacaccatcg gcacttgggc aagcctttcg gccttcgcat cgtaagctcc gagtatctcg
     3301 gcctgcgccc gccccgcggg aac
//
LOCUS       DAOTAC010000271         3318 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_22767_length_3318_cov_6.953724, whole genome shotgun sequence.
ACCESSION   DAOTAC010000271 DAOTAC010000000
VERSION     DAOTAC010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22767_length_3318_cov_6.953724"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..938
                     /locus_tag="PLM74_10200"
     CDS             <1..938
                     /locus_tag="PLM74_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43478.1"
                     /translation="DVTPLHNKLIEAMLAAKLHLIVTMRSKMEYVQDKDDKGRTVIRK
                     VGMQPVQRDGLEYEFDVVADMDTDNNFVVGKTRCPALAGAVVARPGKEVADTLVAWLT
                     DGAPPAQPLPSAPRPASAPAPQQRPAAPATAQPAPAPQRQPQTQPAAPRQAQPASQSG
                     NGQGNGQVGDPRAISIRFGKYAGMTLGQIAATDMGYAKWLADNAQQTDVREAAKAIIA
                     ATPQAQPTQQGPTQQQIARLMAIAHDGNIAKETIQAYMQQHYGIDSLKQLTREQYDEV
                     CEWLQNGGLDPDDIPPEAYDDQQATFGDVAGTPQW"
     gene            1180..1434
                     /locus_tag="PLM74_10205"
     CDS             1180..1434
                     /locus_tag="PLM74_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HON43479.1"
                     /translation="MAVKATGYVMPIDHLGRVVIPKKVRDPRIALPSAAFEIHVDGDK
                     IILEPYRAPCVICGSTSDEAAMIRHRRVCRQCTALARLVG"
     gene            1447..1731
                     /locus_tag="PLM74_10210"
     CDS             1447..1731
                     /locus_tag="PLM74_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43480.1"
                     /translation="MRTLTRALAAMLTDPPLRPAVRHLEARRAVFNETENLDAQDAAY
                     MEVLAAQMELRAEIHRARLALAAKIEAVNPDEAQRIRESLTGDEGGGGAQ"
     gene            1728..2687
                     /locus_tag="PLM74_10215"
     CDS             1728..2687
                     /locus_tag="PLM74_10215"
                     /inference="COORDINATES: protein motif:HMM:TIGR01446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HON43481.1"
                     /translation="MSRYATIYTKIWQDETFKTLDSDAQMLFIYLLTSPHSNLIGFYY
                     LPMAYVAEDLGNPCETLSKPFRNLCEKGLIEYDSAAKVVLVRKYLKYNPIQNPNQAKG
                     AVAVLESMPRTRLLAAFLECVEKYCPNHFETLRKPFRKGFETVSKPESESDTDTESES
                     SARAREADDDVVDNSDGDSEVYDPAIKQLVNFYFETFGKIPSPLILDDLSDWADKLGP
                     DLVCEAMRRTAAANSRSWKYSEAILRNWRDSRVRTMADVERLDREHQARTNRRAPPAL
                     MPEEPEPVPVRVDPEEVARSQAELRRVMEELMPKELMPDASGA"
     gene            2671..3201
                     /locus_tag="PLM74_10220"
     CDS             2671..3201
                     /locus_tag="PLM74_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43482.1"
                     /translation="MPAEREKRVDLSEVPMRELRVKLWPILVGYERMSWPDSELTARL
                     GDFEIALWDHVRCSNCTATDIVTLSNGLVVVRDCKSSIGNGFYLGLHRKACMEYGIPV
                     FGAIGCGGPVAWAQRQTARATRGSNWIPADWDAPPAARLEARRPAKAQALPGLEMVGA
                     ALRRWQEGQVDKEVVS"
BASE COUNT          735 a          968 c         1081 g          534 t
ORIGIN      
        1 gggacgtgac gcctctccac aacaagctca tcgaggccat gctggccgcc aagctgcacc
       61 ttatcgtgac catgcgctcg aagatggaat acgtgcagga caaggacgat aagggccgca
      121 cggtgatccg caaggtaggc atgcagccgg tgcagcgcga cgggctcgaa tacgagttcg
      181 acgtggtggc cgacatggac acggacaata acttcgtggt gggcaagacc cgctgcccgg
      241 cactggccgg tgctgttgtg gccaggccag gcaaggaggt cgccgatact ctcgtggcgt
      301 ggctcacgga cggtgctccg ccggcgcaac cgttgccttc ggctccccgg cccgcgtcgg
      361 ctccagcacc ccaacagcga cccgctgcgc cggcaaccgc gcaaccggca cccgcacctc
      421 agaggcagcc gcagacccag cctgccgcac ctcgacaggc gcagcctgcc agccagtccg
      481 gcaacggtca gggtaacggt caggtgggcg atcctcgcgc cataagtatc agattcggca
      541 agtatgcggg catgaccctc ggccagatcg ccgcaaccga catgggctac gccaagtggc
      601 tggctgacaa cgcacagcag acggatgtcc gtgaggccgc caaggccatc atcgcggcta
      661 caccacaggc tcagcccacg cagcaggggc cgacgcagca gcagatcgcc cggctgatgg
      721 ccatcgcgca cgatggcaac atcgccaagg agaccatcca ggcatacatg cagcagcact
      781 acggcatcga ctcgctcaag cagctcaccc gcgagcagta tgacgaggtg tgcgagtggc
      841 tacagaacgg tggactcgat cctgacgaca tcccgcctga ggcatatgac gatcagcagg
      901 cgaccttcgg cgacgtggcc gggacgccgc agtggtagac agacccttgt ctggcggcgc
      961 ccaggccgtc gttgggagcc tggctcccgt gagagagcgc ccgcgccggg cccgcgccta
     1021 tgaataacga tacatggcgc gaataaacgc cagatagagt atgacgtccg ccggcggcgc
     1081 acggcgagaa taacccaacc cgagcgaaaa ctgctccgcc aagggcagtc attcacggcc
     1141 cgcgcgccgg cgggccaccg aaaacgagag gtgagactga tggcggtcaa agccactgga
     1201 tatgtcatgc caatagatca cttggggcgg gtagtaatcc cgaagaaggt ccgggatccg
     1261 cgcatcgcct tgccgagcgc agcgttcgag atccatgtcg acggcgacaa gatcatactg
     1321 gagccctatc gcgccccgtg cgtgatctgc ggatcgacga gcgacgaggc agcgatgatc
     1381 agacaccgcc gggtgtgcag acagtgcacg gctctggccc ggctggtggg gtaggaggcg
     1441 gcagagatga ggacgttgac ccgggctttg gcggccatgc tgaccgaccc gcccctgcga
     1501 ccggcggtgc ggcatctgga ggcgcggcgg gcggtattca acgagactga gaacctcgac
     1561 gcccaggacg cagcctacat ggaggtactg gctgcacaga tggagctgcg ggctgagata
     1621 cacagggccc ggctggcgct ggcggcgaag atcgaggcgg taaatcctga cgaagcgcag
     1681 cggatacggg agagcctgac cggagatgaa gggggcgggg gggcgcaatg agtaggtacg
     1741 cgacgatcta tacaaagatt tggcaggacg aaacattcaa aaccttagat tccgatgccc
     1801 agatgttgtt catatatctg ctgacatctc cgcacagcaa cctgatcggc ttctactact
     1861 tgcccatggc atatgtcgca gaagaccttg gcaacccttg cgaaaccctt tcgaaaccgt
     1921 ttcgaaacct ttgcgagaag ggcttaattg agtacgattc ggcagcgaaa gtcgtcctcg
     1981 tccgaaaata tctgaaatac aacccgatac aaaaccccaa tcaggcgaaa ggtgccgtgg
     2041 cggtgcttga gagcatgccc cgaacccgcc tcctagctgc gtttctggag tgtgtggaga
     2101 aatattgccc caaccacttc gaaacccttc ggaaaccctt tcgaaagggt ttcgaaacgg
     2161 tttcgaaacc agaatcagaa tcagatacag atacagaatc agagtcatcc gcgcgcgcgc
     2221 gcgaggcgga cgatgatgtt gtggataact ccgacgggga tagcgaggtt tatgaccctg
     2281 ccatcaaaca actcgtgaac ttctatttcg aaacattcgg caaaatcccg tcgccactga
     2341 ttctggacga cctcagcgat tgggcggaca agcttggccc agatctggtg tgcgaggcca
     2401 tgcggaggac ggcggccgcc aattcgcgga gctggaaata ctccgaggcc atcttgcgga
     2461 actggcggga tagccgtgtg cgcaccatgg ccgatgtgga gcgcttggac cgggaacacc
     2521 aggcgcggac gaaccgacgt gccccgccgg cgctgatgcc agaggaaccg gaacctgtgc
     2581 cggtgcgggt ggatcccgag gaggttgccc gtagccaggc ggagctaaga cgggtcatgg
     2641 aagagctgat gccaaaggag ttgatgccag atgccagcgg agcgtgagaa acgggtagat
     2701 ctgtcggaag tgccgatgcg agagctgcgc gtcaaactgt ggcctatcct cgtgggttat
     2761 gagcgcatga gctggccgga ctccgaactc acagcacgcc tgggtgattt tgagatagcc
     2821 ctgtgggacc atgtccgatg tagcaactgc acggccacag acatcgtgac gctttccaat
     2881 gggctggttg tcgtccgcga ctgcaagagc agtattggca atgggtttta ccttggcctg
     2941 caccgcaagg cctgtatgga gtatggcata cccgtattcg gcgcgatcgg atgtgggggc
     3001 ccggtggcat gggcacagcg ccagacagca cgcgcaactc gcgggagcaa ctggatcccc
     3061 gcggactggg atgcaccacc ggcagcgcga ttggaggcac gtaggccggc aaaggctcag
     3121 gcattgcccg gcctggagat ggtaggcgcg gccctacggc gatggcagga gggccaggta
     3181 gataaggagg tggtgtcatg aactactcgc tggcagacgg aagggagttg ttcgtgaccg
     3241 acgcgtttag caagggtcgg acgtggttca cggcctacaa ggcccctacg ggctcgacga
     3301 ggcgatacaa gagccctg
//
LOCUS       DAOTAC010000272         3308 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_22842_length_3308_cov_4.397787, whole genome shotgun sequence.
ACCESSION   DAOTAC010000272 DAOTAC010000000
VERSION     DAOTAC010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22842_length_3308_cov_4.397787"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="PLM74_10225"
     CDS             complement(<1..228)
                     /locus_tag="PLM74_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011877087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate enzyme"
                     /protein_id="HON43483.1"
                     /translation="MPSISGNLRCVLDNIAASAARAGRDPSEVRLVAVTKNVDVDRIC
                     SIVSAGIGHLGENRVQEAAEKVPEVPGSPTWH"
     gene            complement(281..1552)
                     /locus_tag="PLM74_10230"
     CDS             complement(281..1552)
                     /locus_tag="PLM74_10230"
                     /inference="COORDINATES: protein motif:HMM:NF024112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HON43484.1"
                     /translation="MWTTPRRKRPRLEERTPAERAQRGFAKAAAVVVVAVLSYSLFAA
                     VRPMISARLSRTAQAYSGWIEVSFPGDAVIVRDERVVVTPISGAIHFLVENGKRVAAG
                     AVVAEVRDRVAIEPPQSGSLAAQEEIDRFVQECAMREQREKERISALRVQVAIKEAEL
                     RAYTDRKDAKNANRLHAELSELHQREASAADSLARIQDETRAAAAAVAEAKRRSDTSS
                     TRALAVLRAFGASLISTHIDGLEGVYSPANPDLLDISPADYTPYPRVCSEGDSVAAGS
                     AVFREVQNLRTNLLVFAEVPEDAAPGAGSRVRVRFPRLATEAVPAAVIGSRRRPDISA
                     DTWAFHIALDRYATTLTDLRSESVTLVTERVSGLLIPRSALARKGDDDGVWLLRTNRF
                     IWRSVTVLGMNEEEAAVEGIREGDRVLCRPR"
     gene            complement(1699..2205)
                     /locus_tag="PLM74_10235"
     CDS             complement(1699..2205)
                     /locus_tag="PLM74_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Hint domain-containing protein"
                     /protein_id="HON43485.1"
                     /translation="MQSCLPENTELLIKDSAGEVSRVTLAYFDAMLDDEQGGPALSRV
                     RVLGRRGWVEFISISRRRLWEGEELLCVRTRSGQIATTCDHRIAVKRGLEDIVVPARD
                     LGTGDALMVFEQPALGKSARPIGEDPVFRPFGVLSVIRTVGYDGSVYQVETADHTLVA
                     NDFLMHTV"
     gene            complement(2382..2648)
                     /locus_tag="PLM74_10240"
     CDS             complement(2382..2648)
                     /locus_tag="PLM74_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004573553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HON43486.1"
                     /translation="MLRASELRVKEVVSVNDGKRLGYIFDLEIDMDTGKILSFVIPGG
                     GRILGFLGRGGELVLDWENIRKVGRDVVLVDDSELAGADGYTPG"
     gene            complement(2750..>3308)
                     /locus_tag="PLM74_10245"
     CDS             complement(2750..>3308)
                     /locus_tag="PLM74_10245"
                     /inference="COORDINATES: protein motif:HMM:NF021093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HON43487.1"
                     /translation="IAPGDDPQSQRLKFAVRDRVLEETGRLLQSADDANQAARILQAN
                     LPLIEAAAGEAVARSGTSHRVQAALGTFPFPGVLYGNISLPAGEYHALRVAIGPASGA
                     NWWCVLFPPLCPVDLERVRILDVAATTASDHLSGLHTSAGGRLFTLELAVRAKPLTLA
                     QLTWEMPSWACRILKWPQLQQARSR"
BASE COUNT          658 a         1075 c          948 g          627 t
ORIGIN      
        1 atgccaggta ggcgaccccg gcacctccgg gaccttctcg gcggcctctt gcacccggtt
       61 ttcgccaaga tggccgatgc cggccgatac aatgctgcat atcctgtcca catccacgtt
      121 cttggtgact gcgaccagtc gaacctcgga cgggtcacgc cccgcccgcg cagcagacgc
      181 cgcaatgttg tcgagcacac aacgaagatt accggagata gacggcaaga cttcccacct
      241 cgtacaaaac tagatattct ccgccatgct gcggattcct ctaccgaggc ctgcaaagca
      301 cccggtcgcc ctctcgtatt ccctcgaccg ccgcctcttc ctcgttcatg ccgagtacag
      361 tcacgctgcg ccagatgaac cggttagttc tgagcagcca cacaccgtcg tcgtcgccct
      421 ttcgcgcaag ggccgaacga gggatcaaca acccggatac tcgctccgtg acaagtgtga
      481 cactttccga gcgaaggtcg gtcaaggtcg tagcataacg atcgagggca atgtgaaaag
      541 cccaggtgtc ggcggaaata tccggccgtc ggcggctgcc tatcacagcg gcagggacgg
      601 cctctgtggc aagcctggga aagcgcacgc gcaccctgct ccctgccccc ggcgctgcgt
      661 cctcaggcac ttccgcgaag accagcaagt tggtgcggag gttctgcact tcccgaaaga
      721 ccgcggagcc ggcggcaacc gaatcgccct cggagcagac gcgcggataa ggtgtgtagt
      781 cggcgggcga aatgtcgagc aggtcgggat tggccgggct gtacacgccc tccagaccgt
      841 caatgtgggt gctgatgaga gatgcgccga acgcccgcaa cacggccagc gcgcgggtgg
      901 aagacgtgtc ggatcgtcgc ttggcctcag ccactgctgc tgcagcggct cgcgtctcat
      961 cctgaatccg ggcaagcgaa tcggccgcgg aagcctcgcg ttgatgcaat tctgacagct
     1021 cagcatggag cctgttagca ttcttggcgt ctttgcgatc ggtgtacgcc cgaagctcgg
     1081 cttccttgat cgcaacctga actcggagcg ccgaaatccg ttccttctca cgctgttcgc
     1141 gcatggcgca ctcctgcaca aaccggtcaa tctcctcctg agccgcgagt gaaccggact
     1201 gcggtggctc aatagctact ctgtcgcgca cttcggccac gactgctccg gctgccaccc
     1261 tcttgccgtt ctcaaccagg aagtgaatcg cgccgctgat cggagtgacc acaacgcgct
     1321 catcgcgaac gatcactgca tcgcctggaa aggaaacctc gatccaaccg gaataggcct
     1381 gggctgtcct ggacaacctc gctgaaatca tggggcgaac ggcggcgaag agagaatatg
     1441 aaagcactgc gaccacaacc acggccgcag ccttcgcaaa acctctctga gcacgctctg
     1501 ccggagtgcg ctcctcaagc ctggggcgtt ttcgccttgg agtagtccac atccctgtct
     1561 ctgcctctcg ctcttttcgg gcgcatggca cggataacca cacccgcctg accatcatta
     1621 tatcacggcg ggcttccagg cgctcctggc cgtattggcg ccaaggaatt gccccgctgg
     1681 catccgtatt ggcttcgact acaccgtgtg cataaggaag tcgttggcca ccagcgtgtg
     1741 gtcggcggtc tcgacctgat acacagatcc atcgtagcca acggttctga tgaccgagag
     1801 taccccgaag ggtctgaata ccggatcttc cccgatagga cgggcgctct tgcccagcgc
     1861 cggctgttcg aacaccatca gagcgtctcc tgtgccgaga tcccttgcag gtacgacgat
     1921 gtcctccaag ccacgcttga ccgcgatcct gtggtcgcag gtagtggcaa tctgccccga
     1981 acgggtgcga acgcacagca gttcttcgcc ctcccatagg cgcctgcgac tgatggatat
     2041 gaactccacc cagccgcggc ggccaagcac tctcaccctg ctgagagccg gaccaccctg
     2101 ttcgtcatcg agcatggcgt cgaaataggc cagcgtaacc cggctgactt cgcctgctga
     2161 gtccttgata agtagttctg tattctccgg caagcaactc tgcatcaaac atctcctcca
     2221 gcaggcgcag tatagcatca cgcggcccgg ctttcaaggt aaatcgatcc gcaggtaaat
     2281 cgatccgcaa acgatccgcc aggcgactga cggcccgagc ttgtcccccc tctgctgacc
     2341 tcatatcgac tgtcccaata ggcggttgat gcgcgccggg cctagcccgg cgtgtaccca
     2401 tcggcgccgg ccagttcgga gtcgtctacc aacactacgt ctctcccgac tttccttatg
     2461 ttctcccagt cgagcacgag ttctccgcca cgccccaaaa agcccaggat tctgccgcct
     2521 ccggggatca cgaagctaag gatcttgccc gtatccatgt cgatctccag gtcgaatatg
     2581 tagccaagcc gcttgccgtc gttgacgctg acgacttcct tgacacggag ttcggaagct
     2641 cggagaatca ttgaggcacc ccctgcaggc gatgatgacc cttcacattg tatgcaggag
     2701 gcaatggatg agtgcaaccg cggcgcagag tctgctggaa gagacgattc taccggctgc
     2761 gcgcctgctg gagctggggc cacttcagaa tgcggcaggc ccaggagggc atttcccagg
     2821 tgagttgagc aagggtcaga ggcttggcac gcactgcaag ctctagtgtg aagagcctgc
     2881 cgcctgcact ggtgtgcaag cccgagagat ggtcggaggc ggtggtcgcc gcgacgtcga
     2941 gaatacggac tctctccagg tccactgggc acaggggcgg gaagagcaca caccaccagt
     3001 tggcacccga agcgggcccg atggccactc tcagagcatg atactcccca gcgggcagcg
     3061 agatgtttcc gtacaggacg cccggaaaag ggaaagtgcc cagagcggcc tgtactcgat
     3121 gggaagtgcc gctccgggcc actgcttctc ctgccgccgc ttctatcagc ggaagatttg
     3181 cttgaaggat tcgcgctgcc tgattcgcat cgtccgccga ctgcaagagc ctgccggtct
     3241 cctcaagcac tcggtcgcgc acagcgaatt tcaggcgctg gctctgggga tcatccccag
     3301 gcgcgatg
//
LOCUS       DAOTAC010000273         3302 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_22884_length_3302_cov_3.590391, whole genome shotgun sequence.
ACCESSION   DAOTAC010000273 DAOTAC010000000
VERSION     DAOTAC010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3302
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22884_length_3302_cov_3.590391"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..1255)
                     /gene="spoIIIAE"
                     /locus_tag="PLM74_10250"
     CDS             complement(29..1255)
                     /gene="spoIIIAE"
                     /locus_tag="PLM74_10250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HON43488.1"
                     /translation="MNRVVAAVVIALIALSSGAVQAAGAAAATAATPAVKPSQGAVSS
                     SEALERAASEQLSQLDTGDIERFLRQVDDDVAQRLPAFDIKHIMSTNGTDMMDLPWIG
                     RELVGYLIHELQLNLKLLGELVVVAVVCTMLCLLGAGLGEDGPAAVAWAVCYMVMVVL
                     GVRSFSVSAEIAARAVDQMTSFMYAVIPALMGMLAASGGVVTAAVVSPFMLGATAAVG
                     TLVKTMVIPLLLMAVTLSLVGQISQRKQLSRLAKLLKWTGMTCLGLAATVFVAFTGMR
                     GGLASVSDGAAAKAVKFLSGNMVPVVGKVFGDALDLVATSSLLVRGALGAFGLATLAV
                     VCLFPVMKMTAIMLMFRIAAAVTQPLGDSRVSDCLDELGDALSSLCVCVATVGLMFFI
                     GVSIVLGLGGVVVAVR"
     gene            complement(1252..1641)
                     /gene="spoIIIAD"
                     /locus_tag="PLM74_10255"
     CDS             complement(1252..1641)
                     /gene="spoIIIAD"
                     /locus_tag="PLM74_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HON43489.1"
                     /translation="MLIAQVVGVAFLGCALLSLLRSARSDMAVAVSVSLGVVLFVLVV
                     SRLADFITAILGMASTAGVNRPYLDTVLRVVGVAYLAAFGAQVCRDAGEGALAVKVEL
                     AGKVLILGMAAPLMFALLDAVLQVLPQ"
     gene            complement(1662..1859)
                     /gene="spoIIIAC"
                     /locus_tag="PLM74_10260"
     CDS             complement(1662..1859)
                     /gene="spoIIIAC"
                     /locus_tag="PLM74_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007934040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HON43490.1"
                     /translation="MGLDLVLKIAGVGILVSVLCTILKQQGKEEYSLLVATAGLVVVF
                     LMLVQHIDELLSSVRSVFQLW"
     gene            complement(1873..2391)
                     /locus_tag="PLM74_10265"
     CDS             complement(1873..2391)
                     /locus_tag="PLM74_10265"
                     /inference="COORDINATES: protein motif:HMM:NF021090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43491.1"
                     /translation="MTVALKLAGGVLTVGAGGLLGLAIAESYRARERELAAWITALTA
                     LQTEISYGHTRLGEAMRRAGLASAGAPSRVLAHAAELLDGGESGGVGECLATALGARP
                     GDQRGEVDALVVLGRRLGTSDAADQARHIALAIQRLEGSRAKAEDAAARNCRVWSSIG
                     FLLGGMLTLALL"
     gene            complement(2388..>3302)
                     /locus_tag="PLM74_10270"
     CDS             complement(2388..>3302)
                     /locus_tag="PLM74_10270"
                     /inference="COORDINATES: protein motif:HMM:NF040318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43492.1"
                     /translation="DLYLCCAGQPVACTEDEWAAAVRLVTQCSVYALERELSAGFITL
                     PGGHRVGLVGRAVLDGDEVRTQTELSSMNYRVAREMPGVADRVMPYVLSADRTRVLNI
                     LILSGPGLGKTTLLRDIARQLSTGPECGSKWSWKCGLGPGPGWEPSQGASAPAGFRVG
                     IVDERSEIAACSQGVPQSELGPRADVIDGCPKASGIMMMIRSMSPDVLVTDEVGRHED
                     ADAIEEASRCGVAVIATAHALDPEDAARRPVLREVLARGAFDRAVVLGRTLGLGTCER
                     IVHLRSGQDLIARPFRLEGGVRVGGVRR"
BASE COUNT          721 a         1199 c          878 g          504 t
ORIGIN      
        1 ctcctatccg gctcggtcca cactcacctc acctgaccgc cacgaccact ccgccaagac
       61 ccaaaacgat cgagacgcca atgaagaaca tcaaccccac agtagccacg cacacacaca
      121 gcgacgaaag tgcatctccc agctcgtcca ggcaatccga cactcggctg tcgccaagag
      181 gctgtgtgac cgcggccgcg atccggaaca tcagcatgat ggcggtcatc ttcatcaccg
      241 ggaacagaca gaccacggcc agcgtcgcca agccaaacgc ccccagcgcc cccctgacca
      301 agagcgaaga cgttgccacc aggtccagcg cgtcgccgaa gaccttgccg accaccggca
      361 ccatgttgcc tgacagaaac ttgaccgcct tggcggcggc gccatctgaa acgctggcca
      421 atccgcctcg catgccggtg aatgccacga acaccgtggc cgccaggccc aaacaagtca
      481 tccccgtcca cttcagaagt ttggcgaggc gcgatagctg cttgcgctgg ctgatctggc
      541 ctaccaggct gagcgtgacc gccatcagga gcaggggtat caccatggtc ttcacaagcg
      601 tgcccacagc cgccgtagcc ccaagcatga atggcgacac gactgcggcg gttacgactc
      661 ccccagatgc agccagcatc cccatgagtg caggtatgac ggcgtacatg aaagacgtca
      721 tctggtccac agcgcgtgcg gcgatctcgg cggacacgga aaaagacctc acacccagca
      781 ccaccatgac catgtagcag accgcccacg ccacggcggc agggccgtcc tcccccagac
      841 ccgcccccag aaggcacagc atcgtgcaga ctacagccac caccactagc tcgccgagga
      901 gcttcagatt aagctggagc tcatggatga gataacctac aagctctctt ccgatccacg
      961 gcaggtccat catgtccgtt ccattggttg acataatatg ttttatgtca aatgccggta
     1021 gccgctgagc cacatcatca tcgacttgcc gcaggaaccg ctctatgtca ccggtgtcga
     1081 gctgcgacag ctgctcagaa gcggcacgct ccagggcctc cgaagacgaa accgcccctt
     1141 gcgagggctt caccgcggga gttgcagcag tagcagcggc agcgccggca gcctgaacgg
     1201 cccctgagga gagcgcaatc aaggcgatca ccaccgccgc aaccacgcgg ttcattgcgg
     1261 caacacctga agcaccgcat cgagcagggc gaacatcaag ggggcagcca tgcccagaat
     1321 tagcaccttg cccgcaagtt ccaccttgac ggccagcgcc ccttcgcccg catcccgaca
     1381 cacctgtgcg ccgaaagccg caagatacgc cactccaacc actcgaagga cggtatcgag
     1441 gtacggacga ttcactccgg cggtcgacgc catgcccagg atggctgtga tgaagtccgc
     1501 gaggcgcgac accaccagca cgaacagcac cactcccaaa gataccgaca ccgccacagc
     1561 catgtccgac ctggcgctcc tgagcaatga taggagcgca cacccgagaa aggccacccc
     1621 gacaacctgc gcgatgagca ttgcagttcc cctcatcgta atcaccacaa ttggaacaca
     1681 gaacgcacac tggagagcaa ttcgtcaatg tgctgcacaa gcatgagaaa gaccacaacg
     1741 agcccggcag tggccaccaa aagcgagtat tcctccttgc cctgctgctt aaggatagta
     1801 cacaggaccg acacgagtat ccctactccc gcgatcttga gcaccagatc cagccccacc
     1861 gccaagcccc ccttacagaa gcgccaaggt gagcatcccg cccagcaaga aacctatcga
     1921 cgaccagacc cggcagttgc gcgccgcggc gtcctcagcc ttcgctctgg agccttctag
     1981 cctctggatg gccagggcga tgtgcctcgc ctggtcggcc gcgtccgacg tgcccaggcg
     2041 acgccccaac accaccaggg catcgacctc accacgctga tcgcccggtc gggcgccgag
     2101 agccgtcgcc aggcactcac ccactcctcc ggattcaccg ccgtccagaa gctctgcggc
     2161 gtgcgcaagc acccgggacg gcgcgcctgc cgaggccagc ccggcgcggc gcatcgcctc
     2221 tcccagacgg gtatggccat aggatatttc agtctgaaga gcggtgagcg cagtaatcca
     2281 tgccgcaagc tcgcgttccc gcgcccgata tgattcggcg atcgccaagc ccagaagtcc
     2341 gccggcgccc acagtcagta cccctccagc cagcttcagc gccacagtca tcgccgtact
     2401 cccccgactc gcacgccgcc ctcgagcctg aacgggcggg cgatcagatc ctgacctgat
     2461 cgcaagtgca caatgcgctc acaggtgccc agaccaagcg ttcttccgag caccacagcc
     2521 cggtcgaagg cccctcgcgc cagcacctcg cgtagaaccg gccgacgcgc tgcatcctcc
     2581 gggtccagag cgtgagccgt ggctatcacc gcgaccccgc acctcgatgc ctcttcgatc
     2641 gcgtccgcgt cttcatgccg gcctacctcg tcagtaacca gaacatccgg cgacatcgat
     2701 cggatcatca tcatgatgcc ggaagccttc gggcatccgt cgatcacatc ggcgcgcggc
     2761 ccaagctcgc tttgtggcac tccctggctg cacgcggcta tctccgagcg ttcgtccacg
     2821 atccccaccc tgaatccggc aggcgcgctg gcgccttgac tcggctccca tcccggaccc
     2881 ggtcccaggc cacacttcca gctccacttg gaaccacact cagggcctgt tgagagctgc
     2941 cgggcgatat cccgaagcag agtggtcttg ccaagcccag ggcccgaaag gatcagaatg
     3001 ttgagaacac gggtcctatc tgccgatagc acgtatggca tcacccggtc ggccacgcca
     3061 ggcatctcgc gagctactct gtagttcatg ctcgatagct cggtctgggt gcgcacctcg
     3121 tcaccgtcga gcactgcccg accaaccagg ccgactcggt gaccgccggg gagagtgatg
     3181 aacccggccg atagctctcg ttccaaggcg tacaccgaac actgagtaac taggcgcact
     3241 gccgcggccc attcatcctc tgtgcaggct acaggttgtc ctgcgcagca taggtacaag
     3301 tc
//
LOCUS       DAOTAC010000274         3277 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_23095_length_3277_cov_4.454376, whole genome shotgun sequence.
ACCESSION   DAOTAC010000274 DAOTAC010000000
VERSION     DAOTAC010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23095_length_3277_cov_4.454376"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1138
                     /gene="argS"
                     /locus_tag="PLM74_10275"
     CDS             <1..1138
                     /gene="argS"
                     /locus_tag="PLM74_10275"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HON43493.1"
                     /translation="SVYARYMELRGEEYPFPEDGYRGEYVRDIAAGAMKDPSAARQLS
                     AMDADEARKFFREYALCHILDWQKRSLSQYQVHFDKWFSERELRATGAVEKMIGEMEE
                     AGHTYREDGALWFRSSEYGDDKDRVLIKSDGLYTYVAPDLAYHKDKLSRGFTKLIDIL
                     GPDHHGYEGRMQAGLKSFGYPDGTLEVLLLQLVTLMREGQPVKMSKRAGEFITMDDLL
                     EEVPKDAARYFFIMRNTSSHLDFDLDLARTQSMDNPVWYIQYAHARCCSIFRQAEAAG
                     ISDDAWAEADLALLAEPGELDLVRKMAAYPDEVLSAALERAPSRIAKYGLDLASLFHS
                     FYNECRVISDDAALTMARLALVDAAKTVIANALNVMGIAAPERM"
     gene            1141..2580
                     /locus_tag="PLM74_10280"
     CDS             1141..2580
                     /locus_tag="PLM74_10280"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HON43494.1"
                     /translation="MAERYSPAQALAVLGRSASIAYERMGMVVITSIAWYFAALAAAT
                     AAGVTVWAVPLAVVLVAPLSVAITYFGNLAVHREDAGLRELWRGYTKFWLRGVTLGAV
                     NLAIVFILWVDIRAIMAAKATWIRMLAGAWIYLGLFAGLLFMYAYPVMVEQDTTPWKS
                     IRRAVILILDNPAYTLTIGLLEVILLAVGVLPTVLLLSGSKAAGYLQIIGVAAYAGFN
                     SVFRNLAAVRLLQRYDAARESDRERLQKQLEVEKMAEGIPSTTGKPVEIRWLGHACFL
                     ITAGDGTRILTDPFDETVGYDLPTVPADIVTVSHAHFDHNNVGVVQGSPEVANSPGDR
                     SFGSVRIRGVRTCHDTKGGALRGHNIVFIIETDDIAICHAGDLGHVPSDETVKNIGRV
                     DVLLIPVGGTYTLDAKGAQEAVRKLDPKIVIPMHYKTPDLKMTELDTPERFLAGLSRV
                     ERAPGCSYTLQASSLPQDLTALVLGYRRK"
BASE COUNT          692 a          915 c         1018 g          652 t
ORIGIN      
        1 atcggtgtac gcccgctaca tggagctacg aggcgaggaa tacccgtttc cggaggacgg
       61 ctaccgcggc gaatacgtgc gggacattgc ggcgggggcc atgaaagacc caagcgcggc
      121 ccgccagcta tccgcgatgg acgccgacga ggccaggaag ttcttccgcg agtatgcgct
      181 ctgccacata ctggactggc agaagcgttc gctttcccag taccaggtgc atttcgacaa
      241 gtggttctcc gagcgcgaac tccgcgcaac tggggctgta gagaagatga tcggcgagat
      301 ggaggaggca ggccacacat atagggaaga cggcgcgttg tggttccgct cgtccgaata
      361 tggcgacgac aaagatagag tcttgatcaa aagcgatgga ctctacactt atgtcgcgcc
      421 cgacctggcc taccataaag acaaactgtc gcgtggattc accaagctaa tcgacatttt
      481 ggggccggat caccacgggt atgaaggccg gatgcaggcc ggcctgaaat ccttcgggta
      541 tcccgacggc actctcgaag tacttctgct gcagctggtt acgttgatgc gcgaaggtca
      601 gcctgtgaag atgtccaaac gggccgggga gttcataacc atggacgacc tgctggagga
      661 ggtacccaaa gacgccgctc ggtatttctt catcatgcgg aacacctcat cccacctaga
      721 cttcgatctc gacttggcac gcacccagag catggataac ccggtgtggt acatccagta
      781 cgcccatgcc cgctgctgca gcatcttcag gcaggcagag gcggctggaa tctccgacga
      841 cgcctgggcc gaggcggatc tcgcgctttt ggcggaaccc ggcgagctgg atttagtacg
      901 caagatggcg gcttaccccg atgaagtgct ttcagctgcg ctcgagcggg cgccgtctag
      961 gatagccaag tacggactgg acctggcaag cctcttccac agtttctata acgagtgtcg
     1021 cgtgattagc gatgacgccg cgctgaccat ggcccggctg gcgttggtcg atgcggcaaa
     1081 gaccgtgatc gccaacgcgc tgaatgtcat gggcatcgcc gctccggaga ggatgtagcc
     1141 atggcggaaa gatactcgcc ggcccaggct ctggccgtat tgggcaggag cgcatcgatc
     1201 gcctacgagc gaatgggtat ggtcgtgatc acgagtattg catggtattt cgcggctttg
     1261 gcggctgcaa cagccgccgg agtcacggtg tgggcggtgc cgctggctgt cgtgctcgtg
     1321 gcgccgctca gcgtggcgat cacttatttc ggcaacctgg ccgttcatcg cgaggacgcg
     1381 gggcttcgcg aactgtggcg cggctacacc aagttctggt tgcgaggggt tacgctgggc
     1441 gcggtcaact tagcgatcgt gttcatcctg tgggtcgata tccgggcgat catggcggcc
     1501 aaagctacgt ggatacggat gctggccgga gcctggatat acctaggact gttcgcggga
     1561 cttttgttta tgtatgcata tccggtgatg gtggagcagg acacaacccc gtggaaatcc
     1621 attaggcggg cagtcattct gatactggat aacccggcat acaccctgac aatcgggctt
     1681 ttggaggtaa tcctactggc ggtgggcgta cttccgaccg tgttactgct ctcaggcagc
     1741 aaagccgccg gatacctgca gattatcgga gttgcggcgt atgccgggtt caactcagtc
     1801 tttcgaaacc tggccgctgt gcggctgctg cagcgctacg atgctgcgag agagagcgac
     1861 cgagagagac tgcaaaaaca actggaggtg gagaagatgg cagagggtat tccttcgacc
     1921 acaggcaagc cagttgagat aagatggctc gggcacgcct gtttcctgat aactgccggc
     1981 gacggaacga ggatactcac ggatccattt gacgaaaccg tgggttacga tcttccgacc
     2041 gtgccggccg atatcgtgac cgtgagccac gcccacttcg accacaataa tgttggagtg
     2101 gttcagggtt ctccggaggt cgcgaactcg cccggcgaca gatccttcgg tagtgttcgt
     2161 attcgagggg tacggacatg ccacgacaca aaaggcggtg cccttcgcgg gcacaacatc
     2221 gtgttcatta tcgagactga cgacatcgcc atttgccacg cgggcgacct cggccacgtt
     2281 ccgtcagacg agacggtgaa gaacatcggt cgcgtcgacg tgctgctgat cccggtgggc
     2341 ggtacataca cgttggacgc caagggcgcc caggaggccg tgcgcaagct tgatccgaag
     2401 atcgtcatac ctatgcacta caagacccca gacctcaaaa tgactgagct ggataccccg
     2461 gagcgattcc tggcaggctt gtcccgagtt gagcgtgccc cagggtgcag ctacacacta
     2521 caggcttcga gcctgccgca ggatttgacc gcactcgtgc tgggctaccg aaggaagtga
     2581 gactggcggg atagggcagc gaccgcaccg cccgcaccgc ccgcaccgcc cagccgatgc
     2641 ccaccatttg ctcctacgcc tcgtagatgg ggttatcgcg gcgccgtacc tactaacggt
     2701 atccgaggag ctctgcccta tgggccaggg ttcccgggtg accccgacta tttcggggga
     2761 tgaatgccga taggcgcaag tcgtgggtgg taatggccca gtgctgggcc ttcagtgaaa
     2821 tcgcgtttag ctgggcgtta gtaaacctgt aaggaatgcg cccgatcttt tgcggtactc
     2881 gttcagaatg tgcccgggtt tagcgggctg gaataatagc caggctcaaa tggtaattgt
     2941 tcggtggccg agacccgtct gaggggcctc tactggggga cttaggaggc gcacctacga
     3001 taccatattg tgttattcgt ttctacctgc ggcttcaccg gccgtcagat tccataataa
     3061 ggaggggccg tcgccggcgc gaattcagac aattcgcacc gacaaaacca ggcaggatct
     3121 gaacaggttg actccggaac ctgttcaaag ctgactacac gttacccata tccatggatc
     3181 ggcccggata caaaggccga agcgcggata cccacatatt gcggctgaac gggaaaacgc
     3241 tgatgccgta ccatcggtac ccaaaccgcg ggagccg
//
LOCUS       DAOTAC010000275         3267 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_23200_length_3267_cov_4.007472, whole genome shotgun sequence.
ACCESSION   DAOTAC010000275 DAOTAC010000000
VERSION     DAOTAC010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23200_length_3267_cov_4.007472"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1109
                     /locus_tag="PLM74_10285"
     CDS             <1..1109
                     /locus_tag="PLM74_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HON43495.1"
                     /translation="FVQPKLKGVKAVYEPIADGYDRWLVNSLSAGTGPDLFYVDIFWS
                     RGLFSSGAVESLDSYIAKSKMLKTTDITPSLLNAFTYNGKIYGIPKDFNTLALFYNRD
                     MFDVAKIPYPSENDDWNTLTDKLRKVQAGLPGVYGIALAPEYARMGPFAYAAGFSVFD
                     SKGKSNVTGAQFREGFEFYTGLAKKGVGIMPADIGQGWGGGAFAAEKVAACLEGAWII
                     GFLRDQSPNLNYGATLLPKNPRTGQRGNLIFTVSWSMNAQSKHKQEAFQVLELLTSPE
                     AQQWVLERDLALPSRQALIDNPYFKRDDKEAAANYIVFRGASDGNVFPFEFAAYGDDW
                     MTPINQVLSAVMSGEQTVDVALKEAQKRLDELMK"
     gene            1218..2330
                     /locus_tag="PLM74_10290"
     CDS             1218..2330
                     /locus_tag="PLM74_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HON43496.1"
                     /translation="MRPRGVSHTWEKAAPYILLAPFIFHIAAFFGYAFARTFYFSFTE
                     YNLFASPKWVGLANYVDLFRNVQFGRALKNSLQFAVIVTLAQTFLALILAVVMNSRVR
                     GIKFFRAAYYMPSVTSSVVITLIFMWLFQRGGVINYLTTKFIEYRAVIGVFLACLAVI
                     QVLLVLRDRAGGWPASWFEPAFLLMSAAFSFVLVLAFQAARVIGPNPNVEAVRTVWLN
                     TRQTVPGWAGALAIPRPLAAIMMLNTWTTAPTMMLLFLAGLQDIPRELYEAAAVDGAG
                     PAAAFRHITLPGLRSVTFLVLSMGLISTLQMFDQVAIIGEQAPREAVITLAYFVYSNA
                     FPGSAVPRIGAASAAAIVLAVFTMVLVSLQKRITRS"
     gene            2352..>3267
                     /locus_tag="PLM74_10295"
     CDS             2352..>3267
                     /locus_tag="PLM74_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HON43497.1"
                     /translation="MRLDEGIAEQDRHAQAENRRKLAARRRWTKAAAVYVLLLGFSLL
                     FVGPMAFAMLSSLKDNPVEWPPRLGSPQLSPANWVAAARLGVAGGGSWLFGEFARGAV
                     VPFSIEYEVDEGREPETPIVRVPRRVPGAGPAAIVAEPFAADDAVVSEVREVNRVTTD
                     GVVRVTYEFDITHAGSDMVYGKLPLDIEVPMYQRFVRATLEPNRIERLGRVQSWNNVT
                     SGVIPYVFYNYHRVFRENYSRATGKNLFAAWIRNSFLLAGIKVITTLVVASMAGYALA
                     RLKLFGRNALFNLVLFSVMIPGQVTFVSN"
BASE COUNT          621 a          933 c         1022 g          691 t
ORIGIN      
        1 aattcgtgca gcccaagttg aagggcgtca aggcagtgta cgaaccgatt gctgatggtt
       61 acgacagatg gcttgtgaac tcgctgtcag ccggaacagg gcccgatctg ttctatgtgg
      121 atattttctg gtctcgcgga ctgttctcgt ccggtgcagt tgagtctctc gactcgtaca
      181 tagctaagtc caagatgctc aagacaaccg acatcacgcc ctcgctgctc aacgcgttta
      241 cttacaacgg caagatctac ggtattccca aggacttcaa tacgctcgca ctattctaca
      301 accgcgacat gttcgacgtc gcgaagatac cctaccctag tgaaaacgat gactggaaca
      361 ccttgactga caagctccgc aaagtgcagg caggtctacc cggggtgtac ggaatcgccc
      421 tggcgcctga atacgctcga atgggcccgt ttgcgtacgc agccggtttc tcggtgttcg
      481 actccaaggg caaatcgaac gtaacaggcg cccagttccg tgagggtttc gagttctaca
      541 ccggtcttgc caagaagggc gtaggcataa tgcccgccga catcggccag ggctggggcg
      601 gcggggcgtt cgcagccgag aaagtcgccg cgtgcctcga aggcgcctgg ataatcgggt
      661 tcctgcgcga tcagtcgccc aacctgaatt acggcgccac gctgctgccg aagaaccctc
      721 gcaccgggca gcgcggaaac ctcatattca ccgtttcgtg gtcgatgaac gcgcagtcga
      781 agcataagca agaggccttc caagtgctgg agctactcac gagccctgag gcccagcagt
      841 gggtgctcga gcgggatctg gccctgccgt cgaggcaggc cttgatcgac aacccgtatt
      901 tcaagcgtga tgacaaagag gcagccgcta actacatcgt gttccgcggc gcatccgacg
      961 gcaacgtgtt cccatttgag ttcgccgcgt acggcgatga ctggatgacc cctatcaacc
     1021 aggtgctgtc ggcggtcatg agcggcgagc agaccgtcga tgttgcactg aaggaagcgc
     1081 agaagcgtct ggatgagctc atgaagtaag gcggtgagcg gtggcgccgg ctctggggat
     1141 gggtcctgct atgcatcggg acccatccgc ggccgcccgc tcgaaacttg agcttgaggg
     1201 aaaggaggcg caggccagtg aggccgcgtg gggtctccca cacctgggag aaggctgcgc
     1261 catacatcct gctggcgcct ttcatcttcc acatagccgc attcttcggg tacgcgtttg
     1321 cgcggacctt ctacttcagc ttcaccgagt acaacttgtt cgcaagcccc aagtgggtgg
     1381 gccttgcaaa ctacgtggac ttgttcagga acgtccagtt cggccgggca ctgaagaact
     1441 cactccagtt tgccgtcatc gtgacgctgg cccagacgtt cctggcgctg atcctggccg
     1501 tggtgatgaa ctcccgggtg cgcgggatca agttcttcag ggcggcctac tacatgccca
     1561 gcgtcacgtc gagcgtggtg ataaccctaa tcttcatgtg gttgttccag aggggcggag
     1621 tcatcaacta cctgaccacc aagttcattg agtatcgtgc ggtgattggg gtgttcttag
     1681 cgtgtcttgc agtcattcag gtcctcctgg tcctgcgcga tcgtgccgga ggatggcctg
     1741 catcatggtt cgagccggca tttctcctca tgtcggccgc cttctctttt gtgctggtgt
     1801 tggcgtttca ggcggcgcga gtgatcggcc ccaatccgaa cgtggaggct gttcggacag
     1861 tgtggctgaa cacgaggcag actgtgcccg ggtgggcagg agcgttggcc attccgcggc
     1921 cgttggccgc gattatgatg ctcaatactt ggactacggc gcctacaatg atgctgttgt
     1981 tcctcgcggg cctgcaggac attcccaggg agctatatga ggccgccgcg gtggacggcg
     2041 ctggcccggc agcggcgttt cgccacataa ccttgcctgg cctgcgcagc gtcaccttcc
     2101 tcgtgctgag catgggcttg atcagcacat tgcagatgtt cgatcaagta gccatcattg
     2161 gagagcaggc gccgagggaa gccgtcatca cattggccta ttttgtctac agcaacgcat
     2221 ttccgggatc ggcagtaccg aggataggcg ccgcgtctgc agcagccatt gtgctggccg
     2281 tctttactat ggtgctggtg tctttgcaga agcggatcac aagatcatga gccatgggcg
     2341 aggtgcgagc gatgagactg gatgagggca tagctgaaca ggatcggcac gcccaggccg
     2401 agaaccgaag gaagcttgcg gcccggcgcc gatggacgaa ggcggcggcc gtatacgtac
     2461 tgctcctcgg cttttcgctg ctctttgtgg gcccaatggc gtttgccatg ctgtcgagct
     2521 tgaaagacaa ccccgttgag tggcctccgc ggctgggatc gccgcagcta tcgccggcta
     2581 actgggtcgc ggccgcgaga cttggcgtgg cgggaggagg gagttggctg ttcggggaat
     2641 tcgcccgggg cgccgtcgtt ccgttctcca tagagtacga ggtcgacgaa ggcagggaac
     2701 cggagacacc catcgtgcgt gtgccgcggc gggttcccgg cgccgggcct gccgccattg
     2761 tcgcggagcc gttcgccgcg gacgacgctg tcgtcagtga agttcgtgag gtcaatcgcg
     2821 taacgactga cggcgtggtg cgggtcacgt acgagttcga catcacacat gcggggagcg
     2881 acatggtcta cggcaaactc cctcttgaca tcgaagtgcc catgtaccag aggttcgtcc
     2941 gggccaccct tgagccgaac aggatcgagc ggcttgggag ggtgcagagc tggaacaacg
     3001 ttacgtccgg cgtgatcccg tatgtgttct acaactacca cagggtattc cgcgagaact
     3061 acagccgcgc cacaggcaag aacctcttcg ccgcgtggat ccgcaactcg ttcctgcttg
     3121 cggggatcaa agtcattacg actctggtag tagcttcgat ggccggttat gccctggcaa
     3181 ggctgaagct cttcggccgg aacgccctgt tcaatctggt tctcttttcg gtgatgatcc
     3241 cggggcaggt gacgttcgtc tccaact
//
LOCUS       DAOTAC010000276         3244 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_23430_length_3244_cov_3.997178, whole genome shotgun sequence.
ACCESSION   DAOTAC010000276 DAOTAC010000000
VERSION     DAOTAC010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23430_length_3244_cov_3.997178"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1282
                     /locus_tag="PLM74_10300"
     CDS             <1..1282
                     /locus_tag="PLM74_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43498.1"
                     /translation="VMWARSTDGGDPAVGLLGYELEIRSQAGGQTRDMGILIHTSGGL
                     LSPSGDDTEQYISEALPLSSGTPYTAWIRAIDLLGNESDWFGAPFIFDPGRPGDPGKP
                     ALEPWPNAGNTLSFGWTHATDARAGVPESGVDLYEFQIKGIDNPLWDVLDAAITVDIV
                     EDEDVDLVDPDAPLTGNCVFTLPTRLVDGNYVARVRAKDVAGNYSNWVESDPFSVTLD
                     REGPTVVIMTPADTTTTNLSTFTWTWVVEDDEDSPGIRGYWAKLNDEAWSWTTEPVFT
                     SSRLHRGANVLRVKGVDNLGNEGPEAEAPAVMLVDAVIFDVHPEPGAHRINEVSTIAF
                     SVTGLHDGTVEVLLGNGPLEDAWRLLSVFRTPELAQFYILLDADVMQPGMLTVTIRIG
                     DATRTCDYRVLSERTGFGFGRLRPGDRSAPMEKR"
     gene            1577..>3244
                     /locus_tag="PLM74_10305"
     CDS             1577..>3244
                     /locus_tag="PLM74_10305"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HON43499.1"
                     /translation="MNTRNKSHLLLLSLLVLVMSFAAPALATDSTADSALAALAQQKA
                     STLVNDLGVTSVQYAVISNGEIILSGHAGVYSRTDDRPITADTMYGIGSTSKVFTTAA
                     IMILVDSGKVALDAPVTEYIPEFTMADPRYRQITVRMLLNHSSGLMGSTFRNAFLCND
                     PDQAARDMLLADLASQRLKAAPGEFSVYCNDGFTLAELVVERVSGESLTDFIRHNITV
                     PLGMANTATPQDAFDRSRLARTYVDALYGYAVVDTIGVIGTGGIYSTAEDLCRFARIF
                     MNDPGADGADGILSVPSRSAMAASEYAKGIWPVQRCSFVGYGLGWDAVSSYPFDYYGM
                     KAPAKGGDTQLYHSTLLVLPTANVAAAIVSSGGSSALNLMVASELLQQLLESRGILTA
                     ARPEVPNGKPVAAVMPPTLLGHSGLYGGEEGLLTVDISADGRLTVTSLGEGASRAATY
                     TYTAEGVFVSEDGRAQMSFVTEANGHTYSKRVAFINIPSLGHTVITDYFAQRLQPLAL
                     EEDVLNAWRARDGVSYYVVSDKFTSQVYIAGPSLRVSVSKEEPGYVGT"
BASE COUNT          686 a          951 c          991 g          616 t
ORIGIN      
        1 tgtcatgtgg gcccgatcga ccgacggcgg cgaccccgca gtcgggctgc tcggttatga
       61 gctggaaata cggtctcagg cggggggcca gacccgcgat atgggcattc tgatccacac
      121 ctcaggcggg ctgcttagtc cttcaggcga tgacaccgag cagtacatct cggaggcgct
      181 gccgttgtca tccggaactc catacaccgc gtggatccgc gccatcgacc tgttgggcaa
      241 tgaatcagat tggttcggcg cccccttcat attcgatccc gggaggcccg gggaccccgg
      301 taagcctgcc ctggagccat ggccgaacgc tggaaacacc ctgtcgtttg ggtggacgca
      361 tgcgacagat gccagagcag gggttcccga gtcaggcgtc gatctctacg agttccagat
      421 caaagggatt gacaaccctc tctgggacgt cttggatgcc gcgatcactg tggacattgt
      481 tgaggatgaa gatgtcgacc tggttgatcc cgacgctccg ctgaccggca actgcgtatt
      541 cacactgccc acccggctgg ttgacgggaa ctacgtggcc cgggtcaggg cgaaggacgt
      601 ggcgggcaac tattccaact gggtggagtc cgatcccttc tcggtcacac tcgatcgtga
      661 gggcccgacc gtagtcataa tgactccagc tgacacgact acgactaacc tatccacatt
      721 cacctggaca tgggtagtcg aagacgacga agacagcccg gggatcaggg gctactgggc
      781 gaagctgaac gatgaagcct ggtcctggac gacagagcct gtctttacgt cgtcgcgcct
      841 gcaccgtggg gcaaacgtgc ttcgcgtgaa gggcgtggac aacctgggca atgaaggccc
      901 tgaagccgaa gcgcccgcgg tcatgcttgt tgacgcggtg atcttcgacg ttcaccccga
      961 gccgggcgcg caccggatca atgaggtgtc gacgatagcc ttcagcgtga cagggctcca
     1021 tgatggcacg gtcgaggtgc tcctcggcaa cggacctctc gaagatgcat ggcgactctt
     1081 gtccgtcttc agaacaccgg aattggccca gttctacatc ctgctggacg cagacgtaat
     1141 gcagcccgga atgctcaccg tgaccatcag aatcggagat gccactagga cctgcgacta
     1201 cagggtgttg agtgagcgga caggctttgg ctttggccgc cttcgaccag gcgaccggag
     1261 tgccccgatg gagaagcgat agcggggagg tcctgatcga tctggtgaag gcagccgccg
     1321 gatctgttga atccgcagaa tgcttcggat tggggagact tcccatcagg gaaggctggt
     1381 agaagaccaa aggaccagac taagggaggg cgcgcaccgc gagtgcgcgc ccttcggttt
     1441 ggggacgttg agggcccgtc cagatgacgg ccacaaagtg aagacaatat gatacactga
     1501 taggcaagta ataacactta atgcctacag gtgggaacaa atcggaatat cgcagtagga
     1561 ggtgcgtaag aacagaatga acactcgaaa caagtcacat ctgctactat tatcgcttct
     1621 ggtcctagtc atgtcgttcg cggcccctgc actggcgacg gactccaccg ctgattctgc
     1681 tctggcagcg ctggcacaac agaaggcctc gacgctcgtg aacgatctag gggtgacaag
     1741 cgtccagtac gcagtgatat ccaacgggga gatcatcctt tcaggccatg ccggcgtgta
     1801 cagcagaacc gacgataggc cgatcactgc cgacaccatg tacggaatcg ggtctaccag
     1861 caaggtgttc accacagcag cgatcatgat cctagtcgat agcggcaagg tcgcgctcga
     1921 cgctcccgta accgagtaca tacctgagtt caccatggcc gacccgcgct accggcagat
     1981 caccgtgcgc atgctgctta accattcatc gggcctgatg ggctcaacct tccgcaatgc
     2041 gttcctttgt aacgaccccg accaagccgc ccgcgacatg ctcctcgccg atctggcgag
     2101 ccagcggctg aaggcggcgc ccggggagtt ctctgtgtac tgcaatgacg gcttcaccct
     2161 ggctgagctt gtggttgagc gggtgagcgg agagagcctc accgatttca tcaggcacaa
     2221 catcaccgtc cctctgggga tggccaacac ggccacaccg caggatgcgt tcgaccgcag
     2281 ccgactggcg cgaacatatg tcgatgcact atatggttac gccgtcgttg ataccatagg
     2341 cgtgatagga acaggcggca tctactccac agctgaagat ctatgtagat tcgcccgcat
     2401 tttcatgaac gacccgggcg ccgatggagc cgatggcata ctgtcggtac cgtcccgctc
     2461 ggccatggcc gcctccgaat atgccaaagg catctggccg gtgcagcgct gcagtttcgt
     2521 agggtatggc ctcgggtggg atgcggtaag ctcatatccc ttcgactact acggcatgaa
     2581 ggctcctgcc aaaggcggcg acacccagct ctaccattcg accctgctgg tgctcccgac
     2641 ggccaatgtc gcagccgcga tagtgtcgtc cggcgggtcg agcgccctca acctgatggt
     2701 ggcgagcgaa cttctgcagc aactgctgga atccaggggc attctcactg cagccaggcc
     2761 cgaggtgccg aacggcaaac ctgtcgctgc cgtcatgccg ccgacgctcc ttggccattc
     2821 ggggctatac ggcggcgaag aaggccttct aacggtggat atctccgccg acggcaggct
     2881 gaccgtaaca tccctgggcg aaggcgcctc gcgggcagcg acttatacgt atactgcaga
     2941 gggcgtgttc gtgagcgagg atggccgcgc acaaatgagc ttcgtgacgg aggcgaatgg
     3001 acatacgtac agcaaacgcg tggcgttcat taacatcccc agcctggggc acacagtcat
     3061 caccgactat ttcgcgcaga ggctacagcc tcttgcgctt gaagaggacg tgctcaacgc
     3121 ctggcgcgcg agggacggag tgtcatacta cgtcgtgagc gacaagttca cctcgcaggt
     3181 gtacatcgct ggaccgtcgc tcagggtgag tgtctcgaag gaagagcccg gttatgttgg
     3241 cacg
//
LOCUS       DAOTAC010000277         3239 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_23487_length_3239_cov_4.047425, whole genome shotgun sequence.
ACCESSION   DAOTAC010000277 DAOTAC010000000
VERSION     DAOTAC010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23487_length_3239_cov_4.047425"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="PLM74_10310"
     CDS             <1..704
                     /locus_tag="PLM74_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HON43500.1"
                     /translation="CASRDMSPGAVVRRCREVGLDIVAITDHNAAGNVARAMAEAVSG
                     PTTGPVIIPGMEVCTSEELHVLTLLPDLARATQWEAVVRRALTPGENVPKIFGEQWVQ
                     DACTGALCSDTALLAAPTRLSLADVVSQVTDLGGACIPSHVDRAAFSILGQLGFVPEG
                     LDLAGLEISRAINREEALLRMPQIARYSLISSSDAHSLDEIGLGCSLLWIEEPTVAEI
                     ILALRGARGRRVEIE"
     gene            701..1243
                     /locus_tag="PLM74_10315"
     CDS             701..1243
                     /locus_tag="PLM74_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002575933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HON43501.1"
                     /translation="MRELSMHILDIAQNSIAAGAKMVRIDVVEDHAADIMTITVADDG
                     SGMDAETAQRIRDPFVTSRTTREVGLGIPLFAAAAERCGGRLEIISAPGVGTTVRAIF
                     GLSHIDRAPLGDMAGTLMALSVCNPDVDFVYNRVQGDESFHFDTRDVRAELGSVPLNH
                     PEVAAFIRDYVEQGEGRLGR"
     gene            1240..1731
                     /locus_tag="PLM74_10320"
     CDS             1240..1731
                     /locus_tag="PLM74_10320"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HON43502.1"
                     /translation="MKQTGVVQSAHGPYVDVLVKRPDACHGCGACKLASDEPRVVTAR
                     NDVGALAGDIVEIELSGATLMRAVGLAYGVPLVMFMLGLLLGEPIARRIGLAINPSLA
                     SATMAFIFLAAGYYIIHHYDRSLGAGAFMSVATAIVDSEQVECAPEGCVAAERFSGSE
                     ENR"
     gene            1728..2381
                     /locus_tag="PLM74_10325"
     CDS             1728..2381
                     /locus_tag="PLM74_10325"
                     /inference="COORDINATES: protein motif:HMM:NF003995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HON43503.1"
                     /translation="MRKAKIPDVVVRRLPLYLRAVDDFDRREHVIVSSKELGDFTGLT
                     PAQVRKDLTFFGEFGKQGIGYDVKFLRNQLRDILRLSTDVHIGLAGLGNLGQALVHYH
                     VDARSRPRTRFSVSPDRLRIVAAFDVNPAKVGRAYAGVPIFHIDQLGEKVQEYGLKIM
                     VNAVPAEYAQDVVDRIVACGIKSILNFAPVTLNVPENVRVHSADLTLELQSLAYYAE"
     gene            2412..>3239
                     /locus_tag="PLM74_10330"
     CDS             2412..>3239
                     /locus_tag="PLM74_10330"
                     /inference="COORDINATES: protein motif:HMM:NF015237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HON43504.1"
                     /translation="MSSRKLQAIILVVLVLVVAGAARASMQQEKSEYCGSCHTMKPYY
                     ESWKKSSHADVECAECHSDRGVGGWIQLRRDLGRMTRVEKSGAQPDLDIEITDEFCLR
                     CHTKAQNIKEGESLIIPHGLHDAAGLDCTPCHGGAVHGEAGEGPARMTHDTCIACHED
                     IISDEESCMKCHKSVDLAETANMIIPHDTHSFMSCGSCHGPQAPPEARQAFDMGHDQC
                     VQCHEDIISDPDSCTTCHKVPDVAETADLKIPHGMHVSIPCGQCHGPQLPTEAKAAYA
                     "
BASE COUNT          681 a          897 c         1035 g          626 t
ORIGIN      
        1 catgcgcgtc ccgggacatg agcccgggcg ccgttgtcag aagatgccgc gaggttggac
       61 tggacatagt agccataacc gaccacaacg ccgcaggcaa tgtcgcccga gcgatggctg
      121 aggcggtctc agggccgacg acagggccag tgataatccc tggtatggaa gtgtgcacct
      181 ctgaggaact tcatgtgctc acccttctgc cggatctggc ccgtgcgacc cagtgggagg
      241 ctgtcgtgag gcgcgcgcta acccccggag aaaacgtgcc caagatcttt ggcgaacaat
      301 gggtgcagga tgcgtgcaca ggagcgttat gcagcgacac agcgctgctt gctgcgccca
      361 cacgtttatc gcttgcagat gtagtgagcc aagttactga cctcggggga gcgtgcattc
      421 cgtcccatgt agacagggcg gctttcagta tcctgggtca gttaggattt gtcccagaag
      481 gactggacct tgcggggctg gaaatctccc gggcaatcaa ccgcgaggag gcgcttctgc
      541 gtatgccgca gattgcccgc tacagcctga tatcgtcgtc cgatgcacat tcgttggacg
      601 agataggcct gggctgttcg cttctttgga tcgaagaacc taccgtggcc gaaatcatcc
      661 tggccctacg cggagcccgg ggcagacggg tggagatcga atgagagagc tgtctatgca
      721 catactcgat atcgcccaga actccatcgc agcaggcgcg aaaatggtca gaatagacgt
      781 agtggaggac catgccgccg acatcatgac catcaccgtg gccgacgatg gttccgggat
      841 ggacgccgag accgcgcagc gcattcgaga ccctttcgtg acatcgcgaa ccacgcgcga
      901 ggtcgggttg ggaattcccc tgtttgcggc ggcggctgaa aggtgcggcg gacggctcga
      961 gatcatcagc gcgcccggcg tgggcacgac cgtaagagca atattcggcc tgtctcacat
     1021 cgaccgtgcg cccctcggcg acatggccgg cacgttgatg gcactttcag tatgtaaccc
     1081 ggacgtggat ttcgtgtata atcgtgttca gggagatgaa tccttccact tcgatacgag
     1141 ggatgtccgg gcagagctag gcagcgtgcc gcttaaccat ccggaagtag ccgcgttcat
     1201 tcgtgactat gtagagcagg gcgaggggag gctggggcga tgaagcaaac aggcgtcgtg
     1261 cagagcgccc acgggcctta tgtggatgtt ctggtcaagc gcccggatgc atgccacggg
     1321 tgtggagcgt gcaagctggc gagcgacgag cccagggtcg taaccgccag gaacgatgtg
     1381 ggtgctctag caggcgatat tgtagagatc gagctttcgg gcgccacact catgcgcgcc
     1441 gtgggcctag cgtacggcgt gccgctggtc atgttcatgc tcgggcttct gctgggcgag
     1501 cctatcgcga ggcgaatcgg gcttgccatc aacccgtcgc tggcctctgc aaccatggca
     1561 ttcatcttcc tagccgcagg ctattatatc attcaccatt acgaccgttc gctgggagct
     1621 ggcgctttca tgtcggtggc gacggcaatc gtcgattctg aacaggtgga gtgtgccccg
     1681 gaaggatgcg ttgcagccga gcgattcagc gggtcagagg agaaccgatg agaaaggcga
     1741 agattccgga tgtagtagtg aggaggcttc cgctctacct tcgggcggtc gatgatttcg
     1801 atcggcggga gcacgttatc gtatcctcca aggagttggg cgactttacg ggccttactc
     1861 cagctcaagt caggaaggac ttgacattct tcggcgagtt tgggaagcag ggaatcggct
     1921 atgatgtgaa gttcctccgc aatcagctgc gggatatact gaggctcagc accgacgtgc
     1981 acatcggcct ggccggattg ggcaatctcg ggcaggcgct ggtccattac catgtagacg
     2041 cgcgttccag gccgaggacg aggttctcgg tttcgccgga caggctccgt atcgtggctg
     2101 cgttcgacgt caatccggcc aaggtaggta gggcgtacgc cggcgttcca atcttccaca
     2161 tcgatcagct gggcgagaag gtgcaggagt acggtctgaa gatcatggtg aacgccgtgc
     2221 ctgcagagta tgcccaggac gtggtcgatc gcatcgttgc gtgcggtatc aagtctatcc
     2281 tgaacttcgc gcctgtgact ctgaacgtgc cggaaaacgt gagagtgcac tcggcagatc
     2341 ttacgttgga gctacagagc ctggcgtatt acgcagagtg atcgggtgta cgtgcaaagg
     2401 gggtccacaa gttgtcgtcg cgcaagttac aggcgatcat cttggtagtg ctggtactgg
     2461 tggtcgcagg cgctgcaaga gcgtctatgc agcaagagaa gtcggaatac tgcggttcct
     2521 gtcacacaat gaaaccatac tacgagtcat ggaagaaatc gagccacgct gacgtggagt
     2581 gcgcagaatg ccacagtgat cggggcgtgg gcggttggat ccagctccgg cgcgacctgg
     2641 gccggatgac tcgcgtggag aagtcgggcg cccagccgga tctcgacata gagatcaccg
     2701 atgagttctg cctcagatgc cacaccaagg cgcagaatat caaggaaggc gaatcgctga
     2761 tcataccgca cgggttgcac gacgcagccg ggcttgactg cacgccatgc cacggcggcg
     2821 cagttcacgg cgaagccggc gaaggccctg cccggatgac tcacgacacc tgcatcgcct
     2881 gccatgagga cataatctca gacgaagagt cgtgcatgaa gtgtcataag agcgttgacc
     2941 tggccgaaac cgccaacatg atcatacccc acgacacaca cagcttcatg tcgtgcggat
     3001 catgtcatgg accgcaggcc ccgccggaag cccggcaggc cttcgacatg ggccacgacc
     3061 agtgtgtaca gtgccacgaa gacatcatca gcgatcccga ttcctgcacc acatgccata
     3121 aggtgccgga tgtagcggag acggcagacc tcaagatccc ccatggcatg cacgtgtcaa
     3181 ttccttgcgg ccagtgccac gggccccagt tgccgacgga ggccaaggca gcctatgca
//
LOCUS       DAOTAC010000278         3233 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_23531_length_3233_cov_4.334487, whole genome shotgun sequence.
ACCESSION   DAOTAC010000278 DAOTAC010000000
VERSION     DAOTAC010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23531_length_3233_cov_4.334487"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..152)
                     /locus_tag="PLM74_10335"
     CDS             complement(<1..152)
                     /locus_tag="PLM74_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HON43505.1"
                     /translation="MRHAVIGTAGHVDHGKTTLIRALTGVNTDRLRQEQERGLTIELG
                     FAPLTLP"
     gene            complement(149..1759)
                     /gene="selA"
                     /locus_tag="PLM74_10340"
     CDS             complement(149..1759)
                     /gene="selA"
                     /locus_tag="PLM74_10340"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HON43506.1"
                     /translation="MTRSSFLRSIPSISDVLEHPVAIELAARVPRWAVRNAARKLISE
                     QRARLERLARDQVAGGIGDQVAAEGVAATEVIPGAEAAPNRASILDAVLQALPAVAER
                     CATPGPRPVINATGIVLHTNLGRAPLPDYVAWAVADCARGYSDLEFDLETGERGSRQD
                     HVAIQIARTCGAEAAYVVNNNAAAVLLCLDTIARGREVLVSRGELVEIGGSFRVPDIM
                     ERSGARLVEVGTTNRTRISDYRRAVNPEVGLLLKVHTSNFRIVGFTEEVDITELVSLG
                     RETGLPVMYDIGSGLMADMSCYGMGSEPTVLGAVSAGADLVTFSGDKLFGGPQAGIIV
                     GRADLIERISRNPLARALRIDKLTLCALAAVAAAWEDRDAAMESVSVASMLAVEPGAL
                     ARRAARLAELIRLELDHARVDPAGVDISVVDDPSEAGGGSLPTVSIPGASVCIKPRCV
                     SCEEIQRRLRMGDPPVVARVADDSIRLHLRTIFDRDFGRLATATASAIACGHASTAER
                     IARPSATGAGGTGEETGNPSGTGGNVVS"
     gene            complement(1857..2915)
                     /gene="selD"
                     /locus_tag="PLM74_10345"
     CDS             complement(1857..2915)
                     /gene="selD"
                     /locus_tag="PLM74_10345"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(2862..2864),aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HON43507.1"
                     /translation="MDERDIVRLTSLSTKAGUAAKIGPGDLTLALAGLVHRTDPNLIA
                     GFEGSEDAGVYKLTDDIAIVQTCDFFTPILDDPYEFGQVAAGNSLSDVYAMGGKPLIA
                     MNIVCYPCSLGMEPLREILRGGADKVWESGALLVGGHSVDDPEPKYGLSVTGIVHPDQ
                     VVLNRGAQPGDVIVLTKPLGTGTVSTALMAGMADSQAQRRLYKTMSALNAEASRAMVE
                     SGAHACTDITGFGFLGHLMEMAEASGVSFEVYAGQIPVIEGALEYAEMGLIPGGLRRN
                     QDFLAGKVEANDVEQSVLEVMFDPQTSGGLLIAVAPDRVDDLVGRLRRAGASVLTAAV
                     VGRTVSAGSEGPRVRVVR"
BASE COUNT          624 a         1087 c          924 g          598 t
ORIGIN      
        1 ggcagcgtga ggggagcgaa cccaagctca atcgtgaggc cccgttcctg ctcctggcga
       61 agccggtcag tgttaacgcc tgtaagcgcg cggatcaagg tggtcttgcc gtggtcaaca
      121 tggcccgccg ttccgatcac cgcatgtctc atgaaaccac atttcctccc gtacctgacg
      181 gattccctgt ctcttcccca gttccgcctg ctccggtcgc agacggccta gcgattcgct
      241 cggcagtcga tgcgtgtcca cacgcgatgg ccgatgccgt tgccgtggcc agccggccaa
      301 agtcgcggtc gaatatggtc ctgaggtgaa ggcggatgga gtcgtcagcc actcgggcca
      361 cgactggagg atcgcccatt cggaggcgcc gctgtatttc ctcgcacgag acgcagcgcg
      421 gcttgatgca cacactcgcg ccgggtatcg acactgtggg gagcgaaccc cctccggctt
      481 cggatggatc gtctacgact gagatgtcga cacctgcggg gtcgacgcgg gcgtggtcca
      541 gctccagccg gatcagctcg gcgagtcgcg ccgcccgccg ggcgagagcg cccggttcca
      601 cagcgagcat gctggctacg gacactgatt ccattgccgc gtcgcgatct tcccatgcag
      661 cagccaccgc cgccagagcg cacagggtaa gcttgtcgat acgcagggcc cgtgccagag
      721 ggtttcggga aatgcgctcg atcagatcgg cacggcccac gatgatgccg gcctgcggtc
      781 ctccgaacag cttatcgccg ctgaacgtga ccagatcggc gcctgccgac acagcgccca
      841 ggactgtagg ctcggagccc attccgtaac acgacatgtc cgccatcaag ccggacccaa
      901 tgtcgtacat caccggaaga ccggtctcgc ggcccaggga cacaagctca gtgatatcga
      961 cttcctctgt gaagcccact atgcggaagt tggaggtgtg caccttgaga agcagcccga
     1021 cctcggggtt cacggccctt cggtagtcag agatgcgagt tcggttggtc gttccgacct
     1081 caaccagacg agcgccgctc cgttccatga tgtcgggcac cctaaacgag ccgcctatct
     1141 ccaccagctc tccccggcta accaggacct cgcgcccgcg ggcgatcgtg tccaggcaga
     1201 gcaggaccgc agcggcgtta ttgttcacca cgtacgcagc ctcagcgccg cacgtgcggg
     1261 cgatctggat cgctacgtgg tcctgacgtg atcctcgttc gcctgtctcc aggtcgaact
     1321 caagatcaga gtatccgcgc gcgcaatcgg caactgccca agccacgtaa tccggcagcg
     1381 gggctcggcc taggttggtg tgaaggacta ttccggtggc gttgatcacc ggacgcggcc
     1441 ccggggttgc gcacctttca gccacggcag gaagcgcttg tagaaccgcg tcgaggatcg
     1501 aagccctgtt cggggcggcc tccgctcctg ggattacttc agttgccgcg actccctcag
     1561 ccgccacctg atcaccgata ccgccggcga cctgatcgcg cgcgaggcgt tccagacggg
     1621 cgcgctgctc ggaaatgagc ttccgtgccg cgttccgcac cgcccaccgc ggcacgcgtg
     1681 ccgccagttc aatcgccacc ggatgctcaa gaacatccga aatcgatgga atgctccgaa
     1741 ggaaagaaga ccgagtcacg cctctccacc tacctgcctg atacgtccat ggatgacacc
     1801 atgctctgcc taaccctggg ccacatgggc cttataggcc tcctggaaac ctcctgctat
     1861 ctcactaccc tcacacgcgg gccttcgctg cctgctgata ctgtccggcc caccaccgcc
     1921 gcggtgagca cggaggcgcc cgctctgcgc agcctcccca caagatcgtc cactctatcg
     1981 ggcgcaaccg cgatcaacag ccccccggac gtctgtggat cgaacatcac ctcgagcaca
     2041 ctctgctcga catcgttcgc ctctaccttg cctgccagga agtcctggtt acgccgcaac
     2101 cccccaggta tcaagcccat ctcggcatac tcaagtgcgc cttctatcac cgggatctgg
     2161 cctgcatata cctcaaacga aaccccggac gcctccgcca tctccatcag atggcccagg
     2221 aagccaaagc cggtgatgtc ggtgcatgca tgagcgccag actccaccat ggcccgggac
     2281 gcctcggcgt tgagagccga catggtcttg tagaggcggc gctgggcctg actgtcggcc
     2341 atgccagcca tgagagccgt agacactgtg cctgtgccga gcggtttggt caacactatc
     2401 acatcaccgg gctgagcgcc ccggttgagc acaacttgat cggggtgaac aatccccgta
     2461 accgaaaggc catacttagg ctcggggtcg tccactgagt gcccgcccac aaggagcgct
     2521 cccgattccc acaccttatc agccccgccg cgaagaattt cgcgaagagg ttccatgcca
     2581 agcgaacacg ggtagcacac tatgttcatt gcgatcagcg gctttccgcc catcgcatat
     2641 acgtcggaca gcgaatttcc cgccgctacc tgcccgaatt cgtaggggtc gtccagaatt
     2701 ggcgtaaaga agtcacacgt ctggacgata gctatgtcgt cagtcaactt gtacacgccc
     2761 gcgtcctccg aaccctcgaa acccgcgatc aggttagggt cggtgcggtg cacaagtccg
     2821 gccagagcca acgttaggtc gcccggacct atcttcgccg ctcagccagc tttggtcgac
     2881 agtgatgtaa gcctgacgat atcccgttcg tccatgaccc gtccccccca ctttcctcgc
     2941 ctgtatcata ctctagttca gccgccccag aaagtccacc gttcgtctgt tatggttacc
     3001 ctgctccatc ctcgatcagc agtcgacgtc acgtcaacga gcctgactga taggggtacg
     3061 tgcagcgatt gtggcgccga taggcagtgt gccgaatacg aagtgggagt gactgactac
     3121 cgggccggcg acctgcttgg gctcggaaac gatagtcaca ggcgaaaaag gctttgcgca
     3181 ggtcttcagc gaaaggggcg tctagcaggg gcttagcgag cctacaacaa atg
//
LOCUS       DAOTAC010000279         3223 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_23618_length_3223_cov_4.699179, whole genome shotgun sequence.
ACCESSION   DAOTAC010000279 DAOTAC010000000
VERSION     DAOTAC010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23618_length_3223_cov_4.699179"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..999
                     /locus_tag="PLM74_10350"
     CDS             <1..999
                     /locus_tag="PLM74_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004067418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HON43508.1"
                     /translation="GPTTTMIKSRAPVVAICAVRTGVGKSQTTRYVSQALIDAGLKVV
                     AIRHPMPYGDLAKQAVQRFATYDDLDVHECTIEEREEYEPHIDRGVIVYAGVDYGKIL
                     EAAEKEADVILWDGGNNDFSFYVPDLMITLADPHRPGHEVSYHPGETNIRMADIVIIN
                     KVDTAKPEDIEVVRKNVAAVNPDAIIIDAASPVSVDNPGMIRGKRVLVVEDGPTLTHG
                     GMGYGAGIVAAHKFGAASLVDPRPFAVGSIVDTLNRFHHREPLLPAMGYSKRQVQELE
                     KTIKNSDAEVIVIGTPIDLRRVLKIDKPAVRVGYELEQTAGPDLKQILLKKFARKS"
     gene            1175..2599
                     /locus_tag="PLM74_10355"
     CDS             1175..2599
                     /locus_tag="PLM74_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43509.1"
                     /translation="MATKDRIRAAARRPLSVHVLALAVAAAMSGTIASAVGASTAAPQ
                     TPAPALVGAGTLSLELKAKSEADPELRVSATYRMGQVSSSLQYRPGGALELKAGANLT
                     EDWRQFRHSGSLAFSGSIDPADRKASWNQISMSIGSRAHKNAILWQPESKMELSVRSY
                     PDNPVRDYAEAKLTAKAGKTLPGEARPTVKGELSLGHKEMPEQPKWTADFWSSMISLQ
                     WRPVSDVQATASLSAKGRAYPEASHKSYTTYGGKAGATWAVSKQHSLQFAASAASTVR
                     AGDPSKDRRTADASAKWTLKTPAASVASLMAGSLTLTARVTAGATLRPNLPEDNVAWR
                     AGASSGLVWELSKTLKLSFAAETAWSGTVSDDDPADDDDQGDSDNGNDENDYYAQQLP
                     DMLPQSAHPDSGPMRATASSVGISSATGKATHRLSVSVTAGPYRGVTFSGKAITARTD
                     ELGQGIWSDGVWAPSLELKASYKF"
BASE COUNT          715 a          963 c          986 g          559 t
ORIGIN      
        1 gggcccacca cgaccatgat caagtccagg gcgccggtag ttgcgatctg cgcggttcgc
       61 acaggcgtcg gcaagagcca gaccacccgc tacgtgagcc aggcactgat cgatgcaggc
      121 ctgaaggtcg tggcgatcag gcatccaatg ccatacggcg acctcgccaa acaggcagta
      181 cagcgcttcg ctacatatga cgacctcgat gtgcatgagt gcacgatcga ggagcgcgag
      241 gagtacgaac cccacatcga ccgcggcgtg atcgtgtacg ccggcgtcga ctacggcaag
      301 atcctcgagg ccgccgagaa ggaagcggat gtgatcttgt gggacggcgg aaacaacgat
      361 ttctccttct acgtgcctga cctcatgatc accctggccg atccgcatag acctggccac
      421 gaggtgtcat accatcccgg cgagaccaat atacgcatgg cggacatcgt gatcatcaac
      481 aaggtcgata ccgcgaagcc cgaggatatt gaggttgtgc gcaagaacgt ggcggccgtt
      541 aatccggatg cgatcatcat cgacgcggca tcgcctgtca gcgtggataa ccccggcatg
      601 atccgtggca agcgggtgct ggtagtggag gacggcccga cgctcaccca tggcgggatg
      661 ggctacggcg cgggaatagt tgcagcccat aagttcggcg cggcatccct cgtcgacccg
      721 aggcccttcg ctgtcggcag catcgtggat accctcaacc gtttccatca ccgcgaacct
      781 ctgctcccgg cgatgggata cagcaagagg caagttcagg agctggagaa gacgataaag
      841 aactcggacg ccgaagttat cgtcatcggc acaccgatag acctgagaag ggttctgaag
      901 atcgacaagc cagcagtgcg cgtcggctat gagctggagc agactgcagg cccggatctg
      961 aagcagattc tactgaagaa gttcgcccgc aagtcttgac accttaggct cgatctccgc
     1021 gggcctgtgg aggtcgagcc tggatagcca gcggcgttct cccccgaacg ggcgatttcc
     1081 accgtgcgca tttacagcca tcacctaaca tggtatgatc tgtatatgac ggtgggaatg
     1141 accgtggtca atatctcgct ggggaggatg aacgatggcg accaaagacc gcattcgtgc
     1201 cgccgcccgg cgtccgttat ccgtgcacgt actggcactt gccgtggcgg cggccatgtc
     1261 aggtacaatt gcgagcgccg taggcgcatc caccgctgca ccccaaaccc ccgcacctgc
     1321 actggtggga gctggcacgc tcagcctgga gctgaaggcc aagtcagagg cagatccgga
     1381 gctgagggtg agcgcgactt accgaatggg ccaagtgtcc tcatccctcc aatacaggcc
     1441 gggaggggcg ctagagctca aggccggcgc caacctcact gaagactggc ggcagttccg
     1501 ccactcggga tcgctcgcgt tttccggcag catcgaccct gccgatcgca aggcatcctg
     1561 gaaccagata tccatgtcca taggcagccg ggcccacaag aacgctatct tatggcagcc
     1621 tgagtcgaaa atggaattat ccgttcggag ctatccggac aaccctgttc gggactatgc
     1681 cgaggccaag ttgacggcga aggcggggaa gacgcttccc ggcgaggcta gaccgacggt
     1741 caaaggcgag ctgagcctgg ggcacaagga aatgcccgag cagcccaagt ggacggccga
     1801 cttctggagt tcaatgataa gcctccagtg gcgcccggtg agcgacgtgc aggccacggc
     1861 aagcctgtcg gccaaaggac gggcataccc tgaagcgtct cataagtcgt acactacata
     1921 tggaggcaaa gcgggtgcaa cgtgggcggt atccaaacaa cactcgcttc agttcgctgc
     1981 ttctgctgca tccacagtcc gcgcaggcga cccatccaag gaccggagga ctgccgatgc
     2041 ttcagcgaaa tggacactta agacgcctgc cgcttcagtc gcatcgctta tggcgggttc
     2101 attgactctc actgctcgcg ttacagccgg ggcaaccctt cggccgaatc tgccggaaga
     2161 taacgtggca tggagggcgg gggcaagctc cgggctggtc tgggaactgt cgaagactct
     2221 caagctatcg ttcgcggccg agacagcctg gtcgggtact gtgagcgatg acgacccggc
     2281 agatgacgac gaccaaggcg atagcgacaa cggcaacgac gaaaacgact actacgccca
     2341 gcagttgccc gacatgctgc cacagagcgc tcacccggac agcgggccga tgcgggcaac
     2401 cgcgagttct gtgggcataa gcagcgccac cggcaaggcc actcaccgcc tgagcgtttc
     2461 agtaaccgcc ggcccctacc gcggcgtaac cttcagcggc aaggcgatta ctgcgcgcac
     2521 agacgaactg gggcagggca tctggagcga tggagtatgg gctccatccc tggagctcaa
     2581 ggcatcctac aagttttagg gaccaatacg gctcgtccgt gtcttcaagg ccagattgtg
     2641 tcgaccgttc gggcacactc tggcctcctc gcctgactgc tacgaccagc agccacaatg
     2701 tcacccatga acaaggggga cgtcatcttg accgagaagg tacagaagag agccttcgtt
     2761 atggaccagc ttatcgccac acagatgaca aacgcgctgg ccgacctcac aggcagcgta
     2821 atccatcgga tacgccggcg ctcgtagtcg ctgtggatcg ctccggctgg gctcgggccg
     2881 gcgtagtgct tcatgacggg cgtctgcgcg acctgccttc cggctggtac ctaagcgtcg
     2941 cttcactgga atggccgttc agctggtctt gacagcatgg ccgtcagaca aaacggctgg
     3001 agctggcata tcgtcggtca aaaagtgacc gtatctctgg gcagataccc ccgggggtat
     3061 atcactgagc cacagctgtg gacctggcga gattgcacac ctgaatgcgg agtatctgcg
     3121 accccgaacg caacaatcgt tacacacccg cccggagatt cgcccggagg atagcgtaat
     3181 cttagtgtag gctgctgggt agggaaaagc ggagtacagc aag
//
LOCUS       DAOTAC010000280         3216 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_23684_length_3216_cov_4.538437, whole genome shotgun sequence.
ACCESSION   DAOTAC010000280 DAOTAC010000000
VERSION     DAOTAC010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23684_length_3216_cov_4.538437"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="PLM74_10360"
     CDS             <1..849
                     /locus_tag="PLM74_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HON43510.1"
                     /translation="AGHTDRAISREEIMEGVRYIRDTPEVRDVLISGGDPLTLADDAL
                     EFIIRELRAIPHVEIIRIGTRTPVVMPQRITEDLCAMLRKYHPIWLNTHFNHPKEITQ
                     ESRRACEMLVDAGIPVGNQSVLLKGVNDCPHIMKKLVHELVKMRVRPYYIYQCDLSMG
                     IEHFRTSVGKGIEIIEKLRGYTSGFAVPTYVIDAPGGGGKIPVGPQYLISQTDTQVIL
                     RNYEGVITAYAEPRDKISECHECGICKEKPPLATDGIAAVMDGTVSNLEPVGLARRKR
                     ARKHSS"
     gene            797..1906
                     /locus_tag="PLM74_10365"
     CDS             797..1906
                     /locus_tag="PLM74_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43511.1"
                     /translation="MNQSDLRGGREHASTLLDLVLAGGNCAGVVGLAKNCGKTTTLNA
                     LIQVAAERNIAVGITSGGRDGELYDAITGLPKPPIRVPRGALIALADFTIGRTSCRLE
                     VVQRLGVHTTAGEIVIARALEPGNVEVIGCNRANDLIHAVSKLRKCGAELALVDGAAG
                     RTFLASPDIVQTFVLATGAALDSDVQGIADATRMAVHLFTLPAPPPRIARRAEQAIES
                     GVSAIMRADGRMDSLPWPTLLGREPLVAAMLSSGDLGLVCARAVGDGLLAALTSRFRE
                     HSARPEVFRVVAADPSRIAAGDRGVKAFEAAGGELAVLRKAGVAAITVNPSSPSGTMI
                     NPSDLVMEVSKHIEELPVFDMVAELSASGGVMLET"
     gene            1896..>3216
                     /locus_tag="PLM74_10370"
     CDS             1896..>3216
                     /locus_tag="PLM74_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018360056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 5,6-aminomutase subunit alpha"
                     /protein_id="HON43512.1"
                     /translation="MKLDLDISKIDRCRECASSIADQVMEHVRSVTTTSCERAVLRLL
                     GVDGVDRAGVPLPNVVVDIAHKAGLLPDGIATYMARAVASTGLSPQQVAEKVALKQLD
                     LASFGSAPVGGERAVVSGAVSASLGRIRENVAKRREYLSRLDSGTEPLIYVIVATGNI
                     YEDVEQAQSAAKAGADIIAVIRSTAQSLLDYVPYGATTEGYGGTFATQENFRIMRKGL
                     DSVSAEIGRYIRLVNYCSGLCMPEIAAMGALERLDMMLNDSMYGILFRDINMKRTFAD
                     QYFSRMINAYAGIIINTGEDNYLTTADAYEEAHTVLASQFINEALAHRSGLPDSQIGL
                     GHAFEMNPEMKDGFLYEVAQAQMSRDIFPNAPLKYMPPTKYMTGDIFRGYLLNGMFNL
                     AGVMTGQSIQLLGMLTEAIHTPHVHDRYLAIKNAKYVFGSARHLGEDI"
BASE COUNT          698 a          913 c         1004 g          601 t
ORIGIN      
        1 gcgggtcata ccgacagagc gatatcgcga gaagagatca tggagggcgt tcgctacata
       61 cgggatacgc ccgaggtgcg agacgtgctc atatccggcg gcgacccgct aactttggca
      121 gacgacgcat tggagttcat aatacgcgag cttagggcca tacctcatgt ggaaatcatc
      181 agaatcggaa cgaggactcc agtggtcatg ccgcagcgca tcaccgagga cctctgcgcc
      241 atgctcagga agtaccaccc gatctggctg aacacacact tcaaccatcc aaaagagatt
      301 acccaggagt cgcgccgggc atgcgaaatg ctggtcgatg cgggcatacc tgtgggaaat
      361 cagagcgtcc tgcttaaggg cgtgaacgac tgtccacaca tcatgaagaa gctagtgcac
      421 gagctggtca agatgagggt gcggccgtac tacatctatc aatgcgacct gtccatgggg
      481 atcgagcact tcaggacgtc tgtgggcaag ggcattgaga tcatcgagaa gctgaggggc
      541 tacacatcgg ggtttgccgt gcctacgtat gtgatcgacg cacccggagg cggcggcaag
      601 ataccggtgg gtccgcagta cctgatatcg caaaccgaca cccaggtcat actgcgcaac
      661 tatgaaggag taatcaccgc gtatgccgag ccgcgcgaca agatcagcga gtgccacgag
      721 tgcggcatct gcaaggagaa accgcctctc gctacagacg gcatagcggc cgtaatggac
      781 gggacggtgt cgaatcttga accagtcgga cttgcgagga ggaagagagc acgcaagcac
      841 tcttcttgac ctcgtgctgg ccggcggcaa ctgcgcgggg gtcgtggggc ttgccaagaa
      901 ctgtggaaag accactaccc tcaacgcatt gatccaggtt gccgctgagc gcaatatcgc
      961 agtgggcatc acatcgggcg gcagagacgg tgagctgtat gatgccatca cgggtcttcc
     1021 caagccaccc attagagtcc cccgcggggc tttgatcgcg ttggccgact tcactatcgg
     1081 gcgtacatca tgccgtcttg aggtcgtgca gaggttgggc gttcatacga cggccggcga
     1141 gattgtaatc gcccgcgcgc ttgagccggg gaatgtggaa gtgatcggat gcaaccgagc
     1201 aaacgatctg attcatgccg tcagcaagct gcgcaaatgc ggggcagagc tcgcactggt
     1261 ggacggtgca gccggccgaa ctttcctcgc gtcgcccgat attgtgcaga cattcgtgtt
     1321 ggccaccggg gcagcactcg acagcgacgt gcagggaatc gccgatgcca cgcgcatggc
     1381 ggtgcatctg ttcacactgc cggcgccgcc tccgcggatt gcgcgccgcg cggagcaggc
     1441 catcgaatca ggcgtatcag ccataatgcg agctgacggc cgcatggact cactgccatg
     1501 gcccactctt ctggggcgcg aaccccttgt ggctgctatg ctcagtagcg gggacctggg
     1561 gctcgtatgc gcgcgtgcag ttggcgatgg gctacttgct gcgctcacat cgcgctttcg
     1621 cgaacactct gcacggcctg aggtgttccg agtggtggct gccgaccctt cgagaatcgc
     1681 cgccggcgac cgcggtgtga aggccttcga ggcggccggc ggggaactgg cggtgctcag
     1741 gaaggcaggt gttgcggcta tcaccgtcaa cccttcatcc ccgtcaggga cgatgattaa
     1801 cccgtctgat ctggtgatgg aagtatcgaa gcacatcgaa gaactgccgg tcttcgatat
     1861 ggtggcggag ctttcggcat ctggaggtgt catgcttgaa acttgatctc gatatcagca
     1921 agatagacag gtgcagggaa tgtgcgagca gcattgcgga tcaggtcatg gagcacgtac
     1981 gcagtgtgac gacgacctcg tgtgagcgag ccgtgctccg tctactgggc gtggatggag
     2041 tagaccgtgc gggggtacct ctgcccaatg tggtagtaga catagcgcac aaagccgggc
     2101 tcctcccaga cggcatcgca acatacatgg ctcgtgccgt cgcgtccacc gggttgtcgc
     2161 cgcagcaagt ggccgagaaa gtagccctga aacagctgga ccttgcatct ttcgggagtg
     2221 cgccagtcgg cggcgagcgg gcggtcgtgt caggagcggt gtccgcctcg ctcggtcgca
     2281 tacgcgaaaa cgtcgccaag cggcgcgagt acctgtcgag gctcgattcc ggcacagagc
     2341 cgctcatcta cgtcatcgtg gccacaggta acatatatga ggatgtggaa caggctcagt
     2401 cggcagcgaa ggcaggcgca gacatcatag cggtgatcag gagcaccgcc cagagccttc
     2461 ttgactatgt gccctacggc gcgacgaccg aaggctacgg cggcaccttt gccacgcagg
     2521 agaactttcg cataatgcgg aaggggctgg acagcgtgag cgcggagatc ggccgttaca
     2581 tccggcttgt gaactactgc tccggcttgt gcatgcccga gatagcggca atgggcgcac
     2641 tggagcggct ggacatgatg ctcaacgact ccatgtacgg cattctcttc cgcgacatca
     2701 acatgaagcg tactttcgcg gaccagtact tctcgcgcat gatcaacgcc tacgccggta
     2761 ttatcatcaa tacgggcgaa gacaactacc taaccaccgc ggatgcctat gaagaagccc
     2821 acacagttct ggcatcgcag ttcatcaatg aggccttagc gcacaggtcg gggctgcccg
     2881 attcacagat cggcctcggg cacgcgttcg agatgaaccc ggagatgaag gacggatttc
     2941 tgtatgaggt ggcgcaggcg cagatgtcgc gcgacatctt tcccaatgcg cccctaaagt
     3001 acatgccgcc taccaagtac atgactggcg acatattccg cgggtacctg ctaaacggca
     3061 tgttcaacct ggcgggtgtg atgaccggcc agtccattca gctcctgggc atgctcaccg
     3121 aagcgattca cacgccacac gtacacgacc gatacttggc catcaagaac gccaagtacg
     3181 tgttcggtag cgctaggcat ctgggcgaag acatca
//
LOCUS       DAOTAC010000281         3190 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_23921_length_3190_cov_3.889952, whole genome shotgun sequence.
ACCESSION   DAOTAC010000281 DAOTAC010000000
VERSION     DAOTAC010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23921_length_3190_cov_3.889952"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..1343)
                     /locus_tag="PLM74_10375"
     CDS             complement(102..1343)
                     /locus_tag="PLM74_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008395528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HON43513.1"
                     /translation="MFTYTQHMTDDCMGAYKTFAKDEYTRRLGRLQKNMARHGCDVLL
                     LTSPENITYVTGYRTWYFSSKFRPVICVVPQAGEPALVLRILEKSTVQLTSWAPHVYC
                     WGSASRNLGPLQAADPATATALAVRELAGSPATVGIEAGEGARFNAPLTLLDELRAAM
                     GGISFIDGTPAICDTRMIKTEWEASRLRKACKATEDSIEAAFARVKPDETTESDLARW
                     IAAGMAERGVDRISYLTIISGDAKYSTFNAYATGRIIRRGDIVLADISGHIEGYASDL
                     TRTAIVGRPSAEQARMAQVANDSVMRCVEAMRPGVPISRISRVAEDTIREAGYGQYVV
                     HSSGHGIGLDVVEYPMIHHSQETILEPGMTFAVEQGVYPYRPEDGAEGIYWCFRTEDV
                     VRVTETGAEFLSGPGPALIEL"
     gene            complement(1363..2988)
                     /locus_tag="PLM74_10380"
     CDS             complement(1363..2988)
                     /locus_tag="PLM74_10380"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HON43514.1"
                     /translation="MAVFLGIDIGTSVIKAVAVRSDGEILGVGRYTYTPSRYSDGRVE
                     HDPEAYWTGACLATRDALAAASAATAGLEVAAIGLTGMAPSTVFVDADGEPLGLSPIW
                     QDMRARDEVAEFGAAVCAAVGSDSVRGITGMVPSPSFSGPKLKWMARNAPDLYARTRV
                     ILDAKDFVAMRMCGSVATDETSAASSLLFDLGRRRWSAPLIDAMGVDGAKLPLVKKSL
                     AVVGVTNSRFASRVAEANGSTGENCPVDPRDLEGIPVVIGAGDSLATAFLFAASEDDA
                     LGRPTLEISGTSTCLMRQTPKPAVDPQGRLMCFPAADGHSYCVEAAIHTTGACLDWIS
                     RTLGMELGDGSQAAGTRPGPMRVDWSKVERVPAGADGLVFLPYIGPGERSPIWDDSAR
                     GAFIGITSMHSPEHMVRAVMEGVAFALRQNLDAVEELSGPWGIIESVGGPSANRVWQQ
                     IKADVLGRPVATLAAREATGCAAAAMAAAGVWRWDAVDAIRRANTRRTAVYRPISANT
                     AMYDALFGVFARSYQRLKEEFATIAELLRGHGA"
     gene            complement(2992..>3190)
                     /locus_tag="PLM74_10385"
     CDS             complement(2992..>3190)
                     /locus_tag="PLM74_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HON43515.1"
                     /translation="TFDIVYVLTSGGPSNRTELLSFHIYRHGFLYARFSYATAVSVLL
                     LIGILVFTFILARMTMNEEKA"
BASE COUNT          598 a         1062 c          924 g          606 t
ORIGIN      
        1 ctgtactccg cgtttccctc cccagcagcc tacactaaga ttacgctatc tcgcgccttg
       61 tgttgcgcgc ctgttgcctc tacagctcga tcaaagctcg atcaaagctc gatcaaggca
      121 gggccggggc ccgacaggaa ctcggcgccg gtctcagtga cccgaaccac gtcttcggtc
      181 ctgaaacacc agtatatccc ctccgcaccg tcttccggcc ggtatgggta aaccccctgt
      241 tccacagcaa aagtcatgcc cggttcgaga atggtctcct gagagtggtg tatcatcggg
      301 tactccacta catccagccc gatgccgtgc ccgctggagt ggaccacgta ttgaccgtat
      361 ccggcctccc taatggtatc ctccgcaacc cggctgatcc gcgatatggg caccccaggt
      421 ctcatcgctt cgacacagcg catcacggag tcattggcta cctgggccat cctggcctgt
      481 tctgcgctgg gccgaccgac tatcgccgtg cgcgtcaggt cgctggcgta tccttctatg
      541 tgtccgctta tgtcggccaa cacgatatca ccccggcgga tgatcctgcc tgtggcgtag
      601 gcgttgaagg tagaatactt ggcgtcgccc gagatgatag tgaggtacga gatccggtcc
      661 acaccgcgct cggccatgcc ggcggcgatc catctggcga ggtcggattc agtagtctcg
      721 tccggcttta cgcgggcaaa cgcggcctcg atggagtcct ccgtcgcctt acaagccttg
      781 cgcagacggg acgcctccca ctcggtcttg atcatccggg tatcacagat agcgggggtg
      841 ccgtctatga aactgatccc gcccattgct gcccgaagct cgtccagaag agtcagagga
      901 gcattgaacc tggccccttc gccggcctct atccccactg tggccgggct gcccgcgagt
      961 tcacgcacgg ccagagctgt ggctgtcgcg ggatcagccg cctgaagcgg gcccaggttg
     1021 cggctggcgc ttccccagca gtagacgtgg ggcgcccacg atgtcagctg cacggtgctc
     1081 ttctccagta ttctgagcac tagggccggc tcgcctgcct ggggaacgac gcatatcacc
     1141 ggacggaatt tgctcgagaa gtaccacgtc cggtatccgg tcacgtacgt gatgttctca
     1201 ggactggtca gcagaagcac gtcgcacccg tgcctcgcca tgttcttctg caaacggccg
     1261 agccgtcgtg tatattcatc cttggcgaat gtcttgtaag cgcccataca gtcgtccgtc
     1321 atgtgctgcg tatatgtgaa catccgtgaa acctccgtct agctacgcgc catgaccacg
     1381 aagcagctct gcaatcgtgg cgaactcttc cttcaggcgc tggtatgatc gggcaaagac
     1441 tccgaatagt gcatcgtaca ttgctgtgtt ggctgaaatc gggcgataga cggctgttct
     1501 gcgcgtattc gcgcggcgaa ttgcgtctac tgcatcccaa cgccagacgc cggccgccgc
     1561 catagctgcc gcggcgcagc ccgtcgcctc gcgagcggcg agcgtggcca ccgggcgccc
     1621 cagcacatcc gcctttatct gttgccatac acggttggcg ctgggcccgc ccaccgattc
     1681 aatgatgccc cacgggccgc tcagttcttc cacggcgtcg aggttctgcc gcagagcgaa
     1741 ggccacgccc tccataaccg ccctgaccat gtgctcaggc gagtgcattg acgtgatgcc
     1801 aatgaacgcg ccgcgagcag aatcatccca gatcgggctg cgctcgccgg ggcctatata
     1861 aggtagaaag accagaccgt cggcgcctgc cgggacgcgc tccactttgg accagtccac
     1921 gcgcatgggt ccaggtctcg tccccgccgc ttgagaccca tctcccaact ccatgccgag
     1981 agtgcgggag atccaatcca ggcaggcccc tgtggtgtgg atcgccgcct cgacacagta
     2041 gctgtggccg tcggcggccg gaaaacacat gagcctaccc tgagggtcga ccgcaggctt
     2101 gggcgtctgc ctcatcaggc aggtggacgt gcccgatatc tccagcgtcg gccggcccag
     2161 cgcgtcatct tcggaggcag cgaacagaaa cgccgtggct agcgaatcgc ctgcgccgat
     2221 caccaccgga atgccctcca gatcccgcgg atccaccggg caattctcgc cggtagagcc
     2281 gttcgcttct gccactcggc tcgcgaagcg actgttcgtc actcccacca cggcgaggga
     2341 cttcttaaca agcggaagct tggccccgtc cacgcccatg gcgtcgataa gcggcgccga
     2401 ccatcgccgt ctcccaagat cgaaaagcag gctactggca gcggatgttt cgtcagtggc
     2461 caccgatcca cacatccgca tggccacaaa atccttagcg tccagaatca cgcgcgtgcg
     2521 tgcgtataag tcgggggcgt ttcgtgccat ccatttcagc ttgggcccgg aaaacgaagg
     2581 actggggacc atccccgtga tcccgcgcac ggagtcggac ccgaccgcgg cgcagaccgc
     2641 ggcgccgaac tcggcaacct cgtcgcgcgc gcgcatgtcc tgccagatgg ggcttaggcc
     2701 aagcggttct ccgtcggcgt ccacaaacac agtgctgggc gccatcccag tcaggccaat
     2761 cgcggcaact tccaggccgg cggtcgcagc agacgcagca gccagcgcat cccgggtagc
     2821 caggcatgct ccggtccagt aggcctcggg gtcgtgctcc acgcgtccgt cggaatacct
     2881 agacggggtg taggtataac gccccacgcc caggatctcg ccgtccgagc gcaccgcaac
     2941 agccttgatt acgctggttc cgatgtcgat cccgaggaac accgccatgg cctacgcctt
     3001 ctcctcattc atagtcatcc gcgcaaggat gaaggtgaac acgagtatcc ctataaggag
     3061 cagcaccgat actgcagtgg catatgaaaa ccgcgcgtag agaaacccgt gccggtaaat
     3121 gtggaaactc agtaactccg tgcggttgct tgggccgccg cttgtgagca cgtagactat
     3181 gtcgaaggtc
//
LOCUS       DAOTAC010000282         3186 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_23958_length_3186_cov_5.575854, whole genome shotgun sequence.
ACCESSION   DAOTAC010000282 DAOTAC010000000
VERSION     DAOTAC010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23958_length_3186_cov_5.575854"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="PLM74_10390"
     CDS             complement(<1..624)
                     /locus_tag="PLM74_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HON43516.1"
                     /translation="MVSGPILVVILLGAIAFIVWATGKARMNAFLVLLIAALAVGLLA
                     RKPPPVPTVNELGQSVPGLIDVIATGFGNTLASIGIVIVAGTIIGFILEKTGGALVLA
                     QAMLRLVGKSRSPLAMALAGYVVSIPVFCDSGFVILSPLNKALSQSSGISLGVLGTAL
                     SMGLYATHTMVPPTPGPIAAAGTLGADLGLVILFGLIASLPATIAGWI"
     gene            complement(745..2442)
                     /locus_tag="PLM74_10395"
     CDS             complement(745..2442)
                     /locus_tag="PLM74_10395"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HON43517.1"
                     /translation="MRSSGFFLRIALALLLLLSVGGAMAQEHEEVKWEPWGPRVDEIF
                     MPIKTNYSALISEFERGEIDIVPGLSASQWIDQLKANPNADVVTNYGHHVYYLCFNMR
                     REPLDADVVRQAMAHLVDRDRIITELLQSSVLPLASFLPPSSSFYKDDVPARSCDPAR
                     AAEILDQAGYKMDIATKIRRDPATRKPMREMSILAPSGSVGPSIAKLIANECRAIGLP
                     VNALSLNGNDMFSKVDKHEFDMYVAAYSVSRFPTSLTSFFHSSQRATGGYNKSGIKDP
                     QLDKALDEISYALDLQTAKEAADKAQVILAERQPWITIYSRPYIDAFRKDKFTGYVPM
                     HGEGAARNHWTLLGIRPTSGTAGVLRWPLTGEPATLNPCTYTLSYEAEVLSKITDSLM
                     AVDPITLEDIPWMAREWEISTWEPSKGKHGTVITWHLNDGILWQDGEPFTSGDIKFTI
                     EYLKKYKVPRYAGSVEDIVKVETPDRLTAKVYFSTESYWHLYNADLCFLPEHIWKSVW
                     NYKTFSPWRDRHPKVKDLTKLIGTGPFILKEFAPGKYVRLVKNPLYWRINPPDGAEN"
     gene            2702..>3186
                     /locus_tag="PLM74_10400"
     CDS             2702..>3186
                     /locus_tag="PLM74_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007952670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HON43518.1"
                     /translation="MGFKLTQKEQQLLSDQLNHEKVCIQKYSSYAQQTRDPQLRQMFN
                     QYASEEQNHYDTVSQLLSGQPANLSMGAGAGQGGQQGRFQQQRQTQQAQMQGGEAAEL
                     QGAVGGAGMTDQEEAAMLGDMLMTEKFVSGAYDTAVFESANSDVRQALQHIQREEQQH
                     G"
BASE COUNT          682 a          874 c          949 g          681 t
ORIGIN      
        1 gatccagccg gctatggtgg cgggcagcga tgcgatcagc ccgaaaagga ttacgagccc
       61 caagtcggcg ccgagggtgc ccgccgcggc tatgggcccg ggcgtcggcg gaaccatggt
      121 gtgcgtggcg taaaggccca ttgagagagc tgttcccagt acgcccagtg agatcccact
      181 tgactgcgat agggctttgt taagcggaga caagatgacg aagcctgagt cgcagaagac
      241 cgggatcgac accacatacc cggctagtgc cattgccaga ggcgatctcg atttcccgac
      301 cagcctgagc atggcctgtg cgagaaccag ggcgccgccg gtcttttcga ggatgaagcc
      361 tatgatcgtg cctgcgacga tcacgatccc gatgctcgcc agtgtattgc cgaatcccgt
      421 ggcgattacg tcgatcagcc caggaacgga ttgccccagt tcgttgacgg tgggaaccgg
      481 cggcggcttg cgtgccagga gccctaccgc aagagccgcg atcagcagca ccagaaacgc
      541 attcatccgt gccttcccgg tggcccacac aatgaaggct atggccccga ggaggatgac
      601 aaccaagatt ggtccgctaa ccatctttcc gacctcccgt gtctgtcgtt gcttaaccga
      661 gtcaggcaca aattggagtc gcgccctgcc caggcaggat cctttgtcat gtaggagttc
      721 ccaaagaagc gtcttgtatt tagcttaatt ctccgccccg tcaggcgggt tgatcctcca
      781 gtaaagtggg ttcttgacga gcctgacgta cttgccaggt gcgaattcct tcagaatgaa
      841 cgggcccgtg ccgatcagct tggtcaggtc cttgaccttg gggtgccgat cccgccaggg
      901 gctgaacgtc ttgtagttcc atacgctctt ccagatatgc tcaggaagga agcagagatc
      961 cgcgttgtac agatgccagt agctttcagt ggagaagtat accttggctg tgagacgatc
     1021 aggggtctcg acctttacga tgtcctccac cgaaccggcg tacctgggta ccttatattt
     1081 cttgagatac tcaatagtga acttgatgtc gccggacgtg aaaggttcgc cgtcttgcca
     1141 caggatgccg tcattcagat gccatgtgat cacagtgccg tgcttgcctt tcgaaggctc
     1201 ccaagtgctg atctcccact cgcgggccat ccagggtatg tcctcgagag tgatcgggtc
     1261 gactgccatc aggctatcag tgattttgct aaggacttct gcttcataac tgagcgtata
     1321 ggtacatggg ttgagtgtgg cgggttcgcc tgtgagcggc caacgtagca cacccgctgt
     1381 gcccgatgtc ggtctgatgc caaggagcgt ccaatggtta cgcgcggcgc cttcgccgtg
     1441 cattggcaca tacccggtga acttgtcttt ccggaaggcg tctatatatg gccgtgagta
     1501 tatggttatc cagggctgcc gctcagctag gattacctgc gccttatccg ccgcttcctt
     1561 ggcggtttgc agatcgagag cataggagat ctcatcgagc gccttatcaa gctgagggtc
     1621 tttgatacct gacttgttgt acccacctgt ggcgcgttgt gacgagtgga agaagcttgt
     1681 tagtgatgtg ggaaaccggc tcacgctgta cgcagccaca tacatgtcga actcatgctt
     1741 gtcgacctta gagaacatgt cgttgccatt cagagatagg gcgttaacag gcaggccgat
     1801 ggccctgcat tcgttcgcga tgagcttggc gatgcttggc ccgactgatc cagatggggc
     1861 gagtatactc atctctcgca tcggcttgcg ggtggcagga tcgcgccgaa tcttggtggc
     1921 gatgtccatc ttgtatcccg cttgatccag aatctcggcg gccctggccg gatcacatga
     1981 gcgtgcgggc acgtcgtcct tgtagaacga gcttgagggc ggaaggaagc tggcaagcgg
     2041 cagcacgctg ctctgtagaa gctcagtgat aatgcgatcc cgatccacta gatgcgccat
     2101 ggcttgacgc accacgtctg catcgagggg ttcgcgcctc atgttgaagc acagatagta
     2161 gacatggtgc ccgtagttgg tcacgacgtc ggcgtttggg ttcgccttga gctgatcgat
     2221 ccactgcgac gccgaaagac ccggcacgat gtcgatttca cctcgctcaa actcggagat
     2281 gagagcgctg tagtttgtct tgatgggcat gaagatctca tcgacgcgtg gaccccacgg
     2341 ttcccatttg acttcttcgt gttcctgtgc catcgctccg ccaacggaga ggagtagcaa
     2401 gagcgccagg gctatgcgga gaaagaatcc gcttgacctc attaccctac ctcctatcaa
     2461 ctggtgtttt ggcccgttgg gcatgcaggc tggaaagcat gcgctagacg ggcgcctacc
     2521 aggctatgcc tggctgtagc tggacaacac agagatatta attccacaaa cgtctcggtt
     2581 cacctttact gactgcataa ggcaacgaga ttgccgactg acttccgcgg ccggtttccc
     2641 gaactgacgt aacgcataaa ccagccgctc ccgaaccaca cttaagcggg aggtggatgg
     2701 tatgggattc aaacttacac agaaggaaca acagcttctg agcgaccagc tgaaccatga
     2761 gaaggtgtgc atccaaaaat acagcagcta tgcgcagcag acacgagacc cgcagctaag
     2821 gcagatgttc aaccaatacg cgtctgagga acagaaccac tacgatacgg tgagccagct
     2881 tctttccggg cagccggcca acctaagtat gggagcgggc gctggtcagg gcgggcagca
     2941 gggccggttc cagcagcagc gtcagaccca gcaggctcag atgcagggtg gggaagccgc
     3001 tgagctacag ggcgctgtgg gcggggccgg catgacggat caggaagaag cggcgatgct
     3061 gggagacatg ctgatgactg agaaattcgt ctcgggcgcc tacgatacgg ccgttttcga
     3121 gtctgccaac tccgatgtgc gccaggcact tcagcacatt cagagggaag aacagcagca
     3181 cggaga
//
LOCUS       DAOTAC010000283         3169 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_24105_length_3169_cov_4.164098, whole genome shotgun sequence.
ACCESSION   DAOTAC010000283 DAOTAC010000000
VERSION     DAOTAC010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24105_length_3169_cov_4.164098"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="PLM74_10405"
     CDS             complement(<1..479)
                     /locus_tag="PLM74_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43519.1"
                     /translation="MHVGCVDAGAAANDDADAMVDLRVNLGGIELANPVVMASGTFGY
                     GFDYEGLIEPETVGAVCVKGTTLLPRAGNKPPRIAETPCGMLNSIGLENPGIEAFIRE
                     HLPRLRERGATVIANIAGDTVEDYVKMAEMLEAAPGVAGIELNVSCPNVARGGMHFGV
                     "
     gene            complement(485..1387)
                     /locus_tag="PLM74_10410"
     CDS             complement(485..1387)
                     /locus_tag="PLM74_10410"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HON43520.1"
                     /translation="MPYLETADVLRQDCVAPGLYELELSAPQIAVAAVPGQFVHIRVR
                     PGQPLLRRPFSICDVGTNVGGSVTILYGVRGVGTSLLSSVQLGDRLDVMGPLGKGFTL
                     PESQSLSQSQLKSQLQSHPQQQSSRSPHALLVGGGLGIAPLVFLARMLAHNGHRATVF
                     YGANTACEMQAALLRLQASQEGSIQVFTTTLDGSAGHTGFVTDLLTAEPSASELADID
                     FIYTCGPEPMMVAVEQFATSRRIPGEMSLETALACGVGACLGCARRLKTDPGASPVYA
                     KVCKDGPVFSFGQVELNPVPCGEE"
     gene            complement(1488..2789)
                     /locus_tag="PLM74_10415"
     CDS             complement(1488..2789)
                     /locus_tag="PLM74_10415"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HON43521.1"
                     /translation="MRLLLKGGRVIDPANNIDAILDVLIEDGKIAAVGGIRHNEDMRA
                     DAVDCRGKIVCPGFIDLHAHLREPGYEYKEDISSGSHAAAAGGFTTICCMPNTDPVID
                     NGAVAGFVRRRAQEVGLVNVLPIGAITKKQAGEELAEMGELVSAGCVAVSDDGKPVLR
                     SDVMRHALEYSRLFGIPVLSHCEDASLSAGQMHEGYYSTVFGLKGIPAEAEEIMVTRD
                     LILARLTGGRLHICHISTKGALEAVKQAKASGLRVTCEATPHHLALTDEAVGSYDTDT
                     KVNPPLRSEEHVAALREALASGLIDCIATDHAPHHFESKDCEYGLASFGISGIETAVA
                     VIMDTLVHPGLLDIARMVELFTIGPAGVITANRATVGTLTCGAPADVTVIDPDLVRTV
                     DPRAFYSRGKNTPFKCMELKGWPCMTIVGGRIVAQDGLVVR"
     gene            complement(2773..>3169)
                     /locus_tag="PLM74_10420"
     CDS             complement(2773..>3169)
                     /locus_tag="PLM74_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HON43522.1"
                     /translation="GCEIRVAGPPTLIPPGLDRLGVKACFNLEDAIRGVDVINVLRIQ
                     RERQQTGLFPSLEEYSELYMLRPEHMSLAADDAIVLHPGPMNRGVEISSDIADGDTAL
                     INEQVTNGVAVRMALLFLMMGGRKDEIAA"
BASE COUNT          585 a          962 c          970 g          652 t
ORIGIN      
        1 acgccgaaat gcatgcctcc gcgggcaaca ttggggcagg agacgttcag ttcaatgcct
       61 gctacacctg gcgcggcttc gagcatttct gccatcttta cataatcctc aaccgtatcg
      121 ccggcgatgt tggcgatgac cgttgcgccg cgctcgcgca gccgaggtag gtgctcgcgg
      181 atgaatgctt cgatgcccgg gttttcaagc ccgatggagt tgagcatgcc gcagggggtt
      241 tcggcgatgc gaggcggctt gttgcctgcg cggggcagca gcgtggtgcc tttgacacat
      301 acagccccca ccgtctccgg ctcgataagg ccttcgtagt cgaacccgta tccgaatgtg
      361 cccgacgcca tcacaaccgg gttggcaagc tctatcccgc ccagattcac tcggagatcg
      421 accattgcgt cagcatcgtc atttgcagcg gcgcccgcat caacgcaacc tacatgcatc
      481 tgaactactc ctctccacag gggactgggt tcagctctac ctgcccgaac gagaagacgg
      541 gaccatcctt gcagaccttg gcatagacgg gactcgctcc ggggtccgtc ttgagacggc
      601 gtgcgcatcc taggcatgct cctactccgc aggccagggc ggtttcgagc gacatctctc
      661 cgggaatccg gcgtgacgtt gcgaactgct ctacagccac catcatcggc tccgggccgc
      721 aggtgtagat gaagtcgatg tcggcgagct ccgaggctga tggttccgcc gtcagcaagt
      781 cggtgacgaa tcccgtatgc cctgcgcttc cgtctagggt tgtggtgaag acctgtatgc
      841 tgccttcttg cgaggcctgc aatcgtagca gggcggcttg catttcgcag gcagtgtttg
      901 ccccataaaa gaccgttgcg cggtggccat tgtgcgccag catgcgggcc aggaaaacca
      961 gcggagcgat gcccaggccg cctccgacca gcagggcgtg cggcgatcga gaggactgct
     1021 gctgtgggtg cgattggagc tgtgacttga gctgcgactg tgatagcgat tgcgactccg
     1081 gcaatgtaaa acctttgccc aggggcccca tgacatcgag gcgatcccct agctgcacac
     1141 tggacagtag cgatgtgcca accccgcgca ctccgtaaag gatggttaca gacccgccga
     1201 cattggtgcc gacgtcgcat atgctgaacg gccgtctgag cagcggctga cccgggcgaa
     1261 cgcgaatatg cacaaactga cccggcacgg cggccacggc gatctgtggc gccgagagct
     1321 ccagctcata cagcccgggg gcaacacagt cctgccttag cacgtcggca gtctcaagat
     1381 agggcatgta atgtcctcca atctgccaga gcattctgct agtcttcctg tgagtcttcc
     1441 tgcgagtctt ccgccgggcc gcccagctgc gagccaggcc agctgagtta ccttactacg
     1501 agtccgtctt gtgccacaat tctgccgccg actatcgtca tgcatggcca gcccttgagc
     1561 tccatgcact taaacggcgt gttcttgccg cgcgagtaga acgcgcgcgg gtccacagtg
     1621 cgaaccagat cggggtcgat gacggtaaca tccgccggcg cgccgcaggt gagggtgcca
     1681 acggttgctc tgttggctgt gatgaccccg gccgggccaa tggtgaatag ctccaccatt
     1741 ctggctatgt cgagcaaccc tgggtggacc agtgtgtcca tgatgactgc gacggcggtc
     1801 tctatcccgg atattccgaa tgaggccagg ccgtactcgc agtctttgct ctcaaagtgg
     1861 tggggcgcgt ggtcggtggc aatgcagtct atcaggccgg aagccaacgc ttcgcgcagc
     1921 gccgctacat gttcctcaga gcgcaggggc ggattgactt tggtatcggt atcgtaggag
     1981 ccgacagcct cgtcggttag ggctaggtgg tgcggcgtgg cttcacacgt gacccggagg
     2041 cctgaggcct tggcctgctt gacggcctcc agtgcgccct tagtggatat gtggcatata
     2101 tgcagcctgc cgcctgtaag gcgcgcgagt atgagatcgc gcgtaaccat aatctcctca
     2161 gcctcagctg ggattccttt gagcccgaag actgtggagt aatacccctc gtgcatctgt
     2221 cctgcggaca ggctggcatc ttcacaatgg gacaggaccg gaataccgaa caggcgcgaa
     2281 tactccagcg catggcgcat tacatccgag cgcaggactg gtttgccgtc gtcggatact
     2341 gcaacgcagc ccgctgacac caactctccc atctcggcaa gctcctcgcc ggcctgcttc
     2401 ttggtgatgg cgccgatggg gagcacgttg accaggccga cctcctgcgc tcgccgtctc
     2461 acgaatccgg ccacggcgcc gttatcgatg actggatctg tgttgggcat gcaacagatt
     2521 gttgtgaaac cgccggcggc cgccgcgtgc gagccggagg agatgtcttc cttgtattcg
     2581 tagcccggct cacgcaggtg cgcgtgaaga tcgatgaaac cggggcatac aatcttgcca
     2641 cggcagtcta cagcatccgc gcgcatgtct tcattatgtc gaatgcctcc gacagctgcg
     2701 atcttgccgt cctcgatcag tacatccagt atggcgtcta tgttattagc ggggtcgata
     2761 acccttccgc ccttaagcag caatctcatc cttgcggcct cccatcatca ggaataggag
     2821 cgccatgcgc acggccactc cgttggtcac ctgttcgttt atgagcgctg tgtcgccgtc
     2881 cgcgatgtcg ctggagattt cgacccctct gttcatcggc ccaggatgca gcacgatggc
     2941 atcgtcggcg gccagactca tgtgttcggg acggagcatg tacagttccg agtattcctc
     3001 gagagagggg aagaggccgg tttgctggcg ctcgcgctgg atgcgcagga catttatgac
     3061 gtccacgccg cgaatagcgt cctcaaggtt gaagcaggct ttgacgccca ggcggtcgag
     3121 gccgggaggg atcagcgtgg gcggcccggc gacgcggatt tcgcagccg
//
LOCUS       DAOTAC010000284         3164 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_24151_length_3164_cov_4.571566, whole genome shotgun sequence.
ACCESSION   DAOTAC010000284 DAOTAC010000000
VERSION     DAOTAC010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24151_length_3164_cov_4.571566"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1752)
                     /gene="uvrA"
                     /locus_tag="PLM74_10425"
     CDS             complement(<1..1752)
                     /gene="uvrA"
                     /locus_tag="PLM74_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HON43523.1"
                     /translation="MSKDKIIIRGAREHNLKNIDVEIPRDQLVVVTGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLGQMNKPDVDYIEGLSPAISIDQKAASRNPRSTVAT
                     VTEIYDYMRLLYARIGIPHCPKCGREITQQSVDQIVDRIMNMSEGARIQVLGPVVRGK
                     KGEHRKVLDDLRREGFARVRVNGEARELTDQIDLERYKTHSIEAIVDRLVVRPGIQRR
                     LADSLETAMKLGDGLVLVDVVDSGDMVFSEKLACPDCGLSFGELQPRLFSFNSPYGAC
                     PACSGLGVNIEFDPALIFDAGQSIDDGAVLPYNRNGVISDFYIGRIREVSRVKGIDTA
                     VPWSSLPESARQMLEYGCGDERFRFRYRDSHGREQVWESKFEGVIPNLKRRYRETTSD
                     YIREDLERFMSSRPCAECCGARLKPEALAVTVGGRSIAEVSSMSTADALGFFEGLSLQ
                     LTERETLIARQVLKEIRARLGFLSNVGLDYLALDREAGTLSGGEAQRIRLATQIGSSL
                     VGVLYILDEPSIGLHQRDNARLIATLKELRDLGNTLIVVEHDEETIRSADYVIDIGPG
                     AGAHGGEVIAAGTVGEVQ"
     gene            complement(1749..>3164)
                     /locus_tag="PLM74_10430"
     CDS             complement(1749..>3164)
                     /locus_tag="PLM74_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016475436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HON43524.1"
                     /translation="ITGEMLGERESAFIYPATHWVTNQERMQAAIAAIEEELDARLSE
                     LKGAGKLLEAQRLEQRTRFDVEMMQEVGYCSGIENYSRHLAAREAGSAPATLLDYFPD
                     DYLMFIDESHVTVPQIRAMYNGDRSRKESLVEYGFRLPSAYDNRPLRFDEFTQRMNQV
                     IFVSATPAEWERSMSGQVVEQVIRPTGLVDPAVELRPVRGQVDDLIGQIRRTADQGYR
                     TLVTTLTKKMAEDLTDYLRELGIRVRYLHSEIDTLERIEILRGLRLGEFDVLVGINLL
                     REGLDLPEVGLVAILDADKEGFLRSATSLIQTIGRAARNVDGRVIMYADEVTGSMREA
                     IDETDRRRAIQIAHNKEWGITPESIKKAVRDLSEIQRAAGGEGIREGMRAVAVGGGGG
                     SGAGRGAALRGAALRGVGGPSDADAEALQEAEGMDERELARLIKNLEEQMKKAASLLE
                     FERAAEIRDRIRDLRRRFVQL"
BASE COUNT          603 a         1051 c          833 g          677 t
ORIGIN      
        1 ctggacttcg ccaacagtgc ccgccgctat cacctcgccg ccatgggcgc cggcacccgg
       61 gcctatgtcg atgacgtaat ctgccgacct gatggtctcc tcgtcgtgtt ccaccacaat
      121 gagtgtgttg cccaagtcgc gcagttcctt cagggtggcg attagtcgtg cattgtcgcg
      181 ctggtgcagc ccgatagatg gctcgtctag aatgtagagg acgcccacca ggctcgaccc
      241 gatctgcgta gccagcctga tccgctgagc ctcgccgcct gagagagtgc ccgcctcgcg
      301 gtcaagggca aggtagtcca accccacatt gctcaggaag cccaacctgg ctcgtatttc
      361 tttcaagacc tgcctggcta tgagcgtttc ccgctctgtg agctgcaggc tgagaccctc
      421 aaagaagccc agagcgtcgg ctgtggacat cgaggagacc tcggcaatgg atcttccgcc
      481 gacggtcacc gcgagcgcct ccggcttaag ccgtgcaccg cagcactcgg cgcacggtcg
      541 cgaactcatg aaacgctcca ggtcctccct gatgtaatcg gatgtcgttt cgcggtagcg
      601 ccgcttgagg ttgggaatca ccccttcgaa cttcgactcc caaacctgct cgcgcccgtg
      661 cgaatcacga taacgaaatc ggaagcgttc atcgccgcac ccatattcca gcatctggcg
      721 ggcgctttca ggcaaggatg accagggaac cgcggtgtct atccccttta cgcgggatac
      781 ttcacgtatc cggccgatgt agaagtcgga tatgaccccg tttcggttgt agggcagcac
      841 ggccccgtca tcaatagact ggccggcgtc gaaaatcagc gccgggtcga actcgatgtt
      901 cacgcccagg ccggagcagg ccgggcacgc cccgtaagga ctgttgaacg agaatagcct
      961 gggctgcagt tccccgaaac tgagcccgca gtcagggcag gccaacttct ccgaaaagac
     1021 catgtcgccg ctgtcgacca cgtccactag gaccaggccg tcgccgagtt tcatggccgt
     1081 ttccagcgaa tcggccagcc tgcgctgtat tccgggcctc acaaccagcc tgtcaacgat
     1141 agcctctatg ctgtgagttt tgtagcgctc caggtcgatc tggtccgtga gttcgcgcgc
     1201 ctctccgtta acccttacac gggcaaaccc ttcgcgccta agatcgtcga gcaccttcct
     1261 gtgctcgcct ttcttgcccc gaacaacggg gcccagcacc tgaatccgcg ccccctctga
     1321 catgttcata atcctgtcga cgatctgatc cacagactgc tgggtgatct cgcgaccgca
     1381 cttcgggcaa tgaggtatcc cgatacgtgc atacagcaac ctcatgtagt cgtagatctc
     1441 agtgaccgtg gccacggtgg aacgcgggtt tctgctggct gctttctgat ctatggaaat
     1501 cgcaggcgac aggccctcga tgtaatctac atccggtttg ttcatctgcc ccagaaactg
     1561 acgggcgtag gccgacagcg attccacgta tcgacgctgg ccctccgcgt atatcgtatc
     1621 gaaggcgagc gacgatttgc ccgaacccga caggccggtc actactacca gctggtcgcg
     1681 gggaatctcc acgtcgatat tcttgaggtt gtgctcacgc gcacctcgga tgatgatctt
     1741 gtctttgctc aaagctgtac gaacctccgc ctgagatctc tgatgcgatc ccgtatttcg
     1801 gccgcccgct cgaactcaag caggctggcc gccttcttca tctgctcttc gaggttcttg
     1861 atgagcctgg cgagctcgcg ctcatccatg ccttcggctt cctgcagagc ctccgcgtcc
     1921 gcatcgctag gcccacccac tccacgcaaa gccgccccac gcagagccgc tccgcgtcca
     1981 gctccggatc cgcctccgcc ccctacggcg acagctcgca tgccttcccg tatgccctcg
     2041 ccgccggcgg cgcgctggat ctcggatagg tcacgaacag ccttcttaat tgactcgggc
     2101 gtgatacccc actccttgtt gtgggctatc tgtatggcac gacgtcggtc agtctcgtct
     2161 atggcctcgc gcatagatcc tgtaacctca tcggcgtaca tgatgactcg cccatccaca
     2221 ttccgagcgg ccctacctat ggtctgaatg agagacgtcg ccgaccgcag aaacccttcc
     2281 ttgtcggcgt cgagtatggc caccagccca acctctggca gatccaggcc ctcgcgaagc
     2341 aggtttatgc ccaccagaac atcgaactcg cccaggcgca accccctaag tatctcgatc
     2401 cgttccagcg tatcaatctc ggagtgcaga tacctgactc tgatccccag ttcacggagg
     2461 taatcggtga ggtcctctgc catcttcttg gtgagcgttg tcaccaaggt tcggtatccc
     2521 tgatccgccg tacgacgaat ctggcctatc aagtcatcca cctgcccccg cacgggccta
     2581 agttcgaccg ccggatcgac caggcctgtt gggcgtatga cctgctccac cacttgcccg
     2641 ctcatagatc tttcccattc ggccggagta gccgaaacga agattacctg gttcatgcgc
     2701 tgcgtgaact cgtcgaaacg caagggccgg ttatcgtagg ccgagggcaa tctgaaacca
     2761 tattccacaa ggctctcttt tcgcgaacga tctccattgt acatcgcgcg tatctgcggc
     2821 acagtcacat gcgactcgtc aatgaacatt aggtaatcgt cggggaagta atcgagcaga
     2881 gttgcaggag cgctgcccgc ctctctggcg gccagatggc gcgagtagtt ctcgataccc
     2941 gagcagtacc ccacctcctg catcatctcc acatcgaacc gggtacgctg ctccagtcgc
     3001 tgcgcttcga gaagcttccc tgcacctttc agctccgaga gcctagcatc cagttcctcc
     3061 tcaatcgccg cgattgcggc ctgcattcgc tcctggttgg tcacccagtg agtcgcggga
     3121 tagatgaaag cagactccct ctcgcccagc atttcccctg ttat
//
LOCUS       DAOTAC010000285         3125 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_24500_length_3125_cov_4.149511, whole genome shotgun sequence.
ACCESSION   DAOTAC010000285 DAOTAC010000000
VERSION     DAOTAC010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24500_length_3125_cov_4.149511"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(277..2235)
                     /locus_tag="PLM74_10435"
     CDS             complement(277..2235)
                     /locus_tag="PLM74_10435"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HON43525.1"
                     /translation="MESRLGQRAQRVGSKIKAFVGGKAMIALATVAVSSLIIAYAVLP
                     QSVALEVGQFAKRDIESPRTIVNRVATDKLREEARKAYMKSAPNSLENYEISQSYSYM
                     AEESVDAVFESVATEREGDSDKTPQTVARAAAKKLGGAVDTLSLSAADLEGIAQIPKD
                     EFDSVRQVATSLVGRTMREIRITDASLQKVASGIPDDLVRAGLSPQWVKAVSRVVVGA
                     LRPNLSLDLAKVEKAAEEQARLVPPVYIQKGQSIIRRGDAATDEQIAVLSDLGLLGSR
                     GSIMGWTGVVALTAMAVAYTAAYVRRFTPGLSSAEGSLALCAVAGIVVLVSASVLVSL
                     VPDFAPYLIPAAFASMVISSLLSLEMAMAVNLSVAALIGIILRGDAFAMLAALAAGAA
                     GAYAVPGLRDRASMTRAVVFVGGAVAAVSVFYGLAFGEPQILSRWYWGFVNGMMSTVM
                     TVGSLPFFESIFGVTSPMRLLELSNPGHPLLRRLLLEAPGTYHHSILVGNLAETAAQA
                     VDADALLVRVGALYHDVGKLKRPYFFVENQFAQANPHDKISPALSTLVITSHAKDGVE
                     LAKQYKLPKSVVDLIQQHHGTGLVSFFYQRASENGRDQSVDEKDFRYPGPRPQTREAA
                     IVMLADSVEAAVRAQSDVSPWQVQGTVK"
     gene            complement(2244..>3125)
                     /locus_tag="PLM74_10440"
     CDS             complement(2244..>3125)
                     /locus_tag="PLM74_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HON43526.1"
                     /translation="MARANEVTVVGSQDVVEKVLRVLSDLQSAVRAGNRISGRETRYS
                     VMLAQDGRPETLSTVLADVIQVTARGKQIRPRTVGQKHYVDAIRNNDIVFGIGPAGTG
                     KTYLAMAMAIAALKSKQVSRIILTRPAVEAGEKLGFLPGDLQEKVDPYLRPLYDALFD
                     ILGLEVYERYRERGIIEIAPLAYMRGRTLDDAFIILDEAQNTSPEQMKMFLTRLGFSS
                     KAVVTGDITQIDLPRRRFSGLEEVRTVLVGIDGIEFVYFSDQDVVRHELVQRIIRAYE
                     RFDARRVGVSSDRREEE"
BASE COUNT          642 a         1047 c          813 g          623 t
ORIGIN      
        1 gtctgcctgt tcactatctc ggcggacatc tgtcgcctct ccttcccggt cttcccgctc
       61 tgcccggtcc ccattgctct ggtcttcgtg gtttgcctgg gtagcaggca cgaccggctg
      121 atccctgggc cccgaagctc ttaggtcggg gtattcgact cgctcatgat acactcccga
      181 cagcacatcg agaaaagcat tctcgatggc gtacagatcc ttgagcgtga ggtcacactc
      241 gtcaagctgg ccgtcggcga gtttgcccct gactacctac ttcacagtgc cctgcacctg
      301 ccacggcgac acgtctgact gggccctgac tgccgcctcc actgaatcgg ccagcatcac
      361 tatggcagcc tcgcgcgtct gcggacgcgg acccggatac ctgaagtcct tttcgtccac
      421 tgactgatcc cggccattct ccgaggcccg ctgatagaag aatgacacaa gccccgtgcc
      481 gtgatgctgc tggatcaggt caaccaccga cttgggcagc ttgtactgct tggccagttc
      541 cacaccgtct ttcgcatgag aagttatcac cagcgtcgac agagccgggc tgatcttgtc
      601 atgcgggttc gcctgcgcga actggttctc cacgaagaaa tacggccgtt tcagcttacc
      661 cacgtcatgg tagagcgctc ctactcttac tagcaaagcg tcggcgtcaa cagcctgagc
      721 agccgtttcg gccaagttgc ccactagtat agaatgatgg tacgtccccg gcgcctccag
      781 cagaaggcga cgaagaagcg gatgccccgg attggatagc tccagcaggc gcatggggga
      841 cgttacaccg aaaatagact cgaaaaacgg cagtgaaccc acagtcatta ccgtagacat
      901 cattccgttc acgaaacccc agtaccatct ggacagaatc tgcggttcgc cgaaggcaag
      961 accgtaaaag acagacaccg cggcgacggc gcctccgacg aacacaacag cccgtgtcat
     1021 gctggccctg tcgcgtagcc caggcactgc gtaagcgccg gctgccccag ccgccagagc
     1081 cgccagcatc gcaaaggcat cgccccgcaa tatgatgccg atcagcgctg ccactgacag
     1141 attgaccgcc atcgccatct ccagagacaa cagagacgag atgaccatcg acgcaaatgc
     1201 tgccggtatg aggtacggag cgaaatcggg cacgaggctc accaagacac tcgccgaaac
     1261 aaggaccact atgccggcta ctgcgcagag cgccaggctc ccttcggccg acgacagccc
     1321 aggggtgaac ctgcgtacat aggcggccgt atatgccacc gccatggccg taagcgcgac
     1381 cacgcctgtc cagcccatga tgcttccccg cgaacccaag aggcccagat ccgaaagaac
     1441 agctatctgc tcgtcagtcg cggcgtcgcc ccttctgatg atgctctgtc ctttctgaat
     1501 gtatacaggc ggaaccaggc gggcctgctc ttcggcagct ttctccacct ttgcaaggtc
     1561 tagcgagaga ttggggcgta gagccccaac gaccacccta gacaccgcct tcacccactg
     1621 aggcgacaac ccggcccgca ccaggtcgtc aggaatgcct gacgccacct tctgtaggct
     1681 ggcatcagtg atacggatct cgcgcatcgt gcgacctacc agcgacgttg caacctgccg
     1741 tactgaatcg aactcatctt tgggaatctg ggctatgccc tcaagatccg ccgcagacag
     1801 actgagagtg tcgaccgccc cgcccagctt cttggctgct gctctcgcca cggtctgagg
     1861 cgttttgtca gagtcgcctt cgcgctcggt tgccacggac tcgaatacag cgtccaccga
     1921 ttcctccgcc atatatgagt acgactggct tatctcgtag ttctcaagcg agttcggagc
     1981 gctcttcata taggccttgc gggcctcttc cctaagcttg tccgtggcca cgcggttcac
     2041 aatcgtcctg ggcgactcga tgtccctctt agcgaactgc ccgacctcga gagcaacaga
     2101 ttggggcagg acggcgtacg caatgatgag ggacgacact gccacagtag ccagcgctat
     2161 catcgctttg ccgcccacaa acgcctttat tttggaaccc acgcgctgcg cacgctggcc
     2221 taaccgcgac tccatgagca atgtcactcc tcttcgcgcc gatctgagga cacgccgacg
     2281 cgccgtgcat cgaatcgctc atatgcacgg atgatcctct gaaccaactc gtgccgtacc
     2341 acgtcttggt ctgaaaaata cacgaactcg atcccatcga tgcccacaag cacagtccgc
     2401 acttcctcca gccccgagaa acgtctcctc ggcaggtcta tctgggtaat gtcgccagtg
     2461 accaccgctt tggacgagaa ccccaacctg gtgaggaaca tcttcatctg ctcgggactg
     2521 gtgttctgcg cctcgtccag tattatgaag gcgtcatcca aagtcctgcc gcgcatgtac
     2581 gcaagcggcg ctatctcgat aatcccgcgc tctctgtagc gctcgtacac ctcgagcccg
     2641 agtatgtcga agagcgcgtc atatagcggc ctgaggtagg gatcaacctt ttcctgcagg
     2701 tcgcctggga gaaaccccag tttctcgccg gcctctaccg ccgggcgcgt cagtataatc
     2761 ctgctcacct gcttggactt gagggccgcg attgccatag ccatcgccag atacgtcttg
     2821 ccggtgcccg caggccctat cccgaacact atgtcgttgt tccggatggc atcgacgtaa
     2881 tgcttctgcc ccactgtgcg gggtcggatc tgcttgcccc ttgccgtcac ctgtattacg
     2941 tcggccagca ccgtcgaaag cgtctccggc cggccatcct gcgccaacat caccgaatac
     3001 ctcgtctccc tgccggaaat acgattgccc gcgcgcacag ccgactgcag atccgacagg
     3061 acacggagca ccttctccac tacatcctga gaccctacta cagtcacctc attggcacgg
     3121 gccat
//
LOCUS       DAOTAC010000286         3101 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_24738_length_3101_cov_4.635588, whole genome shotgun sequence.
ACCESSION   DAOTAC010000286 DAOTAC010000000
VERSION     DAOTAC010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24738_length_3101_cov_4.635588"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="PLM74_10445"
     CDS             <1..585
                     /locus_tag="PLM74_10445"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HON43527.1"
                     /translation="YRVRVAEAIYHGIIDYLVDAAGRNQLKPIDSADSDWGTVRKVAT
                     AQLTPDSAVLYFGGPTNFDDSLMPEVREVPGLSAMDATDALTAVMHCLIAGPGKDSIL
                     QPTIPRGTVLRWVRLSGDLASVNLSDEFVSNHWGGSRAEEMTVYSIVNTLTEFAYVKR
                     VRLLVEGKPIQSIAGHIDIEEPLVRNLSIVHFRS"
     gene            685..1974
                     /gene="murA"
                     /locus_tag="PLM74_10450"
     CDS             685..1974
                     /gene="murA"
                     /locus_tag="PLM74_10450"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HON43528.1"
                     /translation="MELVVAGGRRLSGSVAASGSKNATLAVMAGALLAEGETLIENVP
                     DIGDVGVMARTLQEIGVSVKRAGPRALRIRSRRVVHSEPPYELCKMLRASSLLLGPLL
                     ARNGRARVALPGGDQIGSRPMDLHVKGLRALGADVEVGHGYVATRAKSLVGARVYLDF
                     PSVGATENIMMAACLAHGTSIIENAAREPEVVDLANFLALMGAGIRGAGTDVIRIEGG
                     GPLSPVTYGVIPDRIEAATLLAAGIITYGNVRVTDVIPTHLESTLSKFGELGAEIEVG
                     AEHVEVSMRSPVRACDTKTLPYPGFPTDMQPQLGAVLSCAAGVSVITESVFENRFLHV
                     DELKRMGAVMRLEGRSLIVEGVPRLTGARVRATDLRAGAALVLAGLSAEGETVISGVE
                     HIARGHEHLCSKLAALGASIGSRDASFETAAGEDRSP"
     gene            1971..>3101
                     /gene="amrB"
                     /locus_tag="PLM74_10455"
     CDS             1971..>3101
                     /gene="amrB"
                     /locus_tag="PLM74_10455"
                     /inference="COORDINATES: protein motif:HMM:NF014899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HON43529.1"
                     /translation="MNGSSGSSGRLVLSALTPHPPIIVPEVGGPDTAQAQRTVDGMRR
                     LAQIFAEARPDTIVVISPHGPVFSDVIAITGLPRVEGDLRQFGAAVSMEFDNDLALAE
                     RIVSHAKLRGISAAMIEPSVISRLRAARRLDHGTMVPLYYISQAVSGFRVVPVAMGVD
                     TPDRLYMFGMALRDAVEEGEGRVALIASGDLSHCLTPGAPAGYSPKGKVFDERLVEAV
                     GRMDVGAIARLDDRLVEAAGECGLRPVFMMLGALDGLEVQAEVHSYEGPFGVGYAVAS
                     FRPVEASEDARRYDELLRGLEEKRAGRRRSESPYVALARQAVEEYVRHHKVVHVPEPL
                     PEGMDRAAGVFCSIHKGGQLRGCIGTTEPVRANIALEIVSNAI"
BASE COUNT          580 a          859 c         1066 g          596 t
ORIGIN      
        1 tatagagtgc gagtagctga agccatatac catggcatca tcgactatct tgtggatgcg
       61 gccgggcgga atcagctcaa gccaatcgat tctgctgact cagactgggg gaccgtacgg
      121 aaagtcgcta ctgctcagct cacgcccgat tcggcagtgc tctatttcgg cggacccacc
      181 aacttcgacg actcgctcat gcccgaggtg cgagaggtgc cgggcctgtc cgccatggac
      241 gcaacggatg cgctgacagc ggtcatgcat tgcctcatag cgggcccggg taaggacagc
      301 atactacagc ctacgatccc ccggggcact gtcttacgat gggtgaggct atcgggcgat
      361 ctggcttcag tcaacttgag cgatgagttc gtatcgaacc actggggcgg cagtcgcgcg
      421 gaggagatga ctgtatattc gatagtgaat accctgaccg agtttgccta cgtcaagcga
      481 gtgagacttt tagtcgaagg caagccaatc cagagtatag cagggcatat agacatcgaa
      541 gagccactcg tacggaacct gtcaatagtg catttccggt cctagaccca tcgaaaggat
      601 aaacggggct gtatgaagaa tacgtgcctt tgggcgcgca gcgcggctag gcctggatcc
      661 ggcgcgccgt ggaggtcaga tcaggtggag ctggttgtgg ccggcggaag gcgcctgtcg
      721 ggcagcgtcg ccgccagcgg gagcaagaac gcgacgcttg ccgtcatggc gggcgccctt
      781 ctcgcggagg gtgaaaccct tatcgagaac gtgcccgata tcggcgatgt gggcgtcatg
      841 gcacgtacac tgcaggaaat tggagtgagc gtgaagcgcg cgggccccag ggctctgcgc
      901 atacgctctc ggcgtgttgt ccacagcgag cccccgtacg agctgtgcaa gatgctcagg
      961 gcctccagcc tgctcctggg tccgttgctt gcgcgcaacg ggcgcgctcg cgtggcttta
     1021 cccgggggcg atcagatcgg gtcacgcccc atggacttgc atgtcaaggg ccttcgcgct
     1081 ttgggggctg atgttgaggt cgggcacggg tacgttgcaa ctcgcgcaaa aagccttgtc
     1141 ggcgccaggg tatacctgga ttttccgagc gtgggcgcga ctgagaacat catgatggct
     1201 gcgtgccttg cgcatgggac aagcataatc gaaaacgccg ccagagaacc cgaggtagtg
     1261 gatctcgcga atttcctggc cctgatggga gctggcataa ggggcgcggg caccgatgtg
     1321 atacgcatag agggtggtgg ccctctgtcg cccgtaacct acggcgtgat acctgaccgc
     1381 attgaggccg ccacattgct ggcagccggg atcatcacct atggaaacgt gagagtgacc
     1441 gatgtgatac ctacgcatct ggagtcgaca ctctccaaat tcggcgagct tggggcggag
     1501 atagaggtgg gggccgaaca tgtggaggtg tcaatgaggt cgcctgtccg ggcgtgcgac
     1561 accaaaacac taccctaccc gggattcccc accgacatgc agccgcagct tggagccgtg
     1621 ttgtcctgtg cagccggcgt aagcgtcatc accgagtcag tctttgagaa ccgcttcctg
     1681 catgtggacg agctcaaacg aatgggagct gtcatgcggc tggaaggacg gagcctgata
     1741 gttgaaggag tcccgcgcct caccggcgcg cgagtgcgcg ctacggatct tcgggcaggc
     1801 gcggctctgg tgctggcggg cctttcggcc gagggggaga cagttatttc cggtgtggaa
     1861 cacattgctc gcggccacga gcacttatgt tcgaaactgg cggcgctagg cgcctcgata
     1921 ggcagccgcg atgcgtcctt cgagactgct gcgggggagg atagatcgcc atgaatggtt
     1981 catcgggctc gtcagggcgg cttgtgctct cggccctgac gccgcacccg ccgatcatag
     2041 tgccggaggt gggagggcct gatacagcgc aggcccagcg cactgtggac ggcatgcgcc
     2101 gcctggcgca aatcttcgcc gaggcgcgcc ccgacacgat cgttgtgatc tcgccgcatg
     2161 gccccgtgtt ctcagacgtg atcgccataa ccggcctccc acgagtggag ggagatctga
     2221 ggcaattcgg cgcagcagta tcgatggagt tcgacaacga tctggcattg gccgaaagga
     2281 tcgtaagcca tgccaagctg cgtgggatat cggccgctat gatcgaaccc tcggtcatca
     2341 gccgcctacg ggcggcgcgc cgtctcgacc acggcacgat ggttcccctc tactacatat
     2401 cgcaggccgt gtccgggttt cgagtagttc ctgtggcgat gggcgtggat accccggacc
     2461 gactgtacat gtttgggatg gcgcttcggg acgcggtgga ggagggggag gggcgtgtgg
     2521 cgctcattgc cagtggcgat ctgtcgcatt gcctgacgcc aggcgctccg gccggctatt
     2581 cgccgaaagg caaggtcttc gacgaacgac tggtggaggc ggtgggccgc atggatgtgg
     2641 gggccattgc tcgcctggac gatcgcttgg tggaggcggc gggggagtgt ggcctgcgcc
     2701 cggtgttcat gatgttgggg gcgctggatg ggctggaggt gcaggcggag gttcattcat
     2761 acgaggggcc gttcggcgtg ggttacgccg tggcgtcgtt taggccggta gaggcttcgg
     2821 aggatgcgcg gcgctatgac gaactcctac ggggtctgga ggagaaacgt gctggcagga
     2881 gaaggtcgga gtcgccgtac gtcgccctcg ctcgacaagc agttgaggag tacgtgcgcc
     2941 accataaggt tgtgcatgtg cccgaacctc tgcccgaggg gatggatcgg gcggcgggag
     3001 tattctgctc catccacaag ggcggccagc tccgcgggtg cattggtacc accgaacccg
     3061 tgcgcgccaa catcgcccta gagattgtgt cgaacgcgat t
//
LOCUS       DAOTAC010000287         3093 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_24818_length_3093_cov_3.986833, whole genome shotgun sequence.
ACCESSION   DAOTAC010000287 DAOTAC010000000
VERSION     DAOTAC010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24818_length_3093_cov_3.986833"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="PLM74_10460"
     CDS             complement(<1..597)
                     /locus_tag="PLM74_10460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HON43530.1"
                     /translation="MKKSLLISLALVCVLTVSIGVYSASDLILATGGTAGTYYPLGGA
                     IANIANKRISKMNMAAQSTGASAENVRLINKGEADLALVQNDVADYAYNGTETFEGKP
                     IKTFGAIASLYPEVIQFVVRADGPIKTWADVRGKRISVGAPGSGTEANARQILDIYGM
                     TYADVEEQFLSFAESANQFKDGHIDGFFVTAGVPNPGIT"
     gene            1948..2721
                     /locus_tag="PLM74_10465"
     CDS             1948..2721
                     /locus_tag="PLM74_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON43531.1"
                     /translation="MLRIEGISKSYAKGSRRAVDDLTLHVEAGEIFGFLGPNGAGKTT
                     TIKMIVGLLTPDSGRISVDGRDVVQDPVGAKLGMGYVPDNPDLFDRLTGVEYLNFMGD
                     VYGVPAGERAKRIDSLLGMFELKGAVRDLVASYSHGMRQKLALTGALLSHPKLLVLDE
                     PMVGLDPKSSHLFKEMMNGHCEEGGTVFFSTHILEVAERLCHRVGIIDHGRLIACGTL
                     DDLKRQAALAGASGQADARRSQGKTLEELFLELTEGEDS"
     gene            2718..>3093
                     /locus_tag="PLM74_10470"
     CDS             2718..>3093
                     /locus_tag="PLM74_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43532.1"
                     /translation="MSGSGSISRSFRATQFFAVFRALLNVNFGISYGRQTFLVQKKQL
                     WEPILIAFGIGTALVTMMAGYIALLNAMYSNGEALGQGNVILTYAITAGQVLVLILGL
                     FWVIGGFYFSRDSSILTPLPLPA"
BASE COUNT          599 a          874 c          957 g          663 t
ORIGIN      
        1 ggttatccct gggttgggca cgccggccgt gacaaagaac ccatcgatgt gcccatcctt
       61 gaactggttg gcgctctcag cgaatgagag gaactgctcc tcaacatctg cgtaggtcat
      121 gccgtagatg tcgagaatct gacgggcgtt tgcctctgtt ccgctgccgg gagcgcccac
      181 agagatacgc ttgccgcgaa cgtcggccca ggtcttgatg gggccgtctg cacgcaccac
      241 gaactggatg acctcggggt acaacgaagc aatggcgcca aacgtcttga tgggcttgcc
      301 ttcgaaagtc tcggttccgt tgtaggcata gtccgctacg tcattctgca ccagagcgag
      361 atctgcttcg cccttattga tcaacctaac gttctcagca gatgctccgg tggactgggc
      421 agccatgttc atcttggaaa ttcgcttgtt tgcgatgttg gcgatggccc cgccgagcgg
      481 atagtaggtg ccggcagtgc ctcccgtggc tagtatcagg tcactcgcgg aatagacgcc
      541 gatcgagacg gttaggacac acaccaaagc cagggaaatt agaagagact tcttcaaatc
      601 ggaaccctcc tcgtaaatat gtgtggcgag cccattgcga taccgtgcag gcagcgcctc
      661 aaacgtgaaa gcataattct ccactgaatg tcctcatacc tgcaagctgc ggaagaattc
      721 ttgtcgtctg aggagatatg atacaacggc gggaggccca ggggcttgcc agatccaggt
      781 ttcgggggtt tcgccttcgc gtttgcgcag gactttcatt tccccccccg ccccgtgagg
      841 cacgtgcccg ggccgagaga cggtggccga cgcagcggca tgtgccctgc ccgagaaacg
      901 gtggcctacg cggcgtggcc ggcctcaggg gtgcgcgaca tcggcggtga tcgcgtcctg
      961 tgccctacgg gacacctttt ggggccatcc ctagatggaa gggattctgc gtgcacttat
     1021 cgcccacggt cctcgccaca cgcccacttg ccaacaaaac gagcactttg cgcggaagac
     1081 tcgatgcccg ggctacttct gcgaggcgct tccggagatc ggtccggtcg gacgtcaggg
     1141 tggccggcgc tgcgggatac cctcggaggg tatccgggaa gcttttttcc gccttggagg
     1201 cgttcgacgg agattttctg cacaaatgcg gcgcaaaatt tccgcgcaag agacccttgc
     1261 ttacttatgc tgggaatacc gcgggaacaa acgacattca gaagcgaggg aggcgtcccc
     1321 ggatggtagt aaacgaactt aatgtatggt actggtggcc ttttgctatt taagggcttg
     1381 cagtgacgcc ggggcttcct cgctatggca cgcaagtggc ccgaaaccag tgcctcggaa
     1441 attttcttca cctacgcggc aggaaatttc cgctttgcgc cgtggaaact gcaaatctct
     1501 gcaggcccag ctaaaggtgc ccaagcgcta gagcctccaa gagccagcgg cctcaggctc
     1561 agggcatccc tgtgcgacgt ctgttttcct gtcgagaccg cgcatcacgc ggttgcaata
     1621 gaccacctga cctgcaccgg agcggcaata gacggtagtc tctgtcctct catctgccgg
     1681 cggcatccta tctcatcagc gcttccatct gtgcgcgtgt ccgcctggcg agtgtccgcc
     1741 cggcgagcgt ccgcccgacg cgtccgcccg atgcgtgtcc gcctggcgag tgtccgcccg
     1801 atgcgtccgc ccgacgcgtc gatgggaaga tagaaatgcc gggccctgag aagcataatc
     1861 gttgcacccg cgttccagac ggacgatgtt tgacactccc agaatgcgag tatacacttt
     1921 tcataactgc agcgaggagg ttcgagcatg cttcggattg aggggatctc gaagagctac
     1981 gcgaagggat ctcggcgggc ggtcgatgat ctcactttgc atgtggaggc gggggagatt
     2041 ttcggttttc tcgggccgaa tggcgcgggc aagactacga ctatcaagat gatcgtcggc
     2101 cttctgacgc ccgattcggg caggatttcc gtggatggtc gcgatgtcgt gcaggaccct
     2161 gtcggggcga aactcgggat ggggtatgtt cccgacaacc ccgatctctt tgaccggctc
     2221 accggagtgg aatacctgaa cttcatgggg gatgtctacg gcgttcccgc tggggagagg
     2281 gcgaagcgga tcgacagcct tctaggaatg ttcgagctga agggcgctgt tcgcgacctg
     2341 gtagctagct actcccatgg aatgaggcag aagcttgcgc tcacgggcgc gctgctttct
     2401 catccgaagc tcttggtgct tgacgaaccc atggtgggtc tggacccaaa gagctctcat
     2461 ctgtttaagg agatgatgaa cgggcactgc gaggagggag gaacggtctt cttctctacc
     2521 cacatcctcg aggtggccga gaggctatgc cacagggtgg gcataatcga ccatggtaga
     2581 ctcatcgcct gcggcacgct ggatgacctg aagcgccagg cagcgctcgc cggggcgtcg
     2641 gggcaggcgg atgcgcggcg ttctcagggc aagactttgg aggaactctt cctcgagctg
     2701 acggagggcg aggactcatg agcggatcgg gcagcatctc gcgatctttt cgggcgacgc
     2761 agttcttcgc cgtgtttcgg gcgctgctca acgtgaattt cggtatctca tatggccgcc
     2821 agaccttcct tgtacagaag aagcaactat gggaaccgat actgattgcg tttgggatcg
     2881 gcacggcgct agtgacgatg atggccgggt atatcgcgct actcaatgcc atgtactcta
     2941 acggcgaagc cctgggccag gggaatgtga tcctaacata tgcaatcacg gccgggcagg
     3001 ttctggtgct catactgggg ctcttttggg tgatcggggg gttctacttc tcgcgcgatt
     3061 cgtccatact cactccgctg cctctgccgg cgt
//
LOCUS       DAOTAC010000288         3087 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_24880_length_3087_cov_8.549142, whole genome shotgun sequence.
ACCESSION   DAOTAC010000288 DAOTAC010000000
VERSION     DAOTAC010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24880_length_3087_cov_8.549142"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..106)
                     /locus_tag="PLM74_10475"
     CDS             complement(<1..106)
                     /locus_tag="PLM74_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015991113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HON43533.1"
                     /translation="MRVGSLFSGIGGLELGLERAGMKVIWQVEFDPFCQ"
     gene            complement(97..468)
                     /locus_tag="PLM74_10480"
     CDS             complement(97..468)
                     /locus_tag="PLM74_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43534.1"
                     /translation="MTDTQTEQLTGRALDAAVAEHVIGAGSSAAWDYSARIEPALHAM
                     RHILSRGAWAQNMFYAALWGQAPAAGPARWKFEHTLTALILEDKLPEAICRAAVYVAM
                     QPQWMGSNDSNQCESSDGKCE"
     gene            complement(622..1980)
                     /gene="dnaB"
                     /locus_tag="PLM74_10485"
     CDS             complement(622..1980)
                     /gene="dnaB"
                     /locus_tag="PLM74_10485"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HON43535.1"
                     /translation="MADRVPPYDKEAEEAALGSALQSNEAADMLIEIVPGEEAFYFEP
                     HRMVYAAVSALRRENKPCDLVTLTSTLRDAGRLEQVGGAHFISYLGNSVPTASHVRHY
                     ASIVREKALLRRVIAICTNAVTSAYDGDLDCITALEAELMNLQRATPMAEAEPYRQVV
                     SRVMDILGQRVQAQSPTTGVPTGFEELDRMTSGLQPGELAIIGARPSMGKSALARAIA
                     ENAAQKGHPVLFAALEDTPENMVQRSLAGRAQVDLWNLRQGRINDGEWRKLGYQLNRL
                     AELPVWPLGPSGVSIQAIRRAIRKVVAAEGKLGLIVVDYIQLLTPPRRRDNRYLEIAE
                     TSQALKTIAQEYRVPMLAVAQLNRGVEARRNKRPQLSDLRESGALEQDGDLVMLLYRA
                     DYYSTKARPGITELIIAKQRNGPVGTVHLYFDRTTTRFLEIDKDTPPEGSDDDDGDDD
                     GE"
     gene            complement(1993..2925)
                     /locus_tag="PLM74_10490"
     CDS             complement(1993..2925)
                     /locus_tag="PLM74_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43536.1"
                     /translation="MPNRFLKESITTSDTLPLVSMEAEVLFYRLIVKVDDFGRYHGEP
                     HLILAQALSNRIAQVSQDDVEHWLSELAAAGLLRRYRSNGRPYIELATFTEHNSPRAA
                     ASRFPDPPADDNDCEQVQTVACNCSQTQADANRCEQPKADASKCSQGPTDANVREQPK
                     ANVSVFDKRIRSTESVRARAREVAADPEVTEKFTRFMQTYPKRADDGQAWEEWCVNVA
                     VGASPDNMIKAAGHYAAMTRERKTMPRWIKNPATFLRESWHRFVDGIPENENDRSDPR
                     GRDRPDDDGADDGESSPVPGYDATRKYLQAKMSG"
BASE COUNT          504 a         1012 c          876 g          695 t
ORIGIN      
        1 gctggcagaa cgggtcgaac tcgacctgcc agattacctt catgccagcg cgttcaagcc
       61 ccagttctag tccgccgata cccgaaaaca agctacctac tcgcatttgc cgtccgatga
      121 ttcgcattga ttcgaatcat tgcttcccat ccactgcggc tgcatcgcca cgtataccgc
      181 cgccctgcat atcgcctcgg gcagcttgtc ctccagaatg agcgcagtca gcgtatgctc
      241 gaacttccag cgcgccggtc ccgccgcggg cgcttggccc cagagagcgg catagaacat
      301 gttttgcgcc catgcgccgc gcgagaggat gtggcgcatg gcgtgtaatg caggctcaat
      361 gcgagcgctg tagtcccatg cggcgcttga ccccgcgcca attacgtgct ccgcgaccgc
      421 cgcgtccagc gcgcggccgg tgagctgctc tgtctgcgtg tctgtcatcg cgatttcacc
      481 ccgtatctct tgcatatctc cgcaacccta tgctgcagct catggtcgcc tatctcaagc
      541 cttcgccatg cccgacaagc ctcgcggcac tcctcggccg caagacgccg gcgcttgggc
      601 gcggggagtc gtcgggtcgg ctcattcgcc gtcatcgtcg ccgtcatcgt cgtcactccc
      661 ttcaggaggc gtgtccttgt ctatctcaag gaaacgcgtg gtagtcctgt cgaagtaaag
      721 gtgtactgtg ccgaccgggc catttctctg cttggctatg atcaactcag tgatgccggg
      781 cctcgcctta gtgctgtagt agtcagcccg gtatagcagc atcactaggt cgccgtcctg
      841 ctctagggcg ccgctttcgc gcaagtcaga tagttgcgga cgcttgttcc gtcgggcttc
      901 aacgcccctg ttgagctgag ctacggccaa catgggcacc cggtattcct gtgctatcgt
      961 cttgagcgcc tgactggtct ctgcgatctc cagatagcgg ttgtctcgtc tccgcggagg
     1021 cgtcagcagc tggatatagt ccaccacgat cagtccgagc ttgccctcgg ccgcgaccac
     1081 cttccggatg gcccgtctga tggcctgaat cgacactccg ctgggcccga gcggccagac
     1141 gggcagttct gccaggcggt ttagctggta gcccagtttg cgccactcgc catcgttgat
     1201 tcgtccctgt ctcaaattcc ataggtcgac ctgggcgcgg ccggccaaag acctctgcac
     1261 catgttctca ggcgtgtctt caagcgcagc gaacaacaca ggatggccct tttgggccgc
     1321 attctcggca atggccctag ccagcgcgct cttgcccatg ctgggcctgg ccccgatgat
     1381 cgctagttcg cctggctgga gacccgacgt catgcggtcc aactcctcaa accccgtggg
     1441 cactcctgtt gtcggactct gcgcctggac gcgctggcca aggatgtcca ttactctgct
     1501 gaccacctgg cgatatggct ctgcctctgc catgggcgtc gcgcgctgaa ggttcatcag
     1561 ctccgcttcc agagctgtta tgcagtccag atctccatcg taggcgctgg tcacagcgtt
     1621 cgtgcatatc gcaatcaccc ggcggagtag ggctttttcg cgcactatgg acgcataatg
     1681 gcgcacgtgc gaggcggtcg gcacggaatt tcccaggtag ctgatgaagt gggcaccgcc
     1741 gacctgttcg agcctacctg catcgcggag cgtcgacgtc agcgtaacaa gatcgcaggg
     1801 tttgttctct cgccttagcg cggacacagc tgcatagacc atccggtgcg gctcgaagta
     1861 gaacgcttct tcgcctggca cgatctcgat cagcatgtcg gcggcctcgt tcgactgcag
     1921 ggccgaaccc agggcggctt cttcggcttc tttgtcgtat ggcgggactc tgtctgccat
     1981 ggctccgcac ctctatccgc tcatcttggc ctggagatat ttcctggtcg catcgtagcc
     2041 gggcaccggg gaggactcgc catcatcagc gccgtcgtcg tcgggccgat ctcggcctcg
     2101 cggatcgctt ctgtcgttct cattctcagg gatgccgtcg acgaaccggt gccacgattc
     2161 gcgcaggaag gtggcggggt tcttaatcca cctgggcatc gtcttgcgct cccgcgtcat
     2221 ggcggcgtaa tggcccgcgg ccttgatcat attgtcggga ctggcgccta cggcaacgtt
     2281 gacgcaccac tcttcccagg cctggccgtc gtcggccctc ttggggtagg tctgcatgaa
     2341 ccgagtgaac ttctccgtta cttcggggtc agcggcgacc tcgcgcgcgc gcgcgcgtac
     2401 actctctgta ctacgaatac gtttatcgaa tacggagaca tttgctttcg gttgctcccg
     2461 aacgtttgca tctgttggcc cctgcgaaca cttgcttgca tctgctttcg gttgctcgca
     2521 tcggttggca tctgcttgcg tctgcgagca attgcatgca actgtttgca cctgctcaca
     2581 gtcgttatca tctgcagggg gatccggaaa acggcttgca gccgcacgcg gcgagttgtg
     2641 ctcggtgaac gtggccagct cgatatacgg acgcccgttg ctccggtatc gtcgcaagag
     2701 ccctgccgct gccaactcag acagccagtg ctccacgtcg tcctgactga cctgcgcgat
     2761 gcggttggag agcgcctgcg ccagaatcag gtgaggttcg ccgtggtatc ggccgaagtc
     2821 gtcgactttg acgatcagcc tatagaacaa cacctcggcc tccatgctca ccagaggcag
     2881 cgtgtcgctg gttgttatcg actccttcag aaacctgtta ggcatcgttc actcctccag
     2941 attggttctc tctcacgcgc tctccccgcc gaacaaatcc aacaccatct gcctccccgg
     3001 cacgtgctgc ccaaacgccc tgtcgagcgc ccgcgccgta atgccgagct cccggatcct
     3061 cgagtacagc tgcgcctggc actcctg
//
LOCUS       DAOTAC010000289         3072 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_25024_length_3072_cov_4.564799, whole genome shotgun sequence.
ACCESSION   DAOTAC010000289 DAOTAC010000000
VERSION     DAOTAC010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25024_length_3072_cov_4.564799"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="PLM74_10495"
     CDS             complement(<1..842)
                     /locus_tag="PLM74_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43537.1"
                     /translation="MGNMRCACRTAAALVLILLAAATSGCWDALDIDRRAFIAVMGLD
                     TDVSEQGRRPRVSVMIPAMGGLGGGAGGGAGAGEETSSKVLVVEESGVNVEEAVQRIR
                     SMVGGVIDFSTLQYVVTSKDASPEDLTEALHAIQFTRFIPLAPLVFATEQKAGDLLRA
                     QSPTGRSFTDTLDAIQIQSRLGFGHIQYPGAWETMALLINKTGDMALTLVNPTDEGDG
                     LKAVGLAVYDGPKYVGTVAPADALMLHCVARRKYPGGAYLFRGDDRDLLIRIVRARTR
                     VEVGP"
     gene            complement(844..1959)
                     /locus_tag="PLM74_10500"
     CDS             complement(844..1959)
                     /locus_tag="PLM74_10500"
                     /inference="COORDINATES: protein motif:HMM:NF015783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GerAB/ArcD/ProY family transporter"
                     /protein_id="HON43538.1"
                     /translation="MREPYLSWPEAAWLIMQYTICANYLFMPQLLYSMSGRLSWAMPI
                     IAMLPAAAGVWATYYLHTRFPQERLGQFLPRLIGRPLSIVVGGAFILFWLGGAVTNVT
                     VFAHYVSDTAMQSTPVTALVAASALIIVLVSLSGLKTLVRVTDALLYLLAPFLLFALF
                     WPFFGSRIDFSNLAPIRLSDLTADKWRLAGAAAAMYHGYQLIFIAGPALNPPGRSALA
                     AGIAGTLAAVTAFLSFVAMPILAFGWPYANLLTYPAASFLEVVAPRTGLFPIRRFDFA
                     LTVLYRNIMIVAAMSYFYSAAQTVTDLVSPSARRIHPWFVIGLGIAMVVSSAAFKGIR
                     AVMQFTGVWILLGALTTTLTLILAIVARFRASVSGVR"
     gene            complement(1952..>3072)
                     /locus_tag="PLM74_10505"
     CDS             complement(1952..>3072)
                     /locus_tag="PLM74_10505"
                     /inference="COORDINATES: protein motif:HMM:NF015292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HON43539.1"
                     /translation="LDLRGRVGRQVSPPISEPSVRGPRDSFTENLFDNLSLVRQRIRS
                     RDLRVRELTAGLDTRTRVAVCYLEGRSSPEVVDAVIQRLSAVTAAAVIDSSYLVPYLS
                     KRTWSLFPPAAATERADRACAAIVQGRIVVICDNSPFALVIPIHLVTLFQANEDHYNL
                     AIHSFLLRSVRVIGWLAATLAPALYIGIASFNPGILPAHAVMTIASARQGVPYPPVIE
                     VLIMDIALEMLAEATVRLPTYVGGAATVVGGLILGTAAAQAKLVSNVMIIVVALTAIG
                     SFAIPDYQNQLSWRTVRYLFTFSAAILGIFGVSTAGLIILTHTCSIDVLGESFMSPIA
                     PWRWTSMAKDVPMRVPEPLAVKLTQRQREEEAEGTADA"
BASE COUNT          673 a          933 c          891 g          575 t
ORIGIN      
        1 ggaccgacct cgacccgggt cctcgcccgg acgatgcgaa tcagcaggtc acggtcgtct
       61 ccgcgaaaca gatatgcgcc tccaggatac ttgcggcgcg ccacgcagtg aagcatcagc
      121 gcgtcggcgg gtgccaccgt ccccacgtat ttcgggccgt catatacggc caagcccacc
      181 gccttcagcc cgtctccttc gtctgttggg ttgactagcg tcaacgccat gtctcccgtc
      241 ttgttgatga gcagcgccat ggtttcccat gccccagggt attggatatg gccaaacccc
      301 agccggctct ggatctgaat ggcatcgagc gtatctgtga aacttctgcc tgtaggggac
      361 tgggctcgaa gtagatcgcc ggctttctgc tcagtagcga agacgagagg ggccagcgga
      421 atgaagcgtg tgaactgtat cgcatgcaag gcttctgtca ggtcctccgg gcttgcgtcc
      481 ttgctggtta ccacgtattg aagtgttgag aagtcgatca cgcctccgac catagaacgt
      541 attctctgca cagcctcctc cacattgacc ccgctctcct ccaccaccag caccttggag
      601 gacgtctcct cccctgcccc ggcgccgccc ccggcgcccc cgcccagccc gcccattgcg
      661 gggatcatca ccgacacacg ggggcgacgg ccctgttcag atacgtccgt gtctagtccc
      721 atgacggcta tgaaggcgcg cctatcgatg tcaagcgcgt cccaacatcc cgaggtcgcc
      781 gccgccagga gtatcagtac taatgcagcg gccgtccggc atgcgcagcg catgttgccc
      841 atctcagcgc accccggaca cgctggcccg aaagcgtgcg actatggcca gaatgagggt
      901 cagcgtggtc gtcagggccc caagcaatat ccatacgcct gtgaactgca tcaccgcccg
      961 gatccccttg aaggcggcgg aagagacaac catggctatg cccagtccga tcacgaacca
     1021 agggtgtatc cgccgcgccg acggcgacac caagtcggtc acggtctgcg cagcgctgta
     1081 gaaataggac atcgctgcaa ctatcatgat gttcctgtac agcacggtca gcgcgaagtc
     1141 gaacctgcgt attgggaaca gcccagttct gggagctact acctccagga acgatgctgc
     1201 gggataggtc agaaggttag cgtacggcca tccaaacgcc aggatcggca tcgccacgaa
     1261 cgacaagaat gcggtaacgg ctgccagtgt tcccgcgatt ccagccgcaa gcgcgctacg
     1321 acccggcgga ttcagagctg gccctgcaat gaagataagc tgatagccat ggtacattgc
     1381 tgccgcagct ccagccagtc gccacttgtc tgctgtcaga tcagacagcc ttatcggcgc
     1441 caggttggaa aagtcgattc tgcttccaaa gaatggccaa aacagggcaa acagcagaaa
     1501 aggcgccagg aggtacaaga gagcgtcagt aacgcgtacc aatgttttca ggcctgacag
     1561 tgaaaccaac acgatgatta gcgcgcttgc cgcaacgagc gcggtcacag gggtggactg
     1621 catcgctgtg tcggacacgt agtgcgcgaa cacggtcaca ttggtcactg cgccgcccaa
     1681 ccagaaaagg atgaacgctc cgccgacaac tatcgagagc ggccgcccga tcagccgcgg
     1741 caggaactgt cccagacgct cttgggggaa tctagtgtgc aagtagtacg ttgcccacac
     1801 gcccgccgca gccggtaaca tggcaattat tggcattgcc catgaaagcc ggccggacat
     1861 cgagtacaac agctgaggca tgaacaggta gttggcgcag atggtatact gcatgatcag
     1921 ccatgctgcc tcaggccagc tcaggtaagg ctcacgcatc tgccgtgccc tccgcctctt
     1981 cttcccgctg ccgttgggtc agtttcaccg ctagcggttc aggcacgcgc ataggtacat
     2041 ccttcgccat agacgtccac ctccacggtg ctatgggcga catgaaggac tcgccgagca
     2101 cgtcgatgga gcaggtgtgg gtgaggatta tgagccctgc agtgctcact ccgaatatcc
     2161 ccaatatcgc agcagagaac gtgaataagt acctgaccgt ccgccatgaa agctgatttt
     2221 gatagtcggg aatcgcgaac gagccaatgg cagtgagtgc cactactatg atcatcacat
     2281 tgctcaccag cttggcttgg gcagcggctg tgcccagtat caggccgcct acaacggttg
     2341 cggcgccgcc aacatacgtg ggaagcctta cggttgcctc agccagcatc tcaagggcga
     2401 tgtccatgat cagaacttcg attacgggag gatatggcac tccctgcctg gccgatgcga
     2461 tggtcattac ggcgtgggca ggaagtatcc cggggttaaa tgaggcgatg ccgatgtaga
     2521 gcgccggcgc cagtgttgcg gccagccaac cgatgacgcg aacacttctg agaagaaaag
     2581 agtgtatggc gaggttgtaa tgatcctcat ttgcctgaaa gagcgtgacc agatgaattg
     2641 gaattacgag ggcaaacggc gagttatcgc atatcaccac tatgcgcccc tgtacgatcg
     2701 cggcgcacgc gcgatcggcc cgctcggttg cagcagccgg cggaaagagc gaccacgtcc
     2761 ttttcgaaag atacggcacc aggtatgagc tgtcgattac agcggcggca gtgacggcag
     2821 aaagacgctg gatcacagcg tctactactt ccggggaaga cctgccctca agataacata
     2881 cggccaccct ggtacgggta tcaaggcccg cggtcagctc gcggaccctg aggtcgcgac
     2941 tccttatgcg ttgccttacg agggagaggt tatcgaacag gttttcggtg aagctatccc
     3001 gcggccccct caccgacggc tcggatattg gggggctgac ctggcggccg accctacctc
     3061 tcaggtcaag cg
//
LOCUS       DAOTAC010000290         3070 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_25045_length_3070_cov_4.586401, whole genome shotgun sequence.
ACCESSION   DAOTAC010000290 DAOTAC010000000
VERSION     DAOTAC010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25045_length_3070_cov_4.586401"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="PLM74_10510"
     CDS             complement(<1..462)
                     /locus_tag="PLM74_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43540.1"
                     /translation="MDYELILLDADGTLFDYDRAERHAIETTFRDFGLDYSDSILAKY
                     RVVNDALWKELERGNTTSAELRVERFRRLLAEIREAAPERGSTQASTHTSARASAQTP
                     ASEIAPVPAAVPAPDAATLSRRYLHHLARGSHLIDGAEDICRYMAQRYTLAI"
     gene            728..1567
                     /locus_tag="PLM74_10515"
     CDS             728..1567
                     /locus_tag="PLM74_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014185069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HON43541.1"
                     /translation="MFRIGEFSKLTQVSVRMLRHYDEVGLLRPAQIDEATGYRLYSVQ
                     QIPAVQKIVLLRNVGFGIAEIAAALADWDRQSITQRLVNKAREIEEAIRLEREHAARI
                     AAAIDDLQTGRIAVHYNVTIRSIPGYQILSLRRTIPNYHCEAVLWDELCAFVSRERVE
                     LHPRGGAIAFFHDDEHRDSDVDVEVGVIVSKPGDNKDGFIYRETEKVDTMACIMVYGP
                     FENIGPSYQSFAYWLETHQHYAMHGPVRQICHRGPFNAQNSDDYLTEVQVPVTKRLRD
                     AVP"
     gene            1644..2117
                     /locus_tag="PLM74_10520"
     CDS             1644..2117
                     /locus_tag="PLM74_10520"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005813293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmO family methyltransferase"
                     /protein_id="HON43542.1"
                     /translation="MEQLYVKPIGRIVINENGMFVKVEPQYTPALKELDGFSHLNIFW
                     WFSNLDNDVSRSVLEVDRPYRTSPAVLGTFATRSPQRPNPIALTVAQIIHIDHKNGMI
                     QVGYTDADNGSPVIDLKPYTPSLDRVENPSVPEWCRHWPKSLEKSADFDWDSEMN"
     gene            complement(2367..2951)
                     /locus_tag="PLM74_10525"
     CDS             complement(2367..2951)
                     /locus_tag="PLM74_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43543.1"
                     /translation="MSTEIGEDITDQRLMIVILVVLPHGVVGGACTSVSFGSAKLKAL
                     ADAPRSRSPLDAEKQHAQEMITGEVRVRCSLFANWEVLRMSCGDKSAIPRGRMKKTDQ
                     TKGSGCTIPDSPGVYRHVNKRTGEVEYVGQTENLRVRQQQHVRSGKLDPEKQWVEYSE
                     AKASATKDDLRNTERQHVERHDPSGNIYGGGNGR"
BASE COUNT          669 a          905 c          866 g          630 t
ORIGIN      
        1 gatagcaagg gtgtaccttt gggccatgta gcggcagata tcctcggccc cgtctatcag
       61 atgcgatcct ctggccaggt gatgaaggta acgccggctc agggtcgcag catcgggagc
      121 gggaaccgca gcaggaacag gagcgatctc ggaggcggga gtctgagcag aagcacgagc
      181 agaggtatga gtagaggcct gagtagaacc gcgctccggc gcggcctcgc ggatttcggc
      241 aaggagacgt ctgaaacgct caacgcgcag ctccgcagag gtggtatttc ccctctcaag
      301 ctctttccag agtgcgtcat tcactacgcg atacttggcg agaatggaat cagagtagtc
      361 taggccgaag tcacggaatg tggtctcgat ggcatgcctc tcagccctgt cgtaatcaaa
      421 gagagtgccg tctgcatcca gaagtatgag ttcgtagtcc attgtccatc ctccacaaca
      481 tggtcagtca gcattatacc gcatactgcc ctgctgatgt actggggaac acgcctttca
      541 ttcatgaccg ccgagtagcg ctgactgggg cctagttcgc tgaagtcacc ggccggagga
      601 gatcacggcg caatagcgaa atcccaaggg ccggtcgaag cttgactgcc ggagccgacc
      661 aacggggcgg cctatgttcg agcgaagagg tcggccaacc tcggcatcaa acaatcagag
      721 gtgggacatg ttcaggatcg gggagttctc caagctgact caggtctccg ttcgtatgct
      781 gcggcactac gatgaggttg ggttgctcag gcccgcccag atcgatgagg cgacaggcta
      841 tcggctctat tccgttcagc agattccagc agtccagaag atcgtcctgc tgcgcaacgt
      901 gggcttcggt attgccgaga tcgccgcggc gctggcagac tgggaccggc aatccatcac
      961 gcagcgcctg gtgaacaagg cgcgtgagat cgaagaagcc attcgtctgg aacgtgaaca
     1021 tgccgcaaga atagccgccg ccatcgacga tctacagacg ggcagaattg cggtccacta
     1081 caatgtgacg atcagaagca tacctggtta ccaaatcctg tcgctgcgaa ggacgatccc
     1141 caactatcac tgcgaggcag tcctgtggga cgagttgtgc gccttcgtca gcagagaaag
     1201 ggttgaactg catccgcgcg gcggcgccat cgcgttcttt cacgacgatg agcacaggga
     1261 ctccgacgtg gatgttgagg tcggcgtgat cgtcagcaag cctggggaca acaaggatgg
     1321 attcatctat cgcgaaacgg agaaggtgga caccatggcc tgtatcatgg tgtacggccc
     1381 ttttgagaat atcggcccat cgtatcagtc gtttgcctac tggttggaaa cacaccagca
     1441 ctacgccatg catgggccgg tccggcagat ctgtcacagg gggccgttca acgctcagaa
     1501 ttcggacgac tacctcaccg aggtacaagt gcctgtcaca aagcgactgc gagacgccgt
     1561 tccataggcg agctactcct gccgttgact ctcacattgt gtcagggttt atcatgagtc
     1621 cagaaacgcg aggaggtcat gatatggaac agctatacgt gaaacccatc ggacgaatcg
     1681 tgatcaacga aaacggcatg ttcgttaagg ttgaaccgca gtataccccg gcgctcaagg
     1741 agctggacgg tttcagccac ttgaacatct tctggtggtt cagcaacctc gataacgacg
     1801 tgtcacgcag cgtcctcgaa gtggatcgcc cgtacagaac gtctccagcc gttctgggta
     1861 cattcgcaac ccgttcccca caaaggccga acccaattgc cttgacagtt gcgcagatta
     1921 tccacatcga tcacaagaac ggcatgattc aggtcggcta tacagacgca gacaatggta
     1981 gcccggtgat cgatctgaag ccctataccc caagcctcga ccgcgtcgaa aacccctcag
     2041 tcccggaatg gtgccgccac tggccgaaaa gcttggagaa gtcggcagac ttcgactggg
     2101 atagcgagat gaactagcac ctgctcatcg ccgtggcgcc cttcgccgac cgccgttcgc
     2161 aaggtgcccg atgctactcc accagagact ccccacgtac cgttcgcttg tggtatccaa
     2221 ctccctgttg cattgcacga acgcgggcca ctccatctcc cgggagcgag actcccgggg
     2281 gtggccctac ttgagccggc ggccatgcga ctgcgatgcg cttgccacgc gaccgcgatc
     2341 gggtggacac cgcgaacgtc cgccatctac cgtccatttc ctccaccgta gatgttgccg
     2401 ctgggatcat ggcgttccac gtgttgcctc tcggtattgc gcaggtcatc ctttgtagca
     2461 gatgccttgg cctcgctgta ctctacccac tgcttctcag gatcgagctt ccccgagcgc
     2521 acatgttgct gttgcctgac ccgcagattc tcagtctgcc cgacgtattc cacctcgccc
     2581 gtcctcttgt taacgtgtct gtatacgccc ggcgaatccg gaatggtgca tcccgaacct
     2641 ttggtctgat cggtcttctt catacgaccc ctgggaatcg ccgacttgtc gccgcaagac
     2701 atgcgtagca cctcccaatt ggcgaataag ctgcagcgta cgcggacctc gccagtgatc
     2761 atctcctgcg cgtgctgctt ctcggcatcc agtggagacc tgcttctggg cgcatcggca
     2821 agtgccttca gcttggctga gccgaagcta accgaagtgc acgcaccgcc tacgacgcca
     2881 tggggcagga caaccaatat cactatcatt agccgttgat ctgttatgtc ctccccaatc
     2941 tcggtggaca tgagtcttca tcgttgtacc tcgatggcct gggtcacgct cccggcagag
     3001 gtgcagcaga ttccaaacgc gtagcgatac atgcaaggct ctgaccactg ccagcttgaa
     3061 ggaagatgca
//
LOCUS       DAOTAC010000291         3060 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_25145_length_3060_cov_5.643594, whole genome shotgun sequence.
ACCESSION   DAOTAC010000291 DAOTAC010000000
VERSION     DAOTAC010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25145_length_3060_cov_5.643594"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="PLM74_10530"
     CDS             <1..575
                     /locus_tag="PLM74_10530"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA-guanine transglycosylase"
                     /protein_id="HON43544.1"
                     /translation="VVQGGDDRELRRVCADQLAEMGFDGYGYGGWPIADDGRLVDGVG
                     MVAELTPDDVPRHALGIGKPENVVRAAAMGYDTFDAVIPTRDARHKRLYVACDGFASL
                     TWKAEDFYENLYIHDARFARDRRPVEQSCDCLCCRNYSRAYLRHLFDIGDSLALRLAT
                     IHNLRFYTRLMSRIREMLEEDRDGQEGGRG"
     gene            550..1377
                     /locus_tag="PLM74_10535"
     CDS             550..1377
                     /locus_tag="PLM74_10535"
                     /inference="COORDINATES: protein motif:HMM:NF018758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43545.1"
                     /translation="MGKKVGAADPVYEIVASLASSRKYAGLCPDTLHRTARWAWERYP
                     DLPAATKAAKRKLHQVFASFIREDDILKAQRLVETLPLCPDQDETRSVCRRIMELHSS
                     TRERMHNMEQLYPALLAAIGGPPRRIADLACGLHPFALPWMGLPPDTVYMASDIDWRI
                     VDSLNRFFDRLDISASAECRDLLADQSRVSADLVFLMKALPGLEQQEKGGAARVLRRL
                     DAEWVVVSTPTASLSGREKRMYENYDAMVSHVVEQVGWRALEMRSGNETFYVLRAES"
     gene            complement(1468..>3060)
                     /gene="groL"
                     /locus_tag="PLM74_10540"
     CDS             complement(1468..>3060)
                     /gene="groL"
                     /locus_tag="PLM74_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392083.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HON43546.1"
                     /translation="DARTALKNGVDAVASAVKVTLGPKGRNVVLERKFGSPVITKDGV
                     TVAKEIELEDPYENMGAQLCREVAAKTNDVAGDGTTTATVLAQTIVSEGLRNVTAGAN
                     PMFLKRGIDKAVSLVVDELHKVAIPVVGKEDIAHVASIAGNEESIGNWIAEAMDKVGK
                     DGVITVEESKGIDTSVEQVEGMEFDKGYISPYFVTNVESMEAEIEDPFILIYEKKITN
                     VQDLLPLLEKVARAGKPLVIIAEDIEGEALATLVVNKLRGILNVAAVKAPGFGDRRKA
                     MLEDISILTGGQFISEETGAKLEKVDLSQLGRAKTVKITKEKTTIVEGRGDKSKIEGR
                     INQIRKQIEDSDSDYDREKLQERLAKLAGGVAIIKVGASTETELKEKKHRIEDALSAT
                     RAAVEEGIVAGGGATYINIIPLLDSIEAEGDEKTGVTIIKRALEDPLRTIANNAGLEG
                     SVVVERVKAENKKGFGLNALTGEFCDLVKCGIVDPVKVTRSALQNAASIASMLLTTEA
                     IIADIPKKETPPPYPPGGGMDY"
BASE COUNT          560 a          919 c          918 g          663 t
ORIGIN      
        1 ccgtggttca ggggggcgac gaccgagagc tcaggcgtgt ctgcgcagat cagctggcgg
       61 agatggggtt cgacggctat gggtatgggg gatggcctat tgccgacgat ggacggctcg
      121 ttgacggagt gggcatggtt gccgagctta cgccggatga cgtccccagg catgccctgg
      181 gcataggcaa gccggagaac gtggtgcggg cggccgcgat gggctatgac acatttgacg
      241 ctgtgattcc cactcgcgat gcgcgccaca agcggctgta cgtggcatgc gatggttttg
      301 cttcgctcac ctggaaggcc gaggacttct atgagaacct gtacatccat gacgccaggt
      361 tcgcccgcga ccgccggccg gttgagcagt cgtgtgactg cctatgctgt cggaactact
      421 cccgtgctta cctgcgtcat ctgttcgata tcggagactc gctggcgctg cgactggcca
      481 ccatacataa cctgcgtttc tacacacgtc tcatgtcacg tatccgcgag atgctggagg
      541 aggatcgaga tgggcaagaa ggtgggcgcg gctgatccgg tatacgagat tgtcgcatcg
      601 ctagcgtcct ccaggaaata cgcaggtctt tgccccgaca cccttcaccg aacagcccgc
      661 tgggcatggg agcggtatcc cgatctgccc gcggccacga aagctgccaa aaggaagctg
      721 catcaggtat tcgcatcgtt catccgcgaa gacgacattc tgaaagcgca gcgcctagtg
      781 gaaacccttc ctttgtgccc ggatcaggac gagacgcggt cggtatgccg cagaataatg
      841 gaactacact cctctacacg cgagcgcatg cacaacatgg agcagctgta cccagccctg
      901 cttgccgcga taggcggacc gccgcgccgc attgccgacc tcgcatgcgg cctgcacccg
      961 tttgcgcttc catggatggg gctcccgcct gacacagtgt acatggcttc ggatattgac
     1021 tggcggattg tggactcgct gaaccgattc ttcgaccgtc tagacatctc agccagcgcc
     1081 gaatgtcgtg acctgcttgc agaccaatcg cgcgtcagcg ccgatctggt cttcttgatg
     1141 aaggccctgc ccgggctgga gcaacaggag aagggcggcg cagcgagggt tctccggcga
     1201 ctggacgcgg aatgggtggt tgtgtcaacc ccaacagcta gcctgtcggg gcgtgagaag
     1261 cggatgtacg agaattatga cgccatggta tcccacgtcg tcgagcaagt tggctggcgg
     1321 gcgctggaga tgcgctctgg caacgaaacc ttctatgtac tgagagccga atcctgacgc
     1381 agtgggcaga caggcctccg cccagcgttg ccaggcgaag gcctgctatc aatcacgcgc
     1441 agcccatggg ccgacacgtg ttcccgacta gtagtccatg ccgccgcccg gaggatatgg
     1501 cggaggagtc tccttcttcg ggatgtcggc gataatcgcc tcggtcgtaa gcaacatcga
     1561 cgcgatggac gcagcgtttt gcagagcaga gcgggtgacc tttacagggt ccacaatgcc
     1621 gcacttgacc aggtcgcaga actcgcccgt cagggcattg agcccgaaac ccttcttgtt
     1681 ttcggctttg accctctcga ccactaccga cccctcgagc ccggcgttgt tggcgatggt
     1741 ccgaagcgga tcctcgagag cacgcttgat gatggtcacc ccggtcttct cgtcgccctc
     1801 ggcctcgatc gaatctagca acgggatgat gttgatgtaa gtcgcgccgc caccggcaac
     1861 gatgccttcc tcaactgcgg ccctggtagc ggagagcgca tcctcgatcc tgtgcttctt
     1921 ctccttgagc tccgtctcgg tagacgcgcc gaccttgatg atggctacgc cgcctgcgag
     1981 cttagccagg cgctcctgga gcttctcgcg gtcgtagtcg gaatcggaat cctcgatctg
     2041 cttgcggatc tggttgatcc ggccttcgat cttcgacttg tcgccgcgac cttcgacgat
     2101 agtcgtcttc tccttggtga tcttgacggt cttggcccga cctagctggc tgaggtcgac
     2161 cttctcgagc ttggcaccgg tctcttccga gatgaactgc ccgccggtga gaatcgaaat
     2221 atcctcgagc atggccttgc gcctgtcgcc gaagccaggg gccttaacgg cggccacgtt
     2281 caggatgccg cggagtttgt tcacaaccag agtggcgagg gcttcgcctt caatgtcctc
     2341 agcgatgatc acgaggggtt tacccgcacg ggcaaccttc tcgagaagcg gcaggagatc
     2401 ctgaacgttc gtgatcttct tctcatagat gaggatgaag gggtcctcga tttcggcctc
     2461 catggattcg acgttggtca cgaagtaggg ggagatgtat cccttatcaa actccatgcc
     2521 ttcgacctgc tcaacgctgg tatcgatccc cttggattcc tcgacggtga tgacgccgtc
     2581 tttgccgacc ttgtccatgg cctctgcgat ccagttgcct atggactcct cgttgccggc
     2641 tatggaagcc acgtgagcaa tgtcttcctt gcccaccacg ggtatggcga ccttatgtag
     2701 ctcatctacg acgagcgaga cggccttgtc gattccgcgc tttaggaaca tgggattcgc
     2761 gccggcggtg acattgcgta gaccctcgct gacgattgtc tgagccaaca ccgtagctgt
     2821 ggtggtgcca tcgcccgcga catcgttagt cttggcggcg acttccctgc acagctgggc
     2881 acccatgttc tcgtaagggt cctcgagttc gatctccttg gcgacggtga cgccgtcttt
     2941 ggtgataacg ggtgaaccaa acttcctctc caacaccacg ttccggccct taggcccgag
     3001 cgtgaccttg actgccgacg cgactgcatc tactccgttt ttcagagcgg tgcgcgcatc
//
LOCUS       DAOTAC010000292         3040 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_25346_length_3040_cov_4.542714, whole genome shotgun sequence.
ACCESSION   DAOTAC010000292 DAOTAC010000000
VERSION     DAOTAC010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25346_length_3040_cov_4.542714"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="PLM74_10545"
     CDS             <1..403
                     /locus_tag="PLM74_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43547.1"
                     /translation="LELGYRQFSAIVSEIPAFFEYIDFRGPPDGIQIAKIGGYPVGPG
                     MASGTEGDRISHNMAVYGNTAQSMEYASAEPPHTRPPLTPRAPFAAPIRRLETPAQMH
                     VLKNCAYRQKYRQKLTACRLGYPRGYILELS"
     gene            806..1789
                     /locus_tag="PLM74_10550"
     CDS             806..1789
                     /locus_tag="PLM74_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HON43548.1"
                     /translation="MIAVTGGTGHIGNVLVRELVARGEQVRVLALPTEDTRPLDGLSI
                     QIVPGDVLDPHSLEAAFSGADIVYHVAGLVSIFPWQEQSVDRVNVQGTINVLNACEKA
                     GVRRLVYTSSIHSLVEPPHGAVIDESCGFDPERSRGGYDKSKARASIEVLRAVERGLD
                     AVIVCPTGVIGPFDFRVSIMGRVLLDLARDSFWAIPDGGYNFVDVRDVATGHILAAEK
                     GKPGEVYILAGECISVADLARMVDELRGRRPRSRLRLPAWLTRVIAHVSTAICLAFKV
                     RSRITKYSVETLLSNCNISNAKARRELGYAPRSLRDSVRDSIRWFADNGVA"
     gene            complement(1862..>3040)
                     /locus_tag="PLM74_10555"
     CDS             complement(1862..>3040)
                     /locus_tag="PLM74_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43549.1"
                     /translation="TYVEGDKFLAFGHPVLGNGTTDLAASSALVHGVIKSDSTPFKVL
                     SSTGWVGAFTQDRLSGVAGRLGRQAGLIPVSVTVVDKETGHERSISAQVAPEESLVAD
                     IFGSSALAAFDGTLDRVGGGTATVELRVELAGRQPYERVDTFWSNSDVAGASLSNAFD
                     TVDLIANNAAERATIERIVLKAQVGADRRTAMIEKARVLQKTARPGDQVEVEVTLRVF
                     RGPRVTRVLSLVIPDDIGSGTVSLMVRGGSVSVEDELDGWTTPVQELLYMDVDDMLAD
                     LSSRPLGNDIVLELEPYLLDEPEIDMTVPVGSELHRDGVSGRESDDKAKGKDKGKDKE
                     TQPGAADLQADADELFSKPVSVRVPTDWVVQGVKWLTIEVENEVDTEPAIEELYGPDM
                     "
BASE COUNT          589 a          940 c          873 g          638 t
ORIGIN      
        1 gctggagctg ggatatcgtc agttttcggc aatagtttcg gagataccgg cattttttga
       61 gtacatcgat ttcagagggc cgccggacgg catccagatc gcgaaaatcg gggggtatcc
      121 ggtcggtccg ggtatggcat cgggcacgga aggcgacagg atatcccaca acatggctgt
      181 atatggcaat actgcgcaat caatggaata cgcttccgcc gagccaccac atactaggcc
      241 gccgctgacg ccaagagcac ctttcgcagc accgatccgc cggctggaga ccccggccca
      301 aatgcatgta ctcaaaaatt gcgcctatcg gcaaaagtat cggcaaaaac tgacggcatg
      361 ccggcttgga tacccccggg ggtatatcct tgagctgtcg tgatcgcctt tctgccctcg
      421 gatctatccc tcggatctat cgctcggatc cattgccgga tctatcccgg gtccccttct
      481 gccccagccc ctctcactgt gatcctggtg aggtcgcttg ctgagggaca gacccggaag
      541 gggagtcgga tgggttgagt ggggcgccaa acgccgaatc ggcgcggtgc cggaactggc
      601 gcgaccccgg aactggcacg acccgcggct ccgatagcac caatccttac atgcacccct
      661 gacaggtcgg ccaacaagtc ggatagccag ttgacagcac gaaacctcag ctgtacactg
      721 tgaatgagca ggtgactgac tgttcagtca gtgggccggt tcggctcaac tggcgaatga
      781 ccagctctcc gaggcggtga gtgcattgat agcagtaact ggaggaactg ggcacattgg
      841 aaacgtgctc gtgcgggagt tggtagcccg cggtgagcag gtacgagtcc ttgcgcttcc
      901 aactgaagac acacgcccgc ttgatggcct gtcaatccag atcgtcccgg gagacgtgct
      961 ggacccccat tcgctggaag ccgcgtttag tggtgccgat attgtctacc acgtggctgg
     1021 actggtgagc atcttcccct ggcaggagca gtcggtggac cgcgtgaacg tgcagggcac
     1081 cattaacgtt ctgaatgcct gcgagaaggc gggcgtgaga aggttggtct acactagctc
     1141 gatccactct ctcgttgaac ccccgcacgg cgctgtcatc gacgaatcat gcggtttcga
     1201 tcctgagcgt tcgcgcggtg ggtatgataa gtcaaaagct agggcatcga ttgaggtcct
     1261 ccgggcggtg gagcgtggac tggacgcagt gatagtctgc ccgaccggag tcatcggccc
     1321 cttcgatttc cgcgtgtcga tcatggggcg tgtgctgctt gatctggcgc gcgattcgtt
     1381 ctgggcgatt ccggacgggg gctacaattt cgtagatgta agggatgtcg cgaccggaca
     1441 catcctagca gccgagaaag gcaagcccgg tgaagtgtat attctagccg gcgagtgcat
     1501 tagcgttgcc gacctggcgc ggatggtgga cgagcttcgc gggcgccggc cgcgctcccg
     1561 gctgcgtctg cctgcctggc tcacgcgggt catcgcccat gtgtcgacag ccatctgcct
     1621 ggcgtttaaa gtgagaagcc ggattaccaa atattctgtg gagacgctac ttagcaactg
     1681 caacatcagc aacgcgaaag cccggcgaga actgggctat gccccgaggt cgcttcgcga
     1741 ctcggtgaga gactctatcc gatggtttgc cgacaacggg gtggcctagg cggtccgctc
     1801 cacggccacc ccctccacat tcccagcttg ctggagtctc ccgactcaag ctctcacgct
     1861 ctcacatatc gggtccatac agctcttcga tcgccggctc agtatcgacc tcgttttcaa
     1921 cctcaatcgt gagccacttt accccctgaa ctacccaatc tgtcggaacc cgcaccgaca
     1981 ctggcttgga gaacaactca tctgcgtctg cctgcagatc ggcagcgccc ggctgggtct
     2041 ccttgtcctt gcctttgtcc ttgcctttcg ccttgtcatc ggattcgcgc ccggaaacgc
     2101 cgtcccggtg caattccgag cccaccggca cggtcatgtc aatctcgggt tcgtccaaca
     2161 aatagggttc gagttcgagc acgatgtcat tgccgagagg acgtgaactc agatcggcca
     2221 gcatgtcatc gacgtccatg tagagcaatt cctgcaccgg cgtggtccag ccgtccagtt
     2281 catcctcaac gctgacgctg ccacccctga ccatgaggga gactgttccg ctccctatgt
     2341 cgtccggtat caccaaagac aacacccggg tcaccctcgg ccctcggaac acgcgcagcg
     2401 taacctcgac ttccacttgg tctcccgggc gcgcggtctt ctggagcact ctggctttct
     2461 caatcatcgc agtgcggcga tccgctccta cctgggcctt aaggactatc cgttcaatgg
     2521 tggcacgttc ggccgcgttg ttcgcaatca ggtccactgt atcgaaggca tttgacagac
     2581 tggcgcctgc tacatcggag ttgctccaga atgtgtccac gcgctcatac ggctgccttc
     2641 ccgcaagctc cacccgtagt tcgaccgttg cggtgcctcc gcccactctg tcaagggttc
     2701 catcaaacgc agcaagagcc gatgagccga aaatgtcggc tacgagggac tcctccggcg
     2761 caacctgcgc agatatgctt cgttcatgcc cggtctcctt atcgacgacc gtcactgata
     2821 ctggaatcag gcccgcctgc cgccctagcc tgcccgcgac tccgctgagg cggtcttgag
     2881 tgaatgcacc cacccagccc gtggagctga gcaccttgaa aggcgtgctg tcagacttga
     2941 tcactccgtg taccagcgca ctgcttgcag ccaagtcggt cgtgccgttt cccagcacgg
     3001 gatggccaaa ggccaggaac ttgtcgcctt ccacataggt
//
LOCUS       DAOTAC010000293         3029 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_25468_length_3029_cov_4.724950, whole genome shotgun sequence.
ACCESSION   DAOTAC010000293 DAOTAC010000000
VERSION     DAOTAC010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25468_length_3029_cov_4.724950"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..2797
                     /gene="ppdK"
                     /locus_tag="PLM74_10560"
     CDS             98..2797
                     /gene="ppdK"
                     /locus_tag="PLM74_10560"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HON43550.1"
                     /translation="MAEKEIPKYVYYFGGGRAEGSGNMKNLLGGKGAGLAEMVNLGIP
                     VPPGFTITTEVCTAYYASSNQWPEGLAEQIDANLARLEKDMGAVFADPGNPLLLSVRS
                     GARVSMPGMMDTILNLGLNDVTAAGLIKKTNNPRFVYDSYRRFVHMYSDVVLGVPHEK
                     FEETIAAKKRARGVKLDMELSADDLAQLVAEYKAITREHTGHDFPEDPKEQLRGAVDA
                     VFGSWNNQRAITYRKINNIPGDWGTAVNVQTMVFGNMGETSGTGVAFTRDPATGENVF
                     YGEYLMNAQGEDVVAGIRTPQPISTLKESMPEIYDELEQIYHKLEAHYREMQDIEFTI
                     QDGRLFMLQTRTGKRTGTAAVRIAVEMVQEGLIDEKTAVLRVPPANLDQLLHPMIDTK
                     AKVRAFAKGLPASPGAAVGRIVFTADEAEAWAERGEKVILVRTETSPEDIGGMHAAVG
                     ILTSRGGMTSHAAVVARGMGTCCVAGCGAILINEREKKMVCDDLMLGEGDWITLNGST
                     GEVIEGKAPLVKPELTGSFGKLMEWADKFRALKIRTNADTPQDAKQARMFGAEGIGLC
                     RTEHMFFAEDRIRAMREMILSDTENQRRGALAKLLPFQKGDFVGILREMAGLPVTIRL
                     LDPPLHEFLPTMEQTKQISEIAAEMGISEAKVRERIQSLHELNPMLGFRGCRLGVVFS
                     EIYEMQVKAIFEAACELAREGVEVMPEVMIPLVGTTGEMEMIREYAVRIAEETIREYG
                     VKLHYLVGTMIEVPRAALIADRIARTAEFFSFGTNDMTQMAFGYSRDDAGKFLAQYMD
                     RNVLSADPFQSLDQEGVGQLVEMGVQRGRSTRPNLKVGICGEHGGDPDSIGFFHRVGL
                     DYVSCSPYRVPIARLAAAQAELKNPRKAQEEACGGGCCRKTCR"
BASE COUNT          665 a          817 c          987 g          560 t
ORIGIN      
        1 agggcgattg tgaatttcgc ttgaacggga gctctcagta gagtagaatc gtaggcgact
       61 tggtaaacag agagcagcaa tggacaggag gattgtcatg gcagagaaag agataccgaa
      121 atatgtgtat tatttcggag gcggaagggc ggaaggctcg ggcaacatga agaaccttct
      181 gggcggcaaa ggcgccgggc tggctgaaat ggtgaatctg gggattccgg tacccccggg
      241 cttcaccata actaccgagg tatgcacagc ctactacgcc agcagtaacc agtggcccga
      301 gggtcttgca gagcagatcg acgcgaacct tgcgcgactg gagaaagata tgggcgcagt
      361 gttcgccgat cctgggaacc cgcttctgtt gtcggtgagg tcaggcgctc gcgtatcgat
      421 gcccggcatg atggacacca tcctcaatct gggtctgaat gacgtgacag cggcagggct
      481 aatcaagaag accaacaacc ctcgtttcgt gtatgacagc tacaggcggt tcgtgcacat
      541 gtactccgac gtggtgttgg gcgttcctca cgagaagttc gaggagacca tcgccgcgaa
      601 gaagcgggcc cgcggcgtga agctcgatat ggagctgtcc gccgacgacc ttgcccagct
      661 agtcgccgag tacaaggcga tcacccggga gcacacaggg cacgatttcc ctgaggatcc
      721 caaagagcaa ctacggggcg ccgtggatgc ggtgttcggg tcgtggaata accaacgggc
      781 gattacatac cggaagatca acaacatacc cggcgactgg ggcaccgcgg tcaacgtgca
      841 gacaatggtc ttcggcaata tgggcgagac gtccggaact ggcgtggcgt tcacgcgcga
      901 tcccgccacc ggcgagaatg tgttctacgg agagtacctc atgaacgcac agggcgagga
      961 cgtcgtagcc ggcattcgca cgccccagcc tatcagcact ctgaaggaat cgatgcccga
     1021 gatctatgac gagctcgagc agatatatca caagcttgag gcgcactacc gcgagatgca
     1081 ggacatcgag ttcacgatac aggatggccg tctgttcatg ctgcagacgc gtacaggcaa
     1141 gaggaccggc acggccgctg tacgcatagc cgttgagatg gttcaggagg gcctaattga
     1201 cgagaagact gctgtgcttc gtgtgccccc cgctaacctc gatcagctgc ttcacccaat
     1261 gatcgacact aaagccaagg tccgggcgtt tgccaagggc ctgccggcat cgcccggcgc
     1321 tgccgttggg cgcatagtat tcaccgccga cgaagctgag gcatgggctg agcgcggcga
     1381 gaaagtgatc ctggtcagaa cagagacttc gcctgaagat atcggtggaa tgcacgctgc
     1441 ggtgggcatc ctcacatctc gtggcggcat gaccagccat gcggcagttg tggcccgcgg
     1501 catgggcacc tgctgcgtcg ccgggtgcgg agccattctc atcaatgaga gagagaagaa
     1561 gatggtctgc gacgacctta tgctgggaga gggcgattgg atcaccctga atggttccac
     1621 cggtgaggta atcgaaggga aagcgcctct ggtcaagccc gaactgacag gcagctttgg
     1681 gaagctcatg gagtgggccg ataagttccg tgcgctgaag atccgcacca acgcggacac
     1741 gccgcaagat gccaagcaag cccggatgtt cggcgccgag ggtattggcc tatgtaggac
     1801 cgagcacatg ttctttgcgg aagaccgaat ccgcgccatg cgcgagatga tactttccga
     1861 taccgaaaac cagaggcgcg gagcgttggc caaactgctg cctttccaga agggcgactt
     1921 cgtcggaatc ctcagggaga tggcagggtt gcctgttacc atcaggctcc tggatccgcc
     1981 gctccacgag ttccttccga ccatggagca gaccaagcag atatcggaga tcgctgccga
     2041 aatgggcatt tccgaggcga aggtgcgcga acgcatccag tccttgcacg aactcaaccc
     2101 catgctgggg ttcaggggct gccgccttgg ggtagtattc tctgagatct acgagatgca
     2161 ggtgaaggcg atcttcgagg ccgcctgcga gcttgctcgc gagggcgtag aggtgatgcc
     2221 tgaggtgatg attccccttg tcggcaccac cggcgagatg gagatgatcc gcgagtacgc
     2281 agtgagaatc gccgaggaga cgatccggga atacggcgtc aaactgcatt acctagtcgg
     2341 aacgatgatc gaggttccgc gtgctgcgct catcgccgac agaatcgccc ggaccgctga
     2401 gttcttctcg ttcgggacca acgacatgac ccaaatggcc ttcggctata gccgcgacga
     2461 cgcgggcaag ttcctcgctc agtacatgga caggaacgtg ttgagcgcgg atcctttcca
     2521 atctcttgat caggaaggcg tgggccaact cgtggagatg ggcgtgcagc gcggacgcag
     2581 cactcggccg aacctcaagg tcggcatctg cggcgagcac ggcggggatc cggattccat
     2641 cggtttcttc caccgcgtgg gtctggatta cgtgagctgc tctccatatc gtgtgcccat
     2701 cgctaggctg gccgccgccc aggcggagct gaaaaacccc agaaaggccc aggaagaggc
     2761 ttgcggcggg ggctgttgca ggaaaacttg tcgctagagg tgaaatgagt atacagggtc
     2821 agccagtggc ccacgcggca tgttgctgcg tgggccattt gccccattga tacgaggagg
     2881 ggtgccctgt gacggtgagg gagaggatcg agcgggagga ggctgcccgt ctttcggcgc
     2941 atgcggcgct cgctgccaat tcgcgtgggc gcgatatggg tgaggaggcc gatgagtttc
     3001 gcacgtgcta tcagcgagat cgcgatcgg
//
LOCUS       DAOTAC010000294         3014 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_25628_length_3014_cov_5.349104, whole genome shotgun sequence.
ACCESSION   DAOTAC010000294 DAOTAC010000000
VERSION     DAOTAC010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25628_length_3014_cov_5.349104"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..717)
                     /locus_tag="PLM74_10565"
     CDS             complement(139..717)
                     /locus_tag="PLM74_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43551.1"
                     /translation="MEECTYDEIGRKSLAAFSNLLDILSGTKSASISHDQASIERRLG
                     DQLASLAASLEVVRRDLAAFASRTDRVLLDLLKRMEAQEDLTSTMGSAADSLSLAGQA
                     QVMGAAGRQAGPGTGRAGGSAKTGTARGKYERVPKEQTKKKILEAARELAASGRKVTL
                     AECARLAGVAYYKAVYACKDSDELAGLMVSSE"
     gene            996..2405
                     /locus_tag="PLM74_10570"
     CDS             996..2405
                     /locus_tag="PLM74_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009850192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HON43552.1"
                     /translation="MEKTDNLPHVSLRDRVDALLKALPIAVAVMNRDGVVRYGWNSAA
                     ENLFGRPRSEAVGKVIPLLDGGCSVDFADLLRRAATGEVISGTEFHYERRDGSTVYAS
                     VSLAPAANVVGNRQGTRAIVGLFEEITQRKQAEALLRESEERFRSVFEDGQLGMAIVD
                     LDTRIVQANRRMAEVFGCAPRELAGSMVKDLSHPDDVADVAARIERLAAGNIDYFDAE
                     NRYIRKDGSTLIGRVVGTLIGSAGETRSRHIIAMLEDVTARRQTQEELSSTMAKLRQS
                     MRQGIRAMAHIVEVRDPYTAGHQERVACLARAISQTLGLPEDEVAGIYYASLVHDIGK
                     ISIPGEILSKPGRLSSAEFELIRVHPQAGYDIVRSIDFPWPVNDIVLQHHERLDGSGY
                     PQGLTGDQISYGAVIVAVADVVEAMSSHRPYRPALGIEAALDEIRKNRGRLYDPGVVD
                     ACESIVLTQGIDALVSSTP"
     gene            complement(2395..>3014)
                     /locus_tag="PLM74_10575"
     CDS             complement(2395..>3014)
                     /locus_tag="PLM74_10575"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HON43553.1"
                     /translation="GDIADQTNLLALNAAIEAARAGEQGRGFAVVADEVRKLAERSLA
                     ETKAISKLIEVTVSDTSRVSTAIETSGKLVEESMPLVEASILSLKRIGEHASDNLTLV
                     DSAVKLRQSVMEAAGKVRESMVEAVAVADENAAAAGQMAAGIAEVQKSIESVAAVSDE
                     NAAAAQEVSASAEEVGASIEEMASASHSLDEMAGRLQELAAQFKA"
BASE COUNT          563 a          892 c          919 g          640 t
ORIGIN      
        1 aacgtgtggg tgcgccgaca ggttgtcagc cctacctatc ggcacattgg caggttctgc
       61 aggtctgccg ggagcaggcg ggcattcggc caccggggcc gctcaccaac ttctcccggc
      121 cgaccttgcg aatagccgct actctgagct caccataagt cccgcaagct cgtctgagtc
      181 tttgcaggca tagactgctt tgtagtatgc cactcctgcc agccgggcgc attctgcgag
      241 tgtcaccttc cgtccgcttg cagcaagctc ccgcgctgct tcgagaatct tcttcttcgt
      301 ctgctccttg ggcactctct cgtacttgcc ccttgcagtg cccgtcttag cagacccccc
      361 tgcccgtcct gtgcctggcc cggcctggcg gcctgctgcc cccatgacct gagcctggcc
      421 tgcgagcgat agggagtctg ccgcactgcc catagtcgac gtcaagtcct cctgcgcctc
      481 catacgtttt agtagatcga gaagaaccct atcagtcctg ctcgcaaagg ccgccaaatc
      541 gcgccggact acctcgagcg atgctgccag cgatgccagc tgatcgccga ggcgcctttc
      601 aatcgacgct tggtcatggc tgatggatgc ggatttagtc ccggacagga tgtccagcag
      661 attggagaag gcggcaagcg actttctgcc gatctcgtcg tatgtgcatt cttccatagt
      721 cagaacctcc tcatacaatg aagggatctc ttcacgggca cgtctggccg ccgcttcttg
      781 aacaatggcg gcgtaatagc gctgtttgcc gcgggagcac aattagatta cacctcctgc
      841 cacattatta ttaccaatcc tgccgaacat gtgcgtcgcc gtacgttgcc ggcgcgatat
      901 ctcagcgggg cggtccgggg agggggagga agaacggcgg cgcgactcga atactggtca
      961 gtgcattgtg gcattggcat aggagtatcg agtcaatgga aaagacggat aatctgcccc
     1021 acgtcagtct cagggaccgc gtcgatgcgc ttctcaaggc acttcccatt gcagtggctg
     1081 ttatgaaccg cgatggggtt gtgaggtacg ggtggaactc ggcagcggag aaccttttcg
     1141 ggcgcccgag gagcgaggcg gtcggcaagg tcatccccct gttggacggg ggttgcagtg
     1201 tggacttcgc ggatcttctt cggcgcgccg cgacaggtga agtaatctcc gggactgaat
     1261 ttcactatga gcggcgggac gggtcaaccg tatacgctag tgtttcactg gcacctgcgg
     1321 cgaatgtcgt tggcaaccgc cagggaactc gcgctattgt cggcctgttt gaggagatta
     1381 cccagcgcaa gcaggctgag gccctcttgc gcgagagtga ggaacggttc aggagtgtgt
     1441 tcgaagacgg ccagctgggc atggccattg tcgatctgga tacccggatt gtgcaggcga
     1501 acaggcgaat ggccgaggtg ttcggctgtg cgcctcggga actggccggg tccatggtga
     1561 aagacttgag ccatcctgat gacgtagcgg atgtagctgc ccggatagag cggctcgctg
     1621 ccggcaacat cgactacttc gacgcagaga accgatacat ccgcaaagac ggttctactc
     1681 tcatcggaag agtcgtgggc actttgatcg gctcggcggg cgagacgcga tctcgacaca
     1741 ttatcgcgat gctcgaagat gtgaccgcgc gaaggcagac tcaagaagag ctttcaagca
     1801 ccatggccaa gctcaggcag agcatgaggc agggcatccg ggccatggcg cacatcgtgg
     1861 aggtacggga cccctacacc gcgggccatc aggagcgcgt tgcgtgcttg gcgcgggcga
     1921 tatctcagac gctggggctg cccgaagacg aggtggccgg gatttactat gcctcgcttg
     1981 tgcatgacat cggcaagatc agcattccgg gtgagatatt gagcaagccg ggcaggctga
     2041 gcagcgctga attcgagttg atccgagtgc atccgcaggc gggatacgac atagtgcggt
     2101 cgatcgattt tccgtggccc gtgaatgaca tagtcttgca gcatcatgaa cgcctggatg
     2161 gttccgggta tccccagggg cttacaggcg accagatctc ctacggcgct gtgattgtgg
     2221 ctgttgcaga cgtagtggag gcgatgtcgt cacaccgccc ataccgcccg gcactgggga
     2281 ttgaggcggc gctggatgag ataaggaaga accggggccg tctgtacgac cccggcgtag
     2341 tggatgcatg cgaaagcatc gtgttgacgc aaggtataga cgctctggtt tcgtctacgc
     2401 cttgaactgg gcggcgagct cctgaaggcg gccagccatc tcgtcaaggg agtgagatgc
     2461 cgacgccatc tcttcgatgg acgcgcccac ctcctcggct gaggcagaca cctcttgagc
     2521 agcagcggca ttctcgtccg acactgccgc cacgctttcg atggactttt ggacttcagc
     2581 gatgccggca gccatctgcc ctgccgcggc cgcgttttcg tcggctacgg ccactgcctc
     2641 gaccatcgac tcccgcactt tgcccgctgc ctccatgacg ctctgtcgca gcttgacagc
     2701 tgaatcgact agcgtcaggt tgtcggatgc gtgctcgcct atcctcttca gcgatagtat
     2761 agacgcctcc accagcggca tgctctcctc aaccagcttg cctgaggtct ctatggctgt
     2821 cgacaccctg ctggtgtcgg atactgtgac ttcgatcagc ttggatattg cctttgtctc
     2881 cgcaagcgac cgctctgcca gcttccgcac ttcatcagct accaccgcga atcctcgacc
     2941 ctgctccccg gcgcgggcgg cctcgattgc tgcgtttagg gccagaagat tagtctggtc
     3001 cgcgatgtcg ccga
//
LOCUS       DAOTAC010000295         3012 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_25654_length_3012_cov_4.820426, whole genome shotgun sequence.
ACCESSION   DAOTAC010000295 DAOTAC010000000
VERSION     DAOTAC010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25654_length_3012_cov_4.820426"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="PLM74_10580"
     CDS             <1..582
                     /locus_tag="PLM74_10580"
                     /inference="COORDINATES: protein motif:HMM:NF014926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HON43554.1"
                     /translation="THSLIPEGRLPEIPRIGMQTEIPGEFVTTTWYGRGPHENYWDRK
                     TSARVGVYSMPTVELAHPYCRPQETGNRCDVRWVAFTGPDGAGILAAGMPTIEFSAWP
                     CAMDDLESARHVHEVPRRGWFTVNLDYRQMGVGGDNSWGAKTHPEYVLYPMPYTYSFR
                     IRPYVPIMGDPSTVAMLSLPGGWAVGCIPGNCV"
     gene            614..1402
                     /locus_tag="PLM74_10585"
     CDS             614..1402
                     /locus_tag="PLM74_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HON43555.1"
                     /translation="MGQATIETLLRLHGEVALVTGAASGIGRGVAHRLAQAGASVALL
                     DIDTAGGERAAREIGQSTGAPTLFVSCDVSSAQGCERAVRQAVERFGYIDVLVNNAGV
                     IRRKNVVDLLEAEWDLVVDVSLKGAYLMSKHVIPCMRPDRGGSIVNMGSGWGLKGGPD
                     AAAYCAAKGGIVNLTRAMAIDHGPAGIRVNCVCPGDTDTPLLRSEAVQLGADMDEFLR
                     EAAARPIGRVGTPDDIANAVLFFASPMSTWVTGATLVVDGGGLA"
     gene            1421..2836
                     /gene="gcvPA"
                     /locus_tag="PLM74_10590"
     CDS             1421..2836
                     /gene="gcvPA"
                     /locus_tag="PLM74_10590"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HON43556.1"
                     /translation="MSDMIHPYIPNSVPEVKREMLKAIGASSTEELYTDIPEELRFRR
                     TMNLPHPMTAESDLVRHVMGILGRNQTCEENISFLGGGCARHYVPAICDEINGRSEFL
                     TAYAGEPYEDHGRFQALFEYQSLMAELLDVDVVNVPTYDWGQAASTALRMAARIVRRN
                     TVLVSDTVSPERLAVIMNYCTPALRIIPVAHSRVTGMMDLADLELKMSDETAAVYFEN
                     PSYLGLIETEGETIADIAHRRGALLVVGADPASLGVIVPPSHYGCDLVCGDIQALGVH
                     LHYGGGLGGFIGCRDEERFVMEYPFRLFGIARTCEQGEWGFGDVAYSRTSFAQREEGK
                     EFVGTAAALWGITAAVYLSLMGPEGMCKLGTTIMQKSQYAMARLSGIPGVKAPAFDAP
                     HFKEFVVDFNGTGRTVQQINEHLLAHGVFGGADLSRDLPNLGQSALYCVTELHTQEHL
                     DYLAEAISCAITGGECSGHSA"
     gene            2820..>3012
                     /locus_tag="PLM74_10595"
     CDS             2820..>3012
                     /locus_tag="PLM74_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009672277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase subunit 2"
                     /protein_id="HON43557.1"
                     /translation="MATVRKFHQARWNEPVIFELSRPGERGIMVPEAEPGIRERVGDG
                     TSRLPGKMIRQDMPALPEVA"
BASE COUNT          634 a          830 c          960 g          588 t
ORIGIN      
        1 acgcactcac tgatccccga aggacgcctg cccgagatac ctcgcatcgg aatgcagaca
       61 gagatacccg gggagttcgt gactacaacc tggtatggac gcggtccgca tgagaactac
      121 tgggatcgaa agacaagcgc ccgagttggc gtctactcga tgcccacagt cgaactagct
      181 catccgtatt gcaggccgca agagacaggc aataggtgcg acgtacgctg ggtagcgttc
      241 accgggcctg atggagcagg cattctggca gcaggcatgc ccacgattga gttctctgca
      301 tggccgtgcg ccatggacga cctcgaatcg gcgaggcacg tccatgaagt gccgcggcgt
      361 gggtggttca cggtgaatct ggactaccgt cagatggggg taggaggcga caacagctgg
      421 ggcgcaaaga ctcatcccga gtacgtactg tatcccatgc cctacaccta ctccttcaga
      481 atcaggccct acgtgcccat catgggtgat ccgtcgactg tggcgatgct tagcctgcca
      541 gggggttggg ccgtaggctg catcccgggc aactgtgtgt gaagactcaa ggctagacgg
      601 gaggcgatat cacatgggtc aagcgacgat tgagaccctt ctcagactac acggagaagt
      661 cgctctagtt accggggcag cctccggcat cggtcgtggc gtggcgcaca ggctggccca
      721 ggccggggct tccgtggcgc tgctcgacat agacacggcg ggcggcgagc gagcggcccg
      781 cgaaatcggg cagagcaccg gggcgccaac gctctttgta agctgtgacg tgtcctcggc
      841 tcagggctgc gagcgcgcgg ttaggcaagc agtggaaagg ttcggctaca ttgatgtctt
      901 ggtcaacaac gctggagtga ttcgacgcaa gaatgtagtt gatcttctcg aggctgagtg
      961 ggaccttgtg gtcgacgtga gcctgaaggg cgcctacctg atgtcgaagc acgtgatacc
     1021 ctgcatgcgc cccgacaggg gaggatccat agtgaacatg ggatctggtt ggggcctcaa
     1081 gggaggaccc gatgccgccg cctactgcgc tgctaagggc ggcatcgtga acctcacacg
     1141 ggctatggcc atcgatcacg gtccggctgg gatcagagtc aactgcgtgt gcccaggcga
     1201 caccgatacc ccgcttctca ggagcgaggc ggtgcaactt ggagcggaca tggacgaatt
     1261 cctgcgtgag gcggctgctc gccccatcgg gcgtgtggga acgcccgatg acatcgccaa
     1321 tgccgtgctg ttcttcgcat cgcccatgtc gacatgggtt accggcgcca cactcgtcgt
     1381 ggacggcgga ggactcgcct gactgaggga gggtctgatc atgagcgata tgatccatcc
     1441 atacataccc aattccgtgc ctgaagtgaa gcgcgagatg ctcaaagcga tcggcgcatc
     1501 cagcacggaa gagctctaca ccgatatccc ggaagaactc aggttccgcc ggacgatgaa
     1561 tcttcctcat cccatgaccg ccgagtccga tctggtgcgg catgtcatgg gcatactcgg
     1621 caggaaccag acatgtgaag agaacatcag cttcctcggc ggagggtgcg caagacacta
     1681 cgtgcctgcg atatgtgatg agataaacgg gcgctctgaa tttctcactg catatgcagg
     1741 cgagccatac gaagaccacg ggcgattcca ggcccttttc gagtaccaga gcctgatggc
     1801 tgagttgctc gatgtggatg tagtgaatgt gccgacctac gactgggggc aggccgcaag
     1861 cacggccctg cgtatggctg caaggatcgt cagacgcaac acagtcttgg tgtccgatac
     1921 tgtcagccca gagaggttgg cagtgatcat gaattactgc acacccgcgc tacggattat
     1981 ccccgtagcc cactcccgcg tcacggggat gatggatctt gcggaccttg agctgaagat
     2041 gtcggacgaa accgcggcag tatacttcga aaacccgtcg taccttggat tgatcgagac
     2101 tgagggcgaa actattgccg atatcgcgca taggcgcggt gcgctgctgg tcgtaggcgc
     2161 ggatcctgct tcgctgggag taatagttcc tccctcacac tacggatgcg atctggtctg
     2221 cggcgatatc caggcgctgg gcgtccacct acactacggt ggaggcctag gagggttcat
     2281 cggttgccgc gacgaggaac ggttcgtgat ggagtacccg ttccggttgt tcgggattgc
     2341 caggacgtgt gagcagggag aatggggctt tggagacgtt gcctatagcc gcacatcctt
     2401 tgcccagcgt gaggagggca aggagtttgt cggcaccgcc gccgctctct ggggtatcac
     2461 ggcagccgtg tacctgtcgt tgatggggcc tgaaggcatg tgcaagctgg gcaccacgat
     2521 aatgcagaag tcacaatacg cgatggcgcg tctgtctggg attcccggcg tgaaggcacc
     2581 ggccttcgat gcccctcatt tcaaggagtt cgtcgttgac ttcaatggca ccgggcgcac
     2641 agtgcaacag atcaacgagc atctcctggc acacggggtc ttcggaggag cagatctgtc
     2701 tcgcgattta ccgaatctag ggcaatctgc actctactgt gtaactgaac ttcacaccca
     2761 ggagcatctg gattatctgg cagaggcaat cagctgcgcc ataacgggag gcgagtgtag
     2821 tggccacagt gcgtaagttc catcaggcaa gatggaatga accggtcatc tttgagcttt
     2881 cccggccggg agaacgtggc ataatggtac ctgaggcgga acctgggatc cgggagcgcg
     2941 tgggcgatgg gacatctcgc cttccaggaa agatgatacg ccaagatatg ccggcgctcc
     3001 ctgaagtggc cc
//
LOCUS       DAOTAC010000296         3007 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_25702_length_3007_cov_4.736450, whole genome shotgun sequence.
ACCESSION   DAOTAC010000296 DAOTAC010000000
VERSION     DAOTAC010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25702_length_3007_cov_4.736450"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            418..1806
                     /locus_tag="PLM74_10600"
     CDS             418..1806
                     /locus_tag="PLM74_10600"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HON43558.1"
                     /translation="MNHTTFPPGFLWGAATSAYQIEGATCADGRGVSIWDTFCAVPGK
                     VFGGHSGEPACQHYFKYAEDVGIMRELGLDSYRFSLAWPRLMPEGRGTLNRKGLDFYR
                     RLIDLLLESEIRPMATLYHWDLPQTLQDKGGWANSDTPKRFADYCVTVFGELADVVPM
                     WITINEPAVTAVLGHVTGEMAPGHRDAHEGLLVAHHLLLAHGLAVRAFRDSSSRKRAA
                     GTDASQPVDGIGIALSINYQEPATDDELDIAAAKRVDGFWNRLYLDPLFRGHYSEDVL
                     AWLAQAGVLPDALARPRPEEMRTISQETDFLGINYYTRNRVAWDPADRLLNASAIAPR
                     LPVTAMGWEVYPEGLFKVLKWVSDDYTKIPLFIAENGAAFDDPLPADGSIIDDSARID
                     YLSSHIEAAHKACQRGVNLRGYYVWSLMDNFEWAQGFSKRFGLVHVDFPTQQRTYKCS
                     ALWYRDLIRSQR"
     gene            1993..2904
                     /locus_tag="PLM74_10605"
     CDS             1993..2904
                     /locus_tag="PLM74_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HON43559.1"
                     /translation="MKRHRQGLLLTLAMGMLLVIAVTATGCNGKAYSVSGRVTIIGTG
                     IGIPNATVACGVYTAFTDSSGYWGVSGLGSTTTVSVTKEGWKFEPASVRVYGDRTDVN
                     FQGSELDYRGLSSDSSQWYYDVGVTATGQPDLIGWIRQRVTRVERESRRTLFHMRYEI
                     GGDMFRGSLRDLAPWFPGAGAASEAYDYFIAREGATYYLLPDRDSTPIFMLNKPLYQG
                     ATYNDMVIELPFELTVQREEEVTVPAGTFSTWYCSRLATDDGMTYLARAWFAPYVGPV
                     KYMIEVTDTSGSGMYGCIVVALRSYNE"
BASE COUNT          648 a          838 c          873 g          648 t
ORIGIN      
        1 ggacatgtac agtcagcaaa aatgacaaca cgactaggac ggccatgatc gtggctgcgc
       61 ttcgtcttct cattgttcac cctccatatg tatcgtggtg ctattgcgcg tatctaggac
      121 tttcggtgct cttccatgat tctcctgcca ttgttcatct ctatgggcag tagggcttgc
      181 cggtcgcctc ggcccatccg tcggtgtacg cgttgccccg ccagggactg agggatcact
      241 tcgactaccg tactatttga agccacgaat cagatttcag gtatgtcatc catcttggtg
      301 tatgatagtc ttggcaacag tggaacgcat ttacgatgcg cctcgtcatg ccgcagggcg
      361 ccatgagggc cctgcactgg acgagggata gtgaacgaca ccactcggga gggacacatg
      421 aaccacacca catttccccc aggcttcctg tggggagcag cgacatctgc atatcagatt
      481 gaaggggcta cgtgcgcgga cggacgaggg gtgtcgatat gggacacgtt ctgtgctgtg
      541 cccggcaaag tcttcggcgg ccattcgggc gaaccggcct gccagcacta cttcaaatat
      601 gccgaggatg taggcataat gcgcgagctt ggcctggatt cctacaggtt ctcgctggcc
      661 tggccgcggt tgatgccgga gggacgcggc acgctgaacc gcaagggact tgacttctac
      721 aggcgtttga tcgatttgct cctggaaagc gagattcgcc ccatggccac cttgtatcac
      781 tgggacctgc cgcagactct tcaggacaag ggcggatggg caaactcgga cacccccaag
      841 cgttttgccg attactgcgt caccgtcttc ggcgagctag cagatgtggt tccgatgtgg
      901 atcaccatca acgagccggc agtcacagcg gtcctcggtc acgtcaccgg cgagatggcg
      961 cccggccatc gcgatgccca cgaaggcctt ttggtggccc atcacctgct gctggcgcac
     1021 ggcctggcgg tcagagcgtt cagggattcg tcaagccgca agcgagctgc aggcacagat
     1081 gcttctcagc cagtcgatgg aatcgggatc gcgctcagta tcaactatca ggagcctgct
     1141 accgacgatg aactcgatat cgccgccgca aagcgtgtag acggcttctg gaataggctc
     1201 tatctggatc cgctgttccg cgggcactac tccgaagacg tcctagcatg gctagcccag
     1261 gcaggagtat tgcccgacgc cctcgccagg ccgcggcctg aggaaatgcg gacaatcagc
     1321 caagagacgg acttccttgg gattaactac tacaccagga atcgagtggc atgggatccc
     1381 gccgatcgcc tgctcaatgc ctcagcaatt gccccgcgtc tgccggtcac cgccatggga
     1441 tgggaagtgt accctgaggg gctgttcaag gtgctcaagt gggtgagcga cgactatacc
     1501 aagattccgc tgttcatagc cgaaaacgga gccgcgttcg atgatccgct ccccgcggat
     1561 ggctccatca tcgacgacag cgcccgcata gactacctct cgtcacacat tgaggcggcg
     1621 cacaaagcgt gtcaacgcgg cgtcaacttg agaggttact acgtgtggag cctcatggac
     1681 aacttcgaat gggcccaggg cttctccaag cgattcgggc tagtgcatgt ggactttcca
     1741 acacagcaga ggacgtacaa gtgcagcgcc ctgtggtatc gggacctcat ccggtcacag
     1801 cggtaagtca atcagaatat ccggcgtccg tcgggaggga ttctcatttt ggcacagaat
     1861 gacctgtatt gatataccat ctttggcgtc cataccaatt tagtatgtaa ttcgccgggt
     1921 ccatatccat gtatgataac cctcggagcc attggtggta tgaattttga taaattgtaa
     1981 ggagggctcc gggtgaaacg gcatcgtcaa ggtctcttgc tcactcttgc gatgggaatg
     2041 ctcttggtca tcgcagtgac cgccaccggg tgcaatggta aggcctattc ggtctcgggc
     2101 cgggtgacca tcataggtac aggtatcggc attccaaacg cgacagtagc gtgcggcgtg
     2161 tacactgcct ttacggactc gtccggatac tggggcgtga gcggtctcgg tagtacaacc
     2221 actgtctcag taacgaaaga gggatggaag ttcgaacctg cgtcagttcg agtatacggc
     2281 gacaggaccg acgtcaactt tcaaggcagc gaactcgact accgaggcct gtcttccgac
     2341 tcgagtcaat ggtactacga tgtaggggta accgctaccg ggcagcccga tctgatcgga
     2401 tggatcaggc aacgtgtcac acgggtcgaa cgggagagtc gccgaactct gtttcacatg
     2461 cgctacgaaa ttggaggcga catgtttcga ggctccctta gagatctcgc tccgtggttc
     2521 ccgggcgccg gggccgcctc ggaggcctat gattacttca tagcccgtga aggcgcgact
     2581 tactatctgc tgccggaccg agactccaca cctatcttca tgttgaacaa accgctgtac
     2641 cagggagcca catacaatga catggtcata gaactgccct tcgagctcac agtacagaga
     2701 gaagaagagg tcaccgtgcc tgccggcact ttcagcacgt ggtactgcag cagattggca
     2761 acagacgatg gaatgaccta cctagccagg gcgtggtttg ctccgtacgt cgggcctgtc
     2821 aagtacatga tagaggtcac tgatactagc ggatctggaa tgtatggctg catcgtggtt
     2881 gccctgcgat catacaacga gtagaatgtt cgaacacgag gcgttctagg ttctggagcc
     2941 ctcggctgcc ccggacggtg gtgccgaggg ctctaccttg tctcgatctg caacatgaag
     3001 agcagca
//
LOCUS       DAOTAC010000297         2988 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_25863_length_2988_cov_3.794408, whole genome shotgun sequence.
ACCESSION   DAOTAC010000297 DAOTAC010000000
VERSION     DAOTAC010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25863_length_2988_cov_3.794408"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            239..1129
                     /locus_tag="PLM74_10610"
     CDS             239..1129
                     /locus_tag="PLM74_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019435257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HON43560.1"
                     /translation="MRVQCDNPIISRRTAAVFALPTLVFIGVFLAFPALWAMYIGLTN
                     LRLTGISAIQPRFVGLANFVSILRDSFFHNSLRITAQFVLGSALIGQLLLGLLLAVLL
                     YNVKGPINDFVSSIVILAWIIPEVVVAYLWVAFLDYDFGLLNATRNLLGMPAVNWFFS
                     HPVLSIIVFNTWRGTAFSMLLMGAALQTIPPSFLETADVIGASEWRKFRDTMLPLIRP
                     HIITNAVLITIWTFNVFGPFLLTGGGPAFATDLVSIYTYRNAFKYFKLGYGSAISTII
                     LAINLILASVYMKLARRGRA"
     gene            1132..1935
                     /locus_tag="PLM74_10615"
     CDS             1132..1935
                     /locus_tag="PLM74_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008546842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HON43561.1"
                     /translation="MKRVLSVVALLLVAALFALPILWLVTAPFNEKPTLGLQFAKPTL
                     RNFEGVFKNPTAVRGLRNSLLQAAATMLIATPVATMAAYAFSRASFPGQHTILYLLIL
                     LSSVISGVSAMVPLYVLMQTLGLIDKHVGLVMVYVGGLLPTNMFIMKDFVDSIPRAYE
                     EAALVDGSTPRQVLLNIAFPLCRPGMAVIAVLIFVSTWSNFLVPFILLRSASKAPASV
                     AIYTFFNEVGIPLIGLIAAYSFIYTVPVVILYLVVNRKFGFRFYGGIKG"
     gene            1998..>2988
                     /locus_tag="PLM74_10620"
     CDS             1998..>2988
                     /locus_tag="PLM74_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008546919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON43562.1"
                     /translation="MARVHLERVSKNFDKVQAVCDVTITISDGEFFTLLGPSGCGKTT
                     TLRMVAGLESPTSGQVFIGNRDVTKEPPKNRNVAMVFQNYALYPHMTVAENIGYPLRI
                     RNKPRAQMDKRVAEVAESLAIGDILGRRPSEISGGQQQRAALARAIIHEPDVFLFDEP
                     LSNLDAKLRADARTFLKDLQARMGITTVYVTHDQSEAMAMSDRVAVMDQGRVVQVGPP
                     LEVYRRPASVFVADFLGNPSMNLLHGTLTLHAGMPAVLLGAAAVPMPLIQPPGGIDRM
                     AAGDAVTLGLRPEDISIRIQGGEDGIPGEVHVVEPLGSETLVSVKTSVGSVKVK"
BASE COUNT          591 a          847 c          874 g          676 t
ORIGIN      
        1 gtcccctcta cccggaggtg tcgtaccaag tccagctcat gactgagcga gtggtcaccg
       61 ggcagatgac acccgaacag gcgctggacg cttacgctaa ggcggtcgcc gaaattgccg
      121 gagatgcgaa cgtcgagatt atgaaatagc ccgcatgatc ttgtgcttct gaagacaagc
      181 aaggccaggc gatggcccaa gggccttcgc ctggcccaag aaggggcgct gaatatcgat
      241 gagggtgcag tgcgacaacc ccatcatttc ccgccgaact gcggctgttt tcgcattgcc
      301 aacgctggtg tttatcggcg tgttcctggc gtttcccgcc ttgtgggcta tgtacatcgg
      361 gctcactaac ctcaggctca cagggatttc ggcgatccag cccagattcg tgggtcttgc
      421 caacttcgta tcgatactcc gcgactcctt cttccacaac tcgctccgga tcactgctca
      481 gttcgttctg ggctccgcat tgatcgggca acttctcctc ggcctgcttc tggcggtgtt
      541 gctctacaac gtgaaaggtc cgatcaacga cttcgtctcc agcatcgtga ttcttgcctg
      601 gataatccct gaagtcgtgg tggcatatct gtgggtggcg tttctcgact acgattttgg
      661 cttgcttaac gcgacgcgca acctcctggg catgcccgcg gtgaactggt tcttcagcca
      721 cccggtcctt tcgatcattg tcttcaacac gtggagaggc actgcgttct caatgcttct
      781 gatgggggca gccttgcaga cgatacctcc gtctttcctc gaaaccgccg acgtcattgg
      841 cgcgtccgag tggaggaaat tccgtgatac catgctcccg ctcatccgcc ctcacataat
      901 cacgaacgct gtgctcatca caatctggac gttcaacgta ttcggcccat ttctgctcac
      961 aggcggcgga ccggcgtttg cgactgacct tgtgtccata tacacataca ggaacgcttt
     1021 caaatacttc aagctcggtt acggtagcgc catatccaca atcattctgg ccataaactt
     1081 gatccttgcg tcggtataca tgaagcttgc tcgccggggg agggcataga catgaagaga
     1141 gtgctgtctg ttgtggcgct gcttctagta gctgcgttgt tcgccttgcc catcctgtgg
     1201 cttgttaccg cgccgttcaa cgaaaaaccc accctggggc tgcagtttgc gaagcccact
     1261 cttcgaaact tcgaaggggt gttcaaaaac cctactgcag tgaggggcct acgcaacagc
     1321 ctactgcagg cggcggccac catgctcatt gccacgcccg tcgcgactat ggcggcctat
     1381 gcgttttccc gggcatcatt tccgggccag catactattc tatacttact gatacttctt
     1441 tccagcgtta tatctggcgt gtcggccatg gttcctctct acgttctgat gcagactctc
     1501 ggactcatag ataagcatgt cgggctggtc atggtatatg tgggagggct tctgcccacg
     1561 aacatgttca taatgaagga cttcgtcgac tcaatcccca gagcttacga agaagcggcg
     1621 ctcgtggacg gaagcactcc gcggcaggtc ctgctcaaca tcgcgttccc gctgtgtcgg
     1681 ccggggatgg cggtgatcgc ggtcttgatc ttcgtgagca cgtggagcaa cttcctggtg
     1741 ccgttcatac tgctgcgatc cgcgtccaag gcgccggcgt cagtggccat atacacgttc
     1801 ttcaacgagg ttggaatacc gttgattggc ctgattgcag cctattcttt catatacacg
     1861 gtgccggttg tgatcctgta tctcgtcgtg aacaggaaat tcggcttcag gttctacggc
     1921 ggtatcaagg gctaggcaag aggaacggct aggcgtgatc atggacaaga ggtcgccaga
     1981 agctaggggg cgttgacatg gcacgggtgc atttggagcg agtgtcgaag aacttcgaca
     2041 aggtgcaggc cgtatgtgac gtcacaatca cgatttcaga tggagagttc ttcacactgc
     2101 tgggcccgtc gggatgtgga aagaccacga cccttaggat ggtggctggg ctggagtcac
     2161 ccacgtcggg acaggtgttc ataggcaatc gtgatgtcac gaaagagcct cccaagaaca
     2221 ggaatgtggc catggtcttt cagaactacg cgctctaccc gcacatgact gtggcggaga
     2281 acattgggta tccgctgaga atccggaaca agcctcgcgc ccagatggat aagcgcgtcg
     2341 ccgaggttgc cgagagcctc gcgataggcg acatactggg ccgtcggccg agcgagatca
     2401 gcggcggcca gcagcagagg gctgcgctcg cccgcgccat catccacgag cccgacgtgt
     2461 tcttgtttga cgaacccctg tcgaatctgg atgcgaagct gagggcagat gcccgcactt
     2521 tcctcaaaga tctacaggcc cggatgggga tcacaacagt gtatgtgaca catgatcagt
     2581 ctgaggcgat ggccatgtcc gaccgggttg ccgtaatgga ccagggcagg gtggttcagg
     2641 tcggcccgcc tcttgaggtg taccgccgtc cggccagcgt gtttgtcgcg gactttctgg
     2701 gcaacccctc catgaacctg ctgcacggga ctctcacact gcacgcgggc atgccggcgg
     2761 tgctattggg cgccgcagcg gtgccgatgc cgctgattca acctccgggg ggcatcgatc
     2821 gaatggcagc gggcgacgcg gtcaccttgg gcctgcgtcc ggaggacatc agcattcgca
     2881 ttcagggagg cgaagatggg attcccggcg aggtgcacgt cgtggaaccg ctgggaagcg
     2941 agaccctagt gagcgtcaag acctctgtgg gctcggtcaa agtgaagg
//
LOCUS       DAOTAC010000298         2951 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_26256_length_2951_cov_5.312500, whole genome shotgun sequence.
ACCESSION   DAOTAC010000298 DAOTAC010000000
VERSION     DAOTAC010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26256_length_2951_cov_5.312500"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="PLM74_10625"
     CDS             complement(<1..606)
                     /locus_tag="PLM74_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine aldolase family protein"
                     /protein_id="HON43563.1"
                     /translation="MDFIEMRSDTLTLPTDEMREAMRYAEVGDDVYGEDPTVSRLEEL
                     AAMKVGKEAAVFVPSGTMGNLLAVMTHCRRGDEILMESECHIYYYEVGGLSAVAGAVP
                     RLVKGANGVLDPGDVEAAIREENIHYSPAACLAIENTHNRGGGTVTPTDTMAALRRVA
                     DAHGLAIHLDGARIFNASLALGVDASDIAKHVDSVMFCLSKG"
     gene            855..2402
                     /locus_tag="PLM74_10630"
     CDS             855..2402
                     /locus_tag="PLM74_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HON43564.1"
                     /translation="MGDNDKLRNLNARKGKVLDGGGPKSIEKQRAAGKLTARERIERL
                     LDPGSFAELDMFVEHRCHDLGMDKRELPGEGVVTGYGSIDGRKVFIFAQDFTVMGGSL
                     GEMHARKICKVMDLAAKVGAPLIGINDSGGARIQEGVDALSGYGQIFYRNTISSGVIP
                     QISVIMGPCAGGAVYSPALTDFVFMVQGANMFITGPQVIKAVTGEDVSADELGGAATH
                     NRTSGVAHFMYDAEDDCFMAIRKLLSYLPGNNLEDPPVSACSEVATHANPQMREIVPP
                     ESNRAYDMRHVITGFVDDADFFEVHQYYAMNIIVGFARLGGRPIGIIANQPRVAAGCL
                     DINASDKAARFIRFCDAFNVPVVNLVDVPGFLPGVNQETGGIIRHGAKMLYAYSEATV
                     PKITLIVRKAYGGAYLAMCSRDLGADAVFAWPGAEIAVMGPDGAANIIFRREIDAAEN
                     PDEARKQKIEEYRSKFANPFVAAARGFVDDVIDPAETRARLLGALDMLSTKRESRPPK
                     KHGNIPV"
     gene            2418..2831
                     /locus_tag="PLM74_10635"
     CDS             2418..2831
                     /locus_tag="PLM74_10635"
                     /inference="COORDINATES: protein motif:HMM:NF016187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HON43565.1"
                     /translation="MTVSEDLLLGLQTLALGMVVTFVGLLILQGVMILMARFSKANGN
                     NNRPSNAPAMSGPAHVKNNPPATMEGPAAQSEHPGRPDEEVAAISAVMAVLTAESGAP
                     MQIRSVRPVGPVPAAASNIWGLAGRQEIMATRRRA"
BASE COUNT          641 a          849 c          880 g          581 t
ORIGIN      
        1 ccctttggac aggcagaaca tcacggagtc cacatgcttc gcgatatccg atgcatccac
       61 gcccagggca aggcttgcgt tgaatatgcg ggcgccgtca aggtgtatag ccagtccatg
      121 cgcgtccgca acccggcgga gtgcagccat ggtgtcggta ggcgtcaccg tgccgccacc
      181 cctattgtgt gtattctcaa tggcgaggca tgcagcagga gagtaatgta tgttctcttc
      241 cctgatggcc gcctcgacat cccccggatc gagcacacca tttgcgccct tcacgaggcg
      301 aggaaccgca ccggccaccg cggagagccc ccctacctcg tagtagtaga tgtggcattc
      361 cgattccatc agtatctcgt cgccacgcct gcaatgggtc atcactgcca gcaggttgcc
      421 catggtccca ctaggcacga atacggccgc ctccttgccc accttcatgg cagccaattc
      481 ctcaagtcta cttacagtcg ggtcctcacc gtaaacatca tcgcccacct cggcatagcg
      541 catcgcttcg cgcatctcgt cagtgggaag agtcagtgtg tcgcttcgca tttcgatgaa
      601 atccatatac aacaccacct taggccgtta tgagcggttc gacgtgccga ttgtgccgag
      661 tattgagtga tacgcgaaac atagtgggta ttcctgcagc ccgattacgt acgcaggtta
      721 tggcgacagt tgtctgcaaa gtgtataata ggtttggtgt gcctaggcca ttccgctgcc
      781 tcatcctggg gatataccaa tgcaacatgc agttcgcaca ggcctgttcg agcgattaag
      841 cgaggtgagt tttcttgggc gacaacgaca agctccgcaa tttgaatgcc cgaaaaggca
      901 aggtgctcga cgggggcggc cccaagagca tcgaaaaaca gcgcgcggcg ggcaagctta
      961 ccgccaggga gcgcatcgaa cgtctactgg atccgggaag ctttgccgaa ctcgacatgt
     1021 tcgtcgaaca caggtgccat gatcttggaa tggacaaacg ggaacttccg ggcgaggggg
     1081 tcgtcaccgg ctacggcagc atagacggcc gcaaggtgtt catcttcgct caggacttca
     1141 cggtcatggg cggctcactg ggagaaatgc atgcccgaaa gatctgcaag gtcatggacc
     1201 tggcagccaa ggtcggagcg ccgctcatcg gtatcaacga ttcaggcgga gcacgcattc
     1261 aggagggcgt tgacgcactc tcaggctacg gccagatctt ctacagaaac accatttcat
     1321 caggggtaat cccacaaatc tcggtgatca tggggccctg cgcagggggc gcggtctact
     1381 cccctgcact gacggacttt gtgttcatgg tgcagggcgc caacatgttc atcaccgggc
     1441 cacaggtaat caaggccgtc acgggcgagg acgtatccgc cgatgagctg ggcggggccg
     1501 cgacgcacaa ccgcacgagc ggcgttgcgc acttcatgta cgacgccgag gacgactgct
     1561 tcatggccat ccgcaagctc ctgtcatact tgcccggcaa caatctggag gaccctccgg
     1621 tatccgcctg ctccgaggtt gctactcacg caaatcccca gatgcgcgag atcgtaccgc
     1681 ccgagtcgaa tagagcgtac gatatgcgcc acgtgatcac gggtttcgtc gatgatgcag
     1741 acttcttcga agtacatcag tattacgcca tgaacataat cgtaggtttt gcccgcctgg
     1801 gcgggcggcc catcgggatt atcgccaacc agccccgtgt ggcagccggt tgcctcgaca
     1861 taaacgcgtc cgacaaggcc gcccggttca tcaggttctg cgacgcattc aacgtcccag
     1921 tcgtcaactt ggtggacgtg cccggctttc ttcccggcgt caatcaggaa acgggcggca
     1981 taatcaggca cggagccaag atgctgtacg cctactccga ggccacggtg cccaagataa
     2041 cgctgatcgt ccggaaggcg tacggcgggg catacctggc catgtgcagc cgtgacctgg
     2101 gcgctgatgc tgtattcgca tggcccgggg ctgagatcgc ggtcatgggc cccgacggcg
     2161 cggccaacat catcttccgt cgcgagattg acgcggccga aaacccggat gaagcgcgca
     2221 aacagaaaat cgaggagtac cgctccaagt tcgccaatcc cttcgtagct gcagcgcgcg
     2281 ggttcgtcga tgacgtgatt gatccggcgg agacgcgtgc caggctcctg ggcgcgctcg
     2341 acatgttgtc aaccaaacgg gagtcgcgtc ctcccaagaa acatggaaac atcccggtgt
     2401 gatgggaggc atgagcgatg accgtaagtg aggatctact gctcggcctg caaacgctgg
     2461 ccttgggcat ggtggttact tttgtcggcc tgctcatact gcagggtgta atgattctga
     2521 tggccaggtt cagcaaggcc aatggcaaca ataacaggcc atcgaatgcc ccggccatgt
     2581 cgggcccggc ccatgtcaag aataatcctc ctgccacgat ggaaggaccg gcagcccaat
     2641 cggagcaccc gggccgtcct gacgaggagg tagccgcgat ctcggcagtg atggctgtgc
     2701 tgacggccga gagtggcgcg cctatgcaga taagatcagt aaggccagtg ggtcctgtgc
     2761 ctgctgcagc atcgaacatt tgggggcttg cgggcaggca agagatcatg gcgactcgcc
     2821 gaagggcgta gcaaactacc tagtagggta atcagttcaa tcctgtttgg aggaggactg
     2881 ctaagtgagg aagtttcgtg ttcaggtaaa tgggaacatg tacgaggtgg aagtagaaga
     2941 aatcggcgga g
//
LOCUS       DAOTAC010000299         2940 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_26368_length_2940_cov_4.654419, whole genome shotgun sequence.
ACCESSION   DAOTAC010000299 DAOTAC010000000
VERSION     DAOTAC010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26368_length_2940_cov_4.654419"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..1080
                     /locus_tag="PLM74_10640"
     CDS             142..1080
                     /locus_tag="PLM74_10640"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HON43566.1"
                     /translation="MRRRSCAMREVGIMLTSFDQVLARAKEHESARVAVCAAQDREVL
                     EAVKMAAQIGMGEFTLVGDARRITELASDIGLDLSGIVVMDEPDVQRAARLAVGLVSA
                     GEAGILMKGLVPTADFLRAVLDREVGLRTGKLLSHVAVFKSPRYDRFFYLTDGAMMVA
                     PTLEEKVQIVENAVGVAHRLGNETPKVACVAAVEVVNPAMPETVEAAALAKMSDRRQI
                     KGCIIDGPLGLDNAVSMDSAQHKGVGGPVAGQADIILAPDIAAGNAIYKTMIYFGDIE
                     SAAVVAGARAPIVLTSRSDSPRARLLSLAVAIVTSK"
     gene            1096..2196
                     /gene="buk"
                     /locus_tag="PLM74_10645"
     CDS             1096..2196
                     /gene="buk"
                     /locus_tag="PLM74_10645"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HON43567.1"
                     /translation="MNDPTLLVINPGSTSTKVAIFQGERIEASDTLRHDAAAIAAFPR
                     IFDQFDMRRDAVYQFLADRSFPIEHLSAVIGRGGVLPPVEGGVYIVDDALVEALYEYG
                     WNHASSLGGLIARDIAGAVGIPAYIADPVTTDELDDIARITGLQEIERRSVFHALNQK
                     AVARRAASALGREYHECNLIVVHLGGGISVGGHRRGRVVEVSNALDGEGPYAPERSGS
                     LPNTGVVRLCFSGAYTEREVRKKITGKGGLVAYLGTNSLLDVENRIAAGDEYARLCYE
                     GMAYQVAKEIGRVACAMSGEVDAIVVTGGAANSTMLVGLIRDRVEFIARVLVYPGEEE
                     MQALALAAMRVLAGIEQAKRIAPLTAGRKSHS"
     gene            2225..2431
                     /locus_tag="PLM74_10650"
     CDS             2225..2431
                     /locus_tag="PLM74_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014183550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HON43568.1"
                     /translation="MSKVVFDEAKCKGCELCVHFCPKKVIRMADRINAKGYHPAYLAD
                     EDNCISCGICGRMCPDAVIEVFKP"
     gene            2446..>2940
                     /locus_tag="PLM74_10655"
     CDS             2446..>2940
                     /locus_tag="PLM74_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     beta"
                     /protein_id="HON43569.1"
                     /translation="MAERKLFKGNEAVAEAAVRAGCRFFFGYPITPQNEIPEYMSRRL
                     PEVGGVFLQAESEVAAINMVYGAAGAGGRVMTSSSSPGISLKQEGISYIAGAELPCLI
                     VNMVRGGPGLGGIQAAQSDYFQAVKGGGHGDYHCIVYTPASVQEMVDLTMQAFDVAEK
                     YRMPV"
BASE COUNT          607 a          779 c          994 g          560 t
ORIGIN      
        1 cgcctggggc aggctggtgg atgacgtgac agcgcgatgc tcattcatag cgccggtcat
       61 ggtgtttccc ggggagaacg agatggaggc cctggcctgg ggggctatgc gagtacttgc
      121 gggccaggaa cgagcgcggg aatacgacgt cgctcctgcg ctatgaggga ggtaggtatc
      181 atgctgacat cctttgatca ggttctggcg cgcgcaaaag aacatgagtc agcacgagtg
      241 gccgtgtgtg cagcgcagga ccgtgaggtt ctcgaggcag tgaagatggc cgcccagatc
      301 gggatgggcg agttcaccct ggtcggcgat gcccgccgga tcacggagtt ggcttccgat
      361 atcggcctgg atctatccgg aatagtcgtt atggacgaac cggatgtgca aagagctgcg
      421 cgcctcgcag tcggcctggt gagcgcaggg gaggccggca tcctcatgaa agggcttgtg
      481 cccacagccg actttctccg agccgtgctc gaccgcgagg tgggcctgcg cacaggcaag
      541 ttactgagcc atgtggccgt tttcaagtct ccccgctacg ataggttctt ctacctgact
      601 gacggcgcca tgatggtcgc ccctactctg gaagaaaagg tacagatcgt tgagaatgcg
      661 gtgggtgttg cgcacaggct gggaaatgaa acgcccaagg tggcgtgtgt ggccgcagtg
      721 gaggtagtga atccggctat gcccgagacc gtagaagcgg cggctctggc caagatgagc
      781 gacagacggc agataaaagg gtgcatcatc gatggtccgc tcgggctcga caacgctgtt
      841 tccatggatt cggcccagca caaaggtgtg ggagggcccg tggccgggca ggccgacata
      901 atcctggcac ctgacatagc ggcaggcaac gccatttaca agaccatgat ctatttcggc
      961 gacatcgaat ctgcagccgt ggtagcgggc gcccgagccc cgatagtgct tacttcgagg
     1021 tccgattcgc cgcgagccag gttgctttcg ctggctgtgg cgatagtgac gtcgaagtag
     1081 cgggaggtga ccgaggtgaa cgacccgaca ctgctcgtga tcaaccccgg gtccacgtcc
     1141 acaaaggtcg ccattttcca gggcgagcgg atagaagcgt ccgatacgtt gcgccatgac
     1201 gctgccgcaa tcgctgcatt tcccagaatc tttgaccaat tcgatatgcg tagagatgct
     1261 gtataccagt ttctggccga ccgttccttt cccatcgaac acctctccgc cgtcataggg
     1321 cggggtgggg ttcttcctcc ggttgaaggc ggagtctaca ttgtagacga tgcgctggtc
     1381 gaggcattgt acgagtacgg gtggaaccat gcgtccagcc tgggcggtct catcgccagg
     1441 gacattgctg gggccgtagg gattcccgcc tacatcgcag atccggtgac caccgacgag
     1501 ctcgacgata tcgcgcgcat cacggggctg caggaaatcg agcgcagaag cgtcttccat
     1561 gcgctcaacc agaaggccgt ggctaggcgt gctgcttccg cgcttgggcg cgagtatcat
     1621 gagtgtaatc tgattgtggt gcaccttgga ggcggaatca gcgtgggcgg gcaccgcagg
     1681 ggacgagtag tcgaagtgag caatgctctc gacggcgaag ggccgtacgc tcccgaacgg
     1741 tcgggcagtc tccccaatac cggagtagtg agactttgtt tcagcggcgc ctacaccgag
     1801 cgagaggttc ggaagaagat cacgggcaag ggcgggctag ttgcctatct gggcacaaac
     1861 tcgctgctgg atgtggaaaa ccggatcgcc gcaggtgatg agtacgccag gctatgctac
     1921 gaggggatgg cgtaccaggt ggccaaggag ataggccgag tcgcgtgcgc aatgagcggg
     1981 gaagtggatg ccattgtggt caccggaggc gctgctaact ccaccatgct ggtggggttg
     2041 atcagagatc gtgtagagtt catcgccagg gtgctggtat acccgggcga ggaagagatg
     2101 caggcactgg cgctggcggc catgagggtt ctcgcaggca ttgagcaggc aaagcgcata
     2161 gcgccgctaa cagccggccg gaagtcacac tcataatgaa gcaggaggag gcggcccgtc
     2221 tatcatgagc aaagtggttt tcgatgaggc gaagtgcaag ggctgcgagc tatgcgtgca
     2281 cttctgcccc aaaaaggtca tccggatggc cgacaggatc aacgccaagg ggtatcaccc
     2341 ggcatatctc gctgacgagg ataactgtat ctcgtgcggc atatgcggca ggatgtgccc
     2401 cgacgcagtc atcgaagtat tcaaaccctg aggagggcag tacgcatggc agaacgcaag
     2461 ctattcaaag gaaatgaggc cgtcgctgag gccgctgtca gggccgggtg ccgcttcttc
     2521 ttcgggtatc cgatcactcc gcagaatgag attccggagt atatgtcgcg gaggcttcct
     2581 gaggtgggcg gcgtgttcct tcaggccgag agcgaagtag cggccatcaa catggtgtat
     2641 ggcgccgcgg gcgccggcgg acgtgtgatg acgtcttcgt cgagccccgg aatcagcctc
     2701 aagcaggaag gaatatcgta catcgccggc gccgagctcc catgcctcat tgtgaacatg
     2761 gtccgtggag gaccgggcct cggcgggatt caagctgctc agagcgatta cttccaggcc
     2821 gtcaagggcg gagggcacgg cgactaccac tgtatcgtgt acacgcctgc atcggtgcag
     2881 gagatggtgg acctgaccat gcaggcattt gacgtggctg agaagtacag gatgccggtg
//
LOCUS       DAOTAC010000300         2934 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_26433_length_2934_cov_5.179229, whole genome shotgun sequence.
ACCESSION   DAOTAC010000300 DAOTAC010000000
VERSION     DAOTAC010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2934
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26433_length_2934_cov_5.179229"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..632
                     /locus_tag="PLM74_10660"
     CDS             78..632
                     /locus_tag="PLM74_10660"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HON43570.1"
                     /translation="MPVKRSSTGGALSWVVAVVVLGAVAVGAGWLIGQQALDWWNPDR
                     SSSAKTDSGDTGYSYYPWKDTSTDTSGQTASSGAAQTQTPAQTQAQTSTPVISLPQST
                     APASAPASSSETSRFYRVRVGSFATREAAMETAAELEAQGHPIFVTGGGPWSVQVGAF
                     SKRENAIALSDRLANQGYDVTIHE"
     gene            736..1113
                     /locus_tag="PLM74_10665"
     CDS             736..1113
                     /locus_tag="PLM74_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HON43571.1"
                     /translation="MKLEEIARLIRAEVLAGTDKLYTLDIDTACGADLMSDVLAFCHE
                     RTLLLTGLTSPQVVRSAEVAGLSGIVFVRGKKPGDDVVELANALGIPLLATDYPMFEA
                     CGMLYGVGIGGTYRLSRADGTGC"
     gene            1110..1559
                     /locus_tag="PLM74_10670"
     CDS             1110..1559
                     /locus_tag="PLM74_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HON43572.1"
                     /translation="MTASDEHALEMDFAVARHDFAAAGEVASRVKQVLRQLGITSDLV
                     RRATVATYEAEMNLVIHSSGGKIVLRVDSERIVVEACDIGPGIPDIELAMREGYSTAS
                     DEVREMGFGAGMGLPNMRRCSDSFTIDSSAGRGTTVTAIIYIAKPGS"
     gene            1590..2927
                     /locus_tag="PLM74_10675"
     CDS             1590..2927
                     /locus_tag="PLM74_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HON43573.1"
                     /translation="MESRFHSVKLLEDKCKGCTNCIKRCPTEAIRVREGKAVINAARC
                     VDCGECVRTCENHAKAIIANPLEDLQGFDYNIALPAPALYSQIGRASGPDEVVWALQQ
                     MGFDAVCDVSFGADIITECTRRYIKENTLPRPLISSACPAVVRLVQVRFPGLIPHLAK
                     MESPMYAAARVARARVGAQLRIEDERIGVFFISPCAAKITAIISPLGSEKSYVNGALA
                     VSDIYGQLIANLGRGGSSRPLYRGSGLGFGWAMNGGELRALGDMKALAVDGIHNVIGV
                     LEEADSGRLKRVDYIEALACQAGCVGGPANVENPFVARVRMRDVSAGIDAGSMAASLQ
                     LACDCIDEFGEDYFHMHEPIQPLAGMELAESLESAIARMGELERIVAELPGLDCGACG
                     SPTCRTHAEDVVCGRASETDCVFKLRERMQQMAEDLLRLARMDLPSMARRDDN"
BASE COUNT          578 a          818 c          980 g          558 t
ORIGIN      
        1 gttcggaata gatgtatcta ctgaggcggg gccatgcccc gcggcgcatg aactgagcta
       61 gaaggaaggg atctgttatg ccggtgaaaa ggtcatccac aggcggcgcg ctctcctggg
      121 ttgtcgcagt cgtcgtgctg ggtgcagtag cagtcggggc cggctggctg atcgggcagc
      181 aagccctaga ctggtggaac cccgaccgaa gctcgtctgc gaagaccgac tcgggagata
      241 cgggctatag ctactatccg tggaaggata cttcaactga tacctctggg cagactgcgt
      301 cctcaggcgc cgcccagacc cagaccccag cccagactca agcccagacc tcgactccgg
      361 tgatcagcct ccctcagagc accgcgccgg cgagcgcccc ggcgagcagc tcagagacga
      421 gcaggttcta ccgggtgcgt gtgggatcgt tcgccacgcg cgaggccgcg atggagactg
      481 cggcagagct tgaggctcag ggacatccca tttttgtcac cggaggcggg ccatggtcgg
      541 tgcaggtagg agcgttttcc aagagagaga acgccatcgc tctctcggat cgactcgcca
      601 accagggcta tgatgtgact attcacgagt aggcctgata gtccatggac gatacagccc
      661 ggccggctct gcgcaggccc gccggccggg tagggtgtcg cgcggcgcag cgctgtgctg
      721 atggaggcga tgcacttgaa actagaagag atagccaggc tgataagggc tgaagttctg
      781 gcaggtacgg acaagctata tactttggat atcgacaccg cttgcggcgc tgacttgatg
      841 agcgatgtgc tggcgttttg ccatgagcga accctgctgt tgaccggcct aaccagccct
      901 caggtagtta ggagcgccga ggtggcaggc ctgtcgggca tagtgttcgt ccgcggcaag
      961 aagccgggcg acgacgtggt cgagctcgcg aatgcgctgg gcattcctct gctcgccacc
     1021 gactacccca tgttcgaggc gtgcggaatg ctgtacggcg tcggcatagg cggcacgtat
     1081 cgtctttcgc gtgcggatgg gacaggctgt tgaccgcgag tgatgagcat gccctggaaa
     1141 tggactttgc cgtggcccgg cacgacttcg ccgcggcggg cgaggttgcc agccgagtca
     1201 agcaggtcct gcgtcagctg ggcatcacgt ccgatcttgt acggcgggca accgtggcca
     1261 cttacgaagc cgagatgaac ctggtcatac actcgagcgg gggcaagatc gtcctcaggg
     1321 tggattcgga gcgcatagtc gtggaggcgt gtgatatcgg ccccggaata ccagatatcg
     1381 agctggccat gcgcgaagga tattccacag cctctgacga agtgcgcgag atggggttcg
     1441 gcgccggtat gggcctgccg aacatgcggc gatgctcaga ttcgttcaca atcgattcca
     1501 gtgcgggacg cggtactact gttactgcaa tcatctacat agccaagccc gggtcgtgat
     1561 gatcgcgttc cagaggaggc ggtgcgaagt tggagagcag gtttcactcc gtcaaactcc
     1621 tcgaggacaa gtgcaagggc tgcacgaact gcatcaagag atgtccgacc gaggctattc
     1681 gagtgcgcga agggaaagcg gttatcaacg cggcgcgctg cgtagactgc ggcgaatgcg
     1741 tgcgcacatg cgagaaccac gccaaagcca ttatagccaa ccctcttgag gatctacaag
     1801 gattcgacta caacatcgcg ctgcccgccc ctgccctgta ctcacagatc ggcagggcct
     1861 ccgggccgga cgaggtagta tgggccctgc agcagatggg gttcgacgca gtatgcgatg
     1921 tgtcatttgg cgccgacatc atcaccgaat gcactcgccg ctacatcaag gagaatactc
     1981 tccctaggcc tctgatatcc agtgcctgtc cggctgtggt gcgcctggtt caggtgagat
     2041 tcccggggct gattccccat cttgccaaga tggagagccc gatgtacgcg gccgcccgcg
     2101 tagcccgggc gcgcgtcggc gcccagctca gaatagagga cgagaggatc ggagtcttct
     2161 tcatatctcc gtgcgctgcc aagatcaccg ccatcatctc ccccctggga tcagagaagt
     2221 catacgtgaa cggcgctttg gccgtgagcg atatatacgg ccagctcata gcgaatctcg
     2281 gccgcggcgg cagctcccgc ccgctttacc gtggctctgg gctcggtttt ggctgggcca
     2341 tgaacggagg cgagctgcgt gcgctgggcg acatgaaggc gctggccgtg gacggaatcc
     2401 acaatgtcat cggcgtgctt gaagaggccg acagcggcag gctgaagcgt gtcgactaca
     2461 tcgaggccct tgcgtgtcag gcgggatgtg tgggtggccc tgctaatgtg gagaatcctt
     2521 ttgtggcgcg ggtgcgtatg cgcgacgtgt cggcaggcat cgatgcggga tcaatggccg
     2581 catcgcttca gctggcgtgc gactgcatag atgagttcgg cgaggactac ttccatatgc
     2641 atgaacccat tcagccgctg gccggcatgg agctggcaga gagcctggaa tcggccatag
     2701 cccgcatggg agagcttgag agaatcgttg cggaactgcc tggcctggat tgcggcgcgt
     2761 gtggttcccc cacttgccgg acacatgctg aagatgtagt gtgcgggcgg gctagcgaga
     2821 cggactgcgt gtttaagctg cgagagagga tgcagcagat ggctgaggat ctgctgaggc
     2881 tggccagaat ggatctgccg tcgatggcga ggagggacga caattgactg tgca
//
LOCUS       DAOTAC010000301         2926 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_26505_length_2926_cov_4.502264, whole genome shotgun sequence.
ACCESSION   DAOTAC010000301 DAOTAC010000000
VERSION     DAOTAC010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26505_length_2926_cov_4.502264"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2680
                     /locus_tag="PLM74_10680"
     CDS             <1..2680
                     /locus_tag="PLM74_10680"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013985261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HON43574.1"
                     /translation="KITARKAEMVRIAERAFHLTEAYVLDPDGKEAKILGNGYLAPDI
                     KVITPADIVSAVNYLVNLQHGIGVTDDIDHLGNRRLKSVGELLQNQFRIGLSRMERVI
                     RERMTIQDVEAVTPQALINTRPVVAAVKEFFGSSQLSQFMDQTNPLAELTNKRRLSAL
                     GPGGLSRERAGFDVRDVHHSHYGRMCPIETPEGPNIGLIGSLCTYARINEYGFIETPY
                     RKVADGKATSEIRYLTADVEDRYTIAQANEPLNEDGSFVNPRVTCRLKEDIVIVSPSQ
                     VDFMDVSPKQLVSIATALIPFLENDDAGRALMGSNMQRQAVPLIRTEAPFVGTGMELK
                     AAVDSGVVVAAKRAGIVERVSADFVRVRRDDGDVDHYNLMKFARSNQGTCMNQRPIVR
                     VGQRVEVGDVLADGPSTDQGEMALGRNVLVAFMPWEGCNYEDAILISERLVSDDVFTS
                     IHIEEYESEARDTKLGPEEITRDIPNVGEDALKDLDHEGIIRVGAEVRTGDILVGKVT
                     PKGESELTPEERLLRAIFGEKARDVRDTSLKVPHGEAGKVVAVHVFSRENGDELGPGV
                     NKLVRVYVAQKRKISEGDKMAGRHGNKGVIAKILPVEDMPFLPDGRPIQIVLNPLGVP
                     SRMNIGQVLETHLGWASAALGVHIATPVFDGAREDDVFEFLEKADLPKNGKMALYDGR
                     TGERFDRQVTVGYAYIMKLMHLVDDKIHARSTGPYSLVTQQPLGGKAQFGGQRFGEME
                     VWALEAYGAAYTLQELLTVKSDDIAGRVKTYEAIVKGDNVPEPGVPESFKVLIKELQS
                     LCLDVKVLSDDEHEIEIREADEDIEGTARELGIDIGDRSSRIDRIGGPYRETEAADVG
                     LEDQGDDLGEDDGEEKDEDADSSDFDADDSDDDKID"
     gene            2712..>2926
                     /locus_tag="PLM74_10685"
     CDS             2712..>2926
                     /locus_tag="PLM74_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019225208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43575.1"
                     /translation="MLDVNDFERIRIAMASPEQIRGWSSGEVKKPETINYRTLKPERE
                     GLFCEKIFGPQRDWECHCGKYKRIRYKG"
BASE COUNT          673 a          756 c          948 g          549 t
ORIGIN      
        1 gaagatcacc gcgcgcaagg ccgagatggt gcggatagcg gagcgcgctt tccaccttac
       61 tgaggcctat gtgctcgatc ctgatggcaa agaagctaag atcctaggca acggctacct
      121 ggcgcccgat atcaaggtca ttacgcctgc cgacatcgtg tctgcagtca actacctggt
      181 caatctgcag catgggattg gcgtcaccga cgacatcgac cacctgggca accggaggct
      241 caagtcagtc ggcgaattgt tgcagaatca gttcaggata gggctgtcgc gcatggagcg
      301 tgtcatacgt gagcgcatga cgattcagga tgtggaggcc gtcaccccgc aggccctcat
      361 caataccagg cctgttgtag cggctgtcaa ggagtttttc gggtccagcc agctatcaca
      421 gttcatggac cagaccaacc ccctagcaga attgacgaac aagaggcgtc tgtcggcgct
      481 tggtcctgga gggctgtcga gggagagggc cggtttcgac gtgcgcgacg tgcatcactc
      541 tcactacggc aggatgtgcc cgattgagac acctgaaggg cccaacattg gcctgatcgg
      601 atcgctctgc acctatgctc ggatcaacga gtacggtttc atcgagacgc cttaccggaa
      661 agtcgcggat ggcaaggcta ccagcgagat ccgatatctc actgccgacg tcgaagatag
      721 atacacaatc gctcaggcaa acgagccgct gaatgaggat ggcagcttcg ttaaccccag
      781 agtcacatgt cgcttgaagg aagatattgt cattgtatcg ccctcgcagg ttgatttcat
      841 ggacgtgtcg cccaagcagt tggtgtcgat agcgactgcg ctcatcccgt tcttggagaa
      901 cgatgacgcc gggcgtgcgc tcatgggttc gaacatgcag cgtcaagctg tgccgctcat
      961 ccgaacggag gcgccgtttg tgggcaccgg tatggagctc aaggccgctg tagactccgg
     1021 agtcgtggta gccgccaagc gggccggcat cgtggaacgc gtctcggcag acttcgtgag
     1081 agtgcgcaga gacgacgggg acgtcgacca ttacaacctt atgaaattcg cccgttccaa
     1141 tcagggcacc tgtatgaacc agcggcctat cgtgagagtc gggcagcgtg tggaggtcgg
     1201 cgatgtgtta gctgacggcc catccaccga tcagggcgaa atggctcttg ggcgcaatgt
     1261 gctggtggct tttatgccct gggagggctg caactacgaa gacgcgatcc tcatatcgga
     1321 gcgcctggtc agcgacgatg tgttcacctc gattcacatt gaggagtacg agtccgaggc
     1381 gcgcgacact aagctcgggc cggaggagat aacacgcgat atccccaatg tgggtgagga
     1441 tgccctaaaa gacctggatc acgagggaat cattcgtgta ggtgccgaag tgcgcaccgg
     1501 cgatatcctc gtaggcaagg tcacgcccaa gggcgaaagc gagctcacgc cggaggagcg
     1561 gttgctcaga gcgattttcg gtgaaaaggc tcgggatgtg cgcgatacct ctctgaaggt
     1621 gccgcatggc gaggcgggca aggtagtcgc ggtgcacgtg ttctcacgtg aaaacggcga
     1681 tgagctgggc ccgggcgtca acaagctggt gcgggtgtat gttgcccaga aacgtaagat
     1741 ctcggaaggc gacaagatgg ccggccgcca cggcaacaag ggcgtaatag ccaagattct
     1801 accggtagag gatatgccgt ttcttcccga tggccgtcct atacagatag tcctgaaccc
     1861 gctgggcgtt ccgtcgcgta tgaacatcgg gcaggtgctc gagacgcacc tgggatgggc
     1921 atcggccgcg ctaggcgtgc atattgccac cccggtattc gacggagcgc gcgaggatga
     1981 cgtgtttgag ttccttgaga aagccgactt gccgaagaat ggcaagatgg cgctatatga
     2041 cggcagaacc ggcgaacgct tcgaccgtca ggttaccgtg ggctacgcct atattatgaa
     2101 actcatgcac cttgtggacg ataagatcca cgcccgctcc actgggccgt attcgctagt
     2161 tacccagcaa ccgctgggcg gcaaggcgca attcggcggg cagcgcttcg gcgagatgga
     2221 ggtgtgggcg ctggaggcgt atggcgcggc gtacactctg caggaactgc tcacagtgaa
     2281 gtccgacgat attgcaggac gcgtgaagac gtacgaagca atcgtcaagg gcgacaatgt
     2341 gcccgaacct ggagtgcccg agtctttcaa agtcctcatc aaggaattgc agagcctttg
     2401 ccttgacgtc aaagtgctat cagatgatga gcatgagatt gagatacgcg aggccgacga
     2461 agatatcgaa ggcactgcaa gggaactcgg gatcgacatc ggcgacaggt ccagcaggat
     2521 cgaccggatc ggcgggccgt acagggagac ggaagcggct gatgtgggtc tggaagatca
     2581 aggtgacgac ttgggcgagg acgacggaga ggagaaggat gaggatgccg actcctccga
     2641 ctttgatgct gacgacagtg atgacgacaa gatagattag gagcccgagg gcgaagaagg
     2701 gggagaggcg gatgctggac gtcaatgatt tcgagcggat caggatagcg atggcatccc
     2761 cggaacagat acgcggatgg tcgagcggcg aggtgaagaa gcctgagacc ataaactacc
     2821 gcactctcaa gcccgaacgc gagggactct tctgcgagaa gatattcgga ccccagcgtg
     2881 actgggagtg ccattgcggg aagtacaagc ggatcaggta taaggg
//
LOCUS       DAOTAC010000302         2881 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_27025_length_2881_cov_4.141543, whole genome shotgun sequence.
ACCESSION   DAOTAC010000302 DAOTAC010000000
VERSION     DAOTAC010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27025_length_2881_cov_4.141543"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="PLM74_10690"
     CDS             <1..519
                     /locus_tag="PLM74_10690"
                     /inference="COORDINATES: protein motif:HMM:NF020772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HON43576.1"
                     /translation="LAHQSEGVKLATCSQILAGAGATPRERIEPLESSWGVNCDHSVW
                     MNGRTKPMWEKIWELEDVVNGSWVLTPFGPWPGDFWDLQGHVDEFLREFLLMLASDWE
                     FLMYTGTAGDYPERRFEGHRRRCAEAYARLQRVLSDEDLPAYTYEPSAPAAPAGSAEY
                     GQDDSESTGKCE"
     gene            557..>1505
                     /locus_tag="PLM74_10695"
     CDS             557..>1505
                     /locus_tag="PLM74_10695"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HON43577.1"
                     /translation="MRVLFASSEVNPYMKVGGLADVAGSLPRFLAQAGCDVHLVMPKY
                     ETAKWNGLSFEEWGSVYPPMGCGREEGQVGRVEIAPNLTLYTIRNGAYFAREKPYDSP
                     DDMRRFMFFAKASYELARQLDVDIVHANDWHTGLLPVYCRVYGCPGDPGTVITIHNLA
                     FQGTGDWDDFIYSSLPWDHFGPSGAEFFGKFNILKAAIMYSDRISTVSPTYAHEIQTR
                     EFGMGLHEVLGARHEVIRGILNGIDYDEWNPMTDTLLTANYDAERIQAREENKKALLA
                     RFGLEYRPGAPVFGFVGRLFEQKGVDILIEAASRMADSDV"
     assembly_gap    1506..1515
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1516..1935
                     /locus_tag="PLM74_10700"
     CDS             <1516..1935
                     /locus_tag="PLM74_10700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HON43578.1"
                     /translation="EYEEALRLLAGARPGKIGIKLAFSNYLAHEIYAGADFFLMPSRF
                     EPCGLGQMISMRYGSIPVVRAVGGLRDSVHEPDTGFVFWEYSVHELYAAMQRALAVFR
                     DPEAMLAMRIRCMTADYSWGRSTHDYLAMYHEIAPAR"
     gene            1986..>2881
                     /gene="galT"
                     /locus_tag="PLM74_10705"
     CDS             1986..>2881
                     /gene="galT"
                     /locus_tag="PLM74_10705"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HON43579.1"
                     /translation="MSELRKDPITRRWVIIATERAKRPSQLSSPSPVTPPDMPARDPK
                     CPFCEGNEAMTPPEVAAYRHAGSQRDTPDWWIRVIPNKFSALDPDTPLERIGEGMYDM
                     ATGFGVHEVIVESPRHNHTWGTATDKEFEEIFWSYRDRLINLMRHPSLRYVLIFKNYG
                     KEAGASQPHGHSQLIGLPVVPKRVLEELESSEDYFRYKERCPLCDVIRQERADGRRII
                     HESEHFIAFAPYASYLPFQIMIMPKHHDPFFERMEKGQFMDLARVFRRVFWALAQVLG
                     DPPFNCVLHTTPPASAGDERYN"
BASE COUNT          592 a          808 c          902 g          569 t
ORIGIN      
        1 cttgcgcacc agtcggaagg cgtcaagctg gccacgtgtt cgcagatact cgcaggcgca
       61 ggcgcgaccc cgcgggagcg gatcgagccg ttggagtcgt cgtggggagt gaactgcgat
      121 cactcggtgt ggatgaacgg gcggaccaag cccatgtggg agaagatctg ggaactggag
      181 gacgtcgtca acggcagctg ggtgttgacg ccgtttgggc catggcccgg agatttttgg
      241 gacttgcagg gccatgtgga cgagttcctc cgcgagttcc tgctaatgct ggcttccgac
      301 tgggagtttc tcatgtatac tgggactgcc ggcgactatc cggaaaggcg gttcgagggc
      361 cacaggcgca ggtgcgcgga ggcctatgcg cgtcttcaga gggtcctgag tgatgaagac
      421 ctcccagcct acacctacga accctcggcg cccgccgcgc ctgccggctc cgccgagtat
      481 gggcaggacg attctgagtc gacaggcaaa tgcgaatagt gcggtaacaa cagctacatg
      541 aggaggtatg cttcgcgtga gagtgctctt tgcttcttca gaggtcaacc cgtatatgaa
      601 ggtgggcggc ctggcggacg ttgcaggaag tctgcccagg tttctggctc aggctgggtg
      661 cgatgtacac cttgtgatgc ccaagtacga gaccgcgaag tggaacgggc tgtcttttga
      721 ggagtgggga tcagtctacc cccccatggg atgcgggcga gaggaagggc aagtcggacg
      781 cgtcgagatt gctcccaacc tcacgctcta cactatccgc aatggcgcat actttgcccg
      841 cgaaaagcca tatgactcgc ccgacgacat gaggcggttc atgtttttcg ccaaggcctc
      901 gtatgagctg gcgcggcagc tcgatgtgga catcgtgcac gccaatgact ggcacactgg
      961 ccttctgccg gtctactgca gggtatacgg atgtccgggc gaccccggaa ctgtcattac
     1021 cattcacaac ctggccttcc agggtaccgg cgactgggat gacttcatct actcgtcctt
     1081 gccgtgggac cacttcggcc cgagcggggc cgagtttttc ggcaagttca atatcctaaa
     1141 agccgcaatc atgtattctg atagaatcag caccgtatcg cccacttacg cccacgagat
     1201 ccagacgcgc gagttcggaa tgggtctcca tgaagtgctg ggcgcaaggc atgaggtgat
     1261 ccggggcata ctcaacggca tcgactacga tgaatggaac ccgatgaccg acaccctgct
     1321 cacggccaac tatgacgcgg agcgcatcca agcgcgcgag gagaacaaga aggccctcct
     1381 ggccaggttc ggcctagaat acaggcccgg agcgcctgtg ttcggcttcg tggggcgtct
     1441 gttcgagcag aagggtgtag acattctgat cgaagccgca tcgcggatgg cggattcaga
     1501 tgtgcnnnnn nnnnngagta cgaggaagca ctacggcttc ttgccggcgc gcgcccaggc
     1561 aaaatcggca tcaaactggc atttagcaac tatctcgccc atgagatcta cgcgggggcc
     1621 gactttttcc tcatgccgtc gaggtttgag ccgtgcggac tggggcagat gatatccatg
     1681 agatacggct cgattcccgt ggtgcgggca gtcggcgggc tgcgcgattc agtgcacgag
     1741 cccgacactg gcttcgtgtt ctgggaatac tcagttcatg agctgtatgc agccatgcag
     1801 cgtgccctgg cggtgttccg agaccctgaa gcgatgctcg ccatgcgcat acgatgcatg
     1861 acagctgact attcgtgggg gcggtcgacc cacgattacc ttgcgatgta ccatgagatt
     1921 gcgccggcac gctagcgatg ctgcaggtgc gaccggtcta cattctgtgc tgaagaggtg
     1981 agccaatgtc cgaactaagg aaggacccca tcacccgcag gtgggtgatc atcgcaacgg
     2041 agagagccaa aagaccaagc cagctctcaa gcccgagccc ggtaacccct ccggacatgc
     2101 ctgcgcgtga cccgaaatgc ccgttctgcg agggcaatga ggcgatgacc cccccggagg
     2161 tggcagccta caggcatgca ggaagtcagc gtgatacgcc cgattggtgg ataagggtga
     2221 tccccaacaa gttctcggcg ctcgatcccg acacgccgct ggagcgtata ggcgaaggca
     2281 tgtacgacat ggcaaccggg ttcggcgtgc acgaggtgat cgttgagtcg ccgcggcaca
     2341 accatacttg ggggacggca accgacaagg agttcgagga gatcttctgg tcgtatcgcg
     2401 acaggctgat caatctcatg cgccatccgt ctttgcgcta cgtgctgatc ttcaagaact
     2461 acggcaaaga agcaggcgcg tcgcagcctc acggccattc gcagttgatt ggtcttcctg
     2521 tggtgcccaa gcgagtgctg gaggagctgg agagttccga agactatttc cgttacaagg
     2581 aacgctgccc gctatgtgat gtgatcaggc aggagcgtgc agacggcagg cgcatcatac
     2641 acgagtcaga gcattttatc gcattcgcgc catatgcctc gtatctgccg tttcagatca
     2701 tgatcatgcc caagcatcat gatcccttct tcgagcgaat ggagaagggc cagttcatgg
     2761 acctcgcacg cgtgttcagg cgtgtgttct gggcgctcgc tcaggtgctg ggcgacccgc
     2821 cgttcaactg cgtgctgcat accacgcctc cggcgtccgc gggagacgaa cgctacaacc
     2881 a
//
LOCUS       DAOTAC010000303         2858 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_27271_length_2858_cov_4.735640, whole genome shotgun sequence.
ACCESSION   DAOTAC010000303 DAOTAC010000000
VERSION     DAOTAC010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27271_length_2858_cov_4.735640"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1036)
                     /locus_tag="PLM74_10710"
     CDS             complement(<1..1036)
                     /locus_tag="PLM74_10710"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HON43580.1"
                     /translation="MEDVLLVGVDIGTSSTKGVLVDESGQIIASHTVPHGVVRPDPAW
                     AEQDADQVWWGDFAEIARALMSQAPAGSKVAGIGVSGTCPTLVPVGHDGKPLRRGILY
                     SIDRRALAEIDEIVSDVGEHEIVERSGNVLSTQCIAPKLLWLKRHEPEVYANTQWVLG
                     TTGYVVWRLTGEACCDHFCAGDGGYGYHLTDCSWDIDALSKMGIDPSILPPLKWATEV
                     VGAVTREAACETGLPEGTPVIAGTGDAAAEMVGTGVIDEGSLALLYGSTLATMSPVSR
                     AWLHPGFILTPGLVPGSYLVSSVLGTGSALVEWIRGTLAWDGSMPDHAALEGEAAVVP
                     AGSNGLVFIPY"
     gene            complement(1059..2252)
                     /locus_tag="PLM74_10715"
     CDS             complement(1059..2252)
                     /locus_tag="PLM74_10715"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HON43581.1"
                     /translation="MAIREGRALWVESQQDGILSGHYGPEDVKKIVDNAKSIGANMLL
                     WLVKARMADTAPALWYPSEIGLSHEDCYKYDSFGDVVRMAHEAGIEVHAYFSVFTEGD
                     IKAEAVKGKKPPLAEHPEWAVVDRLGKRSEFVCASHVGYREYLGSLVKEVLEKYPVDG
                     INLDFIRYPRSACFCDNCQTNIKERYGLTLDEVVDLLAGKGFDSLNYEDLGTALDRIG
                     AVINYYNQNVHETVEYIHGIVKGHSPKVKLSAAVFPNPRTAPSQVYCDWIGFSKFLDF
                     VCPMVYWYSPEYYRQTVGRLQSAMREGTKLYPGISALGVPHPLAGQNCNFLPGAPDMK
                     YVAELVDIAREVGAPGVAIFQYGTIFGLQPGAYTGVKWGTPVAGDVGLLRERFAQPAI
                     PAHAD"
     gene            complement(2381..>2858)
                     /locus_tag="PLM74_10720"
     CDS             complement(2381..>2858)
                     /locus_tag="PLM74_10720"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HON43582.1"
                     /translation="LERLNSVTQIVQGAGMKPGTAFRDWRSEMANSLIAQRLGIAIGD
                     PVSVIERVRTADGIPFCYDINFIPAKYITQQDEDSIGESLLRYLSERYGQIRQALAYL
                     YPYTADAIVSEKLGVPVGHLLMLLEHTHYTPDGSPMWYSRTFHRSDVISFHIVRSL"
BASE COUNT          572 a          898 c          770 g          618 t
ORIGIN      
        1 gatacgggat gaacaccagc ccgttgctgc ccgcgggcac gacagccgcc tcgccctcta
       61 gggcggcgtg gtcgggcatg gatccgtccc atgccagcgt tccgcgaatc cattcaacta
      121 gggcagatcc tgttccgagc acactgctca ccaggtaact gcctggcacg aggcccggag
      181 tgaggatgaa accggggtga agccacgccc gtgacactgg agacatagtt gccagagtgg
      241 agccgtatag cagggccagt gaaccctcgt cgataacgcc tgtgcccacc atctcggccg
      301 cggcgtcgcc tgtgcctgca atgaccggcg tgccttccgg caagcccgtc tcgcaggccg
      361 cctcccgggt cactgcgccc acaacctcag ttgcccactt gaggggaggg aggatggagg
      421 gatcgatgcc catcttgctc agggcatcga tatcccaact gcaatcagtg aggtggtatc
      481 catagccgcc atcgccggcg cagaaatggt cgcagcacgc ttccccggta aggcgccata
      541 caacgtatcc cgtcgtaccg agtacccatt gggtatttgc atacacctcc ggttcgtgcc
      601 gcttcagcca tagcagcttg ggagcaatac actgggtaga gaggacattg ccgctgcgct
      661 ccacaatctc atgttcgcct acgtccgata cgatctcgtc gatttctgca agtgctcgac
      721 ggtcgatgct gtatagaata cctcgacgaa gcggctttcc atcatgacct accggcacca
      781 gtgtagggca tgtgccgctc actccgatgc ctgctacttt cgagcccgcc ggcgcctgcg
      841 acatgagcgc gcgcgcaatc tcagcaaagt ctccccacca tacttggtca gcgtcctgct
      901 cagcccatgc ggggtcaggc cttacgaccc cgtgaggtac agtgtgtgat gcgattatct
      961 gccccgactc gtcgaccagg acacccttag tcgacgatgt gcctatatct accccgacca
     1021 gtagcacgtc ttccatcctc caaacctcgt agcgtcgcct agtcggcgtg cgccggtatg
     1081 gccggctggg cgaacctttc acgcagcagc cccacatcgc cggccaccgg agttccccat
     1141 ttcaccccag tgtaggcgcc cggctgcaag ccgaatatcg ttccgtattg gaatatcgca
     1201 actcccggag cacccacctc gcgagctatg tcgacgagct cagcgacgta cttcatgtcc
     1261 ggggcgcccg gcaggaagtt gcagttctgg cccgccagcg gatgcggcac gccaagcgcg
     1321 gatatcccag gatatagctt tgtgccctcg cgcatggcgc tctggagcct gccaaccgtc
     1381 tgtctgtagt actcgggcga gtaccagtac accatcgggc acacgaagtc caggaacttc
     1441 gagaacccta tccagtcgca gtagacctgg ctcggggccg ttcttgggtt ggggaacacg
     1501 gccgccgaca gcttgacctt gggcgaatgg cccttgacga tgccgtgaat gtactcaacg
     1561 gtctcatgaa cgttctggtt gtagtagttg atgactgcgc cgatcctgtc gagggctgtt
     1621 ccaagatcct cgtagttcag cgaatcgaag cccttgccag ccagcaggtc cactacctca
     1681 tcgagagtaa gcccgtagcg ctccttaatg ttcgtctggc aattgtcgca gaagcaggcg
     1741 gaacgaggat agcggatgaa gtcaaggttg atcccgtcca ccgggtactt ctcgaggacc
     1801 tcttttacca gtgaaccaag gtattcgcgg tatcccacgt gacttgcgca tacgaattcg
     1861 ctgcgcttgc ccagtctgtc cacgaccgcc cattcgggat gctctgccag tgggggtttt
     1921 ttccccttca cggcttctgc ctttatatca ccctcggtga atacggagaa gtaggcatgg
     1981 acctcaatgc cggcctcatg cgccatcctg actacgtctc cgaagctatc gtatttgtag
     2041 cagtcttcgt ggcttaggcc aatttcgcta ggataccaaa gggccggcgc cgtgtcggcc
     2101 atgcgagcct taacaagcca gaggagcatg ttagctccga tgctctttgc attatcaacg
     2161 atctttttca catcttcagg accatagtgc ccgctcagaa tgccgtcctg ctgtgattcc
     2221 acccagagag cccgtccttc tctgatcgcc attcagattc catccccttc cacaatagtc
     2281 tggtgagtag catatctgca tactaatctg cgacgtcttc taccctgatt gctcacccct
     2341 ttacagttcc acctggccct ccggtgaacc cactgcccga tcagagcgat ctgacaatat
     2401 ggaagctgat cacatcactc cggtggaacg tcctggagta ccacatggga ctcccgtcag
     2461 gagtgtagtg ggtatgctca aggagcatca gtagatgccc cacggggact cctagtttct
     2521 cgctgactat ggcgtcggcc gtgtacgggt ataagtaggc caaggcctga cgtatctgac
     2581 cgtatcgctc cgacaagtag cgtagcaacg actcgccgat gctatcctca tcctgctgag
     2641 tgatgtactt ggcgggtatg aagttgatat cgtaacagaa cggaatgccg tcagcagtac
     2701 gcacgcgctc gatcaccgaa accggatctc cgatcgctat gccgagtctt tgcgctatga
     2761 ggctgttggc catctcgctc cgccaatccc gaaaagcggt tcccggtttc atgccggcgc
     2821 cctgcacaat ctgagtgacc gaattcagcc tctcaagc
//
LOCUS       DAOTAC010000304         2856 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_27303_length_2856_cov_4.469832, whole genome shotgun sequence.
ACCESSION   DAOTAC010000304 DAOTAC010000000
VERSION     DAOTAC010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27303_length_2856_cov_4.469832"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..987)
                     /locus_tag="PLM74_10725"
     CDS             complement(<1..987)
                     /locus_tag="PLM74_10725"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HON43583.1"
                     /translation="MDPRVEKLAEILVDYSVGVRKGDVVMVGADVDGAPLAKAVYRRV
                     LQRGGFPRLRVYLPEVTRIYFEEASDEQLGTLLPIDLDEYEASQCLIRISAPANLAET
                     ANVDPGKQSARRRTMKPVSDYVMAGKVRWCGCNFPCNALAQKAEMSLEEYEDFVYGAT
                     NVDWAALSREQEAIKQLFDGGRQVHLIGEGTDLTFSIEGRPGVNCDGHYNMPDGEVFY
                     SPVEDSAEGYITFSYPAIYMDREVEGVRLTFSQGKVVEARAAKNEEFLLKMLDTDPGA
                     RRIGEFGIGTNHGITKFTRDILFDEKIGGTIHLALGQSYPEAKGENDSAIHWD"
     gene            1307..>2856
                     /locus_tag="PLM74_10730"
     CDS             1307..>2856
                     /locus_tag="PLM74_10730"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HON43584.1"
                     /translation="MQQSESQGERQAEYRGFKLDPFQVEAIRHLNEGRSVLVSAPTGV
                     GKTLVADYLIERMFHEGRRVIYTAPIKALSNQKFKEFKRLLGAGNVGIVTGDVAINST
                     AQILIMTTEIFRNMLHLSPEALGGVSHVIFDEIHYLSDEERGTVWEESIIFAPDSMRL
                     LGLSATIPNADELAFWIQDIKKHEVAVVRHFKRVVPLRHFVYEHTRGACTLAELKEFK
                     SRRDREIEESDELWEPEGPRYATTTHIDLVDYLSKQRKLPCLYFVFSRRQCEEKAEEL
                     GRLVNYLTPGERNQVLTFFDDRIEGMNIGSMPSVKRMRKTLERGIAYHHAGLLPVLKE
                     IVEDLFERKIIRVLYATETFAVGINFPVRTVCFDSVRKFNGVDFRPMTAQEYFQMAGR
                     AGRRGIDEEGYVYILTDLNYFRPEEYPSTDERTVEPLVSRFTVSYNTVVNLVATRSPE
                     EIHRVLKQNFASYQTNAERHEIEAGLVTARELESEIVGGACERLNQAGCPLYASGLNA
                     RVADLRLQL"
BASE COUNT          601 a          829 c          843 g          583 t
ORIGIN      
        1 gtcccagtga atcgctgagt cattctcacc tttggcctcc ggatacgact ggcccagagc
       61 tagatggatt gttccgccta tcttctcgtc gaaaaggata tcccgggtga atttggtgat
      121 cccgtgatta gtgccgatgc cgaactcacc gatgcggcgg gcgcccgggt cagtatcgag
      181 catcttcagg aggaactcct cgtttttcgc ggcgcgcgcc tctaccactt tcccctggga
      241 gaacgtgagg cgtacccctt ccacctcgcg gtccatgtag atcgcaggat agctgaaggt
      301 tatgtagccc tcggccgagt cctccaccgg ggagtagaac acctctccgt ccggcatgtt
      361 gtagtgcccg tcgcagttta ctccggggcg gccttcgatg gagaatgtca ggtccgtgcc
      421 ctcgccgatt aggtgcacct gtcggccgcc gtcgaatagc tgcttaatcg cttcctgctc
      481 acgcgagagc gccgcccagt ccacattggt ggcgccgtac acgaagtctt catattcctc
      541 cagcgacatc tcggccttct gtgccagagc gttgcagggg aagttgcacc cacaccagcg
      601 tactttgcca gccatcacgt agtcggagac gggtttcata gtccggcgtc tcgccgactg
      661 cttgccagga tccacattgg cagtctcggc caaattcgcc ggggcggaaa tccgaattag
      721 gcactgcgat gcctcatact cgtccagatc gatggggagc aaggtgccca actgctcgtc
      781 tgaggcttct tcgaagtaga tgcgggtgac ctcgggcagg tataccctga gccgcgggaa
      841 tcctccgcgc tgcaaaactc gccggtacac tgccttcgcc agcggtgcgc cgtccacatc
      901 cgcgcccacc atcaccacat cgcctttgcg cactcccacg gagtaatcca ctagtatctc
      961 ggccagcttt tcgactctcg ggtccatcca cacaacctcc cacaataccc actacacagt
     1021 cattcagaac ccacacgcac tacaattctg ggtttggaag gcgaatcctg ccatcgggtc
     1081 gcgcgcccta tcactggtta ggcatcgctg gtctggcatc gccgggcagg ttcatccgcc
     1141 tggcataacc cgcctgggtg attgtgccac accgcctgaa ccggtatact catcatagcg
     1201 gaagcgagat ccgtgcagcg cagagcagat cgtgcacgtt aggtcgccta cggtgtagga
     1261 tgtgggctag aatataaaca caaaaagaca gacaaggggg aagtcgatgc agcagagcga
     1321 gtcccagggt gagcgccagg ctgagtaccg cggtttcaag ctggacccgt tccaggtgga
     1381 agcgatccga catctgaatg agggccggtc ggtgctggta tcggcgccga ctggggtggg
     1441 caaaacactc gtcgccgact atctgattga gcggatgttc cacgagggcc ggcgggtcat
     1501 atataccgca cccatcaagg ctttgagtaa ccagaagttc aaggagttca agcgactttt
     1561 gggcgcgggc aatgtgggta tagtcactgg cgatgtggcg attaactcta cggcgcagat
     1621 cctgataatg acgactgaga tattccggaa catgctccat ttgagtccgg aggccttggg
     1681 cggagtgtcg cacgtcatat tcgacgaaat ccactatctg tcggatgaag aacggggtac
     1741 ggtgtgggag gaatccatca tctttgcgcc cgactccatg cgcctattgg gcctttcagc
     1801 cacgatcccc aacgctgatg agctggcttt ctggatacag gacatcaaga aacacgaagt
     1861 cgcggtagtc aggcacttca agcgcgtggt gccgttacgg cactttgtgt atgagcacac
     1921 tcgcggcgcg tgtacgttgg cggagcttaa ggaattcaag tccaggcgcg acagggaaat
     1981 cgaggaatcg gacgagcttt gggaaccgga agggcctagg tacgcaacga ccacgcatat
     2041 cgacctcgtc gattacctgt ccaaacagcg taagctgccg tgcctttact tcgtgttcag
     2101 ccggaggcag tgtgaggaga aagccgagga gctgggccgc ctggtaaact acctcacgcc
     2161 aggcgagcgg aaccaggtgc tgaccttctt cgatgatcgg atcgaaggca tgaacatcgg
     2221 ctccatgccc agtgtgaagc ggatgcgcaa gacgctggag cgagggatag cgtaccacca
     2281 tgccggcctg ttgccggtgc ttaaggagat tgtggaggac cttttcgaac gcaagatcat
     2341 ccgtgtgctt tacgctacag agacctttgc ggttggaatc aacttccctg ttaggactgt
     2401 ctgttttgac agcgttcgca agttcaacgg ggtggacttc cgccccatga cggctcagga
     2461 gtacttccag atggcagggc gcgcaggcag gcgggggata gatgaagagg gctatgtgta
     2521 catcctcact gacctcaact acttccgccc cgaggagtac ccaagcaccg atgagcggac
     2581 ggtcgaaccc ctggtgagca ggttcaccgt gtcctacaac actgtcgtaa acctggtggc
     2641 cacgcgttct cccgaggaga ttcatagagt gctcaagcag aacttcgcca gctaccagac
     2701 gaacgcggag cgccacgaga tcgaagcagg actagtcacc gcgcgcgagc tggagtcgga
     2761 gatcgtgggc ggcgcgtgcg agcggctgaa ccaggccgga tgccccctct atgccagcgg
     2821 gctcaacgct cgggtcgccg acctcaggct gcagct
//
LOCUS       DAOTAC010000305         2836 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_27549_length_2836_cov_4.208198, whole genome shotgun sequence.
ACCESSION   DAOTAC010000305 DAOTAC010000000
VERSION     DAOTAC010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27549_length_2836_cov_4.208198"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="PLM74_10735"
     CDS             complement(<1..680)
                     /locus_tag="PLM74_10735"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HON43585.1"
                     /translation="MSNSSFKAAPVAWLAQVSQVVNEALDALMPSGGTPPQALHAAMR
                     YSVFPGGKRLRPALAVAAFEAVHTERGAPGVSPDGRRALAVRAGAAVELIHCYSLIHD
                     DLPCMDDDDFRRGRPSCHRTYGEAVALLAGDALLTLAFESLALPEFVEAAGAHSACLL
                     ASELAQASGSLGMAGGQALELAISQQSWSGPLCEAAEAVQRMESMKTGRLFEAAARMG
                     GIVGGASGL"
     gene            complement(677..934)
                     /gene="xseB"
                     /locus_tag="PLM74_10740"
     CDS             complement(677..934)
                     /gene="xseB"
                     /locus_tag="PLM74_10740"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HON43586.1"
                     /translation="MKAELTFEKALARLEQIVAQLDAGDLPLDEALELFGEGSELGKF
                     CSAILDQAEGRLEMLIEGKDGKPEVAPAQGMLGKLEGENGD"
     gene            complement(931..2100)
                     /gene="xseA"
                     /locus_tag="PLM74_10745"
     CDS             complement(931..2100)
                     /gene="xseA"
                     /locus_tag="PLM74_10745"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011877433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HON43587.1"
                     /translation="MNAYGLEIEVYSVSQITDRVTRLLQADAVLADVWVSGEISNFTH
                     HSSGHMYMTLKDDASRLRAVMFQGANRGLRFRPESGMRVFAHGYVGVYRSGGEYQLYI
                     DFMEPAGLGGLFLAFQQLKAKLEAEGLFDPALKRPLPRFPKRIAVATSPTGAAVRDII
                     TVARRRWPAIHIVIIPTLVQGEGAPASIVCSIEHANALAEAGGADGIDVLIVGRGGGS
                     AEELWAFNDEMVARAIRSSRVPVVSAVGHETDFTIADFAADVRAATPSAAAELVVPDI
                     GVYLRQLETERTRLVRSVRRLIDQRRQRVDELRRWHVQKTQTRFRASRERVQRLAGVL
                     SALDPQAVLGRGYAICRGPDGAVLKDSQSVRTGDTVSVKLGRGAIGCEVREVVQG"
     gene            complement(2115..>2836)
                     /locus_tag="PLM74_10750"
     CDS             complement(2115..>2836)
                     /locus_tag="PLM74_10750"
                     /inference="COORDINATES: protein motif:HMM:NF013012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-sialoglycoprotein endopeptidase"
                     /protein_id="HON43588.1"
                     /translation="GSYMPVFLAGSGLARAVSAALGAPYIEVSHQEGHIAAASWSSEL
                     GIARRGGFLALHLSGGTTELLAVHDRTNVELLGGTEDLNAGQFIDRVGVRLGIPFPAG
                     PGMEQLAAGGEPGAVRLPVAVARGREIGRPAVSFSGPCSAAMRELDAGKRPADVALGI
                     LECIGRSVAALCVDGLRATGLSDVLASGGVASNSIIRGIMQHEVVGAVPLSSLYFADP
                     EHSRDNAVGVALLAWQAHIAD"
BASE COUNT          557 a         1030 c          774 g          475 t
ORIGIN      
        1 agtccgctcg cgccacccac gatgcccccc atccgtgccg cggcctcgaa caggcgcccc
       61 gtcttcatcg attccatccg ctgcacagct tctgccgcct cgcacagcgg ccctgaccat
      121 gattgctggg atatggcaag ctctagtgcc tgccccccag ccatgcccag gcttccggac
      181 gcttgggcaa gctcgctcgc caacaggcat gccgagtggg ctccggccgc ctccacgaac
      241 tcgggtagcg caagcgactc aaacgccagc gtaagcagag catcgcctgc aagaagcgcc
      301 accgcctcgc cgtacgtccg atggcatgac gggcgacctc gtctgaagtc atcgtcatcc
      361 atgcatggaa ggtcgtcgtg gatcagtgag tagcagtgta tcaactccac cgcagcaccc
      421 gcgcgcaccg ccagcgcacg gcggccatca ggcgaaacac ccggcgcacc tcgctccgtg
      481 tgcaccgcct caaaggccgc cacagccagc gcgggacgga gcctcttgcc gccggggaat
      541 accgagtatc tcattgccgc atgcagagcc tgcggcggtg tgccgccgct cggcatcaat
      601 gcatcgagcg cctcgttgac aacctgcgac acttgggcga gccacgcaac aggcgcagct
      661 ttgaaactag agttgctcaa tcgccgttct ccccctctag cttgcccagc attccctggg
      721 caggcgccac ttccggcttg ccgtcttttc cctcgatgag catctccagt cggccctccg
      781 cctgatccag aatggcggaa cagaacttgc ccagttcgct gccttcgcca aatagctcga
      841 gggcctcgtc aagcggcaag tcgcccgcgt cgagctgcgc gactatctgc tccagacgag
      901 ccagcgcctt ctcaaaagtc agctctgcct tcatccctga accacctccc gaacctcaca
      961 gccaatggcc ccacggccca gtttcacgct gaccgtatct cccgtgcgca cgctttgcga
     1021 atccttcagc actgcaccgt ccggaccccg gcatatagca tatccacggc ccaggacggc
     1081 ctggggatca agggccgata acactcctgc gaggcgctgc acgcgctccc gggaggcccg
     1141 gaacctcgtc tgagtcttct gaacatgcca ccgccgcagc tcatctacgc gctgccgacg
     1201 ctggtcgatc aggcgccgca ccgacctcac aagcctcgtg cgctccgtct ccagctgccg
     1261 aagatagacc ccaatgtcgg gcaccacgag ctcggcggct gctgacgggg ttgcagcacg
     1321 aacatcggca gcgaagtcgg cgatggtgaa atcggtctcg tgccccactg ccgacaccac
     1381 cggaacgcgc gatgatctga tcgctctggc caccatctcg tcgttgaagg cccagagctc
     1441 ctcagcggac ccgccccccc gccctacgat gagcacgtcg atgccatcgg caccgcccgc
     1501 ctcagcgagc gcgttggcat gttcaatgga gcacacgatg ctggcgggcg ccccttcgcc
     1561 ctgcaccagt gtgggaatga tgacgatatg tatggcaggc cagcgccgtc gggcaacggt
     1621 gatgatgtcg cggaccgccg caccggtcgg agatgtcgcc accgcgatac gcttcgggaa
     1681 tcggggcaga ggccgcttaa gtgccgggtc gaatagtcct tccgcctcca gcttggcttt
     1741 gagctgctga aaggcgagga ataaccctcc gaggcctgca ggttccatga agtctatgta
     1801 aagctggtac tcgccaccgc ttctgtacac acccacgtac ccgtgagcga acacccgcat
     1861 gccgctctcc ggacgaaaac gaagccctct attggccccc tggaacatga ccgcgcgcag
     1921 acgagaggcg tcgtccttaa gcgtcatgta catatgcccg gacgaatggt gcgtgaagtt
     1981 ggatatctcg ccgcttaccc acacatcggc aaggactgcg tccgcctgaa gcagcctcgt
     2041 caccctatca gtgatctggg agactgaata gacctcaatc tccagaccgt acgcgttcac
     2101 acgatgcccc cccatcaatc ggcaatatgc gcctgccacg ccagaagcgc aacgcccaca
     2161 gcattgtcgc gcgaatgctc gggatctgcg aaatacagcg atgaaagcgg aaccgcgccc
     2221 accacttcgt gctgcattat cccacgtatg atcgaattcg aagccactcc gcccgaggcc
     2281 agcacgtcac ttaggcccgt agcccgcagc ccatccacac agagcgccgc gactgatcga
     2341 ccaatgcact ccaaaatgcc aagagccaca tccgctggac gcttcccggc atctagctcg
     2401 cgcatggcag ctgaacatgg cccggagaag ctaacggccg ggcgccctat ctcacgcccc
     2461 cgcgccacgg ccaccggtaa cctcactgcc ccaggctctc cgcccgccgc caattgctcc
     2521 atgcccggac ccgccggaaa gggtatccca agcctcacgc cgactctgtc gatgaactgg
     2581 cccgcgttca gatcttcagt acctcccagc agctcaacgt tggtcctatc gtgcaccgcg
     2641 agaagctcgg ttgtgcctcc cgaaaggtgg agcgccaaaa agccgccgcg ccgcgcgatc
     2701 cccagctccg atgaccagct ggcggctgcg atgtgtccct cttgatgcga tacctcgatg
     2761 tagggcgctc ccagcgccgc agatactgcg cgggcaagac ccgagccggc caggaagaca
     2821 ggcatgtatg accccc
//
LOCUS       DAOTAC010000306         2830 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_27641_length_2830_cov_3.856216, whole genome shotgun sequence.
ACCESSION   DAOTAC010000306 DAOTAC010000000
VERSION     DAOTAC010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27641_length_2830_cov_3.856216"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="PLM74_10755"
     CDS             complement(<1..288)
                     /locus_tag="PLM74_10755"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007953417.1"
                     /note="catalyzes a two-step reaction, first charging a
                     tryptophan molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HON43589.1"
                     /translation="MKQGRIFSGMRPTGMLHLGNLAGALNNWVRLQDDYECLFAAVDW
                     HALTTGYQDTSMIREYTRQVLIDFIAAGIDPERSIVFRQSDVKEHAELHLLL"
     gene            complement(301..918)
                     /locus_tag="PLM74_10760"
     CDS             complement(301..918)
                     /locus_tag="PLM74_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HON43590.1"
                     /translation="MSGNDILYFLTTLPILLLALSVHEYAHAWAAVRLGDGTPRWDGR
                     LTLNPLAHLDPIGLLALVLTRRFGWAKPVRVNPMNLANREAGMVMVSLAGPGANVVLA
                     ILLSAMIKLIYGAYGFRREATALLVMALDQGVWINLGLAVFNLIPIPPLDGSKILAAV
                     LSGDAARVYYRLEQYSYIILLVFIMTPLSTWILVPIVRALYRAIM"
     gene            complement(961..2085)
                     /locus_tag="PLM74_10765"
     CDS             complement(961..2085)
                     /locus_tag="PLM74_10765"
                     /inference="COORDINATES: protein motif:HMM:NF021504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HON43591.1"
                     /translation="MRITFPHLGNLWIPVKAFLSSLGHDVITPPPTTKRTLSIGARHT
                     PDGACLPLKVTMGNFIEAAELGADAILMVGGIGPCRLGYYCELQRRALARAGCSAQLI
                     VVEPPRSGLLEFANVVRTIRRRATLGQILEASRLAWAKFGAVDALDQAANAARWREAR
                     PGSVDACMRRGLALIDTAETCDAVRGFVEQTLHDVEGCVEAKPRPAVRVGVVGEIYIV
                     MEPAVNMDVCKRLGRLGAQVVQPLTFSHWIESHVVCDIARWIPKPDVLSWSRPYLRHF
                     VGGHGVESVGHSVEMARSGLHGVVHLAPMTCMPEIVAQSVLPCVSLAEGIPVMSLAVD
                     EHTADVGFDTRLEAFFDLLSARVCEEGGSSSCPGSCPVST"
     gene            complement(2039..>2830)
                     /locus_tag="PLM74_10770"
     CDS             complement(2039..>2830)
                     /locus_tag="PLM74_10770"
                     /inference="COORDINATES: protein motif:HMM:NF021504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HON43592.1"
                     /translation="LAEHVDALFVPRFVSVERGAYICPKLMGLPDMVRARAPGVPRLV
                     DPCIDMSRGLGKMARSMVELGPALGVSSSRLAIALARSLKAASATAKTRLGGEQTAVT
                     YGGVRIGVLGHEYNLNDPYISLDLVGKIRRLGATPVTAGSYSHQETVRWERQRIRRGA
                     KRLFWTYGRRLLGAAYRWLDTGEVDGIVHLASFGCGPESFISEIIQYEAGEQNSLPLL
                     CVNIDEHSAEAGLSTRLEAFIDTVAMRKCEYANHLPTPGQPVDTR"
BASE COUNT          602 a          899 c          772 g          557 t
ORIGIN      
        1 aagcaacaag tgcagttcag catgttcctt cacatcagac tgacggaaga cgatggagcg
       61 ctctgggtcg atgcccgccg cgataaagtc gatgagcact tgcctggtgt actcgcggat
      121 catcgatgtg tcctggtatc ctgtggtgag agcatgccag tcgacggctg cgaacaaaca
      181 ctcatagtca tcctgcaacc gaacccagtt gttcagcgct cctgccaggt tgcccaggtg
      241 aagcattcct gtgggcctca tcccgctgaa tatccggcct tgtttcaacc aaaccaccct
      301 ctacataatt gctcgatata gcgccctgac gattggaacg agaatccatg tcgatagcgg
      361 cgtcatgatg aacaccagga gaatgatgta cgagtactgc tccagtcgat agtagaccct
      421 tgccgcgtcg ccggagagca cggcagccag tatcttcgag ccgtccagcg gcggaatggg
      481 tatcaagttg aaaacagcta accccaggtt tatccatacc ccctggtcga gggccatgac
      541 gagaagggcg gttgcctcac gccgaaaccc atatgcgcca tatatgagct tgatcatggc
      601 cgacagaagt atggcgagga ccacgttggc ccctgggccg gccaggctaa ccatgaccat
      661 tcccgcttcg cggttagcaa ggttcatagg gttgaccctt acaggtttcg cccagccaaa
      721 ccgcctggta agcaccagag ccagaaggcc tatggggtcg agatgggcca ggggattgag
      781 cgtgaggcgc ccgtcccacc tgggagtgcc gtctcccagt ctcaccgcgg cccaggcatg
      841 cgcgtactca tgcaccgaca gtgcaagcag gaggattggc agggtagtta ggaaatacag
      901 aatatcattg ccactcaata gcgccacctc ccctgcgctc gattatagca tagaccgacg
      961 tcaggtggaa accgggcacg accccgggca tgacgacgac ccgccttcct cgcacactct
     1021 ggccgacaac agatcgaaaa aggcttcgag cctagtatcg aaaccaacat cagccgtgtg
     1081 ttcgtccaca gctagcgaca tcactgggat gccctcagcc aggctgacac atggaaggac
     1141 gctttgggcc acgatctccg gcatacatgt cataggtgcc aggtgcacta ccccatgcag
     1201 gcctgaccgt gccatttcca ctgagtgccc tacgctttca accccgtgcc ctcccacgaa
     1261 atgccgcagg tagggtcgag accaagatag gacatcgggt ttcgggatcc accgggctat
     1321 gtcgcacact acgtgcgatt cgatccagtg tgagaacgta agcggctgta ctacctgtgc
     1381 gcccagtcgg cccagtctct tgcatacgtc catgttcacc gccggttcca tcacgatgta
     1441 gatctcgccc acgacaccca cgcgtactgc aggccgcggc ttggcctcga cgcagccttc
     1501 cacatcatga agcgtctgct ccacaaatcc ccttacggca tcgcatgttt cagccgtgtc
     1561 gatgagcgct aacccacgcc tcatacatgc gtctacactc ccaggcctgg cttcgcgcca
     1621 tcgcgccgca ttagccgcct ggtcgagcgc gtcgaccgcc ccaaacttgg cccaggcaag
     1681 ccggctagct tctagtatct gcccaagcgt ggcacggcgt ctgattgttc tgaccacgtt
     1741 cgcaaactcc agcagacccg accgcggagg ttccaccacg atcagctgag cgctgcatcc
     1801 cgctctggcc agcgctcgtc gctgaagctc gcaatagtac cccagcctgc acggcccgat
     1861 ccccccaacc atgaggatag cgtcggcacc cagttcagcc gcctcgatga agttgcccat
     1921 ggtcaccttc aacggaaggc atgcgccgtc aggtgtgtgc ctcgccccta ttgagagtgt
     1981 tcttttcgtg gtgggaggtg gagtgatcac atcatggcca agtgaagaca ggaacgcctt
     2041 aacgggtatc cacaggttgc ccaggtgtgg gaaggtgatt cgcatattcg cacttcctca
     2101 tagctaccgt gtcgataaat gcttccaggc gcgtggagag accggcctcg gctgagtgct
     2161 cgtcgatatt cacacacaac aggggcagag agttctgctc acccgcctca tactggatga
     2221 tttcggagat gaacgattcc ggcccgcaac cgaaactcgc gaggtgcacg atcccgtcca
     2281 cctcgcctgt gtcaagccat cggtacgccg ccccaagcag gcgacgaccg tacgtccaga
     2341 agaggcgttt ggccccacgc cgaatccgtt ggcgctccca tcggaccgtc tcttggtgag
     2401 aataggagcc ggctgtcaca ggtgtagccc ccaaacgccg gattttgccg accaggtcca
     2461 ggctgatgta cggatcgttg aggttgtact catgccccag aacccctatg cgtactccgc
     2521 cataggttac ggccgtttgc tcgccgccga gccgcgtttt ggcagtcgca gatgctgctt
     2581 tcagggatct tgccaacgca atcgccaggc gactgctgct tacgccgagc gccggtccga
     2641 gctcgaccat ggatcttgcc atcttcccaa ggcctcgcga catatcgatg cacggatcga
     2701 ctagcctcgg cacaccgggc gccctggcgc gtaccatgtc gggcaacccc atcagcttag
     2761 ggcagatgta ggcgcctcgc tcaacgctga cgaatctcgg aacgaacaag gcatctacgt
     2821 gttctgctag
//
LOCUS       DAOTAC010000307         2826 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_27702_length_2826_cov_3.666185, whole genome shotgun sequence.
ACCESSION   DAOTAC010000307 DAOTAC010000000
VERSION     DAOTAC010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27702_length_2826_cov_3.666185"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            948..2276
                     /locus_tag="PLM74_10775"
     CDS             948..2276
                     /locus_tag="PLM74_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43593.1"
                     /translation="MKKLAVVLAAVMVAALALPVSAATIDLGGKLSSEVYHDFVKGAG
                     GSTALELTAGIGLIGKQNVKGVVKVSGMKWSLDLTEDGGIGLPDPTKVGITAYLETVG
                     PYWTGGPSVATRIGDLELAYSPFILETKAVDGISVSGAAVGPVAIDGFYGWVGGAAVR
                     GARAKTEFAGVGIDGAFARVGDLETDYMAHAAAVPMPGLAIEALYAAQSNTNAAAMRA
                     AAAYRIPFLPLDVTASAGYRRTGTEFAPTFRSTEKGNPLEGQAGVAAITAGLDTSIAG
                     FDIAVDGEILGNGNDGKMDDQRSIGAAVGTKVGWIDVAAGHKVTQKDLTTQNPSTTNT
                     TTLGVGFAERPILPNLVMSASYNATITNFSLSGMRHVAKAAMGTDIGAFRGVSVSGMI
                     DTQPAEGDPGRQLGVEYGAPCGVKLGYSYEDYASNEKDANRVYAGLEVSF"
     gene            complement(2395..>2826)
                     /locus_tag="PLM74_10780"
     CDS             complement(2395..>2826)
                     /locus_tag="PLM74_10780"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HON43594.1"
                     /translation="VLAGCRSARINVRGSGAVVADPRPVGSFTRVTFAIPATLIVQPG
                     DTLFLIARRYGTTVQALMSANNLTSTNIYVGQRLRIPGGPRGGPRTESVGQAGSGEYV
                     VQPGDTLSGIASAFGATQEALVGLNQLLSSDIYPGQNLRIP"
BASE COUNT          608 a          822 c          892 g          504 t
ORIGIN      
        1 tccggggcgc ctcggccttt gtatccgggc cgatccatgg atatgggtaa cgtgtcgcat
       61 attgcttaca ggttcgcgaa ctccctacca aacacggttt tgcccaaagg cctgcgcaac
      121 gtcgccttca ccaacaactt gcatctccga gcgtaggcag gtgcccggcg caaggtgttc
      181 ggccaatcag caagactcgc atcctttcca gcgatgtggg tagtgcaaga cttctgcagc
      241 tctctgtgcg acacggtatg cagcggtaac acttcgctca agaggcccta cacggcacac
      301 gagtcgcgaa atggcgctgg gctgggattt gcacgaacgt atagaaaacc cacaggggat
      361 ggggcacgtt ggaaatacac gtgggagtgg taggcgtggt ccacccttgt cagacgaact
      421 gcagaagtcc tggcagtgca tagcgccatg gagacgggtt cgtttcactg tttggcaggg
      481 agttagcgag tctgcttcgg ggcgctttca agggcgctta tggaattcat gatgcctgac
      541 ccctacaaat ccacaactta caccctgcta aagacccagt tacctgcagg aacgtgccgc
      601 gccaacctag aattcatacc ccacaacagg cgatgctgga agcatcagtc ctgcagtaaa
      661 ccggagcatg atgccagagg cgtgtcgaga ggcgggaaag gaggtgctcc cccaaaagcc
      721 caggagtgca cgtccgcggc gcaacacatc gcagcagaac cgcagagtct tgcttgatgg
      781 caacaaggac acttcaggaa gcccctgcgg caagacgcag cacaaatcgc ggcacttgtc
      841 ggaccattcg cctggcgcaa tgttccacga cagagcaaga atcgtcagac tgctccggag
      901 acgtagcaat atgcgaagtc attagtagct accaaggggg agaacacatg aagaagcttg
      961 cagtagtact tgcagcagta atggttgcag cgcttgcact gcctgtttcg gccgctacga
     1021 tcgatctcgg tggcaagctg tcgagcgagg tctaccacga cttcgtgaag ggtgccggcg
     1081 gcagcaccgc tctcgaactg acggccggta tagggctcat cggaaagcag aatgtcaagg
     1141 gcgtcgtgaa ggtgagcggg atgaagtggt cgctcgacct gaccgaagat ggcggaatcg
     1201 gtctgccgga tccgaccaag gtcggcatca ccgcttacct tgagaccgtc gggccatact
     1261 ggaccggcgg accgtcggtg gcgacccgca taggcgatct cgaactagcc tattcgccgt
     1321 tcatactcga gacaaaggcc gttgacggaa tctcagtgtc gggagcagcc gttggacctg
     1381 tagcaatcga tggtttctac ggctgggtag gcggagcggc cgtgcgaggc gcgcgcgcca
     1441 agactgagtt tgcgggtgtc ggcatagacg gggcattcgc aagggtcggg gatttggaga
     1501 ctgactacat ggctcacgcc gctgcagtgc ccatgccggg cctggccatt gaggctctat
     1561 atgcggcaca aagcaatacc aacgctgcag cgatgcgcgc tgcggcagcc tacaggatcc
     1621 cgttcctgcc tctggacgta acggccagtg caggctacag aaggaccggg acggaattcg
     1681 cgccgacttt ccggtcgacc gagaagggca acccactcga ggggcaggca ggcgtagcgg
     1741 ctatcaccgc cggccttgat acctcgatcg caggattcga catcgccgtc gatggcgaaa
     1801 tcctcggcaa cggcaacgat ggcaagatgg acgaccagcg ctccatcggc gcggctgtgg
     1861 gcactaaggt tggatggatc gacgtggcag cgggccacaa ggttactcag aaggacctga
     1921 cgacgcagaa cccctctacc accaacacca cgactctggg agttgggttc gcggagcgtc
     1981 cgatcctgcc gaacctggta atgagcgcga gctacaatgc gacgatcacc aacttctcgc
     2041 tgtccgggat gaggcacgtg gccaaggccg ccatgggtac ggatatcgga gcattccgtg
     2101 gagtcagcgt atccggcatg atcgacacac agcccgcaga gggagacccg ggacgccagc
     2161 tcggcgtgga atacggtgcg ccgtgcggag tcaagctcgg atactcctac gaggactacg
     2221 ccagcaacga gaaggacgcc aacagggtct acgcaggtct ggaggtatcc ttctagtcaa
     2281 gaccgtcaat gcgcggtcgg atcgcgaccg cggcccaaag cgcaaggacc cgggatatcc
     2341 cgggtccttg cacgtccgac gcaagtttct cgcatagagg cctgccttcc ctgcctacgg
     2401 gattctcaga ttctgcccag ggtagatatc gctgctgagc agctggttta accccactag
     2461 cgcctcctgc gtcgcgccga atgccgaggc tatgccggac agcgtatcgc cgggctgtac
     2521 cacgtactcg ccggaccctg cctgcccaac cgattccgtt ctggggcctc ccctcggccc
     2581 gcccggtatc ctgagtcgct gacccacata gatgttggtg ctggtcaggt tgtttgcgga
     2641 catcagagcc tgcactgtcg tgccgtacct ccgcgctatc aggaataggg tgtcgccggg
     2701 ctggacgatg agggtggcgg gaatagcgaa ggtgacgcgg gtgaagctac caacggggcg
     2761 ggggtcggcg acgacggcgc cggagccgcg cacgttgata cgggcggagc ggcagccagc
     2821 caggac
//
LOCUS       DAOTAC010000308         2769 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_28403_length_2769_cov_4.705601, whole genome shotgun sequence.
ACCESSION   DAOTAC010000308 DAOTAC010000000
VERSION     DAOTAC010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28403_length_2769_cov_4.705601"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(272..1438)
                     /locus_tag="PLM74_10785"
     CDS             complement(272..1438)
                     /locus_tag="PLM74_10785"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HON43595.1"
                     /translation="MASTRTEVYRLIRDEGPISRSGIQSKLDISAASVSTVVSGLLEQ
                     ELVVRGRIVGNGVGRKTELLEHNPRKGYVLGVDLGAKAIRVAVADLCGAIVHKTETST
                     LAYEGGARVMQRLMEACRSAVASADIHKSKILAIGVAAPGISLGKTGRSILAPFIPDW
                     SDVPFRSLLQSEFGCEVLVENDVDMAIIGERRWGVGKGCDNLVFINMAVGVAAGIIVD
                     GNLIKGCRNGAGEIGYMVADPSMIRPAFSVQGALERSISGPGIARRWRDLVGDADRAG
                     DEPAQIVDSEMVLALAAQGDVNARKVIDDTLQYLCSALSNIAAVVNPEKVILGGGVGL
                     ALCSYARSIAEFLKHHLPFPPEIVQAELGSDASLFGSIAVAIDSAFDALERGIG"
     gene            1988..2557
                     /locus_tag="PLM74_10790"
     CDS             1988..2557
                     /locus_tag="PLM74_10790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HON43596.1"
                     /translation="MHQKARPASEDALVSRLRAGHEAAADELVDLYADRLLRAASLLL
                     NDRHLAEDAVQETLLATVTRIGQFRGESSLYSWMYAILMRWCRRQQHRRKIDSNLSLL
                     SSEELTDVAGEDVSVEVAWEKAQRTLQVSRAVGGLPQAYREVVVLFYHEEFSIAEISR
                     MTGKPEGTIKSLLHRARQKLAQTLEGDEL"
     gene            2554..>2769
                     /locus_tag="PLM74_10795"
     CDS             2554..>2769
                     /locus_tag="PLM74_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43597.1"
                     /translation="MIDNELKRTLQDEMRRVSFSGDEKQRLAAQLKDEMRTGKAAPAP
                     ARRWEAFVEWVAAFWNGTTEISLPAATA"
BASE COUNT          595 a          866 c          750 g          558 t
ORIGIN      
        1 atccgagcag gcatgccgtc agtttttgcc gatagttttg ccgataggcg caattgccgg
       61 tatctcaaaa actattgccg aaaactgacg atataccagc tccagccgtt ttgacccacc
      121 accatgatag ttataccagc caaaacgcca tttcggcgag gatgcccgct tccaccgtca
      181 ctccgtctgg caattcctgc tcgcattcac acttcgcggt gcctacaaga actatacaga
      241 tcccaacccc tgcgcgaccg ccgtgcgacc cctacccgat cccacgctcc agggcgtcaa
      301 atgcggagtc aatcgctact gcaatcgaac cgaaaagact ggcatcgctt ccgagctcgg
      361 cctggacgat ctccggcgga aacggaagat gatgcttcag gaactccgct atgctccgtg
      421 cgtagctgca tagtgccagg cccaccccgc cgcccaggat gaccttctcc gggttgacga
      481 ccgccgcgat gttgctcagc gctgaacata agtactgcaa agtgtcgtcg atcactttcc
      541 gcgcgttcac atcgccctgt gcggcgaggg ccaaaaccat ttcggagtcc acaatctgcg
      601 ccggctcgtc gcctgctcgg tcggcgtcgc ctaccaggtc tctccacctc ctggctattc
      661 caggccccga gatggaccgc tccagtgcgc cctggacgct gaatgcaggc cttatcatgg
      721 acggatctgc aaccatgtac ccgatctcgc ccgccccatt gcggcaaccc ttaatgaggt
      781 tgccgtcgac gattattccg gcagctacgc ccactgccat gttgatgaag accagattgt
      841 cgcagccctt tcccactccc cagcgccttt ctccgattat cgccatgtca acatcgtttt
      901 ccacaagcac ctcgcaaccg aactccgact gaagcaaaga ccgaaacggc acgtcagacc
      961 aatccgggat gaacggcgcc aaaatgctcc gccctgtctt gcccaggctg atgcctggcg
     1021 cggctactcc gatggcaagg atcttcgact tgtgtatgtc tgccgatgca acagcgcttc
     1081 tgcacgcttc cataaggcgc tgcatgactc gagcgccgcc ttcataggcc agcgtagacg
     1141 tctcagtctt gtgaacgata gctccacaca gatcggccac cgcaaccctg atcgccttag
     1201 cgcccaggtc cacaccgagc acatatccct tccgcggatt gtgctcgaga agctcagtct
     1261 tgcgcccgac tccgtttccg acaatccggc ccctgactac gagttcctgt tcgagcaacc
     1321 cggacacaac cgtcgaaaca gaagccgcac tgatatcgag cttcgactgg atcccgctgc
     1381 gtgaaatcgg cccctcgtcg cggatgagac ggtagacctc cgttcttgtg gaagccatcg
     1441 ttaaccaacc tccaggctaa gtcttcaagc tcagtcatca gccttagccg tcgaacgcgg
     1501 ccgtcggcct tagccgtggg gcgtggccgt caagctcagc gcccagcttg aattccgtca
     1561 ggccaagaaa ggcttttggc ctattgcatc actgcgaaat atatgcgttc gttgcacggt
     1621 ctcatgacgt ttctttcact ccgctgaact attcgatgcc attgagcaga atcctgctct
     1681 ccattgtgta tcaatgaaat gtcgccccgc ggccagcgag aggcatcaca catgcgatga
     1741 caggagtcac tcatcttaag aggcatcacc catccgacga caggagttgc tcatcccaag
     1801 tggaagcaac atccgcagga tgcgcagccc cgccaacact ttcagctgtt ctcgcgactg
     1861 ggttctcgcg attgggttct cgcgattggg ttcgagatgc gatgcaacct tttctccccc
     1921 gaccgcctct taaggatcgg aagggcgggg actggtgttg acggctgcca aggagttgtc
     1981 tatcgggatg catcaaaaag caagaccggc atcggaggat gcgctggtca gccggctgcg
     2041 cgccgggcac gaagccgcag ccgatgaatt ggtggacctt tacgcagacc ggctgttgcg
     2101 ggctgcgtcc cttctgctga acgataggca tctggcagaa gacgcagtgc aggagacgct
     2161 gcttgccacg gtaacccgca tcgggcagtt tcgcggagag tcgtcactgt actcatggat
     2221 gtatgccatc ctgatgagat ggtgccgccg gcagcagcat cgccgcaaga tcgatagcaa
     2281 cctgagcctg ctgtcgtctg aagaactgac cgacgttgcc ggtgaggatg tctcggttga
     2341 ggtcgcgtgg gagaaggcac agcgaaccct ccaggtaagc cgcgcagtag gcggccttcc
     2401 acaagcctat cgcgaagtgg tggtgctgtt ctaccacgag gagttctcca tcgccgagat
     2461 atctcgcatg accggcaaac ccgagggcac gatcaagagt ctcttacata gagccaggca
     2521 gaaactagcg cagacccttg agggggatga actatgatcg ataatgagtt gaaaaggaca
     2581 ttgcaggatg agatgagacg ggtgagcttc tccggggacg aaaagcagcg tcttgctgcg
     2641 caactcaagg acgagatgcg cacaggcaag gccgcgccgg cgcccgcgag gcgctgggag
     2701 gcattcgtcg aatgggtagc ggccttctgg aacggaacca cggagatctc gctccccgca
     2761 gctacggca
//
LOCUS       DAOTAC010000309         2714 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_29103_length_2714_cov_4.341858, whole genome shotgun sequence.
ACCESSION   DAOTAC010000309 DAOTAC010000000
VERSION     DAOTAC010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29103_length_2714_cov_4.341858"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            591..1490
                     /locus_tag="PLM74_10800"
     CDS             591..1490
                     /locus_tag="PLM74_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HON43598.1"
                     /translation="MIISASRRTDIPAFYSQWFMNRIRQGSCVVVNPFNSSQRHYVDL
                     TPDAVDAIVFWSKNPAPLLPHLGELDDMGYRYYFQLTVCNYPRDIEQYLPPLPERMAI
                     AVELARRIGPRRVIWRYDPIIVSNRTDAQFHRRQFAAIASALEGSTTRVVVSLMDFYG
                     KVERNLLGVLESQAWQFVRTAGVDEHTRELLSNLRNESLAHGLEIQSCAEAVDMSDLG
                     ISHGKCIDGDLIRSIWGIEVDGRKDPGQRSACGCAPSRDIGANNTCIHGCRYCYATIS
                     HRAALNRYANHDPSSPDLAGRAR"
     gene            complement(1680..2336)
                     /locus_tag="PLM74_10805"
     CDS             complement(1680..2336)
                     /locus_tag="PLM74_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013932409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON43599.1"
                     /translation="MLQVKSLKAGYDNVPVVFGVDFEVGQGEIVAIVGSNGAGKTTIV
                     RAVSGLIRPMEGTVEFVGRPVQGMRAHEITSLGVAHVPEGRRVFAKMSVEDNLLLGAH
                     TVREQSAVAQTLQEVYEVFPVLHSRRDQKAETLSGGEQQMLAVGRGLMPTMVDRVLQV
                     LREIRDRGVTVLMVEQKVEKALAMADRAYVLQTGRIAMSGIGRELLENPEIKRAYLGL
                     "
     gene            complement(2323..>2714)
                     /locus_tag="PLM74_10810"
     CDS             complement(2323..>2714)
                     /locus_tag="PLM74_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013932408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON43600.1"
                     /translation="RLGLVRTFQIVHTFRDMTVLENVVTSAFLRAGNSKDALATAPRL
                     IMLDETIAGLNQTEIREAMDLCLRLKKDGLTLVVVEHIMEALMPISDRVVVLDSGRKI
                     MEGTPEEVASDERVIKAYLGDTYNVAG"
BASE COUNT          577 a          836 c          733 g          568 t
ORIGIN      
        1 gcctgcttcg ctgccccaac ccgtcggata ccccccgaat gtcgcgattt ggaggcggtc
       61 aggtggccct tcgaaatcga tgtactcaaa aattgccggt atctccaaaa ctgatggcaa
      121 aaatcgacga tatgccagcc ccagccgttt tgtctgatgg ccatgatgtc aagaccagct
      181 aaacgtccat tccagcgagg cgagcatgac ccacgcagcc aacaacccgc cgtgatggtc
      241 gaggatgatg tcatttttcc gctcactatc ccgttattga gcaaaggctc gccgcgctgg
      301 catgcattcc gccgctacag tgaatctgcc tcccttcgaa gccctcaagg cctattccct
      361 actcaggcag agagaaagac atccgggtgc agctagatgc gatatcaacc gtgaaccggg
      421 acagtcgata gcgaattggc gagaggtcaa ccgcgtcgcc gtggcagcat atgggatccc
      481 gcccaccgcc caccgcccat acgccgatag ctcagcgccg gccaagggat cgtctccttg
      541 aaggagagtg tgacttcgac agcgaattcg aggccgaacg gaggcgccac atgatcatct
      601 cggcaagtag acgcactgac attcccgctt tctacagcca gtggttcatg aacagaatcc
      661 ggcagggatc ctgtgttgtg gtcaacccgt tcaactcttc gcagagacac tatgttgatc
      721 tgaccccgga tgcagtcgac gcgatagtgt tttggagcaa gaaccctgcg ccgctccttc
      781 ctcatctggg agagttggat gatatgggct accgctacta cttccagctc acggtctgca
      841 actacccgcg agatatcgag cagtacctgc cgccgctgcc cgaacgcatg gcgatcgcgg
      901 tggagctagc ccgcaggata ggtccgcgca gggtcatatg gaggtatgac cctatcatcg
      961 tctccaaccg cacagacgcg cagttccaca ggcgtcagtt cgctgcgata gcctcggcat
     1021 tggaaggctc gactactcga gtggtagtga gccttatgga cttctacgga aaggtcgagc
     1081 gaaacctgct cggagtgttg gaaagccagg catggcagtt tgttcgcacc gctggggttg
     1141 acgagcatac ccgcgaactc ctcagcaatc ttcgcaacga atctcttgcc cacggcctag
     1201 agatccagtc atgcgccgag gcggtcgaca tgtccgacct ggggatatcc catggcaagt
     1261 gcattgatgg cgatctgatc cgcagcatat ggggcattga ggtggacggc cgcaaagacc
     1321 ccggccagag aagcgcatgt ggctgcgcgc cgagccgaga catcggcgcc aacaacacgt
     1381 gcatccatgg gtgcaggtac tgctatgcaa cgattagcca cagagcagcg ctcaaccggt
     1441 acgctaacca tgacccaagc tcgccggacc tggctgggcg ggcgcgctga tggcagacgc
     1501 tgatgagcac tcaactatct acagaaaacg gaaagggaat tgccaagtac acaaggcccg
     1561 gctgaaagcg cttcaggaga gggaggactt tgagcagtgg gatccaggac tgcctgctcc
     1621 gcgctgccga agaacgcaga gcatcctccc cctcgccgag gcgccccccg cgatctaggc
     1681 tacaagccca gatacgcccg ctttatttcc gggttctcga gcagctcgcg gccaatgcca
     1741 ctcatggcga tcctgcctgt ttggagcaca taggctctgt ctgccatggc gagggccttt
     1801 tccaccttct gctctaccat cagcactgtc acgccgcggt ctcggatctc gcgcagaacc
     1861 tgcagcactc ggtctaccat agtcggcatg agtccccgtc ccacggcgag catttgctgt
     1921 tcgccgcctg ataacgtctc cgctttctgg tcgcgcctgc tgtggagtac tggaaagact
     1981 tcatatacct cttgcagagt ttgggctacc gcagactgct ctcgtactgt gtgtgctcca
     2041 agaaggaggt tgtcttcgac actcattttt gcaaacacgc gccgtccttc cgggacgtga
     2101 gctactccta aggacgtgat ctcgtgcgcc cgcattccct gcacgggtct gccgacgaat
     2161 tccaccgtgc cttccatggg acggatcagg cccgacacag ctcggacgat ggtggtcttg
     2221 ccggcaccgt ttgacccaac aatggccaca atctctccct gcccgacttc gaaatccact
     2281 ccgaatacga ccggcacatt atcgtaaccc gccttaagcg acttaacctg caacattgta
     2341 ggtgtccccc agatacgctt ttatcactct ttcgtccgat gcgacttctt cgggcgtccc
     2401 ttccatgatc ttacgacccg aatcgagcac tacgactcgg tcggatatgg gcatcagcgc
     2461 ttccatgatg tgctccacga ccacaagagt caggccgtcc ttcttgagac gcaggcaaag
     2521 gtccattgcc tccctgattt cagtctggtt cagccctgct attgtctcat ccagcatgat
     2581 gaggcgcggt gccgttgcca gggcatcttt cgagttccct gccctcagga atgcagatgt
     2641 aaccacgttc tcaaggacgg tcatgtctcg gaatgtatgt actatttgga acgttcggac
     2701 cagcccgaga cggg
//
LOCUS       DAOTAC010000310         2713 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_29117_length_2713_cov_6.049661, whole genome shotgun sequence.
ACCESSION   DAOTAC010000310 DAOTAC010000000
VERSION     DAOTAC010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29117_length_2713_cov_6.049661"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="PLM74_10815"
     CDS             <1..255
                     /locus_tag="PLM74_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43601.1"
                     /translation="YDVIKQALGMRKKPLALLSMLGLPFIIKYLIGRLTIEDVERKAK
                     TWLDIVGRGVIVPYAEIGVDVDKPSDYELACRVLPGSAGS"
     gene            327..707
                     /locus_tag="PLM74_10820"
     CDS             327..707
                     /locus_tag="PLM74_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HON43602.1"
                     /translation="MKSVVCTDAAPAAVGPYSQGIRVGNLLLVSGQLPLDPATGAFVE
                     GGIEEQTARCLENLKAILAQAGIGMDSVVKTTVFLADMDDFAAMNKVYASYFKTDPPA
                     RSAFQVARLPKNGQVEIEAIAWVG"
     gene            complement(910..984)
                     /locus_tag="PLM74_10825"
     tRNA            complement(910..984)
                     /locus_tag="PLM74_10825"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(950..952),aa:Gln,seq:ttg)
     gene            complement(2193..2402)
                     /locus_tag="PLM74_10830"
                     /pseudo
     CDS             complement(2193..2402)
                     /locus_tag="PLM74_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120941.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
BASE COUNT          542 a          762 c          810 g          599 t
ORIGIN      
        1 tacgacgtga tcaagcaggc tctgggcatg aggaagaagc ctcttgctct cctctcaatg
       61 ctgggcttgc cgttcattat caagtacctg atcgggcgcc ttaccataga ggatgtggag
      121 cgcaaggcga aaacatggct agacatcgtg ggtaggggag tgatcgttcc ctatgccgag
      181 atcggagtag atgtcgacaa acctagcgac tacgaattgg cctgccgcgt tctgccgggt
      241 agcgccggaa gctagagtag atgctgggaa ctaaaggcca agggccggag ctataggctg
      301 aaaccggagg ctgtggaggg atattcgtga agagtgtcgt ttgcaccgac gccgctcctg
      361 cggctgtcgg accttattct cagggtattc gcgtgggcaa tttgctgtta gtgtcggggc
      421 aactgccttt ggatccggcc acgggcgctt ttgtggaggg cggaattgag gagcagacag
      481 cacggtgctt ggagaatcta aaggcaatcc tggcacaggc gggcatcgga atggattcgg
      541 tggtgaagac cactgtgttt ctggcagaca tggacgactt tgcggcgatg aacaaggtat
      601 atgcctcgta cttcaagact gatccgcctg cacgttcagc cttccaggta gccaggcttc
      661 cgaagaacgg ccaagttgag atcgaggcca tcgcgtgggt cgggtaagta tcagcgagag
      721 acggcgatcg cggcctgcgg cagacgcacg aggtacatat gcggcaagca aagatactgg
      781 gccatcatat gcggccagcg aaaacatgag gccagtaaag gcctgtaaag gaaacggcag
      841 ggctacagcc ctgccggaca tttaacactt ccagcccttc cagttcgggt aacactcccg
      901 gttcggacat ggctggggcg ggaggattcg aacctccgaa tgcaggatcc aaagtcccgt
      961 gccttaccgc ttggctacgc cccatcgcag cgccgcgctt gcggcgcgtt tcaattctag
     1021 cataacgccg ggcagggcgc aattcctggc tcgttcaacc ataggcttga cgttctcctg
     1081 cggtgggccc taacggggtt ctggggcatc ctgttcgatt ctgaacagcg caggcccggc
     1141 ggaaaaccgg gcagaattct tctatgcatt acccccactc atgggacgag ctgagcgtgt
     1201 gtgacggctt gatcatcctg gacttctatc tgttgctgtt gaaatggaag tcaggtcatc
     1261 tggcggaaca gcgtgatgcg tggcctcgat gggaaaacag gcattgtgca gggatttgct
     1321 gagcctgagg ccgcttgctc gcggagtcgt ctgcatgcgg gcgcctttgg ctgagcctgc
     1381 agatattcgc actttccgcg gctcaacgcg gagatttttc gctgcatgtg tgcaggaaat
     1441 atccgcgacg ctggtttcgg accgcttggg cgccatagca cggaagacca cgcaccatca
     1501 taataccact aagctataag gaaagcctgc aacatacaga aatcccatat gtaagcacta
     1561 tggagcacct cacctgcctc aagatgccgc ttagtgccag cgttgtggca gaatcaggac
     1621 tccaggcgcg ctcacgcgga gatttttcgc cgcatgtgtg cagaaaatct ccgtccaacg
     1681 cctccagagg ggagaaaacc cgccaagata ccccttgggg gtataccgcc gcacccacga
     1741 ccccgatgcc tgactggact gacccatgga aacagggcag aacgcctcgc gctctgccct
     1801 gtgtgggctt gactgtgctc ttggatcgac tgtactccag gtttggccac gttcagcggg
     1861 tcaggctacg acttgcggta cgtcccgggc tagaccctgc cgggccctgc cgggccctgc
     1921 taggccctgc caggccccgc gtcatcaggt tggccagtcg cctagactgt accgcctggc
     1981 caattggggc cggggatgtt gcctcccacg taaccggtcg gctggcccga tggctgattc
     2041 aggggttcgt tcagtgactc gttcaggggt gcgtttgcgc ctactcccag gttggactgc
     2101 aatggattgg gttgtgcggc cccacctgtc ccggttgccc atggaccaac tgcctccgaa
     2161 aagccatgct tcactccggc cgcagttgtg gccgcgagcg atgcctgggc tgcctctatc
     2221 attctgcgga ccataccgcc gcccaccgac ccgcaatcgc gagaggtcat gtcgccccag
     2281 tatccaccct ggatattgtg cgtggggatg ttgagttcgc tagcgatctc ccacttcatc
     2341 tgttccaaag cctgccgcgc ttgcggcacc aacgtgcgct tgcttctgcg actcgctggc
     2401 atgccttcac ctccctcggg tttctgagta cattctttac acaggtcatc gggcatatgt
     2461 tagttaacgc gaggcgctca gaacgcttcc ttccacctgc gcgaaaagtt gacgaacctg
     2521 catgcttggt atatgctcaa agtgtttgtg gcttcatcaa cctagttcta tctgggtcta
     2581 gtcctatctg ggtctagtcc tatctgggtg gagtgaactt tatgatgtcc ttcgtggggc
     2641 ttgtactggc tgcagggctt ggcaagcgaa tgaagtcatc cttgcccaaa gcgcttcacc
     2701 aagttgccgg gca
//
LOCUS       DAOTAC010000311         2689 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_29424_length_2689_cov_4.676158, whole genome shotgun sequence.
ACCESSION   DAOTAC010000311 DAOTAC010000000
VERSION     DAOTAC010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29424_length_2689_cov_4.676158"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(260..>2689)
                     /locus_tag="PLM74_10835"
     CDS             complement(260..>2689)
                     /locus_tag="PLM74_10835"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HON43603.1"
                     /translation="HGDYMAMVTLGFGQIVRILLNNMDRPINITNGPNGIVAIDPPRI
                     LGFTLSSLESSYILIWVLALIILAGIARIIGSKTGRAWNALREDETAAACMGVNVRRY
                     RVSAFVTAAAIAGLAGALFASWQGAVFPQNFTMSETIALYCMAILGGIRSIPGIMLGV
                     LILQVLPEVLRAYSIYRMLIYGFALVLLAVFRPQGLIAQGEKLVGIGRRPLSDSRDSV
                     QSSAQSSAQGSTQETDAYIEADAGAGAGPKTDARLSSAAEAEPKSTVALGSVPVPVLE
                     ASGLSCNFGGLSAVSNVSFAAHAGEILGIIGPNGAGKTTLFNLITGLVKPSAGEVCVQ
                     GQTATGLDPHQVASLGVARTFQHIRLFETQSVLENVLAGCHLRGRSNVLGALARSVAH
                     TGQETEAVAQAREALALVDTSLLTREASLVRHLSYPDRRRVEVARAIATGAKLLLLDE
                     PAAGMTPEEITAMVDDIVALRDRGYTIVVIEHHMDLIAAACDRVIALDHGEVIAAGTP
                     QEVAAHPEVIRAYLGSAKTASAPTSTAAAADANAHADANADASADAATSGVAPILELA
                     GVHASYGPAEVLQGIDIEVRPGEVVALLGANAAGKSTAIKTIMGRLRPSSGEIRFAGR
                     RINGRPTNEIVRMGIGIVPEGRRVFYELTVLENLEVAAEAQAKAATAAMREAGSRARN
                     HAAAQSQIEQVLALFPVLAERQNQLAGTLSGGEQQMLAIARALVASPQLICMDEPSMG
                     LAPIMVDRVMEAIREINQAGTAVLLVEQNAQAALSVADRAYILRDGRIVASGTSDEML
                     ADGRLASAYLL"
BASE COUNT          468 a          829 c          857 g          535 t
ORIGIN      
        1 tacggcgaat aggatgcccc ggaaccctcg ttagagccca cacaggagaa cgccggaccc
       61 agaaatatcc atagatgtgg atagtgcgta gcacacttct tacaggtctt ccaactccgt
      121 gccaaacgag gtcagcagaa atacctgcac aagggcgatt gtgtggctga ccggcgtttc
      181 cgagcggagc cgggtcgcct acctctgacc cggaatgctc gctctgtgag ggatcctgga
      241 tgcaccgcga atcagcggcc taaaggagat aggcgctggc cagcctgccg tcggcgagca
      301 tttcgtcgga ggttccggac gcgacgattc gcccgtctcg gaggatgtag gctcggtctg
      361 ccacggatag tgcagcctgc gcgttctgct ccacaagcag tacagctgtg ccggcctggt
      421 taatctcgcg gatcgcttcc atcacgcggt ccaccatgat tggagctaag cccatggacg
      481 gttcgtccat acatatgagc tgcggtgagg ccactagcgc ccgggcgatt gcgagcatct
      541 gctgttcgcc gcctgagagc gtccctgcga gctggttctg gcgctcggcc agcaccggga
      601 agagggcaag tacctgctcg atctggctct gcgcggctgc gtggttcctg gctctgctcc
      661 cggcttctcg catggctgca gttgccgctt tcgcttgagc ttctgccgcg acctccaggt
      721 tctcgagcac cgtcagctcg tagaacaccc ggcgtccttc gggaacgata ccgatcccca
      781 tccgcacgat ctcattggtg gggcgtccgt ttatgcggcg ccccgcgaag cgtatctcgc
      841 cgctagacgg gcgtagtctg cccattatcg tcttgatggc cgtgctcttg ccggctgcgt
      901 tcgccccgag cagtgcgacg acttcgcctg gacggacttc aatgtcgatc ccttgcagga
      961 cttcagccgg accgtaggac gcgtgcaccc ccgcgagctc caggatgggc gcgactcccg
     1021 atgtagccgc atcggcgctg gcatcggcgt tggcgtcggc atgggcgttg gcgtcggcag
     1081 cggcggcggt cgaagtcggt gcggaggcgg ttttcgccga ccccagatag gcgcggatta
     1141 cttcgggatg ggccgctacc tcctgtggtg tgccggcggc tatgacttcg ccgtggtcga
     1201 gcgcgattac tcggtcgcac gctgcggcta tgaggtccat gtgatgttct atgacgacta
     1261 tcgtgtagcc ccggtcgcga agggcgacga tgtcgtctac catcgctgtg atctcttcgg
     1321 gagtcatgcc ggcagcgggt tcgtccagta gcaggagctt ggcgcctgtt gcgatggcac
     1381 gagctacctc gacccggcga cggtcggggt atgacagatg gcggaccagg gaagcttcgc
     1441 gcgtcaggag cgatgtatcg accagggcca gggcctcccg agcctgggca actgcctccg
     1501 tctcctggcc tgtgtgcgct acggaccgtg ccaacgcccc gagaacgttg ctgcgccccc
     1561 gcaggtgaca gcctgccagg acgttctcga gcaccgactg ggtttcgaat aggcggatgt
     1621 gctggaaggt ccgtgcgacc cccagagatg ctacctgatg cggatcgagt cccgttgcgg
     1681 tttggccctg aacgcacact tctcctgcag atggcttgac gaggcctgtg atcaggttga
     1741 acaacgtggt cttgcctgcg ccgttcggcc cgattatccc caggatctca ccggcatggg
     1801 ccgcgaaact cacgttcgat acagccgaaa gccctccgaa gttgcagctc aggccggacg
     1861 cctcgagtac cggcaccggc acggatccca aagccacagt ggacttgggc tcagcctctg
     1921 ctgcggatga aagtctggcg tcggtcttgg gccccgcccc ggccccggca tcagcttcga
     1981 tataggcgtc agtctcctgt gtcgagcctt gtgccgagct ctgcgccgaa ctctgtaccg
     2041 agtctcgcga gtctgagaga ggcctgcggc caatgcctac cagcttctcg ccctgggcga
     2101 taagcccctg cggacggaac acggctagca gcaccagcgc aaagccgtat atcagcatcc
     2161 ggtatatgct gtaggctcgc agcacctcgg gcaggacttg caggatgagc acgccgagca
     2221 tgatcccggg tatgctgcgg atgcccccga ggatggccat gcagtagagc gcgatggtct
     2281 cggacatggt gaaattctgc gggaagaccg cgccttgcca cgatgcgaac aatgctcctg
     2341 ccaacccggc gatagcggcg gcggttacga atgccgatac ccggtagcgt cgaacgttca
     2401 cacccatgca tgcggcagcc gtctcgtctt cgcgtagcgc gttccatgcg cgtccggtct
     2461 tggagccgat aatgcgcgca attccggcca agatgatcag ggcaaggacc cagatgagga
     2521 tgtacgatga ctccagggag ctgagggtga accctagtat cctgggcgga tcgatggcca
     2581 caattccgtt ggggccgttg gtaatgttga tcggtcggtc catgttgttc agtaggatcc
     2641 gcacgatctg tccgaacccg agggtaacca ttgccatgta gtcgccgtg
//
LOCUS       DAOTAC010000312         2683 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_29501_length_2683_cov_4.585616, whole genome shotgun sequence.
ACCESSION   DAOTAC010000312 DAOTAC010000000
VERSION     DAOTAC010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29501_length_2683_cov_4.585616"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..2478)
                     /locus_tag="PLM74_10840"
     CDS             complement(58..2478)
                     /locus_tag="PLM74_10840"
                     /inference="COORDINATES: protein motif:HMM:NF014513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HON43604.1"
                     /translation="MRSLSRFVQNHPVAVIVATILITVLAALSATRISVSTAVRDFFP
                     STDPEIVALDHIDEIFGGSDYVLIAAKADDVFTPEGLQVLQDITQGIEDIDGVHSVRS
                     VTNMVEVQGTPWGIEVVDLLAEMPTDHGEADAVRRRMEENPQIAGVLLSESGRYVLAL
                     AQLDQGVDAGAVSGRIRAVIDQRRGDITFHLSGSPFLSKAAEEHLKDDLFNLFPFAVA
                     VIVIVLALSFKTISGVLLPLVTVLLSIVWTLGLMGAVGVPLTQMSAVLPVVLLSSGSA
                     YGLHMMHRYYEENGNGKPNGKSNGKANGKADSAATGNGSAGRALESVGIAIILAGVTT
                     VAGFGSNVFSSMTRMREFGGFTAIGVAFALLIALTFLPAVLTVLDRRHGSKHSRIARA
                     SSAAKGGTAARGGRRFLESVGNYVLRKPRVVVIATLALIVWSVGGLSRLKVDTDFATF
                     FDESSTARRDFDLIRTEFGGVDTIQVLVEGDILDPVTLNAMDRAQQEFEKGIGFGKAF
                     SIVDVVKQVSLALHEDDPAWDRIPDSREEVAQYLLLVSMSGDVGIDQMLSLDETHARI
                     QVMVDSTVNSASSGGTLEQARSIVARNLEGVGAIERVYLTGLPFLSESMGKQIMAGQM
                     KSLLLAGIAVAVIVYFTLGRSITSALCMGPIALTILANFGIMGWAGIPVNLVTALVSS
                     IAVGIGIDYSIHIYNRYSEGLKCGLTPDEAIHVTISNTGIAVALNALSVAAGFMVMLA
                     SRFPPVRQFGTLIAVTMAVSAAGALSLLPVTLLAASSRKPAWAHGTKEQNGRGHQGTR
                     QRARGERA"
BASE COUNT          568 a          879 c          697 g          539 t
ORIGIN      
        1 taacgtacgc ccaggacttc cccggagccc cgcaagtcac cgagcccatt ttctccctca
       61 tgcccgttcc cctctcgctc gttgtcgtgt cccttgatgt cctcttccat tttgctcttt
      121 cgtaccgtgc gcccaggccg gcttccggct actcgccgcg agtaaagtca ccggcagcag
      181 cgacagggca ccggccgccg acacagccat agtcaccgca atcaacgtac cgaactggcg
      241 tacaggtgga aatctggatg ctagcatcac catgaacccc gccgccaccg atagcgcgtt
      301 cagtgctacg gctatacccg tattcgatat ggttacatga atggcttcat caggcgtgag
      361 cccgcacttc aggccttcag agtatctgtt gtagatgtga atgctgtagt cgatgcctat
      421 cccgacggct atcgaactca caagagccgt caccagattg accgggatgc ccgcccaacc
      481 catgatgccg aagttggcaa ggatagtcag cgctatcgga cccatgcaca gcgccgatgt
      541 gatcgagcgc cctagagtga aatacacgat taccgcgacc gctattcccg ccagaagcag
      601 gctcttcatc tggccagcca ttatctgctt gcccattgac tccgacagaa acggcagtcc
      661 ggtgaggtac acgcgttcta tggcacctac accttccaga ttgcgcgcga caatgcttcg
      721 agcctgttcg agcgttccgc ctgaactggc tgagttcact gtggaatcca ccatcacctg
      781 tattctggca tgggtttcat cgaggctgag catctgatcg atacctacat ctcccgacat
      841 cgatacgagc agtaggtact gcgcgacctc ctccctagaa tcggggatgc ggtcccacgc
      901 cggatcatcc tcgtgcagag ccaacgacac ctgcttcaca acgtctacaa tggagaaggc
      961 cttcccgaac ccgatgccct tctcaaactc ctgctgcgcc ctgtccatag cattcagggt
     1021 gactggatca aggatgtcgc cctcaaccag aacctgaatc gtatccacgc cgccgaactc
     1081 cgtcctgatg aggtcgaaat cgcgccgcgc ggtgctggac tcatcgaaga aagtggcaaa
     1141 gtcagtgtca accttgagac gggacagccc tcccaccgac catacaatca gcgccaacgt
     1201 agcgatgacc actactctgg gctttcggag cacgtagttg cccacactct ccaggaacct
     1261 tcggccgccc cgtgccgcgg taccgccctt cgcggcagaa ctggcgcgcg caatccgact
     1321 gtgtttcgaa ccgtgcctcc ggtcgagcac agtcagcaca gcaggcagga atgtaagggc
     1381 aatgagcagc gcaaaggcca cgcctatggc tgtgaacccc ccgaattcgc gcatccgcgt
     1441 catagagctg aacacgtttg acccgaaccc ggccaccgtg gttacacccg ccaggattat
     1501 cgctatcccc accgactcga gagctcgacc ggcggaaccg ttcccagttg cggcggagtc
     1561 tgccttcccg ttcgccttcc cattcgattt cccgttcggt ttcccgttcc cgttctcttc
     1621 gtagtaccga tgcatcatgt gcaggccata ggcgcttccc gatgacagca acacaaccgg
     1681 aagcaccgcg ctcatctgcg tcagaggaac cccaactgcg cccatgagcc cgagcgtcca
     1741 cactatgctc agcagcacgg taacgagcgg caggagcaca ccgctgatag tcttgaagct
     1801 caacgcgagc acgatgacga tcaccgccac agcaaatggg aagaggttga acaggtcatc
     1861 tttcaggtgt tcctctgccg ccttactcag gaatggcgaa cccgacaagt ggaaggtgat
     1921 gtcgccccga cgctgatcga tcactgcccg aatccggcca gaaaccgccc ctgcatcgac
     1981 tccttggtcc agttgggcaa gggccagaac gtagcgaccg ctctcggaca gaagcacacc
     2041 tgctatctgc gggttctcct ccatccggcg ccgaacggca tcagcttcgc catgatctgt
     2101 cggcatctcg gcgaggagat ctactacctc aatgccccat ggagtgccct gcacctccac
     2161 catgttggtg accgacctaa ccgaatggac accatcgata tcctcgatgc cctgcgtgat
     2221 gtcctgaaga acctgtaggc cttcgggggt aaacacgtcg tcggccttag ccgcgatcaa
     2281 aacatagtcg gaacctccga agatctcgtc gatgtggtcg agcgccacta tctcggggtc
     2341 ggttgaaggg aagaagtccc gcaccgcggt gctcacactg atcctggtcg cgctgagggc
     2401 ggcaagcacg gttatgagga tcgtcgcaac gattactgcc actgggtggt tctgaacgaa
     2461 cctggaaaga gacctcacgc atttcagctc catttcacgc gccacgccgc cattcatctg
     2521 ccgcactggc gctccaggct gactgacccg tcagtcagcc gccactagta tattgcacca
     2581 accttgcctt gatgtcaacc tttcagcgcg gcgagggtgc gtgtaaggat tgtggcgttc
     2641 agggtcgcag atcccctgca ttcaggcgtg tggtctcgcc agg
//
LOCUS       DAOTAC010000313         2667 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_29720_length_2667_cov_9.833461, whole genome shotgun sequence.
ACCESSION   DAOTAC010000313 DAOTAC010000000
VERSION     DAOTAC010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29720_length_2667_cov_9.833461"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..556)
                     /locus_tag="PLM74_10845"
     CDS             complement(71..556)
                     /locus_tag="PLM74_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HON43605.1"
                     /translation="MLRVEHSLQSERGLTLVELLIVMVLAGLIMGVTWNIMTGAWGAW
                     ARTSTHTSLDMQSEAGMLAVSSKIRIATSVDAADPASPVIKVKGVSGETVYSFEVEGT
                     DLKMITTAPGGSSSTATVARDLASFQVIETEYAGSYTVSITCTRSGMSKTASTQVTIR
                     R"
     gene            complement(525..929)
                     /locus_tag="PLM74_10850"
     CDS             complement(525..929)
                     /locus_tag="PLM74_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HON43606.1"
                     /translation="MKGMADESGYSLVEVLVAIVFIGVAIAALFTAIMQGVYVQKAAE
                     MEAVALNLANAAIERYRSYFNAGNYDYLVTRSTEEEEAELAELGRSQFRRAIVVALQA
                     AAPDRTWTIAANVAYDLKGKSRDVTCRTLFAE"
     gene            complement(934..2391)
                     /locus_tag="PLM74_10855"
     CDS             complement(934..2391)
                     /locus_tag="PLM74_10855"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011929062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HON43607.1"
                     /translation="MSSVWKGPLEMIDEFRWRIPSHYKQGMRADAVVVTDSEGLEVAR
                     DGEALEQLANSACLPGINGPALAMPDIHSGYGFPIGGVGAMRLEDGVITPGGIGFDIN
                     CGVRLIRTDLELSDAAPKVQSWIDRLYRDVPSGLGTRGPIQLSASEVRKVLERGAQWA
                     VHSGYGSEADLECTEDGGKMEAADSAAVSARAMERGMPQLGTMGSGNHFVEIQVVEEV
                     IAPDVAKVLEIHENQIVMMIHTGSRGLGHQVCTDYLATMNAALKKYGITVPDRQLACA
                     PFESPEGQRYFAAMACAANFAWANRQCITHWVRQATAREFGSTPESLGMTLLYDVCHN
                     IAKIEEHSVDGRPRKLCVHRKGATRAFPPGHKDVPAAYRGVGQPVIVPGDMGRNSYVL
                     VGTQKAMEVSFGSTCHGAGRLMSRNEAMKAQSPAQVRQSLAQAGVYARAASKSALAEE
                     APEAYKDVNDIVDITHKAGISMKVARLRPLGVVKG"
     gene            complement(2407..2607)
                     /locus_tag="PLM74_10860"
     CDS             complement(2407..2607)
                     /locus_tag="PLM74_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43608.1"
                     /translation="MEKDEGEGSARLEAEADGIVLTEDAIHSAADQGAILGLVPKAAS
                     YHMIEVAELADGRWTARLVLDA"
BASE COUNT          500 a          886 c          702 g          579 t
ORIGIN      
        1 aaccagcact gacagcacga ctgaacctga ttcagacgaa tgatcgcgcg ccaatcggct
       61 caccccgctt tcacctcctg atggtaacct gtgtggatgc ggtctttgac ataccggaac
      121 gtgtgcatgt gatggacact gtgtaagagc cggcgtactc agtctcgatc acctggaaag
      181 atgccaagtc tcttgcaacc gtagcggtgc ttgacgaacc gcccggcgca gtcgtgatca
      241 tcttaaggtc ggtcccttca acctcaaagg agtagacagt ctcgcccgaa acgcctttca
      301 ccttgatcac aggacttgcc ggatcggcgg cgtcgacaga cgtggctatt cttatcttgg
      361 acgagacggc cagcatgccc gcttcagact gcatgtcgag agaggtgtgg gtggacgtac
      421 gcgcccacgc gccccacgct ccagtcatga tgttccaggt cactcccatg atgagccctg
      481 ccaacaccat gacgatgagc agctcgacta gtgttagccc tctctcactc tgcaaagagt
      541 gttcgacacg taacatcacg agacttcccc tttaggtcat atgcgacatt ggccgcgatg
      601 gtccaggtgc ggtcgggcgc cgcggcctgc aaagcgacga caatcgccct gcgaaattgc
      661 gatctgccga gctccgcaag ctcggcctct tcttcttcgg tgcttcttgt gaccaagtag
      721 tcgtagtttc cggcattgaa atatgatcgg taccgctcta tcgccgcgtt ggccaggttc
      781 agggcgaccg cttccatctc ggctgccttc tgcacgtaca ctccctgcat aatcgcagtg
      841 aagagggcgg caatcgcaac gcctatgaac actatggcta cgagcacctc gactagagag
      901 tatcccgatt catcggccat gcctttcatt cttctacccc ttcactactc ccagcggcct
      961 aagccgcgcc accttcatgg atatcccggc cttgtgagta atgtcgacaa tgtcgtttac
     1021 atccttatag gcctcgggcg cctcttccgc gagcgcgctc ttgctggccg cgcgggcata
     1081 cacccccgcc tgtgcaaggg attgcctgac ctgggcggga ctttgcgcct tcatggcttc
     1141 gttgcgactc atcagacggc ctgctccatg acaggtggag ccaaacgaaa cttccatcgc
     1201 cttctgcgta cccacgagaa cataggagtt gcggcccatg tcgcccggaa cgatcaccgg
     1261 ctggccgacc cctcgatacg ctgcaggcac atccttatgc ccaggcggga aggcgcgcgt
     1321 ggcccctttt ctgtggacgc agagcttccg aggacggcca tctacagagt gttcttcgat
     1381 cttggcgatg ttatggcaca catcgtacag cagcgtcatg cccaggcttt ccggagtcga
     1441 cccgaactcg cgcgcggtag cctgcctgac ccaatgagtg atgcactggc gattcgccca
     1501 ggcaaagttg gccgcgcatg ccattgccgc gaaatacctc tgcccctcgg gcgactcgaa
     1561 tggggcgcac gctagctgcc tatcgggaac ggttattccg tatttcttca gggctgcgtt
     1621 catggtagcc agatagtcgg tgcacacttg gtgccccagc ccgcgtgacc ctgtatgtat
     1681 catcatcacg atctggttct catgtatttc gagtactttc gccacgtccg gcgcaattac
     1741 ctcttcgacc acctgtatct ccacgaagtg gttgccggac cccatagtgc ccaactgcgg
     1801 catcccgcgc tccatggccc gtgcgctcac ggcagctgag tccgccgcct ccatctttcc
     1861 cccgtcctcg gtgcactcca ggtcggcctc gctgccgtag ccggaatgca cagcccactg
     1921 ggctccgcgt tcgaggactt tgcggacctc gctggcgctg agctgaatgg gacctcgcgt
     1981 gccgagcccg gatggcacat ctcggtacag ccggtctatc caggattgga ccttgggcgc
     2041 cgcatcggaa agctccagat ccgtcctgat cagccttacc ccgcagttta tgtcaaagcc
     2101 gatccctccg ggcgtgatca cgccgtcttc caggcgcatc gcccccacac caccaatggg
     2161 aaagccgtac cccgaatgga tgtccggcat ggccagagcg ggcccgttta tgcctggaag
     2221 gcaggccgag ttggcgagct gctccagagc ctctccatcg cgggcaacct ccagcccttc
     2281 gctgtcagtg accacaacgg catccgcgcg cattccctgc ttgtaatggc tgggtatgcg
     2341 ccatcggaac tcatcgatca tctcaagcgg gcctttccac acactcgaca catgctccac
     2401 ctcctctcaa gcatccagga ctagccgggc cgtccaccta ccatcggcca gttccgccac
     2461 ttctatcatg tgataagaag ccgccttggg cacaagcccc aatatcgcac cttggtcggc
     2521 tgcggagtgg atcgcgtctt ccgtcagcac aatgccatca gcctccgcct caagacgcgc
     2581 actgccctcg ccctcgtcct tttccatgta tgtgacccat cttcagcacc cttgcgcatt
     2641 caggttctga gttttagggg tctcgcg
//
LOCUS       DAOTAC010000314         2659 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_29840_length_2659_cov_4.257296, whole genome shotgun sequence.
ACCESSION   DAOTAC010000314 DAOTAC010000000
VERSION     DAOTAC010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29840_length_2659_cov_4.257296"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            390..1349
                     /locus_tag="PLM74_10865"
     CDS             390..1349
                     /locus_tag="PLM74_10865"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HON43609.1"
                     /translation="MPEVVYSEETIRTQDGTELFCRVWLPVGPSGANSTGADAGDGTA
                     AVATRGATATATAAPAADVAGRARAVVVICHGQGDHSGRHTHVAQFFAERGYAVYAYD
                     HRGQGKSGGVRGHVDRFARYYDDLWLVIRTAKERHSGLRVFVIGQSLGGLIALGYAER
                     RPKTITGVVASSPGFIYLEKVPRWKKALAPVVSWIAPCMAFETGIQAEHISRDPAVVA
                     AYKADKIRYPKVTARYYVEWDRATRAVFEGSKSLAVPCLFMPAGADQIISAQATVDFY
                     NNDSHPDKKLVVWEGLYHDIFNEPEKEKVLETAAAWIAERDGR"
     gene            1351..2247
                     /locus_tag="PLM74_10870"
     CDS             1351..2247
                     /locus_tag="PLM74_10870"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HON43610.1"
                     /translation="MRQALELTAEDIQRYSRHLCLAGVGEEGQRRIRSARALVVGVGG
                     LGSPIALYLAAAGVGVLGLVDPDVVELSNLQRQIAHGTGSLGVPKVESATSRIGDLNP
                     GVRVEAMAVKLGPHNAAQLVAGYDVIVDALDSLRDRFLLSDACVAMGKPMVHGAVSGW
                     MGEAMTYVPGQGPCYRCVFGGGAAARYATACDADSAVSVGAYSGAEDTPEDLGALSPE
                     RVGVLSSAPGVIGAIEATEVLKLITGAGEPLVGRLLLWDGMRMRFDQIVVMADPVCSA
                     CETGGPGKRGQCHDYADNGCRD"
     gene            2216..>2659
                     /locus_tag="PLM74_10875"
     CDS             2216..>2659
                     /locus_tag="PLM74_10875"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HON43611.1"
                     /translation="MITPTMDVATEGRRDVNTARAVKSLSEAVQVRTISFSDRALMDH
                     GQFERFHALLERLYPLVHRGLEKTALGHNLLYLWKGAEAAEGNQGGQGKRPVALMSHM
                     DVVPADEDGWEYPPFSGAVADGHVWGRGSIDMKFGLITILEAIEEM"
BASE COUNT          514 a          670 c          945 g          530 t
ORIGIN      
        1 tacgttgtgt catgcatcaa cgattgtcta gattctcgac atgtccggga aaatgcgtcg
       61 attgtatcga catatcccaa aaagccaggt agacaggcat ttcaccgcaa accagcagcg
      121 tgcggcaagt atatgctctt gagtcgacga ctacccagga ccgatcatca gccacaaaag
      181 atcgaccacc ggcaaaggta atctcctgcg caagaagttc acggcccggc tgcttgtcca
      241 tagattaggc atgaagttgt ggaggcgcgg agccgggcgt tcgccgcgaa caggcccttc
      301 gaggcggtag gtaaggaact gagcatcatc tgaggttgct gggcgatggg gcctgcgggt
      361 ctggccgggc gcgaaaggag ggaccgatat tgccagaagt cgtctattcc gaagagacta
      421 tcagaaccca ggatggaacc gagctgttct gccgggtgtg gttgccggtc gggcccagcg
      481 gggcaaattc taccggagca gacgcgggag acggcacggc cgcggttgcg actcggggcg
      541 cgactgcgac tgcgactgca gctccggctg ccgacgtggc gggtcgagcg agggctgtag
      601 tggtgatctg ccacggtcag ggtgatcatt cgggaagaca cacgcatgtg gcgcagttct
      661 tcgcagagcg gggctatgcc gtttacgcct atgaccaccg tgggcagggc aagtccggcg
      721 gtgtgcgcgg ccatgtggat cggtttgcgc ggtattacga tgatctctgg cttgtgatcc
      781 gcacggccaa agagcgtcat tccgggctca gagtgttcgt catcgggcag tccttgggag
      841 gcctcatcgc tctagggtac gccgagcggc ggcccaagac aatcaccggt gtcgttgcca
      901 gttcgccagg cttcatctat ctagagaaag tgccgcgctg gaagaaggcg ttggcgccgg
      961 tagtctcctg gatcgcgccc tgcatggcgt tcgaaaccgg tattcaggct gagcacatat
     1021 cgcgcgatcc cgcggttgtg gcagcataca aggccgacaa gatcagatac ccgaaggtga
     1081 ctgccaggta ttacgttgag tgggatcggg caacgcgcgc cgtcttcgag ggctcgaagt
     1141 ctctcgcggt accatgcttg ttcatgccgg ccggcgccga ccagattatc tctgcgcagg
     1201 ctaccgtgga cttctacaac aacgattctc accctgacaa gaagctggtc gtgtgggaag
     1261 gcctgtatca cgatatcttc aacgagccgg agaaggagaa agtgttggag actgccgcgg
     1321 cctggattgc cgagcgggat ggacggtagg atgaggcagg cactcgagct gacagctgag
     1381 gatatccagc gatatagcag gcatctgtgc ctcgcaggtg ttggcgagga aggtcagagg
     1441 agaattcggt cggcccgggc cctggttgtg ggtgtgggag gactgggttc tcctattgcg
     1501 ctttatttgg ccgcggccgg cgtcggcgta ttgggattgg tggacccgga cgtggtggag
     1561 ctgtcgaact tgcagcgcca gatagcccat ggcaccggca gcctgggcgt gccaaaggtt
     1621 gagtcggcta ccagtcggat cggggacctg aacccaggcg tgcgtgttga agccatggcg
     1681 gtgaagctgg ggccgcacaa tgccgctcag ctagtggcgg gctatgacgt gattgttgat
     1741 gccctagata gcctgcgcga tagatttctc ctaagcgatg cgtgtgtggc tatgggaaag
     1801 ccgatggtgc atggcgctgt gtcggggtgg atgggtgagg ccatgaccta cgtgcccggg
     1861 caggggccct gctacaggtg cgtgtttgga ggaggcgcgg cggcgaggta cgcaacggcg
     1921 tgcgatgccg acagcgcggt ttcggtcgga gcctactcgg gggcggagga caccccggag
     1981 gatttgggtg cgctttctcc ggagcgtgtg ggtgtgctgt cgtcggcgcc gggggtgata
     2041 ggggccattg aggccacgga ggttctcaag ctgatcacag gcgcaggcga accgctcgta
     2101 ggcaggctgc tgctgtggga tggcatgagg atgcggtttg atcagatagt ggtgatggcc
     2161 gatcctgtgt gtagtgcatg tgagacaggc gggccgggaa agagggggca atgccatgat
     2221 tacgccgaca atggatgtcg cgactgaagg caggagggat gtcaatactg ccagggctgt
     2281 gaagtcgctt tcggaggcgg tgcaggtcag aaccatatcg ttttcggacc gggcgcttat
     2341 ggaccacggc cagttcgagc gttttcatgc actcctggag aggttgtacc cgctcgtcca
     2401 taggggactg gagaagactg cgctcggcca caacttgctc tacctgtgga agggcgcaga
     2461 ggctgccgag ggtaaccagg gcggacaggg caagaggcct gtggcgctga tgtcgcacat
     2521 ggatgtggtt cccgcagacg aagacggatg ggagtatccg cccttctcgg gcgcagtcgc
     2581 cgatggacac gtgtggggtc ggggctctat cgatatgaag tttgggctca ttactatcct
     2641 cgaggccatc gaggagatg
//
LOCUS       DAOTAC010000315         2656 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_29886_length_2656_cov_3.924644, whole genome shotgun sequence.
ACCESSION   DAOTAC010000315 DAOTAC010000000
VERSION     DAOTAC010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29886_length_2656_cov_3.924644"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="PLM74_10880"
     CDS             <1..354
                     /locus_tag="PLM74_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43612.1"
                     /translation="PALFEYIDFRRPPDRIQIAKIGGYPVGPGMAPGRECDRISHNMA
                     VSGNTAQAMEYPSTNPPHPRPPLTPKAPFAAPIRRLEAPAQMHVLKNCAYRQMYRQKL
                     TACLLGYPRGYALEL"
     gene            789..1805
                     /locus_tag="PLM74_10885"
     CDS             789..1805
                     /locus_tag="PLM74_10885"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020492402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HON43613.1"
                     /translation="MYTKISGTGSALPENRVTNRDFERLVDTSDEWITTRTGISERRI
                     SSGENTSDFAAQAAANALSSAGVGPLDVDLIIAATVSPDSFTPTVSCQVQKALGISRA
                     TCFDLWAGCTGFIFALMTADSLIRSGLSGTALVIGAEVLSKLVDWSDRATCVLFGDGA
                     GAVVLSQSASPGILAHYTGSDGTRGNVLRIDGVPLVNPWTPSAHAGTHPSPSHPSSVI
                     RMDGQEVFRFAVTAMQQSIRRVCELSGIELDDVPYVIPHQANDRIIESVAGRMKIPRN
                     RFYTNLDRRGNTSAASIPIALDEMNRAGMLKPEMPLLLVGFGGGLTWGASMVRWMPNF
                     KGDS"
     gene            1833..2078
                     /gene="acpP"
                     /locus_tag="PLM74_10890"
     CDS             1833..2078
                     /gene="acpP"
                     /locus_tag="PLM74_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HON43614.1"
                     /translation="MVFDQLKAIIVDQLDVAPEDVSPESSFADDLGADSLDMVELIMA
                     IEEKMEVHIEQKEARNLTTVGDVLAFMEAQKAAGVTK"
     gene            2075..>2656
                     /locus_tag="PLM74_10895"
     CDS             2075..>2656
                     /locus_tag="PLM74_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HON43615.1"
                     /translation="MTVGRLGQRLTRLVGVTYPVIQGGMAWVADAQLAAAVSNAGGLG
                     VIASAHLTPDQLRSEIRAARKMTAKPFGVNIMLMSPNAGAAARICCEEHVAVVTTGAG
                     HPGEFMEDFTSAGIIVMPVIASTAQAKRAASAGAHAVIAEGYEAGGHVGELTTMALVP
                     QVVDAVDVPVVAAGGIADGRGAAAAFCLGASGVQ"
BASE COUNT          541 a          824 c          804 g          487 t
ORIGIN      
        1 ccggcacttt ttgagtacat cgatttcaga agaccgccgg accgcatcca gatcgcgaaa
       61 atcggggggt atccggtcgg tcctggcatg gcaccgggca gggaatgcga caggatatcc
      121 cataacatgg ctgtatccgg caataccgcg caagccatgg aatacccctc caccaatcca
      181 ccacatccta ggccgccgct gaccccaaaa gcacctttcg cagcaccgat ccgccggctg
      241 gaggccccgg cccaaatgca tgtactcaaa aattgcgcct atcggcagat gtatcggcaa
      301 aaactgacgg catgcctgct tggatacccc cgggggtatg cccttgagct gtagtaatcg
      361 ccgttctgcc ctcggatcta tccctggatc tatccctcgg atctatcccc ggatctatcg
      421 caggtcccct tctgccccag cccctctcac tgtgatcctg gcgaggtcgc ttgccgaagg
      481 ccagacccgg agtgggagtc ggatgggttg agtagggcgc caaacgccgg atcggcgcgg
      541 cgccggatcg gcgcgacccc ggaacctggg aagccccgga acctgggagg cccgcggctc
      601 tgagagcaac aatcccttca tgcacctacc ccattgacat aagaccctcc ccgcaatata
      661 ctttgacaga cgaatagttt ggttctcaaa acatcttcct cagtgacccc aatgatctcc
      721 ccgccgggca gtctcccatc tggtctcgac tatgggaggc gccatgcgag gcatcggagg
      781 catggagcgt gtacacgaag atctcgggaa ccggcagtgc gctgcctgag aaccgcgtga
      841 ccaaccgcga ctttgagcgc ctcgtcgaca catccgacga atggataacc actcgcacag
      901 gcatatccga gcggcgcata tcttccggag agaatacgtc cgacttcgcc gcgcaggcgg
      961 cggcaaacgc gctgtcgagt gcaggtgtcg gcccgctcga cgtggatctg atcatagccg
     1021 ccaccgtatc gcccgacagt ttcaccccta ccgtatcgtg tcaggtccag aaagcgctgg
     1081 ggatttccag agccacctgc ttcgatctgt gggcaggttg cacggggttc atcttcgccc
     1141 tgatgacagc cgattcgctg atccgttcgg gcctgtccgg cactgccctg gtcatcggcg
     1201 ctgaagtcct ttccaagcta gtagactgga gcgaccgtgc tacctgcgtg ctgtttggag
     1261 acggcgcggg agctgtcgtc ctcagccagt ctgcaagccc agggattctg gctcactaca
     1321 ccgggtcgga cggcactcgc gggaatgttc tccgcataga cggggttcca ctggtcaatc
     1381 catggacccc cagcgcccac gccggcaccc acccatcgcc atcccacccg agcagcgtca
     1441 ttcgcatgga cgggcaggag gtcttcagat tcgctgtgac cgctatgcaa cagtccatac
     1501 gacgcgtctg cgagctttcc ggcatagagc tggacgatgt cccctatgtc atcccgcatc
     1561 aggccaacga cagaatcatc gaatccgtgg ccggtaggat gaagatcccc cgaaaccgtt
     1621 tctatactaa cctggaccgg cgtgggaata cttccgctgc cagcatacct attgcgctgg
     1681 acgagatgaa ccgcgccgga atgctcaaac ccgagatgcc gctgctgctt gtgggctttg
     1741 gcggcgggct gacgtggggc gcaagcatgg tcaggtggat gcccaatttc aaaggagact
     1801 catgaaaccg aaagactgag gagggtctca agatggtatt tgaccagttg aaggcgataa
     1861 tcgtagacca actagacgtt gccccggagg atgtgtcccc agagagcagt ttcgccgatg
     1921 acctcggcgc cgactccctc gatatggtcg agctcatcat ggcgatcgag gagaaaatgg
     1981 aagtccacat cgagcaaaag gaagcaagga acctgacaac cgtcggggat gtgctggcat
     2041 tcatggaggc acagaaagcc gcaggggtca ccaaatgacc gtggggcgtc tcggccaaag
     2101 gctgacgcgc cttgttgggg tgacatatcc cgtgatacag ggcggaatgg cctgggtagc
     2161 ggatgcgcag ctggcggcgg ctgtatcaaa cgcgggtggg ctgggagtca tcgcctcagc
     2221 ccacctcacc cccgatcagc ttaggtccga gatccgcgca gcccggaaga tgactgccaa
     2281 gccattcggc gtcaacataa tgctgatgag tccgaatgct ggcgcggcgg cgcggatctg
     2341 ttgcgaggag cacgtggccg tcgtcaccac cggcgccggc catcccggcg agttcatgga
     2401 ggactttacg tccgccggca tcatcgtcat gccggtaatc gcgtccacgg cccaggccaa
     2461 gcgggcagcg tccgccggcg cacacgctgt gattgccgaa ggatacgagg caggcgggca
     2521 cgtcggagag ttgactacca tggcgctggt gccccaggtc gtggacgcag tggacgtgcc
     2581 ggtcgtggcg gccggtggca ttgcggacgg ccgaggagcg gcagcagcct tctgcctcgg
     2641 cgcttccggg gtgcag
//
LOCUS       DAOTAC010000316         2654 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_29918_length_2654_cov_5.599461, whole genome shotgun sequence.
ACCESSION   DAOTAC010000316 DAOTAC010000000
VERSION     DAOTAC010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29918_length_2654_cov_5.599461"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..888)
                     /gene="codY"
                     /locus_tag="PLM74_10900"
     CDS             complement(103..888)
                     /gene="codY"
                     /locus_tag="PLM74_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HON43616.1"
                     /translation="MSSLLERTRRVNRLIQNAAGYSVDFRELAEVLADTMSCQAFVIA
                     RNGKLLGHSLTIVSEAGDEQPETLETGFVPRESNEELLRVPDTSPNQMPSGRLTIFQD
                     ELLGGSESCRITVVPVHGGGERVATLVLLTADGSLDEDDIVIAEYGAAVIGMEILRSK
                     SEKMEEEARKRAAVQMALDTLSYSEQEAVEHIFDELAGDEGLLVASKIADRVGITRSV
                     IVNALRKFESAGVIESRSLGMKGTYIRVLNDRLFDELDKLRAR"
     gene            complement(1009..2406)
                     /gene="hslU"
                     /locus_tag="PLM74_10905"
     CDS             complement(1009..2406)
                     /gene="hslU"
                     /locus_tag="PLM74_10905"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HON43617.1"
                     /translation="MENLTPRKIVEELDKYIVGQAQAKRAVAIALRNRYRRLALSGEM
                     QEDIIPKNILMIGPTGVGKTEIARRLARLVKAPFIKVEATKFTEVGYVGRDVDSIIRD
                     LVEVSIRMVRSEKTGAVDERARVHGEERLLDVLCPLPVREKPVVNPFAALFGAQHSPE
                     PSDSAAFESKLAAARARRREVREQLSRGELEDQVVEIEVDDSSPGMIEVFSGSGIEEL
                     GVNLQDVLGGMLPRKKRRRKVPVHEARRILTAEEATKLIDMDEVTAEGIARAEQSGIV
                     FLDEIDKIAAKSHGAGPDVSREGVQRDILPIVEGCTVQTKHGPVKTGHILFIAAGAFH
                     MSRPSDLIPELQGRFPIRVELTSLAQADFVRILTEPKNALTKQYAALLSTEGIDLSFT
                     PDGIDEIARIADDVNSSAENIGARRLHTILEKVLEDVAFNAPEESGPVIVDAAYVRDR
                     LAGVIARSDFSDYVL"
     gene            complement(2423..>2654)
                     /locus_tag="PLM74_10910"
     CDS             complement(2423..>2654)
                     /locus_tag="PLM74_10910"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014043074.1"
                     /note="heat shock protein involved in degradation of
                     misfolded proteins; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HON43618.1"
                     /translation="IVTDGQRMLVLSGSGEVLEPDDNIAAVGSGGAYALAAARALMRN
                     TDLAAAEIVRQSLEIAADICIYTNNRIIIEEI"
BASE COUNT          512 a          843 c          713 g          586 t
ORIGIN      
        1 cgcggcccga atcggaagcc gtgtgtagtc gcacaacggt tagtatagca cagcccgaag
       61 cgcctcgcaa tccgcacccg gactgtaccc ggactgcgta cgtcaccgtg ctcgaagctt
      121 gtccaattcg tcgaagagtc tgtcgttgag aactcgaatg tacgtgccct tcatcccaag
      181 cgacctggac tcaatcaccc cggcgctctc gaacttgcga agagcgttca ctattacgga
      241 tctcgtgatt ccaaccctgt cggcgatttt ggaggcaacg aggaggccct catccccggc
      301 aagctcatcg aatatgtgct ccactgcttc ctgttccgag taggatagcg tatccaacgc
      361 catctggaca gccgcgcgct tgcgcgcctc ctcctccatc ttctcgctct ttgacctcag
      421 gatctccatt cctatgaccg cagctccgta ctcggcgatc acgatatcgt cttcgtccag
      481 agagccgtct gccgtgagca gaacgagcgt agcgacgcgc tcgcccccgc catggaccgg
      541 cactaccgta atgcggcagg attcggaacc tccgagcagt tcgtcctgga agattgtgag
      601 cctccccgat ggcatctggt tcggcgatgt gtcaggcacc cggagaagct cctcgttgct
      661 ctcccgaggc acgaacccgg tttccaaagt ctccggctgc tcatcgccgg cctcggagac
      721 gatggtcagc gaatggccga gcagcttgcc gtttcgagcg atgacaaacg cctggcacga
      781 catggtgtcg gccaacacct cggcaagctc cctaaaatcg actgagtagc cggcagcgtt
      841 ttgaatgagg cggttgactc tgcgcgtgcg ctccagcagt gaactcacat tggaacctcc
      901 tctcacatac cacgctcaag atggcgtgtg ctgcaaattg actcagcagt gatgctcttt
      961 tatattgtcc aaagattcca gtcccaaaac aggccatagc gcgctcaact acaaaacata
     1021 gtcagagaag tcactacgag caatgacccc cgcaagacgg tcccttacgt aggctgcgtc
     1081 tacgatgact ggacccgact cttccggagc attgaacgcc acatcttcaa ggactttctc
     1141 gagtatggtg tggagcctgc gcgcgcctat gttctcagcg ctggagttga cgtcgtcggc
     1201 gatgcgtgcg atctcgtcta tgccatcagg ggtgaacgac aggtcgatgc cttcggtaga
     1261 aagcagagcc gcatactgtt tggtcagcgc gttctttggc tccgtcagaa tgcggacgaa
     1321 gtctgcctgc gctaggctcg tgagttccac ccgtatggga aacctgcctt gtagctcggg
     1381 gatgaggtcc gaaggtctgg acatgtgaaa agccccggct gctatgaaca ggatgtgccc
     1441 cgtcttcacc gggccgtgct tggtctgaac agtgcatcct tccacgatcg gaaggatgtc
     1501 acgctgcaca ccctcgcgcg acacatccgg acccgcgcca tgactcttgg ccgcgatctt
     1561 gtcgatctcg tctaggaaga cgatgcccga ctgctcggcc cgggctatgc cttctgccgt
     1621 gacttcatcc atgtcgatga gtttggtggc ctcttcagca gtgaggatcc tgcgcgcttc
     1681 gtgcacgggc actttgcgac ggcgcttctt gcgcggaagc atcccgccga gcacatcctg
     1741 gagattcacg cccagctcct cgatgccgct tcctgaaaag acctctatca tcccgggcga
     1801 cgagtcgtct acttcgatct cgaccacttg atcctcgagt tcgccgcggg agagttgctc
     1861 tctgacctcg cgccttcgcg cccttgccgc agccagcttc gactcgaagg cagcgctatc
     1921 agatggctcg ggagagtgct gggctccaaa cagagcggca aacgggttta ccacaggctt
     1981 ctctctgacc ggaagtgggc aaagcacatc aaggaggcgt tcctcgccgt gaacccgtgc
     2041 acgctcgtca acggcgccgg tcttctcgga tctgaccatt cgtatggaca cctcaacaag
     2101 atcacgtatg atgctgtcga catcgcggcc gacatagccc acttccgtga atttggttgc
     2161 ctcgaccttt atgaaggggg ccttgaccag tctcgccagt ctgcgggcta tttccgtctt
     2221 ccccactcct gtagggccta tcatcaagat gttcttgggg atgatatcct cctgcatctc
     2281 accggataga gccagcctgc gatagcggtt acgcagagct atggctacag ccctcttggc
     2341 ctgagcctgc cccacgatgt acttgtcgag ttcctccacg atcttgcgcg gagtcagatt
     2401 ctccatatca tcgcctcccg gactagatct cttcaatgat tatgcggtta ttggtgtaga
     2461 tgcatatgtc ggccgctatc tccagcgact gccgcacgat ttcggccgcc gcgaggtccg
     2521 tgtttcgcat cagggctcgg gccgccgcta aggcgtacgc gccccccgaa cccaccgccg
     2581 caatattgtc gtccggctca agtacttcgc cgctgcccga caggaccagc atccgctgac
     2641 cgtccgtcac tatc
//
LOCUS       DAOTAC010000317         2641 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_30123_length_2641_cov_4.831787, whole genome shotgun sequence.
ACCESSION   DAOTAC010000317 DAOTAC010000000
VERSION     DAOTAC010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30123_length_2641_cov_4.831787"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1204)
                     /locus_tag="PLM74_10915"
     CDS             complement(<1..1204)
                     /locus_tag="PLM74_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43619.1"
                     /translation="MCAAIASLTGLYALATDALGLQCIWTEPASRVLSSWASFVSSQP
                     FSGGPTYLGLHFVFAYILGCLAVFAVGARLGRRAALNLGAALGGGIAGYLVLLTTFAR
                     LTRLTGLWFLALTSCLALMFAAMIAVYASVVSREPVAAARPKLSEALIGVAVASLIAS
                     IATGIIESGQARRSVMFLSEQSANFRPTSPLDFSDKFTPSFGWLAVYLEGTGYEVSWG
                     DLSGDDLERVQTLVVINVVDKLSGEAQEAVLRFLNDGGSLLVLADHTLYPDGSHPLDF
                     VMNPAGMALNFDSARSLFDSGWGQPNLEFRQHPVTLGITDEAQTQIGTGGSLRVRPPA
                     RTLVAAKIRFVDDPNMNNPGQGYFGDLKFSPGERLGDIPLVAAGRFGSGMDTSVGFSS
                     APEKALRQP"
     gene            complement(1210..1539)
                     /locus_tag="PLM74_10920"
     CDS             complement(1210..1539)
                     /locus_tag="PLM74_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43620.1"
                     /translation="MSPIGRTGDRVSRTNLRPALVFILAAVLLDGPTMFALPVKAVWS
                     ALFATLIWLSFPWAHKGPATCGRSPVQQRRSQQSGWAIHGDGSRLQRLHGFRSSPLMT
                     DSASIPS"
     gene            complement(1683..>2641)
                     /locus_tag="PLM74_10925"
     CDS             complement(1683..>2641)
                     /locus_tag="PLM74_10925"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HON43621.1"
                     /translation="ADNVLRDLGENHFPVAISPEFDYVVLMHRDDRDYGSYRLYNVKT
                     GTATLLLSSDIPIEDVGHTYPVSFSASDGATVFAYLTVPLGKSPRNLPLLINVHGGPQ
                     ARFTWAPNEHALLASNLGIAVLSVDFRFSSGYGNEYTDTASQNMLLAQQDVCEGVQWA
                     VENGIADPKCIGIMGHSYGGFISFYQAAVHPDTYKAVISLMGVWDWTDLGVELTDGAP
                     VPDYHKCSAPEPHTALARVLSPSSYVDALKAPILIVYSGSDDAVYPSQNIRAIRELTE
                     AGNTPRAVYLPNDGHMPGTAESITETFEAMRKFLVERLLEEN"
BASE COUNT          561 a          774 c          813 g          493 t
ORIGIN      
        1 agggctgcct gagggctttt tccggcgcgc tcgagaaccc cacactcgta tccatgcctg
       61 agccgaatcg gccggccgca accaggggta tgtccccgag cctttcaccg gggctgaact
      121 tcaggtcgcc gaagtagccc tggccgggat tgttcatgtt cggatcgtct acgaagcgta
      181 tctttgctgc gacaagagtc cttgccggcg gcctgacccg cagggaacct cctgtgccga
      241 tctgcgtctg ggcttcatca gtgatcccaa gggttacagg gtgctgacgg aactcgagat
      301 tgggctgccc ccaccctgag tcgaaaaggc tcctcgctga gtcgaaattg agcgccatgc
      361 ccgcaggatt catgacgaag tcgagcgggt gtgaaccgtc cgggtaaagg gtgtggtcgg
      421 caaggaccag gagagatccc ccgtcattaa ggaatctcaa aaccgcctcc tgcgcttcgc
      481 ctgagagctt gtctactacg ttaatcacaa caagagtctg cacgcgctca agatcgtcgc
      541 cagacagatc gccccaagag acctcgtagc cggtcccctc caaatagaca gcgagccatc
      601 caaaggaggg agtgaactta tccgaaaagt ccagaggaga tgtcggccgg aagttggcgc
      661 tctgctccga cagaaacatg actgacctgc gcgcctggcc tgactcgatg atccctgtgg
      721 caatggaggc gatgagagac gccacggcga cgccgatcag tgcttccgag agcttcggtc
      781 gggcggcagc gacgggttcg cggctgacga ccgaggcata gacggcgatc atggccgcaa
      841 acatcagtgc caggcaggag gtgagcgcga ggaaccataa ccccgtaagc cgcgttaagc
      901 gggcgaaggt tgtcaggagc accagatatc ccgcgatccc gcccccgaga gcggctccca
      961 ggttcagggc cgcgcgccgg ccgagccgcg cgcccacggc gaatacggct aggcagccga
     1021 ggatgtatgc aaagacgaaa tgcagcccga gataggtggg gccgccggag aacggctgtg
     1081 atgaaacgaa cgatgcccat gaggacagga cgcgcgatgc cggctcagtc cagatacact
     1141 ggagtccgag agcgtctgta gccagtgcat atagccctgt caatgacgcg atggcggcgc
     1201 acacggtgtc tagctgggga tcgatgccga atccgtcatg aggggcgaag aacggaatcc
     1261 atgtagccgc tgcaagcgcg agccatctcc atggatagcc cagccagact gctgcgagcg
     1321 ccgctgctgc acaggcgagc gtccacaggt tgcggggccc ttatgcgccc atggaaacga
     1381 aagccagatc aaggtcgcga agagcgccga ccatacggct ttcacaggaa gggcgaacat
     1441 ggtaggccca tcgagcagga ctgcagccag aatgaagacc agagccggcc tgaggttggt
     1501 acggctcaca cggtctccag tccgcccaat cgggctcaca gatgcgcccc ccagcgtgag
     1561 aaccggatat tgttgcagaa tgcttgagta tacatcaagc ttaacccggc gcccgcatga
     1621 agtaaggagt gttatagcca gtgggcgcac gatgacgcgg ggacggcctt ggcgcgagtg
     1681 gttcagttct cttcgagtaa cctttcaacc aggaatttgc gcatcgcctc gaaggtttcg
     1741 gttatgctct ccgccgtgcc cggcatgtga ccgtcattgg gcaagtacac tgcccgggga
     1801 gtgtttccgg cctctgtaag ctctctgata gcccgtatgt tctggcttgg atacactgca
     1861 tcgtcactgc cggagtagac gatcaggatg ggggccttta gcgcatcaac atagcttgac
     1921 ggcgacaaca ctctggcgag cgcggtgtgt ggctccggcg cagagcattt gtggtagtcc
     1981 gggacgggtg cgccgtctgt cagttcgacg cccaggtcgg tccagtccca cactcccatt
     2041 agggagatga ctgccttgta ggtatccgga tggactgccg cctggtagaa cgatatgaac
     2101 ccgccatagg agtgccccat gatgccgatg cacttaggat ccgcgattcc gttctctacg
     2161 gcccactgaa cgccttcgca aacatcctgc tgcgccagga gcatgttctg gcttgcggta
     2221 tccgtgtact cgttgccata tccgctagag aagcggaagt ctaccgacag caccgctatc
     2281 cccaggtttg atgcgagcag cgcgtgctca ttcggggccc acgtgaacct ggcctggggc
     2341 ccaccatgga cgttgatcaa gagaggtagg ttgcgcgggc tcttgccgag cggcactgtc
     2401 aggtatgcaa agaccgtcgc tccgtcgctt gcgctgaacg acaccggata ggtgtgcccg
     2461 acatcctcta tcggtatgtc ggacgacagg agaagagtcg ccgtccctgt cttcacattg
     2521 taaagccgat agctgccgta gtctctgtcg tctctatgca taagcacaac gtagtcgaac
     2581 tccgggctta tcgccaccgg gaagtggttc tcacccaggt cccgaaggac attatcagct
     2641 a
//
LOCUS       DAOTAC010000318         2612 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_30511_length_2612_cov_4.635119, whole genome shotgun sequence.
ACCESSION   DAOTAC010000318 DAOTAC010000000
VERSION     DAOTAC010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30511_length_2612_cov_4.635119"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="PLM74_10930"
     CDS             complement(<1..419)
                     /locus_tag="PLM74_10930"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HON43622.1"
                     /translation="MGMESERLFIGIDLGGTKIASALVDADMRMIDRVRVPTRPEEGP
                     SAVIGRMLDTVRDLTARKGCDVSDVRAIGIGCPGPLDQRTGIVLDAPNLMWKDVSLAA
                     PIARATGRPVYLENDANVAALAENRLGAGRGAKHMMYV"
     gene            complement(497..1597)
                     /gene="ugpC"
                     /locus_tag="PLM74_10935"
     CDS             complement(497..1597)
                     /gene="ugpC"
                     /locus_tag="PLM74_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HON43623.1"
                     /translation="MAGVKLEHVYKKFGNVVAVNDANLEIRDKEFLVLVGPSGCGKST
                     TLRMVAGLEEITSGDIYIGDTLVNDIPPKDRDIAMVFQNYALYPHMDVYNNMAFGLKL
                     RKFPKAEIDARVKDAARILGIENLLDRKPKALSGGQRQRVAVGRAIVREPKVFLMDEP
                     LSNLDAKLRVQMRAELSKLHNRLQTTIIYVTHDQTEAMTMGDRIVVMKDGFIKQVGAP
                     LEIYERPDNMFVAGFIGSPAMNFAPGVLRKEGDDYIVDCESFRIGVPRGRFEAIEKYV
                     DEKVILGIRPENIEDIDFIADAPEDRVVKCMVDVIEPMGAEAYLYLTTGPHSFTARVN
                     PRTNARDGEPHSVVLNMDECHLFDPSTENAIR"
     gene            1749..>2612
                     /locus_tag="PLM74_10940"
     CDS             1749..>2612
                     /locus_tag="PLM74_10940"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HON43624.1"
                     /translation="MPECRPLVLMRGMTVVPVGNVQEQLAFKGFPLGEVDNVFGIRTE
                     LAVRQFQASVGLPETGIVDAETWRLLFDGLPLPGDACFPDDPPDMATADPLGPANPDA
                     NISSRQATQTRQPLSIQISLAQRRLALLRGGVEAAHYPVAVGKPATPTPSGSWTVLNK
                     AFNPGGVFGTRWMAFTRRGHGIHGTNQPQSIGHAVSNGCVRMYNADVESLFDQVQVGV
                     PVLITTGVTAPAPTPGTYVVRAGDTLFLIAQRFGTTVDAIRRANNLTTDILAVGQVLV
                     IPGAVARPTTTG"
BASE COUNT          501 a          809 c          725 g          577 t
ORIGIN      
        1 acatacatca tgtgtttggc gcctcttcct gcgccaagtc tgttctctgc aagcgccgcg
       61 acattggcgt cgttctcgag atatacaggc ctcccggtag ccctggctat aggcgccgca
      121 agggagacat ctttccacat cagattgggt gcgtccagca ctatgccggt tctctggtcc
      181 aggggtcccg ggcagccgat ccctatggcc cggacatcgc tcacatcgca cccctttctt
      241 gcggtcaggt cccgcaccgt gtcgagcatc ctgcctatta cggcgctcgg cccttcctcc
      301 ggcctggtgg ggaccctgac gcggtcgatc atcctcatat ccgcgtcgac caacgcagaa
      361 gctatcttcg tgcccccgag atcaatgccg atgaagaggc gctcgctttc catgcccata
      421 cactaccgcc cccgttccac gatggctgta caacggcacg ccggggcatt acgccccggc
      481 gatgtattgg tatgccttat ctgatggcat tctcagtaga tggatcgaac aggtggcact
      541 cgtccatgtt gagtacgact gaatgaggct cgccgtctcg agcattggtg cgagggttga
      601 ccctggccgt aaatgagtga gggccagtcg tcaggtaaag ataggcctcg gcgcccatgg
      661 gctcgatcac atccaccatg cactttacta ccctatcctc aggcgcgtcc gcgatgaagt
      721 cgatatcctc gatgttctcg ggcctgatgc ccaagataac cttctcatcg acgtacttct
      781 ctatcgcctc aaacctgccg cgcggcactc cgatgcggaa gctctcgcaa tccacgatat
      841 agtcgtcgcc ttccttgcgt aggacgcccg gcgcaaagtt cattgcaggg ctgccgataa
      901 agccggccac gaacatgtta tccggccgct catagatctc aagaggcgct cccacctgct
      961 tgatgaagcc gtctttcata accacgatgc ggtcacccat ggtcatggcc tcggtctggt
     1021 cgtgggtgac gtagattatc gtcgtctgca gcctgttgtg gagcttcgac agctctgccc
     1081 tcatctgaac cctcagtttg gcgtccaggt tggacagggg ttcgtccata aggaacacct
     1141 taggctcgcg cacgatcgcg cgccctactg caacgcgctg cctctggcca cccgacaggg
     1201 ccttgggctt acggtcaaga aggttctcga tgcccagaat ccgagcggca tccttcactc
     1261 tggcgtcgat ctctgctttg gggaacttcc gcagcttgag accgaaagcc atgttgttgt
     1321 agacatccat gtgggggtac agtgcgtagt tttggaaaac catcgcgatg tcgcggtctt
     1381 ttggcggaat gtcgttgacc agagtgtcgc cgatatagat gtcgcctgaa gtgatctcct
     1441 caagcccggc caccatccgg agagttgtcg acttcccgca tcccgaaggg cccaccaaca
     1501 ctaggaactc cttgtcgcgg atctccaggt tagcatcgtt taccgcgacc acattgccga
     1561 acttcttgta cacatgctcc agttttactc ctgccatgtg acgtcctccc actgctttat
     1621 attaatcggc ggcatctttc gcccaccggt tcaactacct ttattcggcg gtgcaataaa
     1681 gaatccttca acggatttac gccgctgcgc gtataatggt gcagaaagca gacaggtggt
     1741 gatcgcaatt gcctgaatgc aggccactgg tgctgatgcg gggaatgact gtggttcccg
     1801 tgggcaatgt tcaggaacag ctcgcgttca aaggtttccc gttgggcgaa gtggacaacg
     1861 tattcgggat ccgcaccgag cttgccgtac ggcagttcca ggcaagtgtg ggtcttcctg
     1921 aaaccggcat tgttgacgcc gagacatggc gcttgctctt cgatggcctt ccgctgcctg
     1981 gggatgcatg tttccccgat gatcccccgg atatggcgac cgccgatccc ttaggcccgg
     2041 cgaaccctga cgcgaacatc tcttccaggc aggcgactca gacccgccag ccgctctcga
     2101 tacagataag cctggctcag cgtcgcctgg cactgctcag gggcggagtc gaagccgcgc
     2161 actaccctgt tgcagtgggc aagccggcca ctccgactcc gtcgggatcc tggaccgtgc
     2221 tgaacaaggc gttcaacccc ggcggcgttt tcggcactcg ctggatggcg ttcaccaggc
     2281 gcgggcacgg aatacacggc accaatcagc ctcagtctat cgggcacgca gtatccaacg
     2341 ggtgtgtgag gatgtacaat gctgacgttg agtcattgtt cgaccaggtg caggtgggag
     2401 tgcctgtatt gataacaacg ggcgtcaccg cccctgctcc cacgccggga acatacgtgg
     2461 tgagagccgg cgacacgttg ttcctcatcg cgcagaggtt cgggaccaca gtggacgcca
     2521 tccggcgcgc caataacctg acaacagaca tactggctgt cggccaggta ctagtgatcc
     2581 ctggagcagt tgcgcgcccc acgaccaccg gc
//
LOCUS       DAOTAC010000319         2607 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_30612_length_2607_cov_5.014107, whole genome shotgun sequence.
ACCESSION   DAOTAC010000319 DAOTAC010000000
VERSION     DAOTAC010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30612_length_2607_cov_5.014107"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="PLM74_10945"
     CDS             <1..840
                     /locus_tag="PLM74_10945"
                     /inference="COORDINATES: protein motif:HMM:NF019758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43625.1"
                     /translation="HLNQAGCPLYASGLNARVADLRLQLKRTARRRRSKEKRADIRSQ
                     LEAVQAELGSLQTQKCGAAQKRACRKLLARRGVIVGRMQSLYERMRRLGSEERFVHEF
                     RRKQALLERLGYMKDGKLLARGEIARELHTQELLVTELVFSGVFHEFTEDQIASLAVC
                     IDYEPRRGEFPAKSVPFDVDRIREIAAQVEMVEESYVGTSTVRFFTGLAPLAYRWSQG
                     AEFADMLRGSAYAEGDVVSAFRRSIDLLRQVRAACKADAALVDKISDVMRRIDRDVVE
                     VVL"
     gene            complement(797..1972)
                     /locus_tag="PLM74_10950"
     CDS             complement(797..1972)
                     /locus_tag="PLM74_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PRK06851 family protein"
                     /protein_id="HON43626.1"
                     /translation="MAKIARALARRMFAGGNTAEGFRSFYNHIIGQDAARVIVIKGGP
                     GVGKSTFMKHIAEHITNLGQSVDFFHCSSDPGSLDGVVFPQIGVALIDGTAPHIVDPR
                     FPGAVDEILYLGDYWNESAIRASRNDIVACTKNISRLFQRAYRFLRTAGDVLDDWSEA
                     NAEALMTGVANKIARSVVDQIAGDAPVADCPGAERHLFASAITPDGFVNYLDTVVSGC
                     DRKYIVRGGPGSGKSTLISRVAARAVECGMDVELFHCSLNPASLEHLVIPSIGTAIIT
                     SVEPHEWTASPDSAVHDVIIDMEQCLDPATVRRNQDIIEYDRKLMHEAMSRAVHFLGE
                     ARRAHHFLESFYTPNIDFDAVGELREKTLRRILATAPPPRPTPPTEPPRPHRGRSAS"
     gene            2113..>2607
                     /locus_tag="PLM74_10955"
     CDS             2113..>2607
                     /locus_tag="PLM74_10955"
                     /inference="COORDINATES: protein motif:HMM:NF039187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HON43627.1"
                     /translation="MGDRRQKDFVGPIILIVLGVLLLLNTTGVVPWEAWESLWRFWPA
                     LLILWGISIIFGRGSPAGRILVLALLALFIVFAVSNIASESWWGWGRRVPDGGATRGA
                     LPYSKTLTVSSDQFSPSRIRLSADIGAGKVFIGTQSGGDILKAVASYSRASRAPRLDT
                     SMAGS"
BASE COUNT          479 a          751 c          864 g          513 t
ORIGIN      
        1 catctgaacc aggcaggatg ccctctgtat gccagcgggc tcaatgctcg ggtcgccgac
       61 ctcaggttgc agctgaagcg gacggcccgg cggcggcgca gcaaggagaa gcgggccgat
      121 atccggtcac agctggaggc ggtccaggcc gagctgggct cgcttcaaac ccagaagtgt
      181 ggcgcggcgc agaagagggc atgccgtaag ctccttgcca ggcgcggagt aatcgtcggg
      241 cggatgcaga gcctgtacga gcgcatgcgc cgcctaggca gcgaagagcg gttcgtccac
      301 gagttccggc gcaagcaggc cctgctggaa cgactgggct acatgaaaga cggcaagctc
      361 cttgcgcgcg gcgagattgc gcgcgagctc catactcagg agctgctcgt cacagagctg
      421 gtattttcgg gcgtgttcca cgagtttacc gaagaccaga tagcgtcgct cgcggtatgt
      481 atcgactatg agccgcgccg gggagagttc ccggctaagt cggtgccttt cgatgtagac
      541 aggatcaggg agatcgcagc ccaggtggag atggtggagg aaagctacgt gggcacaagc
      601 acggtgcggt tcttcaccgg gctggcccca ctcgcgtacc ggtggagcca gggcgctgag
      661 ttcgccgata tgctgcgcgg ctcggcctat gccgagggag atgtagtgtc ggccttccgg
      721 cgcagcatcg acctgctgcg gcaggtgcgc gctgcgtgca aggccgatgc cgctctggtc
      781 gacaagattt cggatgttat gaggcggatc gaccgcgatg tggtcgaggt ggttctgtag
      841 gcggggtggg ccgagggggc ggagcggttg cgagtatccg tcggagcgtc ttctctctca
      901 gttcgcccac ggcgtcgaaa tcgatgttcg gggtgtagaa cgactccagg aaatgatggg
      961 cgcgccgagc ctcgcccagg aagtgcactg cccttgacat cgcttcatgc attagcttgc
     1021 ggtcgtactc gatgatgtcc tggttacgtc gcaccgtggc cgggtccagg cactgctcca
     1081 tgtcgatgat cacgtcatgg acggcgctgt cgggcgaggc cgtccactcg tgtggctcta
     1141 cggatgtgat tatcgcagtc ccgatagacg ggatcaccag gtgctccagg ctcgccgggt
     1201 tgagggagca gtggaacaac tccacgtcca tgccgcattc cacagcgcgg gctgccacac
     1261 gggagatcag ggtggacttg cccgaacccg gtcctcctcg cacaatgtac ttgcggtcac
     1321 agccggatac cacggtatcg aggtagttga cgaagccgtc gggagtgatc gccgacgcga
     1381 acagatggcg ctcggccccg gggcagtcgg cgaccggcgc atctcctgct atctggtcca
     1441 cgaccgaccg ggcgatcttg ttggcgactc cggtcatgag ggcctcagca ttggcctcgc
     1501 tccagtcgtc aagcacatcg cctgcagtgc gcaaaaaccg gtatgccctt tggaaaagcc
     1561 gggatatgtt cttggtacat gcgactatgt cattgcgaga agcgcggatt gcggattcgt
     1621 tccagtagtc cccgagatac aggatctcgt ctacagcgcc tgggaaacgg ggatcgacta
     1681 tgtgaggtgc cgtgccgtcg atcagggcca cgccgatttg gggaaagact accccgtcga
     1741 gcgagcccgg gtcggacgag caatggaaga aatccacact ctgcccgagg ttcgtaatgt
     1801 gctcggcgat gtgtttcatg aacgtcgact tgcctacgcc cgggccgcct ttgatcacga
     1861 ttacccgcgc ggcgtcctgg ccgatgatat ggttgtagaa cgagcgaaac ccttcggcgg
     1921 tgtttccgcc ggcgaacata cgccttgcga gagctcgggc gatcttggcc atgctgtcgc
     1981 ctccatatca gtcatgttct ttcggcatcc cacctaaact atgcggcaaa cacttcaagc
     2041 ggcacgacgc gcgtgaagga atctgcgccg cgtatgcgaa ttaccgacgg agagagcgag
     2101 gaggcgatat ccatgggcga ccgtaggcag aaggacttcg ttggaccgat catcctcatt
     2161 gtcctcggcg tattgctact cctgaacacc accggagtgg tgccgtggga ggcatgggag
     2221 tctctctggc gattctggcc tgcactgctc attctgtggg gcatcagcat tatctttggg
     2281 cgcggaagtc cggccggtcg tattctagtg ctggccttgc tggcgctgtt catagtgttc
     2341 gccgtatcga acattgcatc cgagtcatgg tggggttggg gccggcgcgt gcccgacggc
     2401 ggggcgaccc ggggcgcttt gccttactcc aagaccctga cggtgagctc tgaccagttc
     2461 tccccgagcc gaattcgcct tagcgccgac ataggcgccg gaaaggtatt catcggcaca
     2521 cagagcggcg gcgacatact caaagcggtc gcgtcgtatt cacgtgcatc ccgtgcgccc
     2581 aggctcgata catcgatggc gggctcg
//
LOCUS       DAOTAC010000320         2580 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_30987_length_2580_cov_4.315644, whole genome shotgun sequence.
ACCESSION   DAOTAC010000320 DAOTAC010000000
VERSION     DAOTAC010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30987_length_2580_cov_4.315644"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="PLM74_10960"
     CDS             <1..562
                     /locus_tag="PLM74_10960"
                     /inference="COORDINATES: protein motif:HMM:NF036773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase-related protein"
                     /protein_id="HON43628.1"
                     /translation="LRCVEWLSRDDLTTRCGHAGPAVHTPGAQCRGSHVFEYAVVPHS
                     GTWVAGGTAQIAREYLSPPVCFMASVHPGGISEDVSLLEFASGPALITAVKKAEADDA
                     LVVRLHNPTSDEADATLLSGVPVASAAMARLDETPGEALQVERGFFPAANPDAAGEPD
                     RCRIRVRLRPKQIATVLLYPAKDTLG"
     gene            complement(569..1381)
                     /locus_tag="PLM74_10965"
     CDS             complement(569..1381)
                     /locus_tag="PLM74_10965"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HON43629.1"
                     /translation="MSNYKLLVTDIDGTLVDSKQEVPEENHRAVQRLLAAGALFTFAT
                     GRIEESTIPYARDLKINAPAILYNGAKIVDLERGEVLFERNLPMDQAIAALEVASEFD
                     VRTHVYIDGKVYVARIDSVVRDYAKKDRVDCTEIGDLVTFLKSGPTHVPPTKFLIIGE
                     SESLGILRRRIHAVFPEGALVESDACYLELLPPGVSKGAALSVLCEILGLDPTDTVAI
                     GDNMNDVEMIQVAGMGVAVENAHPYAKEQAAFVAPSNEECGVATVIRRFFGI"
     gene            complement(2025..2222)
                     /locus_tag="PLM74_10970"
     CDS             complement(2025..2222)
                     /locus_tag="PLM74_10970"
                     /inference="COORDINATES: protein motif:HMM:NF019998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HON43630.1"
                     /translation="MERPQDDEPYYAIEGQITDQMYLGSRRRYKIQTPEGHLLLVDSE
                     REWPTGADIRAWVRHDKVHVY"
     gene            complement(2223..2580)
                     /locus_tag="PLM74_10975"
                     /pseudo
     CDS             complement(2223..2580)
                     /locus_tag="PLM74_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014629407.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
BASE COUNT          529 a          733 c          758 g          560 t
ORIGIN      
        1 gcttcgctgc gtggaatggc tgtctcggga tgatctcacc acaaggtgcg gccacgcggg
       61 ccctgccgtt catactcccg gggcgcagtg ccggggaagt cacgtattcg agtatgcggt
      121 cgttccgcat tcaggtacgt gggttgccgg cggcacggcg cagatcgccc gcgagtatct
      181 gtcgcctccg gtatgcttca tggcatctgt tcatcccggc ggcatctcgg aggatgtatc
      241 tctcctagaa tttgcgagtg gccccgcgct gataaccgcc gtgaagaagg ctgaggccga
      301 cgacgccttg gtggttaggc tccacaaccc gacctcagat gaggcggacg cgacactctt
      361 gtcaggagtg cctgtggcaa gtgcggcaat ggcgagactg gatgagacgc cgggcgaggc
      421 ccttcaggtc gaacgcgggt tctttcccgc cgccaatccc gacgcagcgg gggagccgga
      481 ccggtgcaga atccgggtcc ggttgaggcc gaagcagata gccactgtcc ttctctaccc
      541 ggccaaggat acgctcggat agcagatgtc agatgccgaa gaaacggcgt atgacggttg
      601 caacgccaca ctcctcattg gacggagcta cgaacgcagc ctgctccttg gcgtacggat
      661 gcgcgttctc gactgcgacg cccatcccgg ctacctgaat catctcgaca tcgttcatat
      721 tgtcgcctat agccacagtg tcggtggggt ccaggcccag aatttcgcag aggacagaga
      781 gggcagcgcc cttggacaca cccgggggca gtagttcaag atagcaagca tcggattcta
      841 ctagcgcccc ttcggggaac accgcgtgta ttcggcgacg cagtatccca agggactcgg
      901 actcgccgat gatcaggaac ttagtaggtg gcacgtgagt gggcccgctc ttgagaaagg
      961 tcactaggtc gcctatttcg gtacagtcta cacggtcttt ctttgcataa tcccttacca
     1021 ccgagtcgat tctagcaacg tacactttgc cgtctatata cacgtgtgtt ctgacatcaa
     1081 actcggatgc gacctccaaa gctgcgatgg cttgatccat gggcaggttt cgctcaaaga
     1141 gtacctcgcc ccgctcgagg tcgacgatct tcgcgccatt gtacagtatc gcgggagcat
     1201 ttatcttgag gtcgcgggca tagggaatcg tcgactcttc gatcctacca gttgcgaatg
     1261 taaagagagc gcctgcggcc aggagtcgct gcacagcccg gtggttttct tcggggacct
     1321 cttgcttcga gtctaccaaa gtgccgtcga tatccgttac gagcagcttg tagttggaca
     1381 taatcacacc gcctcacgtc ctgattcggc atcttcggga attaccctgc ataagggacc
     1441 cctgttcggc tctcagacgg cgcgcaccga tgaaactgcg acgttagcac tcagcagtac
     1501 tgtagcacgg ggacctgcct gcgctcggag acgccagttg ctaatgaaag cgacgctgcg
     1561 caggcctttg ggcaaaaccg cgtttagcag ggagttagcg aaccggtaac aatactgtac
     1621 ggtttgtacg ccaacctgct caggatgtgc ccggttttga ccgtgagcag gcaaaacacg
     1681 ggagtttttg gcgtttcatg gagaatttgc ggccgcctaa ttccttctcg ggcatgctcg
     1741 atggaaagag gcgcatggcc agattatgcc agactcctct gcctgcggat tcaacgacgg
     1801 cgtaatgaca gaaaaaggag gtgtaattgc agacccgaat tcagacaatt gtcgcctgcc
     1861 aaaccaggca ggatctgaac agggtgctcg aaaaacctat tcagaaacgg gcaggatgcc
     1921 ccagaaccca atcggggccc accacaggag aacgctgcgc ccaggaatat ccataggcac
     1981 actgcgttca tagggccggc gatagcggga atctcggccg tgcatcagta cacgtgcact
     2041 ttgtcgtgcc taacccatgc cctgatatcg gctccggtgg gccattcgcg ctctgaatcc
     2101 acaaggagca ggtggccctc gggtgtctga atcttgtacc tgcgtcgcga acccaggtac
     2161 atctggtcgg ttatttggcc ctcgatcgca tagtaaggtt cgtcatcctg cgggcgttcc
     2221 acgaccagtt ccacttcatc agcagagagg gcctcctcct ggtcgtgggt tatgtatatg
     2281 gtggttatgt tgagccgcct ctgcagtttc tttatctcga acgtagtcta tagcggattt
     2341 tggcatccag gttggatagg ggttcatcga ggaggaggat ctcgggatcg actacgagcg
     2401 cccgcgccag ggccactctc tgctgttggc caccggatag ctgacgcggg tagcgctttt
     2461 ccaagcccgg aaggtcaacg aggttgaggg cccactccac cttattgcgt actgcttccg
     2521 gctgcatcgt cctgagccta ggtccaaagg agatgttctc catgaccgtc atgtgaggcc
//
LOCUS       DAOTAC010000321         2570 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_31145_length_2570_cov_4.027435, whole genome shotgun sequence.
ACCESSION   DAOTAC010000321 DAOTAC010000000
VERSION     DAOTAC010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31145_length_2570_cov_4.027435"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="PLM74_10980"
     CDS             <1..666
                     /locus_tag="PLM74_10980"
                     /inference="COORDINATES: protein motif:HMM:NF013012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HON43631.1"
                     /translation="RLDPPVLCLTVSGGHTELVAMDGHGHYEVLGRTRADAAGEAFDK
                     VARVMGLPYPGGPELEKLARSGDPDAVEFPRAHVEGRPFDFSFSGLKTAVINYLHTEE
                     QRGVRIDYPDVAAGFQKAVVYELVSRIEPAVRATGIRRLAVSGGVAANGALRAAMDKL
                     AADLEIEVFCPPLPLCTDNAAMIASAGYYGLVRGQLSGLDANAAPGARLEDAQAEGYG
                     SLP"
     gene            713..1594
                     /locus_tag="PLM74_10985"
     CDS             713..1594
                     /locus_tag="PLM74_10985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HON43632.1"
                     /translation="MSHISDGSDNARKPRRWRGFVVAVVILAVIAGALLSAALWYVRF
                     PCEGSYFSILLVGEDRDYARNGEAIARAGRMDAIMLLIVPRRGKALLVSIPRDSLVSF
                     PGGGRRRINSAAATGGMDLARQVVSELVGLDVHRHASVNFAGFIEIIDAVGGVDITVD
                     RRMKYTDRAGGYSIDLVPGTYHMDGEMALSFVRYRHDALGDISRTARQQQFFRALLDE
                     LASWRGIRSIKKIMTIAQDNTNTDLSVREMAAVGWRLRRLNSNALESTTLPGHFKGAY
                     WEPDYEAIHALVEPLGR"
     gene            1639..2382
                     /locus_tag="PLM74_10990"
     CDS             1639..2382
                     /locus_tag="PLM74_10990"
                     /inference="COORDINATES: protein motif:HMM:NF015962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HON43633.1"
                     /translation="MRVDVSLIPGEVAAKDVSNAAVVVIDVLRASSTVATVLANSCEE
                     VIPAESIEEAITIASGYARPDYVLGGERRGLAIDGFDLGNSPAEYSEARVAGRKVILA
                     TTNGTRAVKQYGGAREIVVACFLNAGAVLHMLATAEDRDVLLLCAGQDGRFSIEDAFC
                     AGLLASELAKTTGTSLSDSARAAAAIYEHRRGSIEEALIESEHGAYLAGIGFADDVRY
                     CARQSVLDVVPKVYDRRVIRRARLGSRES"
BASE COUNT          507 a          660 c          876 g          527 t
ORIGIN      
        1 cggctggatc caccggtgtt gtgcctgact gtgtccgggg gccatactga gctcgtggcg
       61 atggacgggc acggacacta cgaggtgctt ggacgtaccc gcgccgatgc ggccggagag
      121 gctttcgaca aggttgccag ggtaatgggg cttccttatc cgggcggccc tgagctggag
      181 aagctggcac ggtcgggcga cccagacgca gtggagtttc cccgggccca tgtggagggc
      241 aggccctttg atttcagctt cagcgggctc aagacagccg tcatcaacta cttacatacg
      301 gaggagcaga gaggcgtccg aatcgactat ccggacgtgg ctgccggatt ccagaaagcg
      361 gtcgtgtatg agctcgtgtc gaggatcgaa ccggcggtgc gcgcgaccgg cattcgcagg
      421 ctcgcggtat cgggcggagt ggcggccaac ggggcgctca gggcagccat ggacaagctt
      481 gcggccgatt tggaaatcga ggtgttttgc cctccgctgc cgctgtgcac tgacaatgcc
      541 gccatgatag cttcggctgg ttactacggc ctggtccgcg ggcagctctc ggggctcgac
      601 gctaacgcag cgccgggggc gaggctcgaa gatgctcaag cggaaggata cggcagttta
      661 ccgtagaatt acactgaatg tggacgcaca aggcgtctga ggagtatgca ttatgtcgca
      721 catttccgat ggatcggaca atgctcgaaa gccgcgtcgc tggcgcgggt tcgtagttgc
      781 agtagtgatc ctcgctgtca tcgccggagc gctgcttagc gcggcgctgt ggtatgttcg
      841 ctttccttgc gagggttcgt acttcagcat attgctggtg ggcgaagatc gcgactacgc
      901 aagaaacggg gaagccatcg cccgagcggg caggatggat gccataatgc tactcattgt
      961 tccgcggcgt gggaaggcgc ttctcgtctc gattccccgt gacagcctgg ttagcttccc
     1021 cggaggtggt cgacgcagga taaactccgc cgcggccaca ggcgggatgg atcttgcacg
     1081 ccaggtcgtg tctgagcttg ttgggttgga cgtgcatagg catgcgtcag tcaacttcgc
     1141 ggggttcatc gagatcatag acgcagtggg aggggtcgac ataaccgttg accggcggat
     1201 gaaatatacc gatcgcgccg gaggatactc tatcgacctt gtgcccggaa cgtaccatat
     1261 ggacggcgag atggctctca gcttcgttag gtaccgacac gatgctctgg gagacatttc
     1321 gcgcacagct cgtcagcagc agttcttccg ggcgctcttg gatgagctgg cgagttggcg
     1381 gggaattcgg tctattaaga agatcatgac aatcgcccag gacaacacca atacggactt
     1441 gtctgtgcgc gagatggctg ccgtgggatg gcgtttgcgc aggctcaatt cgaacgctct
     1501 tgagtcgaca acgcttcccg gccattttaa gggcgcatac tgggagccgg attatgaagc
     1561 catacatgcg ctggtagagc cgctgggtcg gtgatgtatc ctagctcata gctcaagcta
     1621 gctgaaaggg gagccgcaat gcgagtcgac gtatccctaa ttcccggtga agttgccgcg
     1681 aaagatgtat caaacgctgc agtagtcgta atcgacgtcc tgagggccag cagtacagtt
     1741 gccacagtgt tggcgaattc gtgcgaggaa gtcatcccgg cggagagcat agaagaggcc
     1801 ataacaattg ccagcggcta tgccaggccc gactacgtgc taggaggaga gcggcgcggg
     1861 cttgcgatcg acgggtttga cctcggcaat tcgcctgccg agtactccga ggcgagagtg
     1921 gccgggagga aagtgatctt ggcaactacc aacggcactc gcgctgtgaa gcagtacggg
     1981 ggcgcccggg agatagtcgt agcctgcttc ctcaacgctg gggctgtgct tcacatgctc
     2041 gcgactgcgg aagatcgtga tgtgctgctg ctttgcgcag gccaggacgg acgtttcagc
     2101 attgaggacg ccttttgtgc agggctattg gcatcagagt tggctaagac gacggggacg
     2161 tcgctttccg actcggctcg cgctgccgcg gcgatctacg agcatcgcag aggttccatt
     2221 gaagaagcgc tgatagagtc ggagcatggt gcgtatcttg ccgggatcgg gtttgccgat
     2281 gatgttaggt actgcgctcg ccagtcagtg ctggatgtgg tcccgaaggt ctatgatcgg
     2341 agggtcataa gaagggctcg tttggggtca agggaatcat gaacctcgtg ccttgggccg
     2401 ggggttggag gagtggttga tttgctgacg gacatcgaga agaacgggat cgtcaaatgg
     2461 gaagacgtcc aggcctctcg gggatgcccc ggcgagggcc gggcgaagaa gggtccagtg
     2521 gcggtcatcg agtgcctgca ggagattcca tgtaacccgt gcgaaaccgg
//
LOCUS       DAOTAC010000322         2558 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_31341_length_2558_cov_4.121854, whole genome shotgun sequence.
ACCESSION   DAOTAC010000322 DAOTAC010000000
VERSION     DAOTAC010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31341_length_2558_cov_4.121854"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..334)
                     /locus_tag="PLM74_10995"
     CDS             complement(137..334)
                     /locus_tag="PLM74_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43634.1"
                     /translation="MLLIGMAFIIITALGTSLAGRSTSRLAGTAGTWPILGASTLGPT
                     ARGFRIEVFGRSVRIEIEAAK"
     gene            complement(444..1799)
                     /locus_tag="PLM74_11000"
     CDS             complement(444..1799)
                     /locus_tag="PLM74_11000"
                     /inference="COORDINATES: protein motif:HMM:NF019097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HON43635.1"
                     /translation="MYLAMCLVAVGLAVRTRMNASQDAVTATSSGQPGMGDAAGQAER
                     VHRTLRETVRRSLERFLGLFRARSGNTGALPATSESDRFSSFGSRLLKAVLLSMGGLD
                     PEDPRTFLRAELPMILSAQRDADLPSRSDSSRRGTPTPTADSSSGTISGTLSEYEELT
                     QDPPEAVTAVWGSVSTVEASTAAGSSAASSPAAGSPAAGDPLPVPTSAQAVSPAPSAP
                     SATSAQPRSSNSTTPTRLAARAVSDTLVLRGSAPKVAVIHTHSSEAYRPTSGADYCWG
                     KPDGVITVGAALVEELSGKLGVPTVHSSQIHDYPSWTKSYSAAHDTMKSLIKSYPGID
                     VVLDIHRDSLPVSKSKLKTATVNGEKVARVLFVVTDDSSGLAHPNWRKNYEFALRLSA
                     QVEAMYPGVTRGVAIHKHARFNQHLHDRAIIVEIGGTSNTIEEANRAARLVARALARV
                     I"
     gene            complement(1995..>2558)
                     /gene="gpr"
                     /locus_tag="PLM74_11005"
     CDS             complement(1995..>2558)
                     /gene="gpr"
                     /locus_tag="PLM74_11005"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: protein motif:HMM:TIGR01441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HON43636.1"
                     /translation="GLKSVAAISPGVLGLTGIETAEVILGVVGRVRPNLVIVVDALAA
                     RSVSRLGTTIQLGDTGIQPGSGVGNRRFGITRETLGVPVIAVGVPTVVHAMTIVADAL
                     SIMGQPPQSGDREQQGGPRSIQEVMGAPGLPPAVSQMLQPYLGTLIITPKEVDVLAKE
                     LSDVVAGGINYALHPAIDEEEIYQYLQ"
BASE COUNT          457 a          808 c          792 g          501 t
ORIGIN      
        1 acttgccgtg gtctatgtgg gctatgatac agaaattcct gatagactgg atatctatgt
       61 ccatgcacac ctcagcgtcc gtgagaatcg ggggtatccc aagtcgcatt ataccacacc
      121 tcttgggcag gaacggctac ttggcggcct cgatctcaat ccgaaccgag cgaccgaata
      181 cttctatccg aaatccacgg gctgtggggc cgagcgtaga tgcgcccaaa atcggccacg
      241 tgccggcggt gccggctagg cgcgaagtgg atcgacccgc gaggcttgtc ccaagcgcgg
      301 tgattatgat gaacgccatg ccaatcagca acatcgctgc aaccccgaag gcgtcggtgc
      361 gcctgcgccg gcgggcgcgt cgaggacctc gcgggcgttc gcggcgttca gagcgttcac
      421 agtgttcaga gcgttcgggg cgttcagatg actcgagcaa gggcccgggc gacgagtcgg
      481 gccgccctgt ttgcctcttc gatggtattg ctggtccctc ctatctccac tattattgcc
      541 ctatcgtgca agtgctggtt gaaacgcgcg tgtttgtgga tggcaactcc ccgggtgact
      601 cccgggtaca tcgcctcaac ttgggcggag agtctcagcg caaactcgta gttcttcctc
      661 cagttcgggt gtgcaaggcc tgagctgtcg tcagtcacta cgaagagcac tctggctact
      721 ttctctccgt tcactgtcgc ggttttcagc ttactcttgc tgacaggcag cgaatctcgg
      781 tggatgtcta gcacgacatc gatgcctgga tatgatttga tcaaagactt catggtatcg
      841 tgcgcagccg aatagctctt cgtccagctg gggtaatcgt ggatctgtga ggagtggacc
      901 gttggcactc caagtttccc cgaaagctcc tctacgagcg ctgcgccaac cgtgatgact
      961 ccatccggct tgccccagca gtagtcggcg cctgatgtcg gcctgtatgc ctcggagctg
     1021 tgcgtgtgga tgactgccac cttcggagct gatcccctga gaaccagcgt gtctgaaacc
     1081 gcgcgcgcgg caagacgcgt cggcgtcgtc gagttgctcg accggggttg cgctgaggtc
     1141 gcggacggtg ctgacggcgc agggctgaca gcctgagcag aggtgggaac aggcaacgga
     1201 tcgcctgccg ccggggagcc cgcagctggg gagcttgccg ccgaggaacc cgcagccgta
     1261 ctggcctcga ctgtggacac actgccccac accgcagtga cggcctcagg cgggtcctgc
     1321 gtcagctcct cgtactccga caacgtgccc gatatcgtgc cggaggacga atcggcggtg
     1381 ggcgtaggtg tgcccctacg cgagctgtcg gagcgcgacg gcagatccgc gtcgcgctgc
     1441 gcgctgagga tcatggggag ctcggcacgg agaaaggtgc ggggatcctc gggatcgagc
     1501 ccgcccatgg agagcagcac tgccttgagc aggcgcgagc cgaaggaact gaagcggtcg
     1561 ctctcggacg ttgcaggcag ggcgcccgtg ttgcctgacc tcgctctaaa cagaccgaga
     1621 aaccgctcca gcgaccggcg cacagtctcg cgcagggtcc ggtggacgcg ctcagcctgc
     1681 ccggcagcgt ctcccattcc gggttggccg gaagacgtag cggtcacggc gtcctgcgag
     1741 gcgttcatcc gggtacgcac tgccaggccc actgctacaa gacacatcgc caagtacaag
     1801 ataaagagat tcccgagcgg gccggctccg cgtgccgcag cgcccctctc tcgccggcgt
     1861 tttgtctccc ttgaggccct gcgccccgcc tttcgcgcgg tccggagcaa agagaccacc
     1921 ccttctcttt gcacctgtat tccgcgggca tgcgcggtat tcgctgcggg gcatttccca
     1981 ttggaacagg ctcgctactg caaatactgg tatatctcct cctcgtctat ggccggatgc
     2041 agcgcatagt tgatcccgcc ggcaaccacg tcagacagct ccttggccag gacatccact
     2101 tctttggggg ttatgatcag cgtgcctagg tagggttgaa gcatctggct caccgcagga
     2161 ggcaaccccg gagcgcccat cacttcctga atgctgcgag gtccgccctg ctgctcacgg
     2221 tctccactct gcggaggttg ccccatgatg ctgagagcat ccgcgacgat agtcatggcg
     2281 tgcactacgg tggggacgcc cacggcgatc acgggaacgc cgagggtctc cctggttatc
     2341 ccgaacctcc tgtttcctac gcctgatccc ggctggatcc cggtgtcgcc cagctgaatc
     2401 gttgtcccga gcctggacac gctgcgcgcc gcaagagcgt caaccacaat cacgaggttg
     2461 gggcggacgc gtcccacaac gcccagaatg acctcggcgg tctctatacc agttagccca
     2521 aggactcccg gggatatggc tgccaccgac ttcagccc
//
LOCUS       DAOTAC010000323         2558 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_31342_length_2558_cov_3.935677, whole genome shotgun sequence.
ACCESSION   DAOTAC010000323 DAOTAC010000000
VERSION     DAOTAC010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31342_length_2558_cov_3.935677"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="PLM74_11010"
     CDS             <1..234
                     /locus_tag="PLM74_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016360244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMMECR1 domain-containing protein"
                     /protein_id="HON43637.1"
                     /translation="VFSVDVLSEPEPVSGPDELDPKRYGVIVGRGARVGLLLPDLDGI
                     DTTEEQIRIARRKAGIGEREPVELARFEVTRYE"
     gene            254..1249
                     /gene="amrS"
                     /locus_tag="PLM74_11015"
     CDS             254..1249
                     /gene="amrS"
                     /locus_tag="PLM74_11015"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HON43638.1"
                     /translation="MYARAEEGRGICELCPRGCKLREGAVGACRSRAMSGGRIVPRTY
                     GAVTSMSIDPIEKKPLYHFMPGAGILSVGTYGCNLSCKFCQNWEISQREADHRQLTPE
                     ELARLADSVRGQSVGVAYTYSEPLVWYEFVYDSARLVRERGLVNVLVTNGYINPEPFR
                     ELLPYVDAVNIDLKAFSPEFYESVCGGDLEPVLRSIRQAYESGCHVELTTLVVPGLND
                     SSEEMDSLARWIAELSPDVPLHVSRYFPSYRMTAPPTPISTLQVCVDAARARLHYVYV
                     GNATVPGGSDTVCPSCGKTVIRRHGYDRVEVLLSGATCPTCGAGIPVRLHGREPA"
     gene            1275..2243
                     /gene="argF"
                     /locus_tag="PLM74_11020"
     CDS             1275..2243
                     /gene="argF"
                     /locus_tag="PLM74_11020"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HON43639.1"
                     /translation="MTKALELIYDDLGRSFSGKSFLSIHDFTTEQVMRVMDLAAVLKA
                     EQKAGVPHPILKGKTLGMIFLKPSTRTRISFEVAIWQLGGYGLFLNANDLQLKRGETV
                     ADTARVLSRYLDGIMIRTFAHSDVIELAREGSIPIINGLTDLLHPCQVMADLLTGIEK
                     FGDLAGRKLAYIGDGNNMVHSLMYGGAKVGMHVACACPAGYEPDPKVVASARADGAAT
                     GAKMEVVRDPKEAVRGADILYTDVWASMGQEAEREKRVRDFQGYQINAELVNMANPDC
                     IVMHCLPAHRGEEITDDAADGPHSVLWDEAENRLHVQKAILALTMS"
BASE COUNT          515 a          705 c          839 g          499 t
ORIGIN      
        1 gtgttctcgg tggatgtgtt gtcggagcca gaaccggtat cggggccgga cgagctcgat
       61 cccaagaggt acggggttat agtcgggcgc ggcgcccggg tgggcctgct tctgcccgat
      121 cttgacggga ttgacaccac ggaggagcag atccggatcg cacggcgcaa agcggggatc
      181 ggcgagcgcg aacccgtgga actcgcgagg tttgaggtga ccaggtacga gtaaagaggt
      241 tgtcagggag gctatgtatg ccagggcaga agaggggcgg ggcatatgtg agctgtgccc
      301 cagagggtgc aagcttcgcg agggggcggt gggggcatgc cgcagccggg ccatgtcggg
      361 cggcaggatt gttccgcgca catatggagc ggtaacctcc atgtccatcg accctatcga
      421 gaagaagccc ctgtaccact ttatgcctgg ggcgggcata ctctcggtcg gaacttacgg
      481 gtgcaacctg tcgtgcaagt tctgccagaa ctgggagata tcgcagcgtg aagctgacca
      541 tcgtcagttg accccggagg aactggcacg cctcgctgat agcgtcaggg gtcagtcagt
      601 gggcgtggcc tacacatact cggagccgct cgtatggtac gagttcgtgt acgactccgc
      661 tcggctggtt cgcgagcgag ggctggtcaa tgtgctggtc accaatggct acatcaaccc
      721 agagcccttt cgggagctgt tgccttacgt tgacgccgtg aatatagacc tcaaggcgtt
      781 cagccccgag ttctatgagt cggtgtgcgg cggtgacctg gaaccggtgc ttcgctccat
      841 taggcaggcg tatgagtcag gatgccatgt cgagctgact accctggtcg tgccgggctt
      901 gaacgattcg agtgaggaga tggatagcct ggcgaggtgg atagccgagc tgtcgcccga
      961 cgtaccgcta cacgtatcgc ggtatttccc cagttaccgc atgacagcgc cgcctacgcc
     1021 gatatcgact ttgcaggtgt gcgtggatgc tgcacgcgcc aggctccact atgtttatgt
     1081 tggtaacgca actgtaccgg gaggttccga tacagtatgc ccgtcatgtg gcaagacggt
     1141 cataaggagg cacggctatg accgcgtgga ggtgctgctt tccggggcta catgccctac
     1201 gtgcggcgcc ggcattccgg tgaggctcca cgggcgcgag ccggcctaac acaaaaactg
     1261 aaggcggtgt ttggatgacc aaagcgctgg aactcatcta cgacgatctg gggcgcagct
     1321 tctcagggaa gagtttcctc tcgattcacg acttcactac agaacaggtc atgcgtgtta
     1381 tggatcttgc agcagtgctc aaggctgagc agaaggccgg agtgcctcac ccgattctga
     1441 agggcaagac tctgggcatg atcttcctga agccgtccac aaggacgagg atatcgtttg
     1501 aagtcgcaat ctggcagctg ggtgggtacg gcctgttctt gaacgccaat gatctacagc
     1561 tgaagagggg cgagactgtc gccgacactg cgcgcgttct ctcacgctac ctcgatggca
     1621 taatgatcag aaccttcgcc cactccgatg tgatcgagct agcccgcgag ggctcgatac
     1681 cgatcatcaa cggcctcacc gacctgcttc acccatgtca ggtcatggcc gacctcttga
     1741 cggggatcga gaagttcggc gaccttgcgg gccggaagct tgcctatatc ggcgatggaa
     1801 acaacatggt tcactccctg atgtatggcg gagccaaagt cggcatgcac gttgcgtgcg
     1861 cctgcccggc gggctacgag ccggatccga aggtagtagc ctcggcaaga gccgacggag
     1921 ccgcaaccgg cgccaagatg gaggtcgttc gcgaccctaa ggaggccgtg cgaggggccg
     1981 acatcctcta cacagacgtg tgggccagca tgggccagga agccgagcgt gagaagcgcg
     2041 tccgcgactt tcagggctac cagatcaatg cggagctagt gaacatggcc aaccctgact
     2101 gcattgtgat gcattgtctg ccggcgcacc gcggcgaaga gatcaccgac gatgcggccg
     2161 acggccctca ctcggtgttg tgggacgagg ccgaaaacag gctgcacgtg cagaaggcga
     2221 tactggcgct gaccatgagc taaccatgag ctaaccatga gccaaaggag agtcccgttt
     2281 ttcgcgggac ctatctgggc cgacccgggt tgcacctaga agctgtcccg tagggcatag
     2341 cgccgccgcc ggcggggagt ccctgatgcg actcccgggc ggcggcgtgt caatcatcaa
     2401 atgcgactct ggaataggcg tttggagcga ctaggcaacc aatgcaggag tgagcagtat
     2461 tcctgggcgt tacgttctcc tgtgtgggcc ctaaccgggt tctggggcat cctattcgcc
     2521 ctagagtggg attacctggt ccccgataca ggcgcatt
//
LOCUS       DAOTAC010000324         2537 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_31674_length_2537_cov_4.620064, whole genome shotgun sequence.
ACCESSION   DAOTAC010000324 DAOTAC010000000
VERSION     DAOTAC010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31674_length_2537_cov_4.620064"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="PLM74_11025"
     CDS             complement(<1..659)
                     /locus_tag="PLM74_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HON43640.1"
                     /translation="MWENVTSEEWQDWRWQLKNRITDLEQLRGAIALTSEEEEGVARC
                     LARLRMAITPYYASLIDPDDPNCPVRRQAVPVVQELHLTHADMDDPLHEDVDSPVPHV
                     THRYPDRVLFLVTSVCSMYCRHCTRRRFAGHTDRAISRDEIMEGVRYVQNTPEVRDVL
                     ISGGDPLTLTDDALEFIIRELRAIPHVEIIRIGTRTPVVMPQRITGELCTMLRKYHPI
                     WL"
     gene            complement(677..1717)
                     /locus_tag="PLM74_11030"
     CDS             complement(677..1717)
                     /locus_tag="PLM74_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-erythro-3,5-diaminohexanoate dehydrogenase"
                     /protein_id="HON43641.1"
                     /translation="MPMGCKYGTHRVIEPAGVMPQAAWKIDNTMEIRDNEILIRVQTL
                     NIDAASFTQIKKEAEYDDARIGEIMLGIVAKRGKHHNPVTGSGGMLIGTVEQVGRELA
                     GKIDLAPGDRIATLVSLSLTPLVIEKILKVHQDTDQVDIEGRAILFESGIYAKLPSDM
                     PDSLSLAVLDVAGAPAQTAKLVKPGDTVLVIGAGGKSGLLCLYEARKRAGVTGKVIAL
                     AHSAASRARAESLEYADVVLAGDATRPLEILRMIEKATSGQLADVTINCVNIPGTEMS
                     SILSTKEGGLVYFFSMATSFTAAALGAEGVGHDVTMLIGNGYTRGHAQIALEVLRESA
                     KLRKLFEELYAR"
     gene            complement(1861..>2537)
                     /locus_tag="PLM74_11035"
     CDS             complement(1861..>2537)
                     /locus_tag="PLM74_11035"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HON43642.1"
                     /translation="LDEVDAMPLPLQAKLLRVLQEGTLRRIGESAERHIDVRVISAIS
                     CSPSRAVERGLLRRDLFHRLGVIIIEIPPLRERTEDIPALTHHFVRKYSAQFGVGVRG
                     VSREVMGVFMEHAWPGNVRELQHAVEAAVQMSQGPLIGIEHLPPHIGHSGGQAWSHPA
                     AQGAGPARAEIGLIPTLQQMEENMLRYALDSCGGNVSAAARQLGIPRQTLQHKLKRYL
                     ITPSTP"
BASE COUNT          495 a          774 c          723 g          545 t
ORIGIN      
        1 agccagatcg ggtgatactt cctgagcatg gtacagagct ccccagtgat gcgctgcggc
       61 atgaccactg gcgttctcgt tccgattctg atgatctcaa catgaggtat ggcgcggagt
      121 tcgcggataa tgaactccaa ggcatcgtcc gtcaaggtca atgggtctcc gcccgatatg
      181 agaacatccc gcacttcggg cgtgttctgt acgtagcgga cgccttccat aatctcgtcc
      241 cgcgatatcg ctctgtcggt atgacccgca aacctccgcc tggtgcaatg acggcagtac
      301 atcgagcaga ccgaggtcac cagaaacaga acgcggtcag gatatctgtg tgtcacgtga
      361 ggtaccggag aatccacatc ctcgtgaagc ggatcgtcca tgtcggcatg ggtcaggtga
      421 agttcttgaa ccaccggaac ggcctgtctt ctcacggggc agttgggatc gtcaggatcg
      481 atgaggcttg catagtaagg cgttatcgcc atacgcagcc tcgcaagaca cctggcgact
      541 ccttcttcct cttcggacgt gagcgcaata gcgcctcgca gctgctcaag gtcggtgatt
      601 ctgttcttaa gctgccatcg ccagtcctgc cactcctcgc ttgtgacatt ctcccacatg
      661 ctcaagacct ccgcatctat ctggcataaa gctcctcgaa gagcttacgc agctttgcgc
      721 tttcgcgcag aacctccagc gcaatctgcg cgtgcccgcg cgtgtatccg tttccaatga
      781 gcatcgtcac atcatggcca actccctcgg cacccagggc ggccgcggta aagctcgtcg
      841 ccatgctgaa gaagtagacc agcccgcctt ctttggtgga tagtatggat gacatctcgg
      901 ttcccggaat gttcacacag ttaatcgtaa cgtctgccag ttgcccactg gtagctttct
      961 cgatcatgcg caagatctcc agcggacgcg tggcgtctcc ggcgagcacc acgtctgcgt
     1021 attccaacga ttcggccctg gcgcgactgg cagctgagtg agccaaggct atcaccttcc
     1081 cggtgacccc ggcgcgcttt cgcgcctcat acaagcagag cagcccggat ttgccgccag
     1141 cgccgataac gagcaccgtg tcgcctggct tgactaactt ggcggtctga gcgggagcgc
     1201 ccgccacgtc cagcacagcc agtgacaggc tgtcgggcat gtcgctgggc agcttggcgt
     1261 atatcccgct ctcaaacagg atagcgcgac cctcgatatc cacttgatcg gtgtcctgat
     1321 gaaccttgag gatcttctcg atcacaagag gcgtgagcga cagcgacact aaggtggcga
     1381 tcctatcgcc gggcgcgaga tcgatcttgc ccgctagctc gcggccgacc tgctcaaccg
     1441 tgccgataag catgccgccg gacccggtaa ccgggttatg gtgcttgccg cgcttagcga
     1501 cgatgcccag catgatctcg ccgatccggg cgtcgtcata ctcggcctcc ttcttgatct
     1561 gggtgaaact tgcggcgtcg atgttcaggg tctgcacacg gatcaggatt tcattgtcgc
     1621 ggatctccat cgtgttatcg atcttccacg cggcctgcgg catgacgccg gcgggttcga
     1681 ttactcggtg ggttccgtac ttacacccca tgggcatttc acgaacctcc catcatggct
     1741 ttcagactac aggtgatgca agactcgcgc caacgggaat ccctgccctg agtgggctct
     1801 agagcatctg gggcgtgccg caaattgggc aactgcccat ccacgggcac cttcgaggct
     1861 tcatggggtg gaaggcgtga tgaggtagcg cttcagctta tgttggagtg tttggcgtgg
     1921 gatgcccagc tgacgcgcag cggcggagac attgcctcca catgaatcca gcgcgtaacg
     1981 gagcatgttc tcttccatct gctgcaatgt gggaatgagc ccgatttcgg cacgggcagg
     2041 gccggcgccc tgcgcagccg gatggctcca ggcctgtcca cctgagtgcc cgatatgagg
     2101 cggcaagtgc tcaatgccga tgagcggccc ttgcgacatc tggactgcgg cctccacagc
     2161 atgctgcaat tcgcggacat tgcccggcca ggcgtgctcc atgaagactc ccatgacctc
     2221 tctagagacg cctcgaacgc ccacaccgaa ctgcgcgctg tactttctca caaaatgatg
     2281 agtaagtgcc gggatgtcct cggtgcgctc ccgaagcggg ggtatttcta tgatgatcac
     2341 cccgagccta tggaaaaggt cacggcggag caagcctcgc tctacagccc gagatgggct
     2401 acaggatatg gctgaaatca ctcttacgtc gatgtgccgt tcggcggatt cgcctatccg
     2461 cctaagagtg ccttcctgca gcaccctaag cagcttggcc tggagcggca gcggcatggc
     2521 atctacttcg tcaagaa
//
LOCUS       DAOTAC010000325         2536 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_31685_length_2536_cov_7.724708, whole genome shotgun sequence.
ACCESSION   DAOTAC010000325 DAOTAC010000000
VERSION     DAOTAC010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31685_length_2536_cov_7.724708"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2327
                     /locus_tag="PLM74_11040"
     CDS             <1..2327
                     /locus_tag="PLM74_11040"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HON43643.1"
                     /translation="SKRLVVPATGHNVPAEAIHPLLFDFQRDIVRWALRKGRAAIFAE
                     CGMGKTLMQIEWARHVAEHTGGRVLILAPLAVAHQTVGEGAKIGVQVAYMRGQDQIEN
                     ERLVITNYEMAARFDPSEFAGVVLDESSILKAFTGKTKRMLINMFGQTPYRLCCTATP
                     APNDHLELGNHSQFLGVMDSNAMISRWFINDTMAAGSYRLKRHAERDFWRWVTSWAVC
                     ISKPSDLGDAYSDHGFMLPELRIHERVVRVDHTRAFEQGQLILAEAPSATSMWREKRA
                     TMADRCRTACDIVGDSDEPWIIWCDTNAEADELKRLFPKATEVRGSDSIREKERKLIE
                     FSEGRARQIITKPDIAGFGLNWQHCSRQAFVGVTYSFEKTYQALRRSWRFGQTRPVDA
                     YLIYAETEGNVRQTLAEKREAHRQMQSAMNEAMRENGLEGITNERGHRLNVRRDEAVG
                     EGWRLLLGDCIERVKELETASVDFTIFSPPFSTLYIYSDLPEDMGNCEDDEEFFRHFG
                     YLIPELLRVSKAGRLCAVHCKDLPLYRNRDGVAGLRDFPGEIIRAFESAGWTYHSRVT
                     IWKDPVIEMQRTKNHGLLWKNFTSRGEVCRQGMPDYLVVFRKWADEMPDKQVRHEPSS
                     YIGIEPPTSWDGQRDFSIQVWQKYASPVWFDINQMRVLNVEQARDSQDEKHICPLQLD
                     VIGRCIEIWTNPGDLVFSPFAGIGSEGYEAVRMKRRFLGIELKESYWQAAIGHLERAA
                     LEANERTLFEFAERLAKGEVFGDDIRRDHGPIGA"
     gene            2290..>2536
                     /locus_tag="PLM74_11045"
     CDS             2290..>2536
                     /locus_tag="PLM74_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43644.1"
                     /translation="MTYDEIMALSGRELDAAVAERVMGHKALAHAQTPFYSRDIADAW
                     LVHERMIADWDTLWDYAREMNALIGSRGIRKIDVTMGL"
BASE COUNT          577 a          655 c          835 g          469 t
ORIGIN      
        1 aatcgaagcg gttggtagtg cctgcgacag ggcataacgt gcctgcggag gcgatacatc
       61 ccctgctgtt tgacttccag cgggacatcg ttaggtgggc tttacgcaag ggccgagcgg
      121 cgatatttgc ggagtgcggc atgggcaaaa ctctgatgca gatcgagtgg gcacgacatg
      181 tcgcggagca caccggaggc agggtactga tcctcgcgcc gctcgctgtg gctcaccaga
      241 ccgtgggtga aggcgctaaa atcggcgtcc aggtggcata tatgcgcggc caagatcaga
      301 tcgaaaacga gcgactggtg attaccaact acgaaatggc cgcgcgcttt gatcccagcg
      361 agttcgccgg agtggttctt gacgagagct cgattctgaa ggcgttcacg ggcaagacga
      421 agcgcatgct cataaacatg ttcgggcaga cgccgtatcg cttgtgctgc acggcgactc
      481 ctgcgcccaa tgaccacttg gagctcggca atcattctca gttcctcgga gtgatggatt
      541 cgaacgcgat gatctcccga tggttcatca acgataccat ggcggccggt agctaccggc
      601 tgaaacgcca cgcggaacgc gacttctggc gctgggtgac gagctgggcg gtatgcatct
      661 ccaaaccctc ggacctgggg gatgcgtatt ccgatcatgg gttcatgctt cccgaactac
      721 ggatacacga gcgcgtcgtg cgcgtagacc atacccgtgc cttcgaacag gggcagctga
      781 tactcgcgga agctccgtcg gccacgtcca tgtggcgaga gaagcgagcc acgatggccg
      841 acaggtgccg gactgcgtgc gacatcgtgg gcgactccga cgagccatgg attatctggt
      901 gcgacacgaa cgcggaggct gacgagctca agagattatt cccaaaggca acggaggttc
      961 ggggttcgga ttcgatacgc gaaaaggaac gcaaactcat tgagttctcc gaagggcgtg
     1021 cccgacagat aatcaccaaa ccggatatcg ccggcttcgg actcaactgg cagcactgct
     1081 ctcggcaggc gttcgtgggt gtgacctatt cgttcgaaaa gacgtatcag gcactccgca
     1141 ggtcatggag attcggacag acccggccag tggacgcata tctgatatac gccgaaaccg
     1201 agggcaatgt acggcagacg cttgcagaga aacgggaggc gcatagacaa atgcagagtg
     1261 cgatgaacga ggcgatgagg gagaacggac tggagggaat aaccaatgag aggggtcatc
     1321 gtctgaacgt tcgacgggac gaggccgtgg gcgaaggctg gcggctgctg ctgggcgact
     1381 gcatagagcg ggtcaaggag ttggagacgg cgagcgtaga tttcacaatc ttcagcccgc
     1441 cgttctccac gctctacata tactccgacc tgcccgagga tatgggcaac tgcgaggacg
     1501 acgaggagtt ctttcggcat ttcggctacc tgatcccaga gcttctgagg gtatcgaagg
     1561 ccggcaggct ctgcgctgtt cattgcaaag acctgccgct atacaggaac cgggacgggg
     1621 tcgcgggttt gcgagacttc ccgggggaga tcatccgcgc atttgagtcc gcggggtgga
     1681 cctatcattc cagagtcacc atctggaagg acccggtgat cgagatgcaa aggaccaaga
     1741 atcacggcct gctgtggaag aatttcactt cacgcgggga agtgtgccgg cagggcatgc
     1801 ccgactactt ggtggtgttt cgcaagtggg ctgacgagat gcccgacaaa caggttcggc
     1861 acgagccaag tagctatatc gggatagagc cgccgacaag ctgggacggt cagcgcgact
     1921 tctcgattca ggtatggcag aaatacgcga gccccgtatg gttcgatatc aatcaaatgc
     1981 gggtgcttaa tgtggaacag gcgcgggaca gccaagacga aaaacatatt tgcccattgc
     2041 agctcgacgt aataggccgg tgtatcgaaa tctggacgaa cccgggagat ttggtgttca
     2101 gcccgttcgc ggggatcggg tcggaaggct atgaggcggt gaggatgaag cgccggtttc
     2161 tcgggatcga gctgaaggag agttactggc aggcagcaat cggccatctg gagagagcgg
     2221 cgctggaggc taacgaacgg acgttgttcg aattcgcaga aagactcgca aaaggggagg
     2281 tattcggcga tgacatacga cgagatcatg gccctatcgg ggcgtgagct ggacgcggcg
     2341 gttgccgagc gggtcatggg gcacaaggcg ttagcgcacg cacagacacc cttctactcg
     2401 cgcgatatcg ccgacgcctg gctggtgcac gagcgcatga tcgcggactg ggacacgctc
     2461 tgggactatg cgcgcgagat gaacgcgctg atcggcagcc gaggcatacg caagatcgat
     2521 gtcacgatgg ggctat
//
LOCUS       DAOTAC010000326         2533 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_31733_length_2533_cov_4.630751, whole genome shotgun sequence.
ACCESSION   DAOTAC010000326 DAOTAC010000000
VERSION     DAOTAC010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31733_length_2533_cov_4.630751"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..105
                     /locus_tag="PLM74_11050"
     CDS             <1..105
                     /locus_tag="PLM74_11050"
                     /inference="COORDINATES: protein motif:HMM:NF016114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HON43645.1"
                     /translation="VNKASAILGALRTGVVDVLVTDDVAARKVLEMER"
     gene            144..761
                     /locus_tag="PLM74_11055"
     CDS             144..761
                     /locus_tag="PLM74_11055"
                     /inference="COORDINATES: protein motif:HMM:NF018816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HON43646.1"
                     /translation="MPLGQGALGLGVSYALALGYLVLMWFLGSILSRQYPVEHRVAWD
                     TRTIARVTILAAVAGAGAMIKIPGPATTIALDAAAGYFAASFFGWPVGATVAAIGCFL
                     ANALGGFASWLPMVPIYLAGMALTVTIAVFVAKKVNIVVAIIVGTIVNTITCLGPWAL
                     MIGTPLMIALLPSQIIGSAVNALVGLSVAEALKAAQNRKRPESID"
     gene            779..2530
                     /locus_tag="PLM74_11060"
     CDS             779..2530
                     /locus_tag="PLM74_11060"
                     /inference="COORDINATES: protein motif:HMM:NF010167.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HON43647.1"
                     /translation="MASDIISLKDVTFSYKGSKRRALDGITLNVKQGECVAILGPTGA
                     GKSTLACVMNGTAPRFMDGSLAGDVTVDGMVPAKVGTAQMASRVGLVFGDPDTQLFGM
                     TVEEDVAFGPANLGLGYESIMERVGKSIADMRLSGLERRAPHRLSGGQKQATAIAGVY
                     AMLPKIMVLDEPTSMLDPEGKARVFGIVRMLKEQLGVTVVLIEQEVDDILNLADRVFV
                     MRRGAFVLSGAPREVFGRVEELRQAGVRVPQIVEFGTLIGAAETPFTIEEAISAITPS
                     SAGVGEVAAPACDRTKPDLAAAPEAGRAAQADPVVQAVDVEFTYPGGARALDGVNLAV
                     GRGDFVAIVGQNGAGKTTLTRHFNGLLRPSSGDIRVLGRSAQGVPTIELCRHVGYVFQ
                     NPDEQLFCTSVQEELKFGPTNIGADPHVTSQRVEEILGDIGLTKYREVWPKYLTKGER
                     QRLALASVVAMDPDVLIVDEPTTGLDWVESLQILDYLERLRDEHKKTIIIVTHDMNIV
                     SLYARRVVVMARGRVVGDGTPREIFSQPDLMELACLRRPPIADLSQALWGEVLLTPAE
                     AAARLLAVKSQATGVCS"
BASE COUNT          501 a          690 c          838 g          504 t
ORIGIN      
        1 gtcaacaagg catcggcgat actaggcgct ctgcgtactg gggtggtcga cgtgcttgtc
       61 accgacgatg tggccgcccg caaggtgctc gagatggaac ggtaagccag gggcttgatc
      121 aatctactaa ggaggcagga caaatgccac ttggacaggg ggcattgggg ctcggtgtgt
      181 cgtacgcgct cgctctaggt tatctggttc tcatgtggtt tctcggaagc atcctttcaa
      241 ggcagtaccc tgtcgaacac cgcgtggcgt gggacacgcg cacgatagcc agggtcacga
      301 ttctggcagc tgtggcgggc gcgggggcca tgatcaagat accggggccc gctactacca
      361 ttgcgcttga cgctgccgca gggtacttcg ctgcttcatt cttcggttgg cccgtcgggg
      421 ctacagtagc cgcaatcggc tgttttctgg ccaacgccct cggggggttt gccagctggt
      481 tgccgatggt gccgatctac cttgcgggga tggccctcac agtcactata gccgtgtttg
      541 tggccaagaa agtcaacatt gtcgtcgcca tcatcgtggg cactatcgtc aataccatca
      601 catgtctggg cccatgggcg ctgatgatcg gcactccgct gatgatagcg ctcctgccgt
      661 cgcagataat agggtctgcc gtcaacgcgc tggtggggct gtcggtggcg gaagcgctca
      721 aagctgcgca gaacaggaaa agaccggaat ccattgactg acgcaagggg tgttctactt
      781 ggcgtccgac atcatctcgc tgaaggatgt aaccttcagc tataaaggat caaaacgccg
      841 cgcgctcgac gggatcacac tcaacgtgaa gcagggtgaa tgcgtggcca tactcgggcc
      901 gactggcgcc gggaaaagca cgctggcgtg tgtcatgaac ggaacggctc cgcgtttcat
      961 ggatggcagc cttgctgggg acgtgactgt agacggaatg gttcctgcaa aggttggcac
     1021 agcccagatg gcgtcccggg ttggtttggt attcggcgat cccgacacgc agctgttcgg
     1081 aatgactgtt gaggaagacg tggcctttgg ccccgcaaac ctggggttgg gctatgaaag
     1141 catcatggag agagtgggca agtccatagc tgacatgcgt ctttcagggc ttgagcggcg
     1201 ggctccgcac aggctctcgg gcgggcagaa acaggccacg gccatagccg gcgtctatgc
     1261 catgttgccc aagataatgg tgctggatga gcccacgtca atgctcgacc cggagggaaa
     1321 ggcccgagtg ttcgggatcg tgaggatgct caaagagcag ctcggagtga ctgtggtcct
     1381 gatcgagcag gaagttgacg acatactgaa cctcgccgac agggtgttcg tgatgcgccg
     1441 aggcgcgttc gtactgtccg gggctccacg cgaagtcttt ggaagagtag aggaactgag
     1501 gcaggcaggt gtacgcgttc cgcagatcgt ggagttcggc acgctcatcg gggccgctga
     1561 gactccgttt acgatcgagg aagccatctc ggctattacg ccatcttctg ccggcgtggg
     1621 cgaagtagca gctcccgcat gcgataggac gaagccggat ttggctgcgg cgcccgaggc
     1681 aggccgggcg gcgcaggccg accccgtcgt acaggccgtc gacgttgagt tcacctatcc
     1741 tggcggcgcg cgggcgctgg acggagtgaa ccttgccgta ggccgcggcg acttcgtggc
     1801 catagtcggc caaaacggag cgggcaagac caccttaact cgccacttca atgggttact
     1861 ccggccgtcg tccggcgata tcagggtact aggccgatca gcgcagggcg tgcccactat
     1921 agagttgtgt cggcatgtgg ggtatgtctt ccagaaccct gatgagcagc tcttctgcac
     1981 cagcgtgcag gaggagctga agttcgggcc cacaaacatc ggggcggacc cgcatgtgac
     2041 gtcacagcgc gtcgaggaga tactgggcga catcggtttg accaagtatc gcgaagtatg
     2101 gccgaagtac cttaccaagg gagagcggca acggcttgcg ctggcgtcag ttgtggccat
     2161 ggatccggac gtgctcatag tagacgaacc caccaccggg ctcgattggg tggaatcgct
     2221 tcagatcctc gattatctgg aacgactgcg cgatgagcac aaaaagacga tcatcatagt
     2281 cacccacgac atgaacatcg tgagcctgta cgctcggcga gtggtggtca tggccagggg
     2341 tcgggtagtg ggggacggaa ccccgcgcga gatattctcg caacccgatc tgatggagct
     2401 tgcatgcctc agacggcctc ccatcgccga tctgtcgcag gcgctgtggg gcgaggtact
     2461 cttgaccccg gctgaggccg cggctaggct tctcgccgtc aaatctcaag ccacgggggt
     2521 gtgttcgtag tga
//
LOCUS       DAOTAC010000327         2501 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_32231_length_2501_cov_4.467702, whole genome shotgun sequence.
ACCESSION   DAOTAC010000327 DAOTAC010000000
VERSION     DAOTAC010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32231_length_2501_cov_4.467702"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..254
                     /locus_tag="PLM74_11065"
     CDS             102..254
                     /locus_tag="PLM74_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43648.1"
                     /translation="MATVDEAGIPRLQSWEAQHLKHILANLTAYRDALVANNPDPGKS
                     GGWGDY"
     gene            complement(262..1362)
                     /locus_tag="PLM74_11070"
     CDS             complement(262..1362)
                     /locus_tag="PLM74_11070"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HON43649.1"
                     /translation="MSHTLRVPLAKPKPDFDELLQVLRGEKRARRVHFAELFVDGEIV
                     SKVLSDFLGERPIPSPEHDFEGYWDQQIKFWLYMGYDYIRVSGGLSIPEVRHRVTGDT
                     AALSRGERGWVEEGIGVIKSWADYDAYPWHVMEKMDFTAYEYVSQHLPDGMKMLVCPS
                     SGVFEISSEYLLGFEGMSMMLYEDPALVEAVFNRVGQCILDFYRNVTTLSNVAGIFQG
                     DDLGFKTSTFLSPTHLRQLVLPWHKRYAALAHEQGQVYWFHSCGNLAGVMDDIIDDVR
                     IDAYNSFQDEIIPVADFVDTFGHRVAALGGVDVDCLCRMEEDDLRKYVRHITDHCAQK
                     RWALGSGNSVPNYAPVRNYLVMLDEGLRWQAP"
     gene            complement(1481..2278)
                     /locus_tag="PLM74_11075"
     CDS             complement(1481..2278)
                     /locus_tag="PLM74_11075"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HON43650.1"
                     /translation="MACRIIDSPRERLVAILTNNFAPCNGRFPTLIALASLIAAGSIK
                     ASLLVAAMVLLGMAASLGMSWVLSRTVLRGVPSSFILELPPYRMPQVARVLWRSLYDR
                     TLLVLRRAVIVAAPAGAIVWAIANMTTPAGESILTSAARVLHPAAAYMGLDGFILTSF
                     ILGLPANEIVLPILVIAYSSSTALTETTGLAPLGRILAANGWTWLTAVNTMIFSVLHF
                     PCSTTLLTIGAETKSPRWTALAALMPTALAIAVCCATRAVARLLGFA"
BASE COUNT          536 a          733 c          745 g          487 t
ORIGIN      
        1 atcctagaag aaggactgcg tattgtaaac acggtttagc attagtttgt acggcgggag
       61 ctgccggaag ttacgcctgt ggaaaccgtg caagaccagc aatggcaacg gtcgatgaag
      121 caggaatccc gcgacttcag tcgtgggagg ctcaacatct caagcacata ctggcaaacc
      181 ttaccgctta ccgcgacgcg ctagtcgcga acaaccccga tcccgggaaa tcgggcgggt
      241 ggggcgacta ctagttcgcg ctcatggagc ctgccagcgc aggccctcat ccagcatgac
      301 aaggtagttt cgcaccggag cgtagttggg cactgagttt cctgagccca gtgcccatcg
      361 tttttgggcg caatgatctg tgatgtggcg gacgtacttc cgaaggtcgt cttcctccat
      421 cctgcacagg caatcgacgt ccacgccgcc cagcgcggcg actcggtggc cgaacgtgtc
      481 gacgaagtca gccacgggga ttatctcgtc ctggaatgag ttgtaggcat cgatcctgac
      541 atcgtcgatg atgtcatcca tgacaccggc gagattaccg caggagtgga accagtacac
      601 ctggccttgc tcgtgggcga gagccgcgta tcgtttgtgc catggaagaa ccagctggcg
      661 caggtgcgta ggcgacagga aggtggaggt cttaaagccc aggtcatcgc cttggaatat
      721 gcccgccacg ttgctgaggg tggttacgtt acggtagaaa tccaggatgc actggcccac
      781 acgattgaaa actgcctcaa ccagcgccgg atcttcgtac agcatcatgc tcatgccctc
      841 aaagcccagc aggtactcgc tggagatctc gaacacaccg cttgatgggc agacaagcat
      901 cttcattcca tctgggaggt gttgggagac atactcgtac gccgtgaagt ccatcttctc
      961 catgacgtgc cacgggtagg catcgtagtc cgcccacgac ttaatgaccc caatcccttc
     1021 ctcgacccaa ccgcgttcgc ctcgcgacag agcggcggta tcgccggtca cgcggtgcct
     1081 aacctccggg atggacagcc cgcccgacac tctgatgtag tcgtatccca tgtagagcca
     1141 gaacttgatt tgctggtccc agtagccttc gaagtcgtgt tcgggggagg ggataggccg
     1201 ctcgccaagg aaatcggaaa gcaccttact gactatttcg ccatcaacga agagctctgc
     1261 gaagtgcacg cgacgggccc gcttttcgcc ccggagcacc tgtagcaact catcaaagtc
     1321 gggtttgggt ttggccagcg gaactctcag agtatgcgac atccgattgc ccacctttcc
     1381 cggatcgcaa gagcgtagta gttataccac tgacgcctac atgctacatg acgtgtgtcc
     1441 agccgtcaag cagctcggtt gcggggcccc ggtgtcggga tcacgcgaac ccgagtagcc
     1501 tggccactgc ccgagtggcg cagcaaaccg cgatcgccag agccgtaggc atgagcgcgg
     1561 ccaacgctgt ccagcgaggg ctcttggtct cggcgcctat ggtgagcagt gtggtgctgc
     1621 acggaaagtg tagcactgag aagatcatgg tattcacggc ggtcagccat gtccagccgt
     1681 tggctgccag gatccggcct aggggcgcca gaccggttgt ttcggtcagc gctgtggagg
     1741 acgagtacgc tataaccaga ataggcagga cgatctcgtt cgccggcaaa cccagaatga
     1801 acgaggtcag gatgaacccg tccagcccca tataggcggc ggcaggatga agaacgcggg
     1861 ctgcggatgt caggatgctc tcgccggcag gagtcgtcat gttggctata gcccacacga
     1921 tagctcccgc cggcgcagct acgattactg cccttcggag caccaggagc gtgcggtcgt
     1981 acagtgagcg ccacagcact cgggccactt ggggcattcg gtacggcggt aactccagga
     2041 tgaaggaaga cggcacgccc cgaagtacgg ttcgggagag cacccacgac atgcccaacg
     2101 aggccgccat tcccagaagc accatggcgg ccacaagaag cgacgcctta atcgatcctg
     2161 ccgcgatcaa agaggccaac gcgatcaggg tcgggaatct tccgttgcac ggcgcgaagt
     2221 tgttggtcaa gatcgccact aggcgttcgc gtggcgagtc gattattctg caggccatga
     2281 cgccggctgc attgcaccca aatcccatgg ccatggatcg gcagttttcg gtatgcctct
     2341 cgctcctaga tgagacactc cttgatgcta tcctgcaccg agaagcggac atggattctg
     2401 atggtctggc gcttgacgcg gccctgcgct gttcttacgt agctgccttg cccgatctcg
     2461 atgcggtcta tcaaccggaa gagcaaatcc tgcgatagcc c
//
LOCUS       DAOTAC010000328         2414 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_33665_length_2414_cov_4.127173, whole genome shotgun sequence.
ACCESSION   DAOTAC010000328 DAOTAC010000000
VERSION     DAOTAC010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33665_length_2414_cov_4.127173"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..431)
                     /gene="groES"
                     /locus_tag="PLM74_11080"
     CDS             complement(144..431)
                     /gene="groES"
                     /locus_tag="PLM74_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009936171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HON43651.1"
                     /translation="MMLRPCGDRVLVKPNVEEERTAGGIVLPDTAKERPQWGEVIAVG
                     PGKWDEDGKQRIPMDVKAGDKVVFAKYGGTEVKVDGEEYLILRESDILAVK"
     gene            605..1336
                     /locus_tag="PLM74_11085"
     CDS             605..1336
                     /locus_tag="PLM74_11085"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HON43652.1"
                     /translation="MPDLLRLLAVTVIPGVLWVLYFHRKDKYEREPGGLVAKTFLMGA
                     VMIIPAGIVESLFRGILRNPPSLAALLLAAVFGVGFVEEFFKYWAVRRVAYDHPAFNE
                     PVDGIIYAVSAGLGFAAFENVLYASTLGLGVGLVGAVLTSLVHASFSGIVGFGMGIAK
                     FAPRGIQGAVIGRSLMLAALLHGVYDFLLMSQLMNFYLTVAVVILLHSLLLGRIRFAL
                     KLSPFGSPGEAGVEINREDIDDDDE"
     gene            1440..2204
                     /locus_tag="PLM74_11090"
     CDS             1440..2204
                     /locus_tag="PLM74_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008518956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HON43653.1"
                     /translation="MSGHSKWANIKHKKAKADAQRGAAFTKAARELIVAVKQGGPDPE
                     GNFRLRMAIQAAKAVNMPNDTIQRAIKKAAGDADGENYEEVVYEGYGPAGVALIVEAM
                     TDNRNRTASDVRYLFSRHGGSLGETGCVSWMFDRKGTVTVDSENYPDGEDGMMLIAME
                     AGAEDLRVYDDYYEMLTSPEDLDAVRRAVEAAGVECSEARIVRVPKTVMTLGLKEAAS
                     AMRLVDALDEHDDVQHVFTNFDIPDEVLEQLESEES"
     gene            2307..>2414
                     /locus_tag="PLM74_11095"
     CDS             2307..>2414
                     /locus_tag="PLM74_11095"
                     /inference="COORDINATES: protein motif:HMM:NF014166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HON43654.1"
                     /translation="MIVLGIDPGTAITGYGLVREERGSLTCVGMGAVTTR"
BASE COUNT          475 a          643 c          752 g          544 t
ORIGIN      
        1 ccttgactgc cgacgcgact gcatctactc cgtttttcag agcggtgcgc gcatcctctt
       61 tgtatgccaa ctgctttgca gccatgagcg taatacctcc cccatatgtg gattcttcga
      121 tttgagcaaa atgctacgac ggtctacttg accgcgagaa tatcggactc gcggaggatg
      181 agatactctt ctccatccac tttgacttca gttccgccgt acttggcaaa gacgactttg
      241 tccccggcct tgacatccat ggggatccgc tgcttgccgt cttcgtccca cttgccggga
      301 cctactgcga tcacttcgcc ccactgggga cgctctttgg cggtgtcagg aaggactatg
      361 cctccagctg tcctctcctc ctcgacgtta ggcttgacaa gaaccctgtc gccacaaggc
      421 ctaagcatca tgatttaccc ccttcacggc aatcctctgt ttcgttagca ctcaaccgag
      481 gcgagtgcta acaacgttat gatacacgca ctgttttagg gttgtcaagg cttatgatag
      541 aattatttcc ggcattggcg atgctatacg atgagagcta cttcaagaaa ggtggatgct
      601 tgacatgccc gatctcctgc ggttgctcgc ggttaccgtg atcccgggcg tcctctgggt
      661 gctctatttc catcgcaagg acaaatacga gcgtgagccg ggagggcttg tcgcgaagac
      721 gttcctgatg ggtgcagtga tgattatccc ggcaggcatc gtcgagtcgc tgttccgagg
      781 catcttgcga aacccgccga gcctggccgc tttgctgctt gcagctgtgt tcggagtggg
      841 gttcgtcgag gagttcttca agtactgggc cgtgcggcgt gtggcgtatg accatcctgc
      901 attcaatgaa cctgtagacg gcataatcta cgccgtctcc gccgggctgg gtttcgccgc
      961 gtttgagaat gtgctctacg catcgactct gggcctcggg gtcgggctgg tcggagctgt
     1021 cctgacctca ctagtacatg cttccttctc aggaatcgtg gggtttggca tgggcatcgc
     1081 caagtttgcg cctcggggca tccagggggc cgtgataggg cgcagcctga tgttggcggc
     1141 cctgttgcat ggtgtgtacg acttcctcct catgtcgcag ctgatgaact tctacctgac
     1201 ggtggcagtg gtcatattgc tgcacagcct gctcctcggt cgcataaggt tcgcactgaa
     1261 gttgtcgcct ttcggcagcc cgggagaggc gggagtggag atcaatcgcg aggatatcga
     1321 cgacgacgat gaatgattga gccggtcttg tggctctgcg cgcttccaag cgcggcaatg
     1381 ctgtgctaga atgtataagg caatgctggc cgggtttccg aaagaagggg ggaggcagca
     1441 tgtccggtca ttccaaatgg gccaacataa agcataagaa ggccaaggcg gacgcacagc
     1501 gtggggctgc gttcaccaag gcggcccgcg agctcatagt cgctgttaag cagggtgggc
     1561 ctgaccccga gggtaatttc aggctgagaa tggctattca ggctgctaag gccgtgaaca
     1621 tgccgaacga cacgatccag cgtgcgataa agaaagctgc cggcgacgcc gacggcgaga
     1681 actatgaaga ggtcgtgtac gagggatacg ggccggcggg cgtggcgctg atcgtcgaag
     1741 cgatgaccga caatcgcaac cgtaccgctt cagacgttcg ctacctgttc tcgcgccacg
     1801 ggggttcctt gggcgaaaca ggctgcgtta gctggatgtt cgatcgaaaa ggcacggtga
     1861 ctgtggattc agagaactac cccgacggag aagacggcat gatgctcatt gccatggagg
     1921 ccggggcgga ggatctcaga gtatatgacg attactacga gatgcttacg tcgcctgagg
     1981 atctcgacgc ggtgcgccgc gcagtggaag cagccggcgt tgagtgttcg gaggcgcgta
     2041 tagtcagagt tcccaaaacc gtcatgactt tggggctgaa agaggccgcg tctgcaatgc
     2101 ggctcgtgga tgctttggac gaacatgacg acgtgcagca cgtcttcaca aacttcgaca
     2161 tacctgacga agtccttgag cagttggaga gcgaggagtc ctgacgtcgg gcacaatcgc
     2221 ttgcggggcg accacgctct cggcgtggcc gcttttcttt cagtggatga atgcggcgac
     2281 ctgctacggc gcgggaggtg ggagtcatga tagtgctggg gatagatccg ggcacggcca
     2341 taaccggata cggccttgtg cgcgaggaac gcggctctct tacgtgcgtg ggtatggggg
     2401 ccgtgacgac cagg
//
LOCUS       DAOTAC010000329         2405 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_33821_length_2405_cov_5.586383, whole genome shotgun sequence.
ACCESSION   DAOTAC010000329 DAOTAC010000000
VERSION     DAOTAC010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33821_length_2405_cov_5.586383"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="PLM74_11100"
     CDS             <1..512
                     /locus_tag="PLM74_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HON43655.1"
                     /translation="VTFISNYLVLRDGIFGLTRLFGGGSLINTWTGYVVSTMVGGSAV
                     FIMKQFFEGLPTELEESARIDGASVLKTYSRIMLPLAGPALGALAILTFQGTWNEFFW
                     PLVILTSPPDKYTLTLGLLSFRTTYAVAFDWGPMLAGTVMSALPIVVMFIVFQRYFVE
                     GISFSGIKG"
     gene            complement(536..>2405)
                     /locus_tag="PLM74_11105"
     CDS             complement(536..>2405)
                     /locus_tag="PLM74_11105"
                     /inference="COORDINATES: protein motif:HMM:NF015588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43656.1"
                     /translation="GLRQLDVLGVHSPKRHGASPGVAGLDACTINSHIPIAVALASAA
                     FVESADSDSDPDPAGPAQCAASRRHLEPTPLGRPYAAGHQFRAYLRAGDRLVLEKYAV
                     YRTGRDVRSACAEPEPASREASPAPLATPGPSSLLDAACQSAEAAARVGYDLLFAAHC
                     REWVARWSRCDVRIWGPDYDQLGMRFAIFHMLVCAPDTDDRVSIGAKGLHGEGYRGHV
                     FWDTEAFVHPFFCAAFPDAARNLLMYRYHTLPGARRKACERGYRGAMFAWESADSGDE
                     TTPAWTKPDPATGRRIRIWCGDTEDHITADVAHAVWSYWQWTGDDDFFRDYGAEIVIE
                     AGRFWAGRAAWNEEMRRYEYLRVIGPDEYHENIDNNAFTNYMAKWTIDAALSASEWMM
                     ANHPSTWKELSGRLGLTLREQDEMRRVAQAVYIPQPDPDTKVIEQFDGFFDLEDITGS
                     IRDLIDAGRSADMALIQDMARHRRILKQADVVMLAKLMPNLFGTDVWTANYDYYSART
                     AHGSSLSPSAHALVAARLTRVGQAYQHFSRSLALDLCDSMGNLGDGIHMANLGGIWQA
                     AVFGFAGVRLDPQAEHGICIAPALPDAWDRLETAVVVHGRRARLVVTRYRTELTWE"
BASE COUNT          427 a          748 c          746 g          484 t
ORIGIN      
        1 aggtgacgtt catctccaac tacttggtcc tccgggatgg catattcgga ctgactcggc
       61 tcttcggagg cggttcgctg atcaacacct ggaccggcta tgtggtatct accatggtgg
      121 gcggcagcgc ggtgttcata atgaagcagt tcttcgaagg actgcccacg gaactggagg
      181 aatccgcccg cattgacggg gcgtctgtcc tcaagaccta ctctcgcatc atgctgcccc
      241 tggccggccc ggccctgggg gcgctggcga tcctcacctt tcaggggacg tggaacgagt
      301 tcttctggcc tctcgtgata ctcacgtcgc ctcccgacaa gtacaccctg acgttgggcc
      361 tattgagctt caggactact tacgcagtgg cgttcgactg ggggccgatg cttgccggca
      421 ccgttatgtc ggccctgccg atagtggtca tgttcatcgt cttccaaagg tacttcgtcg
      481 agggcatcag tttctcaggg ataaagggct agcgtcgtcc gggtaactgc gtcgcctatt
      541 cccacgtcag ctcagttcga tacctggtga cgaccagcct ggcccgtctt ccatgcacca
      601 ctactgcggt ctctagcctg tcccatgcgt caggcaacgc gggcgcgatg cagatgccgt
      661 gctcagcctg cgggtcaagc ctaacccctg caaatccgaa cactgcggcc tgccatatcc
      721 cgcccaggtt ggccatatgg attccgtctc ccagattgcc catggaatcg cacaggtcga
      781 gggcgaggct acggctgaag tgctggtagg cctggccgac ccgggtcagc cgtgccgcga
      841 ccagcgcgtg agccgatggg ctcagcgacg aaccatgggc ggtacgggca gagtagtagt
      901 cgtagttggc cgtccatacg tcggtgccga acaggttggg cattagcttg gcgagcataa
      961 ccacgtccgc ctgcttgagt atcctgcgat ggcgcgccat gtcctgtatg agagccatat
     1021 cggccgaacg tccagcgtct atcaggtccc gaatgcttcc ggtgatgtcc tcaaggtcaa
     1081 agaatccgtc aaactgctcg atgactttcg tgtcggggtc gggctgcggt atgtacacag
     1141 cctgtgcgac acggcgcatt tcgtcctgct cgcgcaaggt caggccgagt cggcccgaaa
     1201 gctccttcca tgtggacgga tggttggcca tcatccactc cgatgccgac aacgcggcgt
     1261 caatggtcca cttggccatg tagttggtga acgcgttgtt gtcgatgttc tcgtgatact
     1321 cgtccgggcc gatgacgcgc agatactcgt atcggcgcat ctcttcgttc cacgccgcgc
     1381 ggccggccca gaagcgcccg gcctcgatga cgatctcggc gccgtagtct cggaagaagt
     1441 catcgtcgcc ggtccactgc cagtaactcc acacggcatg ggcgacatcg gcggtgatgt
     1501 ggtcttcggt gtcgccgcac cagatcctga tgcgccggcc tgtggcaggg tcgggctttg
     1561 tccaggcggg cgtggtttca tcgccggagt cggcgctttc ccatgcgaac atggcgcccc
     1621 gatacccgcg ctcgcaggcc ttgcgccgcg cgccgggtag ggtgtggtat cgatacatca
     1681 gcaggttccg tgccgcatca gggaacgctg cgcagaagaa cgggtgcacg aacgcttcag
     1741 tgtcccagaa gacgtgaccg cggtaaccct cgccgtgtag tcccttggca ccaatactca
     1801 cccggtcgtc ggtatccggg gcgcacacaa gcatgtggaa tatggcgaac ctcatgccga
     1861 gttggtcata atcaggcccc catatgcgca catcgcaccg actccagcgg gcgacccact
     1921 cgcgacagtg cgccgcaaag agcaggtcgt atccgactcg ggcggctgcc tcagctgatt
     1981 gacacgccgc gtccagcagg gaagatggac ccggtgtcgc aagtggcgca ggcgatgcct
     2041 cgcgagatgc cggttctggt tctgcgcacg cgctcctgac atcgcggccg gttctgtaga
     2101 cagcgtactt ctccaggact agtcgatctc ccgccctcag ataggccctg aattgatggc
     2161 ctgcggcata aggccttcca agcggcgtcg gttccagatg cctccgcgat gcagcgcact
     2221 gcgccgggcc ggcaggatcg gggtcggagt cggaatcggc ggattcgacg aaagccgccg
     2281 aggctagcgc aactgcgatc ggaatatgag agttgatagt acaggcgtcg agccctgcga
     2341 cgccaggtga ggcgccgtgc cgctttggcg aatgcacgcc cagaacgtcc agctgcctca
     2401 agccg
//
LOCUS       DAOTAC010000330         2359 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_34662_length_2359_cov_5.081163, whole genome shotgun sequence.
ACCESSION   DAOTAC010000330 DAOTAC010000000
VERSION     DAOTAC010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34662_length_2359_cov_5.081163"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..1180
                     /locus_tag="PLM74_11110"
     CDS             164..1180
                     /locus_tag="PLM74_11110"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HON43657.1"
                     /translation="MNLKDIARIAGVSTATVSRVINRSGPVSAKTAQKVLEVIEESGY
                     VPDTIAKSLRIGQTKTVGFVVPDISNPFFPEVLNGAEAVCADYGYTIILGNTNEDVAC
                     EAKVVRSLRQQRADGLLMILVDESGGTLRQALSGADSDLPIVFIDRHIEGFHYDSVII
                     DNESGTSQATKHLLGLGHTRIAIIHGPITTTPGAGRLRGYQTAMEAACVPAKPQYVLE
                     GDFRTESGYELAKRLMSLQDPPTAIIGGNNLMTMGIFRCLRDMGVAIPDQVSLIGFDD
                     FSLAGYLSPPLTVVDRPMSDMGRLAADILLSRIEKRETGPVRRVVLPTALKVRGSCRG
                     LNGR"
     gene            1229..1525
                     /locus_tag="PLM74_11115"
     CDS             1229..1525
                     /locus_tag="PLM74_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006874478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HON43658.1"
                     /translation="MIAARVVGTVVCTRKDEKLVGSKLLVVQPVNVLDLSPDGKPLVA
                     VDSVGAGQDEIVLVVSGSSARQTSRTQNTPTDATIMAIVDSIEVQGEWIFRKGI"
     gene            1547..2005
                     /gene="rpiB"
                     /locus_tag="PLM74_11120"
     CDS             1547..2005
                     /gene="rpiB"
                     /locus_tag="PLM74_11120"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HON43659.1"
                     /translation="MRVVIASDHGGFQLKEELKAFIAELGHEVADYGTHTQDPVDYPD
                     LAFLVAEAVAAGAADRGVMIDGIGAASAIVANKVPGIRAASCSDTFTAAMSRAHNDAN
                     VLTLGARVIGSGLAREIVKVWLAAEFEGGRHMRRVSKVLDFEARYLGPRR"
     gene            2013..2297
                     /locus_tag="PLM74_11125"
     CDS             2013..2297
                     /locus_tag="PLM74_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006276525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HON43660.1"
                     /translation="MYTAKVVGHVVATQKDVSLAGAKLLIVVQVDSVGNPVGKPHVAV
                     DAIGAGADEYVTIVRGREASMPLPIPMEPVDAAAVGIVDSMTRTVRGAEQ"
BASE COUNT          499 a          606 c          774 g          480 t
ORIGIN      
        1 acatcaattc catcttggct cggagaaaag cccctacaac tacttgactc agtccaatgg
       61 gggatactct tgacatttcc aattgatgca gtagaatgta atcgaataca ttgaccttga
      121 ccagcgttgc agtggcgagt ggcccgaggc gggaggctgc gacatgaacc tcaaagacat
      181 cgcccggata gctggcgtat ccaccgccac tgtatcgagg gtgatcaacc gcagtggtcc
      241 ggtatctgcc aagactgctc agaaggtcct cgaagttatc gaggaaagcg gctacgtgcc
      301 cgacacgatt gcgaaaagcc tgcgaattgg gcagaccaag actgttgggt ttgttgtgcc
      361 ggacatctcg aacccgttct ttcctgaagt cctaaacgga gccgaagcgg tatgcgccga
      421 ctatggctac actatcatcc ttggcaacac caatgaagat gtcgcctgtg aggcgaaggt
      481 ggttcgtagc ctgaggcaac agagggccga cggactcctg atgatactgg tggatgagtc
      541 aggcggcact cttaggcagg cactttcggg ggcggactcg gatctgccca tcgtcttcat
      601 agacagacac atcgagggat tccactacga ctcagtgata atcgacaatg aatccggcac
      661 ttcgcaggca acgaagcatc tgctcggact gggccatacc aggatagcca ttatccatgg
      721 accgataacc accacgccgg gcgccgggcg gctcaggggc tatcagacgg ccatggaggc
      781 cgcatgcgtt ccggcgaaac ctcaatacgt gcttgaaggc gacttccgaa ccgagtccgg
      841 gtatgagctt gcgaagcgat tgatgtccct tcaggatccg cctaccgcca tcataggcgg
      901 gaacaacttg atgaccatgg gcatctttcg gtgcctgcgt gacatgggag tggccatacc
      961 ggaccaggta tcgctgatcg ggttcgacga tttttcgctc gccgggtacc tcagtccgcc
     1021 tcttacggtg gtggataggc ccatgtccga tatggggcga ttggctgccg atattcttct
     1081 ctcgcgcatc gagaagcgcg aaacggggcc tgtgcgaagg gtggtcctgc cgacggcgct
     1141 caaggtgagg ggttcttgca ggggcctcaa tgggcggtga cttgcaggac gggcacgtgg
     1201 cgagtcatgt atcggaggtg aaggcttctt gatagctgct cgtgttgtcg gaaccgtggt
     1261 atgtacgcgc aaggacgaaa agctggtcgg gtcgaagctt ctcgttgtac agccggtcaa
     1321 tgtgcttgat ttgtcgccgg acgggaaacc ccttgtggct gtggacagtg tgggcgcggg
     1381 ccaggacgag atcgtgctgg ttgtgtcggg cagttcggcc cggcagacat cgcgcacgca
     1441 gaacactcct acagacgcta cgattatggc gatagtagac tccatagagg ttcagggcga
     1501 gtggatcttc cgtaagggca tctgagcaga agggaggcaa ctccggatga gagttgtgat
     1561 cgcctccgac cacggcggat ttcagctgaa ggaagagctg aaagccttca ttgccgagct
     1621 cggccacgaa gtggcggatt acggcactca tacccaggat ccagtggact atcccgacct
     1681 tgccttcctc gttgccgagg cggtggcggc gggagccgca gaccgggggg tcatgatcga
     1741 cgggataggg gcggcgtccg caatcgtggc caacaaggtg cccggcatca gggcggctag
     1801 ctgcagtgat acgttcacgg ccgcgatgag ccgggcgcat aacgatgcta atgttttgac
     1861 actgggcgcg agggtcatcg gctcagggct ggcgcgggag atagtcaagg tttggcttgc
     1921 cgccgagttc gagggcggaa ggcacatgcg aagggtgtcc aaggtactgg attttgaggc
     1981 caggtacctt ggtccgcgga ggtgacgcct gattgtacac ggctaaggtc gtcgggcacg
     2041 tggtagcaac tcagaaggat gtgagccttg caggagcgaa actcctcatc gttgtgcagg
     2101 tcgactcggt gggcaatcct gtcgggaagc cgcatgtggc tgtggacgca atcggcgcag
     2161 gggcggacga gtatgtaacg attgtgcgcg gtcgggaagc cagtatgccg ctgccgattc
     2221 ccatggagcc ggtggatgcg gcagctgtcg ggatcgtgga ttccatgact cgtactgtgc
     2281 gaggtgcaga acagtgaacc tggcaaaggt agtgggaacc atagttgcga cgcagaagaa
     2341 tcccagtttc tccgggtgc
//
LOCUS       DAOTAC010000331         2342 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_34968_length_2342_cov_3.334499, whole genome shotgun sequence.
ACCESSION   DAOTAC010000331 DAOTAC010000000
VERSION     DAOTAC010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34968_length_2342_cov_3.334499"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="PLM74_11130"
     CDS             complement(<1..393)
                     /locus_tag="PLM74_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43661.1"
                     /translation="MGNTSYRLENPSGQDGDEGSQAPGGRERAVLVGLDSTSLSGWDI
                     EESMDELAHLVETAGGQVVARVVQRKQKPDPAHYVGEGKAREVEMMREATGADLVVFD
                     DELSPAQQRNLEEIVKSRVIDRTQLILDI"
     gene            complement(455..1225)
                     /locus_tag="PLM74_11135"
     CDS             complement(455..1225)
                     /locus_tag="PLM74_11135"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HON43662.1"
                     /translation="MILAIDVGNTNTVIGVFCGDKLCADYRVATSREKTADEWAMLIM
                     QLLEFGKIPTSEIAGIILSSVVPPVITVFEKMARKYFGCTADVVGPGTKTGMAIKYEN
                     PKEVGADRIVNAVAAYELYKGPLVIVDFGTATTFCAVTEAGEYLGGVIAPGIMISAEA
                     LYARAARLPRVELVKPQSVIGRNTVASMQSGVIFGFVGQVDEIVRRMKEELHGEPKVI
                     ATGGLAELIAGESSQIDEVNPYLTLIGLRILWDRNHAS"
     gene            1321..2061
                     /locus_tag="PLM74_11140"
     CDS             1321..2061
                     /locus_tag="PLM74_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HON43663.1"
                     /translation="MQALLDVTIVGLAAGVVGTGVGALAIAVAGTPTRRSLSIMMGLA
                     GGVMLSVVFLDLVPEAVEIGGTAYALGGFVLGVGATGIVDLLTPHFHFVSGDCERARY
                     LRAGILTGIGIAMHNLPEGLAIGASYAHGSSLGFSVGLTIALHNIPEGMAMAAPMCAA
                     RLDALQITKWGALAGLPMALGALVGVAVGNVSSAVLSVCLGFAGGAMVFITADELIPD
                     AQEFAVGHSGTFGIVLGVLAGMILVYAL"
     gene            2076..>2342
                     /locus_tag="PLM74_11145"
     CDS             2076..>2342
                     /locus_tag="PLM74_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43664.1"
                     /translation="MSSIDVRRVTRTAILIALTIAFQSLGLQQMVTGPVVNMFLYLAV
                     MTVGIWGGVATGLVTPTVALWVGILKPAMAPMVLGHEAAGVVEEI"
BASE COUNT          432 a          713 c          683 g          514 t
ORIGIN      
        1 tatgtccaga atcagctgtg tgcgatcgat gaccctggac ttcacgatct cctcgaggtt
       61 acgctgctgt gcagggctca gctcatcgtc aaacactacc aggtccgcgc ctgtagcctc
      121 acgcatcatc tcgacttctc gcgccttgcc ctcgcccacg tagtgagcgg ggtcaggttt
      181 ctgtttgcgt tgaacgaccc tggccactac ctgcccgccg gctgtctcga ccaagtgagc
      241 cagctcgtcc atggattctt caatatccca gcctgatagg gacgtagagt cgagcccaac
      301 caggacggcc cgctcacggc ctccgggcgc ctgcgacccc tcgtctccgt cctgcccgga
      361 cgggttctca agcctatatg acgtgttgcc caatctctca tctctctcct gtcgtttcat
      421 gccggtccgc gcaggcgtcc cggcccctat cgaatcatga cgcgtggttt ctatcccaca
      481 gaatacgcag gccaatcagg gtcagatatg ggttgacctc atcgatctga cttgattcgc
      541 cggcgatcag ttccgccagg ccgccggtgg cgattacctt gggttccccg tgcagctcct
      601 ctttcatcct ccgcacgatc tcgtcaacct ggcccacgaa cccgaatatt acccccgact
      661 gcatgctggc gacagtgttc ctgcctatca cggactgagg cttaacaagc tccacgcggg
      721 gcaaacgcgc agcgcgggca tatagcgcct ctgccgatat cattatcccc ggcgcgatga
      781 ccccgccgag atattcccct gcctcggtga cagcgcagaa ggtagtggca gtgccgaagt
      841 ccacaatcac aagcggacct ttatacagct cgtatgcggc aactgcattg acgatacggt
      901 cggctcccac ttccttgggg ttctcgtact tgattgccat gcctgtcttc gtacccgggc
      961 cgaccacatc ggccgtgcag ccgaaatact tccgcgccat cttctcgaat accgtgatga
     1021 ctggaggcac aaccgacgac agaatgatcc cggcgatctc agatgtcggt atcttgccga
     1081 actccagcaa ctgcatgatg agcatcgccc actcgtcagc agtcttctct ctagaagtgg
     1141 ctacccggta gtcggcgcat agcttatcgc cgcagaacac gcctatgact gtgttggtgt
     1201 ttccaacgtc aattgccaga atcatgggac cgcccccccg gcgcaattat accatgtggg
     1261 ctggtctttg acagtgcggc cgtgacagat tagaataggc tggggactgg aggtgaaggc
     1321 gtgcaggcac tacttgatgt aacaatcgta ggtctcgccg caggtgtagt aggtaccggc
     1381 gtcggcgcgc tcgccatcgc ggtggcgggt acacccacca gacgctctct ttcgatcatg
     1441 atgggcctcg ccggaggcgt catgctctct gtggtcttcc ttgatctcgt gcctgaagcg
     1501 gtcgaaatag gaggaacagc ctacgcatta ggcggcttcg tgctcggggt cggggcgaca
     1561 ggaatcgtgg atctactgac gccgcacttc catttcgtct cgggagattg tgagagagca
     1621 cgttatctcc gtgcgggcat actcacagga ataggcatcg ccatgcacaa cctgcccgaa
     1681 ggacttgcca taggcgcaag ttacgcccac ggatcgtcac tcgggttttc cgtggggctc
     1741 accatagctt tgcacaacat tccggaagga atggccatgg ccgcaccgat gtgcgcagcc
     1801 cgtcttgacg ccctgcagat tacaaagtgg ggcgcccttg ccggcctgcc catggcgctg
     1861 ggcgccctgg tgggagtggc ggtcggaaac gtgtccagcg cggtgctttc ggtgtgcctg
     1921 gggtttgccg gcggagcgat ggtcttcata accgccgacg agctgatccc ggacgctcag
     1981 gagttcgcag tggggcattc ggggaccttc ggaattgtcc ttggagttct ggcaggaatg
     2041 atcctggtgt atgcgctgta gaaggaggag acggcatgtc gtcaatagat gttcggcgag
     2101 tgaccagaac ggcgattctt atagctctga ccatagcctt tcagtcgctg ggcctacagc
     2161 agatggtcac gggtccggta gtcaacatgt ttctgtacct ggcggtcatg actgtcggca
     2221 tctggggcgg cgtggctaca ggcctcgtga caccgacggt agcgctctgg gtcggcatac
     2281 tgaagccggc catggcgccg atggtcctcg gccacgaggc ggctggcgtg gtcgaggaga
     2341 tc
//
LOCUS       DAOTAC010000332         2307 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_35616_length_2307_cov_4.866785, whole genome shotgun sequence.
ACCESSION   DAOTAC010000332 DAOTAC010000000
VERSION     DAOTAC010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35616_length_2307_cov_4.866785"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="PLM74_11150"
     CDS             <1..780
                     /locus_tag="PLM74_11150"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HON43665.1"
                     /translation="RLVFAGLAFAAVLLAALCGLFAAKRRTRAVPDSDPGDEPVSNRD
                     VIRSRDLWLLALFLFAKYGSQVAFQGLWGAPYIASVYGVTPTTAATAVTMIAAGYVVA
                     APFIGRLADIMAARGMNLMAAQRRLLIATTLLYVATWVPIVLAPGLLPLGAMYVLLFV
                     MGMSVSSAGLVFGIAKDLFPAKVSGLAIGLVNITSILGGAAMPPVVGWFIRRLTAAGI
                     QGGAVYSGAMIPCLLAACVGLILLSMVGKPSGRRLRPGYPA"
     gene            complement(867..1703)
                     /locus_tag="PLM74_11155"
     CDS             complement(867..1703)
                     /locus_tag="PLM74_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43666.1"
                     /translation="MKRYLLVLGLALLLVSAWAGLAGAAELGLGGKIVYQTGNYAFTI
                     LETGETDDTTRYESSVSAPPLRFELGVTSGAWNLNGFLTTKSFGEAELDPDLLGDADK
                     DCGGVTAFGGDLAYRFDVGSFKLAPSIGYRSMQTTIGQKDSSEGEVHAWGRGLRTRGV
                     RLGAEASATLSDRFSFSASLGCSPRLAVAEIFEIANDFADESVPYRHYIVFDVSKPDK
                     AYSFDLAISAAARIIPAVEIVGGYRYEGIRVELGTSASEFWASGYAVNASVFHIGAEC
                     RF"
     gene            1995..>2307
                     /locus_tag="PLM74_11160"
     CDS             1995..>2307
                     /locus_tag="PLM74_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HON43667.1"
                     /translation="MRVTVGVVRIALGLAVIFALVSAPASPSSVSAGDVEALVRGNNA
                     FAVGLYSAVSYRDGNLFISPYSISSALAMTYAGARGNTAAQMAHALRYDLAPEQLHES
                     FS"
BASE COUNT          441 a          680 c          696 g          490 t
ORIGIN      
        1 aggttggtct tcgcagggct ggcgtttgcg gcagtgctcc tggccgctct gtgcgggcta
       61 ttcgctgcga aacgacgaac gcgcgcagtg ccggattccg acccaggcga cgagccggta
      121 tccaaccgag atgtgatacg cagtcgcgat ctgtggctgc ttgcgttgtt tctatttgcg
      181 aaatacggct ctcaagtggc gttccaaggc ctgtggggag cgccctacat cgcgagcgtc
      241 tatggtgtga cccctactac ggctgctact gccgtgacga tgattgctgc cggttatgtc
      301 gtggccgccc cgttcattgg cagactcgcc gacatcatgg ccgcgcgggg catgaacctc
      361 atggctgccc agcggcgcct gctcatagcc accacactgc tgtacgtggc aacgtgggta
      421 ccaatcgtcc tcgccccggg gctgttgccc ttgggcgcca tgtacgtgct gctgtttgtc
      481 atgggcatgt cggtcagctc ggccggcctg gtatttggaa tcgccaaaga cctgttcccg
      541 gcgaaggtgt cggggctggc gataggcctt gtgaacatca cgtccattct gggcggagcc
      601 gcgatgcctc ccgttgtggg ctggttcata aggcgcctaa cggctgcggg tattcagggc
      661 ggagcggtgt attcaggggc gatgatccca tgcctgctgg ctgcctgtgt gggactgatc
      721 ctgctgtcga tggtggggaa gccttccggg cggcgcctgc ggcctggcta tcctgcgtag
      781 tcgcgtttgc tttgttggta atgggcacgc gcactcgagg cgcccggggc caggggccaa
      841 gccctccccg ggcgccggag actccattag aacctgcatt ctgctccaat atggaatacg
      901 ctcgcgttca ccgcatagcc ggaggcccaa aattcgctgg ccgaggtacc gagctcgacg
      961 cgaatgccct cgtaccggta cccgccgacg atctcgactg cggggatgat gcgagctgcc
     1021 gcactgatcg ctaggtcgaa cgagtatgcc ttatcgggtt tcgaaacgtc gaagacgata
     1081 taatgacggt aaggaacgct ctcatctgcg aaatcgttcg ctatctcgaa gatctccgcg
     1141 actgcgagac ggggggagca tccaagggaa gcggagaacg agaacctgtc gctcagggtg
     1201 gcggatgctt cggctccgag cctgactccg cgggttcgaa gccctctgcc ccaggcatgg
     1261 acttctcctt cagaagaatc cttttgccct atagtagtct gcatgctccg gtagccgatc
     1321 gacggggcaa gcttgaacga ccctacatcg aatcgatagg ctaggtctcc tccgaaagct
     1381 gtcactcctc cgcagtcttt atccgcatcg cctaacaggt caggatccag ctcggcctct
     1441 ccgaagctct tcgtggtcag aaaaccattc agattccacg ctccggatgt tacgcccagc
     1501 tcgaacctga gcggcggagc agaaacagag gactcatatc gagtcgtgtc gtcagtctcg
     1561 ccagtctcaa gaattgtgaa ggcgtagtta cccgtctgat agacgatctt gccaccgagc
     1621 cctaactccg ctgcgccagc caaacctgcc catgcagata ctagcagtag cgcgagccca
     1681 agaaccagca gatatctctt catccaactc ttcctttcag ggcctctcgg cccagtacat
     1741 gggcacaaag gtcagttcta cgtgcggggg ctccactcct tccagcctga gacgcggaga
     1801 cgaagtagta ggattatcgg cgctatccta tatgacttaa gacagtacgg cacggcagca
     1861 ggaaacgcct gatggaatcc gaactgtaca gggcaggcgc cccgggctca gcttcggatc
     1921 gagtcgaata gcgtacatga tgacgcactc cagcgttgat gcatcatcac atctcatgaa
     1981 ggaggcaatg gcaaatgaga gttacggtag gggtagtgcg aatagctctg gggctggccg
     2041 ttatctttgc tctggtcagt gccccggcga gcccgtcctc ggtgagcgcc ggggatgtgg
     2101 aggcattagt gagggggaac aacgctttcg cggtcggcct ctattcggcc gtgtcgtatc
     2161 gagacggcaa cctgttcatc tcgccataca gcatttcttc agcattggcc atgacctatg
     2221 caggcgcgag gggcaacaca gcggcacaga tggcacatgc cctccgctac gatctggccc
     2281 ccgagcagct tcacgagtcg ttctctg
//
LOCUS       DAOTAC010000333         2306 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_35641_length_2306_cov_4.246557, whole genome shotgun sequence.
ACCESSION   DAOTAC010000333 DAOTAC010000000
VERSION     DAOTAC010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35641_length_2306_cov_4.246557"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="PLM74_11165"
     CDS             complement(<1..720)
                     /locus_tag="PLM74_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HON43668.1"
                     /translation="MSTFWGFPRADGKVGSRNYVVVIPTVVCANEVVDAIVRQAQMTR
                     GIMHHQGCCQLPPDLTRVTDALIGIGKNPNAGAVLLVSLGCEGVDVDRVFAEISASGK
                     PVDKVTIQEVGGVTRAIQLGTDIVRKMAEKLGSLQRVETPLSEFVMGIKCGASDATSG
                     LSANPVIGCVADRIIDCGGTVIFGETTEFIGAEHILRRRAVSPAVADRITDIVNRMEH
                     RAMSMGVDMRSGQPTPGNIRGG"
     gene            complement(722..1030)
                     /locus_tag="PLM74_11170"
     CDS             complement(722..1030)
                     /locus_tag="PLM74_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HON43669.1"
                     /translation="MESKKALKIDVIDNVATLLDDAHSDDIVCVYDKGGQAVATVASR
                     SSIAFGHKIALCGMDVGTAVVKYGETIGRASGKISVGDHVHIHNMDSARGRGDLDGGD
                     "
     gene            complement(1048..2016)
                     /locus_tag="PLM74_11175"
     CDS             complement(1048..2016)
                     /locus_tag="PLM74_11175"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HON43670.1"
                     /translation="MSFRVFVTSSLYAAKNKDAIDMMTDAGLDVAFSDLARAHSEHEL
                     IERAHDADGLLVFNTFDAVTRRAIQSCPKLKVISRHGIGLDNIDTAAAAEHGVIVRNT
                     INAQEDEAVADLAFGLVICCARNILRFSLETKAGAWDRHSSQDVSGKALGIVGLGKIG
                     RAMARRARAFNMEVLAYEPFPDTGFCTEYGVRIVELEELLEQSDFVSLHVPATDSTRG
                     MIGAAELALMKPTAYLINAARAQVVDTSALCDVLASGRIAGAGIDVYDSEPPTANPLL
                     NLDNVIATPHVAAYTEQVIRRMDLEAAENVVLTLNPAAVKSSSTGT"
     gene            complement(2052..>2306)
                     /locus_tag="PLM74_11180"
     CDS             complement(2052..>2306)
                     /locus_tag="PLM74_11180"
                     /inference="COORDINATES: protein motif:HMM:NF013353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HON43671.1"
                     /translation="HFGKVLLADLAEFDRDEAEKNVVDAAKRLVERDCDIGAIVLECT
                     NLPPFAKAVQDVTGLPVFDIVTLTNMAYATTLRRVFAGDL"
BASE COUNT          446 a          761 c          571 g          528 t
ORIGIN      
        1 cccgcctcgg atgttgcctg gcgtgggctg tccgctacgc atatcgaccc ccatagacat
       61 cgctcgatgt tccattctgt tgacaatatc agtgatccta tctgcgaccg cgggcgagac
      121 ggctcttcga cgcaatatgt gctctgctcc gatgaactca gtcgtctctc caaagataac
      181 tgtgcctccg caatcgatga tcctatcggc cacacagccg ataaccgggt tggctgagag
      241 cccggatgtc gcatccgagg cgccgcactt tatccccatc acgaactctg acagcggggt
      301 ttctactctc tgcagagacc ccagtttctc ggccatcttc cgcacaatgt ccgttccaag
      361 ctgtatcgca cgcgtgacgc cgcccacttc ctggattgta accttgtcca ctggttttcc
      421 agacgcggag atctcggcga acacccggtc cacgtccact ccctcgcacc ccaggctgac
      481 cagaagaact gcgccggcat tggggttctt gcctattcct atgagcgcgt ccgtgaccct
      541 cgtcaagtcc ggcggaagtt ggcaacaccc ttgatggtgc atgatccccc tggtcatctg
      601 agcctgacgc acaatcgcgt ccaccacctc gtttgcacac accacagtag ggatcacgac
      661 cacgtagttc ctcgatccga ccttcccatc ggcccgagga aagccccaga acgtgctcat
      721 cctaatcccc tccatccagg tcgccgcggc cccgcgcgct atccatgttg tggatatgaa
      781 catgatctcc cacggatatc ttgccgcttg ccctgccaat cgtctcaccg tacttgacta
      841 cggcagttcc tacgtccatg ccgcatagcg cgatcttatg gccaaacgcg attgagctgc
      901 ggcttgcgac agtcgccact gcttggccac ccttgtcgta tacacacact atgtcatcgg
      961 aatgcgcatc atccaacaag gtagctacat tgtcgatgac gtctatcttg agggccttct
     1021 tcgactccaa ctgcctcgcc tcccgactta cgtgcctgtg cttgatgatt tgaccgctgc
     1081 tggattcagc gtcaatacca cgttctctgc tgcttccaga tccattctgc gtattacctg
     1141 ctcggtatag gccgcgacat gaggggtcgc gattacgttg tccagattga gcagtggatt
     1201 ggccgtcgga ggttcgctat catacacatc gattccggcc cccgctatcc gcccggaggc
     1261 aagcacatcg cacagcgcac ttgtgtcgac cacttgagct ctggcggcgt tgatcaagta
     1321 cgccgtcggc ttcatcaggg ctaactccgc cgctccgatc atgccccggg ttgagtcagt
     1381 cgccggcaca tgcaagctga cgaagtcgga ctgctcgagc aactcctcca actccacaat
     1441 tctcacgcca tactcggtgc agaaccccgt gtccggaaat ggttcgtacg ccaagacttc
     1501 catgttgaag gcacgggccc ttcttgccat cgcccgtcct atcttgccga ggcccacgat
     1561 accgagcgcc ttgccgctca catcctgcga gctgtggcga tcccaagccc ccgccttggt
     1621 ttcgaggctg aagcgcaata tgttgcgggc acaacatatc accaagccaa acgccagatc
     1681 ggcgacggct tcgtcttctt gggcgttgat tgtgttcctt actatcacac cgtgttccgc
     1741 tgcggcagcc gtgtcgatgt tatccagccc tattccgtgc ctggagatca ccttgagctt
     1801 tgggcagctc tgtatggctc tcctcgtgac agcgtcaaac gtgttgaaca ccaagagccc
     1861 atcggcgtcg tgtgcgcgct ctatcaactc atgctcactg tgcgcgcggg cgagatcgct
     1921 gaacgctacg tctagccccg catctgtcat catgtctatg gcgtctttgt tcttggcggc
     1981 gtacaagctg gacgtgacaa agaccctaaa gctcaacctg ttgaccctcc ttccaggcat
     2041 caagaacggc tctacagatc gccggcgaag acccttctca gcgtagtcgc gtacgccata
     2101 ttggtaagag tgactatatc gaaaaccgga agacccgtaa cgtcctgcac cgccttggcg
     2161 aagggcggca ggttggtgca ctccaggact atcgccccga tatcacaatc tctctccacc
     2221 agcctcttcg cagcgtcgac cacattcttc tcagcctcgt cccggtcgaa ttcggcaagg
     2281 tcggcgagga ggaccttgcc gaagtg
//
LOCUS       DAOTAC010000334         2250 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_36764_length_2250_cov_5.158998, whole genome shotgun sequence.
ACCESSION   DAOTAC010000334 DAOTAC010000000
VERSION     DAOTAC010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36764_length_2250_cov_5.158998"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1351
                     /gene="lon"
                     /locus_tag="PLM74_11185"
     CDS             <1..1351
                     /gene="lon"
                     /locus_tag="PLM74_11185"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HON43672.1"
                     /translation="GKSIARALGRKFVRFSLGGVRDEAEIRGHRRTYVGAMPGKIVQL
                     MKQAGSMNPVILLDEVDKMSSDFRGDPASALLEVLDPEQNREFGDHYMEVTVDLSDVL
                     FITTANNLYSIPRPLQDRMEIIGIPGYTEEDKLHIASKFLIPKQVRENGLAEGHVQMD
                     DDVIREIIRSYTRESGVRSLERQIGSVCRKLAREVVAGASGPFVVNRADLAKHLGIPQ
                     FRYGVAEQDDKVGVATGVAWTETGGDTMSIEVSAMKGGGKLILTGKLGEVMRESAQAG
                     YTYVRSRARDLGIPDDFYEKMDVHIHVPEGAIPKDGPSAGITMATALASALSGVSVRH
                     DVAMTGEITLRGRVLPVGGLKEKVLAAHRAGITTMIIPEDNRKDLEEIPENVRADIAF
                     VEVAHMDEVLAVALARYGASDTDSLRDDLDSGEDDVHAAVMVPPANPPSTHQPPAAL"
     gene            1626..>2250
                     /locus_tag="PLM74_11190"
     CDS             1626..>2250
                     /locus_tag="PLM74_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HON43673.1"
                     /translation="MKRIKVIIMGAAGRDFHNFNVYFRDNEKYDVIAFTATQIPDIAG
                     RKYPAVLAGGLYPEGIPIFPEEDLVKLISEHRVEQVVFAYSDIAHIDIMHKASMVNAA
                     GADFRMMGPTTTMIKSKAPVVAICAVRTGVGKSQTTRYVSQALIDAGLKVVAIRHPMP
                     YGDLAQQAVQRFATYDDLDLHECTIEEREEYEPHIDRGVIVYAGVDYG"
BASE COUNT          508 a          613 c          711 g          418 t
ORIGIN      
        1 tggcaaatcg attgcccgcg ccttgggccg caagttcgtc cggttctcgc tgggcggcgt
       61 gcgagacgag gctgagatca gaggacacag gcgtacttat gtaggcgcta tgcccggcaa
      121 gatagttcag ctaatgaaac aggcgggcag catgaacccg gtcatacttc tggatgaagt
      181 ggacaagatg tcgtcggatt ttcgaggcga tccggcttcg gctctgctcg aagtgctcga
      241 ccctgagcag aaccgcgagt tcggcgacca ttacatggag gtcacagttg acctgtccga
      301 cgtgctgttc ataaccacgg ccaacaatct ctattccata ccccgtccat tgcaggacag
      361 gatggagata atcggcatac cgggctacac tgaggaggac aaactccaca tagcgtccaa
      421 gttcctcata cccaagcagg tccgcgagaa cggactggcc gaagggcacg ttcagatgga
      481 cgacgacgta atcagggaga tcatccgctc atataccagg gagtcggggg tacgcagcct
      541 tgagaggcag atcggctcgg tctgccgcaa gctggcccgc gaggttgtag ccggggcgag
      601 tggcccgttc gttgtgaacc gggccgacct tgcgaaacac ctggggatcc cgcaattccg
      661 gtacggcgtg gccgagcagg atgacaaagt gggtgtggcc accggcgtgg cctggaccga
      721 aaccggcggc gataccatgt ctattgaggt gtcggcaatg aagggcggag gcaagctgat
      781 cctcaccggc aagctgggag aggtcatgcg cgaatccgca caggccgggt acacgtacgt
      841 caggtccagg gcgcgcgatc tgggcatacc cgacgacttc tacgagaaga tggatgtgca
      901 catccacgtc ccggagggcg ccatccccaa agatggcccg tcggcaggga taaccatggc
      961 gaccgcgctg gcatcggctc tgtcaggcgt atcagtcagg catgatgtgg ccatgaccgg
     1021 ggagataaca cttcgcgggc gagttctgcc tgtaggcggg ctcaaggaga aagttcttgc
     1081 cgcgcatagg gcaggaatta cgaccatgat catccctgaa gacaaccgca aggacctaga
     1141 ggagattcca gagaacgtgc gggccgacat cgctttcgtc gaggtagcgc acatggacga
     1201 ggtgctggcg gtggcgctcg cccgatacgg cgcgagcgac actgatagcc ttcgcgatga
     1261 cttggacagt ggcgaggacg atgttcacgc tgccgtcatg gtgccgccag cgaatccgcc
     1321 atcgacgcac caacccccgg cagcactctg acaagaccga ttcagttgtc aggctgatcc
     1381 gtgaggcgcc cgcaagggcg cctcttctgt tggatgggcg cctgtggagg aattgggcgc
     1441 ttagtagtag aataggtgac gggcgcagta tgcatccatg catattaatg cagccgcgtg
     1501 tattgcgcac atgatcggac ggagcgtgga tggatggtca ggctgccaag tgtgtacgat
     1561 aagcgtgcgt acattgccgc agcgcagcat tggtacttga taacggagaa aggggtagtt
     1621 gcttcttgaa gaggatcaag gtcataatca tgggcgcagc ggggcgcgac ttccacaact
     1681 tcaatgtcta cttccgagac aacgagaagt acgacgtgat tgctttcaca gccacccaga
     1741 tacccgatat tgccggaagg aagtacccgg cagtgctcgc cggcggtctt taccccgagg
     1801 ggataccgat cttccctgaa gaggatcttg tcaagctcat tagtgaacac cgagtggagc
     1861 aagtagtctt cgcatacagc gacattgcgc acatcgatat catgcacaag gcctcaatgg
     1921 tcaacgcagc gggcgccgac tttaggatga tggggcccac cacgaccatg atcaagtcca
     1981 aggcaccggt agttgcgatc tgcgcagtcc gcacaggcgt gggcaagagc cagaccactc
     2041 gttacgtgag ccaagcgctg atcgatgcag gcctgaaggt cgtggcgatc aggcatccga
     2101 tgccatatgg cgacctcgcc cagcaggcgg tccagcgctt cgctacatat gatgacctcg
     2161 atctacatga gtgcacgatc gaggagcgcg aggagtacga acctcatatc gatcgtggcg
     2221 tgatcgtgta tgccggcgtc gactacggca
//
LOCUS       DAOTAC010000335         2211 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_37597_length_2211_cov_4.434601, whole genome shotgun sequence.
ACCESSION   DAOTAC010000335 DAOTAC010000000
VERSION     DAOTAC010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37597_length_2211_cov_4.434601"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2211)
                     /locus_tag="PLM74_11195"
     CDS             complement(<1..>2211)
                     /locus_tag="PLM74_11195"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HON43674.1"
                     /translation="RIRYQLPEGVQDPIVSLIDPSQLPLMMLTVGAQGDLGEVTRVAR
                     EIVKPEIERVDGVAGVSISGGADRVISVEYDHEKLAEAGLSPLLLQQLISLQNLSVPA
                     GVVVDDGVRYQTRVGAKFQSAEEIASLTIGMNTKRSEPESGQPRLGGLMGLSFLMPSF
                     LTTIGDVATVEEKFQKTEGYARVDGNSSVMLLVYKQSGQNTVTTARRVNQALDRLTAG
                     RSDISVAHVFDQSTYINRSINGLRDNLILGAVLAVVVLYIFLRHVGSTLVVAVSIPLS
                     VVFAFVLMYAWKLSVNLMTLGGLALGVGMLVDNSIVVLESIYRRQELGDPPLTAAVNG
                     TREVGTAIVSSTMTTVAVFVPVVFIEGMVGTIFRDLAMTVTFSLLASLAVALTVVPLL
                     ASGLVRLHGKAELKDSVPAPGGTGARSRQTLNSRYDRALTWAMRNRWAVLGFVGAAVV
                     ATVLIYPSLGVELFKNMDMARVDVSVLMPAGTPTDITNQVSMLLEEKFMQMPGLEHVT
                     AEVGSTGGDDFLAMASNAGSNSASISVALRREGPVQTSVSEAAEFIRAAIASVQEEYP
                     ELECTVDTSGYGSLTGGQIDLFGSNVSLEILGSDSREFVEYSSRVAQRLREVPEFVEV
                     ASSAQELQPVILLNVNPTRALMGSMTVGQVGLGVRTAMLGTTVTYVERGGELIPVIVR
                     PASDGVPTLESVLDMPISSSLGASSIMQSASQMGSNAAAAARASGTGTSSLLAP"
BASE COUNT          476 a          708 c          611 g          416 t
ORIGIN      
        1 gcggagcaag gagcgaagaa gtacctgtgc cggacgcgcg cgcggcagcc gcggcattgc
       61 ttcccatctg gcttgcgctt tgcatgatcg aggatgcccc caaagacgag ctgatcggca
      121 tgtccagcac cgattcgagc gtaggaactc catcgctcgc cggcctgaca atgaccggga
      181 tcagttcacc tccacgctcc acatacgtta ccgtagtgcc cagcatggcg gtgcgtaccc
      241 ccagcccgac ctggcccaca gtcatcgacc ccatcagggc gcgcgtagga ttgacattga
      301 gcaagatcac aggctgcagc tcttgtgcag atgatgccac ttctacaaac tcaggcactt
      361 cgcgcagtcg ctgggccacc cgcgatgaat actccacaaa ttcccttgag tccgagccca
      421 gtatctcaag agacacgttg ctgccgaaaa ggtcaatctg tccgcctgta agtgaaccat
      481 acccgcttgt gtctactgtg cactcaagct ctgggtattc ctcctgcacg ctggcgatag
      541 ctgcgcggat gaactcggca gcctcagaaa ccgaggtctg aacaggacct tcacgacgca
      601 gcgcaacgct gatactggcc gaattggacc cggcattgga cgccatggcc aggaagtcat
      661 cgccgcccgt cgaacccacc tcggccgtta cgtgctctag ccccggcatc tgcatgaact
      721 tctcttcaag cagcatcgac acctgattag tgatatcggt aggcgttccg gccggcataa
      781 gcacactcac atccacccgg gccatgtcca tattcttgaa cagctcaacg ccgagactcg
      841 gatagatcag cacggtagcg accacagccg ctcccacgaa cccaagcaca gcccatcggt
      901 tgcgcatcgc ccacgtgagc gctcggtcgt agcggctgtt caacgtctga cgactccggg
      961 cgccggttcc cccgggcgcg ggaactgagt ccttcaactc cgccttgccg tgcaggcgca
     1021 ccagcccgct ggcgagaaga ggcaccaccg tcagggcaac ggccagcgat gccagaaggg
     1081 agaaggtaac ggtcattgcc aggtcccgaa agattgtgcc caccattccc tctatgaaaa
     1141 cgaccgggac aaagactgca acggtagtca tagtggagct gacaatggcg gttccgactt
     1201 cgcgggtgcc gttcacagcc gccgtcagag gcggatcgcc aagctcctga cgcctgtaga
     1261 tgctctccaa caccacaatg gagttgtcca caagcatgcc gacgcccaga gccagcccgc
     1321 ccagggtcat caggttgaca ctgagtttcc aggcgtacat caacacaaag gcaaacacca
     1381 ctgagagcgg aatggacact gcgactacaa gagtgctccc cacatgacga aggaaaatgt
     1441 agagaacgac aacggcgagc actgcgccga ggatcaggtt gtccctgagg ccgttgatgg
     1501 acctgttaat ataggtggac tgatcgaaca catgcgccac cgagatgtcg cttcgtccgg
     1561 cggtgagcct gtcgagagcc tggttgacgc gtctggccgt ggtcaccgtg ttctgacccg
     1621 actgcttgta caccagcaac atcacagacg agttgccgtc cacccgagca tagccttcag
     1681 tcttctggaa cttctcctct acggtggcga catctcctat tgtcgtcagg aaagacggca
     1741 tcagaaacga cagacccatg agaccgccca gccgcggttg gcctgattcg ggttcgcttc
     1801 gcttggtgtt catgcctatc gtaagcgacg cgatctcctc cgccgactgg aacttggcgc
     1861 ccacccgcgt ctgatagcgc accccatcgt cgaccacgac gcctgccggc accgataggt
     1921 tctgcagcga gatgagctgc tgaagcagaa gcggactgag ccctgcctct gccaacttct
     1981 cgtggtcgta ctcgacagag atcactcggt cggccccgcc gctgatagag acgccggcta
     2041 cgccgtcaac tctttcgatc tcaggtttga caatctcgcg ggcgactcga gttacctcgc
     2101 ctaggtctcc ctgtgctccc actgtgagca tcatcagcgg gagctgcgat gggtcgatga
     2161 ggctgacgat agggtcctga acgccttcgg ggagctggta tctgatcctg t
//
LOCUS       DAOTAC010000336         2193 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_37980_length_2193_cov_4.793732, whole genome shotgun sequence.
ACCESSION   DAOTAC010000336 DAOTAC010000000
VERSION     DAOTAC010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37980_length_2193_cov_4.793732"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            191..844
                     /locus_tag="PLM74_11200"
     CDS             191..844
                     /locus_tag="PLM74_11200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HON43675.1"
                     /translation="MPNLADLTDAELVQAFREGRSDRAFEQLLRRYVRLIKSWTRKYW
                     CAYRIADYDDLEQEARIAFLGAVNSYDQAQSGFFGFAQLCVTRRLKSVVKSARRLKNC
                     PPAGTVILSTSSSGSEGDDRDLIEAVPNPAAFDPLNMAVANDAARRLWSIAGSALSGL
                     ELEALKRRAWGESYQEVAAELGVSQKSIDNAVTRARRKLRSLIQSDGEPLMEADESA"
     gene            912..>2193
                     /locus_tag="PLM74_11205"
     CDS             912..>2193
                     /locus_tag="PLM74_11205"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HON43676.1"
                     /translation="MVDDLISSAEVSNAAHDEGNALEAGFRTHRRIRAMLGMLEASEG
                     SCSISHIAAQLGVSARTVYRYISQLQMVGHDIRLEGGEVRSGSGSAPGSAPGSGAESR
                     EVRVGPAYHIESSEPAMRRLAILSLLTGGPATLGRIVSYLADPLRALAADERTVRRDL
                     EHLESAGYVEAPGERNTGYCLSGAFMPRFSLTFPELISVVRALESAPEAVADPRTTAS
                     IRSKLFAAMAPSVDQAHALRRRRYTKGRARRSDPAILARVEALEVASLEGRVVQIMYA
                     ERRKGRRNVREVEPLGLVYYWFHDMWYLVATCCTAREIRHFRVDRIGGIAMTNRLFTY
                     PCEFDLASYVSGMWGVYAGEPTRVRVRFFDEMNVLARLRAEIADRPSARLSQVDRDVW
                     ELVDTVAGLSEFRVWLRSFGSSALVMEPECLRMQL"
BASE COUNT          426 a          612 c          724 g          431 t
ORIGIN      
        1 tccgcgaacc tgtgccctgt gggccagggt ttccgagggg cctgggtaaa ccgacacagg
       61 atggccgaaa aaggtgtccc gtagggtaca ggacccgacg ccggccgctt gtgacaaatc
      121 ggcgccgcac ccccgtgata cgatctcctc ggcagcagcc gaggatcgtt tcattccggg
      181 gggtgttcat gtgccgaatc tggcggatct caccgatgct gaactggtcc aggcattccg
      241 tgaaggcagg tcggatcgcg ctttcgagca actgctcaga aggtatgtac ggctcatcaa
      301 gtcatggaca aggaaatact ggtgcgcgta tcggatcgct gactatgacg acctggagca
      361 agaggccaga atcgcttttc tgggcgccgt caactcatac gaccaggcac aatcgggctt
      421 tttcgggttt gcgcagctgt gtgtcacacg gcgtctcaag agtgtggtga agtcggcgag
      481 gcgcctcaag aactgcccac ccgccggcac agtgatcctg tcgaccagct cgagcggttc
      541 tgagggcgac gatcgtgatc tgattgaggc agtgccaaac cccgccgcgt tcgaccctct
      601 caacatggcc gtggccaacg atgcggcgcg taggctgtgg agtattgccg gctcagccct
      661 ttccgggctg gagctggagg ctctgaagag gcgggcatgg ggagaatcgt accaggaagt
      721 cgctgctgaa ctgggagtta gccagaagag tatcgacaat gcagtgacgc gtgcacggcg
      781 caagcttcgc agcctcattc agtccgatgg ggaaccgctg atggaagcgg acgagagcgc
      841 ttgaccagag gtggtagcga tcgaggagga gcaagtcgct taatgtcgaa tgccgccatg
      901 ggtgatcgac gatggtggat gatctcatat cctcagcaga agtgtcgaac gcggctcacg
      961 atgagggcaa tgcgctggag gccggcttcc gaactcacag gaggatccga gcgatgctgg
     1021 gcatgctcga ggcctcggaa ggcagttgtt cgatttctca catagcggcc caactgggcg
     1081 tgtcggcgcg cacagtctac cgctacataa gccagctaca gatggtgggc cacgatatcc
     1141 gactcgaagg cggcgaagtg cgatcaggat cggggtcagc gcccgggtcg gcgcccgggt
     1201 caggggccga atcgcgggaa gtgcgggtcg gccctgcgta ccatattgag tcgtccgaac
     1261 ccgccatgcg ccgccttgct attctctctc tgctcacggg cgggccggcg acgctcggcc
     1321 gaatcgtgtc gtacctagct gaccccctcc gggcactggc agccgatgag cgcaccgtca
     1381 ggcgcgatct tgagcatctg gagtctgcag gctacgtaga agctccaggt gaacgcaaca
     1441 caggatactg tctgagcggg gcttttatgc cgcggttctc tctgacattt cccgagctga
     1501 tctcagtggt gcgcgcattg gaatcggccc ccgaagctgt agccgacccg cggactacag
     1561 cctccatacg cagtaagctc tttgccgcta tggcccctag tgttgaccag gcccacgctt
     1621 tgaggcgacg caggtacact aagggtaggg ctcgacggtc cgatccggcc atcctggcac
     1681 gagtggaggc tttggaagtc gcgtcgctgg aaggccgtgt agtgcagata atgtacgcgg
     1741 aaaggcgaaa aggccgccgg aatgtccgcg aggttgagcc tttgggcctt gtgtattact
     1801 ggttccacga catgtggtac ctggtggcca catgctgcac ggcacgtgag attcggcact
     1861 tcagggtcga ccgcatcggc gggattgcca tgacgaacag gctgtttacg tacccctgcg
     1921 agttcgacct tgcctcctac gtttcaggta tgtggggcgt gtacgccggc gaacccacgc
     1981 gcgtgagggt gcgattcttc gatgagatga acgtgctcgc ccgcctgcgt gcggagatag
     2041 ccgacaggcc tagcgcccgc ctatcacagg tagacagaga tgtgtgggaa ctggtcgata
     2101 cggtagcagg gctctctgag tttcgggtct ggcttagaag cttcggcagt tcggcactgg
     2161 tgatggagcc cgagtgcctg cgtatgcagc tga
//
LOCUS       DAOTAC010000337         2107 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_39918_length_2107_cov_3.460526, whole genome shotgun sequence.
ACCESSION   DAOTAC010000337 DAOTAC010000000
VERSION     DAOTAC010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39918_length_2107_cov_3.460526"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="PLM74_11210"
     CDS             complement(<1..194)
                     /locus_tag="PLM74_11210"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HON43677.1"
                     /translation="MKKVGFIGLGVMGLPMCRNLMKAGYPVVAFDLKREAVDEVVGEG
                     AERGVSPQDVASRAEVIMTML"
     gene            complement(211..2085)
                     /locus_tag="PLM74_11215"
     CDS             complement(211..2085)
                     /locus_tag="PLM74_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HON43678.1"
                     /translation="MYGYCGRILHVDLTSRRCHVEEPSEDFYRKYIGGSSLGVYYALT
                     KMPPRVDAFDPRNVLVFATSATTGAPISGASRFNVTAKSPLTGAIGDSQAGGFWGTEL
                     KYAGFDAIVITGRSPEPTYLWICDGEYELRDATAMWGTETGEAQRAIREELGDPKVRI
                     ALIGQAGENLVRYACITNELKHFNGRTGMGAVMGSKNLKAIAVRGTRPPQFADKDTIT
                     ALARSGARRSVSHPVAMSLRDYGTAAVVGGNQAASGLPTRNWTSGVFQHADAISGAAM
                     KKTILVKNEACWACAIRCKRVVKADEPYVVDPAYGGPEYESIGALGSYLEIGDLVAVS
                     KATELCNRYGLDTISTGGTLAFAMECYENGIITSDDTGGLELKFGNADAALKMIEAIA
                     LRKGIGDVLAEGSARAAERFGNGARRFAICVKRQEFPAHMPRVKASLALAYACNPFGA
                     DHQSSEHDPCLASEPFDEDVTSLGFASAVAPSTLNFEKVKLWAYSQRLYSALDTLDLC
                     QFCFGLGLLYGPSDMVDLVNAATGWSTTLWELMQAGERRVNMMRIFNEREGFSSEDDV
                     LPERVFEPLQGGASDGNRIDRNEFNEVRRQYYEMVGWCPSTGNPTETKLKELGLEWAL
                     "
BASE COUNT          423 a          669 c          544 g          471 t
ORIGIN      
        1 agcatggtca tgatcacttc tgcccgactc gctacgtcct gaggagacac gccccgctcg
       61 gcgccctcgc caactacttc gtcaactgcc tcacgtttca ggtcaaatgc gactaccggg
      121 tacccggctt tcatgaggtt ccggcacatg ggcaacccca taactcctag tccgatgaag
      181 ccgactttct tcacagcaat tccttcccga tcaaagggcc cattcgagcc caagctcctt
      241 taatttggtc tcggtgggat tcccagtact tgggcaccat ccaaccatct cgtagtactg
      301 acggcgcacc tcgttgaact cattgcggtc tattctgttg ccgtcactgg ctccgccctg
      361 gaggggttca aacacgcgtt ccggcagtac atcatcttcg gagctaaatc cttcacgctc
      421 gttgaagatg cgcatcatgt tcaccctccg ctcgcctgcc tgcatgagct cccacagcgt
      481 agtactccag cccgtagctg cattgaccag atccaccata tccgaaggcc cgtatagcag
      541 tccgaggcca aaacagaact ggcatagatc cagggtatcg agagcgctgt agagtcgctg
      601 agagtatgcc cacagcttga ccttctcaaa gttcagtgtg ctgggcgcaa ctgcgctcgc
      661 aaaacctagg ctagtgacat cctcgtcaaa cggttctgat gccaggcacg ggtcatgttc
      721 cgaagattgg tgatctgctc caaatggatt gcacgcatac gccagcgcca gcgaagcctt
      781 gacgcgaggc atgtgcgcgg gaaactcctg ccgcttcaca cagatcgcaa atcggcgagc
      841 gccattgccg aagcgctcgg ctgcccttgc cgaaccttca gccaggacgt cgccaatgcc
      901 cttgcgcagt gctattgcct ctatcatttt gagagccgcg tctgcgtttc cgaatttgag
      961 ttccagtccg ccagtgtcat ctgaggtgat tatcccgttc tcgtagcact ccatggcgaa
     1021 agccagagtg cctcctgtcg atatggtgtc gagtccgtat ctgttacata gctcggttgc
     1081 tttcgacaca gcgacaaggt cgccaatctc tagatacgaa ccgagggcgc cgatagactc
     1141 atattccggc ccgccatagg ccgggtcgac cacgtaaggt tcgtcggcct tgacgactct
     1201 cttgcacctg atggcacacg cccaacaggc ctcgttcttg accagaatcg tcttcttcat
     1261 cgcggcgccc gaaatggcgt cggcatgctg gaatacgccg gatgtccagt tccgcgtcgg
     1321 aagcccagac gcggcttgat ttccgccgac aacggccgcg gtgccgtagt cgcgcagact
     1381 catggccacc ggatgactca ccgaccgcct agctcccgac ctagccagtg cagtgatcgt
     1441 atccttatcg gcaaactggg gcgggcgtgt acctcgcaca gctatggcct tgaggttctt
     1501 ggaccccatg actgcaccca tgccagttcg cccgttgaaa tgcttgagtt cattcgtaat
     1561 gcaggcgtat ctgacaaggt tctcgccggc ttgaccgatc agcgcgatcc tgaccttggg
     1621 gtccccgagc tcctctctga tggcccgctg agcctcgcct gtttcggtgc cccacatagc
     1681 cgtggcatcc cgcagttcgt actccccatc gcagatccac aggtatgtcg gctcgggaga
     1741 tcggccagta atgacaatgg cgtcaaaacc ggcgtacttc agttctgttc cccagaaccc
     1801 tcctgcctga ctgtcgccta tggcgccggt gagaggcgac ttagcagtga cgttaaaacg
     1861 cgaagctccg cttatcggcg cccctgtagt cgctgaggtt gcgaatacga gcacattgcg
     1921 cggatcaaac gcgtcgactc ggggcggcat tttcgtcaag gcatagtaca caccgagaga
     1981 actacccccg atgtacttgc ggtagaaatc ttcgctcggc tcctccacat gacaccttcg
     2041 gctggtcagg tcgacatgaa ggattctgcc gcagtaccca tacataatgg tatccctcct
     2101 tcgcctg
//
LOCUS       DAOTAC010000338         2073 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_40689_length_2073_cov_4.068385, whole genome shotgun sequence.
ACCESSION   DAOTAC010000338 DAOTAC010000000
VERSION     DAOTAC010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40689_length_2073_cov_4.068385"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="PLM74_11220"
     CDS             complement(<1..195)
                     /locus_tag="PLM74_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON43679.1"
                     /translation="MRHIARLVAISRDYWRWMVIALIAMLGVTAANLTGPWLIRNLIG
                     TIEKGFGHGTAAASQVVDISA"
     gene            333..1739
                     /locus_tag="PLM74_11225"
     CDS             333..1739
                     /locus_tag="PLM74_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HON43680.1"
                     /translation="MLKKINALFGAQDMTVGQPMSALLRFSIPLLIGNLAQQMYSTVD
                     SVIVGRYVGDKALSAIGTTLPIINLLLVLFMAVSTGAGIMVAQYYGAKAKESLSRSIG
                     NAITLVCVSSLVVMAIGIPLSGSLLRLTRTPVETFDMARSYLTIILWGVLGLGFYNIV
                     SGILRGLGNSVFPLLTLLLTSCLNIVLDIWFVAGLDMGVAGAAWATIISVAISAIVCI
                     VKLWRMKDVVQIDAANLIPSKKLTAQLLRLGLPAGITQAVFSMSMVFVQALANSMGYQ
                     VVTCTTAVMRIDGFAMMPNFTFGMAIATFVGQNIGADRMDRVHQGSKDMLKVSLLTSF
                     VLVASLLVFGKYLISMFTTTEEIINLGVRQIRILAAGYVAMAITQVYGGIMRGAGDTM
                     PSMWISMFTTVVVRVPLAYLWAWLTRSQAYPAGSPDALFFALLVSWTVGAAATYLWYR
                     RGTWRDKSLVRSSAPLTA"
     gene            complement(1849..>2073)
                     /locus_tag="PLM74_11230"
     CDS             complement(1849..>2073)
                     /locus_tag="PLM74_11230"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HON43681.1"
                     /translation="RGYQPFEWKGEGFFIDISSRVKAVCGAPVIVTGGIKRPATADAI
                     IRQSKADLVGIGRALLSDPDWAVKARLALG"
BASE COUNT          399 a          597 c          602 g          475 t
ORIGIN      
        1 ggccgatatg tcgaccacct ggctcgccgc tgccgtcccg tggccgaagc ctttctcaat
       61 tgtgccgatg aggtttctta tgagccaggg gcctgtgaga ttcgcggcag tcactcccag
      121 cattgcgatg agagctatca ccatccagcg ccagtagtca cgtgatatcg ctacgagacg
      181 ggctatgtgt cgcaaaaccg agtcacctca tcatgagtgt tcgacctttt gggaagacct
      241 cctgcgaagc agcccgccgg ctgcgtatca ctgcgcatat tgcactgtgg gcgtctacgt
      301 gacataatca tcgcagaaaa ggtggttgct atatgctcaa gaagatcaat gcactgttcg
      361 gcgcccaaga catgaccgta gggcagccga tgtctgcctt actgaggttc tccatccccc
      421 tgcttatcgg caaccttgcg cagcagatgt acagcacggt ggattcggtc attgtaggtc
      481 gttacgtggg tgacaaggcc ctttcggcca tcggcacgac gctcccaatc atcaacctgc
      541 tccttgtgct gttcatggca gtctcgacag gcgcaggaat catggtggcg caatactacg
      601 gggctaaggc caaagaatcg ctgtcgcgtt cgattggcaa tgcgattacc ctggtctgcg
      661 tgtcgtcact tgtggtcatg gcgatcggta tacccctgtc gggttcgctg cttaggctga
      721 cacggacgcc tgtggagacg ttcgatatgg cccgatccta ccttactatt atcctttggg
      781 gagtcttggg gctcgggttc tacaacatag tatcaggaat cttgagggga ctgggcaatt
      841 ctgtctttcc gctgctgact ctgttgctca catcatgtct gaacatagtc ctggacatct
      901 ggtttgtggc aggcctggac atgggagtcg caggcgcggc ctgggcaacg atcatctccg
      961 tagctatctc agccattgta tgcatcgtta agctgtggag aatgaaggac gtcgtgcaga
     1021 tagacgcggc gaatctgatc cccagcaaga aactgaccgc ccagctgttg cggttggggc
     1081 tgccggcggg cattacacag gcggtcttct caatgtctat ggtgttcgtg caggcgctgg
     1141 ccaacagcat gggctatcag gtggtcacct gtaccacggc agtcatgcgc atcgacgggt
     1201 tcgcaatgat gcccaacttc accttcggca tggctatcgc caccttcgtc ggccagaata
     1261 ttggggcgga caggatggac cgtgttcacc agggatcgaa agacatgctg aaagtgagct
     1321 tgctcacctc gttcgtgctg gtggcgtcat tgttggtatt cggcaagtac ctgatcagca
     1381 tgtttaccac cacggaagag attatcaacc tgggcgtaag gcagatccgc atactggccg
     1441 ccggctatgt ggccatggcg atcacccagg tatatggcgg gatcatgcgc ggtgcgggcg
     1501 acaccatgcc gtccatgtgg atttcgatgt ttactacggt cgtggtacgc gtgcccctgg
     1561 cgtatctttg ggcatggctc accaggagcc aggcgtaccc tgcaggatcg cctgacgcgc
     1621 tgttcttcgc gcttctcgta agctggaccg taggggctgc cgccacctac ctctggtacc
     1681 gccggggaac ctggcgagat aagtcgctag tacggtcgtc agcgccgttg acggcgtagg
     1741 cccaaggcac atcggcgtag ttggcagaca aacacctgta gcgccggcgt tctccgacga
     1801 cgcaggtcct tgcccaaatc accgcacctc atggcctccg acccccggct agcccagcgc
     1861 aagacgggcc ttcaccgccc agtccggatc gctgagcagt gctcgaccta tgccaactag
     1921 gtcagctttg gattgccgta ttatggcatc ggccgtggcg ggtctcttga tgccgccggt
     1981 cacgatcaca ggcgctccgc agaccgcttt cacccggctg ctgatgtcga tgaagaagcc
     2041 ttcacccttc cactcgaagg gctgataccc tct
//
LOCUS       DAOTAC010000339         2045 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_41414_length_2045_cov_3.767839, whole genome shotgun sequence.
ACCESSION   DAOTAC010000339 DAOTAC010000000
VERSION     DAOTAC010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41414_length_2045_cov_3.767839"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..321
                     /gene="rpoZ"
                     /locus_tag="PLM74_11235"
                     /pseudo
     CDS             136..321
                     /gene="rpoZ"
                     /locus_tag="PLM74_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032952.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
     gene            451..1647
                     /gene="coaBC"
                     /locus_tag="PLM74_11240"
     CDS             451..1647
                     /gene="coaBC"
                     /locus_tag="PLM74_11240"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HON43682.1"
                     /translation="MDGVCVFGVTGGIAAAKATLVASMLAGQGLDVHVIMTESACEFV
                     TPLTFRTLTANPVTVDMFEEPGQWNVKHVSLAQRADLFVIAPATANFLGKTAAGIADD
                     MLTTTVMATRAPVVIAPAMNSNMYGNPIVQRNIASLKALGYMFVGPESGRLACGDEGA
                     GRMASPERIVDFAMHVLHGRGRPSLAGRRILVNAGPTREPIDPVRYITNRSSGKMGFA
                     IAGAAAARGAQVTLVSGPTNLPVPEGCALTPVETAEEMYQACMMAAPNADIMIAAAAV
                     ADYRPADYSRIKIKKTAGDMSIELERTRDIVAELGRTKGTRVIVGFAAETNDLLANAQ
                     AKLASKNLDIIVANTVGGEKSPFGSDDNTVTLLRPGLEPEALPAMSKLALAHEILDRA
                     ASIIRK"
     gene            1745..>2045
                     /locus_tag="PLM74_11245"
     CDS             1745..>2045
                     /locus_tag="PLM74_11245"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HON43683.1"
                     /translation="MSRPGHKRLGDSLVDSGAITLEQLDDALVVQRRTGERLGRALVK
                     LGYTTDATIAAVIERQFGVPRISLSEYVVASDMIALVPEAMARRYKVLPVEITGNK"
BASE COUNT          403 a          623 c          635 g          384 t
ORIGIN      
        1 ttcttttgta cgcaggttcg cgccggaatc acctagctag cgcaacctac acctcggcct
       61 ccctgacgcc gcgcgtgggc gccggcggat gaagggggct ttgtaatgga tgcacagtga
      121 atggaggcta cttccttgat tcgaccctca ttggaggaac tcatcccaat ggccgatagc
      181 cgttatacgc tggtagtgat ggctgccaag cgcgcgcgga ctatcatgaa tgtccagcag
      241 aagcggggcg gaagtctcgc tgaaaagccg gttacgcgcg ctctgcgcga gatcgccgac
      301 gggcagatcg acttctaccg tcctgctccg ggcgaggtag aggagactgg cgacggcttt
      361 cagatgccgg atgcccccat cgatcgcgat gagcctctgg cagacctctt cgcgcagctg
      421 ggcggcagct ccgacggcag gtgacgccgc ttggacggtg tatgcgtatt cggggtgacc
      481 gggggtatcg cagccgccaa ggccacactc gtcgccagca tgctggctgg gcaggggctg
      541 gacgtgcatg tgatcatgac cgagtcggcc tgcgagtttg ttacgcccct gaccttccga
      601 acgcttacag ccaatccagt cacggtggac atgttcgaag agccgggcca gtggaatgta
      661 aagcatgtgt ccctggccca gcgggctgat ctgttcgtaa tagctccagc caccgccaat
      721 ttccttggca agacagccgc cggcatagcg gacgacatgc tcaccaccac ggtcatggcc
      781 actcgcgctc ccgtcgtgat cgccccggca atgaacagca acatgtacgg taaccccata
      841 gtccagcgga acatcgcttc actcaaggcc cttggctata tgttcgtggg gcccgagtca
      901 ggccgcctgg cctgcggcga tgagggcgcg gggcgtatgg cctcacccga acggatcgtt
      961 gatttcgcca tgcacgtgct gcacggtcgg ggtcgtccgt cgcttgccgg caggcgcatc
     1021 ctggtgaacg cagggcctac ccgcgagccc atagatcctg tccgctacat taccaacagg
     1081 tcgtctggga agatgggctt cgccatcgcc ggcgcggctg cggccagagg cgcccaggtt
     1141 actctggtgt ccgggccgac gaacctgccg gtgccggaag ggtgcgccct cactcccgtg
     1201 gagacggccg aggagatgta ccaagcctgc atgatggcgg cgcccaacgc cgacatcatg
     1261 atcgccgccg ctgccgtcgc ggattaccgc ccggccgact acagccggat caagatcaag
     1321 aagaccgcgg gcgatatgag catcgagctg gagcgcaccc gcgatatcgt cgccgagctt
     1381 gggcggacca aaggaacgcg cgtcatcgtc ggctttgcgg ccgagacgaa cgacctgctc
     1441 gccaacgccc aggccaaact ggcgtctaag aacctagata tcatcgttgc caacacggtg
     1501 ggcggtgaaa agtccccgtt cggctctgac gacaatactg tcactctgct tcgcccaggc
     1561 cttgagcctg aggcgcttcc tgccatgtcc aaactggcat tggcccacga aatactcgac
     1621 agggccgcct ccatcatccg caagtagact gcagactcca agctattccg atgattcatg
     1681 catctttacc ggaaggattc ccgataggag cgtagaacat ccattcttgt ggagatgatg
     1741 aactttgtcg cgacctggcc acaagaggct tggagacagc ttggtcgact cgggagccat
     1801 aacacttgag cagctagatg acgctctggt agtgcagcgg cgcacaggtg agcggctcgg
     1861 acgtgccctc gtgaagctgg gctacactac cgatgctacg attgccgcgg tgatcgagcg
     1921 tcagttcggc gtaccccgca tcagcttgag cgagtatgtg gtcgctagcg atatgatagc
     1981 tctggtgccg gaggccatgg ccaggaggta caaggtcttg cctgtggaga tcacaggcaa
     2041 caagc
//
LOCUS       DAOTAC010000340         2040 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_41533_length_2040_cov_3.724433, whole genome shotgun sequence.
ACCESSION   DAOTAC010000340 DAOTAC010000000
VERSION     DAOTAC010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41533_length_2040_cov_3.724433"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="PLM74_11250"
     CDS             complement(<1..219)
                     /locus_tag="PLM74_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HON43684.1"
                     /translation="MKRLSCLLVLALVMSLSLGAHAAKVIEITYWTHTDDNRTAIENK
                     YIAEFEKMYPDVKVKRVVNEASKMGDLVL"
     gene            complement(414..1400)
                     /locus_tag="PLM74_11255"
     CDS             complement(414..1400)
                     /locus_tag="PLM74_11255"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HON43685.1"
                     /translation="MKPRIYVTRMLPQAGLDLLAEEYDVEVNPHDRVATREELLAGIR
                     GKDALLALLTDTVDAGVMDAEPRLKVIANYAVGFNNIDVAAATERGIPVTNTPGVLTE
                     TTADLAFTLLMACARRVVEADGFMRAGKYEGWGPMLFLGRDVHGKTLGLVGFGRIGQA
                     MAQRGLGFGMKILYHDAQRASEELEKAYRAEYRELPDLLRESDFVSLHVPLMESTHHL
                     ISDAQFELMKTTGILINTSRGPVVDEKALVRALKAGLISGAGLDVFEREPQFEPGLEK
                     LANVVMVPHIASASVETRTKMATMAAENACAAIRGEIPPNIVNPEVWDRRRR"
     gene            complement(1410..>2040)
                     /locus_tag="PLM74_11260"
     CDS             complement(1410..>2040)
                     /locus_tag="PLM74_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013626130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HON43686.1"
                     /translation="SIIVDMSSIAPGTSQEVAEEARKRGVRMLDAPVSGGQPGAVAAT
                     LSIMVGGDENDFDECYHMLKVMGSSVVRVGDIGAGNTVKLCNQIVVALNIAAVGEAFV
                     LGTKAGVDPNVIFAAIRGGLAGSTVMNAKVPLVVERKFDPGFRIDLHIKDLANALQTG
                     KALDVPLPFTGLVAQVMQALRVQGKGGLDHGAIMTFFEGLAGVEIRSKN"
BASE COUNT          411 a          654 c          523 g          452 t
ORIGIN      
        1 tagaacgaga tcgcccatct tgctggcttc gttcacgact ctcttgacct tgacatcagg
       61 gtacatcttc tcaaattcgg caatgtactt gttctcaatc gccgtcctgt tgtcgtcggt
      121 gtgagtccag taagtgattt caatgacttt cgctgcgtgt gccccaagac tgagcgacat
      181 aacgagggca agcaccagca ggcacgataa tctcttcacg taggtcccct cccagtcgat
      241 ttctcagtat actagctaac cctcccgatc cgcactggat tcgcgccgcg tcacctcccc
      301 gggaaggttc atggtagtcc atcattatga gaattcgtca atgcgaggaa gagtcctgcg
      361 cagctaacct ccaagtcatg acctgctgtg gtctccagat atcacaacgg gccttacctt
      421 ctccgcctat cccatacctc cgggttcacg atgttcggcg gtatctcgcc ccttatggca
      481 gcgcacgcat tctctgcggc catggtagcc atcttggttc tggtttccac cgacgcgctc
      541 gcgatatgcg ggaccatgac gacattggct agcttctcaa ggcccggttc gaactgaggt
      601 tcgcgctcga acacgtccag accagcgccg gatatgagcc cggccttcag cgccctgacc
      661 agagcctttt cgtcgactac aggccctcgg gatgtgttga tcaagatccc agttgttttc
      721 atgagctcaa attgcgcgtc gctgatcagg tggtgggtcg actccataag tggcacatgc
      781 aagctgacga agtccgattc cctgagcaga tcgggcagct ctctgtactc cgctctgtac
      841 gccttctcca gttcctccga agcgcgctgt gcatcgtggt acaggatctt catcccaaac
      901 cctagcccgc gttgggccat cgcctgaccg atgcgtccga agccaacaag gccaagagtc
      961 ttgccatgga catctctacc cagaaagagc atggggcccc aaccctcgta cttgcctgca
     1021 cgcatgaacc cgtcagcctc tacgacccta cgggcacacg ccatcagtag cgtaaacgcc
     1081 aaatccgcag tggtctctgt cagaacgccc ggagtgttag tgaccggtat gccgcgctca
     1141 gtcgcggcgg caacgtcgat attgttgaaa cccacagcgt agttggctat gaccttgagg
     1201 cgcggctcgg catccatgac cccggcgtct actgtatcgg taagcagagc cagcagggca
     1261 tccttgcccc ggattccggc gagaagctcc tcgcgcgtgg ccactctgtc atggggattg
     1321 acctcgacat cgtactcttc cgccagaaga tccagccccg cctggggcag catccttgtt
     1381 acgtatattc tcggtttcat ccacgcacct cagttcttgc tccgtatttc gactccggcc
     1441 aggccctcga agaaggtcat gatcgcaccg tggtccagac cgcccttacc ctgaacccgc
     1501 agagcctgca tgacctgggc cacaaggccg gtgaatggca ggggcacatc cagagccttt
     1561 cctgtctgca gcgcattggc caggtccttg atatgaaggt caatacggaa acccgggtcg
     1621 aacttgcgct cgacgaccag gggaaccttg gcgttcatga cagtgcttcc ggcaaggcct
     1681 ccgcgtatag cagcgaagat cacgtttgga tctaccccgg ccttggttcc aaggacaaaa
     1741 gcctctccca ctgcagcgat attcagggcg actacgattt gattgcacag cttcactgtg
     1801 ttcccggcac ctatgtcgcc taccctcaca actgaagagc ccatgacctt cagcatgtga
     1861 tagcactcgt cgaaatcgtt ttcatcgcct ccgaccatta tggagagggt agcggccacc
     1921 gcgccgggtt gcccgccgct cacaggcgca tcgagcatgc gcacgccacg cttgcgcgcc
     1981 tcctcggcca cttcctgcga ggtgcccggc gcgatcgaac tcatatccac gataatagag
//
LOCUS       DAOTAC010000341         2020 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_42068_length_2020_cov_3.586768, whole genome shotgun sequence.
ACCESSION   DAOTAC010000341 DAOTAC010000000
VERSION     DAOTAC010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42068_length_2020_cov_3.586768"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1373
                     /locus_tag="PLM74_11265"
     CDS             <1..1373
                     /locus_tag="PLM74_11265"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HON43687.1"
                     /translation="INVTRDNDITTGIIYSSVLSKERRGDYLGGTVQVIPHITNEIKD
                     RVTRLGQNGSDVVIVEVGGTVGDIESLPFLEALRQLRTEAGRENVMYIHVTLVPYISS
                     AGEFKTKPTQHSVKELRSIGLQPDVIVARSDRPLPADIKSKIALFCDVEPEAVIFNPD
                     AQSIYEVPLILQQEGLDEIVVKRLELACPAPEADMSAWRQMVTRLKSIKRSIRVALVG
                     KYVALPDAYLSAVEALRHAGIENGVQVEIDWIDSEALTSYAQAEQALSRAHGVLVPGG
                     FGPRGVEGKILAARYAREHKVPFLGLCLGMQCAVIEFARNVCGLRGANSTEFDAATAH
                     PVVDFMPGQRGIERIGGTMRLGVYPCALEPGSKAANAYGETLVHERHRHRHELNNAYA
                     DILAEHGLRFSGISPQRNLIEILELSDHPWFVGTQFHPEFKSRPGRPHPLFREFVAKS
                     LEYSKC"
     gene            1692..>2020
                     /locus_tag="PLM74_11270"
     CDS             1692..>2020
                     /locus_tag="PLM74_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HON43688.1"
                     /translation="MNIAELEKKTVSELHDIARDLGIPSHSQLRKQELTFEIVKAASQ
                     KEGNLWADGILEILPEGFGFLRVGGLTPSSDDIYISPSQIRRFVLRKGDMVSGQVRPP
                     KEGEKYFA"
BASE COUNT          435 a          566 c          600 g          419 t
ORIGIN      
        1 acatcaacgt cacccgcgac aatgacatca ccaccggcat catatactcc tcggtcctct
       61 ccaaagagag acgcggcgac tacctcggcg gaaccgttca agtcatcccc cacatcacca
      121 acgagatcaa agatcgcgtt actcgcctcg ggcaaaacgg atcggatgtt gtcattgtcg
      181 aggtaggcgg cacggtaggc gatatagaga gcttgccgtt tcttgaggct ctcaggcagc
      241 tcagaactga ggcgggccgc gaaaatgtga tgtatatcca cgtcacgctc gtgccgtata
      301 tctcctccgc gggtgagttc aagaccaagc ctacgcagca ctcagtcaag gagcttcgta
      361 gtataggact tcagcccgac gtgatcgtcg ccaggtccga ccgtccgctt ccagccgata
      421 tcaagagcaa gatcgctctg ttttgcgacg tggaaccgga agccgtgata ttcaaccccg
      481 atgctcaatc catatatgaa gtgcccctca tcctgcagca ggagggcctg gatgaaatcg
      541 tcgtaaagcg cttggagctg gcatgtcccg cacccgaggc ggacatgtcc gcgtggcggc
      601 agatggtaac caggctcaag agcatcaaac gctcaatccg ggtggcgctt gtgggcaagt
      661 atgttgcact gccggacgca tatctcagcg cagttgaggc gcttcgccac gcggggatcg
      721 aaaacggcgt gcaggtggag atagattgga tcgattccga ggccctgaca agttatgctc
      781 aggctgagca ggcgctttcc agggcgcacg gcgttctggt tcccggcgga ttcggcccgc
      841 gcggggtgga gggcaagata ctggcggctc gatatgcccg cgagcacaag gtgccattcc
      901 tcgggctatg cctggggatg cagtgtgctg tgatcgagtt cgcgcgcaat gtgtgcgggc
      961 tccgtggcgc caacagcact gaattcgatg ctgccaccgc ccatcctgtt gtcgacttca
     1021 tgccgggaca gcgtgggatc gagcgaatag gcggaaccat gcgtctcggc gtctacccct
     1081 gcgctctcga gccgggatcg aaggcggcta atgcctatgg tgagaccttg gtgcatgaga
     1141 ggcatcgcca ccgccatgag ctcaataacg cgtatgccga catccttgcc gaacacggtc
     1201 tgaggttctc agggataagc ccccagcgca acctcattga aattctggag ctttccgatc
     1261 acccatggtt cgtggggacg caattccacc ccgagttcaa gtccaggccg ggcagacctc
     1321 atcctctgtt ccgggagttc gtcgcaaaat cgctcgaata ctcgaaatgt tgatagccgc
     1381 cctgtgctgg ggatatgaaa cccgttccgc tgggcacaat tgattgtgga atatgcgccg
     1441 gaggattttg gcgtaacgcg gagaatacgg tctgtaagca tgccaggctg agtgcgtggc
     1501 cgcactattt cctcaagcac gcctcatcgg ttgagccata agcgtaccat tcgtatagct
     1561 ccacaggagt tccctattgt ttacggctgg gggattgccc ctcgtaggcg gtccagccgc
     1621 ccggaatatg ctgcctgccc gctgatttgt actctattgt ccatataccc tgaaaggaag
     1681 atgcgctaca gatgaatatc gcggagctag agaagaagac cgtgtccgag ttgcatgaca
     1741 tcgctaggga cttgggcata cccagtcact cgcagttgcg caaacaggag ctgacatttg
     1801 agatcgtcaa agcggcgtct cagaaagagg ggaatctgtg ggccgacggg attctggaga
     1861 tcctccccga gggtttcggg ttcctgcgtg tgggagggtt gactcccagt tcagacgata
     1921 tctacatctc cccctcacag atacgcaggt tcgtgctgcg caaaggagac atggtctcgg
     1981 ggcaggtgag gccgcccaag gagggcgaga agtacttcgc
//
LOCUS       DAOTAC010000342         1925 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_44770_length_1925_cov_3.459893, whole genome shotgun sequence.
ACCESSION   DAOTAC010000342 DAOTAC010000000
VERSION     DAOTAC010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44770_length_1925_cov_3.459893"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="PLM74_11275"
     CDS             <1..642
                     /locus_tag="PLM74_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HON43689.1"
                     /translation="KTFGAVASLYPEVIQFVVRADGPIKTWADVRGKRISVGAPGSGT
                     EANARQILDIYGMTYADVEEQFLSFAESANQFKDGHIDGFFVTAGVPNPGITDVSTQH
                     KIKILSMPADKMKQLTTKYPFLVAATVPANSYLNQTEAASTPAVMAILIAGNAVPEAD
                     VYQITKILFENDGDLAKAHAKGAEIKLATALNGLSTPLHAGAAKYYREKGILK"
     gene            787..1308
                     /locus_tag="PLM74_11280"
     CDS             787..1308
                     /locus_tag="PLM74_11280"
                     /inference="COORDINATES: protein motif:HMM:NF020479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1850 domain-containing protein"
                     /protein_id="HON43690.1"
                     /translation="MRPNRFRLTMPIPLVVALAVVSVIVLVMALVRPHAMVLQAVASG
                     GRLLYEHQVAPGDEFEISYTHSVARTVVLEKFRVTPDRQILLYETVYQDFGAGLPSEL
                     DEGAQVEVGPGGVRITGMKRVMSSIPLRVGSIARHQISCGSDVVDLISLVESGERVDI
                     AVAAKAMLPYTRR"
     gene            1317..>1925
                     /locus_tag="PLM74_11285"
     CDS             1317..>1925
                     /locus_tag="PLM74_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43691.1"
                     /translation="MGTNDDDRKRLPETEDAVDIDEILAKYDRESATRHLAGWLTWAV
                     RIIALAFAIFQLYTAIRGERPPQIQRVTHLGFVLTLTYLLYPPTTKGRDKFSWFDLLL
                     AIAGAGVAAYYLTNYQHLMTRAGDYTTMDMFVGAIAILLVLEAARRVVGLPIVVILCV
                     CLLYAYFGKRMPGFLRHRGASFERLTTHMFFTTEGILGIPLGV"
BASE COUNT          417 a          561 c          562 g          385 t
ORIGIN      
        1 aagaccttcg gcgccgtggc gtcgctgtat cctgaggtca tccaattcgt ggtacgcgca
       61 gatggcccca tcaagacctg ggccgacgtc cggggcaaga gaatctccgt gggcgccccc
      121 ggcagcggca cagaggcgaa cgcacgccag attctcgaca tatacggcat gacctatgca
      181 gatgtggagg agcagttcct ctcatttgct gagagcgcca accagttcaa ggatggccac
      241 atcgatgggt tcttcgtcac agccggcgtt cccaacccgg ggataaccga cgtgtcgacc
      301 cagcataaga tcaagatcct gtcgatgccg gccgacaaga tgaagcagct taccaccaag
      361 tatccgttcc tagtcgcagc cacggttccg gccaactcct atctgaacca gaccgaagcg
      421 gcgagcactc ccgcggtcat ggcgatactc atagcgggca acgctgtgcc tgaggccgat
      481 gtgtaccaga taaccaagat cctcttcgaa aacgacggcg acctcgcgaa agcccatgcc
      541 aagggagccg agatcaagct ggccactgct ctcaacgggc tttcgacgcc tctgcacgca
      601 ggggcagcca agtactatcg ggaaaaggga atcctcaagt aaggcccatt agtctgtttg
      661 acaaccggcg cgctgagcgt cgcaggtcta cggtgagcac ccagcgtgcc tgacgaggtg
      721 aacaggaagc gcggcagcgt atgagagaag cttggagcgc tgccgcgcat tcattcagaa
      781 atgagcatga ggccaaaccg tttccgactg accatgccga ttccccttgt agtcgcgttg
      841 gcagtagttt ccgtgatagt gctcgtgatg gcgctcgtgc gcccacacgc catggtgctt
      901 caagcagtag ccagcggagg gcggttactg tacgaacacc aggtggcgcc gggcgatgag
      961 tttgagatca gttatacgca ctcagtagcc aggaccgttg ttctggagaa gtttcgcgtc
     1021 acacctgata ggcagattct gctgtatgaa acggtctatc aggactttgg cgcaggtctg
     1081 ccgagcgaac tggacgaagg agcgcaggtg gaagtgggcc ccggcggagt gcgaatcacc
     1141 gggatgaaga gagtcatgag ctcaattccg cttcgcgtcg gcagtattgc acggcatcag
     1201 atctcttgtg gcagcgatgt cgtcgacctc atatccctgg tcgaatctgg tgagcgcgtc
     1261 gacattgccg ttgccgcgaa agccatgttg ccgtacacga ggaggtagta ctcgctttgg
     1321 gcaccaatga tgacgaccgc aagagattac ccgaaaccga agacgcagta gatatagacg
     1381 agatactcgc gaaatacgac cgcgaatcgg ccactcggca tctggctggc tggttgacat
     1441 gggccgtaag gataatcgcc ctagcattcg ccatcttcca gctttacacc gcgatccggg
     1501 gagagcgtcc gccccagatt cagcgcgtca ctcacctagg attcgtcttg acgctgacct
     1561 acctgctgta tcccccaacc acgaaggggc gcgataagtt ctcatggttc gatctgctgc
     1621 ttgcgatagc cggcgcaggc gtggcggcct attatctcac caactaccag catctgatga
     1681 cacgcgccgg cgactacacc acgatggaca tgttcgtcgg agctatcgcc atcctgctcg
     1741 tgctggaggc tgcacgccgg gtggtaggtc tgcccatcgt ggtgatactc tgtgtgtgcc
     1801 tgctctatgc ctacttcggc aaacggatgc cgggcttttt gaggcaccgc ggggcatcct
     1861 ttgaacgcct caccacccac atgttcttca ccactgaggg catactcggc ataccgcttg
     1921 gtgtg
//
LOCUS       DAOTAC010000343         1892 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_45766_length_1892_cov_6.253130, whole genome shotgun sequence.
ACCESSION   DAOTAC010000343 DAOTAC010000000
VERSION     DAOTAC010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45766_length_1892_cov_6.253130"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="PLM74_11290"
     CDS             <1..191
                     /locus_tag="PLM74_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HON43692.1"
                     /translation="AASSHFAVRKEERGGRTQVGIWHLDRDERVAEVARMLGGDSPSA
                     LTVAHAQEMLEKLDGGRI"
     gene            282..593
                     /locus_tag="PLM74_11295"
     CDS             282..593
                     /locus_tag="PLM74_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008713130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HON43693.1"
                     /translation="MRLKDHPILEFPTGREVVFTFNGKEIQGIEGEPIAAALHAAGVR
                     VLRHSPKLGRARGFFCAIGTCSSCLMTVDGVPNVRVCTEPLKQGMVVETQKGHGHLPI
                     D"
     gene            623..1717
                     /locus_tag="PLM74_11300"
     CDS             623..1717
                     /locus_tag="PLM74_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007956730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HON43694.1"
                     /translation="MREVDIAVIGAGPAGLAAALACGRSGATTLLVDYMDAPGGQLIK
                     QTHKFFGSERQFAGTRGIQIPDIFINQLSKMPNVALMWGANASGYYADSGVLTLEDGG
                     KFIKVKAKRVIIATGAAEKALAFENNDLPGVYGAGAVQTLMNVHGVKPGDRVLMVGSG
                     NIGLIVSYQLIQAHVEVVALVEAAGCIGGYLVHAAKLRRAGVPILTSHTIVRALGEQT
                     VRGAVVAQVDHSWQVKPGSEREVACDVICLAVGLTPLVDMMWQAGCEMRYVPALGGHV
                     PVRDENMRASNPAVYVAGDAGGVEEASSAMISGEIAGISAAKSLGFEVEGADARLSQL
                     KADLAAIRSGPTGEKICAGIERAMVCGGEW"
     gene            1717..>1892
                     /locus_tag="PLM74_11305"
     CDS             1717..>1892
                     /locus_tag="PLM74_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014826221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HON43695.1"
                     /translation="MAERETQDDNIVICRCEDITLSEIRDLIAQGYHTIDEIKRVSRA
                     GMGPCQGRTCRQLIA"
BASE COUNT          387 a          479 c          661 g          365 t
ORIGIN      
        1 ctgctgcatc tagccacttc gctgtgcgca aagaggagcg gggaggcaga acccaggtgg
       61 gcatctggca tctggaccgg gacgagcgcg tggctgaggt agcaagaatg ctgggtggcg
      121 acagcccctc cgcgcttacg gtggcgcacg cgcaggagat gctagaaaag ctcgatggag
      181 gcaggatttg agtctctttt gtggaaaatc cgctatatag ccatttcgct caggggaggg
      241 aggactcggg ccgcccgctt gggcggctac ggccgaatgt ggtgaggtta aaggaccacc
      301 ctattctgga attcccgacg ggacgggagg tagtgttcac tttcaacggc aaggagatcc
      361 aaggtatcga gggagagccc attgcggcag ctctgcacgc tgccggtgta agagtcctcc
      421 gtcacagtcc gaaactcggg cgtgcgcggg ggttcttttg tgccatcggc acctgctcat
      481 cctgcctgat gactgtggac ggtgttccca atgtacgcgt atgcactgag cctttgaagc
      541 aagggatggt agtcgagact cagaaaggcc acggccactt gcccattgac tgaggcgggg
      601 ctgagtaagg acggtgacgc aattgagaga agttgacatt gccgttatcg gcgccgggcc
      661 tgcagggtta gcagccgccc tggcgtgcgg cagatcggga gccacgactc ttctggttga
      721 ctacatggat gcccccggag gccaactgat caagcagact cacaagttct tcgggtcgga
      781 gcggcagttc gcgggcactc gcgggattca gatccccgac atcttcatta accagctttc
      841 taagatgccg aacgtagcac tgatgtgggg cgcaaatgcg tctggctact atgcggattc
      901 cggagtgctt acgctggagg atggcggcaa gttcatcaag gtgaaggcaa aacgagtgat
      961 tattgcgaca ggcgctgccg aaaaggcgct ggcgttcgaa aacaacgatc tgccgggcgt
     1021 gtatggggcg ggcgcggtac agaccctcat gaacgtgcat ggcgtgaaac ccggcgaccg
     1081 cgtgctcatg gtgggctcgg ggaacattgg attgatcgta agctaccagc tcattcaggc
     1141 gcacgttgag gtggtcgcat tggtggaagc cgccgggtgc ataggtggat atctggtgca
     1201 tgcggccaag cttcgcaggg ccggggtgcc gattctgaca tcgcacacca tcgtccgggc
     1261 gctcggcgaa cagacggtgc gaggcgcggt agtcgcccag gtagaccatt cctggcaggt
     1321 aaagccggga tccgagcgcg aagtagcgtg tgatgtgata tgccttgcag tggggcttac
     1381 tcctctggtg gacatgatgt ggcaggctgg gtgcgagatg aggtacgtgc cggctctggg
     1441 tgggcatgtt ccggttaggg atgagaacat gcgagcctcg aaccctgccg tgtatgtggc
     1501 gggcgatgca ggcggagtgg aagaggccag ctcggccatg atctccggcg agatcgccgg
     1561 aatatctgcg gccaagtcgc tcgggttcga agtcgaaggg gccgatgcgc gtctttcgca
     1621 gcttaaagcg gatctggctg cgataaggtc gggccccacc ggtgagaaga tctgcgcagg
     1681 catcgagcgt gccatggtgt gcggaggtga gtggtgatgg ccgaacgaga gacccaggac
     1741 gacaacatcg ttatctgcag gtgcgaagac atcacgcttt ccgaaatcag ggacctgatc
     1801 gcgcagggct atcacaccat cgacgagatc aagagggtgt cgagggccgg tatggggccg
     1861 tgccagggca gaacgtgccg tcagctgata gc
//
LOCUS       DAOTAC010000344         1823 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_48019_length_1823_cov_3.068439, whole genome shotgun sequence.
ACCESSION   DAOTAC010000344 DAOTAC010000000
VERSION     DAOTAC010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48019_length_1823_cov_3.068439"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="PLM74_11310"
     CDS             <1..490
                     /locus_tag="PLM74_11310"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HON43696.1"
                     /translation="VTGTIEFPVGTAYILTDKYSTYGDISGSVKIAIPGSLAVDNKFF
                     KVGSSIFTAEYGSSDSSVVSVDGNGRMTVRNEGRADITVAIGSARILIPVTVTRLPIG
                     LYSSKSDIVQAMGFPDDITPVGYGTELWRWNKHSDLCLAVGGVRVEVRSRWSGRFDSI
                     WK"
     gene            554..1066
                     /locus_tag="PLM74_11315"
     CDS             554..1066
                     /locus_tag="PLM74_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43697.1"
                     /translation="MKSCPDCGTENSELNGFCSKCSAALPRKLNISSAPITPHVEKRQ
                     ASDSEASETERLIKRTEGRLSDISSQMVSHRCGLAGALSALLPGLGQIYAGDIIKGMA
                     MVASWVVGGFFYGRWIIASIGARSELYPWANSWDRSDLGLGHLLVGLVMIGFWVYAIV
                     DAASAPERLR"
     gene            1177..1302
                     /locus_tag="PLM74_11320"
     CDS             1177..1302
                     /locus_tag="PLM74_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43698.1"
                     /translation="MPPYNRFERAALQAIQQAVDEAADGVASFTWDELAGKASLM"
     gene            complement(1618..>1823)
                     /locus_tag="PLM74_11325"
     CDS             complement(1618..>1823)
                     /locus_tag="PLM74_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43699.1"
                     /translation="AGASATVAGSSLQEKCPMGYGGGTAFPEFLEAPTRADTAPLTGG
                     RLAASGTEGGQLLTPEGPAHDRG"
BASE COUNT          345 a          526 c          549 g          403 t
ORIGIN      
        1 cgtcacgggt acgatcgagt ttccggttgg gacagcctac atactgactg acaagtactc
       61 gacctatggc gacatcagtg gttctgtaaa gatagcaata cccgggtcct tggccgtcga
      121 caacaagttc ttcaaggtcg gaagctctat cttcactgct gagtatggct cttcggactc
      181 gagcgtagta tcagtggatg ggaatggtcg catgactgta cgcaacgaag ggcgagcaga
      241 tatcactgtt gcaataggca gcgcccgcat tctcattccg gtcacggtga ctaggctgcc
      301 gatagggctg tactcatcga agtccgacat tgtgcaggct atgggtttcc cagacgacat
      361 tacgccagtt ggttatggca ccgaactgtg gagatggaac aagcattcgg atctatgctt
      421 ggctgttggt ggcgtgcgcg tagaagtccg ctcgaggtgg agtggcagat tcgatagtat
      481 ctggaagtag aggttgcctg cgctgccgga ggactgcccc tgccggtcgg gggctctgct
      541 gaggtggtgt ctgttgaagt cctgccccga ctgcggcaca gagaacagtg agctcaatgg
      601 attctgctcc aagtgctcgg ccgcgctgcc acgtaagcta aacatctcat ccgccccgat
      661 tacgcctcat gtggagaaac gccaggcatc cgattccgag gccagcgaga ccgagagact
      721 aatcaagcgg actgagggtc gcctatccga cattagcagc cagatggttt cgcaccgctg
      781 cgggttggcc ggcgcgctct ccgccctgct gcccggattg gggcagatat acgctggaga
      841 tatcatcaag ggcatggcca tggttgccag ctgggtggtc ggcgggtttt tctatggacg
      901 ctggattatc gcgtcgatcg gcgccagatc cgagctgtac ccgtgggcaa acagttggga
      961 cagatctgat cttggtcttg ggcacctgct tgtcgggctt gtcatgatcg ggttctgggt
     1021 ctacgccata gtcgatgccg caagcgcgcc ggaacgtctg cggtgatgag cgtctccctc
     1081 cgctccctcg cctgggcctt ctcgatatgc cagcacatga tggagctgcg gggcaggcaa
     1141 cgtgacgcat ctgcagcata gatcggaggt gacgccgtgc caccgtacaa ccgctttgaa
     1201 cgagctgcct tgcaggcgat acagcaggcc gtagacgagg ccgccgatgg agtcgcgtct
     1261 ttcacgtggg acgagctagc gggaaaggcg tccttgatgt aacgccgcaa tatttccagg
     1321 ttatcgccat agtagagtgc gttcgcgtgt tcctcagcca tcgccaaacc ctctcctaac
     1381 gaaccgtcat cttccttgcc ttctttcgcc aatcgttgct tttcgccttt tctcgaagtc
     1441 aaacgccttg cgcccgtgct gtcagttcgt ctggagccgc tgaaaccttg ctgccagcgc
     1501 gagtagtact gtgagtgcgg aatgcggcgc ttaactggca ccatgggtac tagtcgccca
     1561 gtcagaacat cgcggagcat cacgggccga atcaccgcaa catttcgcaa gtttgcgtta
     1621 cccccggtca tgtgcgggcc cttccggggt gagcagttgc ccgccttcgg tcccgcttgc
     1681 cgccagccgc ccgcccgtca ggggggccgt gtccgcgcgc gtaggagcct ctagaaactc
     1741 gggaaacgct gtaccccctc cataccccat ggggcacttt tcttgcaatg agctgcccgc
     1801 cacggtcgca gaagcgcccg cct
//
LOCUS       DAOTAC010000345         1778 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_49637_length_1778_cov_3.882763, whole genome shotgun sequence.
ACCESSION   DAOTAC010000345 DAOTAC010000000
VERSION     DAOTAC010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49637_length_1778_cov_3.882763"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="PLM74_11330"
     CDS             <1..277
                     /locus_tag="PLM74_11330"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HON43700.1"
                     /translation="NMESWTQLLCSADDAILVDNVLGMHTQDPTNGNFSLAVPDGIVV
                     RAGQFAGAAKVVISGNFFKILNQVDTRFAMSEDDPNPGIELTCHVES"
     gene            340..1161
                     /gene="mtnP"
                     /locus_tag="PLM74_11335"
     CDS             340..1161
                     /gene="mtnP"
                     /locus_tag="PLM74_11335"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HON43701.1"
                     /translation="MRLAIIGGTGVYDPAILDNVREATVQTPYGPASMKIGSYRGQEI
                     AFVARHGFGHTVPPHMINYKANIAALKILGVERVIATSAVGSLNPEMKPGMFVFVDQF
                     IDFAKSQVRTFFDGGEHGVVHLDYTEPYCPTIRAKMEEGARALGIEAINGGCYVCLDG
                     PRFETPAEIRMFAKLGGDLVGMTGVPEVVLAREAQLCYASIAMVTNWASGISDTALTH
                     QEVVDIMQANGEKLRAVIMSAIDNLPLERGCACAEAGVEMPWLAEYKEGGSRWQR"
     gene            1149..>1778
                     /locus_tag="PLM74_11340"
     CDS             1149..>1778
                     /locus_tag="PLM74_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HON43702.1"
                     /translation="MAAVGDLIIDDVTVITMDSERRMMRNAAVVVESGAIVYVGPATG
                     LLEASDGGPWAGLTKRLERMPRLQGRGRIAIPGLVNAHTHAAMVLLRGYADDLMLQEW
                     LTTKIFPIEARLTPDDVYWGSLAACAEMIKSGTTAFADMYFFMDEAARAVEKSGMRAS
                     LSVGMGSLAVSAEDPDGPDSLDSSGLGGFGAVLERGAALCERWNGAAGGR"
BASE COUNT          366 a          457 c          603 g          352 t
ORIGIN      
        1 gaacatggag agctggacgc agcttctttg cagcgccgac gatgcgatac tcgtcgacaa
       61 cgtgctgggc atgcacaccc aggatccgac taacggcaac ttttcgctgg ctgttccaga
      121 cggtattgtc gtgcgcgcgg ggcagttcgc gggggcagcc aaggtggtca tatcaggtaa
      181 tttcttcaag atcctgaacc aggtcgatac caggtttgct atgagcgagg atgatcccaa
      241 ccccggtatc gagctcacat gtcatgtgga gagctgacgg tggagatcag cgtgctgtca
      301 gtgcacggag cttgtgggaa atagcggagg tggaagtgta tgaggcttgc gataatcggt
      361 gggacaggtg tatatgaccc ggctattctg gacaatgtca gggaggcgac tgtgcagacg
      421 ccttacgggc cggcgagcat gaagatcggg tcataccgtg ggcaggagat tgcgtttgtg
      481 gccaggcacg gattcgggca cactgtgccc ccgcacatga tcaactacaa ggccaacatt
      541 gcggctctca agatcctggg tgtagaacgt gtcatagcta catccgcagt gggttcactt
      601 aaccccgaga tgaagccggg catgttcgtc ttcgtggacc agttcataga tttcgccaag
      661 tcacaggtga gaacattctt tgacggtgga gagcatggag tcgtacacct cgattacacc
      721 gagccgtact gccccacgat ccgcgccaag atggaggagg gcgcgcgcgc ccttggaatc
      781 gaagcgatta atggcgggtg ttacgtgtgc ctagatgggc cgcggttcga gacgccagcc
      841 gagatccgta tgttcgcgaa actcggaggc gaccttgtgg gcatgacggg cgttcccgag
      901 gtggtcctag cgcgagaggc tcagctatgc tacgcttcca tagccatggt tacgaactgg
      961 gcttccggca tctcagacac cgcccttacg caccaggaag tggtagacat aatgcaggca
     1021 aacggagaga aactgcgcgc cgtaatcatg tcggccatcg ataacctgcc ccttgagcgc
     1081 ggatgcgcct gcgccgaggc cggcgtggaa atgccgtggc ttgcggagta caaagaaggg
     1141 ggatcgcgat ggcagcggta ggcgacctga tcatagatga tgtcactgtg atcacgatgg
     1201 actccgagcg tcgcatgatg cgcaatgcag ccgtcgttgt cgagtcaggc gccattgtct
     1261 acgtagggcc tgcgactggg ctgcttgaag cgtcggacgg ggggccatgg gctgggctca
     1321 cgaagcggct tgagcggatg ccgcgtcttc agggccgggg ccggatcgcc attccaggct
     1381 tggtgaacgc gcacacgcat gccgccatgg tgctgcttag gggatatgcc gacgacctga
     1441 tgctccagga gtggcttact accaagatct ttcctatcga ggccaggcta actccggatg
     1501 acgtgtactg ggggagtctg gcagcctgcg cagagatgat caaatcaggc accacggcct
     1561 ttgccgacat gtatttcttc atggacgaag ccgcgcgggc ggtggagaaa tcgggaatga
     1621 gggctagcct atcggtggga atggggagcc tcgcggttag tgcggaggat ccggacggcc
     1681 ccgacagctt ggacagctcg ggcttgggcg gcttcggggc ggtgcttgag cgcggagccg
     1741 ctttgtgcga aagatggaat ggcgcagcgg gcggcagg
//
LOCUS       DAOTAC010000346         1766 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_50048_length_1766_cov_4.765050, whole genome shotgun sequence.
ACCESSION   DAOTAC010000346 DAOTAC010000000
VERSION     DAOTAC010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50048_length_1766_cov_4.765050"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..>1766)
                     /locus_tag="PLM74_11345"
     CDS             complement(30..>1766)
                     /locus_tag="PLM74_11345"
                     /inference="COORDINATES: protein motif:HMM:NF015644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HON43703.1"
                     /translation="LVEEKEYAVTEGITAAKLEGAEDTLANVRLWDWDPLLSTFEQLQ
                     EMRLYYHFADIDIGRYTIDGKTTQVMLSAREMDVSRLPSQAQTWVNTRLKYTHGYGAC
                     ASPVNEIGQDGLPTFLVADIPPRSPADMRLDRPEIYFGELTGDWVIVNTRAEEFDYPV
                     GDSNEYSRYDADSGIRMGYLFRRLLFATRFNDSKIIFSRDITPDSRMLFARDIRERVR
                     RIAPFLQYDGDPYAFIADGRLMWLIDGMTTSTAYPYAERFGDHLNYIRNSVKVSIDAY
                     TGEVNFYLIDPDDPIAVTYAKVFPGLFRPASEMPESIRQHLRYPEDLFKIQTRMYAMY
                     HMTDPEVFYNKEDLWGLPREIYKGQQQEVSPYYVNMQLPGESGVSFVLFTPFTPSRKD
                     NMVAWLAVSSDADEYGRMVAVNFGKQRVVFGPMQIEAQIDQDAEISKMLSLWNQSGST
                     VIRGNLLVYPINGSILYVEPLFLAAQASQLPQLKRVIVSDGTRVEIGSDLPEALEALV
                     GRRRTTGASAEIGMPGQAGQSVDRQVSQSAGAALDLYRKAQERLREGDLAGYDKYQRQ
                     LEQVLKSMAGEE"
BASE COUNT          356 a          536 c          478 g          396 t
ORIGIN      
        1 cgatggtcga cacactccat catgcgctgt tattcctcgc ctgccataga cttgagcacc
       61 tgctcaagct gcctctggta tttatcatag cccgccagat cgccctcgcg cagtcgctcc
      121 tgcgccttcc ggtagagatc gagagccgcg cccgcggatt gactgacctg cctatcaact
      181 gactgacccg cctggccagg catgccgatc tccgcagacg cacccgtagt cctcctccgc
      241 ccgaccagcg cctccagcgc ttcaggcagg tcagatccga tctccactcg ggtgccgtcg
      301 gacacgatca cccgcttcag ctggggtaat tggcttgcct gcgccgccaa gaacagcggt
      361 tccacgtaga gtatgctgcc atttatcggg tagacgagaa ggttgccgcg gataacagta
      421 gatccgctct gattccacag cgaaagcatc ttggatatct cggcatcctg gtcgatctgg
      481 gcctcaattt gcatcggccc aaacaccact cgctgcttgc cgaagttaac cgcgaccatg
      541 cgcccatact cgtccgcatc ggagctgaca gcaagccagg cgaccatgtt gtctttgcgc
      601 gaaggcgtga acggggtaaa cagcacgaag ctgactcccg attcgcccgg caactgcatg
      661 ttcacgtagt acgggcttac ctcttgctgc tgacctttgt agatctcccg gggtaggccc
      721 cataggtctt ctttgttgta aaacacctcg ggatcggtca tgtggtacat tgcatacatc
      781 cgagtctgta tcttgaacag gtcttcagga taccgcaggt gctgcctgat cgactcgggc
      841 atctcgcttg ccggcctgaa cagccccggg aagaccttag cgtatgttac ggctatcggg
      901 tcatcggggt cgatgaggta gaagttgact tcccctgtat aggcatcgat cgacaccttc
      961 accgagtttc tgatgtagtt gaggtgatct ccgaaacgtt cagcataagg gtacgcagtg
     1021 gacgtggtca tcccatctat cagccacatc agccgaccgt cggcgatgaa ggcgtatgga
     1081 tctccgtcat actgcaagaa cggagcaatt ctgcgcacac gttcgcggat gtcgcgcgca
     1141 aagagcattc tactatcggg cgtgatgtcg cgcgagaaga ttatcttgga atcattgaac
     1201 cgcgtcgcaa acagcaacct ccggaagagg taccccatgc gtatgccgga gtccgcgtcg
     1261 tatcttgagt actcgttaga gtcgcccaca gggtagtcga actcctcagc cctggtattc
     1321 actatgaccc agtccccggt cagctcgccg aagtatatct cggggcggtc aagacgcatg
     1381 tcggccggag accgcggcgg aatatcggcc accaggaacg tgggaagccc atcttggcca
     1441 atctcgttga ccgggcttgc gcaggcgccg tagccgtggg tgtacttcaa ccgtgtgttc
     1501 acccatgtct gggcctgcga tggcaggcgg ctcacatcca tctctctggc tgatagcatg
     1561 acctgcgtcg tcttgccatc aatggtgtac ctgccaatgt cgatatctgc gaaatggtag
     1621 tacagacgca tctcctgaag ctgttcaaaa gtggagagga ggggatccca gtcccatagc
     1681 ctcacgttgg ccaacgtgtc ttcagccccc tcgagcttgg ccgcggtgat gccttcagtc
     1741 actgcgtatt ccttctcttc caccag
//
LOCUS       DAOTAC010000347         1764 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_50125_length_1764_cov_4.866004, whole genome shotgun sequence.
ACCESSION   DAOTAC010000347 DAOTAC010000000
VERSION     DAOTAC010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50125_length_1764_cov_4.866004"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="PLM74_11350"
     CDS             complement(<1..532)
                     /locus_tag="PLM74_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON43704.1"
                     /translation="MNASDSGSSQTRNSSPTISPGPVARVAMVLVGLFVLGASIALYV
                     RADVGADPFTVLALGISNRTGATVGVATQLVGAGLLILLLVADRSHIGIGTLLNAVLV
                     GGSADIVLRFAPRPVSLLSTVAMTAAAILMMGVGVGLYVSGGLGEGALEGVMLVLSDC
                     LRIPVRLSRICMDVAAV"
     gene            complement(529..1704)
                     /locus_tag="PLM74_11355"
     CDS             complement(529..1704)
                     /locus_tag="PLM74_11355"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006570123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HON43705.1"
                     /translation="MQVHLARPDITQAEIDAVSEVIQSPILALGPWAMRFEKAIADYV
                     GAKHAIAVNSGTSGLHLLVRAFEIGEGDEVITTPFSFIASSNCIVYERARPVFVDIEL
                     STANIDSSLIEDAITPRTKAILPVDAFGRPARLDNIREIASRHGLRVIEDSCESLGSE
                     YKGTRTGNGAFCDGAVFAFYPNKQITTGEGGMVVTNDDRTAFLARSMCNQGRGETGVW
                     LSHERLGYNYRMDELSAALGAVQMSRIDEIIAKRQRVADMYNERLPKIPGVELPQVDP
                     DVTRMSWFVYVIRVGVDEQSHDRQSAVRDHVMQRFQEANIGCRPYFTPIHLQPYYRDA
                     FGFKEGDFPMTELAGRTSIAIPFHNHITCDEVEYVAQTLERALGETRGSKFFAERGI"
BASE COUNT          356 a          558 c          497 g          353 t
ORIGIN      
        1 ggactgctgc aacatccatg catatgcggc tgagccggac cggaatccga aggcaatcac
       61 ttagcaccag cataacgcct tccagggccc cttctcccag cccaccgctg acgtacaggc
      121 ccacgcccac gcccatcatg agtatggctg ccgccgtcat ggccaccgtg gacagaaggc
      181 tcacaggccg gggagcaaac cgcagcacga tgtcggcaga cccgcccaca agcaccgcgt
      241 tgagcaacgt accgatgcca atatggcttc ggtctgcaac aaggagaagt atgaggagcc
      301 cggcgcccac cagctgggtg gccacgccta cggtagcgcc ggttcggttt gaaatgccca
      361 gagccaacac ggtgaaagga tcggctccta cgtctgcccg aacatataac gcaatgctcg
      421 caccgagcac aaatagcccg accaacacca tcgcgacccg agctaccggg cccggcgaga
      481 ttgtcgggct cgagttccgt gtctggctcg agccggagtc tgaagcgttc atatgccgcg
      541 ctccgcgaag aacttgcttc ccctggtctc acccagggcg cgttcgagtg tttgggccac
      601 atactccact tcatcgcagg taatgtgatt gtggaacggt atggctatac tggtccgccc
      661 ggccagttca gtcatgggga aatcgccttc cttgaagccg aacgcgtcgc ggtagtaagg
      721 ctgcaggtga atgggggtga agtaaggacg gcaccctatg ttcgcctcct ggaatcgctg
      781 cattacgtga tcgcgtactg ccgactgtcg gtcgtgtgac tgctcgtcaa cgccgacgcg
      841 tatgacgtac acaaaccagc tcatcctggt gacgtccgga tctacctggg gcaattcgac
      901 acccggtatc ttgggcaagc gttcgttata catgtcggcc acacgctggc gcttggcgat
      961 aatctcgtcg atcctggaca tctgcacagc gcccagagca gccgatagct cgtccattcg
     1021 gtagttgtag cccaggcgct catgggatag ccacaccccg gtctctccgc gcccctgatt
     1081 gcacatactc ctcgctagga aggccgtgcg gtcatcgttc gtaaccacca tgcccccctc
     1141 gcccgtagtg atctgtttgt tcgggtaaaa cgcaaagacc gcgccatcac agaaggcgcc
     1201 gttgccggtg cgcgttccct tgtattctga gcccagcgac tcgcaggagt cctcaatgac
     1261 tctgaggccg tgccttgagg cgatctcccg aatattgtcg agcctggcgg gccgaccaaa
     1321 tgcgtccacc ggcaagatgg ccttagtgcg gggagttatc gcgtcttcta tgagactcga
     1381 atctatgttc gccgtgctaa gctcaatgtc cacaaatacg ggccgggccc gctcatacac
     1441 aatgcagttg ctggacgcaa tgaagctgaa tggggttgtt atgacctcgt cgccctcgcc
     1501 gatttcgaag gcgcgcacca gcagatgcag cccgcttgtc ccgctgttta cggcaatcgc
     1561 gtgctttgcc ccgacgtagt cggcgatcgc cttctcaaac cgcatggccc aggggcctag
     1621 ggccaatatg ggtgattgga taacctcact aactgcgtcg atttcggcct gcgtgatatc
     1681 cggacgcgcg agatgaacct gcaacttgcg gccctcctta ggtagtccac ttctgaaacc
     1741 gcccggtgcc cgccaagcgg gttc
//
LOCUS       DAOTAC010000348         1681 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_53352_length_1681_cov_5.197417, whole genome shotgun sequence.
ACCESSION   DAOTAC010000348 DAOTAC010000000
VERSION     DAOTAC010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53352_length_1681_cov_5.197417"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /gene="rpsO"
                     /locus_tag="PLM74_11360"
     CDS             <1..198
                     /gene="rpsO"
                     /locus_tag="PLM74_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HON43706.1"
                     /translation="PEVQVAILTERINYLTEHLKTHKKDHHSRRGLLKMVGRRRALLD
                     YLKDADIERYRAIVERLGLRK"
     gene            285..>1681
                     /locus_tag="PLM74_11365"
     CDS             285..>1681
                     /locus_tag="PLM74_11365"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HON43707.1"
                     /translation="MRQTFETIIAGRKLSVDTHYLAGQAGGSALVRYGDTVILVTATA
                     SKEPREGIDFFPLLVDYEERLYAVGKIPGGFIKREGRPSEAAILAGRQIDRPIRPLFP
                     EGFRNDVQVVATVMSVEPGVEPSVLAMNGASIALSISDIPFDGPIAGVKVGLVDGGFV
                     INPSAEQLDRSSLSLVVAGTRDAVLMVEAGANEIPEEQVLEAIMRGHDTIREIIAFQD
                     EIIAAVGVPKREFVVFEPDPKLAAKVEAFAYSRMDEAVRNPDKVTREELVGGVRQETM
                     AHFEEEMGEDTPHKDIEAVLDRMLKQIVRKMIAVEHIRPDGRALDEIRQISCEVGLLP
                     RVHGSAMFKRGQTQVVTACTLGAVGEMQIIDDLGLEESKRYIHHYNFPPYSVGEVRPI
                     RGPGRREIGHGALAERALLPVIPSEEEFPYTIRLVSEVVESNGSTSQASVCGSTLSLM
                     DAGVPIRKPVAGVAMG"
BASE COUNT          366 a          445 c          551 g          319 t
ORIGIN      
        1 ccggaagtcc aggtggcaat cttgacggaa aggataaact accttacgga gcacctgaag
       61 acgcacaaga aggaccacca ctccaggcgg ggtctgctca agatggtcgg ccgaaggcga
      121 gcgctcttgg attacctcaa agatgctgac atcgagaggt acagggccat tgtggagcga
      181 ctggggttgc gcaagtaggg gaagtgctac ggagagcggg tgtcaatctt cccgctctct
      241 tgcttgtaga cgacgcgcta cgtattgagg aggtaggtga ctgaatgcgt caaacgttcg
      301 agacgatcat agcaggaagg aagctgtcag ttgataccca ttacctcgcg gggcaggccg
      361 gggggtctgc actggtgcgg tacggcgata cggtgattct ggtcactgcg acggcttcca
      421 aggaaccgag agagggcatc gacttctttc cgcttctagt tgactacgag gagaggctat
      481 atgcagtagg caagatcccc ggagggttca tcaagcgcga gggtcgtcca agcgaagctg
      541 ccattctggc cggcaggcag atcgacaggc ccatacgccc tctgtttccc gagggcttca
      601 gaaatgacgt tcaggtggta gccacagtca tgtcggtgga accaggggtg gagccttcgg
      661 tgcttgcgat gaacggcgct tctattgccc tcagcatatc cgacataccc ttcgacggac
      721 ccattgccgg agtaaaagtg ggccttgtgg acggtgggtt cgtgatcaac cccagcgcag
      781 agcagctgga taggtccagc ttgtcgctgg tcgttgcggg tacccgggac gcggtcctca
      841 tggttgaagc cggcgccaat gagatcccgg aggaacaggt gctggaggcc atcatgcgcg
      901 ggcacgatac catccgggag ataatcgctt ttcaagacga gataatcgcc gcagtcggtg
      961 tgcccaaacg ggagtttgtg gtgttcgagc ccgatccgaa gcttgccgcg aaagtcgagg
     1021 cgttcgctta ttcacggatg gacgaggccg tgcgaaaccc cgacaaggtg acgcgtgaag
     1081 agctggtggg gggcgtcagg caggagacca tggctcactt cgaagaggag atgggcgaag
     1141 atactcctca caaagacata gaagccgtgc tggacaggat gctaaagcaa atagtccgga
     1201 agatgatcgc tgtcgagcac attcggccag acggcagggc ccttgatgag atccgccaga
     1261 ttagctgcga ggtgggcctg ctcccgcgcg tccacgggtc ggctatgttc aagcgtggcc
     1321 agacgcaggt agtcaccgca tgtacgctgg gagcagtcgg cgaaatgcag atcattgacg
     1381 atctgggcct agaggagtcc aagcgctaca tacaccacta caacttcccc ccttacagcg
     1441 tcggcgaggt acgtcccatc cggggtccgg gcaggcgcga aatcggccac ggggcccttg
     1501 cggagcgggc gcttctgccg gtcatccctt cggaggagga gtttccctat accatcaggc
     1561 tggtatccga agtagtggaa tccaacggct caaccagcca ggcgtctgtg tgcggcagca
     1621 ctctttcgct gatggacgcg ggcgtgccta tccgaaaacc agttgccggg gtcgccatgg
     1681 g
//
LOCUS       DAOTAC010000349         1634 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_55455_length_1634_cov_4.899937, whole genome shotgun sequence.
ACCESSION   DAOTAC010000349 DAOTAC010000000
VERSION     DAOTAC010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55455_length_1634_cov_4.899937"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..1208
                     /locus_tag="PLM74_11370"
     CDS             66..1208
                     /locus_tag="PLM74_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HON43708.1"
                     /translation="MERLHVEVADLSIAKHGEELCGDRVEITRNGGGVTVVLSDGLGS
                     GVKANILATLTTTIAASMLRGGAAIEDVVDTIGHTLPICKVRQVAYSTFTILQIDSDG
                     NAYLVQFDNPDAFYVADGRVNSVSCVRRQVADKVVKEARFRVSAGDHLFLVSDGIIHA
                     GIGGVLNLGWGWPNVADYIRRITQQEKRASMLARWMINVCDQFYGGKPGDDATCVAIT
                     VREPRYVTAAVGPPRNPEDDPKMVARLMGSPGTRVVCGGTTGKIVARETGAKLVVDLA
                     SMAPGVPPTATIRGTDLVTEGIITLSKAVRKLASPEEAYGRDGASRLARVLLDADHVE
                     FLVGKAVNPAHQNPNLPISITLKEQVMQDIARILSELGKEVKIAYY"
     gene            1239..>1634
                     /locus_tag="PLM74_11375"
     CDS             1239..>1634
                     /locus_tag="PLM74_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HON43709.1"
                     /translation="MTPVEVAQRKFERVNEIIAEHGREQSALIPILQEVQAEYRYLPE
                     EILTYIATAMGLSAATVFGVATFYAQFSLEPKGKYVVRVCDGTACHVRHSMLVYQAIR
                     SKLGLKDGKFTTQDMMFTVETVACLGACGV"
BASE COUNT          365 a          413 c          537 g          319 t
ORIGIN      
        1 gctgagacca aactcctatt gacgcagctc atcaggcaga tgcaggatga ggagttggat
       61 gagtgatgga gagactgcat gtggaggttg cagacctctc tatcgcaaaa cacggcgagg
      121 agctatgcgg cgaccgtgtg gagatcaccc gaaacggcgg aggggtgact gtagtgctca
      181 gcgatgggct gggctcgggg gtaaaggcga atatcctcgc aaccctcaca accaccattg
      241 ccgccagcat gctccgcggc ggcgccgcga tagaggacgt agtcgatacg ataggccaca
      301 ctctgcccat atgtaaggtg cggcaagtgg cctactcaac gtttacgatt ctccagatcg
      361 actcggacgg caatgcatac ctggtgcagt tcgacaatcc tgacgctttc tacgtcgcgg
      421 acgggcgtgt gaattcggta agctgcgtcc ggcgtcaagt ggccgacaag gtcgtgaagg
      481 aggcccggtt tagggtgagt gccggcgacc acctgtttct cgtgtcggac ggcataatcc
      541 acgcagggat aggcggcgta ctcaatctgg ggtggggttg gcccaacgtg gccgactaca
      601 tccgccgtat tacgcagcag gagaagcgcg catcgatgtt ggctagatgg atgataaacg
      661 tgtgcgatca gttttatggg ggaaagccgg gcgatgatgc gacctgcgtg gccatcaccg
      721 tgcgtgaacc ccgttatgtt acggcggcgg tgggtccgcc cagaaacccc gaggacgatc
      781 caaagatggt ggcaagactg atggggtcgc ccggaacgcg ggtggtatgc ggcggaacta
      841 ccggcaagat cgtggcccgc gagacgggcg caaaacttgt ggtggacctg gctagcatgg
      901 ccccgggcgt gccgcccact gcgactattc gaggcactga cctggttacc gagggcatta
      961 tcacgctatc gaaagccgtg aggaagctgg caagccctga ggaagcatac ggcagagacg
     1021 gcgccagcag gctggcgcgg gtcttgctag atgcggatca tgtggagttc ctggtgggaa
     1081 aggcggtgaa ccctgcgcat cagaacccga acctgcccat cagcattact ctcaaggagc
     1141 aagtgatgca ggacattgcc aggattctta gcgagctcgg gaaggaagtc aagattgcct
     1201 attactgatc catatctgat ctggggtgag gaggaagcat gacacctgtt gaagtggcgc
     1261 agaggaagtt cgagcgcgtg aacgagataa tcgcggagca cggtcgtgag cagtccgctc
     1321 tcatacctat tcttcaagag gtccaggcgg agtacaggta tcttccagag gagatcctga
     1381 cctacatcgc tacggccatg ggtctgtcgg ctgccacagt gttcggcgtg gctacattct
     1441 atgcccagtt ctcgctggag cccaagggca aatatgtggt gagagtgtgc gatggcacgg
     1501 catgccacgt gaggcattcg atgttagtat accaggcgat ccgttccaaa ctggggctta
     1561 aagacggaaa attcacaacg caggacatga tgttcaccgt agagacggtt gcgtgccttg
     1621 gtgcgtgcgg agtt
//
LOCUS       DAOTAC010000350         1594 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_57229_length_1594_cov_4.876543, whole genome shotgun sequence.
ACCESSION   DAOTAC010000350 DAOTAC010000000
VERSION     DAOTAC010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57229_length_1594_cov_4.876543"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="PLM74_11380"
     CDS             <1..685
                     /locus_tag="PLM74_11380"
                     /inference="COORDINATES: protein motif:HMM:NF028456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HON43710.1"
                     /translation="GHGDYHCIVYTPASIQEMVDLTMQAFDVAEKYRMPVMILGDAVL
                     GQMMEPVSFPDPMPFGADSVNWATTGARDGRAPRLINSIYLKPDELFRHNLKLQARFE
                     QAKAAEVRYEAVNTDDADVILVAYGTTSRVCRSAMDRARAAGIRVGLVRPITVSPFPY
                     QAIADLARTARAFLTVEMSLGQMVEDVRLAVCGAAPVHFYGTTGGVVPTASAIAEEAR
                     KIAMAEVEK"
     gene            682..1437
                     /locus_tag="PLM74_11385"
     CDS             682..1437
                     /locus_tag="PLM74_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HON43711.1"
                     /translation="MKQVYAIPKSLQPRATHYCPGCTHGIAHKLVAETIDELGVRDRA
                     IGVAPVGCAVFAYDYFDCDFQQAAHGRAPAVATGIRRVVPDAVVFTYQGDGDLASIGA
                     AEIVHAAMRGERITVIFINNAIYGMTGGQMAPTTLVGQKTTTSPAGRELGHAGSPIRI
                     AEMLATIPGAAYIARVSLTSPANVARAKKAIRKAFDTQLSGAGFSLVECLSTCPTNWG
                     MAPIEAMEWLEKNMIPYYPLGEFRTPEGGASNG"
     gene            1430..>1594
                     /locus_tag="PLM74_11390"
     CDS             1430..>1594
                     /locus_tag="PLM74_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007935256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HON43712.1"
                     /translation="MVEELIIAGFGGQGVMLMGQLLSYAGMREAKNVSWMPSYGPEMR
                     GGTANCAVVIS"
BASE COUNT          323 a          468 c          507 g          296 t
ORIGIN      
        1 agggcacggc gactaccact gtatcgtgta cacgcctgca tcgatacaag agatggtgga
       61 cctgactatg caggcattcg acgtggctga gaaataccgg atgcctgtga tgatactggg
      121 cgacgcagta ctggggcaga tgatggaacc cgtgtcgttt cccgatccta tgcctttcgg
      181 cgccgattct gtgaactggg ccacgaccgg ggcgcgcgac gggcgggctc ccaggttgat
      241 caactcgatc tacctcaagc cggacgagct cttcaggcac aacctcaagc tccaggcgcg
      301 gtttgagcaa gccaaagctg cggaggttcg gtatgaggct gtcaacactg acgacgccga
      361 tgtcatactg gttgcgtacg gcactacctc cagagtgtgc aggtcagcca tggaccgagc
      421 ccgtgcggca ggcatccgcg tcggcctcgt aaggccgatc acggtatcgc ccttcccgta
      481 tcaggcaatc gccgacctgg ctcgcacggc gcgtgcgttc ctcactgtgg aaatgagctt
      541 ggggcagatg gttgaggacg tgaggctagc tgtttgcggg gctgcgcccg tacatttcta
      601 tggtaccaca ggcggagtcg tgccaacggc ctcggcaata gctgaagagg cgcgcaagat
      661 agctatggcg gaggtggaaa agtgaagcag gtatacgcta ttccaaaatc tctgcaaccc
      721 cgggcgacac actactgccc cggctgcacc catggtattg cccacaagct agttgcggag
      781 accatcgacg aactgggcgt gcgcgaccga gctataggcg tggcaccggt aggatgcgcc
      841 gttttcgcct acgactactt tgactgcgat ttccagcagg ctgcccacgg gagggctccc
      901 gccgttgcca ccggcatcag gcgagtcgtg cccgacgcgg tcgtcttcac ctaccagggc
      961 gacggggacc tcgcctcgat cggtgcggca gaaatcgtcc acgccgccat gagaggcgag
     1021 cgcataaccg ttatcttcat caacaacgct atctacggca tgaccggggg tcagatggcc
     1081 cctactacgc tggtggggca gaagacaacc acatctccgg caggccggga gctggggcac
     1141 gcgggcagtc ccatccgcat cgccgaaatg ctggctacga tacccggagc cgcctacatt
     1201 gcccgtgtgt ccctgacatc gccggcgaac gtggcgcgcg ccaagaaggc cattcgaaag
     1261 gcctttgaca cacagctttc gggagcgggg ttttcgctgg tggagtgcct gtccacgtgc
     1321 ccgaccaact gggggatggc gccaatcgag gccatggagt ggctggagaa gaacatgatt
     1381 ccatactacc ccctgggcga gttccgtacc ccggaaggag gggctagcaa tggttgagga
     1441 gctgatcatt gcaggattcg gcggccaggg agtgatgctc atgggccaac tcctctctta
     1501 tgcaggcatg cgcgaggcta agaacgtctc gtggatgcca tcatatgggc cggagatgcg
     1561 cgggggcaca gccaactgcg ccgttgtaat atcc
//
LOCUS       DAOTAC010000351         1554 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_59245_length_1554_cov_4.983989, whole genome shotgun sequence.
ACCESSION   DAOTAC010000351 DAOTAC010000000
VERSION     DAOTAC010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59245_length_1554_cov_4.983989"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(229..891)
                     /gene="hisIE"
                     /locus_tag="PLM74_11395"
                     /pseudo
     CDS             complement(229..891)
                     /gene="hisIE"
                     /locus_tag="PLM74_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288209.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
     gene            complement(888..>1554)
                     /locus_tag="PLM74_11400"
     CDS             complement(888..>1554)
                     /locus_tag="PLM74_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HON43713.1"
                     /translation="EGADELVLLDIAATEEGHRTAMDVVRRVSSALSIPLTVGGGIAS
                     LDDIERVLEAGASKVGINTAAVQNPELVRLAADAFGRDRIVVAIDATAAAPDVHTCND
                     DAEGKWVVCTCGGKQRTRLDLVEWARTVERLGAGEILLTSIERDGAADGYDLRQLRAV
                     TETVGVPVIASGGAGVPEHFRDAFVEGGADAALAASVFHFGLLRIGEIKRYLASEGVD
                     VRI"
BASE COUNT          291 a          507 c          446 g          310 t
ORIGIN      
        1 cgggcccgct gagctgctgc aggcagccga ggatctgaag aagctggcag atgaggcgtc
       61 ccggtacgac ctgcgtaatg cgcccatacg aaggattccg gagtgcaacc gctgctattt
      121 cgcgcaattg tgcgggttca ccgcacagga tccttgtggt ttcttcgatg acagccctga
      181 ggatgaagac gacatttcgc ctgggagttg accggaccta gctgcgtgtc ggcgagccaa
      241 ctcatccacg accgcttgtg gcggaatgtc gtggtaggcc aggacgagta gggagtgaaa
      301 ccaaaggtcc gccgtctccc tgatgatctc gtctctgtcg ctattcttgg aggcgagcat
      361 aacttcgccc gcctcttctg caaccttctt cagcacccgg tcctgccccg agatgagcag
      421 cttagccgtg taggagcctt caggcttgct gactttcctg tcctggacga tgcgatagag
      481 ctcgtccagg ctgaactcgg acgtagttcc gccgatggcg caccgcggtc cggcggaaag
      541 cctcgtgaag aaacacgact tctgccctgt gtgacaggcg gcgccggtct ggtctacctg
      601 tatgagcagg caatctcggt cgcagtcgca gtagactgct cgaacgtcct ggaagtgtcc
      661 agacgtttcg cctttgaccc acagggcgtt gcgacttcgg ctccaaaagc atgtccggcc
      721 ctcgcacacg gtcttctcga tggactcgcg gctcatgtac gcgaccatga gaacctgaag
      781 cgagccagca tcctgaacaa cggcgggtat cagcccgtca tcatcgaact tcagcgtatc
      841 gacccagccc ttgaggcccg tgcaacccgg cgattcgtat tgctccgtca tatcctgacg
      901 tcaactcctt cacttgccag ataccgcttg atttcgccaa tgcgaagcag gccgaaatgg
      961 aacaccgacg cggcgagggc ggcatccgct cctccctcta caaaggcatc gcggaagtgc
     1021 tcgggcacgc ccgcgccgcc cgaggcgatc accggaacgc caactgtctc ggttaccgca
     1081 cgcagctggc gcaagtcata cccatcagcg gcgccgtctc gctcgatgga tgtaagcagg
     1141 atttcgcccg cgcccagccg ctcgacggtt cgagcccatt ctaccaggtc aagccgggtg
     1201 cgctgcttgc ccccgcacgt acacacgacc cacttgcctt ccgcgtcgtc attgcacgta
     1261 tgcacatccg gcgctgctgc agtagcgtcg atcgccacca cgatgcgatc gcgcccaaag
     1321 gcgtcggctg ccagtcgcac cagttcggga ttttgtactg cggcggtgtt gatgccaacc
     1381 ttggacgcgc ctgcctccag cacccgctca atatcgtcga gactggctat gcccccgccc
     1441 accgtgagag gaatggagag ggcggatgat accctgcgca ctacatccat ggcggtccta
     1501 tggccttctt cggtggccgc gatatcgagc agcaccagct cgtctgcgcc ttcc
//
LOCUS       DAOTAC010000352         1528 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2043
            NODE_60600_length_1528_cov_2.825526, whole genome shotgun sequence.
ACCESSION   DAOTAC010000352 DAOTAC010000000
VERSION     DAOTAC010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864110
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,282
            CDSs (total)                      :: 2,249
            Genes (coding)                    :: 2,219
            CDSs (with protein)               :: 2,219
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 6 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60600_length_1528_cov_2.825526"
                     /isolate="AMR_MDS_2043"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..974)
                     /gene="pilM"
                     /locus_tag="PLM74_11405"
     CDS             complement(<1..974)
                     /gene="pilM"
                     /locus_tag="PLM74_11405"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HON43714.1"
                     /translation="MPIGLFGRRAKSMVGLDIGTSRVKLVELTAKGVSVELTRAAVEL
                     TPVDVIRDGKVVRPEQVAAVIKKALAAGHFRPGEVVAAIAGQGVVVRHVQFPKMPEEE
                     LREALKWEGQKYIPFPMEEAVVDFEVIHGIAEPEAANMEVMLVAAQRSIVDSHLAAIE
                     LAGLRVVAVDVQPFTVLRALRYEPSGVESPCSMSNGVVAYVDIGAGTTDIVIAEGEIL
                     RFTRIVPVGGYSFTRAIADGLGVGFEEAEQIKIDEARVYQDQSQLNALDARSRAIAEA
                     VLDIAREIGTEVRRSLDYHDLQLQGRRDDAAVSRVVLAGGGSVLDGLD"
     gene            complement(1044..>1528)
                     /locus_tag="PLM74_11410"
     CDS             complement(1044..>1528)
                     /locus_tag="PLM74_11410"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HON43715.1"
                     /translation="VVAVHGVSLVSLKYLIFVSLLTIVAGTDINTRLIPNAVTYPGMA
                     VGLILSLFIPGISFLQSIVGLLVCGGVVYLLALASRGGMGGGDIKLMGMMGAFLGWDA
                     GLLALFVGALLGSVVGIARIALGKQKRRDPMPFGPFLAVGGLISAFFAPSILSFIFLQ
                     "
BASE COUNT          345 a          527 c          402 g          254 t
ORIGIN      
        1 tgatctagcc catcgaggac cgacccgcct ccggccagca ccacacggct caccgcggcg
       61 tcgtcccttc ttccctgaag ctgcaggtca tggtagtcca gcgacctacg cacctcagtg
      121 cctatctcgc gggcaatatc cagcaccgcc tcggcaatag cacgactgcg ggcgtccaac
      181 gcattcagct gcgactggtc ttgatacact cgggcttcat cgatcttgat ctgctcagcc
      241 tcttcgaacc ctacgccgag cccatcggca atggctctgg tgaagctgta gccccccaca
      301 ggcacaatcc gggtgaacct gaggatctca ccctcggcga tcacaatatc tgtggtgcct
      361 gcgccaatgt cgacgtaggc gaccaccccg ttggacatag agcacgggct ctcgactccc
      421 gagggttcat atctgagcgc ccgaagcacc gtgaacggct gaacatccac ggcaaccacc
      481 ctcaggcccg ccagttctat cgccgctagg tgggagtcga cgatgctgcg ctgcgccgcg
      541 acgagcatga cttccatgtt cgcggcctca ggttccgcga tgccgtggat gacctcgaag
      601 tcgacaaccg cctcttccat gggaaaaggt atgtacttct ggccctccca cttcagagcc
      661 tcgcgcagtt cctcttcagg catcttcgga aactgcacgt gccgcacgac cactccctgc
      721 ccggcaatgg ccgccaccac ctcgcccggc ctgaaatggc ccgcggcaag cgccttcttg
      781 atcacagccg ccacctgctc gggccggacc accttcccat cgcggatgac gtccacgggg
      841 gtaagctcga ccgccgcgcg cgttagctcg acagacacgc ctttcgcggt cagttccaca
      901 agcttgaccc ggcttgtgcc gatatcaagc cctaccatgc tcttagcgcg ccttccgaaa
      961 agaccgatcg gcatcgattc atccccccaa gacagaaaac tacaaaacga cacattagct
     1021 attcgactgt aatagataaa ctcctactgc aaaaagatga aagacaggat agacggggcg
     1081 aaaaacgcgg aaatcagccc tccgacagcg agaaacgggc caaagggcat cgggtctctc
     1141 ctcttctgct tacccagggc gatgcgcgca atccccacaa ccgagcccag gagcgccccg
     1201 acaaacagcg ccaatagacc cgcatcccac cccagaaacg cccccatcat gcccatgagc
     1261 ttgatatctc ccccgcccat gccgccccga ctggccaagg cgagcaggta gaccacgccc
     1321 ccgcacacca ggaggccaac gatggactgc aggaaggaga tgccggggat gaacaaagac
     1381 aggatgaggc cgacagccat accgggatac gtgaccgcgt tggggatgag acgggtgttg
     1441 atgtcggtgc ccgcgacgat cgtaaggagg gagacaaaga tcagatactt gagactcacg
     1501 agactgaccc cgtgaaccgc cacgacgg
//
